LOCUS       DATANS010000001         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_503899, whole genome shotgun sequence.
ACCESSION   DATANS010000001 DATANS010000000
VERSION     DATANS010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503899"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /locus_tag="VHT22_00005"
     CDS             complement(<1..110)
                     /locus_tag="VHT22_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEX3135956.1"
                     /translation="MLNVAVQMDPIERINIRGDSTFALLLEAQARGHALS"
     gene            212..>1520
                     /locus_tag="VHT22_00010"
     CDS             212..>1520
                     /locus_tag="VHT22_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3135957.1"
                     /translation="MKLPSLSIAAKLYTIFALLATVTVALSLMAVLNAQNNTTLSKEF
                     ERAFLGTQNVERVNGLIYAVVMESRGIYMSPDIPAAKRYGTLLLQFNERIANVVADWR
                     KHVGGDDAAQFAEFDKRIQQFMDFRKELVRLGTEVAPAKGREWGDNEANRSVRTALNK
                     DLDTLAQIYDTRGKKIYAELEAGVARTAWLLGVLSVVCVLLAVSGIIIIGRAVARPLR
                     NITRVTEEVASGATGVTIPHANRQDEVGALARSIVIFQQAIERNAELNRTIAEDVKAR
                     EERNAHIRSAVESFRASVEQALGAVARNAETMRSTAQTLTDVSANAKEHSGSAATASD
                     DTATNVNTVASASEELTASIQEIARHVTQATTVVREAGATTESSAAEIEGLAAAGQRI
                     GAVIDLIQAIAAQTNLLALNATIEAARAGDAGKGFAVVAQEVKS"
BASE COUNT          296 a          495 c          494 g          235 t
ORIGIN      
        1 tagctgaggg cgtggccgcg cgcttgggcc tcgagcagca gcgcgaacgt cgaatcgccg
       61 cgaatattga tccgctcgat cgggtccatc tggaccgcaa cattcagcat cggttccacc
      121 tgattttggc gaaaggacca caagcgcaaa cggaaggtta acggattacc gtcaatttga
      181 gggacctaag ctgcccgggg gtcacaagtc catgaaattg cccagtcttt cgattgccgc
      241 caagctctat acgatctttg cgctgctcgc gaccgtgacc gtcgctctct cgctgatggc
      301 ggtcctgaat gcgcagaaca acacgacgct cagcaaggag ttcgagcgcg cgttcctcgg
      361 cacgcagaac gtcgagcgcg tcaacggcct gatttacgcg gtggtgatgg aatcgcgcgg
      421 catctacatg tcgccggata ttccggccgc gaagcgctac ggcacgctgc tgctgcaatt
      481 caacgagcgc atcgcaaacg tggtcgccga ttggcgcaag catgtcggcg gggacgacgc
      541 cgcgcagttc gccgagttcg acaagcgcat ccagcaattc atggacttcc gcaaggagct
      601 ggtgcggctc ggcaccgaag tcgctcccgc gaaaggccgc gagtggggtg acaacgaagc
      661 aaaccgcagc gtgcgcaccg cgctgaacaa ggacctcgac accctcgcgc agatctacga
      721 cacccgcggc aagaagatct atgccgagct cgaagcgggt gtcgcgcgca ccgcgtggct
      781 gctcggcgtt ttgtcggtcg tctgcgttct gctcgccgtc tcgggcatca tcatcatcgg
      841 acgcgcggtg gcgcggccct tacgcaacat cacgcgtgtc accgaagagg tggcgagcgg
      901 cgcgaccggc gtcaccatcc cgcacgccaa tcgccaggac gaggtgggcg cgctcgcccg
      961 ctcgatcgtg atcttccagc aggcgatcga gcgcaacgca gagctcaacc gcaccatcgc
     1021 cgaggacgtg aaggcgcgcg aagagcgcaa cgcgcatatc cgcagcgcgg tcgaatcctt
     1081 ccgcgcttcg gtcgagcagg cgctgggcgc ggttgcacgc aacgccgaga cgatgcgcag
     1141 caccgcgcag acgctgaccg atgtgtccgc caacgccaaa gagcattccg gctcggcggc
     1201 gactgcttcc gacgacaccg cgacgaacgt caacacggtg gcatcggcct ccgaagagct
     1261 gacggcttcg atccaggaga tcgcgcgcca cgtcacgcag gccaccaccg tggtgcgcga
     1321 agccggcgcc accacggagt cgtccgcggc cgagatcgaa ggcctcgcgg ccgcggggca
     1381 gcgcatcggc gcggtgatcg atctcattca ggcgatcgcg gcgcaaacca acctgctggc
     1441 gctcaatgcc acgatcgagg cggcgcgcgc cggcgatgcc ggcaagggat ttgcggtggt
     1501 ggcgcaggag gtgaagtcgc
//
LOCUS       DATANS010000002         1660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_587874, whole genome shotgun sequence.
ACCESSION   DATANS010000002 DATANS010000000
VERSION     DATANS010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587874"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="VHT22_00015"
     CDS             <1..1091
                     /locus_tag="VHT22_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX3135958.1"
                     /translation="NWQITQMPQVESALVSVRPNDGAILSLVGGFDFDRNKFNHVTQA
                     YRQPGSSFKPFIYSAALEKGFSPATVVNDAPLYFDAAQTGGEPWEPKNYDGTFDGPMR
                     LRTALMKSKNLVTVRVMQAIGPQYAQDYIARFGFDPKLHPAYLTMALGAGAATPLQMV
                     AAYAVFANGGYRVTPYLIDRVVDGRGNVLSKATPAVAGVNAEPAIDPRNAFIMTTMLR
                     DVVRAGTGARANQLGRHDLAGKTGTTNDSVDAWFCGFNASMVAVAWIGFDQPRTLGNN
                     ETGAVAALPIWMAFMAKALKGVPETPLKQPEGVITARVNADSGLRESDDRGGISEYFF
                     SEFMPRKRDDALATDGGRAPPEVRNQLF"
     gene            1096..>1660
                     /locus_tag="VHT22_00020"
     CDS             1096..>1660
                     /locus_tag="VHT22_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3135959.1"
                     /translation="MASHIASSQGLRNRVRIAQAAARLIAEHGLSDWAAAKRKACREL
                     GLSDREALPANEEIEQALREYNNLFHPHAQAASLRAQRMIALEWMERLSSWRPLLVGG
                     VAAGWATEHSDVRLELEAEDPKAVELGLINAGIEYTAAAQANGAAPGARLNAGRGAAA
                     VRLDIVSPQQRRNRPRRNDEERLSLSDL"
BASE COUNT          293 a          544 c          566 g          257 t
ORIGIN      
        1 gcaactggca gatcacccag atgccgcagg tcgagtcggc gctggtctcg gtccggccca
       61 acgacggggc catcctatcg ctggtcggcg ggttcgattt cgaccgcaac aagttcaatc
      121 acgtgaccca ggcataccgg cagccggggt ccagcttcaa gcccttcatc tactcggccg
      181 cgctggaaaa gggcttcagc ccggcgaccg tcgtcaacga tgcaccgctc tacttcgacg
      241 cggcgcagac cgggggcgag ccctgggagc cgaagaatta cgacgggact ttcgacgggc
      301 cgatgcggct gcgcaccgcg ctgatgaaat cgaagaatct ggtaacggtg cgggtaatgc
      361 aggcgatcgg cccgcaatac gcgcaggatt acattgcgcg cttcggcttc gatccgaagt
      421 tgcatccggc gtatctgacg atggcgctgg gcgccggtgc cgcgacgccg ctgcagatgg
      481 tcgccgcgta tgcggtattc gccaacggcg gctatcgcgt gactccctat ctgatcgaca
      541 gagtcgtcga cggccgcggc aacgtgctgt cgaaggcgac gcccgccgtg gcgggggtca
      601 atgccgagcc ggcgatcgat ccgcgcaacg cctttatcat gaccacgatg ctgcgcgacg
      661 tcgtccgcgc cggcaccggg gcccgcgcca accagctcgg ccggcacgac ctcgccggca
      721 agacgggcac gaccaacgac agcgtcgacg cgtggttctg cggcttcaat gcatcgatgg
      781 tcgccgttgc ctggataggg ttcgatcagc cgcgtacgct cggcaacaac gagaccggcg
      841 cggttgcggc attgccgatc tggatggcgt tcatggcgaa ggcgctcaag ggcgtgcccg
      901 aaacaccgct caagcagccc gaaggcgtca tcaccgcgcg ggtcaatgcg gattcaggcc
      961 tgcgcgaaag cgacgaccgc gggggtattt ccgaatactt cttctcggag ttcatgccgc
     1021 gcaagcgcga cgatgcgctg gcgaccgacg gcggccgcgc gccgcccgaa gtgcgcaatc
     1081 agcttttctg acccggtggc atcgcatatc gcatcgtcgc aggggctccg caaccgcgtt
     1141 cggattgcgc aggcggccgc gcggctgatc gccgagcacg ggctatccga ttgggcggcg
     1201 gcgaagcgca aggcctgccg cgagctgggc ctgagcgatc gcgaagcgct gccagccaac
     1261 gaagagatcg agcaggcgtt gcgcgaatac aacaatctct tccatccgca cgctcaggcg
     1321 gcttcgttgc gcgcccagcg catgatcgcg ctggaatgga tggagcgcct gtcgtcgtgg
     1381 cgaccgctgc tggtcggcgg cgtcgccgcc ggctgggcga cggagcacag cgatgtaagg
     1441 ctcgagcttg aggcagagga tccgaaggcc gtggagctcg gcttgatcaa cgccggaatc
     1501 gagtacaccg ccgcggcgca ggccaacgga gcggcaccag gcgcgcgtct caacgccggg
     1561 cgcggcgcgg ccgcggtgcg ccttgacatc gtttcgccgc aacagcgccg caaccggccg
     1621 cgtcgcaacg acgaggaacg gctgtccctg tccgacttga
//
LOCUS       DATANS010000003         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_167998, whole genome shotgun sequence.
ACCESSION   DATANS010000003 DATANS010000000
VERSION     DATANS010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167998"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..613)
                     /locus_tag="VHT22_00025"
     CDS             complement(224..613)
                     /locus_tag="VHT22_00025"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3135960.1"
                     /translation="MRTLVSASHAVEDRWETALAHAQLSGPKFIALTVLIEAGEPLSL
                     GDLAARLTCVRSNITQLVDRLEADRLVRRVEDPTDRRGKRAEVTALGVQRQAAGERQV
                     QKVLKELDKILAGCDRQAMERMLTALL"
     gene            complement(763..>1590)
                     /locus_tag="VHT22_00030"
     CDS             complement(763..>1590)
                     /locus_tag="VHT22_00030"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003159648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HEX3135961.1"
                     /translation="ENHTPTGAFKVRGGLVYFYELLAAGPRPAGVISATRGNHRPSVG
                     YAARKNKVAATIVVPHGNSLEKNAAMRALGVELIEHGTDFQESREYAARLAIERGLHM
                     IPAFHPHIVRGVATYALELLRGVPDLATVYVPIGQGSGISGMIAARDALGLKTEIVGV
                     VSAHAPAYKLSFESRKAVEHAVTTELADGMACRVPDAAALDIIWRGAARVLEVTDAEV
                     AAAMRLYFSATHSTAEGAGAAPLAAALQERVRNGGKRIAVVLTGGNVDRDVFARVLA"
BASE COUNT          296 a          533 c          472 g          289 t
ORIGIN      
        1 agcgcgaacg cgctggcctt aaccaaagca cagcgctgct ctgtaaacaa ataatttggt
       61 cgcaccacat gcctcccgga caaatcacca gaccgatttc gcagccttat tggacgtcga
      121 ctgcgcaaca gaattccgcg aacggactgg ccctaccgat tacgaacgaa taaatctccc
      181 gacaaatttc ttgtcgctgt catgtatcaa tggcaggaaa agtttacaaa agcgcggtca
      241 gcatgcgctc catcgcctgg cgatcgcatc ccgcgagaat tttgtcgagc tctttcagaa
      301 ccttctgcac ctggcgctcg ccggccgcct ggcgttgcac gcccagtgcc gtgacctccg
      361 cccgctttcc tcgccggtcg gtcgggtctt cgacgcggcg gaccagccgg tcagcctcga
      421 gacgatccac gagctgggtg atgttcgacc ttacgcaggt cagcctggcg gcgagatcgc
      481 ccagactcaa gggttcaccc gcctctatca gcaccgtcaa tgcaatgaac ttgggaccgg
      541 ataactgcgc gtgcgccagc gccgtctccc agcgatcctc cacggcgtgc gatgcgctga
      601 ccagcgtgcg catcagttgc cgggtttccg agacgggagc gggcttaatg gcggccgcag
      661 accttctatt catatatgaa taatacaggc ctgatatgtc aaggtcaaga tcgccgccgc
      721 ggactgtggg attaatgctg ccggatgacc gggtgcgcgt gtttaggcaa gcacgcgcgc
      781 aaatacatcc cggtccacgt tgccgcccgt caatacgacc gcgatgcgtt tgccgccatt
      841 gcgcacccgc tcctgcaatg cagcggcaag cggcgcggcg cccgcgcctt cggccgtgct
      901 gtgcgtcgcg ctgaaataca accgcattgc tgccgcgact tccgcgtcgg tcacctcgag
      961 cacgcgtgcc gcgccgcgcc agatgatgtc gagcgccgcc gcatcgggca cgcggcaagc
     1021 catgccgtcg gcaagctcgg tcgtgacggc gtgttcgaca gccttcctgg actcgaacga
     1081 caacttgtaa gcgggcgcat gcgccgacac gacgccaact atttcggtct tgagcccgag
     1141 cgcgtcgcgc gccgcgatca tccccgagat gccggagccc tggccgatcg gcacgtatac
     1201 cgtcgcgaga tcgggcacgc cgcgcaacag ttcgagcgca taggtcgcga cgccgcgcac
     1261 gatatgcgga tgaaacgccg gaatcatgtg caggccgcgc tcgatggcaa gccgcgctgc
     1321 atattcgcgc gactcctgaa aatccgtgcc gtgctcgatg agctcgacgc cgagcgcgcg
     1381 catcgcggcg ttcttttcaa gactgttgcc gtgaggcacc acgatcgtcg ccgcgacttt
     1441 gtttttgcgc gccgcgtagc cgaccgacgg acggtggttc ccgcgcgtcg cgctgatcac
     1501 acccgcgggc cgcggcccgg cggccagcag ctcgtaaaaa taaacgagcc cgccccgcac
     1561 cttgaaggcg ccggtcggcg tgtggttctc
//
LOCUS       DATANS010000004         2823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_336008, whole genome shotgun sequence.
ACCESSION   DATANS010000004 DATANS010000000
VERSION     DATANS010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336008"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..85)
                     /gene="ccmD"
                     /locus_tag="VHT22_00035"
     CDS             complement(<1..85)
                     /gene="ccmD"
                     /locus_tag="VHT22_00035"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF016856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEX3135962.1"
                     /translation="MGGYGTYVWGAYAVTLACMAVEPVLVVL"
     gene            complement(115..870)
                     /gene="ccmC"
                     /locus_tag="VHT22_00040"
     CDS             complement(115..870)
                     /gene="ccmC"
                     /locus_tag="VHT22_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HEX3135963.1"
                     /translation="MNWFRFASPASFFPLAARLTPWFGGAAAVFAAAGLYLGLIAAPT
                     DAQQGDAYRIIFVHVATAWMSMFIYLVMAFWCAMSLMFNARLAAMMAQALAPTGALFT
                     ALALITGALWGKPTWGTYWAWDARMTSELILLFLYFGYMALRQAIDEPRRADRASALL
                     ALVGAVNVPIIYFSVIWWNTLHQGASVSVTAAPKMANVMLLAMLLMTLAFWAYAFAVI
                     FVRTRAIALERDNHAAWANDAVARAPVRQGASS"
     gene            complement(887..1528)
                     /gene="ccmB"
                     /locus_tag="VHT22_00045"
     CDS             complement(887..1528)
                     /gene="ccmB"
                     /locus_tag="VHT22_00045"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX3135964.1"
                     /translation="MRRDLRLAMRSKAELGVQLLFYVIVVALVPLGVGPDPALLRALG
                     PGVLWVAALLASLLALPRLFAMDFADGTLEQMALSPYPLPALISGKIAAHWLATGLPL
                     VAASPLLGLQFGLGGDELTVLALGLLLGTPTLSLMGAVGAALTLGLRNGALLALLVLP
                     LYVPILIFGAGAVDAVRTGLGATAELSLLAAALMASLVGATLAAAAAVRMALD"
     gene            complement(1594..2208)
                     /gene="ccmA"
                     /locus_tag="VHT22_00050"
     CDS             complement(1594..2208)
                     /gene="ccmA"
                     /locus_tag="VHT22_00050"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HEX3135965.1"
                     /translation="MLESKQLAAQRGTATLFSGIDFKLAPGDALLVTGANGSGKTTLL
                     RTAAGLCHAAQGTLSWRGVPVAPFDPDLRAAVTYIGHAAALKDELTAEENLASLASLH
                     EGSAASDAIVAALALWSLERQRALPARALSMGQRRRIGLARLALVSRPLWILDEPAAA
                     LDTAGVALLCDMIGMHLARGGMAIIATHQDIALSGPSVRGLSLS"
     gene            2347..>2823
                     /locus_tag="VHT22_00055"
     CDS             2347..>2823
                     /locus_tag="VHT22_00055"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HEX3135966.1"
                     /translation="MPRSSGVAVRLARCAAGCIPALLAGLLLVPTPAGAITAIRLGPL
                     IAQVGRPFVSSFIPINSHLPKYPVVWSLSPGNCLSGSGISLDADTGTLSGTPGKAGTF
                     NCVVVAIDTFPDPITTAQRAFTLVIEPDCDEPSITSGAPPAGTAGAPYAFTVSATGR"
BASE COUNT          512 a          990 c          935 g          386 t
ORIGIN      
        1 ggagcacgac cagcaccggc tcgaccgcca tgcaggcgag cgtcaccgcg tacgcgcccc
       61 agacgtaggt gccgtagccg cccatggcga agaaatcggc ggcgctgttc cagttcacga
      121 gcttgctcct tggcgcacgg gggcgcgcgc gaccgcatcg ttcgcccacg ccgcgtgatt
      181 gtcgcgttcc agggcaatgg ccctggtgcg gacgaagatg accgcaaacg cgtaggccca
      241 gaacgcgagc gtcatcagca gcatcgccag cagcatcacg tttgccattt tgggcgctgc
      301 ggtgacgctg acgcttgccc cttggtgcaa ggtgttccac cagatcaccg agaaatagat
      361 gataggcacg ttgaccgcgc cgaccagcgc cagcagggcg ctggcgcgat cggcgcgacg
      421 cggctcgtcg atcgcctggc gcaatgccat gtagccgaag tagaggaaca gcaggatgag
      481 ctccgaggtc attcgcgcgt cccaggccca gtaagtgccc caggtcggct tgccccaaag
      541 cgcgccggtg atcagcgcca gggcggtaaa cagcgcgccg gtcggggcca gcgcctgcgc
      601 catcattgcc gccaagcggg cattgaacat cagcgacatc gcgcaccaga aggccatcac
      661 caggtagatg aacatcgaca tccacgccgt cgccacgtgc acgaatatga tccggtacgc
      721 gtcgccctgt tgcgcgtcgg tcggcgctgc gatcagcccg agatagagtc ccgccgccgc
      781 aaacaccgcc gcggcaccgc cgaaccatgg cgtgaggcgc gcagccagcg gaaagaacga
      841 cgccggtgac gcaaatcgga accagttcaa cgaagcttcc tcccgatcaa tcgagcgcca
      901 tgcgcaccgc ggccgcagca gcaagcgtcg caccgaccag cgaagccatc agcgcggcgg
      961 cgagcagcga cagctcggcc gtggcgccca ggcccgtgcg caccgcatcg accgcgcctg
     1021 cgccgaaaat caggatcgga acgtatagcg gcagcaccag cagcgccagc agcgcgccgt
     1081 tgcgcaatcc cagcgtcaat gcggcgccga cggcgcccat aagtgacaaa gtcggcgttc
     1141 ccagcaacag gccgagcgct agcacggtaa gctcgtcgcc cccgaggccg aactgcagtc
     1201 ccagcagcgg cgaggccgcc accagcggca ggccggttgc aagccagtgc gccgctattt
     1261 taccggagat cagggcgggc agcggatacg gcgacaacgc catctgctcg agcgtgccat
     1321 cggcgaaatc catcgcgaac aggcgcggca gcgcgagcag cgaggccagc agtgcggcga
     1381 cccagagcac gcccggcccc agcgcacgaa gcagcgccgg gtcgggaccg acgccgagcg
     1441 gaacgagggc gacgacgatc acatagaaca acagctgcac gccgagctcg gccttcgagc
     1501 gcattgcgag gcgcaaatcg cgccgcatcg cccacgccgc gccgcgccac gcgccgggcg
     1561 gcgacggcgc tgccgcttcg gccgtcgcgt cggtcatgac aacgacaggc cgcgcaccga
     1621 cggtcctgac aaagcgatgt cttggtgggt cgcgataatc gccatcccgc ctcgggcgag
     1681 gtgcatccct atcatgtcgc acagcagcgc gacgcctgcg gtgtccagcg ccgcagccgg
     1741 ctcgtcgaga atccagagcg ggcgcgacac cagcgccaaa cgcgccaggc cgatgcgtcg
     1801 acgctggccc atcgacagtg cacgcgccgg caacgcgcgc tgccgctcga gcgaccacag
     1861 agcgagcgcg gcgacgatcg catcgctggc ggcgctgcct tcgtgcaggc tcgcgagcga
     1921 agcgagattc tcctcggccg tcagctcgtc cttcaacgct gcggcgtggc cgatgtaggt
     1981 gacggccgca cgcaagtcag gatcgaatgg cgcaaccgga acgccgcgcc acgacaatgt
     2041 gccctgcgcg gcatgacaca ggcctgccgc ggtgcgcagc aacgtcgtct tgccgctgcc
     2101 attggcgccg gtcaccagca aggcgtcgcc gggcgccagc ttgaaatcga tgcccgagaa
     2161 taaggtcgcg gtgccgcgct gcgcggccag ctgcttcgat tccagcatcg aacctgcgcg
     2221 gcggatggaa ggggtcccga ttatagcccg gcgctgcatc gcttccccgg gaggggcggc
     2281 tgagcggcgg atgtgctaag gttgcgcccg gtcgccgttc aaatcccgcc cggctaggac
     2341 ttgtcgatgc ctcggtcgtc cggagtcgca gtgcgccttg cccgatgcgc cgccggctgc
     2401 atccccgcgc tgctggcagg gctgttgctc gttccgacgc ctgccggtgc gatcaccgcc
     2461 attcggctcg gcccgctgat cgcgcaagtc ggccgcccgt tcgtttccag cttcatcccg
     2521 atcaacagcc atctgcccaa gtacccggtg gtgtggtcgc tcagtccggg caattgcttg
     2581 tcgggatccg ggatcagcct ggatgccgat accgggacgc tgtcgggtac gccgggcaaa
     2641 gcggggacgt tcaactgcgt cgtcgtcgcc atcgacacct tccccgatcc gatcacgacg
     2701 gcgcagaggg cgttcaccct ggtgatcgag ccggactgcg acgaaccgtc gatcaccagc
     2761 ggtgcgccgc cggcgggcac tgcgggcgcg ccctatgcat tcacggtgag cgcgaccggt
     2821 cgt
//
LOCUS       DATANS010000005         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_630002, whole genome shotgun sequence.
ACCESSION   DATANS010000005 DATANS010000000
VERSION     DATANS010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630002"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VHT22_00060"
     CDS             <1..636
                     /locus_tag="VHT22_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX3135967.1"
                     /translation="DGFSAALQFAARDTSGDPNGGDNGDRLSELRHANVISGGAFYNR
                     GPLDLGVAYERNYRIRPTGPIDEALSLTGGYDFGTIASNVGLRLAGVYERLKYDTPTG
                     SLTRDFYGLSTTLALGDGTLYAFWGKAADGKGSAVDGTTIGALVKGPDTSSQQWELSY
                     SYNLSLRTMLYAGWVKINNRPNAAYTFNINPYPVAPGAKPGGVVLGIAHFF"
     gene            complement(655..>1606)
                     /locus_tag="VHT22_00065"
     CDS             complement(655..>1606)
                     /locus_tag="VHT22_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX3135968.1"
                     /translation="RDDDGTLIDPHGGVADLRAGILRHVSPAFAEDPLRVLRVARFAA
                     RFGFSVAPETEALLREIAAGGELATLTAERVWQELAKALMEARPSLFFEVLRRCGALG
                     QLLPEVDALFGVPQPPAHHPEIDAGVHLMLALDFSAAAGDPLPVRYAVLAHDLGKATS
                     DRANWPRHIAHEARSVALARRLSERLRVPADCSELARLVARYHGDVHRAAELRASTLL
                     DLLLAADALRRPERLDGLLRACSADALSRPGRFGESYAPGVRLRAALAVVRDIDAGAI
                     AAAHPDSPELPQRIRTARLKALQAWEKANDGNPRQAEPSR"
BASE COUNT          272 a          535 c          560 g          239 t
ORIGIN      
        1 gatggctttt ccgcggcgct gcagttcgcc gcgagagata cgtcaggcga tccgaatggc
       61 ggcgacaacg gcgaccggct gtcggagttg aggcacgcga acgtcatctc cggaggcgcg
      121 ttctacaaca gagggcccct cgacctcggt gtcgcctacg agcgcaatta ccggatacgc
      181 ccgaccggcc ctatcgacga ggcgctgtcc cttaccggcg ggtacgattt cgggacgata
      241 gcgagcaacg tcgggttgcg ccttgccggc gtttacgagc ggctcaagta cgatacgccg
      301 accggcagcc tgacgcgcga cttctacgga ctgtcgacga cgctggcgct cggggacggc
      361 accttatacg cgttctgggg caaggccgca gacgggaagg ggagcgcggt cgacggcaca
      421 acgatcggag cgctggtcaa aggaccggat acgtcgagcc agcaatggga gctgtcatac
      481 agctacaacc tgtcgctgcg aacgatgctc tacgctggat gggtgaaaat aaataatcgg
      541 ccaaacgcag cctacacgtt caatatcaac ccctatcccg tcgccccggg cgccaagcca
      601 ggcggagtcg tactgggcat cgcgcatttc ttctgaggtc ggctcgccat tcgcctaccg
      661 ggacggctcg gcctgccgcg gattgccgtc gttcgctttt tcccatgcct gcaaggcttt
      721 gagtcgcgct gttctgatcc gctgcggcag ctcgggcgaa tcgggatgcg ctgcggcgat
      781 ggcgccggca tcgatgtcgc gcacgacggc gagcgccgcg cgaagccgca ctcccggcgc
      841 atagctttcg ccgaagcggc ccgggcggga cagcgcatcg gcggagcagg cgcgcagaag
      901 gccgtcgagg cgttcgggcc ggcgcaacgc gtcggccgcg agcagcaggt cgagcaaggt
      961 cgaggcccgg agctccgccg cgcgatgcac gtcgccatga tagcgggcga ccagacgggc
     1021 aagctcgctg cagtcggcag gcacgcgcaa gcgctcgctt agccgccgcg ccagagcgac
     1081 gctgcgggcc tcgtgcgcga tgtgtctcgg ccagttcgcg cgatcgctcg tcgccttgcc
     1141 cagatcatgc gcgagcactg cgtagcgcac cggcaacggg tcgcccgcgg ctgcggagaa
     1201 atccagcgcc agcatcaggt gcacgccggc gtcgatctcg gggtgatgcg cgggaggctg
     1261 cggcacgccg aagagcgcat cgacttccgg cagcagctgt cccagcgcgc cgcagcgacg
     1321 caggacttcg aaaaatagcg acgggcgggc ttccatcagg gccttggcga gctcctgcca
     1381 gacgcgttcg gcggtcagcg tcgccagttc gccgccggcc gcgatctcgc gcagcagcgc
     1441 ttcggtctcc ggggcgacgc tgaagccgaa ccgtgccgcg aagcgcgcga cgcgcagcac
     1501 gcgcagcgga tcctcggcga atgccgggct gacgtggcgc aggatgccgg cacgcaagtc
     1561 ggcgaccccg ccgtgcggat cgatcagcgt cccgtcgtcg tcgcgc
//
LOCUS       DATANS010000006         1612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_294207, whole genome shotgun sequence.
ACCESSION   DATANS010000006 DATANS010000000
VERSION     DATANS010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294207"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VHT22_00070"
     CDS             complement(<1..482)
                     /locus_tag="VHT22_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEX3135969.1"
                     /translation="MAVNPLVIDGESLSLDALRRIEGSRARVVLSDQARERMKRSIEV
                     VREAVRTDRVSYGITTGFGAFANKRISADQVRQLQLNLVRSHAVGVGEPLAAPIVRRM
                     LLLKANSLAAGISGVRTELVDALLAMLNNDVLPVIPSRGSVGASGDLAPLAHMSLCLI
                     G"
     gene            complement(524..>1612)
                     /gene="ettA"
                     /locus_tag="VHT22_00075"
     CDS             complement(524..>1612)
                     /gene="ettA"
                     /locus_tag="VHT22_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX3135970.1"
                     /translation="DAESVAWLERFLKDYKGTVVAVTHDRYFLDNVAGWILELDRGRG
                     IPWEGNYSSWLEQKSNRLQQEEKAETRKQQTLERELEWVRSNPKARQAKSKARMQRYE
                     ELASQEFQKRNETNEIYIPPGPRLGELVIEAKSLKKGFGDRLLFDNLNFKLPAGGIVG
                     VIGPNGAGKTTLFRLITGQEQPDSGEVRVGPSVQLAYVDQSRQSLNDANSVWQEISGG
                     LDMIRVGNYETSSRGYVGRFNFRGTQQQQLIGELSGGERNRVHLAKVLKSGGNLVLLD
                     EPTNDLDVETLRALEEALLSFPGCAVVISHDRWFLDRIATHILAFEGDSEVVWFEGNH
                     AQYVEDLKRRKGEDADSPHRIRFKKLDA"
BASE COUNT          269 a          543 c          488 g          312 t
ORIGIN      
        1 ccgatcaagc acagcgacat gtgtgcgagc ggcgcgagat ctccggacgc gcccaccgag
       61 ccgcggctcg gaatcaccgg aagcacgtcg ttgttcagca tcgcgagcag tgcgtcgacc
      121 agttcagtgc gcacaccgga aatacccgcc gcgagactat tggccttgag cagcaacatg
      181 cgacggacga tcggtgccgc gagcggctcg ccgactccga cggcgtgact gcgcacgaga
      241 ttgagttgca gctgacgcac ctggtccgcg gagatgcgct tgttcgcgaa cgcaccgaaa
      301 ccggtggtga tgccgtaact cacgcgatcc gtgcgcactg cctcgcgcac gacttcgata
      361 ctgcgcttca tgcgctcccg cgcctggtcg ctcagtacga cgcgcgctcg cgagccttcg
      421 atgcgccgca aggcatccag cgaaagcgac tcgccgtcaa tgaccaatgg gtttacagcc
      481 atgatgtgag tgtagcgctg taggtcgggc tgcagcccga ctcctatgca tccagcttct
      541 tgaacctgat ccgatgcggg ctatccgcat cctcgccctt gcgccgcttc agatcctcca
      601 cgtactgcgc atgattcccc tcgaaccaca cgacctcgct gtcgccctcg aacgcgagga
      661 tgtgggtcgc gatgcggtcc aggaaccagc gatcgtgcga gatcaccacc gcgcagcccg
      721 ggaagctcag cagcgcttcc tccagtgccc gcagcgtttc gacgtccagg tcgttcgtgg
      781 gttcgtcgag cagcaccaga ttgccgccgc tcttcagtac tttcgcgaga tgcacgcggt
      841 tgcgctcacc acccgacaat tcgccgatca gctgctgctg ctgcgtgccg cggaagttga
      901 agcgtccgac gtaaccgcgc gaggacgtct cgtagttgcc gacgcgaatc atgtcgaggc
      961 cgccggagat ttcctgccag acgctgttcg catcgttcag cgactgccgc gactgatcca
     1021 cataagcgag ctgcaccgac ggaccgacgc gcacttcgcc gctgtccggc tgctcctggc
     1081 ccgtgatcag tcggaacagc gtcgtcttgc cggcgccgtt cgggccgatc acgccgacga
     1141 tgccgccagc aggcagcttg aaattcagat tgtcgaacag caggcgatcg ccgaagccct
     1201 tcttcaggct cttggcctcg atcaccagct cgcccaggcg cgggccgggc ggaatgtaga
     1261 tttcgttggt ctcgttgcgc ttctggaatt cctgcgacgc cagctcttcg tagcgctgca
     1321 tgcgggcctt gctcttggct tgccgcgcct tcgggttcga gcgtacccac tccagctcgc
     1381 gctccagcgt ctgctgcttc cgcgtttcgg ccttctcttc ctgctgcagg cggttcgact
     1441 tctgctcgag ccacgacgag tagttgcctt cccaggggat gccgcggccg cgatcgagct
     1501 cgaggatcca gccggcgacg ttgtcgagga agtagcgatc gtgcgtcacc gcgacgacgg
     1561 tgcctttgta gtccttgagg aagcgctcga gccaggcgac cgattcggcg tc
//
LOCUS       DATANS010000007         1773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_84505, whole genome shotgun sequence.
ACCESSION   DATANS010000007 DATANS010000000
VERSION     DATANS010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84505"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VHT22_00080"
     CDS             <1..796
                     /locus_tag="VHT22_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX3135971.1"
                     /translation="TGRIDLRNHRKIAVIDGRVAYVGSQNLVDATFKRGLTYEELNVR
                     LSGPIALELQAVFVEDWYAETSEGLGDLRYFPEPRITGDVTVQALPSGPGYPRENNQR
                     LIVSLIHAAASRVVITMPYLVPDEALLQALQTAVLRGIDVTLVVPLQMDQILVHLAQR
                     SYYDELLDSGVRICRYGTRFLHAKHVTIDDRIAWIGSSNLDVRSFALNAEIVVLLYDA
                     SVCASLAREQARYLRDGQMLDPAGWSKRPGAMKVAENMARLLTPLL"
     gene            complement(843..1622)
                     /locus_tag="VHT22_00085"
     CDS             complement(843..1622)
                     /locus_tag="VHT22_00085"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX3135972.1"
                     /translation="MSDSYENIVVETRDRVGIIRLNRPQRMNALNEALPSELGRALAA
                     FDSDAAIGAIVITGNEKAFAAGADIGAMAEWDYQKVYADNYIMSWESMRGTRKPVIAA
                     VAGYALGGGCELAMMCDLIIAADTAKFGQPEITIGTMPGMGGTQRLPRAVGKAKAMDW
                     CLTGRMIDAQEAERSGLVARVVPADKLFDEALAVAAKIAGYSLPVVLKIKEAINRAYE
                     SSLSEGLMSERREFHATFALDDQKEGMRAFVEKRKPEFKHR"
     gene            complement(1631..>1773)
                     /locus_tag="VHT22_00090"
     CDS             complement(1631..>1773)
                     /locus_tag="VHT22_00090"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase domain-containing
                     protein"
                     /protein_id="HEX3135973.1"
                     /translation="SDTMAFMFETRAVIHPTRFALESAQRQPEYYRCWATLASHFDPD
                     KP"
BASE COUNT          298 a          582 c          557 g          336 t
ORIGIN      
        1 caccggccgc atcgatctgc gcaaccaccg caagatcgcg gtgatcgacg gacgcgtcgc
       61 gtatgtcgga tcgcaaaacc tggtcgacgc gacattcaag cgcggcctca cctacgagga
      121 gctcaacgtg cgtcttagcg ggccgatcgc actcgagctg caggcggtct ttgtcgagga
      181 ttggtacgcc gaaacgtccg agggtctggg cgatctgcgc tactttccgg agccgaggat
      241 caccggcgac gtgacggtgc aagcgctgcc gagcggcccc ggttacccgc gcgagaacaa
      301 ccagcgcctg atcgtctcgc tgatccacgc ggcggcaagc cgcgtcgtca tcacgatgcc
      361 ttacctcgtt cccgacgaag cattgctgca agcgctgcag acggccgttt tgcgcggcat
      421 cgacgtgaca ctggtcgtgc cgttgcagat ggatcagatc ctcgtgcacc tggcgcagcg
      481 ttcctactac gacgagcttt tggactcggg tgtccgtatc tgccgctatg gcacgcggtt
      541 cctgcacgcc aagcatgtca cgatcgacga ccggatcgcc tggatcggat cgtcgaatct
      601 cgacgtgcgc tcgttcgcgc tcaacgccga gatagtcgtc ctgctctacg acgccagcgt
      661 gtgcgccagc ctggcacgcg aacaggcacg gtaccttcgc gacggacaga tgctcgaccc
      721 ggccggttgg tcgaagcgtc ccggggcaat gaaggtggca gagaatatgg cgcggctgct
      781 gaccccgctg ctttgatcaa cccaggcgtg cccgcgccgt ccggaagggc gtgagaccag
      841 acctagcggt gcttgaactc gggcttgcgc ttttcgacga acgcgcgcat gccttccttc
      901 tgatcgtcga gtgcgaaagt cgcgtgaaac tcgcggcgct cggacatcag gccctcggac
      961 agcgatgact catacgcgcg attgatcgct tccttgatct tcagcacgac aggcagcgaa
     1021 tagccggcga tcttggccgc caccgccagc gcttcgtcga acagcttgtc ggcgggcacg
     1081 acccgcgcga ccagccccga gcgctcggct tcctgcgcat cgatcatgcg accggtcagg
     1141 caccagtcca tcgccttggc cttgcctacc gcacgcggca agcgctgcgt cccgcccatg
     1201 ccgggcatcg ttcctatggt tatctccggc tgaccgaatt tcgccgtatc ggcggcaatg
     1261 atcagatcgc acatcatcgc cagctcgcat cctccgccga gcgcatagcc ggcgaccgcg
     1321 gcgattaccg gcttgcgcgt gccgcgcatc gactcccagc tcatgatgta gttgtcggca
     1381 tagaccttct ggtaatccca ctcggccatc gcgccgatgt cggcgcctgc ggcaaaagcc
     1441 ttttcgttgc cggtgatgac gatcgcgcct atcgcggcgt cgctgtcgaa cgcggcgagc
     1501 gcgcggccga gctcgctggg cagcgcctcg ttgagggcgt tcatgcgctg cggccggttg
     1561 agacggatga tgccgacgcg gtcccgcgtt tcgacgacga tgttctcgta ggagtcgctc
     1621 atcgggcctt tcagggtttg tccggatcga aatggctggc tagcgtcgcc cagcagcggt
     1681 aatactcggg ttggcgctgc gccgattcca gcgcgaatcg cgtcgggtgg atgaccgcgc
     1741 gcgtctcgaa catgaacgcc atggtgtcgg ata
//
LOCUS       DATANS010000008         1944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_252487, whole genome shotgun sequence.
ACCESSION   DATANS010000008 DATANS010000000
VERSION     DATANS010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252487"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..121
                     /locus_tag="VHT22_00095"
     CDS             <1..121
                     /locus_tag="VHT22_00095"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787538.1"
                     /note="class II aldolase; catalyzes the reversible aldol
                     condensation of dihydroxyacetonephosphate and
                     glyceraldehyde 3-phosphate in the Calvin cycle, glycolysis
                     and gluconeogenesis; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructose-1,6-bisphosphate aldolase"
                     /protein_id="HEX3135974.1"
                     /translation="CVARYQQFGCAGKASLIKPVPLQKMAARYKSGELRQIVN"
     gene            complement(255..1511)
                     /locus_tag="VHT22_00100"
     CDS             complement(255..1511)
                     /locus_tag="VHT22_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX3135975.1"
                     /translation="MIRRFRGWAGKLGPGLITGAADDDPSGIATYSQAGAQFGFLTLW
                     AVVFTYPLMVGIQLVSARIGRVTGHGLASNIGRHFSRPILYGIVALLVFANTINIAAD
                     IAAMGEALNLVVGGPPHAHAVFIGVSCLLLQVFLSYQRYVRYLKWLTLALLSYVVLVL
                     TIQIPWREVAEHAVRPHFALTGAYLTVIVAVFGTTISPYLFFWQASQEVEELNADPAA
                     GALAHAPEGAAEHLQRIKIDTYLGMAFSNIVAFCIILGTAATLNAAGVTDIQTSAQAA
                     SALRPLAGDLSFMLFALGIIGTGMIAVPVLAGSAAYAVAETFEWKRGLDLKLLEAREF
                     YAIIALATLGGVALDFSPIDPIRALFWAAVINGVIAVPIMVVMMLLADDPKVMGAFTV
                     TRALKALGWLATGTMAAAVVAMIATW"
     gene            complement(1659..>1944)
                     /locus_tag="VHT22_00105"
     CDS             complement(1659..>1944)
                     /locus_tag="VHT22_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3135976.1"
                     /translation="IMMIYVTMVYGPIAAFLVEMFPTRIRYTSMSLPYHIGNGWFGGM
                     LPLLATALVAAKGDIYYGLWYPIIVALISFVVGTIFLRDNMQRDIESDWK"
BASE COUNT          382 a          633 c          624 g          305 t
ORIGIN      
        1 ctgcgtcgcg cgctaccagc agttcggctg cgccggtaag gcttcgctga tcaagccggt
       61 gccgctgcag aagatggccg cccgctacaa gagcggcgag ctgcggcaga tcgtgaatta
      121 gcggcgcgag ccacccggcg aggggcccca tgcgcagcat ggggatcgac ggggaattgc
      181 gagtcgacgc tggaggccga caactcggcc atggatgttc gtcgacggcc gatgaggccg
      241 aggcgacgcg ccgctcacca ggtcgcgatc atcgcgacga ctgccgccgc catcgttccg
      301 gtcgctagcc agcccagcgc cttcaacgcg cgcgtcaccg tgaacgcgcc catgactttg
      361 gggtcgtccg ccagcagcat catcacgacc atgatcggca cggcgatgac gccgttgatc
      421 accgccgccc agaacagcgc ccgaatcgga tcgatcggcg aaaagtcgag cgcgacgccg
      481 cccaatgtcg ccagcgcgat gattgcgtag aactcgcgcg cctcgagcag cttcaggtcg
      541 aggccgcgct tccattcgaa cgtctcggcg acggcgtacg cggccgatcc ggccagcacg
      601 ggcacggcga tcattccggt gccgatgatt cccagcgcga acagcatgaa gctcaagtct
      661 cctgccagcg gtcgcagcgc gctcgcggct tgcgcggaag tctgtatatc ggtcacgccc
      721 gccgcattca gcgtcgcggc agtgccgagg atgatgcaga acgcgacgat gttggagaac
      781 gccattccga gataggtatc gatcttgatg cgctgcaggt gctcggcggc accttcgggc
      841 gcatgcgcca gcgcgccggc ggccggatcg gcgttgagct cctcgacctc ctgggaggcc
      901 tgccagaaaa acaggtacgg gctgatcgtc gtaccgaata cggcgacgat caccgtcaga
      961 tacgcgccgg tcagcgcgaa atgagggcgc accgcatgct cggccacttc gcgccagggg
     1021 atttgtatcg tcaacaccag gactacgtac gacagcagcg ccagggtcag ccatttcaga
     1081 tagcgaacat agcgctggta cgaaaggaag acctgcagca gcaggcagct gacgccgatg
     1141 aagaccgcgt gcgcatgcgg cggtccgccg acgaccaggt tcagcgcctc gcccatcgca
     1201 gcgatgtctg cggcgatgtt gatcgtgttg gcgaagacga gcagcgcgac gatgccgtag
     1261 aggatcggtc gcgaaaaatg ccggccaatg ttcgaggcga ggccgtgtcc ggtgacgcgt
     1321 ccgatacgcg cgctgaccag ctggatcccc accatcagcg gataggtgaa caccaccgcc
     1381 cacaaggtca ggaaaccgaa ttgcgcgccg gcctgcgagt aggtggcgat gcccgacgga
     1441 tcgtcgtccg cggcgccggt gatcagcccg gggccgagct tgcccgccca tccgcgaaat
     1501 ctgcgtatca tcgtcgttcc ccgcctccgg ccgggcgagt atatcggaag cggcatccgg
     1561 cgccgcccgg tccgccgcaa gtgtcggcaa aaaaaaggcc ccggtcctcg gaccggggcc
     1621 ttcgccaaac gtgcgctcga tcgagcgctt ctttgtagct acttccagtc cgactcgatg
     1681 tcccgttgca tgttgtcgcg caggaagatg gttcccacga cgaaactgat cagcgcaacg
     1741 atgatcggat accagagccc atagtagatg tcccccttgg ccgcgaccag cgccgtcgcc
     1801 agcagtggca gcatgccgcc aaaccagccg ttgccaatgt gataaggcag cgacatcgac
     1861 gtgtatcgga tccgcgtcgg gaacatctcg accaggaagg cagcgatcgg cccgtacacc
     1921 atcgtcacgt agatcatcat gatg
//
LOCUS       DATANS010000009         2007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_504590, whole genome shotgun sequence.
ACCESSION   DATANS010000009 DATANS010000000
VERSION     DATANS010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504590"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VHT22_00110"
     CDS             complement(<1..651)
                     /locus_tag="VHT22_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX3135977.1"
                     /translation="MPNQVTIRPSNHTFLVQDDETILDAALREGFVISYGCRNGACGT
                     CKGTVLSGNVDYGTYQESALTEAEKRLGLALFCQARPSGDLVIECREVNAVKDIQIRT
                     LPCRVQKMELVAGDIMVLYLKLPASERLQFLAGQYIDILMKDGSRRSLSMANGPHDDE
                     LLQLHLRNYGGPFSQHVFTTMKVRDILRFEGPFGTFFLRDDSDKPIVLLASGTGFAP"
     gene            716..1594
                     /locus_tag="VHT22_00115"
     CDS             716..1594
                     /locus_tag="VHT22_00115"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3135978.1"
                     /translation="MRRLLIIGCGDVALRALPQLRERYRIYGLTHNRARAPLLRSRGV
                     VPIVGDLDDPASLDGLAGIAHDVLHSAPPPSQGARDTRTSHLIATLAKAKSLPQHLVY
                     ISTSGVYGDCAGALVDETRSLHPQTARAQRRVDAERQLRVWGIRSGVRISILRAPGIY
                     SEERLPVKRLAAGMPALCADEDGYSNHVHAADLARIAVAALRFGHAGRMYNASDDSVL
                     KMGDYFDLVADHCGLPRPPRLSRAEASARLPSNLWSFMNESRRLSNRRLKTELRFKLR
                     YPTVRDGIAAIAPAGE"
     gene            1614..1940
                     /gene="cutA"
                     /locus_tag="VHT22_00120"
     CDS             1614..1940
                     /gene="cutA"
                     /locus_tag="VHT22_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX3135979.1"
                     /translation="MNAVVVLTNAPDRAVAEKIARLLVDRKLAACVNILAECSSVYRW
                     QGNMETAAEVPLLIKTRGDIYPEVEAAIKSVHPYELPEIVAVPVADGSREYLDWINAA
                     TVTEIG"
BASE COUNT          368 a          641 c          628 g          370 t
ORIGIN      
        1 cggcgcgaag ccggtgccgc tcgcgagcag cacgatcggc ttgtcgctgt cgtcgcgcag
       61 aaaaaacgtg ccgaacggcc cttcgaagcg caggatgtcg cgcactttca tcgtggtgaa
      121 tacatgctgg ctgaacggcc cgccgtaatt gcgcaagtgc aattgcagga gctcatcgtc
      181 gtgcgggccg ttcgccatcg acaggctgcg ccgggagccg tctttcatca ggatgtcgat
      241 gtactggccg gcgagaaatt gcagccgttc ggatgccgga agcttcaggt acagaaccat
      301 gatgtcgccg gcgacgagct ccattttctg cacgcggcac gggagcgtcc tgatctgaat
      361 atccttgacg gcgttgacct cgcggcactc gatcacgagg tcgccggacg gccgcgcctg
      421 gcagaagagc gcgaggccga gccgtttttc cgcttcggtg agtgcgcttt cctggtacgt
      481 cccgtaatcg acattgccgg agagcaccgt gcccttgcac gtgccgcagg cgccattgcg
      541 gcaaccgtac gaaatcacga agccctcgcg cagtgcagcg tcgagtatcg tttcgtcgtc
      601 ctgcaccagg aaagtgtggt tgctcggacg tatggttacc tggttgggca tgtcaacgcg
      661 taaatatagg tggtaacggc gaggagccgc ttttttaagt tagaatttgc accctatgcg
      721 acgcttactg atcatcggtt gcggcgacgt ggcgttgcgc gcattaccgc aattgcgcga
      781 acgctaccgc atttacggcc ttacccacaa ccgcgcacgc gcgccgctgc tgcgcagccg
      841 cggcgtggtg ccgatcgtgg gagacctcga tgatccggcg agcctcgacg gtctggcggg
      901 catagcccat gatgttctgc atagcgctcc accgccgtcg cagggcgcgc gcgacactcg
      961 cacgtcccac ctgatcgcaa cgctggcaaa ggccaaaagt ctaccacagc acttggtcta
     1021 catcagcacg agcggcgtct atggcgattg cgcgggcgca ctcgtcgatg agacccgctc
     1081 gctgcatccg caaaccgcgc gcgcgcagcg ccgcgtcgac gccgagcgcc agctgcgcgt
     1141 gtggggcata cgcagcggcg tgcgcatctc gatcctgcgc gcgcccggca tttactccga
     1201 agagcgcctg ccggtgaaac gcctggcagc cggcatgccc gcgctgtgcg ccgacgaaga
     1261 cggctacagc aatcacgtgc acgccgcaga tcttgcgcgc atcgcggtgg cggcgctgcg
     1321 ctttggtcat gccggccgca tgtacaacgc gagcgacgac agcgtgctca agatgggcga
     1381 ttacttcgat ctcgtggccg atcattgcgg gctgccgcgt ccgccgcgcc tgtcgcgcgc
     1441 ggaggcgagc gcgcgcctgc cgtccaatct gtggtcattc atgaacgaat cgcggcggct
     1501 gtcgaaccgc cgtctcaaaa ccgagttgcg gttcaagctg cgctatccga cggtccgcga
     1561 cggcattgcc gcaatcgcgc ctgcgggtga atgatgaacg ccttggtcat ctcatgaacg
     1621 cagtggtcgt cttaaccaat gcgcccgacc gcgcggtcgc cgaaaaaatc gcacgcctgc
     1681 tcgtcgaccg taagctcgcg gcatgcgtca acatcctcgc ggaatgctcg tcggtatacc
     1741 gctggcaggg caacatggaa accgcggccg aggtgccgct actgatcaag acacgcggcg
     1801 acatttatcc cgaagtcgag gccgccatca aaagcgtgca tccgtatgaa cttcctgaaa
     1861 tcgtcgcggt cccggtggca gacgggtcgc gcgaatacct cgactggatc aacgcggcga
     1921 ccgtcaccga gatcggctga accgaatatg gcgagactca tttccatcct cgtgctgctg
     1981 ctggcgatcg gcaccaatgc cgtcgcg
//
LOCUS       DATANS010000010         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_546548, whole genome shotgun sequence.
ACCESSION   DATANS010000010 DATANS010000000
VERSION     DATANS010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546548"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VHT22_00125"
     CDS             <1..423
                     /locus_tag="VHT22_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX3135980.1"
                     /translation="HLSFFEADAIARWAGARLPTEAEWECAAEGVALAGNFLESTALH
                     PLALREAPPEGTPAQMFGDVWEWTRSDYAPYPRFRPAQGAIGEYNGKFMCNQYVLRGG
                     SCVTPRSHIRATYRNFFPAAARWQFSGFRLARDLSRRS"
     gene            502..2061
                     /locus_tag="VHT22_00130"
     CDS             502..2061
                     /locus_tag="VHT22_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HEX3135981.1"
                     /translation="MIASSSMPIGRPRDGRRPVRPFAALLAAATLLLAPLATGFGFDD
                     VGKIAAQRAATSYKARTDKLPDALAKLDYDHFRDIRFKPDRSLWHPEGLPFEVAFFHE
                     GQNFKDPVKINELVGDVVREVRFNPELFDYGANVIDPRDLRGLGFAGFRVHYPINSPK
                     YKDEVVVFLGASYFRALGKGQRYGLSARGLAVDTALGSGEEFPQFVEFWLRRPTPGAT
                     ELTIFALLDSRRMTGAYSFVVKPGIDTAVDVKARLFLREFVSKPGFAPLTTMYFFGEN
                     QHAARDDYRPEVHDSDGLSVASGTGEWIWRPLVNPKRLLVTSFATANPSGFGIMQRDR
                     DFEHYEDLEARYDLRPSAWIEPKGLWGTGRVELVQIPSPDETNDNIVAYWVPDRTPAP
                     QQPYNIEYRILWQKDAWARPPTSWVTQTRRGRGYVRNDDGSIGFVIDFDGPALRKLPA
                     DAKIEGIASADANGEVVELIGHRNDAEGGWRLALRVKRRDTSKPVELRAYLRGGNNAV
                     SETWSYILPPE"
BASE COUNT          375 a          725 c          698 g          374 t
ORIGIN      
        1 cacctcagct tcttcgaggc cgacgcgatc gcacgctggg cgggagcgcg cctgccgacg
       61 gaagccgaat gggaatgcgc ggccgaaggc gtcgcgctgg cgggcaactt cctcgagagc
      121 acggcgctcc atcccctggc gcttcgcgag gcgccgcccg agggcacgcc cgcgcagatg
      181 ttcggcgacg tgtgggaatg gacgcgcagc gactacgccc cgtatccgcg tttcaggccc
      241 gcgcagggcg cgatcggcga gtacaacggc aagttcatgt gcaaccagta cgtgttgcgc
      301 ggcggctcgt gcgtgacacc gcgttcgcac atccgcgcga cctaccggaa ttttttcccg
      361 gccgctgcgc gctggcagtt ttccggcttc cgcctcgcgc gggatttgag tcggcgcagc
      421 tgaccttgac gaccggttgc tcgagcgatg cctgagcggc catcggcagc gccgacaact
      481 cctttgcaac ctggcagacg catgatcgcc agcagttcga tgccgatcgg aaggccacgg
      541 gatggcaggc gcccggttcg accgtttgcg gcactgctcg ccgccgcgac gttgctgctg
      601 gccccgcttg cgaccggctt cggcttcgac gacgtcggga aaatcgccgc gcagcgcgcc
      661 gcgacctcgt acaaggctcg caccgacaag ctgccggatg cgctggccaa actcgactac
      721 gatcatttcc gcgacatccg cttcaagccg gatcgctcgc tctggcatcc cgaagggctc
      781 ccgttcgagg tcgcattctt tcacgagggg cagaacttca aggacccggt caagatcaac
      841 gagctggtcg gcgacgtcgt gcgcgaagtg cgattcaatc ccgagctgtt cgactacggc
      901 gccaatgtca tcgatccccg cgacttgcgc gggctcgggt tcgcgggatt tcgcgtgcat
      961 tacccgatta attcgccgaa gtacaaggac gaggtcgtcg tgtttctcgg cgccagttat
     1021 tttcgcgcgc tcggcaaagg ccagcgctac gggctatcgg cgcgcggcct tgccgtcgac
     1081 acggcgctcg gctcgggcga ggagtttccg cagttcgtcg agttctggct cagacgaccg
     1141 acacccggtg cgaccgagct cacgattttt gcgctgctcg actcccggcg catgaccggc
     1201 gcctacagct tcgtcgtcaa gcccggtatc gataccgcag tcgatgtcaa ggctcggctc
     1261 ttcctgcgcg aattcgtcag caagcccggc tttgcaccgc tgacgacgat gtatttcttc
     1321 ggcgaaaacc agcacgcagc ccgcgacgac taccgccccg aagtccatga ctcggacggc
     1381 ctgtcggtcg catcgggaac cggagaatgg atctggcggc cgctggtcaa tcccaagcgg
     1441 ctgctggtca cgtcgtttgc gacggcgaac ccgtccggat tcggcatcat gcagcgcgac
     1501 cgcgactttg agcattacga ggatctggag gcgcgttacg accttcgccc cagcgcatgg
     1561 atcgagccga aaggcctttg gggaaccggc agagtcgagc tggtgcagat tcccagcccc
     1621 gacgagacca atgacaacat cgtcgcttac tgggtgccgg acaggacgcc tgcaccgcaa
     1681 cagccgtaca acatcgaata ccgcatcctg tggcagaagg acgcctgggc acgcccgccg
     1741 acttcgtggg tgacccagac ccggcggggt cgcggctacg tccgtaatga cgacggcagc
     1801 atcggctttg tcatcgactt cgacggtccc gcgctgcgca agctgccggc cgatgcgaag
     1861 atcgagggca tcgcctcggc ggacgctaac ggcgaggtgg tcgagctgat cggccaccgc
     1921 aacgatgcag aaggcggctg gcgcctggca ttgcgcgtca aacggcgcga tacgagcaag
     1981 ccggtcgaac tgcgcgcata tttgcgcggc ggcaacaacg cggtgtcgga aacgtggagc
     2041 tacatccttc ctcccgagta gccggcgctg acgctgacga cgacgccgac gcctgcatcc
     2101 gctacctgga tcgattgccg ctcgcgcccg agcagcgcgc tgcactgctg cggcgcatcg
     2161 cggccgacgt cg
//
LOCUS       DATANS010000011         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_588404, whole genome shotgun sequence.
ACCESSION   DATANS010000011 DATANS010000000
VERSION     DATANS010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588404"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VHT22_00135"
     CDS             complement(<1..266)
                     /locus_tag="VHT22_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3135982.1"
                     /translation="MSGARALGGTIAATLTLPAVLTLAALLTLASLPLTVLPRLTLLP
                     LLTLLSLLILLSSLALLSLLALPIALSVSLLLRVLQRPAQLRNL"
     gene            303..665
                     /locus_tag="VHT22_00140"
     CDS             303..665
                     /locus_tag="VHT22_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3135983.1"
                     /translation="MPSISLGGFEDGRGSLARQTATLSERIRDLANRMDRGDLSQTEL
                     EALRRAATQLRRLSGDPLAAQPDVMMRLINQIELQALAANAKTREASAARATIPDPDS
                     PRYREAVAEYYRRLGNRQ"
     gene            662..1492
                     /locus_tag="VHT22_00145"
     CDS             662..1492
                     /locus_tag="VHT22_00145"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX3135984.1"
                     /translation="MRLRFALAAAIVSISLGAGASSNLITGDGGRSGMPADMTKLNFA
                     RIIYDSEGGMGEAYYAYDGRVWARWETDFPEGDDNFGHRLSQLTRIQVAKHASSRMLT
                     APDLGDFPLLYMSDPGYMVVSNEEKAALARYLANGGFVWCDDFWGDAEWQQFADVMRQ
                     VLPGREWRTLTADHPIFHTVFDLNDGMPQIPALPFASPGASTAEPAGAHKFPAGNLET
                     PEMRAWLDDDGRIMVLATHNTDVGDGWEREAYGEWYFETFSTKSYMVGVNVLIYAMTH
                     "
BASE COUNT          298 a          495 c          525 g          241 t
ORIGIN      
        1 cacaagttgc ggagctgcgc cgggcgctgc aggacgcgca acagcaagct gacgctgagg
       61 gcaatcggca aggccaacag ggacagcagg gccagcgagg acaacaggat caacaaggac
      121 aacagggtca gcaagggcaa caaggtcagc cggggcaaga cggtcagcgg caggctcgcg
      181 agggtcagca gggcagccag ggtcaggacg gcaggcaagg tcagggtcgc agcgatcgtc
      241 cctccgaggg ctcgcgcccc ggacaacagc cgcgcggcgg attgacggcg tggaatccca
      301 acgtgcccag catttcgctc ggcggcttcg aagacggccg cggctcgctc gcgcgccaga
      361 ctgcgacgct cagcgagcgc atccgcgatc tggcgaaccg catggatcgc ggcgatctga
      421 gtcagaccga gctggaagcg ctgcgtcgcg cggctacaca gctgcgtcgt ctcagcggcg
      481 acccgttggc tgcgcaaccg gacgtgatga tgcgattgat caatcagatc gagctgcagg
      541 cgcttgcggc gaatgcgaag acacgcgagg catcagcggc gcgcgccacg attccggatc
      601 cggattcgcc gcgctatcgc gaggcagtcg ccgaatatta tcggcggctc ggtaatcgcc
      661 aatgagactg cgctttgcgc tcgccgcggc gatcgtttcg atctcgctgg gcgcgggcgc
      721 gtcttccaac ctgatcaccg gcgacggcgg gcgctcgggc atgcccgcgg acatgaccaa
      781 gctgaacttc gctcgcatca tctacgacag cgaaggcggc atgggcgagg cgtactacgc
      841 ctacgacggt cgcgtctggg cgcgctggga aacggacttt ccggaaggcg acgacaactt
      901 cggccatcga ctctcgcagc tgacgcgcat ccaggtcgcg aaacacgcct ccagccgcat
      961 gctcaccgcg ccggacctcg gcgactttcc gctgctgtac atgtccgacc cgggttacat
     1021 ggtcgtgtcc aacgaggaga aagccgcctt ggcgcgttac ctggcgaacg gcgggttcgt
     1081 gtggtgcgac gacttctggg gcgacgccga atggcagcag ttcgcagacg tgatgcgcca
     1141 ggtgctgccc ggccgcgaat ggcgcacgct cactgcggat catccgatct ttcacacagt
     1201 gttcgatctg aacgacggca tgccgcagat tccggcgtta ccgtttgcga gccccggcgc
     1261 gagcaccgcc gaacccgccg gcgctcacaa attccccgcc ggcaacctcg agacgccgga
     1321 gatgcgcgcg tggctcgatg acgacggtcg catcatggtg ctcgccaccc acaacaccga
     1381 cgtcggcgac ggctgggagc gcgaggccta cggcgagtgg tacttcgaga cgttctcgac
     1441 caagtcctac atggtcggcg tcaacgtcct gatctacgcg atgacgcact gacgcggtcc
     1501 gtgaaggagc gggtgtcaat ttggtaatcg ggtaatctgg tgatctggta atggccgtc
//
LOCUS       DATANS010000012         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_126709, whole genome shotgun sequence.
ACCESSION   DATANS010000012 DATANS010000000
VERSION     DATANS010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126709"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VHT22_00150"
     CDS             complement(<1..394)
                     /locus_tag="VHT22_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3135985.1"
                     /translation="MRRAALLACVAALLAAPFAAPPFWITLLNYIGLYSIVTLGLVLL
                     TGVAGQTSFGQAAFVGLGAYTTAYLSLNYPALSPWLNLVAGMALTAFVALGLGFITLR
                     MRGHYLPLATIAWGISIFYVFGNLDFLGG"
     gene            complement(399..1436)
                     /locus_tag="VHT22_00155"
     CDS             complement(399..1436)
                     /locus_tag="VHT22_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX3135986.1"
                     /translation="MDLSIAVLLAQDGVTNGAIYALLSLALVLVFAVTRVIFVPAGEL
                     VTYGTLTLASLQLGKSPGTVGLLVGLAGLAGAMEVYAATRDRDWRRLPRRLAVYLVVP
                     LAIAALVHLLAPLSLAFPLQILLAVAIVTALGPLLYRVAYQPLAEASVLVLLIVSVAV
                     HFAMLGLGLWYFGAEGSRTPAFTSASYRIGDVQISGQSLLVLLFSLLVIAALYVFFER
                     TLYGKALRATALNRVGARLVGISPNLAGSLTLTLAALLCAFSGVLIGPITTVYYDSGF
                     LVSLKGFVGAIIGGMSSYPLAAGGAFLVGLLESYSSFWASAFKEVIVFTLIIPVLLWR
                     SLTTHHHEEEE"
BASE COUNT          367 a          513 c          513 g          221 t
ORIGIN      
        1 tcccgccgag gaaatccaga ttgccgaaca cgtagaaaat gcttatcccc catgcgatcg
       61 tcgccagcgg caggtaatga cctctcatcc gcagcgtgat aaagccgagc ccgagcgcaa
      121 caaacgcggt cagggccatg ccggcgacca gattgagcca tggcgacagc gccggataat
      181 tcaacgagag gtacgcggtc gtataggcgc cgagcccgac gaaggcggcc tggccgaacg
      241 aagtctggcc ggcgacaccg gtcagcagca ccagtcctag cgtgacaatg ctatagaggc
      301 cgatgtagtt gagcagcgtg atccagaatg gcggcgctgc gaacggcgcg gccagcagcg
      361 ctgcgacaca ggcaaggagc gccgcccgcc tcatcgcgtc actcttcttc ctcgtgatgg
      421 tgtgtcgtca gcgagcgcca gagcagcacc gggatgatca gggtgaaaac gatgacttcc
      481 ttgaacgcgc tcgcccagaa cgacgagtac gattccagca ggccgaccag aaacgcaccg
      541 cccgccgcca gcggatagct cgacatgccg ccgatgatcg cgccgacgaa gcctttcaga
      601 ctcaccagga agccggagtc gtagtagacg gtggtgatcg gcccgatcag cacgccggaa
      661 aaggcgcaca acaacgcggc cagggtcaac gtcaggcttc cggcgaggtt ggggctgatg
      721 ccgacaaggc gcgcgccgac gcgattgagc gccgtcgcgc gcagcgcctt gccatatagc
      781 gtccgctcga agaagacgta gagcgcggca ataaccagca acgagaacaa cagcaccaga
      841 aggctctgtc cgctgatctg cacgtcgccg atgcggtagg acgcggacgt gaacgccggc
      901 gtgcgcgagc cttcggcgcc gaaataccac aggcccagac ccagcatggc gaaatgcacg
      961 gcgaccgaga cgatcagcag caccaatacc gatgcctcgg ccagcggttg gtaggcgacg
     1021 cgatagagca gcggcccgag cgcggtgacg atcgcgaccg cgagcaatat ctgcagcgga
     1081 aaggcaagcg acagcggcgc cagcagatgg acgagcgcgg caatcgccag cggcacgacc
     1141 agatagaccg ccagccggcg cggcagccgg cgccaatctc gatcgcgcgt tgccgcgtag
     1201 acctccatcg caccggcgag gcccgccaga ccgaccagca gcccgacggt gcccggcgat
     1261 ttcccgagtt gcagcgaagc cagcgtcagc gttccgtagg tcacgagctc gccggccgga
     1321 acgaagatga cccgcgttac cgcgaatacg agaaccagcg ccagtgaaag cagcgcatag
     1381 attgcgccgt tggtgacgcc atcctgcgcc agcagtactg cgatcgagag gtccatcgga
     1441 tatcggtagc ctcggcagcg agcagaaaac aaaaggccgg ctacggtgtc ccgcagccgg
     1501 cctggtgagc gtcagctcat ttgctcagga cccacttgcc gttttcgatc ttgacgataa
     1561 cgcgggcgcg gttgtccata ccattgtgat cggatgcatt catgctgaaa atgc
//
LOCUS       DATANS010000013         2995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_336685, whole genome shotgun sequence.
ACCESSION   DATANS010000013 DATANS010000000
VERSION     DATANS010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336685"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VHT22_00160"
     CDS             complement(<1..232)
                     /locus_tag="VHT22_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3135987.1"
                     /translation="MPVPGLAAPAERTVTNPSASAMLDLQAAPTVGIVRDPKERGCEE
                     PIIEEIRIMKRILIALLAAVALCGVAAYSWADG"
     gene            325..1077
                     /gene="ompR"
                     /locus_tag="VHT22_00165"
     CDS             325..1077
                     /gene="ompR"
                     /locus_tag="VHT22_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HEX3135988.1"
                     /translation="MKQLTPKILVVDDDPRLRELLVRYLSEQGFQVQALPDARELDKK
                     LQRDPPHLLVLDLMLPGEDGLAVCRRLRGSGETVPIIMLTAKGEDIDRIVGLEMGADD
                     YLPKPFNPRELVARIHAVLRRQIERLAPGAPVGEGRIEFGPYVLDLTTRTLTRNGTPL
                     PMTTGEFGLLKVFVTHARQPLAREKLMLLARGRDHDVFDRAIDVQVSRLRKLIEPDPT
                     APRYLQTVWGFGYVFVPDEVASNSPADTGAGP"
     gene            1074..2378
                     /locus_tag="VHT22_00170"
     CDS             1074..2378
                     /locus_tag="VHT22_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3135989.1"
                     /translation="MKLWPRSLFGRLALLLLMVVAVSQGTAILLYRQDRAALVARQFS
                     DTKVLQLQALRAALAATELHERSETLTRLGKEYGARIVSDDGGGFFGVTPQNPLLVDL
                     GERLRTDLGPETELRLQPRQQLLWIKMQAGDHAYWAGFHWPRPSDAAQSRALEWSLIA
                     LVVLLASAYAFARYLSRPLRQLNDAVANVGAGKAQPPLPEKGPSEIVNLNHGFNQMLA
                     SLRQAEQDRALLLAGVSHDLRTPLARLRLGLEVGTHDEQMREGMAADIEEMDKIIGQF
                     LDFGRDDRDVALEMRPLDEIVAPLLERYRRAGNEVHYSGGDLPALPMRATAMSRLLSN
                     LIDNALRYGGTPVDVSARSEGASVVIEVGDRGPGIPPEQVDRLKRPFTRGDPARSGAS
                     GAGLGLAIVERIARLHGARFDVLAREGGGTLARVTMPAGAGS"
     gene            complement(2375..2860)
                     /gene="ispF"
                     /locus_tag="VHT22_00175"
     CDS             complement(2375..2860)
                     /gene="ispF"
                     /locus_tag="VHT22_00175"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX3135990.1"
                     /translation="MATVRVGTGFDVHALASGRRLVLGGVTIPFDRGLAGHSDADVLL
                     HAVCDAMLGACGLGDIGMHFPDTDAAYKDADSRVLLRRVWQLASDRSFELGNIDATIV
                     AQAPKIAPYLAAMAANIAADLGCAAGSINIKAKTSEGLGFTGRGEGIAAMVSLVLFAK
                     S"
     gene            complement(2863..>2995)
                     /locus_tag="VHT22_00180"
     CDS             complement(2863..>2995)
                     /locus_tag="VHT22_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX3135991.1"
                     /translation="AQAVEALGVKPRLVQGSPANIKVTYPEDIALAEAILAGRTRTA"
BASE COUNT          503 a          995 c         1001 g          496 t
ORIGIN      
        1 cgccgtcggc ccacgaatac gcagcgacgc cgcaaagcgc gaccgctgca agcaaggcaa
       61 tcaggatgcg tttcatgatg cggatctcct cgataatggg ttcttcgcag ccccgctctt
      121 tcgggtcgcg aacgatgccg actgtaggcg ccgcctgtaa gtcaagcatt gcagaagccg
      181 agggatttgt aaccgttctt tcagcggggg cggcaaggcc tggaaccggc atcgagtgta
      241 accaatcgtt gcccctgtgg ccgggcgtta caattcgtga caagtgagaa gcgccgcgcc
      301 gcgcgcgccc gcgataatcg agcgatgaaa cagcttacgc caaaaattct ggtcgtcgac
      361 gacgatcccc ggctacgcga gctgctggta cgctatctgt ccgaacaggg cttccaggtg
      421 caggcgctgc ccgacgcgcg cgagctcgac aagaagctgc agcgcgatcc tccgcatttg
      481 ctggtgctcg acctgatgct tcccggcgag gacggccttg ccgtctgccg aagactgcgc
      541 ggctccggtg aaaccgtgcc tatcatcatg ctgacggcca agggcgagga catcgaccgc
      601 atcgtcggcc tcgaaatggg cgccgacgat tacctgccca aaccgttcaa tccacgcgaa
      661 ctggtcgcgc gcatccatgc ggtgctgcgc cgccagatcg agcgactggc gcccggtgcg
      721 ccggtgggcg aaggccggat cgaattcggg ccctatgtac tcgatttgac cacgcgcacg
      781 ctgacccgca acggcacgcc gttgccgatg accaccggcg agttcggctt gctgaaggtt
      841 ttcgtcaccc atgcccgcca gccgctggcg cgcgaaaagc tgatgctgct cgcccgcggg
      901 cgcgatcatg acgtcttcga ccgggcaatc gacgtccagg tctcgcggct gcgcaagctg
      961 atcgagcccg accccacggc gccgcgctac ctgcagacgg tctggggctt cggctatgtc
     1021 ttcgtgccgg acgaggtcgc cagcaacagc ccggccgaca ccggggccgg tccgtgaaat
     1081 tgtggccgcg ttcgctgttc ggacgcctgg ccctgctgct gctgatggtc gtcgctgtga
     1141 gccaggggac ggcgatactg ctgtaccggc aggaccgcgc cgcgctggtc gcgcgacagt
     1201 tcagcgacac aaaagtcttg caactgcagg cgctgcgcgc cgcgctggcg gcgaccgagc
     1261 tgcatgagcg cagcgagacg ctgacccggc tcggcaaaga atacggcgcg cgaatcgtca
     1321 gcgatgacgg tggtggattc ttcggtgtga cgccccaaaa tccgctgctg gtcgacttgg
     1381 gagagcgact gcggaccgac ctcggcccgg agaccgagct ccgcctgcaa ccgcgccagc
     1441 agctgctgtg gatcaagatg caggctggcg atcacgctta ctgggcgggg ttccactggc
     1501 cgcgcccgtc cgacgcggcc cagtcgcgcg cgctcgaatg gagcctgatc gcgctggtcg
     1561 tgctgctcgc cagcgcctat gcattcgccc gttacctgtc ccggccgttg cgtcagttga
     1621 acgacgccgt ggccaacgtc ggtgcgggga aggcacagcc gccgctgccg gaaaaggggc
     1681 cgtccgaaat cgtcaacctc aatcacggct tcaaccagat gctggccagt ctgcggcagg
     1741 ccgagcagga ccgcgcgctg cttctggccg gcgtctcgca cgatttgcgc acgcctttgg
     1801 cccggctgcg cttggggctg gaagtcggaa cgcacgacga gcagatgcgc gagggcatgg
     1861 cagccgacat cgaggaaatg gacaagatca tcggccagtt tctcgacttc ggccgcgacg
     1921 atcgcgatgt cgcgctcgag atgcggccgc tggacgaaat cgtcgcgccg ctgctcgagc
     1981 gttaccgccg cgccggcaac gaggttcact acagcggcgg tgaccttccg gccctgccga
     2041 tgcgagccac cgcgatgtcg cggctcttgt ccaatttgat cgacaacgcg ctgcgctacg
     2101 gcggcacgcc ggtcgacgtt tccgcccgca gcgagggcgc atcggtggtg atcgaagtag
     2161 gcgatcgcgg ccccggcatc ccgccggagc aggtcgatcg cctcaagcgt ccgttcaccc
     2221 gcggcgatcc ggcgcgaagc ggcgccagcg gggctgggct ggggctcgcg atcgtcgagc
     2281 gcattgctcg gctgcacggc gctcgcttcg atgtgctcgc gcgcgaaggc ggcggcacgc
     2341 tggcgcgcgt cacgatgccg gccggcgcgg gatcctaact cttcgcaaac aggacgagcg
     2401 agaccattgc ggcaatgcct tcgccgcgtc cggtgaaacc gagaccttcg ctcgtcttgg
     2461 ccttgatgtt gatcgagccg gctgcgcaac cgagatcggc cgcgatgttc gcggccatcg
     2521 ccgcgagata cggcgcgatc ttgggcgcct gagccacgat cgtcgcgtcg atgttgccga
     2581 gctcgaacga gcggtcagaa gcaagctgcc agacgcggcg cagcagcacg cgactgtcgg
     2641 catccttgta cgccgcgtcg gtgtcgggga agtgcattcc gatatcgccc agtccgcacg
     2701 caccgagcat cgcgtcgcag accgcgtgca gcagtacgtc ggcatcggaa tggccggcca
     2761 gcccgcggtc gaacggtatg gtgacgccac cgagcaccag gcgacggccc gacgcgagcg
     2821 cgtggacgtc gaagcccgtt ccgacgcgca ccgtggcgat gatcaggccg tccttgtgcg
     2881 tccggcgagt atcgcctcgg ccagcgcaat gtcttcggga taggtaactt tgatgttggc
     2941 gggactgcct tgtaccaggc gcggcttgac gcccagcgcc tcgaccgcct gcgcc
//
LOCUS       DATANS010000014         1785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_294609, whole genome shotgun sequence.
ACCESSION   DATANS010000014 DATANS010000000
VERSION     DATANS010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294609"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VHT22_00185"
     CDS             complement(<1..578)
                     /locus_tag="VHT22_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX3135992.1"
                     /translation="MNNNALNAISPIDGRYADKAAELRPLFSERGLIRQRVRVEALWL
                     KALVAEPGIVELRGLPAAALTVLDALAAELTEAEAAEVKRIERETNHDVKAVEYFVKA
                     RLDSVPAWRTRREFVHFACTSEDINNLSYALMLRDARDQVLLMRLDTLIGRLRALAHT
                     NAAVPMMSRTHGQPATPTTLGKELAVLAHRLRR"
     gene            657..>1785
                     /locus_tag="VHT22_00190"
     CDS             657..>1785
                     /locus_tag="VHT22_00190"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX3135993.1"
                     /translation="MANEGFRVERDSLGELQVPADALWGAQTQRALENFQISGLRMPR
                     EFIRALGLIKWAAATTNLDLDGLDAKRAAAIQKAALEVSEGRHDAHFPIDVFQTGSGT
                     STNMNANEVIAHLASRYAGVAVHPNDHVNMSQSSNDVIPTTIHLSALLSIEQHLLPAL
                     AYLGAVISRRIGDIGEVVKTGRTHLMDAMPLTFGQEMSGWHAQIDHASARIMECRVRL
                     QGLALGGTAVGTGVNAHPRFAARATRYLAVKCGLELRPAGNYFEALSSQDTAVELSGQ
                     LRALAVALMKISNDLRWMNSGPLAGIGEVKLPALQPGSSIMPGKVNPVIPEAVAMVAA
                     QVMGHDAAIAIAGQSGNFQLNVMLPLIASNLLASIHLLTNAT"
BASE COUNT          302 a          557 c          615 g          311 t
ORIGIN      
        1 cggcgcagcc ggtgcgccag cacggcgagc tccttgccga gcgtggtcgg cgtcgcgggc
       61 tggccgtgag tgcgggacat catcgggacc gctgcattgg tatgtgcgag ggcgcgaagc
      121 cggccgatca gcgtatcgag gcgcatcagc agcacctggt cgcgcgcatc gcgcagcatc
      181 aatgcatagc tcaggttgtt gatgtcctcc gaggtgcagg cgaagtgcac gaactcgcgg
      241 cgcgtgcgcc acgcgggcac ggagtccagg cgcgccttga cgaagtattc gaccgccttc
      301 acgtcgtgat tggtctcgcg ctcgatgcgc ttgacctcgg cggcttcggc ttcggtgagc
      361 tcggcggcca gtgcgtcgag cacggtgagc gcggcggctg gcaggccgcg cagctcgacg
      421 atcccgggct ctgccaccag cgccttgagc cacagcgctt cgacgcgaac gcgctgacgg
      481 atcagcccgc gctcgctgaa gagcggccgc agctcggcgg ctttgtcggc gtagcgtccg
      541 tcgatcggcg agatggcatt gagcgcgttg ttattcatag gctgtaggtc gggcttcagc
      601 ccgacaaagt actatagtac ttgagacaag cttatttttt ggcatcgagg ttgggtgtgg
      661 cgaacgaagg attcagggtc gagcgagaca gcctgggcga gctgcaagtg cccgccgacg
      721 cgctgtgggg tgcccagacg cagcgcgcgc tcgagaattt ccagatcagc ggcctgcgca
      781 tgccgcgcga gttcatccgt gcgctcggcc tgatcaagtg ggccgcggcg acgacgaatc
      841 tcgacctcga cggcctggac gccaagcgcg ccgccgccat ccagaaggcg gcgctcgaag
      901 tgtcggaagg ccggcacgac gcgcacttcc cgatcgatgt gttccagacc ggctccggca
      961 ccagcaccaa catgaatgcc aacgaggtga tcgcgcacct cgcgagccgc tacgccggtg
     1021 ttgccgtgca tcccaacgat cacgtcaaca tgagccagag ctcgaacgac gtgattccga
     1081 ccacgatcca cttgagcgcg ctgctctcga tcgaacagca cctgttgccg gcgctggcgt
     1141 acctcggtgc ggtgatcagt cggcgcatcg gcgatatcgg cgaggtcgtg aagaccggcc
     1201 gcacgcacct gatggatgcg atgccgctga cgtttggtca ggagatgagc ggctggcacg
     1261 cgcagatcga ccacgcgagt gctcgcatca tggaatgccg cgtgcgtctg caaggactcg
     1321 cgctcggcgg caccgcagtg ggcacgggag tgaatgcgca tccgcgcttt gccgcgcgcg
     1381 ccacgcgtta tctcgcggtg aagtgcggtc tcgagctgcg gcctgccggc aattatttcg
     1441 aagcgctgtc gtcgcaggac actgcagtcg agttgtctgg tcagttgcgc gcgctggcgg
     1501 tggcgctgat gaagatctcc aacgacctgc gttggatgaa tagcggaccg ctcgcgggca
     1561 tcggcgaggt caagctgccg gcgctgcagc cgggcagcag catcatgccg ggcaaggtga
     1621 atccggtgat ccccgaggcc gtggccatgg tggcggcgca ggtgatgggc cacgatgcgg
     1681 ccattgcgat tgccgggcag tcgggcaatt tccagctcaa cgtgatgttg ccgctcatcg
     1741 ccagcaatct gctcgcgtcc attcacctcc tgaccaacgc gacgg
//
LOCUS       DATANS010000015         1728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_168648, whole genome shotgun sequence.
ACCESSION   DATANS010000015 DATANS010000000
VERSION     DATANS010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168648"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VHT22_00195"
     CDS             complement(<1..229)
                     /locus_tag="VHT22_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3135994.1"
                     /translation="MLDRRTFMSLIATAAAMPQAAWAQSKPGKVALYANVGAVLTRYE
                     VDVDALTLTARESVTLPAGVQYAWPHVSRRYL"
     gene            complement(262..>1728)
                     /locus_tag="VHT22_00200"
     CDS             complement(262..>1728)
                     /locus_tag="VHT22_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX3135995.1"
                     /translation="RRTVAADAGAQMNDLHWLTAAELAGAYAARKVSPVELVQSLLGR
                     IDALEPQLNAFIKLDRDAVLAEAKEAEREIRAGGQRGPLHGVPVGIKDIIDVAGQATT
                     CHSKIYLQNIARADAEVVSRLRAAGAIPFGKLSLHEFAIGGPSFDLPFPPARNPWNRD
                     HHPGGSSSGSGTALAAGFLPLALGTDTGGSIRNPAGNCGVVGLKPTYGLVSRRGVFPL
                     AFTLDHIGPMARTVRDTALALDVLAGHDAADPGSAAISHGNFGADLERGVRGLRVGFV
                     RHFHEQDVPAHPEVTAALEEAARQFTREGALVRDLQLPPLQELAGVSRIFMLAESWTV
                     HAPWLRDRPGDYAETSRRKLMGGAFLGAGDYVHAQQRRLQLSDAVNAALRDVDVLLCA
                     NSLDPACRLDDDAELARTYPRQARSPFNLTGHPALALMCGLSTGGLPISAQIVGRMHD
                     EVTVLRAGAAYERATDWHTRRPPLENTSKAKRKPAAVS"
BASE COUNT          288 a          577 c          573 g          290 t
ORIGIN      
        1 acagataacg ccgcgaaaca tgcggccacg catattgcac gcccgccggc agcgtgacgc
       61 tttcgcgggc agtcagggtc agtgcgtcca cgtcgacttc gtagcgcgtg agcaccgcgc
      121 cgacgtttgc gtataacgcc actttgccgg gcttggactg cgcccacgcc gcctgcggca
      181 tcgcggcagc ggttgcgatc aatgacatga aggttcgccg atcgagcatg atttctcctc
      241 cctcgtggat gggtttacgg gtcagctgac tgcggccggc ttgcgcttcg ctttgctggt
      301 gttttcaagc gggggcctac gcgtgtgcca gtcggtcgcg cgctcgtagg cggcacccgc
      361 gcgcaatact gtgacttcat cgtgcatgcg gccgacgatt tgcgccgaaa tcggcaagcc
      421 gcctgttgac agcccgcaca tcagcgcgag cgccggatgg ccggtcaggt tgaacgggct
      481 gcgcgcctgc cgcggatagg tgcgcgcgag ttccgcgtcg tcgtcgaggc ggcacgcggg
      541 gtcgagcgag ttcgcgcaca gcaacacatc gacgtcgcgc aatgcggcgt tgacggcatc
      601 cgacaattgc aggcggcgct gctgggcgtg cacataatcg cccgccccaa gaaacgcccc
      661 gcccatcaat ttgcggcgcg acgtttctgc gtagtccccg ggccggtcgc gcaaccacgg
      721 cgcgtgcacc gtccacgact cggcgagcat gaatatacgc gacactccgg caagctcttg
      781 cagcggcggc aactggaggt cgcggaccaa tgcgccttcg cgcgtgaact gtctcgcggc
      841 ctcctcgagc gcggccgtta cttcgggatg ggccggcacg tcctgttcgt ggaaatgccg
      901 cacgaatccc acgcgcagac cgcgcacgcc gcgttccaga tccgcgccga agttgccatg
      961 agaaattgcc gcgctgccag gatcggcggc gtcgtggccc gccaatacgt cgagcgccag
     1021 cgccgtatcg cgcacggtgc gcgccatcgg cccgatatgg tcgagcgtga atgcgagcgg
     1081 aaacacgccg cgacgcgaca cgaggccgta cgtcggcttc aagccgacga cgccacaatt
     1141 gcccgcggga ttgcggatcg agccgccggt atcggtgccc agcgcaagcg gcagaaaacc
     1201 ggcggcgagc gccgtgccgg aacccgacga cgagccgccg ggatgatgat cgcggttcca
     1261 cggattgcgc gccggcggaa acggcagatc gaacgacggg ccgccgatcg cgaattcatg
     1321 cagcgacagc ttgccgaagg ggattgcgcc cgccgcgcgc aggcggctca cgacttcggc
     1381 gtccgcgcgc gcgatgttct gcaggtaaat tttcgaatgg caggtcgtcg cctgccctgc
     1441 cacgtcgatg atgtctttga tgccgaccgg tacgccatgc agcggccccc gttggccgcc
     1501 cgcgcgtatc tcgcgctcgg cttccttcgc ttcggcgagc acggcatcgc ggtcgagctt
     1561 gatgaacgcg ttcagttgcg gttccagcgc atcgatgcgc ccgagcagcg actgcaccaa
     1621 ttcaacgggt gaaactttgc gcgccgcgta ggcgccggcg agttcggcgg ccgtcaacca
     1681 atgcaagtca ttcatttgcg cacctgcatc ggcggccacg gttcggcg
//
LOCUS       DATANS010000016         4950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_546923, whole genome shotgun sequence.
ACCESSION   DATANS010000016 DATANS010000000
VERSION     DATANS010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546923"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1382
                     /locus_tag="VHT22_00205"
     CDS             <1..1382
                     /locus_tag="VHT22_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3135996.1"
                     /translation="HTGEAVLRAVRPDDIDPEFYYWAQASRAGKRVAVIDPVQAVAAS
                     GIDGVQLIEWGLHDRTFEVSSEPPQLLEDIRATYGDHPIRSCDLHGQTAEGYRRLLQG
                     LLRGVRAKARFTPEILAREAWDLFSVTYSESHCAGHQFWHFIDPRHPWHDPKAPHEFQ
                     HALLDVYAAIDGALPALLASAGPEARVFAIFSHGIDLYYDGPQLLPEVLVRLGLAGGS
                     LGMAGRLLRRLRTRVTYLPRPVKAAIKRLARTRAMAAPAAAAGCAVDPFASAKTRAAF
                     VNNNRCGGIRLNLRGREPFGSVEPGSEAAALLQMLRRELLALRDPKSGEAIVTRVQTS
                     SEAFGPDHHPDLPDLICVFRTDLGMLERCESPAVGLVHAPIYHPHAPRSGDHTVNSQL
                     WVSGPDIVGTRENGRGNVLDIAPTILAALRVALPDWLDGRPLPVSAAAQESLRPNDGL
                     APSALSAAE"
     gene            complement(1395..1562)
                     /locus_tag="VHT22_00210"
     CDS             complement(1395..1562)
                     /locus_tag="VHT22_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3135997.1"
                     /translation="MHYLNPTATLLLESCDGTLPAIELPGAETLAPGDVGIRAKRALA
                     EGRANDAAGPH"
     gene            complement(1665..2516)
                     /locus_tag="VHT22_00215"
     CDS             complement(1665..2516)
                     /locus_tag="VHT22_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX3135998.1"
                     /translation="MRSRTPDADPGIVAPITATVTPASEIEALTGDPNFMSSLARGLA
                     VIRAFSQQRRELTIAQLSHRTDIPRAAVRRCLYTLAKLGYVGTEDGRTYALRPRILAL
                     GHAYLSSTPLATAVQPLLDQISDALHESSSMAVLEGDEILYIARSSTTTRLMSIDLGL
                     GSRLPAYCTSMGRVLLADLAPVQLDQYLSRVKLTRLTNRTVSTAAELKRVLAEVRRDG
                     YAIVDQELEIGLRSIAVPVKDAAARCVAAINVGTQSARVSVAEMQARFLAPLQAAASE
                     LSVLLVR"
     gene            2721..3419
                     /locus_tag="VHT22_00220"
     CDS             2721..3419
                     /locus_tag="VHT22_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HEX3135999.1"
                     /translation="MIDKTVASPSAAVADVQDGATVMIGGFGTAGMPSELIDALIAQG
                     ARELTIVNNNAGNGEVGLAALLKAKRVRKIICSFPRQTDSYVFDALFHAGEIELELVP
                     QGNLAERIRAAGAGIGAFYTPTAYGTPLAAGKETRLIDGRGYVLEYPIAADFALIKAE
                     KGDRWGNLIYRKTARNFGPIMASAAKCAIAQVREIVPLGALDPEVIVTPGIFVQRVVA
                     VDAGARRAQPQAAA"
     gene            3430..4113
                     /locus_tag="VHT22_00225"
     CDS             3430..4113
                     /locus_tag="VHT22_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HEX3136000.1"
                     /translation="MKRFSREQLAARVARDIPEGAYVNLGIGLPTTVANHLPSDREIF
                     LHSENGLLGMGPAPAPGSEDPDLINAGKQPVTLLTGGAYFHHADSFAMMRGGHLDICV
                     LGAFQVSVEGDLANWHTGAKDAIPAVGGAMDLAIGAKRVFVMMDHQTKSGASKIVERC
                     SYPLTGVRCVNRIYTDLAVIDVTPQGLRVVEVVDGLAFDELQRLTGVPLMSADAGTAG
                     RRTATLSER"
     gene            4113..>4950
                     /locus_tag="VHT22_00230"
     CDS             4113..>4950
                     /locus_tag="VHT22_00230"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX3136001.1"
                     /translation="MQQAWICDAVRTPFGRYGGALAALRADDLGAVPIKALVERNAGV
                     DWSAIDDVVYGCANQAGEDNRNVARMSLLLAGLPVEVPGTTVNRLCGSSMDAIASAAR
                     AIKAGEATLMIAGGVESMSRAPFVMPKAESAFSRANAVYDTTIGWRFVNKLMKEMYGV
                     DSMPETAENVAVDHKIEREAQDRMALASQLKAVAAQKSGFFGGEITPVTIAQKKGDPV
                     VVAKDEHPRETSLEALAKLKGVVRPDGTVTAGNASGVNDGACALLLADEGTAARYGLT
                     PRA"
BASE COUNT          833 a         1596 c         1702 g          819 t
ORIGIN      
        1 tgcacaccgg cgaagcagtg ctgcgcgcag tgaggccgga cgacatcgat ccggagttct
       61 attactgggc gcaggcgagc cgcgccggca agcgcgtcgc cgtcatcgat ccggtgcaag
      121 ccgtcgccgc gagcggcatc gacggcgttc agctgatcga atggggcttg cacgatcgca
      181 cgttcgaggt atcgagtgag ccgccacaac tgctcgagga cattcgcgcc acctacggcg
      241 accatccgat ccgaagctgt gacctgcacg gacaaacggc cgaaggttat cgtcggttgt
      301 tgcagggcct gctccgcggc gtgcgcgcca aggcccggtt cacgcctgag attcttgcgc
      361 gcgaggcctg ggatttgttc agcgtcacct attcggagag ccattgcgcc ggtcatcagt
      421 tctggcattt catcgatccg cggcaccctt ggcacgatcc gaaggctcct cacgaattcc
      481 agcatgcgtt gctcgatgtc tacgcggcga ttgacggcgc gctgccggcg ctgctcgctt
      541 cggcgggacc cgaagcccgc gtcttcgcga tattcagcca cggcatcgac ctgtactacg
      601 acggtccgca actactgccc gaagtgctgg tgcgcctggg tctggccggg ggaagcctcg
      661 gaatggcggg cagactgttg cgtcgactcc gcacccgggt gacctatttg ccgcgaccgg
      721 tcaaggcggc gatcaagcgg ctggcgcgca cgcgcgcgat ggctgcgccg gccgccgcgg
      781 ccggctgcgc ggtcgatcca ttcgcttcgg cgaagacgcg cgcggcattc gtcaacaata
      841 accgttgcgg aggcatcagg ctcaatctgc ggggcaggga gcctttcggc agcgtcgagc
      901 cgggttcgga ggccgccgcg ctgctgcaga tgctgcggcg cgagttgctg gcgctgcgcg
      961 atccaaagtc gggcgaagcg atcgtcacgc gcgtccagac ctcgagcgaa gcgttcggcc
     1021 cagaccatca tccggaccta cccgatctaa tctgcgtgtt ccgtaccgac ctcggcatgc
     1081 tcgagcgctg cgaatcgccg gcggtcggcc tggtccatgc tccgatctac catccccatg
     1141 cgccgcgcag cggcgatcac acggtcaatt cgcagctctg ggtcagcgga ccggacattg
     1201 tcggcacgcg agagaacggg cgcggcaatg tgctggacat cgctcccacg atactcgctg
     1261 cgctacgcgt tgcgctgccc gattggctcg acggccggcc gttgccggtg tcggccgctg
     1321 cgcaggagtc cctgcgtccg aacgatggcc tcgcgccgtc cgcgctgtct gccgccgaat
     1381 agcctgaaca gcgcctagtg cggacccgcc gcgtcgttgg cgcggccctc ggccagtgcg
     1441 cgcttggcgc gtatgccgac gtcgccggga gccagtgtct cggcaccggg aagctcgatg
     1501 gcggggagtg taccgtcaca actctcgagc agcagggtcg ccgtcggatt cagatagtgc
     1561 aatcggtcgc ggccggcgtc gtagacaacc gggccgtccg gcgcctcgcg gatctcgatg
     1621 ccgggggccg ctcgaggcag atattccagt ccatcgcagc ggggctagcg cacgagcagg
     1681 acggaaagct cgctcgccgc cgcctgcagc ggcgccagga accgcgcctg catttcggcg
     1741 accgatacgc gcgccgactg cgttccgacg ttgatcgcgg cgacgcagcg cgctgccgcg
     1801 tccttcaccg ggacggcgat cgagcgcagg ccgatctcca gctcctggtc gacgatcgcg
     1861 tagccgtcgc gcctgacctc ggcgagcacg cgcttcaatt cggccgcggt ggacacagtg
     1921 cgattggtca atcgcgtcag ctttacccgc gacaggtact gatcgagttg caccggcgcc
     1981 aggtcggcaa gcagcacgcg gcccatcgac gtgcagtagg cgggcaggcg actgcccagc
     2041 cccaggtcga tcgacatcag gcgcgtcgtc gtcgatgagc gcgcgatgta gagaatctcg
     2101 tcgccttcca gcacggccat cgagctcgat tcgtgcaacg cgtcgctgat ctgatcgagc
     2161 agtggctgca ccgcggtcgc cagcggcgtc gacgacaggt aggcatgacc cagtgcgaga
     2221 atccgtggcc gcagcgcgta tgtcctgccg tcttcggtgc cgacgtagcc cagcttggcc
     2281 agcgtgtaca ggcagcggcg caccgcggcg cgcggaatgt cggtgcgatg gctgagctgc
     2341 gcgatcgtga gctcgcgtcg ctgctgcgaa aaggcgcgga tcacggcgag tccgcgcgcg
     2401 agcgagctca tgaaattcgg gtcgccggta agtgcctcga tttcggacgc cggcgtgacg
     2461 gtagctgtaa tcggcgcgac aatgcctggg tcggcgtcgg gggtccgact gcgcatggca
     2521 gcggggatgg cggatttggc ggctcgcggc atctgtaggt ctccggacac gtacgcggtc
     2581 gatgttagca ggtgggcggt aaccgcacaa gaatgcgatt atcgcccttg accgattcgg
     2641 ccagggtcgc gtagactttg ttcgttcacc gcacgattgt gcgattatcg cacgaatccc
     2701 cagtgcccag gagttggcaa atgatcgaca aaaccgtggc atcgccgtcc gctgccgtcg
     2761 ccgacgtgca ggatggcgcc acggtgatga tcggcggctt cggcacggcc gggatgccat
     2821 cggaactgat cgacgcgctg atcgcgcaag gcgcccgcga actgacgatc gtcaacaaca
     2881 atgcgggcaa cggtgaggtg ggactggccg ctttgctcaa ggccaagcgc gtgcgcaaga
     2941 tcatctgctc gtttccgcgc cagaccgatt cgtatgtgtt cgatgcgcta ttccacgccg
     3001 gcgagatcga gctcgagctg gtcccgcagg gcaacctggc cgagcgcata cgcgcggcag
     3061 gtgccggcat aggcgcgttt tatacgccga cggcatacgg tacgccgctc gctgcgggca
     3121 aggagacgcg actgatcgac ggccgcggct atgtgcttga atatccgatc gcggccgact
     3181 ttgcactgat caaggcggag aagggcgatc gctgggggaa cctgatttat cgcaagaccg
     3241 cgcgcaactt cggaccgatc atggcatctg cggccaaatg cgcgattgcg caggtgcgtg
     3301 aaatcgtgcc gctcggcgcg ctcgatcccg aggtcatcgt cacgcccggc atattcgtgc
     3361 agcgagtcgt cgccgtcgac gcgggcgcgc ggcgcgcgca gccgcaggcg gcggcttgag
     3421 gaccagacca tgaaacgttt cagccgcgag cagctggccg cacgagtcgc acgcgacatt
     3481 cccgagggtg cgtacgttaa cctgggaatc ggcctgccga cgacggtcgc caaccatctg
     3541 ccttccgatc gcgaaatttt cctgcacagc gagaacgggc tgttgggcat gggcccggcg
     3601 ccggcgccgg ggtcggaaga tcccgacctg atcaatgccg gcaagcaacc ggtgaccttg
     3661 ctgaccggag gcgcgtattt ccatcacgcc gattcgtttg cgatgatgcg cggagggcac
     3721 ctggacatct gcgtgcttgg cgcgtttcaa gtctcggtcg agggcgacct cgccaactgg
     3781 cacaccggcg ccaaggacgc gattcccgcc gtcggcggcg cgatggatct ggcgatcggc
     3841 gccaagcgcg tattcgtgat gatggatcac cagaccaagt cgggcgcatc caagatcgtc
     3901 gagcgctgca gctacccgct gaccggcgtt cgctgcgtca atcgcatcta taccgacctt
     3961 gcggtcatcg acgtcacgcc gcaggggctg cgcgtggtcg aggtcgtcga cgggctggcc
     4021 ttcgacgaac tgcagcgact gaccggcgtg cctctgatga gcgccgatgc agggacggcc
     4081 ggacgcagga ccgcgactct ttcggagcga tgatgcaaca ggcatggatt tgcgatgcgg
     4141 tgcgcacccc gttcgggcgc tacggcgggg cgcttgcagc gcttcgcgcc gatgatttgg
     4201 gcgccgttcc gatcaaggcg ctggtcgagc gcaatgccgg cgtcgactgg tcggccatcg
     4261 acgacgtcgt ctacggctgc gccaaccagg cgggcgagga caaccgcaac gtggcgagga
     4321 tgtcgctgct gctcgccgga ctgccggtgg aggtgccggg cacaaccgtc aatcgcctgt
     4381 gcgggtccag catggacgcc atcgcttcgg cggcgcgcgc catcaaggcc ggcgaggcca
     4441 cgctgatgat cgccggcggc gtcgagagca tgagccgcgc gcccttcgtc atgcccaagg
     4501 ccgagagcgc tttctcgcgc gccaacgcgg tctacgacac caccatcggc tggcgcttcg
     4561 tcaacaagct gatgaaggag atgtacggcg tcgactccat gccggagacg gcggagaacg
     4621 tcgcggtcga ccacaagatc gagcgcgagg cgcaggacag gatggcgctc gcctcgcagc
     4681 tgaaggccgt ggcggcgcag aagtcgggct tcttcggcgg cgaaatcacg ccggtgacga
     4741 tcgcgcagaa aaagggcgat ccggtcgtgg tcgccaagga cgagcatccg cgcgaaacca
     4801 gcctcgaggc cctcgccaag ctcaagggcg tggtcaggcc cgacggcacg gtgacggcag
     4861 gcaacgccag tggcgtgaac gacggcgcct gtgcgctgct gctggccgat gaaggaacgg
     4921 cggcgcgata cggcctgacg ccgcgcgcgc
//
LOCUS       DATANS010000017         3266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_42779, whole genome shotgun sequence.
ACCESSION   DATANS010000017 DATANS010000000
VERSION     DATANS010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42779"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VHT22_00235"
     CDS             <1..711
                     /locus_tag="VHT22_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136002.1"
                     /translation="QPVAKALNLKVQTTDWLTRAQLQPLGQNNPKFVQAVFSPDSLQA
                     KRNTDAIEVAPNTMMAARVIDYKPAAPRALDEVKADIRRQLEHTAASELAQKVGREKL
                     ALLQQGKDAGLVFGRPVTLARNQAQPGFQTEALARIFQGDPAKLPEYVGMPAPDGGFA
                     IYKIVQVIAAPAPDAARLSAFSARVGEQLGRELFAANLASLKAKAEVKINQAALEKDS
                     RSGEAPAPAAPSPPARRR"
     gene            complement(760..1245)
                     /locus_tag="VHT22_00240"
     CDS             complement(760..1245)
                     /locus_tag="VHT22_00240"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX3136003.1"
                     /translation="MKLRKSLWFTALCTVAIVLAAASAQAVLYKWTDENGRVVYGDQP
                     PASAKAERVNPGIAPADPNAVRSMAAKDAEIKKRQQDRADAEAKSDKEQADAKKKGDM
                     CAQARGRIRTLREDVNVYRYNEKGEKVFFEPAERQKAIADNEKMMRDLNCAPAPAAPT
                     S"
     gene            1395..2861
                     /gene="guaB"
                     /locus_tag="VHT22_00245"
     CDS             1395..2861
                     /gene="guaB"
                     /locus_tag="VHT22_00245"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX3136004.1"
                     /translation="MRVVQKALTFDDVLLLPAHSSVVPRDVSLQTRLTKTISLNIPLL
                     SAAMDTVTEAGLAIAMAQEGGLGVVHKNMSPRAQAAHVAKVKRFESGVVKDPITVPPT
                     MSVREVLALTRQHKISGLPVVDGKKVVGIVTNRDLRFETNLDQPVAKIMTQGDRLVTV
                     PEGTELELAKTLMHQHRLERVLVVNDKFELRGLITVKDILKSTEHPNACKDDLGQLRV
                     GAAVGTAGDTDERVEALIAAGVDVIVVDTSHGHSQGVLDRVRDVKRRYPKVQVIGGNV
                     ATAGGARALADQGADAIKVGIGPGSICTTRIVAGVGVPQISAILNAIDGVAALGIPLI
                     ADGGIRYSGDIAKALAAGASCVMLGSLFAGTEESPGETELFQGRSYKSYRGMGSLGAM
                     QQGSSDRYFQDSEGEGAAEKLVPEGIEGRVPYKGSIAAVVHQLMGGVRAAMGYCGCAT
                     IDALRTRAEFVEITSAGIRESHVHDVQITKEAPNYRAD"
     gene            2930..>3266
                     /locus_tag="VHT22_00250"
     CDS             2930..>3266
                     /locus_tag="VHT22_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3136005.1"
                     /translation="MPFILVVVFIDVLGIGIALPVIPMLVGLYTPSRELQAYWYGLLV
                     VAYGVMQFFCAPLLGALSDRFGRRPILLWSLLGLGVHFLLLGLAPSLPLMLLARIIGG
                     TAGASFSVAS"
BASE COUNT          549 a         1071 c         1054 g          592 t
ORIGIN      
        1 cagccggtcg ccaaggcgct caacctgaag gtgcagacca ccgactggtt gacgcgcgcg
       61 cagttgcagc cgctgggcca gaacaatccc aaattcgtgc aggcggtatt cagtccggac
      121 tcgctgcagg cgaagcggaa caccgatgcc atcgaggtcg cgccgaacac gatgatggcg
      181 gcgcgcgtga tcgactacaa gcctgccgcg ccgcgcgcgc tcgacgaggt caaggccgac
      241 atacgccggc aactcgagca caccgcggcg agcgagcttg cgcagaaggt cggcagggaa
      301 aagctcgcgt tgctccaaca gggcaaggac gccggcctcg tcttcggcag accggtcacg
      361 ctggctcgaa accaggcgca gccgggattc cagaccgagg cgctggctcg aatctttcag
      421 ggcgatccgg ccaagcttcc cgaatacgtc ggcatgcccg cacccgacgg cggcttcgcc
      481 atctacaaga tagttcaggt gatcgcagcg cccgcgccgg atgcggcccg cctgtcggcg
      541 ttctccgcgc gcgtcggcga gcagctcggt cgtgagctct tcgcggccaa cctcgcgagc
      601 ctcaaggcga aggccgaggt caagatcaat caggccgctc tggagaagga ctcgcgttcc
      661 ggcgaggccc cggcccctgc cgctccctcg cctccggcac gccgtcgctg acgcgggtac
      721 cgggcgagag ggagagcggc gcgagggcct ggcgctgcgt cagctcgtcg gggccgccgg
      781 cgccggcgcg cagttcagat cgcgcatcat cttttcgttg tcggcgatcg ctttctgccg
      841 ctcggcaggc tcgaaaaata ccttttcgcc cttttcgttg tagcgataca cgttgacatc
      901 ctcgcgcagc gtccggattc ggccgcgcgc ctgcgcgcac atgtcgcctt tcttcttcgc
      961 gtcggcctgc tccttgtcgg acttcgcctc ggcgtcggcg cgatcctgct ggcgcttttt
     1021 gatttcggca tccttggcgg ccatgctgcg caccgcgttg ggatcggctg gcgcgatgcc
     1081 gggattgact ctttccgcct tggcgcttgc cggcggctga tcgccgtaaa cgacgcgccc
     1141 gttctcatcg gtccacttgt acaggaccgc ctgggcgctc gccgccgcca gcactatcgc
     1201 aacggtgcac aaagcggtga accacagcga ctttcgcaac ttcatccagt ttccccggaa
     1261 aacgagaacc ggcacaaggt aatgccattc tctccccagc gtcaacccgc agcagtccaa
     1321 cggtcgcttc agccgtttac cgggcatgac gaggcgcgta taatccgctt ttgcgcatag
     1381 gagaaaaggt gattatgcgt gtcgttcaaa aagccctcac ctttgacgac gttctgctgc
     1441 tccctgccca ttcttccgtc gttccccgcg acgtcagtct ccagacccgc cttaccaaga
     1501 ccatttcgct gaacatcccg ctgctgtcgg cagcgatgga tacggtgacc gaagccggcc
     1561 ttgccatcgc catggcgcag gagggaggct tgggtgtcgt gcacaagaac atgtcgccga
     1621 gggcacaggc ggcgcacgtc gccaaggtca agcgcttcga aagcggcgtg gtcaaggacc
     1681 cgatcaccgt cccgccgacg atgtcggtgc gcgaagtgct ggcgttgacg cgacaacaca
     1741 agatctccgg cctgcccgtc gtcgacggca agaaagtcgt cggtatcgtc accaaccgcg
     1801 atctgcggtt cgaaaccaat ctggaccagc cggtcgccaa gatcatgacc cagggcgacc
     1861 gcctggtcac cgtgccggaa ggcaccgagc tcgagctcgc gaagacgctg atgcaccagc
     1921 accggctgga gcgcgtactc gtcgtcaacg acaagttcga gctccgcggg ctgatcaccg
     1981 tcaaggacat cctcaaatcg accgagcatc cgaacgcctg caaggacgat ctcggccagc
     2041 tccgcgtcgg cgccgctgtc ggcacggctg gagacaccga tgagcgcgtc gaagccctga
     2101 tcgctgcggg cgtcgacgta atcgtggtcg atacttcgca cggccattcg cagggcgtgc
     2161 tcgatcgggt gcgcgacgtc aaacggcgtt atccgaaggt gcaggtcatc ggcggcaatg
     2221 tggcgacggc cggcggcgcc cgggcgctgg ctgaccaggg cgcggatgcg atcaaggtcg
     2281 gcatcggtcc cggttcgata tgcacgacgc ggattgtcgc cggtgtcggc gtgccgcaga
     2341 tttccgccat tctcaatgcc atcgacggcg tcgcggcgct cggcatccca ctgatcgccg
     2401 acggcggcat tcgctattct ggcgatatcg ccaaggcgct cgccgccggc gcctcgtgcg
     2461 tgatgctggg gagcctgttc gccggcaccg aagagtcacc gggagagacc gagctctttc
     2521 aggggcgctc gtacaagagc taccgcggca tgggatcgct gggagcgatg cagcagggct
     2581 cgagcgaccg ctatttccag gattcggaag gcgaaggcgc cgccgaaaaa ctggtccccg
     2641 aaggcatcga agggcgggtg ccgtacaagg gctctatcgc cgcggtcgtt caccaattga
     2701 tgggcggggt tcgcgccgcg atgggctatt gcggctgtgc gaccatcgac gctttgcgca
     2761 cgcgcgccga gttcgtcgag attacctcgg ccggcatccg cgaatcgcac gtgcacgatg
     2821 tacagatcac caaggaagca ccgaactatc gcgccgatta gcgggcggcg ggcggggaga
     2881 aggacggcga ttggtcaagc gagttaagcc tgccggcagg caggccggga tgccctttat
     2941 cctcgtcgtt gtcttcatcg acgtgctggg catcggcatc gcccttccgg tcatcccgat
     3001 gctggtcggc ctgtacacgc ccagccgcga gctgcaggcg tattggtatg gcctgctggt
     3061 cgtcgcctat ggcgtcatgc agttcttctg tgcgccgctg ctgggcgcgc tttccgatcg
     3121 cttcggccgg cgaccgatcc tgctctggtc gctgctgggg ctcggcgttc attttctgtt
     3181 gctgggcctg gcgccatcgc tgccgctgat gctgttggca cgcatcatcg gcggcaccgc
     3241 cggtgcgagt ttttcggtgg ccagtg
//
LOCUS       DATANS010000018         2633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_42789, whole genome shotgun sequence.
ACCESSION   DATANS010000018 DATANS010000000
VERSION     DATANS010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42789"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1363
                     /locus_tag="VHT22_00255"
     CDS             95..1363
                     /locus_tag="VHT22_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3136006.1"
                     /translation="MPRRPDNRRLQPDWRALGALLKLAAQNVLRQRGRSATALIAIAL
                     GVASLIVAGGFIHDVYIQMGESIIHSQYGHLQIYRRGYVQHGTQHPTDYLIDDPDAVI
                     QRIQALPRVAIVLPRLHFTGLANAGGADWAIVGEGIEPKLENQLGTYVQLVSGRLLRE
                     DDRDAVIVGDGVAKALRLSSGQPITLSISTRAGALNTVDFQVVGIFRSYSKEFDQRSI
                     RMPLKAAKDLLATSAVNEIAVALTDTAATDATASLADASIGQLGYEVRSWRDLADFYN
                     KTVHLLEREFAFLVGVMLLMIVLSVANTINTAAFERLPEFGTMLALGDRRRDVFRLIM
                     LECTILGMLGSLLGLGIGVALALALSALGISMPPPPNTDLGYTALIRIVPGVIFGAVA
                     VGVVAAMLAGVFPSLRIARTAPAEALRMAL"
     gene            complement(1390..2103)
                     /locus_tag="VHT22_00260"
     CDS             complement(1390..2103)
                     /locus_tag="VHT22_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEX3136007.1"
                     /translation="MLPVSMPVAAAAPAAEPSPQEVVEKADEVRFPKESFQVEVQIVS
                     KTGDSEPEGRRYRILAKGNEAAIVLTVEPESERGQALLMRGRDLWVFMPDVSQPIRLS
                     LSQRLVGQVANGDVARANFSGDYTAKVLRYDPCGDEACYVLELEANDSAVAYHRVVYW
                     VEQKTFHPFKAEFYAISGRLLKTATYSAFKPLGGKIRPTEVLIEDAVKKGQSLLSYSG
                     LRLRDLPDKVFNKDYLKRL"
     gene            2305..>2633
                     /locus_tag="VHT22_00265"
     CDS             2305..>2633
                     /locus_tag="VHT22_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3136008.1"
                     /translation="MVVRVEQVTKQYSLDGQRVTALSGVSVNIEEGVFLAIAGPSGSG
                     KSTLLNLIGCIDTPTSGGVWIDGEDVAKRTPDQLAGLRARAIGFVFQSFNLFPVLTAA
                     ENVEYPLL"
BASE COUNT          451 a          856 c          830 g          496 t
ORIGIN      
        1 atcggctgcg ccgccgcggc aatcgcatcg ctgccccctg cggcgcgcgc atcccgcctg
       61 cgggtcgtgg acgcgcttcg cgtgactcac tgacgtgccc cgccgtcccg acaacaggcg
      121 tttacagccc gattggagag cgctcggcgc gctgctcaag ctcgcggcgc agaacgtgct
      181 gcggcagcgc ggccgcagcg cgacggcgct gatcgcgatc gcgctcggcg tcgcgtcgct
      241 gatcgttgcc ggtggattca tccacgacgt ctatatccag atgggcgagt cgatcattca
      301 ctcgcagtac ggtcatcttc agatctacag gcgcgggtac gttcaacacg gaacgcagca
      361 tccgaccgac tatctgatcg atgatcctga tgcggtaatc cagcgcattc aggctttgcc
      421 ccgtgttgca atcgtgctgc cccgattgca tttcaccggc ctggctaacg ccggcggcgc
      481 cgactgggcg atcgtcggag aaggcatcga gccgaagctc gaaaatcagc ttggcaccta
      541 cgtccagctc gtcagcggcc ggctgctgcg cgaggacgat cgcgatgccg tcatcgtcgg
      601 cgatggcgtg gcaaaggccc ttcgattgtc gagcggtcaa ccgataacgt tgagcataag
      661 cacccgcgcg ggggcattga acaccgtcga ttttcaagtg gttggaatct ttcgctccta
      721 ttcgaaggaa ttcgaccagc gctcgatccg catgccgctg aaggcagcca aggatctgct
      781 ggccacgagc gcggtcaacg aaattgccgt tgcgctcacc gacacggcgg caaccgatgc
      841 aactgcctcg ctggcagacg cgagcatcgg ccagctcgga tacgaggttc gcagctggcg
      901 cgacctcgcg gatttctaca acaagacggt tcacctcctc gagcgggaat tcgcctttct
      961 cgtgggcgtg atgctgctga tgatcgtgct gtccgtcgcc aacacgatca acactgcggc
     1021 gttcgaacgc cttcccgaat tcggcacgat gctggcgctc ggcgacaggc gtcgcgatgt
     1081 ttttcgcctg atcatgctcg agtgcacgat cctcgggatg ctgggcagcc tgctcgggct
     1141 cggcatcggg gtcgcgcttg ctcttgcctt gtccgcgctc ggtatttcga tgccgccgcc
     1201 gccgaacacg gacctcggct ataccgcgct gatacggatc gtgcccggcg tgattttcgg
     1261 cgccgtggcg gtgggagtcg ttgccgcgat gctggcgggt gtctttccgt cgctgcgcat
     1321 tgcgcgaacc gcgccggcgg aggcgctgcg gatggcgctg tgaggcccgc gaagcggatg
     1381 ggcggcgccc tagagccgct ttaaataatc cttgttgaac accttgtcgg gcagatcccg
     1441 gaggcgaagg cctgaatacg acagcagcga ttgccccttc ttgaccgcgt cctcgatcaa
     1501 cacctcggtc ggccggatct tcccgcccaa tggcttgaag gcgctgtagg tcgccgtctt
     1561 gagcaggcgt ccggagatcg catagaactc cgccttgaac ggatggaatg tcttttgctc
     1621 gacccagtag acgacgcgat ggtaggccac agccgaatcg ttggcctcga gctccagcac
     1681 atagcaagcc tcgtcgccgc acgggtcgta gcgaaggacc tttgccgtgt agtcgccgct
     1741 gaagttcgca cgggcgacgt cgccgttggc aacctgcccg accagccgct gggacagcga
     1801 caatcgaatc ggctgcgaaa catccggcat gaacacccac aggtcgcgtc cgcgcatcag
     1861 aagcgcctgc ccgcgctcgg attccggttc gacggtcagg acgatcgcgg cttcattgcc
     1921 cttggcgagg atccgatagc gtcggccttc cggctcgctg tcgccggtct tcgagacgat
     1981 ctgcacctcg acctgaaatg actctttggg aaagcgtact tcgtccgcct tttccacgac
     2041 ctcctgaggc gagggctccg ccgccggcgc tgccgcagcg accggcatcg ataccggcag
     2101 catcgcgact gccaaacagc ctatactcca ccggcgtagc aggcggccgg cccggctgcg
     2161 atgagacgcg ctcaacctcg ctccgcgcgc cgctgatgca ttggtgggcc ttggctcggg
     2221 tgacatcgaa tggcaacaac tccaaccgtc gaacggcccg attatagggc gcacgcagcc
     2281 gcgacgcccg ccgtcccggc ggcgatggtg gtccgcgtcg agcaggtcac caagcaatac
     2341 agtctcgacg gccaacgggt cactgcgcta agcggcgtca gcgtgaacat cgaagaaggc
     2401 gttttcctgg cgatcgccgg cccgtcgggc agcgggaagt cgacgttgct caacctgatc
     2461 ggctgcatcg atacgccgac ttccggcggt gtctggatcg acggcgaaga cgttgcgaag
     2521 cggactccgg accagctcgc gggtctgcgg gcgcgcgcca tcggattcgt ctttcagagc
     2581 ttcaacctgt ttccggtcct gacggcggcg gaaaatgtcg agtacccgct gct
//
LOCUS       DATANS010000019         1918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_505185, whole genome shotgun sequence.
ACCESSION   DATANS010000019 DATANS010000000
VERSION     DATANS010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505185"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VHT22_00270"
     CDS             complement(<1..746)
                     /locus_tag="VHT22_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEX3136009.1"
                     /translation="MAEDWQRHISRIEQLAREKGLNYHRVEFEVVPESFMTEIAIYGL
                     PVRMPHWSFGVRYIYQLIQHRMGLSRLFEVVFPGNPGRAYLAKSNSAAENVLVTAHVL
                     GHADFSANNMLFRRSQDQVGEHIVEQAAAHARQIGMAIEEHGLERVESVLDAALSLEQ
                     HIDIDQGLRRARYPELVAQPAAPPNDDFSRRFAALGPDTARPQASGPAKRAPVPPHPE
                     RDLLWFIAQYAPELEPWERDIFLAVREESF"
     gene            complement(750..>1918)
                     /locus_tag="VHT22_00275"
     CDS             complement(750..>1918)
                     /locus_tag="VHT22_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HEX3136010.1"
                     /translation="GGGGSGGGSGGPGDAGWYDLFSRGARDWLRHNDKVRDAVRQHLP
                     QIVAGADVLNGGATTVRVPVKMLEHYRFKLRNSNEQQGVGQGQAKPGDRIGRPQQDSD
                     NQRGQGGNEDGGIQYTLEFRIDDIVDWLWEEMQLPNLEAKAGRAKEDEWTREGWDRRG
                     ARSRLDRRRSMKESIKRRGMQTAQGQDSPSFTDDDLRYRQLTKREQPAMQAVVILLLD
                     VSGSMGERERQISKTFFFWAIQGLRRQYRHLELVFVAHTSEAWEFQEEEFFRVTGTGG
                     TVASAGLKKVREVIDARFSPSQFNVYLFYASDGENFPSDQPQAQTALEELVDDCNFAG
                     FLEVAPLAGAAPDSEIGRLFVEIARDEANVGAYRVSGTDDIWNAVRHFFGRASE"
BASE COUNT          316 a          632 c          619 g          351 t
ORIGIN      
        1 tagaacgact cttcgcgcac ggcgaggaag atgtcgcgtt cccacggctc cagctccggc
       61 gcgtactggg cgatgaacca caacaggtcg cgctcgggat gcggcggtac aggcgcacgc
      121 ttcgcaggac cgctcgcttg cggacgcgcc gtatcaggac caagcgcagc gaaacgccgc
      181 gagaagtcgt cgttgggcgg tgcggccggt tgcgccacca gctctggata gcgcgcgcgg
      241 cgcagaccct gatcgatatc gatgtgctgt tcgagcgaca gcgctgcgtc gagcactgac
      301 tccacgcgct ccagcccgtg ctcttcgatc gccatgccga tctgccgcgc atgcgccgcg
      361 gcctgctcga cgatgtgctc gccgacctga tcctgactgc gacggaacag catgttgttc
      421 gcggagaagt cggcgtggcc aagcacgtgc gccgtgacca gcacgttctc cgctgcgctg
      481 ttgctcttcg cgagatacgc gcgccccggg ttgccgggga acacgacctc gaacagacgc
      541 gacaggccca tgcggtgctg gatcagctgg tagatgtagc gcacgccgaa cgaccagtgc
      601 ggcatgcgca ccggcagacc gtagatcgcg atctcggtca tgaagctttc gggtacgact
      661 tcgaactcga cgcggtggta attgagcccc ttctcgcgcg cgagctgctc gatgcgggaa
      721 atgtgccgtt gccagtcttc agccatgggt tactcggaag ctcgaccgaa gaagtggcgc
      781 acggcgttcc agatgtcgtc cgtgccgctg acgcggtacg caccaacatt ggcttcatcg
      841 cgtgcgatct cgacgaacag ccggccgatc tcgctgtccg gtgcggcgcc tgcgagcggc
      901 gcgacttcca ggaagccggc gaagttgcaa tcgtcgacca gctcttccag cgcggtctgc
      961 gcctgcggct gatccgacgg gaagttctcg ccatcggagg cgtagaacag atagacgttg
     1021 aactgactcg ggctgaagcg cgcgtcgatg acttcgcgca ctttcttcag gcccgcggac
     1081 gcaaccgtgc cgccggtgcc ggtgacgcgg aagaattcct cttcctggaa ttcccaggcc
     1141 tcggacgtgt gcgcgacgaa caccagttcc aggtgacggt actggcgacg caaaccctgg
     1201 atcgcccaga agaagaacgt cttggagatc tgccgctcgc gctcgcccat gctgcccgag
     1261 acgtcgagca gcaggatcac gactgcctgc atcgcgggtt gctcgcgctt ggtgagctgg
     1321 cgatagcgca gatcgtcgtc ggtgaaggag ggcgagtcct ggccttgtgc agtctgcatg
     1381 ccgcgccgct tgatcgattc tttcatcgag cgccggcgat ccaagcgcga gcgcgcacca
     1441 cgacggtccc agccttcgcg cgtccactca tcttctttgg cgcgaccggc cttcgcttcg
     1501 agattcggca gctgcatctc ttcccacagc caatcgacga tgtcgtcgat gcggaattcg
     1561 agcgtgtact ggatgccgcc gtcttcgttg ccgccctgac cgcgctgatt gtcgctgtcc
     1621 tgttgcggac ggccgatgcg atcgccgggc ttggcctggc cttgcccgac gccttgctgt
     1681 tcgttggagt tgcgcagctt gaagcggtag tgctcgagca tcttgacggg cacgcgcacc
     1741 gtcgtggcgc cgccgttgag cacgtccgcg ccggcgacga tctgcggcaa gtgctgccgg
     1801 acagcgtcgc ggactttgtc gttgtgacgc agccagtcgc gggcaccacg cgagaacaga
     1861 tcgtaccagc ccgcatcgcc cggaccgccg ctaccaccgc cggagccacc tccgcccg
//
LOCUS       DATANS010000020         1951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_294679, whole genome shotgun sequence.
ACCESSION   DATANS010000020 DATANS010000000
VERSION     DATANS010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294679"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VHT22_00280"
     CDS             <1..288
                     /locus_tag="VHT22_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX3136011.1"
                     /translation="AINDTGYGLTLGIHSRIDATVRHIASRVRVGNVYVNRNIIGAVV
                     GVQPFGGEGLSGTGPKAGGPNYLFRFAVERTLSINTAAAGGNAALLAQDEA"
     gene            complement(342..701)
                     /gene="folB"
                     /locus_tag="VHT22_00285"
     CDS             complement(342..701)
                     /gene="folB"
                     /locus_tag="VHT22_00285"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX3136012.1"
                     /translation="MNTIFIADLKVETRIGVYDWEQRLKQPLLLNLEIEPPSAAAFAS
                     DSFADALDYAAVVARVKGFAADHPHKLLERFAAALADLVCEEFGARWVSVSVAKPAPI
                     AGVRQIGVKVERGERSA"
     gene            768..1373
                     /gene="plsY"
                     /locus_tag="VHT22_00290"
     CDS             768..1373
                     /gene="plsY"
                     /locus_tag="VHT22_00290"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX3136013.1"
                     /translation="MPTLGVIVAAYLLGSVSFAVVVSKIFGLPDPHSYGSGNPGATNV
                     LRSGNKAAAVLTLLGDGIKGFVAVWAANRLGASWGDASLATAGAALAVYLGHLYPVFH
                     HFAGGKGVSTAAGVAFALSWQLGLAVTVIWLIVVLAFRMSSFASLVAALALPPLGFYF
                     LGFWPESWALIPIALLAFWRHRANIRKLIAGEERRIGEKSV"
     gene            complement(1406..>1951)
                     /locus_tag="VHT22_00295"
     CDS             complement(1406..>1951)
                     /locus_tag="VHT22_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830909.1"
                     /note="universal genome maintenance protein;
                     Kae1/Qri7/OSGEP/YgjD family protein; in Archaea, some Kae1
                     are found as fusion proteins similar to two distinct
                     proteins in yeast that are involved in the KEOPS complex;
                     kinase associated endopeptidase 1 (Kae1) and a
                     serine/threonine protein kinase (Bud32); in Pyrococcus
                     Kae1 has atypical AP endonuclease activity and inhibits
                     the kinase activity of Bud32; the protein is essential in
                     Escherichia coli and Bacillus subtilis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX3136014.1"
                     /translation="GEAFDKTAKLLGLPYPGGPALSKLAERGRHGIFALPRPMKDSGD
                     LNMSFSGLKTAVRTLARDTTPSVQRDADIAREFEDAITDVLVAKSLAALDAAGLSRLV
                     VAGGVGANRSLRERLSDAVAARGGEVFYPDLQFCTDNGAMIALVGALRLQQGLAQDYA
                     FGVRPRWELSSLARPESRASS"
BASE COUNT          310 a          655 c          646 g          340 t
ORIGIN      
        1 gcgatcaacg acaccggtta cgggctgacc ctgggcatcc acagccgcat cgacgcgacc
       61 gtgcggcaca tcgcatcgcg cgtgcgcgtc ggcaacgtct acgtcaaccg caatatcatc
      121 ggcgccgtcg tcggcgtcca gccgttcggc ggcgaagggc tgtcgggaac tggaccgaag
      181 gccggcgggc ccaactatct cttccggttc gcggtggagc gcacgctgtc gatcaacacc
      241 gctgctgccg gaggcaacgc cgcgctgctg gcgcaagacg aggcatagcg tcgcgcggta
      301 gcggcgcccg tgctatcggg ccgaacctgc cgagcagaac gctaggcgct gcgctcgccg
      361 cgctcgacct tgacgccgat ctgcctgacg ccggcgatcg gcgcaggctt ggcgacgctg
      421 acgctgaccc agcgcgctcc gaactcctcg catacgaggt cggccagcgc ggcggcgaag
      481 cgctcgagca gcttgtgcgg atggtccgcc gcaaaaccct tcactcgcgc aacgaccgcc
      541 gcgtaatcga gcgcgtcggc gaagctgtcg ctggcgaacg ccgcagccga aggcggctcg
      601 atctccagat tgagcagcag cggttgcttc aggcgctgct cccaatcgta gacgccgatt
      661 cgcgtctcga ccttgaggtc ggcaatgaag atcgtgttca tccttagcga cgatatggcg
      721 gtttgctaga atgcgcgcgc attgtaatcg agatggagga ctctcgtatg ccgacgctcg
      781 gcgtgatcgt cgccgcgtat ttgctgggct cggtatcgtt tgccgtcgtc gtcagcaaaa
      841 tattcggtct gcccgatccg cacagctacg gctcgggcaa tccaggagcg accaacgtat
      901 tgcgcagcgg caataaggcg gcggccgtgt tgaccctcct cggcgacggc atcaagggct
      961 ttgtcgcggt atgggccgcc aatcgcctgg gcgcgagctg gggggacgcc tccctcgcga
     1021 ccgcgggcgc cgcgcttgcc gtgtacctcg gtcacctgta tccggtgttc catcatttcg
     1081 ccggcggcaa gggcgtgtcg acggcggcag gcgtcgcgtt cgcactgtcg tggcagctcg
     1141 gtctcgcggt gaccgtcatc tggctgatcg tcgtgctggc gtttcgcatg tcatcgttcg
     1201 cctcgctcgt cgctgcgctg gcgctgccac cgctgggttt ttatttcctc ggtttctggc
     1261 ccgaatcgtg ggcgctgata ccgatcgcgc tgctggcgtt ctggcgccat cgcgccaaca
     1321 tacgtaagct gattgccggc gaagagcgca ggatcggaga gaaatcagtc tagaaggcgc
     1381 gcgcggcgac gtcgcgtacg ccccttcagc tgctggcgcg cgattcaggg cgcgcaaggc
     1441 tcgaaagctc ccagcgcggc cgcacgccga aggcgtagtc ctgcgccaag ccctgctgca
     1501 gccgcagcgc gccgaccagc gcgatcatcg cgccattgtc cgtgcagaac tgcagatccg
     1561 gatagaagac ttcgccgcct cgcgccgcta ccgcgtcgct caagcgctcg cgcaagctgc
     1621 ggttggcgcc gacgccgccc gccacgacca gccgcgacag gccggcggcg tcgagcgcgg
     1681 ccagcgactt cgccacgagc acgtcggtga tcgcatcttc gaattcgcgc gcgatgtccg
     1741 cgtcgcgctg cacggaaggc gtcgtgtcac gtgcgagcgt gcgaaccgcg gttttcaaac
     1801 cggagaagct catgttgaga tcgccgctgt ccttcatcgg tcgcggcaac gcaaaaatgc
     1861 cgtgccggcc gcgctcggca agcttggaca gcgccggacc gccgggatac ggcagaccga
     1921 ggagcttcgc cgtcttgtcg aaggcctcgc c
//
LOCUS       DATANS010000021         3376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_379002, whole genome shotgun sequence.
ACCESSION   DATANS010000021 DATANS010000000
VERSION     DATANS010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379002"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1513
                     /locus_tag="VHT22_00300"
     CDS             <1..1513
                     /locus_tag="VHT22_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEX3136015.1"
                     /translation="GGVCLSFLPGFRSLSIAPDIFFLLFIPPLLFADAWLIPKRDLLK
                     VIRPVLLLAFGLVFLTVLVVGYLMHWLVPELPLAAAFALGAVISPTDAVAVRAVTSKL
                     AIPSRVSTIVDGESLINDASGLIAFKFAIAAVMTGAFSWESAALQFFVSSGGGFLLGL
                     AIAWVIGQARVGLRRFCADDPTIQTVISLLTPYAAYLAAEELGIGTILAVVGAGLYAG
                     AHDLRNLDAATRAHSWEVWRMVLFAFNGLVFVLLGVQLHSIIGALSRSSALELAGYAL
                     ALSAIVIVLRILWVFPAAYVPLLLSRRIREREGVHSPRAVFLTGWAGIRGSITLAAAL
                     SIPFVTSSGAPFPGRELIILLAASVIIVTLLVNGMTLPLLIRWLDIRGDRIAEREERA
                     ARIATAQAAIESLRKRLPRLTTSEETAFANSLLGQYEQRLQRYSANAERRSRLDAQHA
                     SERRIWLAAIAAERTELMQMRETDVINDEVLRRLQVDLDLEETRITGAARDAI"
     gene            1565..2239
                     /gene="nadD"
                     /locus_tag="VHT22_00305"
     CDS             1565..2239
                     /gene="nadD"
                     /locus_tag="VHT22_00305"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018881299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX3136016.1"
                     /translation="MSSAIGILGGTFDPIHYGHLRAAAEVRDQLRLDEVHLIPASVPP
                     HRPPPVAPAEARLAMTVLGCAEFPGLVADDREILRPGPSYTVQTLQEMHEENPGRPLA
                     MIIGSDAFCGIASWRRWEQLFTLAHFIVVDRPDRPFALDALEPALKVQCERRLTTDPM
                     RLSRQLAGAVIRLVVTPQPISATALRAALAAGAEARERIRGLLPAAVLAYIERNQLYR
                     SVPDAR"
     gene            2229..2585
                     /gene="rsfS"
                     /locus_tag="VHT22_00310"
     CDS             2229..2585
                     /gene="rsfS"
                     /locus_tag="VHT22_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX3136017.1"
                     /translation="MRANKIQKTAITALEDIKARDITVLDVRKLTSLYDTLIIATAES
                     GRQAGALARHVRDSLKAAGATIVGVEGEASGEWVLVDAGDIVVHIMQPAVRTYYNLEE
                     LWTPPAQRRRARAVAA"
     gene            2608..3075
                     /gene="rlmH"
                     /locus_tag="VHT22_00315"
     CDS             2608..3075
                     /gene="rlmH"
                     /locus_tag="VHT22_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX3136018.1"
                     /translation="MKLRLVALSAKLPAWMTAGYAEYARRMAREFILELVELKPEPRD
                     RGKTTPQILAAEAARIADATGGYHVVALDERGQAWTTGEFAAKLTGWRERALAVAFVI
                     GSADGLDPAVKRNADAVLALSALTLPHGLARVVLAEQLYRATSMLSGHPYHRE"
     gene            3072..>3376
                     /locus_tag="VHT22_00320"
     CDS             3072..>3376
                     /locus_tag="VHT22_00320"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX3136019.1"
                     /translation="MSTLFMLTERPSVYLASKSPRRHELLRQIGVEFEELLLREAPGR
                     RRDIVEVPRKDEAPFEYVKRVARTKAAVGWHRMGRRELLPKPVLAADTEVVVDGIVLG
                     "
BASE COUNT          516 a         1104 c         1138 g          618 t
ORIGIN      
        1 cggcggtgtg tgcctgtcgt tcctgccggg tttccgctcg ctctcgatcg cgccggacat
       61 cttttttctg ttgttcattc cgccactgct gttcgccgac gcttggttga tacctaagcg
      121 cgacctgctc aaggtgatcc ggccggtgct gctgctcgcc ttcgggctag tgtttctaac
      181 ggttctggtc gtcggctatt tgatgcattg gctggttccc gagctcccgc tcgcggcggc
      241 attcgcgctc ggcgcggtga tctcgcctac cgatgcggtc gcggtcaggg cggtaacctc
      301 caagctcgcg attccctcgc gcgtctcgac gattgtcgac ggcgaaagcc ttatcaacga
      361 cgcatcgggt ttgatcgcgt tcaagttcgc gatcgcggca gtgatgaccg gcgccttcag
      421 ctgggagagc gcagcgctgc agtttttcgt ctcttccggc ggcggtttcc tgctggggct
      481 tgcgattgcc tgggtgatag gccaggcgag ggtaggcttg cgccggttct gcgccgatga
      541 cccgacgatc cagacggtga tctcgctgct gacgccctat gcggcgtatc tcgccgcgga
      601 ggagctgggc atcggcacga tcctggccgt cgtcggcgcg ggactctacg ccggcgcgca
      661 cgacctcagg aacctggacg ctgcgacgcg cgcgcattcg tgggaagtat ggcggatggt
      721 gctgttcgcg ttcaacggcc tggtgttcgt gctactgggc gttcagctcc attcgatcat
      781 cggcgcgctt tcgcgctcga gcgcgctcga gcttgccggc tacgcgctgg cgctatcggc
      841 cattgtgatc gtgctgcgca tcctctgggt ctttccggcc gcatacgtgc cgttgctcct
      901 ttcgcggcgg attcgcgaac gcgaaggcgt gcacagccct cgtgcagtgt tcctgaccgg
      961 atgggcgggc attcgcggct cgatcacgct tgccgccgcg ctttcgattc cgttcgtgac
     1021 ctcgagcggc gcgccgtttc cggggcgcga gctgatcatt ctgcttgccg cgagcgtgat
     1081 catcgtcacg cttctggtca acggcatgac gcttccgctg ctgattcgct ggctcgatat
     1141 ccgcggcgat cgtatcgccg agcgcgaaga gcgcgccgcc cgcatcgcga ccgcgcaggc
     1201 ggcgatcgag agcttgcgca agcggctgcc gcgtctgacg accagcgagg agaccgcgtt
     1261 cgcgaattca ctcctagggc agtacgagca gcggctgcag cgctattcgg ccaatgcgga
     1321 gagacggagc cggctcgacg cccagcacgc gtcggagcgc cggatctggc tggcggcgat
     1381 cgccgccgag cgcaccgagc tgatgcagat gcgcgaaacc gacgtgatca acgatgaagt
     1441 gctgcgtcgc ctccaggtcg atctggacct cgaggaaacg cgcatcaccg gcgccgcgcg
     1501 cgatgcgatc tgaacgcggg ctcgcgcgcg acggcggcac ccgaagcgcg gaagcgtgcc
     1561 cgcaatgagc agcgcgatcg gcatcctggg ggggactttc gatccgattc actatgggca
     1621 tcttcgcgcc gcggcggagg tgcgggacca gctgcgtctg gacgaggtgc atttgattcc
     1681 cgccagcgtt ccgccgcacc ggcctcctcc ggtcgccccg gccgaagcgc ggctggcgat
     1741 gaccgtgctg ggctgcgcgg agtttcccgg cctcgttgcc gacgatcgcg agatcctgcg
     1801 tccggggccg agctacaccg tgcagacctt gcaggaaatg cacgaagaga acccgggccg
     1861 gccgctggcg atgatcatcg gcagcgacgc gttctgcggc atcgcgagct ggcggcgctg
     1921 ggagcagctt ttcacgctcg cgcatttcat cgtcgtcgac cggccggaca gacctttcgc
     1981 gctggacgca ctggaaccgg cgctgaaagt ccagtgcgag aggcgactga ccaccgatcc
     2041 gatgcggctg tcgcggcaac tcgccggcgc agttatccgg ctggtggtga caccgcagcc
     2101 gatttcggcg acggcgctgc gcgcggcgct cgccgccgga gccgaagcac gggagcgaat
     2161 ccgcggtttg cttcccgccg cggttctggc ttatattgaa cgcaaccaac tctatcgatc
     2221 cgttccggat gcgcgctaac aagattcaga agaccgcaat cactgccctc gaagacatca
     2281 aagcccgcga cattaccgtg ctagatgtcc gcaagctcac cagcctctac gacacgctga
     2341 tcattgccac cgccgaatcg ggccggcaag ccggcgcgct ggcacgccac gtccgcgatt
     2401 cgctgaaggc ggcaggtgcg acgatcgtcg gcgtcgaagg cgaagcgtcg ggcgaatggg
     2461 tgctggtcga tgccggcgac atcgtcgtgc acatcatgca gccggcggtg cggacctact
     2521 acaatctcga agagctctgg acgccgccgg cgcagcggcg ccgcgcccgc gcagtcgcag
     2581 catagcgcgg cggcatccgg gagcgcgatg aagcttcgtc tcgtcgcgct gagtgccaag
     2641 cttcccgcct ggatgaccgc ggggtatgcc gaatatgcgc gccgcatggc gcgcgaattc
     2701 atcctggagc tcgtcgagct caagcccgag ccgcgcgatc gtggcaaaac cacgccgcag
     2761 attctcgccg ccgaagcggc tcggatcgcc gacgcgaccg gtggctatca tgtcgttgca
     2821 ctcgacgagc gcggccaagc atggacgact ggagaattcg ccgcgaagct caccggctgg
     2881 cgcgagcgcg cgctggcggt ggcgttcgtc atcggcagcg cggacgggct cgatcccgcc
     2941 gtcaagcgca acgcggatgc cgtgctggcg ctttccgcgc taacattgcc gcacgggctt
     3001 gcacgggtcg tgctcgccga acagctgtat cgcgcaacga gcatgctgtc gggacatccg
     3061 taccatcgcg aatgagcacg ctcttcatgc tgaccgagcg cccgtcggtc tatctggctt
     3121 cgaaaagccc gcgccggcac gagttgcttc ggcagatcgg tgtcgaattc gaggagctgt
     3181 tgctgcgcga ggcgccgggg cgccggcgcg acatcgtcga ggtcccgcgc aaggacgaag
     3241 ctccattcga atacgtcaag cgcgtcgcgc gcaccaaggc cgccgtcggc tggcatcgca
     3301 tggggcgccg cgagttgctg ccgaagccgg tgctcgcggc ggataccgaa gtcgtcgtcg
     3361 acggcatcgt gctggg
//
LOCUS       DATANS010000022         4496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_379083, whole genome shotgun sequence.
ACCESSION   DATANS010000022 DATANS010000000
VERSION     DATANS010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379083"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VHT22_00325"
     CDS             complement(<1..835)
                     /locus_tag="VHT22_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEX3136020.1"
                     /translation="MLRRIARGGIAVALDTATRSAIRASRLAVQQAVDHGAPAYGINT
                     GFGKLAKTHIAHDQLERLQTNLVHSHAVGTGPLLDDATVRLVLVLKAASLARGYSGVR
                     PEVIDALMALYNAGVLPSIPSQGSVGASGDLAPLAHMTLALMGEGEVRIDGAAMAASE
                     GLRRAGLSAMSLAAKEGLALLNGTQVSTALALKALFEVEDIFAAAVVTGALSVAAARG
                     SDTPFDARIQEVRGQPGQIDVAAKYRGLLSGSEIRRSHLVDDDRVQDPYSLRCQPQVM
                     GA"
     gene            complement(968..2686)
                     /gene="hutU"
                     /locus_tag="VHT22_00330"
     CDS             complement(968..2686)
                     /gene="hutU"
                     /locus_tag="VHT22_00330"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX3136021.1"
                     /translation="MNDLSPPRPSNTRVDPTRRIRAARGTRLSAKSWLTEAPLRMLMN
                     NLDPEVAEKPDELIVYGGIARAARDWQCFDKIVEVLKELDDDQTLLVQSGKPVGVFKT
                     HADAPRVLIANSNLVPHWATWEHFNELDAKGLMMYGQMTAGSWIYIGSQGIVQGTYET
                     FAEAGRQHYGGKLDGRWILTGGLGGMGGAQPLAATMAGASMLAVECNPSRIQMRLDTG
                     YLDQQAATLDRALAIIQDAHAGGRAVSVGLLGNAAEVFPELVRRVQGGDRKARPDMVT
                     DQTSAHDPLNGYLPSGWSLEEAASRRAGDPAGVVKAAKASMAVQVRAMLAFHQMDVPT
                     FDYGNNLRQMALEAGVGSAFDFPGFVPAYIRPLFCRGIGPFRWVALSGDPEDIYRTDA
                     KLKQLLPQNAQLHHWLDMARKRIKFQGLPARICWIGLGDRARAGLAFNDMVASGELKA
                     PIVIGRDHLDSGSVASPNRETEGMRDGSDAVSDWPFLNALLNCASGATWVSIHHGGGV
                     GMGFSQHAGMVIVCDGTPAAARRIERVLTNDPASGVMRHADAGYEDAIQCATEQGLVL
                     PMVDRK"
     gene            2859..3047
                     /locus_tag="VHT22_00335"
     CDS             2859..3047
                     /locus_tag="VHT22_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019585678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transport regulator"
                     /protein_id="HEX3136022.1"
                     /translation="MMPYNTVADLPKAQTDQYNPHQKEAFLKAFNSAYREYGGDEGRA
                     FAVAHTAAKRAGEKPGPG"
     gene            3406..>4496
                     /locus_tag="VHT22_00340"
     CDS             3406..>4496
                     /locus_tag="VHT22_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017286316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX3136023.1"
                     /translation="MIQPFAPASTPDQEQLRIVPAKRYSGKVHLLPATMETTQWGWFN
                     NAQPPVLHVDSGDSIVFETMMHSHNQVVPGTTIEQIKKLRTDFPGRGPHTLTGPVYIE
                     GAEPGDVLKVRINRIVPRAYGTNFNVPGMFGEFPSKFPEGQVKYFYLDLERKVAEFAP
                     GIEIPLAPFPGTIGVARAEPGQYSSVPPGRYAGNIDVRDLKEGATLYVPVFVKGALLW
                     TGDSHAAQGNGEINLTALETAYKEMNVTVDVVKNMKLEWPRIETKDAWITLGIDRDLN
                     KALDILMGETSKLLIEQRSLDNGQAQKMMMATWDCRISQVVDVNKGIHCFSAKGAKAA
                     RKIEALPDQEDKTYLVTVGKDADLNKAMD"
BASE COUNT          802 a         1523 c         1451 g          720 t
ORIGIN      
        1 aagcgcccat gacctgcggt tggcagcgca ggctgtaagg atcctgcacc cgatcgtcgt
       61 cgaccagatg cgagcggcga atctcgctgc cgctaagcag gccacgatac tttgccgcga
      121 cgtcgatttg tcccggctgg ccgcgcactt cctggatgcg cgcgtcgaac ggcgtgtcgc
      181 tgcccctggc ggcggcgacc gacaacgcac ccgtcacgac cgcggcagcg aaaatatcct
      241 cgacctcgaa cagcgccttg agcgccagcg ccgtcgatac ctgcgtgccg ttcagcagcg
      301 ccagcccctc cttggcggcg agcgacatcg cagagaggcc agcgcggcgc aagccttcgc
      361 ttgccgccat cgcggcgccg tcgatgcgca cttccccctc gcccatcagc gccagcgtca
      421 tgtgcgccag cggcgcaaga tcgccggagg ctccaaccga tccttgcgaa ggaatcgacg
      481 gcaggacgcc ggcgttataa agcgccatca acgcgtcgat cacttcgggc cggacgccgg
      541 aatacccgcg cgccaggctc gcggccttga gcaccagcac gagacgcacg gtcgcatcgt
      601 cgagcaaagg gccggtgccg acggcgtgcg agtgaacgag gttggtctgc agccgctcca
      661 gctgatcgtg cgcaatgtgc gtcttggcga gcttgccgaa gccggtgttg atgccgtagg
      721 cgggggcgcc gtgatcgacc gcctgctgca ccgccaatcg cgaagcgcgg atggccgatc
      781 gcgtcgcggt gtcgagggcc acggctatgc cgccgcgcgc gatccggcga agcatgccga
      841 gcgcaagccc gcccgcggca atcgtgagcc cggccttcat caggccggcg tccgggtcgg
      901 catgcgcgcg tcaggctcga tggcgaaaag cgccgtcaat cgtgcgcgat cgccatgaga
      961 cccacggtca tttccggtcg accatcggca ggaccagccc ctgctcggtc gcgcactgga
     1021 tcgcgtcctc gtacccagca tcggcgtgcc gcatcacgcc gcttgccggg tcgttggtca
     1081 gcacgcgctc gatgcgccgg gcggcggcag gagtcccgtc gcagacgatg accatgccgg
     1141 cgtgctgcga gaaccccatg ccgacgccgc cgccgtgatg gatgctcacc caggtggccc
     1201 cgctcgcgca attgagcagc gcattgagga aaggccagtc ggacaccgcg tcggatccgt
     1261 cgcgcatgcc ttcggtctcg cgattgggac tcgccaccga ccccgagtcg agatggtcgc
     1321 ggccgatgac gatcggcgcc ttcaactccc cactggcgac catgtcgttg aacgcgaggc
     1381 ccgcgcgcgc ccgatcgccc agaccgatcc agcaaatgcg cgccggcaat ccctggaact
     1441 tgatccgctt ccgcgccatg tcgagccagt gatgcagctg cgcgttttgc ggaagcagct
     1501 gcttcagctt ggcgtcggtc cggtagatgt cctcgggatc gcccgacagc gccacccagc
     1561 ggaacggccc gatgccgcgg cagaacagcg ggcgaatgta agcgggcacg aagccgggga
     1621 aatcgaaggc actgccgacg cccgcctcca gggccatctg ccgcaagtta ttgccgtagt
     1681 cgaacgtcgg cacgtccatc tggtgaaagg cgagcatggc gcgcacttgc acggccatcg
     1741 acgccttcgc ggccttcacc acgccggccg gatcgccggc gcgccggctc gctgcctctt
     1801 cgagcgacca accggacggc agataaccgt tgagtggatc gtgcgcgctg gtctggtcgg
     1861 tcaccatgtc agggcgcgct ttgcgatcgc cgccctgcac gcggcggacg agctcgggaa
     1921 aaacctcggc ggcattgccg agcaatccga ccgagaccgc gcggccgccg gcgtgcgcgt
     1981 cctggatgat ggccagcgcg cgatcgagcg tcgccgcctg ctggtcgaga tagccggtat
     2041 cgagccgcat ttggatccgg ctcggattgc attcaaccgc gagcatcgac gctccggcca
     2101 tcgtcgccgc cagcggctgt gcgccaccca tgccgcccag gccgccggtg agaatccatc
     2161 gtccgtcgag cttgcctccg taatgctggc ggccggcttc ggcgaaggtt tcgtaagtgc
     2221 cctgcacgat gccctggctg ccgatataga tccacgaccc ggccgtcatc tgtccgtaca
     2281 tcatcaggcc tttggcgtcg agctcgttga agtgctccca cgtggcccaa tgcggcacca
     2341 ggttggaatt ggcgatcagc acgcgcggcg cgtcggcgtg cgtcttgaac acgccaaccg
     2401 gcttgcccga ctggacgagc agcgtctggt cgtcgtcgag ttcctttaat acttcgacga
     2461 tcttgtcgaa acactgccag tcgcgggcgg cgcgggcgat gccgccgtac acgatcagct
     2521 cgtcgggctt ttccgcgacc tcggggtcga ggttgttcat cagcatgcgc agcggcgcct
     2581 cggtcagcca cgacttggcg ctgagccggg tgcctcgcgc agcgcgtatg cggcgcgtcg
     2641 ggtcgacgcg cgtgttgctg gggcggggag gcgaaagatc gttcatggcg tatccctgac
     2701 tttagcggcg ctcgagatac aaaggtcgat gttatcgccg tggctagcct tatgcaaaaa
     2761 ggcgacgcgc cggccgctgg tcgggaactc gcgccaccgt tcgcacatcc cacgatcggg
     2821 ggcttgcatc gctcgtcaat ccatccgtca aggagaccat gatgccttac aacaccgtcg
     2881 cggatctgcc gaaggcccag accgaccagt acaacccgca tcagaaggaa gcattcctca
     2941 aggcgttcaa cagcgcctac agggaatacg gcggcgacga gggccgcgct ttcgccgtcg
     3001 cccacacggc ggcaaagcgc gccggagaaa agccggggcc gggttagccc actagcctcg
     3061 ggcatcggtg acctgcgtca atgcaaatcg ccggccacgt cggccgatgc tcgagtcgaa
     3121 aaggcggtcg tccaaactgc tagaatcacc gcggagccgt tcccgcatgg gtcgccgtgc
     3181 gtcggccgac taggggaggt tgcgatgacc aaagagactt gtgctgcgcg cgttcgcgcc
     3241 ggcgtgtgcg ttatcggcgg atcggtgatc tggttggcgg tctccgtcac cgcagcgcaa
     3301 caacctgcgg ctccggcgcc cgcggcaccc gctgcacccc cggctgcggc gcagcccgcg
     3361 gcgcctgcgg ccgcagccca acccgccccc actccgcccg cgcctgtgat ccagcccttc
     3421 gctccggcct cgacgccgga ccaggagcag ttgcggatcg tcccggccaa gcggtactcc
     3481 ggaaaagttc atctgctgcc ggcgacgatg gaaacgacgc agtggggctg gttcaataac
     3541 gcgcagccac ccgtgctgca cgtcgattcg ggcgattcga tcgtcttcga gacgatgatg
     3601 cattcgcaca atcaggtcgt gcccgggacg accatcgagc agatcaagaa actccggacc
     3661 gacttcccgg gtcgcggccc ccacacgttg accggcccgg tgtacatcga aggcgccgag
     3721 ccgggcgatg tcctgaaggt gcgcatcaac aggatcgtcc cgcgcgctta cggcacgaac
     3781 ttcaacgtgc ccggcatgtt cggcgagttt ccttccaagt ttcccgaggg ccaggtcaaa
     3841 tatttctacc tcgatctcga gcgcaaggtc gccgagttcg cgccgggcat cgagattccg
     3901 ctggcgccgt tccccggcac gatcggcgtt gcccgcgccg agcccggtca atacagctcg
     3961 gtgccgccgg gacgctacgc cggcaacatc gacgtccgcg acctgaagga aggcgcaaca
     4021 ctctatgtgc ctgtcttcgt caaaggcgcg ctgctctgga ccggcgattc gcatgcggca
     4081 cagggcaacg gcgagatcaa cctgaccgcg ctcgagaccg cgtacaagga aatgaacgtc
     4141 accgtcgacg tcgtcaagaa catgaaactc gaatggccgc gcatcgaaac caaggatgcg
     4201 tggatcaccc ttggcatcga tcgcgatctg aacaaggcgc tcgacatcct gatgggcgaa
     4261 acgagcaaat tgctgatcga gcagcgatcg ctggacaacg ggcaggcgca gaagatgatg
     4321 atggcgacgt gggactgccg gatcagccag gtcgtcgacg tcaacaaggg catccactgc
     4381 ttcagcgcca agggtgccaa ggctgcgcgc aagatcgagg cgctgccgga tcaggaagac
     4441 aagacttacc tcgtgaccgt cggcaaggat gccgacctca acaaggcgat ggacga
//
LOCUS       DATANS010000023         1725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_379140, whole genome shotgun sequence.
ACCESSION   DATANS010000023 DATANS010000000
VERSION     DATANS010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379140"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..829)
                     /gene="paaC"
                     /locus_tag="VHT22_00345"
     CDS             complement(44..829)
                     /gene="paaC"
                     /locus_tag="VHT22_00345"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEX3136024.1"
                     /translation="MNDQRTLFDYLLRRADSDLVLAQRLGEWVGHGPVLEEDIATTNV
                     GLDLLGQSRLWFAYAGEVEARFNNGAGRSEDQLAFLRDAAEFRNLMLVEQPNGDYAVT
                     MARQFYFDVWHELSLRGLTASADVRIAEIAAKALKEVRYHIERSADWVIRLGDGTEES
                     HRRMQAAVDDLWMYTGEMFEVDAIELALIDAGVACDARALAAPWRQRVDAIFSEATLT
                     VPAGVWMQGSGGRGGKQGVHTEHIGPMLAQMQWLQRAYPGAQW"
     gene            complement(832..1113)
                     /gene="paaB"
                     /locus_tag="VHT22_00350"
     CDS             complement(832..1113)
                     /gene="paaB"
                     /locus_tag="VHT22_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HEX3136025.1"
                     /translation="MNEWPLWEVFIRSQHGLAHKHVGSLHAPDAEMAIKNARDVYTRR
                     NEGVSIWAVRSSDIVASSPGDKEPMFEPASSTVYRHPTFFPMPDEVKHI"
     gene            complement(1143..>1725)
                     /gene="paaA"
                     /locus_tag="VHT22_00355"
     CDS             complement(1143..>1725)
                     /gene="paaA"
                     /locus_tag="VHT22_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEX3136026.1"
                     /translation="GAIGWLVDGAAIMNQIPLCRCSFGPYSRAMIRVCKEESFHQRQG
                     FDIMIKLCRGTPEQKAMAQDALDRWWWPSLMMFGPPDASSVHSAQSAQWKIKILSNDE
                     LRQRFIDQTVPQADFLGIRMPDPDLKWNAERGHYDFGAIDWNELYEVVKGNGPCNRER
                     LRARVDAWNDGAWVREAALAHAEKRAAREAKAA"
BASE COUNT          271 a          591 c          531 g          332 t
ORIGIN      
        1 gcgtccatcg ccggacgggc ttcggtcgcg ccgcgaatcg cgttcaccat tgcgcccccg
       61 ggtaggcacg ctgcagccat tgcatctgcg cgagcatcgg gccgatgtgc tcggtatgca
      121 cgccctgctt tccgccgcgg ccgccgcttc cctgcatcca gacgcctgct ggcaccgtca
      181 gcgtggcttc ggagaagatc gcgtcgacgc gctgccgcca cggcgcggcc agcgcgcggg
      241 cgtcgcaggc gacgccggca tcgatcagcg cgagctcgat cgcgtcgact tcgaacatct
      301 cgccggtgta catccacaga tcgtcgaccg ccgcctgcat cctgcgatgg ctctcttcgg
      361 tgccgtcgcc caggcggatc acccagtcgg cgctgcgctc aatgtgatag cgcacttcct
      421 tcagtgcctt ggcggcgatt tcggcgatgc gcacatcggc tgaggccgtc aggccgcgca
      481 gcgacagttc gtgccagacg tcgaagtaga actgccgcgc catcgttacc gcatagtccc
      541 cgttaggttg ctcgaccagc atcaggttgc ggaattcagc ggcatcgcgc aggaacgcga
      601 gctggtcttc gcttcgtccg gccccgttgt tgaaacgcgc ttcgacttcg cccgcgtagg
      661 cgaaccagag gcgcgactgg ccgagcagat cgaggccaac attcgtcgtc gcgatgtctt
      721 cctccagcac cggcccatgg ccgacccatt caccaaggcg ctgcgccagg accaggtcgc
      781 tgtcggcacg ccgcagcaga tagtcgaaca gcgtccgctg gtcgttcatg ttcagatgtg
      841 cttgacttcg tcgggcatcg gaaagaacgt cggatggcga tacaccgtgc tcgacgccgg
      901 ctcgaacatc ggttccttgt cgccgggcga gctggcgacg atgtccgagg acctcaccgc
      961 ccagatgctg acgccttcgt tgcgccgcgt gtaaacgtcg cgcgcattct tgatcgccat
     1021 ttcggcgtcg ggcgcgtgca ggctgccgac gtgcttgtgc gcgagaccgt gctggctccg
     1081 gatgaagact tcccacaacg gccattcgtt cattccggta acccctttcg atgtagattc
     1141 catcacgccg ccttcgcttc gcgcgccgcg cgcttttcgg catgggcgag tgccgcctcg
     1201 cgcacccacg ctccgtcgtt ccaggcgtcg acgcgggccc gcagtcgctc gcggttgcac
     1261 gggccattgc ccttgacgac ttcgtacagt tcgttccagt caattgcgcc gaaatcgtag
     1321 tggccgcgtt cggcgttcca tttcaggtcg ggatcgggca tgcgaatacc gagaaagtcc
     1381 gcctgcggta ccgtctggtc gatgaagcgc tggcgcagct cgtcgttcga caggatcttg
     1441 atcttccatt gcgccgactg cgcgctgtgc accgagctcg cgtcaggcgg accgaacatc
     1501 atcagtgacg gccaccacca gcgatcgagc gcgtcctgcg ccatcgcctt ctgctcgggc
     1561 gttccccggc agagcttgat catgatgtcg aaaccctgcc gctggtgaaa gctctcttcc
     1621 ttgcagacgc ggatcatcgc gcgcgaatac gggccgaacg agcagcggca aagcggaatc
     1681 tggttcatga tcgccgcgcc gtccacgagc cagccgatcg cgccg
//
LOCUS       DATANS010000024         1851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_547310, whole genome shotgun sequence.
ACCESSION   DATANS010000024 DATANS010000000
VERSION     DATANS010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547310"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VHT22_00360"
     CDS             <1..331
                     /locus_tag="VHT22_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008847530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX3136027.1"
                     /translation="PPPDLIKIDVEGAEYRVLKGAEKSIGRGRPAIISEFSLEMTGRV
                     SGVPGAQYLEWIASLQYDIFLLDRITSQLVGIPSLPSFLSTWGSLIRIEDLLFMPHEK
                     AHLLESN"
     gene            426..1565
                     /locus_tag="VHT22_00365"
     CDS             426..1565
                     /locus_tag="VHT22_00365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014002170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3136028.1"
                     /translation="MRIALIHDWLDTWGGGENVLAALLALYPEADLFALVDFLSEANR
                     AKLGHRPIRTSFIQRLPFARRHFRKYFALMPHAVEQFDLSPYELVISSSHSVAKGVRT
                     GPGQLHICLCYSPARYAWDLQPQYLAQTGLDRGVLGWLARLQLARFRRWDVRASARVD
                     RFVAISEYIAQRIRNCYRRDADVIYPPVDVPAVKVDGSPRESFYLTVSRLVPYKRIDL
                     LVQAFSALPGRQLVVAGDGPEMKKLARLAGPNVRLLGVVDDAHRDRLLRTAKAFVFAA
                     DEDFGIAPLEAQAQGTPVIAFGRGASAETIRGLDAAAPTGVLFAEQTIASIGAAVAQF
                     EASAQRITAAACRANAERFSRQTFNRRFSAYVTDALAQHRSRPHP"
     gene            1573..>1851
                     /locus_tag="VHT22_00370"
     CDS             1573..>1851
                     /locus_tag="VHT22_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEX3136029.1"
                     /translation="MLAPRNLLKSNATTFDRLLRLVDPLVIVIVGWASYLIYLGSAVL
                     PPNYLVALLGAALLGVVVFPETGLYRPKRGASLADDLRALTYGWSVVVA"
BASE COUNT          299 a          615 c          574 g          363 t
ORIGIN      
        1 gccgccgccg gacctaatca agatagacgt agaaggcgcg gaatacaggg tgctgaaagg
       61 ggcggagaag tcgatcggcc gcggccgccc agcgatcatc agcgaattca gcctggagat
      121 gacgggccgg gtctccgggg tgcccggagc gcaatatctg gaatggatcg cctcgcttca
      181 gtacgacata ttcctgctcg accggataac gtcgcagctg gtcggcattc cgtcgctgcc
      241 atcgtttctc tcgacgtggg gaagtctcat ccggatcgag gacctgctgt tcatgcctca
      301 cgaaaaggcg cacctgctcg agagcaattg agcgccgatc gtagacggcg agaacaactg
      361 gccgccacac ggtactgctc ccgccgcctt gacccaatgg cagctctgct ccgggcattt
      421 atccaatgcg catcgccctg atccacgact ggctcgacac ctggggcggc ggcgagaatg
      481 tgcttgcggc gctgctcgcg ctctacccgg aagcggatct tttcgcgctg gtggatttcc
      541 tgtccgaagc gaatcgggcg aaactgggac atcggccgat ccgcacttcg ttcatccagc
      601 gtctcccgtt tgcgcggcgg cacttccgga agtattttgc gctgatgccg cacgctgtcg
      661 aacaattcga tttgtcgccc tatgagctgg tgatctccag ctcgcattcg gtcgccaagg
      721 gcgttcgcac cgggcccggc cagttgcaca tctgcctctg ctattctccg gcgcgatatg
      781 cttgggacct gcagcctcaa tatctggcgc agacagggct cgaccgggga gtgctcggct
      841 ggctcgcgcg ccttcagctg gcccgattcc gccgctggga cgtacgtgcg tcggcccgcg
      901 tcgaccgctt tgtcgcgatc tccgaataca ttgcccagcg catacgcaac tgctatcgcc
      961 gcgacgccga tgtcatctat ccaccagtcg acgttcccgc ggtcaaggtc gacggctccc
     1021 cgcgagaatc gttctatctg accgtgtcgc gcctggtgcc atataagcgg atcgacttgc
     1081 tggtgcaggc tttcagcgcg ctcccggggc gacaattggt cgtggccggc gacgggccgg
     1141 agatgaaaaa gctggcgagg ctcgccggtc ccaacgtgcg gctcttgggc gttgtcgacg
     1201 acgcgcaccg cgatcggctg ttgcgcacgg caaaagcctt cgtctttgcc gccgacgagg
     1261 acttcggcat cgcgccgctc gaggcgcagg ctcagggaac accggtgatc gcattcgggc
     1321 gtggcgccag cgccgagacc atacgcggcc tcgatgccgc ggcgccgacc ggcgtgctgt
     1381 ttgccgaaca gacgatcgcc tcgatcggag ccgcggttgc gcaattcgaa gcctccgcgc
     1441 agcgcataac ggccgcggcc tgccgcgcca atgccgagcg cttctcgcgc cagactttca
     1501 accggcgctt ctcggcgtat gtgaccgatg ccctggcgca gcatcgttcc cgaccgcacc
     1561 cctgaccctg cgatgctcgc tccgcgcaat ctgctcaaga gcaacgccac gacgttcgat
     1621 cgactgctgc gcctcgtcga tccattggtt attgtcatcg tcgggtgggc cagttacctg
     1681 atttacctcg gcagcgcagt cctgccgccc aattacttgg tcgccttgct aggcgccgcg
     1741 ctgctcggcg tcgtcgtctt tcccgagacc ggactgtatc ggccgaagcg cggcgcgagc
     1801 ctcgccgatg acctgcgcgc gctcacttat ggctggtcgg tcgtggtcgc g
//
LOCUS       DATANS010000025         2316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_253231, whole genome shotgun sequence.
ACCESSION   DATANS010000025 DATANS010000000
VERSION     DATANS010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253231"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VHT22_00375"
     CDS             complement(<1..969)
                     /locus_tag="VHT22_00375"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEX3136030.1"
                     /translation="MTWLGLLIGGIIGSSWGFKSALLGALIGALIGMYLRKSAARSSE
                     SRTAAAQAGEAVSLAPSPDAAGVATGAMLPVRKGGTGGRFVQAAAEAALATASAAPAV
                     APLTMSAPPAAASVAMSAPAAAPSSDPDPVWAWLTGGNAMTRIGVVILFFGVAFLLRY
                     LAEIVTVPIEWRLVGVAIIGVGLIAIGILLASARPGYGLSLQGAGAGILYLTTFAAFR
                     LYHVLPPVPGLLLLALVAASTVALAIRADSQPLAALAVAGGFLAPILTSVTGEPALLF
                     GYFAILNAAIFALAWVRSWRALNLLGFVFTFVLGSIWAYRYYRPLYF"
     gene            complement(994..>2316)
                     /locus_tag="VHT22_00380"
     CDS             complement(994..>2316)
                     /locus_tag="VHT22_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX3136031.1"
                     /translation="AIAGYLVSRRIPETPAVAPELRINWNPFTETWRNLRFAHGNRIV
                     WLAMLGISWFWFYGATYLTQFANFTKDVLGGDEHVATLLLAIFSIGIGVGSLLCERLS
                     GHKVEIGLVPFGSIGLSVFAIDLYLASRNLAPQGLAGIGAFFAVGAHWRVIADLILIG
                     MFGGFYIVPLYALIQERSEPAFRSRIIAANNILNAIFMVASAGIALALLKAGLSIPQL
                     LLVTGLMNAAIALYIYLLVPEFLMRFLAWLLVHTFYRVDKEGLERIPPEGPCVIVCNH
                     VSFVDAIVIAACVRRPIRFVMDHRIFRIPLLNFIFRTMRTIPIAPAREDAAMKERAFA
                     EAAAALRAGEIVGIFPEGKLTETGDLNPFRPGLQRILEQAPAPVVPMALRGLWGSFFS
                     RSDRGRAMRKIRGLFSRIALVAGPPVPPERATPEYLQQNVLALRGDWR"
BASE COUNT          473 a          747 c          783 g          313 t
ORIGIN      
        1 gaaatagagc ggccggtaat agcgatacgc ccagatcgag cccaggacga aggtgaagac
       61 aaatccgagc aggttcagtg cgcgccacga gcgtacccag gccagcgcga agatcgccgc
      121 gttgaggatc gcgaaatagc cgaacagcag cgccggctcg ccggtgacgc tggtcaggat
      181 cggcgcgaga aagccgccgg cgacggccag tgcggccagc ggctgcgagt cggcgcggat
      241 cgcaagcgcg acagtcgagg cggcgaccag tgcgagcagc aacaggcccg gcaccggcgg
      301 caggacgtga tacaggcgaa aggcggcgaa cgtcgtcaag tacaggatgc ctgcgcccgc
      361 gccctgcagc gaaagcccgt accccggtcg agcgcttgct agcaggatgc cgatggcgat
      421 cagcccgacg ccgataatgg ccacgccgac cagtcgccat tcgatcggga cagtgacgat
      481 ctcggcgaga tagcgcagca ggaacgccac gccgaaaaag agaatcacga cgccgatcct
      541 cgtcatcgcg ttgccgccgg tgagccatgc ccacaccgga tctggatcgg aactaggcgc
      601 cgcagccggt gcggacatcg cgacggacgc ggcagccggc ggcgcggaca tggtgagcgg
      661 ggcgacggcc ggcgcggcag aggcggtggc tagcgccgcc tctgccgcgg cttggacgaa
      721 tctgccgccg gtgccgcctt tgcgcaccgg cagcatggca ccggtcgcga cgccggctgc
      781 gtcgggtgac ggcgccagac taacggcttc gccggcctgg gccgccgccg tccggctttc
      841 gctcgagcgc gcggcagatt tgcgcaggta catgccgatc agcgcaccga tcaatgcacc
      901 gagcagcgcg ctcttgaaac cccagctgct gccgatgatg ccgccgatca acaagccgag
      961 ccaggtcatg ttgcccttcc ccgagccatc cggctagcgc cagtcgccgc gcaaggcgag
     1021 cacgttctgc tgcaggtatt ccggcgtcgc gcgctccggc ggcaccggcg gaccggcaac
     1081 cagtgcgatg cgcgagaaca ggccgcgaat cttgcgcatc gcgcggccgc gatctgaacg
     1141 gctgaagaaa ctgccccaca gtccgcgcag cgccatcggc acgaccggcg caggcgcctg
     1201 ttcgaggatg cgctgcaatc ctggacgaaa cggattgaga tcgccggtct cggtgagctt
     1261 cccctcggga aagatgccga cgatttcgcc agcccgaagc gccgcggccg cttccgcgaa
     1321 tgcgcgctcc ttcatcgccg cgtcctccct ggccggtgcg atcggaatcg tacgcatcgt
     1381 gcggaagatg aaattcaaca gaggaatgcg gaagatgcgg tgatccatca cgaaccggat
     1441 cggacggcgc acgcaggcgg caatgacgat tgcgtcgacg aagctgacgt gattgcagac
     1501 gatgacgcat ggcccttcgg gcggaatgcg ctcgaggcct tccttgtcga cgcggtagaa
     1561 cgtatggacc aggagccatg cgaggaaccg catcaggaat tcgggcacca gcaggtagat
     1621 gtagagcgca atggccgcgt tcatcagacc ggtgaccagc agcagctgcg gaatcgacag
     1681 acccgccttc agaagcgcca gggcgatgcc cgccgacgcg accatgaaga tcgcattgag
     1741 gatattgttg gccgcgatga tgcgcgagcg gaacgcgggc tccgagcgct cctggatcag
     1801 cgcgtacagc gggacgatat aaaagccgcc gaacataccg atcaggatca ggtctgcgat
     1861 gactcgccag tgcgcgccga cagcgaagaa tgccccgatg ccggcaagcc cctgcggcgc
     1921 caggttgcgg ctcgccagat acaaatcgat cgcgaacacc gacaggccga tcgagccgaa
     1981 cgggacgagg ccgatctcga ctttgtgccc cgacagccgc tcgcacagca gcgagccgac
     2041 cccgatgccg atcgagaaaa tcgcgagcag caaggtcgcg acgtgctcgt cgccgccgag
     2101 cacgtccttg gtgaagttgg cgaactgggt caggtaggtc gcgccataga accagaacca
     2161 cgagatgccc agcatcgcca gccatacgat gcggttgccg tgcgcgaagc gcaggttgcg
     2221 ccaggtctcg gtgaacgggt tccagttgat cctcaattcg ggcgcgactg ccggcgtctc
     2281 gggaatgcgg cgcgaaacca ggtagccggc gatcgc
//
LOCUS       DATANS010000026         1651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_715072, whole genome shotgun sequence.
ACCESSION   DATANS010000026 DATANS010000000
VERSION     DATANS010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715072"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VHT22_00385"
     CDS             complement(<1..643)
                     /locus_tag="VHT22_00385"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX3136032.1"
                     /translation="MSSSTHADASKSPLPTGTVTFLFTDIEGSTRLWESQQAAMQAAL
                     PRHDALVRQCITSHGGHVFKTGGDAFCAAFHTAPDALAAALESQRAIHAERWPEAAKL
                     RVRMAIHTGAAEFRDGDYFGAPLNRAARLLAAGHGGQTLLSESTHDLCRDHLPPLVTA
                     KALGEHGLKDLARREAVFQLCHPDLPQSFPPLKTSLAPLDRETPSIAVLPFINM"
     gene            757..1395
                     /locus_tag="VHT22_00390"
     CDS             757..1395
                     /locus_tag="VHT22_00390"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEX3136033.1"
                     /translation="MDRRQRIASIVAGVLLSTGALALDVNVVGLFPNKAVVQIDGGAL
                     QTLSVGQKTREGIVLLSVEPDGATFDIQGQRMALGLGHARMQPTTAVSAMATIDERGH
                     FVADGQINGVPIRFTIDTGATFISLPAGEARRLGLDYLKGRKAIMETANGVAAAYRVK
                     LDTVRVGDLTVNNVDAVVMEGDSLPVALLGMSFLNRMDIRREGQIMTLTKRY"
     gene            complement(1414..>1651)
                     /locus_tag="VHT22_00395"
     CDS             complement(1414..>1651)
                     /locus_tag="VHT22_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX3136034.1"
                     /translation="DAGVKVHVDAARKLLHSKFLVIDADRTIIGSHNWSAGSYFGFDD
                     LSVDVTSPAFARVTRQRFEDLWRRGTAASRDRQI"
BASE COUNT          289 a          532 c          549 g          281 t
ORIGIN      
        1 acatgttgat gaacggcagc accgcaatcg acggtgtctc gcggtcgagc ggcgcgagcg
       61 acgtcttaag cggcgggaaa ctctgcggca agtccggatg gcagagctgg aacaccgctt
      121 cgcgccgcgc gagatccttg agcccgtgct cgcccagcgc cttggcggtg accagcggcg
      181 gcagatgatc gcggcacagg tcgtgcgtcg actcggacag cagagtctgt ccgccatgac
      241 cggccgccag caggcgggcg gcgcgattga gcggggcgcc gaaataatcg ccgtcacgga
      301 attcggccgc gcccgtatgg atcgccatgc gcacgcggag cttcgccgct tccggccagc
      361 gctcggcgtg gatggcgcgc tgcgactcca gtgccgcggc cagtgcatcg ggcgccgtgt
      421 ggaacgccgc gcaaaaggca tcgccaccgg tcttgaacac atgaccgcca tgcgatgtga
      481 tgcactggcg gacgagtgcg tcgtgtcgcg gcagggcggc ctgcatcgcc gcctgctgcg
      541 actcccacag gcgcgtgctg ccttcgatgt cggtgaacag gaacgtcacc gtgccggttg
      601 gcagcggcga cttcgaagcg tcggcgtggg tgcttgagga cacggtcgtg tgcggatggg
      661 cgcggccagt cgttcgctgg cacgccgttt gcatggataa gactatcatg cgggtccgtc
      721 ccgaaaaaga ccactgattc gatcgccccc gttaccatgg atcgtcgcca acgcattgct
      781 tcgatagtcg ccggagtgct gctctcgacc ggcgcgcttg cgctggacgt caacgtcgtc
      841 ggcctgttcc cgaacaaggc cgtcgtgcag atcgacggcg gcgcgctgca gacgctgtcg
      901 gtcgggcaga agacccgcga gggcatcgtt ctgctgtcag tcgagcccga cggcgcgacg
      961 ttcgatatcc agggacagcg gatggcgctc ggactcggcc atgcgcggat gcagccgacc
     1021 actgccgtat ccgcgatggc caccatcgac gagcgcgggc attttgtcgc cgacggccag
     1081 atcaacggcg ttccgatccg attcacgatc gataccggcg cgacgttcat ctcgcttccc
     1141 gccggcgaag ctcgccgcct cgggctcgac tatctcaagg gtcgcaaggc gatcatggag
     1201 actgccaatg gtgtagcagc ggcctatcgc gtcaagctcg acaccgtgcg cgtcggcgat
     1261 ctgacggtga acaacgtcga tgcggtcgtc atggagggcg acagcctgcc ggtcgccctg
     1321 ctcggcatga gcttcctgaa tcgcatggac atccggcgcg aaggtcagat catgacgctg
     1381 accaagcgct attagttcgc cggccacgtc atatcagatc tgacgatccc tcgatgcggc
     1441 cgtgccgcgg cgccacaaat cctcgaagcg ctgccgcgtc acgcgcgcaa aagcgggcga
     1501 agtgacgtcg acgctcagat cgtcgaaacc gaagtaggag cctgccgacc agttgtggct
     1561 gccgatgatc gtccggtctg cgtcgatgac caggaacttc gaatgcagca acttgcgcgc
     1621 cgcgtcgacg tgaactttca cccccgcatc g
//
LOCUS       DATANS010000027         2752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_169181, whole genome shotgun sequence.
ACCESSION   DATANS010000027 DATANS010000000
VERSION     DATANS010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169181"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VHT22_00400"
     CDS             complement(<1..693)
                     /locus_tag="VHT22_00400"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX3136035.1"
                     /translation="MRFAHTFKTKAPAAPRLPRLRAPLVFGGLLLLFVGLLGRSLYLQ
                     RIDNGFLQEQGSSRYSRDIEVPAHRGRIVDRFGDPLAISTPVKAVWAWPDQVDATPDQ
                     LKALAAALDLPTTALKQKLAQGGDFVYLKKPVAPQAAAHIAALKIDGIHDENEYWRYY
                     PGGEVMSQIVGFTGDRDVGQEGIELAQQSWLGGHPGSRRVIINRRGEIVEDVASIRAP
                     QEGRDLALSIDSR"
     gene            complement(690..959)
                     /gene="ftsL"
                     /locus_tag="VHT22_00405"
     CDS             complement(690..959)
                     /gene="ftsL"
                     /locus_tag="VHT22_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEX3136036.1"
                     /translation="MARLNLALLALLVVCALSLVTSRYQARKLFVDLEREQAQSRAYD
                     VEYGQLQIEQSTWAMPARVEKIAREQLRMQLPAPARLRIVEAGPR"
     gene            complement(959..1921)
                     /gene="rsmH"
                     /locus_tag="VHT22_00410"
     CDS             complement(959..1921)
                     /gene="rsmH"
                     /locus_tag="VHT22_00410"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX3136037.1"
                     /translation="MPDGNHAPALLEEAVAALQIKPDGAYVDATFGRGGHASRILSLL
                     GPRGRLVALDRDPAAEAVARGWSDSRFVFRRAWFSELGSALDACGIAQADGVLLDLGI
                     SSPQIDDPERGFSLRADGPLDMRMDPTRGISAAEWIAHASERELRGVIADYGEERFAQ
                     QIARAIVAARTERPIVRTQQLAALVAKALGARARGDWSQDPATRTFQAVRIFINQELQ
                     ELSLALERVPARLAVGGRFAVISFHSLEDRLVKQAFRRGSQPYGGDARLARLAIASAS
                     LPKPPLKTVGRAIRPNDAELAVNPRARSATLRVAERTEGQLGPD"
     gene            complement(1921..2367)
                     /gene="mraZ"
                     /locus_tag="VHT22_00415"
     CDS             complement(1921..2367)
                     /gene="mraZ"
                     /locus_tag="VHT22_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX3136038.1"
                     /translation="MFRGVTQLALDNKGRLAIPAKHRETLAARDDGHLVLTADPGHCL
                     LLYPLSEWEPIQQRLMALSSFNDKLRSLQRLLVGHADDVVLDAAGRILVPPALRQYAA
                     LDRRVILVGQGNKFELWDEARWLERTSSAVVFPSGDLPPELDGFSL"
BASE COUNT          476 a          915 c          861 g          500 t
ORIGIN      
        1 cctgctgtcg atcgagagtg cgagatcgcg gccttcctgc ggcgcgcgta tgctggccac
       61 atcctcgacg atctcgcccc ggcgattgat gatcacgcgc ctgctgccgg ggtgcccgcc
      121 cagccagctt tgttgcgcca gctcgatgcc ttcctgaccg acgtcgcggt cgccggtgaa
      181 ccccacgatc tggctcatca cttcgccgcc cgggtaatag cgccagtatt cgttttcgtc
      241 gtgaataccg tcgatcttca gtgcggcgat gtgcgcagcc gcctgcgggg caaccggctt
      301 tttcaggtag acgaagtcgc cgccctgcgc gagcttctgt ttgagcgccg tcgtcggcag
      361 gtccagcgcc gcagccagcg ccttcagctg gtccggcgtt gcatcgacct ggtcgggcca
      421 ggcccatacc gccttgaccg gcgtgctgat cgcgagcgga tcgccgaagc gatcgacgat
      481 gcggccgcga tgcgccggca cctcgatgtc gcggctgtag cgcgagctgc cctgctcctg
      541 caggaacccg ttgtcgattc tctgcagata gagcgagcga cccagcagtc cgacaaacag
      601 cagaagcagg ccaccgaaga ccagcggcgc ccgcagccgc ggcagccggg gcgcagccgg
      661 cgctttggtc ttgaacgtgt gcgcgaatct catcgcggcc ccgcttcgac gatgcgaagg
      721 cgcgccggcg ccggcaattg catgcgcaac tgctcgcgcg cgatcttttc gacgcgcgct
      781 ggcatcgccc aggtcgattg ctcgatctgc agctgtccgt actcgacgtc gtaggcgcgg
      841 ctttgcgcct gctcgcgctc gagatcgacg aatagcttgc gcgcctggta gcgggaagtg
      901 accagcgaca acgcgcagac gaccagcagc gcgagcagtg cgagattgag acgcgccatc
      961 agtcaggccc tagctgccct tcggtgcgct cggctacgcg caaggtcgcg cttcgcgcgc
     1021 gcgggttcac cgcgagctcg gcatcattcg gccgtatcgc gcggccgacg gtcttcagcg
     1081 gcggctttgg caatgacgcg ctggcgatcg ccaggcgggc gagccgggcg tcaccgccgt
     1141 acggctggct gccgcgtcgg aaagcctgct tcaccagccg atcctcgagc gagtgaaagc
     1201 tgatcacggc gaaccggccg ccgaccgcca gccgcgccgg gacgcgttcg agcgctagcg
     1261 acaattcctg aagctcctga ttgatgaaaa tccgtacagc ctggaaagtc cgcgtggccg
     1321 gatcctgact ccaatcaccc cgcgccctcg cgccgagggc ttttgccacg agtgcggcca
     1381 gttgctgcgt ccggacgatc ggccgctccg tgcgagccgc aacaatcgct cttgcaatct
     1441 gttgagcaaa ccgttcttca ccatagtccg cgatcactcc cctgagttca cgttccgatg
     1501 catgcgcgat ccactccgcc gcgctgattc ctcgcgtcgg atccatgcgc atgtcgagcg
     1561 gtccatccgc gcgcagagag aatccacgct cggggtcgtc gatctgaggt gaagagatgc
     1621 cgaggtcaag cagcacgccg tcggcttgcg cgatgccgca ggcatcgagt gcggagccaa
     1681 gctccgaaaa ccatgcgcgc cgaaaaacaa agcgcgagtc gctccatccg cgtgcgaccg
     1741 cctcggccgc ggggtcgcga tcgagcgcga ccagtctgcc gcgcggaccc agcagcgaga
     1801 gaatgcggct ggcgtgtccg ccgcgcccga atgtcgcgtc gacataagcg ccatccggtt
     1861 tgatttgcag cgcggccacg gcttcctcga gcagtgccgg cgcgtggttc ccatccggca
     1921 tcagagcgag aaaccatcga gctcgggcgg cagatcgccg gacgggaata ccactgcgga
     1981 actcgttcgc tcgagccatc gcgcttcgtc ccaaagctcg aacttgttgc cttggccgac
     2041 caggatcacg cgtctgtcga gtgctgcgta ttggcgcagc gccggcggca cgaggatgcg
     2101 gccggccgcg tcgagcacga cgtcgtcggc gtgtccgacg agcaatcgct gcaggctgcg
     2161 gagtttgtcg ttgaagctcg agagcgccat cagccgctgc tgaatcggct cccactcgga
     2221 caacggatac aggagcaggc agtggcccgg gtcggcggtg agcaccagat ggccgtcgtc
     2281 gcgcgcagcc agtgtttcgc ggtgcttggc cggaatcgcc agccggccct tgttgtccag
     2341 tgccagttgc gtgacgcctc gaaacacctg ctcctccctt gccttgcacc gcgccgccgt
     2401 gggtacccac tttcacccac tttttaccac ttggccacta taaggaaacg agcaggaccg
     2461 gtcaacttaa attcgttttc cgaacaagga cttagctcat ttgttgtata catggctaaa
     2521 ttacttaaaa aataagtact tatgctattt tttgaatgta ttgcaataat attacgccgt
     2581 aacatccgcc ggatcgatcg gtggaggggc caaaaccgcc tgtccggcgg cttcatgacg
     2641 ccggcatggc cgtccccttc cggatgccgg gatcacaaac ggttacaacc tcggtggtca
     2701 acgcgccgac gaggcgcccg ttgttccgtc cagacgggca cgcgtcggaa ag
//
LOCUS       DATANS010000028         1776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_589504, whole genome shotgun sequence.
ACCESSION   DATANS010000028 DATANS010000000
VERSION     DATANS010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589504"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1618
                     /locus_tag="VHT22_00420"
     CDS             <1..1618
                     /locus_tag="VHT22_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3136039.1"
                     /translation="WYAAGLLDRIGGLLLLEFALAVSSDILGRTVSLLDSLLSEQFSN
                     VTSLRLMEHAATLDLEDFEDSELQDRLERARRQASGRMTLIGQLFGQIQDLVTIVSFG
                     AGLLVYAPWLIALLAVALVPAFIGEAHFNAQSYSLNYARTAERREIDYVRQTAASAET
                     AKEVKIFGLNRFLIDRYRRLAGDFYLANRTLALRRAGWGGLLAAVGTIAYYVAYAYIV
                     WRTLHGDFTIGDLTFLSGSFRRLRTLLENMLIGFSQVAGQALYLDDLFSFFEITPEIV
                     SPPNPRPFPLPLREGIRFEGVGFRYPGAERWAVRDLNFTLHAGEVLALVGENGAGKTT
                     VVKLLARLYDPDEGRILLDGHDLREYDLFTLRSNIGVIFQDFVRYHLTAAENIAVGRI
                     EAREDRARIAAAARQSLADDLIERLPQGYDQIIGKRFRTGVDLSGGEWQKIAIARAYM
                     RDAQLLTLDEPTAALDARAEFEIFQRFKELSKGKTAVLISHRFSSVRMADRIVVLADG
                     KIEAVGTHDELLAGGGRYAELFELQAAGYR"
BASE COUNT          290 a          579 c          570 g          337 t
ORIGIN      
        1 atggtatgcc gccggcctgc tcgatcgcat cggcggcctg ctgcttctgg aattcgccct
       61 cgcggtttcg tcggacattc tgggacgcac ggtctcgctg ctcgattcgc ttctgtccga
      121 gcaattcagc aacgtcacca gcctgcgcct gatggaacac gccgcgacgc tcgacctgga
      181 agatttcgag gacagcgagt tgcaggatcg gctggagcgc gcgcgccggc aggcttcggg
      241 tcggatgacc ttgatcggtc agctgttcgg ccagatccag gacctggtga cgatcgtcag
      301 cttcggcgcg ggactgctcg tctacgcgcc gtggctgatc gcgctgctgg cagtcgcact
      361 ggtgccggcg ttcatcggcg aggcgcactt caacgcgcaa agctattcgc tcaactacgc
      421 gcgcaccgcc gagcggcgcg aaatcgatta cgtccgacag accgccgcga gcgccgagac
      481 cgcaaaggaa gtcaagatct tcggcctcaa tcgcttcctg atcgatcgtt atcgacgact
      541 cgccggcgac ttctacctcg cgaaccggac gctcgcgctg cgccgggccg gctggggcgg
      601 cctgctggct gcggtgggaa ccatcgccta ttacgtcgcc tacgcctaca ttgtctggcg
      661 cacgctgcat ggcgatttta cgatcggcga tctgacgttt ctttccggct cgttccgtcg
      721 tctgcgaacc ttgctcgaga acatgctgat cggattctcg caggtggcgg gccaggcgct
      781 ctatctcgac gatctgtttt cattcttcga gatcacgccg gaaatcgtgt cgccgccgaa
      841 tccccgaccc tttccgctgc cgctgcgcga aggtattcgc ttcgagggcg tcggcttccg
      901 ttatccggga gccgagcgct gggcggtgcg ggatttgaat ttcacgttgc atgccggtga
      961 agttctcgcg ctggtcgggg agaacggcgc tggcaagacg acggtcgtca agctgctcgc
     1021 gcgcctgtac gaccccgacg aggggcgcat tcttctcgac gggcatgacc tgcgggagta
     1081 cgacctcttt accctgcgct cgaacatcgg cgtcatcttc caggacttcg tccgctatca
     1141 tctgaccgcg gcggagaaca tcgccgtcgg acgcatcgag gcgcgcgaag atcgcgcccg
     1201 catcgcggcc gcggccaggc agagcctggc cgacgatctg atcgagcgcc tgccgcaagg
     1261 ctacgatcag atcatcggca agcgcttccg caccggcgtc gacctgtcgg gcggcgaatg
     1321 gcagaagatc gccatcgcgc gcgcttacat gcgcgatgcg cagctcttga ccctcgacga
     1381 gccgacggcc gcgctcgacg cgcgcgccga attcgagatc ttccagcgct tcaaggagct
     1441 ctcgaagggc aagaccgcag tgctgatctc gcatcgtttt tccagcgtcc gcatggccga
     1501 ccgcatcgtg gtgctagccg acggaaaaat cgaagcggtt ggcacgcacg acgagctgct
     1561 ggcgggcggc ggccgctatg cggagctgtt cgaattgcag gcggccggct accgctaaca
     1621 agcgatcagg ccgcgaatcg ggtgtcggcg gtcggcgcct cgcagcggcg tgtacgcgat
     1681 ctttcgacca gcagcgaatc gccgaattcg tggctgcgat aagcgtgcgc tgccagcgca
     1741 acgccggccc ggcgcagggc ctcgtcgtcc gcgaac
//
LOCUS       DATANS010000029         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_169273, whole genome shotgun sequence.
ACCESSION   DATANS010000029 DATANS010000000
VERSION     DATANS010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169273"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="VHT22_00425"
     CDS             complement(<1..1387)
                     /locus_tag="VHT22_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136040.1"
                     /translation="MGDLIGVAGSVALPWLAGGCCMLALQRRAEQRDMILAAGYGYLL
                     GALATSLAMRALDAIGVRWTMPLVAMQLVALAVVACSMARLRSLPPLRERWQVSRARI
                     ADLPPAQRLLFFACLALIAVRLFGLGLELAWRPLLPWDAWAQWATKSRVWYEYGKIAA
                     FVDPATWLESGSGMQFVDAHPDYPATVPLLQVWTALCLGHWDESLINAPWLAILAALG
                     LAFYAQLRRIGIGMAQAMLFTYLLLSMPFIDLHVAVAGYVDIFIAAAYGMAAMALWQW
                     VRLRGRTDAFLAVLCALACIVIKKEGVVWALTLLPPVLVAINRRVGLGVVSLLGVAML
                     VYLLFGPSELRVLGYTLRTEFINVSRPMFEHFFEMDNWHLLWYLTLAVIALRWRTVFS
                     AATAPMTVTMLGAFAFVFVVFFYSSAAFGVADETLVNRLPLHMVPALAFYLAFLWTQA
                     PTRTSGVQSAAP"
     gene            complement(1422..>2256)
                     /locus_tag="VHT22_00430"
     CDS             complement(1422..>2256)
                     /locus_tag="VHT22_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136041.1"
                     /translation="RPNRNSSRRLTWSGTHIASLELGPDDGWTGTITGIALVARGTLP
                     RPLVLESFSVPSVSIATVIGDVFHQWAAFIPIVAASVTLPFDNERSHYATLLATVAAG
                     GLFAAAFYWLLTRRALSRDPRILWAIVVAGWLLLDLRFQVNLWRQLAQTAATFAGKTG
                     EEKRLASDDHELYVLMKEVRAGLPAPPVRVHFIADDQALRTRGSYFLYPQNIYYSDGT
                     RARKPVPEELRSGDYVLLFLTNDLGYDSAAQALVWPDGRRKPAEQILSRPPGLVLVRV
                     R"
BASE COUNT          473 a          750 c          730 g          303 t
ORIGIN      
        1 gcggagccgc gctttggacg ccggatgtcc gcgtcggcgc ttgcgtccac aggaatgcca
       61 gatagaacgc cagcgccggc accatgtgca acggcaggcg attgaccagc gtctcgtcgg
      121 cgacgccgaa agccgcgctg ctatagaaga acacgacgaa aacgaaggcg aacgcaccga
      181 gcatcgtgac ggtcatcggc gccgtcgcgg cggaaaaaac cgtgcgccag cgcaacgcga
      241 tcaccgccag cgtcaggtac cagagcagat gccagttgtc catttcgaaa aagtgctcga
      301 acatcgggcg cgacacgttg atgaattcgg tgcgcagagt gtagccgagc acccggagct
      361 cgcttgggcc gaacagcaga taaaccagca tcgcaacgcc gagcaaggac accacgccca
      421 ggccgacgcg acggttgatt gcgaccagca ccggcggcaa cagcgtcagc gcccacacca
      481 cgccctcctt cttgatcacg atgcaggcca gcgcgcaaag cacggcgaga aacgcgtcgg
      541 tgcggccacg caggcgaacc cactgccata gcgccatcgc cgccatgccg taggcggcag
      601 cgatgaagat atcgacgtat ccggcaaccg cgacgtgcag gtcgatgaac ggcatcgaca
      661 gcagcaggta ggtgaacagc atcgcctgcg ccattccgat tccgatgcgc cgcagctgcg
      721 cgtagaaggc gagtcctagc gccgccagga ttgcaagcca cggcgcgttg atcagcgatt
      781 cgtcccagtg gcccaggcat agcgcggtcc acacctgcaa cagcggaacc gtcgccggat
      841 agtcgggatg cgcgtcgacg aattgcattc cgctacccga ttcgagccag gtcgcgggat
      901 cgacgaaggc ggcgatcttt ccgtattcgt accagacgcg cgacttggtc gcccactgcg
      961 cccaggcgtc ccaggggagc agcggacgcc acgccagctc gaggcccagg ccgaacaagc
     1021 gcacggcaat cagcgccaaa caggcgaaga acagcaggcg ctgcgccgga ggcaggtcag
     1081 cgatgcgtgc gcgcgacact tgccagcgct cgcgcaacgg cggcagcgac cgcaggcggg
     1141 ccatactgca ggcgacgacg gcgagcgcta ccagctgcat cgcgaccagc ggcatcgtcc
     1201 atcgcactcc gattgcatcg agcgcgcgca tcgcaagcga cgtcgcgagt gccccaagca
     1261 gataaccata cccggcggca aggatcatgt cgcgctgttc ggcgcgacgc tgcagcgcaa
     1321 gcatgcagca gccgccggcg agccacggca gcgcgacgct tccggcgacg ccgatcaggt
     1381 cgcccatgcg tactctgcga ttccgccggg ggccagacgc ttcatcgcac gcggaccaga
     1441 acgagccccg gaggccgcga caggatctgt tccgccggct tgcgccggcc gtcaggccag
     1501 accagcgctt gggcagcgct gtcataaccg agatcgttgg tcaggaaaag cagaacatag
     1561 tcgccgctgc gcaattcctc cggcaccggc ttgcgcgcgc gcgtgccgtc gctgtaatag
     1621 atgttctgcg gatacaggaa atacgatccg cgcgtacgca gcgcctgatc gtcggcgatg
     1681 aaatgaacgc gcaccggcgg cgccggcaac cccgcgcgca cctctttcat caacacgtac
     1741 aactcgtgat catcggaggc caggcgcttc tcttcgccgg tcttgccggc gaacgtcgcg
     1801 gcagtttgcg ccagctgccg ccagaggttt acctggaagc gcaggtccag aagcagccag
     1861 ccggcgacga cgatcgccca aaggatgcgc gggtcgcgtg acaacgcgcg gcgggtcagc
     1921 agccaataga acgccgcggc gaaaaggccg ccggcggcca cggtcgcgag cagcgtcgcg
     1981 tagtgactgc gctcgttgtc gaatggcagc gtgaccgagg cagcgacgat gggaatgaac
     2041 gcggcccatt gatgaaagac atcgccgata accgtggcga tcgacaccga cggcaccgaa
     2101 aacgattcaa gcaccagcgg tcgcggcagg gtgccgcggg ctaccagcgc gatcccggtg
     2161 atcgtgccgg tccatccgtc atcggggccc agctccaacg aggcaatgtg cgtgccgctc
     2221 caggtcagtc gcctggaaga gttccgattc ggtcgt
//
LOCUS       DATANS010000030         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_421431, whole genome shotgun sequence.
ACCESSION   DATANS010000030 DATANS010000000
VERSION     DATANS010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421431"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..>1539
                     /locus_tag="VHT22_00435"
     CDS             178..>1539
                     /locus_tag="VHT22_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX3136042.1"
                     /translation="MTRIFELGPFRLDADAALLTREGTLTPLGPRAIRVLGTLVAHAG
                     GYVSKAKLLDTAWPGVVVEEGNLAVQVAAIRRVLAQAPGAERWIETLPRRGYRFVGPV
                     RELADERPEASDSKLTNLPEALTSFIGREREMVEVKRLLAGKRLVTITGAGGIGKTRL
                     AMQAAAEVVDAYGDGVWLAEFASIRDPSLVATTIAQTLGVPERAGTPATELLCAHLKS
                     RQVLLILDNCEHLLDSCAHLVNTLLPGAKGTTILATSREPLRVAGEQTYALPPLSLPE
                     SGAKAEAIRESEAVQLFVERVQRQLPEFELAGRSAAVAGICTHLDGIPLALELAAART
                     RSLSVEQINERLGDRFRLLTSGSRTALPRQQTLRATLDWSYDLLTEDERVVLRRLSVF
                     PGSFNVDAASAVASDAGIDGFAVIDLLSRLVERSLVVADTSSGDTRYRMLETTRAYGL
                     EKLA"
BASE COUNT          250 a          483 c          525 g          281 t
ORIGIN      
        1 aagagaaata aaacgagtgc gacgatccgt cgattgaagt gaggacgttt actgatttcc
       61 atctgggcct ccttgcaatg cctggccacg atgcggatcg tcgatcgacg gtcccccgca
      121 agtcttgaaa aaggcgttaa aaatctataa aaccggtgat gtccgggagg cttccgcgtg
      181 accaggatct ttgaactcgg gccgttcagg ctcgatgccg acgccgcctt gctcacccgg
      241 gagggaaccc tcacgccact tggtccccgc gccatccggg tgctgggcac gctggtggcg
      301 cacgcgggcg ggtacgtgtc gaaagccaag ctgctcgaca ccgcgtggcc gggcgtcgtg
      361 gtcgaggagg gcaacctcgc cgttcaggtc gccgcgatac ggcgcgtgct ggcccaggcg
      421 cccggcgccg agcgctggat cgaaacgctt ccgcgccgcg gctatcgttt cgtcggcccc
      481 gtgcgcgagc tcgcggacga acgtccggaa gcatctgaca gcaagctgac aaatctgccc
      541 gaggctctga cctcattcat cgggcgcgag cgtgagatgg tcgaggtcaa gcggctcctt
      601 gcggggaagc ggctggtcac gatcaccggc gcgggcggta tcggcaagac ccggctggcg
      661 atgcaggccg ccgccgaggt cgtcgacgct tatggtgatg gcgtgtggct cgccgaattt
      721 gcctcgatcc gtgatccctc gctggtcgcg actacgatcg cacagacgct cggcgtgccc
      781 gaaagagctg gcacgccggc gactgagttg ctgtgcgcgc atctgaagtc gcggcaggtt
      841 ttgctgatcc tggacaactg cgagcatctg ctggatagct gcgcgcatct tgtgaatacc
      901 ttgctcccgg gtgcgaaggg gacgacgatc ctggccacca gccgtgagcc gctgcgcgtt
      961 gccggcgagc agacctatgc gttgccaccg ctgtcgttgc ccgagtccgg agcgaaggcc
     1021 gaagcgatac gggaatcgga agcggtgcag ctcttcgtcg agagagtcca gcggcagttg
     1081 cccgagttcg agctcgcggg ccggtcggcg gcggtcgccg ggatatgcac gcacctcgac
     1141 ggcattccgc tggcgctgga gcttgccgcg gcacgcacgc gctcgctgtc ggtggagcag
     1201 atcaacgagc gtctcggcga tcgcttccgg ctgctcacga gcggatcgcg caccgcgctt
     1261 ccgcgtcagc agacgctgcg cgccacgctc gactggagct acgatctgct gaccgaggac
     1321 gagcgcgtcg tgttgcggcg gctgtcggtt tttccgggca gcttcaacgt cgacgctgcc
     1381 tccgccgtcg cctccgatgc gggcatcgac gggttcgcag tcatcgatct cttgtcgcgg
     1441 ctggtcgagc gttcgctggt cgtcgccgat acgagttcgg gcgacacgcg ctatcggatg
     1501 ctggagacta ctcgcgccta cgggttggag aaactcgcc
//
LOCUS       DATANS010000031         3209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_463354, whole genome shotgun sequence.
ACCESSION   DATANS010000031 DATANS010000000
VERSION     DATANS010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463354"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..865
                     /locus_tag="VHT22_00440"
     CDS             80..865
                     /locus_tag="VHT22_00440"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX3136043.1"
                     /translation="MSPARKPDYDLLIDIGNTFLKWGLFRADAVGEARENRVESGHVL
                     LEEIAGLAAQFAKFPTPAQIVISNVAGTRVRSTTIRVLETWPDAPSPHWLIPQDEQCG
                     VVNRYRNPAQLGSDRWAALIGARQLVGAKPALVVVCGTATTIDHLTAAGEFNGGVILP
                     GVGLMLRALHQHTAALPDADGEYVENPLQTVDAITSGCQHAQAGAIERVYRMHEAKTP
                     GLVCLISGGAARALAPRLIIPFELHDNLVLEGLYCISRTLAAA"
     gene            978..2168
                     /locus_tag="VHT22_00445"
     CDS             978..2168
                     /locus_tag="VHT22_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HEX3136044.1"
                     /translation="MSEQREVVILSGVRTAIGDYGGSLKDLPPTELAAKVVKEALSRA
                     KVDPKDVGQCVFGNVIHTEARDMYLSRVACVNGGLPHDTGALTLNRLCGSGLQAIVSA
                     SQYILLGDTEAAVAGGAESMSRAAYANVTQRWGARMGDAKDIDMMVGALTDPFDTIHM
                     GVTAENVATRCGITREQQDTFAVESHKRAAAAIAAGHFKSQVLPIELKSKKGPTVFDT
                     DEHVRGESTAEGMAKLRAVFAKENGTVTAGNASGINDAAAAVVLMEKSAAAKKGLKPM
                     ARLVAYGHAGVDPKVMGLGPVSAVKRALAKAGLKLEQMDVIESNEAFAAQALGVSKEL
                     GMNAAKVNPNGGAIALGHPIGATGCILTIKAMYELQRTGGRYALITMCIGGGQGIAAV
                     IERV"
     gene            complement(2169..>3209)
                     /locus_tag="VHT22_00450"
     CDS             complement(2169..>3209)
                     /locus_tag="VHT22_00450"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3136045.1"
                     /translation="DLPRVASIMTQLLRALDFAHERGVIHRDIKPANLILTADGTLKV
                     ADFGIARIDTSNLTTLGMVMGTPSYMSPEQCQGQAVDRRSDLFSAAVVLYELLAGEKP
                     FAGATETIAYKICHESPRPPSALSRLNLSPTIDAVLAVALEKSPDARFQNARAFSRAL
                     REALNAPAQQNPDETAATQVNTAISAMRPSIAAPPVWEDTVLRTVERQLAHYVGPMAK
                     VMTRKAATQTADLGKLYGLLAENISDPATREKFSAGMRLAAAPDATSPGHIATGTNTR
                     SMLRQAALEQSFVDQTSNQLAVYLGPIAKVVASRAAQKATSRQEFVRLVAGHLGDQER
                     NTFLKEIGFEEA"
BASE COUNT          560 a         1028 c         1100 g          521 t
ORIGIN      
        1 tgtatcgtta ccgcaaactc acgcggccga tctttccgct cgacccgatc tgacgaccga
       61 acaggacaaa aagaccgcga tgagccctgc ccgcaagccc gattacgatc tgttgatcga
      121 catcggcaac acgtttctca aatggggcct gttccgtgcc gatgccgtcg gcgaagcgcg
      181 ggagaaccgg gtcgaatccg gccacgtgct gctggaggaa atcgccgggc tcgccgcgca
      241 gttcgccaaa ttccccacgc cggcgcagat cgtcatctcc aatgtcgccg gcacacgcgt
      301 gcgctccacg acgatccgag tcctggagac ctggcccgac gcgccgtcgc cgcactggct
      361 gattccgcag gatgagcagt gcggcgtcgt caaccgctac cgcaatccgg cgcagctcgg
      421 cagcgatcgc tgggccgcgc tgatcggcgc gcggcagctg gtcggcgcca agccggcgct
      481 ggtggtcgtc tgcgggaccg cgacgacgat cgatcatctg accgccgcgg gcgaattcaa
      541 cggcggggtc attttgccgg gcgtcgggct gatgttgcgc gcccttcacc agcacaccgc
      601 cgctctgccg gacgccgatg gcgaatatgt cgaaaatccg ctgcagacgg tcgacgcgat
      661 caccagcggt tgccagcacg cgcaggccgg ggcgatcgag cgggtctatc gcatgcacga
      721 ggcaaagacg ccggggctgg tgtgcctgat ctcgggcggt gcggcgcgcg cgctggcgcc
      781 gcgactcatc atcccgttcg agctgcatga caacctggtg ctcgaggggc tttactgcat
      841 cagccgaacg ctcgccgcgg cctgatcccc gaccaattat cgcggcttgg ctccggatgc
      901 ggagccggtg aacgcccgac agtgcaccgc ggtcgtattg gcaggataat cgacgtcctg
      961 tcaccggagc caaaatcatg agcgagcaac gagaagtcgt catcttgagc ggcgtgcgca
     1021 ccgcgatcgg cgattacggc ggaagcctga aggatctgcc gccgaccgaa ctggccgcca
     1081 aagtggtcaa ggaagcgctg agccgggcga aggtcgatcc caaggacgtc ggccagtgcg
     1141 tcttcggcaa cgtgatccac accgaggcga gggacatgta cctgtcccgc gtcgcctgcg
     1201 tcaacggcgg cttgccccac gacaccggtg cgctgacctt gaaccgtctt tgcggcagcg
     1261 gactgcaggc gatcgtcagc gcgtcgcaat acatcctgct gggcgatacc gaggcagcgg
     1321 tcgcgggcgg ggccgagagc atgagccgtg cggcttacgc caacgtcacg cagcggtggg
     1381 gagcgcgcat gggggacgcc aaggacatcg acatgatggt cggcgcgctg accgacccgt
     1441 tcgacacgat ccacatgggc gtcaccgcgg aaaacgtcgc gacccggtgc ggcatcacgc
     1501 gcgagcagca ggatactttt gccgtcgaga gccacaagcg agccgcggcg gcgatcgcgg
     1561 ccgggcattt caagagccag gtcctgccga tcgagctcaa aagcaaaaaa ggtccgacgg
     1621 tgttcgacac cgacgagcac gtgcgcggcg agtcgaccgc cgaaggcatg gccaagctcc
     1681 gcgccgtctt tgccaaggaa aacggcaccg tgacggcggg caacgcgtcc ggaatcaacg
     1741 acgcggcagc tgcggtggtg ctgatggaaa aatcggcggc ggcgaaaaaa ggcttgaaac
     1801 cgatggcgcg actggtggcc tacggccacg ccggggtcga cccgaaagtg atgggactcg
     1861 ggccggtcag cgccgtcaag cgggcgctgg cgaaggcggg attgaagctc gagcagatgg
     1921 atgtgatcga atcgaacgaa gcgttcgccg cgcaggcact gggcgtaagc aaggagctcg
     1981 gcatgaacgc cgccaaggtc aatccgaacg gcggcgcgat cgcgctcggg catccgatcg
     2041 gcgcgaccgg ttgcatcctg acgatcaagg cgatgtacga attgcagcgc accggcgggc
     2101 gctacgcgct gatcacgatg tgcatcggcg gcggccaggg catcgccgcc gtcatcgagc
     2161 gcgtctagtc aggcttcctc gaagccgatt tccttcagga aggtattgcg ctcctggtcg
     2221 cccagatgcc cggcgaccag gcgcacgaat tcctgccggc tggtggcctt ctgcgccgcg
     2281 cggctggcga cgactttggc gatcggtccc aggtagaccg cgagctggtt gctggtctgg
     2341 tcgacgaagc tctgctcgag cgccgcttgc ctcagcatcg atcgcgtgtt ggtgccggtg
     2401 gcgatgtggc ccggcgaggt cgcatccggc gccgccgcca ggcgcatacc ggcgctgaat
     2461 ttctcgcgcg tggccggatc gctgatgttt tcggcgagca agccgtaaag ctttcccagg
     2521 tcggccgttt gcgtggccgc cttgcgcgtc atcaccttgg ccatcggacc gacatagtgc
     2581 gcgagctgtc gctcgacggt gcgcaggacc gtgtcttccc agaccggcgg tgcggcgatc
     2641 gacggtcgca tcgcagagat cgccgtgttg acctgcgttg ccgccgtctc gtcggggttt
     2701 tgttgtgccg gcgcgttcag cgcttcacgc agcgcgcggc tgaacgcgcg cgcgttctga
     2761 aaacgcgcat ccgggctttt ttccagtgcc accgccagga cggcgtcgat cgtcggagac
     2821 aggttgagcc gcgacagcgc cgacggcggc cgcgggcttt cgtggcagat cttgtaggca
     2881 atcgtctcgg tcgcgcccgc gaacggtttc tcgccggcga gcagctcgta gagcacgacc
     2941 gcagcgctga acaggtcgga gcgacggtcg accgcctggc cctggcattg ctcgggcgac
     3001 atgtacgacg gcgttcccat caccatgccg agcgtggtga ggttcgacgt gtcgatgcgc
     3061 gcgatgccga aatcggcgac cttcaacgtg ccgtcggcgg tgaggatcag gttcgccggc
     3121 ttgatgtcgc ggtggatcac gccgcgctcg tgcgcgaagt cgagcgcccg caacagctgc
     3181 gtcatgatcg acgccacgcg cggcaggtc
//
LOCUS       DATANS010000032         1880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_757305, whole genome shotgun sequence.
ACCESSION   DATANS010000032 DATANS010000000
VERSION     DATANS010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757305"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VHT22_00455"
     CDS             complement(<1..405)
                     /locus_tag="VHT22_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3136046.1"
                     /translation="MSAVPESCDVAVIGGGPAGSLAATYLTGKGYSVALFERQKHPRY
                     QVGESLLPDVWKYCDAAGVSDAIAAEGFLRKAGGSVDWNGEMRRLSFGDFGYTRPALH
                     VERDRFDHILFERARASGAWIGEEIAVLGADLS"
     gene            complement(402..665)
                     /locus_tag="VHT22_00460"
     CDS             complement(402..665)
                     /locus_tag="VHT22_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX3136047.1"
                     /translation="MSYSDQQIFDTLARFFAQEATGREGAIAVAPDTDIIGEGLVDSL
                     AIFKLIAFVEETFGVTIAPDEVLLENFQTLRAVRNLIVKKLPA"
     gene            complement(779..>1880)
                     /locus_tag="VHT22_00465"
     CDS             complement(779..>1880)
                     /locus_tag="VHT22_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3136048.1"
                     /translation="GPRGHDPSRPAYILYTSGSTGQPKGVVLSHRAARSFVDWAAETT
                     ALGPTDVVSSHAPLHFDLSVFDVFASCRAGACVTLVPPGLNVFPRNLADWIAAQEITV
                     WYSVPSLLARLALSGGLERHDWSRLRQIIFAGEIFPVAHLRRLREQIPGAHYWNWYGP
                     TETNVCTAYAVDALPPDQTAPVPIGTACANSRLLVVNEAGAECGPGETGELYVSGPSL
                     MNGYQAMPEATAHALVARGDGALWYRTGDLVREDAAGLLHLLGRRDGMIKSRGYRIEL
                     GEIESVLHQHPAVAEAAVIALPDEESGHVIRAAAVARVGQALDAGKLRAFCAERLPAY
                     MIPLSIAVRGSLPRGSTGKLDRMRLREELSDG"
BASE COUNT          342 a          627 c          598 g          313 t
ORIGIN      
        1 cgaaagatcc gcgcccagaa ccgcaatctc ctcgccgatc caggcgccgg aggcacgcgc
       61 acgctcgaac agaatgtgat cgaagcggtc gcgctcgaca tgcagcgcgg gccgcgtgta
      121 cccgaagtcg ccgaacgaaa gacggcgcat ctcgccgttc cagtcgacgc tgcccccggc
      181 cttgcgcagg aaaccctcag cggcgatcgc gtcgcttacc ccggccgcat cgcagtattt
      241 ccagacgtcg ggcagcagac tctcccccac ctgataccgc ggatgctttt gccgctcgaa
      301 cagcgcaacg gagtaaccct tgccggtcag gtaggtcgcg gcgagactcc ccgccggccc
      361 tcccccgatc accgcgacgt cgcaggattc gggaaccgcg ctcatgcggg cagcttcttc
      421 acaatcagat tcctgaccgc tcgcaatgtc tggaaatttt cgagaaggac ctcgtcggga
      481 gctatcgtta ctccgaacgt ctcttccacg aatgcgatca gcttgaaaat agccaacgaa
      541 tcgaccaggc cttcgccgat gatgtcggta tcgggcgcga cggcgatcgc tccctcccgg
      601 cctgtcgcct cttgggcgaa gaaccgtgcg agcgtgtcga agatttgctg gtcgctatag
      661 ctcatgtcgc ttgaagatct ccgtgtcggg atggctggcg gcgaactggt accgccctcc
      721 aacgccaaat tcgaggtggc gtgaggcatg cggcagtccg cagtgcgcgt cgaccgtttc
      781 agccatcgga cagctcctca cgcagccgca ttctgtcgag tttgccagtg cttccgcgcg
      841 gaagcgaccc gcgcaccgcg atcgacagcg gaatcatata cgctgggaga cgttcggcgc
      901 aaaacgcgcg caacttgccc gcgtccaatg cttgcccgac tctggcgacc gcggctgctc
      961 gtatcacgtg gccgctctct tcgtcgggta gagcgatcac ggcagcctct gcgacggccg
     1021 gatgctggtg caatacactc tcgatttcac ccaactcgat gcggtagccg cggcttttta
     1081 tcatgccgtc ccggcggccg agaagatgaa gcagcccggc agcgtcctcg cgtacgaggt
     1141 ctccggtgcg ataccagagg gcgccgtcgc cgcgagcgac aagagcatga gcggtggcct
     1201 cgggcatggc ctggtagccg ttcatcaagc tcggaccgct gacgtagagc tcgcccgttt
     1261 cgcccggtcc gcattccgcg cccgcctcgt tcacgacgag cagcctgctg ttggcgcaag
     1321 ccgtgccgat cggcaccggc gccgtctggt cgggcgggag cgcatcgacc gcgtaggcgg
     1381 tgcaaacgtt ggtctcggtc ggtccgtacc aattccagta gtgcgcgccg ggaatttgct
     1441 cgcgcagccg gcgcaaatgc gcgacaggga agatctcgcc ggcgaaaatg atctggcgaa
     1501 ggcgcgacca gtcgtgacgc tcaagaccgc ccgagagcgc gagtcgcgcg agcagcgacg
     1561 gcaccgagta ccagacggtg atctcctgcg cggcgatcca gtccgcgagg ttgcggggaa
     1621 agacattgag acccggcggc accagcgtta cgcaggcgcc cgcccggcag ctcgcgaaca
     1681 cgtcgaagac cgacagatcg aaatgcagcg gcgcatggct cgatacgacg tcggtcggac
     1741 ccagcgctgt agtctcggcc gcccagtcga cgaacgagcg cgcggcgcga tggctgagca
     1801 cgacgccctt cggctgtccg gtcgagcccg acgtgtagag gatgtatgcc ggacgggaag
     1861 gatcgtgccc tcgcgggccg
//
LOCUS       DATANS010000033         2443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_295184, whole genome shotgun sequence.
ACCESSION   DATANS010000033 DATANS010000000
VERSION     DATANS010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295184"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VHT22_00470"
     CDS             complement(<1..303)
                     /locus_tag="VHT22_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3136049.1"
                     /translation="MGTPSVVSPAYSAKTFDPRTGFGRLLSQVKTELMEALDRELAPL
                     DITAAQYVILATLAEGPTDSASNLCKGVSYDPGAMTRMIDRLERKGLVRRIRCPFDR"
     gene            482..>2443
                     /locus_tag="VHT22_00475"
     CDS             482..>2443
                     /locus_tag="VHT22_00475"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX3136050.1"
                     /translation="MISPGHYLRPLLAPESVALIGASAKAESLGRTVYQNLLAGGFKG
                     ELFAVNPNHATILDRPAFPSLTAIGKPVDLAVVCAPPAAVPAILDEMRQRVRGALILS
                     GAPTAKSADFQRWRGEIAARARASRIRVVGPASFGVIRTSLGLNASYSAVAAAPGRLT
                     LISQSGAVCGALLDFARTAGMGFASVVALGGTADVDFGEVLEFALADVETDGIVLYIE
                     TLRDARSFVSALRAAARTKPVIVLKAGRHPARIAAGDIDADRVFETALKRAGTVRVHT
                     YAQLFASTRILASGRIPRSNRLAIVTNGRGPGLLAMDRAAETDVVLADLSAGTRVALA
                     ALLPPEADPGNPVDVRGEATPARFGAAVTAVLADPGVDAVLALHVPVPAAPPAATAEA
                     VAAAARTAAKPVLAAWLGVIDQPGARVALESGGIANFFTPELAVDAFASVAAYRRNQE
                     WLLEVPPPLPALSAPDLPAAERVRAQAIAANRSRLADTETAALLAAFGIPAAPLSVVS
                     TVATARAFARRAGYPVMLERDGDAKAVRTGLRDGRMLERAWADLVADKSGRGRGRRGF
                     IVRKQLEFALSREVRIAVRTDPLFGPVIGYGATSAQARELALMLPPLNRRLAVDLIAG
                     AQLAGASPDVQPLVALLLELSALLCALPWV"
BASE COUNT          355 a          831 c          826 g          431 t
ORIGIN      
        1 gcgatcaaag ggacagcgaa tgcgccgaac cagacctttg cgttcgagcc ggtcgatcat
       61 gcgcgtcatc gcacccgggt catacgacac gcctttgcac aagttggatg ctgaatcggt
      121 cggaccttcg gccagagtgg ccaggatcac gtattgcgcc gccgtgatgt ccagcggcgc
      181 gagctctcga tcgagagcct ccatcagctc cgtcttgacc tggctcagca ggcgaccgaa
      241 gccggtgcgg gggtcaaagg tcttcgcgct gtatgcgggc gagaccacag aaggtgtacc
      301 catgtgacgg atgatagttg cctagacaat agttgtcaag gcctttatga tcccccgagt
      361 cgatggggcg agcctcttga gccacttact tgatatttaa cggttatttc ctacgagccg
      421 ggacggaatg gacgacttta ccgcactgca gcatcccaga aatttgcagt agactggcac
      481 cgtgatctct cccggccatt atctgcggcc gctgctggcg cccgagtcgg tagccttgat
      541 cggcgcctcg gcgaaggccg aatcgctggg ccgcaccgtc tatcagaacc tgcttgccgg
      601 tggcttcaag ggcgagcttt tcgcggtcaa cccgaatcac gccacgattc tcgaccggcc
      661 ggcatttccg tcgctgaccg cgatcggcaa gccggtcgac ctggcggtcg tctgcgcgcc
      721 gccggccgcc gtgccggcga ttctcgacga aatgcgccag cgggtgcgcg gagccctcat
      781 tctttccggc gcgccgacgg ccaagtccgc tgactttcag cgctggcgcg gcgagatcgc
      841 cgcgcgcgcc cgcgcctcgc gcatccgagt cgtgggcccc gcgagcttcg gcgtcatccg
      901 cacctcgctt gggctcaatg ccagctacag cgcggtcgcg gcggcgccgg gccggctgac
      961 cctgatctcg caatcgggcg cggtctgcgg cgcgctgctc gatttcgcgc gcaccgcggg
     1021 catgggattc gcgtcggtgg tagcactcgg cggaaccgcc gacgtcgact tcggcgaagt
     1081 gctggagttc gcgctggccg atgtcgaaac cgacggcatc gttctgtata tcgaaacgct
     1141 tcgcgatgcg cggagttttg tgtcggcctt gcgtgccgcc gctcgcacca agccggtgat
     1201 cgtgctcaaa gcgggacggc atccggcacg gatcgccgct ggcgatatcg acgcggaccg
     1261 cgtgttcgaa accgcgctca agcgcgcagg cacggttcgc gtgcatacct acgcgcaatt
     1321 gttcgcttcg acgcgcatcc tggcgtccgg gcggattccc cgcagcaacc ggctggcgat
     1381 cgtcaccaac ggcaggggac cggggctcct ggcgatggat cgcgccgccg aaacggacgt
     1441 cgtgctcgcc gatctctcag cggggacccg cgtcgcattg gcggcgctgc tcccgccgga
     1501 agccgatccc ggcaatccgg tcgacgttcg cggtgaggcg acgccagcgc gttttggcgc
     1561 cgcggtgact gcagtgcttg ccgatccggg tgtcgatgcg gtgctggccc tgcatgtccc
     1621 ggtgccggcc gcgccgccgg ccgccacggc cgaagctgtc gccgccgccg cgcgcacggc
     1681 ggccaagccg gtgctcgcag catggctggg ggtcatcgac cagccgggcg cgcgcgtcgc
     1741 gctggagtcg ggcggcatcg cgaatttttt caccccggag ctcgcggtcg atgcgtttgc
     1801 gtccgtcgca gcgtaccggc gcaaccagga atggctgctg gaagttccac cgccgcttcc
     1861 tgcgctcagc gctcccgatc ttccggcggc cgagcgcgtg cgtgctcagg cgattgcggc
     1921 aaatcggtcg cggcttgccg acaccgaaac ggccgcgctg ctcgccgcct tcggcattcc
     1981 ggcagcccca ctgagcgtcg tatcgacggt cgcgacggcg cgcgcgttcg cgcgccgcgc
     2041 cggctacccg gtcatgctcg agcgcgacgg cgacgccaag gcagtacgca ccggcctgcg
     2101 cgacggaaga atgctcgaac gggcctgggc ggatctcgtt gccgacaagt ccggccgggg
     2161 gcgcggcagg cgtggattca tcgtgcgcaa gcagctcgaa ttcgcgctgt cgcgagaagt
     2221 gcgcatcgcc gtgcgcaccg atccgctgtt cgggccggtc atcggctacg gggcaacgtc
     2281 ggcgcaggcg cgcgagttgg cgctgatgct gcctccgctc aatcgccggc tggccgtcga
     2341 tctgatcgcc ggcgcgcaac tggccggcgc atcccccgac gttcagccgc tagtcgcgct
     2401 gctgctcgag ctgtcagcgt tgctctgcgc gctgccttgg gtc
//
LOCUS       DATANS010000034         2884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_589881, whole genome shotgun sequence.
ACCESSION   DATANS010000034 DATANS010000000
VERSION     DATANS010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589881"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VHT22_00480"
     CDS             complement(<1..452)
                     /locus_tag="VHT22_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX3136051.1"
                     /translation="MSAVATGEFQHWQLTRDGDGLEWLTLDRAGASTNTLSAAVMEEL
                     RAVLARLAADPPRGLVIRSGKANGFIAGADVDEFGELKSVDDATALVRRGWDIFQELA
                     ATPYPTLALVRGFCLGGGMELALACRYRVAVDEPATRLGLPEVMLGIVP"
     gene            complement(449..1741)
                     /locus_tag="VHT22_00485"
     CDS             complement(449..1741)
                     /locus_tag="VHT22_00485"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX3136052.1"
                     /translation="MTEPVYIVDGTRTPFLKARNRPGPFAASDLAVQAGRALLSRMPF
                     APEELDEVILGCASPSPDEVNIGRVVALRIGCGLKVPGWTVMRNCASGMQALDSALTN
                     IRAGRSSLVLAGGVDALSRAPLLYSEKMVLWFAAFSAARTFGQKAGAFLKLSPAAMLT
                     PVIGIMKGLTDPVVGLMMGQTAENLAFRFGITRREMDAFAAESHRRVLAAQKAGHFDR
                     EIVPLYDRNGKLYPADDGVRQDSTPENLAKLKPFFDRKYGNVTAGNSSQITDGAAWMV
                     LASEAAVARHKLPVLGTIVDSEWAGLDPAEMGLGPVHAATPLLQRHHLALGDVDAWEI
                     NEAFAAQVIACLEAWESDDYCRNALGLPGALGRIDPARLNVDGGAIALGHPVGASGAR
                     IVLHLLNVLERTKGRRGIAAICIGGGQGGAMLVERANA"
     gene            complement(1785..>2884)
                     /locus_tag="VHT22_00490"
     CDS             complement(1785..>2884)
                     /locus_tag="VHT22_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase domain-containing
                     protein"
                     /protein_id="HEX3136053.1"
                     /translation="VIDGMDVAGGKGVCMGPSNFLGSAYMQLPVSITVEGANILTRSL
                     IIFGQGAIRCHPFVLREISAANDADRGRASSAFDLALFGHIGFVLSNMARTFVMGLCG
                     SHCARVPSDVAPETRRYYQQLTRFSAALAFLADISMGMLGGALKRKEKLSARLGDVLS
                     MLYLCSATLKRYENEGRQAEDAPLMHWAIWDAMFRAQNAIEGVISNFPSRMISGLLRT
                     MVFPLGRPYVVPSDKLGHDVARLLIAPSATRDRLTAGMYLPADEGDALGAIELALAAT
                     LAAEPVEDRMREAFKAGRFSAASGADRGAAAVAAGVISADELTLLQRAAELGVRVIRV
                     DDFAQDLGASELKPAAASAPASATSRKIAAA"
BASE COUNT          480 a         1017 c          941 g          446 t
ORIGIN      
        1 ggcacgatac ccagcatcac ctccggcagc ccgagccgcg tcgccggctc gtcgacggcg
       61 acgcgataac ggcaggcgag tgccagctcc atcccgccgc cgaggcaaaa gccgcgcacc
      121 agcgccaggg tcggataagg cgtggccgcc agctcttgga aaatatccca gccgcgacgg
      181 acgagcgcgg tcgcgtcatc gacgctcttg agctcgccga actcgtcgac atcggcgccg
      241 gcgatgaatc cgttggcctt tcccgagcgg atcaccaagc ctcgcggcgg atcggcagcg
      301 agccgcgcca atacggcgcg aagctcctcc atgaccgccg cggacagcgt gttggtcgac
      361 gcgccggcgc ggtccagcgt cagccattcg agtccatcgc cgtcgcgcgt gagctgccag
      421 tgctggaact cgccggtggc cacggcgctc atgcgttcgc gcgctcgacc agcatcgcac
      481 cgccctgtcc gccgccgatg catatcgccg caatccctcg cctgcccttc gtccgctcga
      541 gcacgttcag caagtggagc acgatccggg cgcccgaggc accgaccggg tggccgagcg
      601 cgatcgcgcc tccgtcgacg ttcagtctcg ccggatcgat ccggccgagc gctcccggca
      661 agccgagcgc gttacggcag tagtcgtcgc tttcccacgc ttccaggcag gcgatcacct
      721 gcgcggcgaa cgcctcgttg atctcccacg cgtcgacatc gccgagcgcg agatggtgcc
      781 gctgcagcaa cggcgtagcg gcatggaccg gccccagacc catttccgcc gggtctagtc
      841 ctgcccattc gctgtcgacg atcgtgccca gcaccggaag cttgtgcctg gcgaccgccg
      901 cttccgacgc gagcaccatc caggccgcgc cgtcggtaat ctgcgagctg ttgcccgcgg
      961 tcacgttgcc gtacttgcga tcgaagaacg gcttgagctt cgccaggttt tccggcgtcg
     1021 agtcttggcg cacgccgtcg tccgccgggt agagcttgcc attgcggtcg tacagcggga
     1081 cgatctcgcg atcgaagtgt ccggcctttt gggcggcgag cactcggcgg tggctctccg
     1141 ctgcgaacgc atccatctcg cgacgcgtga tgccgaagcg gaaggcgaga ttttccgcgg
     1201 tctggcccat catcagcccg acgaccggat cggtcaggcc cttcatgatt ccgatgaccg
     1261 gagtcagcat cgccgccgga ctgagcttca ggaacgcgcc ggctttctgt ccgaacgtcc
     1321 gcgccgcgct gaacgcggca aaccacagca ccattttctc cgagtacaac agcggcgcgc
     1381 gcgacagcgc atcgacgcca ccggcgagca ccaggctcga tcggccggcg cgtatgttgg
     1441 tcaacgcgga atcgagcgcc tgcatgccgg atgcgcagtt gcgcatcact gtccagccgg
     1501 ggaccttgag gccgcagccg atgcgcagcg cgacgacgcg gccgatattg acttcatcgg
     1561 gcgacgggct cgcgcagccg agtatcactt cgtccagctc ttccggcgcg aagggcatgc
     1621 gcgacagcag ggctctcccg gcctggacgg ccagatcgga agcggcgaac ggcccgggac
     1681 ggttacgcgc cttcaggaac ggcgtgcgcg taccgtcgac gatgtagacc ggctcggtca
     1741 tcgctgcctc gtcgtccgat cgccgctttc gggcgcgagg cggttcatgc ggcggcgatc
     1801 ttgcgactcg ttgcgctggc gggcgcggac gcagccgctg gcttgagctc ggacgcgccc
     1861 aggtcctgcg caaagtcgtc gacgcgaatc acccggacgc cgagctcggc tgcccgctgc
     1921 agcagcgtga gctcgtcggc gctgatgacg ccggccgcga ccgcggcggc gccacggtcg
     1981 gcgcctgacg ccgcgctgaa gcgcccggcc ttgaacgcct cccgcatacg gtcctcgacc
     2041 ggctcggcag cgagtgtagc cgccagcgcg agctcgatcg cgccgagcgc gtcgccctcg
     2101 tcagccggca aatacatgcc ggcggtcagc cggtcgcgag tggccgaggg cgcgatcaga
     2161 agccgcgcca cgtcgtggcc gagcttgtcc gaaggcacga catacggacg gccgagcggg
     2221 aacaccatcg tccgcagcag cccagaaatc atccggctgg ggaaattcga gatcaccccc
     2281 tcgatggcgt tctgtgcgcg gaacatcgca tcccagatcg cccagtgcat cagcggcgcg
     2341 tcttcggcct gccggccttc gttctcgtac cgcttgagcg tggccgagca cagataaagc
     2401 atcgacagca cgtcgcccag ccgcgccgac aatttttcct tgcgcttcag cgcgccgccg
     2461 agcatgccca tcgagatgtc cgcgagaaag gcaagcgcgg ccgagaaccg cgtcagctgc
     2521 tgataatagc gtcgtgtttc gggcgcgacg tcggaaggca cgcgcgcgca atgcgagccg
     2581 cacagtccca taacgaacgt tcgcgccatg ttcgacagga cgaagccgat gtggccgaat
     2641 agagccagat cgaacgcgct cgatgcccga ccgcgatcgg cgtcgttggc ggcggaaatt
     2701 tcacgcagca cgaacgggtg gcagcggatc gcgccctgcc cgaagatgat cagacttcgc
     2761 gtcaggatgt tcgcgccctc gacggtgatc gagaccggca gctgcatgta cgcggagccg
     2821 aggaagttcg acgggcccat gcacacgccc ttgcccccgg cgacgtccat gccgtcgatg
     2881 acca
//
LOCUS       DATANS010000035         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_421619, whole genome shotgun sequence.
ACCESSION   DATANS010000035 DATANS010000000
VERSION     DATANS010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421619"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VHT22_00495"
     CDS             <1..393
                     /locus_tag="VHT22_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3136054.1"
                     /translation="YAGISTGRTVWIGMLVGGATAGIAGVGEVAGPIGQLLPSISPGY
                     GFAAIIVAFVGRLHPLGIALASLLMSLLYLGGEAAQIGLELPSAVTRLFQGMLLFFLL
                     ATDVFIGYRLRFRRNPGPAAAAAESLAR"
     gene            390..1313
                     /locus_tag="VHT22_00500"
     CDS             390..1313
                     /locus_tag="VHT22_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3136055.1"
                     /translation="MIDHAITLFALTLAAGTVLVFAALGELVTEKAGVLNLGVEGMML
                     VGAVGAFIVADDTGHNWLGVLAGTLAGGAVSLIFAVLTLSLMANQVAAGLALSLFGIG
                     LSAFVGLTHVGHVVDPIAPLAVPVLSGLPVVGRLLFGHSPFVYLSLVLFALVQWFLFG
                     TRAGLILRAIGESPQSAHAIGHKVIAVRYAAVAFGGACAGLSGAYMALAYAPMWTEGM
                     TAGRGWIALALVVFANWKPSRVLAGAYLFGAVTLVQFQAQAMGVAVPSQFLAMLPYVA
                     TIVVLALISRDATVIRLNAPASLGRPFHADT"
     gene            1405..>2346
                     /locus_tag="VHT22_00505"
     CDS             1405..>2346
                     /locus_tag="VHT22_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3136056.1"
                     /translation="MSVSSKTVLFIVLAVFGALATITASAETPLKVGFVYVSPVGDAG
                     WSHQHDLGRREMERALGDRVRTTVVESVAEGADAERVIRELAQSGYQLIFTTSFGYMN
                     PTIKVAKQFPKTYFEHATGYKTDKNVGIYNARFYEGRYLAGIVAGRMTKSNIAGYVAA
                     FPIPEVVMGINAFTRGMRSVNPNAVTKVVWVNTWYDPGREREAADTLISQGADVLTNH
                     TDSTAVVQDAEAKKVYAIAYHSDMSKYGPHAQLTAVTHQWGAYYTKQAQNAIDGKWKP
                     GSIWGGLKEGMIKMGPFNPVVPKDVQDLVNRTATEIAS"
BASE COUNT          384 a          749 c          772 g          441 t
ORIGIN      
        1 tacgccggca tctccaccgg gcggaccgtg tggatcggca tgctcgtcgg cggcgcaacc
       61 gcaggcatcg caggagtcgg cgaagtcgcg gggccgatcg ggcagctgct gccttcgata
      121 tctccgggtt acggtttcgc cgcgatcatc gtcgcattcg tcggtcggct gcatccgcta
      181 ggcatcgctc tggcgagcct gcttatgtcg ctgctttacc tcggcggcga agccgcgcag
      241 atcggactgg aactgccttc ggcggtgacg cggctgttcc agggaatgct ccttttcttc
      301 ctgctcgcca cggatgtgtt cataggttac cgcctgcgct tccggcgcaa tcccggcccc
      361 gcagcggctg cagcggagag ccttgcccga tgatcgatca tgcgatcacg ctctttgcgc
      421 tgacgctcgc cgcaggaacg gtgctggtat tcgcggcgtt gggtgagctg gtaaccgaga
      481 aagccggcgt gctgaacctg ggtgtcgaag gcatgatgct ggtcggtgcg gtcggcgcat
      541 tcatcgtcgc cgacgacacc gggcacaact ggctcggcgt cctcgccggc acgctggccg
      601 gcggcgcggt gtcgctgata ttcgccgtgc tgaccttgtc gctgatggcg aatcaggtcg
      661 ccgcggggct tgcgctttcc ctcttcggga tcggcttgtc ggcgttcgtc ggcttgaccc
      721 atgtcggaca cgtcgtcgat ccgatcgcgc cgctggcggt gccggtgctc tccgggctac
      781 ccgtcgtcgg ccggctgttg ttcggccaca gtccgttcgt ctatctgtcg ctggtccttt
      841 tcgcgctggt gcaatggttc ctgttcggca cgcgcgccgg cctcatcctg cgcgcgatag
      901 gcgagtcgcc gcaatcggcg catgcgatcg gccacaaagt catcgccgtg cgctatgccg
      961 ccgtcgcgtt cggcggtgcc tgcgcgggct tgagcggcgc ctacatggcg ctggcgtatg
     1021 cgccgatgtg gaccgagggc atgaccgccg gacgcggctg gatcgcgctg gcgctggtcg
     1081 tgttcgcgaa ttggaagcct tcgcgcgttc tggccggagc gtatctcttc ggggcggtga
     1141 ccctggtgca gttccaggcg caggcgatgg gtgtcgccgt gccctcgcaa ttcctggcga
     1201 tgctgcccta tgtggcgacg atcgtcgtgc tcgcgctgat ctcgcgcgac gcgacggtga
     1261 ttcgcctgaa tgcgccggca tcgctcggtc ggccgttcca cgccgatacc taaagcgcag
     1321 atgggcacgc agcatcgagt aaaatgcccg attccgccgc cggcacaacg cgccggccgc
     1381 gcgcaacccg cctcgggaga gaccatgtcc gtgtcgtcga agaccgtgtt attcatcgta
     1441 ctagctgtgt tcggggcact tgcaacgatc accgcatcgg ccgaaacgcc gctcaaagtc
     1501 ggctttgtat acgtcagccc ggtcggcgac gccggctgga gccatcagca cgatctcggc
     1561 cggcgcgaga tggaacgggc gctcggcgac cgggtgcgca cgacggtcgt cgaatcggtc
     1621 gccgagggcg ccgatgcgga acgcgtgatc cgcgagctcg cgcagagcgg ctatcaactg
     1681 attttcacga cgtctttcgg ttacatgaat ccgacaatca aggttgccaa gcagtttccg
     1741 aagacgtatt tcgagcacgc gaccggatac aagacggaca agaacgtcgg catttacaac
     1801 gcgcgctttt acgagggccg ctacctcgcc ggcatcgtcg cgggacggat gaccaagtcg
     1861 aacatcgccg gttatgtcgc ggcatttccg attcccgaag tcgtgatggg catcaacgca
     1921 ttcacccgcg gcatgcgcag cgtcaatccg aatgcggtga ccaaggtcgt ctgggtcaac
     1981 acctggtacg acccgggccg cgagcgcgaa gccgccgaca cgctgatctc acagggcgcc
     2041 gatgtgctga ccaaccacac cgactcgacc gccgtcgtgc aggacgccga ggcgaagaag
     2101 gtgtacgcga tcgcctacca ctccgacatg tccaaatacg gtccgcacgc gcagctgacc
     2161 gcagtcacgc accagtgggg cgcgtactac acgaagcaag cgcagaatgc gatcgatggc
     2221 aaatggaagc cggggagcat ctggggcggg ctcaaggaag gcatgatcaa gatgggaccg
     2281 ttcaatcccg tcgtccccaa ggacgtgcag gacctggtca accggaccgc gacggaaatc
     2341 gccagc
//
LOCUS       DATANS010000036         1734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_169525, whole genome shotgun sequence.
ACCESSION   DATANS010000036 DATANS010000000
VERSION     DATANS010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169525"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VHT22_00510"
     CDS             complement(<1..966)
                     /locus_tag="VHT22_00510"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX3136057.1"
                     /translation="MTAPTASSAQGPLAGYRVLELGSTVAGPFCGRLFADFGAEVIKV
                     EAADGDPVRTMGSRYRDKSLWAASIFRNKSLIGVDLRKPRGQELIKTLVAKCDFLIEN
                     FRPGSLEAWGLGYEALAKINPGLIMVRISGYGQTGPYRERPGYGVICEAVSGIRHITG
                     DPDRPPGRIATSTTDYITGLYAAFGAMVALQSRHQTGRGQVIDAALYEGAFSFMEPHV
                     PAFEKLGIVAMRAGSRLPGNAPNNLYPTADEQYIHITAASDAVFRRIAKAIGEPALAS
                     DARFATALARVEHEDAIDDIITRWTLTHTCAQAEEKLHAAEVPAAR"
     gene            complement(975..1646)
                     /locus_tag="VHT22_00515"
     CDS             complement(975..1646)
                     /locus_tag="VHT22_00515"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3136058.1"
                     /translation="MPKIRTGPVRGDGAGALLPALRERIARHALPPGAKLLENDLARE
                     FGVSRAKVRDAFGVLEQRGLIQRIPNRGAVVARLDLSQVFEIYAVREMLEGLCVRLAV
                     ERTRPSSWQDLVQVFGRPMETIVKRGEFEKYIANLDELRSRTLEAAGNGVLADMLDSI
                     NDKTREIQRRIIILPGRAAEGLEQHRAVLAAMRKGDAAGAEELRRANIRSSRAYLERY
                     QSFVL"
BASE COUNT          291 a          542 c          581 g          320 t
ORIGIN      
        1 gcgcgcggcg ggaacctcgg ccgcgtggag cttttcctcg gcttgcgcgc aggtatgggt
       61 gagcgtccag cgtgtgatga tgtcgtcgat cgcgtcttca tgctcgacgc gcgcgagcgc
      121 ggtcgcgaaa cgcgcatcgg acgccagcgc gggctcgccg atggccttcg cgatgcgccg
      181 gaatacggcg tcgcttgccg cggtgatgtg gatgtattgc tcgtccgcgg ttggatacag
      241 attgttcggc gcattgcccg gcagccgcga gcccgcacgc atcgcgacga tgccgagttt
      301 ctcgaacgcc ggcacgtgcg gctccatgaa gctgaacgcg ccttcgtaca gcgcggcgtc
      361 gatgacttgc ccgcggcccg tctggtgccg cgactggagc gcgaccatcg ccccgaacgc
      421 cgcatacaga ccggtgatgt agtcggtggt ggaggtcgct atgcggccgg gcggacgatc
      481 ggggtcgccc gtgatatggc ggatgccgct gaccgcctcg caaatgacgc cgtagccggg
      541 gcgctcacgg taagggcccg tctgcccgta gccgctgatg cggaccatga tgaggccggg
      601 atttattttt gcgagcgctt cgtagccgag tccccacgct tcgagcgaac ccgggcgaaa
      661 attctcgatc aggaaatcgc atttggcgac cagcgtttta atgagttcct ggccgcgcgg
      721 cttgcgcaag tccacgccga tcagcgattt gttgcgaaag atgctcgcgg cccatagcga
      781 tttgtcgcga tagcgcgatc ccattgtgcg cacagggtcg ccgtcggcag cttcgacctt
      841 gatgacttcc gcgccgaagt cggcgaacag ccggccgcag aaagggcccg cgacggtcga
      901 gcccagttcg agcacgcggt agccggcgag cggaccttgc gcggatgatg cagttggtgc
      961 ggtcatgcgg gttcctaaag aacgaagctt tgatagcgct cgagatatgc gcgcgagctg
     1021 cggatgttgg cgcggcgcaa ttcttcggcc ccggccgcgt cgcccttgcg catcgcggcg
     1081 agcaccgcgc gatgctgttc caggccttcg gccgcgcggc ccggcaggat aatgatgcgg
     1141 cgctggattt cgcgcgtttt atcgttgatg ctgtcgagca tgtcggccag cacgccgttg
     1201 ccggcggcct ccagcgtgcg gctgcgcaac tcgtcgaggt tggcgatgta cttctcaaac
     1261 tcgccgcgct taacgatggt ttccatcggc cgcccgaaaa cctgcacgag atcctgccac
     1321 gatgacggcc tggtgcgttc gacggccagc cgcacgcaca agccttcgag catctcccgc
     1381 accgcataga tttcgaacac ctgggagaga tcgagccgcg cgaccaccgc gccgcgattg
     1441 gggatgcgct ggatgagccc gcgctgctcg agcacgccga acgcatcgcg cactttcgcg
     1501 cgcgacacgc cgaactcgcg cgcaaggtca ttctcgagca gtttggcacc gggaggcagg
     1561 gcgtgacgtg cgatgcgctc gcgcaatgcg ggcagcagtg cgccggcgcc atcgccgcgc
     1621 actggacccg tccggatttt aggcatcggc catcctagaa tttgctgacg aaagtgtcaa
     1681 caatcgtgtt gacgatcgac gctagctaaa atggtgcagt gccatatgag ataa
//
LOCUS       DATANS010000037         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_715436, whole genome shotgun sequence.
ACCESSION   DATANS010000037 DATANS010000000
VERSION     DATANS010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715436"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VHT22_00520"
     CDS             complement(<1..938)
                     /locus_tag="VHT22_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX3136059.1"
                     /translation="MSIERFAFPTAIYFGAGARKLVAEHLTAQGLERPLIVTDRGIAP
                     LPLLAKFIADLHRLDVAVYSDIWGNPVKSQVADGVTAFRKHRADSIIGLGGGAALDVA
                     KAIALMAVHQGDVLHYAWDHPQVRPIEHELPYFVALPTTAGTGSEVGRSSVISDDATH
                     IKKIIFSPRLLARAVFADPELTLDLPPAITAATGMDALTHNVESYLSPAYHPLCDGIA
                     LEGARIAARSLRTAVREPHNLQARSDMLMSSMMGAIAFQKDLGAVHSCAHALSTVADL
                     HHGLANGIMIDHVLRFNADAADTKMSELARVCGAGRS"
     gene            complement(935..>1502)
                     /locus_tag="VHT22_00525"
     CDS             complement(935..>1502)
                     /locus_tag="VHT22_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3136060.1"
                     /translation="KYDPAAARALLKEAGYPDGFQVTLDCVNVAYRAAVCQAITGMLA
                     QIGIRTTFQPWPTATFFPKLTQATTSFFEFGWSPQTDPWPMLNAIVHSYGVDGPGTFN
                     GGRYSNPRLDQLIDSIRVEPDIARRRQLVGDALRLMRADLPLLPLYRRTLTWVMRPEV
                     SVAQWPSDILELRFVRISDAQEVGSTAR"
BASE COUNT          266 a          468 c          523 g          245 t
ORIGIN      
        1 gagcggccgg cgccgcagac ccgcgcgagc tcggacatct tcgtgtcggc cgcatccgcg
       61 ttgaagcgca ggacgtggtc gatcatgatg ccgttggcga gcccatggtg cagatcggcg
      121 acggtcgaca gcgcatgcgc gcaggaatga acggcgccca ggtccttctg aaacgcgatc
      181 gcacccatca tcgagctcat cagcatgtcc gagcgcgcct gcagattatg cggctcgcgc
      241 accgcggtgc gcagcgaccg ggccgcgatg cgagcccctt cgagcgcgat gccgtcgcac
      301 aaagggtggt atgccggaga caggtacgac tcgacgttgt gggtcagcgc gtccatgccg
      361 gtcgccgcgg tgatcgccgg cggcagatcg agcgtcagtt cggggtcggc gaataccgcc
      421 ctggcgagca gccgaggcga aaagatgatc ttcttgatgt gcgtcgcatc gtcagatatg
      481 acgctcgagc ggccgacttc ggatccggtg cctgcggtcg ttggcagcgc gacgaaataa
      541 ggcagctcgt gctcgatcgg ccggacttgc gggtggtccc atgcgtagtg gagcacgtcg
      601 ccctggtgga ccgccatcag cgcgatcgcc ttggcgacat cgagcgcagc gcctccgccc
      661 agtccgatga tcgaatcagc gcgatgcttg cggaaggcgg tgacgccatc agcgacctgg
      721 cttttgaccg gattgcccca gatgtcggaa tacacggcga cgtcgaggcg atgcaggtcg
      781 gcgatgaact tcgcaagcag cggcaacgga gcgatgccgc ggtcggtgac gatcagcggg
      841 cgctccagac cctgcgcggt caggtgctcg gcaacgagct tgcgagcacc cgcgccgaag
      901 tagatagccg tcgggaaggc aaatcgctcg atgctcatcg ggcggtcgag cccacttcct
      961 gtgcgtcgct gattcgcacg aagcgcagct cgaggatatc gcttggccat tgcgcgacgc
     1021 tgacctcggg gcgcatcacc caagtcagcg tgcggcgata aagcgggagg agcggcaagt
     1081 cggcacgcat cagccgcagc gcgtcgccga caagctggcg ccggcgcgcg atgtcgggtt
     1141 cgacgcggat cgaatcgatc agttggtcga gacggggatt cgaatacctc ccgccgttga
     1201 acgtgccggg gccgtcgacg ccgtagctgt gcacgatcgc attcagcatc ggccaggggt
     1261 cggtctgcgg cgaccagccg aactcgaaaa agctggtcgt cgcctgcgtc agcttgggaa
     1321 agaacgtcgc cgtcggccaa ggctggaacg tcgtccggat gccgatttgc gctagcatgc
     1381 cggtgatcgc ctggcacacg gccgcgcggt aggcgacgtt gacgcagtcg agcgtcacct
     1441 gaaatccgtc cgggtagccg gcttccttca gcagcgcccg ggccgcggca ggatcgtact
     1501 tc
//
LOCUS       DATANS010000038         3381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_505991, whole genome shotgun sequence.
ACCESSION   DATANS010000038 DATANS010000000
VERSION     DATANS010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505991"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VHT22_00530"
     CDS             <1..492
                     /locus_tag="VHT22_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HEX3136061.1"
                     /translation="RDGAIEGVALRLPLTYGPGVKGNFARLLDAVAAERRLPLGGIDN
                     RRTLLYVGNAVSAIESALATPALAGETLPVADAESLSTSELAFHLARELDVAPRLFHV
                     PAVAMRAGGMLLGRRASVARLLGNLEVDAERFRKLARWMPPFTVDQGIAATAAWWKLR
                     HSL"
     gene            542..1279
                     /gene="rph"
                     /locus_tag="VHT22_00535"
     CDS             542..1279
                     /gene="rph"
                     /locus_tag="VHT22_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011656222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX3136062.1"
                     /translation="MRSPVTIRTGGRRADELRPLSIVRHYSRHAEGSALIECGATRVL
                     CMASVEESVPQFLKGKGQGWLTAEYGMLPRATNTRTRREAAEGKQSGRTQEIQRLIGR
                     SLRAIADLSQLGERTIRIDCDVLQADGGTRCASITGACVALYDAVAWCKAQGLLTGSP
                     LKDFVAAVSVGIVAGVPMLDLDYAEDSSCDTDMNIVMTGAGGFVEIQGTAEGAPFSHA
                     EMATLVALGEGGVATLIATQKTSLGIG"
     gene            1311..1910
                     /gene="rdgB"
                     /locus_tag="VHT22_00540"
     CDS             1311..1910
                     /gene="rdgB"
                     /locus_tag="VHT22_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX3136063.1"
                     /translation="MSEFSRLVIASNNPGKLIEFKRLLAPLGIETIAQAELGIDEADE
                     PHASFVENALAKARHASRRAGLPALADDSGICVEALRGAPGVQSARYAGEPRSDRRNN
                     ARLIADLDGVADRRAHYYCMLALMRHGDDPEPVLVDGRWDGTIIDSPRGDGGFGYDPH
                     FLDPATGMTGAELPLARKNELSHRGKAMRSLLDRLRSVA"
     gene            1920..3161
                     /gene="hemW"
                     /locus_tag="VHT22_00545"
     CDS             1920..3161
                     /gene="hemW"
                     /locus_tag="VHT22_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX3136064.1"
                     /translation="MVISATGLSLQTPHFAALPPLSLYVHIPWCLKKCPYCDFNSHER
                     RGEIPEARYVDALLADLELALPSIWGRRVHTVFFGGGTPSLFAPESIDRILAGVRARV
                     ALAPDAEITLEANPGTFERARFADFKAAGINRLSVGVQSFDPRFLHALGRVHDADEAR
                     AAAAAAIDIFGNVNLDLMYSLPGQTLGDAQSDLDEALRFSPPHLSFYHLTIEPNTLFH
                     RYPPALPDDETAADIEDAIAETLGAAGYLHYETSAYARPGHECRHNVNYWRFGDYLGI
                     GAGAHSKLSFQQRVVRQVRHKQPQQYIEEAERGTPLAEDRTVERNEIGFEFMLNALRL
                     TEGVPVALFAERTGFPLTLVQRSLDEAERRGLIARDHQHIRPTPLGQRFLNDLQAIFL
                     PAATPAAEAARRPVKLVRVKP"
     gene            3158..>3381
                     /locus_tag="VHT22_00550"
     CDS             3158..>3381
                     /locus_tag="VHT22_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX3136065.1"
                     /translation="MTPEPYALTLAGAADALSSGALSCTELTQSLLDRIDATDASVQA
                     WAWLDREHTLAAARALDARRTSGAGSTLEAV"
BASE COUNT          535 a         1164 c         1130 g          552 t
ORIGIN      
        1 cgcgacggag ctatcgaagg cgtcgcattg cgcttgcctc tgacctacgg ccccggcgtc
       61 aagggcaact tcgcgcggct cctcgacgcc gtcgccgccg agcgccggct gccgctcggc
      121 gggatcgaca atcgccgcac cttgctctac gtcggcaacg ccgtcagcgc gatcgagtcg
      181 gcattggcga cgccggcgct cgccggcgaa acccttccgg tggcggacgc cgaaagcctg
      241 tcgacgagcg agcttgcctt tcatctcgcg cgcgagctcg acgtcgcgcc tcgtctgttc
      301 cacgtgcctg ccgttgcgat gcgcgccggc ggcatgcttc tgggcaggcg cgcgagtgtc
      361 gcgcgcctgc tcggcaacct ggaggtcgat gcggagcgct tccgcaagct cgcccgctgg
      421 atgccgccgt ttaccgtcga ccagggtatc gccgcgaccg cggcgtggtg gaagctccgg
      481 cacagcctct agcgtcgagc ttgcgcgcgt ggcgcggcta taatcgagtc cctttcaagt
      541 gatgaggtcg ccagtgacca tccgcaccgg aggccgccgc gccgacgagc ttcgcccctt
      601 gtcgatcgtg cggcactaca gccggcatgc cgaaggctcc gcgctgatcg aatgcggtgc
      661 gacccgcgtg ctttgcatgg cgtcggtgga ggagtcggtg ccgcagtttc tcaagggcaa
      721 aggccagggc tggctgacgg cggaatacgg catgctgccg cgcgcgacca acacgcgcac
      781 gcgacgcgag gccgccgaag gcaagcagtc gggacgcacg caggagatcc agcggctgat
      841 cggccgcagc ctccgcgcca tcgccgacct gtcgcagctc ggcgagcgca cgatccgcat
      901 cgattgcgac gtgctgcagg ccgacggagg cacacgctgc gcatcgatca ccggcgcctg
      961 cgtcgcgctt tacgacgccg tagcgtggtg caaggcgcag ggtctcttga ccggctcgcc
     1021 gcttaaggat tttgtcgccg cggtgtcggt cggcatcgtc gccggtgtgc cgatgctcga
     1081 tctcgactac gccgaggatt cgagctgcga caccgacatg aacatcgtga tgaccggcgc
     1141 cggcgggttt gtcgaaatcc agggcacggc ggagggcgcg ccgttttcgc acgcggaaat
     1201 ggcgacgctg gtcgcgctgg gcgagggtgg cgttgccacg ctgatcgcca cgcaaaagac
     1261 ctcgctgggc atcggctgac gcagcgcgca atgacgaccg acagttcgac gtgagcgaat
     1321 tctcgcgcct ggtcatcgca tcgaacaatc caggcaagct gatcgagttc aagcgcctgc
     1381 tggcgccgct gggcatcgag acgatcgcgc aggccgagct cgggatcgac gaagccgacg
     1441 agccgcatgc gagcttcgtc gaaaacgccc tggccaaggc gcggcacgcc agccgccggg
     1501 ccgggttgcc tgcgctcgcc gacgattccg gcatttgcgt cgaggcgctg cgcggcgcgc
     1561 ccggcgtgca atcggcgcgc tatgccggag agccgcgctc cgaccggcgc aacaatgcca
     1621 ggttgatcgc cgatctcgac ggcgttgccg atcggcgggc acactattac tgcatgctcg
     1681 ccctgatgcg gcacggcgac gatcccgagc ccgtgctggt cgacggccgc tgggacggca
     1741 cgatcatcga ctcgccgcgc ggcgacggcg gcttcggcta cgatccgcat tttctcgacc
     1801 ctgccaccgg catgaccggt gccgagctgc cgctggcgcg gaagaacgag ctgtcgcatc
     1861 gcggcaaggc gatgcggtcg ctgctcgatc gactgcgcag cgtggcctag ggctcgagga
     1921 tggtgatttc cgccaccggt ttgtcgctgc agacgccgca tttcgcagca ctcccgccgc
     1981 tctcgctgta cgtccacatc ccatggtgcc tgaagaagtg cccgtactgc gacttcaact
     2041 cgcacgagcg gcgcggggag attcccgaag cgcgctacgt cgatgccctg ctcgccgacc
     2101 tcgagctcgc gctgccttcg atatgggggc gccgcgtgca caccgtcttt ttcggcggcg
     2161 gcacgccaag cctcttcgct ccggaatcga tcgaccggat actggccggc gtgcgcgcgc
     2221 gcgtcgcgct ggcgccggat gccgaaatca cgctcgaggc caatccgggc acgttcgagc
     2281 gggcgcgctt tgccgacttc aaggcggcag gcatcaaccg gctgtcggtc ggcgtgcaaa
     2341 gcttcgaccc gagattcctg cacgcgctcg gacgcgtgca cgacgccgac gaagcgcgcg
     2401 ccgctgccgc tgcagcgatc gatattttcg gcaacgtcaa tctcgacctg atgtactcgc
     2461 tccccggaca gacgctgggc gacgcccaga gcgatctcga cgaggcgttg cgcttttcgc
     2521 caccgcacct gtcgttttat catttgacga tcgagccgaa cacgcttttc catcgctatc
     2581 cgccggcctt gccggacgac gagacggcgg cggacatcga ggatgcgata gcggagacac
     2641 tcggcgccgc cggctacctg cactacgaaa cgtcggccta cgccaggccg ggtcacgaat
     2701 gccggcacaa cgtcaattac tggcgcttcg gcgattatct gggcatcggc gccggcgccc
     2761 attcgaagct gtcgtttcaa cagcgcgtcg tgcgtcaggt ccggcacaag cagccgcagc
     2821 aatatatcga ggaagccgag cgcgggacgc cgctcgccga ggatcgaacc gtcgagcgca
     2881 atgagatcgg cttcgagttc atgctcaatg cgctgcggct gaccgaaggc gtgccggtcg
     2941 cgttgttcgc cgagcgcacc gggttcccgc tgacgctggt gcaaaggtca ttggacgaag
     3001 ccgagcgacg cgggcttata gcacgcgatc accagcacat tcggccgacg ccgctgggcc
     3061 agcgttttct caacgacctg caggcgattt tcttgccggc cgccacccct gccgctgaag
     3121 ccgcgcgacg cccggtgaag ctcgtgcgcg tcaagccgtg acgcccgagc cgtatgcgct
     3181 cacgctggcc ggagcggccg acgcgctctc cagcggtgcg ctttcctgca cggaactcac
     3241 gcagtcgctg ctcgatcgca tcgacgccac cgacgccagc gtccaggcat gggcatggct
     3301 cgatcgcgag cacacgctcg ccgcggcgcg cgcgctggac gcgcggcgca cctcgggtgc
     3361 cgggtcgacg ctcgaagccg t
//
LOCUS       DATANS010000039         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_799378, whole genome shotgun sequence.
ACCESSION   DATANS010000039 DATANS010000000
VERSION     DATANS010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799378"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1206
                     /gene="purD"
                     /locus_tag="VHT22_00555"
                     /pseudo
     CDS             1..1206
                     /gene="purD"
                     /locus_tag="VHT22_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478716.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
     gene            1319..2446
                     /locus_tag="VHT22_00560"
     CDS             1319..2446
                     /locus_tag="VHT22_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX3136066.1"
                     /translation="MANASPAKTLTVPASMKAWVLGNPDELKLVDKPVPQPGAAEVLV
                     RIDAIAVCATDLEIIHSGPPALIQGGAPFNKNFTPGHEYMGTVVKLGPGVDEFDIGDR
                     VTVEIHAGCGRCERCREGMYTACTNYGMNYGDRNKGHRANGFTTDGGFAQFAVNNINT
                     MVHVPDDMSDEEATLVVTAGTAMYGLDVMGGLIAGQTVVVMGPGPIGLMGVGVAKALG
                     AQPVILTGTRDNRLEIGRKLGADHVINVRKEDAVEAVKRITHGKGAHYVLECSGAPTA
                     VNEAARMLNRGGRICLAAFPHEPALVDVAYLVRNNIYLFGIRGEGKSATHRAAAFMAQ
                     KRFDAKLIHTHTFPLAEVPTAIKYARERIDDAIKVVVKVHQ"
BASE COUNT          473 a          794 c          814 g          370 t
ORIGIN      
        1 gtctcctagg tttacgtcgc ccccggcaac gcgggcaccg cgcgcgagga cggcgtcaag
       61 aatgtcgaca tcaccgacca tgacgcactc gtcgcctttg cgcagcagga gcagatcgcg
      121 ctgacggtgg tcggccccga agcgccgctg gccgcagggg tggtcgactc gtttcaggcg
      181 gcgggactta aaatctttgg ccccacgcag cgcgcggcgc aactcgaaag ctccaaagat
      241 tttgccaagg ctttcatgca gcggcatcgc ataccgactg ctgcgtacca gacgtttacc
      301 aacgcgcagg ccgcgcatga atacgtcgac gggcgcggcg ccccgattgt gatcaaggcc
      361 gacgggctcg ctgccggcaa aggcgtggtc gtcgccgcga gcctcaaaga agcgcacgac
      421 gcgatcgaca tgatgttgac cgacaacaag atgggggctg ccggcagccg cgtggtcatc
      481 gaggattttc tcgacggcga ggaggcgagc ttcatcgtga tggtggacgg ccggcacgcg
      541 ctggcgctcg cgacgagcca ggaccacaag cggctcaaag acggcgacgc cgggcccaat
      601 accggcggca tgggcgctta ttcgccggcg ccggtggtaa cgcccagcgt gcacgcgaaa
      661 gtcatgcgcg aagttatcaa gccggcgatc gccggcatgg agcaggatgg cgcgccgttc
      721 gtcgggttcc tgtatgcggg gctgatgatc gacaaggcgg gacaggtcca cgtggtcgaa
      781 ttcaactgcc ggctgggcga cccggaaacg cagccgatca tcctgcggct caaaagcgac
      841 ctggtgacgc tgttcgacca cgcgctcgcc ggcacgctcg atcaagccga agccgaatgg
      901 gatccgcgcg cggcgctggg tgtggtgctc gccgcgcatg gctatcccga cgcgccgcgc
      961 aaaggcgatg cgatcaccgg gttgccgccc gcgggtgacg attaccacgt gtttcattcc
     1021 ggcaccgcgc tcgacggcga ccaggtcgtt gcgaccggcg gccgcgtatt gtgcgtagcg
     1081 gcgctcggcc acaatgtgaa gacggcgcaa cgccgcgcgt atgaaatcgc cgggtccata
     1141 cgctttgacg gtatgcagat gcgccgcgac atcggccatc gcgcgacgca caatcagcgg
     1201 cgctgagccg cgcgccaggc atccgggaag cgcgtgctag aataattttc cgctgcgcga
     1261 ggcgcggtcc accgatgcaa ccgtgcgccg acaatgcgta acgcgagagg agaaattgat
     1321 ggcaaacgcc agcccggcca aaactcttac cgtccccgcc agcatgaagg cctgggtgct
     1381 cggcaacccc gatgaattga agctcgtcga taagcccgtg ccgcagcccg gcgctgccga
     1441 agtgctcgtg cgcatcgatg cgatcgcggt gtgcgcgacc gatctcgaaa tcatccacag
     1501 cgggccgccc gcgctgatcc agggcggcgc tccgttcaac aagaatttca cgcccggcca
     1561 cgaatacatg ggcaccgtcg tcaaactcgg ccccggtgtg gacgagttcg acatcggcga
     1621 tcgcgtgacg gtggaaatcc acgccggttg cgggcgctgc gagcgttgcc gcgagggcat
     1681 gtacaccgcg tgcacgaatt acggtatgaa ctacggcgac cgcaacaagg gccaccgcgc
     1741 caatggtttc accaccgacg gcgggttcgc gcagttcgcg gtcaacaaca tcaacaccat
     1801 ggtgcacgtg cccgacgaca tgagcgacga ggaggccacc ctcgtggtca ccgccggcac
     1861 ggcaatgtac gggctcgacg tgatgggcgg cctgatcgcc ggccagacgg tggtcgtcat
     1921 gggtccgggc ccgatcgggc tgatgggtgt cggcgtcgcg aaagcgctcg gcgcgcagcc
     1981 ggtgatcctg acgggcacgc gcgacaaccg gctggaaatc ggccggaaac tcggcgcgga
     2041 ccacgtgatc aacgtgcgca aggaagacgc ggtcgaggcc gtcaagcgca tcacgcatgg
     2101 caagggcgcg cactacgtgc tcgaatgctc gggcgcgccc actgcggtca acgaagccgc
     2161 gcgcatgctg aaccgcggcg gccgcatctg ccttgccgcg tttccgcacg agccggcgct
     2221 cgtcgacgtc gcgtatctcg tgcgcaacaa catttatctg ttcggcattc gcggcgaagg
     2281 caaaagcgcg acccatcgcg cggccgcgtt catggcgcag aagcgcttcg acgcgaagct
     2341 catccacacg catacgttcc cgctcgcgga agtgccgacc gcgatcaagt acgcgcgcga
     2401 gcgcatcgac gacgcgatca aagtagtcgt caaagtccac cagtaagcat g
//
LOCUS       DATANS010000040         2834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_127740, whole genome shotgun sequence.
ACCESSION   DATANS010000040 DATANS010000000
VERSION     DATANS010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127740"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1592
                     /locus_tag="VHT22_00565"
     CDS             <1..1592
                     /locus_tag="VHT22_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3136067.1"
                     /translation="ALEPFRRLQGQMTLIALLGVIISVFASIFIARGIARPVHDLAGI
                     ALRIAAGDYSTAPPVSRTDEIGDLANAFRNMQQDIASRESRIMDLAYRDGLTQLPNRA
                     LYNDRLDEALATAAALGAPVAVLLMDLDHFKDVNDSLGHPIGDLILCAVSSRLELVLK
                     RPTDTVARLGGDEFAILLPGDDAAKAERVAKALLHALDMPMTPEGHVVDVRASIGIAV
                     YPEHGSERSTLLRHADAAMYAAKRRNLGIALWDERYDLHSRERLSLMSGLRKAVDEDE
                     LVLFYQPKVALRGGSELHAEALVRWQHPTRGLVSPVEFIAFAEQTGYIRAITQWVMAH
                     AIAQCAAWRGEDLPMNVSINISARDLVDLELPDRVEALLHKHGCAAQWITLEITESAI
                     LDDPDHAIDNLRRLHSLGCRLAIDDYGTGYSSLAYLRRLPVHELKIDKTFILGMARDT
                     SDAVIVRSTIDLAHHMGLVVVAEGVEDEATVERLRGLSCDMVQGYLLSRPLAAADMVS
                     WMRSSVWTRAARDPGLLRRVV"
     gene            1735..>2834
                     /locus_tag="VHT22_00570"
     CDS             1735..>2834
                     /locus_tag="VHT22_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dienoyl-CoA reductase FMN-binding
                     domain-containing protein"
                     /protein_id="HEX3136068.1"
                     /translation="MAPYPHLLAPLPLGFTTLKNRVLMGSMHTGLEEAPNGFDKLAAF
                     YAARARGGVGLIVTGGIAPNFSGRIDPHHSQLSFAWQVAKHRMVTDAVHAAGGRVAMQ
                     ILHGGRYSYHPLAVAPSALKSPITPFRPRALTGWGVRRTIAAYVRCAALAQRAGYDGV
                     EIMGSEGYLINQFVAPQTNLRDDEWGGSFLNRIRFPVAVVRRTREAVGPDFIIIYRLS
                     MLDLVEGGSSWDEVVALAQAVEAAGATIINTGIGWHEARIPTIATMVPRGAFAWVTRR
                     LKGAVRVPLVTTNRINDPAVAEAILARGDADMVSMARPFLADADFVAKAAHGRADEIN
                     TCIGCNQACLDQIFERQTATCLVNPYACRETEL"
BASE COUNT          451 a          960 c          944 g          479 t
ORIGIN      
        1 cagccctcga gccgttccgc cgcctgcagg gtcagatgac gctgatcgcg ctgctgggtg
       61 tgatcatctc ggtattcgcc agcattttca tcgcccgcgg catcgcgcga ccggtgcacg
      121 atctcgcggg catcgccttg cgcattgccg ccggcgacta ttcgaccgcg ccgccggtat
      181 cgcgcaccga tgagatcggc gatctcgcca acgccttccg caacatgcag caggacatcg
      241 cgagccggga atcccgcatc atggacctgg cgtaccgtga tggcctgacg cagttgccga
      301 accgggcgct gtacaacgac cgcctcgacg aggcgctggc caccgcagcg gccctcggcg
      361 cgccggtcgc ggtgctgctg atggacctcg atcacttcaa ggacgtcaac gactcgctcg
      421 gccatccgat cggagacctg atcctgtgcg cggtttcgtc ccgactcgag ctcgtgctca
      481 agcggccgac cgataccgtc gcgcgcttgg gcggcgacga gttcgcgata ctgctgcccg
      541 gcgacgatgc ggccaaggcc gaacgcgtcg ccaaggccct gctgcatgcg ctggacatgc
      601 cgatgacgcc cgaaggtcac gtcgtcgacg tgcgcgcgag catcggcatc gccgtctatc
      661 ccgagcatgg cagcgaacgc tcgaccctgt tgcggcacgc cgatgccgca atgtacgcgg
      721 ccaagcgcag gaatctcggc atcgcgctgt gggacgagcg ttatgacctg cacagcaggg
      781 agcgactgtc gttgatgagc ggcttgcgca aggccgtcga cgaagacgag ctcgtgcttt
      841 tctaccagcc caaggtcgcg ctgcgcggcg gcagcgaact gcacgccgag gcgctggtgc
      901 gctggcagca tccgacgcgc gggctggtct cgccggtcga gttcatcgcc tttgccgagc
      961 agaccggcta catacgcgcg atcacgcagt gggtgatggc acacgcgatc gcgcaatgcg
     1021 ccgcctggcg cggcgaagac ctgccgatga acgtgtcgat caatatttcg gcgcgcgacc
     1081 tggtcgacct cgagctaccg gaccgcgtcg aggcgctgct gcacaagcac ggctgcgcgg
     1141 cgcagtggat cacgctggaa atcaccgaaa gcgcgatcct cgacgatccg gatcatgcga
     1201 tcgacaacct gcggcggctg cactcactgg gctgccggct ggcgatcgac gactacggca
     1261 ccggctactc gtcgctcgcg tacctgaggc ggctgccggt gcacgagctc aagatcgaca
     1321 agacgttcat cctgggcatg gcacgcgaca ccagcgacgc ggtgatcgtc cgctcaacga
     1381 tcgaccttgc gcaccacatg ggcctggtcg tcgttgccga aggtgtcgag gacgaagcga
     1441 cggtcgagcg gctgcgcggc ctgagctgtg acatggtcca gggctatctg ctcagtcgtc
     1501 cgctcgctgc ggcggacatg gtctcgtgga tgcgctcgtc ggtctggaca cgcgccgcgc
     1561 gcgacccggg gctgctgcgt cgtgtcgtct gaagccgagg ttgtgggcga cccgcccgca
     1621 gcgatgattt tcgcaagcag cggccagggc tgttgacgca tttagcgtga acaggcctta
     1681 atcgtttacg ctgtcggcct tgacggcgcg caccgtgcgt cgtgaccggc atccatggct
     1741 ccctacccgc atctgttggc tccgctcccg ctcgggttca ccacgctgaa gaaccgcgtg
     1801 ctgatgggct cgatgcatac cgggctcgaa gaggcgccga acggctttga caagcttgcc
     1861 gctttctacg ctgcgcgcgc ccggggcggc gtcgggctga tcgtcaccgg aggcatcgcg
     1921 ccgaatttca gcggacggat cgatccgcac cattcgcagc tttcgttcgc ctggcaggtc
     1981 gcgaagcacc gcatggtcac cgacgccgtg cacgctgccg gcggcagggt cgcgatgcag
     2041 atcctgcacg gcggccgcta ttcctatcac ccgctcgccg ttgcgccgtc ggcactgaag
     2101 tcgccgatca cgccgtttcg gccccgcgcg ctgacgggct ggggcgtgcg ccggacgatc
     2161 gccgcgtacg tccgctgcgc cgcgctcgcg cagcgcgccg gctacgacgg cgtcgagatc
     2221 atgggctcgg agggctatct gatcaatcag ttcgtcgcgc cgcaaacgaa tctgcgcgac
     2281 gacgagtggg gcggatcgtt tctcaaccgc atccgctttc cggtcgccgt cgtgcgccgc
     2341 acgcgcgaag cggtcggccc ggacttcatc atcatctatc gcctgtcgat gctggatctg
     2401 gtcgaaggtg gcagctcctg ggacgaggtt gtcgcgctgg cgcaggcggt cgaagccgcc
     2461 ggcgcgacga tcatcaacac cggcatcggc tggcacgagg cgcggatccc gacgatcgcg
     2521 acgatggtgc cgcgcggggc gttcgcgtgg gtgacgcgcc ggctcaaggg cgcggtgcgc
     2581 gtgccgctcg tcaccaccaa ccgcatcaac gacccggctg tggccgaggc gatcctcgcg
     2641 cgcggtgacg ccgacatggt ctcgatggcg cgtccattcc tcgccgacgc cgacttcgtc
     2701 gccaaggcgg cgcacggacg cgccgacgaa atcaacacgt gcatcggatg caaccaggcg
     2761 tgtctcgacc agatattcga gcggcagacg gcgacctgcc tggtcaatcc ttacgcgtgt
     2821 cgcgagaccg agct
//
LOCUS       DATANS010000041         3472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_589997, whole genome shotgun sequence.
ACCESSION   DATANS010000041 DATANS010000000
VERSION     DATANS010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589997"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VHT22_00575"
     CDS             <1..556
                     /locus_tag="VHT22_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX3136069.1"
                     /translation="LQRQILFGTADVGRPKVDAAAERIAAVNPEVRVERVARRVGPQD
                     LAPLAAEADIVLDCSDNFATRHAINRACVAAKKPLVSGAAIRFDGQIAVFDARDGSHP
                     CYHCLFGEGSDFEETRCAVMGVFAPLVGIVGATQAAEALKLIAPAGRSLAGRLLLVDA
                     LTMEWREVRVLKDPACPVCGTPAR"
     gene            595..1017
                     /locus_tag="VHT22_00580"
     CDS             595..1017
                     /locus_tag="VHT22_00580"
                     /inference="COORDINATES: protein motif:HMM:NF027482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4864 domain-containing protein"
                     /protein_id="HEX3136070.1"
                     /translation="MTRLAVVGIACWLGVAAAAQTAPTAAEWQAIQQVIAAQRAAIIA
                     GEAEKAFSYASPGIQQQFGDAASFLAMVDAAYAALESARYVEFLEGAVIEGLVVQPLR
                     LIDADNTVRVALYTMEKQANGEWRISSCRIAPSTVRAA"
     gene            complement(1042..1611)
                     /gene="slmA"
                     /locus_tag="VHT22_00585"
     CDS             complement(1042..1611)
                     /gene="slmA"
                     /locus_tag="VHT22_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HEX3136071.1"
                     /translation="MASKPGERRLQILQTLAAMLETPRGEKVTTAALASRLDVSEAAL
                     YRHFASKAQMFEGLIEFIETTLFSLVNRISSESTDGVAQATQMVAVLLGFAEKNPGMT
                     RVLIGDALVNEDERLQARMNQLYDRIEAALRQSLKIAQAGGAWPGDPVADANVLLAFV
                     VGRWTLFAKSGFKRLPQDGWDAQRKYLFA"
     gene            complement(1716..2609)
                     /gene="argB"
                     /locus_tag="VHT22_00590"
     CDS             complement(1716..2609)
                     /gene="argB"
                     /locus_tag="VHT22_00590"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX3136072.1"
                     /translation="MKSVSIEVGSSALKAQILAEALPYIQRFHDKAIVIKYGGNAMTE
                     PALKAGFARDVVMLKLVGMNPVIVHGGGPQIGDLLKTMGIHSEFRQGMRVTDDKVMNV
                     VEMVLGELNQEIVGLINQHGGRAVGLTGQDGAFIHARKLLLKSETNHGEFVDIGLVGE
                     IEKIDPELINLLDSRDFIPVIAPIGVGHEGEAYNINADLVAGKLAETLNAEKLVLMTN
                     TAGVLDKHGNLLTGLSASEIEALFADGTIHGGMLPKIGSALDAVKNGVKSSHIIDGRV
                     EHALLLEVLTNEGVGTMISAD"
     gene            2748..>3472
                     /gene="rfaQ"
                     /locus_tag="VHT22_00595"
     CDS             2748..>3472
                     /gene="rfaQ"
                     /locus_tag="VHT22_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipopolysaccharide heptosyltransferase
                     III"
                     /protein_id="HEX3136073.1"
                     /translation="MPSPLPDPVDLGLVHRALVTKLRHHGDVLLASPVFTVLKRAAPA
                     MEIDALVYAETAAMLAGHPAITETHVIDRDWKRQGFARQARAERALLSALSKRRYDLI
                     VHLTEHPRGAWLSRLLRPRYSVARHLDRAHWLWRTSFTHYYRLPRRTPRHTVECNLDS
                     LRRIGVQPAPGDRALVLAPNSAAAARVRGLLAQHRIEPRRFIQLHPGSRWLFKCWPAE
                     HYTALLEQLTSDGWRIALTGAPDP"
BASE COUNT          546 a         1188 c         1093 g          645 t
ORIGIN      
        1 tctgcagcgc cagatcctgt ttggcaccgc cgacgtcggc cggcccaagg tcgacgccgc
       61 ggccgagcgc attgccgcgg tcaatccgga agtgcgagtc gagcgcgtcg ccaggcgcgt
      121 cggcccgcag gatctcgcgc cgctcgcggc cgaggcggat atcgttctcg actgttccga
      181 caacttcgcc acgcgccatg cgatcaaccg cgcctgcgtc gccgcgaaaa agccgttggt
      241 ctccggcgcc gcgatccgct tcgacgggca gatcgcggta ttcgacgcgc gcgatggcag
      301 ccatccctgc tatcactgtc tgttcggcga gggtagcgac ttcgaggaga cacgctgcgc
      361 ggtcatgggc gtgttcgcgc cgctggtggg catcgtgggc gcaacgcagg ctgccgaagc
      421 gctgaagctg atcgccccgg cgggccgctc gctcgccggc cgcctgctgc tcgtcgatgc
      481 gctgacgatg gaatggcgcg aagtccgggt gctgaaggat ccggcgtgtc cggtgtgcgg
      541 cacgccggcg cgatgagggg cgtcgtgccg gcgcgatgga gcctcagcgg tgcgatgacg
      601 cgactcgccg tcgtggggat cgcctgctgg ctgggcgttg cggctgccgc gcaaaccgca
      661 ccgacggccg cggaatggca ggcgatccag caagtcatcg ccgcacagcg ggcggcgatc
      721 atcgccggcg aggcggaaaa ggcgttcagc tatgcttcgc ccggcatcca gcagcagttc
      781 ggcgatgccg caagcttcct cgcgatggtc gatgcggctt acgccgcgct ggagtcggcg
      841 cgctacgtgg aatttctgga aggcgccgtt atcgagggcc tggtcgtgca gccgctgcgc
      901 ctgatcgacg cggacaacac cgtgcgcgtc gcgttgtaca cgatggaaaa acaggccaac
      961 ggcgaatggc ggatctcgag ctgccggatc gccccgtcga ccgtgcgcgc ggcgtaggtc
     1021 tggggccttg cgctttaagc attaagcgaa cagatacttg cgctgcgcgt cccagccgtc
     1081 ctgcggcagg cgcttgaatc cggatttggc gaacagcgtc cagcgcccga cgacaaacgc
     1141 gagaagaacg ttcgcgtcgg cgaccgggtc ccccggccag gcgccgcccg cctgcgcgat
     1201 cttcagcgac tggcgcagcg cggcctcgat ccggtcgtac agctggttca tgcgcgcctg
     1261 caggcgttca tcctcattga ccagcgcatc gccgatcagc acgcgcgtca tgcccggatt
     1321 cttttcggcg aacccgagca gcacggcaac catctgcgtc gcctgcgcga cgccgtcggt
     1381 cgactcgctg ctgatgcgat tgaccagcga gaacagcgtt gtctcgatga actcgatcag
     1441 cccctcgaac atttgcgcct tgctggcgaa gtggcgatat aacgccgcct cggagacatc
     1501 gaggcgactc gccagcgcgg cggtcgtcac cttctcgcct cgcggcgtct ccagcatggc
     1561 ggccagcgtc tgcaggatct gcagccgccg ttctcccggt ttgctcgcca ttctcccgct
     1621 ccctgatcaa atcggcggca tcgcttcgtg acgcccgatg ctgccgaatc gcgactttag
     1681 cttcgccacg accgttcgct gcctgcgtgg atcgattagt ccgcgcttat catcgtcccg
     1741 acgccctcgt tggtcagcac ctccagcagc agcgcgtgct cgacgcggcc gtcgatgatg
     1801 tgcgaactct tgacgccgtt ttttaccgcg tccagcgccg agcctatctt cggcagcatg
     1861 ccgccgtgga tcgtgccgtc ggcgaacagc gcctcgatct cgctggccga aagcccagtc
     1921 agaagattgc cgtgcttgtc gagcacgccg gcggtgttgg tcatcagcac cagcttttcg
     1981 gcattcagcg tctcggcgag cttgccggca acgaggtctg cattgatgtt gtacgcctcg
     2041 ccttcgtggc ctacgccaat cggcgcgatc accgggatga agtcgcgcga atcgagcaga
     2101 ttgatcagtt ccggatcgat cttctcgatt tccccgacca gcccgatgtc gacgaattcc
     2161 ccgtggttgg tttcgctctt cagcagaagc ttgcgggcgt gaatgaacgc gccgtcctgg
     2221 ccggtcaggc cgaccgcgcg tccgccgtgc tggttgatca gcccgacgat ctcctggttg
     2281 agctcgccca ggaccatctc tacgacgttc atcaccttgt catcggtaac gcgcatgcct
     2341 tggcggaact cgctgtggat gcccatcgtc ttcaacaggt cgccgatctg cgggcccccg
     2401 ccgtgcacga tcaccggatt cattccgacc agcttcagca tcaccacgtc gcgcgcgaat
     2461 cccgccttga gtgctggctc ggtcatcgca ttgcccccgt acttgatgac gatcgccttg
     2521 tcgtgaaaac gctggatata aggcagcgcc tcggccagga tttgggcttt cagggcggac
     2581 gagccgacct cgatcgagac ggatttcatc agacgcttcg ggttgcggcg attgtacttg
     2641 aaggcgcccg aatagaatac gagcgccgct gtgccaacga gtaagcccta actggcgaag
     2701 cgctgtccgc gtttgtatcg aacaaggcat tttgctatct tgcctccatg ccgagcccgc
     2761 tgccggaccc cgtcgacctc ggtcttgtcc accgcgcgct ggtcaccaag ctccgccacc
     2821 acggcgacgt tctgcttgct tcaccggtct ttaccgtact caagcgcgcc gctccggcaa
     2881 tggaaatcga tgcgctggtg tacgcggaaa ccgcagcgat gctcgccggc catccggcga
     2941 tcaccgaaac gcatgtcatc gatcgcgact ggaaacggca gggatttgcc cgccaggcgc
     3001 gcgccgagcg tgcgctgctg tcggcactct cgaagcgtcg ctacgatctc atcgtccacc
     3061 tgaccgagca tccgcgcggc gcttggctct cgcgcctgct gcgtccgcgc tacagcgtcg
     3121 cgcgccatct cgatcgcgcg cactggctgt ggcgaacgag cttcacccac tattaccggc
     3181 tgccgagacg gacgccgcgc cacaccgtcg aatgcaatct cgacagcctg cgtcgcatcg
     3241 gcgtgcagcc tgcgccgggc gacagagcgc ttgtcctcgc gccgaacagc gccgcggcgg
     3301 cgcgcgtgcg cggcctgctc gcccagcacc ggatcgagcc caggcgtttc atccagctgc
     3361 accccggttc tcgctggctg ttcaaatgct ggcccgccga gcattacacg gccttgctcg
     3421 agcagctcac ttccgacggg tggcgcatcg cgctgaccgg ggcgccggac cc
//
LOCUS       DATANS010000042         4058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_548055, whole genome shotgun sequence.
ACCESSION   DATANS010000042 DATANS010000000
VERSION     DATANS010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548055"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VHT22_00600"
     CDS             complement(<1..629)
                     /locus_tag="VHT22_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX3136074.1"
                     /translation="MVALVTSAPQAASDDLYAARVAQAASGYTATERTAIEATFELAR
                     KRYGNALTAEGEPWLARATGTATIVAELKLDAASVCAALLIGVPDSAGFDEQKFAVEA
                     GDEVAKMVTGVARMGAIRTVSLGQGGAHKEARDEQAENLRKMLLAMVEDIRVVLVKLA
                     ERTQAMRHLVAAPPDAPDARGTAAARATQRNSQARETQDLFAPLANRLGV"
     gene            complement(701..2062)
                     /gene="glmM"
                     /locus_tag="VHT22_00605"
     CDS             complement(701..2062)
                     /gene="glmM"
                     /locus_tag="VHT22_00605"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX3136075.1"
                     /translation="MTKNYFGTDGVRGRVGDSPITPELVLRLGYAAGRVFAAESAARP
                     GTTPAVLIGKDTRISGYLLEAALEAGLSAAGVDVYLSGPLPTPGVAYLTRALRLSAGI
                     VISASHNPFEDNGIKFFSAGGTKLPDAVERAIEQGIDGPISCVKSAELGKAFRVNDAS
                     GRYIEFCKGTFPNDLDLKGLKIVVDCAHGAGYHVAPPVFHELGAEVVAVGNEPDGINI
                     NAGVGATHPEYLQAAVLEHGADLGVALDGDGDRLMMVDADGRIYDGDQLLYVIALDYW
                     RRGATAGGVVGTLMSNLGFEQALDRRGIPLQRSAVGDRYVLEMMQDKGWLLGGENSGH
                     IICLDKHTTGDAIVAALAVIRALREQQTTLAAATADAPLFPQRLINVPIRRGFDWRGS
                     EPIRRAEREAMASLDGSGRVLLRPSGTEPVLRVMVEAREAAAADRCARGIADAVATAV
                     ASS"
     gene            complement(2131..2949)
                     /gene="folP"
                     /locus_tag="VHT22_00610"
     CDS             complement(2131..2949)
                     /gene="folP"
                     /locus_tag="VHT22_00610"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX3136076.1"
                     /translation="MGVLNVTPDSFFDGGRYADRHRALGHAHQMLDDGAAIIDIGGES
                     TRPGATPTAETEELDRVLPLLEALRADCDARGVPLSVDTRKPAVMRAAIAAGASMVND
                     INALRADGALDAVAKSGAAVCLMHMQGEPLTMQKSAVYDDDVVAVVAAFLSERALACE
                     RAGIARERIAVDPGFGFGKTIDHNIELLRRLSEIVALGYPVVAGLSRKSTIGTLTGRR
                     VEERGAGSLAAALAAVARGASFVRVHDVRDTVDALKIWQAVQADRRADHASGSA"
     gene            complement(3065..>4058)
                     /locus_tag="VHT22_00615"
     CDS             complement(3065..>4058)
                     /locus_tag="VHT22_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HEX3136077.1"
                     /translation="ATNRPDVLDPALMRPGRFDRQVVVPLPDIRGREQILLVHMRKVP
                     VAGDVKAEVIARGTPGFSGADLANLVNEAALFAARKSKRLVDMDDFEMAKDKIMMGAE
                     RKSMVMSEEEKKNTAYHESGHTVVAKLLPKSDPVHKVTIIPRGRALGVTMQLPEADRY
                     SFDRDYLLQRIAVLFGGRISEEIFMHQMTTGASNDFERATQMARDMVTRYGMSDTLGP
                     MVYAENEGEVFLGRSIATQKNVSEATMQKVDLEIRRIIDEQYALARKLIEENRDKVEA
                     MAKALLEVETLDSDQVEDIMQGRPPRPPKPVSATPGTTFGPDTGPDAAPSANPA"
BASE COUNT          604 a         1411 c         1373 g          670 t
ORIGIN      
        1 acgccgagcc ggttggcgag cggcgcgaac agatcctgcg tctcgcgtgc ctgcgaattg
       61 cgctgcgtcg cgcgcgccgc ggcggtgccg cgcgcatccg gcgcgtcggg cggtgctgcg
      121 accaggtggc gcatcgcctg cgtgcgctcg gcgagcttca ccaggacgac gcggatgtcc
      181 tcgaccatcg ccagcagcat cttgcgcagg ttctcggcct gctcgtcgcg cgcctccttg
      241 tgcgcgccgc cttggcccag agacacggtg cggatcgcgc ccatgcgcgc gacgccggtg
      301 accatcttcg cgacttcgtc gcccgcttcg acggcgaact tctgctcgtc gaagccggcg
      361 ctgtccggca cgccgatcag cagcgcagcg cacaccgatg cggcgtcgag cttgagctcc
      421 gcgacgatcg tcgcggtgcc ggtcgcccgg gcgagccagg gttcgccttc cgcggtcagc
      481 gcatttccat agcgcttgcg cgcaagctcg aacgtcgcct cgatcgccgt ccgctcagtg
      541 gccgtgtatc cgctcgcagc ctgcgcgacg cgcgccgcgt agaggtcgtc ggacgcggct
      601 tgcggcgcag aagtaacgag cgcaaccatg acgtcgatta taccggcgcg tgcactccgt
      661 tcttagcgcg ggaaatgctg agcttagcgc ccgggcgacg ttagctcgac gctaccgcgg
      721 tcgcgaccgc gtcggcgatg ccgcgcgcgc agcggtcggc ggcggcggct tcgcgcgcct
      781 cgaccatcac ccgcagcacc ggctcggtgc ccgaaggccg caagagcacg cggcccgagc
      841 catcgagact cgccatcgct tcgcgctcgg cacggcgaat cggctcgctg ccgcgccagt
      901 cgaagccgcg gcggatcggc acgttgatca gccgctgcgg aaacagcggc gcatcggcgg
      961 tcgcggcagc aagcgtcgtc tgctgctcgc gcagcgctcg gatgacggca agcgccgcga
     1021 cgatcgcgtc cccggtcgtg tgcttgtcga gacagatgat gtgcccggag ttttcgccgc
     1081 ccaggagcca tcccttgtcc tgcatcattt ccagcacgta gcggtcgccg acggcgctgc
     1141 gctgcagcgg aatgccgcgg cggtcgagcg cctgctcgaa tccgaggttg ctcatcagcg
     1201 tgccgacgac gccgccggcg gtcgcgccgc gccgccagta gtcgagcgcg atcacgtaca
     1261 gcagttgatc gccgtcgtag atgcgcccgt cggcgtcgac catcatcaag cggtcgccgt
     1321 cgccgtcgag cgccacgccc agatcagcgc cgtgctcgag cacggcagcc tgcagatatt
     1381 ccggatgcgt cgcaccgacg ccggcgttga tgtttatgcc gtcgggctca ttgccgaccg
     1441 cgaccacctc cgcgccgagc tcgtggaaca ccggcggagc gacgtgatag ccggcgccat
     1501 gggcgcaatc gacgacgatc ttcaagccct tgaggtcgag atcgttcgga aacgtgccct
     1561 tgcagaattc gatgtagcgt cccgatgcgt cgttgacgcg gaatgccttg cccagctccg
     1621 ccgacttgac gcagctgatc ggaccgtcga tgccctgctc gatcgcacgc tcgaccgcgt
     1681 ccggcagctt ggtgccgccg gccgagaaga acttgatgcc gttatcctcg aacggattgt
     1741 gcgaagcgct gatcacgatg ccggccgaca ggcgcagcgc gcgcgtcagg taggcgacgc
     1801 caggcgtggg cagcggaccg gacagataga cgtcgacgcc ggcggcggag agccccgctt
     1861 cgagcgccgc ctcgagcagg tagccggaga tgcgcgtgtc cttgccgatc agcaccgccg
     1921 gcgtcgtgcc cgggcgcgcc gccgattcgg ccgcgaacac gcggccggca gcgtaaccga
     1981 gccgcagcac gagctccggc gtgatcggcg agtcgccgac tctgccgcgg acgccatcgg
     2041 tgccgaaata gttcttggtc atggtcgaac gctcatttgc cgccagtcag tgcactcacg
     2101 cagtcgccac tcggtacagc cattatgaac tcacgcgctt ccgcttgcgt ggtcggcacg
     2161 tcggtctgcc tgcaccgcct gccagatctt gagcgcgtcg acggtgtcgc gtacatcgtg
     2221 tacgcgcacg aacgacgcgc cgcgggcgac ggccgcgagc gccgccgcca ggctgccggc
     2281 gccgcgctcc tcgactcggc gcccggtgag ggtgccgatc gtcgacttgc gcgagaggcc
     2341 ggcgacgacc gggtagccga gcgcgacgat ctcggaaagc cgccgcaaca gctcgatatt
     2401 atggtcgatc gtcttgccga aaccgaatcc cggatcgacg gcgatgcgtt cgcgcgcgat
     2461 tccagcgcgt tcgcaggcca gcgcacgctc ggaaagaaac gcggccacga ccgcgacgac
     2521 gtcgtcgtca tacacggcgc ttttctgcat cgtcagcggc tcaccctgca tgtgcatcag
     2581 gcagaccgcg gcgccgctct tcgcgaccgc gtcgagcgca ccgtccgcgc gcagcgcatt
     2641 gatgtcgttg accattgagg ctccggcggc gatcgcggcc cgcatcaccg cgggcttgcg
     2701 cgtgtcgacc gacagcggca cgccgcgcgc gtcgcaatcg gcgcgcaagg cctcgagcaa
     2761 cggcagcacg cgatcgagct cttcggtttc cgcggtcggc gtcgcgccgg ggcgcgtcga
     2821 ttcgccgccg atgtcgatga tcgccgcgcc gtcgtcgagc atttgatggg catggcccag
     2881 cgcgcgatgg cggtcggcgt accgaccacc gtcgaagaag gaatcgggcg tgacgttgag
     2941 cacacccatg acgcgcgggc agtccagcgc gacccggtgc cggccgcaga ccagatgccg
     3001 ggtggcaagc tccggcaagg gccgacgaac tgctctcgtg agccctgcgc ggccgcgaat
     3061 gcggctaggc cgggttcgcc gagggcgcgg cgtcgggacc ggtgtcgggc ccgaacgtgg
     3121 tgcccggcgt ggcgctcacc ggcttcggcg gacgcggcgg acggccctgc atgatgtcct
     3181 cgacctggtc gctgtcgagc gtttcgactt cgagcagcgc cttggccatc gcttcgacct
     3241 tgtcgcggtt ttcctcgatg agcttccgcg ccagcgcata ctgctcgtcg atgatgcgac
     3301 ggatctccag gtcgaccttc tgcatcgtcg cctcggacac gttcttctgc gtcgcgatcg
     3361 agcggccgag gaacacctcg ccctcgttct cggcgtacac catcgggccc agcgtgtcgg
     3421 acatgccgta gcgtgtgacc atgtcgcgcg ccatctgcgt cgcgcgctcg aagtcattgc
     3481 tcgcaccggt ggtcatctgg tgcatgaaga tttcctcgga gatgcggccg ccgaacagca
     3541 cggcgatgcg ctgcaggagg taatcgcgat cgaaggaata gcggtcggcc tcggggagct
     3601 gcatggtcac gcccagcgcg cgcccgcgcg ggatgatcgt caccttgtgc accggatcgg
     3661 atttcggcaa cagcttcgcg acgaccgtgt gaccggactc gtgataagcc gtgttcttct
     3721 tttcctcttc ggacatgacc atcgatttgc gctcggcgcc catcatgatc ttgtccttgg
     3781 ccatttcgaa atcgtccatg tcgaccaggc gcttgctctt gcgcgcggcg aacagggccg
     3841 cttcgttgac cagattggcg agatcggccc cggaaaagcc cggcgtgccg cgcgcgatga
     3901 cctcggcctt gacgtcgccg gcgaccggca ccttgcgcat gtgcaccagc aggatctgct
     3961 cgcggccgcg gatgtcgggc agcggcacga ccacctgacg gtcgaagcgg cccggacgca
     4021 tcagtgccgg gtcgagcacg tccggccggt tggtcgcg
//
LOCUS       DATANS010000043         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_2251, whole genome shotgun sequence.
ACCESSION   DATANS010000043 DATANS010000000
VERSION     DATANS010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2251"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /gene="gatB"
                     /locus_tag="VHT22_00620"
     CDS             complement(<1..910)
                     /gene="gatB"
                     /locus_tag="VHT22_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019717332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX3136078.1"
                     /translation="MTWEVVIGLETHAQLSTASKIFSGASTAFGAAPNTQASAVDIAL
                     PGVLPVLNRGAVERAIRFGLAVGAAINRKSVFARKNYFYPDLPKGYQISQYEIPVVQG
                     GSVAIVSPTRGEIRIALTRAHLEEDAGKSLHERIASPGTPGHEMTGIDLNRAGMPLLE
                     IVSEPDMRSAAEAVVYAKALHALVRWIGICDGNMQEGSFRFDANVSVRRQGEAKLGTR
                     CEIKNLNSFRFLQQAIEFEVRRQTELIEDGGKVVQETRLYDPDRGETRSMRSKEDAQD
                     YRYFPDPDLPPLSIDEAWIERMRADMP"
     gene            complement(972..>2142)
                     /gene="gatA"
                     /locus_tag="VHT22_00625"
     CDS             complement(972..>2142)
                     /gene="gatA"
                     /locus_tag="VHT22_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX3136079.1"
                     /translation="FVVEQLAAAGMVLVGKTNMDEFAMGSSNENSFFGPVRNPWNADF
                     VAGGSSGGSAAAIAARFVPAATGTDTGGSIRQPAALSGVCGIKPTYGVCSRYGLVAFA
                     SSLDTPGVFGQTAADCAMLLTAMAGHDARDSTSLDRPREDYARDIDAAVTSKPLAGLR
                     IGLPREYAGEGTDVAVARAIETALAEFRRLGAVTVDVSLPNVHLSVPVYYVIAPAEAS
                     SNLSRFDGVRYGHRAASYSDLDDMYCKTRAEGFGAEVKRRILVGTYVLSHGYYDAYYL
                     KAQQVRRLIADDFRRAYDSCDLIIGPTSPTAAFRLGEKSDDPVKMYLNDIFTIAGNLT
                     GAPAMSIPCGFDERGLPIGLQIQGDHFAEAKILNAAHRYQQVTDWHRRIPTEFAP"
BASE COUNT          349 a          711 c          700 g          382 t
ORIGIN      
        1 cgggcatgtc ggcgcgcatc cgttcgatcc atgcctcgtc gatggacagc ggcggcagat
       61 cgggatcggg aaagtaacgg tagtcctgcg cgtcctcttt gctgcgcatc gagcgtgtct
      121 cgccgcggtc agggtcgtac aggcgcgttt cctgcacgac cttgccgccg tcctcgatca
      181 actcggtctg ccggcgcacc tcgaactcga tcgcctgctg cagaaaacga aagctgttca
      241 gattcttgat ctcacaccgc gtgccgagct tcgcctcgcc ttgccgccgc accgagacgt
      301 tggcgtcgaa gcggaaactc ccttcctgca tgttgccgtc gcagatgccg atccagcgca
      361 ccagcgcatg cagggccttg gcgtaaacga cagcctcggc ggccgagcgc atgtccggct
      421 cggagacgat ttcgagcaac ggcatgccgg cgcggttgag atcgatgccg gtcatctcgt
      481 ggcccggtgt tccgggcgac gcgattcgct cgtgcagcga ttttccagcg tcctcctcca
      541 gatgcgcgcg cgtcagcgca atccggatct cgccgcgggt cggcgacacg atcgcgaccg
      601 atccgccctg cacgaccggt atttcgtatt gcgagatctg gtagcccttc ggcaggtcgg
      661 ggtagaagta gttcttgcgc gcgaacacgc tcttccggtt gatcgctgcg cccaccgcca
      721 ggccaaagcg gatcgcacgc tcgaccgcgc cgcggttcaa taccggcagg acaccgggca
      781 gcgcgatatc gacggccgaa gcctgcgtgt tcggggcggc gccgaaggcc gtcgacgcgc
      841 cagaaaatat cttcgatgcg gtcgacagtt gcgcatgcgt ctcgagaccg atgacgacct
      901 cccaggtcat tggactaccc tccgcgctgc gcgcttcggg gctgattcgc gctcggggcg
      961 gcccttcgcg ctcatggcgc gaactccgtc ggaattcggc gatgccagtc ggtgacctgt
     1021 tgatagcgat gcgcggcgtt gagaattttc gcctcggcaa agtgatcgcc ctggatctgc
     1081 agcccgatcg gcaaacccct ctcgtcgaaa ccgcagggaa tcgacatcgc cggcgccccg
     1141 gtcaggttgc cggcgatggt aaaaatgtcg ttcaggtaca tcttgaccgg atcgtcgctt
     1201 ttttctccga gccggaacgc cgcagtcggc gatgtcggcc cgatgatcag gtcgcaactg
     1261 tcgtaggcgc gacggaaatc gtccgcgatc agccggcgga cctgctgcgc tttcaggtag
     1321 tacgcgtcgt agtagccgtg cgagagcacg taggtcccga ccaggatgcg gcgcttgacc
     1381 tcggcgccga agccttcggc gcgggttttg cagtacatgt cgtcgaggtc gctgtagctc
     1441 gccgcgcgat gcccgtagcg cacgccgtcg aagcgcgaca ggttggacga ggcttcggcg
     1501 ggtgcgatta catagtagac ggggaccgac aggtggacgt tgggaaggct gacgtcgacc
     1561 gtgactgcgc cgaggcggcg gaattctgcg agcgcggtct cgatcgcccg cgccacggcg
     1621 acgtcggtcc cctctcctgc gtactcgcgc ggcaagccga ttcgcagccc cgcgagcggc
     1681 ttgctcgtca cggccgcgtc gatgtcccgc gcgtagtcct cccgcggccg gtcgaggctg
     1741 gtcgagtccc gcgcatcgtg gccggccatc gcggtaagca gcatcgcgca gtcagcggcc
     1801 gtctggccga agacgccggg cgtgtcgagg ctcgaggcga aagccacgag accatagcgc
     1861 gagcagacgc cataggttgg tttgatgccg cacacgcccg acagcgccgc cggctgtcga
     1921 atcgatcccc cagtgtcggt gccggtcgcc gcgggaacga agcgcgccgc gatggccgcc
     1981 gccgatccgc ccgagctccc cccggccaca aagtcggcgt tccatgggtt cctgaccggg
     2041 ccgaaaaagg aattttcgtt cgacgagccc atcgcgaact cgtccatgtt ggtcttgccg
     2101 accagcacca tgcccgcggc cgccagctgc tcgacgacaa ag
//
LOCUS       DATANS010000044         3891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_422406, whole genome shotgun sequence.
ACCESSION   DATANS010000044 DATANS010000000
VERSION     DATANS010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422406"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VHT22_00630"
     CDS             <1..434
                     /locus_tag="VHT22_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013650549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3136080.1"
                     /translation="VGAMLTTEEIASSFSLGVHGTTYGGNPLACAVAGAVFDVINTVE
                     VLDGVKARHALFVEGLRTINGRREVFADIRGEGVWIGCELSPHWHGRAADVMRAASDA
                     GLIVLMAGPDVVRIAPSLVISLDEILEGLARLETALGKALN"
     gene            493..1536
                     /gene="astA"
                     /locus_tag="VHT22_00635"
     CDS             493..1536
                     /gene="astA"
                     /locus_tag="VHT22_00635"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HEX3136081.1"
                     /translation="MFTFRPIEQADLPGLFALSEKSGTGLTSLPANAERLGSRIERSL
                     ASFAGNAASADACYVFVLIDSATQGVVGISAIEAAVGLAEPWYNYHVGTQVHASRELG
                     VYTSAPTLFLSNDHTGNSELCSLFLDARYRHGKNGPLLAKARLLFIAEFADRFAPKII
                     AEMRGRLDADGKSPFWEGLGRHFFTIEFSRADYLTGIGQKAFIAELMPRHPVYTTLLP
                     ADARAAIGEVHADTMPARAMLESEGFRYEGYVDIFDAGPTLECFRDDIHAVRQSQILP
                     VSVGEADPVPDSLSEDVLWLVASRNFTAFRAILCPAPARIAQFPLLPHAAEALGVRAG
                     DSVRAVPLSPRDR"
     gene            1571..2980
                     /locus_tag="VHT22_00640"
     CDS             1571..2980
                     /locus_tag="VHT22_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX3136082.1"
                     /translation="MTQPHILILMTDQQRADCMSCAGHAQLKTPHMDRLAAEGMRYTQ
                     ATTVSPICMPARASFASGLYPHNHGMWANQGELPAEDETFFQLLQSVGYCTAQVGKAH
                     FYQHQVGVDMRSREPYMHARGLEYVHEIPGPKVAGRMISYMAEEWRAKGLLERYVEDY
                     RERVRSETGGVWPSPLPVEDFLDSYVGRKAVEFVERYEDRRPMCLFVGFGGPHEPWDA
                     PGQYAEMYRPEDTAKPTPIPDEYAVLPDALKAKRDFAVLPDAILGNVANIRANYYGKI
                     SLIDDNIGYILEAFEKRGWLDDLFVIFLSDHGEMLGDHGRIRKSTFHEASVRIPLILR
                     WPGRIPANEISEALVEIVDIFPTLVEAGEGKPSARCLGRSLWPLIHKERGELKEWQVS
                     EIVYGEPRIMLRSRRWKLAIDARGAAFMLYDLEHDPEEQHNLAPVQAARAVQLQLSES
                     LASRLKELRYGQRVSPGAA"
     gene            3019..>3891
                     /locus_tag="VHT22_00645"
     CDS             3019..>3891
                     /locus_tag="VHT22_00645"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HEX3136083.1"
                     /translation="MPPSDRAAVEANFDGLPGPTHNYSGLATGNLASERNRSAIANPR
                     LAALQGLAKMKALAGRGYPQAVLPPHERPDVAALRSLGFTGDDATIIARAARDHAPLL
                     AACSSAAAMWAANAATVSPSADTADRRVHFTPANLMSHFHRAIEAPTTTRVLRAVFAD
                     ESRFAVHDPLPAAAELGDEGAANHTRFASDPAAPGIELFVYGRVAWATAASAPSRFPA
                     RQTREASQAIARRHGLDPARTLFAQQSPAAIDAGVFHNDVIAVGHHNVMFCHQRAWLD
                     QNAVLAALAQGVGPA"
BASE COUNT          695 a         1277 c         1258 g          661 t
ORIGIN      
        1 ccgtaggggc gatgctgacc accgaggaaa tcgcgagctc gttttcgctc ggcgtgcacg
       61 ggacgaccta cggcggcaac ccgctggctt gcgcggtggc cggcgcggtg ttcgacgtga
      121 tcaacactgt cgaggtgctc gacggcgtca aggcgcggca cgcgctgttc gtcgaggggc
      181 tgcgcacgat caacggccga cgcgaggtct tcgccgacat ccgcggcgaa ggtgtctgga
      241 tcggctgcga gctttcgccg cactggcacg gcagggccgc cgatgtaatg cgcgcagcga
      301 gcgacgcagg gctgatcgtg ctgatggccg gtcccgacgt cgtccgcatc gccccgtcgc
      361 tggtgatcag cctcgacgaa atcctcgagg gactggcacg gctcgaaacc gcgctgggca
      421 aggcgctcaa ctaaagcttg gcggcggccg gcggcgcacg gtgacgcgaa tgccagattc
      481 cgccgcgcgc tgatgttcac cttccgaccg atcgagcaag cggatctgcc tgggctgttc
      541 gcgctgtcgg aaaagagcgg cacggggctg accagcttgc cggccaacgc cgagcggctg
      601 ggctcgcgca tcgagcgctc tctcgcgtca ttcgcgggca atgccgccag cgccgatgcc
      661 tgctatgtgt tcgtgctgat cgacagcgcg acgcaaggcg tcgtcggcat cagcgcgatc
      721 gaggcagcgg tcgggctcgc ggagccctgg tacaactacc atgtcggcac gcaggtgcat
      781 gcctcgcgcg agctcggcgt ctataccagc gcgccgacct tgttcctgag caacgatcac
      841 accgggaaca gcgagctgtg ctcgctgttt ctggatgcgc gctatcgcca tggcaagaac
      901 gggccgctgc tcgccaaggc gcgcctgttg ttcatcgccg agttcgccga ccgcttcgcc
      961 ccgaagatca tcgccgaaat gcgcgggcgc ctcgacgccg acggcaagag cccgttctgg
     1021 gaaggcctgg gccgccattt cttcaccatc gagttttcgc gcgcggacta tctgaccggc
     1081 atcggccaga aggcattcat cgccgagttg atgccgcgtc acccggtgta cacgacgttg
     1141 ctgccggcgg acgcgcgtgc ggcgatcggc gaagtccacg ccgatacgat gccggcgcgc
     1201 gcgatgctcg aatcggaagg attccgctac gagggttacg tcgacatctt cgatgcgggc
     1261 ccgacgctcg agtgctttcg cgacgacatc catgcggtgc ggcaatcgca gatcctgccg
     1321 gtcagcgtcg gcgaggcgga cccggtgccg gacagcttga gcgaagacgt tctgtggctg
     1381 gtcgcgagcc gcaatttcac cgcattccgc gcgatcctct gcccggcgcc ggcgcgcatc
     1441 gcgcaatttc cgctgttgcc gcatgcggcc gaggcactcg gtgtccgcgc cggcgactcg
     1501 gtgcgcgcgg ttcccctctc gccgcgcgac cggtgactcg cccgggcggc caacccgtat
     1561 tatcgagcgc atgacgcaac cgcacatact gattctcatg accgaccagc aacgtgccga
     1621 ttgcatgagc tgcgcgggtc atgcacaact caagacgccc cacatggatc gcctggccgc
     1681 ggagggcatg cgctacaccc aggcaaccac ggtatcgccg atctgcatgc cggcacgggc
     1741 atcgtttgcc agcggtctgt atccccataa ccatggcatg tgggcaaacc agggcgaact
     1801 cccggcagag gacgagacgt tctttcagct gctgcagagc gtcggctact gcacggcgca
     1861 agtcggcaag gctcactttt atcaacatca ggtgggagtc gatatgcgca gccgcgagcc
     1921 ctacatgcat gctcgcggcc tggagtacgt tcatgaaatc cccggaccca aggtagcggg
     1981 tcggatgatt tcctacatgg ctgaagagtg gagagcaaag ggtctgctgg aaaggtatgt
     2041 cgaggactac cgcgagcgcg ttcggtcgga aaccggcggc gtatggccaa gtcctcttcc
     2101 ggtcgaggac ttcctggaca gctatgttgg tcgcaaggca gtcgaattcg tcgaacgcta
     2161 cgaagaccgc cggccgatgt gcctttttgt cggctttggc gggccgcacg aaccatggga
     2221 tgcgcctgga cagtatgccg agatgtacag gccggaggac accgccaaac cgacgccgat
     2281 ccccgacgag tacgctgtac ttccagacgc actgaaggcg aagcgagact ttgccgtgct
     2341 gcccgacgcg atcctgggca atgtggcgaa catcagggca aattactatg gaaaaatctc
     2401 gctcattgac gacaacatcg gctacatcct ggaggcgttt gaaaaaaggg gctggctcga
     2461 tgacttgttt gtcattttcc tgtcagacca cggcgaaatg ctcggcgatc atggacgtat
     2521 cagaaaatcg acgtttcatg aagcaagcgt gcgaatccca ctcatcctcc gctggccggg
     2581 cagaattccg gccaacgaga tcagcgaagc attggtggaa atcgtcgata tttttccaac
     2641 tctcgtcgag gctggcgaag gaaaaccctc ggcgcgatgt ctgggccgct cactgtggcc
     2701 gctgatccac aaagaacggg gtgagctaaa agagtggcag gtgagcgaaa tcgtatacgg
     2761 cgaaccccgc atcatgcttc gatcccggcg ctggaagctc gcgatcgatg cgcgtggcgc
     2821 tgcattcatg ctttatgatc tggagcacga tccggaggag cagcataacc tggcgcctgt
     2881 tcaggcagcc cgggccgtgc aacttcagtt gagcgaatct cttgcatcaa gactgaagga
     2941 actccgctac ggtcaacgcg tgtccccggg cgccgcatga attcgaccat ctgctgacgg
     3001 gagatcgcaa tcgcgcggat tccgccaagc gatcgggccg cagtcgaagc caacttcgac
     3061 ggcctgcctg gcccgacgca taactacagc gggctcgcga ccggtaacct ggcgtcggag
     3121 cgcaaccgca gcgcgatcgc caacccgcgg ctcgccgcgc tgcagggact cgccaagatg
     3181 aaggcgctgg ccggccgcgg ctacccgcag gccgtcttgc caccgcacga gcggcccgat
     3241 gtggccgcgc tgcgctcgct cggcttcacg ggcgacgacg cgacgatcat cgctcgagcg
     3301 gcgcgcgatc acgcgccgct ccttgccgcc tgctcgtccg ctgctgcgat gtgggcagcc
     3361 aacgcggcca ccgtgagccc gtcggccgac accgccgacc gccgcgtgca tttcacgccc
     3421 gccaacctga tgtcgcattt ccatcgcgcg atcgaagcgc cgaccacgac gcgcgtgctg
     3481 cgcgcggtct ttgccgacga gagccgcttc gccgtgcacg atccgctgcc tgccgcggcg
     3541 gaactcggcg acgaaggcgc ggccaaccac acccgattcg cctcggaccc ggcggcgccc
     3601 ggcatcgagc tctttgtcta cggccgggtc gcctgggcga cggccgcgag cgcgccatcg
     3661 cgctttccgg cacgccagac gcgcgaagct tcgcaggcga tcgcgcggcg gcacggcctc
     3721 gacccggcgc gtaccttgtt cgcccagcag agcccggcag cgatcgacgc cggcgtgttc
     3781 cacaacgacg tgatcgcggt gggccaccac aacgtgatgt tctgccacca gcgtgcctgg
     3841 ctcgatcaga acgcggtgct ggcggcgctc gcgcaaggcg tcggtccggc c
//
LOCUS       DATANS010000045         2706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_548328, whole genome shotgun sequence.
ACCESSION   DATANS010000045 DATANS010000000
VERSION     DATANS010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548328"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="VHT22_00650"
     CDS             complement(<1..1205)
                     /locus_tag="VHT22_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3136084.1"
                     /translation="MFRFFERLVDPYPEATPETPPRGFFAFLWACSKGLRGYLLATTL
                     LTAVIGAFEALLFSMMGSIVDWLARVQPAELWAKEGGRLLLLGGVLAGSILLIAVQSM
                     VKQQTLFGNFPMLLRWNFHRLMLGQSMTFYQDDFAGRIATKVMQTALAVRDTWLIVCE
                     LLVFVVIYFVTLLAVLGNFDSRLIIPFLGWVVLYIAALYFFVPRLGRVARMQADARSL
                     MTGRMTDAYTNIATVKLFSHARREAFFARTAMQEFLNTVHTQMRLVTGFEIVNHTLSM
                     GLIATSAGATLWLWTQGQVGVGAVAAATAMALRLNGISHWVMWELSSLFEHIGTVQDG
                     INTIARRQTVVDAPEAKPITVAHGDIRFEQVSFAYGGKRDVIEKLDLHIRPGEKIGLV
                     GRSGAGKST"
     gene            1406..2068
                     /locus_tag="VHT22_00655"
     CDS             1406..2068
                     /locus_tag="VHT22_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3136085.1"
                     /translation="MKSPAPAPSTRDRLIQTAMQLFWEKGYGSTSVADVLHAAGVNSG
                     SLYHFFPGKQDLLLAVLDAYRAGIDPMLLAPAWKGVDDPIERVFALLARYRLGVVQTD
                     CVYACPIGSLALEIHEPDPPVRKALAANFKAWTDAVEACLVAAGPRLPRTLDRRELAR
                     FVLTAMEGGVMQARTHRDPAYFDSSVRQLRIHFEHLEREAAQQPSRARSPRKKSTSRG
                     SK"
     gene            2065..2580
                     /locus_tag="VHT22_00660"
     CDS             2065..2580
                     /locus_tag="VHT22_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136086.1"
                     /translation="MMRLKLRITLAGVLVSAAAFSGSAAAAPIEPLQPMAFLAGHCWK
                     GDFPGGKETDEHCFAWLYDGKALRDTHTVRAPGRPDYIGETTYYWDSAAKRVEFVYIE
                     NLGGISRGNMQSMPEALIFPPTQYVADGQAMTYRVRWTRLSDTSYEAWSEMQGKDGWT
                     TMFKLTMKRSS"
BASE COUNT          495 a          892 c          875 g          444 t
ORIGIN      
        1 atggtcgact tgcccgcgcc cgagcgtccg accaggccga tcttttcgcc cggacggatg
       61 tggaggtcga gcttttcgat cacgtcgcgc ttgcctccgt aggcgaagct cacctgctcg
      121 aagcggatgt cgccgtgcgc gacggtgatc ggcttcgcct ccggagcgtc gacgaccgtc
      181 tgccgccgtg cgatcgtatt gatgccgtcc tgcacggtgc cgatgtgctc gaacagcgac
      241 gacagctccc acatcaccca gtgcgagatg ccgttcaggc gcagcgccat cgcggtggcg
      301 gcggcgaccg cgccgacgcc gacctggcct tgcgtccaca gccagagcgt ggcgcccgcg
      361 ctggtggcga tcagtcccat cgacagcgtg tgattgacga tctcgaaacc ggtaaccagc
      421 cgcatctgcg tgtgcacggt gttcaggaat tcctgcatcg ctgtgcgcgc aaagaacgcc
      481 tcgcgccgcg cgtgcgaaaa gagcttgact gtcgcgatgt tggtgtaggc gtcggtcatg
      541 cgaccggtca tcagcgagcg cgcgtcggcc tgcatgcgcg cgacgcgccc cagccgcggc
      601 acgaagaagt acagcgcagc gatgtacagc acgacccagc caaggaaggg gatgatcagg
      661 cgtgagtcga aattccccag cactgcgagc aaggtgacga agtagatgac gacgaagacc
      721 agcagctcgc agacgatcag ccaggtgtcg cgcacggcga gcgcggtctg catcaccttg
      781 gtcgcgatgc ggccggcgaa gtcgtcctgg tagaacgtca tgctctgccc gagcatcagc
      841 cggtggaagt tccagcgcag cagcatcgga aagttgccga acagcgtctg ctgcttgacc
      901 atcgactgca cggcgatcag caagatgctg ccggcgagga cgccgcccag cagcagcagg
      961 cgccctcctt ccttcgccca gagctccgct ggttgcaccc gcgccagcca gtcgacgatg
     1021 ctgcccatca tgctgaacag cagcgcctcg aacgcgccga tcacggcggt caacaaggtc
     1081 gtggcgagca gatacccgcg gagtcccttc gagcacgccc acaggaacgc gaaaaaaccg
     1141 cgcggcggcg tctcgggggt cgcctcggga taaggatcga ccaggcgttc gaaaaagcgg
     1201 aacacggtgt cttgtcgtcg tttatggtcc ggcgctttag tgcgtcggac ggatcggcgg
     1261 aacggtggcg ccggcgcgaa gtcggccagc gacgcgccgg aaaagtggaa ttctactatt
     1321 gctccgcgcc aggtgctcgc gacgggcctt gcttgacaca tcggccgacg gcgtttactc
     1381 tcttagttga tcgttcaatc aactcatgaa aagccccgct ccggcgccct ccacgcgcga
     1441 ccgcctgatc cagacggcga tgcagctgtt ctgggagaaa ggctatggct cgaccagtgt
     1501 ggccgacgtc ctccacgcgg caggcgtgaa cagcggcagc ctgtatcact tctttcccgg
     1561 caagcaggat ctgctgctcg cggtgctcga cgcctaccgc gccggcatcg acccgatgct
     1621 gcttgcaccc gcgtggaagg gggtcgacga tccgatcgag cgcgtattcg cgctgctcgc
     1681 gcgctaccgg cttggcgtcg tgcagaccga ttgcgtctac gcctgcccga tcggcagtct
     1741 cgcgctcgag atccacgagc ccgacccgcc ggtgcggaag gcccttgccg cgaacttcaa
     1801 ggcgtggacc gacgccgtcg aggcctgcct cgtcgcggcc ggaccgcgcc tgccgcgcac
     1861 gctcgaccgg cgcgagctgg cgcgcttcgt cctcaccgcg atggaaggcg gcgtgatgca
     1921 ggcgcgcacc catcgcgatc cggcgtactt cgacagctcg gtcaggcagc ttcgcatcca
     1981 tttcgaacat ctggagcgcg aggcggcaca gcagccgtcg cgcgcacgat cgccgcgcaa
     2041 gaaatcaact tcacgaggga gcaaatgatg cgactgaaac ttcgtattac gctggcaggc
     2101 gtgctcgttt ccgccgcggc gttcagcgga tcggccgctg ccgcgccgat cgagccgctg
     2161 caaccgatgg ccttcctcgc cgggcactgc tggaaaggcg atttccccgg cggaaaggag
     2221 accgacgagc attgcttcgc gtggctctac gacggcaagg cgctgcgcga tacgcatacc
     2281 gtgcgcgcac cggggaggcc ggattacatc ggcgagacca cctactactg ggattcggcc
     2341 gccaagcgcg tcgagttcgt ctatatcgag aacctgggcg gcatcagccg cggcaacatg
     2401 caaagcatgc cggaagcgct gatcttccct ccgacgcagt acgtcgccga cggccaggcg
     2461 atgacctacc gcgtgcgctg gacccgattg agcgacacgt cgtacgaagc atggagcgag
     2521 atgcagggca aggacggctg gacgacgatg ttcaagctga cgatgaagcg gtcgtcgtag
     2581 gaatagtcca cgctcgccgt cagccctgaa cggccgcccc cgccgcgagc agctcgcgta
     2641 tttcgtctgc gccgaagccg aattcctcca aaacttcgga cgtgtgttgg ccaagcatcg
     2701 gcgccg
//
LOCUS       DATANS010000046         2192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_170445, whole genome shotgun sequence.
ACCESSION   DATANS010000046 DATANS010000000
VERSION     DATANS010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170445"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VHT22_00665"
     CDS             <1..436
                     /locus_tag="VHT22_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136087.1"
                     /translation="PEGVLVPGGPVARRSSRRKLTLAGAALALVMGGVPFVHAATAQS
                     PPATIETTLRDPWVPPELRTPAPTPAPQGAALRAQVERKLKAGFDAADVNRSGTVTRD
                     QARAAGLGYIVKHFDDIDRQRAGAVSFDDVKRFLRGRGAQLD"
     gene            complement(476..1621)
                     /locus_tag="VHT22_00670"
     CDS             complement(476..1621)
                     /locus_tag="VHT22_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3095 domain-containing protein"
                     /protein_id="HEX3136088.1"
                     /translation="MPDNADFYDRLPVFEGFANIMDPARYRPLPDDWLIGLTDVVDST
                     QAIAAGRYKSVNTAGASVISGIGNALPGRKFPFVFGGDGASFAVPACDRATASAVLAA
                     TAAWARDELDLTLRTALVPVAAVNEQGLTASVARFGPSKHVSYAMFSGGGLAWAERAM
                     KAGRYTLQAAPPGERPDLTGLSCRWNDIPPIHGLIVSLLVMPVTHGDPAFRVVIEALL
                     GELEGSPEVARPVPDNAPDVSWPPPGAELETRTSRRRGESLLLWRLRVLYRTLLSYTV
                     MRTGMRVGEFDPAVYRREVVENSDFRKYDDNLRMTLDCTPALADRIEQRLAKAQRDNI
                     VRFGMHRQPAAIMTCIVPSITESNHVHFIDGAAGGYALAAQRLRAPA"
     gene            complement(1755..>2192)
                     /locus_tag="VHT22_00675"
     CDS             complement(1755..>2192)
                     /locus_tag="VHT22_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX3136089.1"
                     /translation="QGILRIVVAGLFLAHGSAKLLHIPHVAQFDNVQLTSLVGIAGVL
                     ELLGGFLLLIGLFTRPVAFILSGEMAVAYFMAHQPRGLLPILNGGELAAIYSFVFLYF
                     AVAGSGPWSVDERNRARANRMTRRTRADAEFEGRAHDDVEHQL"
BASE COUNT          379 a          762 c          727 g          324 t
ORIGIN      
        1 ccccgagggt gtcctggtgc ccggcggccc tgtcgcccgc cggtcttcgc gccgcaagct
       61 cacgctggcc ggcgccgcgc tggccctcgt gatgggcggg gtcccattcg tgcatgcagc
      121 gacggctcag tcgccgcccg cgacgatcga gacgacccta cgcgatccgt gggtgccgcc
      181 ggagctgcgc acaccggcgc cgacgccggc accgcaaggt gccgccttgc gcgcgcaggt
      241 cgagcgcaag ctcaaggcgg gattcgacgc ggcggatgtc aaccgcagcg gaaccgtgac
      301 gcgcgaccag gcgcgcgccg cgggactcgg ctacatcgtc aagcatttcg acgacatcga
      361 ccgtcagcgc gcgggcgccg tcagtttcga cgacgtcaag cgcttccttc gggggcgagg
      421 cgcccaactc gactgaggcg acagcgcgct ctccgctcga ccacccggcg cgcagtcacg
      481 ccggcgcgcg caatctctgc gcggccagcg cgtatccgcc cgcggcaccg tcgatgaaat
      541 ggacgtggtt gctttcggtg atcgagggca cgatgcaagt catgatcgcg gccggctggc
      601 gatgcatgcc gaagcgcacg atattgtcgc gctgcgcttt ggccaggcgc tgctcgatcc
      661 gatcggcgag cgccggcgtg cagtcgagcg tcatgcgcag attgtcgtcg tatttgcgga
      721 agtccgaatt ctcgacgacc tcgcgccggt agaccgccgg gtcgaattcg ccgacccgca
      781 tgccggtccg catgaccgta tacgacagca gtgttcggta gagcacgcgc agacgccaca
      841 gcagcaggct ctcgccgcgg cggcgcgatg tgcgtgtctc cagctcggcc ccgggcggcg
      901 gccagctgac gtccggcgcg ttgtcgggga ccggtcgagc gacctcggga cttccttcga
      961 gctcgcccag cagcgcttcg atcacgacgc gaaacgcggg atcgccgtgc gttaccggca
     1021 tgacgagcag cgagacgatc aaaccgtgga tcggcggaat gtcattccag cggcacgata
     1081 gcccggtcag atcgggacgc tctccgggcg gcgcagcttg cagggtgtaa cgccccgcct
     1141 tcatcgcgcg ttcggcccag gccagaccac cccccgagaa catcgcatac gacacatgct
     1201 tcgacggccc gaagcgcgcg acgctcgccg tcaagccttg ctcgttgacc gctgccaccg
     1261 gcaccagcgc cgtgcgcagc gtgagatcga gctcgtcgcg cgcccatgcc gcggtcgccg
     1321 cgagaacagc cgaggcggtc gcgcggtcgc acgccggtac ggcgaaactt gcgccgtcgc
     1381 cgccgaaaac aaacgggaac ttgcggcccg gaagcgcgtt gccgatcccg gagatcaccg
     1441 aggcgccggc cgtgttcacc gacttgtaac ggcctgcggc aatcgcctgc gtcgaatcga
     1501 cgacgtcggt gagcccgatc agccagtcat ccggaagcgg ccggtagcgc gccggatcca
     1561 tgatgttggc gaatccttcg aaaaccggca gtcgatcgta gaagtcggcg ttgtccggca
     1621 tggcctgacg cgatctcgcg caaagcacgg gaaagcatca taaccgggac tcgcgttcgc
     1681 cagcgcatcc ggcgcagccg gccgtgcacg gcggaccgcg cgttcgtccg cgcgactatc
     1741 tggcctacgt cgcgctacaa ctgatgctcg acgtcatcgt gcgcgcgccc ctcgaactcg
     1801 gcgtcggcac gcgtccgccg cgtcatccgg ttcgcgcgcg ccctgttgcg ttcgtcgaca
     1861 ctccacgggc cgctgccggc aacggcaaaa tacagaaaca cgaagctgta gatcgcggcg
     1921 agctcgccgc cgttgagtat cggcagcaga ccccggggct ggtgcgccat gaagtaggcg
     1981 acagccattt cgccggacag gatgaatgcg accggacgcg taaagagccc gatcagcagc
     2041 agaaagccgc ccagcagctc tagcacgcca gcgatgccga ccagagacgt cagctgcaca
     2101 ttgtcgaatt gagccacatg cgggatgtgc aacagcttgg ctgaaccgtg cgccaggaac
     2161 agcccggcga cgacgattcg caatatgccc tg
//
LOCUS       DATANS010000047         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_506850, whole genome shotgun sequence.
ACCESSION   DATANS010000047 DATANS010000000
VERSION     DATANS010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506850"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1489)
                     /locus_tag="VHT22_00680"
     CDS             complement(<1..1489)
                     /locus_tag="VHT22_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136090.1"
                     /translation="MAAALLRGRAAAGVWTARVQRALPALGLAGLAAIGLSGRLQGDT
                     PPLLIDRAQAEATADAAVAQRGMKAGDGWRRFSTIRLVSDDAGAAQWHQFVWREAGRD
                     AYEKLIGNWLAPPLWEVRYARFDGGDVADRAEEWRVTVAGDGTLRQVRHPLPEKRPGA
                     TLSREQARSLAQQEIRQRFGLDPATLREVSAEEQARPARADWQFTYADPAVEVGKGGE
                     ARVITAVSGDEIVGSGRYVFVPETWQRAERLRASRLSLAKNAITLALIVAGLAAIIAT
                     IIAWSRGRFDKRAFWLALGTVGAASLVTGINAWPAFAMQLKTAEPLAPQIALGAAGIA
                     LSLVPLTLLASLLAGVAVWEAREHSAPDTGVGRLWLRGASAGFFVAGVEGLAGAVAAQ
                     DFPRSARYTVENAWLPWVDALVRSVSAPVLGVAVTIIALHWLERWTDGWRRRRWLVYA
                     ALTIATAALAALRADNWTAIIVGGLVGGALYTLLFATVLRYDLRLV"
BASE COUNT          242 a          574 c          504 g          211 t
ORIGIN      
        1 gcaccagtcg aagatcgtag cgcagcacag tcgcgaacag cagcgtgtag agcgcgccac
       61 cgaccagtcc gccgacgatg atggccgtcc aattatcggc cctcaacgcc gccagcgcgg
      121 cggtcgcgat cgtcaatgcc gcgtagacca gccagcggcg gcggcgccaa ccgtcggtcc
      181 agcgctccag ccagtgcagc gcgatgatcg tcaccgcgac accgaggacc ggcgcggaaa
      241 ccgatctgac cagcgcgtcg acccacggga gccatgcgtt ctccaccgtg tagcgcgccg
      301 atcggggaaa gtcctgcgcg gccaccgcgc cggccagacc ttcgacgccg gcgacgaaga
      361 accccgcgct ggccccgcgc agccataggc gaccgacgcc cgtgtcgggc gccgaatgct
      421 cgcgagcttc ccagacggcg acgcccgcca gcaggctcgc gagcaaagtg agcggaacga
      481 gcgacagcgc gatccccgct gcgccgagcg cgatttgcgg cgccagcggc tcggcggtct
      541 tgagctgcat cgcgaacgcg ggccaggcgt tgatcccggt cactagcgac gccgcgccga
      601 ccgtacccaa tgccagccag aacgcgcgct tgtcgaaacg cccccggctc caggcgatga
      661 tcgtcgcgat gatcgctgcg agtccggcga cgatcaatgc cagcgtgatc gcgttttttg
      721 caaggctcaa tcggctcgcc cgcaggcgtt cggcgcgttg ccatgtctcg ggaacgaaaa
      781 catagcgacc ggatccgacg atctcgtcgc cggagactgc cgtgatcacg cgcgcctcgc
      841 cgcccttgcc gacctcgacg gccggatcgg cgtaggtgaa ttgccaatcg gctcgcgcgg
      901 gtcgcgcttg ctcctcggcc gacacctcgc gcagtgtcgc cggatcgagt ccgaagcgtt
      961 gccggatctc ctgctgggcg agcgagcgcg cctgttcgcg cgagagcgtc gcacccggcc
     1021 gcttctctgg cagcgggtgg cgcacctgcc gcaacgttcc gtcgccggcc acggtgacac
     1081 gccactcctc ggcgcggtcg gcgacatcgc cgccgtcgaa gcgcgcatag cgcacttccc
     1141 acagcggcgg cgcaagccag ttgccgatca gtttttcata cgcatcgcgc cccgcttcgc
     1201 gccagacgaa ctggtgccat tgcgccgccc ccgcgtcatc gctgacgagg cggatggtcg
     1261 aaaagcgccg ccacccgtcg ccggctttca tcccgcgttg cgcgacggct gcgtcggcgg
     1321 tggcctcggc ctgggcccga tcgatcagca gcggcggcgt atctccctgc agccggccac
     1381 tcaagccgat cgctgccaac ccggcaaggc cgagcgccgg caacgcgcgc tgtacgcgcg
     1441 cggtccatac gcctgccgcg gcgcggccgc gcagcagcgc ggcggccacc ggcgcagccg
     1501 gtgcttgcca agcgccgttg cgcattgcct c
//
LOCUS       DATANS010000048         2108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_338927, whole genome shotgun sequence.
ACCESSION   DATANS010000048 DATANS010000000
VERSION     DATANS010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338927"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /gene="hprK"
                     /locus_tag="VHT22_00685"
     CDS             <1..864
                     /gene="hprK"
                     /locus_tag="VHT22_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEX3136091.1"
                     /translation="GGNRVLTGEAALKPTIGQVGHMNFIHPFRVQMIGAAEASYLRSL
                     PEDALQQAISRLFTTDLAAIIVANGEEIPKYLLDACNRHHMALLTSPQPSPHVVDVIR
                     LYLSRMLAESTSLHGVFLDVLEMGVLITGASAIGKSELALELISRGNGLVADDIVELY
                     RISPDTLEGRCPAVLKDFLEVRGIGVLNIRTIFGETAVRPRKLLKLMVHLEDHSNEQF
                     SELDRLQVSATYQEILGVPIRKVTIPVAAGRNLAVLVEAAVRNFVLNQRGIDSTKEFI
                     ERQQKLMEDGG"
     gene            874..1710
                     /gene="rapZ"
                     /locus_tag="VHT22_00690"
     CDS             874..1710
                     /gene="rapZ"
                     /locus_tag="VHT22_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX3136092.1"
                     /translation="MDLVLIGGVSGSGKSVALAALEDSGYYAVSNLPSTQLGGLLEHL
                     KRAQQERVAIALDVKTDPALAALQAAIEAARADGWSVRFVYLDAKTETLVKRFSETRR
                     RHPFSSDDRTLREAIEFERARLADIRSLGYSFDTSDLPAAALRRWVKDFISVDPSRMT
                     LLFQSFGYKHGVPLDADLVFDVRCLPNPHYEPALAPLTGKDAAVIAYLESHPEVERMY
                     TDIYHFVASWLPDYARDNRNYLTVGIGCTGGRHRSVYLAERLAQAFGGRYQVHTRHRE
                     IG"
     gene            1791..>2108
                     /locus_tag="VHT22_00695"
     CDS             1791..>2108
                     /locus_tag="VHT22_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEX3136093.1"
                     /translation="MTKAKTVTVAFTGASGLPYGLRLLECLIAARARVFLLYSPAAQV
                     VARQELELTLPTQPAAAAAYFAERYRAEPGQLTVFGREDWMSPVASGSNPPDAMAVCP
                     CSMG"
BASE COUNT          355 a          708 c          685 g          360 t
ORIGIN      
        1 ggcggcaacc gcgtgctgac cggcgaggca gcgctgaagc cgacgatcgg ccaggtcggg
       61 cacatgaatt tcatccaccc gttccgcgtg cagatgatcg gcgccgccga ggcgagctat
      121 ctgcgcagcc tgcccgagga cgcgctgcag caggcgattt cgcgcctgtt caccaccgac
      181 ctcgcggcga tcatcgtcgc caacggcgaa gagattccca aatatctgct cgacgcatgc
      241 aaccggcatc acatggcgct gctgacctcg ccgcagccgt cgccgcacgt cgtcgatgtg
      301 atccgcctct atctgtcgcg catgctcgcc gaatcgacgt cgctgcatgg cgtgtttctc
      361 gacgtgctcg aaatgggcgt gctgatcacc ggcgcatccg cgatcggcaa gagcgagctc
      421 gcgctcgagc tcatctcgcg cggcaacggg ctggtcgccg acgacatcgt cgagctgtac
      481 cgcatttcgc cggacacgct cgagggacgt tgcccggccg tgctgaagga ctttctcgaa
      541 gtgcgcggca tcggcgtgct caacatccgc acgattttcg gcgagaccgc cgtgcgcccc
      601 cgcaagctcc tcaagctgat ggtccacctc gaggatcaca gcaacgagca attctccgag
      661 ctcgaccggc tgcaggtcag cgcgacctat caggagatcc tgggcgtgcc gatccgcaag
      721 gtgacgattc cggtggccgc cggtcgcaat ctggcggtgc tggtcgaggc ggcagtgcgc
      781 aatttcgttc tcaaccagcg gggcatcgac agcaccaagg agttcatcga gcgccagcag
      841 aagctgatgg aagacggcgg ctgacgcgcc acgatggacc tcgtcctgat cggcggcgtc
      901 tcgggctcgg gcaagagcgt cgcgcttgcg gcgctggaag acagcggcta ttacgcggta
      961 tcgaatctgc cgtcgacgca gctcggcggc ctgctcgagc atttgaagcg ggcgcagcag
     1021 gagcgcgtgg caatcgcgct cgacgtcaag accgatcccg ccctcgccgc cttgcaggct
     1081 gcgatcgaag ccgcgcgcgc cgacgggtgg agcgtgcgct tcgtctatct ggacgccaag
     1141 accgagacgc tggtcaagcg cttttcggaa acccgccgca ggcatccctt ttccagcgac
     1201 gaccgtacgc tgcgcgaggc gatcgagttc gagcgcgcgc gtctggccga catccgctcg
     1261 ctgggctaca gcttcgacac aagcgacctg ccggcggcgg cgctgcgccg ctgggtcaag
     1321 gacttcatca gcgtcgatcc atcgcggatg acgctgctgt tccagtcgtt cggatacaag
     1381 cacggcgttc cgctcgacgc ggacctagtc ttcgacgtcc gctgcctgcc gaatccgcac
     1441 tacgagccgg cgctcgcgcc gctcaccggc aaagacgcgg ccgtcatcgc ctaccttgag
     1501 agccatcccg aggtcgagcg catgtacacc gatatatacc atttcgtcgc gagctggctg
     1561 cccgattacg cgcgcgacaa tcgcaactat ctgacggtgg ggatcggctg caccggcggc
     1621 cggcatcgct cggtctattt ggctgagcgg ctggcgcagg cgttcggcgg ccgctatcag
     1681 gtgcacacgc gccaccgcga gatcggctga gtatcgggca gagcatcgga cgcgttccgc
     1741 ggcgtcggcc gcctcggccg acagtgtgcg caataagcta gaatcacccg atgactaaag
     1801 cgaagaccgt caccgtcgca ttcaccggcg cgtcgggatt accgtatggc ttgcgcctgc
     1861 tcgaatgcct gatcgcggcg cgggcgcgcg tcttcctgct gtactccccg gcggcgcagg
     1921 tcgtcgccag acaggagctc gagctcacgc ttcccacaca gcctgccgcc gcggcggcct
     1981 atttcgccga gcgctatcgc gcagagccgg gtcagctgac ggtgttcggc cgggaggact
     2041 ggatgagtcc ggtggcgtcg ggatcgaacc cgcccgatgc gatggctgtc tgcccgtgca
     2101 gcatggga
//
LOCUS       DATANS010000049         1860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_2740, whole genome shotgun sequence.
ACCESSION   DATANS010000049 DATANS010000000
VERSION     DATANS010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2740"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="VHT22_00700"
     CDS             complement(<1..1125)
                     /locus_tag="VHT22_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136094.1"
                     /translation="MTVSHARPPRLPLPTIVSRAAMRTHAMPRGPLWRGARYTHEDRA
                     RALRRGLRFIYRLACVPKNFEANGEDFLWCFYSVSAATADPWLRAAAWRMGQERARYW
                     RRQNRRPPRSPDADDVMSWAFGDYAADCLNVRDERIKPLVARAARKFRPVKFLYFDPT
                     KEPIPRDMPDECEHCGASNARGRRHCRACGRKLRMFNRYEVMIDALVTSYSGEAYGVP
                     LGGRFHDVTALLPMLRPYRGYEGGAHRGYIDLVYAVTHVVYTLNDYGLYRLRPAWLPD
                     EFAFLKANLRQSIADRDPETTGEFLDTLKAFGLTEADPVIRAGMDFVLSRQRSDGSWA
                     EEEPDDCYTAYHATWTAIGGLMDHAYRGERTRYPEALRRAQ"
     gene            complement(1213..1443)
                     /locus_tag="VHT22_00705"
     CDS             complement(1213..1443)
                     /locus_tag="VHT22_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136095.1"
                     /translation="MGETAFRGSIESARKWHALAERRRRHYVELYRSERWRRYFKEET
                     FLLLMRDAIQNAENWAKILERMAGAGGSAKAA"
     gene            complement(1708..>1860)
                     /locus_tag="VHT22_00710"
     CDS             complement(1708..>1860)
                     /locus_tag="VHT22_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX3136096.1"
                     /translation="GAFVIIAMQNYLNALDQWVTVIQGVIFVACVLLFRRGIIGEIAN
                     LLRIKL"
BASE COUNT          350 a          611 c          596 g          303 t
ORIGIN      
        1 ctgcgcccgc cgcaacgcct ccgggtagcg tgtgcgctcc ccgcggtagg cgtggtccat
       61 caggccaccg atcgccgtcc aggtcgcatg ataggcggta tagcaatcgt ccggctcctc
      121 ttcggcccag cttccgtcag aacgctggcg tgacagcacg aaatccatgc cggcgcggat
      181 gaccggatcg gcctcggtca ggccgaacgc cttcagcgtg tcgaggaact caccggtggt
      241 ttcagggtcg cggtcggcga tgctctgccg caggttcgcc ttgaggaatg cgaactcatc
      301 cggcaaccag gccgggcgca ggcgataaag accatagtcg ttgagcgtgt agacgacgtg
      361 cgtcaccgcg tagacgagat cgatgtagcc ccgatgtgcg ccgccctcgt accctcgata
      421 gggtcgcagc atgggcaaca gagccgtgac atcgtggaac cgaccgccca gcggcacacc
      481 ataggcttcg ccactatagc tcgtgaccag cgcgtcgatc atcacctcgt agcgattgaa
      541 catccgcagc ttgcggccgc aggcgcggca atgcctgcgt ccgcgcgcgt tggaggcccc
      601 gcaatgctcg cactcgtcgg gcatatcgcg cggaatcggc tccttggtcg gatcgaagta
      661 gaggaacttc accgggcgga attttcgcgc cgcgcgcgca acgagaggct tgatcctctc
      721 atcgcgcacg ttgaggcagt cggcggcgta atccccgaag gcccaggaca tgacatcatc
      781 ggcgtcggga gatcgcggcg gccggcgatt ttgccgccgc caatagcgcg cccgctcctg
      841 ccccatgcgc caggccgcgg cgcgcagcca cggatccgcc gtggcggctg agaccgaata
      901 gaagcaccat aggaagtctt cgccattcgc ctcgaagttc ttcggcacgc aggcgaggcg
      961 atagatgaag cgcagtccgc gccgcagcgc acgggcacgg tcctcatgcg tgtagcgcgc
     1021 gccgcgccag agcggcccgc gcggcatcgc gtgcgttcgc atcgccgcgc gcgacacgat
     1081 ggtcgggagc ggcagccggg gcggtcgggc gtgagagacg gtcacgcggc cattgcatac
     1141 cgctgcgcgg ccaaacgcaa gctacgcgcg catagacaca cgcatttccg ctatcgttgt
     1201 tgaaagcttt cctcaggcgg ctttcgcgct gcccccggca cccgccatgc gctcgaggat
     1261 tttcgcccag ttttccgcgt tctggatggc gtcgcgcatc agcagcagga aggtttcttc
     1321 cttgaaatag cggcgccagc gttcgctgcg gtagagctcg acgtaatggc ggcggcggcg
     1381 ttcggcgagc gcatgccact tgcgcgccga ttcgatcgag ccacgaaacg ctgtctcacc
     1441 catgctccgg cctcaaaagt gcgcaaaacc gtgagcgccg ggcacacgca cccaacgcac
     1501 cgcaacaaac gccacgccac aacagacttg ggagaggctg aactcacaca gccgacggat
     1561 tgcccctacg gcgcaaagtg tcccgcggga gcgcctggca ggcaataact tacgggaagt
     1621 ggagcggcgc catgtggaca acggcgcgat tatttcatcg accgttgccg atcggcatcg
     1681 gttccggcag cccgcgactg atgcaggtta gagcttgatg cggagcaggt tcgcgatctc
     1741 accgatgatg ccgcggcgga acaagagcac gcaggcgacg aagatcacgc cctggataac
     1801 cgtcacccac tggtcgagcg cattgaggta attctgcatc gcgatgatga cgaacgcgcc
//
LOCUS       DATANS010000050         1568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_296269, whole genome shotgun sequence.
ACCESSION   DATANS010000050 DATANS010000000
VERSION     DATANS010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296269"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..179
                     /locus_tag="VHT22_00715"
     CDS             15..179
                     /locus_tag="VHT22_00715"
                     /inference="COORDINATES: protein motif:HMM:NF015881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal L9 C-terminal domain-containing
                     protein"
                     /protein_id="HEX3136097.1"
                     /translation="MGGLGHTVEKAMVRMPDGPLKHVGEFPLTIALHADAVAHINVHV
                     LGDTTGVQAG"
     gene            343..>1568
                     /gene="dnaB"
                     /locus_tag="VHT22_00720"
     CDS             343..>1568
                     /gene="dnaB"
                     /locus_tag="VHT22_00720"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX3136098.1"
                     /translation="MPNDRELEALKVPPHSIEAEQSVLGGLLLDNGAADRVADILGTE
                     DFYSDAHRLLYKTTVGLIADNKPADVVTLAEALQSLTKLDYIGGMAYLGALVENVPTA
                     ANIRRYAEIVRERAILRRLAAAGGEIADAAYHPLGRSVREILDHAETKVFEIAEHGAR
                     AQQGFQEIRPLLTQVVERIEFLYNRDNPSDVTGVATGFTDLDRMTSGLQEGDLVVIAG
                     RPSMGKTALALNIGEHIALTSKMPVAIFSMEMGATQLAMRLMGSVGKLDQQKIRTGRL
                     SNDDWERLSGALGRLNDAPIHIDDTAALNPLELRARARRLSRQYGGKLGAIVVDYLQL
                     MQAVGEGENRATEISEISRSLKALAKELKVPVMALSQLNRSLESRTDKRPVMSDLRES
                     GAIEQDADVILFIYRD"
BASE COUNT          289 a          516 c          507 g          256 t
ORIGIN      
        1 acatcgtcga cgcgctggga ggccttggcc acacggtaga gaaggcgatg gtgcgcatgc
       61 ccgacggtcc gctgaagcat gtcggcgagt ttccgctgac gatcgcgctg cacgccgacg
      121 ccgtggcgca catcaatgtc cacgttctgg gcgatacgac cggggtccag gcgggctgac
      181 ctgccgttcg cgctctcact cgatctgggg ccggaaacgg ccccatttct ttggcggcgc
      241 gcgtaaactc ccggctgatc attcagcagg aatagccaac cgcaaagcgc ggcgcgcaac
      301 tgctagaatg cctgtgcctt ccgcaagtcc gaatccttcc gcatgcccaa cgatcgcgag
      361 ctcgaagcgc tcaaggttcc gccccattcg attgaggccg aacagtcggt tttgggcggg
      421 ctcctgctcg acaacggcgc cgcggatcga gtggccgaca tcctcggcac cgaggatttc
      481 tacagcgatg cgcatcgcct gctgtacaag acgacggtcg ggctgatcgc cgacaacaag
      541 ccggccgatg tcgtcacctt ggccgaggcg ctgcagtcgt tgaccaagct cgactatatc
      601 ggcggcatgg cctacctcgg cgcgctggtc gagaacgtgc cgacggccgc caacatccgg
      661 cgctatgccg agatcgtgcg cgagcgcgct atcctgcgcc ggctcgctgc cgccggcggc
      721 gagatcgccg acgcggccta ccatccgctg ggccgcagcg tgcgcgaaat cctcgatcac
      781 gccgagacca aggtattcga aatcgccgag catggtgcgc gcgcccagca gggtttccag
      841 gagatccgtc cgctgctgac ccaggtcgtc gagcgcatcg agttcctata caaccgcgac
      901 aatccctccg acgtgactgg cgtcgcgacc gggttcaccg acctcgaccg catgacttca
      961 ggccttcagg aaggcgacct ggtcgtcatc gccggtcggc cgagcatggg gaaaactgcc
     1021 ctggcgctca acatcggcga gcacatcgcg ctgacatcga agatgccggt cgcaattttc
     1081 agcatggaaa tgggcgccac gcagttggcg atgcgcctga tgggctcggt cggcaagctg
     1141 gaccagcaga aaatccggac cgggcgcctt tcgaacgacg actgggagcg gctttccggc
     1201 gcgctggggc gcctgaacga cgcgccgatc cacatcgacg acaccgcggc gctgaatcct
     1261 ctcgagttgc gggcgcgggc gcggcgcctg tcgcgccagt acggcggcaa gctcggtgcg
     1321 atcgtcgtcg actatctgca gctgatgcag gcggttggcg aaggcgagaa ccgcgcgacc
     1381 gagatctccg agatatcgcg ctcgctgaag gccctggcaa aggagctcaa ggtcccggtg
     1441 atggcgctat cgcagctcaa ccgaagcctc gagtcccgaa ccgacaagcg gccggtgatg
     1501 tcggacctgc gcgaatcggg agcgatcgag caggacgccg acgtgatcct gttcatctac
     1561 cgcgacga
//
LOCUS       DATANS010000051         2278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_674257, whole genome shotgun sequence.
ACCESSION   DATANS010000051 DATANS010000000
VERSION     DATANS010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674257"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VHT22_00725"
     CDS             <1..339
                     /locus_tag="VHT22_00725"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX3136099.1"
                     /translation="HVKSGKLRAIGLTSLKRSPALPDLPTMHELGIANFQSIGWYGVL
                     VPTGTPKPLVARLNRELVAALGQPDLRERLASQGGDAVSSTPEQFAAFIADEMKKWTN
                     VIRTANIQVE"
     gene            399..1802
                     /locus_tag="VHT22_00730"
     CDS             399..1802
                     /locus_tag="VHT22_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX3136100.1"
                     /translation="MSAHESSNENRYTRGIAEFVSTLRYEDIPANVLTRIKLLMLDSL
                     GCALYGADLEWTRIMQRRLGELDTTQTCAVWGTRQKISAPHAALINGTQVQGFELDDV
                     HRQGVLHVGAVVLPALISITEMRPGMSGKEFLASAVAGYEIGPRVGICMGPEHIAQGW
                     HSGATLGVFSAAAGAARGLKLDVDKTVHALGIAGTQAAGLMAAQYGAMVKRMHAGRSS
                     QSGLYGALLADAGFTGIVNVLESEYGGFCTTFSRSTDRFKLDELIKDFGKVWQTMGVA
                     LKFYSCVGSNHTTLDAIRLMQQERPFGADDVKSIVVHGSQVTMDHVGWKYVPQGLTSA
                     QLNLPYCVATWLLDGDCFVNQFTEDKVADPLRMKHAEKVSVKHDDAITAKGSKFRHMV
                     RVEVFLNDGKRMERTVEAGRGNENNFASEAEVCEKFEKLAAAALPKPQIEKLRDAMLK
                     LETLKDATEIARLLARA"
     gene            1816..>2278
                     /locus_tag="VHT22_00735"
     CDS             1816..>2278
                     /locus_tag="VHT22_00735"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX3136101.1"
                     /translation="MVKRLSWLFAATLCAAQVQAQEYPTRPIRLIVPLAPAGGMDTIA
                     RGIAQKLTESLGQSVVVDNRSGGGGVIGVETVAHSTPDGYTLLMMSATALIYPLMYKA
                     KYDIARDFAPIVQVTTQPYVIVVNPNVPVKSVAELIAYAKANPNKLNFASSG"
BASE COUNT          464 a          742 c          702 g          370 t
ORIGIN      
        1 cacgtgaaaa gcggcaagct gcgtgcgatc ggactcacga gcctcaagcg ttcgccggcg
       61 ctgcccgacc tgccgacgat gcacgagctt ggcatcgcga attttcagtc gatcggctgg
      121 tacggcgtgc tcgtcccgac cggcacgccg aagccgctgg tcgcgcgcct gaaccgcgag
      181 ctcgtcgccg cgctcggcca gccggacttg cgagaacgac tcgccagcca gggcggcgac
      241 gccgtatcgt cgacgccgga acaattcgcc gctttcatcg cggacgagat gaagaaatgg
      301 acgaacgtca tccgcacggc caacatccag gtcgagtgac cgaaaacgca ctaaaatcga
      361 cgtttctttt tcaacatttt tcgcgggagc actaaccgat gtccgctcat gaatccagca
      421 acgaaaaccg ttacacccgc ggcatcgccg aattcgtgtc gacactgcgc tacgaagaca
      481 ttcccgccaa tgtgctcacg cgcatcaagc tgttgatgct cgactcgctc ggctgcgcgc
      541 tctacggtgc cgatctcgaa tggacgcgca tcatgcagcg gcggctcggc gaactcgaca
      601 ccacgcagac gtgcgcggtg tggggcacca ggcaaaaaat ctcggcgccg cacgcggccc
      661 tcatcaacgg cacgcaggtg cagggtttcg agctcgacga cgtgcatcgc cagggcgtgc
      721 tgcacgtggg cgcggtggtg ctgcccgcgc tgatctcgat caccgaaatg cgcccgggca
      781 tgagcggcaa ggaattcctc gcgtccgcag tcgcgggtta tgagatcggc ccgcgcgtcg
      841 gcatctgcat gggccccgag cacatcgcgc agggctggca ttcgggcgcg acactcggcg
      901 tgttctccgc cgcagcgggc gctgcgcgcg gattgaaact cgatgtcgac aagaccgtgc
      961 acgcgctggg catcgccggc acgcaagccg cgggcttgat ggccgcgcaa tacggcgcga
     1021 tggtcaagcg catgcacgcc ggccgctcgt cgcaaagcgg tctctatggc gcgctgctcg
     1081 ccgacgcggg cttcaccggc atcgtcaacg tgctcgagag cgaatacggc gggttctgca
     1141 ccacgttttc gcgctcgacc gaccgcttca agctcgacga actcatcaag gacttcggca
     1201 aggtctggca gacgatgggc gtcgcgctca agttttattc gtgcgtcggc agcaaccaca
     1261 cgacgctcga cgcgattcgg ctcatgcagc aggaacgccc gttcggcgcc gacgacgtaa
     1321 agagcatcgt cgtgcacggc tcgcaggtca cgatggacca cgtcggctgg aaatacgtgc
     1381 cgcaggggct cacgtccgcg cagttgaacc tgccttactg cgtcgcgacg tggctgctcg
     1441 acggcgattg cttcgtcaac cagttcaccg aggacaaggt cgccgacccg ctgcgcatga
     1501 agcacgccga gaaggtgtcg gttaagcacg acgacgcaat caccgcgaaa ggctccaagt
     1561 tccgccacat ggtgcgtgtc gaagtgttcc tgaacgacgg caagcgcatg gagcgcaccg
     1621 tcgaagccgg ccgcggcaat gaaaacaatt tcgcatccga agccgaggtg tgcgaaaaat
     1681 tcgagaagct cgcggcagcc gcgctgccca agccgcagat cgagaagctg cgcgatgcga
     1741 tgctgaagct cgaaacgctc aaggatgcca ctgagattgc gcgactgcta gccagagctt
     1801 aacgcccgac aaccaatggt gaagagatta tcctggctgt tcgccgcgac actgtgcgcg
     1861 gcgcaagttc aagcacaaga gtatccaacc cggccaatac gcctgatcgt cccgctcgca
     1921 cccgccggcg gcatggacac catcgcgcgc ggcatcgcgc agaagctgac cgagagcctc
     1981 ggccagtccg tggtggtgga caaccgcagc ggcggcggcg gcgtgatcgg cgtcgagacc
     2041 gtcgcgcatt cgacgcccga cggctatacg ctgctgatga tgagcgcaac ggcgctgatt
     2101 tatccgctca tgtacaaggc aaaatacgac atcgcgcgcg actttgcgcc gatcgtgcag
     2161 gtcaccacgc agccgtacgt gatcgtggtg aatcccaacg tcccggtcaa atccgtcgcg
     2221 gagctgatcg cctacgcgaa agcgaacccc aacaagctca acttcgcctc atccggca
//
LOCUS       DATANS010000052         2927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_170860, whole genome shotgun sequence.
ACCESSION   DATANS010000052 DATANS010000000
VERSION     DATANS010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170860"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /locus_tag="VHT22_00740"
     CDS             <1..1462
                     /locus_tag="VHT22_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3136102.1"
                     /translation="TAAAYEAFGIHQWTARLWPALAGFAGVLFIGYVGFRLGGPRLGL
                     YSAAALGGCLWYVLNTHILTLDSGLTLWMSVGFGALLLAQRSLALEREQRMWMLIAWS
                     ALALAVLSKGLIGVVLPGASLFVYSLLTRDWAVWRRLHLLGGLLLFALIAAPWFIAVS
                     LANPEFFRFFFIHEHFERFLTNEHHREGAWWYFIPILAAGILPWLTVLAWTAPRIWSG
                     PVVDSNGFSWQRFALVWSAFIVVFFSVSSSKLPSYILPIFPALALVIGVQLAALPERT
                     LIWLTLPLVVATGTCMLAIGFGFDAIAAHFADERQPLAPLLAYGPWLAAGSAVALAGG
                     VAALWWLKGGKRTAAILAVSLTTLLAALLVLTGHDELADSRSAAPILSRVVSQHGPIA
                     ADVPFYSVKMYDQTLPWYLGRTVTPVAHLDELAMGLATEPDKAVATVDEWKQRWDRET
                     QAYAIMQVDMYEMLLREGVPMRELGRDPRRVIVSRR"
     gene            1528..1899
                     /locus_tag="VHT22_00745"
     CDS             1528..1899
                     /locus_tag="VHT22_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HEX3136103.1"
                     /translation="MNLSGFSFLITGVLLNAIAQLLLKAGTNTLGVITLTRTNWTGEF
                     GRMATEPHFIGGVICYGVSLIVWILGLSRVPVSIAYPMLSVGYVINAIAAYYLFGEGV
                     SFMRWLGIGFIVLGVWLVARS"
     gene            1912..>2927
                     /locus_tag="VHT22_00750"
     CDS             1912..>2927
                     /locus_tag="VHT22_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEX3136104.1"
                     /translation="MSARAQAAQAAYLKFAGPDLDEATIAGVAEVLRSGQITSGPWVT
                     RFERQLEEFCAGRPVRVLTSATAAIEVALQLCGVGPGDEVITGAQSFFTVLNMIVKVG
                     ATPVFVDCDLVTRNIDLAQVESAITPRTKAIIPTHFPGALVDLDAVYALAKRRGLRVI
                     EDAALVLGSRWREQNVGAVGDIVTFSFHPNKNATSIEGGALVVNDKDEARRVETLRFH
                     GISHLPDRTRDVGFPGGKFNLPDVNARIGHAQIERLPEFLAKRRALVDRYFTRFATEP
                     ACVLPPRPAGADDGHSWNMFCVLLPLDSMTIDRNGFRDAMEARGIGTGVSYEALHLCT
                     LGRR"
BASE COUNT          431 a          956 c          965 g          575 t
ORIGIN      
        1 gaccgccgct gcatacgagg cgttcggcat ccatcaatgg acagcgcgtc tttggcccgc
       61 gctcgccgga ttcgccggcg tgctgttcat cggttacgtc ggatttcgcc tcggcggtcc
      121 ccgtctgggg ctatacagcg cggcggcgct tggcggatgc ctatggtatg tgctcaacac
      181 gcacatcctc accctggact cgggactcac cttgtggatg agcgtcggct tcggcgcgct
      241 gctgctggcg caacgaagct tggcgctgga gcgcgagcaa cgcatgtgga tgctcatcgc
      301 ctggtcggcg ctggcgctgg ccgtgctgtc gaagggactg atcggcgtcg tcctgcccgg
      361 cgcctcgctt ttcgtatatt cgctgctgac ccgcgactgg gccgtgtggc ggcggctgca
      421 tctgcttggc ggcctgctgc tgttcgcgct gatcgccgcg ccatggttca tcgccgtgtc
      481 gctggccaat ccggaattct tccgtttctt tttcatccac gagcatttcg aacgcttcct
      541 caccaacgaa catcatcgcg aaggcgcgtg gtggtatttc attccgatcc tcgccgccgg
      601 cattctgccg tggctgaccg tcctcgcctg gacggcgccg cggatatggt cgggcccggt
      661 cgtcgacagc aatggcttca gctggcagcg cttcgcgctc gtctggtcgg ccttcatcgt
      721 tgtttttttc agcgtgtcga gctccaagct gccttcgtac atattgccga tctttccggc
      781 gctggcactg gtgatcggcg tacagctggc ggcgctgccc gagcgcacgc tgatctggtt
      841 gaccctgccg ctggtcgtcg ccaccggcac gtgcatgctg gcgatcggct tcggcttcga
      901 cgccatcgcg gctcacttcg ccgacgagcg ccagcccctg gctccgctgc tggcctacgg
      961 cccgtggctc gctgccgggt ctgcagttgc gctggccggc ggcgtggccg cgctctggtg
     1021 gctcaaaggc ggcaagcgca cggcggcgat attggcggtg tcgttgacca cgctgctggc
     1081 tgcgctgctg gtgctgaccg gccatgacga gctcgccgac tcgcgctcgg ccgcaccgat
     1141 cctctcccgc gtcgtcagcc agcacgggcc gatcgccgcc gacgtgccgt tctattcggt
     1201 caagatgtac gatcagacgc tgccgtggta tctgggccgc acggtgacgc cggtcgcgca
     1261 tctggacgaa ctggcgatgg ggctggccac cgagcccgac aaggcggtgg cgacggtgga
     1321 cgagtggaag cagcgctggg accgggaaac ccaagcgtat gcgatcatgc aggtcgacat
     1381 gtacgagatg ctgctgcgcg agggcgtgcc gatgcgggag ctcggccgag acccgcgccg
     1441 cgtgatcgtc agccgccgtt gacggccgac accagaaatc gacctaaagg ggcgagcgcc
     1501 gatgcattcc agcgataccg gccggcaatg aacctgtcgg gattttcgtt tctgattacc
     1561 ggcgtgctgt tgaacgcaat cgcgcagctg ctgctcaagg caggcaccaa tacgctgggc
     1621 gtcatcacgc tgacccgcac caactggacc ggcgaattcg gcaggatggc gaccgagccg
     1681 catttcatcg gcggggtgat ctgttacggg gtcagcctga tcgtgtggat actcgggctc
     1741 tcgcgcgtgc cggtgtcgat cgcctatccg atgctgtcgg tgggctatgt gatcaacgcg
     1801 atcgctgcct attatctgtt cggcgagggc gtgagcttca tgcgctggct cggcatcggc
     1861 ttcatcgtgc tcggcgtgtg gctggtggcg aggagctgac gggatgggcc aatgagcgcg
     1921 cgcgcgcagg cggcgcaggc tgcctatctc aaattcgccg gtcccgatct ggacgaagcg
     1981 acgatcgccg gcgtcgccga ggtcctgcgc tcaggccaga tcaccagcgg gccctgggtc
     2041 actcgattcg agcggcagct cgaagaattc tgcgccggcc ggccggttcg cgtgctgacc
     2101 tctgcaaccg ctgcgatcga ggtcgcgctg cagctttgcg gcgtcggccc cggcgacgaa
     2161 gtgatcaccg gtgcgcaaag ctttttcacc gtgctcaaca tgatcgtcaa agtgggcgcg
     2221 accccggtgt tcgtcgactg cgacctggtc acgcgcaaca tcgaccttgc gcaagtcgaa
     2281 tctgcgatca cgccgcggac caaagcgatc atcccgactc attttcccgg cgcgctggtc
     2341 gacctggacg ccgtttacgc gttggccaag aggcgcggcc ttcgcgtcat cgaggacgca
     2401 gcgctcgtgc tgggatcgcg ctggcgcgaa cagaacgtcg gtgccgtcgg cgacatcgtc
     2461 accttcagct ttcatccgaa caagaacgcg acatcgatcg aaggcggcgc cctggtcgtc
     2521 aacgacaagg acgaagcgcg gcgcgtcgaa acgcttcgct tccacggcat cagccacctg
     2581 cccgaccgca cgcgcgacgt cgggtttccc ggcggcaagt tcaacctgcc ggatgtcaac
     2641 gcccgcatcg gtcatgcgca gatcgagcgt ctgccggagt tcctggccaa gcgccgcgcg
     2701 ctcgtcgacc gctatttcac ccgctttgcg accgagccgg cgtgcgtgct gccgccgcgg
     2761 ccggcgggcg ccgacgacgg ccattcctgg aacatgtttt gcgtcctgct cccgctggat
     2821 agcatgacga tcgatcgcaa cggtttccgc gacgcgatgg aggcgcgcgg catcgggacc
     2881 ggcgtatcgt acgaagcgct gcatctttgc acgctcggcc ggcgttt
//
LOCUS       DATANS010000053         2192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_464820, whole genome shotgun sequence.
ACCESSION   DATANS010000053 DATANS010000000
VERSION     DATANS010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464820"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VHT22_00755"
     CDS             <1..323
                     /locus_tag="VHT22_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX3136105.1"
                     /translation="SNDKLHTFKAKATYYHDRKYGGTLAYFSTTGSADSGLYGTNADG
                     NAIKPDSNGYIFELDYLPIQNVRLMFQYTAYSKFDGASTNYDGNGRNARDNNTAFFNI
                     WVAF"
     gene            424..1014
                     /locus_tag="VHT22_00760"
     CDS             424..1014
                     /locus_tag="VHT22_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX3136106.1"
                     /translation="MLFAGAVASAGAQNNPADVQKAAEEKAVNLCSTCHGPRGISTSP
                     EFPILAAQRKGYLEAQIDAFRKRTRAEKDAHDFMWGIAGNLDEAIIGSIAAYYASQPP
                     APGRSDDPALIAKGKELFDKGVPDRGIPACMACHGADAAGVADFPRLAGQHAKYVVKQ
                     LTYIQSLVRAAPVMHGIVKDLAPAEIQAVAAYVQSR"
     gene            complement(1029..>2192)
                     /locus_tag="VHT22_00765"
     CDS             complement(1029..>2192)
                     /locus_tag="VHT22_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3136107.1"
                     /translation="TVQAYAHEPEDRQAYGERVEAAFRAGVRRIRQRALLIAAVIFLV
                     FVAVGIILWIGGHDVLAGRLSAGQLASFVFYAVIVATSVGTVSEVAGELQRAAGATER
                     LMEILDTPAEIAAPAHPIPLPEPPLATVAIEQVTFAYPSRRNDPALSALSLSIAAGER
                     VALVGPSGAGKTTLFQLLLRFYDPQQGRVLIDGVDVRRADPRVVRGRIAVVPQDPVIF
                     AASVWDNVRYGRLDASDDEVRDACEAAYATEFIARLPRGFDTDLGERGVRLSGGQRQR
                     LAIARAVLSRRPILLLDEATSALDAESERMVQLALDRLMHGRTTLIIAHRLATVIGAD
                     RIVVLDGGIVVAEGTHQSLVRTDPLYRRLAALQFGFDAGAVDASPAIALAARG"
BASE COUNT          425 a          748 c          676 g          343 t
ORIGIN      
        1 attcgaacga caagctgcat acgttcaagg cgaaggcaac gtactaccac gaccgcaagt
       61 acggcgggac gctcgcctat ttctccacga cgggcagtgc tgacagcggc ctctatggca
      121 cgaacgcgga cggaaacgcg atcaaaccgg acagcaacgg ctacatcttc gagctcgact
      181 acctgccgat ccaaaacgtc cgcttgatgt ttcagtacac cgcgtacagc aaattcgacg
      241 gggcgtcgac caactacgac ggaaacggcc gcaatgcgcg ggacaacaac accgcgttct
      301 tcaacatatg ggtcgcattt tgaaccctca tgaagccggc gcagagtgtc cgcacccgca
      361 agtcgcatgg ataaaaaatc tggagactgt gctatgaatc gcaaacactt ggcgttcgcc
      421 tccatgcttt tcgcaggcgc ggttgcttcg gccggcgcgc aaaacaaccc ggcggacgtt
      481 cagaaggcgg cggaggaaaa ggcggtcaac ctgtgctcga cctgtcacgg cccgcgggga
      541 atcagcacgt ctcccgaatt tcccattctc gcagcgcaac gcaaaggcta tctcgaagcc
      601 cagatcgacg cattccggaa gcgaacccgc gccgagaagg acgcccacga tttcatgtgg
      661 ggaatcgcgg gcaatctcga cgaggcgatc atcggcagca tcgctgctta ctatgcctct
      721 caaccgcctg ctccgggccg aagcgacgat ccggcgctca ttgccaaagg caaggagcta
      781 ttcgacaaag gcgttcccga tcgaggcatt cccgcgtgca tggcatgcca tggcgcggat
      841 gctgccggcg tagcggactt tccgcggctg gccggtcagc acgccaagta cgtggtgaag
      901 cagctaacct atatccagtc gctcgtccgc gctgctccgg tcatgcacgg tatcgtcaag
      961 gatcttgccc ccgccgaaat tcaggcggtg gcggcctatg tgcaatccag gtagccgggc
     1021 atgattcatc agccccgcgc cgccagcgca attgcgggtg acgcatcgac cgcgccggcg
     1081 tcgaatccga attgcagcgc tgcgaggcgg cgatagagcg gatcggtgcg caccagcgat
     1141 tggtgggtgc cttccgcgac gacgatgccg ccgtcgagca cgacgatgcg atcggcgccg
     1201 atcaccgtcg ccaaccgatg cgcgatgatc agcgtcgtgc gcccgtgcat cagccgatcg
     1261 agcgccagct gcaccatgcg ctcgctctcg gcgtcgagcg cactagtcgc ctcgtccagt
     1321 agaaggatcg gccggcgcga cagcaccgcg cgcgcgatcg ccagccgctg ccgctgccct
     1381 cccgacagcc ggacgccgcg ctcgcccagg tcggtgtcga atccgcgcgg caggcgagcg
     1441 ataaactcgg tcgcataggc cgcctcgcag gcatcgcgca cctcgtcatc cgatgcatcc
     1501 aggcgaccgt agcgcacgtt gtcccagacg ctggcggcga agatcaccgg gtcctgcggc
     1561 accacggcga tccggccgcg cacgacgcgc gggtcggcgc ggcgcacgtc gacgccgtcg
     1621 atcagcaccc gcccctgctg cggatcgtaa aagcgcagca gcagctggaa cagcgtcgtc
     1681 ttgcccgcgc ccgacggtcc gaccagcgcc acgcgctctc ccgcggcgat gctcaagctc
     1741 aaggcgctca gtgccggatc gttgcggcgc gacggatagg caaaggttac ctgctcgatc
     1801 gcgaccgttg ccagcggcgg ttcgggcaga ggtatcggat gcgccggcgc cgcgatctcg
     1861 gccggggtgt ccaggatttc catcaggcgt tcggtcgcgc cggctgcgcg ctgcaattcg
     1921 ccggcgacct cgctcaccgt gcccaccgat gtcgcgacaa tcaccgcgta gaagacgaac
     1981 gatgcgagct gtcccgccga caaccgtccc gccagcacgt cgtgcccccc gatccaaagg
     2041 atgatgccga cggcgacgaa caccaggaag atcaccgcgg cgatcagcag cgcacgctgg
     2101 cggatgcggc gcactcctgc cctgaacgca gcctcgaccc gttcgccgta ggcttgccgg
     2161 tcctcgggtt cgtgcgcata ggcctgaacc gt
//
LOCUS       DATANS010000054         2078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_296556, whole genome shotgun sequence.
ACCESSION   DATANS010000054 DATANS010000000
VERSION     DATANS010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296556"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VHT22_00770"
     CDS             <1..729
                     /locus_tag="VHT22_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011455392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3136108.1"
                     /translation="NGIGAAIAQRLARDGYRIAAIDTDIESARVRAQALPDAVAYATD
                     VTDERAVEAMLDALGDAPDVLVNNAGIVRFGPLLEHSVADFRKVIDVNLVGTFIMSRA
                     VGRRMIERRSGAMVNITSINAVAPSPDAGAYPASKAAVALLTQHFALVLGPHGIRVNA
                     VAPGFIDAGMGAAIYADREVRESRSRSVPVGSLGTAEDVAEAVAFLVSDKARYTTGHQ
                     LMVDGGISFSLKNHLPRKPPPVRM"
     gene            801..1364
                     /locus_tag="VHT22_00775"
     CDS             801..1364
                     /locus_tag="VHT22_00775"
                     /inference="COORDINATES: protein motif:HMM:NF019462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136109.1"
                     /translation="MTATASTSTIRDCAQGIAAAIARHGGDGEKLGASIKASLRELVA
                     MPNLLELGIPREGNNVAFSQYLYFDGELSIIIFEVPNGKTIPPHDHGIWETLSVYRGR
                     IRHVVYERVDDQTVPGAAELRVAEDRELGPGDFAIVSPPADIHSFTALSDGTYGITVV
                     SGAYKRDRHYYQPEKNTYVIRQGRNAR"
     gene            1354..>2078
                     /locus_tag="VHT22_00780"
     CDS             1354..>2078
                     /locus_tag="VHT22_00780"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3136110.1"
                     /translation="MPADERGGAPIASRSPGIDSAAVIDVHAHVRLQATTGAAGRHGP
                     EFGTDENGKPWYRVGNYKLVGVKHTGSPFTDPDLRLKAMQEAQIDFQVLSPSPLTYFH
                     HIDLGEAMAFCRRHNDAMAELVRTYPGKLGGLAAVPMQDPTAAREELVRAVRELGLWG
                     AAIGTEFKEPLHSPALDPFYQTLVELDVPLFIHPAPAGIDGPPGDPNLKRFDLDIIVG
                     FGAQEAIAIATLIFGGVLDRHPKL"
BASE COUNT          384 a          669 c          664 g          361 t
ORIGIN      
        1 aacgggattg gagcggcgat cgcccagcgc cttgcgcgcg atggttaccg tatcgccgcg
       61 atcgacaccg acatcgagag cgcaagagtc cgggcccagg cgttgccgga tgccgtggcg
      121 tacgcgaccg acgtcaccga cgagcgcgcg gtcgaagcga tgctcgatgc gcttggcgat
      181 gctcccgacg tgctcgtcaa caacgcgggc atcgttcgat tcggtccgtt gctcgagcac
      241 agtgtcgcgg actttcgcaa ggttatcgac gtcaaccttg taggaacttt catcatgtcg
      301 cgggcggtcg gacgccgcat gatcgagcgc cgatcgggcg cgatggtcaa catcacttcg
      361 atcaacgctg tcgcgccgag ccccgacgcc ggcgcctacc cggcgtcgaa ggcggccgtc
      421 gcgttgctga cgcagcattt cgcgctggtg ctcggcccgc acggcatccg ggtcaatgcc
      481 gtggctcccg gcttcatcga cgcgggcatg ggagccgcaa tctatgccga tcgtgaagtg
      541 cgtgaatcgc gaagcagaag cgtaccggtg ggctcgctcg ggacggccga agacgttgcc
      601 gaagctgttg cctttctggt gtccgataaa gcccgctata ccaccgggca tcagctgatg
      661 gtggacggtg gaatctcgtt cagcctgaaa aatcacctgc cgcgaaaacc gccgccggtg
      721 cgcatgtaat cgacggttgg gggcattacg aaccgtaaga acatgacggc attttgggac
      781 aaccggcagg acagttaacc atgactgcga cagcgtcgac ttcgactatt cgcgattgcg
      841 cccaggggat cgcggctgcc attgcccggc acggcggcga cggtgaaaaa ttgggagcgt
      901 cgatcaaggc gtcgctgcgc gagcttgtgg cgatgccgaa cctgctggag ctcggcatcc
      961 cgcgcgaggg caacaatgtc gcgttctcgc agtatctcta tttcgacggg gagctttcta
     1021 tcatcatttt cgaggttccc aacggaaaga caataccgcc gcacgaccat ggcatctggg
     1081 agacattgtc cgtgtatcgc ggacggattc gacacgtcgt ctacgagcgc gtcgacgacc
     1141 agacggtgcc gggagcggcc gagcttcggg tcgcagagga ccgcgagctg ggtcccggcg
     1201 acttcgcgat cgtctctccg cccgcggata tccacagctt cacggcgctc agcgacggga
     1261 cctacggcat caccgtcgtc agcggagctt acaagcgcga tcgtcactac tatcagccgg
     1321 aaaagaacac ctacgtgatc aggcagggac gcaatgcccg ctgacgagcg cggaggcgcc
     1381 cccatcgcga gccggtcgcc aggaatcgac agcgctgcgg tcatcgatgt ccacgcccat
     1441 gtgcggctgc aggcgaccac cggcgccgcc ggccggcatg gaccggagtt cggcaccgac
     1501 gagaatggca agccctggta ccgcgtcggc aactacaagc tcgtcggcgt caagcacacc
     1561 ggcagcccgt tcaccgaccc ggatctgcgc ctgaaggcca tgcaggaggc gcagatcgac
     1621 tttcaggtcc tgtcgccgag tccgctgact tactttcacc atatcgacct gggagaagcc
     1681 atggcgtttt gccggcgcca taacgacgcc atggcggagc ttgtgcgaac ttatcccggc
     1741 aagctcggcg gcctcgcggc cgtgccgatg caggacccga ccgccgcgcg cgaagaactg
     1801 gtgcgcgccg ttcgcgagct cgggctatgg ggagcggcga tcggcaccga gttcaaggag
     1861 ccattgcaca gcccggccct cgacccgttc taccagacgc tggtcgagct cgacgtgccg
     1921 cttttcattc atcccgcgcc tgccggtatc gacggaccgc cgggcgatcc gaatctcaag
     1981 cgcttcgacc tggacatcat cgtcggcttc ggcgcgcagg aggcgatcgc gatcgccacg
     2041 ctcattttcg gcggcgtcct cgatcgtcac ccgaagct
//
LOCUS       DATANS010000055         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_633329, whole genome shotgun sequence.
ACCESSION   DATANS010000055 DATANS010000000
VERSION     DATANS010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633329"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /locus_tag="VHT22_00785"
     CDS             complement(<1..1454)
                     /locus_tag="VHT22_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3136111.1"
                     /translation="MQYDYIIVGAGSAGCVLANRLTADGRYSVLLLEAGPPDTYPWIH
                     VPIGYAKTMFDARYNWCFYTEPDPGMNGRKIYWPRGKTLGGSSSINGLIYVRGQREDY
                     DRWAAAGNAGWSYGEVLPYFKRLEHSAGGDPAYHGRDGPLWCSPIGRRHELIDAVIAG
                     AGELGIARNDDFNGPVQEGAGYYHLSTRRGWRCSTADAYLKPAQRRQNLRIETGAQAA
                     RIAFNGRRAGGVSYRRRGAEHIARARREVLLCAGAVQTPQLLQLSGVGPEALLRSFDI
                     PVMHDLPGVGENLQDHLQARVIYECTRPITTNDDLASFWRTIGMGIDYLFTRDGPMAV
                     GINQGGIFARACPDAATPDVQFHIATLSSDMAGSPTHTFSGFTMSVCQLRPQSRGWVR
                     IRSADPLTAPAMQANYLSTQADRETLIAGIRLARRLVATGALSPYVKCEYRPGPAAES
                     DADLLEFARNTGGTIFHPSGTCKMGDASDHQAVV"
BASE COUNT          250 a          492 c          535 g          268 t
ORIGIN      
        1 tcgaccactg cctggtggtc gctggcgtcg cccatcttgc aggtgccgct gggatggaag
       61 atcgttccac cggtgttccg ggcgaattcc agcagatccg cgtcggactc agccgccggc
      121 ccgggcctgt attcgcactt gacgtacggc gagagcgcac cggttgccac gaggcggcgc
      181 gcaagccgga ttccggcaat cagcgtctcc cggtcggcct gcgtcgacag gtaattcgct
      241 tgcatcgcgg gcgcggtcag cggatcggcc gagcggatgc gcacccaccc tcgcgattgc
      301 ggccgaagct ggcagaccga catcgtgaac ccggaaaagg tgtgtgtcgg cgagccggcc
      361 atgtcggacg aaagggtagc gatatggaat tgcacgtccg gcgtcgccgc gtccgggcac
      421 gcgcgcgcga atatgccgcc ctggttgatg ccgacggcca tcggtccgtc acgcgtgaac
      481 agatagtcga tacccatgcc tatcgtgcgc cagaagctgg cgaggtcgtc gttggtcgtg
      541 atcggccttg tgcattcgta gatgacgcgc gcctgcaggt ggtcctgcag gttttcgccg
      601 acgccgggga gatcgtgcat gacgggaatg tcgaagctgc gcagaagagc ctccggcccc
      661 acgccggaga gttgcagcag ctgcggagtt tgcaccgcac cggcgcaaag caggacttcg
      721 cgtcgagcgc gcgcgatgtg ctcggcgccg cgtcggcgat agctgacgcc accggcgcgg
      781 cgaccgttga acgcgatccg cgccgcctgt gcgccggtct cgatgcggag gttctgccgc
      841 cgctgcgccg gtttgagata ggcgtcggcg gtgctgcatc gccatccgcg gcgggtcgac
      901 aaatgataat agccggcgcc ttcctgcacg gggccgttga aatcgtcgtt gcgggcgatg
      961 ccgagctcgc cggcgccggc gatgaccgcg tcgatcaatt cgtgcctgcg gccgatcggc
     1021 gagcaccaca gcggtccgtc gcgtccgtga tatgccggat cgccgccagc cgagtgctcg
     1081 aggcgcttga aatagggcag cacctcgcca tagctccagc cggcgttgcc cgccgctgcc
     1141 cagcgatcgt agtcttcccg ctgaccgcgc acatagatga gcccgttgat cgagctcgag
     1201 ccgccaagcg tcttgccgcg cggccagtag atcttccggc cgttcatgcc gggatcgggc
     1261 tcggtgtaga agcaccagtt gtagcgtgcg tcgaacatcg ttttggcgta tccgatcggc
     1321 acgtggatcc agggataagt atccggcggc cccgcctcga gcagcagcac cgagtagcgg
     1381 ccgtcggcgg tcaggcggtt ggcgagcacg caaccggccg atcccgcgcc cacgatgatg
     1441 tagtcgtact gcattcggcg gattctacgc aagcatggcc gggcgggtgc ccgtagggcg
     1501 cgcagtaatc cgggcaaaac gaggcctggt ttgacacttg ggaat
//
LOCUS       DATANS010000056         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_800698, whole genome shotgun sequence.
ACCESSION   DATANS010000056 DATANS010000000
VERSION     DATANS010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800698"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VHT22_00790"
     CDS             complement(<1..198)
                     /locus_tag="VHT22_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX3136112.1"
                     /translation="MSSYIGALARCLAACALALSFSASAQQAITLHGAVQFNDDHAFN
                     RALVRFQELTAKYYGKPINFVL"
     gene            395..796
                     /locus_tag="VHT22_00795"
     CDS             395..796
                     /locus_tag="VHT22_00795"
                     /inference="COORDINATES: protein motif:HMM:NF022610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HEX3136113.1"
                     /translation="MTRRCRLAVTLVTLALHLLAPVGAHAAARPAADSSDYCTAARYD
                     VQSGTGALAPQRAASADSGVPAPRPGQSPHSHCPSCLGTFVVVAIPPSAAPLVATPRS
                     LDSVPTDAARTVIAAQPALLPPLRGPPSLLF"
     gene            902..1375
                     /locus_tag="VHT22_00800"
     CDS             902..1375
                     /locus_tag="VHT22_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX3136114.1"
                     /translation="MTRILAIAAVAAIAVASGASAHEYQLRALRIDHPFARATPPGAR
                     SGGVFLSVENKGDRTDRLLTVSTPVAGTAELHQMVMDAGVMRMRAVAGLDVKPGDRLV
                     LQPGAYHVMLTDLKRPLQAGETFPLTLGFEKAGSIKVDVEVESMAGGAMHPHTVR"
     gene            1433..>1783
                     /locus_tag="VHT22_00805"
     CDS             1433..>1783
                     /locus_tag="VHT22_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136115.1"
                     /translation="MNTKNRRSIATPLTLVAAATAVLSAESAAAHGLAGKRFFPATLA
                     TDDPFVADELSLPTIESRKMPASGDEAATRETSYSVDVSKRLTDNLGIGFGAVYLVLQ
                     PDGSDTQRGFDNLAA"
BASE COUNT          307 a          597 c          582 g          297 t
ORIGIN      
        1 caggacgaag ttgatcggct tgccgtaata cttggcggtg agctcctgga agcgcacgag
       61 agcgcggttg aacgcatggt cgtcgttgaa ctggacggcg ccgtgcaggg tgatcgcttg
      121 ttgcgcggat gcactgaagg acagtgcaag agcacatgcg gcaaggcagc gcgcaagcgc
      181 gccgatatag gaagacatcg cttctcctcc gggttgtggt ctattaaacg gcccggatct
      241 gcgcccggag ccgccggtac gataacgaac catctggtaa ggtggtcaga ccgcattcaa
      301 cgctttggcc ggcgaagtgt caatgccagc tttgcacaaa gaccgtttca ggattcagca
      361 atcgtccgcc aacgcgcggt gtagaatcgt cgcgatgaca cgacgctgcc gcctcgcagt
      421 cacgctcgtc accttggcgc tgcatttgct cgcgccggtg ggtgcccatg cggcggcccg
      481 gccagccgcc gattcgagcg actactgcac ggcggcgcgc tatgacgttc agtcgggtac
      541 cggcgcgctc gcaccgcagc gtgcggcgag cgcggattcc ggcgttcccg cgccccgacc
      601 gggccagtcc ccgcattcgc actgcccatc gtgtctgggc acgttcgtcg tcgtggcgat
      661 cccaccttcg gcggcgccgc tcgttgcgac tccgcgctcg ctcgacagcg tgccgacgga
      721 tgcggcccgc accgtcatcg cagcgcaacc tgcgctgctg ccgccgctgc gcggtcctcc
      781 ctcgctcctc ttctaggctt ccaccgaccg cggccggcaa atcggccgcg gcttgcggtc
      841 gatcgcgcgt gcgcaccgcg gcatgcgcga tcgatcgcat tcgaatccaa cggggaccgt
      901 catgacaaga atcctggcca tcgcggccgt cgccgcaatc gctgttgcga gcggcgcatc
      961 ggcacacgag tatcaactgc gagcgctgcg catcgaccat ccgtttgccc gcgcgacgcc
     1021 tcccggagcg cgctccggcg gcgttttcct gagcgtggaa aacaagggcg atcgaaccga
     1081 taggctgctc accgtatcga cgccggtggc cggcaccgcc gagctgcatc agatggtgat
     1141 ggatgccggc gtgatgcgca tgcgcgcggt cgcgggtctc gacgtcaagc ccggcgaccg
     1201 gctggtgctg cagcccggcg cttatcacgt gatgctcacc gacctcaagc gaccgctgca
     1261 ggcgggcgaa acctttcccc tgacgcttgg gttcgaaaag gcgggatcga tcaaggtcga
     1321 cgtcgaggtc gagagcatgg caggcggcgc catgcatccc cataccgtgc gttgaaccga
     1381 acattcgcgc ctgccgcatc ggtgacatgc ggccaatgaa agggacgtat ccatgaacac
     1441 gaaaaataga cgatcgatcg ccacgccgct gacgctggtc gcggcagcga cggccgtcct
     1501 gtcggcggaa tccgcggcgg cgcacgggct cgccggcaaa cgtttcttcc cggcgacgct
     1561 ggcgaccgac gatccgttcg tcgccgacga actgtcgctg ccgacgatcg aaagccgcaa
     1621 gatgccggcg agcggcgacg aggcggcaac acgcgagacc agctattcgg tcgacgtgtc
     1681 caagcgtctc accgacaacc tgggcatcgg tttcggtgcg gtgtacctcg tgctgcagcc
     1741 cgacggcagc gacacgcagc gcggcttcga caacctggcg gcg
//
LOCUS       DATANS010000057         4091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_591194, whole genome shotgun sequence.
ACCESSION   DATANS010000057 DATANS010000000
VERSION     DATANS010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591194"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VHT22_00810"
     CDS             complement(<1..269)
                     /locus_tag="VHT22_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004548608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136116.1"
                     /translation="MSPFETGTPKAEARMRGEPRGLLVPGRNCWRLAHATRVGFLVDG
                     SEYFGAVRAALAGAQRDIFILGWDIDSRMWLVPGGANDGFPEPLR"
     gene            633..2579
                     /locus_tag="VHT22_00815"
     CDS             633..2579
                     /locus_tag="VHT22_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3136117.1"
                     /translation="MKSAQDSTFPRLLFEHARVRGDRPATREKDLGIWQTWTWSQVAA
                     DVRALACGLAAQGFQRGMHLAIIGDNRPRLYWAMAAAQAIGGIPVPMYQDAPAAEFVY
                     VLNDAEIGYALAEDQEQVDKVLEAKPQVPTLQHVYFDDSRGLRNYENVSSLDRLLDIG
                     REFDRAHPGFFDAEVAKGRPDDVSIMLYTSGTTGKPKGVCQTHAAFIVAAQGGCAFDK
                     LGPGDSILAYLPMAWVGDHLFSYAQWLVAGFTINCPESGDTVMTDLREIGPSYYFAPP
                     RIFENLLTQVMIRMEDASAAKRWLFRYFTAVARRCGSEILDGKPVGMLDRALYAIGNL
                     VVYGPLRNVLGMSRIRVAYTAGAAIGPDLFRFYRSIGINLKQLYGSTETCAYVCLQPD
                     GGVRFDTVGQAAPGVEIRIAENGEVLVRGPMLLKEYYKRPDATLESIDAAGYFRTGDA
                     GFFDDAGQLKIIDRAKDVGKLATGAMFAPNYIENKLKFFPHIREAVAFGNERDKVCAF
                     INIDMGSVGNWAERRGIPYSGYTDLAAKAEVQALIRECVEQVNAELAQEGSLADSQVQ
                     RFLILHKELDPDDDEMTRTRKVRRGFVAEKYASLIEALYSGRTVQHVETQVKFEDGRT
                     GIVAADLTIAEAKTFAANAAKAAA"
     gene            2591..3379
                     /locus_tag="VHT22_00820"
     CDS             2591..3379
                     /locus_tag="VHT22_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3136118.1"
                     /translation="MTAVRAIGDSVLRLDGVSLAFGGVKALNDISLDVREHEIRAIIG
                     PNGAGKSSLLNVINGVYHPQQGTITFRGKPRHDMDTHETAALGIARTFQNIALFRGMS
                     VLDNIMTGRNLKMKATVLEQAIWFGRARREELENRAKVEEVIDFLEIQHIRKTPVGRL
                     PYGLQKRVELARALAAEPSLLLLDEPMAGMNVEEKQDMCRFVLDVNEQYGTTVVLIEH
                     DMGVVMDISDRVVVLDYGRKIGDGTPDEVRNSKTVIDAYLGVPH"
     gene            3394..>4091
                     /locus_tag="VHT22_00825"
     CDS             3394..>4091
                     /locus_tag="VHT22_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX3136119.1"
                     /translation="MQFFFEVLIGGLLSGVMYSLVALGFVLIYKASGVFNFAQGSLVF
                     FAALTFVGLMEHGLPFGLALLVTLLAMILLGLLIEKFVLRPLVNQPQISLFMATIGLS
                     FLIEGLAQLLWGANVRGLDIGIQDEPLAWIADPTGINVSKFDLAAAGIAAVLVIMLAL
                     FFSRTRIGRALRAVADDHQAALAVGIPLQQIWAIVWAVAGFVALVAGLLWGARNGVQF
                     ALTFVALKALPVLIL"
BASE COUNT          713 a         1315 c         1337 g          726 t
ORIGIN      
        1 tcgcgcaggg gctcgggaaa gccgtcgttc gcgccaccgg ggactagcca catgcggctg
       61 tcgatgtccc agccgagaat gaatatgtcg cgctgcgcgc cggcaagcgc cgcgcggacc
      121 gcaccgaaat actcgcttcc atcgaccagg aatccgaccc gtgtcgcgtg cgccaggcgc
      181 cagcaattgc gcccaggcac gagcagaccg cgcggttcac cgcgcatccg ggcctcggcc
      241 ttgggggtgc cggtttcgaa tggcgacatc gttgccggcg cgtcgttcac gcggctggcc
      301 tccgccatcg cttgcgagaa ctgcggcaaa cgccatcaac gcagtttggg ggcatggtcg
      361 ttcctccgtc acgccgcagc ccgtcgctgc ggacggtcac gacgtttcac tggaagcaag
      421 gagggtgccg cggcagaagt cattgtttat caagcgcaag ccgagaccgt cggccacggc
      481 cctcatgcaa gaactgctcg cccgggtaag aattgcccgc ggcccacctc ctgccgatcg
      541 ccttggttgc gcctgcggcg gcggcaggta ttatgacctc ccggcaagac tggccacccg
      601 cccgcaaggc ttcgaggggg agctccggcg aaatgaagtc cgcgcaagac tcgacgtttc
      661 cccggttgct gttcgagcac gcacgcgtgc gcggcgatcg tccggcgacc cgcgaaaagg
      721 acttgggcat ctggcagacc tggacatgga gccaggtggc ggccgacgtg cgtgcgctcg
      781 cctgcggtct tgccgcgcag ggtttccagc gcggcatgca cctggcgatc atcggcgaca
      841 accggccgcg gctctactgg gcgatggccg ccgcgcaggc catcggggga atcccggttc
      901 cgatgtacca ggacgcgccg gccgcggaat ttgtctatgt gctgaacgac gctgaaatcg
      961 gctatgcgct ggccgaggac caggagcagg tcgacaaggt actcgaggcg aagccgcagg
     1021 ttccgaccct gcagcacgtc tacttcgacg attccagggg cttgcgcaac tacgagaacg
     1081 tgtcgagcct cgatcgtctg ctcgacatcg gccgcgaatt cgaccgcgcg catcccggat
     1141 tcttcgacgc ggaagtggcc aagggccggc ccgacgatgt gtcgatcatg ctttacacgt
     1201 cgggaacgac cggcaagccg aagggcgtgt gtcagacgca cgcggctttc atcgtcgcgg
     1261 cgcaaggcgg ctgtgcgttc gacaagctcg gccccggcga cagcatcctc gcctatctgc
     1321 cgatggcgtg ggtcggcgac catctgtttt cctacgcgca gtggctcgtc gcgggcttca
     1381 cgatcaactg tcctgaatcg ggggacacgg tgatgaccga cctgcgcgag atcgggccca
     1441 gctactattt cgccccgccg cgcatcttcg agaacctgct gacgcaggtg atgatccgga
     1501 tggaggatgc atcggccgcc aagcgctggc tgttccgcta tttcaccgcg gtcgcgcgcc
     1561 gctgcggctc cgagatcctc gacggcaagc cggtcggcat gctcgatcgt gcgctctacg
     1621 cgatcggaaa cctggtcgtg tacggcccgc tgcgcaacgt gctcggcatg agtcgcatcc
     1681 gcgtggcgta caccgcgggc gcagcgatcg gccccgactt gttccgcttc taccgctcga
     1741 tcggaatcaa cctgaagcag ctctacggtt cgaccgagac ctgcgcctat gtctgccttc
     1801 agcccgatgg cggcgtcaga ttcgacaccg tcggacaggc cgcgcccggt gtcgagatca
     1861 ggattgccga aaacggtgaa gtgctggtgc gcgggccgat gctgctcaag gagtactaca
     1921 agcggccgga cgcgacgctc gaatcgatcg acgccgccgg ctacttccgt accggggatg
     1981 ccggcttttt cgacgacgcc gggcagctga agatcatcga ccgcgccaag gacgtcggaa
     2041 agctggcgac cggcgcgatg ttcgcgccga actacatcga gaacaagctc aagttctttc
     2101 cgcatatccg ggaagcggtc gctttcggca acgagcgcga caaggtttgc gcgttcatca
     2161 acatcgacat gggctcggtc ggcaactggg ccgagcggcg cggcattccg tactccggct
     2221 ataccgatct tgccgccaag gcggaagtcc aggccttgat ccgcgagtgc gtcgagcagg
     2281 tcaacgccga gctcgcccag gaaggctcgc tcgccgattc gcaggtgcag cggttcctga
     2341 tattgcacaa ggagctcgat cccgacgacg acgagatgac gcgcacgcgt aaggttcgtc
     2401 gcggcttcgt cgccgaaaag tacgcgtcgc tgatcgaggc actctattcg ggcaggaccg
     2461 tgcagcacgt cgagacccag gtgaagttcg aggacggacg caccggcatc gttgccgccg
     2521 acctcaccat cgccgaggcc aaaacgtttg ccgcgaacgc ggcgaaggca gcggcctagc
     2581 gcccgcgaac atgacggccg tccgcgcgat cggcgactcg gttctgcgtc tcgacggcgt
     2641 atcgctggct ttcggaggcg tcaaggcgct caacgatatt tccctcgacg tgcgcgagca
     2701 cgagatccgg gcgatcatcg gccccaacgg cgcggggaag agctcgctgc tcaacgtcat
     2761 caacggcgtc taccacccgc agcaggggac gatcaccttt cgcggcaagc cgcggcacga
     2821 catggacacg cacgagacgg cggcactcgg catcgcgcgc acgttccaga acatcgcgct
     2881 gttccgcggc atgagcgtgc tcgacaacat catgaccggg cgcaatctga agatgaaggc
     2941 aacggtcctg gagcaggcga tctggttcgg gcgcgcccgg cgcgaggagc tcgagaatcg
     3001 ggcgaaggtc gaggaggtca tcgatttcct ggagattcag cacatacgaa agactccggt
     3061 cggcaggctg ccgtatgggt tgcagaaacg cgtcgagctc gcgcgcgcgc tcgcggccga
     3121 accttcgctg ctgctgctcg acgagccgat ggccggaatg aacgtcgagg aaaagcagga
     3181 catgtgccgc ttcgtgctgg acgtcaacga gcaatacggc acgacggtcg tgctgatcga
     3241 gcacgacatg ggggtcgtga tggacatttc cgaccgggtc gtcgtactgg actacggccg
     3301 caagattggc gacggcacgc ccgacgaggt gcgcaacagc aagaccgtga tcgacgccta
     3361 tctcggcgtg ccgcattaac cggcgacaag gcgatgcaat tcttcttcga agtcctgatc
     3421 ggcggcctgc tttccggcgt catgtattcg ctggtcgcgc tgggctttgt gctgatctac
     3481 aaggcctcgg gcgttttcaa tttcgcccag ggctcgctgg tctttttcgc ggcgctgacc
     3541 ttcgtcgggc tgatggaaca cggtttgccg ttcggcctcg cgctgctagt gaccctgctg
     3601 gcgatgatcc tgctcggcct tctgatcgaa aaattcgtcc tgcgcccgct ggtcaaccag
     3661 ccgcagatct cgctgttcat ggctacgata ggcttgagct tcctgatcga aggcctggcg
     3721 caactgctgt ggggcgccaa cgtgcgcggc ctcgatatcg gcatccagga cgagccgctg
     3781 gcgtggattg ccgacccgac gggcatcaac gtgtcgaagt tcgacctcgc ggccgcaggc
     3841 atcgcggcgg tcctggtcat tatgctggcg cttttcttca gccgcacccg catcggccgc
     3901 gcgctgcgag cagtcgccga cgatcaccag gcggcgctgg ccgtgggcat cccattgcag
     3961 cagatctggg cgatcgtctg ggcggtcgcc ggattcgtag cactcgtcgc cggccttttg
     4021 tggggcgcgc gcaacggcgt ccagtttgcg ctgaccttcg ttgcgctgaa ggcgctcccg
     4081 gtgctgatcc t
//
LOCUS       DATANS010000058         3784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_171510, whole genome shotgun sequence.
ACCESSION   DATANS010000058 DATANS010000000
VERSION     DATANS010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171510"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VHT22_00830"
     CDS             complement(<1..307)
                     /locus_tag="VHT22_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136120.1"
                     /translation="MPNSGRSIAALRVLRLLPLLMLMLGLAPAPAGAQALYERFIQSV
                     TIDRSDEVAALLGRGMDPNTVAPNGDPVLVIAARSGFEPTLDALLAAGAKVNIRNPYG
                     "
     gene            complement(297..1073)
                     /locus_tag="VHT22_00835"
     CDS             complement(297..1073)
                     /locus_tag="VHT22_00835"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX3136121.1"
                     /translation="MFVDSHCHINFPELAADLPAVFEAMAGNRVTHAMCIGVNLPDLP
                     QVLALADEHANIYASVGVHPDYEDTPEPSIEQLIALAARPKVAAIGETGLDYYRATGD
                     LEWQRQRFRTHIRAARSCGKPLVIHTRSAAADTLAIMRDERAGDVGGVMHCFTETWDV
                     AQGALELGFHISFSGIVTFKNAVALKDVARHVPLDRMLIETDAPYLAPVPYRGKLNQP
                     AYVRYVAEEIARLREVPIDVIAGATSANFFRMIRTSAHAE"
     gene            complement(1076..1420)
                     /locus_tag="VHT22_00840"
     CDS             complement(1076..1420)
                     /locus_tag="VHT22_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX3136122.1"
                     /translation="MLNRPGVLSLNIREKAALYAAYMPFLKGGGIFIPTNRAYQLGEE
                     VFMLLSLMDDPQRIAVQGRVVWITPEGVQGNRTQGIGVQFTPDETGASARATIERLLG
                     ETLASNRPTHTM"
     gene            complement(1528..2553)
                     /gene="holB"
                     /locus_tag="VHT22_00845"
     CDS             complement(1528..2553)
                     /gene="holB"
                     /locus_tag="VHT22_00845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX3136123.1"
                     /translation="MAAAQALLPWHRELATAMLQQRSRWPQALLIAGRRGLGKRALAL
                     HFARALLFEAPRPDGDACGVCPSCLYVAVGAHPDLRLIEPLLLDEEGNVIVVDAITVD
                     RIRELTAFTQLSTHRHRAKVAVIAPAEAMNPAAANALLKTLEEPPADTYLMLVSHQSA
                     RLPATIISRCQRLPVPEPDAASAVAWLRTQGVSDADQVLAQAGGAPLLALELADPLVQ
                     RECASLLADLARPERLSPVAVGARLDAAKKEERKPLLAAAVYWLLTWTADLASVASGG
                     PPRFHPQRRAALAELATRVARVPLFRYYQSLLQQRALLSHPLAPRLVAEALLIDYRDL
                     FIRGRAD"
     gene            complement(2574..3194)
                     /gene="tmk"
                     /locus_tag="VHT22_00850"
     CDS             complement(2574..3194)
                     /gene="tmk"
                     /locus_tag="VHT22_00850"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX3136124.1"
                     /translation="MTTDSSRGRLITVEGIDGAGKSTHLPWLKQSLEGRGRQAWLTRE
                     PGGTPLGERLRDLLLHQPMGELTETMLMFAARREHCEREIWPRLDQGVWVVCDRFSDA
                     TYAYQGGGHGVSAECIAALERLSLGDFRPDLTLLFDVPVEVGRERLADGRPLDKFERQ
                     QPGFFERVRTAYLTRAAAEPSRFRIIDSTRPQADVRVDLAAIIAAL"
     gene            complement(3244..>3784)
                     /gene="mltG"
                     /locus_tag="VHT22_00855"
     CDS             complement(3244..>3784)
                     /gene="mltG"
                     /locus_tag="VHT22_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX3136125.1"
                     /translation="PEGLFFPDTYFFASGSTDTALLKRAYRLMQERLDAAWASRASGL
                     PLANPYEALTLASIVEKETGRAADRPLVAAVFINRLKIGMRLQTDPAVIYGIGERFNG
                     NLKKSDLENDHAFNTYVRSGLPPTPIALPGQASIDAVLHPASVPYLYFVARGDGSSEF
                     SSNIADHNRAVAKYQRGGR"
BASE COUNT          660 a         1229 c         1270 g          625 t
ORIGIN      
        1 cgccgtacgg atttctgatg ttgaccttgg cgcccgccgc cagcaacgcg tcgagcgtcg
       61 gctcgaaccc cgagcgcgcc gcgatcacca gtaccgggtc gccattggga gcgacggtgt
      121 tcggatccat gccgcgcccg agcagggcgg cgacttcgtc cgaccggtcg atcgtgaccg
      181 actggatgaa gcgctcatag agggcctgcg ccccggccgg cgccggcgcg agcccgagca
      241 tgagcatgag cagcggcaac aaccgcagta cgcgcagcgc ggcgatgctg cggccgctat
      301 tcggcatgcg cgctggtccg gatcatccgg aaaaaattgg ccgacgttgc gccggcgatc
      361 acgtcgatcg gcacctcacg cagccgcgcg atttcctcgg cgacgtaacg cacgtaggcc
      421 ggctggttga gcttcccccg atacggcacc ggcgccaggt agggtgcatc ggtttcgatc
      481 agcatgcggt ccaggggcac gtgtcgcgca acatccttga gcgcaacggc attcttgaac
      541 gtgacgatgc cggaaaacga gatgtggaat cccaattcga gcgcgccctg cgcgacatcc
      601 caggtttcgg tgaagcagtg cattacgccg cccacgtcgc ccgcgcgctc gtcgcgcatg
      661 atggcaagcg tatcggccgc ggcggagcgc gtgtggatca ccagtggttt gccgcaggac
      721 cgtgccgcgc gaatgtgcgt gcgaaagcgc tggcgctgcc actcgagatc gccggtggcc
      781 cggtaatagt cgagaccggt ctcgccgatt gctgcgacct tcggccgcgc ggcgagcgcg
      841 atcagctgct cgatggaggg ctcgggcgtg tcctcgtagt cgggatgcac gccgacgctc
      901 gcatagatat tcgcatgctc gtcggccagc gccagcactt gtgggagatc gggcaggttg
      961 acgccgatgc acatcgcgtg ggtgacgcga ttcccggcca tcgcttcgaa taccgccggc
     1021 aggtccgccg ccagctccgg gaaattgatg tggcagtgcg aatcgacgaa cacaactaca
     1081 tcgtatgcgt cggccgattc gaggcgagtg tctcgccgag cagcctttcg atcgtcgccc
     1141 gcgccgacgc gccggtctcg tccggggtga attgaacgcc gatgccctgc gttcggttgc
     1201 cctgcacgcc ttcgggcgta atccagacca ccctgccctg cacggcaatc cgctgcggat
     1261 catccatcag cgacagcagc atgaagactt cctcgcctag ctgataggcc cgattggtcg
     1321 gaatgaatat gccgccaccc ttgagaaagg gcatgtatgc cgcatatagc gcggcctttt
     1381 cgcgaatgtt gagcgaaagc acgccgggcc ggttgagcat agcccgtgtt gcagtgttgg
     1441 gggagcgatc cgccgccatg ggtcccgagc tcaggttgat tgttgatcgc gccgtggcga
     1501 tgaagtttgg ccgagcggtc gctgcagtca atcggcccgg ccgcgtatga acagatcgcg
     1561 ataatcgatc agcagcgcct ccgcgaccag ccgcggcgcc agcggatgcg acagcagcgc
     1621 gcgttgctgc aacagcgact gataatagcg gaacagcggg acccgcgcca cacgagtcgc
     1681 cagctccgcc agcgcggcgc gccgttgcgg atgaaagcgc ggcgggccgc cgctggccac
     1741 cgatgcaagg tcggccgtcc aggtcagcag ccagtacacc gcggcagcaa gcagcggctt
     1801 gcgctcttcc ttcttggctg cgtccaggcg ggcgccgacc gcaaccggcg acaggcgctc
     1861 cggccgcgcg agatcggcca gcaagctggc gcactcgcgc tgcaccagag gatccgcgag
     1921 ctcgagcgcc agcaaaggcg cacccccggc ctgcgccagg acctggtcgg cgtcggagac
     1981 gccttgcgtc ctgagccatg ccaccgccga tgcagcgtcc ggttcaggca ccggcaggcg
     2041 ctggcagcgg ctgatgatcg tcgctggcag acgcgcgctc tgatggctga ccagcatcag
     2101 ataggtgtcc gccggaggct cctccagcgt tttcagcaag gcgttggcgg cggcggggtt
     2161 catcgcttcg gccggtgcga ttaccgcgac cttggcgcga tgtcgatggg tcgagagttg
     2221 cgtgaatgcg gtgagctcgc gaatgcgatc gacggtgatg gcgtcgacga ctatgacgtt
     2281 tccctcttcg tctaagagca gcggctcgat cagccgcaaa tccggatgcg cgccgaccgc
     2341 cacgtacagg cagctgggac aaacgccgca cgcatctccg tcaggacgcg gggcttcgaa
     2401 cagcagtgcg cgtgcaaagt gcaaggcgag cgcgcgcttg cccaagccgc gcctgccggc
     2461 gatcagcaag gcctgcggcc atcgcgaccg ctgttgcagc atcgcggtcg cgagttcgcg
     2521 atgccacggc agcaaggctt gtgcggcagc caacggaggc ggcgtcgatt cgctcacaag
     2581 gcggcgatga tcgcggccag atcgacccgc acgtcggcct gaggccgcgt gctgtcgatg
     2641 atgcgaaagc gcgatggctc ggccgccgcg cgagtcagat acgccgtgcg gacgcgctcg
     2701 aagaatccgg gttgctgccg ctcgaatttg tccaacggcc gtccgtcggc cagtcgctcg
     2761 cgtcccacct cgaccggtac gtcgaacagc agcgtcagat caggacggaa gtcacccagg
     2821 ctcagccgtt cgagcgctgc gatgcattcc gccgaaacgc catggccgcc gccctggtag
     2881 gcgtaagtgg catcgctgaa ccggtcgcag acgacccaca ctccctggtc gagccgcggc
     2941 cagatctcgc gctcgcaatg ttcgcgccgc gcggcgaaca tcagcatcgt ctcggtcagc
     3001 tctcccatcg gctgatgcag cagcaggtcg cgcagtcgtt cgcccagcgg cgtgccgccc
     3061 ggctcgcgcg tcagccaggc ctgccggccc cggccctcca gcgactgctt gagccatggc
     3121 agatgcgtgc tcttgccggc gccgtcgatg ccttcgacgg tgatcaggcg gccgcgggat
     3181 gaatcagtcg tcaagggaag gcagggatcg gcgcaacaaa gagcgaagca atgcgaagga
     3241 acgctagcgt ccgccgcgct gatattttgc cacggcgcga ttgtgatcgg cgatattgct
     3301 cgagaattcg ctcgagccgt cgccgcgcgc gacgaagtaa aggtaaggta ccgatgccgg
     3361 atgcaggacc gcgtcgatcg acgcctgtcc gggcaaagcg atcggcgtcg gcggcagacc
     3421 gctgcggacg taggtattga acgcgtgatc gttttcgaga tcactcttct tcaggttccc
     3481 gttgaaacgc tcgccgatgc cgtagatcac tgcgggatcg gtttgcagcc gcatgccgat
     3541 cttcagacgg ttgatgaaca cggccgcgac cagtggccgg tccgcggcgc gaccggtttc
     3601 cttttccacg atcgatgcga gcgtcagcgc ttcgtaggga ttcgccagcg gtaagccgct
     3661 cgcgcgggaa gcccatgccg cgtccaggcg ctcctgcatc aggcgatagg cgcgcttcag
     3721 caatgcggtg tcggtcgatc cgctggcaaa aaagtaggtg tccgggaaaa aaaggccctc
     3781 gggg
//
LOCUS       DATANS010000059         1659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_171729, whole genome shotgun sequence.
ACCESSION   DATANS010000059 DATANS010000000
VERSION     DATANS010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171729"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..953
                     /locus_tag="VHT22_00860"
     CDS             180..953
                     /locus_tag="VHT22_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3136126.1"
                     /translation="MARILAITNQKGGVGKTTTGVNLAASLAATKQRVLLIDLDPQGN
                     ATMGSGVDKRALKVTVYHLLLGEAEIGAVRVRSTSGNYDVLPANRELAGAEIELVDIE
                     HRETRLKSALATLESEYDFILIDCPPALNLLTVNGLCAAHAVLIPMQCEYYALEGLSD
                     LVQTIKKVRTHLNPQLQIEGLLRTMFDPRNTLAQQVSAQLQQHFGDKLYRTVIPRNVR
                     LAEAPSHGVPVLGFDQKSKGAQAYLALAGEMLNRAAPVV"
     gene            961..>1659
                     /locus_tag="VHT22_00865"
     CDS             961..>1659
                     /locus_tag="VHT22_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX3136127.1"
                     /translation="MVMAKMKGLGRGLDALLGGEEPAVARAAGDQQAELKVDLLQPGK
                     YQPRTRMDEAALKELADSIRMQGVMQPILVRPVSGGRYEIIAGERRWRAARMAGLSDV
                     PALIREVPDNAALAMALIENIQREDLNPLEEANGIQRLVTEFNITHDTAAEMVGRSRS
                     AVSNLLRLLALSEPVRDLLHQGKLDMGHARALLALTGMQQIDTARLIAARGMSVRETE
                     KLVGRLLRGTPAKKP"
BASE COUNT          330 a          509 c          552 g          268 t
ORIGIN      
        1 gctggccggc ggcttgtgcg cgagcgacgg cgtgatcgcg gcgatgaagg gggtctatcc
       61 cgatgaagaa ctcgcgcaag tgcccgccac gttcaagcta atgagcgcag tgccgctcaa
      121 ggtgccggga ttgagcgccg agcgccatct cgttttgctc agccccgcaa gggcatgaga
      181 tggcgagaat actggcgatc acgaatcaaa aaggcggggt cggcaaaacg acgacgggtg
      241 tcaaccttgc cgcgagctta gcggcgacca aacagcgcgt cctgctgatc gacctcgatc
      301 cgcagggcaa tgcgacgatg ggcagcggcg tggataagcg cgcgctcaaa gtgacggtct
      361 atcacctgtt gctcggcgaa gcggaaatcg gcgcggtgcg cgtgcgctcg accagcggca
      421 actacgatgt gctgccggcc aatcgcgagc tcgccggcgc cgagatcgag ctggtcgaca
      481 tcgagcaccg cgaaacgcgg ctgaagtcgg cgctggccac gctcgaaagc gagtacgact
      541 tcatactgat cgattgcccg cccgcgttga acctgttgac ggtcaacgga ctgtgcgcgg
      601 cccacgcggt gctgatcccg atgcagtgcg agtattacgc gctcgagggg ctgagcgatc
      661 tcgtgcagac cataaaaaag gtgcgcaccc atctcaatcc gcagctgcag atcgagggcc
      721 tcttgcgcac gatgttcgat ccgcgcaata cgctcgcgca acaggtctcg gcccagctcc
      781 agcagcattt cggcgataag ctgtatcgca ccgtgatccc gcgcaatgtg cggctcgccg
      841 aggcgcccag tcatggcgtg ccggttttag ggttcgacca aaaatccaaa ggtgcgcaag
      901 cttacctggc gctcgccggc gaaatgctca atcgcgcggc gccggtggtt taaaaaggcg
      961 atggtgatgg cgaagatgaa gggactgggg cgcgggctgg acgcactact cggcggcgag
     1021 gagccggctg tggcacgggc ggcgggcgac cagcaggccg aactcaaagt cgatcttttg
     1081 cagcccggca aatatcagcc gcgcacgcgc atggacgaag cggccctgaa ggagttggcc
     1141 gattccatcc ggatgcaggg tgtcatgcag ccgatcctgg tgcggccggt cagtggcggc
     1201 cgttacgaaa tcatcgccgg cgagcgacgc tggcgtgccg cgcgcatggc cggtctgagc
     1261 gacgtgccgg cgctcatccg cgaagtcccc gacaacgccg cgctcgcgat ggccttgatc
     1321 gaaaacattc agcgtgaaga cttgaatccg ctcgaagagg cgaacggcat tcagcgcctg
     1381 gtcaccgagt tcaatatcac gcacgacacg gcggccgaaa tggtcggtcg ctcacgcagt
     1441 gccgtatcca acctgctgcg cctgctggcg ctctcggaac cggtgcgcga cctcctgcac
     1501 caaggcaagc tcgatatggg gcacgcgcgc gcgctgctcg cattgaccgg catgcagcaa
     1561 atcgacaccg cccgcttgat cgccgcgcgc ggcatgtcgg tgcgcgaaac tgaaaagctg
     1621 gttggccggc tgttgcgcgg aacgccggct aaaaagccg
//
LOCUS       DATANS010000060         3571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_591558, whole genome shotgun sequence.
ACCESSION   DATANS010000060 DATANS010000000
VERSION     DATANS010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591558"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..936
                     /locus_tag="VHT22_00870"
     CDS             43..936
                     /locus_tag="VHT22_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX3136128.1"
                     /translation="MAHTPEITNMALFCDFENIALGVQDAKYEKFDIGKVLERLLVKG
                     SIVVKKAYCDWDRYKAFKAAMHEAAFELIEIPHVRQSGKNSADIRMVVDALDLCYTKS
                     HVETFVIISGDSDFSPLVSKLRENNKVVIGVGVKNSTSDLLIANCDEFIFYDDLVREQ
                     KKAESRKAGARKAAGSGGAKKKGAEALSGHVEARDSSAPQAPRAEAKQADAGRQQEAI
                     DIIVSTVEALVSERGGDDKIWGSMVKQAVKRVRPGFNESYYGFRSFNGMLEAAEQAGS
                     LRLERDEKSGGYIVRLAAHEE"
     gene            942..1781
                     /locus_tag="VHT22_00875"
     CDS             942..1781
                     /locus_tag="VHT22_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012076218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX3136129.1"
                     /translation="MLRLLARLTGVLGLALGFSAVASAAEANLTVTVAGRTTTYAPAS
                     LLGMPAATSVTIPDDVAYKRDMSFRAVPAASLLEGVAPDDTLRFVASDGFVATLPAAL
                     LLARSGAIAYLAIEPVDAAWPPLKPEGGASAGPFYLVWLNPERARITPEQWPYQVARI
                     EAVAPLAKRYPMIAPAVSAHDTVRRGYAVFEKNCSVCHTLNLGGDAAVGPDLNIPYNP
                     TEYMRVDALRRLIRDPQSLRRWPAAKMPAFETRLLSDRDLTDLLAYLRHMADRKVTVP
                     SGK"
     gene            1851..2219
                     /locus_tag="VHT22_00880"
     CDS             1851..2219
                     /locus_tag="VHT22_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HEX3136130.1"
                     /translation="MPYRFAADAVLAAHLAFVLFVVFGGLLVLRIPRLAWLHLPAVAW
                     AALIEFSGWICPLTPLEIALRRSAGEAGYGGDFVEHYLVALIYPAGLTRELQVSIGAA
                     VLLINLVVYFVLWRRRSIRC"
     gene            complement(2190..3122)
                     /locus_tag="VHT22_00885"
     CDS             complement(2190..3122)
                     /locus_tag="VHT22_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003185974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX3136131.1"
                     /translation="MKRKSAPGRTAAAIRIGVSGWRYPPWRGVFYPKGLQQRRELEFA
                     SRVFPSIELNGSFYSLQRPENYASWYDETPSGFVFSVKAPRFITHIKRLRDIGAPLAN
                     FFASGVANLREKLGPILWQLPPTMRYDREVLEGFLASLPRDTAAASRLARRHDARVTG
                     RARIAFGPARRLRHALEVRHDSFIDPALVDQLRRHDVALVIADTAGKWPQVGDVTADF
                     VYIRLHGDEELYRSAYIEPVLTLWTERIRAWTDGGEPSGIKRISHEPAPAAGRREVFC
                     YFDNTDKVEAPANARRLMEKLNVEWSAPDRPAAP"
     gene            complement(3182..>3571)
                     /locus_tag="VHT22_00890"
     CDS             complement(3182..>3571)
                     /locus_tag="VHT22_00890"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX3136132.1"
                     /translation="NKADVKDGIMAYKIAAHAADLAKGHPGAQIRDNALSKARFEFRW
                     EDQFNLGLDPDKAREFHDETLPQHGAKLAHFCSMCGPHFCSMKITQDVRDYAAKLGVD
                     ESAALEKGMESKAIEFVKKGAEIYSKA"
BASE COUNT          604 a         1156 c         1168 g          643 t
ORIGIN      
        1 gaacggcctt tcgaatgagc cggctcacgt ccgggaaaat ccatggccca cacccccgaa
       61 atcaccaaca tggcgctgtt ctgcgatttc gagaacatcg cgctcggcgt ccaggacgct
      121 aagtacgaga agttcgacat cggcaaggtg ctggagcgct tgctcgtcaa aggcagtatc
      181 gtcgtcaaga aagcgtactg cgactgggac cgttacaaag cattcaaagc cgcgatgcac
      241 gaagcggcct tcgagctgat cgagattccg catgtgcgcc agtcgggcaa gaactcggcc
      301 gacatccgaa tggtcgtcga cgcgctcgac ctgtgctaca cgaagtcgca cgtcgagacc
      361 ttcgtgatca tcagcggcga ctccgatttc tcgccactgg tgtccaagct gcgcgagaac
      421 aacaaggtgg tgatcggcgt cggcgtcaag aattcgacct ccgacttgct gatcgcgaat
      481 tgcgacgagt tcatttttta cgacgactta gtgagggagc agaagaaggc ggagtcgcgc
      541 aaggccggcg ccaggaaagc agctgggagc ggaggcgcca agaagaaagg cgccgaagcg
      601 ctttccggcc acgtcgaagc cagggactcg agcgcgcctc aagcgccgcg ggcggaagcg
      661 aagcaggccg acgccggccg gcagcaggaa gcaatcgaca ttatcgtcag caccgtcgag
      721 gcactggtgt ccgagcgcgg cggcgacgac aagatctggg gatcgatggt caagcaggcg
      781 gtcaagcgcg tgcgtcccgg cttcaacgaa tcgtattacg gcttccgttc gttcaacggc
      841 atgctcgagg cggccgagca ggcgggtagc cttcgactcg agcgcgacga aaagtccggc
      901 ggctatatcg tgcgcctcgc cgcgcacgag gaatgagcgc gatgctgcgc cttcttgcga
      961 ggctgacagg cgtcctgggc ctcgcgcttg gtttttcggc ggtcgcgtca gccgccgagg
     1021 ccaatctcac ggttaccgtc gccgggcgga cgacgaccta cgctccggcg agcctgctcg
     1081 gcatgccggc ggcgaccagc gtcaccatac cggacgatgt cgcctacaag cgcgacatga
     1141 gcttccgcgc cgtgcccgca gcgagcctgc tcgaaggggt cgcgcccgac gacacgctgc
     1201 gctttgtcgc cagcgacggc tttgtcgcaa cgcttccggc ggcgctgctg ctggcgcgaa
     1261 gcggcgcgat cgcgtatctc gcgatcgagc cggtcgacgc ggcgtggcca ccgctcaagc
     1321 ccgagggcgg cgccagcgcg gggccgttct acctggtgtg gctcaatccc gagcgtgcgc
     1381 gcatcacgcc ggagcagtgg ccgtaccagg tcgcccgcat cgaggccgtc gcgccgctgg
     1441 ccaagcgcta tccgatgatt gccccggcgg tgtcggcgca cgacacggtg cggcgcggat
     1501 acgctgtgtt cgaaaagaac tgctcggtct gccacacgct gaacctcggc ggcgacgcgg
     1561 ccgtcggtcc ggatctgaat atcccataca accccaccga atacatgcgc gtcgatgcac
     1621 tgcggcggct gatccgcgat cctcagtcgc tgcgccgctg gcccgcggcc aagatgcctg
     1681 cctttgagac gcggttgttg agcgatcgcg atctgaccga tctcttggcc tacctgcggc
     1741 acatggccga tcgcaaggtc acggtgccct caggcaagta gcgcttcctc gtcggcgctg
     1801 cgccgcgcca ctctcggccc cgccgccgcg gaagcctccc ttccatgctc atgccgtacc
     1861 gcttcgccgc cgatgccgtc cttgcggcgc acctggcatt cgtactcttc gtggtattcg
     1921 gcggcctgct ggtgttgcgc ataccacgtc tcgcgtggct gcatcttccc gcggtggcgt
     1981 gggccgcatt gatcgaattc agcggatgga tctgcccgct gacgccgctg gaaatagcgc
     2041 tgcgccgcag cgccggcgaa gccggatacg gcggcgactt tgtcgagcat tatctggtgg
     2101 cgctgatcta tccggccggg ctgacgcgcg aactgcaggt ctcgatcggc gctgcggtgc
     2161 tgctgatcaa cctggtcgtc tactttgtct tatggcgccg ccggtcgatc cggtgctgac
     2221 cactccacgt tcagcttttc catcagccgg cgcgcgttcg ccggcgcttc gaccttgtcg
     2281 gtattgtcga aatagcaaaa gacctcgcgc cggccggccg cgggtgcggg ctcatgcgag
     2341 atacgcttga tgccgctcgg ctcgccgcca tcggtccagg cgcggatgcg ttcggtccac
     2401 agcgtcaaca ccggctcgat ataggcgctg cggtaaagct cttcatcgcc gtgcagacgg
     2461 atgtagacga aatccgcagt aacgtcgccg acctgcggcc atttgcccgc ggtgtcggcg
     2521 atgaccagcg cgacgtcatg ccggcgcaat tgatcgacga gcgccggatc gatgaagctg
     2581 tcgtggcgca cttccagcgc gtggcgcagc cggcgcgccg gcccgaaggc gattcgcgcg
     2641 cgtccggtga ccctggcgtc gtggcgccgg gctagccgcg aggcggcggc agtatcccgc
     2701 ggcagcgatg ccaggaaacc ctcgagcacc tcccggtcgt aacgcatcgt gggcggcagc
     2761 tgccagagga taggtccgag cttttcgcgc aggttggcga ctcccgaggc aaaaaaattg
     2821 gccagcggcg cgccgatgtc gcgcagccgc ttgatgtggg tgatgaagcg cggcgccttg
     2881 acgctgaaga caaagccgga tggcgtctcg tcgtaccagc ttgcataatt ttccggccgc
     2941 tgcagcgaat agaacgagcc gttgagctcg atcgagggaa acacacgcga agcgaattcc
     3001 agctcccgcc gctgttgcag ccccttcggg tagaacacgc cgcgccacgg cggatagcgc
     3061 cagccggaga cgcctatgcg gatggcggcg gccgtgcggc ccggcgcgct cttgcgtttc
     3121 atcctgttgt ccccgattcg gcggacgctg aggctcgacc ggagacctgg atgtgttccg
     3181 ctcaggcctt gctatagatt tcggcgccct ttttgacgaa ctctatcgcc ttcgattcca
     3241 tgcccttttc cagcgccgcc gactcgtcga cgccgagctt cgccgcgtag tcgcggacgt
     3301 cctgcgtgat cttcatcgag cagaagtgcg ggccgcacat cgagcagaaa tgcgccagct
     3361 tggcgccatg ctgcggcagg gtctcgtcgt ggaattcgcg cgccttgtcc ggatcgagcc
     3421 cgaggttgaa ctggtcctcc cagcggaact cgaagcgcgc cttggacaat gcgttgtcgc
     3481 ggatctgcgc gcctggatga cccttggcga ggtcggcggc gtgcgcggcg atcttgtagg
     3541 ccatgatgcc atccttgacg tcggccttgt t
//
LOCUS       DATANS010000061         1548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_213741, whole genome shotgun sequence.
ACCESSION   DATANS010000061 DATANS010000000
VERSION     DATANS010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213741"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..993)
                     /locus_tag="VHT22_00895"
     CDS             complement(157..993)
                     /locus_tag="VHT22_00895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3136133.1"
                     /translation="MSAVIPASPPFVDLAAVKARQQAAWSAGNYAVVGTTLQIVGEIL
                     CEALDLRAGSSVLDVASGNGNATLAAARRWCDVTSTDYVSSLLESGRARAQAEGHTIQ
                     FREADAENLPFPDKSFDAVLSTFGVMFTADQDRAAGELVRVCKPGGKIGLANWTPESF
                     IGQLFKTIANYVPPAAGVRSPALWGTNARLEELFGQAAQEIRITRRDFTFRYRSPSHW
                     IEVFRTYYGPMNRTFLALDAQRQAALTQDLLNLIDRRNRSRDGTLVLQSEYLEVVVQL
                     KR"
     gene            complement(1111..>1548)
                     /locus_tag="VHT22_00900"
     CDS             complement(1111..>1548)
                     /locus_tag="VHT22_00900"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEX3136134.1"
                     /translation="RNRYPGGDASREHPAIDEDDIEAWARHARGLNGLGGDAEAGAPA
                     ASALLSAYELHRAARIERAFTLAEIIAEMVWAVGAFARRAYARYRQRREASALRDTLR
                     QLDDRMLHDLGFDRSEISSVAAEFTREAESTRLRILLSHNPRW"
BASE COUNT          261 a          501 c          500 g          286 t
ORIGIN      
        1 ttcggttctc ctccagcgca cgtcgggcgg acattatcgc cgcacgggtc gcctttggga
       61 ataaccgtgg agtcgaggct cctgcgcttc gctccggtat tgcgcggatg cgaccgatct
      121 acagctgtgc acccgtcaac gcgccgtatc gggcaatcac cgcttcagct ggaccacgac
      181 ttcgaggtac tcgctttgca gcaccagcgt gccgtcccgc gaccgattgc ggcgatcgat
      241 gaggttcagc aggtcctggg tgagcgcagc ttgcctttgc gcatcgagcg cgaggaacgt
      301 cctgttcatc ggtccgtagt aagtgcgaaa cacctcgatc cagtgagacg gcgagcggta
      361 gcgaaacgta aaatcgcgcc gagtgatcct aatctcctgc gccgcctggc cgaaaagttc
      421 ctcgagtctt gcgttcgttc cccacaatgc cggcgatcgg acgccggcgg ctggagggac
      481 gtagttcgcg atcgtcttga acagctgacc gatgaagctt tccggcgtcc agttcgccaa
      541 gccgattttg ccgccgggct tgcagacgcg cacgagttcg cccgccgctc tgtcctggtc
      601 ggctgtgaac atcacgccga aggtggaaag cacggcgtcg aacgacttgt cgggaaaggg
      661 caggttctcc gcgtcggctt cccggaactg gatcgtgtgg ccctcggcct gcgcgcgagc
      721 ccggccggac tcgagcagcg aggacacata gtcggtcgag gtgacgtcgc accagcgccg
      781 ggcggctgcc agcgtggcgt tgccgttgcc ggaggcgacg tcgagcacgc tgctgcccgc
      841 gcgcaagtcg agcgcctcgc acaggatttc gcccacgatc tgcaaagtcg tgcctacgac
      901 ggcatagttt cccgcggacc aggcggcctg ctggcgcgcc ttgactgcgg cgagatcgac
      961 gaacggcggc gacgcgggaa tcactgcaga catcgcatta ctcctggctg ggtgaaaggg
     1021 gtaggccgtg gaacgcttcg gctgccccat gtgcctcagc gcgacgccga acatcgacgg
     1081 cgatttctgt tcgttctgat acatgagagg tcaccaccga gggttgtgcg acagaagaat
     1141 gcgcagtcgg gtgctctccg cctctcgcgt gaattccgcc gcgaccgagc ttatttcgct
     1201 gcggtcgaaa cccaggtcgt gcaacatgcg gtcatcgagc tggcgaagcg tgtcgcggag
     1261 cgcgcttgcc tcgcggcgct gtctatagcg cgcgtatgct cggcgtgcga atgcgccgac
     1321 tgcccacacc atttcggcaa tgatctcggc gagggtgaac gcccgctcga tccgcgccgc
     1381 acgatgcaat tcgtaagcgc tcaaaagagc ggacgcggcg ggcgcaccgg cctcggcatc
     1441 gccgccgagg ccgttcagtc cacgcgcatg acgcgcccac gcttcgatgt catcctcgtc
     1501 gatcgccgga tgctcgcgac tcgcgtcgcc gcccggatac ctgttccg
//
LOCUS       DATANS010000062         2899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_171839, whole genome shotgun sequence.
ACCESSION   DATANS010000062 DATANS010000000
VERSION     DATANS010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171839"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1124
                     /gene="galE"
                     /locus_tag="VHT22_00905"
     CDS             117..1124
                     /gene="galE"
                     /locus_tag="VHT22_00905"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX3136135.1"
                     /translation="MADTVLVTGGAGYIGSHIVVELARVGHQPIVVDNFCNSSPAVIP
                     RLSRITDRPIPSITADVRDKAALSRIFAEHSIAAVVHCAGLKAVGEGEAQPLSYYDNN
                     VGGAIALVAAMAAAGVRRLVFSSSAAVYGQPDKNPVAEDAPLRPHNVYGRTKRIVEQL
                     LEDLAAADRSWRIALLRYFNPAGAHSSAAIGEAPSGRPNNLVPLLAGVAAEKLAEIAI
                     YGDDWPTSDGTGVRDYIHVMDLAAGHVAALAYLASTPGAVALNLGTGRGHSVLELIAA
                     FERACGRTIPRRVAPRRPGDVAAYYADPARAEAVLGVRARRDIDAICADAWRWQQRSG
                     E"
     gene            1180..1485
                     /locus_tag="VHT22_00910"
     CDS             1180..1485
                     /locus_tag="VHT22_00910"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX3136136.1"
                     /translation="MERFYTALNRFDAYLLLHRLQHAGISAHVFNEHASSIVGEVPPD
                     IAQPQVWLANERDRPRAEAVLAALARERGQSGSVFCRSCHEENPANFELCWHCGASL"
     gene            complement(1530..2012)
                     /locus_tag="VHT22_00915"
     CDS             complement(1530..2012)
                     /locus_tag="VHT22_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEX3136137.1"
                     /translation="MARGAIGAWLMAIAITILAGAAPAADAPPVAPSAALEARLKKLE
                     TELRCLVCQNQTLADSNADLASDLRREVREQALAGKNDSEIRDYLVARYGDFVLYDPP
                     LKRTTWLLWFGPFALLAGGGLVWWQVLRRRQRASASAPTRDPAVEARARALLDDEPPA
                     "
     gene            complement(2012..2542)
                     /locus_tag="VHT22_00920"
     CDS             complement(2012..2542)
                     /locus_tag="VHT22_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEX3136138.1"
                     /translation="MKTLRWSIPLAVFVVLLAFLWVGLGRDPHEVPSPLIGKPAPAFQ
                     LVQLHDAGKTLAADDLKGKVWLLNVWASWCVSCRVEHPLLLQLAKTNVVPVYGLDYKD
                     KTADALAWLAQHGNPYTASIVDSDGKVGIDYGVYGVPETFLIDKAGVVRYKQIGPLTV
                     EALQQKILPLVRQLQQ"
     gene            complement(2554..>2899)
                     /locus_tag="VHT22_00925"
     CDS             complement(2554..>2899)
                     /locus_tag="VHT22_00925"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEX3136139.1"
                     /translation="ALVGTVEVRQDGRLIETLHPEKRIYNASGQTMTIAAIDTGISGD
                     RYVSLGEAIGGDGAIAGAWSVRIYLKPFVDWIWLGAFVMALGGFVAVSDRRYRLAVRR
                     RLGALAGAAAAE"
BASE COUNT          504 a          998 c          936 g          461 t
ORIGIN      
        1 catcgcgggc cactgccaag gctgccgcct gaacgacgcc gtcgagaaaa acgagccggg
       61 ctttcgcgcc cggctttttt tcgccccgcg tcaggcggct cgcgtaacat agcctgatgg
      121 cagacaccgt cctcgtcacc ggcggtgcgg gctatatcgg ttcgcacatc gtcgtcgagc
      181 ttgcccgcgt cggacatcag ccgatcgtcg tcgacaactt ctgcaacagc tcgccagcgg
      241 tgatcccgcg cctctctcgc atcaccgatc ggccgatacc gtccatcacg gccgacgtgc
      301 gcgacaaggc cgccctgtcg aggatctttg ccgagcactc gatcgcggcg gtcgtgcatt
      361 gcgcgggatt gaaggccgtc ggcgagggcg aagcgcagcc tctgagctac tacgacaaca
      421 acgttggcgg cgctatcgcg ctggtcgcgg cgatggcggc ggcaggtgtc aggcggctgg
      481 tgttcagttc gtcggcagcc gtctatgggc agccggacaa gaatcccgtt gccgaagacg
      541 cgccgcttcg cccgcacaac gtctacggtc gcaccaagcg catcgtcgag cagctgctcg
      601 aggacctcgc cgccgccgat cgctcatggc ggatcgcgct gctgcgctac ttcaatcccg
      661 ccggggcgca cagcagcgcg gcgatcggcg aggcgccatc gggcaggccg aacaacctcg
      721 tccctctcct ggctggcgtc gccgccgaaa agctcgccga aatcgccatt tacggcgacg
      781 actggccgac gtccgatggg accggcgtgc gcgactacat acacgtgatg gatctggccg
      841 cgggccacgt agctgctctc gcctatctgg cttcgacgcc gggtgccgtc gcgctcaatc
      901 ttggcaccgg acgcggccat tcggtactcg aattgatcgc cgcattcgaa cgtgcctgcg
      961 gtcggacgat accgcggcga gtcgcaccgc gcaggcccgg cgacgtcgcg gcttactatg
     1021 ccgatccggc gcgcgccgag gcggtgctgg gcgtgcgcgc gcgacgcgac atcgacgcca
     1081 tctgcgccga cgcgtggcga tggcagcagc gctccggcga gtgagcaccc gcacgccgag
     1141 ccatggtacc cgcggcatga tcacgcgtga aaacaagcga tggaacgctt ctacaccgcg
     1201 ctgaatcgat tcgacgccta cctgctgctg caccggctgc agcatgccgg tatcagtgcg
     1261 cacgtcttca acgagcacgc gtcgagcatc gtcggcgagg ttcccccaga catcgcccaa
     1321 ccgcaggttt ggctggcgaa cgaacgagac cgcccgcgcg cggaagccgt gctcgccgcg
     1381 ctggcgcgcg aacgcggtca atcgggcagc gtattctgcc gctcgtgcca cgaggaaaat
     1441 ccggcgaact tcgagctgtg ttggcactgc ggcgcgagtc tttagtatct gcgcctatgc
     1501 ggcaagaaca gccgcgctgg agtacgcgcc tacgccggcg gctcgtcgtc gagcaatgcg
     1561 cgcgcgcgcg cctcgacggc gggatcacgg gtcggtgccg aggccgacgc gcgctgccgc
     1621 cgccgcaaga cttgccacca caccaggccg ccgccggcga gcagggcaaa cgggccgaac
     1681 cacaacagcc aggtcgtgcg cttcaacggt gggtcataca gcacgaagtc gccgtagcgg
     1741 gcgacaagat agtcgcggat ttcggaatcg ttcttcccgg cgagtgcctg ctcgcgcact
     1801 tcgcggcgca agtcgctggc gagatcggcg ttcgaatcgg caagcgtctg attctggcaa
     1861 accaggcaac gaagctcggt ttcgagcttc ttgagccgcg cttcgagcgc cgctgacggc
     1921 gcgaccggcg gcgcatcggc cgccggcgca gcgccggcaa ggatcgtgat cgcgatcgcc
     1981 atcagccacg caccgatcgc tccgcgcgcc atcattgctg cagctgccgg accagcggaa
     2041 ggatcttctg ctgcagtgcc tcgacggtca gtggtccgat ctgcttgtag cggaccacgc
     2101 ctgccttgtc gatcaggaat gtctcgggca cgccgtacac gccgtagtcg atgccgacct
     2161 tgccgtcgct gtcgacgatc gacgcggtgt acgggttccc gtgctgggcg agccacgcga
     2221 gagcgtcagc cgtcttgtct ttgtagtcga gaccgtacac cgggacgacg ttggtcttgg
     2281 caagctgcag cagcagcgga tgctcgacgc ggcaggagac gcaccatgac gcccagacat
     2341 tgagcagcca taccttgccc ttgagatcgt cggccgcgag cgtttttccc gcatcgtgca
     2401 actgcaccag ctgaaatgcc ggggcgggct tgccgatcag cggggacggc acctcatgcg
     2461 gatcgcgtcc cagtccgacc cagagaaatg cgagcagcac gacgaaaacc gccagcggta
     2521 tcgaccaccg cagcgtcttc atcgacgata cggtcactcg gccgccgcgg cgccggccag
     2581 cgcgccaagc cgccgacgca ccgcgaggcg gtagcgccga tcggaaacgg cgacaaagcc
     2641 gccgagcgcc atgacaaacg caccaagcca gatccagtcg acgaagggtt tcaggtagat
     2701 gcgcacactc cacgcgccgg cgatcgcgcc gtcaccgcca atcgcctcgc caagcgagac
     2761 atagcgatcg ccggagattc ctgtatcgat cgccgcgatc gtcatcgtct ggccggacgc
     2821 gttgtagatg cgcttttccg gatgcagcgt ctcgatcagg cgcccgtcct ggcgaacctc
     2881 gaccgtgccg accagcgcc
//
LOCUS       DATANS010000063         1664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_549999, whole genome shotgun sequence.
ACCESSION   DATANS010000063 DATANS010000000
VERSION     DATANS010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549999"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VHT22_00930"
     CDS             complement(<1..283)
                     /locus_tag="VHT22_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEX3136140.1"
                     /translation="MTFVWPQMLWLLLLAPVLVGIYVWALSRRRKHALRYASVGLVRE
                     ALGRRQRFRRHIPPLLFLIALLATIFAIARPSAIVTLPSDQRTTILAIDV"
     gene            complement(375..>1664)
                     /locus_tag="VHT22_00935"
     CDS             complement(375..>1664)
                     /locus_tag="VHT22_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX3136141.1"
                     /translation="PEQILPAVKDQYERYPYPLREAAEEKTRIIRTWLDDLPMINQYC
                     FGGRRRFDRGFRVLVAGAGTGDGTIYLAEQLRETDAEIVHVDISARSNEIARERAAVR
                     QLRNIRWINGSLLSLSRGDLGVFDYINCCGVLHHLEDPEAGMSALLPLRADGGAFGIM
                     VYAQYGRTGIYQMQSLLRLINAGLDDPERKLANAKALLPCLPRTNWFARAQDLYREHV
                     IGGDAGMYDMLLHSQDRAYTVAQIYAWFEDKCGLSIQFTDVNRGPAAYTPRLLAPPGE
                     LALWRDVDALPLRRQQEIAELLSGSIKMHVFYATAGSGTKAPYGDPDYVPFFFHEPVT
                     GAELYDLITRHHDRPFVLNHSFSGITTRVDPGRFAKYVLRHIDGKRTFRQIFERVRAE
                     PACAAAAPGDEELFADFAGFFGVLHAIDRLLLRHHTA"
BASE COUNT          295 a          506 c          573 g          290 t
ORIGIN      
        1 agacgtcgat cgccaatatg gtcgttcgct ggtcggacgg cagcgtgacg atggcgctgg
       61 gccgtgcaat ggcgaagatt gtcgcgagca gcgcgatcag gaaaagcagg ggcgggatat
      121 ggcggcgaaa gcgctgccga cggccgagcg cttcccggac caggccgacg cttgcgtagc
      181 gcaaggcgtg cttgcgcctc ctggaaagcg cccagacata gatgccaacc agcaccggcg
      241 ccagcaatag cagccacagc atctgcggcc agacgaaagt catcttactt gcccgggttt
      301 gttgcggcgg ctgcgcatga tcgtatttcg gactccgcgc cggcgaatgc gccacgcgga
      361 ttgcagcgac aagtctatgc cgtgtgatgg cgcaaaagca accggtcgat ggcgtgcaga
      421 accccgaaaa atccggcaaa gtcggcaaac agctcctcgt cgccgggcgc ggctgcggcg
      481 catgccggct ccgcgcgcac ccgctcgaag atctgccgga acgtgcgctt cccgtcgatg
      541 tgcctgagta cgtatttcgc gaagcgcccc ggatcgacgc gcgtcgttat gccgctgaag
      601 ctgtggttga gaacgaacgg gcggtcgtgg tggcgggtga tcaagtcgta cagctcggcg
      661 cccgtcaccg gctcgtgaaa gaagaatggc acgtagtcgg gatctccgta gggtgctttg
      721 gttccgctgc cggcagtcgc gtagaacacg tgcatcttga tgcttccgct cagtagctcg
      781 gcgatctcct gttgccggcg cagcggcagc gcatcgacgt cgcgccagag cgcgagctcg
      841 ccgggcggcg cgagcagccg cggcgtgtac gccgcgggtc cccggttgac gtcggtgaac
      901 tggatcgaaa gtccgcactt gtcctcgaac caggcgtaga tctgtgccac cgtgtaggcc
      961 cgatcctgcg aatgcagcag catgtcgtac atgccggcat cgccgccgat cacgtgctca
     1021 cgatacagat cctgggcgcg cgcgaaccag ttggtgcgcg gcaggcacgg cagcagcgcc
     1081 ttcgcgttgg cgagcttgcg ttcgggatcg tcgaggccgg cattgatcag gcgcagcagc
     1141 gattgcatct ggtagatgcc ggtgcgcccg tattgggcgt agaccatgat cccgaaggcc
     1201 ccgccgtcgg cgcgcagcgg cagcagcgcg gacatgccgg cttccgggtc ctcgaggtga
     1261 tgcagcacgc cgcagcagtt gatgtagtcg aagacgccga gatcgccgcg ggagagcgaa
     1321 agcagcgagc cgttgatcca gcggatgttg cgcagctggc ggacagcagc ccgttcccgc
     1381 gcgatttcgt tgctccgggc gctgatgtcg acatggacga tttcggcatc ggtctcgcgc
     1441 agttgctcgg ccaggtagat cgttccgtcg ccggtgccgg cacccgcaac gaggacgcgg
     1501 aatccgcggt cgaaacggcg ccggccgccg aaacagtact ggttgatcat aggcagatcg
     1561 tccagccagg tgcggatgat tcgcgtcttc tcttccgcgg cttcgcgcag cggataggga
     1621 tagcgctcgt attgatcctt gacggcaggc aggatctgtt cggg
//
LOCUS       DATANS010000064         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_550006, whole genome shotgun sequence.
ACCESSION   DATANS010000064 DATANS010000000
VERSION     DATANS010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550006"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VHT22_00940"
     CDS             <1..348
                     /locus_tag="VHT22_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX3136142.1"
                     /translation="CDIHPLNNLRVLRYLVHTLKIGEEAKDAWYRHWIDTGLAALEAQ
                     LAADAATGTFCHGDVPTMADVCLVPQLANARRANIPLDGYPTLLRIDATCRALDAFAR
                     AAPDRQPDADSSA"
     gene            390..1088
                     /locus_tag="VHT22_00945"
     CDS             390..1088
                     /locus_tag="VHT22_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX3136143.1"
                     /translation="MKYAIPSWDIPSVPVAGTDFAFPVRHIYCVGRNYAEHAKEMGGD
                     AAKEPPFFFTKPADAIVPVVPPAVADVQYPMATKNYHHEIELVVAIGGRGAGIAPERA
                     EALIYGYAVGLDMTRRDLQSAMREIKRPWDIGKSFAQAAPIGPIHRVAEVGHPKRGRI
                     GLEVNGTIRQQGDLSDMIWDVPHMLHFLSQYYELLPGDLVFTGTPAGVAAVVAGDMLI
                     GRIESLGELRIRIV"
     gene            1091..>1544
                     /locus_tag="VHT22_00950"
     CDS             1091..>1544
                     /locus_tag="VHT22_00950"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3136144.1"
                     /translation="MLSADFVEREYNLRQAFPDHPQWFARWSSESAAARSCLDSRLDI
                     RYGSGPKQTLDLFPAKSPRGALLFIHGGYWRALDKDDHSFVAPAFVDQDVGVAIINYD
                     LCPNVSIAHIVEECRQAVAWLWREGETHGVPVDRLFIAGHSAGGHLVAM"
BASE COUNT          268 a          514 c          485 g          277 t
ORIGIN      
        1 tgcgacatcc atccgttgaa caatctgcgc gtgctgcgct atctggtgca cacgctcaag
       61 atcggcgagg aggccaagga tgcctggtat cggcactgga tcgacacggg ccttgcggcg
      121 ctcgaagcgc agctcgcggc agatgccgcg acgggaacgt tctgtcacgg cgatgtgccg
      181 acgatggccg acgtctgcct ggtaccgcag ctcgccaatg cgcggcgcgc caacattccg
      241 ctcgacggct atccgacgct gttgcgcatc gatgcaactt gccgggcgct ggatgccttc
      301 gcccgcgctg cgcccgatcg ccagccagac gccgattcaa gcgcgtgagg ttctgcggtc
      361 atcgattctc ttctgcaaat aggccgacga tgaaatacgc gattccctcc tgggacatcc
      421 cttcggtgcc ggtcgccggc accgatttcg cctttccggt ccgccatatc tattgcgtag
      481 gtcgcaacta cgccgagcac gccaaggaga tgggcggaga cgcggcgaaa gagccgccgt
      541 tctttttcac caagccggcc gatgcgatcg tcccggtggt gccgcccgcg gtcgccgacg
      601 tgcagtaccc gatggcaacc aagaattatc atcacgaaat cgagctggtg gtcgcgatcg
      661 gcggacgcgg cgccggcatc gcgccggagc gcgccgaggc gctgatctat ggctacgcgg
      721 tcgggctcga tatgacgcga cgcgatctgc aaagcgcgat gcgggaaatc aagcgtccgt
      781 gggacatcgg caagtcgttc gcgcaggcgg cgccgatcgg cccgatccac cgcgtcgccg
      841 aggtcggaca tccgaagcgc ggccgcatcg gcctcgaggt caacggcacg atccgccagc
      901 agggcgatct ttccgacatg atctgggacg tgccgcatat gctgcacttc ctgtcgcaat
      961 actacgaatt gcttcccggt gatctggtct tcaccggcac gcccgcgggg gtcgccgccg
     1021 tcgtggccgg cgacatgctg atcggcagga tcgaaagcct gggcgagctg cgcatacgca
     1081 ttgtttgatc atgctctcag ccgacttcgt cgagcgcgaa tacaatttgc gccaagcctt
     1141 tcccgaccat ccgcaatggt tcgcgcgatg gtcctcggaa agcgccgccg cccgatcctg
     1201 cctcgattcg cgactggaca ttcgctacgg cagcggaccg aagcagacgc tcgacctgtt
     1261 cccagccaaa tcgcctcgcg gcgcgctgct gttcatccac ggcggatact ggcgcgcgct
     1321 cgacaaggac gaccattcgt tcgtcgcgcc ggcgttcgtc gaccaggatg tcggcgtcgc
     1381 catcatcaat tacgacctgt gccccaacgt cagcatcgcg cacatcgtcg aggaatgccg
     1441 gcaggcggtc gcgtggctat ggcgcgaagg cgaaacccat ggcgtgccgg tcgaccggct
     1501 gttcatcgcc ggacactccg ccggcggcca tctggtcgcg atgc
//
LOCUS       DATANS010000065         2379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_508148, whole genome shotgun sequence.
ACCESSION   DATANS010000065 DATANS010000000
VERSION     DATANS010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508148"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1151)
                     /gene="rnd"
                     /locus_tag="VHT22_00955"
     CDS             complement(3..1151)
                     /gene="rnd"
                     /locus_tag="VHT22_00955"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX3136145.1"
                     /translation="MDLISTTDDLAAVCGRMAKHPFVTVDTEFLRETTYYPLLCVAQM
                     ASPEEAVVVDALATGLDLSPLFALMASESVTKVFHAARQDIEIVWNMAKTIPHPIVDT
                     QVAAMVLGYGDSISYDQLVQRITGDTLDKSHRFTDWTRRPLSDAQIAYALSDVTHLRD
                     VYLKLATDLEKRGRSNWVEAEMEVLTSPETYRADPDRAWERLKSRVRKPKELAVLMEV
                     AAWREREAQTRDVPRGRVIKDDVIGDIAVQAPTSIERLGHLRSLPKGFERSRWGEQII
                     EAVKRGIARDPKTLPRLDRFRPAANGTATVELLKVLLRMTAERHGVAAKVIATVDDLD
                     RIAADDSADVPALKGWRRDLFGEKALALKQGRLALAVDNGKVVTVDKN"
     gene            complement(1212..>2379)
                     /locus_tag="VHT22_00960"
     CDS             complement(1212..>2379)
                     /locus_tag="VHT22_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX3136146.1"
                     /translation="RGRPGPAALECAMDIWGKRGPVAAIAAPSPPRTAKIDDSKIRAA
                     AKILGQAKRVLIVAGGGAQDASTEVTLLSEMLQAPVLGYRRGRGVLDSRNPFSVTLPL
                     GRELWGEADVVLAVGTRLLNPMRQWGVDKKLQIVRVDADPDEPARLHKPKVALVGDAA
                     PILRRLIDSLAKFNTRRASRADEMRQRQAKLQERLAKLAPQLAFLEAIRAELPEDGIY
                     VDEVTQMGFVARLALPVYGPRTFLSPGYQDNLGWGYATALGVQHAKPDVPVLSINGDG
                     GFLYTGNEIATAIRHRIPLVAVVFVDGAFGNVRRIQQEQFGNRLIASDLANPDFVKYA
                     ESFGAAGRRARGPDELRVALRESFARREPTLIEVPVGSLPSPWEFIHMPRVRGK"
BASE COUNT          410 a          791 c          759 g          419 t
ORIGIN      
        1 atctaattct tgtcgacggt tacaactttt ccgttgtcga cggcgagcgc gagccgacct
       61 tgctttagcg cgagcgcttt ctcgccaaac aggtcgcggc gccagccctt gagggcgggg
      121 acgtcggcgg agtcgtcggc ggcgatccgg tcgaggtcgt cgacggtggc gatcactttg
      181 gcggcgaccc cgtgccgctc cgccgtcatg cgcaggagta ccttgagcag ttcgaccgtc
      241 gccgtgccgt tggccgccgg acggaagcga tcgagccgcg gcagcgtctt cgggtcgcgc
      301 gcgatcccgc gtttgaccgc ctcgatgatc tgctcgcccc agcgcgagcg ctcgaagccc
      361 ttcggcagcg agcgcaggtg acccagccgc tcgatcgagg tcggcgcctg cacggcgatg
      421 tccccgatca cgtcgtcctt gatcacgcgc ccgcgcggaa catcgcgcgt ctgcgcctcg
      481 cgctcgcgcc aggcggcgac ctccatcagc acggcgagct cttttggctt gcgcacccgg
      541 ctcttgagcc gctcccaggc ccggtccgga tcggcgcgat aagtttcggg cgaggtgagc
      601 acttccatct ccgcctcgac ccagttgctg cgcccgcgtt tctcgagatc ggtcgcaagc
      661 ttcaggtaga cgtcgcgcaa atgcgtcacg tcggacaggg cgtaggcgat ctgtgcatcg
      721 ctgagcggcc gccgcgtcca gtcggtgaag cggtgcgatt tgtcgagcgt atcgccggtg
      781 atgcgctgca caagctggtc gtacgagatc gaatcgccat agccgagaac catggcggcg
      841 acctgggtat cgacgatcgg atgcgggatc gtcttggcca tgttccagac gatctcgata
      901 tcctgccgcg cggcatggaa caccttggtg acgctttccg atgccatcag cgcaaacaaa
      961 ggcgatagat cgagcccggt cgccagcgcg tcgacgacga ccgcctcttc aggggaggcc
     1021 atctgtgcga cgcacaacag cgggtaatag gtggtctcgc gcaggaattc ggtatcgacc
     1081 gtgacgaacg gatgcttcgc catccgcccg caaaccgccg cgaggtcatc cgtcgtgctg
     1141 attaaatcca tcgtgccgcg cacctaaccc gatccgacgc cgtcatggca actagacaat
     1201 ggcaactcaa ttcactttcc acgcacgcgc ggcatatgga tgaattccca ggggctgggc
     1261 agcgacccga ccggtacctc gatcaaagtc ggttcgcggc gggcgaagga ttcccgcaga
     1321 gccacacgga gctcatccgg acctcgggcg cgacgtccgg cggcaccgaa gctctccgcg
     1381 tatttcacaa aatcgggatt ggcaaggtcg gaggcaatca gccgattacc gaattgctcc
     1441 tgctggatac ggcgcacatt gccgaaagcc ccgtcgacga acaccaccgc caccagtggt
     1501 atccggtgac gaatggcggt ggcgatttcg ttgccggtat aaagaaatcc gccatcgcca
     1561 ttgatcgaga gcaccggcac gtccggcttt gcatgctgca cgccaagcgc cgtggcgtag
     1621 ccccagccga gattgtcctg gtacccgggc gacaggaacg tgcgtgggcc gtagaccggc
     1681 aaggcgagcc gcgcgacaaa tcccatctgg gtgacttcat cgacatagat gccgtcctcc
     1741 ggcaactcgg cgcggatcgc ctcgaggaag gcgagttggg gcgcgagctt ggccagccgc
     1801 tcctgcagct ttgcctggcg ctggcgcatt tcgtccgcgc gcgatgcgcg gcgcgtattg
     1861 aatttggcga gactgtcgat cagccgcctc aggatcggcg cggcgtcgcc aacgagcgca
     1921 accttgggct tgtgcagccg cgccggttca tcggggtcgg cgtcgacgcg cacgatctgc
     1981 agtttcttgt ccacgcccca ttgccgcatc gggttgagca gccgcgtgcc aacggccaaa
     2041 accacgtcag cctcacccca taattcgcgg ccgagcggca acgtcacgct gaacggattg
     2101 cgcgaatcga gcaccccgcg gccgcgccga tagccgagca ccggcgcctg cagcatttcg
     2161 gacaggagcg tgacctcggt cgaggcatcc tgcgcaccgc caccggcaac aatcagcacg
     2221 cgcttcgcct ggccgagaat tttcgcagcc gcgcgaatct tgctgtcatc gattttcgcg
     2281 gtgcgcggcg gagagggcgc cgcgatcgcg gcaacgggcc cgcgtttgcc ccagatgtcc
     2341 atcgcgcatt ccagtgctgc cggtcccggg cgtccgcgg
//
LOCUS       DATANS010000066         5505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_465888, whole genome shotgun sequence.
ACCESSION   DATANS010000066 DATANS010000000
VERSION     DATANS010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465888"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..677)
                     /locus_tag="VHT22_00965"
     CDS             complement(69..677)
                     /locus_tag="VHT22_00965"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX3136147.1"
                     /translation="MNIQPVVFYVFAAVLVFAALRVISSSNPVHSALWLVLSFFSAAG
                     IWLLLQAEFLAIALVLVYVGAVMVLFLFVVMMLDVNFAKMREQFKSYIPVGATVGILI
                     LVEMTLVLLGGYFAPATDAAPAAAAAGYSNTRALGLLLYTDYAYPFEIAAVILLVAII
                     AAIALTHRAPRTSKRQDPAAQVKVRRGDRLRVLDMPAEKNEG"
     gene            complement(681..1169)
                     /gene="nuoI"
                     /locus_tag="VHT22_00970"
     CDS             complement(681..1169)
                     /gene="nuoI"
                     /locus_tag="VHT22_00970"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX3136148.1"
                     /translation="MQRVRDFFNAYLLIELWQGLRVTLTNMFRPKITVQFPEEKTPMS
                     PRFRGLHALRRYPNGEERCIACKLCEAVCPALAITIESEAREDGTRRTTRYDIDLTKC
                     IFCGFCEESCPVDSIVETRILEYHGETRGDLIYTKEMLLAVGDRYEAQIAADRAQDAK
                     YR"
     gene            complement(1182..2234)
                     /gene="nuoH"
                     /locus_tag="VHT22_00975"
     CDS             complement(1182..2234)
                     /gene="nuoH"
                     /locus_tag="VHT22_00975"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX3136149.1"
                     /translation="MLSPVQDFFGPTWPAVWTLAKIVAIAIPLILAVAYLTLAERKVI
                     GWMQVRIGPNRVGPLGLLQPFADVFKLVFKEIIVPSGASRSLFFLAPLLSLGPALAAW
                     AVIPFDDGMVLSNINAGLLYILAMSSIGVYGVILAGWASNSKYAFLGCLRSAAQIVSY
                     EIAMGFALVGVLMAASSLNISDIVRGQSGGFWHWYLLPLLPLFVVYLVSGIAETNRHP
                     FDVAEGESEIVAGFHVEYSGVTFAVFFLAEYMNMILISTLASTMFLGGWLAPLPILNE
                     IGIAGLHPLGDGFLWLFVKICAMLFIFLWIRATFPRYRYDQIMRLGWKIFIPVTLVWI
                     AFLGAMMQTRWAYLFH"
     gene            complement(2240..4570)
                     /gene="nuoG"
                     /locus_tag="VHT22_00980"
     CDS             complement(2240..4570)
                     /gene="nuoG"
                     /locus_tag="VHT22_00980"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEX3136150.1"
                     /translation="MLTLEVDGKSVQVPNGSTVMDAAHKLDIYVPHFCWHKKLTIAAN
                     CRMCLVQVEKAPKPQPACATPAAEGMKVWTHSEAAVTAQKGVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGASSSRYDEPKRVVLNKNLGPLIATDMTRCIHCTRCVRFGQEIA
                     GVMELGMTNRGEFAEIMTFVGRTVDSELSGNMIDLCPVGALTSKPFRYTARTWELARR
                     KSVSPHDGLGSNLIVQVKNDRVMRVVPLENEPINECWLSDKDRFSYEGLNSQDRLTRP
                     LLRRDGRLVESDWQTALEYAAAELVRVRDQYGADAVGALVSPHSTLEELHLAQKLIRG
                     LGSDNIDFRLRQSDFSVDTPPAGAPWLGMSIAEFAALDRILIVGSFLRNDHPLLAHRL
                     RQAAKKGAQLSLVHASDDDLSIRVARKAIVAPSAMPRVLAEIVVAAAAGAGKPAPAAL
                     SGITASADAQAIAASLASGERKGIFLGNFAQHHPNAAQLHALAQELARLTGATQGFLT
                     EAANSVGGHIAGAMPQRGGMNANAMLVSPRKAYLLLHTETDLDAADPGVARAALKAAE
                     CVIALSPFRSGVAEHADVVLPISPFSETAGTFVNCEGRVQAFNGVAPPLGEARPAWKV
                     LRVLGSLLGLAGFAQDSIEAVRLELPAAEAIAAGLSNATGTAIGAPASTDAVPERVAD
                     VPMYFADPLVRRAASLQKTRNARPPAVRASAATLARLQLADGDAARVRRGEGEAMLKV
                     VADPSVPDGCVRIAAAHPTTSMLGPMFGSIVVEAVL"
     gene            complement(4604..>5505)
                     /locus_tag="VHT22_00985"
     CDS             complement(4604..>5505)
                     /locus_tag="VHT22_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX3136151.1"
                     /translation="EIFEVYERFEEALEEARAAGYLGDNLFGSGYSFQLHAAHGFGAY
                     ICGEETALLESLEGKKGQPRYKPPFPASFGLYGKPTTINNTETFAAVPWIINNGGEAF
                     LALGRQNNGGTKIFSVSGDVARPGNYEIKLGTPFAQLLDMAGGMRAGRKLKACIPGGS
                     SMPVLPGEVMLATDMDYDSIAKAGSMLGSGAVILMDDTRCMVRSLLRLSYFYFEESCG
                     QCTPCREGTGWLWRVVDRIEHGKGKNEDLDLLLSVSDNIAGRTICALGDAAALPVKSF
                     IKHFRAEFQHHIDHKQCLVPAYV"
BASE COUNT         1005 a         1810 c         1787 g          903 t
ORIGIN      
        1 ctccctgaag gctgcggccg cgtccgcggc cttgccattg ttgcttctta cagtgatctt
       61 cgccgcgctc agccctcgtt cttctcggcc ggcatgtcga gcacgcgcag acgatcgcca
      121 cggcgcacct tgacctgggc cgccggatcc tggcgcttac tcgtgcgcgg tgcgcgatgc
      181 gtcagcgcga tggccgcgat gatcgccacc agcaggatca ccgccgcgat ttcgaacgga
      241 taggcgtaat cggtatagag gagcaggccc agagcgcggg tgttgctgta acccgcggcg
      301 gcagccgccg gcgccgcgtc ggtcgctggg gcgaagtagc cgccgagcag caccaaggtc
      361 atctcgacca ggatcagtat gccgaccgtc gcgccgaccg gaatgtagct tttaaattgc
      421 tcgcgcatct tggcgaagtt gacgtcgagc atcatcacga caaaaaggaa aaggaccatc
      481 accgcgccga cgtagaccag cacaagagcg atggcgagga actcggcctg cagcagcagc
      541 catattcccg cggcactgaa gaacgaaagc accagccaca gcgccgagtg cacggggttg
      601 ctgctgctga tcacgcgcaa ggcggcgaag accagcactg ccgcgaaaac gtaaaagacg
      661 accggctgga tattcacggc ttatctatac ttcgcatctt gagcgcgatc ggccgctatc
      721 tgcgcctcgt agcggtcgcc gacggcgagc agcatttcct tcgtgtagat caggtcgccc
      781 cgcgtttcgc cgtggtactc gagtatccgc gtttcgacga tcgagtcgac cgggcacgac
      841 tcctcgcaga acccgcagaa tatgcatttg gtgagatcga tgtcgtagcg cgtcgtgcgc
      901 cgtgtgccgt cctcgcgcgc ttctgactcg atcgtgatcg ccagcgccgg gcacaccgct
      961 tcgcacagct tgcacgcgat gcagcgctcc tcgccgttcg gatagcggcg cagcgcgtgc
     1021 agcccgcgaa agcgcgggct catcggcgtc ttttcctcgg ggaactggac ggtgatcttc
     1081 ggccggaaca tgttcgtcaa agtgacgcgc aggccctgcc acagctcgat cagcaggtag
     1141 gcgttgaaga agtcgcggac tctctgcagc atcgccaatg ctcagtggaa aaggtaggcc
     1201 cagcgcgtct gcatcatcgc gccgagaaaa gcgatccaca ccagcgttac cgggatgaat
     1261 attttccagc ccagccgcat gatctggtcg taacggtagc ggggaaaggt cgcgcgtatc
     1321 cacagaaaga tgaacagcat cgcgcagatc ttgacgaaca gccagaggaa accatcgccg
     1381 agcggatgca gcccggcgat cccgatctcg ttcaggatcg gcagcggcgc cagccagccg
     1441 ccgaggaaca tggtcgaggc cagcgtggag atcaggatca tgttcatgta ttcggcgagg
     1501 aagaacaccg cgaacgtcac tcccgagtac tcgacgtgga agccggcaac gatctcggac
     1561 tcgccttcgg cgacgtcgaa cgggtggcga ttcgtctcgg cgatgcccga taccaggtag
     1621 acgacgaaca gcggcagcag cggcagcagg taccagtgcc agaagccgcc gctctggccg
     1681 cgcacgatgt cgctgatatt gaggctcgat gccgccatca gcacgccgac cagcgcaaag
     1741 cccatcgcga tctcgtacga cacgatctgc gccgccgagc gcaggcagcc gaggaacgca
     1801 tatttggaat tcgaggccca gccggcgagg atgactccat agacgccgat cgagctcatc
     1861 gccagaatgt agagcagccc ggcgttgatg ttggacagga ccatgccatc gtcgaacggg
     1921 atcacggccc aggcggctaa cgccgggccg agcgagagca gcggggccag gaagaagaga
     1981 ctgcggctcg ctcccgacgg aacgatgatc tccttgaaca ccagcttgaa aacgtcggcg
     2041 aacggctgca gaaggcccag cggaccgacg cggttggggc cgatgcgaac ttgcatccag
     2101 ccgatcacct tgcgctcggc cagcgtcaga taggcgacgg cgaggataag cggaatcgcg
     2161 atggcgacga tcttggccag cgtccacaca gccggccagg tcggaccgaa aaaatcctgg
     2221 accggagaga gcagctcgtt cacaggaccg cctcgaccac gatcgacccg aacatcggcc
     2281 ccagcatgct cgtcgtcgga tgcgcggccg caatacgcac gcagccgtcg ggcacgctgg
     2341 gatcggctac gaccttgagc atcgcctcgc cctcgccgcg gcgcacgcgg gcagcgtcac
     2401 cgtcggcaag ctgcagccgc gccaaggtcg ccgcgctggc gcgcacggct ggcggccgcg
     2461 cgttgcgcgt cttctgcagc gaagccgcgc gccgcaccag cggatcggcg aaatacatcg
     2521 gcacgtcggc gacgcgctcc ggcacggcgt cggtactggc cggtgcaccg atcgcggtgc
     2581 cggtcgcatt cgacagaccc gccgcgatcg cctccgccgc cggcaattcc agacggacag
     2641 cctcgatgct gtcctgcgcg aagcctgcca ggccgagcag cgaccccagc acgcgcagca
     2701 ctttccaggc cggcctcgcc tcgcccagcg gcggcgcgac gccgttgaac gcctgcacgc
     2761 gcccctcgca gttgacgaag gtgccggcgg tctcgctgaa gggtgagatc ggcagcacga
     2821 catcggcgtg ctcggccaca ccggaacgaa acggcgagag cgcgatcacg cattcggcgg
     2881 ccttcagcgc cgcgcgcgcg acacccgggt ccgcggcgtc aagatcggtc tccgtatgca
     2941 aaagcaggta ggctttgcgt ggcgagacca gcatcgcatt cgcattcatg ccgccgcgtt
     3001 gcggcatcgc ccctgcaatg tggccgccca ccgaattggc cgcttcggtc aaaaagcctt
     3061 gtgttgcgcc agtcagccgc gcgagctcct gcgcgagcgc atgcaactgg gccgcgttcg
     3121 gatggtgttg cgcgaagttg cccagaaaga tgcccttgcg ctcgcccgag gcgaggcttg
     3181 ccgcgatggc ctgcgcgtcc gccgacgcgg taatgccgga aagagccgcc ggcgccggtt
     3241 tgcccgcgcc cgcggccgct gcgacgacga tttccgccag cacgcgcggc atcgccgacg
     3301 gcgcgacgat cgccttgcgc gcgaccctga tcgatagatc gtcgtcgctc gcgtgcacca
     3361 gcgaaagctg cgcacctttt ttggccgcct ggcgcagccg gtgcgcgagc agcggatggt
     3421 cgttgcgcag gaagctgccg acgatcagta tgcgatcgag cgcggcgaac tcagcgatgg
     3481 acatgccgag ccagggagca cccgccggtg gcgtatcgac ggagaaatcc gattgccgca
     3541 gcctgaaatc gatgttgtcg ctgccgagac cgcgaatgag cttttgcgcg agatgcaatt
     3601 cctcgagcgt cgaatgcggc gacaccagcg cgccgacggc atccgcgcca tattggtcgc
     3661 gcacgcggac gagctcggcg gcggcgtact cgagcgccgt ttgccagtcg gattcgacca
     3721 gccgtccgtc acgccgcagc aacggacgcg tcagccggtc ctgcgaattg agcccctcgt
     3781 aggaaaaccg gtccttgtcc gacagccagc attcgttgat cggctcgttt tccagcggca
     3841 cgacgcgcat gactctgtcg ttcttgacct ggacgatgag attgctgccc aacccgtcgt
     3901 gcggcgacac cgattttcgt cgcgccagct cccaggtccg cgccgtgtag cggaacggct
     3961 tcgacgtcag cgcgccgacc ggacacaggt cgatcatgtt gccggacagc tcggagtcaa
     4021 cggtgcggcc gacgaaggtc atgatctccg cgaactcgcc gcggttcgtc atgcccagct
     4081 ccatgacgcc tgcgatctcc tggccgaagc gaacgcagcg cgtgcaatgg atgcagcgcg
     4141 tcatgtcggt cgcgatcagc gggcccaggt tcttgttgag caccacccgc ttgggctcgt
     4201 cgtagcgcga gctcgacgcg ccgtagccga ccgcgagatc ctgcagctgg cattcgccac
     4261 cctggtcgca gatcgggcag tccagcgggt gattgatcaa caggaattcc atcacgccct
     4321 tttgcgcggt gacggcggct tcggagtggg tccagacctt catgccttcg gcggccggtg
     4381 tcgcgcaggc cggctgcggc ttcggcgcct tctcgacctg gaccaggcac atgcggcaat
     4441 tggcggcgat cgtgagcttc ttgtgccagc agaaatgcgg gacgtagatg tcgagcttgt
     4501 gcgcggcgtc catcaccgtg ctgccgttgg gcacctggac ggacttgccg tcgacttcga
     4561 gcgtcagcat cgcgcgcacc cggaagctgg cgcggtcgga aggtcaaaca tacgcgggca
     4621 ccaggcactg cttgtggtcg atgtggtgct ggaattcggc gcggaaatgc ttgatgaagc
     4681 tctttaccgg cagcgccgcg gcgtcgccca gcgcgcagat cgtgcgtccc gcgatgttgt
     4741 ctgacaccga cagcagcagg tcgaggtcct cgttttttcc cttgccgtgc tcgatgcggt
     4801 cgacgacgcg ccacagccag ccggtgccct cgcggcacgg cgtacactgc ccacaggact
     4861 cctcgaagta gaagtaggac agccgcaaca gcgaccgcac catgcagcgc gtgtcgtcca
     4921 tcaggatcac cgcgccggag cccagcatcg agccggcctt ggcgatcgag tcgtaatcca
     4981 tatcggtggc aagcatgacc tcgccgggca gcacaggcat cgacgagccg ccggggatgc
     5041 acgccttgag cttgcgcccc gcccgcattc caccggccat gtcgagcagc tgggcgaacg
     5101 gcgtgccgag cttgatctcg taattgcccg gccgggcgac gtcgcccgac acggaaaaga
     5161 tcttcgtgcc gccgttgttc tggcgtccga gcgcgagaaa ggcctcgccg ccgttattga
     5221 tgatccacgg cacggcggcg aaggtctcgg tgttgttgat cgtggtcggc ttgccgtaca
     5281 ggccgaagct ggccgggaac ggcggcttgt agcgcggctg gcccttcttg ccttcgagcg
     5341 attcgagcag cgcggtttcc tcgccgcaga tgtaggcgcc gaagccgtgt gccgcgtgca
     5401 gctggaacga gtagccgctg ccgaacaggt tgtcgcccag gtagccggcg gcgcgcgctt
     5461 cctcgagcgc ttcctcgaag cgctcgtaga cctcgaagat ctcgc
//
LOCUS       DATANS010000067         2535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_674658, whole genome shotgun sequence.
ACCESSION   DATANS010000067 DATANS010000000
VERSION     DATANS010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674658"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VHT22_00990"
     CDS             <1..306
                     /locus_tag="VHT22_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6352 family protein"
                     /protein_id="HEX3136152.1"
                     /translation="LAVDVTIVPEREIVDARWVWHVGLDAEASALLNDLYNGVDVPDE
                     RRERLLCLFALRFSDPAAMRPAIAGRPVYLAMAMDRDNRLRLKPQNLLLNLPLARPQ"
     gene            320..931
                     /locus_tag="VHT22_00995"
     CDS             320..931
                     /locus_tag="VHT22_00995"
                     /inference="COORDINATES: protein motif:HMM:NF039391.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HEX3136153.1"
                     /translation="MIPKYLQSMLTTALLAGALVGLASAQAPATPPAVPPGGAASDKP
                     ADKPADKPADKPTGPLAPLAWMRGCWEGKVNQRDFREEWLPTASDMMIGVSQTTLRDK
                     TIDFEYLRLESRPEGVFYVAAPSGKNEAAFRLAGQTTSGGDEVFTFVSATPGAEYPQR
                     IIYRHATEGWLYATVEGKIKGMDKQVVFPMRRVDCQTGAVVGN"
     gene            952..1293
                     /locus_tag="VHT22_01000"
     CDS             952..1293
                     /locus_tag="VHT22_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136154.1"
                     /translation="MTDAATPAITRFEQLLAAGKDGALLRFALGSEYLKVGDAARASI
                     HLRQALAFDAGYSAAWKLLGQSLAAEGRTDEALAAYRDGIVAAERKGDKQAMKEMQVF
                     ARRIAKHHPPA"
     gene            1408..>2535
                     /locus_tag="VHT22_01005"
     CDS             1408..>2535
                     /locus_tag="VHT22_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010112116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX3136155.1"
                     /translation="MIKYDKLRRVVIGAPLDPLSQTTRQHIALAAFIAWVGLGADGLS
                     SSAYGPEEAFRALGAHTHLGLFMALATAATVFVIALAYNQVIELFPTGGGGYRVATSL
                     IGPRAGLVSGAALIVDYVLTIAISVASGVDALFSLLPAAIGHFKLATELLLVAVLLVL
                     NMRGMKESIRLLLPIFVGFCITHLLLIVYGVLGHSQGLTLVVPDALGESKKLVHDIGW
                     VAALSLFLRAYSLGGGTYTGIEAVSNNVQTLREPVVRTGKLTMFYMALSLAFTAGGII
                     LLYLLWQAKPVEGQTLNAVVFGSIIDSLGMSSGVNQVALLLVLLFEAGLLFVAANTGF
                     LGGPAVLSNMAADSWLPHQFRHLSTRRVTQNGIVIMGLAALG"
BASE COUNT          420 a          818 c          851 g          446 t
ORIGIN      
        1 ctggcggtag acgtcaccat cgtgccggag cgggagatcg tcgacgcgcg ctgggtttgg
       61 catgtcgggc tcgacgccga ggcaagcgcc ttgctcaacg acctgtacaa cggcgtcgat
      121 gtccccgacg agcggcggga acggctgctg tgcctgttcg cgttgcgatt ctccgatccg
      181 gcggcgatgc gaccggcgat cgccgggcgg ccggtatact tggcgatggc gatggatcgc
      241 gacaaccgcc tgcgattgaa gccacagaac ctgttgttga acctgccgct ggcgcggccg
      301 caatgaggga aaaggtacga tgattccaaa atatctgcag tcgatgctga ccaccgcatt
      361 gctcgcgggc gcgctcgtcg ggctcgcttc cgcgcaggct ccggcgacgc cgcccgccgt
      421 cccgcccggc ggcgcggcaa gcgacaagcc ggcagacaag ccggcagaca agccggcaga
      481 caagccgaca ggaccgctcg cgccactggc atggatgcgc ggctgttggg agggcaaggt
      541 taaccagcgc gactttcgcg aggaatggct gccgaccgcc agcgacatga tgataggggt
      601 cagccagacc acgctgcgcg acaagacgat cgatttcgaa tacctgcgac tggaatcgcg
      661 tccggagggc gtgttctacg tcgcggcgcc atccggcaag aacgaggcgg cgttccgcct
      721 cgcgggacag accacctccg gcggcgacga ggtgttcact ttcgtcagcg caacgcccgg
      781 cgcggaatat ccgcagcgca tcatctatcg ccatgccacc gaaggctggc tgtacgcgac
      841 cgtcgagggc aagatcaagg gaatggacaa gcaggtcgtc ttcccgatgc gccgcgtcga
      901 ttgccagacc ggcgctgtcg tcggcaactg atcccgcgac cggcgggcgc gatgacagac
      961 gcggcgacgc cggcaatcac gcgtttcgag caattgctcg ctgcgggcaa ggacggcgcg
     1021 ttgctgcgtt ttgccttggg cagcgaatac ctgaaggtcg gcgatgcagc gcgcgcgagc
     1081 atccatctgc gtcaggctct cgccttcgat gccgggtact cggccgcgtg gaagctgctc
     1141 ggtcaatcgc tggcggccga agggcgcacc gacgaagcgc tcgccgcgta tcgtgacggc
     1201 atcgtcgccg ccgagcgcaa aggcgacaag caggcgatga aggaaatgca ggttttcgcg
     1261 cggcgaattg ccaagcacca tccgccggcg tagagtgcgc cgatgctgca tcgcagcaaa
     1321 gctcttgccg ggttcccagt tgccgatacc gaggcctgcg gatttcagca atgacggccg
     1381 cgggctttgc cgctgcctga ctccgcgatg atcaaatacg acaagctccg ccgcgtcgtc
     1441 atcggcgcgc cgctcgatcc gttgagccag acgacgcgcc agcacatcgc gctggcagca
     1501 ttcattgcct gggtcgggtt gggcgccgac gggctgtcgt cgtcggccta cggccccgag
     1561 gaagcgttca gggcgcttgg cgcgcatacg catctaggcc tgttcatggc gctggccacc
     1621 gcggcgacgg tgttcgtcat cgcgctcgcc tacaaccagg tcatcgagct gtttccaacc
     1681 ggcggcggcg gctatcgcgt cgccaccagc ctgatcggcc cgcgcgccgg tctcgtgtcc
     1741 ggcgctgcgc tgatcgtcga ctacgtgctg acgatcgcga tatcggtcgc cagcggcgtc
     1801 gacgcgctgt tcagcctgct gcccgcggcg atcggccact tcaagctcgc aacggagctg
     1861 ctgctggtcg cggtgctcct ggtgctcaac atgcgcggga tgaaggagtc gattcgcctg
     1921 ctgctgccaa tcttcgtcgg cttctgcatc acccacctgc tgctgatcgt ctatggcgtg
     1981 ctcgggcact cgcaagggct gacgctggtc gtgcctgacg cgctgggaga atcgaaaaag
     2041 ctcgtgcacg acatcggatg ggtcgctgcg ctgtcgctgt tcctgcgcgc ctactcgctc
     2101 gggggcggaa cctacaccgg catcgaggcg gtgtcgaaca acgtccagac gttgcgcgag
     2161 ccagtggtgc gcaccggcaa gctgacgatg ttctacatgg cgctctccct tgcgttcacg
     2221 gcgggcggca tcatcctgct gtatctgctg tggcaggcga agccggtcga aggccagacg
     2281 ctgaacgccg tggtattcgg cagcatcatc gacagcctcg gtatgtcatc gggagtcaac
     2341 caggtggcgc tgctgctagt cctcctcttc gaggcgggac tgctgttcgt cgcggcgaac
     2401 acgggctttc tcggcggccc ggcggtgctg tccaacatgg cggccgactc gtggcttcca
     2461 caccagttcc gccatctgtc gacgcgtcgg gtcacccaga acggcatcgt catcatgggg
     2521 ttggcggcgc ttggg
//
LOCUS       DATANS010000068         2263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_634005, whole genome shotgun sequence.
ACCESSION   DATANS010000068 DATANS010000000
VERSION     DATANS010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634005"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VHT22_01010"
     CDS             <1..496
                     /locus_tag="VHT22_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEX3136156.1"
                     /translation="AFQTQLFFLFQRERQFRAIAQPSMFAQTIVSDFMFAKDALFAQL
                     NLSDEEYRLYAQMHQPIAAQLGEPDLVVRLQAAPPALLARVRRRGLAMEHGIDLAYLQ
                     RLCDAYAEFFAAYDGAPVFVVDTERFHPLERDADLAELVRALARFDGRRGVLGAAAAE
                     IAFD"
     gene            complement(744..2054)
                     /locus_tag="VHT22_01015"
     CDS             complement(744..2054)
                     /locus_tag="VHT22_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX3136157.1"
                     /translation="MPKCTDGRGGFGRIGRRVIEANFDGGDISSDGGLLLLRRADERI
                     GLSRAAAAVLSDPRDPSRITHSLRDLLAQRVYGLCCGYEDLNDHDALRDDLLMQTAVG
                     RVDALASSPTLSRLETRATRAQAVALHGVLIDQFIASHKAAPTELVLDIDASDVPLHG
                     SQERAEFHAYYDHHCYLPLYVFCGQAMLACVLRRSRIDGAKNAAAVIKLLVQRLRRAW
                     PDTRIIVRGDSGFCRQRLLRWCERSGVSYIIGLARNARLEARVRLAELMLADEYERSG
                     TKQRLIDEFTYAADSWDIERRVITRLEHGSQGNNPRFVVTNLEGNAVQLYERLYCARG
                     EAENRIKEAQLDLFGTRASCHRFAANQLRLLLAALAYTLMQRLRELALKGSELERATA
                     ATIRTRLLKIGAAIVRNTRRVRVLLASHHPLRALFLSAAQALAP"
BASE COUNT          356 a          769 c          746 g          392 t
ORIGIN      
        1 cgcgttccag acccagctct tcttcctgtt ccagcgcgag cgccagttcc gcgccatcgc
       61 ccagccgagc atgttcgcgc agacgatcgt cagcgacttc atgttcgcca aggatgccct
      121 cttcgcgcag ctcaacctca gcgacgagga gtaccgcctc tacgcccaga tgcaccagcc
      181 gatcgcggcg cagctcggcg aacccgatct ggtcgtgcgc ctgcaggcgg cgcccccggc
      241 gctgctcgcg cgcgtgcgcc ggcgcggcct cgcgatggag cacggcatcg acctagccta
      301 tctgcaacgg ctctgcgacg cgtatgccga attcttcgcg gcgtacgacg gcgcgcctgt
      361 cttcgtcgtc gacaccgagc gcttccatcc gctcgagcgc gacgccgacc tcgccgagct
      421 ggtgcgagcg ctcgcccgct ttgacgggcg gcgcggcgtc ctcggcgcgg cggccgcgga
      481 aatcgcgttc gactgacttc cgaagcgatc ggtcgccgcc cgatcgtcgc gatcgctgcg
      541 tcgtcggcgc gcgcgccgcg cgtgcgccta gcccggacat ttcgttagta ccgaatttcg
      601 cggcttgttc gaagtgatca gggcatttcg ggccgaacat ggctgtggag ggaggtgctg
      661 gatggggcaa gcgctgtttt tgcagacggc gcccccttac ccccgttgtt tgtcagcgtg
      721 ccggggacag cactggatga gtttcaaggg gccagggcct gcgcggcact gaggaacaag
      781 gcgcgcagcg gatgatgtga ggccagcagc acgcgcacgc ggcgggtgtt gcgcacgatg
      841 gcggctccga tcttgagaag ccgtgtgcgg atcgtcgccg ctgttgcccg ttcgagttca
      901 ctgcctttga gcgcaagctc gcgcagccgc tgcattagcg tgtaggccaa cgctgcaagc
      961 agcaagcgca gctggttggc cgcgaagcgg tggcagctgg cacgggttcc gaacaggtcg
     1021 agctgtgcct ccttgatgcg gttctcggcc tcgccccggg cgcagtacag ccgctcgtac
     1081 agctgtacgg cgttgccttc gagattggtc acgacgaagc gcgggttgtt gccttggctg
     1141 ccatgctcga gccgagtgat cacgcgacgc tcgatgtccc aactatcggc cgcgtaggtg
     1201 aactcgtcga tcagccgctg cttggtgccg ctgcgctcgt actcgtcggc cagcatcagt
     1261 tcggccaagc gaacccgcgc ttccaggcgg gcgttgcgag ccagcccgat gatgtagcta
     1321 acgcccgagc gctcgcacca gcgcagcaag cgctggcggc agaacccgga gtcgccgcgc
     1381 acgatgattc gtgtatcagg ccaggcccgg cgcagccgct gcaccagcag cttgatcacg
     1441 gccgcggcgt tcttggcccc atcgatgcga ctgcgccgca gcacgcacgc caacatcgct
     1501 tggccgcaga acacgtacag cggcaggtag cagtggtggt cgtagtaggc atggaactcg
     1561 gcacgctcct ggctgccgtg cagcggcacg tcggaggcgt cgatgtcgag cacgagttca
     1621 gtcggtgccg ccttgtggct ggcgatgaac tgatctatga gcacgccatg caatgcaacg
     1681 gcttgcgccc gcgtcgcccg cgtctccagg cggctcagcg ttggcgagct cgccagcgca
     1741 tcgacccggc cgaccgcggt ttgcatcagc aagtcgtctc gcaacgcgtc gtggtcgttg
     1801 aggtcctcgt agccgcagca caggccgtag acgcgctggg ccaggagatc tcgcaggctg
     1861 tgcgtgatgc gcgacgggtc gcgcggatca ctcagcaccg ccgcagccgc gcggctcaaa
     1921 ccgatccgct cgtcggctcg tcgcaatagc agtagaccgc catccgagct gatgtcgccg
     1981 ccatcgaagt tggcctcgat cacgcgtcga ccaatacgcc caaatccacc ccgcccatcg
     2041 gtacactttg gcatcggcag tcctcggttg tcattggcgt cgaataccta tgacaacgcg
     2101 cgcaagcacg aggctgccga ccctcttctg gtgaattttc cgggctaatc ccggtcgggc
     2161 gccacgtcga gggccacgcc gagcaccgcg gcgatgtcgc ggcgcagttg cccggccagc
     2221 gcacgccgat cagcgccggc agggtcgcgc ggcgccagga aca
//
LOCUS       DATANS010000069         2838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_759362, whole genome shotgun sequence.
ACCESSION   DATANS010000069 DATANS010000000
VERSION     DATANS010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759362"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VHT22_01020"
     CDS             complement(<1..574)
                     /locus_tag="VHT22_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011046954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3136158.1"
                     /translation="MFAYIGRRLLATIPVMTVVAVFVFMMLRLASGDPAAIIAGNAAT
                     AQDIVDIRQKLGLDRPIVTQFFIWLGHVLTGDFGESFFYKKQVAEIIADRAGPTLALA
                     ACTLVISVLVAVPLGVVAAYRRGTWLDRVVMGFSVLGFSVPVFVIGYALIYLFAIKLN
                     WLPVQGYQPLAEGIGGFFARLILPSVTLSVI"
     gene            complement(628..2220)
                     /locus_tag="VHT22_01025"
     CDS             complement(628..2220)
                     /locus_tag="VHT22_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3136159.1"
                     /translation="MPISRWLKTMALAAVAGFAALSGTPNAAAQGTVRAVMHSDLKIL
                     DPIWTTAYIQRNYGYMVYDTLFATDADGQVKPQMIEHYEVSPDQLTYTMTLRDGLLWH
                     DGKPVTAEDCVASIKRWGAKDTMGQKLMTFVKDMPVVDARTFRIVLSSPTGLVLAGLG
                     KPSSNTPFMMPKRVAETSPSEQISDTTGSGPFVFKRDEWKPGDKAVFVKFDKYKPRAE
                     PPSGLAGGKVVKVDRVEWKVVADQQTAMNALFAGEVDYIEQPQHDLLPLMKADKNVKL
                     VDVNPLGNQYALRFNSTFKPFDNPKIRQAALYALNQEDFLKATIGDPQYYKVCKAMFV
                     CGAPLASTKGMDGLLESNFAKSKALLKEAGYDETPVVLLHSTDLQVLTNLAPVAKSLL
                     EKGGFKVDMQSMDWQSVVARRAKKDPPAQGGWNAMLTSWVSADILNPVSTAFLNASCD
                     KAPFGWPCDAELERLRDQFARETDPVKQKAIAEAVQVRWTQSPTHVFLGQWYQPSAVR
                     KNIDGIIVAPVPVFWNMTKSGA"
     gene            complement(2379..>2838)
                     /locus_tag="VHT22_01030"
     CDS             complement(2379..>2838)
                     /locus_tag="VHT22_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX3136160.1"
                     /translation="PEFREYMLPPIEKEGDQGIRTAVRFMLENLASRDALPYMNALAE
                     RARLVREWTAFLDRVPLVLAPVSSVPVYEQGFDVESAARTAAVWRECATLMAIPVLGF
                     PAAVVPTGIADGLPTAVQLIAPRFREDWCLDAAAAIEARAGVLTPVDPQW"
BASE COUNT          506 a          945 c          895 g          492 t
ORIGIN      
        1 agatgaccga caaggtgacg ctgggcagga tcagtcgcgc gaaaaagccg cctatgccct
       61 cggccagcgg ctggtagccc tgcaccggca gccagttgag cttgatggca aacaggtaga
      121 tcagcgcgta gccgatgacg aataccggca ccgaaaagcc gagcaccgaa aagcccatga
      181 cgacgcggtc gagccatgta cctcggcggt atgccgcgac gacgcccagc ggaacggcga
      241 cgagcaccga aatgacgagc gtgcatgcgg ccagcgccag ggtcggaccg gcgcgatcgg
      301 cgatgatctc cgcgacctgt ttcttgtaga agaacgattc gccgaaatcg ccggtcagca
      361 catggccgag ccagatgaag aattgcgtca cgataggccg atcgagcccg agcttttgcc
      421 ggatgtcgac gatgtcctgc gcggtcgccg cgttgccggc gatgatcgcc gccggatcgc
      481 cggaggccag ccgcagcatc atgaagacga acaccgcgac gacagtcatc accggaatcg
      541 tcgcgagcag gcgtcgtccg atgtaggcga acatgggcta gtcgccagcg cgccggatgc
      601 cgccccgtcg ttgcggggag gcgacgctta cgcgccggac ttggtcatgt tccagaacac
      661 cggaaccggc gcgacgatga taccgtcgat gttcttgcgc acggccgacg gctggtacca
      721 ctggccgaga aagacatgcg ttggagactg cgtccagcgc acttgcaccg cttccgcgat
      781 cgccttctgc ttcacggggt cggtttcgcg cgcgaactgg tcgcgcaggc gctccagctc
      841 ggcgtcgcac ggccagccga acggcgcctt gtcacaactg gcgtttagga acgccgtcga
      901 caccggattc aggatgtcgg cggaaaccca cgacgtgagc atcgcgttcc agccgccttg
      961 cgccggcgga tcctttttcg cgcgccgcgc cacgaccgac tgccagtcca tcgactgcat
     1021 gtcgaccttg aatccgccct tttccagcag cgatttggcc accggcgcga ggttggtcag
     1081 cacctgcagg tcggtcgaat gcaacagcac caccggcgtt tcgtcgtagc cggcttcctt
     1141 cagcagcgct tttgacttgg cgaagttgga ctccagcaat ccatccatgc ccttggtcga
     1201 cgccagcggc gcgccgcaaa cgaacatcgc cttgcacacc ttgtaatact gcgggtcgcc
     1261 gatcgtcgcc ttcaggaaat cctcctgatt gagcgcatag agcgccgcct gccggatctt
     1321 cgggttgtcg aacggtttga acgtcgagtt gaaccgcagc gcgtactggt tgcccagcgg
     1381 attgacgtcg acgagcttga cgttcttgtc cgccttcatc agcggaagca ggtcgtgctg
     1441 cggctgctcg atgtagtcga cctcgccggc aaatagcgcg ttcatcgcag tctgctggtc
     1501 ggcgacgact ttccattcga cgcggtcgac cttgaccact ttgccgccgg ccaggcccga
     1561 aggcggttcg gcgcgcggct tgtacttgtc gaacttgacg aacaccgcct tgtcgccggg
     1621 cttccactcg tcgcgcttga atacgaacgg gccgctcccg gtcgtatccg aaatctgctc
     1681 gctcggactg gtttcggcga cgcgcttggg catcatgaac ggcgtgttcg acgatggctt
     1741 gcccaggccc gcgagcacca gcccggtcgg cgaagacaga acgatccgga acgtcctggc
     1801 gtcgacgacc ggcatgtcct tgacgaaggt catcaacttc tgccccatcg tgtccttcgc
     1861 gccccagcgc tttatcgagg cgacgcagtc ctcggcggtc accggcttgc cgtcgtgcca
     1921 caacaggccg tcgcgcaagg tcatcgtgta ggtcagttgg tctgggctga cctcgtagtg
     1981 ctcgatcatc tgcggcttca cctggccgtc tgcgtcggtg gcgaacagcg tgtcgtagac
     2041 catgtaaccg tagttgcgct ggatgtaggc cgtcgtccag atcggatcga ggatcttcag
     2101 gtccgaatgc atgacggcgc gcaccgtgcc ctgggccgcg gcgttcgggg ttcccgacaa
     2161 cgcggcgaat ccggcaaccg ccgcgagcgc catcgtcttg agccagcggg aaatgggcat
     2221 cgagttctcc tgtctgcgaa gctgtcgccg ccgcgaggcg ccgaacgtgc acgatagccg
     2281 aagcgtggcc ggcatggcaa tggccgcctt ggcgtgacga gggcagacag cgtgcgcgcc
     2341 gcaacgcgcc accgcatcgc atacgtcgag gcgcttgttc accattgggg atcgaccggc
     2401 gttagcacgc cggcgcgcgc ctcgatcgcg gccgccgcgt cgaggcacca gtcttcgcga
     2461 aagcgcggcg ctatcagctg cacggcggtc ggcaagccgt ccgcaatgcc ggtcggcacg
     2521 accgccgccg gaaatccgag caccggtatc gccatcaagg tagcgcactc gcgccaaacc
     2581 gccgcggtgc gcgccgcgct ttcgacgtcg aacccctgct cgtacaccgg caccgacgaa
     2641 accggcgcca gcaccagcgg cacgcgatcg aggaaggccg tccactcccg caccaggcgc
     2701 gcgcgctcgg ccagcgcgtt catgtacggc agcgcgtcac gcgacgccag gttctccagc
     2761 ataaaacgca ccgccgtccg gatgccctgg tccccttcct tttcgatcgg cggcagcatg
     2821 tactcgcgaa actcaggc
//
LOCUS       DATANS010000070         2581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_3456, whole genome shotgun sequence.
ACCESSION   DATANS010000070 DATANS010000000
VERSION     DATANS010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3456"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /gene="aceE"
                     /locus_tag="VHT22_01035"
     CDS             complement(<1..1411)
                     /gene="aceE"
                     /locus_tag="VHT22_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231577.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX3136161.1"
                     /translation="MDINPDVDPQETREWLDAIDGVLENEGPDRAHFLIEQVIDKARR
                     SGAYMPFSANTAYINTIPVDKQVRIPGDQAIEHRIRSYVRWNAMAMVVRANKHTNVGG
                     HIASFASAATLYDTGFNHFWHAPSPEHGGDLVFVQGHSSPGVYARAHMLGRFTDEQMD
                     NFRQEVDGKGVSSYPHPWLMPDFWQFPTVSMGLGPLMAIYQARFMKYLQDRGLAKTEG
                     RKVWCFCGDGEMDEPESMGAIGMAARESLDNLIFVINCNLQRLDGPVRGNGKIIQELE
                     ADFRGAGWNVIKVIWGSRWDPLLARDKQGILMRRMMECVDGEYQTFKSKDGAYVREYF
                     FNTPELKALVADYSDEEIWNLNRGGHDPHKVYAAYRAAVDHKGQPTIILAKTIKGYGM
                     GEAGEAQNITHQQKKMSGGSVRHFRDRFQIPVPDDKLDELPYVKFPPGSAEAEYMMAR
                     RMELGGFLPTRRRKSASLAV"
     gene            complement(1582..2205)
                     /locus_tag="VHT22_01040"
     CDS             complement(1582..2205)
                     /locus_tag="VHT22_01040"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX3136162.1"
                     /translation="MVQSSFAHSTPTADALREAKRSLRERVLAARDSLSPEFRAAASA
                     AIGTALAARDDFAAASTVLLTLPFGSEWDSMALLLAALERGKAVVLPRVNATARTLEL
                     CRLSEPSRDVLPGYRGIPEPQSHCALIAAEAIDWVLVPGVAFDIAGHRLGYGGGYYDR
                     LLPQLRSDAARIAGGYEMQLVDRVPAAPHDVPVQALATETRTLPMPE"
     gene            2292..>2581
                     /locus_tag="VHT22_01045"
     CDS             2292..>2581
                     /locus_tag="VHT22_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEX3136163.1"
                     /translation="MNHSHQHHRARRARLIAEMRRQSGGGIAAIPTAPEAFRNADTHY
                     PYRPDSHFYYLCGFPEAEAVVVLLAGADEGDSKQILLCRDKNPEREIWDGFR"
BASE COUNT          443 a          859 c          828 g          451 t
ORIGIN      
        1 gcaccgcgag cgacgcggac ttcctgcggc gcgtcggcaa aaagccgccg agctccatgc
       61 gccgggccat catgtattcg gcctccgccg atccgggcgg aaacttgacg tacgggagct
      121 cgtcgagctt gtcgtcgggc accggaatct ggaagcgatc gcgaaagtgc cggaccgagc
      181 cgcccgacat tttcttctgt tggtgcgtga tgttctgcgc ctcgccggcc tcacccatgc
      241 cgtagccctt gatcgtcttg gcgagaatga tcgtcggctg ccccttgtgg tcgacggcag
      301 cccggtaggc ggcatagacc ttgtgcggat cgtggccgcc gcgattcaga ttccagatct
      361 cctcgtccga gtagtcggcc accagcgcct tgagctcggg ggtgttgaag aaatattcgc
      421 gcacgtaggc gccgtccttc gatttgaagg tctggtattc gccatcgacg cattccatca
      481 tccggcgcat caggatgcct tgcttgtcgc gcgccagcag cgggtcccag cgcgagcccc
      541 agatcacctt gatcacgttc cagccggcgc cgcggaagtc ggcctccagc tcctggatga
      601 tcttgccatt gccgcgcacc gggccgtcga gccgttgcag gttgcagttg atgacgaaga
      661 tcaggttgtc cagcgactcg cgggccgcca ttccgatcgc gcccatcgat tccggctcgt
      721 ccatttcgcc gtcgccgcaa aaacaccaga ccttgcgccc ctcggtcttg gccaggccgc
      781 gatcctgcag atacttcatg aaacgcgcct ggtagatcgc catcagcggg ccgagcccca
      841 tcgatactgt cgggaactgc cagaagtccg gcatcagcca cggatgcgga tacgacgaga
      901 cgcccttgcc gtcgacttcc tgccggaagt tgtccatctg ctcgtcggtg aagcggccca
      961 gcatgtgggc gcgggcgtac acgccgggtg acgaatggcc ctggacgaac acgaggtcgc
     1021 cgccgtgctc gggcgagggc gcgtgccaga agtgattgaa accggtgtcg tagagcgtcg
     1081 ccgccgaggc gaagctcgcg atgtggccgc cgacgttggt gtgcttgttc gcccgcacca
     1141 ccatcgccat cgcgttccag cgcacataag accggatgcg atgctcgatc gcctggtcgc
     1201 ccggaatccg gacctgctta tcgaccggaa tcgtattgat gtaggcggtg ttcgccgaaa
     1261 acggcatgta cgcgccggat cggcgcgctt tgtcgattac ctgttcgatc aggaaatgcg
     1321 cgcggtccgg accttcgttc tcgagcacgc cgtcgatcgc gtccagccat tcgcgcgttt
     1381 cctgcgggtc gacatcgggg ttgatatcca tgcgggcact ccttcggcga tcctacaagc
     1441 ggtcgcgcga aatttcgatt gtaggcgtca cagcatggcg ccgcattgct gcgacgcgac
     1501 actcgccggc tcagggctga ttcgaggata ccacggcgtg gaaatgattt ccaaaactgc
     1561 cccgggcacg cggtcggccg gctactcagg catcggcaag gtccgggtct cggttgcgag
     1621 cgcctgcacc ggcacgtcgt gcggtgcagc cggcactcga tcgaccaact gcatttcgta
     1681 gccgccggca attctcgctg cgtcgctgcg cagttgaggg agcagcctgt cgtagtagcc
     1741 gccgccatag ccgaggcggt gccctgcaat gtcgaacgca acgccgggga ccagcaccca
     1801 atcgatcgcc tcggcagcga tcagcgcgca gtgcgattgc ggctcgggta tgccgcgata
     1861 gccgggcagg acgtcgcgtg agggttcgct cagccggcaa agctccagcg tgcgcgctgt
     1921 cgcgttgacg cgcggcagca cgaccgcctt gccgcgttcc agcgcggcga gcaacagcgc
     1981 catcgagtcc cactcgctgc cgaacggcag tgtcagcagc acggtcgacg ccgcggcgaa
     2041 atcgtcgcga gccgcgagcg ccgtgccgat cgccgcgctt gccgccgcgc gaaactccgg
     2101 cgacagggaa tcgcgcgcgg cgagcacgcg ctcgcgcagc gaacgcttgg cctcgcgcag
     2161 cgcatcggcg gtgggcgtcg aatgagcgaa ggaagactgc accacgaagc ggatgttacc
     2221 gcaggctcgg cgagaggagc cgctgcgcct ataatggcgc cttccccccg acacgccgcg
     2281 caatcccaac gatgaaccac agtcaccagc accaccgtgc gcgccgcgcg cgattgatcg
     2341 ccgagatgcg ccgtcagagc ggcggcggga tcgccgcaat cccgacggcg cccgaagcgt
     2401 tccgcaacgc cgacacccac tatccgtacc ggccggacag ccatttctat tacctttgcg
     2461 gatttcccga agccgaagcc gtcgtggtgc tgctcgccgg ggcggacgaa ggcgactcga
     2521 agcagatcct gctttgccgg gacaagaatc ccgagcgcga gatctgggac ggctttcgct
     2581 a
//
LOCUS       DATANS010000071         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_466012, whole genome shotgun sequence.
ACCESSION   DATANS010000071 DATANS010000000
VERSION     DATANS010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466012"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /gene="fabF"
                     /locus_tag="VHT22_01050"
     CDS             <1..1143
                     /gene="fabF"
                     /locus_tag="VHT22_01050"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX3136164.1"
                     /translation="SGIVAITRFDASAFPSRIAGEVKDFDVTKYLSGKDVRRYDRFVH
                     YGQVATMQAVTDAGLDGYGGNLERVGVCIGSGIGGLPMIEDTHNAYLQNGLRKISPFF
                     VPGSIINMISGLVSIHYGYQGPNLATVSACSSANHSLGEAARLIEYGDADIMVAGGAE
                     GTVSPLGIGGFCAARALSNRNDDPATASRPWDIDRDGFVLGEGSGVLVLEELEHAKAR
                     GARIYCELVGYGMSADAHHITAPPEDGGGAARSMVNALKNGGLDVRDIDYINAHGTST
                     QLGDVAECVAVKRCFGEHASRLAVSSTKSMTGHLLGAAGGVEAVFTVLALRDQVAPPT
                     ANLFHRDPRCDLDFVPNEARKMKIRAALSNSFGFGGTNATLAFRLL"
     gene            1193..>1608
                     /locus_tag="VHT22_01055"
     CDS             1193..>1608
                     /locus_tag="VHT22_01055"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058944.1"
                     /note="catalyzes the formation of 4-phospho-L-aspartate
                     from L-aspartate and ATP, in Bacillus, lysine sensitive;
                     regulated by response to starvation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX3136165.1"
                     /translation="MALIVQKYGGTSVGSIERIRNVARRVAHFHRAGHQLVVVPSAMS
                     GETNRLLALARELAPNPDPRELDVVAATGEQVTIGLLAIALQQLGLQAKSYTGGQVQV
                     LTDSAFTKARILKIDEERIRRDLADGRVVIVAGFQG"
BASE COUNT          271 a          528 c          537 g          272 t
ORIGIN      
        1 tcgggcatcg tcgcgatcac gcgcttcgat gccagcgcct ttccttcccg catcgcggga
       61 gaggtcaagg atttcgacgt caccaagtac ctttccggca aggacgtgcg ccgctacgac
      121 cggttcgtcc attacggcca ggttgccacg atgcaggcgg tgaccgacgc cggcctcgac
      181 ggctatggcg gcaacctcga gcgggtcggc gtatgcatcg gctcgggaat cgggggcctg
      241 ccgatgatcg aggacacgca caatgcctat ttgcagaacg ggctccgcaa gatctcgccc
      301 ttctttgtgc cgggatcgat catcaacatg atctcgggtc tggtgtcgat ccactacggc
      361 taccaggggc cgaacctggc caccgtcagc gcctgctcgt cggccaatca cagcctgggc
      421 gaagcggcgc gcctgatcga atacggcgac gccgacatca tggtcgccgg cggcgccgag
      481 ggaacggtga gcccgctggg catcggcggc ttctgcgccg cacgtgcact gtcgaaccgc
      541 aacgacgatc ctgcgacggc aagccgaccc tgggacatcg accgcgacgg cttcgttctg
      601 ggtgaaggct cgggcgtgct cgtgctcgag gaactcgagc acgccaaggc tcgcggagcg
      661 aggatctatt gcgagctggt gggttacggc atgtcggccg acgcccacca catcaccgca
      721 ccgccggagg atggcggcgg cgccgcgcgc agcatggtta acgcactgaa gaacggcggg
      781 ctcgatgtgc gcgatatcga ctacatcaac gcgcacggta cttctactca gcttggcgat
      841 gtggccgaat gcgtcgcagt caagcgttgc tttggcgagc atgcgtcgcg gctggcggtc
      901 tcgtcgacca aatcgatgac cggacatctg ctcggcgccg cgggcggtgt cgaggccgtg
      961 tttaccgtgc tcgcgctgcg cgatcaggtc gcaccgccga ccgccaactt gttccatcgc
     1021 gacccgcgtt gcgatctcga cttcgtgccg aacgaggcgc gcaagatgaa aatccgcgcc
     1081 gcactgtcga attcgttcgg cttcggcgga acgaacgcga cgctcgcctt ccggttgctg
     1141 tgagcggcgt gcgcgcgcat cgcgggcgtg atgccgtcga ccgaaggctg ccatggcgct
     1201 gatcgtccag aagtacggcg gcacctcggt cggcagcatc gagcggatcc gcaacgtcgc
     1261 gcggcgggtc gcgcatttcc accgcgccgg ccatcagctg gtcgtggtgc cgtcggcgat
     1321 gagcggggag accaaccgcc tgctcgcgct cgcccgtgag ctggcgccca atcccgatcc
     1381 ccgcgagctc gacgtcgtcg cggcgaccgg cgagcaagtg actatcggcc tgctggcgat
     1441 cgccttgcag caactcgggt tgcaggccaa gagctacacc ggcgggcagg tgcaggtgct
     1501 gaccgacagc gcgttcacca aggcgcgcat cctgaaaatc gacgaagagc gcatacgccg
     1561 cgaccttgcc gatggcaggg tcgtcatcgt cgccggcttt cagggcat
//
LOCUS       DATANS010000072         1508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_172227, whole genome shotgun sequence.
ACCESSION   DATANS010000072 DATANS010000000
VERSION     DATANS010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172227"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VHT22_01060"
     CDS             <1..404
                     /locus_tag="VHT22_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136166.1"
                     /translation="VGGAVLMLIGTALILKGAAASVLIRQSVLETWIKPGVSLGVAVG
                     ALVLLLAVWLPRPVRTTLCAISLLASLVPPLLAPDMWRARAPLALFDWPYGQLANFNG
                     LTRAVLVAWPILASMYVLSVAARRDHADESD"
     gene            456..1061
                     /locus_tag="VHT22_01065"
     CDS             456..1061
                     /locus_tag="VHT22_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009908400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3136167.1"
                     /translation="MTRGEQRLSIAGPSGALELALNLPEQAPRGIALIAHPHPLQGGT
                     MDNKVVQTLAKTFAGLRYASVRFNFRGVAGSAGVFDDGAGETDDALAALAHGRTAYGD
                     LPVVLAGFSFGTFVQTRVAQQVTAERMVLIAPAVARFGTGAVPADTIVIHGEEDDVVG
                     LVDVFAWARPQQLPVVVFPGCGHFFHGRLPQLTRVIAGMWR"
     gene            1112..>1508
                     /locus_tag="VHT22_01070"
     CDS             1112..>1508
                     /locus_tag="VHT22_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3136168.1"
                     /translation="MLAAAGLCKSYGDNEVVRGLDFAIRRGECFGLLGPNGAGKTTTL
                     RCCLGLTDPNAGTITLVGEPVPQAARQARIRVGVVPQMDNLDPDFTVTENLRIYGRYF
                     GIPTATLSERIPRLLDFAGLASKGAMSIRT"
BASE COUNT          210 a          509 c          515 g          274 t
ORIGIN      
        1 tcgtcggcgg cgccgtgctg atgctgatcg gcacggcgct gatcctcaag ggcgctgcgg
       61 cgagcgtgct gatcaggcag tcggtgctgg agacgtggat caagccgggg gtctcgctcg
      121 gtgtcgccgt cggcgcgctg gtgctgctgc tcgcggtctg gctgccgcgg ccggtccgga
      181 cgacgctgtg cgcgatctcg ctgctggcct cgctggtccc gccgctgctg gcgcccgaca
      241 tgtggcgcgc gcgcgcgccg ctcgctcttt tcgattggcc ctacggacag ctggccaatt
      301 tcaacgggct gacgcgcgcg gtgctcgtcg cctggccgat actggcgagc atgtatgtgc
      361 tgtcggtcgc cgcgcggcgt gatcacgccg acgaaagcga ctagcaccac gccgcgtata
      421 attttgcgct tcacgagttg tgggcccatt ccctgatgac gcgtggcgaa cagcgcctgt
      481 cgatcgccgg cccttccggc gcgctcgagc tcgcgctcaa tctgcccgaa caggcgcctc
      541 ggggcattgc gctgatcgcg cacccgcatc cgctgcaggg cggcacgatg gacaacaaag
      601 tcgtgcagac gctcgccaag acgttcgcgg ggctgcgcta cgccagcgtg cgattcaatt
      661 ttcgcggcgt ggcggggagc gccggcgtat tcgacgacgg cgccggcgaa accgatgatg
      721 cgctggccgc gctggcccac ggccgcaccg cgtatggcga ccttcccgta gtactcgcag
      781 gattctcgtt cgggacattc gtccagacac gcgttgcgca gcaagtgacc gctgagcgca
      841 tggtgctgat cgcacccgca gtcgcgcgat tcggcacagg cgccgttcct gccgatacga
      901 tcgtgatcca tggcgaagag gacgacgtgg tcgggctcgt cgatgtgttt gcctgggcgc
      961 gcccgcaaca gctgcctgtc gtcgtgtttc ccggctgcgg ccatttcttt cacggccggc
     1021 tgccgcagct gacgcgggtc atagccggca tgtggcggtg atcgcggcgc ccgggccgga
     1081 ggccgccggc acgcagcgac gcgacgatgt tgtgctcgcc gcggctggcc tgtgcaagtc
     1141 gtacggcgac aacgaggtcg tgcgcggtct cgatttcgcg atccgtcgcg gcgaatgctt
     1201 cggcctgctc ggccccaatg gcgcggggaa gacgacgacc ttgcgctgct gcctcggact
     1261 caccgacccc aacgccggca cgatcacgct ggtcggcgag ccggtgcccc aggccgcgcg
     1321 tcaagcgcgc attcgcgtcg gcgtcgtgcc gcagatggac aacctcgatc ccgacttcac
     1381 cgtgaccgag aatcttcgca tctacggccg ctatttcggc attcccactg cgaccttgtc
     1441 cgaacgcata ccccggctgc ttgactttgc gggtctagcg agcaagggag cgatgagcat
     1501 tcgcacgc
//
LOCUS       DATANS010000073         2282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_172257, whole genome shotgun sequence.
ACCESSION   DATANS010000073 DATANS010000000
VERSION     DATANS010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172257"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="VHT22_01075"
     CDS             <1..794
                     /locus_tag="VHT22_01075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX3136169.1"
                     /translation="IVLSGASGANDGWLRARETGLALKQAVRRATGLSCSIGIAPNKL
                     LAKIASELDKPDGLTLLPTDDIAARVWPLPARRINGIGPKSSARLQVLGIRTIGELAA
                     ADPALLLEHFGAHYGAWMHEAAHGRDERPVVTYSEPQSISRETTFDRDLHPSRDRDEL
                     SAIFDELCAGVSDDLTRKGYVGRTIGLKLRYDNFKTLTRDRTIPQPTQDARTIRRAAS
                     ECLKRVPLDRRIRLLGVRVGALCRADEAARPVTVAAEEPTRNLFE"
     gene            complement(804..1367)
                     /locus_tag="VHT22_01080"
     CDS             complement(804..1367)
                     /locus_tag="VHT22_01080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX3136170.1"
                     /translation="MNSPRDVVVIVGSLRKESFNRKMAKGIAALAPPTLKLEIVEIGQ
                     LPLYNQDLEASPTAEVTAFKERVARADAVLFVTPEYNRGVPGVLKNAIDVASRPYGKS
                     AWNGKPGAVVSVSPGAIGAFGANHQLRQSLVFLNVPALPHEAYIGGAGKLFDDKGALT
                     SDDTREFLKKFLQLFEKWIEANAAPRA"
     gene            complement(1419..1643)
                     /locus_tag="VHT22_01085"
     CDS             complement(1419..1643)
                     /locus_tag="VHT22_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136171.1"
                     /translation="MTPENEHPASAEIERLVAELEQAEAYEQKLRQLIVEVRDALAAG
                     RIEQALSMLNTALSDIDSATDVVVPPHRTK"
     gene            1829..>2282
                     /locus_tag="VHT22_01090"
     CDS             1829..>2282
                     /locus_tag="VHT22_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3136172.1"
                     /translation="MAIALAMVVVLTACGTAAFQHEESRLTFRVVRDTPGWYTGMPDG
                     VEEFDLPVASGEAVEHIHAWWWPAAGGRADAPAILYLHGARWSLTGQLFRIEELRDFG
                     FSVLAIDYRGFGKSDGDLPSEQTVYEDARAGWRWLVAHEPDAARRFIYG"
BASE COUNT          349 a          771 c          763 g          399 t
ORIGIN      
        1 gtatcgtcct ttccggggcg agcggcgcga acgatggctg gctgcgcgca cgcgagaccg
       61 ggctcgcgct caagcaggct gtgcgccgcg ccaccggact gtcgtgctcg atcgggatcg
      121 cgccgaacaa gctgctggcc aagatcgcct ccgagctcga caagcccgac ggcctgacct
      181 tgctgccgac ggacgacatc gccgcgcgcg tctggccgtt gccggcgcgc aggatcaacg
      241 gcatcggccc gaagtccagt gcccgactgc aagtattggg catacgtacg ataggcgagc
      301 tcgcggccgc cgatcccgcg ctgctgctcg agcatttcgg cgcgcattac ggcgcgtgga
      361 tgcacgaggc cgcgcacggc cgcgacgaac gcccggtggt cacctacagc gagccgcaat
      421 cgatcagccg cgagacgacg ttcgatcgcg acctgcatcc gtcgcgcgac cgcgacgagc
      481 tctccgccat tttcgacgag ctctgcgccg gcgtcagcga cgatctcacg cgcaaaggtt
      541 acgtcggccg gacgatcgga ctcaagctcc gctacgacaa tttcaagacg ctgacgcgcg
      601 accggacgat tccccagccg acgcaggacg cgcgcacgat ccggcgcgcc gcaagcgaat
      661 gcctgaagcg cgtgccgctg gaccgccgaa ttcgtctgct cggcgtcagg gtcggcgcgc
      721 tctgccgcgc cgacgaggcg gcgcgtccgg taacggtcgc ggcggaagag ccgacgcgaa
      781 acctgttcga gtagcgccag cgactaggcg cgcggcgccg cgttggcctc gatccacttt
      841 tcgaacagct gcaggaactt cttcaggaac tcgcgcgtgt cgtcgctggt cagcgcgccc
      901 ttgtcgtcga acagcttccc ggcgccgccg atgtaggcct cgtgcggcag cgccggcacg
      961 ttgagaaaca ccagcgattg acgtagctgg tggttggcgc cgaacgcgcc gatcgctccg
     1021 ggcgagacgc tgaccactgc gccgggcttg ccgttccacg cgctcttgcc gtacggacgt
     1081 gaggcgacgt cgatcgcatt cttcaggacg ccaggcacgc cgcggttgta ttcgggggtc
     1141 acgaacagca ctgcgtcggc gcgcgcaacg cgctccttga atgcggtgac ttcggcggtc
     1201 ggactggcct cgagatcctg gttgtacagc ggcagctggc cgatctccac gatttcgagc
     1261 ttcagcgtcg gcggcgccag cgctgcgatc cccttggcca ttttgcggtt gaacgattcc
     1321 ttgcgcaggc tgccgacgat gacgacgacg tcgcgggggg agttcattgg cgttcctttc
     1381 gtggaatgag cggcggggac cggctgccgg cccggagact atttcgtgcg gtgcggcgga
     1441 acgaccacgt cggtggcgct gtcgatatcg ctcagcgccg tgttcagcat cgaaagcgcc
     1501 tgctcgatcc gccccgccgc aagcgcgtcc ctgacttcga cgatcagctg gcgcagcttc
     1561 tgctcgtagg cttcggcctg ctcgagctcg gcgacaaggc gttcgatctc ggctgacgca
     1621 gggtgctcgt tttcgggagt catggcctgg tgacggcgtt tcgcaccggc cctggtcggc
     1681 cggaatcaca agtaaagtag acgcggctat ggccgcgccg tcaacgcgct ttgcctgcgg
     1741 cgcgtttttt cttgacacct aaccggatcg atcgaagcag cccgatacct ccatggactt
     1801 cctcttgcgc aaaccgttcc tccgccgcat ggcgatcgcc ctggcgatgg ttgtcgtgct
     1861 gaccgcctgc ggcaccgccg ccttccagca tgaggaatcc cggctcacct ttcgcgtggt
     1921 ccgcgacacg cccggttggt acaccggcat gccggatggc gtcgaggaat tcgatctgcc
     1981 cgtggcaagc ggtgaagctg tcgagcatat ccatgcctgg tggtggcccg ccgccggcgg
     2041 cagggccgac gcgccggcga tcctgtatct gcacggcgcg cgctggagcc tgaccggcca
     2101 gctgttccgc atcgaggagc tgcgcgactt cggcttttcg gtgctggcga tcgactatcg
     2161 cggcttcggc aagagcgatg gcgacctgcc ttcggagcag accgtgtacg aggatgcgcg
     2221 cgccggctgg cgctggctcg tcgcacacga gcccgacgcg gcccgccgct tcatctacgg
     2281 gc
//
LOCUS       DATANS010000074         2139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_424324, whole genome shotgun sequence.
ACCESSION   DATANS010000074 DATANS010000000
VERSION     DATANS010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424324"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VHT22_01095"
     CDS             <1..382
                     /locus_tag="VHT22_01095"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEX3136173.1"
                     /translation="EKSDVHVHVPAGATPKDGPSAGVAMIVALVSLLTDRPVRTDVAM
                     TGEISLRGLVLPIGGVKEKVLAALRAGIRTVMLPSRNRRDLEEIPPEARAQLAFVWLE
                     EVDDAIAAALAPGESQAVPVPDPT"
     gene            complement(395..1606)
                     /locus_tag="VHT22_01100"
     CDS             complement(395..1606)
                     /locus_tag="VHT22_01100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3136174.1"
                     /translation="MNTPRAAPSSASTAAIALSATLAIQVFTALSGAAIAVLAPEIAR
                     DFGVPAKLVGIFVGLLYVGSMTASLASGHFIARRGAIRVSQLCVLLCAAGILLVALLP
                     SRAAAMLAVAPLIIGLGYGAVTPASSELLARTARPDRLALTFSIKQTGVPGGAALAGA
                     VLPALTVAIGWRPALLSVAACGVVIAALAQSTRRRLDVIGADRPFSLAALMAPLRIVL
                     RDRRLAELSVVGYIYAAMQMCLMSFLVVYLTEALDYPLIAAGLALTTANVGGVAGRIA
                     WGAIADRWIAPRRMLGFIGLATGVCAFAAASFSAAWPHAALLAVAALFGATAIGWNGV
                     QLSQVARHAPPGQAGAITGASGFITFAGVVSGPPLFALLAVVTDGYRTGFVVFGFLCL
                     LCGAALLARSR"
     gene            1808..>2139
                     /locus_tag="VHT22_01105"
     CDS             1808..>2139
                     /locus_tag="VHT22_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019647012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX3136175.1"
                     /translation="MTEHELLLRPLLALVAGGLVGLERSFHGRAAGLRTYALVSFASA
                     LLVGIAQFTDSAPGGAQTNVARVIQGIVTGIGFLGAGVIIKEGFTVRGLTTAASVWVV
                     SAIGVAFGT"
BASE COUNT          387 a          717 c          740 g          295 t
ORIGIN      
        1 cgagaagtcg gacgtccacg tccacgtgcc ggcgggtgcg acgcccaagg acggtccgag
       61 cgctggcgtc gcgatgatcg tcgcacttgt gtcgttgctg accgaccggc cggtgcgcac
      121 ggacgtcgcc atgaccggcg aaatcagcct gcgcggtctg gtgctgccga tcggcggcgt
      181 caaggaaaaa gtactggctg cattgcgcgc aggcattcgc accgtgatgc tgccctcgcg
      241 caaccggcga gacctcgagg agatcccgcc cgaggcgcgc gcccaattgg cgttcgtgtg
      301 gctcgaagaa gtcgacgatg cgattgcggc ggcgctggcg ccgggagagt cgcaagccgt
      361 tcccgtcccg gatccgacct aggcttgcat cccgtcaccg ggatcgcgcc agcagcgcag
      421 caccgcacag caggcacagg aagccaaaga cgacgaagcc ggttcggtag ccgtcggtaa
      481 cgacggcgag cagcgcaaag agcggcgggc ccgagacaac gccggcgaag gtgatgaaac
      541 ccgaggcgcc ggtgatcgcg ccggcctgtc ccggcggagc gtgccttgcc acctgggata
      601 actggacgcc gttccacccg attgcggtag cgccgaaaag cgccgcaacg gcgagcagag
      661 ccgcgtgcgg ccacgccgcg ctgaagcttg ccgccgcgaa cgcgcaaaca ccggtggcga
      721 ggccgatgaa gccgagcatg cggcgcggcg caatccaacg atcggcgatc gcgccccagg
      781 cgatgcggcc ggcgacgccg ccgacattcg ccgtcgtcag ggccagtccc gcagcgatca
      841 gcggatagtc caatgcttcg gtcagataga cgaccagaaa actcatcagg cacatctgca
      901 tcgccgcgta gatgtaacca acgaccgaaa gctcggccag ccggcgatcc cgcagcacga
      961 tgcgcagtgg cgccatcagc gccgcaagcg aaaatggacg gtcggcgccg atgacgtcga
     1021 gccgtcgtcg cgtcgactgt gcgagagcgg cgatgacgac gccgcaggcg gcgacggaca
     1081 gcagcgcagg ccgccagccg atcgcgaccg tcagcgccgg caacacggcg cctgccagcg
     1141 cggcgccgcc gggcacaccg gtctgcttga tcgagaacgt aagcgccagg cggtcaggac
     1201 gagcggtgcg tgcgagcaat tcggatgagg ctggcgtaac ggcgccgtag cccaggccga
     1261 tgatcagtgg cgcgacggcc agcatcgcgg ccgcgcgcga aggcagcagc gcgaccagca
     1321 ggatgccggc ggcgcacagc agcacgcata gctgcgacac gcggatcgct ccgcggcgcg
     1381 cgatgaaatg gccggaggcg agactcgcag tcatagatcc cacgtagagc agtccgacga
     1441 aaatcccgac cagcttggca ggcacgccga agtcgcgcgc gatctccggc gccagcaccg
     1501 caatcgccgc accggacagc gcggtgaaga cctgaatggc cagcgtggcg gacagcgcga
     1561 tagccgcggt ggatgccgac gacggcgcgg cgcgtggggt gttcaagaat cggcagccgg
     1621 ttggtctcgc agtagatgct ttgcccgatt tttcgctacc gctgatccgg aattatagct
     1681 gccaggcttc tgcgcctttt cccgtgccgg aaacaatgct ccggctcgcg ccttcgccga
     1741 tcgcatccgc tcgaaagcgc cgccggcgcc aatcttcgct atgatgtcgc gaccggaggc
     1801 cacgcgaatg accgagcacg aactgttgct gcgccctctg ctcgcgcttg tcgccggcgg
     1861 cctggtggga ttggagcgca gcttccacgg tcgcgccgcg ggcctgcgca cttacgcgct
     1921 ggtgtcgttc gcctcggcac tgctcgtcgg catcgcgcaa ttcacagact cggcgcccgg
     1981 cggcgcccag accaacgtgg cccgtgtgat ccagggcatc gtcacaggaa tcggatttct
     2041 cggcgccggc gtcatcatca aggaggggtt caccgtgcgc ggcctgacga ccgctgcgtc
     2101 ggtctgggtc gtctcggcga tcggcgtggc gttcggcac
//
LOCUS       DATANS010000075         3640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_550289, whole genome shotgun sequence.
ACCESSION   DATANS010000075 DATANS010000000
VERSION     DATANS010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550289"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VHT22_01110"
     CDS             <1..562
                     /locus_tag="VHT22_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX3136176.1"
                     /translation="TLFFEFHGSEAGVREQSATMRAISDEFGGSEFQWATLPEDRTRL
                     WKARHAAYYATLALKPGLTAIATDACVPISRLAECILATKADIEKSGLTVTIVGHVGD
                     GNFHLVVLYQPGDAGQRERADALARAVSMRAIAMGGTCTGEHGIGWHKLDLLAQEHGE
                     AVDLMRSIKRALDPQGIMNPGKTVPF"
     gene            616..1278
                     /locus_tag="VHT22_01115"
     CDS             616..1278
                     /locus_tag="VHT22_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX3136177.1"
                     /translation="MYTLYFGNKNYSSWSLRAWVLLKAFGIPFEEQRITLYEPDSPER
                     IRVHSPSGKVPCLRDGSTIVWDSLSIAEYLAERHPGMWPADDVTRAWARSATAEMHSG
                     YSELRNEFGMNLRIRQTRKPSPAVASDIARIAALWREGRERFGRSGDFLCGAFGIVDA
                     FYCPVAYRFDSYGVVLDGAAAAYHRALLDLPAMREWAEGASRETEHIPMLDSTMPQAQ
                     QQ"
     gene            1295..2398
                     /gene="glcE"
                     /locus_tag="VHT22_01120"
     CDS             1295..2398
                     /gene="glcE"
                     /locus_tag="VHT22_01120"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEX3136178.1"
                     /translation="MSDLVAAWRDRVRAATAATTPLRIVGGGSMDFYGQRLTGEVFDV
                     SGHAGIVDYDPTELVITARCGTRLFDIEQAMAQSGQMLAFEPPHFGPKATIGGSIAAG
                     LSGPRRPYAGAVRDLVLGIRLLDGRGEELAFGGRVMKNVAGFDVSRLIAGSLGTLGVI
                     LEVSLKCLPLPRAETTRIFELSADEALRRLNVWSAAPLPLSASCWHQGRLSVRLSGAD
                     SAVAAAARRLGGEPLDDAVSYWAAVRDQTHDFFAPFRAQNNGDATASSGPALWRLSVR
                     ATAPHTAFGGATMIEWGGALRWLVAQEEGDTEQARAWAAAHGGHATLFRASDKSAGVF
                     QPLPPALLALHQRLKAALDPAGIFNPGRMYAEL"
     gene            2409..>3640
                     /gene="glcF"
                     /locus_tag="VHT22_01125"
     CDS             2409..>3640
                     /gene="glcF"
                     /locus_tag="VHT22_01125"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEX3136179.1"
                     /translation="METHLADFIRDTPDGRDAESILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQMLEGDAVTATTTLHLDRCLSCRSCETTCPSGVSFGRLLDIGHRVA
                     EQKIERPAGERMRRWLLRKGLTHGPWFGLALAAGRIARPLLPRALAARVPASRRAKRW
                     PEPRHARRMLLLAGCVQPALAPSIDAALARALDRVDITLVRASGSGCCGAVSDHLGAH
                     DEALDFARANIDAWWPHVEQGVEAIVVSASGCGVVVKAYGHLLRDDPAYADKARRVAA
                     LARDSVEVVAAEWKRFAPQVAMDHGPQRVAFHSPCSLQHGQRLVGDVEEILEAIGLEL
                     TPVADPHLCCGSAGTYSILQPELAGKLKANKLAALEAGAPDVIATANIGCLVHLASTA
                     TRPVLHWVEIFESRLRERP"
BASE COUNT          573 a         1192 c         1264 g          611 t
ORIGIN      
        1 gacactcttc ttcgagttcc acggcagcga agcgggtgtg cgcgagcaat cggcaaccat
       61 gcgggcgatc agcgacgaat tcggcggcag cgaattccaa tgggcgacgc tgcccgagga
      121 ccgcacgcga ctgtggaagg cgagacacgc cgcgtactac gcgacgcttg cgctcaagcc
      181 cggactgacc gccatcgcca ccgacgcgtg cgtgcccatt tcccgcttgg ccgaatgcat
      241 attggcgacc aaggccgaca tcgaaaaaag cggcctgact gtcaccatcg tcggccacgt
      301 cggcgacggc aattttcatc tggtcgtgct ctaccaaccg ggcgatgccg gccagcgcga
      361 gcgcgccgac gcgctggcgc gagcggtcag catgcgcgcg atcgccatgg gcggcacctg
      421 taccggggag cacggcatcg gctggcacaa gctcgacctg ctggcgcagg agcatggcga
      481 agcggtcgat ctgatgcgct cgatcaagcg ggcgctcgat ccgcaaggaa tcatgaaccc
      541 gggcaagacg gtcccgtttt gacggtgctt cggacgactg gcgagcgctg acggcaaacc
      601 ctcggcgaga actccatgta tacgctctac ttcggcaaca agaattactc gtcctggtcg
      661 ctgcgcgcct gggttttgct gaaagccttc ggcattccgt tcgaggagca gcgaatcacg
      721 ctctacgagc cggattcgcc ggagcggatc cgcgtccatt cgccttcggg caaggtgccg
      781 tgtttgcgcg atggcagcac gatcgtctgg gactcgcttt cgatcgccga gtatctggcc
      841 gagcggcatc ccggcatgtg gcccgctgac gacgtcacgc gcgcctgggc gcgctcggcg
      901 accgccgaga tgcactccgg ctacagcgag ctgcgcaatg aattcgggat gaatctgcgc
      961 atccgacaaa cgcgaaaacc ctcgccggca gtcgccagcg acatcgcgcg gattgccgcg
     1021 ctgtggcgcg aggggcgcga gcgcttcggc cggagcggag atttcctgtg tggcgccttc
     1081 ggcattgtcg acgcgttcta ctgcccggtt gcatatcggt tcgacagtta cggcgttgtg
     1141 ctggacggtg cagcggccgc gtaccatcgg gcgctgctcg acttgccggc gatgcgcgaa
     1201 tgggccgaag gcgcttcgcg cgagaccgag catattccga tgctcgacag cacgatgccg
     1261 caggcgcagc agcaatgact cggccgggcg gccgatgagc gatcttgtcg ccgcgtggcg
     1321 cgatcgcgtg cgcgcggcaa cagcggcgac gacgccgctg cgcatcgtcg gcggcggcag
     1381 tatggacttc tacggacagc gtctgaccgg cgaggtcttc gacgtctcgg ggcacgccgg
     1441 catcgtcgac tatgatccaa cagagctggt gatcaccgcg cgatgcggca cccggctgtt
     1501 cgacatcgag caggcgatgg cgcaatcggg ccagatgctc gcgttcgagc cgccgcattt
     1561 cggaccgaag gcgactatcg gcggctcgat cgccgcgggc ctgtccggac cgcgccgacc
     1621 ctacgcgggc gccgtgcgcg atctggtgct gggcatacgg ctgctcgacg gacgcggcga
     1681 agagctcgct ttcggcggga gggtgatgaa aaacgtcgcc ggcttcgatg tctcgcggct
     1741 gattgccgga tcgctgggga cgctgggcgt catcctcgag gtgtcgctca agtgcctccc
     1801 cctgccgcgc gcggagacga cgcggatctt cgagctgtcc gccgatgaag cgctgcgcag
     1861 gctcaacgtc tggagcgcgg cgccgctgcc cttgtcggcg agctgctggc accagggaag
     1921 gctcagcgtg cgcctgtccg gcgctgacag cgcagtggcg gccgcggcgc ggcgattggg
     1981 cggcgagccg ctcgacgacg ccgtgagcta ctgggccgcg gtgcgcgacc agacgcatga
     2041 tttcttcgcg ccctttcgcg cacagaataa tggcgacgcg acggcgtcga gcgggcccgc
     2101 gctgtggcgt ctgtcggtgc gcgcgaccgc accgcacacg gcgttcggcg gcgcgacgat
     2161 gatcgaatgg ggcggcgcac tgcgctggct tgtcgcgcag gaggagggcg ataccgagca
     2221 ggcccgtgcg tgggccgcgg cgcacggcgg ccatgcgacc ttgtttcgcg cctccgacaa
     2281 gtcggccggc gtgtttcaac cgttgccgcc tgcgctgctc gccttgcatc agcgtctgaa
     2341 agcggcgctg gatccggccg gcattttcaa tccgggtcgg atgtacgcgg aactctgaaa
     2401 ggacctggat ggaaacccat ctggccgact tcattcgcga cacccccgac ggacgcgacg
     2461 cggaatcgat cttgcgcgcc tgcgtgcatt gcggcttctg cacggcgacc tgcccgacct
     2521 accagctatt gggcgacgag ctcgacggcc cacgcggacg catctacctg atcaagcaga
     2581 tgctcgaagg ggatgcggtc accgccacta ccacgcttca cctggaccgt tgtctgagct
     2641 gccgcagttg cgaaacgacc tgcccgtcgg gcgtaagctt cggccggctg ctcgatatcg
     2701 gacaccgcgt cgccgagcaa aaaatcgagc gtccggccgg cgagcgcatg cgccgctggc
     2761 tgctgcgcaa gggcctgacc catggccctt ggttcggttt ggcactcgcc gcgggtcgca
     2821 tcgcgcggcc tttgctgccg cgcgcgctcg ccgccagggt tcccgcgtcg cgccgcgcca
     2881 aacgatggcc ggagccgcgc cacgcccggc ggatgttgtt gcttgcgggc tgcgtgcagc
     2941 ccgcgctggc cccgagcatc gatgccgcgc tcgctcgcgc gctcgaccgg gtcgacatta
     3001 cgctggtccg ggcttcgggt tcgggctgct gcggcgcagt gagcgatcat ctgggtgcgc
     3061 acgacgaagc gctcgatttt gcgcgcgcca acatcgacgc ctggtggcct catgtggagc
     3121 agggcgtcga ggcgatcgtc gtcagcgcaa gcgggtgcgg agtcgtcgtc aaggcgtatg
     3181 ggcatctgct gcgcgacgat ccggcatatg ccgacaaagc gcggcgcgtc gcagcgctgg
     3241 cgcgcgattc ggtcgaagtc gtcgccgccg aatggaaacg cttcgccccg caggtggcga
     3301 tggatcacgg gccccagcgc gtggcgttcc attcgccgtg ctcgttgcag cacggccagc
     3361 gcctggtcgg cgatgtcgag gagatactgg aagcgatcgg gctggaattg acgccggtcg
     3421 ccgatccgca tctttgctgc ggctcggcgg gcacctactc gatcttgcag cccgagctgg
     3481 cgggcaagct caaggccaac aagctggccg cgctggaagc gggtgcgccg gacgtcatcg
     3541 cgaccgccaa catcggttgc ctggttcatc tggccagtac cgcgactcga ccggtgctcc
     3601 attgggtgga gattttcgaa tcgcgactgc gcgagcggcc
//
LOCUS       DATANS010000076         2046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_717461, whole genome shotgun sequence.
ACCESSION   DATANS010000076 DATANS010000000
VERSION     DATANS010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717461"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="VHT22_01130"
     CDS             <1..1352
                     /locus_tag="VHT22_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136180.1"
                     /translation="NEYFADGLSEELLNVLAKIRGLRVASRTSAFFFKGKDIDIPTVA
                     QKLNVATILEGSVRKSGKRVRITAQLIQVATDSHLWSDTYDRELDDIFAVQDDIAQSV
                     VKELRAALLGEGLGAVADAKAKAEVQAAATGRADNPEAYRLYLQGRYFIERMTEVDLA
                     RSVDYFRQAIALDPDFALGWAGLSQAYSIQAGYGWAPVTEGYERARDAAKQALAIAPD
                     LPEGHVRLGQVLESLDWNWKGANDEYQRAVALAPGNADVLRAVANMTRIFVGPDAGIE
                     LLRQAAALDPLSSSAHRMLGLRCFLAGRLDEATAALKTSLDLNPKAGLVHCFLCATNL
                     AQGRPTEALELAEREALPDFRLLGIALAQHALGRKEASDAAMAQLIERHGNTAAYQIA
                     EACTWRGERDRVFEWLERAYTQRDPGLAMTSSDPFFRPVHDDPRWLPFLKKMGFPSR"
     gene            1362..1988
                     /locus_tag="VHT22_01135"
     CDS             1362..1988
                     /locus_tag="VHT22_01135"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3136181.1"
                     /translation="MPDFEKPLETWNARFARDDYLFGEEPNAFLRKHVHFLRPASSVL
                     CVADGEGRNGVWLAEQGHRVTSFDFAPNALAKAKRLAQQRNVSVDQRLGDAYTWPWAP
                     SQYDALVAVFIQFLPPHRRDDAFAGMMSAVKPGGLFLLEGYRPEQVDYRTGGPPKREH
                     MYTREWLQTTFTGWEILVLEDYDAVIHEGQAHDGMSALIDLVARKIDG"
BASE COUNT          358 a          633 c          694 g          361 t
ORIGIN      
        1 agaacgagta tttcgcggat ggcctttcgg aggagttgct caatgtgctg gcgaaaatcc
       61 gcggcctgcg cgtcgcgtcg cgaacgtcgg cattcttttt caaaggcaag gacatcgaca
      121 ttccgacggt cgcgcagaag ctcaacgtcg ccaccatcct cgagggcagc gtccggaagt
      181 ccggcaagcg ggtccgcatc acggcacagc tgatccaggt cgcgaccgac tcgcacctgt
      241 ggtcggacac ctacgaccgc gagctcgatg acatctttgc cgttcaggac gacatcgcgc
      301 agtcggtggt caaggagctg cgggcggcgc tgctgggcga gggtctgggc gcagtcgcgg
      361 atgcgaaggc aaaggccgag gtgcaggcag cggcaactgg ccgcgccgat aatcccgagg
      421 cttatcggct ctaccttcag gggcgctatt tcatagaacg gatgacggag gtcgacctgg
      481 cgcggagcgt cgattacttc cggcaggcga tcgcgctgga cccggatttt gcgctcggtt
      541 gggcggggct gtcgcaggct tactccatcc aggcgggtta cggttgggcg cccgtgacgg
      601 aaggatacga acgggcgcgc gatgccgcca agcaagcgct ggcgatcgcg ccggatctgc
      661 cggaagggca cgtccgtctc ggacaggttc tcgagagcct cgactggaac tggaaaggcg
      721 ccaacgacga gtatcagcgc gcggtcgcac tggcgcccgg gaacgccgac gtactgcgcg
      781 ccgtcgcgaa catgacgcgg atttttgttg gcccggatgc gggcatcgaa ctgttgcgcc
      841 aggccgcggc gctggatccg ctgagttcat cggctcatcg tatgctgggt ttgcgctgct
      901 ttctggcggg ccgcctcgac gaggcgacgg cggcgctgaa gacgtcgctc gatctgaatc
      961 ccaaggcggg cctggtccat tgttttctct gcgccacgaa cctcgcgcaa ggccggccga
     1021 ccgaagcgct cgagctggct gagcgcgagg cgctgccgga ttttcggttg ctggggatcg
     1081 ctctcgcgca acatgcgctc ggccgcaagg aagcctcgga cgccgcgatg gcgcaattga
     1141 tcgaacgcca tggcaacacc gccgcctacc agatcgcgga ggcttgcacc tggcgcggtg
     1201 aacgcgaccg cgtgttcgaa tggctggagc gggcctacac gcagcgcgat cccggcttgg
     1261 cgatgacttc gtcggatccg ttcttccggc cagtgcacga cgacccgcgg tggttgcctt
     1321 tcctgaagaa aatgggattc cccagtcgtt gactgagaac catgcccgac ttcgaaaaac
     1381 cgctcgaaac ctggaacgca cgcttcgcgc gcgacgatta cctgttcggc gaggagccga
     1441 acgcgttcct gcgcaagcac gtgcattttc tccgaccggc gagctcggtg ttgtgcgttg
     1501 ccgacggcga aggacgcaac ggcgtctggc tcgccgagca gggtcatcgc gtcacctcgt
     1561 tcgacttcgc gccgaatgcg ctggccaagg cgaagcgcct ggcgcagcag cgcaatgtca
     1621 gcgtcgatca gcggctcgga gatgcctata cctggccctg ggcccccagc cagtacgacg
     1681 cgctggtcgc ggtgttcatc cagtttctgc cgccgcaccg gcgcgatgac gctttcgccg
     1741 gaatgatgtc cgcggtaaag cccggcggtc tgttcctgct cgaaggctac cggcccgagc
     1801 aggtcgacta tcgcaccggc gggccgccga agcgtgagca catgtacacc cgcgagtggc
     1861 tgcaaactac gttcaccggc tgggagatcc tggtcctcga ggattacgat gcggtgattc
     1921 acgaagggca ggcgcacgac gggatgtcgg cgctgatcga ccttgtggcg agaaaaatag
     1981 acggctgagc gcgggcgggc gctacgccgg ttccagggtc tcagatctcg acgcgcgtgc
     2041 ccaatt
//
LOCUS       DATANS010000077         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_172464, whole genome shotgun sequence.
ACCESSION   DATANS010000077 DATANS010000000
VERSION     DATANS010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172464"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /gene="gltX"
                     /locus_tag="VHT22_01140"
     CDS             <1..1343
                     /gene="gltX"
                     /locus_tag="VHT22_01140"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX3136182.1"
                     /translation="ALYPWAYARKHGGVVVLRIEDTDAERSTQEAVQAILDSMAWLGL
                     DYEEGPIYQSQRMDRYAEVVAAMLAGGTAYHCYATADELDALRAAQLARGEKPRYDRR
                     WRPEPGKILPASPAGIPPVVRFRNPLGGVVEWQDAVKGRIEIGNDELDDLIIARSDGA
                     PTYNFCVVVDDMDMRITHVIRGDDHVNNTPRQINMIRALGGTEPVYAHLPTVLTPEGD
                     KLSKRHGARGVLQYRDDGYLPEAVVNYLARLGWAHGDAEIFSRREFVDWFDLAHLSSA
                     PGRFDPEKLKWVNHEHIKHLPADELGRRLIPFLERAGLDVAAGPAPASVAELLRDRMA
                     TLAEMADAAHYFYADPTHATNVPAKFAEVMTDPVRSALPELRAAFESVEWSRDAIGAA
                     IKAVAAKRGLKTSQLMMAARALVTGEPQTPPIDAVLALLGREHTIDRLHRGLTG"
     gene            1407..>1584
                     /gene="pyrG"
                     /locus_tag="VHT22_01145"
     CDS             1407..>1584
                     /gene="pyrG"
                     /locus_tag="VHT22_01145"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001076.1"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HEX3136183.1"
                     /translation="MTKFVFVTGGVVSSLGKGIAAASLAAILTSRGIRVTNLKLDPYI
                     NVDPGTMSPFQHGEV"
BASE COUNT          271 a          530 c          516 g          267 t
ORIGIN      
        1 cggctttgta cccgtgggct tacgcgcgca agcacggcgg cgtcgttgtg ctgcgcatcg
       61 aggataccga cgccgagcgc tcgacgcagg aagcggtgca ggcgattctc gacagtatgg
      121 cgtggcttgg cctcgactac gaggaaggcc cgatctatca atcgcagcgg atggatcgct
      181 atgcggaagt ggtcgccgcg atgctcgctg gcggcaccgc ttatcactgc tacgcgacgg
      241 cggacgagct cgacgcgctt cgcgcggcgc agcttgcgcg cggtgaaaag ccgcgctacg
      301 accggcgctg gcgcccggag cccggcaaga ttctgcctgc atctccggcc ggaatcccgc
      361 cggtcgttcg cttccgcaac cctttgggcg gcgtcgtcga atggcaggat gcggtcaagg
      421 gccggatcga aatcggcaac gacgagctcg acgacctgat catcgcccgg tccgatggcg
      481 caccgaccta caatttctgc gttgtcgtcg acgacatgga catgcgcatc acgcacgtga
      541 tccgcggcga cgaccacgtg aacaacacgc cgcgccagat caatatgatc cgcgcgcttg
      601 gcggcaccga gccggtgtac gcgcacctgc cgaccgtgct gacgccggaa ggcgacaaac
      661 tgtctaagcg gcacggcgcg cgcggggtgc tgcagtaccg cgacgacggc tatctgcccg
      721 aggcggtcgt caactacctg gcgcgcctgg gctgggcgca cggcgacgcg gagatattct
      781 cgcgccggga attcgtcgac tggtttgacc tggcgcacct ttcgtccgca ccggggcggt
      841 tcgatccgga aaagctcaag tgggtcaatc acgagcacat caagcatctg ccggcggacg
      901 agctcggccg gcggctcatc ccgtttctcg aacgtgccgg gctcgacgtc gcagccgggc
      961 cggcgcccgc cagcgtcgcc gagctgttgc gcgaccgcat ggccaccctg gcggaaatgg
     1021 ccgatgccgc gcactacttc tacgcagacc cgacgcacgc gacaaacgtc ccggccaagt
     1081 tcgcggaggt aatgaccgat cccgtccgat ccgccttgcc cgaactgcgg gccgcgttcg
     1141 agagcgtcga gtggagtcgt gatgcgattg gcgccgcgat aaaggcggtc gctgcaaagc
     1201 gtggcctcaa aacctcgcag ctgatgatgg cggcacgggc tctcgtcacc ggcgagccgc
     1261 aaaccccgcc gatcgacgcc gtgctcgcgc tgctcggtcg cgagcacacg atcgaccgct
     1321 tgcaccgagg gcttaccggc taggcgcgat cgtttgtgcg cgacgaccgg ttcgggtaaa
     1381 atgggaattt cctgaacttg tcgcccatga ccaagttcgt tttcgtgact ggcggcgtcg
     1441 tttcctctct ggggaagggc atcgccgccg cgtctctggc cgcgattctc acctcccgcg
     1501 gcatccgcgt caccaatctc aagctcgatc cgtacatcaa cgtcgacccg ggcacgatga
     1561 gccccttcca gcacggcgag gtgt
//
LOCUS       DATANS010000078         7658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_255978, whole genome shotgun sequence.
ACCESSION   DATANS010000078 DATANS010000000
VERSION     DATANS010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7658
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255978"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VHT22_01150"
     CDS             complement(<1..857)
                     /locus_tag="VHT22_01150"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEX3136184.1"
                     /translation="MRALSSFATIAVVLSLAAPRADAAIAGALDYDGARLLLNRTGFG
                     ATQAEIEHYVGMSREQAAKALLATSRTAAVTSPPPAMIATGPLQYPRRGAGASDEEIK
                     MFRQQQVREGLDLRGWWVGEMLSTPSPLTERMTLFWHNHFVSSQQKVKLTELMYRQNV
                     TLRAQALGNFGAMLHAVARDPAMIIYLDSAQNRKGAPNENFARELMELFTLGEGNYAE
                     SDVKEAARAFTGWSLDRETGEYTYRRVWHDYGVKSVLGRTGRFDGDDVIDVLLARPET
                     ASFIAAKVWR"
     gene            complement(969..1841)
                     /locus_tag="VHT22_01155"
     CDS             complement(969..1841)
                     /locus_tag="VHT22_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX3136185.1"
                     /translation="MNDYRFRILNVFAREDALSGNPLAVFEEAGSLDETTMQALALQF
                     NLSETTFILPSTVATARVRIFTPSFEMPFAGHPTLGTAHVVRDCLRGGDSLTLEMRAG
                     IVPVTARADRWTLEAKAPRTREVAAPRDVLAAMLGLTTADLGTTPLWVDTGSEQLVIP
                     LASAEAVARCAPDPALLLRHASLDLPGPPGSARSVRAMAYVWSRVGEGSVSTRFFFSK
                     QGAVVEDPGTGSACANLGGWLVATGAARPQSLTVAQGEAVGRPCRLGLEVDADGRIFV
                     SGRVLELGRGTIRL"
     gene            complement(1841..2929)
                     /gene="mnmH"
                     /locus_tag="VHT22_01160"
     CDS             complement(1841..2929)
                     /gene="mnmH"
                     /locus_tag="VHT22_01160"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEX3136186.1"
                     /translation="MSAVLYPGSRVDVDALPAHPDRIDVRSPAEFAEDHIPGARNHPV
                     LDDDERARIGTMHAASPFEARRLGAALVARNIAKMLETVFAEQPREWKPLVYCWRGGQ
                     RSRAVAHVLNEVGWRAVQLDGGYRAYRRHVVARLAVQPARYRFRVVCALTGSGKSQLL
                     GALADAGAQTLDLEGIAQHRGSLLGQVPGTPQPSQKKFETDLLAALGLLDPRRPVYVE
                     AESRRIGAIQLPDALLQRMHDGETIILRTPLSERIALLKSEYRHFLQSPALLLDRLRP
                     LVEFHGKAAFARWETLAASGDWDALIGELLESHYDPLYGRSMARHFPAAGPSAVFGLR
                     DPSPAGFAALAADVLAASDDSRMAVMER"
     gene            complement(2926..3510)
                     /locus_tag="VHT22_01165"
     CDS             complement(2926..3510)
                     /locus_tag="VHT22_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEX3136187.1"
                     /translation="MPASAQRASPVILLVGDSVSAGYGLPTGVGWATLLQQRLDAQHL
                     RYRVVNASISGDTTAGGRARLGALLAQHHPAITIIELGGNDGLRGGSLDAMRDNLDAM
                     TSAAQNAGSRVLLVGIRVPPNYGAAYGERFAATFGEVARARKAALVPFLFEGFAENDS
                     MFQPDRIHPLAAAQAKMLDNVWPSLAPLLSAKAA"
     gene            3757..4464
                     /locus_tag="VHT22_01170"
     CDS             3757..4464
                     /locus_tag="VHT22_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3136188.1"
                     /translation="MADPVIRAVALSKLVISGEAPLKILDDVSFDIAAGESVAIVGAS
                     GSGKTTLLGLLAGLDRPTTGEVWLGNTALNGLSEDARAALRQRWVGFVFQSFQLLPAL
                     TALENVMLPLELAGANDAGARAREWLARVGLAARVTHYPKQLSGGEQQRVAIARAFSG
                     EPQVLMADEPTGNLDGATGTEVAELMFRLNRERSTTLLLVTHEVTLAARCARRLSLAT
                     GRLVGDEQSSLGGATAA"
     gene            4461..6953
                     /locus_tag="VHT22_01175"
     CDS             4461..6953
                     /locus_tag="VHT22_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX3136189.1"
                     /translation="MKTPDTVRLAWRLLVRDWRAGELRVLIAALVLAVASVGTVGFFT
                     DRVKGALTRQANLLLGADLLLSGDRPLPDEFMREARSRGLAAVPAIKFNSMVQAAGGA
                     GAAVLADVKAVTAGYPLRGAILLVDPANAAGKVAPGVPPPGEVWIDSRLAARLGVQQG
                     AKLAVGESTLTVGAIVQQDPEVTSGLLALGPRLMMNLDDVAATRLLQPGNRAAYRLLV
                     AGDAINAFRDWAQAHLDRGQRLESIRDLRPEVRQTLERAEQYLGLAALVAVMLAAVAI
                     ALAASRYLRRHLDAAAMMRCLGAPQRQILSLFALQFMVLGVGASIAGCALALGGQQLL
                     IVLLGGIVSADLPPPSAWPAFAAAASGLAVLFGFALPPLFALSRVPPLRVLRRDLGLP
                     RAGGWLAYALGAATIALLIGWQAQDATTGAIMIGGTAALLAVAGAVAWALIALLRLLP
                     QRGYSWRYGLANLRRRPLASSLQIGALGLGLMALLLLTLVRGDLLRNWRASLPADAPN
                     VFLINILPDQIEGMHALLERELKADVPLYPMVRGRLVAVNDVPVDSTRLTDARARRLA
                     EREFNLSWMNELPRSNRVVAGQWWKAGDAGVMSLEDGIAETLGVKLGDVLTYDIVGTR
                     VSARVTNLRKVDWDSFRVNFFALYPPGMLDGLPTTYIAATRAPDNPAWLNGLLAQYPN
                     VLAIDVGELLHQVGAIVEQVARAVEFVFLFTLAGGVLVLEAAIAATQDERLYDAAVLR
                     TLGASRRQLTVAQLAEFLTLGTLAGLVAAGGATATAYVLADRVFHIPFGWNPWLWVIG
                     IVGGALGVAAAGWIGTRRTLRQPPLALLRQFG"
     gene            7082..>7658
                     /locus_tag="VHT22_01180"
     CDS             7082..>7658
                     /locus_tag="VHT22_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX3136190.1"
                     /translation="MGNALFIIWRESAEAMLVVGILYAWLRKHPDVKRGMRYLWAGVG
                     TGVALALALAAVMLGIARSLSDQGLEYFQTAMMLIAAGLIVQMVFWMRRHGRTFKREL
                     EQDMQKNASAANWWGLLLVVALAVGRESAETVVFLYGLGTQYASFWSFIGILVLGLGA
                     ALATFWVLQQGGRIVSWRTFFRVSEILLLLLA"
BASE COUNT         1179 a         2556 c         2663 g         1260 t
ORIGIN      
        1 cgccagacct tggcggcgat gaagctcgcc gtctcgggtc gcgcgagcag gacgtcgatc
       61 acgtcgtcgc cgtcgaagcg gccggtgcgg cccagcaccg acttcacgcc atagtcgtgc
      121 cacacgcgcc gataggtgta ctcgccggtc tcgcggtcga ggctccagcc ggtgaacgcg
      181 cgcgccgctt ccttgacgtc gctctcggcg tagttgcctt cgcccagcgt gaagagctcc
      241 atcaattcgc gcgcgaaatt ctcgttcggc gcgcccttgc gattctgtgc gctgtccagg
      301 tagatgatca tcgccggatc gcgcgcgacc gcgtgcagca tcgcgccgaa gttgccgagc
      361 gcttgcgcgc gcagcgtgac attctgccga tacatcagct cggtgagttt caccttttgc
      421 tggctggaca cgaaatggtt gtgccagaac agcgtcatcc gctcggtcag cggcgatggc
      481 gtcgacagca tttccccgac ccaccagccg cgcagatcca ggccttcgcg gacctgctgc
      541 tggcggaaca tcttgatctc ctcgtcagac gcgcctgccc cgcggcgcgg atattgcagc
      601 ggtccggtcg cgatcatcgc cggcggcggc gatgttaccg ccgcggtgcg cgaggtcgcg
      661 agcagcgctt ttgcggcctg ctcgcgcgac atgccgacgt agtgttcgat ctcggcttgc
      721 gtggcgccga agcccgtgcg gttgagcaaa agcctcgcgc cgtcgtaatc gagcgcgccg
      781 gcgatcgccg catcagcccg tggtgcggcc agcgacagaa cgactgcgat cgtggcaaac
      841 gaggagaggg cgcgcatgcg tggccgaggc ggtccgatcg gcaatcaacg gctcagtgta
      901 gcgagctgtt gactttgcgt gcgccttcgg ttgtaacagc ctgttgcgct tcgctggcga
      961 gagaggggtc agagcctgat cgtgccgcga ccgagctcca gcacacgccc cgagacgaaa
     1021 atgcgcccat cggcgtcgac ctcgaggccg agccggcacg ggcgtcctac ggcttcgccc
     1081 tgggcgacag tcaatgactg cggccgcgcg gcgcccgtcg ccaccagcca cccgcccaga
     1141 ttcgcgcaag cggagcctgt gcccggatcc tcgaccaccg cgccctgctt ggaaaagaaa
     1201 aagcgcgtcg agaccgagcc ctcgccaacc cgcgaccaga cgtaggccat cgcgcgcacg
     1261 ctgcgtgcac tgccgggcgg cccgggcaga tcaaggcttg catggcgaag aagcaacgcc
     1321 ggatccgggg cgcagcgggc cactgcctcg gcgctggcca gcggaatcac cagctgctcg
     1381 ctgccggtat cgacccacag cggcgtggtg ccgagatcgg ccgtggtgag cccgagcatc
     1441 gcggccagca catcgcgcgg cgccgcgacc tcacgcgttc gcggtgcctt ggcttcgagc
     1501 gtccagcgat cggcgcgcgc ggtgaccggc acgataccgg cgcgcatctc cagcgtcagc
     1561 gaatcgccgc cgcgcaggca atcacggacg acgtgcgcgg tcccgagcgt cggatgcccg
     1621 gcgaatggca tctcgaacga tggggtaaag atccgaacgc gcgcggttgc gacggtcgac
     1681 ggcaggatga acgtcgtctc ggagagattg aactgcagcg ccagcgcctg catcgtcgtc
     1741 tcgtcgagac ttccggcctc ttcgaacacc gcgagcggat tcccgctcaa cgcgtcctcg
     1801 cgggcgaaca cattcagaat tcgaaaccgg taatcgttca tcatcgctcc atcaccgcca
     1861 tcctggagtc gtcgcttgcg gcgagcacat cggcagccag cgcggcaaag cccgccggcg
     1921 agggatcgcg caagccgaac acggcactcg gacccgcggc cgggaaatgc cgggccatcg
     1981 agcgtccata tagcggatcg taatgcgatt cgagcagctc cccgatcagc gcgtcccagt
     2041 ccccgctcgc ggcgagcgtt tcccaacggg caaatgccgc cttgccgtgg aattcgacca
     2101 gcgggcgcag gcggtcgagc agcagcgccg gactctgcag gaagtgacgg tactcggact
     2161 tgagcagcgc gatgcgttcc gacagcggcg tgcgcaggat gatcgtctcg ccgtcgtgca
     2221 tccgctggag caacgcatcg ggcagctgga tcgcaccgat ccgccggctc tcggcctcga
     2281 cgtagaccgg gcgccggggg tcgaggaggc cgagtgcggc caggagatcg gtttcgaatt
     2341 tcttctgcga gggttgtggc gtgcccggaa cctggcccag cagcgagccg cgatgctgtg
     2401 cgatgccctc cagatcgagc gtttgcgcgc ctgcgtcggc cagcgccccg agaagctgac
     2461 tcttgccgga accggtcaag gcgcatacca cccggaagcg gtaccgtgcc ggctgcacgg
     2521 cgagccgcgc gacgacatgg cggcggtacg cgcggtaacc gccgtcgagc tgtacggccc
     2581 gccatccgac ctcgttcagc acatgcgcga ccgcgcggct gcgctgtccg ccgcgccagc
     2641 aatagaccag cggcttccat tcgcgcggct gctcggcgaa tacggtctcc agcattttcg
     2701 cgatgttgcg cgcgaccagc gccgcaccga gcctgcgcgc ctcgaacggc gaggctgcgt
     2761 gcatggtgcc gatccgggca cgctcgtcgt cgtcgaggac cggatgattg cgcgccccgg
     2821 gaatatggtc ctcggcgaat tcggccgggc tacggacgtc gatgcgatcg gggtgcgcgg
     2881 gcaatgcgtc gacgtcaacg cggctgcccg ggtacagtac ggcgctcatg cggctttggc
     2941 ggaaagcagc ggggcgagcg acggccagac attgtccagc atctttgcct gcgctgcggc
     3001 cagcggatgg atgcgatcgg gctggaacat cgagtcgttt tcggcgaatc cctcgaacag
     3061 gaacggcacc agcgcagcct tgcgcgcgcg ggcaacctcg ccgaaggtcg cggcgaagcg
     3121 ctcgccgtaa gccgcgccgt aattcggcgg aacgcggatg ccgaccagca gcacgcgcga
     3181 gccggcgttc tgcgccgcgg aagtcattgc gtcgagattg tcgcgcatcg cgtcgaggct
     3241 gccgccgcgc aatccgtcgt tgccgccgag ctcgatgatc gtgatcgccg gatgatgctg
     3301 cgccagcagc gcgcccagtc gcgcgcgacc gccggcggta gtgtcgccgc tgatgcttgc
     3361 attgacgacg cgataacgca gatgctgcgc gtccagccgc tgctgcaaga gcgttgccca
     3421 gccgacgcca gtcggcaatc cgtaaccggc cgagacgctg tcgccgacca gcaggatgac
     3481 cggcgacgcg cgctgtgccg atgcgggcat ggctgcggcg agcagcgccc accacgccac
     3541 gatggcgagt gtacgcacga acatcgagtg gaaacgggga gtcacgacgc attgtagagc
     3601 caggggcggg cggcgccaaa gggcgctgag gcggcaaggt agaatgccgg caacgaccga
     3661 tgaatccgac gcgcaacgct tacgttcttc cgccttccac gttcacgatc gaagcgttga
     3721 caaaacgcgc gccgccgaag cggatgaacc gggccgatgg ctgatccggt catccgcgcc
     3781 gtagcgctgt ccaaactagt gatcagcggc gaggcgccgc tcaagatcct ggacgacgtg
     3841 agcttcgaca tcgccgccgg cgaaagcgtt gcgatcgtcg gcgcatcggg gtcgggcaag
     3901 acgacgctgc tgggattgct tgcggggctc gaccggccga cgacgggcga agtctggctc
     3961 ggcaacacgg cgctgaacgg cctgtccgaa gacgcgcgcg cggcgctgcg ccagcgctgg
     4021 gtcggctttg tctttcagtc gttccagttg ctgcccgcgc tgaccgcgct ggaaaacgtc
     4081 atgctgccgc tggagctggc cggcgcgaac gacgcgggcg cgcgggcgcg tgagtggctc
     4141 gcccgggtcg ggctcgccgc ccgtgttacg cactatccga agcagctctc gggcggcgag
     4201 cagcagcgcg tggcgatcgc gcgcgcgttt tccggcgagc cgcaggtgct gatggccgac
     4261 gagccgaccg gtaacctgga cggcgcgacc ggcaccgaag tcgccgagct gatgttccgc
     4321 ctgaatcgcg agcgcagtac gactttgctg ctggtcacgc acgaagtaac tctcgctgcg
     4381 cgctgcgcgc ggcgattgtc gctggccacc ggacgcctgg ttggcgacga gcagagcagc
     4441 ctcggcggag caaccgccgc atgaagacgc ccgacaccgt gcggctggcc tggcggctcc
     4501 tggtgcgcga ctggcgcgcc ggcgaactgc gcgtcctgat cgccgcgctg gtcctcgcgg
     4561 tggcgagtgt cggcaccgtg ggctttttca ccgatcgagt caaaggtgcg ctgacgcggc
     4621 aggcgaacct gctgctcggc gccgacctgt tgctttccgg cgaccgtccg ttgcccgacg
     4681 agttcatgcg cgaggcgcga tcgcgcgggc tcgcggcggt gccggcgatc aagttcaaca
     4741 gcatggtgca agcggcagga ggtgcgggcg ctgcggtgct ggccgatgtc aaggccgtca
     4801 ccgccggcta tccgctgcgc ggcgcgatcc tgctggtcga tccggcgaat gcggcgggca
     4861 aagttgcgcc aggcgtgccg ccgccgggcg aagtctggat cgacagccgg cttgcggcgc
     4921 gtcttggcgt gcagcaaggc gcgaagctcg ccgtcggcga atcgacgctg accgtcggag
     4981 caatcgtgca gcaggacccc gaggtgacca gcggcctgct cgcgctggga ccccgcctga
     5041 tgatgaacct ggacgacgtc gcggcaacgc gactgttgca gccaggcaat cgcgccgcgt
     5101 accggctgct ggttgccggc gacgcgatca atgccttccg cgactgggcg caggcgcatc
     5161 tcgaccgcgg acagcggctg gagtcgatcc gcgacttgcg gcccgaagtg cggcagacgc
     5221 tcgagcgcgc tgagcagtac ctgggcctcg cggcactggt cgcggtcatg ctcgccgcgg
     5281 tcgccatcgc gctcgccgca tcgcgttatc tacggcgtca tctcgacgct gccgcgatga
     5341 tgcgctgcct cggcgcgccg caacggcaga tcctgtcgct gttcgcgctg caattcatgg
     5401 tgctgggcgt cggcgcgagc attgccggct gcgcgctggc gctcggaggt cagcagctgc
     5461 tgatcgttct cctgggcggc atcgtcagcg ccgacctgcc gccgccgagc gcgtggccgg
     5521 cattcgccgc cgcggcgagc ggtctcgccg tgctcttcgg cttcgccctg ccgccgctgt
     5581 tcgcgctctc gagagtcccc ccgctgcgcg tgctgcgtcg cgacttgggg cttccccgcg
     5641 ccggaggctg gctcgcctac gcgctgggcg cggcgacgat cgcgctgctg atcggttggc
     5701 aggcgcagga cgcgaccacc ggcgcgatca tgatcggcgg cacggccgcg ctgctcgcgg
     5761 ttgccggcgc ggtcgcgtgg gcgcttatcg cacttctgcg tttgttgccg caacgcgggt
     5821 acagctggcg gtacggcctg gccaatctgc gccgccgacc cctggcttcg agtttgcaga
     5881 tcggcgcgct cggactgggg ctgatggcgc tcttgctgct gacgctggtt cgcggcgact
     5941 tgctgcgcaa ctggcgcgcg agcctgccgg ccgatgcgcc caatgtgttc ctgatcaaca
     6001 tcctgcccga ccagatcgaa ggcatgcacg cgctgctcga gcgagagctc aaagccgacg
     6061 ttccgctcta tccgatggtg cgtggtcgtc tggtcgcggt caacgacgtg cccgtcgatt
     6121 cgacccgcct gaccgacgcg cgtgcacgcc ggctcgccga gcgcgaattc aacctgtcgt
     6181 ggatgaacga gctgccgcgc agcaatcgcg ttgtcgccgg gcaatggtgg aaagcgggcg
     6241 atgccggcgt catgtcgctc gaggatggca tcgccgagac gctgggcgtg aagctgggcg
     6301 acgtgctcac ctacgacatc gtcggcacgc gcgtcagcgc gcgcgtgacg aacctgcgca
     6361 aggtcgactg ggacagcttt cgcgtcaatt tcttcgcact ctatccgccg ggcatgctcg
     6421 acggtctgcc gacgacctac atcgccgcaa cccgtgcgcc ggacaacccc gcgtggctca
     6481 acggactgct ggcgcagtat ccgaatgtgc tcgcgatcga cgtcggcgag ctgctgcatc
     6541 aggtcggcgc catcgtcgag caggtcgcgc gcgccgtgga attcgtgttt ctgttcacgc
     6601 tggccggcgg cgtgctggtg ctcgaagcgg cgatcgcggc gacgcaggac gagcgtctct
     6661 acgacgcggc cgtgctgcgc accctcggtg caagccgtcg gcagctgacc gtggcgcagc
     6721 tcgccgaatt tctgacgctg gggacgttgg cgggcctggt cgcggcgggc ggggcgacgg
     6781 ccaccgccta cgtgctggcc gatcgggtct ttcacatccc gttcggctgg aacccgtggt
     6841 tatgggtgat cggcatcgtc ggcggcgcgc tgggcgtggc cgccgccggc tggatcggta
     6901 ccaggcgcac cctgcggcag ccgccgctcg ccctgctgcg ccagttcggc tagcggccgt
     6961 aggccaaacc cacgcggaac caataggcgg ccgcagcttt caaaatgaga acgattcgcg
     7021 ttatcatacg cgctcgattc tagtcagcca ttcgatctta atccgatgct gcgcaacgat
     7081 catgggtaat gcccttttca tcatctggcg cgaatcggcc gaggcgatgc tggtcgtcgg
     7141 catcctttac gcgtggctgc gcaagcatcc ggacgtcaag cgcgggatgc gctatctgtg
     7201 ggcaggcgta ggaaccgggg tcgcgctggc gctggcgctc gccgcggtga tgctgggcat
     7261 tgccaggagc ttgtccgacc agggcctcga atacttccag acagcgatga tgctgatcgc
     7321 cgccgggctg atcgtgcaga tggtcttctg gatgcgccgg cacggccgga cgttcaagcg
     7381 cgagctcgag caggacatgc agaaaaacgc gtcggcggcg aactggtggg gcctgctgct
     7441 cgtcgtcgcg ctggcggtcg gacgcgaaag cgccgagacc gtcgtattcc tctacggcct
     7501 gggcacgcaa tacgcgagct tctggagctt tatagggatc ctcgtgctgg gcctgggcgc
     7561 tgcgctggcg acgttctggg tattgcagca aggcggcagg atcgtgtcgt ggcgcacatt
     7621 ctttcgcgtc agcgagatcc tgctgctgct gctcgccg
//
LOCUS       DATANS010000079         2759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_382033, whole genome shotgun sequence.
ACCESSION   DATANS010000079 DATANS010000000
VERSION     DATANS010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382033"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VHT22_01185"
     CDS             <1..211
                     /locus_tag="VHT22_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136191.1"
                     /translation="VINKARQQRELLRRQRRRSRSKYHYAPETISSRSDMRSTDAPVT
                     MAENIRKRERAPKDEGPDKPPSRKT"
     gene            602..1432
                     /locus_tag="VHT22_01190"
     CDS             602..1432
                     /locus_tag="VHT22_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX3136192.1"
                     /translation="MFATAVIVFRETLEAALIVSIVLAATRGVAGRRRWIGGGVAAGI
                     AGALVIAALTEAIAGALQGIGQELFNAAILIAAVVMLAWHHLWMAAHGRELASRLRRL
                     SDSVREGGATLAALSFAVTLAVLREGAETVLFIQSVATSEPLRNVLAGLALGVTGGAA
                     LGALLYLGLVHVPVRHFFRVTGWMIVALAAGLAAQAARFLEQANVLPPIVPELWDTSP
                     ILSEASLPGQLLHVLVGYTPAPSGLQFMCYVATIATLIIAARWRSTMTGGRPRPAQAT
                     "
     gene            1473..1793
                     /locus_tag="VHT22_01195"
     CDS             1473..1793
                     /locus_tag="VHT22_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX3136193.1"
                     /translation="MRAFLALCCFAVAGAAFADEPTFEIAIRDHRFVPNEITVPAGQK
                     VKLIVKNEDATPEEFESKRLNREKVIPGRSQATIYIGPLRPGEYPFVGEFNESTATGK
                     IVVK"
     gene            1917..2630
                     /locus_tag="VHT22_01200"
     CDS             1917..2630
                     /locus_tag="VHT22_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136194.1"
                     /translation="MKWLSATAFGASVFVSLFNVAPAQAVEPDTIYSPRVEQGEWELE
                     YKGRYFYDRDSEEHRQVQSKFALGYGPTSWWWTELYAEYEHTPGSGGGWSAFEWENRF
                     QFTEPGQSWVDVGALVELDRVHPGTGKEVRAGILLEKEIDETTLTFNWLLNRAFADEE
                     SSHWEQVYRVQWAWRLRPEIQPLVQWQSDEHSAYAGPGIKGKLRIAGQHVEYHLTWLH
                     RISGDIPKDAVRVQLEYEF"
BASE COUNT          496 a          871 c          920 g          472 t
ORIGIN      
        1 cgtcatcaac aaggcgcggc agcagcgcga attgctgcga cggcagcggc gccgctcccg
       61 cagcaagtat cactacgcgc cggaaaccat cagctcacgg tcggacatgc gttcgaccga
      121 cgccccggtg acgatggccg agaatatccg caagcgcgaa cgcgcgccga aggacgaggg
      181 cccggacaag ccgccaagcc gcaagaccta gcgacgccgg ccggccaact ttgccggtgg
      241 ccgtcactct gtcgcccggg ggggccggac ccatcatggg cggcccgagg cgagcggtaa
      301 gcgagccgag ccgggccggc gcgcgaaaac cgatgtattt gcaggaagat gggtgcggca
      361 gtgccgcggg gagggtgcca gatcggggcg gcgccggcga ggagaggtgg tcacggagaa
      421 cgccgccccg tctgggtaaa gcctactaag caatcgacgt gccgtctggg gtatgaccgg
      481 tgtgcaagcc gcttcgtcgg gtcctccagc tattgatacc cgaacctgtt gacagcccga
      541 tcgccaataa tcataatgcg aatcattcgc attatgatca gtcgatcgaa gggctccgac
      601 catgttcgcc acggcagtca tcgtcttccg ggaaacgctt gaggcggcgc tgatcgtttc
      661 gatcgtcctg gcggcgacgc gcggtgtcgc cggccgacgg cgctggatcg gcggaggcgt
      721 cgccgccggc atcgcggggg cgctggtcat cgcggctctg accgaggcca tcgccggcgc
      781 gctgcagggg atcggccagg agctgttcaa cgccgcgata ttgatcgcgg cggtcgtcat
      841 gctggcctgg catcatctct ggatggccgc gcacggccgc gagcttgctt cgcgcctgcg
      901 gcgcttgagc gattcggtgc gcgagggcgg agcaacgctc gccgcgctgt cgttcgcggt
      961 cactctggcc gtgctccgtg agggcgccga aacggtattg ttcattcaga gcgttgccac
     1021 cagcgaaccg ttgcgcaacg tcctggcagg actggcgctt ggcgtgaccg gcggtgcggc
     1081 gctcggcgcg ctgctctatc tcggcttggt gcacgttccg gtcaggcatt tcttccgggt
     1141 caccggatgg atgatcgtgg ccctggccgc gggcttggcc gcgcaggcgg cgcgcttcct
     1201 cgaacaagcc aacgtgttgc cgccgatcgt gcccgagcta tgggatacat cgccgatcct
     1261 gtcggaggcg agcctgccgg ggcagctgct gcacgttctc gttggctaca cgcccgcacc
     1321 ttcgggcctc cagttcatgt gttacgtcgc cactatcgcc actctgatca tcgccgcacg
     1381 ctggcgcagc acgatgaccg gcggccgccc tcggccggcg caggccacct gatctaccca
     1441 tcgcttctca ttccatcgtt cggagctccg atatgcgcgc cttcctcgcc ctgtgctgct
     1501 tcgccgtcgc cggcgccgcg tttgccgacg aaccgacgtt cgagatcgcc attcgcgacc
     1561 accgcttcgt gcccaacgag atcaccgtgc cggcagggca gaaggtcaag ctgatcgtca
     1621 agaacgagga tgcgaccccc gaggaattcg agagcaagcg gctaaaccgc gagaaggtga
     1681 ttccgggccg aagccaggcg accatttata tcggtccatt gaggccgggc gaatatccat
     1741 tcgtcggcga gttcaacgag agcaccgcga ccggcaagat cgtcgtgaag tgaaccaagg
     1801 cggcgcggtc gaatcgcgcc gctctatcgt cgtctccccc atgcgtttga atcgctgcca
     1861 tcgcggcggt tggggaaact tgcgcttttc tgcaccggaa accaacgagg aatgaaatga
     1921 aatggctcag tgccaccgcg ttcggtgcat cggtcttcgt ttcgctgttc aacgtcgcac
     1981 cggcgcaagc cgtcgaaccg gacacgatct actcgcctcg cgtcgagcag ggcgaatggg
     2041 agctcgagta caagggacga tatttctatg atcgcgacag cgaggagcat cggcaggtcc
     2101 agagcaagtt cgcgctgggc tacgggccga cctcgtggtg gtggaccgag ctctacgccg
     2161 aatacgagca tacgcccggc agcggcggcg gctggtcggc attcgaatgg gagaaccgct
     2221 ttcagttcac agagccgggc caatcctggg tcgacgtcgg cgcgctggtc gagctcgacc
     2281 gagtgcatcc aggcaccggc aaggaagtcc gcgccggtat cctgctggaa aaggagatcg
     2341 acgagacgac gttgacattc aactggctgc ttaaccgggc tttcgccgac gaagagtcct
     2401 cgcactggga gcaggtgtac cgcgtgcaat gggcgtggcg tcttcgtccg gagatccagc
     2461 cgctggtgca atggcagagc gacgagcaca gcgcttacgc gggccccggc atcaaaggga
     2521 agttgagaat cgcagggcaa cacgtcgaat atcacctgac gtggctgcac aggatcagcg
     2581 gcgacatacc gaaagacgca gtgcgcgtgc aattggagta cgagttctag tgtagtctga
     2641 cgcttcgagc gcgccggccg cacgcgctgg cgcccgacgg cggccgcagc gaggcggtcc
     2701 ggtcgcgccg acggcaggaa cttcgcgccg caacgcgatc caatccaatt tgcctcgtt
//
LOCUS       DATANS010000080         1694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_508725, whole genome shotgun sequence.
ACCESSION   DATANS010000080 DATANS010000000
VERSION     DATANS010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508725"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1624)
                     /gene="gshA"
                     /locus_tag="VHT22_01205"
     CDS             complement(41..1624)
                     /gene="gshA"
                     /locus_tag="VHT22_01205"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX3136195.1"
                     /translation="MSSSGIDRAFERRLASLINAREPRVLQGGLKGVEKESLRVSPDG
                     HIEQSDHPRELGSALTHEHVTTDYSEALIELVTPAFRESWELLQYLCDLHQFVYRHLG
                     DELLWATSMPCIIDGDPSIPIARYGRSNIGRMKEVYRNGLSWRYGRMMQAISGVHYNY
                     SFPMHFWPVLAATLQAPRDDQDFISSQYFGLLRNYRRYGWLVLYLFGNSPAVCGSFVR
                     GREHSLQTFAPGTLYEPHATSLRMSDLGYRNKNQSGVQISVNSLEEYVRDLSAAVSTP
                     NPEYERIGVKVDGEYRQLNTNLLQIENEYYSYIRPKRVARSGERPTNALQRGGVEYVE
                     FRALDVSAFDPVGVNQNKLRFLEAFAALCVVKPSEPIDAAEWQQLDANHVLVAHRGRE
                     PGLQLNRNGSPIELRAWALELIDSMRGICEMLDHGDAQRPYAAALELQQAKIDDPRLT
                     PSARTLEELRSTGESFADMALRMSRVHKAYFLDLYPPNEQRLAEFAAEARESLERQAA
                     VEAADRVTFDQFLANYFRT"
BASE COUNT          310 a          535 c          539 g          310 t
ORIGIN      
        1 cactctagtc ctcgtcgtga cggtgcgcca gtgccgaact ctacgttctg aagtaattcg
       61 ctagaaactg gtcgaaagtc acgcggtcgg cggcttccac ggcagcctgg cgttccagcg
      121 actcgcgcgc ctcggcggcg aattccgcca ggcgctgctc gttcggcggg tacaggtcca
      181 ggaagtacgc cttgtggacc cgggacatgc gcagcgccat gtccgcgaac gactcgccgg
      241 tcgaacgcag ctcctcgagc gtgcgtgccg acggcgtcaa gcgcggatcg tcgatcttgg
      301 cttgctgcaa ctcgagcgcc gccgcgtacg gccgctgcgc gtcgccgtga tcgagcatct
      361 cgcagatgcc gcgcattgaa tcgataagct cgagagccca tgcgcgcaac tctatcgggc
      421 ttccgttgcg gttcagttgc agacccggct cgcggccacg atgcgcaacc aggacatggt
      481 tcgcgtcgag ttgctgccac tctgccgcgt cgatcggctc gctcggcttc acgacgcaaa
      541 gcgcagcaaa cgcctccagg aagcgcagct tgttctgatt cacaccgacc ggatcgaacg
      601 cgctgacgtc gagagcgcgg aactcgacgt actccacgcc gccgcgttgc agagcattgg
      661 tcggccgctc gccggagcga gcgacgcgtt tcgggcggat gtagctgtaa tactcgttct
      721 cgatctgcaa caggttggtg ttgagctggc gatactcgcc atcgaccttc acaccgatgc
      781 gctcgtactc gggattcggc gtgctcaccg ctgccgacaa atcgcgcacg tattcttcga
      841 ggctgttaac cgagatctgc acgccagact ggttcttgtt gcgatagccg agatcgctca
      901 tgcgcagcga agtcgcgtgc ggctcataca gcgtgccggg cgcgaacgtc tgtagcgagt
      961 gttcgcgtcc gcgcacgaac gagccgcaca ccgccggcga attgccgaac agatacagca
     1021 ccagccaacc gtagcggcga tagttgcgca acagtccgaa gtactgcgag gagatgaagt
     1081 cctgatcatc gcgcggtgct tgcaacgtcg cggccagcac cggccagaag tgcatcggga
     1141 acgaatagtt gtaatgcacg ccggagatcg cctgcatcat ccggccgtag cgccaggaga
     1201 ggccattgcg atagacctct ttcatgcggc cgatgttcga gcgcccgtag cgcgcgattg
     1261 gaatgctcgg atcgccgtcg atgatgcacg gcatgctcgt cgcccacagc agctcgtcgc
     1321 cgagatggcg atagacgaac tgatgcaagt cgcacaggta ctgcagcagc tcccacgatt
     1381 cgcggaacgc cggcgtgacc agctcgatca gcgcttcgga gtaatcggtc gtgacgtgct
     1441 cgtgcgtgag cgcggagccg agctcgcgcg ggtgatcgct ctgctcgatg tggccatcgg
     1501 gcgagacgcg cagcgattcc ttctcgacgc ctttcaagcc gccttgcagg acgcgcggct
     1561 cgcgcgcgtt gatcagagag gcgagccgtc gctcgaacgc gcgatcgatg ccggagctgc
     1621 tcatgttgat gctttggggc tcagaaccgg atgcggccga ccaggatgtc gcggaacatc
     1681 acgaagtcgc cggc
//
LOCUS       DATANS010000081         1820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_759794, whole genome shotgun sequence.
ACCESSION   DATANS010000081 DATANS010000000
VERSION     DATANS010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759794"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VHT22_01210"
     CDS             complement(<1..348)
                     /locus_tag="VHT22_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3136196.1"
                     /translation="MTRDANDGGGSGQPASPVPLLALRNVSKTFPGVRALDDVSLELH
                     AGEVHMLLGENGAGKSTLIKVLYGAYRADAGTILYEGKRVDIARPADARRLGIAVIFQ
                     EFSLVPFLDLAQNI"
     gene            complement(389..841)
                     /locus_tag="VHT22_01215"
     CDS             complement(389..841)
                     /locus_tag="VHT22_01215"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX3136197.1"
                     /translation="MAGNESTGIAQRIAAVHPRPDQERYMPYAPAVRVAGPADIVFIS
                     GSTSSPLYHRHPHVADEHRHPIDIESQTRRAMEGIKLVLDDQALTWRHVVKVTKYLTD
                     MRDMDGMVEVLSEYFGDWKPASTTICINSLSTPGARVELDMIAVRPLG"
     gene            complement(901..>1820)
                     /locus_tag="VHT22_01220"
     CDS             complement(901..>1820)
                     /locus_tag="VHT22_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX3136198.1"
                     /translation="SAGPASAAGERLAAFYKNQVDPHFALVRAGVEAAAKDMGAGPVT
                     HYAPTRPNNLAEQLSEIEDVTVKKPDAILFMAVDPRGVVPAIEKVNAAGIPLVNYNDR
                     VRGGKFVSYVAADDYNLGLDAGRYLIKALGGKGNVVIIEGVKGATTSDDRVRGFNKAI
                     EENPGVKLLASQPANYQRLQALQVMENLVQSNPSIDGVMAAADVMAFGAIEALEAAGK
                     KATKVVGIGGVPESIEAIKAGRLLATAEFNGYKMGCLATMAAVRSLRKETVPDTIVIK
                     GIVIDKTTTGPFEIPAEKRTCPKWEDYAK"
BASE COUNT          299 a          595 c          574 g          352 t
ORIGIN      
        1 gatgttttgc gcgagatcga ggaaaggaac aagcgagaat tcctgaaaga tcacggcgat
       61 gccaagccgc cgcgcatcgg ccggccgcgc gatgtcgacc cgttttccct cgtagaggat
      121 ggttccggcg tcggcacggt acgcgccgta gagcaccttg atcagcgtcg acttgccggc
      181 gccgttttcg ccgagcagca tgtgcacctc gcccgcatga agctcgaggc tcacgtcgtc
      241 gagtgcgcgc acgccgggaa aggtcttgct gacgttgcgc agcgccagaa gcggaaccgg
      301 cgaagcgggc tgcccggatc cgccgccgtc gttagcgtcg cgggtcacgg gcggcgatca
      361 cgggcgtccg atcacgcaac tgaggcgatc agccgagcgg acgcacggcg atcatgtcga
      421 gctctacccg cgcaccgggc gtggagagcg agttgataca gatcgtcgtg cttgccggct
      481 tccagtcgcc gaaatactcg ctcagcacct cgaccatgcc gtccatgtcc cgcatgtcgg
      541 tcaggtactt ggtgaccttc acgacatgcc gccaggtcag tgcctggtcg tcgaggacca
      601 gcttgatacc ttccatcgcg cgccgggttt gcgattcgat gtcgatcggg tggcgatgct
      661 cgtcggcgac gtgcggatgc cggtggtaga gcggcgacga ggtcgagccc gagatgaaaa
      721 cgatgtcggc cggcccggca acacgaacgg ccggcgcata cggcatgtag cgctcctgat
      781 ccggtcgcgg gtgcaccgcc gcgatgcgct gtgcgatacc ggtgctctca ttgcccgcca
      841 tctgccctcc ttgcgaaacc agggcgcgcg acccttggcg atacgcgcgc ttcgcaatca
      901 ctatttcgcg taatcctccc atttcgggca ggtgcgtttt tccgccggaa tctcgaacgg
      961 tcccgtcgtg gtcttgtcga taactatccc cttgatcacg atggtgtcgg gcacggtttc
     1021 cttgcgcagg ctccgcaccg ccgccatcgt ggccaggcag cccatcttgt agccgttgaa
     1081 ctcggcggtc gcgagcaatc gtcccgcctt gatcgcttcg atcgattccg gcacgccgcc
     1141 gataccgacg accttggtcg ccttcttgcc cgcggcctcc agcgcctcga tcgcgccgaa
     1201 tgccattacg tcggcggcgg ccatcacgcc gtcgatcgac ggattcgact ggaccaggtt
     1261 ttccatcacc tgcagcgctt ggagccgctg ataattagcc ggctgggaag cgagcaattt
     1321 gacgcccggg ttttcttcga tcgccttgtt gaagccgcgc acacgatcgt cgctggtggt
     1381 cgcccccttg acgccttcga tgataacgac gttacccttg ccgccgagcg ccttgatcag
     1441 atagcggcct gcatcgagac ccaggttgta atcgtctgcg gcgacgtagc ttacgaactt
     1501 gccgccgcga acgcgatcgt tgtaattgac cagcggaatg cccgcggcgt tgaccttttc
     1561 gatcgccggc acgacgccgc gcggatcgac cgccatgaac aggatcgcgt ccggcttctt
     1621 gacggtgacg tcctcgatct cggaaagctg ttcggcaaga ttgttcggcc gggtcggcgc
     1681 gtaatgcgtc accgggcctg cgcccatgtc cttcgccgca gcctcgacac cggcgcggac
     1741 cagcgcgaag tgcgggtcga cctggttctt gtagaaggcg gcaaggcgct caccggcggc
     1801 gctcgccggt ccggcagaca
//
LOCUS       DATANS010000082         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_46673, whole genome shotgun sequence.
ACCESSION   DATANS010000082 DATANS010000000
VERSION     DATANS010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46673"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /gene="pcnB"
                     /locus_tag="VHT22_01225"
     CDS             <1..1010
                     /gene="pcnB"
                     /locus_tag="VHT22_01225"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEX3136199.1"
                     /translation="DEHGRLLSDNVFGTQAQDAARRDFTINALYFDPTTEEVWDYVGG
                     VADIKARRLRLIGDPVTRYREDPVRMLRGARLAAKLGISIEKKTAAPIPVLAPLIHNV
                     PPARLFDEMEKILLSGHAAESVKSLRRHGLHHGVLPLLDVILEQPLGQRFVDLALANT
                     DRRVGEGKSVTPAFLFASLLWNEVLASWNAAQQRGERPTPALFEAMDRVLEQQAERLA
                     IPRRFEATIKEIWALQPRFEQRAGQRPFRLLEHPRFRAAYDFLVLRCESGELQGELAG
                     LADWWTKFQDADTAERERMLKPDEAPKKRRRSRSRGRKREPAANEAAADAAAEGEESA
                     E"
     gene            1015..1551
                     /gene="folK"
                     /locus_tag="VHT22_01230"
     CDS             1015..1551
                     /gene="folK"
                     /locus_tag="VHT22_01230"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX3136200.1"
                     /translation="MQHRFRHLPGASLAYVGIGSNLAHPRRHVARAVAEMARLPRTRV
                     VAVSPNYLSAPLGATAPQPDYVNAVAALVTSLAAPALLKRMRTIEYRHGRPPAKQRVR
                     NAARTLDLDLLLFGRRRSRQRQLTLPHPRMHQRAFVLRPLLDIAPAAVIPGRGAARRW
                     LRGLHGQRIARTRRHTRL"
     gene            1564..2208
                     /locus_tag="VHT22_01235"
     CDS             1564..2208
                     /locus_tag="VHT22_01235"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEX3136201.1"
                     /translation="MDIEKCRYIVVEGPIGAGKTSLARTLALSLGADTLLEAPDENPF
                     LARFYEDMRRFALPTQLNFLFQRVDQLRGLAQLDMFARHTVADFLLDKDPLFARLNLS
                     DDEFALYDKVYSHLKLQTPTPDLVIYLQAPVTTLIERVHRRGVGYERGISEHYLARLA
                     DAYSRYFYQYAGAPLLIVNSERLNFVDEPGHLQLLRSRIGTMRGQREFFNLGAS"
BASE COUNT          385 a          794 c          729 g          383 t
ORIGIN      
        1 ccgacgaaca tgggcggctg ctgtcggaca acgtcttcgg cacgcaggca caggacgcgg
       61 cccggcgcga cttcactatc aacgccctgt atttcgatcc cacgaccgag gaagtctggg
      121 actacgtcgg cggtgtggcc gacatcaagg cgaggcggct gcggctgatc ggcgacccgg
      181 ttacacgcta ccgcgaagac ccggtccgca tgctgcgcgg ggcgcgcttg gccgcgaagc
      241 tcggcatcag catcgaaaag aagaccgcag cgccgattcc ggtgctggca cctctgatcc
      301 acaacgtccc tccggcgcgg ctgttcgacg agatggaaaa gatcctgctg tccggtcacg
      361 ccgccgaatc ggtcaaaagt ctgcgccggc acgggctcca tcacggcgtg ctgccgctgc
      421 tcgacgtgat cctagagcag ccgctgggcc agcgctttgt cgatctcgcg ctggccaaca
      481 ccgaccggcg cgtcggcgag ggcaagtcgg tgactccggc cttcctgttc gcctcgttgc
      541 tatggaacga ggttctcgcc agctggaacg ccgcgcagca gcgcggcgag cgcccgacgc
      601 cggcgctgtt cgaggcgatg gatcgcgtgc tcgagcagca ggccgagcgc cttgccattc
      661 cgcgccgctt cgaggccacg atcaaggaaa tctgggcgct gcagccgcgc ttcgaacagc
      721 gtgccgggca gcgcccgttc cgcctgctcg agcacccgcg ctttcgcgcc gcgtacgact
      781 ttctggttct gcgctgcgag agcggagaat tgcaaggcga gctcgcaggt ctcgccgact
      841 ggtggacgaa gttccaggac gccgataccg cggagcgcga gcggatgctg aagcccgacg
      901 aggcgccgaa aaaacgtcgc cgttcgcgca gccggggacg caagcgcgag cctgcggcaa
      961 acgaagccgc agcggacgcc gcagccgagg gcgaagaaag cgccgagtag gccagtgcag
     1021 catcgattcc ggcatctgcc cggggcgtcg ctcgcctatg tcggtatcgg cagcaacctc
     1081 gcccatccgc gccgccacgt cgcgcgcgcg gtcgctgaaa tggcgcgcct gccgcgcacc
     1141 cgcgtggtcg cggtttcgcc caactatctg agtgcgccgc tcggggccac cgctccacag
     1201 cccgactacg tcaacgcggt tgcggcactg gtaacgtcgc tcgctgcgcc tgccctgctg
     1261 aagcggatgc gaacgatcga gtatcgccac ggcaggccgc ctgcgaagca acgcgtgcgc
     1321 aacgccgcgc ggacgctcga cctcgatttg ctactattcg gccgtcgccg ttcgcgacaa
     1381 cgacagctga ccttgccgca tccgcgcatg catcagcgcg cgttcgtgct gaggccgttg
     1441 ctggacatcg cgcccgcggc tgtcattccc ggccgtggcg ctgcccgccg ttggttgcgc
     1501 ggactgcacg gacagcgcat cgcccggacg cgccgccaca cccggttgta gcgacaagct
     1561 gccatggaca tcgagaaatg ccgctacatc gtcgtcgagg gcccgatcgg cgcgggcaag
     1621 accagcctcg cccgaacgct tgcgctttcg ctcggcgccg atactttgct cgaggcgccg
     1681 gatgagaatc ccttcctcgc ccggttctac gaggacatgc gccgcttcgc actgccgaca
     1741 cagctcaatt tccttttcca gcgcgtcgac cagctgcgcg gattggcaca gctcgacatg
     1801 ttcgcccgac atacggtcgc cgattttctg ctcgacaagg atcctctgtt cgcgcggctc
     1861 aatctttccg acgacgaatt cgcgctctac gacaaggtat attcgcatct gaagctgcaa
     1921 acgccgacac ccgatctggt gatctatctg caggcgccgg tcacgacgct gatcgagcgc
     1981 gtgcacaggc gcggtgtcgg ttacgagcgg ggaatatccg agcactatct ggcgcggctg
     2041 gccgacgcgt acagccgcta tttctaccag tatgccggcg cgccgctgtt gatcgtcaac
     2101 agcgagcgcc tgaacttcgt cgacgagccc ggccatttgc agctgctgcg cagccgtatc
     2161 ggcacgatgc gcggacaacg cgaattcttc aatctcggcg caagctagct tgccgctagc
     2221 caatcgcgca gccgccgctc actcggtttt gggccgcacg cgcggccgtc gcagcgccag
     2281 cgtgttgccc a
//
LOCUS       DATANS010000083         3636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_129949, whole genome shotgun sequence.
ACCESSION   DATANS010000083 DATANS010000000
VERSION     DATANS010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129949"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..1217
                     /locus_tag="VHT22_01240"
     CDS             249..1217
                     /locus_tag="VHT22_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004535190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3136202.1"
                     /translation="MQYRRLGSSGLKLSELSFGSWVTYGNQLAEDSARECMAAAHDAG
                     VNFFDNAEVYARGHSETIMGAALAKLGWRRSSYVVSTKFYWGLNDGPNEKNTLNRKYL
                     MQAIDGSLKRLKLDHVDLVFCHRPDPDTPLEEVVWAMHDMIRTGKALYWGTSEWEAAA
                     IAAAWHVADKHHLAKPVMEQPQYNLFHRERVEREYARLYDDPGIGLTTWSPLASGLLS
                     GKYNDGIPPGSRATIKGYEWLAERIVDPAKLAQVRSLAPVAADLGCTLAQLALAWCLK
                     NPHVTTVITGASRASQVVENMRCLEVVPKLDAEIMARIDGIVGAPV"
     gene            1271..2308
                     /locus_tag="VHT22_01245"
     CDS             1271..2308
                     /locus_tag="VHT22_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX3136203.1"
                     /translation="MSNPLESWTEEQRSAFLYRACAAAEAGTPRADLFMRLGGEAEAQ
                     AAIWRAQLTASGKPAPPPFVPDIRARLVVRLIKLFGPAPLRGILAAMKVRGMAIYTKG
                     EPGGHVTPVAGSIERRHRGLGGGGYLRAAVFGINDGLVSNASLIFGVAGANSDPGLVV
                     LTGVAGLTAGAFAMAAGEFVSVRSQRELFEYQIGLEREELAEYPDAEAQELALIYEAK
                     GLAPGEARRLAKRLVADPEHALDTLAREELGLNPDELGSPWQAAVSSFLSFAVGAGIP
                     LLPFVFAARANALTITGLLTALALFTVGATLSLFTGRGALPSGIRMLLLGGLAAAVTF
                     AVGHLFGVSAG"
     gene            2323..3507
                     /locus_tag="VHT22_01250"
     CDS             2323..3507
                     /locus_tag="VHT22_01250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEX3136204.1"
                     /translation="MHRVTLQVGRDKSLRLKHPWVFSGAIAMVDGSPASGETVAIHAA
                     DGQFMATAAWSPSSSIRARVWSFDARETIDDAFIAGRVRAAVAARKPMLDAAHTGCRL
                     VHAESDGLPGVIADRYGEVVVVQLHNAGAEAWRDSIVAALVDATGCAAVYERSDAEVR
                     ALEGLPPRTGTAYGALPERVSLLEDDLAYDIDVATGQKTGFFLDQRENRRRIRALATR
                     RDVLDCFCYTGGFTVAALAGDAQSVLAIDSSADALAQAKRNAAQNGIDDTRCAWRDAD
                     VFAELRKLRDSAKTFDLIVLDPPKFAPTARHMEKAARAYKDINLLALKLLRPGGLLAT
                     FSCSGGISAELFQKIVAGAALDAAADAAIVGRFAPGADHPIALNFPEADYLKGLLVRK
                     KA"
BASE COUNT          581 a         1188 c         1230 g          637 t
ORIGIN      
        1 gcgtggcggc catcgggaga caaggtcggc gaaccgacgc gcttgatcga ccatagatcg
       61 gcgacggaga gcggacgttt tttcatgggc atgggatttc catggcaacg atgatgggga
      121 gacagggcgc tcgtggcgcg ccggcggatt gtacgcttgc cggggacggc gggcaccggc
      181 cgcccttcag gtagaatatc cgctccggcg tcgcgttcgc gacgcaacgc ttctgtccag
      241 aggttcaaat gcagtaccgc cggctcggca gctccggtct taagctcagc gagctttcgt
      301 tcgggtcgtg ggtgacctat ggcaaccagc tcgccgagga cagcgcgcgc gagtgcatgg
      361 ccgccgcgca cgacgccggc gtcaattttt tcgataacgc cgaggtctat gcccgcggcc
      421 actccgaaac catcatgggc gcggcgctcg ccaagctcgg ctggcggcgc tcgtcgtatg
      481 tcgtgtcgac caagttctac tggggcttga acgacggccc gaacgaaaag aacacgctga
      541 accgcaagta cctgatgcag gcgatcgacg gctcgctcaa gcggctgaag ctcgatcacg
      601 tcgatctggt tttctgccac cggcccgatc ccgacacgcc gctggaggaa gtcgtctggg
      661 cgatgcacga catgatccgc accggcaagg cgctgtactg gggcacgtcc gagtgggaag
      721 cggcggcgat cgcggcggcc tggcacgtcg ctgacaagca tcatctggcc aagccggtga
      781 tggagcagcc gcaatacaat ctgttccatc gcgagcgggt cgagcgagaa tacgcgcgcc
      841 tctatgacga tccgggtatc ggactcacga cctggagtcc gctggcgtcg ggacttctgt
      901 cgggcaagta caacgacggc attccacccg ggtcgcgcgc aacgatcaaa ggctacgaat
      961 ggcttgccga acgcatcgtc gacccggcca agctcgcgca ggtgcgctcc ctcgccccgg
     1021 ttgccgccga tctcggctgt acgctggcgc agctcgcgct cgcctggtgc cttaagaacc
     1081 cgcatgtcac gacggtgatc accggcgcca gccgcgcgtc gcaggtcgtc gagaacatgc
     1141 gctgccttga ggtcgtaccc aagctggatg ccgaaatcat ggccaggatc gacggcatcg
     1201 tcggcgcgcc ggtttgatgg tttggttcga tcgtgtgtgc gcgtaatggt ctccctttga
     1261 gtcgttcacg atgagcaatc cgctggaaag ctggaccgag gagcagcgct ccgcatttct
     1321 ctatcgcgcc tgcgcagccg cggaagccgg cacgcctcgc gccgatctgt tcatgcgcct
     1381 gggcggcgag gcggaggcgc aggcggcgat ctggcgcgcc cagctcaccg ccagcggcaa
     1441 gccggcaccg ccgccgttcg ttcccgatat tcgtgcacgc ctggtcgtgc gcttgatcaa
     1501 gctgttcggc cccgctccgc tgcgcggcat tcttgccgcg atgaaagtcc gcggcatggc
     1561 gatttacacc aagggagaac cgggcgggca tgtcacgcct gtggcgggca gcatcgagcg
     1621 ccggcatcgt ggcttgggcg gcggcggcta tctgcgcgcg gcagtgttcg gaatcaacga
     1681 tggcctggta tcgaacgcga gcctcatttt cggcgtcgcc ggtgccaact ctgatcccgg
     1741 attggtcgtg ctgacaggcg tcgcgggtct gaccgcgggt gcattcgcga tggccgcggg
     1801 cgaattcgtt tcggtgcgct cgcaaagaga actgttcgaa taccagatcg ggctggagcg
     1861 cgaggagttg gccgagtatc cggacgccga ggcgcaggag cttgcgctga tctacgaggc
     1921 gaaaggcctg gcccccggcg aggcgcgccg actcgccaag cggctggtgg ccgaccccga
     1981 gcacgcgctc gatacgctgg cgcgcgagga gctgggcctc aaccccgacg agctcggctc
     2041 gccctggcaa gcggcggtgt cctcttttct gtcgtttgcc gtcggcgccg ggattccttt
     2101 gctgccattc gtcttcgccg cccgcgcgaa cgcgctgacg atcaccggat tgctgactgc
     2161 gctggcgctg ttcaccgtcg gcgcgacgct gtcgctgttc accggccgcg gcgcgctgcc
     2221 gtccggcatt cgcatgctgc tgctgggcgg actcgcggca gcggtgacgt tcgcggtcgg
     2281 ccatctgttc ggcgtcagcg ccggctgatt cgaaaagcgc ccgtgcatcg cgttacgctg
     2341 caggtcgggc gagacaagtc gctgcggttg aagcatccgt gggtgttttc tggtgcgatc
     2401 gccatggtcg acggctcacc cgcgagcggc gagaccgttg cgatccacgc cgccgacggc
     2461 cagttcatgg cgacggccgc ctggagccct tcctcgtcga ttcgcgcccg cgtatggagc
     2521 ttcgatgcgc gcgagacgat cgacgatgca ttcatcgccg gccgggtgcg cgccgccgtg
     2581 gcggctcgca agccgatgct cgacgccgca cacaccggtt gccgcctggt gcacgccgaa
     2641 tccgacgggc tgcccggcgt gatcgccgac cgctacggcg aggtggtcgt cgttcagctg
     2701 cacaatgccg gcgcggaagc atggcgcgac agcatcgtcg cagcgctggt cgacgctacc
     2761 ggctgcgcgg cagtctacga gcgctccgat gcagaagttc gagccctcga aggcctgcca
     2821 ccgcgcaccg gcaccgctta cggcgcactg ccggaacgcg tgagcctgct cgaggacgat
     2881 ctcgcctatg acatcgatgt ggccaccggc cagaagaccg ggttcttcct cgaccagcgc
     2941 gagaatcgcc gtcgcatccg cgctcttgcg acgagacgcg acgtgctgga ctgtttttgc
     3001 tataccggcg gctttaccgt cgcggcgctg gctggcgacg cacagtcggt gctggctatc
     3061 gacagttcgg ccgatgcgct ggcgcaggca aagagaaacg ccgcgcagaa cgggatcgac
     3121 gacacgcgct gcgcgtggcg cgacgccgat gtgttcgccg aattgcgcaa gctgcgcgac
     3181 agcgcaaaga ccttcgacct gatcgtgctc gacccgccga agttcgctcc gacggcccgg
     3241 cacatggaaa aagcggcgcg cgcgtacaag gacatcaacc tgctcgcgtt gaagctgttg
     3301 cgccccggcg gtctgctcgc aacgttctcc tgctccggag gcatctcggc cgagctgttc
     3361 cagaagatcg tcgccggcgc cgcgctggat gccgcggcgg acgcggcgat cgtcggacgc
     3421 tttgcgccgg gcgccgacca cccgatcgcg ctcaactttc ccgaagccga ctatctgaag
     3481 ggcctgctcg tgcgcaagaa ggcttaaggc gagcgtcgtc cgctgccggt agccggcgag
     3541 caggcaggac ggcgacgctg ccgccctgct cccgccggtc tatctcagga tttgcgcgta
     3601 gtcaggcagg atttcatgaa cgtcttgcgt tccgcg
//
LOCUS       DATANS010000084         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_424872, whole genome shotgun sequence.
ACCESSION   DATANS010000084 DATANS010000000
VERSION     DATANS010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424872"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VHT22_01255"
     CDS             <1..366
                     /locus_tag="VHT22_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX3136205.1"
                     /translation="LTGACVIALGHVASGLFANDDTLAEQLLAAGQTSRDRAWHLPLW
                     DDYQEQLKSNFADFANIGGRPAGSVTAACFLARFTKKFKWAHLDIAGVAWKSGAAKGA
                     TGRPVPLLTQYLIDRATKP"
     gene            509..937
                     /locus_tag="VHT22_01260"
     CDS             509..937
                     /locus_tag="VHT22_01260"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEX3136206.1"
                     /translation="MTQIDFYFHVENKLHTACALSAKACARGLRVLAFCADADAGMKF
                     SRLLWTAQAVSFIPHCTPGDALAAVTPVIVDHDGANPPHDEVLINLRPEWPPFFSRFQ
                     RLIEIVSLDEEDRSQARARFKFYRDRGYEIKSHDLSKSPQ"
     gene            952..>1712
                     /locus_tag="VHT22_01265"
     CDS             952..>1712
                     /locus_tag="VHT22_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX3136207.1"
                     /translation="MSEPRDRDDISPSDDVLGRADALLNRHRVAAKPAMDAGAIPTLT
                     QPLARDLDESAIPTLTDIVTTPVRAPAAASAAHADNPAAEQPAQSGEIITRVQAQNLE
                     HGVYQKLKQELDAQIADVLHSRFMPDVAGALDQALNKISSELKTNIDAMVRASIEQTL
                     QKQLEPLRLTDAAPPAEPVEPPAPAIMPDFSAPPPPSPPPRMELAKSFEPHAIENYWY
                     PRWESRGYFAHSNDTSRQPYCIQLPPPNVTGTLHMG"
BASE COUNT          340 a          582 c          513 g          277 t
ORIGIN      
        1 ctgaccggcg cctgcgtgat cgcgctcggc cacgtggcga gcggcttgtt cgccaatgac
       61 gacacactcg cggagcaatt gctggcggcg ggccagacct cgcgcgaccg cgcctggcac
      121 cttccgctct gggacgatta ccaggaacag ctcaagagca actttgccga tttcgccaat
      181 atcggcggtc gcccagccgg cagcgtgacc gcggcgtgct ttctcgcgcg ctttacgaaa
      241 aaattcaagt gggcgcatct cgacatcgcc ggggtcgcct ggaagtcggg cgcggccaag
      301 ggcgcgacgg gacggccggt gccgctgctc acgcagtatc tgatcgaccg cgcgaccaaa
      361 ccttgacccg ccggaaccgc aaagctaaaa caaccacatt tgccacgttc cggcgccggt
      421 cgcgcgaatg cgtccaggct tgcgatcgcg ccaagattga caccgtccgt ctgcgcggtg
      481 ccggcggcaa agctctaagg ggtggggttt gactcagatc gatttttact ttcacgtcga
      541 aaacaagctg cacacggcgt gcgcgttaag cgccaaagcc tgcgcgcgcg gcctgcgcgt
      601 gctcgcgttc tgcgcggatg ccgatgccgg catgaagttc agccggctgc tgtggaccgc
      661 ccaggcggtc agtttcattc cgcattgcac gcccggcgat gcgctcgcgg cagtcacccc
      721 cgtgatagtc gatcatgacg gcgccaatcc gccgcacgac gaagtactga tcaatctgcg
      781 cccggaatgg ccgccctttt tcagccggtt ccagcgccta atcgagatag tgagcctgga
      841 cgaggaagac cgcagccagg cgcgcgcgcg cttcaagttt taccgggacc gtggctacga
      901 aatcaaaagc cacgatcttt cgaaaagccc tcaatagatg cgcgcccagc catgagcgag
      961 ccgcgcgacc gcgacgatat ttcgcccagc gatgatgtat tgggccgcgc cgatgcgctc
     1021 ctgaaccggc accgtgtcgc cgccaagccc gccatggatg cgggcgcgat accaacgctg
     1081 acgcagcccc tcgcccgcga tctcgacgag tcggcgatac ctacgctaac cgacatcgtg
     1141 acaacgccag tgcgcgcacc cgccgctgcc tcggcagcgc acgctgacaa tccggccgcc
     1201 gaacaaccgg cgcaaagcgg cgaaatcatc acgcgcgtgc aggcgcaaaa tctggaacac
     1261 ggcgtgtatc agaaattgaa gcaggagctc gacgcgcaga tcgccgatgt gctgcacagc
     1321 cgtttcatgc ccgatgttgc cggcgcgctt gatcaggcgt tgaacaagat aagcagcgag
     1381 ctcaaaacga acatcgatgc gatggtgcgc gcctcgatcg agcaaactct gcaaaaacaa
     1441 ctggagccgt tgcgcctcac cgacgctgcg ccgccggcgg aaccggtcga gccgccagcg
     1501 cccgctatca tgccggactt ttctgccccg cccccgccct cgcccccgcc ccggatggaa
     1561 ctcgccaaga gctttgagcc gcacgcgatc gaaaactact ggtatccgcg gtgggaaagc
     1621 cgcggttatt tcgcgcactc gaacgacacg tcgcggcagc cttattgcat ccagctaccg
     1681 ccgccgaacg tgaccggcac gctgcacatg gg
//
LOCUS       DATANS010000085         2036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_172750, whole genome shotgun sequence.
ACCESSION   DATANS010000085 DATANS010000000
VERSION     DATANS010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172750"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VHT22_01270"
     CDS             <1..459
                     /locus_tag="VHT22_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEX3136208.1"
                     /translation="ILACGNGGSASDAQHFAAEMVGRFERERPELPALSLATDTSILT
                     AVANDYDFSQVFAKQVRALGRSGDVLLALSTSGNSANVALAIDAAHEREMRVVALTGR
                     GGGRIGELLTGVDVHLCVPHSVTARIQEVHLLTIHCLCDAIDTNLLGEES"
     gene            492..1082
                     /locus_tag="VHT22_01275"
     CDS             492..1082
                     /locus_tag="VHT22_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX3136209.1"
                     /translation="MKRFDTIMRIAATLSLAAGATLLNGCIPLVIGGAVVTAQVATDR
                     RTTGAQLDDEFIEDKAIAQISDRLKSDIHVNVTSYNGIALLTGEAPTEASKNEVAQVV
                     RSMPKVRIVQNELVVGPTSSFSARTNDTVTTSKVKSRFVEAYNKFQINHVKVVTERNV
                     VYLMGIVKRDEGDAAAEIARTTSGVERVVKVFEYIS"
     gene            1133..1585
                     /locus_tag="VHT22_01280"
     CDS             1133..1585
                     /locus_tag="VHT22_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX3136210.1"
                     /translation="MATAESRAAQIAALPRPLVFTNGVFDILHRGHVSYLDHARRLGA
                     SLVVGVNSDASARRLGKGGDRPINSLDDRIAVLAALESVSLAIAFDEDTPLALVLECH
                     PDVIAKGADYTAATTVGAAEVINWGGRFEAIPLLPGRSTSALLTRLRR"
     gene            1649..1972
                     /locus_tag="VHT22_01285"
     CDS             1649..1972
                     /locus_tag="VHT22_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX3136211.1"
                     /translation="MFQTIDALELSRLMQSGDAPRVVDVRTAAEVSRGTIGGALHIEL
                     STLPARLSELDSDAPVVVFCQSGARSAQACAYLAERGFRRAFNLGGGLAGWVRAGLPL
                     NGLDR"
BASE COUNT          363 a          665 c          669 g          339 t
ORIGIN      
        1 atcctggcct gtggcaacgg cggctcggcg tccgacgccc agcatttcgc ggccgagatg
       61 gttggacgct tcgagcgcga gcgcccggag ttgccggcgc tgtcgctggc gaccgatacc
      121 tcgatcctga ccgcagtggc caacgactat gacttcagcc aggtgttcgc caagcaggtg
      181 cgcgcgctgg gacgcagcgg cgacgtgctg ctcgcgctgt cgacgtcggg caactcggcc
      241 aatgtcgcgc tggcgatcga tgccgctcac gagcgcgaaa tgcgggtcgt tgcgctgacg
      301 ggccggggcg gaggccgcat cggtgaactc ctcaccggcg tcgatgtcca cctctgcgtc
      361 ccgcattcgg tgaccgcccg catccaggaa gtgcacctgt tgacgataca ctgcctgtgt
      421 gacgcgatcg ataccaacct gctcggcgaa gagagctgaa aaaactgaac cctggggaaa
      481 cgcgaggaat catgaagagg ttcgatacca tcatgcggat tgcggcaacc ttgtcgctcg
      541 ccgccggcgc gacgctgctc aacggctgca ttccgctggt gattgggggt gccgtggtga
      601 ccgcccaggt cgccaccgac cggagaacga ccggtgccca gctcgacgac gagttcatcg
      661 aggacaaggc aatcgcgcag atctccgatc gtctcaagag cgatatccac gtcaacgtga
      721 ccagctacaa cggcatagcg ctgctgaccg gcgaggcccc gaccgaggcc tcgaaaaacg
      781 aggtggcgca ggtcgtgcga tcgatgccga aagtgcgcat tgtgcagaac gagctcgtcg
      841 tcggcccaac cagctcgttt tccgcccgga ccaacgacac ggtgaccacc tccaaggtca
      901 agtcacgatt cgtcgaggca tacaacaagt tccagatcaa tcacgtcaag gtcgtcaccg
      961 agcgcaacgt cgtctacctg atgggcatcg tgaagcgcga cgagggcgat gccgccgccg
     1021 agatcgcccg cacgaccagc ggcgtggagc gcgtcgtcaa ggtgttcgaa tacatcagtt
     1081 gagcgcttcg tccgaccggc ccggggcaga ggggccgagc ttcgcgtcca agatcgctac
     1141 ggccgagagc cgcgcggcgc agatcgcggc gctgccgcgg ccgctggtgt tcaccaacgg
     1201 cgtgttcgac atcctgcacc gcggccatgt cagctacctc gaccacgcgc gccggctcgg
     1261 cgcctcgctg gtcgtcggcg tcaacagcga tgcatcggcg cgccgcctcg gcaagggcgg
     1321 tgatcggccg atcaattcgc tcgacgaccg catcgcggtc ctcgccgctc tcgaatcggt
     1381 gagtctggcc attgccttcg acgaagacac cccgctggcg ctcgtgctcg aatgccatcc
     1441 cgacgtcatc gccaagggcg ccgactacac ggcggccacg accgtcggcg ccgccgaagt
     1501 catcaattgg ggcggacgct tcgaggcgat accgctgctg cccggacgct cgaccagcgc
     1561 gctgctgacg cgcttgcggc gctgaaagcg cgagggcgag gcggcacagc acgtttgcta
     1621 taatttgctg ctttagcgag cgaagtcgat gttccagacg atcgatgcac tcgagctctc
     1681 gcgcctgatg caaagcggag acgcgccacg ggtggtcgac gtgcgcaccg cggccgaagt
     1741 gtcccgcggg acgataggcg gcgcgctgca catcgagctg tcgacgttgc cggcgcgact
     1801 ctccgagctc gacagcgacg cgcctgttgt cgtcttttgt cagtcgggtg cgcggtcggc
     1861 gcaagcctgc gcttacctcg ccgagcgcgg ctttcgccgc gcattcaatc tgggcggagg
     1921 cctcgccggc tgggtgcggg caggcctgcc actgaatggc ctggaccgat aatggatcag
     1981 acagtgcaat tcgaacgtga agtcgatgcg cgcggcttga attgtccgct gccgat
//
LOCUS       DATANS010000086         1526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_675304, whole genome shotgun sequence.
ACCESSION   DATANS010000086 DATANS010000000
VERSION     DATANS010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675304"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VHT22_01290"
     CDS             complement(<1..889)
                     /locus_tag="VHT22_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3136212.1"
                     /translation="MTAAVLRQTPLPDPFRAGIASGWKVIDASALSADLALQADVAIV
                     GTGAGGATAAEILSEAGLSVVMIEEGPLATSSDFHMLEREAYPQLYQESAARKTRDKA
                     INILQGRCVGGGTTVNWTSSFRTPPTTLTHWSRTFGIAGFSEADLAPWFERVERRLNI
                     TPWEVAPNENNDVLRRGAEALGIPVATIRRNVKGCWNIGYCGVGCPTNAKQSMLVTAI
                     PAALSNGATLLTRTRAWTFDHSGGNVTALNCVALDPRGTDPSPRRIVVRAGTFIAAGG
                     AIGTPALLLRSRAPDPAAIV"
     gene            complement(889..1245)
                     /locus_tag="VHT22_01295"
     CDS             complement(889..1245)
                     /locus_tag="VHT22_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136213.1"
                     /translation="MLAGALPANDPVLLQETVAGVEQAIAGLPPAVRKEVDQLFALLS
                     FAPTRALIAGVWSTWHEASPASIGAFLDRWRDSRLALLRSAYGALHQLVFAAWYGNSN
                     AWPAIGYAGPPSLEVG"
BASE COUNT          265 a          494 c          530 g          237 t
ORIGIN      
        1 cgacgatggc tgccgggtcc ggcgcgcggc ttcgcagcag cagcgccggc gtgccgatcg
       61 cgccgccggc agcgatgaag gtcccggcgc gcacgacgat gcggcgaggg ctcggatcgg
      121 ttccgcgcgg gtcgagggcg acgcagttga gggccgtgac gtttccgccc gaatgatcga
      181 atgtccaggc acgcgtgcgg gtcagcagcg tcgcgccgtt ggacaatgcc gcgggaatcg
      241 ccgtgactag catcgactgc ttggcgttgg tcggacagcc gacgccgcaa tagccgatgt
      301 tccagcagcc tttaacgttg cgtcggatcg ttgccaccgg gatgccgagc gcttccgccc
      361 ctcgacgcag aacatcgttg ttctcgttcg gggcgacttc ccacggcgtg atattcagac
      421 ggcgttcgac gcgctcgaac cacggcgcca gatcggcctc gctgaagcca gcgatcccga
      481 acgtgcgcga ccagtgcgtc aatgtcgtcg gaggcgtgcg aaagctcgat gtccagttga
      541 ccgtcgtgcc gccgccgacg cagcgtccct gcaatatgtt gatcgctttg tcgcgggtct
      601 tgcgcgcggc ggactcctga tagagctgcg gataggcctc gcgctcgagc atgtgaaagt
      661 ccgaggaggt cgcaagcggc ccttcctcga tcatgaccac ggaaaggccc gcctcgctca
      721 gaatttcggc ggccgtggct ccgcccgctc cggtgccgac gatggcgaca tctgcctgca
      781 gcgcgagatc ggcactcaat gccgatgcat cgatcacctt ccagccgctg gcgatgccgg
      841 cacggaacgg atcgggcaac ggggtctgac gcaggaccgc cgcagtcatc agccgacctc
      901 gagcgacggc gggccggcgt agccgatcgc cggccaggcg ttcgagttgc cgtaccaggc
      961 ggcaaaaacg agctgatgca gtgcgccgta ggccgatcgc agcagagcca gtcggctatc
     1021 gcgccaccga tcgagaaagg cgccgatcga tgccggcgac gcctcgtgcc acgtcgacca
     1081 tacgccggcg atcagagcac gggttggcgc gaacgacagc agcgcgaaga gctggtccac
     1141 ctccttgcgc accgccggcg gcaaaccggc gatcgcctgt tcgacgcccg cgacggtctc
     1201 ttgcagcaac accgggtcgt tggccggtag cgcgccggcc agcatgacag gggcgatcgc
     1261 cgcgacgatc gcgcgcgcat cggcatcgag cgcgccgtat ggcgtcgact gcggtgttgt
     1321 cgtcgatgta tacagccagc gggcgagcag caggcctgca ccgccggcga tgccggcctt
     1381 gagcaatgtg cggcgactga tgcggggcga cgcggcggaa tcgggcatac ggaggaaccg
     1441 aagtggaggg acaacgacgc aatctagcgg tcgagtctag acaagacaac ctaattctct
     1501 ttggtcaggt tcgaggggcg cggccg
//
LOCUS       DATANS010000087         3689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_214377, whole genome shotgun sequence.
ACCESSION   DATANS010000087 DATANS010000000
VERSION     DATANS010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214377"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1972)
                     /gene="infB"
                     /locus_tag="VHT22_01300"
     CDS             complement(<1..1972)
                     /gene="infB"
                     /locus_tag="VHT22_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020831757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX3136214.1"
                     /translation="MPQTTIDKFATELKMPAGILLEQLAAAGVAGKKQGDKLSEQDKT
                     RLLDYLRKQHGGLDQKKKITLTRKQTTEIKAADSTGRARTIQVEVRKKRVFVKRDGAD
                     AAAATEEAVPVAPQPVVTATELAAREEEARKGAALIAKQQEEIQQKQEQAKKRKTKKE
                     KEEEDALAKAAEAEAAAVAKEAAPVAQPAATAKPEAKAKTEGTLHKPVAKPGAKKDKP
                     SRKPSATAFQEEAARRRALKLRGDVTSGAATAGWKQPKGGKHRHEEGDEVEEGAQALA
                     PEPLIREVAVPETITVADLARKMSVKAAEIIKAMMKLGTMVTINQMLDQETAMIVVEE
                     MGHKAIAAKLDDPDSLLAESAQATTAESLPRPPVVTVMGHVDHGKTSLLDTIRRTRVA
                     SGEAGGITQHIGAYHVETAKGVITFLDTPGHEAFTAMRARGAKVTDIVILVVAADDGV
                     MPQTKEAIAHAKAGNVPIVVAINKIDKPEANPDRVKQELVAESVLPEEYGGDTQFVQV
                     SAKTGAGIDNLLDAILLQAEVLELKAPKDAPAKGIVIESRLDKGKGPVATVLVNSGTL
                     KRGDIVLAGAVFGRVRAMTDETGKTVASAGPAIPVEIQGLSDVPLAGEDVMVLGDERK
                     AREIALFRQGKFRDVKLAKQQAAKLENMFEQLA"
     gene            complement(2006..3496)
                     /gene="nusA"
                     /locus_tag="VHT22_01305"
     CDS             complement(2006..3496)
                     /gene="nusA"
                     /locus_tag="VHT22_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX3136215.1"
                     /translation="MNRELLLLVDALAREKNVPKDIVFTALESALASATKKRFTQDID
                     ARVSIDRETGDYDSFRRWTVVPDEEHEEPARQIAITDAAERDPPLALGDVIEEPLEPV
                     EFGRIGAQAAKQVILQKIRDAEREQILNDFLDRDDNLLTGTVKRIERGNVIVESGRIE
                     GMIPRDQLIPKENLRVGDRVRAYVIKIEAQARGPQLLLSRITPDLVVRLFELEVPEIE
                     EGLIEIKAAARDPGVRAKIAVKSNDPRLDPQGTCIGMRGSRVTAVTNELAGERVDIVL
                     FSEDPAKLVINALSPAEISSIVVDEERHSMDVVVDEENLAMAIGRNGQNVRLASELTG
                     WEINLMGVEESQQKHQVESERTRALFIDKLDVDQEVADILIQEGFTSLEEIAYVPLNE
                     MLEIDAFDEDTVNELRSRARNALLTEAIVREESVGNVAEDLLNLEGMDNETAGLLSEK
                     GITTRDGLADLAVDELVELTGLPPERSQDLIMKARAHWFENTEHAE"
     gene            complement(3493..>3689)
                     /locus_tag="VHT22_01310"
     CDS             complement(3493..>3689)
                     /locus_tag="VHT22_01310"
                     /inference="COORDINATES: protein motif:HMM:NF036577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX3136216.1"
                     /translation="RFSGEEVRLTLREAIDNARRLKGILRGVEGDVVRLDTETGPRTI
                     ALANIDRARLVPKIEWRNAR"
BASE COUNT          477 a         1282 c         1123 g          807 t
ORIGIN      
        1 cggccagctg ctcgaacatg ttctcgagct tcgccgcctg ctgcttggct agcttgacgt
       61 cacggaactt gccctgccgg aacagcgcga tctcgcgtgc cttgcgctcg tcgccgagca
      121 ccatcacgtc ctcgcccgcc agcggcacgt ccgacaggcc ctggatctcc accggaatcg
      181 ccggaccggc agaggctacc gtcttgccgg tctcgtcggt catcgcgcgc actcgcccga
      241 acaccgcgcc ggcgagcacg atatcgccgc gcttcaaggt gcccgaattc accagcaccg
      301 tggccaccgg gcccttgccc ttgtcgaggc gtgattcgat gacgatgcct ttggccggcg
      361 catccttcgg cgccttgagc tccagcactt cggcctgcag caggatcgcg tcgagcaaat
      421 tgtcgatgcc ggcgccggtc ttcgccgaga cctggacgaa ctgcgtgtca ccgccgtatt
      481 cctcgggcag cacgctttcg gcgacgagct cctgcttgac ccgatccgga ttggcttcgg
      541 gcttgtcgat cttgttgatc gcgacgacga tcggcacgtt gccagccttc gcgtgcgcga
      601 tcgcttcctt ggtctgcggc atcacgccat cgtcggccgc aacgaccagg atgacgatgt
      661 cggtcacctt ggcgccccgg gcgcgcattg ccgtgaacgc ctcgtggccc ggcgtgtcga
      721 ggaaagtgat cacgcccttc gccgtttcca cgtggtaggc gccgatatgc tgcgtaatcc
      781 ccccggcttc gccgctggca acacgcgtgc ggcgtatcgt atcgagcagg gacgtcttgc
      841 cgtgatcgac gtgacccatg acggtgacga ccggcggacg cggcagcgat tccgccgtcg
      901 tcgcctgcgc gctttcggcg agcagcgagt ccgggtcgtc gagcttggcc gcgatcgcct
      961 tgtggcccat ttcctcgacg acaatcatcg ccgtttcctg atcgagcatc tgattgatcg
     1021 tgaccattgt gccgagcttc atcatcgcct tgatgatctc ggcggccttg acgctcatct
     1081 tgcgggcaag atccgcgacg gtgatcgtct ccggcaccgc gacttcgcgg atcagcggct
     1141 cgggcgccag cgcctgcgcg ccttcctcga cctcgtcgcc ttcctcgtgc cggtgcttgc
     1201 cgcctttcgg ctgcttccat ccggccgtcg ctgcgccact ggtgacgtcg ccgcgcagct
     1261 tgagcgcgcg ccggcgcgcc gcctcttcct ggaacgcggt tgccgagggc ttgcgtgaag
     1321 gcttgtcttt tttcgcgccg ggcttggcca ccggcttgtg cagcgtgcct tcggtcttcg
     1381 ccttggcttc gggcttcgcc gttgccgcgg gctgcgcgac cggcgccgct tccttggcga
     1441 ccgccgctgc ctcggcttcg gctgccttcg ccagcgcgtc ttcctcttcc ttttcctttt
     1501 tggtcttgcg cttcttcgcc tgttcctgct tttgctgtat ttcttcctgc tgcttggcga
     1561 tcagcgccgc gcccttgcgc gcttcctcct cgcgtgccgc gagctctgtc gcggtgacga
     1621 ccggctgcgg cgcgaccggc acggcctctt ccgtcgccgc tgccgcgtcg gcgccgtcgc
     1681 gcttgacgaa cacccgcttc ttgcggactt cgacctggat cgtgcgcgcg cgaccggtgc
     1741 tgtcggcggc cttgatctcg gtggtctgct tgcgcgtcag cgtgatcttc ttcttctggt
     1801 ccagaccgcc gtgctgcttg cgcaaatagt cgagcagccg ggtcttgtcc tgctcggaca
     1861 gcttgtcgcc ctgtttcttg ccggcgacgc cggcggcggc gagctgctcc agcaggatgc
     1921 ccgccggcat cttgagctcg gtcgcgaatt tatcgatggt ggtctggggc atgttttttc
     1981 tcctgccgcg ctgttccgcc cggccttact ctgcgtgttc cgtgttttcg aaccaatggg
     2041 cgcgcgcttt catgatcagg tcctgcgagc gttccggcgg cagtccggtc aattcgacca
     2101 gctcgtcgac cgcgaggtcg gcgaggccgt cgcgtgtcgt gatgcccttt tcggagagca
     2161 gccccgcggt ctcgttgtcc atgccttcca agttcaacag atcctcggcg acgttcccca
     2221 ccgactcctc acgcacgatc gcctcggtca gcagcgcgtt gcgcgcgcgg ctgcgcaatt
     2281 cgttgacggt gtcctcgtcg aaggcatcga tctcgagcat ctcgttcagc ggcacgtacg
     2341 cgatttcttc gagcgaggtg aatccttcct ggatcaggat atcggccact tcctggtcga
     2401 cgtcgagctt gtcgatgaac agcgcacgcg tgcgctcgct ttcgacctga tgcttctgct
     2461 gcgattcctc gacgcccatc aggttgattt cccatccggt gagctcggaa gccaggcgga
     2521 cattctggcc gttgcgaccg atcgccatcg cgagattttc ctcgtcgacg acgacatcca
     2581 tgctgtgccg ttcctcgtcg acgacgatgc tggagatctc cgccggcgaa agcgcgttga
     2641 taaccagctt tgccgggtcc tcggagaaca ggacgatgtc gacgcgctcg ccggcgagct
     2701 cgttggtcac cgcggtcacg cgcgatccgc gcatgccgat gcaggtgccc tgcgggtcga
     2761 gcctcggatc gttcgacttc accgcgatct tggcgcggac gccggggtcg cgcgccgccg
     2821 ccttgatctc gatcaggcct tcctcgatct ccgggacctc gagctcgaac agccgcacca
     2881 ccaggtcggg cgtgatgcgc gacagcaaca gctgcgggcc gcgcgcctgc gcctcgatct
     2941 tgatcacata tgcccggacg cggtcgccga ctcgcagatt ctccttcggg atcagctgat
     3001 cgcggggaat catgccttcg atccgtcccg attcgacgat cacgttgccg cgctcgatgc
     3061 gcttgaccgt tccggtcagt aggttgtcgt cgcggtccag gaaatcgttc aggatctgct
     3121 cgcgctcggc gtcgcggatc ttctgcagga tcacctgctt cgccgcctgc gctccgatgc
     3181 ggccgaattc gacaggctcg agcggttcct cgatcacgtc gcccagggcg agcggcggat
     3241 cgcgctcggc ggcgtcggtg atcgcgatct gccgcgccgg ctcctcgtgc tcctcgtcgg
     3301 gcacgaccgt ccaccgccgg aacgagtcgt agtcgccggt ttcccgatcg atcgacacgc
     3361 gggcatcgat atcctgggta aagcgtttct tggtcgctga cgccagcgcc gactccagcg
     3421 ccgtgaacac gatatctttc ggcacgtttt tctcgcgcgc cagcgcgtcg accagcaaca
     3481 gcaattcgcg gttcatcttg cgtttctcca ctcaatcttc ggcaccagcc gcgcgcggtc
     3541 gatattggcc agcgcgatcg tgcgtggacc cgtctctgtg tccagccgca ccacatcgcc
     3601 ttcgacgccg cgcagaatgc ctttcaagcg acgggcgttg tcgatagcct cgcgcaaagt
     3661 aagtcgcact tcctcgcccg agaaacggg
//
LOCUS       DATANS010000088         6394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_256350, whole genome shotgun sequence.
ACCESSION   DATANS010000088 DATANS010000000
VERSION     DATANS010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256350"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="VHT22_01315"
     CDS             complement(<1..170)
                     /locus_tag="VHT22_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEX3136217.1"
                     /translation="MNAAQRLHALGQSLWLDNLSREILDDGTIARYIREFALTGLTSN
                     PTIFDKAIRKTSR"
     gene            250..1686
                     /gene="zwf"
                     /locus_tag="VHT22_01320"
     CDS             250..1686
                     /gene="zwf"
                     /locus_tag="VHT22_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012409487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX3136218.1"
                     /translation="MLKLDSGMPGPFGGGKALSNATGADALVLFGGTGDLARKKIYPA
                     LLAMARRGHLGIPVIAVARAPGDASTLRAIVHDSLQQQGLVDDAAFAKLSPLLNYVRG
                     DYGEVATYDAIRRGLGSSVRPLYYLAIPPDAFPVVVAALGRMGDTEQARVVIEKPFGR
                     DLESARSLNRTLHQVFREPMIFRIDHYLGKEAIQNLYYFRFANAFLEPVWNRNYVANV
                     QITMAERFGVEGRGEFYESVGALRDVVQNHLLQVTALLAMEPPYSHRGDAQRDEKAKV
                     LRAVRPLAASDIVRGQYRGYRAEKGVTPDSQVETFAAMRLHVDSWRWAGVPFLIRAGK
                     RLPVTATEVIVELKRPPQNVVDEPLPPGANYVRFRLGPKRVAIAIGVRSKLPGTALVG
                     RDLELYCCNEQDDAMDAYERLIGDALRGDSALFARQDTVEAAWCAVEPVLGDSVPVVE
                     YDAGSWGPPQADALIAPFGRWRAPAADS"
     gene            1691..2503
                     /locus_tag="VHT22_01325"
     CDS             1691..2503
                     /locus_tag="VHT22_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX3136219.1"
                     /translation="MAKPSATKAKRASRGKAGRAGPPYTLAIDIGGTHIKASVLDRAG
                     KPIVDRVGVLTPHPAPPAEILELIRAMASALPAFDRISAGFPGVIQGGCVITAPNLGT
                     AAWHRFPLASELESQLGKRARVLNDAEVQGLGVIAGSGLECVLTFGTGMGSAIYRDGQ
                     LMPHLELGQHPVWKNKTYDQYVRAATLKKLGPAKWNRRVSRVITLIRTLLNFDHLYLG
                     GGNAANITLDLPRDVTIVSNLAGITGGVRLWEAQFDDFFVARQPRRAMSRRK"
     gene            2603..2869
                     /locus_tag="VHT22_01330"
     CDS             2603..2869
                     /locus_tag="VHT22_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136220.1"
                     /translation="MSMLLTSEEILDKIRKAFAPYHVVAELQDYHRQLGFRVYDADNE
                     TIDTFEGNLVQDLKNPANLKRMILKARAAIERQQRVLKPWSFEG"
     gene            complement(2906..3901)
                     /locus_tag="VHT22_01335"
     CDS             complement(2906..3901)
                     /locus_tag="VHT22_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase"
                     /protein_id="HEX3136221.1"
                     /translation="MAKIYVIHENDAWVEPLRAAFTELRLPFEEWFLSEGLLDLAAVP
                     PSGVFYNRMSASSHTRGHRFAAEYTAGVLAWLESHRRRVVNDSRALQLEVSKVAQYAA
                     LSRYGIRTPRTFAAVGRAHLIDAARQMPGPFITKHNRAGKGLGVHLFQTAVALEAHLA
                     SPAFEDSVDGVTLIQEYIQAPEPYITRVEFVGGRFLYAVRVDTSLGFELCPADVCQVG
                     DAFCPVGEDANAEGSVLAAVAPRFRIIEDFRHPIVARYRKFIADHGIGIAGIEFITDR
                     AGEIYTYDVNTNTNYNSDAEAAAGIHGMRAIAAYLGSELRRQQRNDGESSTLVAA"
     gene            4072..4509
                     /locus_tag="VHT22_01340"
     CDS             4072..4509
                     /locus_tag="VHT22_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136222.1"
                     /translation="MVYRKIDGLAVRNTGSEAPDSKLGVSSIPKPASKAQSDAQAIAR
                     RRQTPSSDPLPMTFKWIAKLPRSVQPLALLRQYPRIANMMAGIWQDPQSCRAYLHDLL
                     TDRRGTRKGFPSEIVQELVRLRVHYEHLHPPMLDVYKDVNKRG"
     gene            4696..6015
                     /locus_tag="VHT22_01345"
     CDS             4696..6015
                     /locus_tag="VHT22_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3136223.1"
                     /translation="MKPDSDDSGTIQGTATHEDGSTYAWARLAVALVVMTISGASLYV
                     VVVVLPTVQAEFGVARADASLPYTLTTIGFGIGGLFMGRLADRFGVMVPLLIGGLSMG
                     IGFVAAGMADSIWTFALLHGVLIGMFGSSAMFAPLIADTSLWFTRRRGIAVAICASGN
                     YVAGAIWPPVAQHFVATYGWRATYIGIGIFCALSLTMLALLFRRRPPAVEAAEPSAGR
                     GPERASTNARTAAASTGRPFGMSPAAAQALLCVAALSCCIAMAMPQVHIVAYCGDLGY
                     GVARGAQMLAVMLGFGVVSRLLSGVICDRIGGLRTLLLGSALQAVALLLFLPFDGLVS
                     LFVISALFGLFQGGIVPSYAIIIREHFPPTEAGARVGMVLMFSLFGMALGGWLSGKVF
                     DLTGSYQAAFVNGIGWNLLNLAIALMLLHRARGRRARAPDAAAGIAF"
     gene            6058..>6394
                     /locus_tag="VHT22_01350"
     CDS             6058..>6394
                     /locus_tag="VHT22_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX3136224.1"
                     /translation="MMFQRSDDRFLAEIRSRLYVSVVSDVLDALGHTEQAMAARIRPL
                     DDALVLVGYARTGVYNDVYHVAKGENPYELEIALIDDLARDEVPVFACGASGRIAPWG
                     ELLSTAARAR"
BASE COUNT         1055 a         2007 c         2131 g         1201 t
ORIGIN      
        1 cggctcgtct tccggatggc cttgtcgaag atcgtcggat tggatgtgag gccggtcagc
       61 gcgaactccc tgatgtacct ggctatcgtg ccgtcgtcca ggatttcgcg tgacagattg
      121 tcgagccaga ggctctggcc gagcgcgtgc agccgttgcg cagcattcat gcttgcctcc
      181 ttgttcggcc gacgtccggc tatgcgtcgg aaccaacgat agcagcgcgc cgtctgaccg
      241 ggcgtgacca tgcttaaatt ggacagcggg atgccggggc cgttcggcgg cggaaaagcc
      301 ctgagcaacg ccacgggggc cgacgcattg gtgcttttcg gcggtaccgg cgatctcgcg
      361 cgcaagaaga tctatccggc gctgctcgcg atggcgcgcc gtggccatct cggcattccc
      421 gtgatcgccg tcgcgcgcgc gccgggcgac gcgtcgacgc tgcgcgcgat agtgcacgat
      481 agtttgcagc agcaagggct ggtggacgac gcggcgttcg cgaagcttag tccgctgctc
      541 aattacgtcc ggggcgatta cggcgaagtc gcgacttacg atgcgattcg tcgcgggctc
      601 ggctcgtcgg tgcgcccgct ctactacctg gcgattcctc ccgatgcatt tccggtcgtc
      661 gtggccgcgc ttggccgcat gggcgacacc gagcaggcgc gcgtcgtcat cgaaaagccg
      721 ttcggccgcg acctcgagtc cgcgcggtcg ctgaaccgga cgctgcatca ggtatttcgc
      781 gagccgatga tatttcgcat cgatcattat ctgggcaagg aggcgatcca gaacctctac
      841 tatttccgct tcgccaacgc gtttctcgag ccggtctgga atcgcaacta cgtggcgaac
      901 gtgcagatca cgatggctga gcggttcggc gtcgaaggcc gcggcgagtt ttacgagtcg
      961 gtcggcgcac tgcgcgacgt cgtgcagaat catctgctgc aggtgacggc gctgctggcg
     1021 atggagccgc cgtacagtca ccggggcgat gcgcagcgcg acgaaaaagc gaaggttctc
     1081 cgcgcggttc gcccgctggc agcgagcgac atcgtgcgcg ggcagtatcg cggctatcgg
     1141 gccgagaaag gtgtgacgcc ggattcgcag gtggagacct tcgccgcgat gcgcctccac
     1201 gtcgattcct ggcgctgggc cggcgtgccg ttcctgattc gcgccggcaa gcgcctgccg
     1261 gtcaccgcga ccgaagtcat cgtcgagctc aagcggccgc cgcaaaacgt cgtcgacgag
     1321 ccgttgcccc caggcgccaa ctatgtgcgg tttcgcctcg ggccgaagcg cgtggcgatc
     1381 gcgatcggcg tgcgctcgaa gctccccggc acggcgttgg tcggccgcga cctcgagctc
     1441 tactgctgca acgagcagga cgacgcgatg gacgcctacg agcggctgat cggcgatgcc
     1501 ttgcgcggcg attcggcgct gtttgcgcgg caggatacgg tggaggcggc atggtgtgcc
     1561 gtcgagccgg tcctgggcga ttccgttccg gtcgtcgagt acgatgcagg aagctgggga
     1621 ccgccgcaag ccgatgcgct gatcgcgccg ttcgggcgct ggcgcgcgcc tgcggcggat
     1681 agctgagcgc gtggccaagc catcggcaac gaaagccaag cgtgcctcgc gcggcaaggc
     1741 cggccgcgcc ggcccgccct atacgctggc gatcgacatc ggcggcacac atatcaaggc
     1801 atcggtgctc gatcgcgccg gcaagccgat cgtcgatcgg gttggcgtgt tgaccccgca
     1861 tcctgcgcca cccgccgaga tactggaact gatacgggcc atggcaagtg cactgccggc
     1921 attcgaccgc atttcggcgg gatttcctgg cgtgatccaa ggtggttgcg tcatcacggc
     1981 gccgaatctg ggcaccgcgg cttggcaccg atttccactt gccagcgagc tcgagagtca
     2041 gctgggtaaa cgcgctcgcg tgctgaacga cgccgaagtg cagggcttgg gcgtaatcgc
     2101 gggttcgggc ctcgaatgcg tgctcacctt tggcaccggc atgggcagcg cgatctatcg
     2161 cgacggccag ctgatgccgc atctcgaact gggacagcac ccggtgtgga agaacaagac
     2221 ttacgaccag tacgtgcgcg cggcgaccct gaagaaacta gggccggcca agtggaaccg
     2281 gagagtaagc cgcgtgatta cactcatccg gacgctgctc aatttcgatc acctctatct
     2341 gggcggcggc aacgccgcca atatcacgct ggacttgccg cgcgatgtga ccatcgtatc
     2401 caacctcgcc ggcatcaccg gcggcgtcag gctctgggaa gcgcagttcg acgatttttt
     2461 tgttgcacgc cagccgcgcc gcgcgatgtc gcggcggaag tgagtttttt ccgtgacgac
     2521 gctacactga cgcccgaggc gcgccgcggg gccttgtcgc cttcctgaca tcgccccggc
     2581 tcgcggccca ttgggaggtt tgatgagcat gctgctgacc agcgaggaaa tcctcgacaa
     2641 aatccgcaag gcatttgcgc cgtaccatgt cgtcgccgag ctgcaggatt atcaccggca
     2701 gctcgggttt cgcgtttacg acgccgacaa cgagactatc gacaccttcg agggaaacct
     2761 ggtccaggat ctaaagaatc ctgccaacct gaagcgaatg attctcaagg cgcgcgcggc
     2821 gatcgagcgc caacagaggg tgctcaaacc ctggtcattc gagggttgac cgcccggccc
     2881 gccggcggcg gccgtcgaag ccggctcagg cggcgactag cgtgctcgac tccccgtcgt
     2941 tgcgttgttg ccgtcgtaat tcgctgccca ggtacgcggc gatggcgcgc atgccgtgga
     3001 ttccggccgc cgcctcggca tcgctgttgt aattggtgtt cgtattgacg tcgtaggtat
     3061 agatctcgcc ggcgcgatcg gtgatgaact cgatgccggc gatgccgata ccgtggtcgg
     3121 cgatgaattt ccggtaacgc gcgacgatcg gatgacggaa gtcctcgatg atgcgaaagc
     3181 gcggcgcgac cgcagccaac acgctgcctt cggcattcgc gtcttcgccg accgggcaga
     3241 atgcatcgcc aacctggcag acgtcggccg ggcaaagctc gaagccgagc gaggtgtcga
     3301 cgcgaaccgc gtaaaggaag cggccaccga caaattcgac ccgcgtgatg tacggctcgg
     3361 gcgcctggat gtattcctga atcagcgtga cgccgtcgac agaatcctcg aatgcgggac
     3421 tggcgaggtg agcctcgagt gcgacggcgg tctggaagag gtggacaccc agacccttgc
     3481 ccgcgcggtt gtgcttggtg atgaacgggc cgggcatctg tcgcgcggcg tcaatcagat
     3541 gggcgcggcc gacggccgca aaggtgcgcg gcgtccgaat gccgtatcgc gagagagcag
     3601 catactgcgc gaccttgctt acctcgagtt gcagtgcgcg gctgtcgttg acgacccgcc
     3661 gccgatgcga ttccagccaa gccaggacgc cggcggtgta ttcggccgca aagcgatggc
     3721 cgcgggtgtg cgacgacgca ctcatccggt tgtagaacac gccggacgga ggcaccgctg
     3781 ccaggtccag caagccttcg ctcaggaacc attcctcgaa cgggaggcgt agctcggtga
     3841 acgccgcccg caatggctct acccaggcgt cgttttcgtg gatgacgtag attttggcca
     3901 ttttttcact gtcgatcagc tgacaaggct atggcgagtc gcgaaagact catcgtacgt
     3961 agaggtcgcc gccgcgtgaa agaaccttcc ggcacatgct tgtaaccaac agtaatagcc
     4021 cgactcgaat ttcgcggcga acgcggtgcg ttttttgctt caatgcctgt gatggtctac
     4081 cgaaaaatcg acggtctagc tgtacgcaac accggttcgg aagcgccgga ctcgaagctg
     4141 ggcgtctcga gcatcccgaa acccgcgtcc aaggcccagt ccgacgcgca ggcgatcgcg
     4201 cggcgcaggc aaacgccgag cagcgacccg ctgccgatga cgttcaaatg gattgccaag
     4261 ctgccgcgca gcgtccagcc gcttgccctt ttgcgtcaat acccgcgcat cgccaacatg
     4321 atggccggca tctggcagga cccgcaatcg tgccgcgcgt atctgcatga cctcttgacc
     4381 gatcgccgcg gcactcgcaa gggatttccg tcggagatcg tgcaggagct ggtcaggctg
     4441 cgcgtccact acgagcacct gcacccgccg atgcttgatg tgtacaagga tgtgaacaag
     4501 cggggctaaa acccttggtc gactgcgacg gcccatcgcc ggactcgacc ctacgcttgc
     4561 cggcggtcac tgcagcatcc gacatgccgc gcctcaagcg acggccaccc aaacgcgtgt
     4621 gttaagtttc gtctggccct tccttagccg cgcatcggcg cagcgacccg atttgtcgac
     4681 gcgcccgacc gaatgatgaa accagacagc gacgactccg gtacgatcca gggcaccgcc
     4741 acgcacgagg atggctcgac ctacgcgtgg gcgcggctcg cggtggcgct ggtcgtgatg
     4801 accatcagcg gcgccagctt gtacgtcgtc gtggtcgtgt tgccgaccgt gcaggcggaa
     4861 ttcggcgtcg cgcgcgccga tgcctcgctg ccgtacacgc tgacgacgat cggctttggc
     4921 atcggcgggc ttttcatggg ccgactggcc gatcgtttcg gcgtgatggt gccgctgttg
     4981 atcggcggct tgagtatggg catcggtttt gtcgctgccg gtatggcgga cagtatctgg
     5041 acgttcgcgc tgctgcacgg cgtgctgatc ggcatgttcg gaagctcggc gatgttcgcg
     5101 ccgctgatcg ctgacacctc gctctggttc acccggcgcc gcggcatcgc ggtcgcgatc
     5161 tgcgccagcg gcaactacgt cgcgggcgcg atctggccgc cggtggcgca gcattttgtc
     5221 gccacgtacg gctggcgtgc gacctatatc ggaatcggga tcttctgcgc gctgtcgctg
     5281 acgatgctgg ctttgttgtt caggcgccgg ccgcctgcgg tcgaggcggc tgagccttcc
     5341 gccgggcgcg gtccggaacg cgcttcgacg aacgcgcgga ccgctgcggc atcgacgggg
     5401 cgaccgttcg gcatgagtcc ggccgctgcg caggcgctgc tatgcgtcgc cgcactctcc
     5461 tgttgcatcg cgatggcgat gccgcaggtg catatcgtcg cctactgcgg cgatctgggc
     5521 tacggcgtcg cgcgcggggc acagatgctg gcggtcatgc tcggtttcgg cgtcgtcagc
     5581 cggcttttgt ccggcgtgat ctgcgaccga atcggcgggc tgcgcacctt gctgctggga
     5641 tcggcgctgc aggcagtcgc gctgttgttg ttcctgccgt tcgatgggct cgtatcgctg
     5701 ttcgtgatct cggcgctgtt cggcctgttc cagggcggga tcgtgccttc gtatgcgatc
     5761 ataatccgcg agcattttcc accgaccgaa gccggtgcgc gggtcggcat ggtgctgatg
     5821 ttctcgctgt tcgggatggc gctcggcgga tggctgtcgg gcaaggtgtt cgacctgacc
     5881 ggctcgtatc aggcggcgtt cgtcaacggc atcggctgga acctgttgaa tcttgccatc
     5941 gccttgatgc tgctccatcg cgcacgcggc cgtcgcgctc gtgcgccgga tgccgcggcc
     6001 ggcattgcat tctgaccgcc ggcgtccaca tcgattgcct tcgacgggag tgaaccgatg
     6061 atgttccagc gaagcgacga ccgatttctt gccgaaatcc gcagccggct ctatgtctcg
     6121 gtcgtgtccg atgtgctcga tgcgctcggc cacaccgaac aggcgatggc cgcgcgtatc
     6181 cgaccgctgg acgatgcgct ggtactcgtc ggctacgcca gaaccggcgt gtacaacgac
     6241 gtctaccacg tcgcgaaggg cgaaaatccg tacgagctgg aaatcgcact gatcgacgat
     6301 ctggcgcgtg acgaggtccc ggtgttcgcc tgcggcgcct cggggcggat cgcgccatgg
     6361 ggcgagctcc tatcgaccgc ggcgcgggcg cgag
//
LOCUS       DATANS010000089         2261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_802064, whole genome shotgun sequence.
ACCESSION   DATANS010000089 DATANS010000000
VERSION     DATANS010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802064"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2261)
                     /locus_tag="VHT22_01355"
     CDS             complement(<1..>2261)
                     /locus_tag="VHT22_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX3136225.1"
                     /translation="RLFTDNIPEPVVYVDLDRHYTFVNDAFVSLVGISRDEIIGKSVK
                     EVLGPEVFEFQQPFIDRAARGESVTYERETIDVNGRQRWLRNKIVPDMRFDATIKGYY
                     IVGHDITDLKQAQDALASRESQLRAIMDGVPAPVAYIDRDERCQYVNRPFLQYFGLTA
                     QQVAELRLRDVVGHGIYASAQALLSRALQGESTSFERLVPGADAVKRWMTIRVVPDTA
                     PGGVVNGAFVLMNDIHGLKQAQEALRASEGELRLIMDNVPARVAYIDRDSCFRFLNRH
                     NEEWLSESRKDLTGRKIGEVVGDARARQLQPLMNRVFSGETISTEQMLVQPNGEQRWE
                     SIHYAPNRDGEGNVIGIYAVHTDIHDQKRNEEALRRANWMLSSHINNTPLAVLEWDRD
                     FRLVRWSPQAENIFGWRAEEVLGMPITGSQLTHEADRPGVTELIEKLMAGEQPRATGL
                     NRNYRKDGETIWCEWYHSCLLDEQGGIVSILSFVQDVSSRIQAEERLQYLATRDALTG
                     LPNRLLLQERLAQAIAQAKRNGRNVGVLYIDLDRFKNVNDTLGHRIGDELLKQVTKSL
                     SGALRETDLLARLGGDEFMVIVEDFDDAAVLNRVAQKLQDAIAQPFQIEEHDIYVTSS
                     IGISIYPDDSDDPEGLLKHADVAMYRSKELGRNTYQFFDADLAARRLKQHTLESSLRT
                     AVKECALELHFQPIVRIADRSIVGAEALLRWHDEEHGEIAPQVFIPLAEESGLIHVLG
                     DWVLKTAAEQCAA"
BASE COUNT          366 a          757 c          710 g          428 t
ORIGIN      
        1 cgccgcgcac tgctcggcgg cggttttcag gacccagtcg ccgaggacgt ggatcagccc
       61 cgactcttcg gcaagcggaa tgaaaacctg cggcgcgatt tcgccgtgct cctcgtcgtg
      121 ccagcgcagc agcgcctcgg cgccgacgat cgacctgtcg gcgatgcgca cgatgggctg
      181 aaagtgcagc tcgagcgcgc attccttgac tgcggtgcgc aacgagcttt ccagcgtgtg
      241 ctgcttcagc cgtcgcgccg ccagatcggc atcgaaaaac tgataggtat tgcgtcccag
      301 ctccttcgag cggtacatcg cgacgtcggc gtgcttgagc aggccctcgg ggtcgtcgct
      361 gtcgtcagga tagatcgaga tcccgatcga cgaggtcaca tagatgtcgt gctcctcgat
      421 ctggaacggc tgcgcgatcg cgtcctgcag cttctgcgcg acgcgattca acaccgccgc
      481 gtcgtcgaag tcctcgacga tgaccatgaa ctcgtcgccg ccaagccgcg ccagcaggtc
      541 ggtttcgcgc agcgcgccgg acagcgactt ggtcacctgc ttcaacagct cgtcgccgat
      601 ccggtgtccc agcgtgtcgt tgacgttctt gaaccggtcg agatcgatgt agagaacgcc
      661 gacgttgcgg ccgttgcgct tagcctgagc gatcgcctgc gccagccgct cctgcagcag
      721 cagccggttc ggcaacccgg tcagcgcatc gcgcgtggcc aggtactgca ggcgctcctc
      781 cgcctggatg cgggacgaga cgtcctgcac gaacgacagg atcgacacga tgcctccctg
      841 ctcgtcgagc aggcaggagt gataccactc gcaccagatc gtctcgccgt ccttgcgata
      901 gttgcggttg agtccggtcg cacgcggctg ctcgccggcc atcagctttt cgatcagctc
      961 ggtcacccca gggcgatctg cctcatgcgt cagctggctg ccggtgatcg gcatgcccag
     1021 cacttcctcg gcccgccatc cgaatatgtt ctcggcctgc ggcgaccagc gcaccaggcg
     1081 gaaatcgcga tcccattcga gcaccgccag cggcgtgttg ttgatgtgcg acgacagcat
     1141 ccagttggcg cggcgcaggg cttcctcgtt gcgcttctgg tcgtgaatgt cggtgtgcac
     1201 cgcatagatg ccgatcacgt tgccttcgcc gtcgcggttg ggcgcgtaat gaatcgactc
     1261 ccaccgttgc tcgccgttcg gctgcaccag catctgctcg gtcgagatcg tctctccgga
     1321 gaacacccgg ttcatcagcg gttgcagctg ccgcgcgcgg gcgtcgccga cgacttcgcc
     1381 gatctttcgc ccggtcaggt ccttgcggct ttccgacagc cattcctcgt tgtgccggtt
     1441 gaggaagcga aagcacgaat cccggtcgat gtaggcgacg cgggcgggaa cgttgtccat
     1501 gatcagccgc agctcgccct cgctggcgcg cagagcttcc tgcgcctgct tcaggccgtg
     1561 gatgtcgttc atcagcacga acgcgccgtt gaccacgcct cccggcgcgg tatccggcac
     1621 gacgcggatc gtcatccacc gcttgaccgc atccgcaccg ggcaccagtc gctcgaacga
     1681 ggtcgattca ccctgcagcg cgcgcgacag caacgcctgc gcgctggcat agatgccgtg
     1741 gccgacgacg tcgcgcaggc gaagctcggc aacctgttgc gcggtcaggc cgaaatactg
     1801 caggaacggc cggttgacat actggcagcg ctcgtcgcgg tcgatatagg caaccggcgc
     1861 cggcacgccg tccatgatcg cgcgcagctg cgattcccgc gaagccagcg cgtcctgcgc
     1921 ctgcttgagg tcggtgatgt cgtggccgac gatgtaataa cccttgatcg tcgcgtcgaa
     1981 acgcatgtcc gggacgatct tgttgcgcag ccaccgctgg cggccgttga cgtcgatggt
     2041 ctcgcgctcg taggtgacgc tctcgccgcg ggcggcgcgg tcgatgaacg gctgctggaa
     2101 ctcgaatacc tccggcccca gcacctcctt cacgctcttg ccgatgatct cgtcgcggct
     2161 gatacccacc aggctgacaa aagcgtcgtt gacgaacgtg tagtgtcggt ccaggtcgac
     2221 gtacacgacc ggctcgggaa tgttgtcggt gaacagccgc a
//
LOCUS       DATANS010000090         2268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_718040, whole genome shotgun sequence.
ACCESSION   DATANS010000090 DATANS010000000
VERSION     DATANS010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718040"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="VHT22_01360"
     CDS             complement(<1..1261)
                     /locus_tag="VHT22_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX3136226.1"
                     /translation="MTTIKQQDLIQSVADALQYISYYHPVDYIEALAGAYAAEESPAA
                     KDAIAQILTNSRMCAEGHRPICQDTGIVVAFVKVGMDVRWEGATMSATEMINEGVRRA
                     YLDPDNKLRASIVDDPAGARRNTRDNTPAVVHFELVPGNKVDVRIAAKGGGSENKAEF
                     VMLNPSDSIVDWVVRTLPTMGAGWCPPGMLGIGIGGSAEKAMLLAKEALMQPIDMAML
                     KKRGPANPLEQLRIDIYDAANALGIGAQGLGGLSTVLDVKILDYPTHAASKPVGMIPN
                     CAATRHAHFTLDGSGAASLSPPDLARWPDVRWAPSPTAKRVDLDRLTREEVASWRPGD
                     RLLLSGKLLTGRDAAHKRIADLFARGESLPPGVDFTNRVIFYVGPVDPVRDEVVGPAG
                     PTTATRMDKFTELMLAKTGLLAMIGKAE"
     gene            1515..>2268
                     /locus_tag="VHT22_01365"
     CDS             1515..>2268
                     /locus_tag="VHT22_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3136227.1"
                     /translation="MSTIESVSNETRVFEPPRDFVAQANVTRTDFDRLNAAAAADYSG
                     FWAKLARENLLWHKPFTKSLDESNAPFYKWFEDGELNVSHNCLERNLENGNANKVAII
                     FEADDGAVTRITYQELYHRVCRFANGLKSLGIKKGDRVIIYMPMSIEGVVAMQGCARI
                     GATHSVVFGGFSAKSLQERIIDVGAVAVVTADEQARGGKPLPLKTIVDEALGMGGCEA
                     VRKVIVYRRTGGKVPFAAPRDLWLHELVAAQPD"
BASE COUNT          398 a          740 c          730 g          400 t
ORIGIN      
        1 gctcggcctt gcctatcatc gccagcaggc cggtcttggc cagcatcagc tcggtgaact
       61 tgtccatccg tgtcgccgtc gtcggtccgg ccgggccgac gacttcgtcg cgcaccggat
      121 cgaccggacc gacgtaaaag atgacgcgat tggtgaagtc aacgcccggc gggagcgatt
      181 cgccgcgcgc gaacagatcg gcgatccgct tgtgcgcggc gtcgcgcccg gtcagcagct
      241 tgccggacag cagcagccga tctcccggtc tccaactcgc tacctcttca cgcgtcagcc
      301 ggtccaagtc gactctcttg gccgtcggcg acggcgccca gcgcacatcg ggccagcgcg
      361 caagatccgg cggggagagg ctcgcggcgc ccgacccgtc gagcgtaaag tgcgcgtgcc
      421 gcgtcgctgc gcagttcggg atcatgccga ccggcttgct ggcggcatgc gtcggataat
      481 cgagaatctt gacatcgagc accgtcgaca gcccgcccag cccttgcgcg ccgatgccga
      541 gtgcattggc ggcgtcgtag atgtcgatgc gcaactgctc gagaggattg gcgggaccgc
      601 gcttcttgag catggccatg tcaatcggct gcatcagcgc ttccttggcc agcagcatcg
      661 ccttttcggc gctgccgcct atgccgatgc ccagcatgcc aggcgggcac cagccggccc
      721 ccatcgtcgg caaggtccgc accacccagt cgacgatcga gtccgacggg ttgagcatca
      781 cgaattccgc cttgttttcg gaaccgcccc ccttggcggc gatccggaca tcgaccttgt
      841 tgccgggcac gagctcgaag tgcaccacgg ccggcgtgtt gtcgcgcgtg ttccggcgcg
      901 cgcctgccgg gtcgtcgacg atcgatgcgc gcagcttgtt gtccggatcg agataagcgc
      961 ggcgcacgcc ttcgttgatc atctcggtcg ccgacatcgt cgcaccttcc caacgcacat
     1021 ccatgccgac cttgacgaac gcaacgacga tgccggtgtc ctggcaaatc ggccgatggc
     1081 cttcggcgca catgcgcgaa ttggtaagaa tctgcgcgat cgcgtccttg gcagccggcg
     1141 attcctcggc cgcataggcg ccggcaagcg cctcgatgta gtcgaccggg tggtaatagg
     1201 aaatatattg cagcgcgtcg gctacactct ggatcaggtc ttgttgcttg atcgtcgtca
     1261 tggctggtgg tcgacgcaaa cggcgggcga aacgcccggc aaagcggacg gtcaggtcat
     1321 gcgattctac catcgcggga ccctgatccg gctccgcgcg accaccgcag gatgccgcgt
     1381 gtcgtcgccg cgtgcggttg ccgcgtgtac ttgccaattg caccgtaagg gtaagactcg
     1441 gtagactgcg gtggagcgca caggagcgcg aggggatcct gtcccttcgt ccgcgccatc
     1501 taggagcagg caacatgtcg acgatcgaat cggtatcgaa cgaaacgcgc gtattcgagc
     1561 cgccgcgcga ttttgtcgcg caggcgaacg tcacccggac cgacttcgat cggctgaacg
     1621 ccgccgccgc ggccgattac tccggcttct gggcgaagct cgcacgcgag aacctgctct
     1681 ggcacaagcc gttcaccaag tcgttggacg agagcaatgc accgttctac aagtggttcg
     1741 aggacggcga gctcaacgtc tcgcacaact gtctcgagcg caatctagaa aacggcaacg
     1801 cgaacaaggt cgcgatcatc ttcgaggccg acgacggcgc cgtgacgagg atcacctatc
     1861 aggagctcta ccaccgcgtc tgccgcttcg ccaacggtct caagtcgctc ggcatcaaga
     1921 aaggcgatcg cgtcatcatc tacatgccga tgtcgatcga aggggtcgtc gcgatgcaag
     1981 gctgcgcgcg catcggggcc acgcattcgg tagtctttgg cggattttcg gccaagtcgc
     2041 tgcaggagcg catcatcgac gtcggtgcgg tcgccgtcgt caccgcggac gagcaggcgc
     2101 gcggcggcaa gccgctgccg ctgaaaacga tcgtcgacga ggcgctcggc atgggcggat
     2161 gcgaagcggt gcgcaaggtg attgtctacc gccggaccgg cggaaaagtt ccctttgcgg
     2221 cgccgcgcga cctgtggctg cacgagctgg tggcagcgca gcccgaca
//
LOCUS       DATANS010000091         1634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_509153, whole genome shotgun sequence.
ACCESSION   DATANS010000091 DATANS010000000
VERSION     DATANS010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509153"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..870)
                     /gene="pgeF"
                     /locus_tag="VHT22_01370"
     CDS             complement(130..870)
                     /gene="pgeF"
                     /locus_tag="VHT22_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX3136228.1"
                     /translation="MSAAADWIVPDWPAPARVRSLITTRRGGVSTGAHASFNLGLRAD
                     DDPHAVAANRAALREHLPQDPVWLKQVHGARVVRADAVNDSPEADAAYTRRAETVCAV
                     MVADCMPVLLCDTQASVVAIAHAGWRGLSAGVIENTIAAMGAKANDLLAFLGPAIGPT
                     AFEVGADVRDAFLAADAAAAAAFTPLREGKWLADVFLLGGQRLARAGVHRIYGGGLCT
                     VSDPARFYSYRRDRMTGRMAALIWLANA"
     gene            complement(867..>1634)
                     /locus_tag="VHT22_01375"
     CDS             complement(867..>1634)
                     /locus_tag="VHT22_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX3136229.1"
                     /translation="TKVWGGEEVVVQPVADPAASAATAEAIALDVVHEDAAVLIINKP
                     AGLVVHPGRGNWSGTLLNALLAHAPQLAILPRAGIVHRLDKETSGLLVVAKSLEAQTS
                     LVRQLQARTVRREYLTMVHGRVARGGKVEAPIGRHPKQRTRMAVAARGRPSITHYEVL
                     RHFAHATLLRCKLETGRTHQIRVHMQSLGHPLVGDRVYGKKRTADPLLGAFRRQALHA
                     ERLEFIHPASRAAVEWQAPPPADMRALIAALEAQVSA"
BASE COUNT          272 a          556 c          553 g          253 t
ORIGIN      
        1 cgcgacgctc aacaggccgc cggcgaaact ggtggcgacg atccaggcaa gaacggacat
       61 ggcggcgcga aatgacactg agacgaaagg cgcgaattat aaacgacgcg ccgcggatgc
      121 cgctatttct caggcattag caagccagat caacgccgcc atgcggccgg tcatgcgatc
      181 gcgccgatac gaataaaaac gcgccgggtc gctgaccgta cataacccgc cgccgtaaat
      241 gcgatgcacg ccggcgcggg cgagacgctg gcccccgagc agaaacacgt ccgccagcca
      301 ttttccttct cgcagcggcg tgaaagcggc agccgcagcg gcgtcggcgg caagaaacgc
      361 gtcgcgcacg tcggcaccga cttcgaaagc cgtcggcccg atcgccgggc cgaggaatgc
      421 gagcaggtcg ttcgccttgg cgcccatcgc cgcaatcgtg ttctcgatca cgcccgcgct
      481 caagccgcgc cagccggcat gtgcgatcgc cacgacgctc gcctgcgtat cgcacaacag
      541 taccggcatg cagtcggcga ccatgaccgc gcacaccgtt tcagcgcggc gcgtgtaagc
      601 agcgtcggcc tcgggcgagt cgttgactgc atcggcgcgc acgacgcgcg cgccatgcac
      661 ttgcttcagc cagacgggat cctggggcag gtgctcgcgc aacgcggcgc gattcgccgc
      721 caccgcgtgc gggtcatcgt ctgcgcgcaa gcctaggtta aagctcgcgt gcgcgccggt
      781 gctgacaccg ccccggcggg tcgtaatcag gctgcgcact cgcgcgggcg ccggccagtc
      841 cggcactatc cagtcggccg cggcgctcac gcggacacct gcgcttcgag cgccgcgatc
      901 agcgcgcgca tatcggcggg cggcggcgcc tgccactcga cggccgcgcg actggccgga
      961 tgaatgaatt cgaggcgctc ggcatgcaat gcctgacgcc ggaacgcgcc gagcagcgga
     1021 tcggcggtgc gtttttttcc gtatacgcga tcgccgacca gcggatggcc gagcgactgc
     1081 atatgcacgc ggatctggtg cgtgcgcccg gtttcgagtt tgcagcgcaa taacgtcgca
     1141 tgggcaaaat ggcgcagcac ttcgtaatgg gtgatggaag ggcggccgcg cgcggccacc
     1201 gccatgcgcg tgcgctgttt tggatggcgg ccgatcggcg cttcgacctt gccgccgcgt
     1261 gcgacccggc cgtgaaccat ggtgaggtac tcgcgcctga ccgttcgcgc ctgcagctgg
     1321 cgcacgagac tcgtctgggc ttcgagcgac ttggcgacga ccagcaagcc gctcgtttcc
     1381 ttgtcgagcc ggtgcacgat ccccgcgcgc ggcaggatcg cgagttgcgg cgcgtgggcg
     1441 agcagcgcgt tgagcagcgt gccgctccag ttgccgcggc cgggatggac gacgagcccg
     1501 gccggtttgt tgatgatgag cacggccgcg tcttcgtgca ccacgtcgag cgcgattgct
     1561 tccgcggtcg cggcactggc tgccggatcg gcaaccggct gcaccaccac ttcttcgccg
     1621 ccccatactt tggt
//
LOCUS       DATANS010000092         2014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_214520, whole genome shotgun sequence.
ACCESSION   DATANS010000092 DATANS010000000
VERSION     DATANS010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214520"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1616)
                     /locus_tag="VHT22_01380"
     CDS             complement(<1..1616)
                     /locus_tag="VHT22_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX3136230.1"
                     /translation="MEAQPAGHKSRVGPFHGSADALALAELAGKVRPLLVITATAGAA
                     QRLLEEIPYFNPALKVHLLPDWETLPYDTFSPHHDLVSERLATLYQITQQAFDIAIVP
                     VTTALYRLTPIEYLAARTFFFKQGEKLAADELRKQLTVAGYTHVAQVLSPGEYSFRGG
                     LIDLFPMGSALPYRIDLFDDQIESIRSFDVDTQRSIYKVNEVRLLPAREFPLDEDGQT
                     MFRRNFRTRFEGDPSRSRLYKDVSNGIAPAGVEYYLPLFFEHTALLTDYLPAHTTVVL
                     HSDVQPAIEQFWQDTQARFDLLGGDRSQPLLPPRDLFLTTDAFFGAIKPFSRVEISAV
                     ERPEEIASHLTPHASPATAPLPPVAVDRRADDPLSKLQAFIAGYDGRVLLVAEALGRR
                     ETMLEYFREHGILPAPCASYAEFLAGDAPVMLGVSPLAAGFLLAGQRIAIITENELYA
                     SQVRPRREREGRKTSTEGMLRDLSEVKVGDPVVHLQHGIGRYLGLQNLDFGEGATEFL
                     TLEYTRGDKLYVPVSQLHLISRYSGAPAETA"
     gene            1772..>2014
                     /locus_tag="VHT22_01385"
     CDS             1772..>2014
                     /locus_tag="VHT22_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX3136231.1"
                     /translation="MPRLWSTVLPVLLAFCTAGALAQSYPTRAIKLVVPSSPGGGTDI
                     VARILGQKLSEQLGQQFVVENRAGAGTVIGNDAVAKS"
BASE COUNT          363 a          593 c          676 g          382 t
ORIGIN      
        1 gccgtttcgg cgggcgcgcc gctgtagcgg ctgatcaggt gcaattgcga taccggcacg
       61 tacagcttgt cgccgcgcgt gtattcgagc gtcaggaact cggtcgcgcc ttcgccgaaa
      121 tcgagattct gcagaccgag gtaacggccg atgccatgtt gcagatggac gacggggtcg
      181 ccgactttga cctcggacag gtcgcgcagc atgccttcgg tgctggtctt gcgaccctcg
      241 cgctcgcgcc gcgggcgcac ttgcgacgca tagagctcgt tttcggtgat gatcgcgata
      301 cgctgtccgg cgagcaagaa accggcggcg agcggcgaca cgcccagcat caccggtgcg
      361 tcgccggcaa gaaattcggc gtagctcgcg cacggcgccg gcaggatgcc gtgttccctg
      421 aaatattcga gcatcgtctc gcggcggccg agcgcttcgg cgacgagcag cacgcggccg
      481 tcgtagcccg cgataaacgc ctgcagtttg ctcagcggat cgtcggcgcg gcggtcgacc
      541 gccaccggcg gcagcggggc cgttgccggt gaggcgtgag gggtgaggtg tgaggcgatt
      601 tcctcggggc gctccactgc gctgatctcc acgcgcgaaa acggtttgat cgcgccgaaa
      661 aatgcatcgg tcgtcaggaa caagtcccgc ggcggcaata gcggctggct gcgatcgccg
      721 cccaggagat cgaagcgcgc ctgcgtgtcc tgccagaact gctcgatcgc gggctgtaca
      781 tcgctatgca gcacgaccgt cgtgtgcgcc ggcaggtagt cggtcaggag cgcagtgtgc
      841 tcgaaaaaga gcggcaggta gtattcgaca ccggcgggcg cgatgccgtt gctcacgtcc
      901 ttgtacagcc gtgaccgcga cggatcgcct tcgaagcgcg tgcggaaatt gcggcggaac
      961 atcgtttgcc cgtcttcgtc gagcggaaat tcgcgcgccg gcagcagccg cacttcgttg
     1021 accttgtaaa tgctgcgttg cgtatcgacg tcgaaactgc ggatcgattc gatctggtcg
     1081 tcgaaaagat cgatccggta cggcagcgcg ctgcccatcg gaaacaggtc aatgagtccg
     1141 ccgcggaagc tgtattcgcc gggggaaagg acctgggcga cgtgcgtata gccggcgacc
     1201 gtcagctgct tgcgcagctc gtcggcggcg agcttttcgc cctgcttgaa gaagaacgtg
     1261 cgcgcggcga ggtactcgat gggggtgagg cggtatagcg cggtggtcac cggtacgatc
     1321 gcgatgtcga acgcctgctg cgtgatctgg tacagcgtgg ctaatcgctc ggacaccaga
     1381 tcgtggtgcg gcgaaaaagt atcgtagggc agcgtttccc agtccggcag caaatgcact
     1441 ttgagcgccg gattgaagta ggggatttct tcgagcaggc gttgcgcggc acccgcagtc
     1501 gccgttatca cgagcagcgg acgcaccttt cccgccagtt cggcgagcgc gagcgcatcg
     1561 gccgagccgt gaaacgggcc gacgcggctt ttgtggccgg ccggctgtgc ttccatcaac
     1621 attctgctaa ttgcaaaaaa gcggggatta tacaggcgtt tatctgctcg tttcgtctga
     1681 gtgcggctag gtcagccgaa tccggggctg gtaaagtatc ggtgcatcca cgctcgaact
     1741 tttcctctcg atggtgtaaa gagtgcacat catgccccgc ctttggtcga cagttctgcc
     1801 ggtgttactt gcattctgca cagccggcgc gctggcccaa agttacccca cccgcgcaat
     1861 caaactggtt gttccgtcgt cgcccggcgg cggcactgac atcgtcgctc gtatattggg
     1921 gcagaaactg agcgaacaac tgggacagca gttcgtggtc gagaatcgcg ccggcgcggg
     1981 aaccgtcatc ggcaacgatg cggtcgcgaa aagc
//
LOCUS       DATANS010000093         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_214542, whole genome shotgun sequence.
ACCESSION   DATANS010000093 DATANS010000000
VERSION     DATANS010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214542"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VHT22_01390"
     CDS             complement(<1..423)
                     /locus_tag="VHT22_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136232.1"
                     /translation="MHKLFRSLVCTSSLLCLTMSAAFAAQILGPGSDESDLVPTGKGW
                     GERAYPAPPRANENANGNSNAAGHAKPTSGNGIFYHGGPLMLGTTNAYYIWYGNWSGN
                     TATTILTNLATSIGGSSYFNINTTYYDGSNTAVSNSVHY"
     gene            complement(672..974)
                     /locus_tag="VHT22_01395"
     CDS             complement(672..974)
                     /locus_tag="VHT22_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019392185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136233.1"
                     /translation="MPKLHELAHCRAGDKGDTSILSLIAYRAEDYPLLAERVTADAVR
                     KHLRGIVLGEVRRYELPALWALQFVCDRSLHGGVTTSLALDAHGKSLSYALLEMSV"
     gene            complement(958..>1518)
                     /locus_tag="VHT22_01400"
     CDS             complement(958..>1518)
                     /locus_tag="VHT22_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015374602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX3136234.1"
                     /translation="LYEVTDPFGYVTPDVVADFSTVTMREAGQDRIDVRGATGKARPA
                     KLKVSVGYLAGYVGEGEISYGGANALARARLAGDIVRERLDKAFTDLRIDLIGSTSLH
                     GKSFDPTEQPYEVRLRVAARAPTPESASLIGEEVEALYTNGPAGGGGTRKYVHEQVGI
                     VSTLIERERVPTKVTLREWSRDAQAA"
BASE COUNT          275 a          474 c          484 g          285 t
ORIGIN      
        1 atagtggacg gaattgctta ccgccgtatt cgagccgtcg taatacgtcg tgtttatgtt
       61 gaagtacgac gaaccgccga tgctggtcgc gagatttgtc aggatcgtcg tcgccgtatt
      121 ccccgaccag ttgccgtacc agatgtaata ggcattggtg gtgcccagca tcaacgggcc
      181 gccgtgatag aagatgccgt tgccggacgt gggtttggcg tgcccggcgg cattcgaatt
      241 gccgttggcg ttttcgttcg cccggggcgg cgccggatac gcgcgctcgc cccagccctt
      301 gccggtcgga acgagatccg actcatcgga cccgggtccg agtatctgtg cggcaaaagc
      361 ggcggacatc gtcaggcaca gcagacttga ggtacagaca agggatcgga acagcttgtg
      421 catggaatga gtctcctcga tgtggtgcgc ttcaagcgag cagaggcaat gggcagcgcg
      481 gaattattac aggaactttg gagacagcac cagcacccat ccgacggacg gcgcggttgt
      541 ggccgacaga atgaggctgt cctaaaagct atgttcgcgc cgccgccggg tggtcgccaa
      601 ttcagggagt ggcgtcaacg tgaggaagtg attggcggcg acaagaacgt tctgcgctct
      661 caacgctact actacacgct catttcaagc agcgcgtaac tcaacgactt gccgtgcgca
      721 tcgagcgcca gcgaagtcgt gacgccgccg tgcaacgacc tgtcgcatac gaattgcagc
      781 gcccacaagg ccggcaactc gtagcggcga acctccccca gcacgatgcc ccgcagatgt
      841 ttcctgaccg cgtcggccgt cacccgctcg gccagcagcg gataatcctc cgcccggtat
      901 gcgatcagcg acagtatcga cgtgtcgccc ttgtcgccgg cgcgacagtg cgccagctca
      961 tgcagcttgg gcatcgcgcg accactctct caatgtcacc ttcgtgggca cgcgctcgcg
     1021 ctcgatcaag gtcgagacga tgccgacctg ctcgtgcacg tatttgcgcg tgccgccgcc
     1081 gccggcgggg ccgttggtgt acagcgcttc gacttcctcg ccgatcagcg acgcgctctc
     1141 cggcgtcggc gcacgcgcgg cgacgcgcag gcggacttcg tagggctgct cggtgggatc
     1201 gaacgacttt ccatgcagcg aggtgctgcc gatcaggtcg atgcgcaggt cggtgaatgc
     1261 cttgtccagg cgctctcgga cgatgtcgcc ggcgagccta gcgcgagcca gcgcgttcgc
     1321 gccgccgtag ctgatttcac cctcgccgac gtacccggcg agatagccga cgcttacctt
     1381 gagcttcgcg ggacgcgctt tcccggtcgc accgcgtacg tcgatgcgat cctgtccggc
     1441 ctcgcgcatc gtgacggtgg aaaagtcggc gacgacatcg ggcgtcacat agccgaacgg
     1501 atcggtgacc tcgtagag
//
LOCUS       DATANS010000094         1788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_340119, whole genome shotgun sequence.
ACCESSION   DATANS010000094 DATANS010000000
VERSION     DATANS010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340119"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VHT22_01405"
     CDS             <1..253
                     /locus_tag="VHT22_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136235.1"
                     /translation="AQETERVHLGECKIPIATGRGSGDALFDIVPGTPDESILLHRML
                     STEADVEMPELGRALVHAEGVALIREWIASLGGGCPGPR"
     gene            284..>1788
                     /locus_tag="VHT22_01410"
     CDS             284..>1788
                     /locus_tag="VHT22_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136236.1"
                     /translation="MVTRSSLIWVMTGIAAGIAGGWLWNREHTVEIPAAAGSTLPSEP
                     IRSPFTQRHEERANYLSPSQASSSSKAERESSQPSEFSHRESIYESAKHADRDQLATM
                     IAAARSITNPVERRNTLDVLLLRYAELDIDGALGQALENDPETAAHLLASLTAVAPEQ
                     TWERAKQVTNPAERFTYLQAVVEAWAAQDPERAFSKVADLPAEWQRSELLEAVVTSIA
                     ARDPRQALKLVDSQGPIVAGRLTELIATQWSRTNPSEAARWVEGLSRQDQGRYAYRIA
                     EAYVAQKPSEALAWGLRLAGSPERYLWSSMLGEMAKYDPDQALQIAQSAESPAQRAQA
                     MGKVLAAIAQTNPSLAMTHLMKLPAGGMRSEVLGEVARSVAALTPAQALDWLNDIDEK
                     SLQLEAANGLGWAMSSRNAEAAAQLVDRIPKEARANWITAVALGYASSDVEKGRQWVR
                     RYGNEGAQTATQFARAVASRNPEAAVQLVEDAADDQERDKLLRGLLPPLAE"
BASE COUNT          335 a          571 c          590 g          292 t
ORIGIN      
        1 cgcgcaggag actgaacgcg tccatctcgg cgagtgcaag attcctatcg ccaccggccg
       61 cggctccggc gacgcattgt tcgacatcgt tccgggaact cccgacgagt cgattctgtt
      121 gcacaggatg ctttccacag aagcggacgt agaaatgccg gagctgggcc gagcgctggt
      181 ccatgccgaa ggcgttgcgc tgatccgcga gtggatcgcc tcgctcgggg gcggttgtcc
      241 cgggccccgt tagggcgcag actcgttcac ggaagtcccg tcgatggtga cacgctcctc
      301 gcttatctgg gtcatgaccg gcattgctgc cggcatcgcg ggcggctggc tgtggaatcg
      361 ggagcacacg gtcgagattc ccgctgcggc gggatcgacg ctgccgtccg agccgatccg
      421 ctcgccgttc acgcagcggc acgaagaacg tgcgaattac ctgtcgccgt cgcaagcgtc
      481 cagctccagc aaagcggaac gcgagagctc acagccctcc gaattctcgc atcgcgaatc
      541 gatctatgag tcagccaagc acgctgaccg cgaccagctc gctacgatga tcgccgctgc
      601 gcgttcgatc acgaatcccg tcgaacgacg caacacgctc gacgtactgc tgctgcgcta
      661 tgcggagctc gatatcgacg gcgcgctcgg tcaagcgctc gagaacgatc cggagacagc
      721 cgcacatctg ctggcatcac tcaccgctgt cgcaccggag cagacttggg agcgcgccaa
      781 gcaggtgacc aatcccgccg agcgcttcac ctatctccag gccgtggtcg aagcgtgggc
      841 tgcgcaggac ccggagcgcg cattctccaa ggtcgccgat ctgcctgccg aatggcaacg
      901 cagcgagttg ctggaagcag tcgtgaccag catcgccgcg cgcgatccgc gccaggcgct
      961 caagctcgtg gacagtcagg gaccgatcgt ggcagggcgg ctgaccgagc tgatcgccac
     1021 gcagtggtcg cgaaccaatc cgagcgaagc ggcgcgctgg gtcgaaggac tgtcgcgcca
     1081 ggatcagggt cgctatgcgt atcgaatcgc agaagcctac gtcgcgcaga agccgagcga
     1141 agcgctggcc tggggactgc ggctggccgg ttcgcccgag cgctatctct ggtcgtcgat
     1201 gcttggcgag atggcgaagt acgatcccga tcaagcgctg cagatcgcgc agtcggcgga
     1261 gagtcccgcg cagcgtgcgc aagcgatggg caaggtgctg gctgcgatcg cgcaaaccaa
     1321 cccatcgctg gcgatgacgc atttgatgaa gttgcccgca ggtggcatgc gctctgaggt
     1381 tctcggcgaa gtcgctcgca gcgttgctgc gctgacgccg gcgcaagcgc tcgactggct
     1441 caacgacatc gatgagaagt ccctgcagct cgaagcggcg aacgggctcg gttgggcaat
     1501 gtcgagccgc aacgccgaag cggccgcgca gctcgtcgat cgcattccga aggaggcgcg
     1561 cgccaactgg atcactgctg tggcactcgg ctatgcctcg tccgacgtcg agaaaggtcg
     1621 ccagtgggtg cgacgctacg gcaacgaagg agcccagacc gcgacgcaat tcgcgcgcgc
     1681 cgtggcttcg cgaaatcctg aggctgcagt acaacttgtc gaagacgccg ccgacgatca
     1741 agagcgcgac aaattgctgc gtggtctgtt gccgccgctt gccgagca
//
LOCUS       DATANS010000095         1900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_634871, whole genome shotgun sequence.
ACCESSION   DATANS010000095 DATANS010000000
VERSION     DATANS010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634871"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..>1900
                     /locus_tag="VHT22_01415"
     CDS             134..>1900
                     /locus_tag="VHT22_01415"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136237.1"
                     /translation="MSNDASPATDNALSQAAVDHEHPWLGLASFTEETRAYFYGREEE
                     IAELSRRVQRKLLTILFGQSGLGKTSILRAGIVPRLRPEGYCPVYVRIDYSRDAPEPS
                     EQIKEAIFRATQSLGQWTQTGVAVSGESLWEFLHHRDDVLRDSSGRTLIPLLIFDQFE
                     EIFTLAQSDDFGRKRAAQFLEDLADLVENRAPKALEARIEQDEAAAERFDFTRGDYRI
                     LIALREDYLAHLEGVKGIMPSITQNRMRLARMTGAQALTAVLKPGGRLVSEEVAESIV
                     RFIAGGSELPNAEVEPSLLSLICRELNNARIAQGRTEISADLLAGSRDTILSEFYERA
                     LADQPPGVRQFIEDEMLTESGFRESLAEERVRKAFTAVGAPPDTLAKLVDRRLLRIEE
                     RLDMRRVELTHDVLCSVVKASRDLRYEREARDEAERQLVAQRARELATRKALVRARQV
                     AVICGILAIGALGSALFGYLSMKRAQDAELKAQQTRQMAETARGEAEKLIVYLLDDFY
                     LELEPVGRLDIVAALSKRAIDYYAALPVELRTPETDRNRALALVRYGAALRYQSKLEE
                     GQKVLAEAIDTLDALRKQGDTSE"
BASE COUNT          335 a          625 c          634 g          306 t
ORIGIN      
        1 cggcgaagtg acgcccgagt tcgcccgccg cctgcaggat ctgctttcaa cccggcactg
       61 aacggtcgcg tgcacagctc gcgtccgcat caggtattga tcgccatgcg attggcccga
      121 aaccctttat tcgatgagta acgacgcgtc tccggcgacg gacaacgcgc tctcgcaggc
      181 ggccgtcgac cacgagcatc catggctcgg cctggcctcg ttcaccgagg agacgcgcgc
      241 ctatttctat ggccgcgagg aggagatcgc cgagctttcg cggcgcgtgc agcgcaagct
      301 gctgacgatc ctgttcggtc aatcgggcct cggcaagacg tcgatcctgc gcgcggggat
      361 cgtgccgcgg cttcgtcccg agggctactg cccggtctac gtccgaatcg actattcgcg
      421 cgacgccccc gagccgtcgg agcagatcaa ggaagccatt ttccgcgcga cgcagtcgct
      481 cggacagtgg acgcagacag gtgtcgccgt cagcggcgaa tcgctgtggg aattcctgca
      541 ccaccgcgac gatgtcctgc gcgactcgtc gggcaggacg ctgatcccgc tgctcatatt
      601 cgaccagttc gaggaaatct tcacgcttgc gcaaagcgac gacttcggcc gcaagcgcgc
      661 tgcgcaattt ctcgaagatc ttgccgatct ggtggagaac cgcgcgccga aagcgctcga
      721 ggccaggatc gagcaggatg aggctgccgc cgagcggttc gacttcaccc ggggcgatta
      781 ccggatcctg atcgcgctgc gcgaggacta tcttgcccat ctggaaggcg tgaagggcat
      841 catgccctcg atcacgcaga accgaatgcg gctcgcccgc atgaccggcg cgcaggccct
      901 caccgcggtg ctgaagcccg gcggcaggct ggtttcggag gaagtggcgg agtcgatcgt
      961 gcgtttcatc gccggcggct ccgagctgcc gaacgccgag gtcgagccgt cgctgttgag
     1021 cctgatctgc cgcgagctca acaacgcccg catcgcgcaa gggcgcaccg agatctctgc
     1081 cgatctgctg gcaggctctc gagacacaat cctgagcgag ttctatgagc gcgcgctggc
     1141 cgaccagccg cccggcgtaa ggcaattcat cgaggacgag atgctgaccg agtcgggctt
     1201 ccgcgagagt ctggccgaag agcgcgtgcg caaggcattc accgcggttg gtgcgccccc
     1261 cgatacgctg gcgaagctgg tcgaccgtcg gctgctgcgc atcgaggagc gcctcgacat
     1321 gcgccgggtc gagctgaccc atgacgtcct gtgcagcgtc gtcaaggcaa gccgcgattt
     1381 gcgctacgag cgcgaggcgc gtgacgaagc ggaacgccag ctcgtcgcac agcgcgcgcg
     1441 cgagcttgcc acgcgcaagg cactggtgcg ggcgaggcag gtcgccgtga tctgcggaat
     1501 acttgccatc ggcgccctgg gcagcgcgct gttcggctat ctgagcatga agcgcgcgca
     1561 ggacgcggag ctcaaggcgc agcagacgcg ccagatggcg gagaccgcac ggggcgaagc
     1621 ggaaaagctc atcgtctatc tgctcgacga tttctacctc gagctcgagc ccgtcgggcg
     1681 gctcgacatc gtcgccgcat tgtccaagcg cgcgatcgac tattacgcag cgctcccggt
     1741 ggagctgcgc acgcccgaga ccgaccgcaa ccgcgcgctc gcgctggtgc gctacggcgc
     1801 cgcgctccgt taccagtcga agctcgagga gggccagaag gtcctcgccg aggcgatcga
     1861 cacgctggac gctttgcgca agcagggcga cacgtcggag
//
LOCUS       DATANS010000096         1570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_173136, whole genome shotgun sequence.
ACCESSION   DATANS010000096 DATANS010000000
VERSION     DATANS010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173136"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VHT22_01420"
     CDS             <1..484
                     /locus_tag="VHT22_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretin"
                     /protein_id="HEX3136238.1"
                     /translation="SQIDTTHTVSVSNGVAVPALIVRRASTTVELRDGQSFVIGGLLQ
                     SDNKNAIEQLPWLGSIPVLGTLFSSKSYQKNETDLAIIVTPRLVRPARPGDEIKAPTD
                     DTLPPNDVDFFLMGKNEITREQARKLTPATARVAVAADKPFTGHMIDLPKGESNATIQ
                     "
     gene            468..839
                     /locus_tag="VHT22_01425"
     CDS             468..839
                     /locus_tag="VHT22_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136239.1"
                     /translation="MRPFSNKLRAFALGALLSVLGGCSPYGEYLDRRDTIALSGGNAV
                     ATDAVTHRVDPWPLESANRNIAFNGAKMQTAMERYRTGRVIPPRGVGTSSAYQDAQGA
                     MSQNNGAPVGPPVTAPAAPVK"
     gene            898..>1570
                     /locus_tag="VHT22_01430"
     CDS             898..>1570
                     /locus_tag="VHT22_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008384578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver protein"
                     /protein_id="HEX3136240.1"
                     /translation="MVVLTADAAFEESVRATFGASSAIELSLVSGRLAEHSDTLDVTG
                     ATVVVVDIDASRADEMNGLARLMARVGTWPPVVAVTQSFDEHVARTLLQMRTADFLVK
                     PVEPVDLVRACARVSKGSRGGAEPTEAKIFTFLPAVGGAGVTTLAVQSAMILLNSGGP
                     RVKPSTCLVDLDFQHGSVADYLDLEPRLNLAEIEPRPDRLDRQLLEVMLSYHASGLAV
                     VAAPNR"
BASE COUNT          288 a          519 c          499 g          264 t
ORIGIN      
        1 cagccagatc gacacgaccc acaccgtctc ggtcagcaac ggtgttgccg tgccggcgtt
       61 gatcgtgcgt cgcgcctcga ccaccgtgga actgcgcgac ggtcagagct tcgtcatcgg
      121 cggcctgctg cagagcgaca acaagaacgc aatcgagcag ctgccgtggc tcggcagcat
      181 cccggttctg ggcacgctgt tctccagcaa gtcctatcag aagaacgaaa ccgaccttgc
      241 catcatcgtc acgccgcgcc tcgtgcgtcc ggcgcggccc ggggacgaaa tcaaggcgcc
      301 gacggacgac acattgccgc cgaacgacgt cgacttcttc ctgatgggca agaacgagat
      361 cacgcgtgaa caggcgcgca agctgacgcc cgccacagcc cgcgtggccg tcgccgcgga
      421 caagccgttc accggacaca tgatcgacct gccgaagggg gaatccaatg cgaccattca
      481 gtaacaaatt gcgcgcattc gccctcggcg cgctgctctc ggtgctcggc ggttgctcgc
      541 cttacgggga gtatctcgac cgccgcgaca ccattgcgct ctccggaggc aatgcggtgg
      601 cgacggatgc cgtgacacat agggtcgatc cgtggccgct tgagagcgca aaccgaaaca
      661 tcgctttcaa cggcgcgaag atgcagaccg cgatggagcg atatcggacc ggccgggtca
      721 ttccgccgcg cggcgtcggt acatccagcg cctaccagga tgcgcagggt gcgatgtcac
      781 agaacaacgg cgccccggtt ggcccgcctg tgaccgcccc ggccgccccg gtgaaataac
      841 ggaaagtgca gttgcatcat gcgcattggt cgccctccgg aaacaaatcg gacccgcgtg
      901 gtggtcttga ccgccgacgc tgcgttcgaa gaatcggtcc gcgcgacctt tggcgcatcg
      961 agcgcgatcg aactgtcact ggtctccggt cggctcgccg agcatagcga tacgctcgac
     1021 gttaccggcg cgaccgtggt ggtcgttgat atcgatgcga gccgcgccga tgagatgaac
     1081 gggctcgccc ggctgatggc acgcgtcggc acatggcctc cggtcgtcgc ggtgacgcag
     1141 agtttcgacg aacacgtcgc gcgcacgctg ttgcagatgc gcacggccga ctttctggta
     1201 aagccggtcg agccggtcga tctggtgcgg gcctgcgcac gcgtctcaaa aggatcgcgc
     1261 ggaggagccg agcccaccga agcgaagatc tttacttttc tgcccgcagt cggcggcgcc
     1321 ggtgtcacga ccctcgccgt gcagagcgcg atgatccttc tcaacagcgg cgggccgcgc
     1381 gtaaagccgt cgacgtgtct ggtcgatctc gattttcagc acggctcggt cgccgactat
     1441 ctcgacctcg agccgcgcct caatctggcg gaaatagagc cgcggcccga tcgtctggac
     1501 aggcagctgc tcgaagtcat gctctcgtat catgcgtcag ggctcgcggt cgtggcggcg
     1561 cccaatcgcc
//
LOCUS       DATANS010000097         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_130543, whole genome shotgun sequence.
ACCESSION   DATANS010000097 DATANS010000000
VERSION     DATANS010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130543"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VHT22_01435"
     CDS             <1..575
                     /locus_tag="VHT22_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX3136241.1"
                     /translation="HEFNASTFTARVIAGTGADMYSAIAGAIGALRGPKHGGANEVAF
                     EIQQRYETPAQAEEDIRARVANKEVIIGFGHPVYTISDPRNEVIKTVARKLAESSGDT
                     KMYSIAERLEAVMKQAKNMFPNLDWYSAVSYHLMGVPTAMFTPLFVVARTTGWAAHVI
                     EQRIDGKIIRPSANYTGPENLKFVPLDERK"
     gene            599..2050
                     /locus_tag="VHT22_01440"
     CDS             599..2050
                     /locus_tag="VHT22_01440"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HEX3136242.1"
                     /translation="MSTHDYKSAERPAPDKVLTDIADYALSYRIDSKAAYETAYYCLM
                     DTLACGFQALQYPACTKLMGPVVPGATLPGGARVPGTSYELDPVQAAFNIGTMIRWLD
                     FNDTWLAAEWGHPSDNLGAILAVADYLSRRNASQGAKGLVVRDVLTAMIKAHEIQGVT
                     ALENSFNRVGLDHVLLVRIASTAVAAAMLGGTRDQVINAVSNAWIDGGALRTYRHAPN
                     TGSRKSWAAGDATSRAVRHALISLTGEMGYPSALSAKTWGFQEVLFKGKALSYPQGLG
                     SYVMENVLFKISYPAEFHAQTAVEAAMTLHPQVANRLDQIERIVIETQEPGVRIIDKT
                     GKLANPADRDHCIQYMVAIPLIFGRLTAADYEDNIANDARVDALRSVMQVRENTQFTQ
                     EYYAADKRYIGNAVQVFFRDGSSTARVAVDFPIGHRKRRAEGMPVLVKKFEASVAQHF
                     TPKQTESIKALFADRARLEQMPVQEFVAGLVKN"
BASE COUNT          420 a          679 c          661 g          353 t
ORIGIN      
        1 agcatgaatt caacgcctcg accttcaccg cacgtgtcat tgccggcact ggcgccgaca
       61 tgtactcggc gatcgccggc gcgatcggcg cactgcgcgg tccgaagcac ggcggcgcga
      121 acgaagtggc cttcgagatc cagcagcgct acgagacacc cgctcaggcc gaggaagaca
      181 ttcgcgcgcg tgtcgcgaac aaggaagtga ttatcggctt cggtcatccg gtctacacga
      241 tctccgatcc gcgcaacgag gtcatcaaga ccgtcgcgcg caagctcgcc gagagcagcg
      301 gcgacaccaa gatgtattcg atcgccgagc gcctcgaagc ggtcatgaag caggcgaaga
      361 atatgtttcc gaacctcgac tggtattcgg ccgtgtccta tcacctgatg ggcgtgccga
      421 ccgcgatgtt cacgccgctg ttcgtcgtcg cgcgcaccac cggctgggcc gcgcatgtca
      481 tcgagcagcg catcgacggc aagatcattc gtccgagcgc caactacacc gggccggaga
      541 acctgaagtt cgtgccgctc gacgaaagaa aatgaacgag aggaactgac gagcacctat
      601 gtcgacccac gattacaaat ccgccgaacg acccgccccc gacaaagttc tcaccgacat
      661 cgccgactac gcgctgtcct atcgcatcga cagcaaagca gcgtacgaga ccgcgtacta
      721 ctgcctgatg gataccctcg cctgcggatt ccaggcgctg cagtacccgg cgtgcacgaa
      781 gctgatggga ccggtcgttc caggcgcaac gcttccgggc ggcgcgcgcg tgccgggcac
      841 ttcgtacgag ctcgatccgg tgcaggcggc gttcaacatc ggcacgatga tccgctggct
      901 cgatttcaac gacacgtggc tcgccgcgga gtggggccat ccgtcagata acctcggcgc
      961 gatcctcgct gttgccgatt acctctcgcg gcgtaacgcg tcgcagggcg ccaagggcct
     1021 cgtcgttcgc gacgtgctga cggcgatgat caaagcacac gagatccagg gtgtcaccgc
     1081 gctggagaac agcttcaatc gcgtaggtct cgaccacgtg ctgctggtcc gcatcgcttc
     1141 gacggccgtc gctgccgcga tgctcggcgg cacgcgcgat caagtcatca atgccgtgtc
     1201 gaatgcctgg atcgatggcg gtgcgctgcg cacctatcgt catgcgccga acactggctc
     1261 gcgcaagagc tgggccgcgg gtgatgccac gagccgcgcg gtacgtcacg cgttgatctc
     1321 gctcaccggc gagatgggct atccgtcggc gctcagcgcg aagacctggg gctttcaaga
     1381 agtgctgttc aagggcaagg cgctttcgta tccgcagggt ctcggcagct acgtgatgga
     1441 gaacgtgctg ttcaagatct cgtacccggc cgaatttcat gcgcagacgg cggtcgaagc
     1501 cgcgatgacg ctgcatccgc aggtcgcgaa tcgactcgac cagatcgagc gcatcgtcat
     1561 cgagacgcag gagccgggcg tgcgcatcat cgacaagacc ggcaagctcg ccaacccggc
     1621 ggatcgcgac cactgcatcc agtacatggt ggcgatcccc ctgatcttcg gtcgcctcac
     1681 cgccgcggac tacgaggaca acatcgcgaa cgatgcacgc gtcgacgccc tgcgcagcgt
     1741 catgcaggtg cgcgagaaca cgcagttcac ccaggaatac tacgcagccg acaagcggta
     1801 catcggcaat gccgtgcagg tcttcttccg cgacggcagc tcgactgccc gcgtcgcggt
     1861 ggatttcccg atcggtcatc gcaagcgccg tgccgaaggc atgcccgtgc tggtgaagaa
     1921 attcgaggca tcggtcgcgc agcacttcac gccgaagcag acggagtcga tcaaggcgct
     1981 gttcgcagac cgcgcgcggc tcgagcagat gccggtacag gagttcgttg ccgggctggt
     2041 aaagaactaa gaagaaggct ggcagctcgc cgcgagcagc cggtccgggg gttgttgggg
     2101 atgtccgcca cgt
//
LOCUS       DATANS010000098         2112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_509440, whole genome shotgun sequence.
ACCESSION   DATANS010000098 DATANS010000000
VERSION     DATANS010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509440"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VHT22_01445"
     CDS             <1..517
                     /locus_tag="VHT22_01445"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3136243.1"
                     /translation="NDRYQLSILNIPVDDTNTMFYFIAWSESKGIEQQAWREFCKARV
                     GVDLDSRFQRIRTLENNYLQDRDAMKHGSHTGIAGIPNQDIAMWETMGPIADRSLERL
                     GASDMAIVQFRRIMVDAARRFRDGGPAIGTSPQHVAHVKLKSFEGIVPKKTDWMTLGV
                     AAEELQASRTS"
     gene            677..1642
                     /locus_tag="VHT22_01450"
     CDS             677..1642
                     /locus_tag="VHT22_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3136244.1"
                     /translation="MSKRGRFCTAIAAVLIAMTAPAAQAEDTLRVAAGQRGNWDTTIA
                     EIGQRGGIFKKNGLSLEILYTQGGGETQQAVISGSVEIGVAPGIMGVLSAFSKGAPVR
                     IIGAETTGAADLFWYVPSNSPIKSLKDTDGKTIAYSTNGSSTHGIVTAFMKQYDLKAR
                     PTATGGPAPTLTQVMSNQIDVGWSAPPFGLQQLDEGKIRIIAVGNDATVFKGQTVRLL
                     ITNVQTLQERKPVIERFMKAYRETIDWLYSDDPAALKTYADFVGISVATAKRTRDGFF
                     PKAAVDPDAVVGLDQITQDAVTLKYTAAPLTKEQLAELIQIPPRR"
     gene            1666..>2112
                     /locus_tag="VHT22_01455"
     CDS             1666..>2112
                     /locus_tag="VHT22_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3136245.1"
                     /translation="MTTAPTRLLDVEHISKRYTGARDVITAVDDISFGVDPGEFVSII
                     GPSGCGKSTLFNIIGGLIGDYDGRVAVAGATIAGTHPAIGMVFQEESTFPWRTVADNV
                     AFPLELAGIGRRERLERAESFVTLVGLHGFGHRYPAELSGGMRQRVA"
BASE COUNT          420 a          686 c          669 g          337 t
ORIGIN      
        1 caacgatcgc taccagctgt cgatcctcaa cattcccgtc gatgacacga acacgatgtt
       61 ctatttcatc gcgtggagcg aatcgaaagg catcgagcaa caggcgtggc gcgaattctg
      121 caaggcccgg gtcggggtcg acctcgactc aaggttccaa cggattcgca cgctcgagaa
      181 caactatctg caggatcgcg acgcgatgaa gcacggcagc cataccggca tcgcgggcat
      241 tcccaaccag gacatcgcga tgtgggagac gatgggaccg atcgcggatc gctccctgga
      301 acggctgggc gcgagcgaca tggcgatcgt gcaattccgc cgcatcatgg tcgatgcggc
      361 gcgccggttt cgcgacggcg gcccagcaat cggcacttcg ccgcagcatg tcgcccacgt
      421 caagctcaag tccttcgagg gtatcgttcc caaaaaaacc gactggatga cgctcggcgt
      481 cgcggcggag gagcttcagg catcgcgaac gtcgtagtgg cgcgcccagg cgggaacgcg
      541 atacgcgcta tagcacgtca accgattgac gccgggcgaa ccgcacgcga cactgttgca
      601 cacgccaata gcagccgccg ccgcggaaaa caagcgcgcg agcgcactcg cccaggacag
      661 gaccaggagg aaaacaatga gcaaacgggg aaggttttgc actgcgatcg ccgctgtgct
      721 gattgcaatg actgccccgg ccgcgcaggc agaggacacg ctgcgggttg ctgccggcca
      781 gcgcggcaac tgggacacga cgatcgccga gatcggccag cgcggcggca tattcaagaa
      841 gaacggcctg agcctggaga ttctgtacac ccaaggggga ggcgaaaccc agcaggcggt
      901 gatctcgggc agtgtcgaga tcggcgtcgc accgggcatc atgggcgtgc tctccgcctt
      961 ctccaagggc gcgccggtgc gcatcatcgg cgccgaaacg accggcgcgg ccgacctttt
     1021 ctggtatgtg ccgtcgaatt cgccgatcaa gtcgctgaag gataccgacg gcaagacaat
     1081 cgcgtactcg accaacggct cgtcgacaca cggcatcgtc accgcgttca tgaagcaata
     1141 cgacctgaaa gccagaccga cggcgaccgg cggaccggcg ccgacgctca cccaggtaat
     1201 gtcgaaccag atcgacgtcg gctggtcggc gccgccgttc gggctgcagc agctcgacga
     1261 aggcaagatc cgcatcatcg cggtcggcaa cgatgcgacc gtattcaagg gccagaccgt
     1321 tcgcctgctg atcaccaatg tgcagacctt gcaggagcgc aagccggtca tcgagcggtt
     1381 catgaaggct taccgcgaga cgatcgactg gctgtattcg gacgaccccg ccgcgttgaa
     1441 gacctacgcc gatttcgtcg gcatttcggt cgcgaccgcc aagcgaacgc gtgacggctt
     1501 cttccccaag gccgcggtcg acccggacgc ggtcgtcgga ctcgaccaga tcacgcagga
     1561 tgccgttacg ctgaagtaca cggccgcgcc attgaccaag gagcagctcg ccgagctgat
     1621 ccagattcct ccccgccgct aggcttcagg tccggcgagt ccgtcgtgac cacggcgcct
     1681 acccggctgc tcgatgtcga gcacatcagc aagcgttaca ccggcgctcg agacgtcatc
     1741 accgcggtcg acgacatctc gttcggcgtc gatccgggcg agttcgtctc gatcatcggg
     1801 ccttcggggt gtggcaagtc gaccttgttc aacatcatcg gcggcctgat cggcgactac
     1861 gacggccgag tcgctgtcgc cggcgcgact atagccggca cgcatccggc gatcggcatg
     1921 gtgttccagg aagagtcgac gttcccctgg cgcaccgtcg ccgacaacgt tgcgtttccc
     1981 ctcgagctcg caggcatcgg acggcgcgag cgcctcgagc gcgcggagtc gttcgtgacg
     2041 ctggtcggcc tgcatggatt cggccaccgc tatcctgccg agctgtcggg cggcatgcgg
     2101 caacgcgtcg cc
//
LOCUS       DATANS010000099         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_635265, whole genome shotgun sequence.
ACCESSION   DATANS010000099 DATANS010000000
VERSION     DATANS010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635265"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..127
                     /locus_tag="VHT22_01460"
     CDS             <1..127
                     /locus_tag="VHT22_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetic acid degradation protein"
                     /protein_id="HEX3136246.1"
                     /translation="GEVDMDVNFALEDYEVARGFILCCQSYPVTDKVVVDFDQGA"
     gene            194..>1783
                     /gene="paaN"
                     /locus_tag="VHT22_01465"
     CDS             194..>1783
                     /gene="paaN"
                     /locus_tag="VHT22_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaN"
                     /protein_id="HEX3136247.1"
                     /translation="MSHPLFERHRALLDQALKAIAERGYWSAFPESPSPKVYGEGSAD
                     AGKAAFDALANKPFPIDLPGTVGTVGGERSPYGIALGATYPRVDLDQLFAAIAVAEKS
                     WRRAGPEAWVGVCLEILSRINKRSFEIAHAIMHTTGQAFVMSFQAGGPHAQDRGLEAV
                     AYAWDEMRRIPASAYWEKPQGKNEPLKMEKRYAIVPRGIALVIGCNTFPTWNGYPGMF
                     ASLATGNAVIVKPHPNAVLPLAITVAIAREVLAEAGFDPNVVTLVAHRVDDDTAQALA
                     LRPEVKLIDFTGSSAHGTWLESNAQQAQVYAEKAGVNQIIIDSADDFAGAARNIAFSL
                     ALYTGQMCTAPQNIYVPRDGIETGDGHLSFDHVAQGLGAAVQKLVGDPTRAVEILGAV
                     VNDGVVERIHAARSLGAVVHDSQSLVHPQFPDARIVTPLLVKLDATDGAKYLKEWFGP
                     IAFVIATDDTAQSLGIARRAVVDHGALTLSLYSNRPDVIENTIALAEDAGVALSINLT
                     GGVFVNQSAAFSDYHGTGANPA"
BASE COUNT          315 a          575 c          587 g          306 t
ORIGIN      
        1 gggcgaagtc gacatggatg tcaatttcgc gctcgaggac tacgaggtcg cgcgcgggtt
       61 cattctgtgc tgccagagtt atccggtcac cgacaaggtc gtcgtcgact tcgatcaggg
      121 cgcatgagct tgccgcgggc gccgcagcta cgcaaccgtt gctatcgttg aaccagagtc
      181 aggagagcac gccgtgtccc atccgttatt cgagcgccac cgcgcgttgc tggaccaggc
      241 gctgaaggcg atcgccgagc gcggctactg gtccgcgttc cccgagtcgc cgagcccgaa
      301 ggtttacggc gaaggttccg ccgacgccgg caaggcggcg ttcgacgcgc tggccaacaa
      361 gccgtttccg atcgatctgc ccggaacggt cggcaccgtg ggcggcgagc gctcgccgta
      421 tgggatcgcg ctcggcgcta cctatccacg cgtcgacctc gaccaactct ttgccgcgat
      481 cgccgtcgcg gaaaagtcgt ggcgcagggc cggtcccgaa gcctgggtcg gcgtctgcct
      541 cgagattctc tcgcgtatca acaagcgcag cttcgagatc gcacacgcca tcatgcatac
      601 gaccggacag gcgttcgtga tgtcgttcca ggccggcggc ccgcatgcgc aggaccgtgg
      661 gctggaggct gtcgcctacg cgtgggacga gatgcggcgt atcccggcca gcgcctactg
      721 ggaaaagccg caaggtaaga acgagccgct gaaaatggaa aagcggtatg cgatcgtacc
      781 gcgcggcatc gcattggtca tcggttgcaa cacgttcccg acctggaacg gctacccggg
      841 catgttcgcg agcctggcga ccggcaacgc ggtcatcgtc aagccgcatc cgaacgctgt
      901 cctgcccctc gcgatcaccg tagcgatcgc acgcgaagtg ctggcggagg cgggcttcga
      961 tccgaacgtc gtgacgctgg tcgcgcaccg ggtggacgac gacaccgcgc aagcgcttgc
     1021 gttgcgtccc gaggtcaaat tgatcgattt caccggaagc agcgcgcacg gaacgtggct
     1081 cgagagcaac gcgcagcagg cgcaggtgta cgccgaaaag gcgggcgtca accagatcat
     1141 catcgactcg gccgatgatt ttgccggcgc ggcgcgcaac atcgcttttt cgctggcgct
     1201 atacaccggc cagatgtgca cggcgccgca gaatatctac gttccgcgcg acggcatcga
     1261 gaccggcgac gggcacctgt cgttcgacca cgtcgcgcaa ggtctgggcg ctgcggtgca
     1321 gaagctggtc ggcgatccga cgcgcgcggt ggagattctg ggggcagtgg tcaacgacgg
     1381 cgtcgtcgag cggatccacg ccgcgcgctc gctgggtgcg gtcgtgcacg acagccagtc
     1441 gctggttcat ccgcagttcc ccgacgctcg catcgtgacg ccgctgctgg tcaagctcga
     1501 cgccaccgac ggagccaaat atctgaagga atggttcggt ccgatcgcgt tcgtcatcgc
     1561 caccgacgac accgcgcaga gtctaggcat tgcgcgtcgc gcggtcgtcg accatggtgc
     1621 gctgaccttg tcgctctact cgaaccgccc ggatgtgatc gagaacacga tcgcgctggc
     1681 cgaagacgcc ggcgtcgcgt tgtcgatcaa cctgaccggt ggagtgttcg tcaatcagtc
     1741 ggcggctttc tccgactatc acggcaccgg cgccaatccg gcc
//
LOCUS       DATANS010000100         1838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_340378, whole genome shotgun sequence.
ACCESSION   DATANS010000100 DATANS010000000
VERSION     DATANS010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340378"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VHT22_01470"
     CDS             complement(<1..1100)
                     /locus_tag="VHT22_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HEX3136248.1"
                     /translation="MASFSEIVEQARLSARALLDYGDNLFNPTVRLGVTGLSRAGKTV
                     FITALVHGLSRGGRFPVFEPLATGRIARARLEPQPDDAVPRFAYEDHLRTLIEQRRWP
                     NSTVDISELRLVIDYQRQNGADRTLTLDIVDYPGEWLLDLPLLTKNYEQWAAESLALS
                     RQEPRARLAAEWHAHLATLDAQAHENEQATLTAARLFTAYLRVCRDERFAMSLLPPGR
                     FLMPGSLAGSPALTFAPLDVPVGGSAPEGSLWAMMRRRYEAYKDVVVRPFFRDHFSRL
                     DRQIVLVDALAAFNAGPEALHDLEAALAGILDCFRVGRSTIFSTLFRPRIDRILFAAT
                     KADHLHHSSHDRLEAILRRMLDRTAMRAELAGA"
     gene            1279..>1838
                     /locus_tag="VHT22_01475"
     CDS             1279..>1838
                     /locus_tag="VHT22_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX3136249.1"
                     /translation="MTVFRLKQLASTSANAAMGAVRWNYDRAELIADGVVHGIGVLGG
                     LVAATVLIVLTALYATALEVAVVSIYLAGLLTMLVLSATYNLWPVSRAKWVLRRFDHS
                     AIYVLIAATYTPFIMEIKDSILAVALLVGVWCLAIVGIVLKLSLPGRFDRLSVGLYLA
                     MGWSGFMLYDAVVAALPAMALWFVITG"
BASE COUNT          323 a          573 c          603 g          339 t
ORIGIN      
        1 gcgccggcga gctcggcgcg catcgccgta cgatccagca tgcggcgcag gatggcttcc
       61 agccggtcgt gactggaatg gtgcaggtga tcggccttgg tggcggcaaa caggatgcgg
      121 tcgatccgcg gccggaacaa cgtactgaaa atcgtgctgc ggccgacccg gaaacaatcg
      181 agaatgcctg ccagcgccgc ctcgagatcg tgcagcgctt ccggcccggc gttgaacgcc
      241 gccagcgcat cgaccagcac gatctgccgg tccagccgcg aaaaatgatc gcggaaaaac
      301 gggcgcacca ccacgtcctt gtaagcttcg taacgccgcc gcatcatcgc ccacagcgat
      361 ccctcgggcg ccgatccacc gaccggcacg tcgagcggcg caaaggtcag cgccggcgat
      421 cccgcgagac tgcccggcat caggaagcgc cccggcggca gcaggctcat ggcgaagcgt
      481 tcgtcgcggc agacgcgcag gtatgcggtg aacagtcttg ccgcggtcag cgtcgcctgt
      541 tcgttctcgt gcgcttgcgc gtcgagcgtc gcaagatgcg catgccattc cgccgcgagc
      601 cgcgcgcggg gctcttgccg cgacagcgcg agactttccg ccgcccactg ttcgtaattc
      661 ttggtgagca gcggcagatc gagcagccat tcgccgggat agtcgacgat atcgagggta
      721 agcgtgcggt ccgcgccatt ttgccgctga tagtcgatca ccagccgcaa ttcgctgatg
      781 tcgaccgtcg aattcggcca gcggcgctgc tcgatcaagg tgcgcaggtg gtcttcgtaa
      841 gcaaaccgcg gcactgcatc gtcaggctgc ggctccagcc gcgcgcgggc gatccgccct
      901 gtcgccaggg gctcgaacac cggaaagcgg ccgccgcgcg agagcccgtg aaccagcgcg
      961 gtgatgaaca cggtttttcc cgcgcgtgac aatccggtga caccgagccg caccgtcgga
     1021 ttgaacaggt tgtcgccgta gtccagcagg gcccgcgcgg aaaggcgcgc ttgctcgacg
     1081 atttcggaga aactggccat gctgggatga gcggctcgac aatgagggaa tgcagacgct
     1141 gggaagctct tcagaaaagt ggcgactatc gcgccaaaat caagtttcat gatcagagga
     1201 aactttgcgg ttctgctttc gttgacctgc ccaagtgtat gatctatcgc tgggccagga
     1261 agctcgccgg accgatccat gaccgtcttc agactgaagc aactcgcctc gacgtccgca
     1321 aatgcggcga tgggggccgt gcgctggaat tacgaccgcg ccgaactgat cgcggacggc
     1381 gtcgtgcatg gcatcggcgt gctcggcgga ttggtcgcgg cgaccgtgct gatcgtgctg
     1441 acggccctct atgccacggc gttggaagtg gccgtggttt cgatttacct cgccggcctg
     1501 ctgaccatgc tggtgctgtc ggcaacctac aatctgtggc cggtatcgcg agccaaatgg
     1561 gtgctgcgcc gcttcgatca ttcggcgatc tatgtgctga ttgcggcgac ctacacgccg
     1621 ttcatcatgg agatcaagga cagcatcctc gccgtcgcgc tgctggtagg cgtatggtgc
     1681 ttggccatcg tcggcatcgt gctcaagctg tcgttacccg gccggttcga ccggctgtcg
     1741 gtcggcctct atctcgcgat gggctggagc gggttcatgc tgtacgatgc ggtggtcgca
     1801 gcgctgcccg ccatggcgtt gtggttcgtc attacagg
//
LOCUS       DATANS010000101         2195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_214857, whole genome shotgun sequence.
ACCESSION   DATANS010000101 DATANS010000000
VERSION     DATANS010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214857"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..>2195
                     /locus_tag="VHT22_01480"
     CDS             44..>2195
                     /locus_tag="VHT22_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX3136250.1"
                     /translation="MRDAVQGIEDVQTHSAQRSFWWTVGALLESIVERGVESGFGVKQ
                     LCGRIDLQIRRFVEGSAKVSDRMRREVLYYVAISAPVGAQTQAVQRAYHLNGLIPTAE
                     VLSADVLRTQPLLREAREQLASAKDLWLKITGGRPENIAKLKQTMAGLVDRVRAIGEP
                     SLSRLIGALASRVDKFGTGAVSEAVAMEFATALLLAESTIEHFGSISPELPQQIDAML
                     ARIDAAQQNKPTPLIAGAPLLDEMARRAQERLLLAQVAREIQANLRHMEQVLDAFFRD
                     NSKRAELATLAGDSRQITGALKMLGLDSAERLLELCQQQIDEYAKPDVEVHNDDLEML
                     AESLSGLGFYIEAVEQQRPDRDRLITPLLDRRLGVAPSAPAAEPETVESAVVDLRSAL
                     PEIFAAYQSAPGDLAARDRLSADLTTLKHDAELIGDARLQQDADAALTELAHAGTADT
                     AALQDAVAAVAAQSEAAPAPSAETMRLLETDATQFDTELLDIYLTEAGEVLDTIAASA
                     TELAANSDDRTALSVVRRGFHTLKGSGRMVGLTDLGELAYAVERVHNRVIEEDRPTTP
                     ALIALIGVAESSFRRWVDALRSTGRVTPDADALRAALAKVESEFPDGGGDSGGQPPSP
                     PPSPSRPTPGPLAAVSAIDRGPDIAVPGAWHAAIEVLELDETPYGEGDVVDSPPESAA
                     PTLAFAAVAAQDAPDTDAVATLADAEDEITVGGVT"
BASE COUNT          386 a          726 c          748 g          335 t
ORIGIN      
        1 cctgcttgct tggctgcgcg gcgacgagga agggtcgaag atcatgcgcg acgccgttca
       61 gggcatcgaa gacgtacaga cgcattcggc gcagcgctcg ttctggtgga cggtcggcgc
      121 actgctcgaa agcatcgtcg aaagaggcgt cgaaagcgga ttcggcgtca agcagctgtg
      181 tggacggatc gacctgcaga tccggcgctt cgtcgaaggc tcggcgaagg tctcggaccg
      241 gatgcgccgc gaggtgctct attacgtcgc gataagcgcc ccggtcggcg cacagacgca
      301 ggccgtccag cgcgcctatc acctcaatgg gctgataccg accgccgaag tgctgtcggc
      361 cgacgtgctg cgcacgcagc cgctgctgcg cgaggcccgc gaacagctgg ccagcgccaa
      421 ggacctctgg ctgaagatca ccggcgggcg tccggaaaac atcgccaagc tcaagcagac
      481 gatggcaggt cttgtcgacc gcgtgcgagc catcggggag ccctcgctgt cccgcctcat
      541 cggcgcgctg gccagccgcg tcgacaagtt cggcacgggt gccgtttccg aggccgtggc
      601 gatggaattc gcaacggcct tgctgctcgc cgaatcgacg atcgagcatt tcggctcgat
      661 ctcgccggag ctgccgcagc agatcgatgc aatgctggcg cgcatcgacg ccgcgcagca
      721 gaacaagccg acgccgctga tcgcgggcgc gccgctgctc gacgaaatgg cgcgtcgcgc
      781 gcaggagaga ttactgctcg cgcaagtcgc gcgcgaaatt caggcgaacc tccgccacat
      841 ggaacaggtg ctcgacgcgt ttttccggga caacagcaag cgcgccgagc tagccacgct
      901 cgctggcgac agtcgacaga tcaccggcgc gctgaagatg ctggggctcg acagcgcgga
      961 gcgtctgctt gagctctgcc agcagcagat cgacgagtat gcgaaacccg acgtcgaggt
     1021 tcacaacgac gatctcgaga tgctggccga atcgctgtcg ggtctcggct tctatatcga
     1081 ggccgtcgag cagcagcggc cggatcgcga ccgcctgatc accccgctgc tcgatcgccg
     1141 ccttggcgtt gcgcccagcg cgcccgccgc cgagcccgag accgtcgagt cggctgtcgt
     1201 cgatctgcga tcggcgctgc cggaaatctt tgccgcttat cagagcgcgc ctggcgacct
     1261 ggcggcgcgg gacaggctat cggccgatct gacgacgctg aagcacgatg ccgagctgat
     1321 cggcgacgcg cgcctgcagc aggacgccga tgcggcgctg accgagctcg cgcatgccgg
     1381 cacagcagac actgcggccc tgcaggacgc cgtcgcggcg gttgccgcgc aaagcgaggc
     1441 cgcgcccgct ccttccgcgg aaacgatgcg cctgctggaa acggacgcga cgcagttcga
     1501 taccgagttg ctcgacatct atctgaccga agcaggcgaa gtgctggata cgatcgcggc
     1561 gagcgcgaca gagctcgccg cgaattccga cgatcgcacg gcattgtcag tcgtgcgtcg
     1621 cggcttccac accttgaagg gcagcggccg catggtcggg ctcaccgatc tgggcgagct
     1681 cgcgtacgca gtagaaagag ttcacaaccg cgtgatcgag gaagatcgtc cgacgacgcc
     1741 ggcgctgatc gcattgatag gtgtggccga gtcaagcttt cgccgctggg tcgatgcgct
     1801 gcgcagtact ggccgggtga cgcccgatgc cgacgccttg cgcgcagcgc tggccaaggt
     1861 cgaaagcgag tttcccgatg gcggaggcga ctcgggaggg cagccgccat cgccgccgcc
     1921 atcgccatcg cggccgacgc cggggccgct cgccgccgtt tcggcgatcg accgcggccc
     1981 cgatatcgcc gtgccgggcg cctggcatgc ggcgatcgaa gtgctcgagc tcgacgagac
     2041 tccgtacggc gaaggcgatg tggtggattc tccgcccgag tcggcggcgc cgacgctggc
     2101 ctttgccgct gtcgcggcac aggacgcacc cgacaccgat gccgtcgcaa cgcttgccga
     2161 tgccgaagac gaaattaccg tcggcggagt cacgc
//
LOCUS       DATANS010000102         2840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_257144, whole genome shotgun sequence.
ACCESSION   DATANS010000102 DATANS010000000
VERSION     DATANS010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257144"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VHT22_01485"
     CDS             complement(<1..871)
                     /locus_tag="VHT22_01485"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3136251.1"
                     /translation="MPNEPHSWRHRLPVALMLLWLAGIGLRLTILAVPPVLAMIRDDF
                     ALSATEVGLLSSIPPALFAIAALAGSLLVARLGVTTALVGGLLLVAIGSALRGLATDF
                     AILFATTIVMSAGIAIMQPIMPAAVRLWTPSRIGLGTAIYTNGLLVGEIFPVLLTIPY
                     VLPWVGGDWRMSLVAWSAPIAVIAVVVYAGAPRSSALRDAPATMPRRWLPDWHRSLVW
                     RLGGLFCCINAIYFSANAFIPIYLISAGRPDLISGALTALNFGQLPASLLLLAFAERL
                     ERKAWPFLASGLLS"
     gene            complement(1027..1791)
                     /locus_tag="VHT22_01490"
     CDS             complement(1027..1791)
                     /locus_tag="VHT22_01490"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX3136252.1"
                     /translation="MLIKSWRTIAALALAAAFVAGAIPAHAEGITSEQAQQILDELKA
                     IRKAVEQGGGQGAGQPAPAPDDKVSFAFPAGGLSEGKANAPLVLVEYTDYQCPFCQQF
                     HNAAYPQIKTNYIDSGKVRFVSRDFPLDFHENARRSAYAARCAAEQGKFWELRHAMIV
                     NASQLQQDKINSYAANVKMDVAKFQSCIAADKYKAAIDKDIAEGMAAGVNGTPSFVLG
                     RVVDGKLVGVRMVGAMPYEQFDAKIQEMLAQAAAKN"
     gene            complement(1970..2425)
                     /locus_tag="VHT22_01495"
     CDS             complement(1970..2425)
                     /locus_tag="VHT22_01495"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX3136253.1"
                     /translation="MLSLRAGITLEQLAQRGKGFLPGLIGVRFTAVTEGEVDAELDIR
                     AELLAPNGYLHAATVIALADTACGYGCLAHLPAGADNFTTIELKSNFLGTARAGTLAC
                     VATPVHLGNATQVWDAVVRDRGSGKKIALFRCTQMVLWPRREVTAARQT"
     gene            complement(2457..>2840)
                     /locus_tag="VHT22_01500"
     CDS             complement(2457..>2840)
                     /locus_tag="VHT22_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX3136254.1"
                     /translation="LASSQGGDLSAQAVEVNGHGGPIRVLAATIEGADAKVLRETLDQ
                     LKGRFKSAAIVLGSTEGGKVTLIAGVTSDLTARIKAGDLVNHVATQVGGKGGGRPDMA
                     QAGGTDAAALPAALASVRGWVEQRL"
BASE COUNT          478 a          940 c          942 g          480 t
ORIGIN      
        1 gagagagcag gcccgatgcc agaaaaggcc acgccttgcg ctcgagtctc tcggcgaacg
       61 ccagcagcag caacgacgcc ggcagctggc cgaaattgag cgcggtgagc gcgccgctga
      121 tcaggtccgg ccggcccgca ctgatcaggt agatcgggat gaaggcgttg gcgctgaagt
      181 agatcgcgtt gatgcagcag aacaagcctc cgagacgcca gaccagcgat ctgtgccagt
      241 cgggaagcca ccgccgcggc atcgtcgcgg gcgcgtcgcg cagcgcgctg ctgcgcggcg
      301 cgccggcgta gacgacgacc gcgatcaccg cgatcggcgc cgaccacgcg accagcgaca
      361 tccgccagtc gccgccgacc cacggcagca catagggaat cgtcagcagc accggaaaga
      421 tctcgccgac gagaaggccg ttggtataga tcgccgtgcc gaggccgatg cgcgacggag
      481 tccatagtcg aacggccgcc ggcatgatcg gctgcatgat cgcaatgccg gccgacatga
      541 cgatcgtcgt tgcgaacagg atcgcgaaat cggtcgcgag tccgcgcaga gcggagccga
      601 tggcgaccag cagcaagccg ccgaccagcg cggtggtgac tcccagccgc gcgaccagca
      661 gcgatcccgc cagcgcagcg atggcgaaca acgcaggcgg aatgctggag aggaggccga
      721 cctcggtcgc gctcaaggcg aagtcgtcgc ggatcatcgc cagtaccggc ggcacggcga
      781 ggatcgtcaa cctcaaaccg atgccggcga gccagagcag catcagcgcc acgggaagcc
      841 tatggcgcca ggagtgtggt tcgttgggca tcgtgtcggt ggcgtcgccg cattatcgcc
      901 gaggcggggg tcagcggagt gcgcggcggc gtcgcctttc tgctcgcgat ggcgcaaaaa
      961 aagcttcggc cggcgcgggc tttcactgcc cgggccggcc gaagccggat gacgttattc
     1021 agtcgatcag ttcttggctg cggcctgggc cagcatttcc tggatcttgg cgtcgaactg
     1081 ctcgtacggc atcgcaccga ccatgcgcac gcccaccagc ttgccgtcca cgacgcggcc
     1141 gagcacgaac gacggcgttc cgttgacgcc ggccgccatg ccttcggcga tgtccttgtc
     1201 gattgccgcc ttgtacttgt ccgcggcgat gcacgactgg aacttggcga catccatttt
     1261 cacgttagcc gcatagctgt tgatcttgtc ctgctgcagc tggctcgcgt tgacgatcat
     1321 cgcatgccgc agttcccaga attttccctg ctcggccgcg catcgcgccg cgtaggcgct
     1381 gcgccgcgcg ttctcgtgga agtcgagagg gaaatcgcgg ctgacaaagc ggaccttgcc
     1441 gctgtcgatg taattggtct tgatctgcgg atacgccgcg ttgtggaact gctggcagaa
     1501 cggacactgg tagtcggtgt attcgaccag gaccagcggc gcgttggcct tgccctcgct
     1561 caagccgccg gccgggaagg cgaagctgac cttgtcgtcg ggcgccggcg cgggttggcc
     1621 tgcgccctga ccgccgccct gctcgaccgc cttgcggatc gccttgagct catcgaggat
     1681 ctgctgcgcc tgctcgctgg taatgccttc ggcgtgcgcc ggaatcgcgc ctgccacaaa
     1741 agcggcggcc aacgcgagcg cggcgatggt gcgccaggat ttgatcaaca ttgccttgcc
     1801 tgccttcctt tgagttatcg acgtccgggc cgggttcagg gaaactgagc ccgcctcaag
     1861 attggccgcc atcatagggc gcgagcccgg catcgtcgcg acggcccgat gtgaggcatt
     1921 tgacgaagaa tgaacggttg gcgacgatcg tgcccgctta cgcgcagcgt caggtttggc
     1981 gggccgccgt cacctctcta cgcggccata acaccatctg cgtgcagcgg aacagcgcta
     2041 ttttcttgcc ggagccgcga tcgcgcacga cggcatccca cacctgggtc gcattaccca
     2101 ggtgaaccgg cgtcgcgacg caggctagtg tgccggcgcg ggcggtcccg agaaaattgc
     2161 tcttgagctc gatggtcgta aagttgtcgg cgccggccgg cagatgcgcg aggcagccat
     2221 agccgcatgc ggtgtcagcc agcgcgatga ccgtcgctgc gtgcaggtag ccattcggcg
     2281 cgaggagctc ggcgcggatg tcgagctcgg cgtcgacttc tccttcagtc acggcggtga
     2341 accgcacgcc gatcagcccc ggcaggaagc ctttgccgcg ttgcgcgagc tgttcgagtg
     2401 tgatgccggc gcgcaagctc aacacgttcc cctttttgtt gcgatgaccg cgtagcctac
     2461 agccgctgct cgacccagcc ccgcacactg gcaagcgccg cgggcagtgc ggcggcgtcg
     2521 gtgcctcccg cctgtgccat gtcgggccgg ccgccgccct tcccgccgac ttgggtggcg
     2581 acgtggttga ccagatcacc cgccttgatt ctcgccgtca gatccgatgt aaccccggcg
     2641 atcagcgtca ccttgccgcc ctcggtcgag cccagcacga tggcggccga cttgaaccga
     2701 cctttcaact ggtcgagcgt ttcgcgcaga accttggcat cggcaccttc gatcgtcgcg
     2761 gcgagcacgc ggatcggtcc gccatggccg ttgacttcga ccgcctgcgc cgacaggtcg
     2821 ccgccctggc tcgacgcgag
//
LOCUS       DATANS010000103         1596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_214873, whole genome shotgun sequence.
ACCESSION   DATANS010000103 DATANS010000000
VERSION     DATANS010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214873"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1596
                     /locus_tag="VHT22_01505"
     CDS             <1..>1596
                     /locus_tag="VHT22_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020831969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX3136255.1"
                     /translation="HVTKLDIVNNRLIPNAIEPRAAVASYSRADEGYTLYVTSQNPHV
                     ERLLMTAFVLGLPETKVRVIAPDVGGGFGSKIYLYPEETALVWASKKVNRPIKWTAER
                     SESFLSDAHGRDHMTHAELALDKDGKFLGMRVQTTAAMGAYLSTFASCIPTILYATLL
                     AGQYTTPAIWCEVTAVFTNTAPVDAYRGAGRPEATYVVERLVHAAAVEMGIAQDELRR
                     RNFIRKFPHQTPVALLYDIGDYDACLDEAMKMADVKGFPARKAEAAKRGQLRGIGYAS
                     YIEACGIAPSNIAGALGARAGLFETGQVRVNPTGGVTVFTGSHSHGQGHETTFSQVVA
                     SRLGIPIENVEIVHGDTGRIPFGMGTYGSRSLSVGGTAIVKALDKVIAKGRKIAAHLL
                     EAAEADIEFGDGKFTVAGTDRSKTFGEIALAAYVPHNYPLDKLEPGLEETAFYDPTNF
                     TFPAGTHICEVEVDPETGVVQVVNFSACDDFGNIINPMIVEGQVHGGVAQGIGQALLE
                     CCVYDKDSGQLLTGSYMDYAMPRS"
BASE COUNT          301 a          511 c          517 g          267 t
ORIGIN      
        1 cacgtgacca agctggacat cgtcaacaat cggctgattc cgaacgcgat cgagccgcgc
       61 gcagcggttg cgagctacag tcgtgccgac gagggctata cgctctacgt gaccagccag
      121 aatccgcacg tcgagcgctt gttgatgacc gcgttcgtcc tgggcctccc ggagaccaag
      181 gtgcgcgtga tcgcgcccga tgtcggcggc ggtttcggct ccaagatcta cctgtatccg
      241 gaggagaccg cgctggtctg ggcatcgaaa aaggtcaacc gcccgattaa atggacggcc
      301 gagcgcagcg aatccttcct gtccgacgcg cacggccgcg accatatgac gcatgccgag
      361 ctggcgctcg acaaggacgg caagttcctg ggcatgcggg tgcagaccac tgcggcgatg
      421 ggggcgtacc tgtcgacgtt cgcctcgtgc atcccgacga ttctttacgc gacgctgctc
      481 gccggccagt acactacgcc ggcgatctgg tgcgaggtga ctgccgtgtt cacgaatacc
      541 gcgccggtcg acgcctatcg gggcgcagga cggccggagg cgacctacgt cgtcgagcgg
      601 ctggtccatg ccgcggcggt ggaaatgggc atcgcgcagg acgaactgcg ccgccgaaac
      661 ttcatccgca agtttcccca ccagacgccg gtggcgcttc tctacgatat cggcgattac
      721 gacgcctgcc tggacgaggc gatgaagatg gccgacgtca agggatttcc cgcgcgcaag
      781 gccgaagcgg ccaagcgcgg gcagttgcgc ggtatcggct acgcgtcgta catcgaggcc
      841 tgcggtatcg caccgtcgaa catcgcgggc gcgctcggcg cgcgcgccgg gctgttcgaa
      901 accgggcagg tgcgcgtcaa tcctaccggc ggcgtcaccg tgtttaccgg atcgcacagt
      961 cacggccagg gacatgaaac gacattctcg caggtggtcg cgagtcgcct tggaataccg
     1021 atagaaaatg tggagatcgt ccacggcgac accggccgca ttccgttcgg catgggaacc
     1081 tacggctcgc gctcgctctc ggtcggcggc acggcgatcg tcaaggcgct ggacaaggtg
     1141 atcgccaagg gccggaaaat cgccgcgcac ctgctcgagg ccgctgaagc cgacatcgaa
     1201 ttcggcgacg gcaagttcac cgttgccggc accgatcgca gcaagacctt tggcgagatc
     1261 gcgctggcgg cctacgtgcc gcacaactat ccgctcgaca agctcgagcc cgggctcgaa
     1321 gagaccgcgt tctacgatcc gaccaacttc accttccccg ccggcacgca catctgcgaa
     1381 gtggaagtcg atccggagac cggcgtggtc caggtcgtta acttcagtgc ctgcgacgat
     1441 ttcggcaaca tcatcaatcc gatgatcgtc gaagggcagg tgcacggcgg tgtcgcgcag
     1501 ggcatcggcc aggcgctgct cgagtgctgc gtgtacgaca aggatagtgg tcagctcctc
     1561 accggcagct acatggatta cgcgatgccg cgctcc
//
LOCUS       DATANS010000104         2014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_173634, whole genome shotgun sequence.
ACCESSION   DATANS010000104 DATANS010000000
VERSION     DATANS010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173634"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VHT22_01510"
     CDS             complement(<1..619)
                     /locus_tag="VHT22_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136256.1"
                     /translation="MKRIGNALATLVASAVGATMLGACAAVEAHWPGEPAAVEIYDRT
                     EGRVLPSYTHQGRRYVVGKPGNEYSIRVRNRSGGRVLAVMSVDGVNAISGDTASTAQT
                     GYVLSPHESADIAGWRKSMSNTAAFYFTTLPDSYAARTGRPDNVGVIGVAVFREQPRR
                     VVEQKLERRDAAEPMAKAAKPSSAGALNSEAAAAPQPSLGTGHGRS"
     gene            744..1889
                     /locus_tag="VHT22_01515"
     CDS             744..1889
                     /locus_tag="VHT22_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEX3136257.1"
                     /translation="MPDSSHLPALVAWLAFALAFVFGAVANRVNFCTMGAITDIVHIG
                     DWRRMRMWLLAIAVAIAGAALLEAAGLVDLSKTIYTTPRIAWLSYLVGGFLFGFGMTL
                     ASGCGSKTLIRLGGGSLKSLVVLIVLAISAYMTLKGLFAVWRVNALDVVRWDIAPLGI
                     ARSDLGSFAARLGGGAATRLGLALAIAVAIAVFVFASRDFRSSRELAIGGIVIGAVIV
                     GGWYVSGHVGYVAEDPRTLEEAFVATNSGRAESFSFVAPVAYLLELLLLWSDQSKLVT
                     FGIAGVLGMAAGSAATALATGAFRWEGFASTEDLVNHIAGGVLMGFGGVTALGCTIGQ
                     GLTGISTLALGSFLAFGAIVAGCVAASKYQYWRVQMDDEPRRMKALQ"
     gene            1886..>2014
                     /locus_tag="VHT22_01520"
     CDS             1886..>2014
                     /locus_tag="VHT22_01520"
                     /inference="COORDINATES: protein motif:HMM:NF018391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEX3136258.1"
                     /translation="MIVYDLVCAQQHRFEGWFASTEDYARQHDQTMIRCPVCDDAAI"
BASE COUNT          274 a          631 c          698 g          411 t
ORIGIN      
        1 cacttcggcc gtgcccggtg ccgagactcg gctgtggtgc ggctgccgcc tcggagttga
       61 gcgcgccggc cgaactgggt ttcgcggctt tggccatcgg ctcggcggcg tcgcggcgct
      121 ccagtttctg ctcgaccacc cgacgcggtt gctcgcggaa cacggcgacg ccgatcacgc
      181 cgacattgtc cggccggccg gtgcgcgcgg cgtacgagtc gggaagcgtc gtgaaataga
      241 acgccgcggt gttcgacatg ctcttgcgcc atccggcgat gtcggcgctt tcgtgcggcg
      301 agagcacgta gccagtctgc gccgtcgacg cggtgtcgcc cgagatcgcg ttgacgccgt
      361 cgacactcat caccgcgagc acccgcccgc cgctccggtt gcgcacgcgg atcgagtatt
      421 cgttgcccgg cttgccgaca acatagcgcc ggccctggtg cgtgtagctc ggcagcacgc
      481 ggccttcggt gcggtcgtag atttcgacgg ccgcaggctc gccgggccag tgcgcttcga
      541 cggcggcaca ggcgccgagc atcgtcgcgc cgacggccga ggcgaccagg gtcgctagcg
      601 catttccgat tcgcttcatc ttgtctcctg ttgccgcttg aaaatagcgg tccggactgt
      661 aaacgcgagc ggccgcgaat cggggttaag cgtcggggtt gactggatgg ccgccgtgaa
      721 cgtgcgtgcg ataatcgccg gtcatgccgg attcgagtca tcttcctgca ctcgtcgcct
      781 ggctcgcctt cgcgctggcc ttcgtcttcg gcgccgtcgc caaccgggtc aacttctgta
      841 cgatgggcgc gatcaccgac atcgtgcaca tcggcgactg gcgccggatg cgcatgtggc
      901 tgctggcgat cgccgttgcg attgccggcg cggctttgct ggaagcggcc ggactcgtcg
      961 acttgtcgaa gacgatctac acgacgccgc ggatcgcctg gctttcctac ctggtcggtg
     1021 gattcctgtt cgggttcggc atgacccttg cttcgggctg tggcagcaag acgctgatcc
     1081 ggctcggcgg tggaagcctc aagtccctgg tggtattgat cgtgctggca atatcggcct
     1141 acatgacgct gaagggctta tttgccgtct ggcgcgtcaa cgcgctggac gtcgtgcgct
     1201 gggacatcgc cccgctgggg atcgcgcgct ccgatctggg cagctttgcc gcccgtctgg
     1261 gcggcggcgc cgcgacccga ctgggcctcg ccctggcgat cgcggtggcg atcgccgttt
     1321 tcgtgttcgc cagccgcgat ttccgcagca gccgcgagct cgcgatcggc ggcatcgtca
     1381 tcggggcggt gatcgtcggc ggatggtatg tgtcgggcca tgtcgggtat gtcgccgagg
     1441 atccgaggac gctggaagaa gcctttgtcg ccaccaattc gggacgcgcc gagtcgttca
     1501 gctttgtcgc tccggtggcc tatttgctgg aactgttgct gttgtggagc gatcagtcca
     1561 agctcgttac cttcggcatc gccggcgtgc tcggcatggc ggcgggatcg gccgccaccg
     1621 cgcttgcgac cggggcgttc cgctgggagg gcttcgcatc gaccgaggac cttgtcaacc
     1681 atatcgccgg cggagtgtta atgggatttg gcggcgtcac tgcgctcgga tgcacgatcg
     1741 gccaggggct gaccggcatt tcgaccctgg cgctgggatc gttcctggcc ttcggcgcga
     1801 tcgttgccgg gtgcgtagcg gcatcgaagt accagtattg gcgagtgcag atggacgatg
     1861 agcctcgccg catgaaagcg ctgcaatgat tgtttacgat ttggtttgtg cgcagcagca
     1921 tcggttcgag ggctggttcg cctcgaccga ggattacgcg cgccagcacg accagacgat
     1981 gattcgttgc ccggtgtgcg acgacgccgc gatc
//
LOCUS       DATANS010000105         2400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_173674, whole genome shotgun sequence.
ACCESSION   DATANS010000105 DATANS010000000
VERSION     DATANS010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173674"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VHT22_01525"
     CDS             complement(<1..192)
                     /locus_tag="VHT22_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136259.1"
                     /translation="MGSRPVVGIRLAAVLALAVSLAPAAGAEPGAAVGSISYQSRGGL
                     ITVWPKYAYLVKGPDTVTGN"
     gene            complement(192..1841)
                     /locus_tag="VHT22_01530"
     CDS             complement(192..1841)
                     /locus_tag="VHT22_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX3136260.1"
                     /translation="MMINPRQIAVIAAAVAALSACSKPPDPPTAAPKPKTDAIAAANA
                     AAPAATTTEGLHQVGGIAWQKGDVDAAFAAAKAQHKPVFLYWGAVWCPPCNQVKATIF
                     NRQDFIERSRFFVPVYVDGDSASAQRLGDRFKVSGYPTMILFTPDGGEITRLPGEVEV
                     DQYMRVLAMGMNGARPVKETLAAALSSGSGAALSADDWRMLAYYSWDTDEHTLVAAKT
                     LSPTLERLAKACPATQPDTAARLRLRALVAAATAKDVKPREDKAAAADLTHLLADPRL
                     ARENFDLLTYYADKVVAYITPPKSAARAKLAADWNAVLERFVADPAISTADRLSAISG
                     RIALARLDAPKGSALPDPLLQTVRDEVARADRETTDPYARQAVISAAADALSDADLMA
                     ASDALLEAELKRSHSPYYFMLDLAANAKKRGDKAAALDWEQKAYAAADGPATRLQWGV
                     HYVNALIELAPHDEARIEHAAKSVIGELDPTPDTFYERNRRGLERMGGKLAAWNAQHR
                     HEPAMARIRAQMAAVCAKLPAADPARATCDGVMRSAKGASA"
     gene            2085..>2400
                     /locus_tag="VHT22_01535"
     CDS             2085..>2400
                     /locus_tag="VHT22_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136261.1"
                     /translation="MNTWARVRHELIATILLTLLLLAMAMLSPPVASAADEARLQTNG
                     AVEYRSGGIGKEEADALKQQSADYALTLEFATSRSAEGDTSPGAYIADVRVDIRDAQG
                     RPI"
BASE COUNT          363 a          779 c          835 g          423 t
ORIGIN      
        1 attgccggtc accgtgtcgg ggcccttgac caggtacgca tatttcggcc acacggtgat
       61 caggccgcct ctgctctggt agctgatcga gccaacggct gcacccggct cggcgccggc
      121 cgccggcgcc agcgagacag ccagcgccag cactgccgcg agacggatcc cgacgacggg
      181 cctagatccc actaggcgct cgcgcctttc gcggatcgca tcacgccgtc gcaggtagcc
      241 cgggccggat cggccgccgg caacttggcg cagacggcgg ccatttgtgc gcgtatccgg
      301 gccatcgccg gttcgtgccg gtgctgcgcg ttccacgcgg cgagcttgcc gcccatgcgc
      361 tcgaggccgc gtcggttgcg ctcgtagaag gtatccggcg tcggatcgag ctcgccgata
      421 acgctcttcg ccgcatgctc gatccgcgct tcgtcgtgcg gtgcgagctc gatcagcgca
      481 ttgacgtagt gcacgcccca ctgcaggcgc gtcgccggac cgtcggcggc ggcatacgct
      541 ttttgctccc agtcgagcgc cgccgccttg tcgccacgct ttttcgcgtt ggcggcgaga
      601 tcgagcatga aatagtaggg cgagtgcgag cgcttgagct cggcctcgag cagggcatcg
      661 gatgccgcca tcaggtcggc gtcggagagt gcgtcggcgg ccgcgctgat gaccgcctgg
      721 cgtgcatacg ggtcagtggt ttcgcgatcg gcgcgtgcca cctcgtcgcg gacggtttgc
      781 agcagcgggt ccggcagcgc cgaacctttg ggcgcatcca gcctggccag cgcgatgcgc
      841 ccgctgatcg cggagagccg gtctgccgtc gatatggcgg gatcggcaac gaatcgctcc
      901 agcaccgcgt tccagtccgc tgcgagcttg gcgcgcgcgg cgcttttggg cggcgtaatg
      961 taagcgacga ccttgtcggc gtaataggtc aacaggtcga agttttcgcg cgcaagtcgc
     1021 ggatcggcaa gcaagtgggt gagatcggcg gccgcggcct tatcctcgcg cggcttgacg
     1081 tccttcgcgg tggccgcggc gaccagcgcc cgcagccgca gccgggccgc ggtatccggc
     1141 tgggtggccg ggcaggcttt cgcgagccgt tccagagtcg gcgacagcgt tttggccgcg
     1201 accagcgtgt gttcgtcggt gtcccacgaa tagtaggcga gcatgcgcca gtcgtcggcc
     1261 gacagcgctg cgccgctgcc ggacgacaag gcggcggcga gcgtctcctt gaccggccgc
     1321 gcgccgttca tgcccatcgc cagcacgcgc atgtattgat cgacctcgac ctcgcccggc
     1381 aggcgcgtga tctcgccgcc atcgggcgtg aacaggatca tcgtcggata gccgctgacc
     1441 ttgaaacggt cgccgagtct ctgcgcgctg gcgctgtcgc cgtcgacgta gaccggcacg
     1501 aaaaagcggg agcgctcgat gaaatcctga cggttgaaga tcgtcgcctt gacttgattg
     1561 cagggcgggc accacacggc tccccagtag aggaacaccg gcttgtgctg cgccttcgct
     1621 gcggcgaaag ccgcgtcgac atcgcctttt tgccaggcga ttccgccgac ctgatgcagg
     1681 ccctcggtcg tagttgccgc gggtgccgcc gcattggccg cggcgatggc gtccgtcttg
     1741 ggcttgggcg cggcggtcgg gggatcgggc ggcttcgagc acgcgctcag cgccgcgact
     1801 gcggcagcga ttacggcaat ttgtctgggg ttgatcatca tcgtcttcga cattccaggt
     1861 ggaccgatat tatgccagcc gcccaagttc ggcgcgaagg ccggctccta tgtcctacag
     1921 gcgtttcaga ctccggctga ttcactttgc ggctccgcag gccaagaatc ggtcgggtgg
     1981 cgctgggacg ggcgatgccc atcgcagcca acgcccagcc gaagggagcg accgatgacc
     2041 agcacaggca ggctttcgcg tgtttgcgaa tggacggccc aatcatgaac acctgggcgc
     2101 gggtgcggca tgagctgata gcgacgatct tgctgacctt gctgctgctg gcgatggcga
     2161 tgctttcacc gccggttgcc agcgcggccg acgaggcgag gctgcagacc aacggtgcgg
     2221 tcgagtatcg gtcgggcggc atcggcaagg aggaggcgga tgcgctgaag cagcagtcgg
     2281 ccgactatgc tctgaccttg gaatttgcta cgtcgcgcag cgcggaaggc gacaccagcc
     2341 ccggtgctta cattgccgat gtccgagtcg acatccgcga cgcgcagggc cgcccgatag
//
LOCUS       DATANS010000106         2343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_299029, whole genome shotgun sequence.
ACCESSION   DATANS010000106 DATANS010000000
VERSION     DATANS010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299029"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1844
                     /locus_tag="VHT22_01540"
     CDS             <1..1844
                     /locus_tag="VHT22_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX3136262.1"
                     /translation="AAQKLVEWYILRHPETTASFSRYAAFIAANPEWPSAALLRRRAE
                     ARLWEQRSDAATIRGFMGDRPTSAKGKLALARVLLAEGDRDGAARLARDAWRSDELTE
                     RLEADVLETFRDLLTRDDHRARMDRRIGAKDLAGAKRAAQRLGGDELAIVKACAAVKG
                     KADKAKDTLDDVAIEARQDLGYTLCRIQWMLAQNRIDDAARLVLAASPETMALQATDQ
                     WWRERRSLARKLLDEGKFQTAYDVVRPAATPANEYYRADVHFMCGWIALRYLDNPKTA
                     ATHFAHIDDGATNPIVLARANYWRGRAAEALGQADAMRASYQAAARYPTAYYGQLAHA
                     RLGREPIELRAPSPVLASADTSATDERVRAAEMLYEIGERDVVLYFAADLGEQSSDVA
                     LLEALGELTGRRNDARAMLQIGKPSLGRGLPLDHYAFPTIGIPPHRQLAPEIEHSMIY
                     SVARTESAFDQRDKSAANAVGLMQVTPEAGRDTAKRFKVSYDWDRMVSDPVYNTQIGA
                     AELSALLSEYKGSHIMTFAGYNAGRGRVRDWVKAYGDPRDPKVDPIDWVERIPFSETR
                     NYVQRVIENLAVYRVRFDVSTTATAKTDARVVTQETNAAPGPASPAQ"
     gene            complement(1891..>2343)
                     /locus_tag="VHT22_01545"
     CDS             complement(1891..>2343)
                     /locus_tag="VHT22_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3136263.1"
                     /translation="SIRDSLLALSGIPFAVAGGILGLSIGGLNFSVSAAVGFISLFGV
                     SAMDGILLMSYIRRDIDEGMGTEDAIIRASQTRMRQIFMTGLSACIGLVPAAISTGIG
                     SQVQQPLACVIVGGMLLSPICSLLVIPVLARLWMPTIKETGMREAAVH"
BASE COUNT          444 a          782 c          762 g          355 t
ORIGIN      
        1 cggcggcgca gaagctcgtc gagtggtaca tcctgcgcca cccggagacg acagcgagct
       61 tcagccgcta cgcggcgttc atcgccgcca acccggaatg gccgagcgcg gcgttgctgc
      121 gccggcgcgc ggaggcacgg ctgtgggagc agcgcagcga tgcggcgacg attcgcggtt
      181 tcatgggaga tcggcccacc agcgccaagg gcaagttggc gctggcgcgc gtcctgctcg
      241 cggagggcga tcgggacggc gcggccaggc tggcgcgcga cgcatggcgg tcggacgaat
      301 tgacggagcg cctggaggcg gacgtgctcg agacgttccg agaccttctc acgcgcgacg
      361 accaccgtgc gcgcatggac aggcgcatcg gcgccaagga cctcgccggc gcgaagcgcg
      421 ccgcgcagcg ccttggcggc gacgagcttg cgatcgtgaa ggcttgtgcc gcggtcaagg
      481 gaaaagcgga caaggcgaag gatacgctcg acgatgtcgc catcgaggca cggcaggatc
      541 tcggctacac cctttgccgc atccagtgga tgctcgccca aaaccgcatt gacgatgcgg
      601 cccgcctggt gctggcggcc tcgcccgaga cgatggcgct gcaggccacc gatcaatggt
      661 ggcgcgagcg gcgctcgctc gcccgcaagc tgctcgacga aggcaagttc cagaccgcct
      721 acgatgtcgt ccgtcccgcc gcaacgccgg ccaatgaata ttaccgggcc gacgtccact
      781 tcatgtgcgg ctggatcgca ctgcgctacc tcgacaatcc caagaccgcg gccacgcatt
      841 tcgcccacat cgacgacggc gcgaccaatc cgatcgtgct ggcgcgggcg aactactggc
      901 gcggacgcgc cgccgaagcc cttggccagg cggatgcgat gcgtgcgagc tatcaagcgg
      961 ctgcacgcta cccgaccgcc tattacgggc agctcgcgca cgccaggctt ggccgtgagc
     1021 caatcgagct gcgcgcgccc tcgcccgttc tcgcgtctgc ggatacttcg gcgacggacg
     1081 aacgcgtgcg cgccgccgaa atgctctacg agatcggcga acgcgatgtc gtgctctatt
     1141 ttgccgccga tctcggcgaa caaagctccg acgtggccct gctcgaagcg ctcggcgaac
     1201 ttaccggccg tcgcaacgac gcccgcgcga tgctgcagat aggaaagccc tcgctcggcc
     1261 gcggactgcc gctcgaccat tacgcatttc ccaccatcgg gattccgccg caccgccagc
     1321 tcgcgcccga aatcgagcac agcatgattt attcggtagc gcgcaccgag agcgcgttcg
     1381 accagcgcga caagtctgcg gcaaatgcgg tcggcctgat gcaggtgacg ccggaagcgg
     1441 gacgcgatac cgcaaagcgc ttcaaggtca gctatgactg ggaccggatg gtttccgacc
     1501 cggtctacaa cacgcaaatc ggcgccgccg agctcagcgc gctgctatcg gaatacaaag
     1561 gcagtcacat catgaccttt gccggctaca atgccggcag gggccgcgtg cgggactggg
     1621 tcaaggcgta tggcgatccg cgcgatccca aggtcgaccc gatcgactgg gtcgagcgca
     1681 ttccgttttc cgaaacgcgc aactatgtcc agcgggtgat cgagaatctg gcggtgtacc
     1741 gcgtccgttt cgacgtcagc acgacagcga cggcgaagac cgacgcgcgc gttgttaccc
     1801 aggaaacgaa cgcggcgccg gggccggcga gcccggcaca ataagttcga cgcatcgacg
     1861 ggccatgtgc gccgaatacc ggccggcctg tcagtgaact gcagcctccc gcatcccggt
     1921 ttccttgatc gttggcatcc acagtcttgc cagtaccggg atcaccagca ggctgcagat
     1981 cggggacagc agcattccgc cgacgatgac gcacgccagt ggttgttgaa cctgcgaacc
     2041 gatccccgtc gagatggccg ccgggaccag accgatacac gccgatagcc ctgtcatgaa
     2101 gatttgccgc atccttgttt gggaggcgcg gatgatggcg tcctccgtcc ccatgccttc
     2161 atcgatgtcg cggcggatat aggacatcag caggatgcca tccatcgccg agacgccgaa
     2221 cagcgagatg aagccgaccg cggccgagac gctgaaattc aggccgccga tagagagccc
     2281 gagaattccg ccggctaccg cgaacggaat gcctgacagc gccagcaggc tgtcccgtat
     2341 cga
//
LOCUS       DATANS010000107         5943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_215085, whole genome shotgun sequence.
ACCESSION   DATANS010000107 DATANS010000000
VERSION     DATANS010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215085"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VHT22_01550"
     CDS             <1..527
                     /locus_tag="VHT22_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3136264.1"
                     /translation="YCNDGRFRDALLFTHRGLSGPAILQISSYWNGGDALSIDLLPGS
                     DAHAWLETERRSTARLDNILARRLPRRFAQQWSGAHHAAVPVSALDEKRRHELASLLH
                     AWPVLPSGTLGYNKAEVTLGGVDTRALSSRTMAARELPRLHFIGEVVDVTGWLGGYNF
                     QWAWSSGYAAGQHA"
     gene            complement(537..1052)
                     /locus_tag="VHT22_01555"
     CDS             complement(537..1052)
                     /locus_tag="VHT22_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013951778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX3136265.1"
                     /translation="MRRRLYFLLPDLPSARKTMDDLLLARIEERHIHFLARPGMPMDG
                     LHECNVLQSTDLVHGAQTGLIAGAVLGCVAGGAAAYFLLTESSHMQIPIVIGAALVGA
                     LLGAWFSSMVGSALPNSRLKQFEPMIAEGKLLLMVDVPEHSVEQVKMRVGRDHPEAVS
                     GGTDPHIPAFP"
     gene            1216..2361
                     /locus_tag="VHT22_01560"
     CDS             1216..2361
                     /locus_tag="VHT22_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3136266.1"
                     /translation="MPYRVILLLAVVAFGCHPAVAQVRIGLMVSATGPTAAIGIPQKN
                     TGALLATRIGETTIDYVQLEDGGDTTRAVQNAKKLIAEDRVDAIIGPSTTPNALAILD
                     IISENKVPLLATVGTSSVVEPIDAKRRWVFKTTQNDDLIASALIEHMRRNRVVSVGFI
                     GFNDAYGENWHKVFGALAEKSGIAIVANERYARNDSSVTAQALKLIAARPDAVLIAAV
                     GGPAVLPQTTLYDQGYKGRIYQTHAVATDDFIRLGREKVEGTILAAGSMLVIDDIADS
                     DPIKRVAAGYMSAYEKRFGTRPATFGANTFDAGLLLQRAIPIAVRAAKPGSQEFRVAL
                     RDALEQQQGVVGCQGVFNMSPSNHNGMDERARVLVVVKDNRFRLLAQ"
     gene            2375..2953
                     /locus_tag="VHT22_01565"
     CDS             2375..2953
                     /locus_tag="VHT22_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX3136267.1"
                     /translation="MQRPIVGLLLAAGSGSRFGGDKLLATLADGRPLASAALSALAAG
                     VDHVIAIVRPGDAALEALLGKSGALVAVCPDAAEGIGASLACGVGEVQRRFPEAQGAI
                     IALADMPWLSSSTVASVATALRRGAALAAPTHRGTRGHPVAIGARYFAELQTLSGDEG
                     AKKLLAAHAAEIELIAVDDPGVLRDVDTQSDL"
     gene            complement(2960..5209)
                     /locus_tag="VHT22_01570"
     CDS             complement(2960..5209)
                     /locus_tag="VHT22_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3136268.1"
                     /translation="MAARAPTNDERPAPADALSRRRAIRIGLLAKLNGLAVGLIMATA
                     IGVSVLLVAQQIEAEQARLKTQGQTIVNMLADLSEYAVYTADLSALERLLQSLEGDRD
                     IAYAAVLDDQGHLIASRGYGDAAMPPAPTAELHPGAVESSVRQVRGRRYLELVAPISE
                     TANERAPAAAGAPVAADPPIGYVRLGLNYERNEALLQSNVLGALSVVAALTVIAILAT
                     LLLTRHLLSPIRELMRAAKAVGAGDLGVRVKPTSGDELGMLTRAFNQMTQRLAQSQGE
                     VTGYQRTLEEKVVQRTKELEIAIAQAYKLAQHDILTGLPNRALLNQRLKQILAQAARD
                     HHQVACLFLDFDHFKRINDTLGHDAGDHLLQAVSQRLSHAVRESDTVARLGGDEFVVI
                     LPDLDPERATIEIMAVLTRVRESFLAPFRLGDQTPTLTCSIGVSIYPTDATDPGGLIR
                     QADTAMYSVKDTGRNTYRFYTADMNARVQARLQIETDMRRGLMDDEFFLVYQPQIEMA
                     SGRACGVEALLRWRDPERGIIGPAEFISIAEESGMIQALGARVLRDACRQVTNWHRQN
                     MLLRLSVNLSVQQLQHDSWLSIVEEALRVSGLPPRYLDLEITESVIITHPDKAVSTLI
                     KLKQMGVSITIDDFGTGYSSLSYLARLPIQAVKIDQRFVRGIEQNSSDEAIAQAIIAL
                     SHSLGLRVIAEGVETAAQFEFLKTHGCEEAQGFLISHPLGEAELRAWWQMQDEENRIV
                     GRQVDMWRK"
     gene            complement(5215..>5943)
                     /locus_tag="VHT22_01575"
     CDS             complement(5215..>5943)
                     /locus_tag="VHT22_01575"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX3136269.1"
                     /translation="TPVVEIPVGDTGARLKAAAHEDPDAAIITLGPQAAMRAARDAPL
                     LAAIDCMSTQAGASAQAVPAAIPLEQQLTWLKRLLPGARYIGVLYDPAQNTELVDALA
                     TALRGTDLNPVLAPVATPAMLPAALARLSSSADALLAVPDTTVYKRETVKALLLFSFR
                     HKLPLIGLSEAWVQAGALYALDWDYRELGAFCGRLALRQLPGARVATPTPPHPHVYVN
                     LRSARLFRVHWDESVRGSLERVVE"
BASE COUNT         1006 a         1956 c         1973 g         1008 t
ORIGIN      
        1 tgtactgcaa cgacgggagg tttcgcgacg cgctgttgtt cacccatcgc ggcctgtccg
       61 gcccggcgat ccttcagatc tcgtcgtatt ggaacggcgg cgacgcgctg tcgatcgact
      121 tgctgccggg cagtgacgcg catgcctggc tggaaaccga gcgccggtcc acggcgcggc
      181 tggacaacat cctcgcgcgc cgcctgccgc gccgtttcgc gcagcaatgg agcggggcgc
      241 accacgccgc cgtgccggtc agtgcgctcg acgaaaagcg ccggcacgag ctggcaagcc
      301 tgctccacgc ctggccggtg ctgccgtcgg gcacgctcgg ctacaacaag gccgaggtca
      361 cgctgggtgg cgtcgatacg cgcgcgctgt catcgcggac catggcagcg agagagttgc
      421 cgcggctgca tttcatcggc gaagtcgtcg acgtcaccgg ctggctgggc ggctacaatt
      481 ttcaatgggc gtggtcttcc ggctacgcgg ccggacagca cgcctgaccc ggcgctttaa
      541 ggaaatgccg ggatgtgcgg atcggtgccg ccggagaccg cttcaggatg atcgcggccg
      601 acgcgcattt tcacctgctc gacgctgtgc tcgggcacgt cgaccatcag cagcagcttg
      661 ccttctgcga tcatcggctc gaattgcttc aggcgagaat tgggcagcgc cgagccgacc
      721 atgctcgaaa accatgcgcc gagcagcgcg ccgaccagcg ccgcgccgat gacgatcggt
      781 atctgcatgt gcgaggattc agtcagcaga aaataggcgg cggctccgcc ggccacgcag
      841 ccaagcacgg ctccggcgat cagacctgtt tgcgcaccgt gcacgagatc ggtcgattgc
      901 agcacattgc attcgtgcag cccgtccatc ggcatcccgg gcctggcgag gaagtggatg
      961 tgacgctcct cgatacgtgc cagcaggaga tcgtccatcg tcttgcgtgc actcgggagg
     1021 tcgggtagca ggaaatagag tctgcgtctc atcgcatgcc tccatggcgg agtaactccc
     1081 gatatcactc tactgccgcc acgccgaggg cgcaagccat cggcgccagt cgagctcgcg
     1141 cgcgaccgcg gcggtttgcg cgcgcgcacg gcagggcata gcattcgcat ctcattgtcg
     1201 ctgccggagc acgccatgcc gtatcgcgtc attttgctgc tcgccgtggt cgcgttcggc
     1261 tgccaccccg ccgttgcgca agtcaggatc ggcctgatgg tttcggcaac cggtccgacc
     1321 gcggcgatcg gcattccgca gaagaacacc ggcgcgcttc tcgctacgcg gataggcgag
     1381 acgacgatcg actatgtgca actcgaggac ggcggcgata cgacccgcgc cgtgcagaac
     1441 gccaaaaagc tgatcgccga agatcgggtt gacgccatta tcggtccgtc gacgacgccg
     1501 aatgcgctcg cgatcctgga catcatcagc gagaacaagg tgccgctgct ggcgaccgtc
     1561 ggcacatcat cggtggtcga accgatcgac gcaaagcgac gctgggtgtt caagaccacg
     1621 cagaacgacg acctgatcgc ctcggcgcta atcgagcata tgcgcaggaa ccgggtcgtg
     1681 agcgtcggct tcatcggctt caacgacgcc tatggcgaga attggcacaa ggtattcggt
     1741 gcactcgccg aaaagtcagg cattgcgatc gttgccaatg agcgctatgc gcgcaacgat
     1801 tccagcgtca ccgcgcaggc gctgaagctc attgccgcca ggcccgacgc tgttctgatc
     1861 gcggccgtcg gcggacccgc ggtgctgccg cagaccacac tctacgacca gggctacaag
     1921 ggccgcatct accagacgca tgcggtggcg accgatgact tcatcaggct gggcagggaa
     1981 aaggtcgaag gaacgatact cgccgccggg tcgatgctgg tgatcgacga catcgccgat
     2041 tccgatccga tcaagcgcgt cgcggccggc tatatgagcg cgtacgagaa gcggttcggg
     2101 acccgccccg caaccttcgg cgccaatacc ttcgatgccg gcttgctgct tcagcgggcg
     2161 attcccattg cggtgcgcgc ggcgaagccc ggcagccagg agtttcgcgt cgcgctgcgc
     2221 gacgcgctcg agcagcagca gggcgtcgtc ggctgccagg gcgtattcaa catgagcccg
     2281 agcaaccaca acggcatgga cgagcgggcg cgcgtgctgg ttgtcgtcaa ggacaacagg
     2341 ttcaggcttc tggcgcagta gcatcggact cgcgatgcaa cgaccgatcg tcggcttgct
     2401 gcttgccgcg ggcagcggct cgcgtttcgg cggcgacaag ctgctggcca cgctcgccga
     2461 cggccggcca ctggcgtcgg ccgcgctgtc cgcgctagcc gctggcgtcg accatgtcat
     2521 cgccatcgtg cgtcccggcg atgcggccct cgaggcgctg ctgggcaagt ccggtgcgct
     2581 ggtcgccgtc tgtcctgacg cagccgaggg catcggcgcc agcctcgcct gcggcgtggg
     2641 cgaagtgcag cgacgctttc cagaagcgca aggcgcgatc atcgcgctcg ccgatatgcc
     2701 ctggctgtcg tcgtcaaccg tcgcgtcggt cgcgactgca ttgcggcgcg gcgcggcgct
     2761 ggcggcgccc acgcatcgcg gaacgcgagg ccatccggtg gcgatcggcg cacgctattt
     2821 cgccgagctg cagacgcttt ccggcgacga aggcgcaaaa aagctgctgg cggcgcacgc
     2881 cgcagagatc gagctgattg ccgtcgacga tccgggcgtg ctgcgcgatg tcgatacgca
     2941 gtcggatctt tgacccgggt cacttcctcc acatgtcgac ctgccggccg acgatgcgat
     3001 tctcctcgtc ctgcatctgc caccacgcgc gaagctcggc ttcgccgagc ggatgcgata
     3061 tcaggaagcc ctgggcctcc tcgcagccgt gcgtcttgag aaactcgaac tgggccgcgg
     3121 tctcgacgcc ttccgcgata acgcgcaagc caagactgtg tgacagcgcg atgatcgcct
     3181 gagcgatggc ctcgtcgctc gagttctgct cgatgccgcg cacgaagcgc tggtcgatct
     3241 tgaccgcctg aatcggcagg cgagccaggt acgacaggct cgaataaccg gtgccgaaat
     3301 cgtcgatcgt gatcgatacg cccatttgct tcagcttgat cagcgtcgag accgccttgt
     3361 ccgggtgcgt gatgatcacg ctttcggtga tctcgaggtc gagatagcgc ggcggcagtc
     3421 cgctgacccg gagtgcctcc tcgacgatcg acagccagct gtcgtgctgc agctgctgca
     3481 ccgaaagatt gaccgacagg cgcagcagca tgttctggcg gtgccagttg gtcacctgcc
     3541 ggcaggcgtc gcgcaacacg cgcgcgccga gcgcctggat catcccggat tcctcggcga
     3601 tcgagatgaa ttcggccggt ccgatgatgc cgcgctccgg atcgcgccag cgcagcaggg
     3661 cttcgacccc gcaggcgcgc ccgctggcca tctcgatctg cggctggtag acgagaaaga
     3721 actcgtcgtc catcagaccg cgacgcatat cggtctcgat ctgcaaccgg gcctgcacgc
     3781 gcgcgttcat atctgcggtg tagaagcggt aagtattgcg cccggtgtcc ttgaccgaat
     3841 acatcgcggt atcggcttgc cggatcagac cgcccggatc ggtcgcatcg gtcggataga
     3901 tagacacgcc gatcgagcag gtcaaagtcg gcgtctggtc gccgagccgg aacggcgcga
     3961 ggaacgattc gcgcacccgc gtcagcaccg ccatgatctc gatcgtcgcc cgctccgggt
     4021 cgaggtcggg aagaatgacg acgaattcgt cgcctccgag ccgcgccacc gtgtcggact
     4081 cgcgcaccgc gtggctcagc cgttgggaca ccgcttgcag aaggtggtcg ccggcgtcgt
     4141 ggccgagcgt gtcgttgatg cgcttgaagt ggtcgaaatc gagaaacagg cacgcgacct
     4201 gatgatgatc gcgcgccgcc tgcgcgagga tctgcttcag gcgctggttg agcagagcgc
     4261 gattgggcag cccggtcagg atgtcatgct gcgcaagctt gtacgcctgc gcgatcgcga
     4321 tctcgagctc tttggtccgc tgcacgactt tttcttccag cgtacgctgg tatccggtca
     4381 cttccccctg cgactgcgcg agccgctgcg tcatctgatt gaacgcgcgc gtcagcatgc
     4441 cgagctcgtc gccggacgtc ggtttcaccc gcacgcccag gtcgccggcg ccgaccgcct
     4501 tcgctgcgcg catcagctcg cgtatcggcg acagcaggtg cctggtgagc aacagcgtgg
     4561 cgaggatagc gatcaccgtc agcgcagcga cgaccgacaa tgcgccgagg acgttgcttt
     4621 gcaggagcgc ctcattgcgc tcgtagttca agcccaggcg cacgtagccg atcggaggat
     4681 cggcggcaac aggcgcgccg gccgccgcgg gcgctcgctc gttcgccgtt tcggagatcg
     4741 gtgcgaccag ctcgagataa cgccgccccc ttacctggcg cacgctgctc tcgacggcgc
     4801 ccgggtgcag ctcggccgtc ggcgccggcg gcatggcagc gtcgccatag cctcgcgacg
     4861 cgatcaggtg accctgatcg tcgagcaccg cggcatatgc gatgtcgcgg tcgccttcga
     4921 ggctttgcag cagccgctcg agcgcgctca aatcggccgt atagaccgca tactcggaca
     4981 gatcggccag catgttcacg atggtctgcc cttgcgtttt cagacgcgcc tgctcggctt
     5041 cgatctgctg cgcgaccagc agcacgctga cgccgatggc ggtcgccatg atcaatccga
     5101 cggcaaggcc attgagcttg gcgagcaagc cgatgcgtat cgcgcgccga cgcgagaggg
     5161 cgtcggccgg ggcggggcgc tcgtcgttgg tcggtgcccg ggccgccatc gtcgctattc
     5221 cacgacccgc tctaacgacc cgcgcaccga ttcgtcccag tgcacgcgga acaaccgggc
     5281 actgcgcagg ttgacataga cgtgtgggtg cggcggagtc ggcgttgcga cccgggcgcc
     5341 gggcaactgc cgcagcgcga gccggccgca gaacgcgccg agctcgcggt aatcccagtc
     5401 gagcgcgtag agcgcgccgg cctgcaccca cgcctccgac aatccgatca gcggcagctt
     5461 gtgccggaaa gagaacaaca gcagcgcctt caccgtctct cgcttgtaga ccgtagtgtc
     5521 gggcacggcg agcagtgcgt cggccgagct ggacaggcgc gcgagcgccg caggaagcat
     5581 cgccggcgtc gcaacgggcg cgagcaccgg attgagatcc gtgccccgca acgccgtggc
     5641 aagcgcatcg acgagctcgg tgttctgcgc cggatcgtac agcaccccga tatagcgtgc
     5701 gcccggcagc aatcgcttga gccaggtgag ttgctgctct aatggaatcg cggcggggac
     5761 cgcctgtgcg ctcgcgcccg cttgcgtcga catgcagtcg atcgcggcga gcaagggcgc
     5821 gtcgcgggct gcgcgcatcg ctgcttgcgg gccgagggtg atgatggcgg cgtcgggatc
     5881 ttcatgtgcc gcggccttca gacgcgctcc ggtatcgccg accggaatct ccacaacagg
     5941 ggt
//
LOCUS       DATANS010000108         1732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_131393, whole genome shotgun sequence.
ACCESSION   DATANS010000108 DATANS010000000
VERSION     DATANS010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131393"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VHT22_01580"
     CDS             <1..752
                     /locus_tag="VHT22_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3136270.1"
                     /translation="MQRELASMLHQQSRLAALGLAVSKINHDLRNLLASAQLFSDQLS
                     TSPDPKVQRFAPKLMRALERAIAFCQSTLSYGGAQEAPPERKTIEVEPLIEEVHEALG
                     LGLDVPIRWIVAVERGLTVEADHDQLFRVLVNLARNAMQALETRGARDPARDQIRITG
                     RREGSVAVIEISDTGPGVPEKAQAHLFQAFQGSVRSGGTGLGLAIAAELVRAHGGEIR
                     LVPGTIGATFSISIPDRAVDLNSRRGERASA"
     gene            829..>1732
                     /locus_tag="VHT22_01585"
     CDS             829..>1732
                     /locus_tag="VHT22_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HEX3136271.1"
                     /translation="MVQPVNLLAVATAVPPHKLEQTAVTAAARHAYARTFARYPKLAD
                     VFVNVGIDSRYSVCPLEWLSEPHNLAERTNVYLESASELFVRTARAALDQAGISARDV
                     DVVVTVSSTGIATPSIEARVAPEMGFRPTAMRVPVFGRGCAGGVSGLTLGARLARAEP
                     GEVILVVVVELCTLACRIDRGSKADVISSALFGDGAAAAVLRAGPPTGELRIGAAAEH
                     TWPRTLDIMGWMIDPVGFRVMLSRSLPRFVEQKLAAPVRRFVKAAKLQAPHLICHPGG
                     AKVLDAIEVALELQKGTLRDERDVL"
BASE COUNT          292 a          562 c          574 g          304 t
ORIGIN      
        1 cgatgcagcg cgagctcgcc tcgatgttgc atcaacagag caggctggca gcgctcggcc
       61 ttgccgtgtc gaagatcaat cacgacctgc gcaatttgct ggcctcggcg cagcttttct
      121 ccgatcagct gtccacctcg cccgatccga aagtgcagcg cttcgcgccg aagctgatgc
      181 gcgcactcga acgcgcgatc gcgttctgcc agtcgacgct ctcttacggt ggtgcacagg
      241 aagcgccgcc cgagcgcaag accatcgaag tcgagccgct gatcgaggaa gtgcacgagg
      301 cgctcggcct cggcctcgat gttccgatcc gctggatcgt cgccgtggag cgcgggctga
      361 cggtcgaagc cgatcacgat cagttgttcc gcgttctcgt caacctcgcg cgcaatgcca
      421 tgcaggcact ggaaacgcgc ggcgcgcgcg atcccgcccg cgatcaaatt cgtatcaccg
      481 gacgacgcga aggcagcgtc gcagtcatag aaatatccga caccggcccg ggcgtgccgg
      541 aaaaagccca ggcccatctg ttccaggcgt tccaggggtc ggtgcgatcg ggaggcacag
      601 gtctcggcct ggccatcgcg gcggaactcg ttcgcgcgca tggcggcgaa atccgccttg
      661 tgccggggac aatcggcgcc acattcagca tctcgatccc cgaccgcgcc gtcgacctca
      721 attcacgacg cggtgaacgc gccagcgcct gacgaccgga agaggtagcc gccggcggtg
      781 ttcttcgcct aggggcgggc ataacatttc gcataaagga aaatttatat ggtgcagccg
      841 gtgaatctgc ttgcggttgc gactgcggtg ccgccgcaca agcttgagca gacggccgtc
      901 accgcggcgg cgcggcacgc ctatgcgcgc acatttgcgc gctatccgaa actcgccgac
      961 gttttcgtca atgtcggaat cgacagtcgc tattcggtgt gtccgctcga atggctcagc
     1021 gagccgcaca atctcgccga acgcaccaac gtctacctcg agagcgcgag cgaattgttc
     1081 gtgcgcacgg cacgggccgc gctcgatcaa gccggcatct ccgcgcgtga cgtcgatgtg
     1141 gtcgtcacgg tgtcctcgac cggcatcgcg acgcccagca ttgaagcacg cgttgcgccg
     1201 gagatgggat ttcggccgac ggcgatgcgc gtgccggtgt tcggccgcgg atgcgctgga
     1261 ggggtgtccg gattgacgct cggtgcacgg ctcgcgcgcg cggagccggg cgaagtcatt
     1321 ctcgtggttg tggtggagct gtgcacgctg gcgtgccgca tcgaccgcgg atcaaaggcc
     1381 gatgtgatct cctcggcgct tttcggcgac ggcgcggcgg ctgccgtgct gcgcgccggc
     1441 ccgccgacgg gagaactgcg catcggcgcc gcggccgaac atacctggcc gcgcacgctc
     1501 gacatcatgg gctggatgat cgatccggtc ggcttccgcg tgatgctgtc gcgctcactg
     1561 ccgcgcttcg tcgaacagaa actggccgcg ccggtgcgtc gcttcgtcaa ggccgcaaag
     1621 ctgcaggcgc cgcatctgat ctgtcatccg ggcggtgcga aagttctcga cgcgatcgaa
     1681 gtggcgctcg agctgcagaa gggtaccttg cgtgacgagc gcgacgtgct gc
//
LOCUS       DATANS010000109         2398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_257613, whole genome shotgun sequence.
ACCESSION   DATANS010000109 DATANS010000000
VERSION     DATANS010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257613"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VHT22_01590"
     CDS             <1..293
                     /locus_tag="VHT22_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX3136272.1"
                     /translation="PEVVWIDGNQCPPLDYPSRAIVDGDRLVAAIAAASILAKTTRDA
                     MLVDLDRSYPLYGFARHKGYSTPEHLAALALHGPCAAHRRWFGPVRQTGFDF"
     gene            293..1012
                     /locus_tag="VHT22_01595"
     CDS             293..1012
                     /locus_tag="VHT22_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX3136273.1"
                     /translation="MARSASRKRPAALLFDVFGTVVDWRGSVKKELARLGRSHGVRAD
                     WGEFADAWRAGYRPAMDRVRRGELPWQPLDSLHLRMLRGLVDRFGLELDAREIAALNL
                     VWHRLSAWRDVRAGLRRLKRGCLIGTLSNGNMALLVELSKAARLPWDCILSAELFRHY
                     KPDPEVYLGAAALLALDPAQVMLVAAHKDDLAAARRCGLQTAYVHRPLEFGPDTAHGG
                     PDRRSTLNAGDFEDLADQLGL"
     gene            1097..1378
                     /locus_tag="VHT22_01600"
     CDS             1097..1378
                     /locus_tag="VHT22_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEX3136274.1"
                     /translation="MIHCVLHDPNDSVAVVVVEGIKAGQTLTGLILDEDRTIDLRCAQ
                     DIPLGHKVALKDLNVGDTVIKYGVDIGKVVAPIKAGQHAHVHNIKTKRW"
     gene            1535..>2398
                     /locus_tag="VHT22_01605"
     CDS             1535..>2398
                     /locus_tag="VHT22_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEX3136275.1"
                     /translation="MQKSFWGYRRENGRVGVRNHVIVLPVDDLSNAAAEAVAHNVKGV
                     LAIPHPYGRLQFGPDLDLHFRTLIGAGSNPNVAAVVVIGIEEGWTQKLVDGIAETGKP
                     VSGFGIELHGDHDTIMRASKAARQFMQAASEQQRVECSLDELWVSTKCGESDTTSGCG
                     ANPTVGDAFDKLYAAGSTLVFGETTELTGGEQIVAARCRDDKVRAEFMRVFDRYQEVI
                     NRHKTTDLSDSQPTKGNIAGGLTTIEEKALGNIQKIGKKCMIDGVLDKAETPSGPGLW
                     FMDSSSAAAEMV"
BASE COUNT          427 a          752 c          784 g          435 t
ORIGIN      
        1 cgccagaagt cgtctggatc gacggcaatc agtgtccacc gctcgactac ccgtcgcggg
       61 cgatcgtcga cggcgatcgc ctggttgccg cgatcgccgc ggcctcgata ctcgccaaga
      121 cgacgcgcga tgcaatgctg gtggacctcg accgcagtta cccgctgtac ggattcgccc
      181 ggcacaaggg ctattcaacg ccggagcatc tcgctgccct ggcgctgcat ggcccgtgcg
      241 cagcgcaccg gcgctggttc ggtccggtcc gccagaccgg attcgatttc tgatggcgag
      301 gtcggcgtcg cgcaagcgcc cggcagcgct tttgttcgac gttttcggca ccgttgtcga
      361 ttggcgcggc agcgtcaaga aggagctcgc gcgtctcggg cgatcgcatg gcgtgcgcgc
      421 tgactggggc gagttcgccg atgcctggcg cgccggctat cgtccggcaa tggatcgggt
      481 gcgtcgcggc gaactgccat ggcagccgct cgacagcctg catctaagga tgttgcgcgg
      541 gctggtcgat cgcttcggtc tcgagctcga cgcccgcgag atcgccgcgc tcaacctggt
      601 ctggcaccgg ctcagcgcct ggcgcgatgt tcgggcgggt ctgcgccgtc ttaaacgcgg
      661 ctgcctgata ggcaccttgt ccaatggcaa catggcattg ctggtggagc tgtcgaaagc
      721 cgcgcgcctg ccgtgggact gcatcctttc ggcggagctt ttccggcact ataagcccga
      781 tcccgaggtc tatctcggtg ccgccgcgct cctcgcgctc gacccggccc aggtcatgct
      841 ggtcgctgcg cacaaggacg atctggcggc cgcgcggcgc tgtggcttgc agaccgcgta
      901 tgtgcaccgt ccgctggaat tcggtccgga cacggcgcat ggcggccccg accggcgtag
      961 cacgctcaat gccggggatt tcgaggacct cgccgatcag ctcgggttgt aggcgcgcgc
     1021 aatatgctat gtttgagccc gcagcggcgc tcgatcctgt gccgccgcga gtccattcga
     1081 agcaggagat acggctttga ttcactgtgt attgcatgac cctaacgatt cggtcgccgt
     1141 cgtcgttgtt gaaggcatta aagcaggaca aacgctgacc gggctgatcc tcgatgagga
     1201 ccggacgata gatttgcgct gcgcgcaaga cattccgctc ggacacaagg tcgctttgaa
     1261 agatctgaac gtaggcgata ccgtgatcaa gtatggcgtc gatatcggca aggtcgtcgc
     1321 gccgatcaag gccggccaac acgcgcacgt gcacaacatc aagaccaagc gctggtgacg
     1381 agcgtttgcc gccgcgcgcg acgcgacgct tctccccacg ctgcggtgct gatgtactga
     1441 agccgtacat ttcgctccgc gcgccgatcg cgcctggcct ggtgtccgca tgctggctcg
     1501 tcgcgaccca ctattgttta gcaaggaatc attgatgcaa aaaagcttct ggggataccg
     1561 ccgcgagaat ggccgcgtcg gtgtacgcaa ccacgtgatc gtgctgcccg tcgacgatct
     1621 gtccaatgcc gcggccgagg cggtcgcgca caatgtcaag ggcgtgctgg cgatcccgca
     1681 tccgtatgga aggctgcagt tcggccccga cctcgacctg cactttcgca cgctgatcgg
     1741 cgcggggagc aatccgaacg ttgccgcggt cgtcgtcatc ggtatcgaag agggatggac
     1801 ccaaaagctt gtcgacggga tcgccgaaac cggcaagccg gtcagcgggt tcggtatcga
     1861 gctgcacggc gatcacgaca cgatcatgcg ggcgtccaag gccgcgcgcc agttcatgca
     1921 ggcggccagc gaacagcaac gggtcgaatg cagcctcgac gagctctggg tatcgaccaa
     1981 gtgcggcgaa tctgacacga cctcggggtg tggcgccaac cccacggtag gcgatgcatt
     2041 cgacaagctc tatgcagcag ggtccacgct ggttttcggc gagacgaccg agcttaccgg
     2101 tggcgagcag atcgttgccg cgcgttgccg tgacgacaag gtccgcgccg agttcatgcg
     2161 cgtgttcgac cgctatcagg aggtcatcaa ccggcacaag accaccgatc tgtcggattc
     2221 gcagccgacc aagggcaaca tcgccggcgg actgacgacg atcgaggaaa aggcgctggg
     2281 caacatccag aagatcggca agaaatgcat gatcgacggc gtgctcgaca aggcggaaac
     2341 gccttccggg cccggcctct ggttcatgga cagctcgtcg gccgccgcgg agatggtg
//
LOCUS       DATANS010000110         2195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_760891, whole genome shotgun sequence.
ACCESSION   DATANS010000110 DATANS010000000
VERSION     DATANS010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760891"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VHT22_01610"
     CDS             complement(<1..573)
                     /locus_tag="VHT22_01610"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX3136276.1"
                     /translation="MLKLTRFVFAALALAGCAAPPPSMQHTDLPVVVRASPNFDDRRP
                     NYVILHYTSSADSERALRTLTDPASKVSAHYLIARDGRIYALVDERVRSWHAGVSSWG
                     GHRDVNSAAIGIELDNDGKEAFAEPLIDRLYALLADLKARYLIPTANFIGHSDVAPRR
                     KVDPGRLFPWRELAARGYGLWCDPPYRVPPD"
     gene            complement(594..1298)
                     /locus_tag="VHT22_01615"
     CDS             complement(594..1298)
                     /locus_tag="VHT22_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX3136277.1"
                     /translation="MNVLAACARYRFITALLACAFAGAAYAQVAPGGAEIAAYTGLFA
                     AAARGDAAEIGRLAASGADVNAREGYGRTPLHVATFGRHREAIAALAKARADLNAKER
                     DRYDAVTIAAVADDVATLKLLLQLGASAKQVTSRYDGTALIAAAHLGHDEVVKLLIDA
                     GAPLDHVNNLGWTALIESIVLGQGGPRHVATLTALVRAGANVNLADRNGQSPLQLARQ
                     RRYQAMIAILEQAGAK"
     gene            complement(1298..>2195)
                     /locus_tag="VHT22_01620"
     CDS             complement(1298..>2195)
                     /locus_tag="VHT22_01620"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HEX3136278.1"
                     /translation="EAVVKSGVQLKGNVLLAFVGGEIERHAVIHYQGATYRGGGCGTK
                     HFVNNGGIADMAVVGEPTRMMLINEHVGSVGVRITTRGKPAPLRVADHGDDAIKKMRV
                     LIDVFDDFATEYAGRHKYKNCSSSVHMHSIEGGWPYRCNRVPFFCHVFLEFRLLPNQE
                     INEVPLEVERLLAMVRARRPGIEFDTEFFVTLPPAQDCSTSVVAQRMRDAHRAVTGGV
                     PEEGVGLFYSDASHLQAYGIPAVNYGPSGRTVTGKENWDPDVGEHLAIDDLTKTARVY
                     TSLILDVCSKSREELGLKVLKL"
BASE COUNT          384 a          756 c          683 g          372 t
ORIGIN      
        1 atccggcggc acgcggtacg gcggatcgca ccacaggccg tagccgcgcg cggcgagctc
       61 gcgccacgga aaaaggcgtc cggggtcgac cttgcgccgc ggcgccacgt cgctgtggcc
      121 gatgaaattc gcggtcggaa tcaaatagcg cgctttgaga tcggcaagca gtgcgtaaag
      181 cctgtcgatc agcggttccg caaacgcctc tttgccgtcg ttgtcgagct cgatgccgat
      241 cgccgccgag ttcacgtcgc gatggccgcc ccaagatgaa acgcccgcat gccaggagcg
      301 cacgcgctca tcgaccagcg cgtaaatccg gccgtcgcgc gcgatcaggt aatgcgcgct
      361 caccttgctc gccggatcgg tcagcgtgcg caaggcgcgc tcggaatcgg cgctgctcgt
      421 gtaatgcagg atcacgtaat tgggccgccg gtcgtcgaag ttgggtgacg cccgcacgac
      481 taccggcaga tcagtgtgct gcatggacgg cggcggcgcc gcgcacccgg caagcgcgag
      541 cgccgcaaaa acgaagcgcg taagcttgag catcctcaat cctcagtccc gatctacttc
      601 gcgcccgcct gctcgagtat cgcgatcatc gcctgatagc ggcgctggcg cgcgagctgc
      661 aaaggcgact gcccgttgcg gtcggcaaga tttacattgg cacccgcccg cacgagcgcg
      721 gtcagcgtcg cgacgtggcg cggcccgccc tgcccgagca ctatcgattc gatcagcgcc
      781 gtccagccga gattgttcac gtgatcgagc ggcgcgccgg cgtcgatcag cagcttcacc
      841 acttcgtcgt gacccaggtg cgccgccgcg atcagcgcgg tgccgtcgta gcggctcgta
      901 acctgcttcg cgctcgcgcc cagttgcagc agcagtttca gcgtcgccac atcgtcggcg
      961 acggcggcta tcgtcaccgc atcgtagcgg tcgcgctctt tggcgttcaa atcggcgcgg
     1021 gctttcgcga gcgccgcaat cgcctcgcga tggcggccga acgtcgcgac gtgcaaaggc
     1081 gtgcggccgt acccttcgcg cgcgttgacg tccgcgccgc tcgcggccag gcggccgatt
     1141 tccgccgcgt cgccgcgcgc cgcggcggcg aacaagccgg tataagccgc tatttcagcg
     1201 ccgccaggcg ctacttgcgc atacgccgcg ccggcaaagg cacaagcaag cagcgccgta
     1261 ataaagcggt agcgggcaca cgccgccagc acgttcatca caatttcaga accttcaggc
     1321 cgagctcttc acgcgacttg ctgcacacgt cgagtatcag cgacgtataa acccttgccg
     1381 tcttcgtgag atcgtcgatc gcgagatgct cgccgacatc cgggtcccag ttttccttgc
     1441 ccgtcacggt acgcccgctg gggccgtagt tcaccgccgg aatgccgtaa gcctgcagat
     1501 ggctcgcgtc ggagtagaac aggccaacgc cttcctcggg cacaccgccc gtgacggcgc
     1561 ggtgcgcgtc gcgcatgcgc tgcgccacca cggaagtcga gcaatcctgc gccggcggca
     1621 gcgtcacgaa aaattccgta tcgaattcga tgcccggacg ccgggcgcgc accatcgcga
     1681 gcagacgctc gacttcgagc gggacttcgt taatctcctg gttgggcagc agcctgaact
     1741 ccagaaacac gtggcagaaa aacggcaccc ggttgcaccg gtacggccag ccgccttcga
     1801 tcgaatgcat gtgcaccgac gacgagcaat tcttgtattt gtggcggccc gcatactcgg
     1861 tcgcaaaatc gtcgaacacg tcgatcagca cgcgcatctt cttgatcgcg tcatcgccgt
     1921 gatcggcgac ccgcagcggc gccggcttgc cgcgcgtggt gatgcgcacg ccgaccgagc
     1981 ccacatgctc gttgatcagc atcatgcgcg tcggctcgcc gaccaccgcc atgtccgcga
     2041 tgccgccgtt gttgacgaaa tgcttggtgc cgcaaccgcc gccgcgatac gtcgcgccct
     2101 ggtagtggat caccgcgtgg cgctcgattt caccaccgac gaacgcgagc agcacgttgc
     2161 ctttcaattg cacgccggat ttgacgaccg cttcg
//
LOCUS       DATANS010000111         9025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_340915, whole genome shotgun sequence.
ACCESSION   DATANS010000111 DATANS010000000
VERSION     DATANS010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 9025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9025
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340915"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VHT22_01625"
     CDS             complement(<1..433)
                     /locus_tag="VHT22_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3136279.1"
                     /translation="MSKFTHLGVLTALAAVVVAGSVAAAPVTLRASHQFPGGKGDVRD
                     EMVQIIARDAKAAGVDLDVQVYPGASLFKPNEQWNAMVNGQLDISSFPLDYASGKVRE
                     FGATLMPGLVRNHERAARLGNSKFMKDIRAKIESNGVIVLSD"
     gene            complement(524..3304)
                     /gene="fdhF"
                     /locus_tag="VHT22_01630"
     CDS             complement(524..3304)
                     /gene="fdhF"
                     /locus_tag="VHT22_01630"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEX3136280.1"
                     /translation="MNAPVEAALAAELIEFRLNGEAASARPDETIIEAAKRHGVEIPH
                     LCYKPGMRPDGNCRACMVEIKGERVLAPSCCRKPTAGMEVSSDSPRAVHSQKIIVEML
                     AADVPVRVYKLDSELEQWKRWLGLGAPRFAARAQPKADLSHPAIAVNLDACIQCTRCV
                     RACREEQVNDVIGYAFRGAHSAIVFDLGDPMGASTCVACGECVQACPTGALAPANEAY
                     LVVADKVVPSVCPYCGVGCQLDYHVKDNEIVRVEGRDGPANHERLCVKGRYGYDYVHH
                     PQRLTRPLIRKSGVPKTADFVMDPADPLAVFREASWEEALQVAGGTLKRIRDEHGGKA
                     LAGFGSAKGSNEEAYLFQKLVRTGFGSNNVDHCTRLCHASSVAALLEGIGSGAVSNPV
                     MDVMKADVVLLIGANPVVNHPVAATWIKNAVRNGTRLILADPRRSELARHATHYLQFK
                     PDTDVALLNAMMHVIVEEGLVAQSFVADRTSGFEEIRNNVAGFSPEAMAPVCGVPAAT
                     IREVARMYAKSRGSMILWGMGISQHVHGTDNARCLIALSMMTGQVGRPGTGLHPLRGQ
                     NNVQGASDVGLIPMMYPDYQRVDNAGTRSRFEQVWGTTLDPQPGLTVVEIMDAVHAGQ
                     IRGMYIMGENPAMSDPDVSHARQALAELEMLVVQDIFLTETGYLADVILPASAFPEKT
                     GTFTNTDRSVQLGRQALQPPGEARQDLAIIVAMARTLGLDWHYEHPSQVFEEMRRTMP
                     SIAGITWDRLEREHAVTYPCEHEGDPGQRVVFTEDFPTANGKGRFVPASIIPAAERPD
                     GEYPFVLITGRQLEHWHTGSMTRRARVLDTIEPSPVASINPDDLAKLGVDPGGTITVA
                     SRRGSIALSARADEGTPRGAVFIPFCYYEAAANLLTNPVLDPFGKIPEFKYCAVRVSA
                     GGATAELVGYDG"
     gene            complement(3408..5102)
                     /locus_tag="VHT22_01635"
     CDS             complement(3408..5102)
                     /locus_tag="VHT22_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX3136281.1"
                     /translation="MNEHAIVDISGLDRKRRARAHPKGRPVEPQALAEIRVLLGEAPR
                     RRDLLIEFLHRIQDRYGHISGGHIVALAEEMKLARTEVYEVATFYHHFDVVADGEAPP
                     PPLTVRVCETLSCQMAGADTLLEALQRAHASGIRVVGAPCIGRCEHAPAAVVGRHPVD
                     EATPEKIAAAIADKRIEAEPPPYIDLAAYRAGGGYRTIAACVGGERTVEAVTAALDES
                     GLRGLGGAGFPAGRKWKIVRAEPAPRLMAVNIDEGEPGTFKDRYYLERDPHRFLEGVL
                     IAAWAVGIADVYIYLRDEYAGCRAVLEREISALEADPPCPLPSLHLRRGAGAYICGEE
                     SAMIESIEGKRGMPRLRPPYVAQVGLFGRPTLEHNMETLYWIRDILEKGGAWFGGQGR
                     NGRKGLRSFSVSGRVAKPGVHLAPAGITVRELIAEYCGGMLPGHEFYGYLPGGASGGI
                     LPASLADVPLDFDTLQPHGCFIGSAAVIVLSQHDRARDAALNLMRFFADESCGQCTPC
                     RVGTEKAVGLLSKPKWDTALLAELSQAMADASICGLGQAAPNPIACVVKYFPQELA"
     gene            complement(5148..5912)
                     /locus_tag="VHT22_01640"
     CDS             complement(5148..5912)
                     /locus_tag="VHT22_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3136282.1"
                     /translation="MTMATHIDAPTKPRQPRTRSPAHPQAAARTLALQPITTNVSFRD
                     QAYSAIKQAIMDADIYAHREELRLDERQLSQALGVSRTPIREAMTLLEQEGFLRTVSR
                     RGVFIVRKTKKQIIEMIEMWAALESMAARLATLNASDQDIGALRHMFDEFRNSTPAEH
                     INEYSDANIAFHQAIIRLSGSHLMGKTIDDLFLHVRAIRRMTISQSDRASRSIIDHMR
                     IIEALEKRDTELAERLVRQHSLDLAAHVEKHCDFLD"
     gene            6197..7933
                     /gene="oxc"
                     /locus_tag="VHT22_01645"
     CDS             6197..7933
                     /gene="oxc"
                     /locus_tag="VHT22_01645"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HEX3136283.1"
                     /translation="MGSDTGIVPGGQETDGFQLVIEALKMNGIETIFGLPGIPITDLT
                     RRMQAAGLRVISFRHEQNAGYAASIAGFMMQKPGICLTVSAPGFLNGLAALANATTNC
                     FPMILVSGSSEREIVDLQQGDYEEMDQLAIAKPHAKAAFRVLHAEDIGVGVARAIRAA
                     VSGRPGGVYLDLPAKLFPQTMEAEAGKASLIKVVDPAPRQIPAPDAVRRALELLKSAK
                     RPLIILGKGAAYAQADADIRALVEKTGIPYLPMSMAKGLLPDTHEQSASAARSYVLPE
                     ADVVMLVGARLNWLLSQGKGKTWGGDNAKAWGGQKFVQIDISPQEADSNVRIDAPVVG
                     DIGSCVAAMLAGIGANWPKPPSDWLDAIAERKTKNVAKMSEALAKNPSPMNFHSALNV
                     VRDIVKANPDAILVNEGANALDFTRSIVDMYKPRKRLDVGTWGVMGIGMGFSVAAAVV
                     SGEQVIAVEGDSAFGFSGMECETICRYDLPVCVVILNNNGVYRGDEVNPTGGRDPSPL
                     VFVKGARYEMLMEAFGGVGVMVKTPAELRSAMEQAIRSRRPTLINAVIDEKAGTESGR
                     ITSLNPTAKKKP"
     gene            7944..>9025
                     /gene="frc"
                     /locus_tag="VHT22_01650"
     CDS             7944..>9025
                     /gene="frc"
                     /locus_tag="VHT22_01650"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HEX3136284.1"
                     /translation="MGKALDGVRILDFTHVQSGPTCTQLLAWMGADVIKVERAGEGDI
                     TRGQLRDIPGVDSLYFTMLNHNKRSITIDAKHPQGKELIEALVRKCDVLVENFAPGAL
                     DRMGFTWERIQELNPAMIVASVKGFGPGPYEDCKVYENVAQCAGGSASTTGFDDGPPL
                     VTGAQIGDSGTGLHLALGIVAALYQRKTTGRGQRVLAAMQDGVLNLCRVKLRDQQRLD
                     RTHVMEEYPQYPNGKFGDAVPRAGNASGGGQPGWILKCKGWETDPNAYIYFITQAPVW
                     KDICEVIGREEWLTDPRYATPKARLPHLMEIFGQIEEWTKTKTKFEAMDILNEHDIPC
                     GQRFGRRPARLDSEMQGLGNGSERVHL"
BASE COUNT         1569 a         2984 c         2912 g         1560 t
ORIGIN      
        1 catccgacag cacgatgacg ccgttgctct cgatcttggc ccggatgtcc ttcatgaact
       61 tggagttgcc caaccgcgca gcccgctcgt gattgcgcac cagccccggc atcagcgtgg
      121 cgccgaattc gcgcaccttg ccgctggcgt agtcgagcgg aaacgacgag atgtcgagct
      181 ggccgttgac catcgcgttc cattgctcgt tcggcttgaa cagcgacgcg ccgggataga
      241 cctgcacgtc gaggtcgacg ccggccgctt tggcatcgcg cgcaatgatc tgcaccattt
      301 cgtcgcgaac atcgcccttg ccaccgggga attgatgcga agcgcgcagc gtgaccggcg
      361 ccgccgccac cgagcctgcc actacgaccg ccgccagcgc ggtcagcacg cccaaatgcg
      421 tgaacttgct catgctgatt cctcctaaat gcatctttcg aaatacggac caagccggcg
      481 acggctctgt ggccgttcgc acttccagtc gcgctgtcgc acttcagccg tcgtagccga
      541 caagctcggc ggtcgcgccg cccgcactta cacgaaccgc acagtacttg aactccggaa
      601 tcttgccaaa tggatccagc accggattgg tcaacagatt cgccgcggcc tcgtaataac
      661 agaacgggat gaacaccgcg ccgcgtggcg tgccttcatc cgcgcgcgca gagagggcga
      721 tgctgcctcg tcgggaagcg acggtgatcg tgccgccggg atcgacgccg agcttcgcca
      781 ggtcgtcggg gttgatcgac gcgaccggcg aaggctcgat cgtgtcgagc accctcgccc
      841 gccgcgtcat gctgccggtg tgccagtgct cgagctggcg cccggtgatc agcacgaatg
      901 gatactcgcc gtccggccgc tcggctgcgg ggatgatcga ggccggcaca aagcggccct
      961 tgccattcgc cgtcgggaaa tcctcggtaa atacaacgcg ctgccccgga tcgccctcgt
     1021 gctcgcacgg ataggtgact gcgtgctccc gctcgagccg gtcccaggtg ataccggcga
     1081 tcgacggcat cgtccgtcgc atctcctcga acacttggct cggatgctcg taatgccagt
     1141 cgagtccgag cgtcctggcc atcgcgacga tgatcgccag atcctgtcgc gcctcgccgg
     1201 ggggttgcag tgcctgccgc ccgagctgca cgctgcggtc ggtattggtg aatgttccgg
     1261 tcttctcggg aaacgccgat gccggcagga tcacgtcggc cagatacccg gtctcggtca
     1321 ggaagatgtc ctgcacgacc agcatctcga gctcggccag cgcttgccgc gcgtggctga
     1381 cgtcgggatc ggacatcgcc gggttctcgc ccatgatgta catgccgcga atctgcccgg
     1441 cgtgcacggc gtccatgatt tcgacgacgg tgaggccggg ctgcggatcg agcgtcgttc
     1501 cccatacctg ttcgaacctg ctgcgcgttc cggcgttgtc gacgcgctgg tagtccggat
     1561 acatcatcgg gatcaatccg acgtcgctcg cgccctgcac gttgttctgt ccgcgcagcg
     1621 gatgcagccc ggtcccggga cggccgacct ggccggtcat catcgacagc gcgatcaggc
     1681 agcgggcgtt atcggtgccg tgcacgtgct gcgatatgcc catgccccag aggatcatcg
     1741 agccgcgcga tttggcgtac atcctcgcga cctcgcggat cgtcgctgca gggacgccgc
     1801 acaccggcgc catcgcctcg ggactgaagc cggcgacgtt gttgcggatt tcctcgaagc
     1861 cgctggtgcg gtcggcaacg aagctctgcg cgaccaggcc ttcctcgacg ataacgtgca
     1921 tcatcgcgtt cagcagcgcg acgtcggtgt ccggcttgaa ttgcagataa tgggtcgcgt
     1981 ggcgcgcgag ctcggagcgg cgcggatcgg cgaggatcag cctggtgccg ttcctgacgg
     2041 cgtttttgat ccaggtggcg gcgaccggat gattaaccac cggattggcg ccgatcagca
     2101 gcacgacatc ggccttcatc acgtccatca ccggattcga caccgcgccg gacccgatgc
     2161 cttcgagcag cgccgcgacg ctggacgcgt ggcaaaggcg cgtgcaatgg tcgacgttgt
     2221 tcgagccgaa tccggtgcgc accagcttct ggaacagata cgcttcctcg ttcgagccct
     2281 tggccgagcc gaagccggcg agcgccttgc caccgtgctc atcgcggatg cgcttgagcg
     2341 tgccacccgc cacctgcagt gcttcttccc agctcgcctc gcgaaatacc gcgagcggat
     2401 cagccgggtc catgacgaag tccgcggtct tgggcacacc gctcttccga atcagcggcc
     2461 gcgtcagccg ctgcgggtga tgcacatagt cgtagccata gcgccccttg acgcacaagc
     2521 gttcgtggtt tgccgggccg tcgcggcctt cgaccctaac gatctcgttg tccttgacgt
     2581 gatagtcgag ctggcaaccg acgccgcagt acgggcagac cgatgggacg actttgtcgg
     2641 cgacgaccag gtaggcttcg ttcgccggcg ccagcgcccc ggtggggcag gcttgcacgc
     2701 attcgccgca ggccacgcag gtcgacgctc ccatcgggtc gccaagatcg aacacgatcg
     2761 ccgagtgcgc gccgcggaac gcatagccga tgacgtcgtt gacctgctct tcacggcacg
     2821 cgcgcacgca gcgcgtgcac tggatgcagg catcgagatt gaccgcgatc gccgggtgcg
     2881 acaagtcggc cttgggctgc gcgcgcgccg cgaagcgcgg cgcgcccagc cccaaccagc
     2941 gcttccattg ctcgagctcc gaatccagtt tgtagacccg caccggcacg tcggcggcca
     3001 gcatctcgac gatgattttc tgtgagtgaa ccgcgcgcgg actgtcgctg ctcacttcca
     3061 tgccggcggt cggcttgcgg cagcacgaag gtgccagcac tcgctcgccc ttgatctcga
     3121 ccatgcaggc gcggcagttg ccgtcggggc gcatacccgg cttgtagcaa aggtgcggga
     3181 tctcgacccc atgccgcttt gccgcctcga tgatggtttc atccgggcgc gccgacgcgg
     3241 cttcgccgtt taggcgaaac tcgatcagtt cggccgcgag ggcggcttcg actggagcgt
     3301 tcatgcctcg gcctccgcgc cgactcgcga atcgcaggca tctacgtgtc ggcctccaag
     3361 caatcgctcg gcgacggccc ttcgggtctt tactgctccc gcctcgttca tgccagctcc
     3421 tgcggaaaat acttgaccac gcatgcaatc ggattcggcg ccgcctggcc gagcccgcag
     3481 atcgatgcgt cggccatcgc ctgtgaaagc tcggccagca gcgcagtatc ccatttcggc
     3541 ttcgacagca gcccgaccgc tttttcggtt ccgacgcggc agggcgtgca ctgtccgcag
     3601 gattcgtcgg cgaaaaagcg catcaggttg agcgccgcgt cacgcgcacg gtcgtgctgc
     3661 gacagaacga tcaccgcggc cgagccgatg aagcagccgt gcggctgcaa ggtgtcgaaa
     3721 tccagcggaa cgtcggccag cgacgccggc aggataccgc ccgaggcgcc gcctggaagg
     3781 tagccgtaga attcgtgccc cggcagcatc ccgccgcagt attcggcgat cagttcgcgc
     3841 accgtgatgc cggccggcgc caggtgcacc cccggcttgg ccacgcggcc cgacaccgaa
     3901 aacgagcgca ggcccttgcg gccgttgcgc ccctgaccgc caaaccacgc gccgcccttt
     3961 tcgaggatgt cgcggatcca gtacagcgtt tccatattgt gttcgagcgt cggccggccg
     4021 aaaagtccca cctgcgcgac atacggcggg cgcagacgcg gcatgccgcg cttgccctcg
     4081 atcgattcga tcatcgccga ctcttcgccg cagatgtagg cgccggcgcc gcgccgaagg
     4141 tgcaggctcg gcagcgggca cggagggtcg gcttcgagcg ccgagatctc gcgctcgagc
     4201 accgcgcgac atccggcgta ttcgtcgcgc agatagatat agacatcggc gatgccgacc
     4261 gcccacgccg cgatcagcac gccttcgagg aaccgatgcg gatctcgctc gagatagtag
     4321 cgatccttga acgtacccgg ctcgccttcg tcgatattga ccgccatcag ccgcggcgct
     4381 ggctctgcgc gtacgatttt ccatttcctg ccggccggaa agccggcgcc gcctaagccc
     4441 cgcaagccgg actcgtccag cgccgcggtc accgcttcga ccgtgcgttc gccgccgacg
     4501 caggcggcaa tcgtccggta gccgcccccg gcgcggtatg cggcaaggtc gatatacggc
     4561 ggcggctccg cctcgatccg cttgtctgcg atcgctgccg cgattttttc cggcgtcgct
     4621 tcgtcgaccg gatggcgccc gacgaccgcg gctggcgcgt gctcgcagcg tccgatgcag
     4681 ggcgcgccga cgacgcgtat gccggaagcg tgcgcccgct gaagggcttc gagcagagtg
     4741 tcggcgcccg ccatttggca cgaaagcgtc tcgcagacgc gaacggtcag cggcggcggc
     4801 ggcgcctcac cgtcggcgac gacgtcgaag tggtggtaga aggtcgccac ctcgtacacc
     4861 tcggtccgcg ccagcttcat ctcctcggcc agcgcgacga tgtgcccacc ggaaatatgg
     4921 ccgtagcggt cctggatccg gtgcaggaac tcgatcagga ggtcgcgcct gcggggcgcc
     4981 tcgcccagca gcacgcggat ttcggccagc gcctgcggct cgaccggccg acccttcgga
     5041 tgcgcgcgcg cgcggcgctt gcgatcgagc ccggaaatgt cgacgatcgc atgctcgttc
     5101 atcggcggtc ggccccgtcg gtgcaggttg gagccatcgt catggcgtca atcgagaaaa
     5161 tcgcaatgct tttcgacgtg cgccgcgagg tcgagcgagt gctggcgcac gaggcgctcg
     5221 gcgagctcgg tgtcgcgctt ttccagtgcc tcgatgatcc gcatgtggtc gatgatcgag
     5281 cgcgaggccc ggtcgctctg cgatatggtc atgcggcgta tcgcccgcac gtgcaggaac
     5341 aggtcgtcga tcgtcttccc catcagatgc gatcccgaca gtcggatgat cgcctggtgg
     5401 aacgcgatgt tggcgtccga gtattcgttg atgtgctcgg ccggcgtcga gttccggaat
     5461 tcgtcgaaca tgtgccgcag cgcgccgata tcctggtccg aggcattcag cgtcgccagt
     5521 cgcgccgcca tgctttccag ggccgcccac atctcgatca tttcgatgat ctgcttcttc
     5581 gtcttgcgca cgatgaatac gccgcgccgg gacaccgtgc gcagaaatcc ttcctgctcg
     5641 agcaaggtca tggcctcgcg gatcggcgtg cggctgactc caagcgcttg cgacagctgc
     5701 cgctcgtcca gccgcagctc ttcgcgatgc gcatagatgt cggcgtccat gatcgcctgc
     5761 ttgatcgccg agtacgcctg atcgcggaag cttacgttgg tcgtgatcgg ctgcagggcc
     5821 aaggtgcgcg ctgccgcctg cggatgcgcg ggcgatcgcg ttcgaggctg tctcggcttg
     5881 gtgggtgcgt cgatgtgtgt tgccatggtc atttcccatt ccatcgcaag ccagcctggc
     5941 cgactggttg ccgatcgcgg gaatcgctta tggtcatcaa gccaatagaa tccgtcactt
     6001 gcgtggtcca cgggattccg aacgcgttcg gccgactcgc tgaatcgcga ttgactttcg
     6061 ctgatatact gcatacgtta tgccgaatac ctaaaaaaag ccagcctgtc cgaccccgga
     6121 gcccgccatg ttagacacaa ccgcgaagcc tgcagcaaag ccggcggcca cgcccgccga
     6181 ggaaacgtcg aagacgatgg gcagcgatac cggtatcgtg cccggcggcc aggagaccga
     6241 cggctttcag ctggtgatcg aggccctcaa gatgaacggc attgagacga tctttggtct
     6301 tcccggcata ccgatcaccg acctcacgcg caggatgcag gcggcgggct tgcgcgtcat
     6361 ctccttccgt catgagcaga atgccgggta tgccgcttcc atcgccggct tcatgatgca
     6421 gaagccgggc atctgcctga cggtgtcggc ccccggcttt ctcaacggac tggcggcgct
     6481 cgccaacgcc acgaccaact gcttcccgat gatcctcgtc agcggctcga gcgagcgcga
     6541 gatcgtcgat ctgcagcagg gcgactacga ggagatggac caactcgcga tcgccaagcc
     6601 gcacgcgaag gccgcgtttc gagtcctgca cgccgaggac atcggcgtgg gcgtcgcgcg
     6661 ggcgatccgc gcggctgtct cgggccgtcc gggcggcgtc tatctcgacc tgccagccaa
     6721 gctgtttccg cagacgatgg aggcggaagc ggggaaggca tcgctgatca aggtcgtcga
     6781 tccggccccg cgccagatcc cggcgcccga cgccgtcagg cgcgcgctcg aactgctgaa
     6841 gagcgccaag cgtccgctga tcattctcgg caagggcgcg gcgtacgcgc aggccgatgc
     6901 ggacattcgc gcgctggtgg agaagaccgg catcccatac ctgccgatgt cgatggccaa
     6961 agggctgcta cccgacacgc acgagcaatc cgcatcggca gcgcgttcct atgtgctgcc
     7021 agaagccgat gtcgtgatgc tggtcggggc ccggctcaac tggctgctgt cgcagggcaa
     7081 gggcaagacg tggggcggcg acaacgccaa ggcgtggggc ggccagaagt tcgtgcagat
     7141 cgacatttcg ccgcaggagg cggacagcaa cgttcgcatc gacgcgccgg tcgtcggcga
     7201 catcggatcg tgcgttgcgg ccatgctcgc cggcatcgga gccaactggc cgaagccacc
     7261 ctccgactgg ctcgacgcca tcgcagagcg caagaccaag aacgtggcga agatgagcga
     7321 agcgctggcg aagaatcctt cgccgatgaa cttccacagc gcgctcaacg tcgtgcgcga
     7381 catcgtcaag gcgaatccgg acgccatcct cgtcaacgag ggcgcgaacg cgctcgactt
     7441 cacgcgcagc atcgtcgaca tgtacaagcc gcgcaaacgc ctggacgtcg gcacctgggg
     7501 cgtgatgggg atcggcatgg gcttttcagt cgctgcagcc gtcgtaagcg gcgagcaggt
     7561 gatcgcggtc gagggcgaca gcgccttcgg tttctccggc atggaatgcg agacgatctg
     7621 ccgctacgac cttccggtct gcgtcgtgat cctgaacaac aacggcgtct accgtggcga
     7681 cgaggtgaat ccgaccggag gacgcgatcc gtcgccgctg gtgttcgtca agggcgcgcg
     7741 ctacgagatg ctgatggagg ccttcggcgg cgtcggtgtc atggtcaaga cgccggccga
     7801 actgcgcagc gcgatggaac aagcgatccg ctcgcgcagg ccgacgctga tcaacgccgt
     7861 catcgacgaa aaggcgggca cggagagcgg ccgcatcacc agtctcaatc caacggccaa
     7921 gaagaaaccg taaggggagt gtaatgggca aggcactgga cggagtccgc atcctcgatt
     7981 tcacccacgt gcaatcgggc cccacctgca cgcagcttct cgcgtggatg ggcgccgacg
     8041 tcatcaaggt cgagcgggcc ggcgaaggcg acatcacccg cgggcagctg cgcgacatcc
     8101 ccggcgtcga cagcctgtac ttcacgatgc tgaaccacaa caagcgctcg atcacgatcg
     8161 acgccaagca tcctcaaggc aaggaactga tcgaggcgct ggtgcgcaaa tgcgacgtgc
     8221 tggtcgagaa tttcgcgccc ggcgcgctcg accgcatggg cttcacctgg gagcgaatcc
     8281 aggagctcaa tccggcgatg atcgtcgcgt cggtcaaggg cttcggccct ggaccgtacg
     8341 aagattgcaa ggtgtatgaa aacgtcgcgc aatgcgcggg agggtcggcg tctactaccg
     8401 gcttcgacga cggtccgccg ctggtgaccg gcgcgcagat cggcgactcg ggcaccggct
     8461 tgcatctggc gctcggcatc gtcgccgcac tttatcaacg caagaccacc ggacgaggcc
     8521 agcgcgtcct tgcggcgatg caggacggcg tgctcaacct gtgccgggtg aagcttcgcg
     8581 accagcagag gctggatcgc actcacgtca tggaggaata cccgcaatat ccgaacggca
     8641 agttcggcga cgcggttccc cgtgcgggca acgcttcggg cggcggccag cccggctgga
     8701 ttctgaaatg caagggctgg gaaacggatc cgaacgcgta catctacttc atcacgcagg
     8761 cgccagtgtg gaaagacatc tgcgaggtga tcggccgcga ggaatggctg accgatccac
     8821 gctacgcgac ccccaaggcg cggctgccgc acctgatgga gattttcggc cagatcgagg
     8881 agtggaccaa gaccaagacc aagttcgagg cgatggacat cctcaacgag cacgacatcc
     8941 cgtgcgggca acgcttcggg cggcggccag cccggctgga ttctgaaatg caagggctgg
     9001 gaaacggatc cgaacgcgta catct
//
LOCUS       DATANS010000112         4631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_676895, whole genome shotgun sequence.
ACCESSION   DATANS010000112 DATANS010000000
VERSION     DATANS010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676895"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /gene="pap"
                     /locus_tag="VHT22_01655"
     CDS             <1..1225
                     /gene="pap"
                     /locus_tag="VHT22_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HEX3136285.1"
                     /translation="WQALPPSGRLGIFMHAWYNDSMRARMQGRIDDDRLETHLEAIRE
                     HEQMLSDEGLVLLKFWIHLSKAAQKKRLKAIERDPRLSWRATRDDWKAYHAYRKSYPL
                     WETLLRETSTTAAPWYVVEGTDERYRNLTVGRILLDAMRATAKPSAAARANAVRHPVT
                     PIAPSAIDNVKLIRDLDLSKKLSARAYPAHLEKWQGALARLSRHKRFAERSLVVAFEG
                     VDAAGKGGAIRRVTGALDARQYVTVPIAAPTDEELAHPYLWRFWRKIPRRGGITLFDR
                     SWYGRVLVERVEHLSAPGEWMRAYAEINQFEEQLTQGGVIVVKFWLQLSKAEQLRRFR
                     AREHTPFKRFKITRDDWRNRKRWNDYEIAVCDMVDRTSTMLAPWTLVEAEDKRYARVK
                     VLKTIVHRIEAALER"
     gene            1222..2187
                     /locus_tag="VHT22_01660"
     CDS             1222..2187
                     /locus_tag="VHT22_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX3136286.1"
                     /translation="MSRDYSAPRWLAGAHAQTIYPALFGPPKIALRRERVDTPDGDFV
                     DFDWQDTSGAAPGTPTVVLFHGLEGSSSSHYARALMRRLQAIGWRGVVPHFRGCSGEP
                     NRLPRAYHSGDYAEIGWMLAMVKARLPAAPLYAVGVSLGGSALLNWLGREQARARSTL
                     VAAAAVSAPLDLAAAGTAIGQGFNRIYAQHFLSTLVPKALAMARRFPGSLDTAAVRRA
                     RSMHAFDDVVTAPLHGFDGVNDYWRRASSKPWLGGIAVPTLVLNAKNDPFIPASSLPG
                     PESVSRSVTLEQPAHGGHAGFAMSPFPGSLEWVPSRLLQFFAPMQ"
     gene            2245..3618
                     /locus_tag="VHT22_01665"
     CDS             2245..3618
                     /locus_tag="VHT22_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX3136287.1"
                     /translation="MNLPNDIFKAYDIRGIVGKTLTAPIVQAVGEALGTLAQERGRDT
                     LVIGRDGRLSGPELAGALSTGIRASGANVIDIGMVTTPISYFAAHHLKTQCSVMVTGS
                     HNPPDYNGLKMVIAGDTLAGDEIKALRTRIEAGELKRGAGSLRNADVAPAYFDRIAGD
                     VRLARPMKIAVDCGNGVAGAYAPTLFRRLGCEVTELFCEVDGRFPNHHPDPSQPENLR
                     DLVANLASTDNELGIAFDGDGDRLGVVTKDGRTIYADRQLMLFAADMLKRRPGATVIY
                     DVKSTRNLKPWIEQHGGKALLWKTGHSLIKAKMKETGAELAGEMSGHTFFRERWYGFD
                     DGMYAGARLLEILSHDADASAALHAIPDSLSTPELNIRCSDASPHALIDKLAATATFP
                     GAVDVIRIDGLRVEYPDGFGLARASNTTPVIVLRFEADDAAALARIQGEFRRVLSLAM
                     PGRPLPF"
     gene            3625..4041
                     /locus_tag="VHT22_01670"
     CDS             3625..4041
                     /locus_tag="VHT22_01670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016863249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3136288.1"
                     /translation="MTIDYSTSLEGVDWAALIASLAADRFDNGRTARQMEASFRNSYR
                     CVIARDGATIVGTGRALSDGVCNAYIVDVWTQSAYRRRGIGRRVVDMLCQALPGQHVY
                     LFTDDRRDFYAACGFVPRAVGMQRVVGEWLKNDSER"
     gene            complement(4095..4520)
                     /locus_tag="VHT22_01675"
     CDS             complement(4095..4520)
                     /locus_tag="VHT22_01675"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HEX3136289.1"
                     /translation="MRRVDRLFENYGLYHQDPINKAIHWICVPLIVWSVLGMVWAASP
                     LAACAAIAAMILFYLFLSVPLAFGMLAVVAAMAWALTLLGDRLLMVSLVTFAAAWVGQ
                     FIGHAIEGRKPAFVDDLRSFLVAPAWLLGDLYRRLGLRF"
BASE COUNT          828 a         1560 c         1503 g          740 t
ORIGIN      
        1 ttggcaggcg ttgccgccga gcggccggct cggcatattc atgcacgcct ggtacaacga
       61 ctcgatgcgt gcgcggatgc agggccgcat cgacgacgat cgactcgaga cgcatctgga
      121 ggcgatccgc gagcacgagc agatgttgag cgatgaagga ctcgtgctgc tcaagttctg
      181 gatccacctg tccaaagcgg cgcagaaaaa acgcctgaag gcgatcgagc gcgacccgcg
      241 cctttcctgg cgcgcgaccc gcgacgactg gaaggcgtac cacgcctatc gcaaatccta
      301 tccgctgtgg gagacgctgc tgcgcgagac gtcgaccacg gcggcgccct ggtatgtggt
      361 cgaggggacc gacgagcgtt accgcaacct gaccgtcggg cgcatcctgc tcgacgcgat
      421 gcgcgcgacg gcgaagccgt cggcagcggc gcgcgcgaat gcggtcaggc acccggtgac
      481 gccgatcgcg ccttcggcga tcgacaacgt caagctgatt cgcgatctcg acctgtcgaa
      541 gaagctctcc gcgcgagcct atccggcgca cctcgagaaa tggcagggtg cgcttgcccg
      601 gttgtcccgg cacaagcgtt tcgccgagcg ttcgctggtc gtcgcgttcg aaggcgtcga
      661 tgccgccggc aagggcggcg cgatacgccg cgtcaccggc gcgctcgatg cgcgccaata
      721 cgtgacggtc ccgatcgcgg cgccgaccga cgaggagctg gcacatccgt atctgtggcg
      781 cttctggcga aagattccgc ggcgcggtgg cataacgctg ttcgatagat cctggtacgg
      841 gcgcgtactg gtcgagcgcg tcgagcacct aagcgcgccc ggcgaatgga tgcgcgcgta
      901 tgccgagatc aaccagttcg aggagcaatt gacgcaaggc ggcgtcatcg ttgtcaaatt
      961 ctggctgcag ctcagcaagg ccgaacagct gaggcgcttc cgcgcgcgcg agcacacgcc
     1021 gttcaagcgc ttcaagatca cgcgtgatga ctggcgcaat cgcaaacgct ggaacgacta
     1081 cgagatcgcg gtatgcgaca tggtcgatcg cacgagcacg atgctcgcgc cgtggacgct
     1141 ggtcgaagcc gaggacaagc gctacgcgcg cgtcaaggtc ctcaagacga tcgtgcaccg
     1201 catcgaggcg gcactcgagc gatgagccgc gactattcgg cgccgcgatg gcttgccggc
     1261 gcgcacgcgc aaactatcta tcccgcgctg ttcgggccgc cgaagatcgc cttacgccgt
     1321 gagcgcgtcg atacgccgga cggcgacttc gtcgacttcg actggcagga cacgagcggc
     1381 gccgcaccgg gcacgccgac ggtggtgctg tttcacggac tcgaaggcag ctcgtcgtcg
     1441 cactatgcgc gcgcgctgat gcggcgcttg caggcgatcg gctggcgcgg cgtcgtgcca
     1501 catttccgcg gttgcagcgg cgagccgaac cgtttgcccc gcgcctatca ttcgggcgac
     1561 tacgccgaaa tcggctggat gctcgcgatg gtcaaggcgc gcctgccggc ggcaccgctg
     1621 tacgcggtcg gcgtttcgct gggcggcagc gcgctgctga actggctcgg gcgcgagcag
     1681 gcgcgcgcgc gctcgacgct ggtcgcggcg gcagccgtgt ccgcgccgct cgacctggcc
     1741 gcggccggca cggcgatcgg ccagggcttc aaccgtattt acgcgcagca cttcctgtcg
     1801 acgctggtgc cgaaggcgct cgccatggca cggcgcttcc cgggctcgct cgacaccgca
     1861 gccgtccgcc gcgcgagatc gatgcacgcg ttcgacgacg tcgtcaccgc cccgctgcat
     1921 ggcttcgacg gcgtcaacga ttattggcgg cgcgccagca gcaagccctg gctcggcggc
     1981 attgccgtgc cgacactggt gctcaacgca aaaaacgatc cattcattcc ggcaagctcg
     2041 cttcccggcc ccgagtcagt cagccgctcc gtcacgctgg agcagcctgc gcacggcggc
     2101 cacgccggct tcgcaatgtc tccgtttccc ggtagtctgg agtgggtccc gagccgcctg
     2161 ctacaattct tcgcgccaat gcaatagccg caggccgctc gcccgcgggg caagccgtgc
     2221 catgtcgatc cactcgattt cccgatgaac cttcccaacg atatcttcaa ggcctacgac
     2281 attcgcggca tcgtcggcaa gacgctgacc gcgcccattg tacaggcggt cggagaagcg
     2341 ctcggcacgc tggcgcagga gcgcggccgg gacacgctgg tcatcggccg cgacggccga
     2401 ctgtcgggcc cggagctcgc cggcgcgctt tcaaccggca tccgcgccag cggcgccaac
     2461 gtcatcgata tcggcatggt cacgacaccg atcagctact tcgccgcaca tcatctgaag
     2521 acgcagtgca gcgtgatggt gaccggaagc cacaatccgc ccgattacaa cgggctcaag
     2581 atggtgatag ccggcgacac gctcgcgggc gacgagatca aggccttgcg cacacgcatc
     2641 gaggccggcg agctgaagcg cggtgcaggc tcgctgcgca acgccgacgt ggcgccggct
     2701 tactttgacc ggatcgccgg tgacgtccgg ctggcccgac cgatgaaaat cgcggtcgat
     2761 tgcggcaacg gcgtcgcggg cgcctatgcg ccgacgctat ttcgccgcct cggctgcgag
     2821 gtcaccgagc ttttctgcga agtcgatggc cgctttccga accatcatcc ggatccatcg
     2881 caaccggaga accttcgtga cctcgtggcc aacctcgcct cgactgacaa cgagctgggc
     2941 atcgcattcg acggcgacgg cgaccgcctg ggcgtagtca ccaaggacgg gcgcacgatc
     3001 tacgccgacc ggcagctgat gctgttcgcc gccgacatgc tgaagcgccg tcccggcgcg
     3061 acggtcatct acgacgtcaa gtcgacgcgc aatctcaagc catggatcga gcagcatggt
     3121 ggcaaggcgc tgctgtggaa aaccggacat tcgctgatca aggccaagat gaaggaaacc
     3181 ggcgcggaac ttgccggcga aatgagcggg catacgttct tccgggaacg ctggtacgga
     3241 ttcgacgacg gcatgtacgc cggcgcgcgc ctgctcgaaa ttctctccca cgatgccgac
     3301 gcctcggccg cgctgcatgc gattcccgac agcctcagca cgccggagct caatatccgc
     3361 tgttccgatg cctcgccgca tgcgctgatc gacaagctcg ccgcgacggc gacctttccg
     3421 ggcgccgtcg acgtcattcg catcgacggc ctgcgtgtcg agtatcccga cggtttcggg
     3481 ctcgcgcgtg cgtcgaacac gacaccggtc atcgtcctgc gcttcgaggc ggatgacgcg
     3541 gccgcgctgg cgcgcatcca gggcgagttc cgccgcgtgc tgagcctggc gatgccgggc
     3601 aggccattgc cgttctagga cccgatgacg atcgactatt cgacttcgct cgaaggcgtc
     3661 gactgggcgg cattgatcgc ctcgcttgcc gccgatcggt tcgacaacgg acgcacggcg
     3721 cgacagatgg aagcctcgtt ccgcaacagc taccgctgcg tcatcgcccg cgacggcgcg
     3781 acgatcgtcg gtaccggacg cgcgctatcc gacggcgtgt gcaacgccta catcgtcgac
     3841 gtctggaccc agtccgcgta ccgacggcgc ggcattggcc ggcgcgtcgt cgacatgctc
     3901 tgtcaggccc tgcccggcca gcacgtctat ctgtttaccg acgatcggcg cgatttctat
     3961 gccgcctgcg gttttgtgcc acgagctgtc ggcatgcagc gcgtcgtcgg cgagtggctg
     4021 aaaaacgaca gcgagcgctg aacctccaga gcggaacgac gccgtacgcg tacagcggcg
     4081 cgccgatcgc ggggtcaaaa cctgagacca aggcgcctgt aaaggtcgcc gagcagccac
     4141 gccggcgcca ccagaaacga gcgcagatcg tcgacgaagg ccggcttcct gccttcgatc
     4201 gcgtggccga tgaactgccc gacccacgcc gcggcgaacg tgaccagcga taccatcagc
     4261 aagcgatcgc cgagcaaggt cagcgcccac gccatggcgg caacgacggc cagcatgccg
     4321 aacgcaagcg gcaccgacag aaacaggtaa aacaaaatca tggctgcgat tgccgcgcag
     4381 gccgccagcg gcgacgcggc ccacaccatg cccagcacgc tccagacgat cagcggcacg
     4441 catatccagt ggatcgcctt gttgatcgga tcctgatggt aaaggccgta gttttcgaac
     4501 aggcgatcga cgcgccgcat gctgcgccta tgttgtcatc ggatggtcga aatcctccca
     4561 tgcgcaagct tgcgggtcca gcccgggaga cactggccgc tgcgctataa tcgaagcgcg
     4621 ccgttcgcgg g
//
LOCUS       DATANS010000113         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_509966, whole genome shotgun sequence.
ACCESSION   DATANS010000113 DATANS010000000
VERSION     DATANS010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509966"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="VHT22_01680"
     CDS             <1..1174
                     /locus_tag="VHT22_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136290.1"
                     /translation="SITGNNPAPLKSATAAQKETQTPAKEAQKPAEPAPHESNDKTSD
                     SAAPQLLAAEFSPPQVQDGQDALLILAANDDLSGIRNISGSVTSPTGKALQGFAAQRD
                     GEMRYVARIQIPRDAEEGTWRINFISLSDNASNTANMTFAQSPVLQNALLRVQSSRPD
                     STPPSVKRVWLERPSMTAGEKNTIFVHAIDDKSGVQLVTGVMLSPTGLARLGFGCHIT
                     ENDIWACELVTPKKLDCGDWKLEQLQLQDKANNMGTERGPIVTAVQLSIMSEACDSTA
                     PDMRAFTLDPTVIGNVEQGTIHVTAIVNDDNSGVASISGQAAGPAQAGQQPPRLFFSL
                     RPTGDNQTWVGDIVVPKLAAKGIWSIIWVQVLDDARNLKSFSRNDPVLADAAFTVR"
     gene            complement(1183..1854)
                     /locus_tag="VHT22_01685"
     CDS             complement(1183..1854)
                     /locus_tag="VHT22_01685"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX3136291.1"
                     /translation="MTTRVYLARHGATELSAEDRFAGAVDVLLSDAGRDQARLLGDRL
                     ATEELAAVYASPMKRTIETAQLASAAQSCDVTPVDGLREIAHGRWEGMLRAEVEQQFP
                     QEYTRYESDPFSFAPLGGETGLQVIARALPALLEIVERHTNERILIVSHKATIRLLLS
                     SLLGFDARKYRDRLDQSPAALNILDFKNLANARLTLFNDTSHYTGAIPAMPGAHLSKV
                     WDERK"
BASE COUNT          468 a          732 c          781 g          402 t
ORIGIN      
        1 ctcgatcacc ggaaacaatc ccgcgccgct gaagtccgcg acggcggcgc agaaggaaac
       61 gcagacgcct gcgaaagagg cccagaagcc ggcggagcct gcgccgcacg aatcgaacga
      121 caagacatcc gactccgcgg cgccacagct tctcgccgcc gaattctcgc cgccgcaggt
      181 gcaggacggt caggatgcgc tgctcatcct cgctgcgaat gacgatctct ccggcattcg
      241 caacatctcg ggcagcgtca ccagcccgac cgggaaagcg ctgcagggat tcgccgcgca
      301 gcgcgacggc gagatgcgtt acgtcgcacg cattcagatt ccgcgcgatg ccgaagaagg
      361 gacgtggcgg atcaacttca tcagcctgag cgacaacgcg agcaacaccg cgaacatgac
      421 gttcgcgcaa tcgccggtgc tgcagaacgc gctgctgcgc gtgcagtcgt cgcgtcccga
      481 ttcaacgccg ccgtcggtga agcgcgtgtg gctggagcga ccgtcgatga cggcggggga
      541 gaagaacacg atcttcgtgc acgcgatcga cgacaagtcg ggcgtgcagc tcgtgaccgg
      601 cgtgatgctc agcccgaccg gactcgcgcg gctcggcttc ggttgtcata tcaccgagaa
      661 cgacatctgg gcgtgcgagc tcgtgacgcc gaagaagctc gactgcggcg actggaagct
      721 cgagcagctc caactgcagg acaaagcgaa caacatgggc accgagcgcg gcccgatcgt
      781 gaccgcggtg cagctgtcga tcatgagcga agcgtgcgac agcacggcgc cggacatgcg
      841 cgcgttcacg ctcgatccga ccgtgatcgg gaacgttgag cagggaacga ttcacgtcac
      901 ggcgatcgtg aacgacgaca actcgggagt cgcgagcatc agcggtcagg ccgcggggcc
      961 ggcgcaggcg ggacagcagc cgccgcgttt gttcttctcg ctgcggccga cgggcgacaa
     1021 tcagacgtgg gttggcgaca tcgtggtgcc gaagctcgcg gcgaagggaa tctggagcat
     1081 catctgggtg caggtgctcg atgacgcgcg gaacctgaag tcgttttcgc gaaacgatcc
     1141 ggtgctagcg gacgcagcgt tcacggtgcg ctgagcgggg cgctacttgc gctcgtccca
     1201 caccttcgag agatgcgcgc ccggcatcgc gggaatcgcg ccggtgtagt gcgacgtgtc
     1261 gttgaacagc gtcagccgtg cgttcgcgag gtttttgaag tcgaggatgt tcagcgcggc
     1321 cggactttga tcgaggcggt cgcgatattt gcgcgcgtcg aatcctagca gtgagctgag
     1381 cagcagccgg atcgttgctt tgtgcgagac gatgaggatg cgctcgttcg tatgccgctc
     1441 gacgatctcg agcagcgcgg ggagagcgcg cgcgatgacc tgcaatccgg tttcgccgcc
     1501 gagcggcgcg aacgagaagg gatcgctttc gtatcgcgtg tactcctgcg gaaactgctg
     1561 ctcgacttcc gcgcggagca tgccttccca gcgaccgtgc gcgatctcgc gcagtccgtc
     1621 gaccggcgtc acatcacacg attgcgcggc ggatgcgagc tgtgccgtct cgatggtgcg
     1681 tttcatcgga ctggcgtaga cggctgcgag ctcttcggtg gcgaggcgat cgccgaggag
     1741 gcgagcctgg tcgcgtccgg catcggacag caacacatcc accgcccccg caaagcggtc
     1801 ctcggcggaa agctcggtcg caccgtgtcg tgcgagatat acgcgcgtcg tcaaaaatta
     1861 aaaggctcgc gatctctgat ctcgcgagcc cattccccgg tccgttaaca gccaccagag
     1921 ggcattcgaa ccgctgtgcg ccgcaacacc ctttgccgtt gcgacacttg cgcgacacgt
     1981 tgacagctgc aaagtagcat ccgtgtcgcc gcgccggctt ccatcgcgct ccggtgcgat
     2041 cctcgagatc gttgacagct gcgtggagca tccgatccga ggcagatttc aatctagttc
     2101 cggcgcctgg gaagggcaat acgtcgccgc ggtttttttt cgcggcggct caatgtgaaa
     2161 cgagctcatt cgctgcggaa gaaatccgac gctgcgggag ttggcagaga tccgcgccgc
     2221 gtgtaagata cgcagcccaa ccgatctcac gccgagatcc aatcacgcga aaggttttaa
     2281 aacacatccc atgacccagt cgagagaagc cgccgaggcg aatagtttcg ccgaggcgtt
     2341 tgaagcgagc ctgaatttca aaactcccga gcagggtgaa ctg
//
LOCUS       DATANS010000114         1634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_215473, whole genome shotgun sequence.
ACCESSION   DATANS010000114 DATANS010000000
VERSION     DATANS010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215473"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..>1634)
                     /locus_tag="VHT22_01690"
     CDS             complement(178..>1634)
                     /locus_tag="VHT22_01690"
                     /inference="COORDINATES: protein motif:HMM:NF006179.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HEX3136292.1"
                     /translation="IVSAAAHAGYPVQSTSIPGVAQRTGATTYYIEILPVQASELEGK
                     RPVLALTPGVGDIDVAVASELLEAGRTVANGFVTPDRTHVIASLSRFYAMDEKIAMAD
                     GRFDQDRLIKVIRDFSKDALLVDMDALAKQSGSIINAVMLGAIAGCNRLPIKPEQFEA
                     AIREDGKAVDSNLRGFRAGFDAARANLAPASPADKKKRAAATPAMVEHEVARTFPAVA
                     QTIGLEGVRRLIQYQGVPYAWLYLSRLRPIVEADQIAGAQGKLLKEVARHLAVRMSYE
                     DVVRVAQAKIAPTRMQRIARDELHVAGEPFSVHDFLKPGIEEMCQLLPPFLARPILRM
                     AERRGWLGRVYFGMEVNSTSVRGFLRFLMLAKLRRLRPYGYRYKQEQQQIENWLALIR
                     EAARKSPALALEVAECARLIKGYGDTHARGLANYRAIEERVIRPALAGQIAPARAVDA
                     LASARTAALVDPEGEGLAKCLADIDGPAMRIAAE"
BASE COUNT          287 a          521 c          533 g          293 t
ORIGIN      
        1 gccgtttagg cccgaaaagc cgtcgtttta ttggtgcaaa gtcttatccg acggggcgcg
       61 gggtgacaac ggatgacacc gcaatcctgt caggtcgctt agggaggcaa tgtcgtgccc
      121 gactaagcgg tatgcccggc acaaggccag gcatgacagg gaagagaaga ggtgacccta
      181 ctccgctgca atgcgcatcg ccggcccgtc gatgtcggcg aggcacttgg cgaggccttc
      241 gccctccggg tcgacaagcg ccgctgtgcg cgcgctggcg agcgcgtcaa ccgcgcgtgc
      301 tggtgcgatc tggcccgcca gcgcgggccg gatcacgcgt tcttcgatgg cgcggtaatt
      361 ggcaagcccg cgcgcgtgcg tatcgccata gcccttgatc agccgcgcgc attcggcgac
      421 ttcgagcgca agcgcgggtg acttccgcgc ggcttcgcgg atgagggcga gccagttttc
      481 gatctgttgc tgctcctgct tgtagcgata gccataggga cgaaggcgcc gcagttttgc
      541 gagcatcagg aaccgcagaa aacctcgaac ggatgtggag ttgacctcca tgccgaaata
      601 gacgcggccg agccagcctc tgcgttccgc catgcgcagg atcggtctcg cgaggaacgg
      661 cggcagtagc tggcacatct cctcgatacc gggcttgagg aaatcgtgca cggagaaagg
      721 ttcgccggcg acgtgcaact cgtcgcgcgc gatgcgctgc atgcgcgtcg gcgcgatctt
      781 cgcctgcgcg acccgaacga cgtcttcgta cgacatacgc acggcgaggt gccgcgcgac
      841 ttccttgaga agctttccct gcgcgccggc gatctggtcg gcctcgacga tcggccgcag
      901 gcggctgaga taaagccacg cgtagggaac gccctgatat tggatgagac ggcgcacgcc
      961 ttcgagcccg atcgtctggg cgaccgcagg aaatgtgcgc gcgacctcat gctcgaccat
     1021 ggccggcgtc gccgcagcgc gcttcttttt gtcggccggg cttgccggcg caagattcgc
     1081 gcgcgccgca tcaaagccgg cgcggaagcc gcgcaggttg ctgtccaccg ccttgccgtc
     1141 ttcgcggatc gcggcctcga actgctccgg ctttatcggc agccggttgc agccagcaat
     1201 cgcgccgagc atgaccgcat tgatgatcga accgctttgc ttcgcgagag catccatgtc
     1261 gacgagcaaa gcgtctttcg aaaaatccct gatgaccttg atcagtctgt cctggtcgaa
     1321 acgcccgtcg gccatggcga tcttctcatc catcgcgtag aagcggctga gcgatgcaat
     1381 gacatgcgtg cggtcgggcg taacgaagcc gttcgccacc gtgcggccgg cttccagcaa
     1441 ttcgctggcc accgcgacgt cgatatctcc gacaccgggc gtgagcgcga gcaccggacg
     1501 ctttccctcg agctcggacg cctgcaccgg caaaatctcg atgtaatagg tcgttgcgcc
     1561 ggtgcgttgc gcgacaccgg ggatcgacgt gctctgcacc ggatagccag cgtgcgcggc
     1621 ggccgacacg atcc
//
LOCUS       DATANS010000115         2415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_89919, whole genome shotgun sequence.
ACCESSION   DATANS010000115 DATANS010000000
VERSION     DATANS010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89919"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1509)
                     /locus_tag="VHT22_01695"
     CDS             complement(<1..1509)
                     /locus_tag="VHT22_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX3136293.1"
                     /translation="MRRFKSDILILGSGGAGLFAALHAHAADPSLSIAIAVKGLLGKC
                     GCTRMVQGGYNVALAAGDSVERHFMDTIEGGKWLPDQDLAWALVNGAVERVHELENEL
                     GCFFDRNPDGTVHQKAFAGQTFDRTVHKGDLTGIEIINRLSEQVWARGIARLEEHRAL
                     EFILTADGSALAAVLLVDMRTGEYVLAEAKAVLLATGGGPTMYRYHTPSGDKSCDGLA
                     MALAAGLPLRDMEMVQFHPTGLLAGPHTRMTGTVLEEGLRGAGGYLLDGRGVRFMQNV
                     DPRAERATRDIVSRAIDAEMRAGRTTPHGGVWLSMAHLGAENVRRQFKGMVERCADCG
                     FNLADRVEVVPTAHYMMGGVEFASDCTTALRGLFVAGEDSGGVHGANRLGGNGVANST
                     VFGAIAGDAMAAWVAGEGAFRDADSAAIQAALDRAETPFGRGEDADLDAIRERLFATM
                     WDDAGIVRDSLGLERAAITLATLADELGAYALPAGARERTFNLSWHDWLNLGS"
     gene            complement(1510..2133)
                     /locus_tag="VHT22_01700"
     CDS             complement(1510..2133)
                     /locus_tag="VHT22_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HEX3136294.1"
                     /translation="MAHHDLTTPITEAQTRALRIDDSVTLNGPLFGIRDATQIAMFDR
                     GRATRFDLAGHAVIHTAPNVKKVEASPAHPAGYAPLCIGTTTSSRMERFTRPLLERCG
                     VRLVIGKGGLGAGSLAAFGELGGAYLAIVGGTAALETTWIEAIEDVDLDDLNPESLWR
                     FRVRGFGPLRVAMDSHGGSLYTSVDADARSKRAAALATLGVNPRPAR"
     gene            complement(2137..>2415)
                     /locus_tag="VHT22_01705"
     CDS             complement(2137..>2415)
                     /locus_tag="VHT22_01705"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX3136295.1"
                     /translation="LCVHLAKIAATRPLGTHCADAEGAKLEDELSRAVNMLGVGPQGL
                     GGDATAFAVHVELAATHITMNPVAVNMQCHSARRASAAITTAGVSYGF"
BASE COUNT          385 a          848 c          805 g          377 t
ORIGIN      
        1 gctccccagg ttgagccagt catgccagct caggttgaaa gttcgttcgc gcgccccggc
       61 gggcagcgca taggcgccga gctcatcggc gagcgtggcc agcgtgatgg cggcgcgctc
      121 gaggccgagg gaatcgcgca cgatgcccgc gtcgtcccac atcgtcgcga acaaacgctc
      181 gcggatcgcg tccaggtcgg catcttcgcc gcggccgaac ggcgtctcgg cgcggtcgag
      241 cgccgcctgg atcgccgcgc tatccgcatc gcgaaacgcg ccttcgcccg cgacccacgc
      301 cgccatcgca tcgccggcga tcgcgccgaa caccgtcgaa ttggccacgc cgttgccgcc
      361 cagccggttg gcgccgtgaa caccacccga atcctcgccg gcgacgaaca atccgcggag
      421 cgccgtcgtg cagtcggaag cgaactcgac tccgcccatc atgtaatgcg cggtcggcac
      481 gacttcgacg cggtcggcga ggttgaagcc gcaatcggcg cagcgctcga ccatcccctt
      541 gaactggcgc cgcacatttt cggcgcccag atgtgccatc gacagccaga cgccgccgtg
      601 cggcgtcgtg cgcccggcgc gcatctcggc gtcgatcgcg cgcgagacga tatcgcgggt
      661 ggcgcgctcg gcgcgcggat cgacgttttg catgaagcgg acaccccgcc cgtcgagcag
      721 ataaccaccg gcaccgcgca ggccctcctc gagcacggtg ccggtcattc gcgtgtgcgg
      781 gccggcaagc agtccggtcg gatggaattg caccatctcc atgtcgcgca gcggcaggcc
      841 cgcggccagc gccatcgcca gcccgtcgca gctcttgtcg cccgaaggcg tgtggtaccg
      901 atacatcgtc gggccgccgc cggtagcgag cagcaccgcc ttcgcttcgg cgagcacgta
      961 ctcgccggtg cgcatgtcga ccagcagcac cgcagccagc gccgagccgt cggcggtaag
     1021 aatgaactcc agcgcgcgat gctcttccag ccgggcgatg ccgcgcgccc acacctgctc
     1081 ggacagccgg ttgatgatct cgatgccggt caggtcgcct ttgtgcactg tgcggtcgaa
     1141 tgtctgtccg gcaaacgcct tctggtgcac ggttccgtcg ggattgcggt cgaaaaagca
     1201 gccgagctcg ttctcgagct cgtgcacgcg ctcgaccgcg ccattgacca gtgcccaggc
     1261 gagatcctgg tcggggagcc atttgccgcc ttcgatcgtg tccatgaagt gtcgctcgac
     1321 cgaatcgccg gcggcgaggg ccacgttgta accgccttgc accatgcgcg tgcagccgca
     1381 tttgccgagc agacccttga cggcgatggc gatcgacagc gacggatcgg cggcgtgagc
     1441 gtgcagcgcc gcgaacaagc ccgcaccgcc cgagccgagg atcaggatgt cgctcttgaa
     1501 ccgccgcatt cagcgtgccg ggcgggggtt gacgccgagc gtcgcgagcg ctgccgcgcg
     1561 cttgctacgc gcatcggcat cgaccgacgt gtacaggctg ccgccgtgac tatccatcgc
     1621 cacgcgcagc gggccgaagc cacggacgcg aaatcgccac aagctctcgg gattgaggtc
     1681 gtcgagatcg acgtcctcga ttgcttcgat ccaggtcgtc tccagtgccg cggtgccgcc
     1741 gacgatcgcc aggtatgcgc cgccgagctc gccgaacgcc gcgagcgatc ccgcgccgag
     1801 cccgcctttg ccgatgacga ggcggacacc gcagcgctcg agcaacggac gggtgaagcg
     1861 ctccatccgg gaggaagtcg tcgtgccgat gcaaagcggt gcgtagccgg ccggatgcgc
     1921 gggcgacgcc tcgacttttt tcacgttggg cgccgtgtga atgaccgcat ggcccgcgag
     1981 atcgaagcgt gtcgcccgac cgcgatcgaa catcgcgatt tgcgtcgcgt cgcgaatgcc
     2041 gaacagcggc ccgttcaagg tgacggaatc gtcgattcgc agcgcgcgcg tttgcgcctc
     2101 ggtgatcggc gtcgtcagat cgtgatgcgc catgcgttag aagccgtaac tgacgccggc
     2161 agtggtaatc gccgcgctgg cccgccgcgc cgaatggcac tgcatgttca ccgcgaccgg
     2221 gttcatcgtg atatgcgtcg cggcgagctc gacgtgcacg gcgaacgccg tggcgtcgcc
     2281 gcccaggccc tgcggtccga cgccgagcat gttgaccgcg cgcgagagct cgtcctcgag
     2341 tttcgccccc tcggcatcgg cacaatgcgt gcccagcggc cgcgtcgcgg cgatcttggc
     2401 caggtgcacg caaag
//
LOCUS       DATANS010000116         1689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_468331, whole genome shotgun sequence.
ACCESSION   DATANS010000116 DATANS010000000
VERSION     DATANS010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468331"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VHT22_01710"
     CDS             <1..356
                     /locus_tag="VHT22_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136296.1"
                     /translation="GVFGVLSPHLIAGLGKLVIHQGLATIAIANFALLLIPTTLMGAT
                     LPMLVALLVRRQSTVGVSIGGLYCLNTFGAAAGALAVGFVCFYFFEIDTVIYAAAAIN
                     FLVSAVALLIVRRGE"
     gene            353..>1689
                     /locus_tag="VHT22_01715"
     CDS             353..>1689
                     /locus_tag="VHT22_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136297.1"
                     /translation="MMSLLPWFLSFCVGFLSLSVEILWVRVVSFKFHTMPHAFSFVLA
                     MYLVGIAVGAYVGKLCCKHERHLYLIGAVVLLLSGTFDIMTPRFAGDLLSGGFDNEGL
                     TSAALAIITTAAIKSVLFPIAHHLGSNAAGNVVGRSVSRIYSGNIAGSTLGPLLTGFV
                     LLDHFGVEQCFMMIGTAGLALAGVTALLGDQSRLRALPIALALVMLVLLRPWQTPDLV
                     AALSDKWWDNHRAPVGHVIQNKHGIISTSPDAKHGDIVFGGNVYDGRINVDMDVNSNG
                     LERPFLLGASHPAPRRVLVVGMSTGAWTRVITSFPHVERIDIVEINPGYLQLVARYPE
                     VAPLLSDPRVHIHIDDGRRWLRRHPEATFDLVVQNTTYHWRANATNLLSREYFAEVKS
                     HMRPGAIATFNTTGSLDVLRTAQEVFPAAFKFRWFVYVSDHDFRTPPDVAFAR"
BASE COUNT          251 a          565 c          525 g          348 t
ORIGIN      
        1 tcggtgtgtt cggcgtgttg agcccgcatc tcatcgccgg gctcggcaag ctcgtcattc
       61 atcagggcct ggcgacgatc gctatcgcca atttcgcact gctgctgatt cccacgaccc
      121 tgatgggcgc cacgctgccg atgctggtcg cgctcctggt caggcgccaa agcaccgtcg
      181 gcgtgtcgat cggtggactg tattgcctga acacgttcgg tgccgcggcc ggcgcgctcg
      241 ccgtcggctt cgtctgtttc tatttcttcg agatcgatac ggtcatctac gcggccgcgg
      301 ccatcaactt cctggtcagc gccgtggcgc tgctcatcgt gcggcgaggc gaatgatgag
      361 cctgctgccg tggttcttat cgttctgcgt cggcttcctc agcctgagcg tggagatcct
      421 gtgggtgcgc gtggtctcgt tcaagtttca cacgatgccg cacgcgtttt cgttcgtgct
      481 cgccatgtat ctcgtcggca tcgccgtcgg cgcgtacgtg ggcaagctct gctgcaagca
      541 cgagcggcac ctgtacctga tcggcgctgt cgtcctgctg ctcagcggca cgttcgacat
      601 catgacgccg cgcttcgccg gcgacctgct gtccggcgga ttcgacaacg agggcttgac
      661 ctcggcggcg ctcgccatca tcacgaccgc ggcgatcaag agcgtgctgt ttccgatcgc
      721 gcatcatctc ggctcgaacg cggcgggcaa cgtcgtcggt cgctcggtgt cgcgcatcta
      781 ctccggcaac atcgccggct cgacactcgg accgctgctt acgggcttcg tcctgctcga
      841 tcacttcggt gtcgagcagt gcttcatgat gatcggcacg gcggggctcg cgctcgccgg
      901 ggtcacggcg ctgctgggcg atcagagccg gttgcgcgcg ctgccgatcg cactcgcgct
      961 tgtcatgctg gtcctgctgc gtccgtggca gacacctgac ctggtcgccg cgctgtcgga
     1021 caagtggtgg gacaaccatc gcgctcccgt cgggcacgtg atccagaaca agcacggcat
     1081 catttcgacg tcgcccgatg cgaagcacgg cgacatcgtc ttcggcggca acgtttacga
     1141 cggccgcatc aacgtcgaca tggacgtgaa ctcgaacggc ctggaacgac cgttcctgct
     1201 gggggcttcg catcccgcgc cccgtcgcgt actggtcgtc ggcatgagca cgggcgcgtg
     1261 gacgcgcgtc atcacgagct tcccgcacgt cgagcgcatc gacatcgtcg agatcaatcc
     1321 cggttacctg caactggtcg ctcgatatcc ggaagtcgcg ccgctcctca gcgatccgcg
     1381 cgttcacatt cacatcgacg atggccgacg ctggctgcgg cgccatccgg aggcaacgtt
     1441 cgacctggtc gtacagaaca cgacctatca ctggcgcgcc aacgcgacca acctgctgtc
     1501 gcgcgagtac ttcgccgagg tgaaatcgca catgcggccc ggcgcgatcg cgacgttcaa
     1561 cacgaccggt tcgctggacg tgctgcgcac tgcgcaggaa gtctttccgg cagcgttcaa
     1621 gttccgctgg ttcgtgtacg tgagcgatca cgacttccgg acaccgcccg atgtcgcgtt
     1681 cgcgcgctt
//
LOCUS       DATANS010000117         1552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_257978, whole genome shotgun sequence.
ACCESSION   DATANS010000117 DATANS010000000
VERSION     DATANS010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257978"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VHT22_01720"
     CDS             complement(<1..358)
                     /locus_tag="VHT22_01720"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX3136298.1"
                     /translation="MTPTLERLRALPMRNLQHRILALFLLLIFVVQVVSLVLINMVGG
                     NSARSTVGAEVAAGARVFDRLLEQDAQRLVQGARLMAADYAFREVIATGDRETIGSVL
                     VNYGKRIDAPLVMIVGL"
     gene            730..>1552
                     /locus_tag="VHT22_01725"
     CDS             730..>1552
                     /locus_tag="VHT22_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136299.1"
                     /translation="MLLPMLLCAARPAYAEWFYDAHAGGLYDDNLTRAQQAADVRGDG
                     AATLAASAGWFHAPSGTDGITLSLDVNSEAYARFHGLNLISVGGSAGYRHKFGLGRDV
                     PWASITVSAAHDDFRGDIRTGDRLEASLEVGKRLSENFDASVGIATDRRFASNDQPVV
                     PGISGKPFDLRGHSVFARAAYGLTERLQVGARVSVRRGDVVSTTRQNLDIFLASDAIA
                     ADPTFGSDFYAYRLRGTTTEAHATLSWALSDRSSLNLSYGVARTAAYDDLNYLSRL"
BASE COUNT          269 a          547 c          481 g          255 t
ORIGIN      
        1 cgagcccgac gatcatcacc agcggcgcat cgatgcgctt gccgtaattg accagcaccg
       61 agccgatcgt ctcgcgatcg ccggtggcga tcacctcgcg gaacgcgtag tcggcggcca
      121 tcaggcgcgc gccctggacc agtcgctgcg catcctgctc gagcaggcga tcgaacacgc
      181 gcgcgccggc ggcgacttcg gcgccgaccg tgctgcgcgc ggaattacct cctaccatgt
      241 tgatcagcac gaggctgacg acctggacga caaagatcag cagcaggaac agcgcaagta
      301 tgcggtgctg caggttgcgc atcggcaacg cgcgcagccg ctcgagcgtc ggcgtcatcg
      361 cgcgctccca ccagtgcccg ccgggcgggc ggagccgatc gatgggaccc tcgcaggact
      421 gccgcgcgtc ggcacatcgg cgaccaagcc ggtcgctgcg cctgcgcaag aaatggacag
      481 cgccgggtct ttcgacccgg cccgtcttgg aaccgggcta tttataatca tattattcaa
      541 taggttacaa atatcgcccg cttcgttcag ccccccggct ttctgctcgc atcaaaaatg
      601 cttgtgcacc acgcgttgcg agcccacgac taagcaagtt ccctgccgcc caaagcgatg
      661 aatgtccagc cgatactgcg ccccgcatgt cgcccgcgcg acgcggccgg cgctcgggcg
      721 gcggcctggg tgctgctgcc gatgctgcta tgcgccgcac gtcccgcgta cgccgaatgg
      781 ttctacgatg cgcacgccgg cggcctgtac gacgacaatc tgacccgggc acagcaggca
      841 gcggatgtgc gcggtgacgg cgccgcaacg cttgccgcca gcgcgggctg gttccacgcc
      901 ccgtccggca ccgacggcat caccctgtcg ctcgacgtca acagcgaagc gtacgcgcgc
      961 tttcatggcc tcaacctgat ctcggtcggc ggcagtgccg gctatcgtca caaattcggt
     1021 ctcggccgcg acgtgccctg ggcatcgatc accgtcagcg ccgcgcatga cgactttcgc
     1081 ggcgatatcc gcaccggcga tcgcctcgaa gcgagcctcg aagtcggcaa gcgcctgagc
     1141 gaaaatttcg acgcctcggt cggcattgcg accgaccgga gatttgccag caacgaccag
     1201 cccgtcgtgc ccgggatctc aggcaagcca ttcgatttac gcgggcacag cgtattcgcg
     1261 cgcgccgcgt acggcctcac cgagcggctg caggtcggcg cgcgcgtgtc ggtgcgccgc
     1321 ggcgacgtcg tgtcgacgac gcgacagaat ctcgacatct ttctggcgtc cgacgcaatt
     1381 gccgccgatc cgacattcgg cagcgacttc tacgcttacc ggctgcgcgg cacgaccacc
     1441 gaagcccacg cgactctgag ctgggcgtta tcggatcgct cctcgctgaa tctcagctac
     1501 ggggtggctc gaacggccgc ctacgacgac ctcaattact tgagccggct cg
//
LOCUS       DATANS010000118         1797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_719671, whole genome shotgun sequence.
ACCESSION   DATANS010000118 DATANS010000000
VERSION     DATANS010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719671"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VHT22_01730"
     CDS             <1..332
                     /locus_tag="VHT22_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3136300.1"
                     /translation="AAAALSHGERRALEIAMALATHPSVLLLDEPLAGMGPEESAHIV
                     ALLKRLAADHAILLVEHDMDAVFALADRLTVMVNGRILASGAPEAIRANAEVQRAYLG
                     TEAVNAL"
     gene            332..1042
                     /locus_tag="VHT22_01735"
     CDS             332..1042
                     /locus_tag="VHT22_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3136301.1"
                     /translation="MTAPLLEARKLHSYYGSSHVLQGVDFSIGAGEVVALMGRNGMGK
                     TTLLRSMLGLLKSRTGEVDIDGVSATDMPPHKIARLGLAFVPENRGIFPTLTVRENLL
                     VAARPAAGGSGWTFERVMTTFPALAARLGHRGNQLSGGEQQMLTIGRALLTNPRLLIL
                     DEATEGLAPLIATTIWRTIRTIRDGGIATVIVDKHFAMLAELADRCVILVKGRKVFDG
                     SPAELAAQPGLREQHLGI"
     gene            1057..>1797
                     /locus_tag="VHT22_01740"
     CDS             1057..>1797
                     /locus_tag="VHT22_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3136302.1"
                     /translation="MPRARFFDVAGHRLECVRIRGAKSAPTLVFLHEGLGSVALWKDF
                     PARVAEATGCPVLVYSRAGYGRSSPAVLPRVPNYMHVEALTVLPALLDRLGIADPVLV
                     GHSDGASIALLHAGSGSRPVRALVALAPHVFVEDMSIASIDEVRRQYETTDLREKLAR
                     RHADPDAAFRGWNDVWLAPAFRSWNIEACLPGVRCPLLLIQGRDDEYGSAAQLDAIER
                     QVGGKVARIELADCRHSPHRDQPEATLAA"
BASE COUNT          280 a          605 c          625 g          287 t
ORIGIN      
        1 tcgccgccgc cgcactgtcg cacggcgaac gccgcgcgct ggagatcgcg atggcgctcg
       61 ccactcatcc tagcgtccta ctgctcgacg agccgctcgc ggggatgggg cccgaagaat
      121 cagcgcacat cgtcgcgctg ctcaagcgcc tggcggcaga ccatgcaatc ctgctggtcg
      181 agcacgacat ggacgcggtg ttcgcgctgg ccgaccggct gacggtgatg gtcaacgggc
      241 gcatattggc gagcggcgcg ccggaggcga tccgcgccaa tgccgaggta cagcgcgcgt
      301 acctgggtac cgaagcggtg aacgcgctgt gatgaccgcg ccattgctcg aagcgcgcaa
      361 gctgcacagc tactacggca gcagccacgt gctgcagggc gtcgactttt cgatcggcgc
      421 cggcgaggtg gtcgcgctga tgggccgaaa cggcatgggc aagacaacct tgctccggtc
      481 gatgctcggc ttgctcaaat cgcgtaccgg cgaagtcgac atcgatggcg tcagcgccac
      541 cgatatgccg ccgcataaga ttgcccgtct cgggcttgcg ttcgtgcccg agaaccgcgg
      601 catcttcccg acgctgacag tgcgcgagaa tctgctggtc gccgcccggc ccgccgccgg
      661 cggcagcggg tggacgttcg aacgggtgat gacgactttt cccgcgcttg ccgcgcggct
      721 cgggcaccgc ggcaaccagc tgtcgggcgg cgagcagcaa atgctgacca tcggccgcgc
      781 actgttgacc aatccccgcc tgctgatcct cgacgaggcg accgagggtc tggcgccact
      841 catcgcgacg acgatctggc ggacgatacg cacgatccgc gacggcggca tcgcgacggt
      901 gatcgtcgac aagcacttcg cgatgctggc cgagctcgcc gaccgctgcg tgatcctggt
      961 aaagggacgc aaggtgttcg acggctcgcc cgccgagctc gcggcgcagc cgggcctgcg
     1021 cgagcagcat ctgggcatct agcgcgcgtt cgatcgatgc cgcgggcgcg atttttcgat
     1081 gtcgccggcc atcggctgga atgcgtgcgc atccgcggag cgaagagcgc accgacgctg
     1141 gtctttctgc acgagggtct cggctcagtg gccttgtgga aggattttcc ggcgcgcgtc
     1201 gccgaggcga ccggctgccc tgtgctggtt tactcgcggg cgggatacgg gcgatcgtcg
     1261 ccggctgtgc tgccgcgtgt gcccaactac atgcacgtcg aggcgctcac cgtcctgccg
     1321 gcgctgctcg accggctcgg gatcgcggat ccggtattgg tcggacacag cgacggcgca
     1381 tcgatcgcgc tattgcacgc gggttcgggc agccgcccgg tgcgcgcgct ggtcgcgctc
     1441 gcgccgcacg tgttcgtcga ggatatgtcg atcgcgagca tcgacgaagt gcgccggcaa
     1501 tacgagacca ccgacttgcg cgaaaagctc gctcgccgcc acgccgatcc cgacgccgcg
     1561 ttccgcggct ggaacgacgt ctggctggcg ccggcgtttc gcagctggaa catcgaggcg
     1621 tgcctgccgg gcgtgcgctg tccgctgctg ctgatccagg gccgcgacga cgagtacgga
     1681 agcgcagcgc agctcgacgc gatcgagcgc caggtcggcg gcaaagtcgc gcggatcgag
     1741 ctcgccgatt gccgtcactc gccgcatcgc gatcagccag aagccacact cgcggcg
//
LOCUS       DATANS010000119         3156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_761514, whole genome shotgun sequence.
ACCESSION   DATANS010000119 DATANS010000000
VERSION     DATANS010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761514"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VHT22_01745"
     CDS             complement(<1..259)
                     /locus_tag="VHT22_01745"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3136303.1"
                     /translation="MHVYDGRYKPAPTSLFPPPDAPLDVYRAVQRQLGLSRAVIVQPN
                     AYAFDNACTEEAVAALGANGRGVATVSPEIADAEIERLTRAG"
     gene            complement(370..2124)
                     /locus_tag="VHT22_01750"
     CDS             complement(370..2124)
                     /locus_tag="VHT22_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3136304.1"
                     /translation="MRWTLVLLALASGCAAGAADPNKVLRVASPDVDTLDPQQATTIP
                     AQRVIQAIFEGLYNWDYLNTPAGLVPVTATAPPEISDGGKTWRINLKPGIHFTDDPAF
                     HRKARELIADDYIYAMKRRLDPNLSRGGDTVMTDLIIGARAAVDAAKRPGSKFDYDRP
                     IEGLRALGRYALQIKLNAINYPSVQIFLTVQAVAREVVEAAGGDIRTHAVGTGPYRLR
                     EWKRGSKIVLEANPDYRPLKFPASSNPADAELVRSMRGRTLPQIGVIDISIIEEDTTL
                     LLEFERGALDYVELRAEIANRLLANDKLKPEYAARGIVRHARLEPFLFFVDFNMIDPV
                     VGGMSNDKIALRRAIAMGLNTQNLIDVVYAGQGQPANQMIPPGVSGHDPGAPAKSLYD
                     PAAAQALLDRFGYKNRDADGYRQMPDGKPLTVVLSLRAAAISRETQTLWKRDMDAIGI
                     RIDFRVAPFQDFVKDLIAGKYQMSAAGFGGVPTGDGILELLDSRQPPFANQSRFKSAE
                     YDQWVDRFLSTPTPSEQVDAARHMNEMARAYMPLLPTIFRRENDFVHPWLQGFRPQVF
                     STYWQFMDIDLDRRKRAG"
     gene            complement(2271..2756)
                     /locus_tag="VHT22_01755"
     CDS             complement(2271..2756)
                     /locus_tag="VHT22_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HEX3136305.1"
                     /translation="MNAIKYVLPLTSALLLGACVTVPTAPTVMALPGSNKTFDEFQAD
                     AYSCQQFAQQMVGGVPVQANNAAAANAVGATALGAAAGAIIGSASGNAGAGAAIGAGT
                     GLLFGSAAGSNYYYGGYYQVQRQYDGAYMQCMYAKGNQIPVRGARPYQAPAHTHYGPP
                     A"
     gene            complement(2770..>3156)
                     /locus_tag="VHT22_01760"
     CDS             complement(2770..>3156)
                     /locus_tag="VHT22_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136306.1"
                     /translation="HGGGGGWHGGHSHVTVGFGFGYPYYWGWGYPYYGYYGYYPYAYG
                     YPYPAAYPYGYYDDGSSSTYIQRDSGEGIAPSYPAPRGEFSAPRDQYSYYCTNPAGYF
                     PQVNNCPSGWLTVVPNAAPHGAPAPR"
BASE COUNT          539 a         1007 c         1032 g          578 t
ORIGIN      
        1 atccggcgcg cgtcagccgc tcgatttcgg catcggcgat ctcgggcgac accgtcgcga
       61 cgccgcgtcc attcgcgccg agcgcggcga ccgcctcctc ggtgcacgca ttgtcgaacg
      121 cgtacgcgtt cggctgcacg atgacggctc ggctgagccc cagctggcgc tgcacggcgc
      181 gatagacgtc gagcggcgcg tccggcggcg ggaacagcga ggtcggcgcc ggcttgtatc
      241 gaccatcgta gacgtgcatg tggcaatcgc aggcgccagc aggcgccatt agccgtggtt
      301 tgtcgtccat tgaattccct tcggcgcgca aggcgcaaca aggtgggttc ttcgggcgcc
      361 tggtacgcgt taaccggccc gcttccggcg atccaggtcg atgtccatga attgccagta
      421 ggtgctgaat acctgcggcc ggaagccctg cagccacgga tgaacgaaat cgttctcgcg
      481 gcgaaatatc gtcggaagca gcggcatgta ggcgcgcgcc atctcgttca tgtggcgcgc
      541 ggcgtcgacc tgctcggacg gcgtcggagt gctgaggaat cgatcgaccc attgatcgta
      601 ttccgccgac ttgaaccgcg actggttggc gaatggcggc tgcctgctgt cgagcagctc
      661 gaggatgcca tcgccggtcg ggacgccgcc gaagccggca gccgacatct ggtatttccc
      721 ggcgatcagg tccttgacga aatcctggaa cggagcgacg cggaaatcga tccggatgcc
      781 gatcgcgtcc atatcccttt tccacaaggt ctgcgtttca cgcgaaatcg cagcggcgcg
      841 aagcgacagg acgaccgtca gcggcttgcc gtccggcatt tggcgatatc catccgcgtc
      901 gcggttcttg tagccgaaac ggtcgagcag cgcttgcgcg gccgcggggt cgtacaacga
      961 cttcgccggc gcccccggat cgtggccgct gacccccgga ggaatcatct ggttggccgg
     1021 ctgcccctgg ccggcgtaca ccacgtcgat caggttctgc gtattcagcc ccattgcgat
     1081 cgcccgccgc aacgcaatct tgtcgttgct catgccgccg acgaccggat cgatcatgtt
     1141 gaaatcgaca aagaagagaa aaggctcgag gcgggcatga cggacgatgc cgcgcgccgc
     1201 gtattcgggc ttcaacttgt cgttggcgag aagacggttg gctatctcgg cgcggagctc
     1261 gacatagtcg agcgcaccgc gctcgaattc cagcagcagc gtcgtgtcct cctcgatgat
     1321 gctgatgtcg atgacgccga tctgaggcag cgtcctgccg cgcatgctgc gcaccagctc
     1381 ggcatccgcc gggttgctgc tcgcgggaaa tttcagcgga cgataatccg gattggcttc
     1441 gagcacgatt ttcgatccgc gtttccattc gcggagccga tatgggcctg ttcccacggc
     1501 atgcgtgcgg atgtcgccgc ccgcggcttc gaccacctcg cgcgcgaccg cctgcaccgt
     1561 caggaaaatc tggaccgacg gatagttgat cgcgttgagc ttgatctgga gcgcgtaacg
     1621 gcccagcgcg cgcagtccct cgatcggacg atcgtagtcg aatttcgagc ccggccgctt
     1681 ggcggcatcg accgccgccc gtgcaccgat gatcaagtcg gtcatcacgg tgtcgccgcc
     1741 gcggctcaga ttgggatcga gccgacgctt catcgcgtag atgtagtcgt cggcgatgag
     1801 ctcgcgcgcc ttgcggtgga acgccgggtc gtcggtgaaa tgaattcccg gcttgagatt
     1861 gatccgccag gtcttgcccc cgtcggagat ttccggaggc gcagttgccg ttaccggcac
     1921 cagtccggcc ggagtattca gatagtccca gttgtacagg ccttcgaaga tcgcctgaat
     1981 gacccgctgc gccggaatcg tggtcgcctg ttgcgggtcc agcgtatcga cgtcgggtga
     2041 tgcgacccgc agcaccttgt tcggatcggc ggcgcctgcg gcgcaacccg aagcgagcgc
     2101 gagcagcaca agcgtccacc gcattcgctt cgtcgcgcga gctgtcaaga atcctccggc
     2161 ggctgcgacg tcctcatcgc aatcgcagga ttatgcatta gcgtgcgtgt cggcgaaaaa
     2221 gaaagggccc gaagcatcgg gcccttgctg tggcgactga aacgctggcg ctacgccggc
     2281 gggccgtagt gggtatgcgc cggtgcctga taaggccgtg cgccacggac ggggatctgg
     2341 ttgcccttgg cgtacatgca ctgcatgtac gcaccgtcat actgacgctg aacctggtag
     2401 taaccgccgt agtagtagtt gcttccggcc gcactgccga acagcaggcc agtcccggcg
     2461 ccgattgccg cgccggctcc cgcgttgccg gacgccgatc cgatgatggc gccggccgct
     2521 gcgccgagcg ccgtggcgcc gaccgcgttc gcggccgcgg cgttgttcgc ctggacagga
     2581 actccgccga ccatctgctg cgcgaactgc tggcagctat aggcatcggc ctggaactcg
     2641 tcgaacgtct tgttcgagcc aggcagcgcc atgacggtcg gggcggtagg caccgtcacg
     2701 caggcgccca gcagcaacgc cgacgtcaac ggcagaacgt acttgatcgc attcatcgtc
     2761 gactcatcct cagcgtggcg ccggcgcacc gtgcggcgcg gcgttcggaa ccaccgtcag
     2821 ccatccgctc gggcagttgt tgacctgcgg gaaatagcct gccggattcg tgcagtagta
     2881 cgagtactgg tcgcgcggtg ccgagaactc tccgcgcggc gccggatatg aaggcgctat
     2941 gccctctccg ctgtcgcgct ggatataggt cgacgaagac ccatcatcgt aatagccgta
     3001 ggggtacgcg gcggggtatg ggtacccgta ggcatacggg tagtacccgt agtacccata
     3061 gtacggatag ccccagcccc agtagtacgg gtagccaaag ccgaatccga cggtgacgtg
     3121 ggaatggccg ccgtgccagc cgccgcctcc gccgtg
//
LOCUS       DATANS010000120         2183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_299685, whole genome shotgun sequence.
ACCESSION   DATANS010000120 DATANS010000000
VERSION     DATANS010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299685"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VHT22_01765"
     CDS             <1..606
                     /locus_tag="VHT22_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEX3136307.1"
                     /translation="VTDNPLVFADSGEVLSGGNFHGEPVALAADVLALAIAEIGALSE
                     RRIALLIDESLSGLPPFLVRDPGINSGFMMAHVTAAALASENKSLAHPASVDSLPTSA
                     NQEDHVSMSTFAARRLGDMTRNSRTIVAIELLAAAQGIDFRAPLKTSQKLQAVHDAVR
                     KRVPFYAEDRYFAPDIGAIGDLVGSGSLTEEVADLLPSWND"
     gene            616..1986
                     /locus_tag="VHT22_01770"
     CDS             616..1986
                     /locus_tag="VHT22_01770"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016395384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEX3136308.1"
                     /translation="MPRRYLASSAMLPSGWADNVLIVVGDDGVIASVATNMSDVAAER
                     LNGPVVPGMPNLHSHAFQRAMAGLTQRAGPEGDSFWAWRELMYRFLARLTPDDVEAIA
                     TQLYIEMLEAGYTSVAEFHYLHHDPEGKAYANPVEMAERIRAAADSAGIGLTLLPVFY
                     AHSNFGGLAPAAGQRRFITDTEQFSRMLDAISKSLANHPMQRLGVAPHSLRAVAPDEL
                     QRLLELREDLNAAMPVHIHAAEQQKEVDDCIAALGARPVQWLLDNSAVDERWCLIHAT
                     HMDRGETGGLAASRAVAGLCPTTEGDLGDGLFSAKSFLANDGRFGIGGDSHVNVDPFL
                     ELRLFEYGQRMKYEQRNVFRLARAESFGGRLYRAACGGGGQALGQNIGAIAAGHRADW
                     IVLDQADSVGQHGDALLDSAIFGPRRAPIGDVMAGGRWHVRDGVHPRHAESAAQYAIT
                     LKRLLT"
     gene            2010..>2183
                     /locus_tag="VHT22_01775"
     CDS             2010..>2183
                     /locus_tag="VHT22_01775"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701032.1"
                     /note="catalyzing the hydrolysis of
                     4-imidazolone-5-propionate to N-formimidoyl-L-glutamate,
                     the third step in the histidine degradation pathway;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX3136309.1"
                     /translation="MWTNVHLATIDQAIGSADGYGSIRDGALAVRDGRIAWVGPRRDL
                     PSGWRAETEHDGGG"
BASE COUNT          369 a          710 c          737 g          367 t
ORIGIN      
        1 gtgaccgaca atcctctggt gttcgccgat tcaggcgaag tgctttccgg cgggaatttc
       61 catggcgagc cggtggcgct cgccgccgat gtcctcgcgc tggccatcgc cgagatagga
      121 gcgctgtcgg agcggagaat cgccttgctg atcgacgagt cgctgtcggg cttgccgccg
      181 ttcctggtgc gcgatccggg catcaactcg ggtttcatga tggcgcatgt caccgccgcg
      241 gcgctggcat cggaaaacaa gtcgctcgcg catccggcca gcgtcgacag cttgccgaca
      301 tcggccaacc aggaagatca cgtcagcatg tcgacgttcg ccgcgcggcg tctcggcgac
      361 atgacgcgga acagccggac catcgtggcg atcgagctgc tcgccgcggc gcagggcatc
      421 gacttccgcg cgccgctgaa gacgtcgcaa aagctgcagg ctgtgcacga cgcggtgcga
      481 aagcgagtgc ccttctacgc cgaggaccgc tacttcgcgc ccgacatcgg cgcgatcggc
      541 gacctggtcg gaagcggcag cctgaccgaa gaagttgccg acttgttgcc ttcctggaac
      601 gactgatcga tttcgatgcc gcgacggtac ctcgcttcca gcgccatgct gccttcgggc
      661 tgggccgaca acgtgctgat cgtcgtcggc gacgacggag tgatcgccag cgtagcgacg
      721 aacatgagcg acgtggccgc cgagcgcctg aacggcccgg tcgttcccgg catgccgaac
      781 ctgcactcgc acgcgttcca gcgtgcgatg gccgggctta cgcaaagggc cggtcccgaa
      841 ggagacagct tctgggcatg gcgggagctg atgtatcgct ttctcgcgcg actgacgccg
      901 gacgacgtcg aagcgattgc gacccagctt tacatcgaaa tgctggaagc cggctacacg
      961 tcggtggccg aatttcacta cctgcatcac gatcccgagg gcaaggctta cgcgaacccg
     1021 gtcgagatgg cggagcggat tcgtgccgca gccgacagcg ccggcatcgg gctgacgctg
     1081 cttccggttt tctatgccca cagcaatttc ggcggtctcg cgccggctgc cggccagcgc
     1141 cgattcatca ccgacaccga gcagttctcg cggatgctcg acgcgatcag caagtcgctc
     1201 gcgaaccatc caatgcaacg cctgggcgtc gcgccgcatt cgcttcgcgc cgtcgcgccc
     1261 gatgagctgc agcgcctgct cgagctccgc gaagatctca atgcggcgat gccggtgcac
     1321 atccatgccg ccgagcagca gaaagaggtc gacgattgca tcgcggcact gggcgcgcgg
     1381 ccggtgcaat ggctgctcga caacagcgca gtggacgagc gctggtgcct gattcacgcg
     1441 acccacatgg accggggcga gaccggagga ttggcggctt cgcgggccgt ggccggcttg
     1501 tgcccgacga cggaaggcga tcttggcgat ggtctgttca gcgccaagtc ctttcttgcg
     1561 aacgatggcc gtttcggcat cggcggcgac agccatgtca acgtcgatcc gtttctcgag
     1621 ctgcgtcttt tcgaatacgg gcagcgcatg aagtacgagc agcgcaacgt attcaggctc
     1681 gcccgcgccg aatcgttcgg cggccgtctc tatcgcgctg cctgcggcgg cggtgggcag
     1741 gcattgggac aaaacatcgg cgccatcgcc gccggccatc gggccgactg gatcgtcctg
     1801 gatcaggccg attccgtcgg acagcacggc gacgcgctgc tcgacagcgc gatcttcggg
     1861 ccacggagag cgccgatcgg cgacgtcatg gcgggcggac gttggcacgt ccgtgacggc
     1921 gtgcatccgc gccacgcgga gagcgcggca caatacgcga tcacattgaa gcgattgttg
     1981 acgtgagcgc cgacctcggc tgggatgcga tgtggaccaa cgtccacctc gcgacgatcg
     2041 atcaggcgat cggcagcgcc gatggctacg ggtcgatccg cgacggtgcg ctcgccgtcc
     2101 gcgacggccg catcgcctgg gtcggcccgc ggcgcgatct tccgtccgga tggcgcgccg
     2161 agaccgagca cgatggcggg ggg
//
LOCUS       DATANS010000121         1507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_48199, whole genome shotgun sequence.
ACCESSION   DATANS010000121 DATANS010000000
VERSION     DATANS010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48199"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VHT22_01780"
     CDS             <1..183
                     /locus_tag="VHT22_01780"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX3136310.1"
                     /translation="AVTTSAGVGIYPDHGENADSLMKSADDALYEAKRAGKNTFRIAQ
                     PDYTVSGAMSASRLRQ"
     gene            complement(265..>1507)
                     /locus_tag="VHT22_01785"
     CDS             complement(265..>1507)
                     /locus_tag="VHT22_01785"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEX3136311.1"
                     /translation="SSGVLRQQPFATTDVVFRQGTLGLASTDPLGAFVTDSAAAGSAM
                     STGVKVVNGAVSITPDGKPVRTAMEVAKASGKRIGLVTTATVYDATPAAFSVHAKSRS
                     EFQSIVDQYFALEPDVLLGGGAEYFLPATAPGGKRKDGKDVIAAFRAKGWQVARNSAE
                     LKAASSAKVLGLFADDDMDFEIDRDAAKEPTTAEMAAAALKALSQLNANGFVLLIENE
                     NTDTAGHANDAAALMRALWAFDDAVKVALDYQRSHPDTLILITGDHETGGFSPTYALK
                     DLSSLASSNRFTVGDTQLKMLERITMSFNTVKEKLGDKPAGPALDELLAKHFPGFRLD
                     PDLRELVLAGRPLERNTSYLPQNTLARMVARQAGFYWGTSGHTPEPVVIGAMGPGEQL
                     FRGYQDNTDFGKSLHRLISGR"
BASE COUNT          266 a          499 c          462 g          280 t
ORIGIN      
        1 gccgtaacca cgagtgcggg tgtcggcatt taccccgacc atggcgagaa tgcggactcg
       61 ctgatgaaga gcgcggatga tgctctgtac gaggccaagc gcgccggcaa gaacaccttt
      121 cgaatcgcgc agccagacta cacagtcagc ggcgcgatga gcgcatcacg cctgcggcaa
      181 taacgaacgc aatcttccgc gcaaccgaac gcaatcgcgc cgctcttcaa acgcacacgc
      241 actcagtcaa agccgaagat ctgtctaacg cccgctgatc agccggtgca ggcttttgcc
      301 gaaatccgtg ttgtcctgat agccgcgaaa cagctgctcg cccggcccca tcgcgccgat
      361 tacgaccggc tcgggcgtgt gcccgctggt gccccagtaa aagccggcct gccgcgcgac
      421 catgcgcgcg agcgtgtttt gcgggagata ggacgtattg cgctcgagcg gccgtcccgc
      481 gagcaccagt tcgcgcaggt ccgggtccag cctgaagccc ggaaaatgct ttgcgagcag
      541 ctcgtcgagc gccggccccg ccggcttgtc accgagcttt tccttcaccg tgttgaacga
      601 catcgtaatg cgctcgagca ttttcagttg cgtatcgccc accgtgaacc ggttgctgct
      661 tgcaagcgag ctcaggtctt tgagcgcata cgtcggcgaa aagccgcccg tctcgtggtc
      721 gcccgtgatc aggatcaggg tatcgggatg gctgcgctga tagtcgagcg cgaccttaac
      781 cgcgtcgtcg aacgcccaca gcgcacgcat gagcgccgcc gcatcgttcg catggccggc
      841 ggtatcggtg ttctcgtttt cgatgagcag cacgaacccg ttggcgttca actgcgacag
      901 cgccttcagg gcagcagccg ccatctcggc ggtcgtcggc tcctttgccg catcgcggtc
      961 gatttcgaaa tccatgtcgt cgtccgcgaa caggccgagc accttggcgc tgctcgccgc
     1021 cttgagctcc gcgctattgc gcgcgacctg ccagcccttg gcgcgaaacg ccgctattac
     1081 atccttgccg tccttgcgct taccgccggg tgcggtcgcc ggcaggaaat attcggcgcc
     1141 accgccgagc agcacatcgg gctcgagcgc gaaatattga tcgacgatgc tttgaaattc
     1201 cgagcgcgac ttcgcgtgca cgctgaacgc cgccggtgtc gcatcgtaca cggtggcggt
     1261 cgtcacgagg cctatgcgct tgccgctcgc tttggcgact tccatcgcag tgcgcaccgg
     1321 cttgccgtcg ggcgtgatcg aaactgcacc gttgacgact ttgacgccgg tcgacatggc
     1381 cgaaccggcc gccgctgaat cggtgacgaa cgcgccgagc ggatcggtgc tcgcgagccc
     1441 gagcgtgccc tgcctgaata cgacgtcagt cgtggcgaat ggctgctggc gcagcacgcc
     1501 gcttgaa
//
LOCUS       DATANS010000122         3514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_468496, whole genome shotgun sequence.
ACCESSION   DATANS010000122 DATANS010000000
VERSION     DATANS010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468496"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VHT22_01790"
     CDS             <1..489
                     /locus_tag="VHT22_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX3136312.1"
                     /translation="GEPVTQLEHALQTAHSAELAGADAALVTAALLHDLGHLLNDQGE
                     TPTARGIDDVHQYAALPFLRPLFDDEVLEAIRLHVDAKRYLCATEPGYGDALSADSKR
                     SLVLQGGPYDQEAAAAFAAQPGALRAIALRRWDDAAKIAGVVTPPLEHFAGIMEAALR
                     RG"
     gene            489..2210
                     /locus_tag="VHT22_01795"
     CDS             489..2210
                     /locus_tag="VHT22_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEX3136313.1"
                     /translation="MQVLLNLLAGVALLVWGTHIVRASILRLYGGDLRRVLRQSVSSR
                     FAAFAAGLSVTALIQSSTATALIVAAFVGQGLIATAPALAVMLGADVGTSLMVLVFSF
                     DLSWLSPLLIFAGVLLYLTRENAKAGRFGHILIGLGLIIMALQLIVLAAKPLTQAAGV
                     QVIFGSLTGDVLLDMLVGATFSILAYSSLAVVLLTAALAASKVIGIHVAMALVLGANL
                     GSGVLAMLNTLRSAPEARRVTLGNFLFKLIGCLAIAPLLRYIEPLLASLDPVESRMVV
                     HFHLLFNVTIAIVFLFFTEQIALLAERLMPAKPDADDPAKPRYLDPSALTTPALAIGC
                     AAREALRIGDVIEQMLTGMLTVLKTNDRVLAERLRKMDDIVDELYTAIKLYLTQISRE
                     ALEDKEGRRWTDIVSFTINMEQVGDIIERVITDVEEKKIEKGRSFSAAGMEEICDLHG
                     RLVANLRLGMSVFLHGDLSSAQQLLAEKVMFRDLERAYAGSHIERLTSNTAQSIETSS
                     LHLDLISDLKRINSHICSIAYPILEQAGVLAKTRLKEPLPASPPDVSASDPGDKPGWQ
                     KRKPHAV"
     gene            2252..3109
                     /locus_tag="VHT22_01800"
     CDS             2252..3109
                     /locus_tag="VHT22_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX3136314.1"
                     /translation="MDLTLGVTLAVLAAGLLHAGWNALLKSSTGGDVLLDTAAVVAGS
                     SLCALALLVFVPLPDPAAWPFIAASTAIHFGYYLTLVQAYRTGDLSFAYPLMRGSAPL
                     IVTVLGSIFLRELPTVQMTAGILLISLGIVSIAFVQRGGAHRRHPAAAAYWAFANAAI
                     IAAYTLVDGAGARASGNALAYVCWLISVEGIPFLVWVVARRRATAFSYLRGCWRAGLG
                     GGACSLLAYGIVLWAMTRAPIAAVAALRETSVLFAALIGSLWLKEGFGWRRVAGAASV
                     VAGIAALKL"
     gene            3165..>3514
                     /locus_tag="VHT22_01805"
     CDS             3165..>3514
                     /locus_tag="VHT22_01805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3136315.1"
                     /translation="MSATIPVQAQVVIVGGGIAGASVAYHLTKLGCSDVVLLEQGKLT
                     CGTTWHAAGLVGQLRATRNATRMSRYGIELYATLEQETGLATGWKQCGSLNIAKTPER
                     LALFKRQMARAKSFG"
BASE COUNT          521 a         1181 c         1173 g          639 t
ORIGIN      
        1 ggcgagccgg tgacgcagct cgagcacgca ttgcagaccg cgcattcggc cgagctggca
       61 ggcgccgacg cggcgctggt cacggcggcc ctgctgcacg acctcggcca tctgctcaac
      121 gaccagggag agacaccgac cgcgcgcggc atcgacgatg tccatcagta tgcggcgctg
      181 ccgtttctgc gcccgctgtt cgacgacgag gtcctcgagg cgatcaggct gcacgttgac
      241 gccaagcgct atctctgcgc caccgagccc ggctacggcg atgcgctgtc ggccgattcc
      301 aagcgcagcc tggtgctgca aggcggcccc tacgaccaag aggcggccgc cgcgttcgcg
      361 gcgcaacccg gggcgctgcg cgcgatcgct ttgcggcgct gggacgatgc ggcaaagatc
      421 gccggggtgg tgacgccgcc gctcgagcat ttcgcaggca tcatggaggc ggcgctgcgc
      481 cgcggctgat gcaggtactg ctcaatctgc tggccggcgt cgccctgctc gtctggggga
      541 cgcatatcgt ccgcgccagc atcctgcgac tctacggcgg cgacctgcgc cgggtgcttc
      601 ggcaaagcgt gtccagccgc ttcgccgcgt tcgccgccgg gctcagcgtc accgcgctga
      661 tccagagcag caccgcgacc gcgctgatcg tcgccgcatt cgtcggccag ggcctgatcg
      721 cgactgcgcc ggcgctggcg gtgatgctgg gcgccgacgt cggcaccagc ctgatggtcc
      781 tggtgttctc gttcgacctt tcctggttgt cgccgctgct gatcttcgcc ggcgtgctgc
      841 tctacctgac gcgcgaaaat gccaaggccg ggcgcttcgg ccacatcctg atcgggctgg
      901 gcctgatcat catggcgctg caactgatcg tgctggcggc aaaacccttg acccaggccg
      961 ccggcgtcca ggtcatcttc ggttcgctga caggcgatgt gctgctggac atgctggtcg
     1021 gagcgacgtt ctcgatcctc gcgtactcga gccttgcggt tgtgctgttg accgcggccc
     1081 tggccgcgtc gaaggtcatc ggcatccatg tggcgatggc gctggtcctc ggcgccaatc
     1141 tgggcagcgg cgtgctggcg atgctcaaca cgctgcgctc ggcgcccgag gcgcggcggg
     1201 tcactctggg caactttctg ttcaagctga tcggttgcct ggcgatcgcg cctttgctgc
     1261 gctatatcga accgctgctg gcttcgctcg atccggtcga gagccggatg gtcgtgcatt
     1321 tccatctgct gttcaacgtg acgatcgcca tcgtcttcct gttcttcacg gagcagatcg
     1381 cgctgctcgc cgagcggctg atgccggcga aacccgatgc cgacgatccg gcgaagccgc
     1441 gctatctcga tccatcggcg ctcacgactc cggcgctcgc gatcggttgc gccgcccgcg
     1501 aggcgctgcg catcggcgat gtcatcgagc agatgctgac cggcatgttg accgtgctca
     1561 agaccaacga tcgggttctc gccgagcggc tgcggaaaat ggacgacatc gtcgacgagt
     1621 tgtacacggc gatcaagctc tacctgacgc agatcagccg cgaagcgctg gaggacaagg
     1681 agggccgccg ctggaccgac atcgtgtcgt ttaccatcaa catggagcag gtcggtgaca
     1741 tcatcgagcg ggtcatcacc gacgtcgagg aaaagaagat cgagaaaggt cgcagcttct
     1801 cggcggcggg aatggaggag atctgcgacc tgcatgggcg gctggtcgcc aacctgcggc
     1861 tgggcatgag cgtgttcctg cacggcgacc tctccagcgc gcagcagctg ctcgccgaaa
     1921 aggtgatgtt ccgcgacctc gagcgtgcct atgccggctc gcacatcgag cgactgacca
     1981 gcaataccgc acagagcatc gagacatcgt cgctgcacct cgacctgatc agcgatctga
     2041 agcgtattaa ctcgcacatc tgctcgatcg cctacccgat cctcgagcag gccggcgtac
     2101 tggccaagac gcgattgaag gagccgttgc cggcgtcgcc gcccgatgtc tcggcgtcgg
     2161 atcccggcga caagccgggc tggcagaagc gcaagccgca cgccgtctag ggcctggcga
     2221 aaatgcgtgc ccgcgtgacc acccggccct gatggatctc acgctgggcg tcactttggc
     2281 ggtgctcgcc gccggcttgc tgcacgccgg ctggaatgcg ctgctcaaat cgtcgaccgg
     2341 cggcgacgtg ctgctggaca ccgcggcggt ggtcgctggt tcgtcgctgt gcgcgctcgc
     2401 gctgctggtc ttcgtccccc tgcccgatcc cgctgcgtgg ccgttcatcg cggcatcgac
     2461 ggcgatccat ttcggctact acctcacgct cgtgcaggcg taccggaccg gcgatctctc
     2521 gtttgcctat ccgctgatgc gcggcagcgc gccgctgatc gtcacggttc tgggcagcat
     2581 attcctgcgc gagctgccga ccgtgcaaat gaccgccggc atcctgctga tctcgctggg
     2641 catcgtcagc atcgcgttcg tgcagcgagg cggtgcacac cgtcggcacc ccgctgccgc
     2701 cgcgtactgg gctttcgcga acgctgcgat cattgctgct tatacgctcg tcgacggcgc
     2761 cggcgcccgg gcatccggca atgcgctggc gtacgtctgc tggctgatct ccgtcgaagg
     2821 catccctttc ctcgtctggg tggtggcgcg ccgtcgtgca acagcttttt catatttgcg
     2881 cggctgctgg cgcgccggac tgggcggagg cgcgtgcagc ttgctcgcgt acggcatcgt
     2941 gctctgggcg atgacgcgcg cgccgatcgc cgcggtcgct gcgcttcgtg aaacctcggt
     3001 ccttttcgcc gcgctcatcg gctcgctctg gctcaaggaa ggcttcggct ggcgtcgcgt
     3061 cgccggtgcg gccagcgtcg tggccggcat cgctgccttg aagctatgat cgcgccaata
     3121 cagctccggt gtccctccat cgcagcggca agcggattgc gccggtgagc gcgactatcc
     3181 cggtgcaggc gcaggtggtg atcgtcggcg gcggcatcgc cggcgcgtcg gtcgcgtatc
     3241 acctgaccaa gctcggctgc agcgacgtcg tgctgctcga gcagggcaag ctgacgtgcg
     3301 gcaccacctg gcacgccgcg gggctggtcg gccagcttcg cgcgacacgc aacgcgacgc
     3361 ggatgagccg ctacggcatc gagctctatg cgacgctcga acaggaaacc gggctcgcga
     3421 ccggatggaa gcagtgcggc tcgctcaaca tcgcgaagac gcccgagcgc ctcgcgctgt
     3481 tcaagcgcca gatggcgcgc gcgaagagct tcgg
//
LOCUS       DATANS010000123         1651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_48334, whole genome shotgun sequence.
ACCESSION   DATANS010000123 DATANS010000000
VERSION     DATANS010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48334"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VHT22_01810"
     CDS             complement(<1..622)
                     /locus_tag="VHT22_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX3136316.1"
                     /translation="MSPSLVLYRRLLTYVRPYWWAFALALIGMVIVAAGDTLMAYMVM
                     PIIQKLQNPDPAMTVQLPLAVVAVFLLRGLGSFMSEYGMAWTGHRVVFDLRREMIDRL
                     LSLPTPYYDAQSSGRLISKFTFDAYQLAAATSSAITTAVRSALTIAGTLGLLFWLNWQ
                     LTLVSIVIMPAVAVVIRYFSRRLRRVARDVQQRTGSMSHVLEEIVGA"
     gene            complement(690..>1651)
                     /locus_tag="VHT22_01815"
     CDS             complement(690..>1651)
                     /locus_tag="VHT22_01815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3136317.1"
                     /translation="AVCRAVTQKAPELVQSHERLLCCDVYRAGDGVHRSWLDERLRGA
                     STLRRWWTELSPYHRYVLAIERRLFASPLLKAVICNSRMVRQDIRDRFGLPDERLPVV
                     YNAVDSEAFSPKLRAHRDSMRQKLGVPPDATLFLLVGSGYVRKGVAAALTALSRLPDN
                     THLLVVGRDKARGRYKRMARRLGIASRVALVGAQDDPKPFYGAADVFVLPTLYDPCPN
                     AALEAMACGLPVVTSTKCGAAELVLEHDAGFVCDAGDLDALTAHMSTLTDAEARTGRG
                     ERAREAVLTLTPEAMTLQLVLLYRDLLAAAHAARTEQPKTAAL"
BASE COUNT          297 a          558 c          559 g          237 t
ORIGIN      
        1 gcgcgccgac gatttcctcg agcacatggc tcatcgagcc ggtgcgctgc tggacgtcgc
       61 gcgccacgcg gcgcagccgc cggctgaaat agcggatcac gaccgcgact gccggcatga
      121 tgacgatcga aaccagcgtc agctgccagt tgagccagaa cagaaggcca agcgtcccgg
      181 caatcgtcaa tgcgctgcgc accgcggtcg tgatcgccga cgaggtcgcg gcggcgagct
      241 ggtaggcgtc gaacgtgaac ttcgaaatca gccggcccga cgactgagcg tcgtaatacg
      301 gcgtcggcag cgacagcagg cgatcgatca tctcgcgccg caggtcgaac acgacgcggt
      361 ggccggtcca cgccatgccg tactcggaca tgaacgagcc caatccgcgc agcaggaaca
      421 ccgcaaccac cgccagcgga agctgcaccg tcatcgcagg atcggggttc tgcagcttct
      481 ggatgatcgg catcaccatg taggccatca gcgtatcgcc ggcggcgacg ataaccatgc
      541 cgataagcgc caacgcgaac gcccaccagt aggggcggac gtaggtcagc agacgccggt
      601 agaggaccag gctgggcgac acggcgggtc cgatgcggcc ggcgcctggt gttcctcggg
      661 cggccggcgg tggtatgaac gggccgcgat tatagcgcag cggtcttcgg ctgctcggtc
      721 ctggccgcgt gcgccgcggc caggagatcg cgatagagca gcaccagttg cagcgtcatc
      781 gcctcgggcg tcagcgtcag caccgcttcg cgcgcgcgtt cgccccgtcc ggtccgcgcc
      841 tctgcatcgg tcagcgtgct catgtgcgcg gtcaaggcgt cgaggtcgcc cgcatcgcag
      901 acgaatcccg catcgtgctc gaggacgagc tcggcggcgc cgcatttggt gctggtcaca
      961 acaggaagtc cgcaggccat cgcctcgagc gcggcgttcg gacatggatc atagagcgtc
     1021 ggcagtacga agacgtcggc cgcgccgtag aacggcttcg gatcgtcctg cgcgccgacc
     1081 agtgcgaccc gcgatgcgat gccgagccgc cgtgccatcc gcttgtatcg cccccgcgcc
     1141 ttgtcgcggc cgacgaccaa cagatgcgta ttgtccggga gccgcgacaa tgcggtgagc
     1201 gcggcggcca cccctttgcg cacatagccg gagccgacca gcaggaacag ggtcgcgtcc
     1261 ggcggcacgc cgagcttctg ccgcatcgag tcgcgatgcg cgcggagctt cggcgaaaac
     1321 gcttcgctgt cgacggcgtt gtagacgacc ggaaggcgct cgtcgggcag cccgaagcga
     1381 tcgcggatat cctgccggac catccgcgaa ttgcagatca ccgccttcag caacggactc
     1441 gcgaacagcc gccgctcgat cgcgagcaca tagcgatgat aaggactgag ctcggtccac
     1501 cagcggcgga gcgtcgaggc gccgcgcaac cgctcgtcga gccaggagcg gtgcacgcca
     1561 tcgccggcgc ggtacacgtc gcagcacagc agccgctcgt gcgactggac gagctccggg
     1621 gctttttggg tcactgcccg gcacactgcg c
//
LOCUS       DATANS010000124         3085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_677531, whole genome shotgun sequence.
ACCESSION   DATANS010000124 DATANS010000000
VERSION     DATANS010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677531"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VHT22_01820"
     CDS             complement(<1..1028)
                     /locus_tag="VHT22_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HEX3136318.1"
                     /translation="MSRKTGFTTSILHADREAAVEHGALHKPLHLSVAYGYRDARELA
                     AVFQGRAQGYVYGRQGNPTTAALEEKINRMEDGVATVCFGTGMAAIGAILLALLREND
                     HVVSSAFLFGNTNSMFNTLATHGDRVSFVDATSADAVEAALTPETRIVFVETIANPRT
                     QIADLARIGELCAARGVVYVVDNTMTSPYLFRPKTVKAALVVNALTKYIGGHGNALGG
                     SITDTGGFDWTRYPNIYDDYKGPKPELWGITQIRKKGLRDWGGTLAAEPAHHLAIGAE
                     TLALRMQRQCANALSLARFLEARSEVRAVYYPGLASHPQHRLAGELFTDYGALLSFEL
                     DPALDCFD"
     gene            complement(1216..2712)
                     /gene="purF"
                     /locus_tag="VHT22_01825"
     CDS             complement(1216..2712)
                     /gene="purF"
                     /locus_tag="VHT22_01825"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX3136319.1"
                     /translation="MCGIIGAVTHTPVNQLLYDGLLLLQHRGQDAAGIVTSEGPVFHM
                     YKGPGYVRDVFRTRNMRELMGNTGIGHCRYPTAGSASSDLESQPFYVNSPFGITLAHN
                     GNLTNSEALKRELFQLDFRHVNTNSDSEVLLNVLALELERAAHHAKLDANAIFAAVAA
                     VHKRCRGAYAIVAMIAGYGLLAFRDPFGIRPLIIGVNESMGGTEYVVGSESVALEALG
                     FSVLRDVAPGEAIYVDQTGKFHARQCAENPQLMPCIFEFVYLARPDSVIDGASVYESR
                     LRMGGELAKKIRGMPEAQDIDVVIPIPDSSRPSALELANSLGLKYREGFVKNRYIGRT
                     FIMPGQAIRRKSVRQKLNPMGIEFKDKVVLLVDDSIVRGTTSREIVQMAREAGARKVY
                     FASASPPVRYPNVYGIDMPTARELVATGRTEAEIANEIGADLLIYQDLEALKRAVRGA
                     NPRLKEFEASCFDGYYVTGDVTADYLSHLARERDETRGESEESLLAAG"
     gene            complement(2740..>3085)
                     /locus_tag="VHT22_01830"
     CDS             complement(2740..>3085)
                     /locus_tag="VHT22_01830"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX3136320.1"
                     /translation="AFVLIIVVVLLVGALLGWLLSRLVRAVGLGFLDRSLGAVLGLAR
                     GLLIVVIGVLIAGLTTFPQQEWWRHAMFAPALVDAALSLRPWLPQGWAQQLAFPKPEK
                     RSTKQVLQAGAT"
BASE COUNT          556 a         1004 c          995 g          530 t
ORIGIN      
        1 aaatcgaaac agtcgagcgc cggatcgagc tcgaacgaca gcagtgcgcc gtaatcggtg
       61 aagagttcgc ccgccagccg gtgctgcgga tgcgaggcca atcccggata gtagacggcg
      121 cgcacctcgg accgcgcctc gaggaagcgc gccagcgaaa gcgcgttcgc acattgcctc
      181 tgcatgcgca aggccagcgt ctccgcaccg atggcgaggt gatgcgcggg ctcggcggcc
      241 agcgtcccgc cccagtcgcg aaggcccttt ttcctgatct gcgtaatgcc ccaaagctcg
      301 ggcttgggcc ccttgtagtc gtcgtagatg ttcggatagc gcgtccagtc gaagccgccg
      361 gtgtcggtga tgctgccgcc caacgcgttg ccatgacctc cgatgtattt ggtcagcgcg
      421 ttgacgacca gcgcggcctt gaccgttttc ggacgaaaca ggtacggcga agtcatcgta
      481 ttgtcgacga cgtagacgac gccgcgcgcg gcgcagagct cgccgatccg cgccaggtcg
      541 gcgatctgag tccgcggatt ggcgatcgtc tcgacgaata cgatgcgcgt ttccggcgtc
      601 aacgccgcct cgacggcgtc ggcactggtc gcatcgacga agctcacccg gtcgccgtgc
      661 gtagctaggg tgttgaacat gctgttggtg ttgccgaaca ggaacgcgct ggagacgacg
      721 tgatcgttct ctcgcagcag cgcgagcagg atggcaccga tcgcggccat gccagtgccg
      781 aagcacacgg ttgcgacccc gtcctccatg cggttgatct tttcctcgag cgcggcggtg
      841 gtcgggttgc cctgccggcc gtaaacgtag ccctgcgcac ggccctggaa taccgcggcc
      901 agctcgcgcg cgtcgcgata gccataggct accgacaggt gcagcggctt gtgcaaagcg
      961 ccgtgctcga cggcggcttc gcggtcggcg tgcagaatcg aggtggtgaa tccggtcttg
     1021 cgcgacatcg gatcgctcca gcaaaaaggc ccgtcagctt gcgctaaacg ggccccggat
     1081 cgattcgtcg cctcgcttta gctgaatttg tatcgcgccc gcaagctgtg aagccgccgt
     1141 tgccggcaac tccgattcaa atcggcgctc tggtttgaag tatcgccgca aaccgcggat
     1201 cagtcaatgc ccgcttcagc cggcggcgag cagcgattcc tcgctttcgc cccgcgtctc
     1261 gtcgcgctcg cgcgctagat gcgacaggta atcggcggtg acgtcgccgg tgacgtagta
     1321 gccatcaaag cacgaagcct cgaattcctt cagccgcgga ttggcgccac gcaccgcacg
     1381 cttcagcgcc tcgagatcct ggtagatcag gagatcggcg ccgatctcgt tcgcgatctc
     1441 ggcttcggtg cgtccggtcg ccaccagctc gcgcgcggta ggcatgtcga tgccatagac
     1501 gttcggatag cgcaccggcg ggctggccga agcgaaataa accttgcgcg cgccggcctc
     1561 gcgcgccatc tgcacgatct cccggctggt cgtcccgcgc acgatcgagt cgtcgaccaa
     1621 cagcacgacc ttgtccttga attcgatgcc catcgggttg agcttctggc gcactgactt
     1681 acggcggatc gcctgcccgg gcatgatgaa cgtgcggccg atatagcgat tcttgacgaa
     1741 gccctcgcga tacttgagcc ccagcgagtt ggcgagctcc agcgcagacg gccggctgga
     1801 gtcgggaatc ggaatgacga cgtcgatatc ctgcgcctcg ggcatgccgc ggatcttctt
     1861 cgccagctcg ccgcccatcc gcaatctcga ctcgtagacc gacgcgccgt cgatcaccga
     1921 gtccggccgg gcgaggtaga cgaactcgaa gatgcaaggc atcagctgcg gattctccgc
     1981 gcactgccgc gcgtggaatt tgccggtctg gtcgacgtag atcgcttcgc ccggcgccac
     2041 gtcgcgcagc accgaaaagc ccagcgcctc gagggcgacc gattcgctgc cgacgacgta
     2101 ttcggtgccg cccattgatt cgttgacgcc gatgatcaac ggccgaatgc cgaacggatc
     2161 gcgaaaagcg agcaatccgt agccggcgat catcgcgacg atcgcatacg cgccgcggca
     2221 ccgcttgtgc accgctgcga ccgcggcgaa gatcgcgttg gcgtcgagct tggcatgatg
     2281 cgcggcacgc tcgagctcga gtgccagcac gttgagcagg acctccgaat cggagttggt
     2341 gttgacgtgg cgaaaatcga gctggaacag ctcgcgcttc agcgcctcgc tgttggtcag
     2401 gttgccgttg tgcgcgagcg tgatgccgaa cggcgagttg acgtagaacg gctgcgattc
     2461 gaggtcggag gaggccgatc cggccgtcgg gtagcggcaa tggccgatac cagtattgcc
     2521 catgagctcg cgcatgttgc gcgtgcgaaa cacgtcgcgc acatatcccg ggcccttgta
     2581 catgtgaaag accgggcctt cgctggtcac gatgccggcc gcgtcctggc cgcgatgctg
     2641 cagcagcagc agcccgtcat agagcagctg gttgacgggc gtatgagtga cggcgccgat
     2701 gattccgcac atgtcagtgc cctgccccgc agatagccac taggtcgctc ccgcctggag
     2761 gacctgcttg gttgacctct tctcgggctt cggaaaggcg agctgctggg cccatccctg
     2821 cggcagccag ggcctcagac tcagagccgc atcgaccagc gcgggcgcga acatcgcatg
     2881 gcgccaccac tcctgctgag gaaaggtcgt caggccggcg atcagcacgc cgatgacgac
     2941 gatcagcagt ccgcgagcga gacccagcac cgcgcccagc gacctgtcga ggaagccaag
     3001 gccaaccgcg cgcaccagcc gcgacagcag ccagccgagc agcgcgccga cgagcagcac
     3061 gacgacgatg atcagcacaa acgcg
//
LOCUS       DATANS010000125         1741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_427042, whole genome shotgun sequence.
ACCESSION   DATANS010000125 DATANS010000000
VERSION     DATANS010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427042"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VHT22_01835"
     CDS             <1..249
                     /locus_tag="VHT22_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136321.1"
                     /translation="APRPATLDVKVLGLLPNWRPSAIHILRSLGELLQERYKLKAVIM
                     EQPVRELPLRDGKLLDTMKERLDNLVQRCDVVITASGD"
     gene            313..573
                     /locus_tag="VHT22_01840"
     CDS             313..573
                     /locus_tag="VHT22_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136322.1"
                     /translation="MMVVTDMFAKFARRMSSTQGFPYVVIAETPNPIRQLEPEAMRPR
                     AQAMIQNVIDGLTLPPAELQARMQDVIRQIHPQGIARSTVPI"
     gene            586..1599
                     /locus_tag="VHT22_01845"
     CDS             586..1599
                     /locus_tag="VHT22_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136323.1"
                     /translation="MIQTQLEGASVADLLTRSYEHAYEQGWTDGLPIIPATPEAVAKF
                     VAASGREADELIALLPPRKGRATVEVIAVNAIMAGCRPEYMPVVIAAVEGVSDPKYPL
                     ELMQVTTNPMTPFLLVNGPVRDKLEINYGTGCLGPGWRANATIGRALRLTLINAGGAL
                     PGVYSKTSFGSPLRYSYICGENEEANPWTPFHVDRGFKREDSAVTAFKASNFCNISGG
                     EGVGAEEILRQIATNMPPMYGGGDGVLLLLGVNHAQSLFEAGLTKRAVQQRLWELARL
                     PISHFAEAFSSTERAHGRGDDKSVWRASSPDEIYIAVAGGAGPQNVYIGAGMPQTRVV
                     RGL"
     gene            complement(1605..>1741)
                     /locus_tag="VHT22_01850"
     CDS             complement(1605..>1741)
                     /locus_tag="VHT22_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004205409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxidase"
                     /protein_id="HEX3136324.1"
                     /translation="QLKRMTGGEDGVIDALFRFTQPVTGAYFWCPPMKGGRLDLTAIG
                     "
BASE COUNT          334 a          574 c          549 g          284 t
ORIGIN      
        1 gcgccccgcc ccgccacact cgacgtcaag gtactgggcc tcttgcccaa ctggcggccg
       61 tccgccatcc acatcctgcg ctcgctcggc gaactcctac aggaacgcta caaactgaaa
      121 gcagtgatca tggagcaacc cgtgcgcgag ctgccgctgc gcgacggcaa actgctcgac
      181 acgatgaagg agcggctcga caacctggtc cagcgctgcg acgtcgtcat caccgccagc
      241 ggcgattgag ggtcgtgtac gacgtggtgt agccacgtca cgcccgccct cgaagaacgc
      301 ggcatccccg cggtgatggt cgtcacggac atgttcgcca agttcgcgcg gcgcatgtcg
      361 tcgacacaag ggtttccgta tgtcgtgatc gcggaaacgc cgaacccgat ccgccagctc
      421 gaacccgaag cgatgcgccc gcgcgcgcaa gccatgatcc agaacgtcat cgacggcctc
      481 acgctgccgc ccgccgaact gcaggcgcgc atgcaggacg tgatacggca aatccatccg
      541 cagggcatcg cgcgctcgac ggtgcccatc taggaaagaa cgccagtgat tcaaactcaa
      601 ctcgaaggcg catcggttgc agatctcctc acacgcagtt acgagcacgc atacgagcag
      661 ggttggaccg acggcctgcc gatcattccg gccacacccg aggccgtcgc gaaattcgtc
      721 gctgcgtcgg ggcgcgaggc cgacgagctg atcgcgctct tgccgccgcg caagggccgc
      781 gcgacggtcg aagtaatcgc ggtgaacgcg atcatggccg gctgccggcc cgaatacatg
      841 ccggtggtga ttgcggcggt cgaaggcgtg agcgacccga aatatccgct cgaactgatg
      901 caggtgacga ccaaccctat gacgccgttt ctgctcgtga acggcccggt gcgcgacaag
      961 ctcgaaatca attacggcac cggctgcctg ggccccggct ggcgcgcgaa cgccaccatc
     1021 ggccgcgcgc tgcgcttaac gcttatcaac gccggcggcg cgctgcccgg cgtgtattcg
     1081 aaaacaagct tcggctcgcc cttgcgttat tcatatatct gcggcgagaa cgaggaagcg
     1141 aatccgtgga cgccgtttca cgtcgaccgc ggcttcaagc gcgaggacag cgcggtcacg
     1201 gcgttcaagg cttccaattt ctgcaatata tcgggcggcg aaggtgtcgg cgctgaagag
     1261 attctgcgcc agatcgcgac caacatgccg ccgatgtacg gcggcggcga cggagtactg
     1321 ctgctgctcg gcgtcaacca cgcacagagc ctgtttgaag cggggctcac caagcgcgcc
     1381 gtccaacagc gcctgtggga gctcgcgcgg ctgcccatct cgcatttcgc cgaagcgttt
     1441 tcgtcgacgg agcgcgccca cgggcgcggc gacgacaaga gcgtgtggcg cgcgagcagt
     1501 ccggacgaaa tctatattgc ggttgcggga ggcgcgggcc cgcagaatgt ttacattggc
     1561 gcgggcatgc cgcaaacacg ggtcgtccgc ggcttgtgag cctgctaccc gatcgccgtt
     1621 agatccagcc gtccgccttt catgggcggg caccagaagt acgcgccggt gacgggctga
     1681 gtaaatctga acagtgcatc gatgacgccg tcttcgccgc cggtcatgcg cttcaactgc
     1741 g
//
LOCUS       DATANS010000126         7252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_803386, whole genome shotgun sequence.
ACCESSION   DATANS010000126 DATANS010000000
VERSION     DATANS010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7252
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803386"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1716
                     /gene="ppk1"
                     /locus_tag="VHT22_01855"
     CDS             <1..1716
                     /gene="ppk1"
                     /locus_tag="VHT22_01855"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX3136325.1"
                     /translation="WVADYFRREVKPLLSPIGLDPAHPFPQVVNKSLNFIIELSGSDA
                     FGRETAIAIVKAPHQLPRVIELPTEVAPANSFVLLASIIHAHLGELFAGRRVIGYSQF
                     RVTRDADLWFDEEEVKNLRQALEGELPQRQFGLAVRLEVAADCAPPLAQLLLQQFELE
                     EADLYRCDGPVNLARLTTLVDESERTELKYPPFVPGTPVRLRDQPDILAAIRQHDILL
                     HHPYQSFDPVVEFIRKASDDPDVVAIKQTVYRTGVNSILMDALVEAARRRKEVTVVVE
                     LLARFDEEANINWAERLEEAGAQVVYGVFGLKTHAKLALLLRREIDAAGRSQLRPYAH
                     LGTGNYHPRTARQYTDFGLLTVNPELCADVAEVFLHMTSLARSNRLKRLWLAPFTMHR
                     QLLAAIHRETRHAKEGVPAHIMAKMNGLIEEAVIRALYAASQAGVKIDLVVRGACALR
                     PGVKGWSDNIRIRSIVGRFLEHPRIWYFANGGREDVYLSSADWMGRNLFRRIEVAFPV
                     LDKELKARVIAEGLAVCFEDNCNAWDLGSDGKYVRAKPARRARASAAQELLLDRLAER
                     PSTVR"
     gene            1734..2186
                     /gene="sixA"
                     /locus_tag="VHT22_01860"
     CDS             1734..2186
                     /gene="sixA"
                     /locus_tag="VHT22_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEX3136326.1"
                     /translation="MDLILWRHADAEPGEPDIDRPLTAKGVRQAERMGAWLDSHLPDH
                     CRILVSPAERTQQTARALGRKFKTAPELAPGATAAAVLAAAGWPDAREARLIVGHQPT
                     LGETAALLLSGSDAPWGMRKAAVWWLSNRSREQGADVVLKAVIGPDFL"
     gene            complement(2225..3676)
                     /locus_tag="VHT22_01865"
     CDS             complement(2225..3676)
                     /locus_tag="VHT22_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX3136327.1"
                     /translation="MTAKTLAVVDLGSNSFRLEIGRVEGDQIYRQDTWRETLRFGAGI
                     DRDGRIKPAAIKVALACLARFRERLSGVHPSAVRAVATSAFRVAANAREFLPKAERAL
                     GSRIDVISGHEEARLIYLGVAHELPPSDAQRLVVDIGGGSTEFIIGRGLEPDRLESLT
                     LGCVSTTQKFFPDGMVTAGALDAAETAARVEIEAIAGDFGAGHWKSAYASSGTAAALA
                     EILEQNGFSGGGITPAGLDRLKKRLVRARDPARLKLNALKPERVPVLAGGLAIMSAAV
                     TELDIERIDPVGGALRLGVLYDLLGRTVDRDARQLTVERFLDRYQVDRRHATRVDALA
                     GALFRRAAPKAGPEQIQMLHWAALLHEIGMSVSHIGFHKHGAYILQHADMPGFSAGEQ
                     ARLALLVYGCRGGLTKVRAELGDPVARAQIAALRLAVLFCHARTEVALPHVALKTRGG
                     VTIRVSKRWLAAHPLTAYLLERERSQWQGLHAE"
     gene            3828..5342
                     /gene="ilvA"
                     /locus_tag="VHT22_01870"
     CDS             3828..5342
                     /gene="ilvA"
                     /locus_tag="VHT22_01870"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEX3136328.1"
                     /translation="MPIDYLQRILNAKVYDVAIESPLEPAPMLSRRIGNRVLLKREDK
                     QEVFSFKLRGAYNRMAHLSSAERARGVVAASAGNHAQGVALAAQRLGCKATIVMPVTS
                     PSIKIQAVEERGATVVLHGDSYSDAYAHAAKLQRARRATFIHPYDDPDVIAGQGTIAV
                     EILRQCQGPLDAIFVAVGGGGLIAGIAAYVKRVRPEVRVIGVQPVDSDAMTRSLAAGR
                     RIRLGHVGLFADGVAVKQVGKETFRICRELVDDMITVDTDAICAALKDVFEDTRTVLE
                     PAGALSVAGVKAWCERHRPKRRTFVAIACGANMNFDRLRFVAERAELGEAREAIFAVT
                     IPERPGSFREFCALLGKRSVTEFNYRYADPHVAHIFVGVEVANRHETAALLRAMHARR
                     IEAYDLSDNEMAKLHVRHLVGGHAPAAENEILYRFEFPERPGALMRFLNSMSRGWNIS
                     LFHYRNHGADHGRVLVGMQVPPADHANFRGFLKRLGYYGVDETANPAYRMFLGR"
     gene            5758..6120
                     /locus_tag="VHT22_01875"
     CDS             5758..6120
                     /locus_tag="VHT22_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3136329.1"
                     /translation="MVIDDSNTIRRSAEIFLLQAGCTVILAEDGFDALAKITDHQPDL
                     IFVDIMMPRLDGYQTCALIKKNSRFHQTPVVMLSSKDGLFDRARGRMVGSDQYLTKPF
                     TKESLLKAVGTHVKSAAH"
     gene            6171..6536
                     /locus_tag="VHT22_01880"
     CDS             6171..6536
                     /locus_tag="VHT22_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3136330.1"
                     /translation="MTIKRVLIVDDSPTERHVLNDMLTKAGYEVVASDNGEDAILKAK
                     TVKPDLILMDVVMPGLNGFQATRAISRDPATRSVPIILCTSKTQETDKIWGMRQGARD
                     YIVKPVNRDELIAKITALG"
     gene            6546..7076
                     /locus_tag="VHT22_01885"
     CDS             6546..7076
                     /locus_tag="VHT22_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX3136331.1"
                     /translation="MARAAKLDLRAFQQELAARLAAKTTQQVEQSRLGLACAGKQWLI
                     RLADAGEVIAVPTMATVPLTRSWYLGISNIRGNLYSVIDFAGFLGEAIEPLTLGAAQA
                     RLVLFGPRVGELRAGLVVTRVLGLSNLAELVRSDAAADAPAWYGARWTDKAGGVWQEI
                     DLALLAQDPGFLQVGL"
BASE COUNT         1279 a         2370 c         2395 g         1208 t
ORIGIN      
        1 tgggtcgccg attacttccg gcgcgaggtc aagccgctgc tgagcccgat cgggctcgat
       61 ccggcgcacc cctttcccca agtcgtcaac aagagcctca acttcatcat cgagctttcc
      121 ggcagcgacg ccttcgggcg cgaaacggct attgcgatcg tcaaggcgcc gcaccagctg
      181 ccgcgtgtga tcgagctgcc cacggaggtc gcgccggcca acagttttgt gctgctggcc
      241 tcgatcatcc acgcgcatct gggcgagctg ttcgccggac ggcgcgtcat cggctattcg
      301 caatttcgtg tgacgcgcga cgccgatctg tggttcgacg aggaggaggt aaagaatttg
      361 aggcaggcgc tcgagggcga gctgccgcag cggcaattcg gtcttgccgt gcgcctggag
      421 gtcgctgccg attgcgcgcc accgcttgcc cagctgctgc tgcagcaatt cgagctcgag
      481 gaggctgatc tgtatcgatg cgacggtccg gtcaatctcg ccaggttgac gactctggtc
      541 gacgagtcgg aacgcaccga gctcaagtat ccgccgttcg tccccggcac gccggtccgg
      601 ctgcgcgacc agcccgacat cctggccgca atccggcagc acgacatcct gctgcatcat
      661 ccgtatcagt cgttcgaccc ggtggtcgag ttcatccgca aggccagcga cgaccccgac
      721 gtggtggcaa tcaagcaaac cgtctatcga accggcgtca attcgatact gatggacgcg
      781 ctggtcgagg cggcgcgccg gcggaaggaa gtcaccgtcg tcgtcgagct cctggcgcgg
      841 ttcgacgagg aggcgaacat caactgggcc gagcgtctgg aggaagccgg cgcgcaggtc
      901 gtgtatgggg tattcggcct gaagacccac gccaagctcg cgctgctgct gcgccgggag
      961 atcgatgctg cgggccgctc tcagctgcgg ccgtatgccc atctgggcac cggaaactac
     1021 cacccacgta ccgcgcggca atacaccgac ttcggcttgc tcaccgtgaa tccggaattg
     1081 tgcgccgatg tcgccgaggt cttcctgcac atgaccagcc tggcccgctc gaaccggctg
     1141 aaacgcctct ggctggcgcc attcaccatg catcgtcaat tgcttgcggc aatacatcgc
     1201 gagacgcggc acgccaagga aggtgtcccc gcgcatatca tggccaagat gaacggcctg
     1261 atcgaggaag cggtgatcag ggcgctctat gccgcttccc aggccggcgt gaagatcgac
     1321 ctggtcgtgc gcggcgcgtg cgcgctgcgt cccggggtca aggggtggtc cgacaacata
     1381 cggatccgct cgatcgtcgg acgctttctc gagcacccgc gcatctggta tttcgcgaac
     1441 ggcggccgcg aggacgtcta tctgtcgagc gccgactgga tgggacgaaa cctgtttcgc
     1501 cgcatcgagg tggcatttcc ggtgctcgac aaggagctca aggcgcgcgt catcgccgaa
     1561 gggctggcag tgtgtttcga ggacaattgc aacgcctggg atctgggctc cgacggtaaa
     1621 tatgtgcgtg cgaagcccgc gcggcgcgcg cgcgcctccg ctgcgcagga actcctgctc
     1681 gatcggctgg ccgaacgtcc gagcaccgta cgctgaatgg agccgctgtc atcatggacc
     1741 tgatcctctg gcgccacgcc gacgccgagc ctggcgaacc cgatatcgac cgcccgctga
     1801 cggccaaagg cgtccgacaa gccgagcgca tgggcgcctg gctcgacagc cacctgcccg
     1861 accattgccg cattctggtg agccctgccg aacggaccca gcagaccgca cgcgcgctcg
     1921 ggcgaaagtt caagacggcg cccgagctcg cgcccggtgc caccgccgcc gccgtgctcg
     1981 ccgctgccgg atggcccgat gcgcgcgagg ccaggctgat cgtcggtcat cagccgacgc
     2041 tcggcgagac cgcggcgctg cttttgtcgg gcagcgacgc cccgtggggc atgcgcaagg
     2101 ctgccgtctg gtggctgtcg aaccggtcgc gagagcaggg cgccgacgtc gtcctcaagg
     2161 ccgtcatcgg ccccgatttt ctctagcgcg gcttcggccc tgccgtggcg cttgcagcaa
     2221 ggcgctactc agcgtgcagg ccctgccact gcgagcgttc ccgctccagc aagtaggcag
     2281 tcagcgggtg cgccgcgagc cagcgcttgc tgacacgtat ggtgacgccg ccccgggtct
     2341 tcagtgccac atgcggcagc gcaacctcgg ttcgggcgtg acagaacagc acggccagcc
     2401 gcaacgccgc gatctgcgcg cgcgccaccg gatcgccgag ctcggcgcgg accttggtca
     2461 ggccgccacg gcagccgtag accagcaacg ccagccgcgc ctgttcgccc gccgaaaacc
     2521 ccggcatgtc ggcgtgctga aggatgtatg cgccgtgctt gtgaaagccg atgtgcgaaa
     2581 ccgacattcc gatctcgtgc agcagcgcgg cccaatggag catctggatc tgctcggggc
     2641 cggccttggg cgcggcgcga cggaacaagg cgcctgcgag cgcgtcgacg cgagtggcat
     2701 gacggcggtc gacctggtag cggtccagaa aacgctcgac agtgagctgt cgagcgtcgc
     2761 ggtccacggt tctgccgagc aggtcgtaga gcacgcctag ccgcagcgcg ccgccgaccg
     2821 gatcgatccg ctcgatgtcg agctcggtga ccgccgcgct catgatggct aagccgcctg
     2881 cgagcaccgg cacgcgctcg ggctttagcg cgttcagctt caggcgtgcc ggatcgcggg
     2941 cgcgaaccag gcgcttcttc aggcgatcga gcccggccgg ggtgatcccg ccgccggaaa
     3001 aaccgttttg ctcgaggatc tcggcgagcg cggccgccgt ccccgacgag gcatacgcac
     3061 ttttccagtg accggcgcca aaatcgccgg cgatggcttc gatttcgacc cgcgccgcgg
     3121 tctccgcggc gtcgagcgcg cccgcagtga ccatcccatc gggaaagaat ttctgcgtgg
     3181 tgctcacaca gcccaaggtc agcgactcga gacgatcggg ctcgagcccg cggccgatga
     3241 taaattcggt cgacccgccc ccgatgtcga cgaccaggcg ctgtgcgtcc gagggaggca
     3301 gctcgtgggc gacgcccaga taaatcagcc gcgcctcttc gtgtccgctg atcacgtcga
     3361 tccgggatcc cagggcgcgc tcggctttgg gcagaaactc gcgcgcgttg gccgcgacgc
     3421 gaaaggcact ggttgcgacc gcgcgcaccg ccgacgggtg gacgcccgac aagcgctcgc
     3481 ggaaccgcgc gaggcaggcg agcgcgacct tgatcgccgc gggcttgatg cgtccatcgc
     3541 ggtcgatacc ggcgccgaag cgcaacgttt cgcgccaggt atcctgccgg tagatctggt
     3601 caccctcgac gcggccgatc tccaggcgga agctgttcga tcctaggtcg acgacggcaa
     3661 gcgtcttggc ggtcacggac tagggcctgt tgacgccggc gtccggcgcg acgagaagca
     3721 gtgcgcacaa gcctaacaga aatacgcgca cgcgaaccaa cgcccgcttt ggacgcacag
     3781 gtgtttctgc tacgctcgct gcctcgtcca cgtcctcgtt tcgccccgtg ccaatcgact
     3841 acctgcaaag aattctgaac gcgaaggtct acgacgtcgc gatcgaatcg ccgctcgagc
     3901 cggcaccgat gctgtcgcgc cgtatcggca atcgcgtact gctcaagcgg gaggataagc
     3961 aggaggtatt ttcgttcaag ctgcgcggcg cctacaaccg gatggcgcat ctttcgagcg
     4021 ccgagcgcgc gcgaggggtc gtcgccgcat cggccggcaa ccacgcccag ggggtcgcgt
     4081 tggccgcgca gcggctaggc tgcaaggcga cgatcgtcat gccggtgacc tccccatcga
     4141 tcaagatcca ggccgtcgag gagcgcggcg caaccgtcgt gctgcacggc gattcctaca
     4201 gcgacgcgta tgcgcacgcg gccaagctgc aaagggcgcg gcgggccacg ttcattcatc
     4261 catacgacga tcccgatgtg atcgccggcc agggcacgat agccgtcgaa atcctgcgcc
     4321 agtgccaggg accgctggac gcgatcttcg tcgccgtcgg cggaggcggg ttgatcgccg
     4381 gcatcgcggc atacgtcaag cgggttcgcc cggaagtcag agtcattggc gtgcagccgg
     4441 tcgactccga cgcgatgacc cggtcgctcg ccgccgggcg ccgcatccgc ctgggacatg
     4501 tcggcctttt cgccgacggc gtcgcggtca agcaagtcgg caaggaaacg tttcgcatct
     4561 gcagggagct cgtcgacgac atgatcaccg tcgacaccga tgcgatctgc gcggcgctca
     4621 aggacgtgtt cgaggacacg cgaacggtgc tcgagccggc cggcgcactg tcggtcgccg
     4681 gcgtgaaggc ctggtgcgag cggcatcggc cgaagcgccg gactttcgtc gccatcgcct
     4741 gcggcgccaa catgaatttc gatcgcctgc gctttgtcgc cgagcgggcc gagctgggcg
     4801 aagcgcgaga agcgatcttc gcggtgacga taccggagcg gccgggcagc tttcgcgagt
     4861 tctgtgcgct gctgggcaag cgcagcgtca ccgaattcaa ctatcgctat gccgatccgc
     4921 atgtcgccca catttttgtc ggcgtcgaag tcgccaatcg tcacgagacc gcggcgctgt
     4981 tgcgcgcgat gcatgcgcgg cggatcgagg cgtacgacct gtccgacaac gaaatggcca
     5041 agctgcatgt gcgccatctg gtcggcgggc atgcgccggc ggcggagaac gaaatcctgt
     5101 accgcttcga gttccccgag cggccgggcg cgctgatgcg gttcctgaac agcatgagcc
     5161 gcggctggaa catcagcctg tttcattacc gcaaccatgg cgccgaccac gggcgggtgc
     5221 tggtcggaat gcaagtgccc ccggcggatc acgccaactt ccgcggcttc ctcaagcgtc
     5281 tcggctatta cggcgtcgac gagacggcca atccggccta ccggatgttt ctcgggcgct
     5341 agcgctgtac gcgtagcccg ccccagccgg caggtcaggc ccgcacaggc cccgtcggcc
     5401 ggggttgcca caccgccagc cctgttttta cggcctccga acggggcttt cacgctgtcc
     5461 gcaagcatga aatcgcgcgc cgggacacgt ccccggaatc gccgcttgac cgcccgcgcc
     5521 cgtgcgatag tgacccgcta cggcagattt ttgcccaatt tcgctcccac gatgttgcaa
     5581 accgctcaaa aacctaaggt ttgtcagttc tggcaaggat tatgctctag gcatttggtc
     5641 ctatacgtgg caaactttcg agggctccaa atggaaacaa cgctggatgc gggattgagc
     5701 ctcaacgcgg gaggaactga attgcgggat ccagtcgccc tgacgggagc gaaggtcatg
     5761 gtgatcgacg atagcaatac gattcgtcgc agcgccgaga ttttcctgct ccaggcggga
     5821 tgcacggtga ttctcgccga agacggcttc gatgcgctgg ccaagatcac cgaccaccag
     5881 ccggacctga tcttcgtcga catcatgatg ccgcggctgg acggctacca gacctgcgcg
     5941 ctgatcaaga agaattcgcg ctttcaccag acgccggtgg tgatgctctc gtcgaaggac
     6001 gggctgttcg accgggcgcg cggcagaatg gtcgggtccg atcagtacct gaccaagccg
     6061 tttaccaagg aaagcctgtt gaaggcggtc ggcacgcacg tcaagtcggc ggcgcattga
     6121 gcgacgcgcc cattcgggcg tgaagaaggg aagcaagggg tgcggaaacg atgaccataa
     6181 aacgcgtatt gatcgtcgat gactcgccga ccgagcggca cgtcctcaac gacatgctga
     6241 ccaaggcagg ctacgaggtc gtcgccagcg acaacggcga ggacgcgatc ctcaaggcga
     6301 agacggtgaa gcccgacctg atactgatgg acgtcgtgat gcccggactc aacggattcc
     6361 aggcgacgcg cgcgatcagc cgcgatccgg cgacgcgcag cgtccccatc atcctttgca
     6421 cgtccaagac ccaggaaacc gacaagatct ggggcatgcg ccagggcgcg cgcgactaca
     6481 tcgtcaagcc ggtgaaccgc gacgagttga tcgccaagat cacggcgctc ggctgatcca
     6541 ctgcaatggc ccgcgccgcc aagctcgacc ttcgcgcgtt ccagcaggaa ctggcggcgc
     6601 gcctcgcggc gaaaaccacg cagcaggtcg agcagtcccg tctgggtctg gcctgcgccg
     6661 gaaagcagtg gctgatccgg ctggcggatg ccggcgaggt catcgcggtg ccgacgatgg
     6721 cgacggtgcc gctgacccgc tcgtggtatc tcggtatttc gaatattcgc ggcaatctgt
     6781 acagcgtgat cgacttcgcc ggattcctcg gcgaggcgat cgagccgctc actctgggcg
     6841 cggcgcaggc gcggctggtg ctcttcgggc cccgcgtcgg cgaattgcgc gctggattgg
     6901 tggtgacgcg ggtactgggt ttgagcaatc tcgccgaatt ggtgcgcagc gacgctgccg
     6961 ccgatgcgcc ggcgtggtac ggcgcgcgct ggaccgacaa ggccggcggc gtgtggcagg
     7021 aaatcgatct ggccctgctg gcgcaggacc cgggctttct tcaagtggga ctgtagcttc
     7081 ggcacagtct gacggattca cgcaggcgta acgaccctat ctgggggagg aatcatggca
     7141 ttcacattca aaggcctctt gggagcaggt gcgggcaggg gacagatcct gtccgaagcc
     7201 gacctcgaag agccgacgac gcaggtcaag atgagcggcg ctcagcgcgg ct
//
LOCUS       DATANS010000127         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_48546, whole genome shotgun sequence.
ACCESSION   DATANS010000127 DATANS010000000
VERSION     DATANS010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48546"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VHT22_01890"
     CDS             <1..718
                     /locus_tag="VHT22_01890"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX3136332.1"
                     /translation="GYTICMMTVAQSISPSIYPKLGFDPLKDFAPVTLMATLPSLLMV
                     HPSLPVKNVKELVALAKAKPATLTYASTGNGTSPHMLMEMFKWMAGGINLVHVPYKGA
                     SPAMIDQISGQIDVAFSTAIAALPFVQQGKVRAIAVSTKDRFAPLPDLPTVEQGGIKG
                     YDGSSWQGVVMPANTPRDMIAKANAELVKMLKSQDMKEKILLQGGIAIGNSPDEFAAY
                     MKSEIDKWAKVAKAAKVRVE"
     gene            676..1335
                     /locus_tag="VHT22_01895"
     CDS             676..1335
                     /locus_tag="VHT22_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136333.1"
                     /translation="MGESGEGRQSESGIDIPRTIGAIHVAPLASGIALIFPALRAAGS
                     ALMLALFGVACSIIGLAAIAGLVRSGDTAVASMLALAFAGVFALPLLLLGQLFIVIAV
                     WTAANSFCVEVTSGGLKVVRNLFGYTVARRAVAGDDITAIDSRMAARYVGAFGPAHYY
                     RLFARSRNSPRPLLIADSLRGPAMTEEIRQLVIGHLARPALAAAGDHAHLEKEEAHDS
                     G"
     gene            1322..>1572
                     /locus_tag="VHT22_01900"
     CDS             1322..>1572
                     /locus_tag="VHT22_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX3136334.1"
                     /translation="MTPDDDTYDERAARIDLVCALRSAERLGLAEGVCNHCSLAVPGR
                     PGQFLINPQGLHWSEVVPDDLVIVDAAGNHVAGKHAVEPT"
BASE COUNT          282 a          508 c          503 g          279 t
ORIGIN      
        1 cggctatacg atctgcatga tgaccgtggc gcaaagcatc tcgccgtcga tttatccgaa
       61 actcggtttc gatccgctca aggatttcgc gccggtcacg ctgatggcga cgctgcccag
      121 cctgctaatg gtccatccgt cgctgccggt aaagaacgtg aaggaactgg tggcgctcgc
      181 gaaggcgaaa ccggcaacgc tcacatatgc atcgaccggc aacggcacga gcccccacat
      241 gctgatggaa atgttcaagt ggatggccgg cggcatcaat ctcgtccacg taccctataa
      301 aggcgcctcc cccgcgatga tcgatcagat ttcgggccag atcgatgtcg cgttcagcac
      361 cgcgattgcc gcgctgcctt tcgtgcagca ggggaaagtg cgcgcgatcg ccgtttcgac
      421 gaaagaccgc tttgcgccgc tgcccgacct gccgactgtc gaacagggtg gcatcaaggg
      481 ctacgacggc agctcgtggc agggcgtcgt gatgcccgcc aacaccccgc gcgatatgat
      541 cgccaaggcc aacgcagagc tggtcaaaat gctgaagtcg caggacatga aagaaaaaat
      601 tctgctgcag ggcggcatcg ccatcggcaa ctcgcccgac gaattcgcgg cctacatgaa
      661 aagcgagatc gacaaatggg cgaaagtggc gaaggccgcc aaagtgagag tggaatagat
      721 atcccgcgca cgatcggcgc gatccacgtc gcgccgctcg cttccggcat cgcgctgatt
      781 ttcccggcac tgcgcgccgc gggctcggcg ttgatgctcg cgctgttcgg tgtcgcgtgc
      841 agcatcatcg ggctcgccgc gatcgcaggt ctggtgcgct ccggcgacac tgcggtggcg
      901 agcatgctgg cgctcgcgtt cgcgggcgtg ttcgcgctgc cgctgctgct gctaggccag
      961 ctcttcatcg tgatcgccgt gtggaccgcg gccaattcct tctgcgtcga ggtgaccagc
     1021 ggcggcctca aggtggtccg taacctgttc ggctataccg tcgcgcgccg cgcggtagcg
     1081 ggcgacgaca tcacggcgat cgacagcagg atggcggcgc gctatgtcgg cgccttcggt
     1141 cctgcgcact actatcgtct tttcgcgcgc tcgcgcaatt ccccgcgccc gctgctgatc
     1201 gccgacagcc tgcgcgggcc ggcgatgacc gaggaaatcc gtcagctggt catcgggcat
     1261 ctcgcgcggc ccgcgctcgc cgcggccggt gatcacgcgc acctggaaaa ggaggaagcc
     1321 catgactccg gatgacgaca cctatgatga acgcgccgcg cgcatcgacc tcgtctgcgc
     1381 gttgcgttcg gccgagcggc tcggcctcgc cgagggcgtg tgcaatcatt gcagccttgc
     1441 ggtgcccggc cggccaggac agttcctgat caatccgcaa ggcctgcact ggtcggaagt
     1501 cgtgccggac gatttggtca tcgtcgacgc ggccggcaat cacgtcgccg gcaaacatgc
     1561 ggtggagccg ac
//
LOCUS       DATANS010000128         1910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_384224, whole genome shotgun sequence.
ACCESSION   DATANS010000128 DATANS010000000
VERSION     DATANS010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384224"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VHT22_01905"
     CDS             <1..888
                     /locus_tag="VHT22_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3136335.1"
                     /translation="IADSVDATIDELRRLVTGITTAANQVTSATGEAQKITGQLLQAA
                     QKQSGEIRDTSKAVTSITKSIHEVSANAAESARVAENSLAAAGKGALAVQNAIYGMND
                     IREQIQETAKRIKRLGESSQEIGEIVQLISDITEQTNVLALNAAIQAASAGEAGRGFT
                     VVAEEVQRLAERSAEATKHIGAIVRSIQSDTQDTVVAMERSTQGVVEGTKVADTAGKA
                     LEEIGVVSRQLATLIASISSATISQTEAAAQVSARMQDILNITNLTTEGTGKTAASTQ
                     QLASLAQELKLSVAGFKLA"
     gene            895..>1910
                     /locus_tag="VHT22_01910"
     CDS             895..>1910
                     /locus_tag="VHT22_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136336.1"
                     /translation="MATRSDVTIDLLSWVKAEIDHALKLVRERTASFLANSENPAALH
                     GCPLELHQVHGALVMVGLEGAARVCAVIERAYQAVIDGRHASKATASTMDRVVYALGQ
                     FLDDLAKGEPNVPLKLFSLYRDLVQLLGEPAAAEIDLFFPDVSVRSPSHENIALAPVD
                     LARQLRTQRAQFQRGMLAWLRDTGSERGLQQMQIAMDCIDRIAQQLSTPRGLWWTAVG
                     FIDALIFTKDAAWLAAAKPLVNRLDTLMRDLANDIEMRDAAVFRELLYAVATCDISTR
                     RIGEIKQLYRLDSQFPEPAVAGLLEYDLESLQPALNDMRARLDVAKSAWVQYTSGDPK
                     GLAL"
BASE COUNT          383 a          623 c          601 g          303 t
ORIGIN      
        1 attgccgatt cggtcgatgc gacgatcgac gagttgcgac gcctggtgac cggcatcacc
       61 accgccgcga accaggttac gagcgcgacc ggcgaagcgc agaaaattac cggccagctg
      121 ctgcaggcgg cgcaaaagca atccggcgaa atccgcgaca cgagcaaggc cgtcaccagc
      181 atcacgaaat cgatccacga ggtatccgcc aacgccgcgg agtcggcgcg cgtcgccgaa
      241 aactcgctgg ccgctgccgg caagggcgcg ctggccgtgc aaaacgcgat ttacggcatg
      301 aacgacatcc gcgagcagat ccaggaaacc gccaagcgca tcaagcggct cggcgaaagt
      361 tcgcaggaga tcggtgaaat cgtgcaattg atctcggaca tcaccgagca gaccaacgtg
      421 ctcgcgctga acgctgcaat ccaggccgcg tccgcgggtg aagcgggccg cggcttcacg
      481 gtggtcgcgg aagaggtgca gcgcctggcg gaacgctccg cggaagcgac caagcacatc
      541 ggcgcgatcg ttcgcagcat tcagtcggac acccaggata ccgtcgtcgc aatggagcgc
      601 agcacccagg gcgtggtcga gggcaccaaa gtagcagaca ccgccggcaa ggcgctggaa
      661 gaaatcggcg tggtgtcacg ccagctcgca acgctgatcg cctcaatctc gagcgccacg
      721 atttcccaga ccgaggcggc cgcgcaggtg tccgcgcgca tgcaggacat tctcaacatc
      781 accaacctca ccaccgaggg tacgggcaaa actgcggcat cgacccagca actcgcgtcg
      841 ctggcgcagg aactcaaact ctcggtcgcg ggcttcaagc tggcctgacg cgtcatggca
      901 acgcgatccg acgtgacgat agacctgttg tcgtgggtca aagcggagat cgatcacgcg
      961 ctgaagctcg tgcgggaacg caccgcgtca ttcctcgcca acagcgaaaa tcccgcggcg
     1021 ctgcacggct gcccgctcga gctgcaccag gtgcatggtg cgctggtcat ggttggcctc
     1081 gaaggcgctg cgcgcgtctg cgccgtcatc gagcgcgcgt accaggcggt aattgacggc
     1141 cgccacgcca gcaaggcgac cgcatcgacg atggatcgcg tcgtctacgc gctgggtcaa
     1201 tttctcgatg acctcgccaa gggcgaacct aacgtcccgc tgaagctctt ttcgctgtat
     1261 cgcgatctcg tgcaactgct cggcgagccc gcggccgccg aaatcgattt gttttttcct
     1321 gatgtgtcgg tgcgctctcc atcgcatgag aacatcgcgc tggcgccggt cgatttggcg
     1381 cgccagttgc gcacccagcg cgcgcaattc cagcggggca tgctcgcatg gctgcgcgat
     1441 accggcagcg agcgcggact acagcagatg cagatcgcga tggactgcat cgatcgcatc
     1501 gcgcaacagt tgtcgactcc gcgcggcctg tggtggaccg cagtcggctt catcgatgcg
     1561 cttattttca ccaaggacgc ggcgtggctg gccgccgcga aaccattggt caaccggctc
     1621 gacacgctga tgcgcgatct cgccaacgac attgaaatgc gggacgccgc ggtgtttcgc
     1681 gagctgctct acgccgtcgc cacctgcgat atctcgaccc gccgcatcgg tgaaatcaag
     1741 cagctttatc gcctcgacag ccagttcccg gagcccgcgg tggcgggcct cctggaatac
     1801 gacctcgaaa gcctgcagcc ggcgttgaac gacatgcgcg cgcggctgga cgtcgccaaa
     1861 agcgcgtggg tccaatacac gtcgggcgac ccgaaggggc tcgcgctgtt
//
LOCUS       DATANS010000129         2148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_720271, whole genome shotgun sequence.
ACCESSION   DATANS010000129 DATANS010000000
VERSION     DATANS010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720271"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1009)
                     /locus_tag="VHT22_01915"
                     /pseudo
     CDS             complement(1..1009)
                     /locus_tag="VHT22_01915"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            1008..>2148
                     /locus_tag="VHT22_01920"
     CDS             1008..>2148
                     /locus_tag="VHT22_01920"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEX3136337.1"
                     /translation="MQIYLVGGAVRDELLGLPARERDWVVVGARPEDLLARGFKPVGK
                     DFPVFLHPQTSEEYALARTERKTGPGYRGFETLFSPDVTLEQDLERRDLTINAIAKDP
                     DGGALIDPFNGQRDLRERTLRHVSPAFVEDPVRVLRVARFAARFAALGFKVAPETLDL
                     MREIAARGELEALVSERVWQETQRALELPAPARFFEVLREANALPVIFPELQALFGVP
                     QPERWHPEIDSGIHTLMVLEQAAKLSDDPVVRFAALTHDLGKGTTPPSEWPSHIAHEQ
                     RGVGLVEGLCDRLRIPNAFRELAVLVSRYHLDAHRVTELRDSTLLDLLERLDAFRRAP
                     RFEQWVLACEADARGRKGLENRDYPQADHLRRARDVAAKVTLDPAE"
BASE COUNT          353 a          718 c          710 g          367 t
ORIGIN      
        1 ccatgcgcgc gagccgtttc gcttcgtcgt agaagcgcgg cagatcgccg tccagctcgc
       61 tcagcacgcg ctgcagtcca ggcacgcacc cgtaatacgt cgcggctgag acgaggtgtg
      121 cgttggtcag cgtgcgctcg aaccagcggt cgtagccggc atacccgttc cattgctgct
      181 gcttgagctg cgtgtactcg agcttcagct cgccgaattc gtattgcttg cggtcgcgct
      241 ttgcgtcgac gggcaacgcc gcttggtaca gctcgcgcag cttggcccgt gccttcaaca
      301 ggagcgcgat gaaatcggac tgctgctcgc gttgttcgcg ccagaccacc agttcctgtt
      361 cgctgtggcg cgtcgccagt cagcgctcca ggccggcttc ctcgacgacc gtggcaaacg
      421 actcgttgaa ctcggaatcg cccggtacgt agacgacctg gtgcgccagc tcatggaaca
      481 acgtgccggc gagctgcgcg tcgctccagc cgagcatcgt gctcagcacg gggtccttga
      541 agtgtcccag cgtcgaatag gctgcaacgc caccgactgc gacgtcgtcg ccgcggcggc
      601 ggagcttgcg cgcgtagtct tgtgcggcgt cctcgctgaa atacccgcgg tagacgacgc
      661 agcccacgat cggaaagcac cagcgacgcg gctcgaccga gaactccggc gcggcgaaga
      721 cgttccagac aacgtaggga cgcccgagat ccgcatagct gcgatacgag tcgttgtccg
      781 gcaggccgag ctcgcgcgag gcgaagtcgc gggcggcggc cacgtattcc agccgggtgc
      841 gcaacttcgg cggcgttgcc gaatccgcca gcacctcggt aatcggttca cgcttcgagg
      901 cgatctccat ctggccgccc gccgcctgca tcagataaca gccggtcagc ggcagcagcg
      961 cggcggcaat cgtcagcact cgggcaattc gatacagtga catggtcatg caaatctatc
     1021 tcgtcggcgg cgctgtgcgc gatgagctgc tcggtctgcc cgcgcgggag cgggactggg
     1081 tggtcgtcgg cgcgcgcccc gaagatttgc tggcacgcgg cttcaagccg gtcggcaagg
     1141 attttccggt gttcctgcat ccgcagacca gcgaggaata cgcgctcgcc cgcaccgagc
     1201 gcaagaccgg tcccggttat cgtggcttcg agacgctgtt ctcgcccgat gtgacgctgg
     1261 agcaggatct ggaacgtcgc gatctgacga tcaacgcgat cgcgaaggat cccgacggcg
     1321 gcgcgctgat cgatccgttc aacggccagc gcgatctgcg cgagcgcacg ttgcgacacg
     1381 tctcgccagc tttcgtcgaa gatccggtgc gcgtgctgcg cgtcgcgcgc ttcgccgcgc
     1441 gctttgcggc gctcggcttc aaggtcgcac cggaaacttt ggacctgatg cgcgagatcg
     1501 ctgcacgcgg cgagctcgaa gcgctggtca gcgagcgcgt gtggcaggag acgcaacgcg
     1561 cgctcgagct gcccgcgccc gctcgattct tcgaagtgct gcgcgaggcg aacgcacttc
     1621 ccgtcatctt tcctgagctg caggccctgt tcggcgtacc gcaacccgag cgctggcacc
     1681 ccgagatcga ctccggcatc cacacgctga tggtgctgga gcaggccgcg aaactgagcg
     1741 acgacccggt cgtgcgcttc gcggcgctga cgcacgacct cggcaaaggc acgaccccgc
     1801 cgagcgagtg gccgagtcac atcgcgcacg agcagcgcgg cgtcgggctc gtcgaaggat
     1861 tgtgcgatcg gctgcggatt cccaacgcgt ttcgcgagct cgcggtgctc gtatcgcgct
     1921 atcacctcga tgcgcatcgc gtcaccgagc tgcgcgacag cacgctgctc gacctgctcg
     1981 aacgcctcga cgcattccgg cgtgcgccgc gtttcgagca gtgggtgctc gcatgcgaag
     2041 cggacgcgcg cggtcgcaag ggactggaga atcgcgacta cccgcaggcg gaccatctgc
     2101 gtcgagcccg cgacgttgcg gcaaaggtca cgctggatcc ggccgagc
//
LOCUS       DATANS010000130         4918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_677830, whole genome shotgun sequence.
ACCESSION   DATANS010000130 DATANS010000000
VERSION     DATANS010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677830"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..888)
                     /locus_tag="VHT22_01925"
     CDS             complement(247..888)
                     /locus_tag="VHT22_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX3136338.1"
                     /translation="MNKQLLRFAIVGTLIGTALPLYAQEGFYGGVSLRQGGADAAGVA
                     LGGPGLVWNRFVAPAAEDAAQQTLLFGGYRWKSDISVEAAFNSRDQYALKAGSDRPAG
                     VGLGLVDTSTRAWNADVYTSWEFLHSFSLYGRLGYAQNDSRPLFSGASLVPGDPRRFR
                     DGVNYGVGLRYILSQALGLRVEYSRFGRFAGESSSVSVLPESDQVTIGVQYRF"
     gene            complement(1088..1597)
                     /gene="mobB"
                     /locus_tag="VHT22_01930"
     CDS             complement(1088..1597)
                     /gene="mobB"
                     /locus_tag="VHT22_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEX3136339.1"
                     /translation="MRVFGFAGWSGSGKTTLIEQLIPRFGSLGLTVSLIKHAHHEFDV
                     DQPGKDSYRHRAAGCQEVLVSSAARWAQMHELRGAPELSLAQAVQRLSPCDLVLVEGF
                     KAAAIPKLEVYRASVGKPLLHPGDPHIVAIATDATLGTSLPHFDIVDVAGIADFIAAC
                     RLPAPAAWN"
     gene            complement(1659..2771)
                     /locus_tag="VHT22_01935"
     CDS             complement(1659..2771)
                     /locus_tag="VHT22_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX3136340.1"
                     /translation="MRIGVPRERKDGECRVALTPDGARSLVAAGHQIVVERGAGERVG
                     FSDAAYVAAGARIAADAASAFDCPLVVKVKELQRDEIAVLKPATTVMGFAQLARDREL
                     LDAVLAARIGLIGYEAVGDGRGGLPLLAPMSRIAGRLAPLIAASLLMNDSGGAGVLLP
                     GVDAIAPARIVIVGAGNVGSQASQVALALGASVTVFARKPARLGLLRARCEGVAGERL
                     STALSDPQTLAAAIAEADVLIGAVLEPGRLSPKLVSRAMLRAMRAGSVFIDVGIDHGG
                     IGETSRMTSISAPTFVAEGVLHYGVANMPALVARTATLALAQAALPCVHRLADRGVAA
                     ALRSDAGLRAGLQVWDGAIVHPGLAADVGLLVAPWQ"
     gene            complement(2784..3317)
                     /locus_tag="VHT22_01940"
     CDS             complement(2784..3317)
                     /locus_tag="VHT22_01940"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEX3136341.1"
                     /translation="MSPILPYAILPPLYVEYDASAPDVAGRVIELVGAAAPWARIDHI
                     GSTAIPGCAGKGIIDLVALYPEGRLEAMRDTIDALGFQPQRTGHAFPAERPMRVGAIE
                     HRGHVYRLHMHVVAADSDEAQSLYRFRDVLRSDPALRDAYQAKKRAILQTGVESSNDY
                     THAKGEFISAVLSRSLH"
     gene            3436..4029
                     /locus_tag="VHT22_01945"
     CDS             3436..4029
                     /locus_tag="VHT22_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HEX3136342.1"
                     /translation="MTGLPSVAPLAGSVADAIENIVASIPAPREAASNEPGSAARALA
                     RKCATRAAALSGALALPPGLFGMLTVLPDLIAIWRIQAQMVSDIAGLYGKEVELTRAH
                     MVYCLFRHATSQVARDVVVRAGERMVIRQVSGGALKSVLASVGMSVTQRVAGTAASRW
                     LPVIGAAAVAGYAYFDTLQVARTAISLLETRDLAIPA"
     gene            complement(4073..>4918)
                     /locus_tag="VHT22_01950"
     CDS             complement(4073..>4918)
                     /locus_tag="VHT22_01950"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX3136343.1"
                     /translation="KVQDGRLDLPLQASVFGGTVIANLSLDAARQPTPVLALHAQMKG
                     MDLGALLAAVGARREVRGGKTDGRIDIGATGNTPRQWESTMSGTVIALVGPATLVNTK
                     VDLSSPLNQLGATVNPFHNVDPSTELQCAVIRLSLHNGFAEVERSIAVETKKFGATAS
                     GTLDFRTRTLDLAIRPQLRQGVPVDIPQVAELVRFRGPFASPTVAIDSVAAAATVARI
                     GAAAYTGGLSIVGESLLSRPGGNPCEIALGRTSGAAAQIAGGGGGAGTVVEDMGKALG
                     RAFHR"
BASE COUNT          826 a         1683 c         1642 g          767 t
ORIGIN      
        1 cacggactgc gcgcccgggt gccagctcgg acgaccacgc gcccccgcgg ctgcgcagcc
       61 acgcgaggag cgcgccgaca tcggcgacgt cgccgggcag cgcgacccgt tcgccggccg
      121 tgccgaagcg gtcccgcagg cgggcaagat agacgatctc aaccatgacg gaatcttagc
      181 gcatcggcgg cgcggcaaaa cgaaaacgcc ggcgcgaggg gcgccggcgt cgcttgaatc
      241 cggcagttag aagcggtact ggacgccgat agtgacctga tcggattcgg gaaggacgct
      301 gacgctgctc gactcaccgg caaaccggcc aaatcgcgaa tactcgacgc gaagtcccag
      361 cgcttggctc agtatgtagc gcaagccgac gccgtagttg acgccgtcgc ggaaccgccg
      421 cgggtcgccc ggcaccagcg aggcgccgct gaacagcggg cgcgaatcgt tttgcgcata
      481 gcctaggcgt ccgtacaacg aaaagctgtg caggaattcc caactggtat agacatcggc
      541 gttccaagcc cgagtcgacg tgtcgaccag ccccagaccc acgcccgccg ggcggtcgga
      601 gccggccttg agggcatact ggtcgcggct attgaatgcg gcctcgaccg aaatatcgct
      661 cttccagcgg tacccgccga agagcagcgt ctgctgggcg gcgtcctcag ccgctggggc
      721 gacgaaccgg ttccagacga gccctggccc gcccagggcc acacccgcgg catccgcgcc
      781 gccttggcgc agcgagacgc cgccgtagaa cccctcctgg gcgtacagag gcagcgccgt
      841 cccgatcagc gtcccgacga tcgcgaacct aagtagctgt ttgttcacgt attttatgcc
      901 gttgtcccgc cgctgtacct cagcagtcca ccgggatgtc cacagcttca acttaccgct
      961 cgggcgagcc ggtgtcaatt tccatcgtcg ctgtcggccc ggggtgttcc cccagtgttg
     1021 caggtgcgcc aaaggcgcac gtacgttcgg tcagactgct cacccgttcg acgcccgccg
     1081 gaaggggcta gttccaggcg gccggggccg ggagccggca cgcggcgata aagtccgcga
     1141 ttcccgccac gtcgacgata tcgaaatgtg gcaaggatgt gcccagcgtc gcgtcggtgg
     1201 cgatcgcgac gatgtgcggg tcgcctggat ggagcaacgg cttgccgacg ctcgcccggt
     1261 aaacctcgag cttagggatg gcggcggcct tgaacccctc gaccagcacc agatcgcagg
     1321 gcgacagccg ctgcacggcc tgcgcgagcg agagctccgg tgcgccgcgc aattcgtgca
     1381 tctgggccca gcgcgccgct gacgacacca gcacttcctg acatcccgcc gcgcgatggc
     1441 ggtacgagtc cttgcccggt tggtcgacgt cgaattcgtg atgcgcatgc ttgatcagcg
     1501 agacggtaag accgagcgaa ccgaaacgcg gaatcaactg ctcgatcagc gtcgtcttgc
     1561 cgctgccgga ccagccggcg aatccgaata cccgcatgac gtttcccaaa ttccttgcag
     1621 cgctgtcttg tactggagtt cgttggcgcg ggcaaggatt attgccacgg ggcaacgagc
     1681 aacccgacgt cggcggcaag ccctgggtga acaatcgcgc cgtcccagac ttgcaggccg
     1741 gcgcgcaagc ccgcgtcgct gcgaagcgcg gccgcgacgc cacgatcggc aagccgatgc
     1801 acgcacggaa gcgccgcttg tgccagcgcc agcgtcgcgg tgcgcgcgac cagcgccggc
     1861 atgttcgcca cgccgtaatg caggacgcct tcagcgacga aggtcggcgc cgaaatcgag
     1921 gtcatgcgcg aagtctcgcc gatgccgccg tgatcgatcc cgacgtcgat gaagaccgag
     1981 ccggcccgca tggctcgcag catcgcgcgc gataccagct tcggcgaaag ccggccgggc
     2041 tcgagcaccg cgccgatcaa cacgtcggct tccgcgatcg ccgcggcgag cgtttgcgga
     2101 tcgcttagcg ccgtcgacag ccgctctccg gcgacgcctt cgcaacgcgc gcgcagcagg
     2161 ccgagccgcg ccggcttgcg cgcgaacacg gtgacgcttg cgcccagcgc gagcgctact
     2221 tgcgacgctt ggctgccgac gttgccggcg ccgacgatga cgattcgggc cggagcgatc
     2281 gcgtcgacgc ccggcagcag tacgcccgcg ccaccgctat cgttcatcag cagcgatgcc
     2341 gcgatcagcg gcgcgagccg gcctgcgatg cgcgacatcg gcgcaagcag cgggagtccg
     2401 ccacgaccgt cgccgacggc ctcataaccg atcaggccga tgcgcgccgc gagcaccgcg
     2461 tcgagcagct cgcgatcgcg cgccagctgc gcgaatccca ttaccgtcgt cgccggcttg
     2521 agcaccgcaa tctcgtcgcg ctgcagctcc ttgaccttga cgaccaacgg gcaatcgaaa
     2581 gcagacgcgg cgtctgcggc tatgcgcgcg cctgccgcga cataggccgc atcgctgaag
     2641 cccaccctct cgccggcgcc gcgctcgacc acgatctgat ggcccgcggc gaccagcgat
     2701 cgagcgccgt cgggtgtcag ggcgacgcgg cattcgccgt ccttgcgctc gcgcgggacg
     2761 ccgatacgca ccggtccgcc ccgtcagtgc aggctgcggc tcaacaccgc gctgatgaat
     2821 tcgcccttgg cgtgcgtata gtcgttcgag ctttcgacac ctgtctggag aatcgcgcgt
     2881 ttctttgcct ggtaggcatc gcgcagcgcc ggatcggagc gaaggacatc gcgaaagcga
     2941 tagagagact gtgcctcgtc cgaatcggcc gcgaccacgt gcatgtgcag ccgatacacg
     3001 tgaccgcggt gctcgatcgc accgacacgc atcggccgtt cagcggggaa cgcatggccg
     3061 gtacgctgcg gttgaaaacc cagggcatcg atcgtgtcgc gcatggcctc cagcctgccc
     3121 tcgggataaa gcgcaacgag gtcgataatg cccttgccgg cgcaacccgg gatcgcggtg
     3181 ctgccgatat ggtcgatgcg cgcccaaggc gccgcggctc cgaccaattc gatgacgcgc
     3241 cccgcgacat cgggtgcgct ggcgtcgtat tcgacgtaca gaggcggtag gatcgcgtac
     3301 ggcagtatcg gcgacatcgc ggacgctcgg ctgcttttcc tgcgggcgat tgtaaccttt
     3361 atactcgggt tgtctctcgc cccggttgtg gcggtgcggc aatccggccg cgggcgagag
     3421 tcgaggaggt cgtcaatgac cggtttaccg tcggtggccc cgctcgccgg ctcggtggcc
     3481 gacgccatcg agaacatcgt cgccagcatt ccagcgccgc gcgaggcggc gagcaatgaa
     3541 cctggaagcg ccgctcgcgc gctcgcgcgc aagtgcgcga cgagggcggc cgcgctgtcg
     3601 ggggcgctcg cgcttccgcc ggggctgttc ggcatgctga ctgtccttcc cgatctcatc
     3661 gccatctggc gcatccaggc gcagatggtc tccgatatcg cgggcttgta cggcaaggaa
     3721 gtcgagttga cccgggcgca catggtgtac tgcctgttcc ggcacgcgac cagccaggtg
     3781 gcgcgcgacg tggtcgtacg cgccggcgag aggatggtca tacggcaggt gtcgggcgga
     3841 gcgctgaaga gcgtattggc cagcgtcggc atgtcggtga cgcagcgcgt ggcgggtact
     3901 gcggcgagcc gctggctccc ggtaatcggc gcggccgccg ttgcaggtta cgcttatttc
     3961 gacacgctgc aggtggcgag aaccgcgatc agcttgctcg agacgcgcga cctggcgatc
     4021 cccgcctgaa ggcggaaacg cgcgaatcgc aaagccggct gcagggccct cgtcagcgat
     4081 ggaaggcgcg gcccagcgcc ttacccatgt cttcgaccac cgtgcctgcg ccaccgccgc
     4141 caccggcgat ctgcgccgcc gcgcccgatg tccggccgag cgcgatttcg caggggttgc
     4201 cgccggggcg cgacaacagc gactcgccga cgatcgataa cccgccggtg taggcggctg
     4261 cgccgatgcg cgcgacggtg gcggcggcgg ccaccgaatc gatcgcgacc gtgggtgaag
     4321 cgaacggccc gcggaaacga accagctcgg caacctgcgg aatgtccacc gggacgccct
     4381 gccggagttg cggccggatg gcgagatcca gcgtgcgcgt acgaaagtcc agcgtgccgc
     4441 tcgcggtggc gccgaatttt ttggtttcga cggcgatgct ccgctcgact tccgcgaagc
     4501 cgttgtgcaa ggacagccga atcaccgcgc attgcagctc ggtcgacgga tcgacattgt
     4561 gaaacggatt gaccgtggcc ccgagctggt tcagcggcga actcagatcg accttcgtgt
     4621 ttaccagcgt cgccggcccg accagcgcga taaccgtccc gctcattgtg ctttcccact
     4681 gcctcggcgt attaccggtg gcgccgatat cgatccggcc atcggtcttg ccgccccgca
     4741 cctcgcgcct ggcgcctact gctgcgagca gcgcgcccag gtccatccct ttcatctgcg
     4801 cgtgcagcgc caatacaggc gttggctggc gagcggcatc gagcgacaga ttggcgatga
     4861 ccgtcccgcc gaataccgac gcctggagcg ggaggtcgag gcgtccgtcc tggacctt
//
LOCUS       DATANS010000131         1758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_258638, whole genome shotgun sequence.
ACCESSION   DATANS010000131 DATANS010000000
VERSION     DATANS010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258638"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VHT22_01955"
     CDS             <1..251
                     /locus_tag="VHT22_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEX3136344.1"
                     /translation="RYVLTPRIFHHLRTMPPGAGGEIQLTDGIARLLEDEKVLAHEFE
                     GRRYDCGNKLGYLEATVELALKHPDVGPAFADFLKRMR"
     gene            248..1174
                     /locus_tag="VHT22_01960"
     CDS             248..1174
                     /locus_tag="VHT22_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HEX3136345.1"
                     /translation="MTFNELRYIVAVAQERNFGRAAQHCFISQPALSVAIQKLEEELG
                     TQIFERGKSEVTVTPVGERIVEQAQKVLEEAASIRDIAQAGRNQLVGLFRLGVIYTIA
                     PYLLPDLIPALNERAPAMPLEIEENLTENLETSLRAGRIDAAIIALPFNPPGIATDFL
                     YEEPFQVVVPEKHAWARRKSIDPSELAGEHAILLNVGHCFRDQVLDSCPELNRSGAPI
                     TRTNSLETIRNMVASGLGVSVLPRDALIPKYHSRLVVPVPFSRPVPSRRVALAHRKSF
                     PRPAAIAAIRDAVAACRGASGGASPRRGAPRA"
     gene            1316..>1758
                     /locus_tag="VHT22_01965"
     CDS             1316..>1758
                     /locus_tag="VHT22_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3136346.1"
                     /translation="MMATDLKNKVVLITGASTGIGAAAARAFAEQGSKVAVHYNASKE
                     AAEKVAREVKSAGGEALLVGGDVTKSANVKRIVADTLAGFGRIDVLVNNAGGLVQRTK
                     IEDYSEDFLHQVLDLNVIQVAMFMHEVVPAMRKQGGGNIINVSSIA"
BASE COUNT          322 a          583 c          578 g          275 t
ORIGIN      
        1 gccgctatgt gctgacgccg cgcatcttcc accatctgcg tacgatgccg cccggcgccg
       61 gcggcgagat ccagcttacc gacgggatcg cgcggctgct cgaggacgag aaggtgctgg
      121 cgcacgaatt cgaaggccgc cgctacgact gcggcaacaa gctcggctac ctggaggcaa
      181 cggtcgagct cgcgctgaag catcccgatg tcggccccgc ctttgccgac ttcctcaagc
      241 gcatgcgatg acgttcaacg agctgcgtta catcgtcgcc gttgcgcagg aacgcaattt
      301 cggccgggcg gcgcaacatt gcttcatcag ccagccggcg ctgtcggtag cgatccagaa
      361 gctcgaagag gagctgggca cgcagatttt cgagcgcggc aagagcgagg tgaccgtaac
      421 gccagtcggc gagcgcatcg tcgagcaggc gcagaaggtc ctggaggaag cggccagcat
      481 ccgtgacatc gcgcaggctg gacgtaacca gctggtcggc ctgttccgcc tcggagtgat
      541 ctacacgatc gcgccctacc tgctgccgga ccttattccg gcgctgaacg agcgcgcacc
      601 ggcgatgccg ctcgagatcg aggaaaatct caccgaaaac ctggagacgt cgctgcgggc
      661 cggccggatc gacgccgcga tcatcgcctt gccgttcaac ccgccgggca tcgccactga
      721 cttcctctat gaggaaccgt tccaggtcgt cgtgccggaa aagcacgcgt gggcccgacg
      781 gaagtcgatc gacccatccg agcttgccgg cgagcatgcg atcctgctca atgtcggtca
      841 ttgctttcgc gaccaggtgc tcgattcctg cccggagctc aatcgctccg gcgcaccgat
      901 cacgcgcacc aattcgctcg agacgatccg taacatggtc gcctcggggc tgggcgtgtc
      961 ggtgctgccg cgcgacgcac tgatcccgaa ataccatagc cgcctcgtcg tgccggtgcc
     1021 gttctcgaga ccggtccctt cgcgccgcgt tgcgctcgcg catcgcaaga gctttccgcg
     1081 tcccgccgcg atcgcagcga tccgggacgc ggtcgcggcc tgccgcggcg cgagcggcgg
     1141 cgcgagtccg cggcgagggg ccccgcgcgc gtagcgcgtg gggatcgcgc gaggcgagtc
     1201 gacgccaagt ggccgacgcg aggccttcgc atgcagagat cgtgcggagg ccactcaagg
     1261 cgagggactc gtgcgcgcgt agtacaattt gctgcgctcg accaacgacg gagcaatgat
     1321 ggcgacggat ctgaagaaca aggtcgtatt gatcaccggc gcttccaccg gcatcggcgc
     1381 ggccgccgcc cgcgcgttcg cggagcaagg cagcaaagtg gcggttcact acaacgccag
     1441 caaggaagcc gcggaaaaag tcgcgcgcga ggtcaagtcg gccggcggcg aagcgctgct
     1501 ggtcggtggt gacgtcacca agagcgccaa cgtcaagcgc atcgtcgccg acacgctcgc
     1561 cggcttcggc cggatcgacg tcctggtcaa taacgccggc ggcctggtcc agcggaccaa
     1621 gatcgaggac tacagcgaag acttcctgca ccaggtgctc gacctcaacg tcatccaggt
     1681 ggcgatgttc atgcacgagg tcgttcccgc gatgcgcaag cagggcggcg gcaacatcat
     1741 caacgtcagc tcgatcgc
//
LOCUS       DATANS010000132         1618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_341758, whole genome shotgun sequence.
ACCESSION   DATANS010000132 DATANS010000000
VERSION     DATANS010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341758"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VHT22_01970"
     CDS             complement(<1..358)
                     /locus_tag="VHT22_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003279593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3136347.1"
                     /translation="MNIDRIDHIVLTVRDIDATCAFYSRVLGMDVTTFGAGRKALVFG
                     GQKINLHQAGREFEPKADAPTPGSADFCLISAIPLREAIAHLQSCGVTIVEGPVQKTG
                     ARGPIQSVYFRDPDFNL"
     gene            complement(366..860)
                     /locus_tag="VHT22_01975"
     CDS             complement(366..860)
                     /locus_tag="VHT22_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX3136348.1"
                     /translation="MTGTGAFPLEGGCTCRAIRYQMTSAPLFVHCCHCRWCQRETGAS
                     FALNAMIEADRVVVLKGEPEAVATPSNSGKGQKIIRCPSCRIALWSNYAGAGDAVRFV
                     RVGTLDEPDRLPPDIHIFTASKQPWVVLPPGTPAVAEYYDREKYWPAESLERRRTLLA
                     SKHP"
     gene            complement(857..1204)
                     /locus_tag="VHT22_01980"
     CDS             complement(857..1204)
                     /locus_tag="VHT22_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136349.1"
                     /translation="MPHQTSSSTIETDRLEGLSRELQRWLTGVRASLANEIRSYPTPI
                     PRCDAQFNHLVEQRGRLSRLLTDLEVALDRRDGDPALRAVLAQLPDLPPQGESIEERS
                     LRDRIGAELTRSR"
     gene            complement(1346..>1618)
                     /locus_tag="VHT22_01985"
     CDS             complement(1346..>1618)
                     /locus_tag="VHT22_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX3136350.1"
                     /translation="THLLLYALLLVLPILGWAAASARDWTVRVFDLVTLPHLVPPEAK
                     IGFLAGDVHVVLSYVLLGLIGLHVAAGLYHYFVRHDQVLQRMLAGD"
BASE COUNT          300 a          500 c          544 g          274 t
ORIGIN      
        1 caagattgaa gtcggggtcg cggaaataca cggactggat cggcccgcgt gctccggttt
       61 tctgcactgg cccctcgacg atcgtgacgc cgcagctttg gagatgggcg atggcctccc
      121 gcagcggaat cgccgatatc aggcagaaat cggccgagcc gggcgtcggc gcatcggcct
      181 tgggctcgaa ctcccgaccg gcctggtgca ggttgatctt ctggccgccg aacaccagcg
      241 cctttcgtcc ggcaccgaag gtcgtcacgt ccatgccgag cacgcgtgaa tagaacgcgc
      301 aggtagcgtc gatgtcgcgc accgtcagca cgatgtgatc gatgcgatcg atattcatcg
      361 acgcactaag ggtgcttgct ggccagcagc gtgcgacggc gttcgaggct ctcggccggc
      421 caatatttct cgcgatcgta atactcggcg accgcgggtg tgcccggcgg caacaccacc
      481 catggctgct tcgaagcggt gaagatgtgg atatcggggg gcaggcgatc gggttcgtcg
      541 agcgttccga cccgcacgaa gcgcaccgcg tcgcccgctc cggcgtaatt gctccacagc
      601 gcgatccggc agctggggca gcggatgatc ttctggccct tgccgctgtt cgagggcgtt
      661 gccaccgctt ccggctcgcc cttgaggacc actacgcggt cggcttcgat catcgcattg
      721 agcgcgaatg acgcgcctgt ttcgcgctgg caccagcgac aatggcaaca atggacgaac
      781 agcggcgcgc tggtcatctg gtagcggatc gcgcggcagg tgcagcctcc ttcgagcgga
      841 aatgctccgg tgccggtcat cggcttcgag tcagttcggc gccgatgcga tcgcgcaagc
      901 tgcgctcctc gatcgactcg ccctgcggtg gcagatctgg cagctgcgcc agcacggcac
      961 gcaatgcagg gtcgccatcg cggcgatcga gcgcgacctc gaggtcggtc agcagtcgcg
     1021 acaggcggcc acgctgctcg accaggtgat tgaactgggc gtcgcagcgc ggaatcggcg
     1081 tcggatagct gcgaatctcg ttggcaagcg acgcccggac gccagtgagc cagcgctgaa
     1141 gctcgcgcga caatccttcg agacggtccg tctcgatcgt gctggacgat gtctgatgcg
     1201 gcatggccat attcggctgg acggcggatg cctcgaaagg aagcggcgcg cctgcggcag
     1261 cgcacggcga tatgatgcac ggatttgccg aaaaattgaa ggcatccaca gtggacctgt
     1321 gtccggcctt cgcgcaaagc gaggcttagt cgccggcgag catccgttgc agcacctggt
     1381 cgtgccgcac gaagtaatga tacaggccag cggcgacgtg aagtccgatc aggccgagca
     1441 ggacgtacga cagcacgacg tggacgtcgc ccgcgaggaa cccgattttt gcttcgggcg
     1501 ggactaggtg gggaagggtg acgagatcga atacgcgaac cgtccaatcg cgggccgatg
     1561 ccgccgccca gcccaggatc ggcagaacca gcaacagcgc atacagcaac agatgggt
//
LOCUS       DATANS010000133         2122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_48872, whole genome shotgun sequence.
ACCESSION   DATANS010000133 DATANS010000000
VERSION     DATANS010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48872"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VHT22_01990"
     CDS             complement(<1..391)
                     /locus_tag="VHT22_01990"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3136351.1"
                     /translation="MHSDATNTTLFERWLPSEPCATVLKTDLFDEAMGSGLYPLLSAR
                     GRRVAAIDVAPTVLAAATARYPALLAVEADVRRMPFAGDTFDVVVSNSTLDHFETHDE
                     ILASLRDFHRILRPGGRLLLTMDNLANP"
     gene            747..1742
                     /locus_tag="VHT22_01995"
     CDS             747..1742
                     /locus_tag="VHT22_01995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3136352.1"
                     /translation="MPVYNGSGTLRQSLEPLLAMRRKGEIAEVIVVDDGSNDASAAIA
                     AAAVVTVLESGGRLGPGGARNVGAAAAKSDIRWFVDADVVVHADGARVLVEALQRSDA
                     AAVFGSYDDAPPAPNFFSQYKNLVHRYHHIRARGDIDTFWAGCGAVARQAFVDVGGFD
                     AAKYPHSSIEDIELGLRLRQRGLRIHLEPALLATHLKVWHLPNLLRTEVWRRAIPWSR
                     LIIEHGWWSRNLNMDGSERVRALLAVCWLATLLLAAARVSPPWPALILLGLIAVANRR
                     LFGYFRGRRGMLFAIGGVLYHQVYYLYSVAAFAYCLAAYQWTAVRRRLDRRTHRA"
     gene            complement(1777..>2122)
                     /locus_tag="VHT22_02000"
     CDS             complement(1777..>2122)
                     /locus_tag="VHT22_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136353.1"
                     /translation="EFINVSRPMFEHFFEMDNWHLLWYLTLGVIALRWRAVFSAATAP
                     MTVTMLGAFAFVFVVFFYSSAAFGVADETLVNRLPLHMVPALAFYLAFLWTQAPARTS
                     GVQSAAPRQAVQ"
BASE COUNT          377 a          695 c          692 g          358 t
ORIGIN      
        1 cgggattggc gagattgtcc atcgtaagca acaggcggcc gcctggccgc agtattcgat
       61 gaaaatcgcg cagcgacgcc agtatttcgt catgcgtttc gaagtggtcg agcgtggaat
      121 tcgagacgac gacatcgaag gtgtcgccgg cgaacggcat gcgccggaca tcggcctcga
      181 cggccagcag cgccgggtaa cgcgccgttg ctgcggccag caccgtcggc gcgacatcga
      241 ttgccgcgac gcgccggcca cgcgccgaca acagcggata caacccgctt cccatcgcct
      301 cgtcgaacag atcggtcttg aggacagtgg cgcagggttc cgacggaagc cagcgctcga
      361 acaaggtggt gttggttgca tcgctgtgca aacgccaaag cgctccggca ggttcactta
      421 gcagcgactc gagcgcgctg tcccagcgcc gtgatgcggc ctcaccagag cggatgtcgt
      481 tgccatccgt ggtcgcgcgt accttacgtt cgatcgattg attgattgcg cgggccatga
      541 cgactgatta gactgcgggt gctcgaacgc gcgcgatttg tctcggccta tgccgcgcgg
      601 atcgacgcga ccaagtatag tgctcgcgcg gcaaaagcga ccgccggcgc gatattgcac
      661 agccggccac tgcgccggtc gggggaaacg cactgaacgc agcgaccgaa agcacgagcc
      721 ctgcggcgct cgccgtcacg gtcatcatgc ctgtttacaa cggcagcggc acgctgcggc
      781 aaagcctcga gcctctgctc gcgatgcggc gcaaaggcga gatcgctgaa gtcatcgtag
      841 tcgacgacgg ctcgaacgac gccagcgcgg cgatcgccgc cgccgcagtg gtcaccgtgc
      901 tcgaatccgg cggccgcctc gggcccggcg gcgcgcgcaa cgtcggagcc gccgccgcaa
      961 agagtgacat ccggtggttt gtcgatgccg acgtcgtcgt tcacgccgat ggcgcacggg
     1021 tgctggtcga agcgctgcag cgaagcgacg cggccgcggt cttcggcagc tatgacgatg
     1081 ccccgccggc gcccaatttt ttctcgcagt acaagaatct tgtccaccgc taccaccata
     1141 tccgcgcccg aggcgacatc gacacgttct gggccggctg tggtgcggta gccaggcagg
     1201 ctttcgtcga cgtcggcgga tttgacgccg ccaagtaccc tcattcgtcg atagaggaca
     1261 tcgagctcgg attgcgtttg cggcagcgcg ggcttcgcat ccacctcgag ccggccctgc
     1321 tggcgaccca tctcaaggtc tggcacctgc ccaacctgct gcgcaccgag gtctggcgtc
     1381 gcgcgatacc ttggtcgcgc ctgatcatcg agcacggatg gtggtcgcga aacctgaaca
     1441 tggacggcag cgagcgcgtg cgtgcgctgc ttgccgtctg ctggctggcg accctgctgc
     1501 tcgccgcggc gcgcgtctcg ccgccctggc ccgcgttgat ccttctcggt ctcattgcgg
     1561 tggctaaccg gcggttgttc ggctacttca gggggcggcg cggcatgttg ttcgcgatcg
     1621 gcggcgttct ctatcaccag gtctattacc tgtacagcgt cgcagcgttc gcatattgcc
     1681 tggcggcgta ccaatggacg gcggtacgcc ggcgcctcga ccgacggacg caccgcgcct
     1741 gatcgcgagg gcggcagccg cgcgatcatc gcgccctcat tgcacagcct ggcgcggtgc
     1801 cgcgctttgg acgccggatg tccgcgccgg cgcttgcgtc cacaggaacg ccagatagaa
     1861 cgccagcgcc ggcaccatgt gcaacggcag gcgattgacc agcgtctcgt cggcgacgcc
     1921 gaaagcggcg ctgctataga agaacacgac gaaaacgaaa gcgaacgcac cgagcatcgt
     1981 gacggtcatc ggcgccgtcg cggcggaaaa aaccgcgcgc cagcgcaacg cgatcacccc
     2041 cagcgtcagg taccagagca gatgccagtt gtccatttcg aaaaagtgct cgaacatcgg
     2101 gcgcgacacg ttgatgaatt cg
//
LOCUS       DATANS010000134         1792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_49144, whole genome shotgun sequence.
ACCESSION   DATANS010000134 DATANS010000000
VERSION     DATANS010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49144"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="VHT22_02005"
     CDS             complement(<1..1117)
                     /locus_tag="VHT22_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX3136354.1"
                     /translation="MQRIVKIVGACTFGLSLTAAAADYSMTVNRDRLLNAQNEPQNWL
                     MMNGDYGSLRYSKLTQINRDNVKNLRMVWAMALGGMQDVGANGPESQVNPLIDNGYMY
                     TTDGWGTIYKIDARNPGKGEFVWICDPGVTRKGNVSRTRGIALWEDLVIANLPDGRVI
                     GINRDNGEIVWDKKILVANEFGRKERFFAAPITADGKVIIANGAGDGKTRGWIAALDA
                     RTGNELWRWYAVPAPGEPGSETWKDKNQAWKTGGGGLWQTGSYDPVTRLTIWGTGNPI
                     PQYDPQARAGDNLYTNSAVAINIDTGKLAWYFQYTPNDSWDYDEVGIHMLHDSQVDGK
                     TRKVVTHFARNGFFYTLDRTNGKFIKADQYVNDVNWTK"
     gene            complement(1324..>1792)
                     /locus_tag="VHT22_02010"
     CDS             complement(1324..>1792)
                     /locus_tag="VHT22_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3136355.1"
                     /translation="ITFSPAYVDIDATYLVPAGSQILDVADIDRDGMRVAIAAKSAYE
                     FFLSRNLKHAKLVAGPGTFGAYDVFVSGKLDALVGIRPRLVIESEKLPGSRVLAGRFM
                     AIEQTIATPKDRVAAARYVRDFIEDVKASGAVARMLADHAVRGVSVSPPARSD"
BASE COUNT          287 a          558 c          574 g          373 t
ORIGIN      
        1 ccttggtcca gttaacgtcg tttacgtact ggtcggcctt gatgaattta ccgttggtgc
       61 ggtcgagcgt gtagaaaaac ccgttgcgcg cgaaatgcgt gacgaccttg cgcgtcttgc
      121 cgtcgacctg cgagtcgtgc agcatgtgga tgccgacctc gtcgtagtcc cacgaatcgt
      181 tcggcgtgta ctggaaatac cacgcgagct tgccggtgtc gatgttgatc gccaccgcgg
      241 aattcgtgta cagattgtcg ccggcgcgcg cctgcggatc gtattgcggt atcgggttgc
      301 ccgtgcccca gatcgtgagg cgggtcacgg gatcgtacga ccccgtctgc cacaagccgc
      361 cgccgccggt tttccacgcc tggtttttgt ccttccatgt ttcgctgccc ggctccccgg
      421 gcgcgggcac cgcataccag cgccacaact cgttgccggt ccgcgcatcg agtgccgcga
      481 tccagccgcg cgtcttgccg tcgccggcgc cgttcgcaat gatgactttg ccatccgcgg
      541 tgatcggtgc agcgaagaac cgctccttgc ggccgaattc gttggcgacg aggatcttct
      601 tgtcccacac gatctcgccg ttgtcgcggt tgatgccgat cacgcgcccg tcgggcaggt
      661 tcgcgatcac gaggtcttcc cacagcgcga tgccgcgcgt gcgcgagaca ttgcccttgc
      721 gcgtgacgcc ggggtcgcag atccagacga attcgccttt gcccgggttg cgcgcatcga
      781 tcttgtagat cgtgccccag ccgtcggtcg tgtacatgta gccgttgtcg atcagtgggt
      841 tgacctggct ctcggggccg ttggcgccga cgtcctgcat gccgccgagc gccatcgccc
      901 acaccatgcg caggtttttc acgttgtcgc gattaatctg cgtgagctta gagtagcgca
      961 gcgagccgta atcgccgttc atcatgagcc agttctgcgg ctcgttctgc gcgttcaaca
     1021 gccggtcgcg gttgacggtc atcgagtagt ccgcggccgc cgcagtgagc gagaggccga
     1081 acgtacaggc gccaacgatt ttgacgatac gctgcatgat gtaattcccc ctgaggtaaa
     1141 gtcccgcgcc ggtcgtggct gaatctatgc cggcttggcg gaactgttgg taatctgcca
     1201 ctagtggcgc gcgctgtcaa agcgcgccgc ggcgggcgaa tggatttacg accccgcgga
     1261 aacacggccg ttcatggttc gatacctgag cacgaacggt tctaatgatt tttgagacgg
     1321 tttctagtcg cttctcgccg ggggcgacac cgacacgccg cgcaccgcgt gatccgcgag
     1381 catgcgcgcg accgctcccg acgctttgac gtcctcgatg aaatcgcgca cgtagcgcgc
     1441 ggccgcaacg cggtctttgg gcgtcgcgat ggtttgctcg atcgccatga agcgcccggc
     1501 gagcacgcgc gagccgggca gcttctcgga ttcgatgacc agccgcggcc ggatgccgac
     1561 gagcgcgtcg agcttgccgg agacgaacac atcgtaggcg ccgaaagtgc cgggacccgc
     1621 gacgagtttc gcgtgcttca gattgcggct cagaaagaat tcgtacgcgc tcttggcggc
     1681 gattgcgacg cgcatgccgt cgcggtcgat gtcggccacg tcgaggatct gcgagcccgc
     1741 cggcacgaga taggtcgcgt cgatgtcgac ataggcggga ctgaatgtga tt
//
LOCUS       DATANS010000135         2244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_49190, whole genome shotgun sequence.
ACCESSION   DATANS010000135 DATANS010000000
VERSION     DATANS010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49190"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VHT22_02015"
     CDS             <1..466
                     /locus_tag="VHT22_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase"
                     /protein_id="HEX3136356.1"
                     /translation="RSALLLGKAKDNNASCAIGPFIRVFDDGFSLDDVRNAEITLDVY
                     GPEGYHLRGASSMTKISRDPEDLVAQAIGANHQYPDGFMLFLGTMFAPVEDRGAPGMG
                     FTHKSGDRVVIAAARLGALENQVTTSDKAPPWTFGTGELMRNLSARGLLRAG"
     gene            482..1921
                     /locus_tag="VHT22_02020"
     CDS             482..1921
                     /locus_tag="VHT22_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX3136357.1"
                     /translation="MNDLHRNYIAGEWAPGAETANNINPSDLSDVIGVYARADRTQAD
                     AAIGAARAAFPAWAASSVQDRFNWLDTIGNTILARKDELGRLLSREEGKTLAEGVGEA
                     ARAGQIFKFFAGETLRLAGEKIPSVRPGIDVEITREPIGVVGIITPWNFPIAIPAWKI
                     APALAYGNCVVFKPAELVPGSAWALAEIIVNAGVPKGVFNLVMGRGSIVGEAMINDRR
                     VDGISFTGSVATGHAIAAKAIKRMAKLQLEMGGKNPLVVLDDADLGVAVNCAINGAFF
                     STGQRCTASSRLIVGAGIHDRFVSALSERLSKLKVDDALAPGTDIGPVVDETQLKQDL
                     EYIGIGQKEGAKLVAGGEVLQRSKKGHYLAPALLTEATNAMRTSREEIFGPVASVIRV
                     RDYEEALSVANDTDFGLSAGIVTTSLKHASHFKRSSEAGMVMVNLPTAGVDYHVPFGG
                     RKGSSYGPREQGRYAREFFTVVKTAYTLP"
BASE COUNT          417 a          723 c          709 g          395 t
ORIGIN      
        1 gcgcagtgca ctgttgctcg gcaaggccaa ggacaacaac gcctcgtgcg cgatcggtcc
       61 attcatccgg gtgttcgacg atggcttctc gctggatgac gtgcgaaacg ccgagatcac
      121 gctcgatgtg tacggccccg aaggctatca cctgcgcggc gcgagctcga tgaccaagat
      181 cagccgcgat cccgaggacc tggtcgcgca ggcgatcggc gcgaaccacc agtatcccga
      241 cggtttcatg ctgttccttg gcacgatgtt cgcgccggtc gaggatcgcg gcgcgccggg
      301 catgggcttc acgcacaagt ccggagatcg cgtcgtcatc gcggcggcgc gcctgggcgc
      361 gctcgagaat caggtgacga ccagcgacaa ggcgccgccc tggacattcg gcaccggcga
      421 attgatgcgc aacctttcgg cgcgcggact gctgcgcgcc ggatgaccac gaggaaccat
      481 gatgaacgac ctgcaccgca actacatcgc cggcgaatgg gcacccggtg ccgaaaccgc
      541 caacaacatc aatccgtccg acttgagcga cgtcatcggc gtctatgcgc gcgccgaccg
      601 gacccaggcc gacgcggcga tcggcgccgc ccgcgcggcg tttcctgcgt gggccgcatc
      661 ctcggtgcag gatcgcttca actggctcga cacgatcggc aacacgatcc tcgcgcgcaa
      721 ggacgagctc gggcgcctgt tgtcacgcga ggaaggcaag acgctggccg agggcgtcgg
      781 tgaagcagcg cgcgccggtc agatcttcaa gttcttcgcc ggcgaaacgc tgcgcctggc
      841 cggcgaaaag attccttcgg tccgtcccgg catcgacgtc gagatcaccc gcgagccgat
      901 cggcgtcgtc ggcattatca cgccgtggaa ctttccgatc gcaattcccg cctggaaaat
      961 cgcgccggct ctcgcttacg gcaattgcgt cgtgttcaag ccggccgagc tcgtgcccgg
     1021 atcggcgtgg gcgctcgccg aaatcatcgt caacgctggc gtgcccaaag gcgtattcaa
     1081 cctcgtcatg ggacgcggca gcatcgtcgg cgaggcgatg atcaacgacc gccgcgtcga
     1141 cggcatcagc ttcaccggct ccgtcgcgac cggccacgcg atcgcggcaa aagcgataaa
     1201 gcgcatggcc aagctgcagc tcgaaatggg cggcaagaat ccgctggtcg tcctggacga
     1261 cgccgacctg ggtgtggcgg tcaattgcgc gatcaatggg gctttctttt cgaccgggca
     1321 gcgctgcacg gcctcgtcgc gtctgatcgt cggcgcgggc atccacgacc gtttcgtttc
     1381 cgcgctgtcc gaacggctga gcaagctcaa ggtcgacgat gcgcttgcgc cgggaaccga
     1441 tatcgggccg gttgtcgacg agacacaact caagcaggac ctcgaataca tcggcatcgg
     1501 ccagaaggaa ggagcgaagc tagtggccgg cggcgaagtt ctccagcgca gcaagaaagg
     1561 gcattacctg gcgccggcgc tgctgaccga ggcgaccaac gcgatgcgca cgtctcgaga
     1621 agaaatcttc ggaccggtcg cgagcgtgat ccgcgtcagg gactacgaag aagctctttc
     1681 tgtagccaac gacaccgact tcggtctttc cgccggcatc gtgaccacat cgctgaagca
     1741 cgcctcgcat ttcaagcgca gcagcgaggc cggcatggtg atggtcaacc tgccgaccgc
     1801 cggggtcgac taccacgtcc cattcggcgg ccgcaaaggc tcgagctacg gtccgcgcga
     1861 gcagggacgc tacgcccggg aattctttac cgtggtcaag accgcctata cgctgccgtg
     1921 atcgaagccc ggtcgcgtcc aacgccggac ttagtgtcga caattcttac gaactgctgg
     1981 accttattct cggccttgcg cgctaagcga ttgtaatcat tgcacttgcg ctcatcggta
     2041 cggttgttgc gtacaacgtg tcgccaagta actcgtcagc gttagcgggg gtccgatgcc
     2101 tttccagccc aagtccagaa ttttgagcgt gttgcggtgg gccacagccg ttgccgcact
     2161 ctttctatgg gcggcgtcag cggccggcgc tccgttcttt ttcagcactg gagatccgga
     2221 tgggctgatc gcgacagcgt ctcg
//
LOCUS       DATANS010000136         1754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_720867, whole genome shotgun sequence.
ACCESSION   DATANS010000136 DATANS010000000
VERSION     DATANS010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720867"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..>1754
                     /gene="hscA"
                     /locus_tag="VHT22_02025"
     CDS             152..>1754
                     /gene="hscA"
                     /locus_tag="VHT22_02025"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEX3136358.1"
                     /translation="MALLQIAEPGEAPAPRRPRLAVGIDLGTTNSLVATVRDGSADVL
                     PDAQQRRLLPSIVRYAPEGVEVGYDAQAAQIADPQNTIVSVKRMMGRALPDLADARRF
                     PYRFVDSPGIVQIATRAGIRTPVEISAEILGALRERAEKTLGGALDGAVVTVPAYFDD
                     AQRQATKDAARIAGLNVLRLLNEPTAAAIAYGLDNASEGTYAVYDLGGGTFDLSILKL
                     SRGVFEVLATAGDSALGGDDFDHRIYCWALEAARVGPVSPGDTRMLMTRAREVKEALT
                     TSDEVAFNATLQSGDTIAATLAAAEFESISQTLVAKTLAPVRKALRDARLTPADIKGV
                     VMVGGATRMPQIQRAVAEFFGQAPLNNLNPDEVVALGAAIQANVLAGNRATDEDWLLL
                     DVIPLSLGLETMGGLTEKIIPRNATIPIARAQEFTTFKDGQTAMALHVVQGERERVAD
                     CRSLARFELRGIPPMVAGAARIKVTFQVDADGLLAVSASEQTTGVEASIVVKPSYGLS
                     DDEIARMLQESFAYAAPDMVARAAAE"
BASE COUNT          296 a          581 c          605 g          272 t
ORIGIN      
        1 gcgccgagat tgcggcgctc gagccggcgc tggccgagac gctggatagc cggcagagct
       61 gggaatcggc gcgcggcagc gtgcgtgagc tccagttcct gtccaagctc gccgccgaca
      121 tcgacgccgc catcggcggg atcgaaaact gatggctctg ctgcagattg ccgaaccggg
      181 ggaggcgccc gcgccgcgac ggccgcggct cgctgtcggc atcgacctcg gaaccaccaa
      241 ttcgctggtc gccaccgttc gcgacggcag cgccgatgtg ctgcccgacg cgcagcagcg
      301 ccgcctgctg ccgtcgatcg tgcgctacgc gcccgaaggc gtcgaagtcg gctacgacgc
      361 gcaggcggcg cagattgccg atccgcagaa caccatcgtc tcggtcaagc gcatgatggg
      421 acgggcgttg cccgatctcg ccgatgcgcg ccgtttcccc tatcgctttg tcgactcgcc
      481 cggcatcgtg cagatcgcga cgcgcgccgg catccgcacg ccggtcgaaa tctcggccga
      541 gattctgggc gcgctgcgcg aacgcgcgga aaaaacattg ggtggcgcgt tggacggcgc
      601 agtcgtcacc gtacccgcct atttcgacga tgcgcagcga caggcgacca aggatgcggc
      661 gcggatcgcc ggtctcaatg ttctgcgtct gctgaacgag ccgacagcgg ccgcgatcgc
      721 ctacggcctc gacaacgcct cggaaggaac ctacgccgtc tatgacctgg gcggcggcac
      781 gttcgacctg tcgatattga agctctcgcg cggcgttttc gaagtgctgg cgaccgccgg
      841 cgactcggcg ctgggcggcg acgacttcga ccaccgcatt tattgctggg cactagaggc
      901 cgcacgcgtc gggccggttt cgccgggcga tacccggatg ctgatgaccc gggcgcgcga
      961 ggtcaaggaa gcgctgacga cgagcgacga ggtcgcgttc aacgccacgc tgcagtcggg
     1021 cgacacgatc gctgcgacgc tcgccgcagc agaattcgag tcaatcagcc agacgctggt
     1081 cgcgaagaca ctcgctccgg tcaggaaggc gctgcgcgat gcccggctca caccggcgga
     1141 catcaaaggc gtggtgatgg tcggcggcgc gacccgcatg ccgcagatcc agcgggcagt
     1201 cgccgaattt ttcggccagg cgccgctgaa caatctgaat ccggacgagg tcgtggcact
     1261 cggcgctgcg atccaggcca atgtgctggc cggcaaccgt gcgaccgacg aggattggct
     1321 gctgctcgac gtgattccgc tgtcgctggg actcgagacg atgggcggcc tcaccgaaaa
     1381 aatcatcccg cgcaacgcga cgataccgat cgcgcgcgcg caggaattca ccacgttcaa
     1441 ggacgggcag accgcgatgg cgctgcacgt cgtgcagggc gagcgcgagc gggtcgccga
     1501 ctgccgttcg ctcgcgcgct tcgaattgcg cgggattccg ccgatggtcg ccggcgccgc
     1561 gcggatcaag gtgacgttcc aggtcgacgc cgatggcctg ttggcggtat ccgccagcga
     1621 gcagacgaca ggcgtcgagg catcgatcgt ggtcaagcct tcgtacggtc tgtcggacga
     1681 cgagatcgcc cgcatgctgc aggaatcatt cgcctatgcc gcgccggaca tggttgcgcg
     1741 cgctgcggcc gagg
//
LOCUS       DATANS010000137         4957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_553578, whole genome shotgun sequence.
ACCESSION   DATANS010000137 DATANS010000000
VERSION     DATANS010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553578"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VHT22_02030"
     CDS             complement(<1..306)
                     /locus_tag="VHT22_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136359.1"
                     /translation="MPHFSIRTLSIHMRHRAAVAAFALLPLAFACESMAQTPNGAAPD
                     PPPQLKSSPVLEPPPPKALRLTPGANPMAPEREPIFLRADKLEGDNQQWIEASGHAEL
                     "
     gene            339..1373
                     /locus_tag="VHT22_02035"
     CDS             339..1373
                     /locus_tag="VHT22_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX3136360.1"
                     /translation="MSSDVRLHALNAWLERVLPAPISSIAPATSDASFRRYFRITLAG
                     EVSLPADAPPVRTLIAMDAPPAQEDSAAYVAVASLLAAAGVHAPTVHAQDLSAGFLLL
                     SDLGTRTYRDALDDATAPALYRDACAALIAWQLASRDSVLPRYDEAMLARELAIFPDW
                     YVARHLGITLTVPQRATLDSAFRQVLDSALRQPLVYVHRDFHSRNLMVTKPNPGVLDF
                     QGAVYGPISYDLVSLLRDAYIDWSEEQELDWAVRYWERAREARLPVAEDFGDFWRDFE
                     WMGVQRHLKILGIFARLAHRDGKHGYLADLPRVMRYLRRACDRYRALGPLLQLLDELE
                     ARQPVTGYTF"
     gene            1373..2122
                     /locus_tag="VHT22_02040"
     CDS             1373..2122
                     /locus_tag="VHT22_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX3136361.1"
                     /translation="MDAAIDPHHGTSPPTAMILAAGRGERMRPLSDLTPKPLLSVGGK
                     PLIVWQIERLVAAGFANLVINVAHLGAAIEKTCGDGRRFGATIRYSREGEPLEVGGGI
                     ATALDLLDRDATLVVSGDIYTGYDYASLLPRLAAMKATREAPYAHMVMVPNPDYHRAG
                     DFALVDGRLSLAARPQLTFGNIALYRSALFEALPRGEKIRMLPLYRDWIARGWVSGER
                     YDGVWANVGTPDELRELDGQLTAPPSEKVLR"
     gene            2119..4173
                     /locus_tag="VHT22_02045"
     CDS             2119..4173
                     /locus_tag="VHT22_02045"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX3136362.1"
                     /translation="MNTVAQITEINPLLDFSGLPRFADIRPEHVAPALAALLGEARAT
                     IERVATAPGSPTWDSFVQPLSDALDRLDRAWTQVGHLNAVVNTPELRAAYNENLPKVT
                     SFYTELGQDGRLFGRYRALAASPEFAAADPARRRVVDNALRDFRLSGAELAPAPKARF
                     KAVEEELASLSSRFADNVLDATNAFSLYVEDASRLAGMPADVVAAARASAQADGKPGW
                     KLTLHMPSYLPVLQYADDRGLRETLYRANATRASEFGKPEWDNGPLVDKILALRAEAA
                     ALLGYASYAEVSLVPKMAGSPAEVIDFLHDLARRAKPYAERDMAELSEFARERLGLSE
                     LAAWDLPYVSEKLRQARYSYSDQEVKQFFSEDRVLSGMFRVVETLYGITVRASTAPAW
                     DPAVRFFELTDRTGAPVGKFYLDLYARPGKRDGAWMDAAIKYRHTNGRAQHPVAFLVC
                     NFAAPVAGRPALFTHREVTTLFHEFGHGLHLLLTRVDVPGASGLEGVEWDAVELPSQF
                     MENFCWEWDVVEKMSAHVETGQPLPKAMFDRLLAARNFQSGMQTVRQLEFALFDMHVH
                     HDIDRSVSPLALAWSVRREVAVVPRPDYDRSLPHGFSHVFAGGYAAGYYSYKWAEVLS
                     ADAYSLFEEQGVLSATAGARFRDEILARGGSRPALDSFVAFRGRKPEISALLRHNGML
                     AS"
     gene            4197..4796
                     /locus_tag="VHT22_02050"
     CDS             4197..4796
                     /locus_tag="VHT22_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX3136363.1"
                     /translation="MSRTSTPARCAAGLAGLLLLASACAIAQTQQVYRYLDSDGRVVY
                     SDKPPPADARNAQAKRIGRNSIETSELSFATQQAQERFPVTLYTFSCGVVCDTAQGLL
                     NKRGVPHTVVDVAQGDGADRLKRLTNGLEAPALQVGEQVATGFNETKWQTMLSDAGYP
                     KTPPPRTTAVGRPPTPAPLDTKTTQVVTPEVAPKGSYPP"
     gene            4799..>4957
                     /locus_tag="VHT22_02055"
     CDS             4799..>4957
                     /locus_tag="VHT22_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX3136364.1"
                     /translation="MRVATWNVNSLNVRLPRLLDWLAANAPDIVGLQETKLEDGKFPV
                     MELAAAGYA"
BASE COUNT          810 a         1651 c         1615 g          881 t
ORIGIN      
        1 aagttccgcg tgccccgacg cttcgatcca ctgctgattg tcgccttcga gcttgtcagc
       61 acgcagaaag atcggctcgc gctcgggcgc catcggattc gcgcccgggg tcagccgcag
      121 cgccttcgga ggaggcggct ccagcaccgg cgacgatttg agttgcggtg gcggatcagg
      181 cgcagcgcca ttgggagtct gcgccatcga ttcgcacgcg aacgcgagcg gcaacagggc
      241 aaacgcggca acggcggcgc gatggcgcat gtgaatcgac aatgttcgga tgctaaaatg
      301 gggcattcta cccgcctctt ttgtcagatc cctcccgcat gagttccgat gtccggttgc
      361 acgcgctcaa cgcgtggctc gagcgggtgt tgcctgcccc gatcagctcg atcgcgccgg
      421 caacgtccga tgcgagcttt cgccgctact tccggatcac gctggccggc gaggtctcgc
      481 ttccggccga tgcgccgccg gtgcgaacac ttatcgcgat ggatgcgccg ccggcgcagg
      541 aggatagcgc agcatacgtg gcggtcgcct cattgcttgc cgcagccggt gtccacgcgc
      601 cgacagtgca tgcgcaggac ttgtcggccg gtttcctgct gctgtccgat ctgggcacgc
      661 gtacctatcg cgacgcgctc gacgacgcga ccgcgccggc gctgtaccgc gatgcctgcg
      721 ctgcgctgat cgcctggcag ctcgccagtc gcgactcggt actgccccgc tacgacgagg
      781 cgatgctggc gcgcgagctc gcgatatttc ccgactggta tgtggccagg catctcggta
      841 tcaccctgac cgtgccgcag cgcgcgacgc tcgacagcgc cttccgtcag gttctcgaca
      901 gcgctctcag gcaaccgctt gtctatgtgc accgcgattt tcattcccgc aatctgatgg
      961 tgacaaaacc aaatccaggc gtcctcgatt ttcagggggc ggtgtacggt ccgatcagct
     1021 atgacctggt ctcgctgctg cgcgatgcct acatcgactg gagcgaggag caggagctcg
     1081 actgggccgt gcgctattgg gagcgcgcgc gcgaagcccg gttgccggtt gccgaggatt
     1141 tcggcgactt ctggcgcgac ttcgagtgga tgggtgtgca gcgtcatttg aagatcctgg
     1201 gcattttcgc ccggcttgcg catcgcgacg gcaagcatgg ctacctcgcc gacctgcctc
     1261 gcgtcatgcg ctatctgcgc cgtgcctgcg atcgctaccg ggcgcttggc ccgctgctgc
     1321 aattgctcga cgagctcgaa gcaaggcagc cggtgaccgg gtacacattc tgatggacgc
     1381 cgcgatcgat ccccatcacg gcacttcgcc gccgacggcc atgatcctgg ccgccggccg
     1441 cggtgagcgc atgcgcccgc tgtccgatct gacgcccaag ccgcttctct ccgtcggcgg
     1501 caagccgctg atcgtatggc agatcgagcg cctggtcgcc gcaggcttcg ccaatctggt
     1561 catcaacgtc gcccacctcg gcgcggcgat cgagaagacc tgcggcgacg gccgccgatt
     1621 cggcgccacg atccgctact cgcgcgaggg cgagccgctc gaagtcggcg gcggcatcgc
     1681 caccgcgctc gatctcctcg atcgggacgc aaccctggtg gtcagcggtg acatctatac
     1741 cggctacgac tatgcgtcgc tgctgccgcg tctggccgcg atgaaggcaa cacgtgaggc
     1801 tccgtacgcg cacatggtga tggtgccgaa tcccgattac catcgcgccg gcgatttcgc
     1861 gctggtcgac ggcaggcttt cgctcgccgc gcgcccgcaa ctgacgttcg gcaacatcgc
     1921 gctctaccga agtgcgctgt tcgaagcgct gccgcgcggc gagaagatca ggatgctgcc
     1981 gctgtatcgc gactggatcg cccgcggctg ggtcagcggc gaacgctacg acggcgtctg
     2041 ggcaaatgtc ggcacgcccg acgaattgag agagctagac ggtcaactta cggcaccgcc
     2101 aagcgagaaa gtcctgcgat gaataccgtt gcacagatca ccgagatcaa tccgctgctt
     2161 gatttttccg gcttgccgcg cttcgccgac atccgacccg agcatgttgc gcccgcattg
     2221 gctgcgttgc tcggcgaggc ccgcgccacg atagagcgcg tcgcgaccgc gcccggatcg
     2281 ccgacctggg attcttttgt gcagccgttg tccgatgcgc tcgaccgtct cgaccgcgcc
     2341 tggacgcagg tcggacatct gaacgccgtc gtcaacacgc ccgagctgcg cgcggcatac
     2401 aacgaaaact tgccgaaggt gacgtccttc tataccgagc tcgggcagga tgggcgcctg
     2461 ttcggccgtt accgcgccct tgccgcctcg cccgaattcg ccgccgccga tccggcccgg
     2521 cgccgcgttg tcgacaatgc gctgcgcgat tttcggctgt cgggcgcaga gctggcgccc
     2581 gcaccgaagg cccgcttcaa ggcggtcgag gaagagctcg ccagtctctc ctcgcgtttc
     2641 gccgacaatg tgctcgatgc caccaacgcc ttttcgcttt acgtcgagga cgcatcgcgc
     2701 ctggccggca tgccggccga cgtcgtcgct gcggcgcgcg cttcggcgca agccgatggc
     2761 aagccgggtt ggaagctcac cttgcatatg ccgtcttacc tcccggtgct gcagtacgcc
     2821 gacgatcggg gcctgcgcga aacgctttac cgcgccaatg cgacgagggc cagcgaattc
     2881 ggcaaaccgg aatgggacaa cgggccgctg gtcgacaaga tcctcgcgct gcgcgccgaa
     2941 gccgccgcgc tgctcggcta cgccagctac gctgaagtgt cgctggttcc gaagatggcg
     3001 ggctcgccgg ccgaggtgat cgatttcctg cacgatctgg cgaggcgggc gaagccctat
     3061 gccgagcggg acatggccga gctttcggaa ttcgcccgcg aaaggctcgg actgtcggag
     3121 ctcgccgcct gggacctgcc ttatgtctcg gaaaagctgc gccaggcgcg ctacagctat
     3181 tccgatcagg aggtgaagca atttttttcc gaagaccgcg tgctgtccgg catgtttcgc
     3241 gtggtcgaaa ccctgtatgg gatcacggtg cgcgcttcga cggcgccggc gtgggatccc
     3301 gccgtgcgct ttttcgagct caccgatcga accggcgcgc cggtcggaaa gttctatctc
     3361 gatctgtacg cgcggccggg aaagcgcgac ggcgcgtgga tggacgccgc gatcaagtat
     3421 cgccacacga atggccgagc ccagcatccg gtcgccttcc tggtatgcaa tttcgcggcc
     3481 cccgtcgccg gcaggcctgc actgttcacg caccgcgagg tgacgacgct gttccatgaa
     3541 ttcggccatg ggctgcatct gctgctcacg cgcgtcgatg tgccgggcgc gtccggcctc
     3601 gagggtgtcg agtgggatgc ggtcgagctg cccagccagt tcatggaaaa cttctgctgg
     3661 gagtgggacg tcgttgagaa aatgagcgcg cacgtcgaga cgggtcagcc gttgcccaag
     3721 gccatgttcg accgcctgct cgcggccagg aatttccaga gcggcatgca gacggtgcgg
     3781 cagctcgaat tcgccctgtt cgacatgcac gtgcaccacg acatcgaccg gtcggtctcg
     3841 ccgctggcgc tcgcctggtc ggtgcgccgc gaagtggccg tcgtgccccg ccccgattac
     3901 gaccgcagcc tgccccatgg tttcagtcat gtcttcgctg gcgggtatgc ggcgggctat
     3961 tacagctaca aatgggccga agtgctgtcg gctgacgcct acagcttgtt cgaggagcaa
     4021 ggcgtgcttt cggcgaccgc cggcgcgcgg ttccgagacg agatcctcgc ccggggcggc
     4081 agccggccgg cgttggactc attcgtggcc tttcggggcc gaaagccgga aatcagtgca
     4141 ttattgcggc ataatggtat gctagcgtca tagcatgcga gcaagggagg cttaagatga
     4201 gcaggacatc cacgccggca cgctgcgccg cgggcctcgc cggcctgctg ctgctggcga
     4261 gcgcatgcgc gatcgcgcaa acccagcagg tctaccgcta cctcgacagc gatggccgcg
     4321 tcgtctattc ggacaagccg ccgccggccg acgcccgaaa cgcgcaggcc aagcgcatag
     4381 gccgcaacag catcgaaacc agcgaattgt cgtttgcgac gcagcaagcg caggagcgct
     4441 tcccggtcac tctgtacaca ttttcctgcg gcgtcgtctg cgacaccgcg cagggccttc
     4501 tgaacaagcg cggcgtgccg cacacggtcg tcgacgtcgc gcagggggac ggcgccgacc
     4561 gcctgaagcg cctgaccaac gggctcgagg cgccggcgtt gcaggtcggc gagcaggtgg
     4621 cgaccggctt caacgagacc aagtggcaga ccatgctttc cgatgccggc tatccgaaga
     4681 cgccgccgcc gcgaacgacg gcggtcgggc ggccgccgac gccggccccg cttgacacca
     4741 agaccacgca ggtcgtcacg cccgaggtgg cgccgaaagg ctcgtacccg ccgtgacctt
     4801 gcgggtcgcg acctggaacg tcaactcgct taatgtccgg ctgccgcggc tgctggactg
     4861 gcttgccgcg aacgcaccgg acatcgtcgg gctgcaggaa accaagcttg aagacggcaa
     4921 gtttccagtc atggagcttg ccgccgcagg ctacgcg
//
LOCUS       DATANS010000138         2912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_342108, whole genome shotgun sequence.
ACCESSION   DATANS010000138 DATANS010000000
VERSION     DATANS010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342108"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VHT22_02060"
     CDS             complement(<1..842)
                     /locus_tag="VHT22_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3136365.1"
                     /translation="MTTVSAPAIGRDPAVATVVIALWALLLLFVVYPLVTLLGRVLFD
                     HGAFTTAGIAAILTDRHQVRAFWNSLLLALLVGSGGTIIGFVFAFTAARSRLPRWLLT
                     VIDAAVLLPLVSPPFTTAIAMIFSFGPRGLITYELLGLKGVTVYGLMSTLSSEILTYF
                     PIAYLTLRPLLAAIDSNLEGMALSLGASRWRVFRTVTLPLCIPGLANAFLLLFAASLA
                     DFATPLILAGNNFPVLPTQAYLQITGLFDLRGGAALSFMLLVPALGVFLLQRYWVSRR
                     FYVTV"
     gene            complement(906..1934)
                     /locus_tag="VHT22_02065"
     CDS             complement(906..1934)
                     /locus_tag="VHT22_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3136366.1"
                     /translation="MRIVTRWVRTIVALPLLALALGASWPAIAADTVNVYSIWPENWA
                     RPMFEEFEKATGVKVNFVRFSSGEALARVIAEKTNPRVDVLFGGPVETFAAGINEGLF
                     EPYKSPSFARLPERFREPSGQWTAIADDPLVFMTNDKFLKENNLKAPTSWNDLLAAPY
                     KNMLQMADARTSGTAVTRIFSILEVNGRDEDKAFAYMKKLRPNVQLYTKSGGGGTLPV
                     GLGQAGGGIFFIVDALDTKAKGYDVTISFPKEGIGTAAEGIALIKGAKNPELGKKLID
                     WATSPAMQNQFAKYKINFVPAHPDVAMEPSLAAVIKGANIFPIDAAYAGANRKHVVER
                     WVAEVLNP"
     gene            complement(2047..2859)
                     /locus_tag="VHT22_02070"
     CDS             complement(2047..2859)
                     /locus_tag="VHT22_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136367.1"
                     /translation="MRFSCRRACAAAALVVVALIPAAEVHAAKATECTKIGICYCVND
                     GLKATIAAKVDAFRQAIAEQRKAGKAIGYLSVPLTSAGGGNFDINKEVAERARGAIEK
                     RFGADYVFVLNPGTKEADLPRSSTGADYMLMWTSLLEGADGLGEFDFAYFTGPQDFAR
                     YFGFDGSNDMGKLDAYFDKRVAADPAFAKAVQGGLSKAAFRKYYALRASTTVSRGAHD
                     EWNIFRMLNEKRRANDQYGPGGQIAVLFDGAAIAPPQGEAAVSDGYVGKCQP"
BASE COUNT          529 a          922 c          954 g          507 t
ORIGIN      
        1 accgtcacgt aaaagcggcg gctgacccag tagcgctgca acaggaacac gccaagcgca
       61 ggcacgagca gcatgaacga gagcgccgcg ccgccgcgca ggtcgaacag accggtgatc
      121 tgcaggtatg cctgcgtcgg aagcaccgga aaattattgc cggcgaggat taacggggtg
      181 gcgaagtcag ccagcgatgc ggcgaagagc agcaggaacg cattggcgag tccggggatg
      241 cacaggggca gcgtcaccgt gcggaacacg cgccagcgcg atgcgcccag cgacagcgcc
      301 attccttcga gattggagtc gatggcggcc agcagcggac gcaaggtcag ataggcgatc
      361 gggaaataag tcaggatttc cgacgacagc gtgctcatca ggccgtacac ggtgacgccc
      421 ttcaggccaa gcagctcgta ggtgatcagg ccgcgcgggc cgaacgaaaa gatcatcgcg
      481 atcgcggtcg tgaacggcgg tgagaccagc ggcagcagca ccgcggcgtc gatcaccgtc
      541 agcagccatc gcggaagtct cgagcgggcc gcggtgaatg cgaacacaaa cccgatgatc
      601 gtgccgccgc ttccgaccag cagcgcgagc agcaggctgt tccagaacgc gcggacctgg
      661 tgccggtcgg taaggatcgc ggcgatgccg gccgtcgtga atgcgccgtg gtcgaacagg
      721 acgcggccca gcagtgtgac cagcgggtag acgacgaaca gcagcagcag cgcccacagc
      781 gcgataacca ccgtggcgac tgccgggtcg cgaccgatgg cgggcgccga aacggttgtc
      841 acagcggtga cggcgcacgg cgatcccgct ccccgtccgc gggagccgga ttgcgcaggc
      901 gctggtcagg gattgagaac ttcggcgacc cagcgctcga cgacatgctt gcgattcgcg
      961 cccgcgtacg ccgcgtcgat cggaaagatg ttggcgccct tgatcacggc ggcgaggctc
     1021 ggctccatcg cgacgtccgg atgcgccgga acgaaattga tcttgtattt cgcaaactgg
     1081 ttctgcatcg ccggcgacgt cgcccagtcg atcagcttct tgccgagctc gggatttttc
     1141 gcgcccttga tcagtgcgat gccctcggct gccgtgccta tgccttcctt cgggaagctg
     1201 atcgtcacgt cgtagccttt cgccttcgtg tccagcgcgt cgacgatgaa aaagatgccg
     1261 ccgcctgcct ggccaagacc gaccggcagc gtgccgccgc cgccgctctt ggtatagagc
     1321 tggacgttgg gccgcaattt tttcatgtag gcgaacgcct tgtcctcgtc gcgcccgttg
     1381 acttcgagga tcgaaaagat ccgggtcaca gcggttcccg acgttcgcgc gtcggccatc
     1441 tgcagcatgt tcttgtacgg cgccgccagc aggtcgttcc acgacgtcgg cgccttcagg
     1501 ttgttctctt tcagaaactt gtcgttggtc atgaacacca gcggatcgtc ggcaatcgcc
     1561 gtccattgcc cgctgggctc gcggaaccgc tcgggcaacc tcgcgaacga cggcgacttg
     1621 tagggctcga acaggccttc gttgattccg gcggcgaacg tctcgaccgg gccgccgaac
     1681 agcacatcga cgcgcggatt ggtcttttcg gcgatcacgc gtgcaagcgc ctcgccggac
     1741 gagaagcgga cgaagttgac tttaacgcct gttgcctttt cgaactcctc gaacatcggc
     1801 cgcgcccagt tctccggcca gatcgaatag acgttgacgg tgtccgccgc tatggcaggc
     1861 cacgaggcgc ctagcgccag cgcgagcagg ggcagggcga cgatggtgcg gacccagcgg
     1921 gtgacgatcc tcatgaacgt tcctccttgt ggcgtttttg ccgccatccc gcaacgtcct
     1981 ggacggcttg cgacctggcg acggtacttt actggcgcgg caccggtcaa acgcgcccgg
     2041 cgcccgctaa ggctggcact tgccgacata gccgtcggac acggcggctt cgccctgcgg
     2101 cggcgcaatc gccgcgccgt cgaaaagcac agcgatctga cctccggggc catattggtc
     2161 gttggcccgc cgcttttcgt tgagcatgcg aaagatgttc cattcgtcgt gcgcgccgcg
     2221 gctgacggta gtcgatgcgc gcagcgcgta gtatttgcgg aaggccgctt tgctgaggcc
     2281 gccttgaacg gccttggcga aggccgggtc cgcggcgacg cgcttgtcga aatacgcatc
     2341 caacttgccc atgtcgttgc tgccgtcgaa gccgaagtag cgtgcgaagt cctgcggccc
     2401 cgtgaaatag gcgaagtcga actcgccgag gccatcggcg ccttccagca acgacgtcca
     2461 catcagcatg taatcggcac cggtgctgct gcgcggcaag tcggcttcct tggtgccagg
     2521 attgagcacg aagacgtagt cggcgccgaa acgcttttcg atcgcccccc tggcccgttc
     2581 cgcgacttcc ttgttgatgt cgaagttgcc gccgccggcg gaggtgagag gcacgctgag
     2641 gtagccgatg gccttgccgg ccttgcgttg ctcggcgatc gcttgacgga aggcgtcgac
     2701 cttggctgcg atcgtcgcct tcagcccgtc gttgacgcag tagcagattc caatcttcgt
     2761 gcattcggtg gctttggcgg cgtggacttc ggccgccggg atcagcgcga cgacgaccag
     2821 tgccgcagca gcgcaggcac gacggcatga gaaacgcatg gcatgatctc cgtgcagacg
     2881 gacagccgct aacttaaacc cgggccgccg cc
//
LOCUS       DATANS010000139         1752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_49314, whole genome shotgun sequence.
ACCESSION   DATANS010000139 DATANS010000000
VERSION     DATANS010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49314"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VHT22_02075"
     CDS             <1..885
                     /locus_tag="VHT22_02075"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HEX3136368.1"
                     /translation="RPIEVVAWTNEEGGRFAPCTMGSMVFTGARPLADVLEVTDNEGV
                     KLKEALAQTLAATPEAKPREFKSPAAGYIEAHIEQGPLLENARKTIGIVTGIQGLRWF
                     NVEVSGKTDHAGTTPLALRKDAVRDSIAIINALTELTHDPQDVTRFTVGRMLVTPNSP
                     NSVASHVLFSVDIRHPDPQVVARLGAAVEPLARKAAKFCEVKVTPTLHDDPCVFDAEV
                     AGAIETAAQTLDLPHMRLSSGASHDAMYMARVCPTAMIFVPCEKGISHNEAENATPGD
                     LAAGARVLTAALITLANR"
     gene            916..>1752
                     /locus_tag="VHT22_02080"
     CDS             916..>1752
                     /locus_tag="VHT22_02080"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3136369.1"
                     /translation="MTFDLLIRNGTVVDGTGAAKFSGDVGITAGRITDIGTIPATATA
                     KQVIDAQDKIVCPGFIDPHTHYDPQITWDSVLSSSSEHGITTIVMGNCGVGVAPCRPA
                     DRDLMIQDLVNVESMSQEVLRAGIKWDWETFPQYMDSAAKNASAVNRAFMVPLAPLRT
                     YVMGEAASDRAADKNETEKMAGMLREAIDAGAWGYSTTAILSHVGHHGKPLSARLASR
                     DELAAYSQALRKAGRGIIELALTKRFATLADDEYALLEHLLDHSGRPVTWLSLHNLLE
                     KPD"
BASE COUNT          353 a          564 c          559 g          276 t
ORIGIN      
        1 cgcccgatcg aggtcgtcgc atggaccaac gaggaaggcg ggcgctttgc gccgtgcacg
       61 atgggctcga tggtgttcac gggggcgcgg ccgctcgccg acgttctcga agtgaccgac
      121 aacgaaggcg taaaactgaa ggaagcgctt gcgcaaacac tcgcggcgac gcccgaagca
      181 aagccgcgcg aattcaagtc gccggccgcc ggttacattg aagcgcatat cgagcagggt
      241 ccgctgctcg aaaacgcgcg caagacgatc ggcatcgtca ccggcattca gggcctgcgc
      301 tggttcaacg tcgaagtctc cggcaagacc gaccacgcgg gcacgacgcc gctcgcattg
      361 cgcaaggacg ccgtgcgcga cagcatcgcg atcatcaacg cgctcaccga actcacgcat
      421 gacccgcagg acgtcacgcg cttcacggtc ggccgcatgc tggtaacgcc gaattcgccg
      481 aactcggtcg cgagccacgt gctgttctcg gtggacatcc gccatcccga tccacaagtc
      541 gtggcgcggc ttggcgctgc ggtcgaaccg ctcgcgcgca aggctgcgaa attctgcgag
      601 gtcaaggtga cgccgacgct gcacgacgac ccgtgcgtgt tcgatgcgga ggtcgctggc
      661 gcaatcgaaa ccgccgcgca aacactcgat ctgccgcata tgcggctgtc gtcgggcgcg
      721 agccacgatg cgatgtacat ggcgcgcgtg tgcccgaccg cgatgatatt cgtgccgtgc
      781 gagaaaggca tctcgcacaa tgaagcggaa aacgcgacgc ccggggatct cgccgccggc
      841 gcgcgcgtgc tgaccgcggc gctgataaca ttggcgaatc gttaaacgcc tgaattacaa
      901 ccaactggga ctctaatgac tttcgatctg ctgatcagga acggcaccgt agtcgacggc
      961 acgggcgccg cgaaattttc cggcgacgtg gggatcaccg ccggccgcat caccgacatc
     1021 gggacaattc ccgctacggc aaccgccaag caagtcatcg acgcgcaaga caagatcgtc
     1081 tgccccggct ttatcgatcc gcacacgcat tacgatccgc agatcacgtg ggacagcgtg
     1141 ctctccagct cgtcggagca cgggatcacg acgatcgtga tgggcaattg cggcgtcggc
     1201 gttgcgccgt gccggccggc cgaccgcgac ctgatgatcc aggacttggt caacgtcgaa
     1261 agtatgtcgc aggaagtgct gcgcgccggg atcaagtggg actgggaaac atttccgcaa
     1321 tatatggatt cggcggcgaa gaacgccagc gcggtcaatc gtgcattcat ggtgccgctc
     1381 gcgccgctgc gcacgtacgt gatgggcgag gctgcgtccg accgcgccgc agataaaaac
     1441 gaaaccgaaa agatggccgg catgctccgc gaggcgatcg atgccggtgc gtggggctac
     1501 tccacgaccg cgatcctgtc gcacgtcggg catcacggca agccgctgtc ggcgcgtctc
     1561 gcgagccgcg acgaacttgc ggcctattcg caggctctca ggaaggccgg ccgcggcatc
     1621 atcgagctcg cgctgaccaa gcgcttcgcg accctcgccg acgacgagta cgcgctgctc
     1681 gagcacctgc ttgatcacag cgggaggccg gtgacgtggt tatcgctgca caacctgctc
     1741 gaaaagcccg ac
//
LOCUS       DATANS010000140         2774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_301016, whole genome shotgun sequence.
ACCESSION   DATANS010000140 DATANS010000000
VERSION     DATANS010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301016"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(315..1043)
                     /gene="istB"
                     /locus_tag="VHT22_02085"
     CDS             complement(315..1043)
                     /gene="istB"
                     /locus_tag="VHT22_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX3136370.1"
                     /translation="MERHEIMELLGQLKLAGMRAAYDEVVAAGVRAQHPFQRIFGELL
                     LAQLADHRARSTAYRMGVARFPVMKNLTEFDFTVSPINQGLVRELHEGGFLATQRNAV
                     LIGGTGTGKSHIAIAIGANCVRNGARVRFFTAVDLVNQLEAEGRAGKSGKLADQLVRV
                     DLVILDELGYLPFPVSGGQMLFHLISRLYERTSIVLSTNLAFAEWPSVFADAKMTTAL
                     LDRLTHHCDILETGNESWRFKNRT"
     gene            complement(1030..2556)
                     /gene="istA"
                     /locus_tag="VHT22_02090"
     CDS             complement(1030..2556)
                     /gene="istA"
                     /locus_tag="VHT22_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043766075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX3136371.1"
                     /translation="MVTVETIGRIRRAFWVDKKPIRQIVRELRISRQTVRKAIRGSSV
                     EFRYERQVQPQPRLGAYVARLDGLLEANSKRPARERLTARRLFELLRAEGYEGAYDSV
                     QRHVREWRRQQSRQGPVFIPLWFAPGEAYQFDWSHEVVVLGGVTTTVKVAHIRLCHSR
                     MFLVRAYPRETQEMVFDAHERAFRLFGGACRRGIYDNMTTAVEAVFVGKERRFNRRFL
                     AMCSHYLVEPVACTPAAGWEKGQVENQVGNAREHLFTPRLHFADYAELNAWLEARCLA
                     QARASAHPEQTDKTVWEVFEAERASLIAYRGPFDGFREIEVAVSKSSLVRYDHNRYSV
                     AVKAARRTAQMRVYAERIVVWCDGEIVAEHSRRFGRGQTAYDPWHYLPVLARKPGALR
                     NGDPFRNWDLPPALAQTRRRLAGHEDGDRQFVDILTAVAEAGIEAVEAACAEALSAKL
                     FGRDVVLNTLARQRDTAQPRPVATPAALELAIEPAADCARYDRLRHLPPCQEAYRGAA
                     "
BASE COUNT          489 a          948 c          894 g          443 t
ORIGIN      
        1 cgagcatgac atcgggcagc aagccgccct ttgccttgac gtcggttgct gccagccgct
       61 cattgaggag agcacgacga gcagcgataa aacgctcgaa gtcgacatca accgcaatcg
      121 gtgtcaatca gcgcggaagc acgacccggg atcagcgccc aatgacgacc cacaacaatg
      181 gttgagtagc ggtcgtccac ggagtccacg gaggggaccc gcgcgtgtcg cgcagtgccc
      241 tcacaagcga cggagcgacg cgcgtgggga ggcgctgtgg gcccccgggg gcgatccgcg
      301 gacccgcggc gctgtcaggt tcggtttttg aagcgccagc tctcgttgcc ggtttcgaga
      361 atgtcgcagt gatgggtcag ccggtcgagc agcgccgtgg tcatcttggc gtcggcgaag
      421 acgctaggcc attcggcaaa ggcaagattg gtcgacagca cgatcgaggt gcgttcgtag
      481 agccggctga tcaggtggaa cagcatctgc ccaccgctga ccgggaacgg cagataaccc
      541 agttcgtcga ggatgacgag atcgacgcgg accagctggt cggcgagttt accggacttg
      601 ccggcccgcc cttcggcctc gagctggttg accagatcga cggcggtgaa gaagcgcacg
      661 cgggcgccgt tgcgcacgca attggcgccg atggcgatgg caatatggct cttgccggtg
      721 ccggttccgc caatcagcac ggcgttgcgc tgggtggcga gaaagccgcc ctcgtgtagc
      781 tcgcgcacca gcccctggtt gatcggcgac accgtgaagt cgaactcggt gaggtttttc
      841 atcaccggga agcgcgccac ccccatgcgg taggcggtcg agcgggcgcg atgatcggcc
      901 agctgcgcga gcagcaattc gccgaagatg cgctgaaagg ggtgctgcgc gcgcaccccc
      961 gcggcaacga cctcgtcata ggccgcacgc attccggcca gcttgagctg gcccaatagc
     1021 tccatgatct catgccgctc cacggtacgc ttcctggcat ggcggcaggt ggcgtagccg
     1081 atcgtagcga gcgcagtcgg ccgccggctc gatcgccagc tccaaggctg ccggcgtcgc
     1141 caccgggcgc ggctgcgcgg tgtcacgctg ccgcgcgagg gtgttgagca cgacgtcgcg
     1201 gccgaacagc ttggccgaca gcgcctcggc acaggccgcc tcgaccgcct cgatcccggc
     1261 ctcggccacg gccgtcagga tgtcgacaaa ctggcgatcg ccatcctcat gcccggcaag
     1321 ccggcgccgg gtctgcgcca gtgccggcgg cagatcccag ttgcgaaacg gatcgccgtt
     1381 gcgcagcgcc cccggcttgc gcgccagtac cggcaggtaa tgccagggat cgtaggcggt
     1441 ctggccgcga ccaaagcggc gggaatgttc ggcgacaatc tcgccgtcgc accagacgac
     1501 gatgcgctcg gcgtagacgc gcatctgcgc ggtgcgccgc gccgctttga ccgcgacgct
     1561 gtaacggttg tggtcgtacc ggaccaggct gctcttcgag accgcgacct cgatctcgcg
     1621 gaacccgtca aacggaccgc ggtaagcgat caggctcgcc cgctcggcct cgaacacctc
     1681 ccacaccgtc ttgtcggttt gctcggggtg cgcactcgcg cgcgcctgag cgaggcagcg
     1741 cgcttccagc caagcgttga gttcggcgta gtcggcaaag tgcagccgcg gtgtgaacag
     1801 gtgctcgcgc gcattgccaa cctggttctc gacctgcccc ttctcccagc cagccgccgg
     1861 cgtgcacgcg acgggctcga ccaggtaatg cgagcacatc gccagaaagc ggcggttgaa
     1921 gcggcgttcc ttgccgacaa agacggcttc gaccgccgtc gtcatattgt cgtaaatgcc
     1981 gcgccggcac gccccgccaa acaagcggaa agcgcgctcg tgcgcgtcaa acaccatctc
     2041 ctgggtctcg cgcgggtagg cccgaaccag aaacatccgg ctgtggcaca gccggatatg
     2101 cgcgaccttg accgtggtgg tcaccccacc gagcaccacg acttcgtgcg accagtcgaa
     2161 ctggtacgcc tcgccaggcg caaaccacaa cgggatgaac accgggccct gccgtgattg
     2221 ctgtcggcgc cactcgcgga cgtgacgctg cacactgtca taggcgccct cgtaaccctc
     2281 ggcgcgcagc agctcgaata gccgccgtgc ggtcaggcgc tcgcgcgccg gccgcttgct
     2341 gttcgcctcc agcaatccat caagccgcgc gacataagcc cccagtcgcg gctgcggctg
     2401 aacctgccgc tcgtagcgaa actcgaccga cgaaccccgg atcgccttgc gcaccgtctg
     2461 ccgcgagatc ctcaactcgc gaacgatctg gcggatcggc tttttatcaa cccagaacgc
     2521 ccgcctgatc cggccaatcg tttccactgt taccatctcc ggccacgctc ccccaagcac
     2581 aaccagggag agcttttgat gattcgcggg gcgggtcgct attcgacgct gatcaccccg
     2641 acagccgggt cactattcca cgccgagttc cagcagcgct tcggcgttca catgcgccag
     2701 caccagccgg ggtttcggcc cgaacggcag gccgagcggc acgagccgac cctgttccgg
     2761 gtgcatggct ttcc
//
LOCUS       DATANS010000141         1977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_427943, whole genome shotgun sequence.
ACCESSION   DATANS010000141 DATANS010000000
VERSION     DATANS010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427943"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VHT22_02095"
     CDS             <1..660
                     /locus_tag="VHT22_02095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX3136372.1"
                     /translation="SRETYAGLAHEFRPNLRMIREMMVRPRWMLALLRHGYPRFATIA
                     DYAPPGAWTNEIIAFARARMGGAFSWEEVKRYRDAWKGPMAVKGVLHPEDAAKAVSLG
                     IEGIWVSNHGGRQIEALMPSIDVLPAIAAEVGKKATIVLDSGIRSGQDVMRALALGAD
                     AAFAGKTFLWAVAALGDDGPDYLIDLYIDELRASLGQIGARTIAEAREASIRHPGAVT
                     F"
     gene            783..>1977
                     /locus_tag="VHT22_02100"
     CDS             783..>1977
                     /locus_tag="VHT22_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3136373.1"
                     /translation="MSATAESFQHIKPDDTLARRNAIVLAVAQALAGGNNTVIVSTAS
                     IVGAVLAPDKGLATLPITAMVFGMWLGTLPVGFLARHYGRRFALQCGSAFGILSGFIS
                     YSAVMNGNFWVLLIGTFCGGLYAAAHQSYRFAAADTASDAYRPKVVSWVLAGGVFAAV
                     LGPQLVIFTKDLLSPHLFAASYLGQSVCAILAAAVLQFVKVPPLSPAHYNGARPLLEI
                     VRHSRFIVAVACGIASYATMNMVMTSAPLAMVGCGHSITDAALGIQWHVMAMYAPSFV
                     TGSLIARFGVERITGIGLALIVATAVVGISGVSVAHFWTALVLLGVGWNFAFVGATTM
                     VCDCYRPEERNKVQAFNDFLIFGSMALSSFSSGQLLEKFGWTSINEVIFPAIFVTAAL
                     LLWLSF"
BASE COUNT          293 a          627 c          653 g          404 t
ORIGIN      
        1 tcgcgtgaaa cttatgccgg tctcgcccac gaattcaggc ccaacctgcg catgatccgc
       61 gaaatgatgg tgcggccgcg ctggatgctc gcgctgttgc gccacggcta tcctcgtttt
      121 gcgaccatcg ccgattacgc gccgccgggc gcctggacca acgagatcat cgcgtttgcg
      181 cgcgcgcgca tgggcggcgc cttctcgtgg gaagaagtca aacgctatcg cgacgcgtgg
      241 aaaggcccga tggcggtgaa gggtgtcctt catccggagg atgcggctaa ggctgtgtcg
      301 ctcggcatcg agggcatctg ggtgtcgaac catggcggcc gccagatcga ggcactgatg
      361 ccatccatcg acgtgctgcc ggcgattgcc gccgaagtcg gcaagaaggc gacgatcgtg
      421 ctcgacagcg gcattcgcag tggccaggac gtgatgcggg cgctggcact gggtgccgat
      481 gccgccttcg ccggaaagac gttcctctgg gccgtggcgg cactcggcga tgacgggccg
      541 gactatctga tcgacctcta catcgacgaa ctgcgggcat cgctcggcca gatcggagcc
      601 cggacgatcg ccgaggcgag ggaggcgagc attcgccatc ccggcgcggt gaccttctag
      661 gacacgccgg ccgttcgcac gccccgcaag cggtaagccg cgctcaatcc tgccctgcac
      721 gggaggcatt ggcggtttcc ggggatcgcc gctacaaggg gcggcttccg ggagcccgtt
      781 caatgtcggc gacagccgaa tcgttccagc acatcaagcc cgatgacacg ctcgcacgcc
      841 gcaacgcgat cgtgctcgcg gtcgcgcagg cgcttgccgg tggcaacaac acggtgatcg
      901 tgtccaccgc cagcatcgtc ggcgccgtgc tcgcgcccga caaggggctt gcgaccctgc
      961 cgatcacggc gatggttttc ggcatgtggc tcggcacttt gccggtcggg tttcttgcgc
     1021 ggcactacgg gcggcgcttc gccctgcagt gcggttcggc gttcggcatc ctgtcggggt
     1081 tcatttcata ttcggcggtg atgaacggca atttctgggt gctgctcatc ggcacgttct
     1141 gcggcggcct ctatgcggcg gcacaccagt cctaccgctt cgccgcggcc gatacggcga
     1201 gtgacgccta tcgtccgaag gtcgtgtcgt gggtgctggc gggcggcgtg ttcgctgccg
     1261 tgctcggccc acagctcgtc attttcacca aggatctttt gtcgccgcat ctctttgccg
     1321 cgagttatct gggccagtcg gtttgcgcga tcctcgccgc ggctgtgctg caattcgtca
     1381 aggtgccgcc gctcagcccg gcccactaca acggcgcgcg accgctcctg gagatcgtgc
     1441 gccactcccg cttcatcgtt gccgtggcgt gcggcatcgc gagctacgcc acgatgaaca
     1501 tggtgatgac ttcggcgccg ctggcaatgg tcggttgcgg tcactcgatc accgatgccg
     1561 cgctcggcat tcaatggcat gtgatggcga tgtatgcacc gagtttcgtc acgggctcgc
     1621 tgattgcgcg tttcggcgtc gagcgcatca ccggcattgg tctcgcgctg atcgtcgcga
     1681 cggccgtggt tggaatttcc ggcgtctcgg tggcgcattt ctggaccgcg ctggtgctgc
     1741 tcggcgtcgg ctggaatttc gcgttcgtcg gcgccaccac gatggtgtgc gactgttacc
     1801 gcccggagga acgcaacaag gtgcaggcgt tcaacgattt cctaattttc ggcagcatgg
     1861 cgctgagctc gttctcgtcc ggccagttgc tcgaaaaatt cggctggacg tcgatcaacg
     1921 aagtcatctt cccggcgatc ttcgtgacgg ccgcgctcct gttgtggctg tcgtttc
//
LOCUS       DATANS010000142         4339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_216611, whole genome shotgun sequence.
ACCESSION   DATANS010000142 DATANS010000000
VERSION     DATANS010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216611"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1626
                     /locus_tag="VHT22_02105"
     CDS             148..1626
                     /locus_tag="VHT22_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX3136374.1"
                     /translation="MNACIARIAILIGATALVAACASTDGLSTQAEQHSAAQLSAERS
                     LAGTPAAAPWPQNDWWKSLNDPQLDRLVDEALAGSPSLRVAEARTRQALAVAGIARSQ
                     LFPQINADAELTREHPSKIGETPLPPGSSWSTLHQLEATLSWEIDFWGKNREAYESAL
                     GTARVADIDAYAARLALSVDIAHAYVQLQRAYLQLDIAEATLQQRDQIYALTRDRNAA
                     GVDSLLEVKQAESALPATREQIAQWQESIELSRHQLAALLGQGPDRGLAITRPTASAG
                     ASVALPSALPAELLGRRPDLTAQRLRIEALSHDIASARAEFYPNVNLAAFVGFQSLGA
                     AGFLTSASRMVGFGPAITLPIFDAGRLRSNLAAKNAGYDVAVEQYNQMLADALRDVVD
                     QIASFRSVDEQRAQQALAVATSRQAYDLALLRYREGLGNFLQVLATEAQWLSQQSLDA
                     DLQARRLALSIDLVRALGGGFDPSSVALAGAGRAASRKEPTT"
     gene            1623..1874
                     /locus_tag="VHT22_02110"
     CDS             1623..1874
                     /locus_tag="VHT22_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136375.1"
                     /translation="MSTLEKIPPSRGAGCPPPPGRGRLAVRALAVALLVLLVARGEVV
                     LIKKAVSSGPSGASGTRDGIVRIVDNSLASGLAPIAILP"
     gene            1906..3186
                     /locus_tag="VHT22_02115"
     CDS             1906..3186
                     /locus_tag="VHT22_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3136376.1"
                     /translation="MSEQDLAPQSSALRRERKRWLALAIGVFAVIGIAWGLYWALALR
                     YEQSTDDAYVSGNVVQITPQISGTVVAIGADDTQFVKAGQTLVRLDEADSKVALDQAE
                     AQLARTVREVRSLFATSSQLQSAIAIRQAELATAESDLARRERLSSSGAISGEELQHA
                     REAVSGARAGLSGAQQQFAANRARVDHTTIDEHPDVKNAAAHVRDAYLAYARTTLPAP
                     VSGFVAKRAVQLGQRVSPGAALMAIVPLDQVWVDANFKEPQLASMRVGQPVKLHADIY
                     GGKVVYHGTIAGFGAGTGSAFALLPAQYATGNWIKIVQRVPVRIGLDPEELAAHPLQI
                     GLSMQVEVDTRQRAGDRLPQIGQPAQAVETDVFHSLGELADARVKAIIAANERGAPLP
                     PADLARSTAAPETTGARTLARSAHPAATLNGRPL"
     gene            3196..>4339
                     /locus_tag="VHT22_02120"
     CDS             3196..>4339
                     /locus_tag="VHT22_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX3136377.1"
                     /translation="MTDATLPAAPAVAPAPAAPPAVGPNAAAPPLTGSALVLGTIALS
                     LATFMNVLDTSIANVSIPAIAGDLGVSPDQGTWVITSFGVANAISLPLTGWLTQRFGQ
                     VRLFTFSVLLFVLSSFLCALAPSLGLLILFRVIQGAVAGPMIPLSQSLLLSSYKKERA
                     GTALAMWSITTLVAPVAGPLLGGWITDNISWPWIFYINIPVGIAAAAVTWMIYRPRET
                     ATVRLPIDGVGLGLLVIWVGALQVMLDRGKDLDWFESPQIAILAAVVVVGFVLFLVWE
                     LTERHPVVDLRLFQRRNFWTSTVAMSVAYGTFFGNLVLLPLWLQQYMGYTATMAGMVL
                     APVGLLAILLSPLVGRNITKVDPRVFATVSFAIFAVVLYMRSQFNTD"
BASE COUNT          697 a         1441 c         1448 g          753 t
ORIGIN      
        1 ttcgcttcgc catttcaaat gaaacgccga attgccgtgc aaacgtccga actccactcg
       61 cttttgccgc atcgaactga tgccgggcgg atcgctgcgc gcccggtcgc gcgagtcgca
      121 gcgttatgga cgagcatggg ccatcgcatg aatgcctgca ttgcgcgcat cgcgattctc
      181 atcggcgcaa cagcgctggt cgccgcttgc gcgagcaccg acggactgtc gacgcaagcc
      241 gagcagcaca gcgcagcgca attgtcggcc gagcgctcgc ttgccggcac gccggccgct
      301 gcgccgtggc cgcaaaacga ctggtggaaa tcgctgaacg atccgcaact cgaccggctg
      361 gtcgacgaag cgctggctgg cagtccgagc ttgcgcgtgg ccgaggcgcg cacgcgtcag
      421 gcgctggcag ttgccggcat cgcgcgatcg cagctctttc cgcagatcaa tgccgacgcc
      481 gagctcacgc gcgaacatcc gtccaagatc ggcgagacgc cgcttccgcc cgggagcagc
      541 tggtcgacac tacatcagct cgaggcgacg ctgtcgtggg agatcgattt ctggggcaag
      601 aatcgcgaag cctacgaaag tgccctcggc acggctcgcg tcgccgacat cgacgcttac
      661 gctgcgcgcc ttgcgctgtc ggtcgatatc gcgcacgcct atgttcagct gcagcgcgct
      721 tacctgcaac ttgacatcgc ggaggcgacg ctccagcagc gcgatcagat ctacgcactg
      781 acacgagatc gcaacgccgc cggcgtcgat tcgctgctcg aggtcaagca ggcggagtcc
      841 gcactgccgg cgacgcgcga gcaaatcgcg cagtggcaag aatcgatcga gctctcgcgc
      901 caccagctgg cggcgctgct cggccagggc ccggaccgcg gactggcgat cacgcgaccc
      961 accgccagcg cgggcgcgtc ggtggcgctg ccgtcggcgc tgccggcgga actgctcggc
     1021 cggcgtcccg acctgacggc gcaaaggctc cgcatcgagg cgctgtcgca cgacatcgca
     1081 tcggcccgag ccgagtttta tccgaacgtg aatctcgcgg ctttcgtcgg cttccagagc
     1141 ctgggcgccg ccggattcct gaccagcgcc agtcgcatgg tcggtttcgg ccccgcgatc
     1201 acgctgccga ttttcgatgc cggccggttg cgcagcaatc ttgctgcaaa gaacgccggc
     1261 tacgatgtcg cggtcgagca gtacaaccag atgctggccg atgcgctgcg cgatgtcgtc
     1321 gaccagatcg cgtcgttccg cagcgtcgac gagcagcgtg cccagcaggc gctcgccgtc
     1381 gcgacctccc ggcaggccta tgacctcgcg ctgctgcgct atcgcgaagg cttgggcaat
     1441 ttcctgcagg tgctggcgac cgaggcgcag tggctgtcgc agcaaagcct cgacgcggac
     1501 ttgcaggcgc gccggcttgc gttgtcgatc gatctcgtgc gtgcgctggg cggcgggttc
     1561 gatccatcgt cggtggcgct cgccggtgcc ggccgagcgg caagccgcaa ggaaccaacg
     1621 acatgagcac cctggaaaag atcccgccca gccgcggcgc gggctgcccg ccgccgccgg
     1681 gccggggccg cctagcggtg cgcgcgctcg cggtggcgct gctggtgctg ctggttgcgc
     1741 gcggcgaggt cgtgctgatc aagaaagccg tctcgtcggg gccgagcggc gcgagcggca
     1801 cgcgcgacgg aatcgtgcgc atcgtcgaca acagtctggc cagcggcctc gcgccgatcg
     1861 ccatactgcc ctagagcaac ggcaagtcaa gacagaggaa gcatcatgag tgaacaggat
     1921 ctcgctcctc agagctccgc cctgcgcagg gaacgcaagc gctggctggc gctggccatc
     1981 ggagtctttg ccgtgatcgg catcgcctgg ggactttact gggcgctggc gttgcgctac
     2041 gagcaatcga ccgatgacgc gtatgtcagc ggcaacgttg tgcagatcac gccgcagatt
     2101 tccggcaccg tcgtcgcgat cggcgccgat gatacgcagt tcgtcaaggc cggacagacg
     2161 ctggtgcggc tggacgaggc cgacagcaag gtcgctctcg accaggccga agcccagctt
     2221 gcgcgcaccg tgcgcgaggt gcggagcctt tttgccacca gctctcagtt gcagagcgcg
     2281 atcgccatcc gccaggccga gctcgccacc gccgaatccg atctggcgcg ccgcgagcgc
     2341 ctgtcgagct cgggcgcgat ctcgggcgaa gaactgcagc atgcgcgcga agcggtgtcc
     2401 ggggcgcgcg caggactgtc gggcgcgcag cagcagttcg cagccaaccg tgcccgcgtc
     2461 gatcacacga cgatagacga acatccggac gtcaagaacg ccgcagcaca cgtgcgtgac
     2521 gcgtatcttg cctacgcccg caccacactg ccagcgccag tctccggctt cgtcgccaag
     2581 cgcgcggtgc agctcggaca gcgcgtgagc ccgggagcgg cgctgatggc gatcgtgccg
     2641 ctcgatcagg tctgggttga cgccaatttc aaggagcctc agctcgcttc gatgcgcgtc
     2701 ggacagccgg ttaaactgca cgccgacatc tacggcggca aggtcgtcta tcacggcacg
     2761 attgccggct tcggcgcggg taccggatcg gcgttcgcgc tgttgccggc gcagtacgcg
     2821 accggcaact ggatcaagat cgtgcagcgc gttccggttc gaatcggact cgatccggag
     2881 gagcttgccg cgcatccgct gcagatcggc ctgtcgatgc aggtcgaagt ggacacacgg
     2941 caacgcgcag gcgatcggct gccgcagatt ggtcaaccgg cacaggcggt cgagaccgac
     3001 gtctttcatt cgctcggaga actggccgac gcgcgggtca aggcgatcat cgccgccaac
     3061 gagcgtggcg cgccgctgcc cccggccgat cttgcgcggt cgaccgccgc acccgaaacg
     3121 accggtgcgc gcactcttgc ccgcagtgcc catccggcgg cgacgctgaa cggacgcccg
     3181 ctctaggcac gcgcgatgac ggacgcaaca ctgcctgcgg ccccggccgt tgcaccggcc
     3241 cccgctgcgc ctcccgcggt cggtccgaac gcggctgcgc cgccgctgac cggcagcgcg
     3301 ctggtgctgg gcacgatcgc cctgtcgctg gccacgttca tgaacgtgct cgacacgtcg
     3361 atcgccaacg tgtcgatccc cgcgatcgcc ggcgacctgg gcgtctcgcc tgatcagggc
     3421 acttgggtga tcacttcgtt cggtgtcgcc aacgcgattt cgctgccgct gacgggatgg
     3481 ctgacgcaac gctttggaca agtgcggctg ttcacctttt cggtgctgct gttcgtactg
     3541 tcgtcatttc tttgcgcgct ggcgccttcg ctcgggctgc tgatcctgtt tcgcgtgatc
     3601 cagggcgcgg tcgccgggcc aatgattccg ctgtcgcagt cgctgctgtt gtcgagttac
     3661 aagaaggagc gggcaggcac ggctctggcg atgtggtcga tcacgacgct ggtcgctccg
     3721 gtcgcgggcc cgctgctcgg tggatggatc accgacaaca tctcgtggcc gtggattttc
     3781 tatatcaaca ttccggtcgg catcgcggcc gccgcagtga cctggatgat ctaccgtccg
     3841 cgcgaaacgg cgacggtgcg gctgccgatc gacggcgtgg gattgggtct gctggtgata
     3901 tgggtcggag cgctgcaggt gatgctcgac cgcggcaagg atctcgactg gttcgagtcg
     3961 cctcagatcg cgatcctcgc agccgtcgtc gtcgtcggat ttgtcctgtt cctcgtatgg
     4021 gagctgaccg agcgccatcc tgtcgtcgat ctgcgactgt tccagcggcg gaatttctgg
     4081 accagcacgg tggcaatgtc ggtcgcctac ggaacattct tcggcaatct ggtcttacta
     4141 ccgttgtggc tgcagcaata catggggtac accgcgacga tggcgggcat ggtgctggcg
     4201 ccggtgggac tgctggcgat tctgctgtcg ccactggtcg gaaggaacat caccaaggtc
     4261 gacccgcggg tgttcgcgac cgtgtcgttt gcaatcttcg cggtggtgct ctacatgcgc
     4321 tcgcagttca acaccgacg
//
LOCUS       DATANS010000143         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_91407, whole genome shotgun sequence.
ACCESSION   DATANS010000143 DATANS010000000
VERSION     DATANS010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91407"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VHT22_02125"
     CDS             <1..1135
                     /locus_tag="VHT22_02125"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX3136378.1"
                     /translation="CFALRIASAMGRDQGWLAEHMAILGLTAPDGEKTYVAAAFPSAC
                     GKTNFAMMIPPQGLDRWKVTTIGDDIAWIKPHADGSFHAINPEAGYFGVAPGTSYQSN
                     PNAMETLRENVIFTNVALTDDGDVWWEGMTKDPPAHLTDWRGQSWVPGCGRKAAHPNA
                     RFTVAATQCPSLDPEWDNPAGVPISAFIFGARRSDTVPLVVEATSWDDGVYKAATMGS
                     ETTAAAAGKVGEVRRDPFAMLPFCGYHIGDYFAHWLAMGRAVARPPRVFNVNWFRTDE
                     QGKYAWPGFGQNMRVLKWIIERCQGRAHAVETELGLQPEYGDLDWRGLEFGPERFAQV
                     MRFDRARWTRELDAHDELFAKLGAKQPQALSAQRRALGARFDR"
     gene            1364..>2179
                     /locus_tag="VHT22_02130"
     CDS             1364..>2179
                     /locus_tag="VHT22_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX3136379.1"
                     /translation="MRRDGDVPSLADVARAAKVSTASASRALGRPELVSEAVRTRVSD
                     AAVRLGYVANAAARSLSTRKSGLVGAILSDPADPVVLQMLEAAERSLSAHGVGVLVRA
                     AGAGAPAAACAQALAARGVDGLLFIGAGATPDREGEKSGRSLPYIGCGQTPGSDIAAS
                     GQTIERRGLALACAYLQQLGHHQIGVNRRRQGEGADQRAPSGENATMIEHLVDSFGDT
                     DAVRAAVRWLIENGATAIVASSDVAAAAALRECRALGLDVPRQISVIGCGDSAI"
BASE COUNT          364 a          678 c          763 g          374 t
ORIGIN      
        1 gtgcttcgcg ctgcggatcg cgtcggcgat ggggcgtgac caggggtggc tggccgagca
       61 catggcgatc ctgggcctga ccgcgcccga cggcgagaag acctacgtcg cggccgcgtt
      121 tcccagcgcc tgcggcaaga ccaacttcgc gatgatgatt ccgccgcaag gtctcgaccg
      181 atggaaggtg acgacgatcg gcgacgacat tgcctggatc aagccccacg cggacggcag
      241 ctttcatgcg atcaatcccg aagccggata tttcggtgtc gcgcccggaa cctcctatca
      301 atcgaatccg aacgcgatgg agacgctgcg tgagaacgtc atcttcacca acgtcgcatt
      361 gaccgacgat ggcgatgtct ggtgggaagg catgaccaag gatccgccgg cgcatctgac
      421 cgattggcgc gggcagtcgt gggttcccgg ctgcgggcgc aaagccgcgc atccgaacgc
      481 gcgattcacc gtcgcggcga cgcagtgccc atcgctcgac cccgaatggg acaatcccgc
      541 cggtgtgccg atatcggcat tcatcttcgg tgcgcgccgc agcgacaccg tgccgctggt
      601 ggtcgaggcg accagctggg acgacggcgt gtacaaggcc gcgacgatgg gatcggagac
      661 gaccgcggct gcggccggca aggtcggcga agtgcggcgc gatccgttcg cgatgctgcc
      721 cttctgcggc tatcacatcg gcgactattt cgcgcattgg ctggcgatgg gtcgtgcggt
      781 agcgagaccg ccgcgcgtgt tcaacgtcaa ctggtttcgc accgacgagc agggcaaata
      841 cgcatggccg ggattcggcc agaacatgcg cgtgctgaag tggatcatcg agcgctgcca
      901 gggtcgcgca cacgcggtcg aaaccgagct cggcctgcaa cccgagtatg gcgatctcga
      961 ttggcgcggc ctcgagttcg gtcccgagcg gttcgcgcag gtgatgcgat tcgaccgcgc
     1021 gcgctggacg cgtgagctcg atgctcacga cgagctgttc gcaaaattgg gcgccaagca
     1081 gccgcaggcg ctttcagcgc agcggcgggc gcttggcgcg aggttcgatc gctaagcgtc
     1141 tgcaaatttc cgaatgaagg gcgcagttcg cgcccttttt ctttgggcgc ggcgcttgcg
     1201 cattccagac tcagcggcta atcttgaagg attgcaaccg ctttctgata tcgattgcag
     1261 aaagcgatta ctgtaatcga ttgcgatgcc ttcagaaacc attcccgcgc gtcccgacgc
     1321 agccgttcaa gacggaacgc aagctgtccc gtcgcggcgg cgaatgcggc gggacggcga
     1381 tgtgcccagt ctggcggatg tagcccgtgc ggcgaaggtt tcgacggcca gcgcctcgcg
     1441 tgcactgggg cgccctgagc tcgtcagcga ggcggtgcgg acccgcgttt cggacgccgc
     1501 ggtgcggctc ggctacgttg ccaatgccgc cgcgcgttcc ttgtcgacgc gcaagtcggg
     1561 gctggtgggg gcgatcctca gcgatccggc cgatccggtc gtcttgcaga tgctggaggc
     1621 ggccgagcga agcctctccg cacacggagt cggcgtgttg gttcgcgcgg cgggcgcggg
     1681 ggcgccggcc gccgcgtgcg cgcaggcgtt ggcggcacga ggcgtcgatg gcctgctgtt
     1741 cattggcgcg ggcgcaacgc cggaccggga gggcgagaag tctggccgct cccttccgta
     1801 catcggatgc gggcaaacgc ccggatcgga tattgcagcc tccggccaga cgatcgagcg
     1861 gcgcggcctg gccttggcct gcgcgtatct gcagcaattg ggacatcacc aaatcggcgt
     1921 caacaggcgg cgccagggcg agggcgccga tcagcgtgcg ccatcgggag aaaacgcaac
     1981 aatgatcgaa cacctggtcg attcgttcgg tgacactgac gctgtccggg ccgcggttcg
     2041 ctggctgatc gaaaacggcg ccacggccat cgttgcgtct tccgacgtcg cggcagccgc
     2101 tgcgctgcgc gaatgccgcg cgctgggcct cgacgtgcca cggcagatct cggtgatagg
     2161 ttgtggcgat agcgcgatc
//
LOCUS       DATANS010000144         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_216837, whole genome shotgun sequence.
ACCESSION   DATANS010000144 DATANS010000000
VERSION     DATANS010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216837"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /gene="phnY"
                     /locus_tag="VHT22_02135"
     CDS             complement(<1..1391)
                     /gene="phnY"
                     /locus_tag="VHT22_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde dehydrogenase"
                     /protein_id="HEX3136380.1"
                     /translation="MNARDIRHESMRIAGDLVGGDRVIEVRNPYTGAVVGTVPKASVE
                     DIRRAFAIARAYRPTLTRFERSRILHRAAEIIRGRTDELSDLITAECGICKKDSLYEV
                     GRACDVFVFAGNAALSDDGQIFSCDLTPHGKRRKVYTLREPLLGVISAITPFNHPLNQ
                     VAHKVAPAIATNNRMVLKPTEKTPLSALVLADILYEAGLPPPMFSVVTGDPKEIADEM
                     LTNADVDLVTFTGGIAVGKYIAAKAVYKRQVLELGGNDPLIVMEDADIDEAATLAAAG
                     SYKNSGQRCTAIKRMLVQQSIADRFVELLLEKTRAVKYGDPMDPATDMGTVIDEEAAK
                     SFEGKVDDAVARGARLLYGNVRRGALYSPTVLDRVAPDMPVVKYETFGPVSPVIRFDT
                     IDQAIAIANSTDYGLSSAVCTNRLDYITRLVSELNVGSVNVREVPGYRLELTPFGGIK
                     DSGLGYKEGVQEA"
     gene            complement(1388..>2267)
                     /locus_tag="VHT22_02140"
     CDS             complement(1388..>2267)
                     /locus_tag="VHT22_02140"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX3136381.1"
                     /translation="QGDILVAVGGGSVLDTAKALMVGTADGSFEALIALLATGKPFAP
                     HRIKPLIAIPTTAGTGSEVTPWATIWDRGAGKKYSLHLRETWAEAALVDPALTVSLPA
                     GATLAAGLDALSHSLESIWNVNANPVSDTHAVTAARAVLATLPALVGDLDNLELRRRM
                     SLAALQAGLAFSNTKTALAHSISYDMTLRHGLPHGIACSFTLPLVLARAVGADAGRDA
                     VLARIFDGALADAPARLTAFLEALGVSTRFETYGVSEAQAAGMIEAALDGVRGRNFIG
                     AGRAASLRPSMEHAAS"
BASE COUNT          378 a          736 c          770 g          383 t
ORIGIN      
        1 atcgcttcct gcacgccttc cttgtatccg agcccggaat ctttgatgcc tccgaaaggg
       61 gtaagttcga gacggtagcc gggcacctcg cgcacgttga cgctgccgac attgagctcg
      121 gacaccaggc gcgtgatgta gtcgagccgg ttcgtgcaca ccgccgacga cagtccgtag
      181 tcggtcgaat tggcgatcgc gatcgcctgg tcgatcgtgt cgaagcgaat caccggcgaa
      241 accggcccga aggtctcgta tttcaccacc ggcatgtcgg gcgctacccg gtctagcacc
      301 gtcggcgaat agagcgcgcc gcggcgcacg ttgccgtaga gcaatcgcgc gccgcgcgcg
      361 accgcgtcgt cgaccttgcc ctcgaaggac ttcgccgcct cctcgtcgat caccgtgccc
      421 atgtcggtcg ccggatccat cgggtcgccg tacttgacgg cccgggtctt ctccagcaga
      481 agctcgacga accggtcggc aatcgattgc tggaccagca tgcgcttgat cgccgtgcag
      541 cgctggcccg agttcttgta cgagcctgca gcagccagcg tcgcggcttc gtcgatgtcg
      601 gcgtcttcca tgacgatcag cgggtcgttg ccgccgagct ccagcacctg gcgcttgtaa
      661 acggccttgg ccgcgatgta cttgccgacc gcgatgccgc cggtgaaagt caccagatcg
      721 acatcggcgt tggtcagcat ctcgtcggcg atctccttcg ggtcgccggt cacgaccgaa
      781 aacatcggcg gcggcaaacc ggcttcgtac aggatatcgg ccagcaccag ggcggaaagc
      841 ggcgtctttt cggttggttt cagcaccatg cggttattgg tcgcgattgc cggagcgact
      901 ttgtgcgcga cctggttcag cggatggttg aacggggtga ttgcggagat gacgcccagc
      961 agcggctcgc gcagcgtgta gaccttgcgc cgcttgccgt gcggcgtcag atcgcaggaa
     1021 aagatctggc cgtcgtcgga cagcgccgca ttaccggcga acacgaatac gtcgcaggcg
     1081 cggccgacct cgtagagcga gtccttcttg cagatgccgc actcggcggt gatcaggtca
     1141 gacaactcgt cggtccggcc gcggatgatc tcggcggcgc gatgcaggat ccgcgagcgc
     1201 tcgaatcgcg tcagcgtcgg tcggtaggca cgcgcgatcg cgaaggcccg gcgtatgtcc
     1261 tcgacgctgg ccttgggcac ggtgccgaca accgcgccgg tgtacggatt gcgcacctcg
     1321 atgacccggt cgccaccgac cagatcgccg gcgatgcgca tcgattcgtg gcggatgtca
     1381 cgtgcgttca tgatgcggcg tgctccatcg aaggtcgcag cgaggcggcg cgtcccgcgc
     1441 cgatgaaatt cctgccgcga acgccgtcca gcgcggcttc gatcatgccg gcggcttgcg
     1501 cctcggacac gccataagtt tcgaagcgcg tgctgacgcc cagggcttcg agaaacgcgg
     1561 tgagccgggc gggagcatca gccagcgcgc catcgaatat acgtgcgagc acggcatcgc
     1621 gcccggcgtc ggcgccgacg gcgcgggcca gcacgagcgg caaggtgaac gagcaggcga
     1681 tgccatgcgg cagcccgtgc cgcagggtca tgtcatagga aatcgaatgc gcgagcgcgg
     1741 tcttggtgtt ggaaaaagcg agtccggcct gcagcgcggc aagcgacatg cgacgacgca
     1801 gctcgagatt gtcgagatcg ccgaccagcg cgggcagcgt cgcgagcacc gcgcgcgccg
     1861 ccgtcaccgc gtgcgtatcc gagaccggat tggcgttgac attccagatc gattcgagcg
     1921 agtgcgaaag agcatcgagg cccgcggcaa gcgtggcgcc cgccggcagc gacacggtca
     1981 gggcgggatc gaccagcgcc gcttcggccc aggtctccct gaggtgcagc gaatacttct
     2041 tgccggcgcc gcggtcccag atcgtcgccc agggcgtgac ctcgctgccg gtgccggcgg
     2101 tggtcggaat ggcgatcagc ggcttgatcc ggtgcggcgc gaacggcttg ccggtggcca
     2161 gcagcgcgat cagcgcttcg aagcttccgt cggcggtgcc caccatcagc gccttggcgg
     2221 tatcgagcac gctgccgccg ccgaccgcga cgaggatgtc tccctgg
//
LOCUS       DATANS010000145         1858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_804156, whole genome shotgun sequence.
ACCESSION   DATANS010000145 DATANS010000000
VERSION     DATANS010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804156"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VHT22_02145"
     CDS             complement(<1..845)
                     /locus_tag="VHT22_02145"
                     /inference="COORDINATES: protein motif:HMM:NF045284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEX3136382.1"
                     /translation="MTVSAEFGRAQHLEEVLKLVQAKVPAAQRNTIAAFVQRYYGQVD
                     PEDLAERAPADLYGAALSHWNFARRRDPAHARVRAFNPSIEEHGWQSTHTIIEIVNDD
                     MPFLVDSVTMEVNRHGLTLHLIIHPIVAVVRDADGTLAGVADDTEGAQRESMIHVEVD
                     RIVDPAKLDELAADIVRVLDDVRAAFEDWKKMRDRVGAILVENEKRAPPLPPDELAEG
                     RAFLSWLADDHFTFLGYRRHELVVIGGNDALKIVPGSSLGILREGENKEVATSFAALP
                     PEVKA"
     gene            complement(902..988)
                     /locus_tag="VHT22_02150"
     tRNA            complement(902..988)
                     /locus_tag="VHT22_02150"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(952..954),aa:Leu,seq:caa)
     gene            1132..>1858
                     /locus_tag="VHT22_02155"
     CDS             1132..>1858
                     /locus_tag="VHT22_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEX3136383.1"
                     /translation="MTTRKPYRLSDFDFDLPSELIAQVPAPERSGSRLLHVAGARLVD
                     LVFGALPELLVPGDLLVFNDTRVVRSRIVGRKHSGGRVELMLERIVAPREAWMQLRAS
                     HMPRVGATIELPGNASATVCERNERFFRLRFDLPTTLATYLEQHGAVPLPPYIARAPE
                     PADAERYQTVYARHPGAVAAPTAGLHFDQALLAALHARKIGFAYVTLHVGAGTFLPVE
                     SDDLSQHRMHSERFSIPRATSAAI"
BASE COUNT          304 a          623 c          600 g          331 t
ORIGIN      
        1 tacgccttga cctcgggcgg cagcgccgca aagctggtcg cgacttcctt gttctcgcct
       61 tcgcgcaata tgccaaggct cgagccggga acgattttca gcgcatcgtt cccgccgatg
      121 acgacgagct cgtggcgccg gtagccgaga aaggtgaagt gatcatcggc gagccacgac
      181 aggaaagccc ggccctcggc gagctcgtcc ggcggcagcg gcggcgcccg tttctcgttc
      241 tccacgagta tcgcgccgac gcggtcacgc atctttttcc agtcctcgaa cgccgcccgc
      301 acgtcgtcca gcacgcggac gatgtcggcg gcaagctcgt cgagctttgc aggatcgacg
      361 atgcgatcga cctcgacatg gatcatcgac tcgcgctgcg cgccctcggt gtcgtcggcg
      421 acgccggcca atgtgccgtc ggcgtcgcgc acgacggcga cgatcgggtg gatgatcagg
      481 tgcagggtca gcccgtgccg attgacctcc atcgtcaccg agtcgaccag gaacggcatg
      541 tcgtcattca cgatctcgat gatcgtgtgc gtcgactgcc agccatgctc ctcgatcgat
      601 ggattgaagg ccctgacgcg agcgtgcgcc ggatctcggc ggcgggcgaa attccagtgc
      661 gagagcgcgg ccccgtacag atcggcggga gcccgttcgg cgaggtcctc gggatcgacc
      721 tgaccgtagt aacgctggac gaaggcagcg atggtgttgc gctgcgcggc cggaaccttc
      781 gcctgtacca gcttgagcac ctcctcgaga tgctgtgccc ggccgaattc ggcggatact
      841 gtcacgggga cctccacgcc gagatcatgc gccttcggcg gcatgaccgg cgctcatatt
      901 ttggtgccgg gagggggaat cgaaccccca ctctgttgcc agaactggat tttgagtcca
      961 gcgcgtctac caattccgcc atcccggccg gccgcctgca tcgaatccgc cccgacaggc
     1021 caaaggcgac gcgcaacgcg cgggccgcgt tgccggcgcg cggtgaagcc ggcattatcg
     1081 cggaaagggc gcgccgcttc aaaccgcgga catctttgaa cgcgccgcac gatgacaacc
     1141 cgcaagccct accgcctttc cgatttcgat ttcgatctgc cgagcgagct gatcgcgcaa
     1201 gtccctgcgc cggagcgttc cggaagccgc ttgctgcacg tcgcaggggc gcggcttgtc
     1261 gatcttgtgt ttggagcctt gcccgagctg ctggttccgg gcgatctcct ggtcttcaac
     1321 gatacccggg tcgtgcgttc gcgcatcgtc ggccgcaagc acagcggcgg cagggtcgag
     1381 ctgatgctcg agcgcatcgt cgcgccgcgc gaggcctgga tgcagttaag ggccagtcat
     1441 atgccccgcg tcggcgcgac gatcgagctc cctggaaacg catcggcgac ggtgtgcgag
     1501 cgcaacgagc gcttcttccg actgcgcttc gacctgccga cgacgctcgc cacctatctc
     1561 gagcagcatg gcgcggtgcc gctgcctcca tacatcgcga gagcaccgga accggccgat
     1621 gcggagcgct atcagaccgt gtatgcccgc catcctggcg ccgtggcggc gccgaccgcg
     1681 gggctgcatt ttgatcaggc cttgctcgcc gcgctacacg cccggaagat cggtttcgct
     1741 tacgttacgc tccacgtcgg cgctggaacg ttcctcccgg tggaaagcga cgacctgtcg
     1801 cagcatcgga tgcacagcga gcgcttcagc attccgcggg cgacatcggc tgcgatcg
//
LOCUS       DATANS010000146         1880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_763018, whole genome shotgun sequence.
ACCESSION   DATANS010000146 DATANS010000000
VERSION     DATANS010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763018"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VHT22_02160"
     CDS             complement(<1..736)
                     /locus_tag="VHT22_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX3136384.1"
                     /translation="MSGSEGLWMLGTVAAALVATGLPAWIVLIGVTLGFAAGGVVTDA
                     LQMSLLTAMPARILGLLEHDLLQALPLYVLMGALLNHLPLAQTLFRAGSRALAPIGAN
                     ASLAGLGLGVLLAPMNGSVGASAAMLSRTVQPRLDQSGMTPAKSAALVCVASTMGVVI
                     PPSLVLILLGDAMMRAHTEAVNITGAAIRIINTQDVFAGALVPAGILLAFCIAITWWD
                     ARNATPAPTSAVTARDWLVAAITAAFI"
     gene            complement(733..1248)
                     /locus_tag="VHT22_02165"
     CDS             complement(733..1248)
                     /locus_tag="VHT22_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX3136385.1"
                     /translation="MPTDDTGSSRSALRSLDRLIQQTTTGASILVLPVSLLLFLQWPL
                     RDLGIGYSREANDLAQTLFALYVAVAITDATRRNSHLAADAFALRYPPRLRRLLMRIA
                     SVVVLAPWALFIVYASWPMVVQSVRQLESFPETYDPGYFIVKIALLLLALLILLQVIL
                     DAIRDGQATTQ"
     gene            complement(1332..1727)
                     /locus_tag="VHT22_02170"
     CDS             complement(1332..1727)
                     /locus_tag="VHT22_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136386.1"
                     /translation="MTTHSYRILLGASLALLVGAAGAQNAPDNNSRMPAAKGAITTMP
                     AQPSEQPTDKMAADPNATSQGTAAPKSDATQPAPAHRVQKSAPRQQEAVSPEEKSYRE
                     ALRQCAQERNESQRDACIDNAIGQHQPNG"
BASE COUNT          341 a          608 c          618 g          313 t
ORIGIN      
        1 cgatgaacgc cgcggtgatg gccgccacca gccaatcgcg tgccgtgacc gccgacgtcg
       61 gcgccggcgt ggcattccgc gcatcccacc acgtgatcgc gatgcagaac gcgagcagga
      121 ttccggccgg cacaagcgca cccgcgaaga catcctgcgt gttgatgatg cggatcgccg
      181 cgccggtgat gttcaccgct tcggtgtgtg cacgcatcat cgcatcgccc aaaaggatca
      241 gcaccagcga gggcggaatg acgacgccca tcgtgctggc cacgcagacc agcgccgcgc
      301 ttttcgccgg cgtcatgccg ctctgatcca gccgcggctg cacggtgcgg gaaagcatcg
      361 ccgcgctggc gccgaccgaa ccattcatcg gcgccagcag cacgcccagc cccagccccg
      421 ccagagaagc attcgcaccg atcggcgcga gcgcgcggct gccggcgcga aacagcgtct
      481 gcgcgagcgg caggtggttg agcagcgctc ccatcagcac atacagcggc agcgcctgca
      541 gcaaatcgtg ctcgagcagg ccgagaatcc gcgcgggcat cgcggtcagc agcgacattt
      601 gcagcgcgtc cgttacgacg ccaccggcgg caaagcctag cgtcacgccg atcagcacga
      661 tccaggcggg cagtccggtc gcgaccagcg cggccgcgac cgtgccgagc atccaaagac
      721 cttcgcttcc gctcattgcg tcgtcgcctg gccgtcgcga atcgcatcga ggatcacctg
      781 cagaaggatc agcagcgcga gaagcagcaa tgcgatcttg acgatgaaat agccggggtc
      841 gtacgtttcg ggaaaggatt cgagctgccg aaccgactgc actaccatcg gccaggacgc
      901 gtagacgatg aagagcgccc atggcgcaag cacgacgacg gaggcgatac gcatcagcag
      961 gcggcgcagc cgcggcggat agcgcagcgc aaaggcgtcg gcggcaagat gactgttacg
     1021 gcgcgtcgcg tcggtgatcg ccacggcgac gtacagcgcg aacagcgtct gcgcgagatc
     1081 gttcgcctcg cgcgagtatc cgattcccag atcgcgcaac ggccactgca gaaacagaag
     1141 caacgaaacc ggcagcacga gtatcgaagc gccggtcgtc gtctgctgga tcaggcggtc
     1201 gagcgagcgc agcgcggagc gggaactccc ggtatcgtcg gtgggcatcg tcggcagctg
     1261 ggcgcgcctc gataatagcg caggtcaaaa aaaaccccgc ctgggcgggg ttttttgcga
     1321 actcttgtgc gtcagccgtt cggctggtgc tggccgatcg cgttatcaat acaggcgtcg
     1381 cgctgcgact cgttgcgctc ctgcgcgcat tggcgcaatg cctcacggta gcttttctcc
     1441 tcgggagaca cggcttcttg ctgacgagga gctgactttt gcacgcgatg cgcaggggcg
     1501 ggctgtgtgg cgtccgactt cggcgccgcc gtgccttgtg aggtggcgtt tggatctgct
     1561 gccatcttgt cggtcggctg ctccgacggc tgtgccggca tcgtggtgat cgcgcctttt
     1621 gccgctggca tgcgcgagtt gttgtccggc gcgttttgcg cgccggctgc gccgacgagc
     1681 agtgccaggc tcgcgcccag caggattcgg tatgagtgag tggtcatcgg gttctccttc
     1741 gaaggttggc tttttgcgcc gatctgacgc cggttcctga caacgaaccg cccgattaac
     1801 tatccgccaa aatcccgccg atcaatatcg gaccgcttct gattcgcacg taggagcggg
     1861 ggatacccat cccggactcg
//
LOCUS       DATANS010000147         1850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_721486, whole genome shotgun sequence.
ACCESSION   DATANS010000147 DATANS010000000
VERSION     DATANS010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721486"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1701)
                     /locus_tag="VHT22_02175"
     CDS             complement(<1..1701)
                     /locus_tag="VHT22_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HEX3136387.1"
                     /translation="MTRQSQYARFGSGKSVPRVEDRALLTGAGRFADDFSVPGQACVC
                     FLRSPHAHARIATIDTRAAAGMPGVIAIVTGEDLVRDKVAPLPLSADFKRADGSPTAS
                     PPRHALAVGTVRFVGEAVAAAIAQSAQQARDAAEAIDIHYDPLPAVTDAAAAVAAGAP
                     QVWPAASGNVACEARHGDAAAAGAAFARAVHRVSLDLVNQRVAPCPIEPRAVLASYDA
                     TTERITLRVGCQTPTGLRDDLCQVVLGIPTDKVRVLVGDVGGGFGMKTGLYPEDVVLA
                     WGARKYGVPLKWRAERIEEFLAATHGRDISSKAELALDGDGKVLALRIDSLANVGACG
                     TPAGVVIQLMIGPWVSTSVYDIGTIDIRIRAVLTNTAPTGAYRGAGRPEAIYIIERLM
                     DAAARQAGIDPVELRRRNMIRPEQMPYRNAMNKTYDSGQFELVLDKATTLAGWKDFDA
                     RAADSHRRGRLRGRGMAAFLEWTGVDIFEERVTVTVEGSTNGQGTIEIFSATQAMGQG
                     LATTFAQLAVDVFGVPIDSIRIVQGDTDRGTGFGSAGSRSLFVGGSAVKVGAERTVDA
                     A"
BASE COUNT          295 a          660 c          622 g          273 t
ORIGIN      
        1 cgcggcgtcg accgtgcgct cggcgcccac cttcaccgcc gagcccccga cgaacagcga
       61 gcgcgatccc gcgctgccga aaccggtgcc gcggtcggtg tcgccctgca cgatgcgaat
      121 cgaatcgatc ggcacgccga acacgtcgac ggcgagctgc gcgaaagtcg tcgcgagtcc
      181 ctgtcccatc gcctgcgttg ccgagaagat ctctatcgtg ccctggccgt tcgtcgaccc
      241 ttcgacggtg accgtcacgc gttcctcgaa tatgtcgacg ccggtccatt cgagaaaggc
      301 cgccatgccg cgcccgcgca agcggccgcg ccgatgcgaa tcggcggccc gcgcgtcgaa
      361 atccttccag ccggcgagcg tcgtcgcctt gtcgagcacc agctcgaact gcccgctgtc
      421 gtaggtcttg ttcatcgcgt tgcgatacgg catctgctcg gggcgaatca tgttgcgccg
      481 acgcagctcg accggatcga tgccggcctg gcgcgcggcg gcgtccatca ggcgctcgat
      541 gatgtagatc gcctcagggc gtccggcgcc gcgataggcg ccggtcggtg cggtgttcgt
      601 gagcaccgcc cgaatgcgga tgtcgatcgt cccgatgtcg taaacgctgg tcgataccca
      661 ggggccgatc atcagctgga tgacgacgcc ggccggcgtc ccgcaggcgc cgacgttggc
      721 gagcgaatcg atgcggagcg cgagcacctt gccgtcgccg tccagcgcga gctccgcctt
      781 gctgcttata tcgcggccgt gcgtcgcggc caggaattcc tcgatacgtt ccgcgcgcca
      841 tttgagcggt accccgtatt tgcgggcgcc ccaggcaagc acgacatctt ccggatagag
      901 gccggtcttc atgccgaagc cgccgccgac gtcgccgacc agcaccctca ccttgtccgt
      961 aggaatgccg agcacgacct ggcagaggtc gtcgcgcaac cccgtcggcg tctggcaacc
     1021 gacgcgcagc gtgatccgct cggtcgtcgc gtcgtagctt gcgagcaccg cgcgcggctc
     1081 gatcggacac ggggccacgc gctgattgac cagatcgagc gatacgcggt gcaccgcccg
     1141 ggcgaacgcc gcgccagcgg cggccgcatc gccatgccgc gcttcgcacg cgacgttgcc
     1201 ggaggcggcg ggccagacct gcggcgcgcc ggcggccacc gcagcggcgg cgtcggtcac
     1261 cgcaggcagc gggtcgtagt ggatgtcgat cgcctcggcc gcatcgcgtg cctgctgcgc
     1321 gctttgcgcg atcgccgcgg ctaccgcttc gccgacaaaa cgcaccgttc cgacggccag
     1381 cgcgtgtcgc ggcggcgacg cggtcggcga tccgtcggcg cgcttgaagt cggccgacag
     1441 cggcaacggc gcgaccttat cgcgcaccag gtcctcgccg gtcacgatcg cgatcacgcc
     1501 cggcatgccg gcagcagcac gggtgtcgat cgtcgcgatg cgcgcatgcg cgtgcggtga
     1561 acgcaggaag cagacgcacg cctggcccgg aacggagaag tcgtcggcga aacgaccggc
     1621 accggtcaac agcgcccggt cctcgacgcg cggcaccgac ttgccactgc cgaagcgcgc
     1681 atactgggat tgtctggtca cggcgaaaac ctcgagagcg ggggaaagcg gaatccgtcc
     1741 aagccgagat ggtaacgcgc cgcgccgctc ggcgcccaat gagagcgaag cttgaccacc
     1801 cgagcgggtg gccacacaat ccgcggaaat catcagcgca cgcgaccttc
//
LOCUS       DATANS010000148         2306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_637901, whole genome shotgun sequence.
ACCESSION   DATANS010000148 DATANS010000000
VERSION     DATANS010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637901"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1088)
                     /gene="pip"
                     /locus_tag="VHT22_02180"
     CDS             complement(138..1088)
                     /gene="pip"
                     /locus_tag="VHT22_02180"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEX3136388.1"
                     /translation="MSRDTFLYPPLAASRTGRLKLDALHTMYWEESGNPRGVPVVFLH
                     GGPGGGSSPDHRRFYDPAHYRIVVYDQRGAGQSTPLGELRDNTTPLLIADIERLREHL
                     GIERWLLFGGSWGSTLALAYAETHAERVLGLVLRGIFLCRPQEIHWFLYQIRFMQPEA
                     WRTFAEYLPASERGDLLAGYHRRLIDPDPAVHMPAAHRWSRYEGSCSTLLPDPDLVSH
                     FDEDAVALAIARIEAHYFVNRIFLPDNALLAGIDRIRNLRCTIVQGRYDLVCPIFSAD
                     ELHRAWPEAEYIVVGDAGHSAREPGIARELVAATDRFRGR"
     gene            complement(1137..1997)
                     /locus_tag="VHT22_02185"
     CDS             complement(1137..1997)
                     /locus_tag="VHT22_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX3136389.1"
                     /translation="MKLVRYGPAGKEKPGLIDGDGKLRDLSRRIRDIDGTTLAPAKLK
                     ELAKLDTRRLPLVKGKPRLGACVATPAKFVAIGLNYIDHAKETGSPIPEHPIVFFKSQ
                     TCIVGPNDNVMLPPDSVSTDWEVELGVVISRTARHVTEKDALKYVAGYCIVNDVSERE
                     FQMKRSGSQWSKGKGCDTFGPIGPYLATTDEIKNPQNLDMWLDVNGVRRQTGNTRTMI
                     FGIAALVADVSRYMTLLPGDLITTGTPPGVGLGMKPTPQYLKAGDVVTLGIQGLGEQR
                     QKIVSFKKGR"
     gene            complement(2106..>2306)
                     /locus_tag="VHT22_02190"
     CDS             complement(2106..>2306)
                     /locus_tag="VHT22_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3136390.1"
                     /translation="VDTPSLHERINAFPDPVEARRNFIARQPMGRLATPEEIAPVVVF
                     LASDESAFATGTVYSVDGGMTI"
BASE COUNT          401 a          724 c          772 g          409 t
ORIGIN      
        1 ttgtcggcga cgacatcggg ctaacgttcc aggtcaccgc ttttcgcgtt ccgcacgacg
       61 gggagagcga cccgccctag cgacaatcag ccgaggcgat gccgatggcg cgagcgccaa
      121 tgcctacccc gcgcgcgcta gcgaccgcgg aatcgatcgg tcgccgccac cagctcgcgc
      181 gcgatgccgg gctcgcgcgc cgaatggccg gcgtctccga cgacgatgta ttcggcttcc
      241 ggccaagcgc gatgcagctc atcggcggaa aagatcgggc agaccaggtc gtagcggccc
      301 tggacgatcg tgcagcgcag gttgcgaatg cgatcgatgc cggcgagcag cgcgttgtcc
      361 ggcagaaaga tccggttgac aaagtaatgc gcctcgatgc gcgcgatcgc cagcgcgacc
      421 gcatcctcgt cgaaatgcga gaccaggtcg ggatcgggaa ggagagtcga gcacgagcct
      481 tcgtagcggc tccagcgatg cgctgccggc atgtgcactg ccggatcggg atcgatcagc
      541 cgacgatgat agccggcaag cagatcgccg cgctcggacg ccggcaggta ttcggcgaag
      601 gttcgccagg cttcgggctg catgaagcgg atctggtaga gaaaccagtg aatctcctgc
      661 gggcggcaga ggaaaatgcc gcgcagaacc agtccaagca ctcgctccgc gtgcgtttcg
      721 gcgtaggcca gtgcaagggt cgagccccaa gagccgccga acagaagcca gcgctcgatg
      781 cccaagtgct cgcgcagccg ctcgatatcg gcgatcagca gcggcgtcgt gttgtcgcgc
      841 agctcgccca gcggggtcga ctgaccggcg cctcgctggt cgtagacgac gatccggtag
      901 tgcgccggat cgtaaaagcg acggtggtcg ggtgagctgc cgccgccggg cccgccatga
      961 aggaagacga ccggcacacc gcgcggattg cccgactctt cccaatacat cgtatgcagc
     1021 gcgtcgagct tcagacgacc ggtgcggctc gccgctagcg gcggatacag gaaggtgtcg
     1081 cgcgacagcc cgcgcgtggc gtcgttgccg gtcacgatct tgccggagcg agcctcctaa
     1141 cgacccttct tgaaggagac gattttctgg cgctgctcgc ccagtccctg aatgccgagg
     1201 gtgacgacat cgccggcttt cagatactgc ggcgtcggct tcatgcccag gcccacgccg
     1261 ggcggcgtgc cggtggtgat gaggtcgccc gggagcagcg tcatgtagcg cgagacgtcg
     1321 gcgaccagcg cggcgatccc gaaaatcatc gtccgggtgt tgccggtctg gcgccgcaca
     1381 ccgttgacgt cgagccacat atccagattc tgcgggttct tgatttcgtc cgtcgttgcc
     1441 aggtacggac cgatcgggcc gaacgtgtcg cagcctttgc ccttgctcca ttgcgagccg
     1501 ctgcgcttca tctggaactc gcgctcggat acgtcgttga cgatgcagta tccggcaacg
     1561 tatttcaacg catccttctc ggtgacatgc cgcgcggtgc gcgagatgac gacgccaagc
     1621 tcgacctccc agtcggtgct caccgaatcc ggcggcagca tcacgttgtc gttcgggccg
     1681 acgatgcagg tctgcgattt gaagaagacg atcggatgct cggggatcgg cgagccggtt
     1741 tccttggcgt gatcgatgta gttgaggccg atggcgacga acttcgccgg cgtcgcgacg
     1801 caagcaccca aacgcggctt gcccttgacc agcggaaggc gccgggtgtc gagcttcgcc
     1861 agttccttca gcttcgccgg agcgagcgtc gttccgtcga tgtcgcggat cctgcgcgac
     1921 agatcgcgca gcttgccgtc gccatcgatc agtccgggtt tttccttgcc tgcggggcca
     1981 tagcgtacga gcttcatgtt cgggctttca tcgaaatccg tctctcgaca ctcgagcgcg
     2041 gatggggttg atgcgccgcg caggccgagc tggtcgaagc attgcgcgga ggttggaatt
     2101 ctatttcaaa tcgtcatgcc gccgtcgacg gagtagacgg tcccggtcgc gaacgccgat
     2161 tcgtccgacg ccaggaacac gacgaccggc gcgatttcct cgggtgtggc gagtctgccc
     2221 atcggctggc gcgcgatgaa gttcctgcgc gcttcgaccg gatccggaaa ggcgttgatg
     2281 cgctcgtgca gcgacggcgt gtcgac
//
LOCUS       DATANS010000149         2666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_7278, whole genome shotgun sequence.
ACCESSION   DATANS010000149 DATANS010000000
VERSION     DATANS010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7278"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1116
                     /locus_tag="VHT22_02195"
     CDS             157..1116
                     /locus_tag="VHT22_02195"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX3136391.1"
                     /translation="MADTQPKLVKVTNQREIPIKPDMVLGRQAECDLQLTEGHASRKH
                     AKLLIADDGVWLEDLNSANGTFVNGAKVTGKVKLSSGDRLRFDVEEFDFRLPSAAPPV
                     DDGKTQYREPASAAVDAASSGLYKRPGAWADPDAMGDGANKTKFIDPAQLKQMVNAPA
                     AAAVNTASIDGPHLHVASGSRAGLNIKLTVGESGVREWTIGSQADREVQFQDSGVSAL
                     HAKIVNEGERWKVLDQMSANGTYVNGKRSNVSYLTAGDRVRFGPVECIFQASRAGAAP
                     RRSASPSASSEAGSESKNKTLLIAGIAFAVTIVVLFLVYKFVL"
     gene            1124..2257
                     /locus_tag="VHT22_02200"
     CDS             1124..2257
                     /locus_tag="VHT22_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3136392.1"
                     /translation="MRSSGPEIVPFYAGEINRRAAQLSRAGKSIIPMHLGQPTRGPSD
                     ATIAAAQHALVAGPNGYWESHELKERIAEHYREWYSVEVAPERILLTNGASAALVAVF
                     ASMFATGERIALARPGYPAYRNTLTALGRVPVELDCGSASRYRLTASVLAQLEPAPHG
                     VVVASPANPTGTMLDDAELRALADECKRRDIVFISDEIYHGVTYRGRSVSALEVTQDV
                     IVINSFSKFCRMPGWRLGWLVVPPPLINKVQMYVGNFFLTAPNVAQAAAVAALGDGNE
                     LRGAIIEYARNREILLRELPKLGILPVVEPEGAFYIYADVSRYTQSSLEFCHKLLEET
                     GIALAPGVDFDPVGGGPFVRFFTAVSTQEIEQALERLHRWLQL"
     gene            2291..>2666
                     /locus_tag="VHT22_02205"
     CDS             2291..>2666
                     /locus_tag="VHT22_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX3136393.1"
                     /translation="MRQLLLVACLAFGADAAAVPTDSQIHRLIEERVQTIAGPEGGMG
                     IVVGVLDEKGPRVIAYGDTGAAERRPLNGDTVCEIASVTKVFSALLLADMVRTRDLKL
                     TDPVAKYLPKGTTLPEYNGRSIT"
BASE COUNT          462 a          860 c          882 g          462 t
ORIGIN      
        1 tcagcgcggt cgttgtggag tacgacgggc cgaatggcgt aagcttcgat cagaaaacga
       61 tactgtggct ggcggttctg ggcggcgcct tcgcagcgat ccttctggcg gttctctggt
      121 ggtggctcta cggacgctga agacaactcg ctacatatgg ccgatacaca acccaagctc
      181 gtcaaagtca cgaaccagcg cgagattccc atcaagccgg acatggtcct cggccgccag
      241 gcggagtgcg acctgcagtt gaccgaaggg catgcctcgc gcaagcacgc caagctgctc
      301 atcgccgacg acggtgtgtg gctcgaagat ctgaattcgg cgaacggcac cttcgtcaac
      361 ggcgccaaag tcaccggcaa agtgaagctg agctcgggcg accgcctgcg cttcgacgtc
      421 gaggagttcg atttccgcct tcccagcgct gcgccgccgg tcgacgatgg caagactcag
      481 tatcgcgagc ctgcgtcggc cgctgtcgac gctgcgagca gcggtctgta caagcggccc
      541 ggcgcctggg ccgatccgga cgcgatgggc gacggcgcga acaagaccaa gttcatcgac
      601 ccggcgcagc tcaagcagat ggtcaatgcg ccggcggcgg ccgccgtgaa cacggcgtcg
      661 atcgatggcc cgcacctgca tgtcgcgtcg ggcagtcgcg cgggattgaa catcaagctg
      721 acggtcggcg agtcgggtgt cagggaatgg actatcggca gccaggcgga tcgcgaagtg
      781 cagttccagg acagcggcgt gtccgcgctg cacgcgaaga tcgtcaatga aggcgagcgc
      841 tggaaagtgc tcgaccagat gtcggcgaac ggcacctacg tgaatggcaa gcgcagcaac
      901 gtcagctatc tcacggcggg cgaccgcgtg cggttcgggc cggtcgagtg catcttccag
      961 gcctcgcgcg ccggggcggc gccgcgccgt tccgccagcc cgtctgcgtc gtcggaggcg
     1021 gggagcgaat cgaagaacaa aaccctgctg atcgccggca tcgccttcgc cgtgacgatc
     1081 gtcgtgctgt ttctcgtcta caagtttgtc ttatagtcgg ggaatgcgct cttccggacc
     1141 cgaaatcgtt ccgttctacg cgggcgagat caaccgtcgt gccgcccagc tctcgcgtgc
     1201 cggcaaatca atcatcccga tgcatctggg ccagccgacg cgcggcccat cggacgcgac
     1261 gatcgctgct gcccagcacg cgctcgtcgc cggcccgaat ggttattggg aatcgcacga
     1321 gctcaaggaa cgcatcgccg agcactatcg cgagtggtac agcgtcgagg tcgcgcccga
     1381 gcgcatcctg ctgaccaacg gcgcgagcgc ggcactcgtc gccgttttcg catcgatgtt
     1441 cgcaacaggc gagcgcatcg cgctggcgcg gcccggctat ccggcctatc gcaatacgct
     1501 gaccgctctc gggcgcgttc cggtcgagct cgattgcggc tcggcgtcgc gctatcgcct
     1561 gactgcctcc gtgctcgctc agctcgagcc ggcgccgcac ggtgtcgtcg tcgcaagtcc
     1621 cgcgaatcca accggcacga tgctcgatga cgcggagttg cgggcgctcg cggacgagtg
     1681 caagcgtcgc gacatcgtct tcatctccga cgagatctat cacggtgtga cgtatcgcgg
     1741 acgctcggta tcggcgctgg aggtcacgca ggacgtgatc gtcatcaaca gcttctcgaa
     1801 gttctgtcgc atgccgggct ggcgtctcgg ctggctggtc gtgccgccgc cgttgatcaa
     1861 caaggtgcag atgtacgtcg gcaacttctt cctgaccgcg ccgaacgtcg cgcaggccgc
     1921 cgcggtcgcc gcgctcggcg acggcaacga gctgcgcggc gcgatcatcg aatacgcgcg
     1981 caaccgcgag atcctgctgc gcgagctgcc aaagctcggc atcctgccgg tggtggaacc
     2041 ggagggtgcg ttctacatct acgcagacgt gtcgcgctac acgcagtcga gcctggagtt
     2101 ctgccacaag ctgctggagg agacgggcat cgcgctcgcg cccggcgtgg atttcgatcc
     2161 ggtcggcggc ggaccgttcg tgcgcttctt tacggcggtt tcgacgcagg agatcgagca
     2221 ggcgctggaa cgcctgcatc gctggctgca gctgtaacct ttgacgcccg cgccgcgtcc
     2281 aacccggacc atgcggcaac tccttctcgt tgcatgtctc gcgttcggtg ccgatgcggc
     2341 tgcggtgccg actgacagcc agattcatcg actcatcgaa gagcgcgtcc agaccatcgc
     2401 cggaccggaa ggcggcatgg gcatcgtcgt cggtgtgctc gacgagaagg gaccgcgcgt
     2461 aatcgcttac ggcgataccg gcgctgccga gcggcgcccg ctgaatggcg acaccgtttg
     2521 cgagatcgcg tcggtgacca aggtgttcag cgcgctgctg ctggcagaca tggtccgcac
     2581 gcgcgacttg aagctgacgg atccggttgc gaagtacctg ccgaagggca cgacgctgcc
     2641 cgagtacaac ggccgctcga tcacgc
//
LOCUS       DATANS010000150         1866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_92339, whole genome shotgun sequence.
ACCESSION   DATANS010000150 DATANS010000000
VERSION     DATANS010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92339"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..831
                     /locus_tag="VHT22_02210"
     CDS             58..831
                     /locus_tag="VHT22_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3136394.1"
                     /translation="MRAPTLLLVCLVAAALAAPALAPYPPDQLDLAHRREAPSAAHRF
                     GTDDLGRDVLARVLYGARVSLAVGVLSAAVAGASGIAIGGIAGFAGGATDAVLMRATD
                     AMLAVPRLPLLMIAASVLQPTVTLLVVLIGMAGWMETARVVRAEFRTLRTRGFVEGAR
                     AAGAGRARLLVLHLLPNAAQAVVVSLTLAVARGILLESALSFFGVGVQPPQASWGNML
                     YQAQATLSTEPWLAVAPGLFILLTTVSVNLTGDRLARSR"
     gene            complement(897..>1866)
                     /locus_tag="VHT22_02215"
     CDS             complement(897..>1866)
                     /locus_tag="VHT22_02215"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX3136395.1"
                     /translation="PAATLLAIGERELQKAQADFEATASRVEPRRPGRSALEVWRNVL
                     DDHPKRGELPAAAQKTVDELLAFIRERRLVDLPEGERVVVAAAPAFDLGLASMHSSPP
                     LEPHPVKSFYYVTDAQADWPAGQQNAWLQKFNYPTLADISAHEVAPGHYVHSLFMRRT
                     PGKIRRIWIGLNPFPQPSSGQDGWAHYAEQLVTDEGFKREDPRYRLAQTSEALTRICR
                     LIAGLRLHSGDWTIDRAAEFFEREAHLPAPAARQEAVRGTYDPTYGGYFLGKMAAFKL
                     RRDYAAGRGAAFDLREFHERVMTNGIAPWWAHRQLLLPGDTRPVIE"
BASE COUNT          257 a          629 c          643 g          337 t
ORIGIN      
        1 caacgtcgtc gccgatgccg cacacgcact gattgacccg cgcgtcgtgg aggcgtaatg
       61 cgagcgccga cgctgctgct cgtgtgcctg gtcgcagcgg cgctggccgc gcccgcgctc
      121 gcaccctacc cgcccgatca actcgacctc gcccatcgcc gcgaagcgcc ttcggcggcc
      181 catcgctttg gcactgacga ccttggacgc gatgtgctcg ctcgcgtctt gtacggcgca
      241 cgggtgtcgc ttgcggtcgg tgtgctgtcg gcggctgtcg cgggcgcgtc gggcatcgcc
      301 atcggcggca tcgccggatt tgccggcggg gcgaccgacg cggtgctgat gcgcgcgacc
      361 gatgccatgc tggctgtgcc gcggctgccg ctgttgatga ttgcggccag cgtcctgcag
      421 ccgacggtga cgctcctggt cgtgctgatc ggcatggcgg gatggatgga gacggcgcgc
      481 gtcgtgcgcg ccgaattccg gacgcttcgc acgcgcggtt tcgtcgaagg cgcacgggcg
      541 gccggtgctg gccgggctcg gctgctggtc ctgcacctgc tgccgaacgc cgcgcaggcg
      601 gtggtggtgt cgttgacgct cgccgtcgcg cgcggaatcc tgctggagtc ggcgctcagc
      661 tttttcggcg tcggcgttca gccgccgcag gcgagctggg gcaacatgct ctaccaggcg
      721 caggcgacgt tatcgaccga gccatggctc gcggtggcac ccggcctctt cattctgctg
      781 acgacggtgt cggtgaacct gaccggtgat cggctcgctc gatcacgttg aacgcaaagc
      841 gcgcaaagat cgcaaagtca tgattgttgt ctgctgtctc ttgcaaactc tgcggtttat
      901 tcgatcaccg gacgcgtgtc gcccggcaat agcagctgcc gatgcgccca ccacggcgcg
      961 atgccgttgg tcatgacccg ctcgtggaat tcgcggaggt cgaacgcggc tccccgtccg
     1021 gccgcataat cgcgccgcag cttgaacgca gccatcttgc cgagaaaata gccgccgtag
     1081 gtcggatcgt aggtgccgcg aaccgcttcc tgacgcgcgg ccggggccgg caggtgcgcc
     1141 tcgcgctcga agaattccgc cgcgcgatcg atcgtccaat cgccgctgtg caggcgcaaa
     1201 ccggctatca gccggcagat ccgcgtcagc gcttcgctgg tctgggccag ccggtagcgc
     1261 ggatcctccc gcttgaagcc ttcgtccgtg acgagctgct ccgcgtagtg cgcccagccg
     1321 tcctgccccg acgatggctg cggaaacggg ttgagaccga tccagatgcg ccgaatcttg
     1381 ccgggcgtgc gccgcatgaa caggctgtgg acgtagtggc ctggcgccac ctcgtgggcc
     1441 gaaatgtcgg cgagcgtcgg gtagttgaac ttctgcagcc acgcgttctg ctgcccggcg
     1501 ggccagtcgg cctgcgcatc ggtcacgtag tagaagctct taaccggatg cggctcgagc
     1561 ggcggcgacg agtgcatcga cgccaggccc agatcgaacg ccggagcggc cgcgacgacg
     1621 acgcgctctc cttccggcag gtcgacgagg cgccgctcgc ggatgaaggc cagcagttcg
     1681 tccaccgtct tctgggctgc cgctggcagt tctccccgtt tcggatggtc gtcgaggacg
     1741 ttgcgccaga cttccagcgc tgatcgtccc ggccggcgtg gttcgacacg cgaggcggtc
     1801 gcttcgaaat cggcctgcgc tttctgcagc tcgcgctcgc caatcgccag caacgtcgcc
     1861 gcgggg
//
LOCUS       DATANS010000151         1588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_302064, whole genome shotgun sequence.
ACCESSION   DATANS010000151 DATANS010000000
VERSION     DATANS010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302064"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VHT22_02220"
     CDS             complement(<1..636)
                     /locus_tag="VHT22_02220"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3136396.1"
                     /translation="MPAAATQHTPAFRPLYLQLRDLLLRDMETRQWRPGEALPSEIEL
                     AARFGVSQGTVRKAIDALAADNLVVRRQGKGTFVATHTEEKTSMFRFLRIRRSDDRDE
                     YPASRLLDVRRGKASAEVARLLEIKPSEAVFVVRRVLEYGGEPAVLDDIMLPATLFKG
                     LTRARLEAYRGSMYGFFETQFGVRMLRAQERLKAIAADSATAAILKVRTGEP"
     gene            850..>1588
                     /locus_tag="VHT22_02225"
     CDS             850..>1588
                     /locus_tag="VHT22_02225"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002061257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX3136397.1"
                     /translation="MKKPVRIAVTGAAGQIGYSLLFRIANGDMLGRDQPVLLQLLELP
                     IDKAQAALKGVMLELEDCAFPLLAGMSGSGDPKAAFKDAEMALLVGARPRGPGMERKD
                     LLLENARIFIEQGKAMDAVASRDIKVLVVGNPANTNAYIAMKSARGLPQKNFTAMLRL
                     DHNRALSQLAAKSGKPVGSIDKLIVWGNHSPTMYPDYRFATADGESLKALINDENWNR
                     TVFLPTVGKRGAAVIEARGLSSAASAAN"
BASE COUNT          297 a          523 c          497 g          271 t
ORIGIN      
        1 cggctcacca gtgcgaacct tcaggattgc cgcagtggcg ctgtcggcgg cgatcgcctt
       61 cagtctttcc tgcgcccgca acatgcgtac gccgaactgc gtttcgaaga atccgtacat
      121 cgagccgcga taagcttcga gccgggcccg cgtcagcccc ttgaacagcg tcgcaggcag
      181 catgatgtcg tcgagcacgg ccggctcgcc gccgtactcg agaacccggc gcacgacgaa
      241 gaccgcttcc gagggcttga tctcgagtaa tcgcgccacc tcggccgacg ccttcccgcg
      301 gcgcacatcg agcagccggc ttgccgggta ttcatcgcgg tcgtcgctgc gccggatgcg
      361 cagaaaccgg aacatcgacg tcttttcctc ggtatgcgtg gcgacgaagg tccctttgcc
      421 ttgccgccga acgaccagat tgtcggcggc cagcgcatcg atcgccttgc gcaccgtgcc
      481 ttgcgaaacg ccgaaccgcg cggccagctc gatctcgctc ggcagcgcct cgccgggacg
      541 ccactggcgc gtttccatgt cgcgcagcaa cagatccctg agctgcagat acagcggccg
      601 gaaggccgga gtgtgttggg tggcggcagc gggcatcgac gatacgggtg cacaaactgc
      661 cgcccggcaa aaaagaaggc ggcgacacct cttttatatc ttatatctta tataagagtc
      721 aattgacacc cggcagccaa agcccgtaga attttcctgc aggggcgcgc catgccgacg
      781 gcggcttttc cgcccgtggc ctgccgggat gcctgaagtc tcccgaacca acccgtcctg
      841 gagctctcga tgaaaaagcc cgtccgcatc gcggtgaccg gcgccgccgg ccagatcggc
      901 tatagcctcc tgttccggat cgccaacggc gatatgctcg gacgtgacca gccggtgctg
      961 ctgcaattgc tcgagctgcc gatcgacaag gcgcaggccg cgctcaaggg cgtaatgctc
     1021 gagctcgagg attgcgcctt tccgctgctc gccggcatgt ccggcagcgg cgaccccaag
     1081 gcggctttca aggatgcgga gatggcgctg ctggtcggcg cacggcctcg cggccccggc
     1141 atggagcgca aggatctgct tctggagaac gcgaggattt tcatcgagca gggcaaggcg
     1201 atggacgcag tcgccagccg cgacatcaag gtgctggtcg tcggcaatcc ggccaatacc
     1261 aacgcctaca tcgcgatgaa gtccgctcgc ggactgccgc agaaaaactt caccgccatg
     1321 ttgcggctcg atcacaatcg cgcgctgtcg cagcttgcgg ccaagtcggg caagccggtg
     1381 gggtcgatcg acaagctgat cgtctggggc aatcactcgc caacgatgta tcccgattat
     1441 cggttcgcga cagccgacgg agaatcgctg aaggcgctga tcaacgacga gaactggaac
     1501 cgcacggtgt tcctgccgac cgtcggcaaa cgaggcgccg cggtaatcga agcccgtgga
     1561 ctgtcgtcgg ccgcgtcggc cgccaacg
//
LOCUS       DATANS010000152         1627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_7386, whole genome shotgun sequence.
ACCESSION   DATANS010000152 DATANS010000000
VERSION     DATANS010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7386"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1126)
                     /locus_tag="VHT22_02230"
     CDS             complement(32..1126)
                     /locus_tag="VHT22_02230"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEX3136398.1"
                     /translation="MPSELYIGLMSGTSVDGVDAVLADFAAAPWRVRARRHVAFGPAL
                     RAELDALQKSGADELHRSALAANALMDCCAAAVSGVLADARLESSDIAAIGLHGQTVR
                     HRPELQFTTQLANPARLAEACRIPVVADFRSRDVAAGGQGAPLAPALHAALFRDAARH
                     RVVLNIGGIANITDLPPDGPVRGFDTGPGNTLLDAWCMRHRGEAFDRDGAWAATGRPI
                     AALLEALAADPYFTLPPPKSTGRDHFNLRWLESRLQSSYAPADVQRTLLALTSATIAE
                     AIAAHCAGASEVVVCGGGAHNGALLRELARDLGGRALTTTAAYGVPVGDVEALAFAWL
                     ARESLGGRPGNLPAVTGARGPRVLGAVYPA"
     gene            complement(1141..>1627)
                     /locus_tag="VHT22_02235"
     CDS             complement(1141..>1627)
                     /locus_tag="VHT22_02235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX3136399.1"
                     /translation="EFSRITSGFSNARFHPILQTWRAHKGVDYAAPTGTPVRAVGDGK
                     VIFAGRQNGYGNVVILQHGGANSTVYAHLSRFAAGTKPGARVGQGTVIGYVGQTGWAT
                     GPHLHYEFRVNSVQRNPMTIALPDAPPLAAADRPAYVARIAPLADALALGQVVTIAAG
                     E"
BASE COUNT          264 a          577 c          565 g          221 t
ORIGIN      
        1 ccgtttttca ttccgcgttc gacagccggc gctacgccgg atagacggcg cccagcacac
       61 gcgggccccg cgcgccggtc accgccggca gattgcccgg cctcccgcca agcgattcgc
      121 gagcgagcca cgcgaacgcc agcgcctcga catcgccaac cggaacgcca tacgccgcgg
      181 tcgtcgtgag cgctcgaccg ccgagatcgc gggcgagctc gcgcaacagc gcgccgttgt
      241 gcgcaccgcc gccgcatacc acgacctcgg acgcgcccgc gcaatgcgcc gctatcgctt
      301 cggcgatcgt cgcggaggta agcgccagca aggttcgttg cacgtcggcc ggcgcgtatg
      361 acgattgcag ccgcgactcc aaccagcgca ggttgaagtg atcgcgcccg gtgctcttcg
      421 gcggtggcag cgtgaaatac ggatccgccg cgagcgcctc gagcaatgcc gcgatcggcc
      481 ggccggtcgc cgcccatgcg ccgtcgcggt cgaacgcctc gccgcggtgc cgcatgcacc
      541 acgcatcgag cagggtgttg cccggtccgg tgtcgaagcc tcgtaccggc ccatccggcg
      601 gcaggtcggt gatgttcgcg atgccgccga tattgagcac gacgcggtgc cgcgcagcgt
      661 cccggaacag cgcggcgtgc agcgcgggcg cgagcggcgc gccctgcccg cccgcggcga
      721 catcgcggct gcggaaatcg gcgacgacgg ggatgcggca ggcttcggcg agtcgcgccg
      781 gattggcaag ctgggtcgtg aactggagct ccggccgatg acgtaccgtc tgtccgtgca
      841 ggccaatcgc ggcgatgtcc gaagattcaa gacgcgcgtc ggcaagaacc cccgacacag
      901 cagccgcgca gcaatccatc agcgcattgg cggcgagcgc cgatcggtgc aattcatcgg
      961 cgccgctttt ttgcagagca tcgagctcgg cccggagggc gggaccgaac gcaacgtggc
     1021 ggcgcgcgcg gactcgccag ggcgcggcgg cgaaatcggc cagcaccgcg tcgacgccat
     1081 cgacgctggt gcccgacatc aggccgatgt agagctcgct gggcaaccgc tgcttgacag
     1141 ctactcgccg gcagcgatgg tgacgacctg gccgagcgcg agcgcatcgg caagcggcgc
     1201 gatgcgcgcg acatacgccg gcctgtccgc ggcggccagc ggcggcgcat ccggcagcgc
     1261 gatcgtcatc gggttgcgct gcacgctatt gacgcggaac tcgtagtgca gatgcgggcc
     1321 ggtcgcccag ccggtctgac cgacgtagcc gatcaccgtg ccctggccga cgcgcgcgcc
     1381 gggcttcgtc ccagcggcaa accgcgacaa atgcgcatac acggtcgagt tggctccccc
     1441 atgctgcagg atgacgacgt tgccgtaacc gttctgccgg ccggcgaaga tgaccttgcc
     1501 gtcgccgacc gcgcggaccg gagtgccggt gggcgccgcg taatcgacgc ccttgtgcgc
     1561 gcgccacgtc tgcagaatcg gatgaaagcg cgcattcgaa aagcccgagg tgatccgcga
     1621 gaactcc
//
LOCUS       DATANS010000153         3100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_679625, whole genome shotgun sequence.
ACCESSION   DATANS010000153 DATANS010000000
VERSION     DATANS010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679625"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VHT22_02240"
     CDS             <1..758
                     /locus_tag="VHT22_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX3136400.1"
                     /translation="AVVIAAAKSDRSDLVWAGPLPAGADKWSSRAQPPRPPLGPRQGE
                     IRPFFLASGADYRAPPPPGYDSPAFRANLAEVRAISDRRSIEQVRIAQYWEGLTGSFN
                     AGYWNDVTKRAIVAHRLGEADSARVLALVHMVAVDATIACHDSKYVYWVPRPTQLDPG
                     IHLAIAVPNHPSYPSNHACISGAIGLVLDAQFPDEGGRYFAMAREAGESRIYGGIHYR
                     FDIDEGFVIARKVAARALATGVPVDRPFVPIGG"
     gene            complement(776..1603)
                     /locus_tag="VHT22_02245"
     CDS             complement(776..1603)
                     /locus_tag="VHT22_02245"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3136401.1"
                     /translation="MARNPTATLGERLTRVGLIVLTGAVLLFLVAPILTIVPLSFSSG
                     SFFYYPLPGLSLRWYQDFFTSSFWLSSLQNSLIIGISATVLATVLGTMAALGIWRARF
                     PAQALVLAMLISPMVVPVVIIAVGVYFAFAPLGLTDGYLGLILAHATLGVPFVVITVL
                     ATLSAFDRTLLRAAESLGASQLTTFRRVMLPLILPGVASGAVFAFAASFDEVVVVLLM
                     AGPAQRTLPRQMFAGINDNISLTIAAAATMLIAISLTLMIAVGALQRRSARMRQAVG"
     gene            complement(1608..2876)
                     /locus_tag="VHT22_02250"
     CDS             complement(1608..2876)
                     /locus_tag="VHT22_02250"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3136402.1"
                     /translation="MLRGAAGAVHAALASVDRDPADLRRALRRSRRREQWRAAALVLP
                     LFVFLAACFVVPIAAMLARGIIDTDVARILPAVTSALKSWDGGGLPSESAYAALVADI
                     RAARAAGTLASAATRLNYDEPGFRSLLFTTGRQLPDELSAPAKETLIGIDPKWGERET
                     WAAIRRAGGPVTDFYLLAALDLQRDADGSITGGPVEQRVFRDVLGRTLWISAMVTLIC
                     LVLGYPVAYVIAAQPPGRAGMLLFLVLLPFWTSLLVRTVAWVVLLQKEGVLNSLFMSL
                     GLVHEPLKMIYNRFAVYVAMVHVLLPFMILPLYSVMRGIPPSYVRAASSLGARPFTAF
                     RRVFVPQTLAGIGAGCLMVFIQALGYYITPALVGGAEDQMISYFIAFYASKTVNWGMA
                     AALSIMLLTATLLLYAVYNRMVGVERMRLG"
     gene            complement(2910..>3100)
                     /locus_tag="VHT22_02255"
     CDS             complement(2910..>3100)
                     /locus_tag="VHT22_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3136403.1"
                     /translation="NLPPKIPYGPTTKASTALIDKNVLPELATAPQNMASALPINDKF
                     WLENLDKLSQRFNAWVAK"
BASE COUNT          581 a         1030 c         1034 g          455 t
ORIGIN      
        1 cggccgtcgt cattgcggct gccaagtccg atcggtccga tctggtgtgg gccggcccgc
       61 tgccggccgg cgcagacaaa tggtcgagcc gggcacaacc gccgcgcccg ccgcttgggc
      121 ctcggcaggg cgagattcgc ccattctttc tcgccagcgg cgccgactat cgggccccgc
      181 cgccaccggg gtacgactcg ccggcttttc gggcaaatct agcggaggtc cgcgcgattt
      241 ccgatcggcg cagcatcgag caagtgcgca tcgcgcaata ttgggagggc ctgacgggct
      301 cgttcaacgc gggctactgg aacgacgtga caaaaagggc gatcgtcgcg cacaggcttg
      361 gcgaggccga ctcggcgcgc gtgctcgcgc tggtccatat ggtcgccgtc gatgcgacca
      421 tcgcctgcca cgattccaaa tacgtgtact gggtcccccg gccaacgcag ctcgacccgg
      481 gcattcacct ggcgatcgcc gtcccgaatc acccgtcgta cccgagcaat catgcatgca
      541 tctcgggtgc catcggcctg gttctggacg cgcaattccc ggacgagggc ggccgatatt
      601 tcgcgatggc gcgcgaagcc ggcgaatccc gcatctatgg cggcatccat taccgcttcg
      661 acatcgacga gggattcgtg atcgcgcgca aggtcgcggc gcgcgcgctg gcgaccggag
      721 ttcccgtcga ccgaccgttc gtcccgatcg ggggctaggt ccgaagcgaa cgcggctatc
      781 ccactgcctg ccgcatccgc gcgcttcgcc gctgcaaagc gccgacggcg atcatcagcg
      841 tcagtgagat cgctatcagc atcgtcgccg ccgcggctat ggtgagactt atgttgtcgt
      901 tgatgccggc gaacatctga cgcggcagcg tgcgctgcgc gggcccggcc atcagcaaga
      961 cgacgacgac ctcgtcgaac gatgccgcga acgcgaacac cgcgcccgac gccacgcccg
     1021 gcaggatcaa tggcagcatg acgcggcgaa acgtcgtgag ctgcgaagcg ccgaggcttt
     1081 ccgccgcgcg gagcagcgtg cggtcgaacg cggaaagcgt ggccagcacg gtgatgacga
     1141 cgaacggcac accgagcgtt gcatgcgcga ggatgaggcc gaggtagcca tcggtgaggc
     1201 cgagcggcgc gaaggcgaag tagacgccga cggcgataat gaccaccggt acgaccatcg
     1261 gcgagatcag catcgccagc accagcgcct gagccggaaa gcgtgcgcgc cagatgccga
     1321 gcgccgccat cgtcccgaga acggtggcca gcaccgtcgc cgatatgccg atgatgaggc
     1381 tgttctgcag cgatgacagc cagaacgacg aagtgaagaa gtcctggtac cagcgcaggc
     1441 tcaatcccgg cagcggatag tagaagaaag atcccgagga aaacgacagc ggcacgatgg
     1501 tcaggatagg cgcgaccagg aacagcagca ctgcgccggt cagcacgatc aggccgacgc
     1561 gggtgagccg ctcgccaagc gttgcggtcg ggtttcgcgc cattgcgtca gcccagccgc
     1621 atccgctcga cgccgaccat gcggttgtac accgcgtaga gcagcagtgt cgcggtaagc
     1681 agcatgatcg acagcgccgc agccatgccc cagttgacgg tcttgctggc gtagaaggcg
     1741 atgaagtagc tgatcatctg gtcttcggcg ccgccgacca gcgccggcgt gatgtagtag
     1801 cccagcgcct ggatgaaaac catcaggcag ccggcgccga tgcccgccag cgtctgcggg
     1861 acgaacacgc gccggaacgc ggtgaacggc ctggcgccga gcgaggatgc ggcacgcacg
     1921 tacgaaggcg gaatgccgcg catcacgctg tacagcggca ggatcatgaa cggcaagagc
     1981 acgtgcacca tcgccacgta gacggcgaag cggttgtaga tcatcttcag cggctcgtgc
     2041 accagcccga gcgacatgaa caggctgttc agcacgcctt ccttctgcag caacacgacc
     2101 caggcgacgg tgcgcaccaa gagcgaggtc cagaacggaa gcagcaccag gaacagcagc
     2161 atgcccgcgc gtcccggcgg ttgcgcggcg atcacataag cgaccgggta gccgagtacc
     2221 aggcagatca gcgtcaccat cgccgagatc cacagcgtgc gtccgagaac gtcgcgaaac
     2281 acgcgctgct ctaccgggcc accggtgatc gaaccgtcgg cgtcgcgctg caggtcgagc
     2341 gccgcgagca ggtagaagtc ggtgaccgga cctccggcgc ggcgtatcgc ggcccaggtt
     2401 tcgcgctcgc cccacttcgg atcgatgccg atcagcgttt ctttcgccgg cgccgacagc
     2461 tcgtcgggca actgcctgcc ggtggtgaac agcaagctgc gaaagcccgg ctcatcgtaa
     2521 ttcaggcgcg tggcggcgct cgccagggtg cctgcggcgc gcgccgcgcg aatgtcggcc
     2581 acaagcgccg cgtacgccga ctccgacgga agcccgccgc cgtcccagct tttgagcgcg
     2641 ctcgttaccg ccggcagaat gcgcgccaca tcggtatcga tgatgcctcg cgcgagcatc
     2701 gcagcgatcg gcacgacgaa gcacgccgcg agaaagacga acagtggcag cacgagtgcc
     2761 gccgcgcgcc attgctcgcg acggcggctt cggcgcagcg cccggcgcaa atccgccgga
     2821 tcgcggtcga cgctcgccag cgcggcgtgg acggcacccg ccgcgccgcg cagcatcggc
     2881 ttctcgcctt tagatgcgga tggaaccgac tacttggcga cccaggcgtt gaaccgctgt
     2941 gacagcttgt ccaggttctc cagccagaac ttgtcgttga tcggcagcgc agacgccatg
     3001 ttctgcggcg cggtggcgag ctccggcagc acgttcttgt cgatcagcgc ggtcgatgcc
     3061 ttggtcgtcg ggccgtacgg aatcttcggc ggcaggttct
//
LOCUS       DATANS010000154         1984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_555290, whole genome shotgun sequence.
ACCESSION   DATANS010000154 DATANS010000000
VERSION     DATANS010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555290"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(432..1799)
                     /locus_tag="VHT22_02260"
     CDS             complement(432..1799)
                     /locus_tag="VHT22_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX3136404.1"
                     /translation="MTDVTTLPFDLPSVRRKKLTVDFDGGNQSSNGGLLLLRQAERKL
                     GICGRLADAMPDRRDASRIRQEMFEMVMARVSAIACGHEDAIDLDRLRHDPLLKVAVG
                     RCPVSGAPLASQSTISRLENAPSKTEAARLTAALIDQAGTTVKPRKQEILDIDDTFCA
                     AHGGQQLAFWNAHHDERGFASMHIYHVTSGTPVVAILRPARTPKGTEVRTVIKHVTKR
                     LRRHWRRTRIVWRGDSHYGRLEAMEWAENNDADYIFGLAGNAALDALVAETAVNLRFH
                     HAMSSKPKLRTFASFTYQASSWKRPRKVVARLECSLQPTAGEVGMRQEVDIRYVVTSL
                     PGSAQHLYEKVYCQRGQMENLIKLHKAQLASDRMSCHSATANQVRLVLHTAAFWLMHG
                     VRAAIPRTDPLAGSEFATIRERLIKIGARVIEHIARIRIQLPTSCPERALFRAVALGL
                     LPSGP"
BASE COUNT          337 a          641 c          638 g          368 t
ORIGIN      
        1 ttgcacgccg aaacacaaaa catccagcca agccagatca agtcgtgtgc cagcgcgtcg
       61 ccatcctccg gcgcgagcgc agcaacgtgc ccgcccggca aaagtcgcgc cgaccgcgcg
      121 cgacgctatt ttgggtgaat aagaacggct agtagttgtt gacgatagcg gacgccgact
      181 gattttttgg cagctctgca ggcttggccg ccgcagactt aggctctctc aaattgggcg
      241 cggcgcccgc cgccactgag cgccctagcc gggatcatgc accacaaggc gggttttgcg
      301 cgactgtttg agcgggcgcg cgctggcgat ccgaccggct gggtcggtgt gagatgactc
      361 gctgtgcgac gcgtgtgcgg ttgatttgcc tagtcggcgg ctcgtcgggg cgctcatatc
      421 agccccgcag ttcatgggcc ggacggcaac aagccgaggg cgacggcgcg gaacaatgcc
      481 cgctccgggc aactggtcgg caactggatg cggatgcgcg cgatgtgctc gatcacacgc
      541 gcgccgatct tgatcaggcg ctcgcggatg gtcgcgaact cgctgccggc gagcggatcg
      601 gtccgcggga tggcggcgcg cacgccatgc atcagccaga acgcggcggt gtgaagaacc
      661 agccgcacct gattggcggt tgcgctgtga cacgacatgc gatccgacgc caactgcgct
      721 ttgtgcagct tgatcagatt ctccatctgg ccgcgctggc aatagacctt ctcgtaaagg
      781 tgctgcgccg agcccgggag cgaggtgacg acgtagcgga tgtcgacctc ctggcgcatg
      841 ccgacctctc cggccgtcgg ctgcaatgag cactcgagcc gagccaccac cttgcgtggc
      901 cgtttccagc tgctggcctg gtaagtgaag ctggcaaagg tgcgcagctt cggcttgctg
      961 ctcatggcat gatggaagcg cagattgacc gcggtctcgg ccaccagggc gtcgagcgcg
     1021 gcattgccgg caaggccaaa aatgtagtcc gcgtcgttgt tttccgccca ttccatggcc
     1081 tcgaggcggc cgtagtggct gtcgccgcgc cagacaatgc gggtcctgcg ccaatgtctg
     1141 cgcagccgct tcgtcacatg cttgatgacg gtcctcacct cggtgccctt cggggtgcgc
     1201 gcagggcgca ggatcgccac caccggcgta ccgctcgtca cgtgatagat gtgcatcgac
     1261 gcgaagccgc gctcgtcgtg atgcgcgttc cagaacgcaa gctgctgacc gccgtgcgcc
     1321 gcgcagaacg tgtcgtcgat gtcgaggatc tcctgcttgc gcggcttcac tgtcgtgccg
     1381 gcctggtcga tcagagcagc ggtgagccgc gccgcctctg tcttgctcgg cgcgttctcc
     1441 agacggctaa tcgtcgattg cgaggccagc ggcgcaccgc tgaccggaca gcgcccgacc
     1501 gcgaccttca acagcgggtc atgccgcagc cggtcgaggt cgatggcatc ctcatggccg
     1561 cacgcgatcg ccgagacgcg cgccatcacc atctcgaaca tctcctgacg gatgcggctc
     1621 gcatcgcggc gatccggcat cgcatcggca agccgcccgc aaatgccgag cttgcgctcc
     1681 gcctgtcgca gcaacagtag gccgccattc gacgattggt tgccgccgtc aaaatccact
     1741 gtgagtttct tgcgtcggac agatggcagg tcgaagggga gcgttgtaac gtcggtcatg
     1801 gcggctgtgg cgctcggctc gatccgcgag gcaattcgtc tgaacaccga tttgctacgt
     1861 gaatcgctcg cttcgcgcta cagccgtcac ttaaatctca ccctcgtgca taatccgggc
     1921 tagacaccca ttgtcctttg acaccgtcgg catgagtccg tgcggtccgc gaaacacccg
     1981 gttg
//
LOCUS       DATANS010000155         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_679799, whole genome shotgun sequence.
ACCESSION   DATANS010000155 DATANS010000000
VERSION     DATANS010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679799"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1470)
                     /gene="pcnB"
                     /locus_tag="VHT22_02265"
     CDS             complement(112..1470)
                     /gene="pcnB"
                     /locus_tag="VHT22_02265"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEX3136405.1"
                     /translation="MIRRFLGKVFSGKLFKSAQPKVIPFKTHRIASDHVSRCALKTCE
                     TLQQRGHAAYVVGGAVRDLLLGGIPKDYDVATGATPEEVRAIFRRSRIIGRRFRLVHV
                     MCGAETIEVSTFRGVAGVEATDAGDHSTDEHGRILRDNVFGSMEDDARRRDFTVNAMF
                     YDPVRGEVIDYHDGVADIKAKKLRMIGDPAQRYREDPIRMLRAVRLAAARGLEIETRT
                     RKPIRELAGLLLNVPKARVFDEMMNLLLSGHVADGVRRLRKEGLHHGVLPLLDVIFEQ
                     PLGERFVMLALENTDKRINEGKTVSPGFLFASLLWHEVLAAWKAAQARGMHTMPALFH
                     AMDQVLQVQAEKLAIPRRYGADMKDIWALQARFLNRAGRRPWQLLEHPRFRAGFDFLL
                     LRCASREIDPELGAWWEKFQHADEATRAGMLVKDTPGTSKKRRRPRRKKKPAAAATES
                     AG"
BASE COUNT          258 a          519 c          500 g          279 t
ORIGIN      
        1 ccaggaattg cgtgtgcggc agcgtcgcga gtgcggcgag gcccgcccgc acctgcacga
       61 cggggtcttc gagattggcc ccgagcgcga cgtaggcggt gaccggcgcc atcagcctgc
      121 ggattcggtg gccgccgcag cgggcttttt cttgcgccgg ggacggcggc gtttcttgct
      181 cgtgccgggc gtgtctttga ccagcatgcc ggcgcgcgtc gcctcgtccg cgtgctggaa
      241 cttttcccac cacgcaccga gctcgggatc gatctcgcgg ctcgcgcagc gcagcagcag
      301 gaaatcgaag ccggcgcgaa accgcggatg ctcgagcagc tgccacggcc ggcgcccggc
      361 gcggttcaga aagcgcgcct gcaacgccca gatgtccttc atatcggcgc catagcggcg
      421 cggtatcgcg agcttctccg cctgcacctg cagcacctgg tccatcgcgt gaaacagcgc
      481 gggcatcgtg tgcatgccgc gcgcctgcgc cgccttccac gccgcgagca cttcgtgcca
      541 cagcagcgat gcgaacagga agccgggcga caccgttttg ccttcgttaa tgcgcttatc
      601 cgtattttcg agcgcgagca tcacgaatcg ctcgccgagc ggctgctcaa atatcacgtc
      661 gagcagtggc agcacgccat gatgcaggcc ttccttgcgc agccgccgga cgccgtcggc
      721 gacgtgcccc gacagtagca agttcatcat ttcgtcgaac acgcgcgctt tgggcacgtt
      781 gagcaacagg cctgcgagct cgcggatggg cttgcgcgtg cgcgtttcga tttcgaggcc
      841 gcgcgccgcc gcgagccgca ctgcgcgcag catgcggatg ggatcttcgc gatagcgctg
      901 cgcgggatcg ccgatcatgc gcagcttctt cgccttgatg tcggcgacgc cgtcgtgata
      961 atcgatgacc tcgccgcgca ccggatcgta aaacatcgcg ttgacggtaa agtcgcggcg
     1021 gcgcgcgtcg tcttccatgc tgccgaacac gttgtcgcgc aggatgcgcc cgtgctcgtc
     1081 ggtcgaatgg tctcctgcgt cggtggcttc gacgcccgcc acgccgcgaa acgtggagac
     1141 ctctatcgtc tccgcgccgc acatcacgtg cacgagccgg aagcgccggc cgatgatgcg
     1201 cgagcggcgg aagattgcgc gcacttcctc aggcgtggca ccggtggcga cgtcatagtc
     1261 cttgggaatg ccgccgagca gcaggtcgcg caccgcgccg ccgacgacat acgcggcgtg
     1321 cccgcgctgc tgcagcgtct cgcaggtctt cagtgcgcag cggcttacgt gatcgctcgc
     1381 gatgcgatgc gtcttgaacg gaatgacctt gggctgcgcg gacttgaaca gcttgcccga
     1441 aaacaccttg ccgagaaaac gccggatcat taaatccctg gccctttaag tcgaaatctc
     1501 atcgcctaaa aaacaaattg agcggcgcac tgccaagtgc gtccgctcga atttgt
//
LOCUS       DATANS010000156         2670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_763880, whole genome shotgun sequence.
ACCESSION   DATANS010000156 DATANS010000000
VERSION     DATANS010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763880"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VHT22_02270"
     CDS             complement(<1..526)
                     /locus_tag="VHT22_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3136406.1"
                     /translation="MARRTRDRGQPKVLVIDDEPDLLELLELTLSRMGLDTTRAQSVS
                     EAIQLLDSQAFDLCLTDMRLPDGEGLAVVEHITEKALDVPVAVITAFGSAENAVAALK
                     AGAFDYLAKPVALEQLRALVKQALKVPEKPSAPSSYQLLGESAAIMQVRSMIERLSKS
                     QAPVFITGESGSGKE"
     gene            complement(551..2182)
                     /locus_tag="VHT22_02275"
     CDS             complement(551..2182)
                     /locus_tag="VHT22_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3136407.1"
                     /translation="MASDSSIPSLEPTSLPTLINESGRRILWIVGVYRAVCAALLLST
                     ALFLDLRSAGIVAPNAFVTAAGLYFLFSMAAFWWVQRDSFDHPLAPLLLSLLLGDIFF
                     IALLTYASGGAGTPLSILLFPQLAASGWLMRTQIAFFHAALASSVLIALEGWRLIEGD
                     VGAAQILQMGLVGFGYFATVAIALALGTFTKASEDLAVQRGIDVANLEQVNRLIIQDM
                     QDGVLVVDLNGVVRGHNAQVTRLLGGFGRMRGGMRLAEFSATLHDYWRRWQEDFSEAL
                     PPFKVDATQRLLRVRHVRIGSGLNGGTLIYLEDLGRAQSDAQQMKLAAMGRLTASIAH
                     EVRNPLSAINQAAQLLEEDGSVAPEGERLLSMIRNNAKRIDRIVGEVLQLNRRDRQQP
                     EVIDYSEFIYSLVEEVVQAEDIPPASVIIQCPSELYVLFDRGHLSQIMWNLVRNAWQH
                     CQKKDGSIHIMGRAGYMGDAVISELADDGPGIPAELRAQIFEPFFTTRPGGTGLGLYI
                     ARELADANGAALELLPKGPGANFRLTLKRAANPPT"
     gene            complement(2188..2418)
                     /locus_tag="VHT22_02280"
     CDS             complement(2188..2418)
                     /locus_tag="VHT22_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HEX3136408.1"
                     /translation="MGKIIAWLVLIFIVLLALRLVNQRKSRKQQRAAGAAGSTAVEPM
                     VRCARCGVFLPRADAIQRAGEYACPDGQCVKR"
     gene            complement(2440..>2670)
                     /locus_tag="VHT22_02285"
     CDS             complement(2440..>2670)
                     /locus_tag="VHT22_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX3136409.1"
                     /translation="PPSIDAAVFIEHTARATAIVYDLVAEFGGSFSAEHGIGQMKRDA
                     MARYKAPIELELMRRIKRALDPHATLNPGKVV"
BASE COUNT          482 a          881 c          850 g          457 t
ORIGIN      
        1 gctccttgcc gctgcccgat tcgccagtga tgaataccgg cgcctggctt ttcgacagcc
       61 gctcgatcat cgaccgcacc tgcatgatcg ccgccgattc acccagcagc tgatacgacg
      121 aaggcgcgga aggcttctcc ggaaccttga gcgcctgttt gaccagcgcg cgcaactgct
      181 cgagcgcgac cggcttcgcc agataatcga atgcaccggc cttgagcgcg gccacggcgt
      241 tctctgcgct gccgaaggcg gtgatgaccg caaccggcac gtcgagcgcc ttttcggtga
      301 tgtgctcgac gacagcgagt ccctcgccgt cgggaagtcg catgtcggtc agacagaggt
      361 cgaaagcctg gctgtcgagc agctggatcg cttcgctgac ggactgtgcg cgcgtcgtgt
      421 cgagacccat ccgagacaag gtgagctcga gcagctcgag caggtccggt tcatcgtcga
      481 tgaccagcac tttcggttgc ccgcgatcgc gtgttcttcg cgccatagtg cctcccaagg
      541 caatagccgg ctaggtcggc gggttcgccg cccgcttcaa cgtcaatcta aaattggcac
      601 ccggaccctt gggcaagagc tcgagcgccg cgccattcgc atcagcaagc tcccgggcaa
      661 tgtacaagcc cagcccggtc ccgcccggcc gcgtcgtgaa gaacggctcg aatatctgcg
      721 cccgcaactc ggccggaatg cccgggccgt cgtcggcaag ctcggatatt acggcatcgc
      781 ccatatatcc ggcgcggccc atgatgtgga tgctgccgtc cttcttctgg cagtgctgcc
      841 atgcgttgcg caccaggttc cacatgatct ggctcaagtg gccgcgatcg aacagcacgt
      901 acagctcgct cggacactgg atgatcacgc tcgcgggcgg gatgtcctca gcctgcacga
      961 cttcctcgac cagcgagtag atgaattccg aataatcgat gacctccggt tgctggcggt
     1021 cgcggcgatt gagctgcagc acttcgccga cgatgcgatc gatgcgcttg gcgttgttgc
     1081 ggatcatcga caggagacgc tcgccttcgg gcgcgaccga gccatcctcc tcgagcagct
     1141 gcgcagcctg gttgatcgcc gacagcgggt tgcgcacctc gtgcgcgatg ctcgcggtca
     1201 gccggcccat cgccgccagc ttcatctgct gcgcatccga ctgggcgcgt cccaggtcct
     1261 cgaggtagat aagcgtgccg ccgttgagcc ccgatccaat gcgcacgtgc cgcacgcgca
     1321 gcagccgttg cgtcgcgtcg accttgaacg ggggcagggc ttcggagaaa tcctcctgcc
     1381 agcggcgcca gtagtcgtgc aaggtcgcgc tgaattcggc gagccgcatg ccgccgcgca
     1441 tgcgaccgaa tccccccagc agccgggtca cttgtgcatt gtggccgcga acgacgccgt
     1501 tcagatcgac gaccagcacg ccatcctgca tgtcctgaat gatcagccgg ttcacctgct
     1561 cgaggttggc gacgtcgatg ccccgctgta cggcgaggtc ttcgctcgcc ttggtgaacg
     1621 tcccgagagc gagtgcgatt gcgaccgtcg cgaaatagcc gaagccgacc aggcccatct
     1681 gcaggatctg tgccgcgccg acgtcgcctt cgatcaaccg ccagccttcg agcgcgatca
     1741 gcaccgagct tgccagcgcc gcatgaaaga aggcgatctg cgtgcgcatc agccagccgc
     1801 ttgcggcaag ctgcggaaac agcagtatcg agagcggggt tccggcaccg ccgctggcgt
     1861 aggtcagcag cgcgatgaag aagatgtcgc ccagaagcag cgacaggagc agcggcgcca
     1921 gtgggtgatc gaacgagtcg cgctggaccc accagaacgc ggccatgctg aacaggaagt
     1981 agagacccgc cgccgtaacg aacgcgttcg gtgcgacgat gcccgcggat cgcaggtcga
     2041 gaaacaaggc ggtgctgagt agcagcgccg cacaaaccgc gcggtagacg ccgacgatcc
     2101 acaggatgcg ccggcccgac tcgttgatca gcgtcggcag cgacgtcggc tcgagtgaag
     2161 ggatcgagga gtcggaagcc atcggattca gcgcttcacg cattgaccgt cggggcacgc
     2221 gtattcgccg gcgcgctgga tagcatcggc gcgcggcagg aagacgccgc agcgggcgca
     2281 gcgcaccatc ggctcgaccg cggtgctgcc ggccgcgcct gcggcgcgct gctgcttgcg
     2341 cgacttgcgc tgattgacca gacgcagcgc gagcagcacg ataaagatca gaaccagcca
     2401 ggcgatgatc ttgcccatga gcttattgcc ggtccttcat cagactacct tgccgggatt
     2461 gagcgtggca tgcgggtcga gcgcccgctt gatccgccgc atcagctcga gctcgatcgg
     2521 cgccttgtaa cgcgccatcg cgtcccgttt catctggccg ataccgtgct cggcgctgaa
     2581 gcttccccca aactcggcga ccagatcgta gacgatcgcc gtggccctgg ccgtatgctc
     2641 gatgaaaacc gcggcgtcga tgctgggcgg
//
LOCUS       DATANS010000157         1795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_134051, whole genome shotgun sequence.
ACCESSION   DATANS010000157 DATANS010000000
VERSION     DATANS010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134051"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1516
                     /locus_tag="VHT22_02290"
     CDS             <1..1516
                     /locus_tag="VHT22_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3136410.1"
                     /translation="LDTWSVPVVMGVILLAGVVYLFMTSKSELAPQEDQGIVLTQIIG
                     PPNATSQQMDVYSKQVFEIAKSIPEYDQMFQITGVPTTNQGFGGVLFKTWSERTKSAT
                     QLQQELQQKWNSVAGARVAAFQFPSLPGSQGLPMQFVIKTTEPYQKLNDVAQAVLAKA
                     QASGMFFYVDTDLKVDKPQTTVHVDRDMVATLGMTQQDVGAALGAALGGGYVNYFSIA
                     GHSYKVIPQVLQEDRLNASQVLDYHIRTADGSLVPASTVATQKTEVVPEGIARFQQLN
                     SATIFGVYSPAVSQNQVLDFMRTTLQQVAPSGYSYDYSGVSRQFVQESGGFVITLLFA
                     VIIVFLALSAQFESFRDPVVILVSVPLALFGALIFINLGLATLNIYTQVGLVTLMGLI
                     SKHGILIVQFANQLQAEGKTKREAIEQAAGVRLRPILMTTAAMVLGVVPLVIAAGAGA
                     AGRHAMGLVIFTGLSIGTLFTLFVVPAVYMLLAADHHKAAATTVEGAPQPQPQP"
BASE COUNT          296 a          578 c          596 g          325 t
ORIGIN      
        1 gctcgataca tggtcggttc cggtggtcat gggggtgata ctgctcgcgg gcgtcgtcta
       61 tcttttcatg acgtcgaagt cggagctcgc gccgcaggag gaccagggca tcgtgctgac
      121 ccagatcatc ggcccgccta acgcgacttc gcagcagatg gatgtctact cgaagcaggt
      181 gttcgagatc gccaagagta ttcccgagta cgaccagatg ttccagatca ccggcgtacc
      241 gaccaccaac cagggcttcg gcggcgtgct gttcaagacc tggagtgagc ggaccaagag
      301 tgccacccag ctgcagcagg agctgcagca gaaatggaac agcgtcgccg gcgcgcgggt
      361 ggccgccttc cagttcccgt cgctgcctgg ctcgcaaggc ctgccgatgc agttcgtgat
      421 caagacgacc gagccctacc agaagctcaa tgacgtcgcg caggcggtgt tggcaaaggc
      481 gcaggccagc gggatgttct tctatgtgga taccgatctc aaggtggaca agccgcagac
      541 gacggtccac gtcgatcgcg acatggtggc gacgctgggg atgacgcagc aggacgtcgg
      601 cgccgcgctc ggcgcggcgc tgggcggcgg ctatgtcaac tatttctcga tcgcaggcca
      661 ctcgtacaag gtgatcccgc aggtgctgca ggaggatcgg ttgaacgcca gccaggtgct
      721 cgactatcac atccgcaccg ccgacggatc gctggtcccg gcgtcgacgg tggcgacgca
      781 gaaaaccgag gtggtccccg agggcatcgc gcgcttccag cagctcaact cggcaacgat
      841 cttcggtgtc tattcgccgg cggtgtcgca gaaccaggtg ctcgacttca tgcggacgac
      901 gctgcagcag gtcgcgccca gcggctactc gtacgactac tcgggcgtct cgcgccagtt
      961 cgtgcaggag tccggcgggt tcgtgatcac cctgctgttc gcggtgatca tcgttttcct
     1021 cgctctatcg gcgcagttcg agagctttcg cgatccggtt gtcatcctgg tgtcggtgcc
     1081 gctggcgctg ttcggcgcgc tgatcttcat caacctcggg cttgcgacgc tcaacatcta
     1141 cacgcaagtc ggactggtga cgctgatggg gctgatcagc aagcacggca tcctgatcgt
     1201 gcagttcgcc aaccagctgc aagccgaagg caagaccaag cgcgaggcga tcgagcaagc
     1261 cgcaggagtg cgcctgcgac cgatcctgat gacgaccgcg gcgatggtgc tcggcgtcgt
     1321 gccgctggtg atcgccgcgg gcgcgggtgc ggccggccgc cacgcgatgg gcctggtgat
     1381 ctttaccggc ctgtcgatcg gcacgctgtt cacgctgttc gtcgtacctg cggtgtacat
     1441 gttgctcgct gcagatcacc acaaggctgc ggcgaccacg gtcgaaggcg cgccccagcc
     1501 gcaaccgcag ccttaggaac ggcgggccgc ggcgccttag tgccgcggcg cgtcgctgcc
     1561 gatgcggtcc ggcgcggcaa atcgcagctg gcgcgtggtg gtgacacgtg gcacggcaat
     1621 tgctcgattg cccttgcccg gggcgggcgg cgcttaccga cgcactcctc gcccttcgcg
     1681 ccggctttgc tccgggccca ccgccattcg gccgatgcgt cgattccacg gttgcgtcgc
     1741 gcggcgcgac cgcgcgctcc ccgctacaca gccagatgcg cgcgcacgtt gtcct
//
LOCUS       DATANS010000158         4400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_470506, whole genome shotgun sequence.
ACCESSION   DATANS010000158 DATANS010000000
VERSION     DATANS010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470506"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1444
                     /locus_tag="VHT22_02295"
     CDS             80..1444
                     /locus_tag="VHT22_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX3136411.1"
                     /translation="MTQREGSLEAPIRHPIDWKNPEYYDQASADKELERIFNICHGCR
                     RCVSLCNAFPTLFDLIDESKTGEIDGVDKADFGKVVDQCYLCDLCYMTKCPYVPPHPW
                     NVDFPHLMLRAKAQKFKRGETNFRDRLLTGTDRLGKLATIPVVVQAVNKANRSPLARK
                     AMDRMLGVHRDRRLPPYATRRFNRLAPDSKDWPVMNGERTPGKVAIFSTCYVNYNEPG
                     IGLDLIAILEHNEIPYALVEKEVCCGMPKLELGDFEAVETLKTANMPKLAGYARDGYA
                     ILTAVPSCTLMFKSELPLLFPGDAETEAVAAAMFDPFEYLVLRDKDGLLKREFKTGLG
                     KVSYHIPCHSRVQNVGQKTREVLEWVPQTTVTTVERCSGHDGTWGVKTEFYQTSMKIG
                     RPVFRQMGEGDPDYVSSDCPIAGRTILQGIEQAQQATRAQKLHPLTLMRIAYGLPGTI
                     EDTR"
     gene            1441..2049
                     /locus_tag="VHT22_02300"
     CDS             1441..2049
                     /locus_tag="VHT22_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEX3136412.1"
                     /translation="MKRIEAERKPRITRDSLLTLEAYARARNEYRAKVIAHKKHRTVH
                     LGEHLTLLFEDELTIRYQIQEMLRIEKTFDEAGIQDELDAYNPLVPDGHNWKATLLVE
                     YEDVDERKTALAKLKGIEDRVWVQVEGAARVYAIADEDLERENETKTSSVHFARFELT
                     PEMIAAVKYGVGISIGVDHPAYTFTLPAISDATRRALVADLA"
     gene            complement(2089..2682)
                     /locus_tag="VHT22_02305"
     CDS             complement(2089..2682)
                     /locus_tag="VHT22_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3136413.1"
                     /translation="MLPERVTSHPVLLRPFTPADALRVQAMLAEAEVAEPTASIPHPY
                     PPGAAAAWIASHDADRERGAQYTYAIASADGATLIGAIALRPLADIHENIGYWIGRPY
                     WGRGYATAATMAITALAFGYLDCEALAASHLERNPASGRVLEKCGFAPLRTVRREHRG
                     RIEAFRVHGITRDAWELRIGGSPGVEMPAEDPTPERS"
     gene            complement(2694..3179)
                     /locus_tag="VHT22_02310"
     CDS             complement(2694..3179)
                     /locus_tag="VHT22_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX3136414.1"
                     /translation="MPSFDIVSEVNQVEVRNAVDQANKEISTRFDFKGSDARVEHKEK
                     ELTLFADDDFKLKQVTDVILGKLTRRGVDVRSLKFGKSEKMSGDKVKQVVTIRVGVEQ
                     ELSKKIVKMLKDSKLKVQASIQGDAVRVSSAKRDELQSAIAAVKTTITDFPLQFGNFR
                     D"
     gene            complement(3183..>4400)
                     /locus_tag="VHT22_02315"
     CDS             complement(3183..>4400)
                     /locus_tag="VHT22_02315"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX3136415.1"
                     /translation="HDVKKVVLAYSGGLDTSVILKWLQDTYGCEVVTFTADIGQGEEL
                     APARAKAAAAGVKQIYIDDLREEFVRDFVFPMFRANAIYEGEYLLGTSIARPLIAKRQ
                     IEIARETGADAVSHGATGKGNDQVRFELGYYALEPDIRVIAPWREWDLSSREKLLAYA
                     EAHGIPIEMKHKAGGSPYSMDANLLHISYEGRALEDPAREAEEDMWRWTVSPEKAPDK
                     PQYLELQYERGDIVAIDGKRMTAANVLSELNRIGGGHGVGRLDLVENRYVGMKSRGCY
                     ETPGGTIMLRAHRAIESICLDREVAHLKDDLMPRYASLIYNGYWWSPERKMMQTMIDA
                     SQATVNGSVRVKLYKGNVDVVGRESSTDSLFDTTIATFEDDRGAYDQKDAAGFIKLNA
                     LRLRIEAKLARRR"
BASE COUNT          799 a         1395 c         1396 g          810 t
ORIGIN      
        1 gtaagttttt gctcgatcgc gcggtcgaga agtttgtatg ccgccggtgc cacatacggt
       61 gcgaacctga aagcctgcga tgacccagcg cgaaggcagc ctcgaagctc cgatccggca
      121 cccgatcgac tggaagaatc cggaatatta cgaccaggcg tcggcggaca aggagctcga
      181 gcggatcttc aacatctgcc atggctgtcg ccgctgcgtc agcctctgca atgcatttcc
      241 cacgctgttc gacctgatcg acgaaagcaa gaccggcgaa atcgacggcg tcgacaaggc
      301 cgacttcggc aaggtcgtcg atcagtgcta tctgtgcgac ctgtgctaca tgaccaagtg
      361 tccgtatgtg ccgccgcatc cgtggaacgt cgattttccg catctgatgc ttcgcgccaa
      421 ggcgcagaag ttcaagcgcg gtgaaacgaa tttccgtgat cgcttgctga ccggcaccga
      481 ccggctcggc aagcttgcga ccataccggt agtcgtccag gcggtgaaca aggcgaaccg
      541 ctcgccgctc gcgcgcaagg cgatggacag gatgctgggc gtccaccgcg accgccggct
      601 gccgccgtat gcgacgcggc gcttcaaccg cctggcgccc gattcgaaag actggccggt
      661 catgaacggc gagcgcacac cggggaaggt ggcgatcttt tcgacctgct acgtcaacta
      721 caacgagccg ggcatcgggc tcgacctgat cgcaatactc gagcataacg agattccgta
      781 cgccctcgtg gagaaggaag tctgctgcgg catgcccaag ctggagctgg gcgacttcga
      841 agcggtcgag acgctcaaga cggccaacat gccgaagctg gccggctatg cgcgcgacgg
      901 ctacgcgatt ctgactgcag tgccgtcgtg cacactgatg ttcaagagtg agctgccgct
      961 tttatttccc ggagacgccg agaccgaagc ggttgccgcg gccatgttcg atccgttcga
     1021 gtatctggtg ctgcgcgaca aggacgggct gctcaaaagg gagttcaaga ccgggctcgg
     1081 caaggtcagc tatcacatcc cctgtcactc gcgagtgcag aacgtcgggc agaaaacccg
     1141 cgaagtgctg gaatgggtgc ctcagacgac ggtcaccacc gtcgagcgct gcagcggcca
     1201 cgacggaacc tggggggtaa agaccgagtt ctaccagaca tcgatgaaaa tcgggaggcc
     1261 ggtgttccgg cagatggggg agggcgatcc cgactacgtc agctccgatt gtccgattgc
     1321 cggccggacg atactgcagg gcatcgagca ggcgcagcag gcaacgcgcg cgcagaagct
     1381 gcatccgctg actctgatgc gcatcgccta cgggctgccg gggacgatcg aggacacacg
     1441 atgaaaagga tcgaagcgga gcgcaagccg cgcatcacgc gcgacagcct gctcacgctg
     1501 gaggcgtacg ccagggcccg aaacgagtat cgcgccaagg tcatcgcgca caagaagcat
     1561 cgcaccgtgc atctgggcga gcacctgacc ctgctgttcg aggacgagct gacgatacgc
     1621 taccagatcc aggaaatgct gcgcatcgaa aagacgttcg acgaagccgg cattcaggac
     1681 gagctcgacg cgtacaaccc gctggtgccg gatggccaca actggaaagc gacgctgctg
     1741 gtcgagtacg aggacgtcga cgagcgcaaa acggcactgg ccaagctcaa ggggatcgag
     1801 gacagggtct gggtgcaggt cgagggcgcg gcgcgtgtct acgcgattgc cgacgaagac
     1861 ctggaacgcg agaacgagac caagacttcg tcggtccatt tcgcgcgctt cgagctgacg
     1921 ccggaaatga tcgccgcggt caaatacggc gtcggcatct cgatcggcgt cgaccacccg
     1981 gcctatacgt tcacgctgcc ggcgatttcc gacgcgacgc gccgcgcgct ggtcgccgac
     2041 ctcgcctagc cgcgcgcgcc gagcggcggc ggccttcgct tccgccggtt acgaccgttc
     2101 cggcgtcggg tcttccgccg gcatctcgac acccggcgag ccgccgatac gcaattccca
     2161 ggcgtcgcgc gtgatgccgt gcacgcggaa cgcctcgatc cgtccgcgat gctctcgtct
     2221 gaccgtgcgc agcggagcga aaccgcattt ctccaggacg cgtcccgatg ccggattgcg
     2281 ctcgaggtgc gaagcggcga gcgcctcgca gtccagataa ccgaacgcca aggcggtgat
     2341 cgccatggtc gcggcggtcg catagccgcg tccccagtac gggcggccga tccagtagcc
     2401 gatgttctcg tgaatgtcgg ccagcggccg cagtgcgatg gcgccgatca aggtcgcgcc
     2461 gtcggcgctg gcaatcgcgt aggtatattg cgcgccgcgc tcgcggtcgg cgtcatggct
     2521 tgcgatccat gctgcggctg cgccgggagg atacggatgc ggtatcgacg cggtcggctc
     2581 cgcgacttcg gcctcggcca gcatcgcctg cacgcgcagc gcatcggcgg gcgtgaacgg
     2641 tcgcaagagg acggggtgcg aagtgacgcg ttccggcagc acacgggcct gtatcagtcg
     2701 cggaaattgc cgaactgcag cggaaagtcg gtgatcgtcg tcttgaccgc cgcgatcgcg
     2761 ctctgcaatt cgtcgcgctt ggcgctggat acgcggacgg catcgccctg gatgctcgcc
     2821 tggaccttga gcttgctgtc cttgagcatc ttgacgatct ttttcgacag ctcctgctcg
     2881 accccgacgc ggatcgtcac gacctgcttg accttgtcgc cgctcatttt ttcgctcttg
     2941 ccgaatttca gcgagcgcac gtcgacgccg cgcctggtga gcttgccgag aatcacatcg
     3001 gtcacctgct tgagcttgaa gtcgtcatcg gcgaacagcg tcagctcttt ctccttgtgc
     3061 tcgacccggg catccgagcc cttgaagtcg aagcgcgtcg aaatctcctt gttggcctgg
     3121 tcgacggcat tccttacttc gacctgattg acttcggaaa cgatgtcgaa cgacggcatg
     3181 gcctacctcc tgcgcgcgag cttcgcctcg atgcgcaggc gcaaggcgtt gagcttgatg
     3241 aatccggcgg cgtctttctg atcgtaggcg ccgcggtcgt cctcgaacgt ggcgatcgtc
     3301 gtgtcgaaca gcgagtcggt gctcgactcg cggccgacga cgtcgacgtt gcccttgtac
     3361 agcttgacgc gtaccgaccc attgaccgtc gcctgcgagg cgtcgatcat cgtctgcatc
     3421 attttccgtt cgggcgacca ccagtagccg ttgtagatca ggctcgcgta gcgcggcatc
     3481 agatcgtcct tcaggtgcgc gacttcgcgg tcgaggcaga tcgattcgat cgcgcgatgg
     3541 gcgcgcagca tgatcgtgcc acccggcgtt tcgtagcagc cgcgcgactt catgccgacg
     3601 tagcgattct cgaccagatc gagtctgccg actccgtgcc ctcctccgat gcgattgagc
     3661 tcggagagca cgttcgccgc ggtcatcctt ttcccgtcga tcgcgacgat gtcgccgcgc
     3721 tcatattgga gctccaggta ttgcggcttg tcaggcgcct tttccggcga cactgtccag
     3781 cgccacatgt cctcttcggc ctcgcgcgcc ggatcctcga gcgcccggcc ctcgtaactg
     3841 atatgcagca ggttggcgtc catcgagtac gggctgccgc cggccttgtg cttcatctcg
     3901 atcggaatgc cgtgcgcctc ggcatacgca agcaactttt cacgcgagct caagtcccat
     3961 tcacgccacg gggcgatgac gcgtatgtcc ggctccagcg cgtagtagcc gagctcgaag
     4021 cgcacctggt cgttgccctt gccggtagct ccatgcgaca cggcgtcggc gcccgtctca
     4081 cgcgcgatct cgatctggcg cttggcgatc agcggacgtg cgatcgacgt cccgagcagg
     4141 tattcgcctt cgtagatcgc gttggcgcgg aacatcggaa agacgaagtc gcgtacgaac
     4201 tcctcgcgca ggtcgtcgat gtatatttgc ttgacgccgg cagccgcggc cttggcgcgc
     4261 gccggcgcaa gctcctcgcc ctggccgatg tcggcggtga aggtgacgac ttcgcaaccg
     4321 taggtgtcct gcagccattt caggatcacc gaggtgtcga ggccaccgga gtaggcgagc
     4381 accactttct tgacgtcatg
//
LOCUS       DATANS010000159         2341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_217938, whole genome shotgun sequence.
ACCESSION   DATANS010000159 DATANS010000000
VERSION     DATANS010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217938"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /locus_tag="VHT22_02320"
     CDS             <1..1543
                     /locus_tag="VHT22_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX3136416.1"
                     /translation="ERAEPRYWDHWLADGREPHVFAADVVSGNARDLLAGTGLALQPW
                     DPSAEHYDLSPDGKELALTADLGSEPRMMNSADIVVVSLATGRHHTLTAGSGFSSEHP
                     RYSPDGKHLAWHAFNLKRAFNDQGRLMLCERASGRTRRLAGRLDRATNHVDWAPDGAA
                     LYMLIEDRGRQGLWRLALTDAMPTQVAAGGTLGGFAVSRDGKRIAFDRASLSHPAALF
                     AIGADGRGELPLESPNRALLDHAAVGVTREFTVKGWGGDPIQVWVTYPPNFDPKKKWP
                     LLHSIHGGPHAAHTDGWHYRWNTQVFAAHGYVVVGVNYHGSSGFGQQWMETITADYGR
                     REFADTEAATDFMLRRGYIDRNRLVATGGSYGGYMVAYMNGHTDRYRTFVCHAGCYDW
                     VSMMATDGYMFFAKELGAFHWDNPARVMQQSPHHFAKRFKTPTLVIHGELDYRVPATQ
                     ALQYYNTLHARQIATRLVYFPDENHWILKPRNSRLWYREFFDWIKRYAAPGPLRRGQR
                     MRLAG"
     gene            complement(1519..1722)
                     /locus_tag="VHT22_02325"
     CDS             complement(1519..1722)
                     /locus_tag="VHT22_02325"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HEX3136417.1"
                     /translation="MKRLSGGNLRSAGYDERKRVLVVELTNGTFEYSGVTAETWRRFA
                     GASSPWSFFRDNIDEDYPAKRIR"
     gene            complement(1798..>2341)
                     /locus_tag="VHT22_02330"
     CDS             complement(1798..>2341)
                     /locus_tag="VHT22_02330"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX3136418.1"
                     /translation="LVDAPVSGGPLRAAAGTMTLIVSGETQAIADCDAVFTAMAARRF
                     VVGEQPGIAATYKVLNNQLAAANLVAGAEAMAVAIRAGIDPHRFLDVVNASSGASWIV
                     DDRMRRALDGDTTSRAATALLNKDVGLAVEVATAFGCEVPLALAARAAFAAAMAAGYG
                     AEDDASLLRLRLGSMAPEPR"
BASE COUNT          389 a          792 c          767 g          393 t
ORIGIN      
        1 cgagcgcgcc gagccgcgct actgggacca ctggctcgcc gatggccgcg agccgcatgt
       61 attcgccgcc gacgtcgtga gcggcaacgc gcgcgatctg ctcgccggca ccggactcgc
      121 gctgcagccc tgggatccgt ccgccgagca ttacgacctg agccccgacg gcaaggagct
      181 cgcgctgacc gccgacctgg gctcggagcc gcggatgatg aattccgcgg acatcgtcgt
      241 ggtcagtctg gcgaccgggc gtcaccacac gctgacggcg ggcagcggat tttccagcga
      301 gcatccgcgc tattcgccgg acggcaaaca tctggcctgg cacgcgttca atctgaagcg
      361 cgccttcaac gatcagggtc gcctgatgct ttgcgagcgc gccagcggcc gcacgcggcg
      421 tcttgccggg agactcgacc gcgctaccaa ccacgtcgac tgggcacccg acggcgcggc
      481 gctgtacatg ctgatcgagg atcgcggccg gcagggacta tggcggctgg ccctgaccga
      541 tgcgatgccg acacaggtgg ccgccggcgg gacactcggc ggtttcgccg tttcgcgcga
      601 cggcaaacgc atcgcgttcg atcgggcgag cctgtcgcat ccggcggcgc tgttcgcgat
      661 cggcgccgac ggccgcggcg agttgccgct ggagtcgccg aatcgtgcgc tgctcgacca
      721 cgccgcggtg ggtgtcacgc gcgaattcac cgtcaagggc tggggcggcg atccgatcca
      781 ggtatgggtg acctatccgc cgaatttcga tcccaagaag aagtggccgc tgctgcattc
      841 gatccacggc gggccgcacg ctgcgcacac cgatggctgg cactaccgct ggaacacgca
      901 ggtcttcgcc gcgcacggtt acgtcgtcgt cggcgtcaat tatcacggct cgtcaggttt
      961 cggccagcaa tggatggaaa cgatcactgc cgattacggc cgccgggaat tcgccgatac
     1021 cgaggcggcg accgatttca tgctgcgccg cggctacatc gatcgcaacc gactggtcgc
     1081 gaccggcggt agctatggcg gctacatggt cgcctacatg aacgggcaca ccgatcgcta
     1141 tcgcaccttt gtctgccacg ccggttgcta cgactgggtc agcatgatgg cgaccgacgg
     1201 gtacatgttt ttcgccaagg agctcggtgc cttccactgg gacaatccgg cacgggtcat
     1261 gcagcagtcg ccccatcatt tcgccaagcg cttcaagacg ccgacactgg tcatccacgg
     1321 cgagctcgac tatcgcgtcc cggctacgca ggcgctgcag tactacaaca cgctgcatgc
     1381 caggcagatt gcaacgcgcc tggtgtattt ccccgatgag aaccactgga tattgaaacc
     1441 gcggaattcg cggctctggt accgggaatt ctttgactgg atcaaacgct atgccgcgcc
     1501 ggggccgttg cgccgcggtc agcggatgcg cttggcgggg tagtcctcgt cgatgttgtc
     1561 gcggaaaaag ctccacggcg aagacgcgcc ggcaaagcgt cgccaggttt ccgctgtgac
     1621 cccagaatat tcgaaggtcc cgttggtcag ctcgacgacc agcacgcgct tgcgctcgtc
     1681 atagccggcg gaacgcaggt tgccgccgga cagtcgtttc atttccatcg cgttggttcc
     1741 gtcgcaccga ctagcggcga ttatagtgag gcgtgcggtc gagcgccgcg ctcgtcacta
     1801 tcgaggctcc ggcgccatcg atccgagacg cagccgcagc agcgaagcgt catcctcggc
     1861 gccgtagccc gcggccatcg cagccgcgaa ggccgcgcgc gcggcgagcg ccagcggtac
     1921 ttcacagccg aacgccgtcg ccacttcgac ggcgaggccg acatccttgt tcaacagggc
     1981 cgtcgccgct cgcgacgtcg tgtcgccgtc gagcgcgcgc cgcatccggt catcgacgat
     2041 ccagctggca cccgagcttg cgttgaccac gtcgagaaag cggtgcgggt cgatcccggc
     2101 gcggatcgcc acggccatcg cttcggcgcc ggcgacgaga ttggccgctg cgagctggtt
     2161 gttgagcacc ttgtaggttg cggcgatccc tggctgctcg ccgacgacaa atcggcgggc
     2221 ggccatcgcc gtgaacacag catcgcaatc ggcgatcgcc tgcgtctcgc ccgacacgat
     2281 cagcgtcatc gtgccggctg ccgcgcgcag gggtccgccg gacaccggcg cgtcgaccag
     2341 c
//
LOCUS       DATANS010000160         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_429190, whole genome shotgun sequence.
ACCESSION   DATANS010000160 DATANS010000000
VERSION     DATANS010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429190"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1588)
                     /locus_tag="VHT22_02335"
     CDS             complement(<1..1588)
                     /locus_tag="VHT22_02335"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX3136419.1"
                     /translation="MGALMRSLDWSTTSIGPVEGWSDALRMMVRVILANRLQMFLWWG
                     SGFCQIYNDAAWPALGAKHPRSMGQPASQCWAEIWHIIGPLIEAPFEGGEATWMDDIF
                     LEMNRKGFTEETHWTIAYSPVPDDTAPNGIGGVIGTVNEITEKVVGQRRIALLRDLGA
                     YSSEAKTPGEACAIAARSLDRHREDVPFGLLYLIQDDGSAMLAGASGISAADAARWDT
                     IDLAAGVSSTQPWPLAAAIHSEAMQVVDDLPAKLASVPAGPWSDPPTLAVVSPIRSNI
                     AHQLVGVMVLGVSSRLVFDDHYRGFFELLTGQVATAIANARAYEAERKRAEALAEIDR
                     AKTAFFSNVSHEFRTPLTLMLGPIEDMMSRVDPHEEVTVGREELDLVHRNSLRLLKLV
                     NTLLDFSRIEAGRMHAAFERVDLAELTGELASVFRSALEKAGLRFIVECQPLDRPVYV
                     DRSMWENIVFNLLSNAFKFTFAGEIEVSLRADQGVELSVRDTGTGIAEHELPRIFNRF
                     HRIAGARGRTFEGSGIGLALV"
BASE COUNT          285 a          538 c          521 g          267 t
ORIGIN      
        1 ataccagcgc gagcccgatt cctgaacctt cgaaagtgcg ccctcgcgcg cctgcgatgc
       61 gatggaagcg gttgaatatc cgcggcagct catgctccgc gatcccggtt ccggtgtcac
      121 gcaccgacag ctcgacgccc tggtcggcgc ggagcgagac ctcgatctcg cccgcgaaag
      181 tgaacttgaa agcgttcgaa agcagattga agacgatatt ttcccacatg ctgcgatcga
      241 cgtacaccgg ccggtcgagt ggctggcact cgacgatgaa gcgcaggccc gctttctcga
      301 gcgccgagcg aaagacgctc gcgagctcgc ccgtcagttc agcgaggtcg acgcgctcga
      361 aggccgcatg catgcgcccc gcctcgatgc gcgagaaatc gagcagggta ttgacgagct
      421 tcagcaggcg caggctattg cgatgcacca gatcgagctc ctcgcggccc accgtgactt
      481 cttcgtgcgg atcgacgcgc gacatcatgt cctcgatcgg accgagcatg agcgtgagcg
      541 gcgtgcggaa ctcgtggctg acgttgctga agaaagccgt cttggcccga tcgatctcgg
      601 ccagcgcctc cgcccgcttg cgctctgcct cgtacgcgcg tgcattcgcg atggcggtgg
      661 cgacctggcc ggtcagcagc tcgaagaagc cgcggtagtg atcgtcgaag acgagccggg
      721 aactcacgcc gagcaccatc acgccaacga gctggtgcgc gatgttggac cggatcggtg
      781 atacgacggc cagggtcggg ggatcggacc acggtcccgc cgggacgctg gcaagcttcg
      841 ccggcagatc atcgacgacc tgcatcgctt cgctgtggat cgccgcggcg agcggccacg
      901 gctgcgtcga ggatacgccg gccgccagat cgatcgtatc ccagcgcgcc gcgtcagcgg
      961 ccgatatccc cgacgcaccc gcgagcatcg cgctgccgtc gtcctggatg agatagagga
     1021 gcccgaaggg cacatcctca cggtggcggt cgagcgaccg ggcagcgatc gcacacgctt
     1081 cgccgggggt cttcgcttcg gaggagtacg ccccgaggtc gcgcagcagt gcgatgcgcc
     1141 gctggcccac gactttttcg gtgatctcgt tcacggtgcc gatgacgccc ccgatgccgt
     1201 tgggcgccgt gtcgtcggga acgggactgt acgcgatcgt ccagtgcgtt tcctccgtga
     1261 accccttgcg gttcatctcg aggaagatgt cgtccatcca ggtcgcttcg ccgccttcga
     1321 acggcgcctc gatgagcgga ccgatgatgt gccagatctc ggcccagcat tggctcgcgg
     1381 gctgccccat cgaccgggga tgtttcgcac cgagtgcggg ccacgccgca tcgttgtaaa
     1441 tctgacagaa cccggaaccc caccagagga acatctgcag acggttcgcg agtatgaccc
     1501 gcaccatcat gcgcagcgcg tcggaccagc cttcgacggg accgatcgac gttgtcgacc
     1561 agtcgagcga gcgcatgagc gcccccatgc gccccccgcc cagcaggcag c
//
LOCUS       DATANS010000161         3854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_50738, whole genome shotgun sequence.
ACCESSION   DATANS010000161 DATANS010000000
VERSION     DATANS010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50738"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VHT22_02340"
     CDS             <1..770
                     /locus_tag="VHT22_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3136420.1"
                     /translation="LEGPYINPGKLGAQPDFVRPASIDEIHALNALAPVRLITLAPEL
                     PGNLDIIVVLRAAGFQVQIGHTLGSYEDGVAALERGAGGFTHLFNAMTGMHHREPGIV
                     GAALAHARYAEIIPDLLHVHPGAIRAALRAIPCLFCVTDSTAATGMPDGDYRLGRHRV
                     TKCLGGVRLADGTLAGSTLTMDQALRNLITIGLSLEDASRRVSTCAAEYLGLPDRGRL
                     AEGVRADLVVVDRDLHLQRVIAEGEELDLAPSSDAVL"
     gene            809..2374
                     /gene="nagZ"
                     /locus_tag="VHT22_02345"
     CDS             809..2374
                     /gene="nagZ"
                     /locus_tag="VHT22_02345"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX3136421.1"
                     /translation="MKQREHSAGRLDPGALVMVGIQGKVLDTEQAAFLRRNRIRAVVL
                     FRSNLGSETEVRALTAALRKAMGPRALIAIDQEGGAVIRATFLPHPPAAMALGAVGSG
                     VLAEEVGAAVARGVKSLGFNWNFAPVADVNNNPANPVIGVRSFSSNPSEVVRLAGAWM
                     RGALREGVACCIKHFPGHGDTRVDSHRELPVVDKSRQALRALELKPFRALQSKAPAIM
                     TAHIVYPRIDSEHPATLSRKLLRGVLREAWGYKGVIITDSLVMEAIQARYGHDRAAVL
                     ALQAGADMVMALGSQDQQAAALRSISGAIDDGSLAADALRRSSLRLDDLAQRYPVRQT
                     RYARTRRESDQELMNRAWALALTPIGDPRPPLKGKPLRVVTQRRVPTDGVSEAGPSGA
                     AVASLFASFGGVDIVQVDDLQRLDWNRLPDDGCATVLVSNQRARYGDASRRWRPQLHL
                     ALWNPFQVLDIRAPAIVTWGFADGALAALRAWLEGRAAAPGHAPVPLALPAPAPQPPT
                     RNRHATAARTTKK"
     gene            2592..>3854
                     /locus_tag="VHT22_02350"
     CDS             2592..>3854
                     /locus_tag="VHT22_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX3136422.1"
                     /translation="MKVIERSITPANEMTVILGALNALRRGDAGVRLPANWLGVSGRV
                     ADVFNEVVERNERMASELERLSRVVGKEGKLGKRGSLGDVTGFWRDSIGAVNLLIDDL
                     VHPTSETARVIGAVAQGDLAQTMALEVDGAPLQGEFLRTAKTVNKMVDQLGSFAAEVT
                     RVAREVGTEGKLGGQAKVKGVAGTWKDLTESVNSMASNLTAQVRNIADVTTAVATGDL
                     SKKITVDVKGEILELKNTINTMVDQLRSFAAEVTRVAREVGTEGKLGGQADVKGVAGT
                     WKDLTESVNSMASNLTGQVRNIADVTKAVAGGDLSKKITVDVKGEILELKNTINTMVD
                     QLSSFASEVTRVAREVGTEGKLGGQAKVKGVAGTWKDLTESVNSMASNLTAQVRNIAE
                     VTTAVATGDLSKKITVDVKGEILELKNTI"
BASE COUNT          710 a         1227 c         1299 g          618 t
ORIGIN      
        1 atctggaagg cccgtacatc aacccgggaa aactcggcgc acagcccgac ttcgtccgcc
       61 cagcatcgat cgacgaaatc cacgcgctca atgcgctggc accggtccgg ctgatcacgc
      121 tggcgccgga gctgccaggc aatctcgata tcatcgtcgt gctgcgcgcc gccggattcc
      181 aggtccagat cggccacacg ctcggcagct acgaggacgg cgtcgccgcg ctcgagcgcg
      241 gcgccggcgg cttcacccac ttgttcaacg cgatgaccgg catgcatcac cgcgagccgg
      301 gcatcgtcgg cgccgcactg gcgcacgccc gctacgccga gatcattccc gatctgctgc
      361 acgtccaccc gggcgcgatc cgcgcggcgc tgcgcgcgat tccctgcctg ttctgcgtga
      421 ccgactcgac cgccgcgacc ggcatgcccg acggcgacta ccggctcgga cgccaccgcg
      481 tgaccaaatg cctcggcggc gtgcgcctcg ccgacggcac gctcgccggc agcacgctta
      541 ccatggatca ggcgctgcgc aacctgatca ccatcggact gagccttgaa gacgcatcgc
      601 ggcgagtatc gacgtgcgcg gccgaatacc tggggctgcc cgaccgcggc cgtttggccg
      661 agggcgtgcg cgccgacttg gtggtcgtcg accgcgacct gcatctgcag cgggtgatcg
      721 ccgaaggtga agagctcgat ctcgcgcctt cgtccgacgc ggtcctgtga ggcgtcaaat
      781 gccaagcggt ccagcgcgac gtgccacgat gaagcagcgg gaacattccg cgggccggct
      841 cgaccccggc gcgctagtga tggtcggcat ccagggcaaa gtactggaca ctgagcaggc
      901 ggccttcttg cgccggaatc gtattcgcgc cgtcgttctc ttccgttcca atctgggcag
      961 cgaaaccgag gtgcgcgcgc tgactgccgc gttgcgcaaa gccatgggcc cgagggcgct
     1021 gatcgccatc gatcaggaag gaggcgcggt catccgtgcg actttcctgc cgcatccgcc
     1081 cgcggcgatg gcgctcggcg cggtggggag tggggttctg gccgaagagg tgggagctgc
     1141 ggttgcacgc ggcgtgaaga gtctcggatt caactggaat ttcgccccgg tggccgacgt
     1201 caacaacaat ccggcgaatc cggtgatagg cgtgcgcagc ttctcgtcga acccttccga
     1261 agtggtgcga ctggccggcg cctggatgcg tggcgcattg cgcgaaggtg tggcctgctg
     1321 catcaagcat tttccgggac acggcgacac ccgcgtcgat tcgcatcggg agctgccggt
     1381 ggtcgacaag tcgaggcagg ccttgcgcgc gctggagctc aagccctttc gcgcgctgca
     1441 gagcaaggca cccgcgatca tgaccgcgca catcgtctat cctcgcatcg attccgaaca
     1501 tccggcgacg ctgtcgcgca agctgctgcg aggtgtgctg cgcgaagcgt ggggctacaa
     1561 aggcgtgatc atcaccgact cgctggtgat ggaggccata caggcgcgct acgggcacga
     1621 tcgtgctgcg gtgctggcgc tgcaggccgg cgccgacatg gtgatggcgc tgggaagcca
     1681 ggaccagcaa gccgctgctc tgcggtcgat ctccggtgcg atcgacgatg gctcgcttgc
     1741 ggccgacgcg ttgaggcgtt catcgcttcg cctcgacgat ctggcccagc gatatccagt
     1801 gcggcagaca cgctacgcgc gaacgcgccg tgagtccgat caagagctga tgaatcgcgc
     1861 ctgggcgctg gcgctcactc cgatcggcga cccgcgcccg ccactgaagg gcaagccgtt
     1921 gcgggtggtc acgcagcgcc gggttccgac cgatggcgtg tctgaagccg gtccctcggg
     1981 cgcggctgtc gcttcgctgt tcgcatcctt cggcggggtc gacatcgtgc aagtcgacga
     2041 cttgcaaaga ttggactgga accggctgcc cgacgacggt tgcgccaccg ttctcgtttc
     2101 caaccagcgc gcgcgctacg gggacgcgtc acggcgctgg cggccgcagc tgcacctggc
     2161 gttgtggaat ccttttcagg tgctggatat tcgtgcgccg gcgatcgtca cctggggctt
     2221 tgccgatggc gcattggcag cgttgcgggc atggctcgaa ggccgcgccg cggcgcccgg
     2281 ccatgcgccg gtgccgctgg ctctgcctgc gcccgcgccg cagccgccga cgcgtaatcg
     2341 ccacgcaaca gccgcccgga cgacaaaaaa ataaatcaac gacttagccg cagtcggtag
     2401 tccacagcgt ttgtaaaagt tacacggtct tagaaattcc cccgatcctc tgccggtatc
     2461 gcctccaaaa ggagaatgat cccgcttgtc gcgtcaatcg ctagcgtgcg caaacttgtg
     2521 cataatttgt cgatcatttg ccgcagcgga cgcgacctga atcacgatcc gactgcaatc
     2581 ggagaattgt catgaaagtc attgaacgct cgataacgcc ggccaacgag atgacggtca
     2641 tcctcggcgc gctgaacgcg ctgcgccggg gcgacgccgg agttcgactg ccggccaact
     2701 ggctcggcgt atcgggacgc gtcgcggacg tgttcaacga agtggtcgag cgcaacgaac
     2761 ggatggcaag cgagctcgag cgtctgtcgc gcgtcgtcgg caaggaaggc aagctcggca
     2821 agcgcggatc gctcggcgac gtgaccgggt tctggcgcga ctcgatcggt gcggtcaacc
     2881 tgctgatcga cgatctggtg catccgacca gcgaaacggc gcgcgtgatc ggcgcggtcg
     2941 cgcagggcga cctggcgcag acgatggcgc tcgaggttga cggcgcgccg ctgcaaggcg
     3001 agtttctgcg caccgccaag accgtgaaca agatggtgga ccagctcgga tcgttcgccg
     3061 cggaagtcac gcgcgtggcg cgcgaggtcg gaaccgaggg caagctcggc ggacaagcca
     3121 aggtcaaggg tgtcgcaggg acctggaagg acctgaccga gagtgtcaac tcgatggcga
     3181 gcaacctgac ggcacaggtg cgcaacatcg ccgacgtgac gaccgccgtc gcgaccggcg
     3241 acctgtcgaa aaagatcacc gtcgacgtca agggcgaaat cctcgagctc aagaacacga
     3301 tcaacacgat ggtggaccag ctgcgctcgt tcgcggcgga ggttacgcgg gtcgcacgcg
     3361 aggtaggcac cgaaggcaag ctcggcggcc aggcggacgt caagggcgtg gcagggacgt
     3421 ggaaggactt gaccgagtcg gtcaactcga tggcgtcgaa cctgaccggt caggtacgca
     3481 acatcgccga cgtgaccaag gcggtggccg gcggcgacct gtcgaaaaag atcaccgtcg
     3541 acgtcaaggg cgaaattctc gagctcaaga acacgatcaa cacgatggtc gaccagttgt
     3601 cgtcgttcgc gtcggaagtc acgcgcgtgg cgcgggaggt cggaaccgag ggcaagctcg
     3661 gcggacaagc caaggtcaag ggtgtcgcag ggacctggaa ggacctgacc gagagtgtca
     3721 actcgatggc gagcaacctg acggcccagg tgcgcaacat cgctgaagtg acgacggcgg
     3781 tcgcgaccgg cgacttgtcg aagaagatca ccgtcgacgt caaaggcgaa attctcgagc
     3841 tcaagaacac gatc
//
LOCUS       DATANS010000162         1500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_638797, whole genome shotgun sequence.
ACCESSION   DATANS010000162 DATANS010000000
VERSION     DATANS010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638797"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="VHT22_02355"
     CDS             <1..863
                     /locus_tag="VHT22_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136423.1"
                     /translation="GYALFQEGGVRHLLHPSDTTTGVHYSSLAMIHAILEDLLTPEQA
                     RKHLQLLDTHLRGPDGVRLFDKPMPYHGGPMRLFQRAESATFFGREIGLMYMHAHLRY
                     AQALAHVGEADRFFHALCQANPIGIRAVVSPATLRQANCYYSSSDAAFADRYEASAEY
                     QRVVEGRVDLDGGWRIYSSGAGIALGLIVRRFLGLSVEANALSVDPVIPSALDGLQLT
                     TTLLGKPTEVRYAVGAKGCGVSRIELNGQTLQFERERNPYREGAARVAIDAVREALQA
                     KKNDLEIVLG"
     gene            complement(908..>1500)
                     /locus_tag="VHT22_02360"
     CDS             complement(908..>1500)
                     /locus_tag="VHT22_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136424.1"
                     /translation="QEDLSAVAQREVWSKLGVNPGGVAADVVWRTNAQRPATRPGSTL
                     QYRELASGILANTNAMARVGLLFLRQGKWKDQQILSEAFVTQVHTPVPENASLPVEPD
                     LPGHLFPNAQTDYGVLWWTNKSHTMANVPTDTFWAWGLGEELIVVIPSLDLVIVRNGG
                     QSATNPAGRVWNDGDWDGDVRVLEPFLNPIVAGTTP"
BASE COUNT          262 a          480 c          496 g          262 t
ORIGIN      
        1 caggttatgc gttgtttcag gagggtggcg tgcgccacct cctgcatccc agcgacacga
       61 cgacgggcgt tcactacagc tcgttggcga tgatccacgc gatcctcgaa gacctgctga
      121 cgccggaaca agcgcgcaag catctgcagc tgctcgacac gcatctgcgc ggtcccgacg
      181 gcgtgcgctt gttcgacaag ccgatgccgt atcacggcgg accgatgcgc ttgttccagc
      241 gcgccgagag cgccacgttc ttcggccgcg agatcgggct gatgtacatg cacgcgcatc
      301 tgcgctacgc gcaggcgctt gcgcacgtag gcgaagcgga tcgcttcttc cacgcgcttt
      361 gccaggccaa cccgatcggc atccgcgccg tcgtctcgcc cgcgacgctg cggcaagcga
      421 actgctacta ctcgagctcc gatgccgcgt tcgcggatcg ctacgaagcg agtgcggagt
      481 atcagcgtgt agtcgaagga cgagtcgatc tcgacggcgg ctggcgcatc tattccagcg
      541 gcgccggcat cgcgctggga ttgatcgtgc gccgattcct cgggctgagc gtggaagcga
      601 atgcactcag cgtggatccg gtcattccgt cggcgctcga cggcctgcag cttacaacga
      661 cgctgctcgg caagccgacc gaggtgcgct acgcggtggg cgcgaaggga tgcggcgtca
      721 gcaggatcga gttgaacggg cagacgttgc agttcgagcg cgagagaaat ccgtatcgcg
      781 agggagctgc gcgcgtcgcg atcgacgcag tgcgcgaggc actgcaggcg aagaagaacg
      841 atctggagat agttttgggc tagtgggctg gctgccggcg atgagaccgg cagccagccc
      901 ctgaccgtta cggcgtcgtt cccgcgacga tcggattcaa gaacggctcc agcacgcgga
      961 cgtcgccgtc ccagtcgccg tcattccaga cgcggccagc gggattggtc gccgactggc
     1021 cgccgttgcg tacgatcacg agatcgaggc tcggaatcac cacgatcagc tcttcgccca
     1081 ggccccaggc ccagaacgta tccgtcggca cgttcgccat ggtgtgggac ttgttcgtcc
     1141 accacagcac gccatagtcg gtctgcgcgt tggggaacag gtgaccaggg aggtcaggct
     1201 cgaccggcag actggcattc tccggaaccg gcgtgtgcac ctgggtaacg aatgcctcgc
     1261 tcaggatctg ctggtccttc cacttacctt ggcgcaggaa cagcaacccg acgcgcgcca
     1321 tcgcgttggt attggccagg atgccggacg caagctcgcg atactgcaga gtgctgccgg
     1381 gacgtgtggc cggacgctgc gcattcgtgc gccagacgac gtcggcggcc acgccgcccg
     1441 ggttgacacc gagcttggac cacacttctc gctgtgccac ggcgctcagg tcctcctgat
//
LOCUS       DATANS010000163         2174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_261058, whole genome shotgun sequence.
ACCESSION   DATANS010000163 DATANS010000000
VERSION     DATANS010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261058"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..605)
                     /locus_tag="VHT22_02365"
     CDS             complement(33..605)
                     /locus_tag="VHT22_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136425.1"
                     /translation="MLAPAHVGHLAMLRSLIRQNAAEGSFDSGLAADSTQSVEFFDKL
                     KRALVHGYFIEEDLQTGRVESVAVPGYVFWPDDRHSGNSPTGFGLFRAIAGGFELWLA
                     ALELGQRGGGHGRTMLDALFATAPGRKTWVVRIPRDSRYRAAVEHLLATHSFDRVGDT
                     NRLRWFLRSDAPSALAINVRRAVNGNPPRN"
     gene            complement(777..>2174)
                     /gene="polA"
                     /locus_tag="VHT22_02370"
     CDS             complement(777..>2174)
                     /gene="polA"
                     /locus_tag="VHT22_02370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006761695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX3136426.1"
                     /translation="RRLDIKTLSYAEITGKGASQISFDQVSIERATEYAAENADITVQ
                     LHRAQHPRVAADAGLDFVYSSIEMPTSEVLFRMERTGVLLDCALLDAQSRELGERVLA
                     LENRAYELAGQPFNLGSPKQLGDILFGKMGLPVVKKTATGQPSTDEDVLQQLAADYPL
                     PKLLLEHRGLSKLKSTYTDKLPLMRNPRTGRVHTSFGQATAVTGRLASTDPNLQNIPV
                     RTSEGRRIREAFIAPPGHTIVSADYSQIELRIMAHLSGDESLLKAFAEGADIHRATAA
                     EIFGVPLADVSGDQRRYIKAVNFGLIYGMSAFGLAAQLAIERSAAQMFIDKYFTRYPG
                     VAEYMRRTRELARKQGYVETVFGRRLWLPDINAGGGPRRQGAERAAINAPMQGTAADL
                     IKLAMIVLQQWLDREQLGTRLILQVHDELVLEAPDAELDRVKRELPVLMSGVAQLRVP
                     LVVDVGSGPNWEQAH"
BASE COUNT          379 a          697 c          706 g          392 t
ORIGIN      
        1 gcttcagcgc gcggacgcgc ttgatgcgcc agctaattgc gcggcggatt gccgttgacg
       61 gcgcggcgga cgttgattgc gagcgccgac ggggcgtcgc tgcgcagaaa ccagcgcagc
      121 cggttcgtgt cgccgacgcg atcgaatgag tgcgtggcca gcagatgctc gaccgccgct
      181 cgatagcgtg aatcccgcgg tatgcgcacg acccaggttt tcctcccagg cgccgtcgcg
      241 aacagcgcgt cgagcatcgt gcggccatgg ccgccgccgc gctgtccgag ctccagcgcg
      301 gctagccaga gctcgaagcc accggcgatc gcacgaaaga ggccgaagcc cgtcggcgag
      361 tttccgctgt ggcgatcgtc gggccagaac acataaccgg gcaccgcgac cgactcgacc
      421 ctgccggtct gcaggtcttc ctcgatgaaa tagccgtgga cgagtgcgcg cttgagcttg
      481 tcgaagaact cgaccgattg cgtggaatcc gcagcgagtc cggaatcgaa actgccttcg
      541 gcggcattct gtcggataag cgaccgaagc atggccagat gaccgacatg ggcgggtgca
      601 agcatgtttg caaaccacgc gacgggagca ggcgctgttt atagaccctg gcatgtctgg
      661 ccgctggtcc agcttacgaa cggatagcgc agcagcacta acggttgtgc cgtcacaaac
      721 ggcgagcgat gtgcacgaca acatccgtcc aaggcaagaa caatcgtcag cagccgctag
      781 tgcgcctgct cccagttcgg accgctgccg acatcgacga ccagcggcac gcgcagttgc
      841 gcaacgccgc tcatcagcac gggcaactcg cgcttgaccc ggtcgagctc tgcgtcgggc
      901 gcctcgagca ccagctcgtc gtgcacctgc agaatcagcc gggtgccgag ttgctcgcga
      961 tcgagccatt gctgcaagac gatcatcgcg agcttgatca agtcggccgc ggtgccctgc
     1021 atcggcgcat tgatcgccgc acgctcggcg ccctggcgac gcggaccgcc gcctgcgttg
     1081 atgtcgggca gccagagccg ccgcccgaac accgtttcga catacccttg cttgcgcgcc
     1141 agctcgcgcg tcctccgcat gtactcggcc acgcccggat agcgagtgaa gtacttgtcg
     1201 atgaacatct gcgccgcgct gcgctcaatc gcgagctgcg ctgcaaggcc gaacgcgctc
     1261 atgccgtaga tcaggccgaa gttgacggcc ttgatgtagc gccgctggtc gccgctgacg
     1321 tcggcgagcg gcacgccgaa tatctcggcc gccgtcgctc ggtgaatgtc ggcgccttcg
     1381 gcgaaagcct tgagcagcga ttcgtcgccg gacagatgcg ccatgatgcg cagctcgatc
     1441 tgcgaatagt cggccgagac gatcgtgtgt ccgggtggcg cgatgaatgc ttcgcgtatc
     1501 cgcctgccct cggacgttcg caccggtatg ttctgcagat tgggatcggt gctggcgagc
     1561 cgtccggtga ccgctgtcgc ctggccaaaa ctggtatgaa cgcgcccggt gcgcgggttg
     1621 cgcatcagag gcagcttgtc ggtataggtc gacttgagct tggacagccc ccggtgctcg
     1681 agcagaagct tcggtagtgg gtagtcggcg gccagctgct gcagcacgtc ctcgtcggtc
     1741 gacggttgtc cggtcgccgt cttcttcacg accggcagcc ccatttttcc gaacaggatg
     1801 tcgccgagct gtttcggcga tccgaggttg aacggctggc cggcgagctc gtaggcgcgg
     1861 ttttcgagcg cgagcacgcg ctcgcccagc tcgcgactct gtgcgtccag caatgcgcag
     1921 tcaagcagca cgccggtgcg ctccatccga aacagcactt cgctggtcgg catctcgatc
     1981 gacgaataga cgaaatcgag gcccgcatcg gcggcgactc gcggatgctg ggcccgatgc
     2041 agctggacgg tgatatcagc attctccgcc gcgtattcgg tagcgcgctc gatcgacacc
     2101 tggtcgaaac tgatttgcga ggcacccttt ccggtgatct ctgcatagct aagggttttt
     2161 atgtcgagcc ggcg
//
LOCUS       DATANS010000164         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_470764, whole genome shotgun sequence.
ACCESSION   DATANS010000164 DATANS010000000
VERSION     DATANS010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470764"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1289
                     /locus_tag="VHT22_02375"
     CDS             <1..1289
                     /locus_tag="VHT22_02375"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX3136427.1"
                     /translation="FPKSPGKGKASDAKPRPDDAGARPDAGARSDARSPPGSARELAA
                     TMKARPAAVLTRGEPRPSAAENNEREITVTGPPSLIEWTPGLNQKIQVAEANPGLCAV
                     LENAALLYANGNAAHARLVLEDGIANDADAKTSPLAWLALFDLLQRLGDYAEYDRLSL
                     QYVVAFERSAPSREELGIQTRQGARPVAAGYVGLTGKLNAGHAPQIANLLAASQKQAQ
                     VRLDLGSLTGADNAGAKLLADALAQLRRRKYPLALQHPEKIRNALEHSVVQGRAAGEG
                     YWMLLLELLQWQNDHKVFEDRAVEFAVAFEVSPPSWEPPPSSQPAEAAGDSAAPADAV
                     TGNPEMLVWQGTLLGASDPQLAKLAMFGEGRHAIPIDMGGIDRIDFVCAGAIFNAISR
                     AETQRKSIQIVGASPIIRALLLLIGISPRHFIKKAT"
     gene            1380..1916
                     /gene="hslV"
                     /locus_tag="VHT22_02380"
     CDS             1380..1916
                     /gene="hslV"
                     /locus_tag="VHT22_02380"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX3136428.1"
                     /translation="MDLFHGTTILSVRRGAKVALGGDGQVTLGNVVIKATARKVRRIY
                     NDRVLAGFAGATADAFTLFERFEAKLDKHQGQLQRAAVELAKDWRSDRILRRLEAMLA
                     VADATSSLVITGTGDVLEPEYGIVAIGSGGAYAQAAARALLEHTEIPADDIVKQALTI
                     AGDICIYTNQNHVIEVLG"
BASE COUNT          346 a          684 c          676 g          327 t
ORIGIN      
        1 tgtttccgaa gtccccgggc aagggcaaag cttccgacgc gaagccccgc cccgacgacg
       61 cgggcgcacg ccccgacgcg ggagcgcgct ccgatgcgcg ctctccgccc gggtcggcgc
      121 gggagctcgc ggcgacgatg aaggctcggc ccgcggcggt gctgacgcgc ggcgagccgc
      181 gcccctccgc ggctgagaac aacgagcgcg agatcaccgt caccggcccg ccgagcctga
      241 tcgaatggac tccgggactc aatcagaaga tccaggtcgc cgaagccaat ccgggtctct
      301 gcgccgtgct cgagaacgcg gcgctgctgt acgccaatgg aaacgccgcc catgcccgct
      361 tggtgctcga ggacggcatc gccaacgatg ccgacgccaa gacttcaccg cttgcctggc
      421 tcgcattgtt cgatttgctg cagcgcctgg gcgactacgc cgagtacgac cgactgtcgc
      481 tgcaatatgt cgtcgctttc gagcgctcgg ctccgtcgcg cgaagagctg ggcatccaga
      541 cgcgccaggg cgcgcgtccg gtggccgccg gttacgtcgg cctgaccggt aagctcaacg
      601 ccggacatgc gccgcagatc gcgaacctgc tcgcggcgtc acaaaagcag gcgcaggtgc
      661 gtctcgatct gggctcgctg acgggcgccg acaacgcagg ggcgaagctc ttggccgatg
      721 cgctggcgca gttgcgcagg cgcaaatatc cgctggcgct gcaacatccg gaaaagatac
      781 gaaacgcact cgagcattcg gtcgtgcagg gtcgcgccgc tggagaaggt tactggatgc
      841 tattgctcga attgctgcag tggcagaacg accacaaggt gttcgaggat cgcgccgttg
      901 aattcgccgt cgcgttcgaa gtatcaccgc cgtcctggga gccgccgccc tcgtcgcagc
      961 ccgcagaggc tgccggcgat tccgccgcgc cggccgacgc ggtgacgggg aatccggaaa
     1021 tgctcgtctg gcagggaacg ctgctgggcg cgtcggaccc gcagctcgcc aagctcgcga
     1081 tgttcggcga aggacgacat gcgataccga tcgacatggg cggaatcgac cgtatcgatt
     1141 tcgtctgcgc gggagcaatt ttcaatgcca tcagccgcgc cgagacgcag cgcaagtcga
     1201 ttcagattgt cggcgcatcg ccgattatcc gggcgttgct gcttctgatc ggcatctcgc
     1261 ctcggcactt catcaagaaa gcgacgtagc agcggttccc aggaggtcat ggcgcggcga
     1321 ctgcgctttc gccgcgcatc cgcgccgacg caaccgcacc gactccgctg catccgacaa
     1381 tggatctctt tcacggtacg acgatacttt ccgtgcgccg cggcgcaaag gtcgcgcttg
     1441 gcggcgacgg ccaggtcacg ctcggcaacg tcgtcatcaa ggcgaccgcg cgcaaggttc
     1501 gccgcatcta caacgatcgc gtgctcgccg gctttgcggg cgcgacggcc gacgcgttca
     1561 ccctgttcga gcgcttcgaa gccaagctcg acaagcacca gggacagctg caacgcgctg
     1621 ccgtcgaact ggcgaaggac tggcgctccg atcgcatcct gcgccggctc gaggcgatgc
     1681 tggcggtggc cgacgcgacc tcttcgctgg tgatcaccgg caccggcgac gtgctcgagc
     1741 cggagtacgg cattgtcgcg atcggctcgg gtggtgccta tgcccaagcg gccgcccgcg
     1801 ccctgctcga gcacaccgag attcctgccg acgacatcgt caagcaggcg ctgacgatcg
     1861 ccggcgacat ctgcatctac accaatcaga atcacgtgat cgaggtgctc gggtgagcgc
     1921 ggatgccggc gtgcgcgacg tgatcatcgt cggcgccggc ctggtcggcc ttgcgcttgc
     1981 ggcggcgcgg gcgcgtaccg gcttgagcgt catgatcgct gatcgcggca gcg
//
LOCUS       DATANS010000165         2332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_302829, whole genome shotgun sequence.
ACCESSION   DATANS010000165 DATANS010000000
VERSION     DATANS010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302829"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /gene="trpB"
                     /locus_tag="VHT22_02385"
     CDS             complement(<1..1097)
                     /gene="trpB"
                     /locus_tag="VHT22_02385"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX3136429.1"
                     /translation="MQMADVTYALPDESGHFGPYGGVFVAETLIHALDELREQYARYR
                     TDPEFVAEFEYELKHYVGRPSPVYHARRWSERLGGAQLYFKREDLNHTGAHKINNCIG
                     QALLARRMGKRRVIAETGAGQHGVAAATVAARFGMDCVVYMGSEDVRRQAQNVFRMKL
                     LGAELVPVESGSKTLKDALNEAMRDWVTHVEDTFYIIGTVAGPHPYPMMVRDFQTVIG
                     RECLTQMLEMTGRQPDAVIACVGGGSNAIGIFHPYIPYDKVRLIGVEAAGDGISTGRH
                     AASLTAGRPGVLHGNRTYLLQDANGQIVETHSVSAGLDYPGVGPEHAWLKDSGRAEYV
                     TVTDDEALAAFHDCCRIEGIIPALESCHALA"
     gene            complement(1084..1755)
                     /locus_tag="VHT22_02390"
     CDS             complement(1084..1755)
                     /locus_tag="VHT22_02390"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX3136430.1"
                     /translation="MKPAPRTRVKICGITRIDDALAAANAGADAIGLVFWPGTPRKVE
                     FEQARAIAAAVPAFVTIVGLFVDPQPAAVRAAVDAVPLDVLQFHGDEPQALCASFARP
                     YIKAVPVRQDVDLLQYASRYRDARGLLFDAFVPGDMPGGTGRTFDWQALATRLGAGLA
                     RPLILSGGLTPHNVGAAIRALRPWAVDVSSGVETTGDDGRPHKGIKDPAKIAAFIRGV
                     RDADG"
     gene            complement(1772..>2332)
                     /locus_tag="VHT22_02395"
     CDS             complement(1772..>2332)
                     /locus_tag="VHT22_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HEX3136431.1"
                     /translation="RGVNALLPSSVAVRWGLGMPADFHARFAATGRHYTYLLLDRPVR
                     PALLAGRVGWYHRPLALDLMRTAADALLGRHDFSAFRAAECQAKSATRMLDRLDIARE
                     DDMIRFDFHADAFLQHMVRNIVGALVYVGNGRHSPGWIAELLAGRDRTRAAPTFAPDG
                     LYLTGIDYPPRWNLPSTHAPARAFGT"
BASE COUNT          407 a          780 c          765 g          380 t
ORIGIN      
        1 tgcgccagcg catgacacga ttcgagcgcg ggaatgatgc cctcgatgcg gcagcagtcg
       61 tgaaacgccg ccagcgcttc atcatcggtc accgtcacat attcggcgcg cccgctatcc
      121 ttgagccagg cgtgctcggg gcctacaccc ggatagtcga gaccagccga caccgaatgg
      181 gtctctacga tctgaccgtt cgcgtcctgc agaagatagg tccggttgcc gtgcagcacg
      241 ccgggtcgtc cggcggtcag cgaggccgcg tgccggccgg tagagatgcc gtcgccggcg
      301 gcctcgacgc cgatcagcct gaccttgtcg tacgggatgt acgggtggaa gatcccgatc
      361 gcgttcgacc cgccgccgac gcaggcgatc accgcatccg gctggcggcc ggtcatttcc
      421 agcatctgcg tcaggcattc gcggccgatg accgtctgga aatcgcgcac catcatagga
      481 tagggatgcg gtccggcgac ggtaccgatg atgtaaaacg tatcttcgac gtgcgtcacc
      541 cagtcgcgca tagcctcgtt cagcgcgtcc ttcagcgtct tcgagcccga ttcgactggc
      601 accagctcgg cacccaggag cttcatccgg aacacgttct gcgcctggcg tctgacgtcc
      661 tcggacccca tgtatacgac gcaatccatg ccgaagcgtg cggcgacggt cgcggcagcg
      721 acgccgtgct ggccggcacc ggtctcggcg atcacgcgcc gcttgcccat gcgccgcgcc
      781 aacagcgcct gtccgatgca gttgttgatc ttgtgcgcac cggtgtggtt caggtcctcg
      841 cgtttgaaat aaagttgcgc gccgccgagt ctttccgacc atctcctggc gtggtagacc
      901 gggctcggcc ggccgacgta gtgcttgagc tcgtactcga attcggcgac gaactcggga
      961 tcggttcgat agcgggcata ctgctcgcgc agttcgtcga gagcgtgaat cagcgtctcg
     1021 gcgacgaaca cgccgccgta cggtccgaag tgaccgctct cgtcgggaag cgcgtacgtt
     1081 acgtcagcca tctgcatcac gcactcctcg gatgaacgcg gcaatcttcg ccgggtcctt
     1141 gatgcctttg tgcggtctgc cgtcgtcgcc ggtagtctct acgccgctcg atacgtcgac
     1201 cgcccacggg cgcaatgccc ggatcgcggc gccgacgttg tgcggcgtga gcccaccgga
     1261 cagaatcagc ggtcgcgcga gcccggcgcc gagcctcgtc gccagggcct gccaatcgaa
     1321 ggtccggccg gtaccgcccg gcatgtcgcc gggaacgaac gcatcgaaca acaggccccg
     1381 cgcatcgcga taacgcgaag cgtattgtag caaatcgacg tcctgccgca cgggaacggc
     1441 cttgatgtaa ggccgtgcga acgacgcgca cagcgcctgc ggctcatcgc cgtggaattg
     1501 caggacgtcg agcggcaccg cgtcgacggc cgcgcgcacc gctgcgggct gcggatcgac
     1561 gaacaggccg acgatcgtga cgaacgccgg caccgcggcc gcaatcgcgc gcgcctgctc
     1621 gaattcgacc ttgcgcggcg taccaggcca gaaaaccagt ccgatggcat cggcgcccgc
     1681 gttcgccgcc gccagcgcat cgtcgatgcg cgtgatgccg catatcttga cgcgcgtgcg
     1741 cggtgcgggc ttcatcgtgc gtgcatcgct ttcaggtgcc gaacgcacgg gccggcgcgt
     1801 gcgtcgaggg aagattccac cgcggcggat agtcgatgcc ggtgaggtac agaccgtcgg
     1861 gagcgaacgt cggcgcggcg cgcgtgcgat cccggccggc cagcaattcg gcaatccacc
     1921 ccggcgaatg gcggccgttg ccgacataga ccagcgcgcc cacgatgttg cgcaccatgt
     1981 gctgcaaaaa ggcatcggcg tgaaaatcaa atcggatcat gtcatcctca cgcgcgatat
     2041 cgagccggtc gagcatccgg gtggccgatt tcgcctggca ttcggcggcg cgaaaggccg
     2101 aaaaatcgtg tctgcccaga agtgcatcgg cggcggtccg cataaggtcg agcgcaagcg
     2161 gccgatggta ccagccgacg cggccggcga gcagcgccgg ccgcaccggc cgatcgagca
     2221 gcagatacgt atagtgcctg ccggtcgcgg cgaagcgcgc gtggaaatcc gccggcatgc
     2281 cgagccccca gcggactgcg accgatgaag gcagcaacgc gttgacgccg cg
//
LOCUS       DATANS010000166         2349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_302887, whole genome shotgun sequence.
ACCESSION   DATANS010000166 DATANS010000000
VERSION     DATANS010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302887"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VHT22_02400"
     CDS             complement(<1..430)
                     /locus_tag="VHT22_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3136432.1"
                     /translation="MKLYGFPPSPNTRKVQAVAAHLNMPLEFEFVDITQGKSHTPEFL
                     KMNPTGRTPVFTHGDFTLWESNAIMQYLASQRKNDLWPDDARTRADISRWQLWQVAHW
                     HEGCGGFLWENLVKKALGMGEGDPAALKKSEEAFHRDAAML"
     gene            complement(620..>2349)
                     /locus_tag="VHT22_02405"
     CDS             complement(620..>2349)
                     /locus_tag="VHT22_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX3136433.1"
                     /translation="EKANWFWSGADCEEPAKRRCMIGLSDGGEDAVALREFDLRSARF
                     VDGGFSLPRGKQHAAWQDADTLLVAREWSPGELTRSGYPFVVKRIKRGQPLSSAVEVF
                     RGTASDVGVSPSALNDGAGHRALVIRRSVSFFEAEYQLVRSTGTSKLALPSKADIVAL
                     VGGRLVVNLREDWTTDAGTFPQGSLVSIDLAAASATPERPRPTLIYAPGPREAFQQAA
                     ATRGHLLVSVLDNVKGRAYVYKPEPAGKWSRRRIALPDNVSIHIVDADLHSDHAFLSV
                     TGFLTPTSLWLADVATGALATVKTLPPRFDASHHVVEQFEAASSDGTRVPYFVVHRID
                     MKLDGSTPTILTAYGGFLVSQTPYYAPQTGKLWLERGGALVLANIRGGGEFGPAWHDA
                     GLKTNRQRIFDDFTAVARDLIAKGITSPAHLGIEGGSNGGLLMGVQFIQHPELWGAVD
                     MQVPLLDMLGYEHIAAGSSWVGEYGSVSNPDERAFLASISPYDNLKPGVRYPEALIWT
                     TTKDDRVGPQHARKFAARLAAMGVPYLFYEVVQGGHASGATLAERAAMTAREFTYFWR
                     KLAPSENPR"
BASE COUNT          402 a          750 c          769 g          428 t
ORIGIN      
        1 cgagcatcgc ggcgtcgcga tgaaacgcct cttccgactt tttcaacgcg gcggggtctc
       61 cttcgcccat gccgagagcc ttcttgacca ggttctccca caggaatccg ccgcagccct
      121 cgtgccagtg ggcaacctgc cagagctgcc atcggctgat atcggcgcgc gtgcgcgcgt
      181 cgtcgggcca caggtcattt tttcgctggc tggccaggta ctgcatgatc gcgttcgatt
      241 cccatagcgt gaaatcaccg tgggtaaaca ccggcgtccg cccggtgggg ttcatcttca
      301 ggaactcggg cgtatgcgac ttgccttgag tgatgtcgac gaactcgaac tccagcggca
      361 tgttcagatg cgcggcgacg gcctggacct tgcgcgtatt gggcgatggg ggaaatccgt
      421 acagcttcat gtctgcctcc ttggatggtt atcaccatgg tagatgggag atgacaatcg
      481 gttaaccaat aggttaagta ttagggaaac cgatcgccgt gtcaagcggg caggtccttg
      541 cggaggaccc gatcgcggcg cgtgtgcgcc ggagcgtcag cgcaacgtca gctggttgcg
      601 tcgattatcc gggttgcgct cagcggggat tttccgacgg cgcaagcttg cgccagaaat
      661 aggtgaattc gcgtgcggtc atcgccgctc tctcggccag ggtcgccccc gaggcgtgcc
      721 ctccctggac gacctcgtag aagagatagg gcacgcccat cgccgccagc cgggctgcga
      781 atttgcgcgc gtgctgcgga ccgacccgat catccttggt cgtcgtccag atcagcgctt
      841 ccggatatcg aacgcccggc ttcagattgt cgtacggcga tatcgacgcc aggaacgccc
      901 tttcatcggg attcgagacg ctgccgtact cgccgaccca ggagctgcct gccgcaatgt
      961 gctcgtagcc cagcatgtcg agcagcggga cctgcatgtc gacggcgccc cacagctcgg
     1021 gatgctgaat gaactgcact cccatcaaca ggccgccgtt ggagccgccc tcgatgccga
     1081 ggtgggccgg actggtgatg cccttcgcga tcaaatcgcg cgcgacggcc gtgaagtcgt
     1141 cgaagatgcg ctgtcggttc gtcttgagac cggcgtcgtg ccatgcgggc ccgaattcgc
     1201 ctccgccgcg gatgttggcc agcaccagcg cgccgccgcg ttccagccaa agtttgccgg
     1261 tttgcggcgc atagtacggt gtctgggaaa ccaggaaacc gccgtaggcg gtgagtatcg
     1321 tcggcgtgct gccgtcgagc ttcatgtcga tgcggtgaac gacaaaatac gggacgcgcg
     1381 tcccatcgct cgacgcggcc tcgaactgct cgaccacgtg atgtgaagcg tcgaaccgcg
     1441 gcggcagcgt cttcacggtg gcaagcgcgc cggtcgccac gtccgcgagc caaaggctgg
     1501 tcggcgtcaa aaagccggtg acgctcaaga atgcgtgatc gctgtgcagg tccgcgtcga
     1561 cgatgtgaat cgaaacgttg tcgggcagcg cgatcctgcg tcgcgaccac ttgcccgcgg
     1621 gctcgggctt gtagacgtag gcgcgtccct tgacgttgtc gagcacgctg accagcaagt
     1681 ggcctcgcgt ggcggctgcc tgctggaagg cttcgcgtgg acccggagcg tatatcagcg
     1741 tcggccgcgg tcgttcgggc gttgcgctcg ccgcggcgag atcgatcgac accagcgatc
     1801 cctgcgggaa tgttccggcg tcggtcgtcc agtcttcgcg gaggttgacg accaggcggc
     1861 cgccgacgag cgcaacgatg tcggccttcg acggcaacgc cagcttgctc gttccagtcg
     1921 agcggaccag ttgatattcg gcttcgaaga acgataccga gcggcggatg acgagtgccc
     1981 ggtgcccggc gccgtcgttc aaggccgacg gactgacgcc gacgtcgctg gccgttccgc
     2041 gaaacacctc gacggcggac gaaagcggct gcccgcgctt gatgcgcttg acgacgaacg
     2101 ggtagccgga gcgagtgagc tcgccgggcg accattcccg cgcgaccagc agcgtatcgg
     2161 cgtcctgcca ggccgcgtgc tgcttgccgc ggggaagcga aaagccgcca tcgacgaatc
     2221 gcgccgagcg caaatcgaac tcccgcagcg caaccgcgtc ctcgccgccg tccgacaagc
     2281 cgatcatgca gcggcgcttc gcaggctctt cgcaatcggc gccgctccag aaccagttgg
     2341 ccttctccg
//
LOCUS       DATANS010000167         2629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_218554, whole genome shotgun sequence.
ACCESSION   DATANS010000167 DATANS010000000
VERSION     DATANS010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218554"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VHT22_02410"
     CDS             complement(<1..428)
                     /locus_tag="VHT22_02410"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX3136434.1"
                     /translation="MTLARIRGAYEKFLEAIVIVLMSAMAIEVTIGIGFRAFGRSLSW
                     YDEVAVILLAWLTFYGSALAALKRAHIGFPGLVKSMPPRRRLVFVVIGKFLVIAFMAL
                     LGWIGFSILDVLATDYLINFPAVSVAWTQSVIPISCALFIV"
     gene            complement(455..1420)
                     /locus_tag="VHT22_02415"
     CDS             complement(455..1420)
                     /locus_tag="VHT22_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012179986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX3136435.1"
                     /translation="MSRLLWIGLLAVFSQPALAQLELKFGHVGEPGSLFDVSATEFAK
                     RANAKLAGKATIVVYGSSQLGGDKEMLQKLKLGTLDFALPSTVMSSEADLFGLFEMPY
                     LVKDRGHMQKIEKAIFWPKLEPAAEAKGLRILAVWENGYRHITNSKKPIVTPADLQGI
                     KLRIPEGKWRLRMFQAYGANPSPMKLSEVFTALSTGVMDGQENPLTQIWSQKFQEVQK
                     FLSLTGHVYTPAFVAVGAKRWDSLPADVRKVLEDTAKETQAFVYERAKKDDDELVAKF
                     TAAGVKVNQADKDAFIAASKGIYDEFGAEVSGSKALIEQAIALGK"
     gene            complement(1494..>2629)
                     /locus_tag="VHT22_02420"
     CDS             complement(1494..>2629)
                     /locus_tag="VHT22_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX3136436.1"
                     /translation="DHPTIDHRGPEFARMTLAILDGLKKVFACDGPVVIYPASGTGAW
                     EAALVNTLSAGDRVLMFETGHFATLWRKMADKLGLSVDFVPGDWRSGADAAVVESKLA
                     DDKAHAIKAVLVVHNETSTGVTTAIPPIREAIDRAGHPALLMVDTISSLASIDYRHAE
                     WGVDVTVAGSQKGLMLPPGLSFNAVSDKALAASKEARLPRSYWAWDDIIAMNRQGYFP
                     YTPATNLLYGLHEAIDMLLGEGLPNVFARHDRHAEATRRAVRGWGLEILCRNPAEYSS
                     SLTAIIVPEGHDADAFRREALARYDLSLGTGLGRLAKKVFRIGHLGFFNDLTLCGTLC
                     GVEMALAASRVPHKSGGIAAALSFLAGASSIKSGAATLAASTA"
BASE COUNT          469 a          861 c          819 g          480 t
ORIGIN      
        1 acgatgaaca gtgcgcaact gatcgggatg accgactgcg tccacgcgac ggagactgcc
       61 ggaaagttga tcagatagtc ggtcgccagt acatcgagga tcgaaaagcc gatccacccc
      121 agcagcgcca tgaaggcgat caccaggaac ttgccgatga ccacgaagac cagccgccgg
      181 cgcggcggca tcgacttgac cagtcccggg aacccgatgt gcgcgcgctt cagcgccgcc
      241 agcgccgatc cgtagaaggt gagccaggcg agcaggatga cggcgacctc gtcgtaccac
      301 gacagcgaac ggccgaacgc gcgaaacccg atgcctatgg tcacctcgat cgccatcgcc
      361 gacatcagca cgatgacgat cgcttcgagg aatttctcgt atgcgccacg aattcgcgcc
      421 aacgtcacgc tcgctcccct cgcccgtcgg acgcttattt gcccagcgcg atcgcctgct
      481 cgatcagcgc tttcgagccg gagacttcgg cgccgaactc gtcgtagatc cccttgctcg
      541 ccgcgataaa cgcgtccttg tcggcctggt tgactttgac gccggcagcg gtgaacttgg
      601 cgaccagctc gtcgtcgtcc tttttggcgc gctcgtagac gaacgcctgc gtttctttcg
      661 ccgtgtcctc gaggaccttg cgcacgtcgg cgggcaggct gtcccagcgc ttcgcaccga
      721 cggcgacgaa cgccggcgtg tagacgtggc cggtcagcga aagaaacttc tgcacttcct
      781 ggaatttctg cgaccagatc tgcgtcagcg gattctcctg gccgtccatg acgccagtcg
      841 acagcgcggt gaacacttcg gaaagcttca tcgggctggg gttcgcgccg tacgcctgga
      901 acatcctgag ccgccatttg ccttcgggta tgcgcagctt gatgccctgc agatcggcgg
      961 gtgtgacgat cggcttcttg ctgttggtga tgtggcggta gccgttttcc cagacggcaa
     1021 ggatcctgag tcccttcgct tccgccgccg gctcgagctt cggccagaag atcgcctttt
     1081 cgatcttctg catgtggccg cgatccttga ccaggtacgg catttcgaac aggccgaaca
     1141 ggtcggcctc cgacgacatc accgtcgaag gcagcgcgaa gtcgagcgtg ccgagcttga
     1201 gcttctgcag catctccttg tcgccgccga gctggctcga gccgtagacc acgatcgtcg
     1261 ccttgcccgc cagcttggcg ttggcgcgct tggcgaattc agtcgccgag acgtcgaaca
     1321 gcgatcccgg ttcgccgaca tggccgaact tgagctccag ctgcgccagc gcgggctggc
     1381 tgaacacggc aagcaacccg atccacaaca gtctcgacat cctcatggcg attcctccga
     1441 agttcaaaca caatcagtta tttttttatt gcttcgactt tcgatgcgac gcgtcaggcc
     1501 gtgctggcgg cgagcgttgc agcgccggat ttgatgctcg acgcgccagc gagaaacgat
     1561 agcgccgcag cgatgccacc cgatttatgc ggaacgcgcg aggccgccag cgccatctcg
     1621 acgccgcaca gcgttccgca cagcgtcagg tcgttgaaga atcccaggtg accgatgcga
     1681 aacacctttt tcgcaaggcg gccgaggccg gtgccgagcg acaggtcgta gcgcgcgagc
     1741 gcttcgcggc ggaaagcatc ggcgtcgtga ccctcgggaa cgatgatcgc ggtcagcgag
     1801 ctgctgtatt cggccgggtt gcggcacagg atttccagcc cccagccgcg caccgcgcgc
     1861 cgggtcgctt cggcgtgtct atcgtggcgc gcaaacacgt tcggcaagcc ttcgccgagc
     1921 agcatgtcga tagcctcgtg cagtccgtag agcaggttgg tcgccggcgt gtacggaaaa
     1981 tagccctgcc ggttcatcgc gatgatgtcg tcccaggccc agtacgatcg cggcagccgc
     2041 gcctccttgc tggcggcgag cgccttgtcg ctgaccgcat tgaacgagag ccccggcggc
     2101 aacatcagcc ctttttgcga gccggcgacg gtgacgtcga cgccccactc ggcgtgccgg
     2161 taatcgatcg aggccagcga cgagatcgtg tcgaccatca acagcgcagg atggccggcg
     2221 cggtcgatcg cctcgcggat aggcgggatc gcggtggtga cgccggtcga cgtctcgttg
     2281 tgcacgacca gcaccgcctt gatcgcgtgc gccttgtcgt ccgcgagctt ggattcgacg
     2341 accgcggcgt cggcgccgct gcgccaatcg ccgggaacga agtcgaccga gaggccgagc
     2401 ttgtcggcca tcttgcgcca aagcgtcgcg aagtggcccg tctcgaacat cagcaccctg
     2461 tcacccgcgg acaaggtgtt caccagcgcc gcttcccagg cgccggtccc cgatgcgggg
     2521 tagatcacga ccggcccgtc gcaggcaaac acttttttca aaccgtcgag gatggcgagc
     2581 gtcatccgcg cgaactccgg tccacggtga tcgatcgtgg ggtggtcga
//
LOCUS       DATANS010000168         1982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_177724, whole genome shotgun sequence.
ACCESSION   DATANS010000168 DATANS010000000
VERSION     DATANS010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177724"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="VHT22_02425"
     CDS             <1..969
                     /locus_tag="VHT22_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX3136437.1"
                     /translation="LGTNNCRLLVARPSGEGFRVVDAFSRIVRLGEGVSASGQLSEPA
                     IARAIAALAICRNKMKNKGVTRARLIATEACRAAQNGVQFLARVADELGLELEVIDRE
                     TEARLAATGCTPLIDPQAEGAILFDIGGGSSEVVRLARSHRGRGGPPLPQIRGWISLP
                     HGVVTLAERYGGITVTRETYEAMVSEVASLIAPFVREHGGDGTNGIHMLGTSGTVTTI
                     AGVHLNLKRYDRNRVDGCWMNADQITTVIERLLAMTHEDRVASPCIGIERADLVLAGC
                     AILEAIRRAFPCPRLRVADRGLREGMLVQMMREDGAWSGGNQAAMS"
     gene            979..1674
                     /locus_tag="VHT22_02430"
     CDS             979..1674
                     /locus_tag="VHT22_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEX3136438.1"
                     /translation="MANPRQKKVRVRGGKNRSVSSRQWLQRQLNDPYVARAKREGMRS
                     RAAYKLMEIDDKARFLRKGARVVDLGAAPGGWSQVAAQRVSATEQGRVIAIDILAMEP
                     VPGVDFIALDFLDRTAPDKLKDMIGGPADVVLSDMAANATGHARTDHLKIMALVEAAA
                     EFAREVLAPGGTFLAKVLQGGTEAALLASLKRDYKTVKHVKPPASRTDSAELYLLATG
                     FRGASAAASQPAR"
     gene            complement(1659..>1982)
                     /locus_tag="VHT22_02435"
     CDS             complement(1659..>1982)
                     /locus_tag="VHT22_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3136439.1"
                     /translation="LQFTSINTIAYAEVDPRRIGRATALVSVAQQLAVSSGVALGALT
                     VELTVHFRGGGPLQAEDFPPAFLAVAAISALSALIFARLAPDAGAEMADRLPTPAEPS
                     DQRAG"
BASE COUNT          359 a          609 c          677 g          337 t
ORIGIN      
        1 ctcggcacga acaactgtcg gcttctggtg gcgcggccgt caggcgaggg gttccgcgtc
       61 gtcgatgcgt tttcacgcat cgtcaggctt ggcgagggcg tctcggcgtc cggccagttg
      121 agtgagcctg cgatcgcgcg cgcgatcgcg gcgctcgcca tttgccgcaa caagatgaaa
      181 aacaagggcg tgacccgcgc acggctgatt gcgaccgaag cctgccgcgc cgcgcagaac
      241 ggcgtgcagt ttctcgcccg tgtcgccgac gaattgggtc tcgagctcga ggtgatcgac
      301 cgcgaaacgg aagcgcggct cgcggccacc ggctgcacgc cgttgatcga cccgcaggcc
      361 gagggtgcca tcctgttcga tatcggcgga ggttcgtcag aagtcgtacg gcttgcgcgc
      421 tcgcacaggg gacgtggcgg gccaccattg ccgcagattc gcggctggat ttccttgccg
      481 cacggggtcg tcacgttggc cgagcgctac ggcggaatca cggtcacccg ggaaacctac
      541 gaggcgatgg tcagcgaggt cgcatctttg atcgcgccct tcgtgcgcga acatggcggg
      601 gacgggacga acggaatcca tatgctcgga acgtccggca cggtgacgac gatcgcgggc
      661 gtgcacctca acctcaaacg ttatgatcgc aatcgagttg acggttgctg gatgaacgcg
      721 gaccagatca ccaccgtcat cgagcgattg cttgcgatga cacacgagga tcgggtggcg
      781 agcccctgca ttggaatcga acgcgcagat cttgtgctcg ccggctgcgc gatcctcgag
      841 gcgatccggc gcgcgttccc gtgcccgcgc cttcgcgttg ccgaccgcgg tttgcgcgag
      901 gggatgctgg tgcagatgat gcgcgaagac ggtgcctgga gcggcggcaa ccaggcggcc
      961 atgtcctgac cggcaccgat ggcaaatcca cgtcaaaaaa aggttcgtgt tcgcggcggc
     1021 aaaaatcgtt cggtgtcgtc gcgtcaatgg ctgcaacgtc agctcaatga tccttacgtc
     1081 gcgcgcgcga agcgcgaggg catgcgctcg cgcgccgcgt acaagctgat ggaaatcgac
     1141 gacaaggcgc gatttctcag gaaaggcgcg cgcgtggtcg atcttggcgc ggcaccgggc
     1201 ggctggagtc aggttgccgc gcaacgcgtt tcagcaaccg agcagggccg ggtcatcgcg
     1261 atcgacattt tggcgatgga gcccgtgccg ggggtggact tcatcgcgct cgatttcctc
     1321 gatcgcaccg cgccggacaa gctcaaggac atgattggcg ggccggccga cgtcgtgctc
     1381 tccgacatgg cggccaatgc aacaggacac gcgcgcactg atcacctcaa gatcatggcg
     1441 ttggtcgaag cggccgccga attcgctcgc gaggtgctgg cgcccggcgg cacgtttctc
     1501 gcaaaagtgc tgcaaggtgg caccgaggcg gcactgcttg cctcgctcaa gcgggactac
     1561 aagaccgtca agcacgtcaa gccgccggca agccggacgg attcggctga attgtatctg
     1621 ttggcaacgg gatttcgcgg cgcgtccgcc gccgcatctc agccggcgcg ctgatcggaa
     1681 ggctccgccg gggttggcag tctgtcagcc atctcggcac cggcgtccgg tgccagccgg
     1741 gcaaagatca gcgccgagag agcggaaatc gccgcaaccg ccagaaatgc cggcggaaaa
     1801 tcctcggcct gcagaggtcc gccacctctg aagtggacgg tcaattcgac agtcagggct
     1861 ccaagagcga cgccggatga cacagcgagc tgctgcgcga cgctgaccag cgctgtcgcg
     1921 cggccgatgc gccgtggatc gacctcggca taggctattg tgttgatgct ggtgaattgc
     1981 ag
//
LOCUS       DATANS010000169         2324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_596005, whole genome shotgun sequence.
ACCESSION   DATANS010000169 DATANS010000000
VERSION     DATANS010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596005"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1646)
                     /gene="dnaX"
                     /locus_tag="VHT22_02440"
     CDS             complement(<1..1646)
                     /gene="dnaX"
                     /locus_tag="VHT22_02440"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX3136440.1"
                     /translation="MSYQALARKWRPKRFADLVGQDHVVRALTNALERGRLHHAYLLT
                     GTRGVGKTTIARLLAKSLNCATGVTATPCGVCPACTDIDAGRFVDLLELDAASNTGID
                     NMREILDNARYAPTVGRYKVYLIDEVHMLSKAAFNSMLKTLEEPPEHVKFILATTDPQ
                     KIPVTVLSRCLQFNLKVLPAAQIAARLADILRAEDIAFDDSALALIAHSARGSMRDAL
                     SVLDQAIAYGAGEVREAVVRTMLGAVDAEYAYRIVDALLAGDGKALLAEAATLSQRSL
                     SLPAALDELASLFHRIAVAQTVAGVADALPDAARIAGYATAFTPDAVQLAYQICVQGR
                     ADMALAPDEQTGFAMTLLRLLAFEPAAGPSAETKAPVRAVAREAASTSGPRTSSAPAS
                     GSTQGASGNAQVASGSRATGGAPLAAIDPPAETRVASAPAQSSAATLAPLQIADWPAF
                     VAGLKLSGIALQLAAQTALKAIAGNEIVLAVPEAARHLIDKTYTDKLKAALDEAFGRR
                     VRLRFDVDAAAGATLAAQEKRERAEVKAKTEAAFRDDPFV"
     gene            complement(1851..1949)
                     /gene="ffs"
                     /locus_tag="VHT22_02445"
     ncRNA           complement(1851..1949)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VHT22_02445"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT          380 a          772 c          765 g          407 t
ORIGIN      
        1 tgcacgaacg gatcgtcgcg aaacgcggct tcggtcttgg ccttcacttc ggcgcgctcc
       61 cgcttttcct gcgccgcgag cgtcgcgccc gccgccgcgt cgacatcgaa acgcaggcgg
      121 acgcggcgtc cgaacgcctc gtccagggcc gccttcagct tgtcggtata agtcttgtcg
      181 atcagatgtc gcgcggcttc cgggaccgcg agcacgattt cgttgccagc gatggctttg
      241 agcgcggtct gcgccgcgag ttgcagcgcg atgcccgaca gcttcagacc ggcgacgaaa
      301 gccggccagt cggcgatctg caacggcgcg agcgtcgctg cgctcgactg ggctggcgcg
      361 gacgcgacgc gggtctcggc tggcgggtca atcgccgcca gtggcgctcc gcccgtcgcg
      421 cggctgccgc ttgcaacctg cgcgttgccg ctggcgcctt gcgtgctgcc gctcgccggc
      481 gcgctgctcg tcctcggccc gctcgtgctc gcggcctcgc gagcgacggc gcgcacgggc
      541 gccttcgtct ccgccgaagg ccccgcggcg ggctcgaacg cgagcaggcg caacaaggtc
      601 atcgcgaatc ccgtctgctc gtccggcgca agcgccatgt cggcccggcc ctgaacgcag
      661 atctggtagg caagctgcac ggcgtccgga gtgaacgccg tggcatagcc ggcgattcgc
      721 gccgcgtcgg gcagcgcatc ggcgacaccc gcgacggtct gcgcgacggc gatgcggtga
      781 aacaggctcg ccagctcatc cagcgccgca ggcagcgaaa ggctgcgttg gctcaacgtc
      841 gcggcttcag ccagcaaggc cttgccatcg cccgcaagca aggcatcgac aatgcgatac
      901 gcgtactcgg cgtcgaccgc accgagcatc gtgcgcacca cggcttcccg cacctctcct
      961 gcgccgtagg cgatcgcctg gtcgagcacc gagagggcat cgcgcatcga gccccgcgcg
     1021 ctatgcgcga tcaaagccag cgccgagtcg tcgaacgcga tgtcctcggc gcgaagaata
     1081 tcggccagcc gcgccgcgat ctgcgcggcc ggaagcacct tcaggttgaa ttgcaggcag
     1141 cgcgaaagca cggtgaccgg gatcttttgc ggatcggtcg tggcaaggat gaatttgacg
     1201 tgctcgggag gctcctcgag cgttttaagc atcgaattga acgcggcctt cgacagcatg
     1261 tgcacttcgt cgatcaggta gaccttgtat ctgccgaccg tcggcgcgta gcgtgcgttg
     1321 tcgagaatct cccgcatgtt gtcgatgccg gtattcgacg ccgcgtcgag ctcgagcaga
     1381 tcgacaaagc gaccggcgtc gatatcggtg cacgcagggc atacgccgca gggcgttgcc
     1441 gtgacgccgg tcgcgcaatt gaggctcttc gctaggagcc ttgcgatcgt cgtcttgccg
     1501 acgccgcgcg tgccggtaag cagatacgcg tgatggagac gtcctcgttc gagcgcgttg
     1561 gtcagcgccc gcacgacatg gtcttgcccg accagatcgg caaatcgctt cggtcgccat
     1621 ttgcgggcga gcgcttggta gctcatcggg ctttcagcgt ttcgttcagc aaagcattgc
     1681 gcgatttcgc ttcgcgttcg cgttgcagcg aaccgcgagg ctatttcgcg tcgcgttcgg
     1741 tttcaggcaa ggcgctcgac cgacgcaggc gcgccgtgta cccaaaatgt acataagccc
     1801 ctgcggcggg cgggcaacgt agcatgaagc cgaactgacg cgaaagaggg ggtggcgagc
     1861 cggacccccg gcacttgcag agaatagcta tggctgcttc cttccggacc tgaccaggtt
     1921 caccaccatg caatgcgggg cggcccgcca ttgatcgttg ccgctgctgc ccctcattat
     1981 cggcgaagca agccgatgca gcaagcaacg cgacggtgcg agactcgatg cactgcggat
     2041 tcacccgtcc gcctggccgt catcggcggg ccggttataa tgcaatcagc gcatcatgca
     2101 ttggagtcgg attgcgcaca cgccttctgc agcgtatctg catcgcggtc tcgatgacag
     2161 tggttgcctg cagtccatcg caacccccgc cgccgccttc gtcgaacgaa ttgaccgtgc
     2221 tgctgcgcac cgggccgggg acctggttcg tcggcgccga tgaccaaggc gcgggcatcg
     2281 actatgacct tgcgcacatg ttcgccgagc aggagggaac gacg
//
LOCUS       DATANS010000170         1622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_51371, whole genome shotgun sequence.
ACCESSION   DATANS010000170 DATANS010000000
VERSION     DATANS010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51371"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VHT22_02450"
     CDS             <1..894
                     /locus_tag="VHT22_02450"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX3136441.1"
                     /translation="AVIPGPDSTTPPQLNLIPSEERPRAGSWRRWQVWVPLTLLAAVA
                     LAALLVPLVQKREYAIALNKQAEEARVQALAADAVRTQLERAQGEYNFVLAKKYAYPG
                     TIQLLDGVTRVLPDDTWVTQLEMRTSLRGKETQRDLVLRGESANAGKLIGLLEDSKLV
                     EQAALRSSTTKIQPGPGEIFDLGAQVKKLPLPAPVPLSAIPDAAAAPAPPAPPPPAPA
                     AATPVPSNAAAPTPSAPPPGGRTAAPGVQAQTAAPARAAPPAPAIVQPAATPPGMAPG
                     ATPAAPGSPPAAPASDDEETD"
     gene            894..1490
                     /gene="gspM"
                     /locus_tag="VHT22_02455"
     CDS             894..1490
                     /gene="gspM"
                     /locus_tag="VHT22_02455"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HEX3136442.1"
                     /translation="MATLALPTSPRSSRALAVLLLVVAVVLGLALLIAPVLLLHRHYD
                     VAIEGTLDHLERYRRVAAQAPETKKALETLRAKNGRRFFLKNTAPNLAGAELQDLVRA
                     AIENNGGRITTVQIVAPKEDGRFRQIGVNVQLFASTPNLQKILYAVETQQPYLVVEGL
                     TIRPLNAFRGFKPAPGVEPEVSVLLDIVAFSNTGAEKP"
BASE COUNT          270 a          567 c          544 g          241 t
ORIGIN      
        1 gctgtcattc cgggtccgga ctcgacgacg ccgccgcaac tgaacctgat tccgtcagag
       61 gagcggccgc gcgccggcag ctggcggcgc tggcaggtct gggtcccgct gacgctcctc
      121 gccgctgtcg cgctggcagc actgctggtc ccgctggtgc aaaagcgcga atacgcgatc
      181 gctctgaaca agcaggccga agaagcgcgc gtgcaggcgc tggcggccga cgccgtgcgc
      241 acgcaactcg agcgggcgca gggggagtac aacttcgtcc tggcaaaaaa atacgcctat
      301 ccgggcacga ttcagcttct cgacggggtc acgcgtgtgc tgcccgacga cacctgggta
      361 acgcagctcg agatgcgaac cagcctgcgc ggcaaggaga ctcagcgcga cctggtgctg
      421 cgcggcgaat cggcgaacgc tggcaagctg atcggcctgt tagaagattc caagctggtc
      481 gagcaggcgg cgctgcgatc gtcgaccacc aagattcagc cgggacccgg agagattttc
      541 gatctcggcg cacaggtgaa gaagcttcct ctgcctgcgc cagttccgct gtcagcaata
      601 cccgatgctg cggcggcgcc cgcgccccct gcgccgccgc ctccggcccc cgccgctgcg
      661 acgcccgtcc cgtcgaacgc cgcggcgccg accccatccg ctccgcctcc tggcgggcgc
      721 actgcggcgc cgggtgtcca ggcgcagacc gcggcacccg cgcgcgcggc gccaccagcg
      781 ccggctatcg tccagcccgc cgcaacgccg cctggcatgg cgcccggcgc gacacccgca
      841 gccccggggt cgccgccggc ggcgccggcc agcgacgatg aggaaaccga ctgatggcaa
      901 cgctcgcgct gcccacttcg ccgcgctcga gccgcgcact cgcggtgttg ctgctcgtcg
      961 tcgccgtcgt actggggctg gcgctgctga tcgcgccggt gctgctgctg caccggcact
     1021 acgacgtcgc gatcgaaggc acgctcgatc acctggaacg ctatcggcgg gtcgccgcgc
     1081 aggcgccgga gaccaagaaa gcgctcgaga cgctgcgcgc gaagaacggc cgccgtttct
     1141 ttctgaagaa tactgcgccg aatcttgccg gtgccgagtt gcaggacctg gtgcgggcgg
     1201 cgatcgagaa caacggcgga cgcatcacga ccgtgcaaat cgttgcgccc aaggaagatg
     1261 ggcgtttccg ccagatcggc gtcaacgtcc agctgttcgc atcgacaccc aacctgcaga
     1321 agatccttta tgcggtcgag acgcagcagc cctacctggt cgtcgaaggc ctgacgatac
     1381 ggccgctgaa cgcgtttcgc gggttcaagc cggcgcctgg cgtcgagccc gaggtcagcg
     1441 tgctgctcga catcgtcgca ttctccaaca ccggggcaga aaagccatga acggaaatct
     1501 tcgcgcgttg ctatggtggg tcgcgcccgt gctggcgttt gtcgcactgg tgggcatcga
     1561 gaccgactgg ggccaccagc tacggagggt gcctgcggcg gctcaggcga tcgagcccaa
     1621 gc
//
LOCUS       DATANS010000171         3340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_134753, whole genome shotgun sequence.
ACCESSION   DATANS010000171 DATANS010000000
VERSION     DATANS010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134753"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1340
                     /locus_tag="VHT22_02460"
     CDS             <1..1340
                     /locus_tag="VHT22_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX3136443.1"
                     /translation="TLEDDAASKQGARCMDCGIPFCQSGCPINNIIPDWNDLVYRQQW
                     HEALEVLHSTNNFPEFTGRICPAPCEAACTLNINDDPVGIKSIEHFIIDKGWEEGWVA
                     PQPPKRKTGKRVAVVGSGPAGMACAQQLGRAGHEVVLFEKADRIGGLLRYGIPDFKME
                     KYHIDRRVEQMRAEGVEFRVNAHVGATGKNGVPAARLLEEFDALVASAGSEHPRDLDV
                     PGRDLDGVHYAMDFLPLQNRRVAGDTDVPPLTAKGRHVIVIGGGDTGSDCVGTSNRQG
                     ASSVTQFELLPQPPEHENKPLVWPYWPMKMRTSSSHEEGCERDWAVQTKAFTGEGGKV
                     VALVASRVEWKDGKLRDVPGSEFELKADLVLFAMGYLHPVHAGMLEELGVEYDARGNV
                     KADTEHYRTSVPKLFAAGDTRRGQSLVVWAIREGRQCARAVDEFLMGASELPR"
     gene            1356..2231
                     /locus_tag="VHT22_02465"
     CDS             1356..2231
                     /locus_tag="VHT22_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HEX3136444.1"
                     /translation="MTDIAALDVASWRPALSHDAQDAAIRTLEGGGVLILPQLAFPLA
                     ADEQRFLSPRWSDGRAKNISFDGGKLKGARGSADELAALSRMVARFAADASGLVTAMF
                     PRYAPYVRRARTSFRPQPAVGRAVSWRKDDSRLHVDAFPSRPNHGERILRVFCNVSPS
                     EDRVWRVGESFEAVARRFLPGVRRQIPGEARWLSALHVTKGLRSPYDHLMLGLHDGAK
                     ADLAYQANCAQQVVRFAPGTTWLCFSDQLMHAAVSGQYMLEQTIDLPLAALYDRERSP
                     LAILERLCGRELVAG"
     gene            2385..>3340
                     /gene="hemE"
                     /locus_tag="VHT22_02470"
     CDS             2385..>3340
                     /gene="hemE"
                     /locus_tag="VHT22_02470"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX3136445.1"
                     /translation="MTPLQNDTLLRALRREPTDYTPIWLMRQAGRYLPEYNATRARAG
                     SFMALCTNPQLAAEVTMQPLARFALDAAILFSDILTVPDAMGLGLHFVDGEGPRFARP
                     LVDEEAVGKLAVPDPERLRYVYDAVATIKRALAGKLPLLGFAGSPFTRACYMIEGGGS
                     ADFARVRRMLYARPDLLHRVLSVNAEAVAAYLAQQVAHGADALMLFDTWGGLLTTEAY
                     TRFSLAYIRTVLERVRQSTGAVTPTIVFTKGGGQWLERIADTGCSAIGVDWTTDIAAA
                     RARVGARVALQGNLDPLALLATPDVVRAEAAAVLDAATTSPG"
BASE COUNT          540 a         1100 c         1123 g          577 t
ORIGIN      
        1 tgacgctcga ggacgacgcc gcgtcgaagc agggcgcgcg ctgcatggac tgcggcatcc
       61 cgttctgtca gtccggctgt ccgatcaaca acatcattcc cgactggaat gacctggtct
      121 atcggcagca gtggcacgag gcgctcgaag tgctgcactc gaccaacaac tttccggaat
      181 tcaccgggag aatctgcccg gcgccatgcg aagctgcctg cacgctcaat atcaacgacg
      241 acccggttgg catcaagtcg atcgagcact tcatcatcga caagggttgg gaagaaggct
      301 gggtcgcgcc gcagccgccg aaacgaaaga ccggcaaacg cgtggccgtg gtcggttccg
      361 gcccggccgg aatggcatgc gcgcagcagc ttgggcgcgc cggccacgaa gtcgtgctgt
      421 tcgagaaggc tgacagaatc ggcggcctgt tgcgctacgg aattcccgac ttcaagatgg
      481 agaagtacca catcgaccgc agggtcgagc agatgcgagc cgaaggagtc gagttccgcg
      541 tcaatgcgca tgtcggcgca acgggaaaga acggcgtccc ggcggcgcgc cttctcgagg
      601 aattcgacgc gctggttgcc tcggcgggct ccgagcatcc gcgcgatctg gatgtccccg
      661 gtcgtgacct cgacggtgtt cattacgcga tggacttcct gccgctgcaa aacaggcgcg
      721 tcgccggcga caccgacgtt ccgccgctta ccgcaaaagg ccggcacgtc atcgtcatcg
      781 gcggtggcga taccggctcg gactgcgtcg gcacatcgaa tcggcagggc gcgtcgtcgg
      841 tgacgcagtt cgagttgctg ccgcagccgc ccgagcatga gaacaagccg ctagtctggc
      901 cgtactggcc gatgaagatg cggacgtctt cgtcgcacga ggaaggctgc gaacgcgatt
      961 gggcggttca gacgaaggcc ttcacgggcg agggtggcaa ggtggttgcg cttgtcgcca
     1021 gccgcgtcga atggaaggac gggaagcttc gcgatgtgcc cggcagcgaa ttcgagctca
     1081 aggccgacct ggtgctcttc gcgatgggtt atctgcatcc ggtgcacgcc gggatgctcg
     1141 aagagctggg cgtcgaatac gacgcgcgcg gcaacgtcaa ggccgacacc gagcattatc
     1201 gaacgtcggt cccgaagctg tttgccgctg gcgacacgcg ccgcggccag tcgctggtcg
     1261 tctgggcaat acgcgagggc cgccagtgcg cgcgcgccgt cgacgaattc ctgatgggcg
     1321 cgtccgagct gccgcgttag gacacaggtt cctccatgac cgatatcgcc gccctcgacg
     1381 tcgcgagctg gcggccggcg cttagccacg atgcacagga cgccgcgata cgaacgctcg
     1441 aaggcggcgg cgtgctgatc ttgccgcagc ttgcattccc gctggccgcc gacgagcagc
     1501 gcttcctttc gccgcgctgg tccgacggca gggccaaaaa catcagcttc gacggcggca
     1561 agctcaaggg cgcgcgcggc tcggccgacg agctcgcggc gctgtcgcgc atggttgcgc
     1621 gattcgcggc cgacgcctcg ggcctggtca cagcgatgtt tccacgttat gcgccgtacg
     1681 ttcgccgcgc gcgcaccagc ttccgtccgc agcccgccgt cgggcgcgcg gtttcctggc
     1741 gcaaggacga ctcgcgcctg cacgtcgacg catttccatc gcggccgaat catggcgagc
     1801 gcattctgcg cgtgttctgc aatgtcagcc ccagcgagga tcgggtatgg cgcgtcggcg
     1861 agagcttcga agcggtggcg cgcaggtttc tgccgggcgt tcgccggcag atccccggcg
     1921 aggcgcgttg gctgtcggcg ctgcacgtga ccaagggcct gcgcagcccg tacgatcacc
     1981 tgatgctcgg cctgcacgac ggcgccaagg cagatctcgc ctaccaggcg aattgcgcgc
     2041 agcaggtcgt gcgcttcgcc cccggtacca catggctgtg cttttccgac cagctgatgc
     2101 atgcggcggt gtcgggccag tacatgctcg agcagacgat cgatcttccg ttggccgcgc
     2161 tgtacgatcg cgagcgatca ccgctggcga tcctggaacg gctttgcggc cgcgagctcg
     2221 tggccggctg aagcgtcgca tcgcgtcgat cgccgcctcg tccggcggcg aagctgcgaa
     2281 ggcgaagccg gtccgccgcg attcggctct acaatagcgg ccgtgccgcc aggactcgac
     2341 gctgccatcg tggcggcgac ccgttccacc gccaagcctc cccgatgacc ccattgcaaa
     2401 acgatacgct gctgcgcgcg ctcaggcgcg agccgaccga ttacacgccg atctggctga
     2461 tgcggcaggc ggggcgttat cttcccgagt acaacgcgac gcgtgcgcgc gccggcagtt
     2521 tcatggcgct ttgcaccaat ccgcagcttg ccgccgaagt cacgatgcag ccgctcgcgc
     2581 gctttgcgct cgacgcggcg attctgtttt cggacattct caccgttccc gacgcgatgg
     2641 gtctcggatt gcatttcgtc gacggcgaag gtccgcgttt cgcgcggccg ctggtcgacg
     2701 aggaagcggt ggggaagctc gccgtgcccg atcccgagcg cctgcgttac gtatacgacg
     2761 ccgtcgcaac catcaagcgc gctctggccg gcaaactgcc gctgctcggg ttcgccggca
     2821 gcccgtttac gcgtgcctgt tacatgatcg aaggtggcgg cagcgcggac tttgcgcgcg
     2881 tgcgccgcat gctttatgcg cgccccgatc tgctgcatcg ggtcctctcg gtcaatgccg
     2941 aggcggtcgc cgcctatctg gcgcagcagg tcgcgcacgg cgccgacgcg ctgatgctgt
     3001 tcgacacctg gggcgggctg cttaccaccg aggcctatac acgtttttcg cttgcctaca
     3061 tccggacggt actcgaacgc gtgcggcagt cgaccggcgc cgtcacgccg acgatcgtgt
     3121 tcaccaaagg tggcggtcag tggcttgagc gcatcgccga caccggctgc tcggcgatcg
     3181 gcgtcgattg gacgaccgac atcgcggcgg ctcgcgcgag agtgggtgcg cgcgttgcgc
     3241 tgcaaggcaa cctcgatccg ctggcgctgc ttgccacacc ggacgtcgtc cgcgcggagg
     3301 ccgcggctgt cctggatgcg gcgactacct cgccgggctt
//
LOCUS       DATANS010000172         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_344841, whole genome shotgun sequence.
ACCESSION   DATANS010000172 DATANS010000000
VERSION     DATANS010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344841"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VHT22_02475"
     CDS             <1..598
                     /locus_tag="VHT22_02475"
                     /inference="COORDINATES: protein motif:HMM:NF015446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HEX3136446.1"
                     /translation="AVLGCDGIAAPSANRFGRISATTARHVAEEFGDAIALILDGGPS
                     RHGIESTIVDFSSGDPVVLRPGSITSGELARILGAAPRSAAAGATRAPGTLASHYAPR
                     TPAKLLPRSELLAALAGLGGSNAHISVLAHSVAQPPEFEGAWFDAPGESARYAQQLYA
                     NLRALDAREADEIWIEAPPDGPDWVAIGDRLRRATHRD"
     gene            753..1898
                     /locus_tag="VHT22_02480"
     CDS             753..1898
                     /locus_tag="VHT22_02480"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX3136447.1"
                     /translation="MPRRSPAVVLAPDSFKGSLSASDAAAAIAEGLRRVWPDADLRLC
                     PMADGGEGTLDAVLSRGGQRLTAGISDVAGKPLAVAYGLIGEPATAVVEVAQVVGLTD
                     PAVAAIDVEHRTTLGVGEMIRNRLDAGVRSFMIGLGGSSTNDGGAGLLAALGLSLTDA
                     RGRAIEPTPAGLATLAAVDASRLDPRLADARIIVMSDVNNPLTGERGATAIFGPQKGV
                     LRERIAEIDGHIANFASLTEGALDRRAQDKPGAGAAGGLGFALQLIGGEMRSGAEVVA
                     DLIGLDAALDGADWAITGEGKSDEQTALGKTPLIVARRAAARLIPATLISGSIDRSAL
                     PELGRHFAGCFSLPSGPASLEQCLEDAAALLADRAEQLALLFDAARG"
     gene            complement(1921..>2146)
                     /locus_tag="VHT22_02485"
     CDS             complement(1921..>2146)
                     /locus_tag="VHT22_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX3136448.1"
                     /translation="FGVILTINMEIGLIHPPVGLNIYIVSAIAPDVPVTRIMWGTIPY
                     VLCMMLQIVLLCIFPEIATWLPDHLMGAVH"
BASE COUNT          338 a          732 c          738 g          338 t
ORIGIN      
        1 cgctgtgctc ggctgcgacg gcatcgccgc gccctcggcg aaccgcttcg gacggatcag
       61 cgcaacgacc gccaggcatg tcgccgagga attcggcgac gcgatcgcgc tgatcctcga
      121 cggcggccca tcgcgtcacg gcatcgaatc gacgatcgtc gatttcagca gcggcgatcc
      181 tgtggtgctg cgtcccggat cgattaccag cggcgagctc gcgcgcattc tcggcgccgc
      241 gccgcgctca gccgcggccg gcgcgactcg cgccccgggg acgctcgcgt cgcactacgc
      301 gccgcgcacg ccggcaaagc tgctgccgcg cagcgagctg ctggcggcgc tcgccgggct
      361 cggtggatcg aacgcgcata tttcggtgct cgcccattcg gtcgcgcaac cgccggaatt
      421 cgagggcgca tggttcgatg cgccgggcga gagcgcgcgt tacgcgcagc agctctacgc
      481 caatttgcgc gcactggacg cgcgcgaggc cgacgagatc tggatcgaag caccgcccga
      541 cggacccgac tgggtcgcga tcggcgaccg tctgaggcgg gcgacgcatc gcgattagag
      601 cgtggccggc gttagcagcg cgcgtcgaac agcactccgc aggcggatag cgcgatggcg
      661 aagcgcgatg tcgcgcggat cgccgtgatc atgttcccga tcgcctgcta ccattgcatc
      721 gcaaccgcat cgatcgatca tacccgtaac ccatgccgcg acgttcgccc gccgttgtgc
      781 tcgctccgga ttcgttcaag ggatcgttgt cggcctcgga cgccgcggcg gcaatcgccg
      841 aaggcctgcg ccgcgtctgg ccagacgccg accttcgtct ttgtccgatg gccgatggcg
      901 gcgaaggcac gctggacgcc gtactctcgc gcggtggaca gcgattgacc gccggcatca
      961 gcgatgtcgc cggcaagccg ctggccgtcg cttatggatt gatcggcgag ccggcgactg
     1021 ccgtggtcga agtcgcgcaa gtcgttgggt tgaccgatcc tgccgtggcc gccatcgacg
     1081 tcgagcatcg cacgaccctg ggcgtcggcg aaatgatacg caatcgtctc gatgccggcg
     1141 ttcgcagctt catgataggc ctgggtggca gcagcaccaa tgacgggggc gccgggttgc
     1201 tcgcggcgct cggactctcc ctcaccgacg cgcgcggtcg tgcgatcgag ccgacgcccg
     1261 ccggcctcgc cacgctcgcg gccgtcgatg cgagccgcct cgacccgcgg cttgccgacg
     1321 cccggatcat cgtgatgtcg gacgtcaata atccgctgac cggcgagcgg ggcgcgactg
     1381 cgatcttcgg cccgcagaaa ggcgtgctgc gcgaacgcat cgccgagatc gacggccaca
     1441 tcgcgaactt cgcttcgctg accgagggcg cgctagatcg gcgcgcgcag gacaagcccg
     1501 gcgccggcgc cgccggcggc ctgggctttg cgctgcagtt gatcggcggc gagatgcgat
     1561 cgggcgcgga ggtcgtcgcc gacctgatcg gactcgacgc cgcgctcgac ggcgccgact
     1621 gggccattac cggcgaaggc aagagcgacg agcagactgc gctgggcaag acgccgctga
     1681 tcgtggccag gcgcgcggcg gcgcgcctga ttccggcgac cttgatctcg ggaagcatcg
     1741 accggtcggc tttgcccgag ctgggccgcc atttcgccgg ctgtttttcg ctgccgtccg
     1801 gcccggcgag cctcgagcaa tgcctggaag acgccgctgc gctgcttgcc gatcgcgccg
     1861 aacagctcgc gctgctgttc gatgccgcgc gcggctagga gggactaatg cacggcggta
     1921 tcagtgcacg gcgcccatca ggtggtcggg cagccaggtg gcgatttccg ggaaaatgca
     1981 cagcaacacg atctgcagca tcatgcaaag cacatacggg atcgtgcccc acatgatgcg
     2041 agtcaccggc acgtccggcg cgatcgcgct gacgatatag atgttgagcc cgaccggcgg
     2101 atgaatcagc ccgatctcca tattgatcgt caggatgacg ccgaac
//
LOCUS       DATANS010000173         1553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_51482, whole genome shotgun sequence.
ACCESSION   DATANS010000173 DATANS010000000
VERSION     DATANS010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51482"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..743)
                     /locus_tag="VHT22_02490"
     CDS             complement(45..743)
                     /locus_tag="VHT22_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX3136449.1"
                     /translation="MIELRKSTERGHADHGWLDSHHSFSFADYYDPRHMGFASLRVIN
                     EDVVQPGQGFGTHGHRDMEIITYILAGALEHKDSMGNGSIIRPGDVQRMSAGTGVRHS
                     EFNPSQEELVHLLQIWIEPAVTGIAPEYEEKHFDAASKRGRLRLIGSSDGRDGSVTIH
                     QDAAVYAALLDGAEHATYALAPGRKAYVHVARGAVSVNGRALNAGDAVKLSGEPAVEL
                     THGTAAEVLLFDLA"
     gene            complement(754..>1553)
                     /locus_tag="VHT22_02495"
     CDS             complement(754..>1553)
                     /locus_tag="VHT22_02495"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX3136450.1"
                     /translation="LGQPIVVDNRPGAGGNIGAELVAKSPADGYTMFMGTSGPLAINP
                     SLYKSIGFDPLRDFAPVILAASAPFVIVAHPSLAANNVKELIALAKAKPGSVNYGSVQ
                     GNASHLATELFDYMAGIKMTLVPYKGAAQATTDVIAGQIQVSFASTPGSVSLLKAGKL
                     KALAVTSAKRIAALPDVPTVAESGLPGYEASVWYGVVVPAKTPKEIVAKLNAEIGKIL
                     RERANRDKMAAADFEVTSSTPAEFGEFIRAETAKWTKVVKASGARAD"
BASE COUNT          247 a          512 c          489 g          305 t
ORIGIN      
        1 ctccggtgca aatgtcactt ggcgttccct ttgggtttag cgcatcaggc cagatcgaag
       61 agcaggactt ccgcggcggt cccgtgggtc agctcaaccg ccggctcgcc gctgagcttg
      121 accgcgtcgc cggcgttcag cgcgcggccg ttgacggaca cggcgccacg cgcgacgtgc
      181 acgtacgctt tacgccccgg cgcgagcgca tacgtcgcat gttcggcacc gtcgagcaaa
      241 gccgcgtaga ccgcggcatc ctgatgaatg gtcacagaac cgtcgcgccc gtcgctcgag
      301 ccgatcaggc gcaggcgccc gcgtttcgac gccgcgtcga aatgcttttc ttcgtattcg
      361 ggcgcgatgc cagtgaccgc cggctcgatc cagatttgca gcaggtgcac gagctcttcc
      421 tgcgaaggat tgaactcgct gtgccgcacg ccggtgcccg cgctcatgcg ctgcacgtcg
      481 ccgggccgga tgatggagcc gttgcccatg ctgtctttgt gctcgagcgc accggccagt
      541 atgtaggtga tgatttccat gtcgcggtgg ccgtgcgtgc cgaaaccctg gccgggctga
      601 acgacgtctt cgttgatcac gcgcaaactc gcgaatccca tgtgccgcgg gtcgtaataa
      661 tccgcaaacg aaaagctatg atggctgtcg agccagccgt gatcggcgtg gccgcgctcg
      721 gtgctcttgc gcaattcgat catggcgtcc tgctcagtca gcgcgcgcgc cagaggcttt
      781 gacgactttg gtccacttcg cggtctccgc gcggatgaat tcgccgaatt cggcgggcgt
      841 gctcgacgtc acctcgaaat cggccgccgc catcttgtcg cggttggcgc gctcgcgcag
      901 gattttgccg atttccgcgt tgagtttagc cacgatctcc ttcggcgttt tcgccggcac
      961 cacgacgccg taccagacgc ttgcttcgta gccgggcagg cccgactccg cgacggtcgg
     1021 cacgtcgggc agcgcggcta tgcgtttcgc gctcgtcacc gcgagggctt tcagcttacc
     1081 cgccttcaac agcgacaccg agcccggcgt cgacgcgaag ctcacctgga tctgacccgc
     1141 aatcacgtcg gtggtcgcct gcgccgcgcc cttgtacgga acgagcgtca tcttgatgcc
     1201 cgccatgtag tcgaagagct cggtcgccaa atgcgaggcg ttgccctgca ccgatccata
     1261 attgacgctg cccggtttgg ctttcgccag cgcaatcagt tccttcacat tgttcgcggc
     1321 cagcgacgga tgcgcaacga tcacgaacgg cgccgacgct gcaagtatga cgggcgcaaa
     1381 atcgcgcagc ggatcgaacc cgatgctttt gtacaggctc gggttgatgg cgagcggacc
     1441 cgaagtcccc atgaacatcg tgtagccgtc ggccggcgat ttcgcgacca gctcggcgcc
     1501 tatgttgccg cctgcgccgg ggcggttgtc gacgacgatc ggctggccga ggc
//
LOCUS       DATANS010000174         2686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_178227, whole genome shotgun sequence.
ACCESSION   DATANS010000174 DATANS010000000
VERSION     DATANS010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178227"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VHT22_02500"
     CDS             complement(<1..523)
                     /locus_tag="VHT22_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006872263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3136451.1"
                     /translation="MSGPLAGKVALVTGASRGIGRAIATKLAAGGCDIAVNYYNSHGE
                     AEALCAALRATGRRAYAFQASVGLPESVDEMFAAIATHFDRIDIVVSNAASGVLKPAM
                     AMSLKHWRWCLETNAFALALLAQRAVPQMRDGGRIIAMSSLGASRAMPDYGFIGASKA
                     ALESLVRTLAQELG"
     gene            complement(718..1755)
                     /locus_tag="VHT22_02505"
     CDS             complement(718..1755)
                     /locus_tag="VHT22_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136452.1"
                     /translation="MIATALALALSGCATAPLERPAEDLRAVTPGSVLRSLTIDPAVE
                     ARILALDPNHVSDDDVRNALAKGPTPRIFAIHGGVYGVYLLMESFGRFVTGMGYPEPM
                     IRDAGDGAYSRSPYEDSAQQAGEIAWYYEHEGVRPMLIGHSQGGIQVVKILHELAGTF
                     GAERRVFNPLTGQFEERTTIIDPLTGRERPVVGVSVADASVVGTGGWALALPVHWKVV
                     PTIRSIPDTVDEFTGYRIELDLFAWDAPGLENVKTFHSDGKAIVRNVTLPAEYSHVFV
                     PDTAQLALSAPMRAWINAFDPDNIAGRAPLPDGNANNVMWAADIWHSIKKHWCLEAQR
                     LLRAPRPPPAR"
     gene            complement(1782..>2686)
                     /locus_tag="VHT22_02510"
     CDS             complement(1782..>2686)
                     /locus_tag="VHT22_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136453.1"
                     /translation="DSIVRLLQRLVEDYYRIVPTDAQQPRLDLYPPGYRHLFARRFFA
                     LARGLSSWRYLIASCRRSVRPPYPKDGDGYNGVVFARLAPAESARLAATARSWGTTQN
                     DIFLAIMLKTLSPQVVERRQAKRRRDLAVGSIVNVRRDFGPRAAGGFAPLLGSFRVAH
                     PVPDDMPLAELARFVCGETSRIKRRKLYLQTLLAIGLSGLQWRFLSNQERQAFHAKNY
                     AVWAGVTPLQLNALWPEAKSYPFRVGYVRAVSTGPLSPLVMALTTLGDVVEVALSFRR
                     AAIDRRRAEVLLSDILQHIRSIAP"
BASE COUNT          475 a          878 c          906 g          427 t
ORIGIN      
        1 gcccgagctc ctgagccagc gtgcgcacca gcgactccag cgcggctttc gacgcgccga
       61 tgaaaccgta gtcgggcatc gcgcgcgagg cgcccaggct cgacatcgcg atgatgcgtc
      121 cgccgtcgcg catttgcggc accgcgcgct gcgccagcag cgccagcgcg aaggcattgg
      181 tctccaggca ccagcgccag tgcttgagcg acatcgccat cgccggtttg agcacgccgc
      241 tggcagcgtt gctgacgacg atgtcgatgc ggtcgaaatg agtggcgatc gcggcgaaca
      301 tctcgtcgac gctttcgggc aggccgacac tggcctggaa cgcgtacgcc ctgcggccgg
      361 tggcgcgcag cgccgcgcaa agcgcctcgg cttcgccgtg gctgttgtag taatttaccg
      421 cgatatcgca gccgccggcg gcgagcttgg tcgcgatcgc gcggccgata ccgcgcgaag
      481 ctcccgtgac gagcgcaacc tttccggcaa gcgggcctga catcggcgtt ggcggttcta
      541 tgaggattcc gggatggaca cggccgggtg caggcggcgc ggatggatcg ccctacgcca
      601 agccccggga taatcaagaa ttatcgcctg cttcgctctg ccgagcaacc tcgccgcccg
      661 gacgaaccgc agacttgcag gggctggcgc gccgagggcc tcgccggtgc gatcgcccta
      721 ccgggccgga ggcggcctcg gcgcgcgcag caaacgctgc gcctcgaggc accagtgctt
      781 cttgatgctg tgccagatgt cggcggccca cattacgttg ttcgcgttgc cgtccggcag
      841 cggcgcgcgc cccgcgatgt tgtcgggatc gaacgcattg atccacgccc gcatcggcgc
      901 ggacagggcg agttgcgccg tatcggggac gaagacgtgc gaatactcgg ccggcagcgt
      961 gacattgcgc acgatcgcct tgccgtcgga atgaaaagtc tttacgtttt ccaggcccgg
     1021 cgcatcccag gcaaagagat cgagctcgat ccgatagccg gtgaactcgt cgacggtgtc
     1081 gggaatcgat cggatagtgg gcaccacttt ccagtgcacc ggcaacgcaa gcgcccagcc
     1141 tccggtaccc accaccgacg cgtcggcgac cgatacgccg acgaccggcc gttcgcgacc
     1201 ggtcagcggg tcgatgatgg tcgtgcgttc ctcgaactgt ccggtcaggg gattgaacac
     1261 gcgccgctcc gcgccgaagg ttccggcgag ctcgtgcagg attttgacca cctggatgcc
     1321 gccctgactg tggccgatca gcatcgggcg cacgccttcg tgctcgtagt accaggcgat
     1381 ttctcccgcc tgttgtgcgc tgtcttcgta ggggctgcgc gagtaggcgc cgtcgccggc
     1441 gtcgcggatc atgggctccg gatagcccat gccggtgaca aagcgcccaa aggattccat
     1501 cagcagatag accccataga cgccgccgtg gatggcgaag atgcgcggcg tcggcccctt
     1561 ggcgagcgca ttgcgcacat cgtcgtcgct gacgtggttc ggatcgagcg cgagaattct
     1621 tgcttcgact gcgggatcga tcgtcaacga gcgcagcacg ctgcctggcg tgacggcgcg
     1681 cagatcctcg gccgggcgct cgagcggggc cgtcgcgcat ccggacaagg ccagcgccaa
     1741 agcggttgcg atcaagagtc ctgcccttcg gcacggcgtc atcatggcgc aatgctcctg
     1801 atgtgctgga ggatatcgct cagcaagacc tcggcgcggc ggcgatcgat tgccgccctg
     1861 cggaatgata aagcgacctc aacgacatcg cccagcgttg tcagcgccat gaccagcggc
     1921 gaaagcgggc cggtcgacac ggcacggacg tagcctacgc gaaacggata ggatttcgcc
     1981 tccggccaca aggcattgag ctgcagcggg gtgacccccg cccataccgc atagtttttg
     2041 gcgtgaaagg cctgccgctc ctggttcgaa agaaagcgcc attgcagccc ggacaagccg
     2101 atcgccagca aggtctgcaa gtagagcttg cgccgcttga tgcggctggt ttcaccgcaa
     2161 acgaaccggg cgagctccgc gagcggcatg tcgtcaggaa ccgggtgtgc aacgcgaaac
     2221 gatccgagca gcggcgcgaa gccgccggcg gcccggggtc caaaatcacg ccgcacgttg
     2281 acgatcgatc cgacggccag gtcgcggcgc cgcttggcct ggcggcgctc caccacctga
     2341 ggcgacaaag tcttgagcat gatcgcgaga aaaatatcgt tctgcgtcgt tccccaggat
     2401 cgcgccgtgg ccgcgagccg cgcggactcc gcgggggcga gccgggcgaa gacgacgccg
     2461 ttgtatccgt cgccgtcctt cggatacggc ggccggacgg agcgacggca gctcgcaatc
     2521 agatagcgcc agctcgacaa gccacgcgcc agcgcaaaaa accgccgagc gaacaagtgg
     2581 cgatatccgg gcgggtacag gtcgaggcgc ggctgctggg cgtcggtggg cactatgcgg
     2641 taatagtcct cgaccagtcg ctgcagcagg cggacgatcg agtcgc
//
LOCUS       DATANS010000175         2532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_303779, whole genome shotgun sequence.
ACCESSION   DATANS010000175 DATANS010000000
VERSION     DATANS010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303779"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VHT22_02515"
     CDS             <1..874
                     /locus_tag="VHT22_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX3136454.1"
                     /translation="DAAAYIRSLAQLRGRNVEWAERAVRESVSLSADEALAQHVIDLT
                     ARDVPDLLTKLNGRKIVTAGGERILATSGAATTMVAPDWRTRFLAIITDPSVALMLLM
                     AGIYGLFFEFWNPGLALPGVLGAICLLLGLFALQMLPVNYAGLALVLLGIAFIVAETF
                     LPAYGSLGVGGIVAFAIGAVMLIDTDVPGFGVPYWLIATLAAVTALFVFFVAGAALKA
                     RRRPIVTGHEQLIGSIGVALDDLETEGWARIHSEQWRVKSPVPIKRGQRVRVTARSDL
                     LLSVLPVTEAEQGA"
     gene            877..1644
                     /locus_tag="VHT22_02520"
     CDS             877..1644
                     /locus_tag="VHT22_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX3136455.1"
                     /translation="MAIGVGFGTIVIIVILVVMMSFRVLREYQRAVVFMLGRFWKVKG
                     PGFFLLIPVIQQMVRIDLRTVVMDVPPQDVITRDNVSVKVNAVVYFRILDPAKAVIQV
                     ENYYQATSQLAQTTLRAVLGKHELDELLSAREKLNLDIQQVLDNQTEAWGIKVSTVEM
                     KDVDLNETMIRAIARQAEAERERRAKIIHAEGELQASEKLLQAAQMLSKQPQAMQLRY
                     LQTLANIAGDKSSTIVFPLPIDLLSAMASKRTESPSP"
     gene            1881..2069
                     /locus_tag="VHT22_02525"
     CDS             1881..2069
                     /locus_tag="VHT22_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136456.1"
                     /translation="MMVSVWWLVWVFLTGAYSGFLLLALLRMGDDRDEGAHEALMAHR
                     ALSWRHGRERRGFVGPTP"
     gene            complement(2125..>2532)
                     /locus_tag="VHT22_02530"
     CDS             complement(2125..>2532)
                     /locus_tag="VHT22_02530"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX3136457.1"
                     /translation="IARLTGLPVLVTGGSTYGGTTEAELMQEALQHEFGVPVRWAENR
                     SRTTHENAQYSAALLRKAGIGRVVLVAHAFDVRRATAEFAAAGIATVPAATGIPSRGP
                     VRVLDYLPAINGLQGSYYALYEILGNAVRVVTP"
BASE COUNT          416 a          828 c          829 g          459 t
ORIGIN      
        1 cgatgcggcg gcgtatatcc gctcgctcgc gcagctgcgt ggtcgtaatg tcgaatgggc
       61 cgagcgcgcg gtgcgcgaat cggtcagcct gtcggccgac gaagcactgg cccagcatgt
      121 gatcgatctg accgcgcgcg acgtcccgga tctcttgacc aagctcaacg ggcgaaagat
      181 cgtcacggcc ggcggcgagc gcatcctggc gacatcgggt gcggcgacga cgatggtggc
      241 ccccgattgg cgcacgcgat tcctggcgat catcaccgat ccgagtgtgg cgctgatgct
      301 gttgatggcg gggatctacg gcctgttctt cgaattctgg aatcccgggc tggcgctgcc
      361 gggcgtgctc ggcgcgatct gcctgctgct cgggctgttc gcgctgcaga tgctgccggt
      421 caactatgcc ggtctcgcgc tggtgctgct cggcatcgcg ttcattgtcg ccgagacgtt
      481 cctgcccgcc tacggctcgc tcggtgtcgg ggggatcgtg gcattcgcca taggtgcagt
      541 catgctgatc gacaccgatg tacccggttt cggcgtgccg tactggctga tcgcgacgct
      601 ggccgccgtc accgcgctgt tcgtcttctt cgtcgccggc gccgcactca aggcgcggcg
      661 acgcccgatc gtgaccggtc acgagcagct gatcggcagc atcggcgtcg cgctggacga
      721 tctggagacc gagggctggg cacgcatcca cagcgagcag tggcgggtca aaagcccggt
      781 gccgatcaag cgtggccagc gcgtgcgcgt gacggcgcgc agcgatctgt tattgtcggt
      841 attgcccgtc acggaagccg aacaaggagc gtgagcatgg ccatcggagt gggattcggc
      901 acgatcgtca tcatcgtcat tctggtggtg atgatgtcgt tccgcgtgct gcgcgaatac
      961 cagcgcgcgg tcgtattcat gctcggccga ttctggaagg tcaaggggcc cggcttcttc
     1021 ctgctgattc ccgtcatcca gcagatggtc cggatcgacc tgcgcacggt ggtgatggac
     1081 gtgccgccgc aggatgtgat cacccgcgac aatgtctcgg tcaaggtcaa cgcggttgtg
     1141 tactttcgca tcctagatcc ggccaaggcg gtgatccagg tcgagaacta ttaccaggcg
     1201 accagccagc tggcgcagac gaccttgcgc gcggtgctcg gcaagcacga actcgatgag
     1261 ctcttgtccg cacgcgaaaa gctcaacctc gacatccagc aggtgctcga caaccagacc
     1321 gaggcctggg gcatcaaggt atcaacggtc gaaatgaagg acgtcgacct gaacgagacg
     1381 atgatacgtg cgatcgcgcg tcaggccgag gccgagcgcg agcggcgcgc caagatcatc
     1441 cacgccgaag gcgaattgca ggcgtcggaa aaattgctgc aggcggcgca gatgctctcg
     1501 aagcagccgc aggcgatgca gcttcgctat ttgcagacgt tggccaacat cgccggcgac
     1561 aagagctcga cgatcgtttt cccgctgccg atcgacctgt tgagtgcgat ggcgagcaag
     1621 cggaccgagt ccccgtctcc ctaaagcccc gccggctggc ttccgccttc ccacgcgctt
     1681 ccggcgccgc cgcgccccgc gccctgcggc atttgccgcc ccgggccgat gccaccgcgc
     1741 tcatccgccg gctactgaat catcccgaat caagacctta gaccaatggc acatcagttg
     1801 cttgcctttg tcccggggcg ggcccggcct caggcaccaa ctccgggacc gcggcaaatg
     1861 atcaaaagtg gggagttcct atgatggttt ccgtgtggtg gctggtctgg gtatttctga
     1921 cgggagcgta ttccggcttc ctgctgctgg cgttgctgcg tatgggcgac gaccgcgacg
     1981 aaggcgcgca cgaagcgctg atggcgcacc gcgccctgtc gtggcgtcac ggccgcgagc
     2041 gccgcggctt tgtcggaccc acgccgtaac acgcgcgacg gcggcgcctt tcgccgccgg
     2101 ccgcaaggct tgcccttcga cgcctcatgg tgtgaccacc ctcacggcgt tgcccaggat
     2161 ttcgtacaac gcgtaataac ttccctgcag cccgttgatg gcgggcaggt aatcgagcac
     2221 ccgcaccggg ccgcgcgacg gtatgccagt tgccgcgggc accgtcgcga taccggccgc
     2281 cgcaaactcg gccgtggcgc ggcgcacatc gaaggcgtgc gcgaccagca ctacccgtcc
     2341 gatccccgcc ttgcgcaaca gtgccgccga gtattgcgcg ttctcgtgcg tcgtacgcga
     2401 gcggttctcc gcccagcgca ccggaacacc gaactcgtgc tgcaacgcct cttgcatcag
     2461 ctcggcctcg gtggtcccgc cgtaggtcga gccgccggtg acgagcaccg gcagaccggt
     2521 gagccgcgcg at
//
LOCUS       DATANS010000176         2359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_219162, whole genome shotgun sequence.
ACCESSION   DATANS010000176 DATANS010000000
VERSION     DATANS010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219162"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="VHT22_02535"
     CDS             complement(<1..172)
                     /locus_tag="VHT22_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX3136458.1"
                     /translation="MTAPLDGVRVLDLSRLLPGPVCTLHLADLGADVVKVEDTGSGDY
                     ARALGLAPTGDGR"
     gene            complement(169..1338)
                     /locus_tag="VHT22_02540"
     CDS             complement(169..1338)
                     /locus_tag="VHT22_02540"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX3136459.1"
                     /translation="MIAPGSWLGLLGGGQLGRMFCMAAQSMGYRVAVLDPGDESPAGS
                     VADRHILAHYGDRHGLATLADLVEAATTEFENVPAASLEYLGRRARVTPSADSVAIAQ
                     DRIREKQFLANNGFAVAPFAVLATEADARAIDPALLPGIVKSARFGYDGKGQIRIDDA
                     AGVPAAFAAMGAAPCVLERMVPLTCELSVIVARNDRGDAVTWPVAENRHRDGILDSTI
                     VPARIAPALASEARDVALRVAAALDYRGVLCVEFFVVDGVLLINEIAPRPHNSGHYTI
                     DACVTSQFEQQVRILADLPLGDTRAHGPAVMVNLLGDVWFDTPSGTPDAARREPDWRA
                     VLEVPEAKLHLYGKDEARRGRKMGHVTCVAPTLDQALAAARAIRRQLRIPDDGAL"
     gene            complement(1335..1850)
                     /gene="purE"
                     /locus_tag="VHT22_02545"
     CDS             complement(1335..1850)
                     /gene="purE"
                     /locus_tag="VHT22_02545"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009565304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX3136460.1"
                     /translation="MARQQKSVQVGVVMGSQSDWDVMQHAVAQLAAFGVSCEAKVVSA
                     HRTPDAMFAYASGAAARGLSCIIAGAGGAAHLPGMLAAKTMLPVLGVPIPTRHMNGAD
                     SLLSIVQMPKGVPVATFAIGEAGAANAALFAVAMLARGNAALAKKLADFRAAQTTATG
                     AMTLPPKLRAK"
     gene            1985..>2359
                     /locus_tag="VHT22_02550"
     CDS             1985..>2359
                     /locus_tag="VHT22_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136461.1"
                     /translation="MLTSPTRGTALRCLAFIAAGVFAVALRAEPTDADFLAARAAFLA
                     GDAATLDRIAPRANGHMLEPYVAYWQLRLKLDEATPERVRSFLERNDGTPLADTLRGE
                     WLKALGKRALWSEFAAEYPKRNG"
BASE COUNT          375 a          801 c          811 g          372 t
ORIGIN      
        1 cgcggccgtc gccggtcgga gcgagaccaa gcgcgcgtgc gtaatcgccg gacccggtgt
       61 cctcgacctt gacgacatcc gcgccgaggt cagccaggtg cagcgtgcaa accggcccgg
      121 gcagcagtcg ggaaagatcg agcacgcgca caccgtcgag cggcgcggtc acagtgcccc
      181 gtcgtcggga attcgaagct gccgccggat cgcgcgtgcc gcggcgagcg cctgatcgag
      241 cgtcggcgca acgcaggtca catgacccat cttccgcccg cggcgcgctt cgtccttgcc
      301 atacaggtgc agcttcgcct cggggacttc cagcaccgcg cgccaatcgg gctcgcggcg
      361 ggcagcgtcc ggcgtgccgg aaggtgtgtc gaaccagacg tcgccgagca ggttgaccat
      421 gaccgccggg ccgtgcgccc gggtatcgcc gagcggcagg tcggccagaa tgcgcacctg
      481 ctgctcgaat tgcgaggtga cgcaagcatc gatcgtgtag tgcccgctgt tgtgcggcct
      541 cggcgcgatt tcgttgatca acaggacgcc atcgacgaca aaaaactcga cacacaagac
      601 gccgcgataa tcgagcgcgg cggctaccct gagtgctacg tcccgggctt cggacgcgag
      661 ggcgggggcg atgcgcgcag gcacgatcgt cgagtccagg atgccgtcgc gatggcgatt
      721 ttcggcgacc ggccaggtca ccgcgtcgcc gcggtcgttc cgcgcgacga ttaccgacag
      781 ctcgcaggtc agcgggacca tgcgctcgag gacgcaaggg gcggcaccca tcgcggcgaa
      841 cgccgccggg acccccgccg cgtcgtcgat ccggatctgg cctttgccgt cgtagccgaa
      901 gcgcgcgctc ttgacgatgc cgggcagaag tgccggatcg atcgctcgcg cgtcggcctc
      961 ggtcgccagc acggcgaacg gcgccactgc gaagccgttg ttcgcgagga actgcttttc
     1021 gcggatgcgg tcttgcgcaa tggcgacgct atccgccgac ggcgtgacac gggcgcggcg
     1081 tccgagatat tcgagactcg cggccggaac gttttcgaac tcggtcgtgg ccgcttcgac
     1141 cagatcggca agggtagcca ggccgtgtcg gtcgccatag tgggcaagga tgtgccgatc
     1201 ggcgaccgat cccgcagggc tctcgtcgcc gggatcaagc acggcgacgc gatagcccat
     1261 gctttgcgcg gccatgcaga acatcctgcc cagctgtcct ccaccgagca acccgagcca
     1321 tgagcccggg gcgatcactt cgcgcggagc ttcggcggca gcgtcatcgc acctgtggcg
     1381 gtggtttgcg cggcgcggaa atcggcgagc tttttggcaa gcgcggcgtt gccgcgcgcg
     1441 agcatcgcga cggcgaagag tgccgcgttc gccgcgccgg cttcgccgat ggcgaacgtc
     1501 gccaccggca ctcccttggg catctgcacg atggagagca gagaatccgc gccgttcata
     1561 tgtcgcgtgg gtatcgggac gcctagcaca ggcagcatcg tcttggcggc gagcattccc
     1621 ggcaggtgcg cagcgccccc ggcgccggcg atgatgcacg agagcccgcg tgccgccgcg
     1681 ccgctggcat aggcgaacat cgcgtcgggc gtgcggtgag cggatacgac cttggcttcg
     1741 cagctgacgc cgaaagcggc aagctgcgca acggcatgct gcatcacgtc ccaatcgctt
     1801 tggctgccca tgaccacgcc gacctgaacg gacttttgct gtcgagccat ctgttgtcct
     1861 atcgcttcga ggcgggccgc tgccggcagg cgatgatttt accatcggcc gcggcgcgcc
     1921 ttgccgacgc ggcgtcgggc atccgatcgt cgacgaagct cgacaatagg cgataatcgc
     1981 gctgatgctc acgtcgccta ctcggggaac ggctctgcgc tgtctggcgt tcatcgccgc
     2041 cggcgtcttc gctgtcgctc tgcgcgccga gccgaccgac gccgattttc ttgcggcgcg
     2101 cgcggcgttt ctggccggcg acgccgcgac cctggacagg atcgcgccgc gggccaacgg
     2161 ccatatgctc gagccctacg tcgcctactg gcagttgcgc ctcaagctcg acgaggcgac
     2221 gcccgagcgg gtccgctcgt ttctcgagcg caatgacggc acaccgctgg ccgacaccct
     2281 gcgcggcgaa tggttgaagg cgcttggcaa gcgcgcgctc tggagcgagt tcgccgccga
     2341 atacccaaag cgcaacggc
//
LOCUS       DATANS010000177         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_430359, whole genome shotgun sequence.
ACCESSION   DATANS010000177 DATANS010000000
VERSION     DATANS010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430359"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VHT22_02555"
     CDS             <1..308
                     /locus_tag="VHT22_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990531 - phospholipid-translocating
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005548 - phospholipid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEX3136462.1"
                     /translation="SPVKAAGVVVGRVESIKLDPKTYAAVVTMRIDRDYAFSKDTIAS
                     INTSGLLGEVYIGLDAGGDTAMLNDGDRIAKTQSAVVIEKLISQFLFDKAAQGGAGK"
     gene            305..1012
                     /locus_tag="VHT22_02560"
     CDS             305..1012
                     /locus_tag="VHT22_02560"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEX3136463.1"
                     /translation="MNSFSRRIAWTLALACVLLAGCAGTPSRVDPFEPWNRAMYSVHE
                     VVDGNVIKPIAQTYVKVTPEPVRTGVSNFYGNIDDLFTGINHVLQGKKERAGDDFGRV
                     LLNSTFGMLGIFDLASEMGITKDKTDFGITFGKWGAPQGPYLFVPLFGPTTVRDGTGW
                     IVRLYTGPIGYIDNVPLRNWLIALGYLDTRAQLLSAESVLDAAALDKYRYLRNAYLRA
                     RRYQVYDGKPPPEEEEE"
     gene            1092..1697
                     /locus_tag="VHT22_02565"
     CDS             1092..1697
                     /locus_tag="VHT22_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3136464.1"
                     /translation="MIYRWVAILFAASLALSAQAQEAPDVMVKRVAEDVLQTIRSDKD
                     LQAGNQAKVKQLIESKLAPNFDFTRMTALAMGKNWRSATPEQQKVLTDEFRTLLVRTY
                     SGALSNYRDNTMDYAPLRSNPGDTEVIVRTSVRRPGQAPVQIDYSMAKTPDGWKAYDV
                     IVAGVSLVTNYRDEFNDAIKSSGVDGLIKMLGDKNKGPTPK"
     gene            1694..2023
                     /locus_tag="VHT22_02570"
     CDS             1694..2023
                     /locus_tag="VHT22_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX3136465.1"
                     /translation="MTKRAQPAVPVVPVAQVLRADDGRWVLAGPLTIDNVAGVLAESV
                     GVPLPTTGRVDLKGVDPVDSAGVAVLLEWKRRAVAEGVDLAFENVPPTMASLAELYGV
                     DGLLSPA"
     gene            2139..>2266
                     /locus_tag="VHT22_02575"
     CDS             2139..>2266
                     /locus_tag="VHT22_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3136466.1"
                     /translation="MSAAVEIVDVARRFGSVQALAGIDLAVEEGEFFGLLGPNGAG"
BASE COUNT          420 a          688 c          757 g          401 t
ORIGIN      
        1 gctcgccggt gaaggccgcc ggcgtcgtcg tcggccgagt cgagtcgatc aagctcgacc
       61 caaagacgta tgcggcggtt gtcacgatgc gcattgatcg cgactatgct ttcagcaagg
      121 atacgatcgc ctcgatcaac acctcggggt tgctcggtga agtctacata ggtctcgatg
      181 cggggggcga cacggcgatg ctgaacgacg gcgatcgcat cgccaagacg caatcggcgg
      241 tggtcatcga gaagctgatc agtcagttcc tgttcgacaa ggcggcacaa ggaggcgccg
      301 gcaaatgaat tctttcagtc gccgcatcgc ttggacgctc gctctggcct gtgtgctgct
      361 cgccgggtgc gccggaacgc cgtcgcgagt cgaccccttc gagccgtgga accgtgcgat
      421 gtactcggtg catgaggtgg tcgacggcaa cgtgatcaag cccatcgccc agacctacgt
      481 caaggtgacg ccggagccgg tgcggacagg tgtgtccaac ttttacggca acatcgacga
      541 tttgttcacc ggcatcaacc acgtgctgca gggaaagaag gagcgcgccg gcgatgattt
      601 cggacgcgtg ctgctcaatt cgaccttcgg tatgctcggc attttcgacc tcgcgtccga
      661 gatgggcatc accaaggaca agaccgactt cggcatcacg ttcggcaagt ggggcgcgcc
      721 gcagggcccg tatcttttcg taccgctgtt cggaccaacc accgttcggg acggaaccgg
      781 ctggatagtg cgcctttaca cgggaccgat cggctatatc gacaacgttc ccttgcgcaa
      841 ttggctcatc gccctcggct atcttgacac ccgggcgcag ctgctgagcg ccgagagcgt
      901 gctcgatgcc gcggcgctcg acaaatatcg ttatctgcgg aacgcctatt tgcgggcgcg
      961 gcgttatcag gtctacgacg gcaagccgcc gccggaagag gaagaggaat gaacggggaa
     1021 gcacggccgt ccgcgatcgc gtgcggcgcg ccggccggct ttcagggcat cgatattcga
     1081 cgaggttcac gatgatttac cgatgggtag caatactgtt cgcggcctcg ctggcgctga
     1141 gtgcgcaggc gcaggaagcg cccgatgtta tggtcaagcg cgttgccgag gacgtgctgc
     1201 agacgatacg cagcgacaag gatctgcagg ccggcaacca ggcgaaggtc aagcaactga
     1261 tcgagagcaa gctggcgccg aatttcgact tcactcgcat gaccgcgctg gcgatgggaa
     1321 agaactggcg cagcgccacg cccgagcagc agaaagtcct caccgatgaa tttcggacgc
     1381 tgctggtgcg gacctattcc ggcgcgctgt ccaactatcg cgacaacacg atggactacg
     1441 cgccgctgcg gtcgaatcct ggcgacaccg aggtgatcgt gcgcacgtcg gtgcgacggc
     1501 ccggtcaggc gccggtccag atcgactaca gcatggcgaa gactccggat ggctggaagg
     1561 cgtacgacgt gatcgtcgcc ggcgtgagcc tggtgaccaa ctatcgcgat gaattcaacg
     1621 atgcaatcaa gagctctggc gtcgacggcc tgatcaagat gctgggcgac aagaacaagg
     1681 ggccgacgcc gaagtgacca agagggcgca gccggcagtg ccggtcgtac cggtcgcgca
     1741 ggtgctgcgc gccgacgacg gccgctgggt gctcgccggt ccgctgacga tcgacaacgt
     1801 cgccggtgtg ctcgcggaaa gtgtcggcgt accgctgccg acgaccggtc gagtcgatct
     1861 caaaggcgtc gatccggtcg attcggccgg cgtggccgta ttgctggaat ggaagcgccg
     1921 ggccgtcgcc gaaggcgtgg atctggcgtt cgagaacgtc ccgccgacca tggcctcgct
     1981 ggccgagctc tacggcgtcg acggactgct ttctcccgcc tgaacatctt ggtccggatt
     2041 ttttcgcgat tacgcgccct gtcgcgggcg cgtatcctcg atcgcctcga atcggcgctt
     2101 ccgcggcgcg cggcgccagc cgcgagtgtg aggggccgat gagcgctgcg gtcgaaatcg
     2161 tcgacgtcgc gcggcgcttc ggatccgtgc aggcgctcgc cggcatcgac ctcgcggtcg
     2221 aagagggcga gttcttcggc ctgctcggcc ccaacggggc cggcaa
//
LOCUS       DATANS010000178         3548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_93843, whole genome shotgun sequence.
ACCESSION   DATANS010000178 DATANS010000000
VERSION     DATANS010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93843"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VHT22_02580"
     CDS             <1..242
                     /locus_tag="VHT22_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEX3136467.1"
                     /translation="TLPGAIGTTPALAALSSIAGDPAALLDRLDAQLLHSTMPPSMRG
                     AILTALAAVPATDLLTRAKTAVYLVVTSPQYQVER"
     gene            244..1620
                     /locus_tag="VHT22_02585"
     CDS             244..1620
                     /locus_tag="VHT22_02585"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3136468.1"
                     /translation="MKAIAIPRRAFLRQLGALSALGLASRLDLIRFAAEASAQTQPDY
                     KALVCVFLFGGNDGNNTVIPLDAAGYGQYAAIRPATSGINLSQASLLPIQPIDSASPF
                     GLHPSLTDLQMLFNQNRMAILANVGTLLQPTTQAQYAAGLRPLSLYSHADQQAQWQSA
                     VSSAASGTGWGGRIADKVAPMNAASGFPVVTSLDGAVLFTSGASAIPLSIPVTGSFAL
                     SGYSGSTAAAARMNAVQQLLAQASGNALVDGASDIGAQALDLSATVNPILANANSTIA
                     PLFTNLNTSTANQLYQVAKLIEARAATGAARQIFFVQLGSFDTHGDQLNRQQNLFEEL
                     SPALKAFYDATVALGVASQVTTFTLSDFGRTLQPASGGGTDHAWGSHHFIIGGAVQGG
                     KIYGRYPQLSLGGPDDAEKEGRWLPSCSVDQYGATLARWFGVATTDLDSVFSNLASFS
                     TADLGFMG"
     gene            1843..>3548
                     /locus_tag="VHT22_02590"
     CDS             1843..>3548
                     /locus_tag="VHT22_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX3136469.1"
                     /translation="MVTVGVAMALRCAPAAADVAVEAYRVEVVAPAPVKAAVERSLDL
                     VRWQSYAEITPEFFDLLVADARAQARDAAAAQGYFSAVVTSEIDRSTKPETVRLRIEP
                     GQPTTVASVELLVTGAAASGGPEGEKIAARMRQEWPLPKGAVFRQEDWAAGKTSAVRV
                     FTVERYAAAALVVSRAEVDPEAHTAALSLTVDSGPPFRFGALDVTGVQKYSEALVRNL
                     VTFSPGEPYTAEALQVFLRRLNGSGYFASAQATVESDPAQADAAPVHLRVIEAPEKKI
                     TAGAGFSTDTLFRAQLTYDDFNIDDAGLRFHTDLDVESKIQGVTMRLTLPPRVPQYTD
                     SYSTSVAHTDISGLRTNEFMAGWQRKTAVERDQVTYSAGFYASQQMPTNAESANAFAL
                     YFEYGRTWRNVDELLSPNRGYSINARVGAAPPGVSTAAMGRGILQFAAWLPVDTKTQI
                     VFKLEGGAVAAREARDVPIQLLFRTGGDTTVRGYEFNSLGPRIGDATVGGRYYALGSA
                     EVVRWIGDFWGIATFVDAGNAADQPRDLKPVYGYGAGVRLRTPIGPMRMDVAYGQETH
                     SVR"
BASE COUNT          600 a         1232 c         1117 g          599 t
ORIGIN      
        1 cgaccttgcc cggagcgatt ggcacgacac ccgcgctggc ggccctgtcc tcgatcgccg
       61 gggatccggc agcgctgctc gacaggcttg acgcgcaatt gctgcatagc acgatgcccc
      121 cgtcgatgcg cggcgcgatc ctgacggcgc tcgccgccgt tccggcgacg gacctgctga
      181 cgcgcgcgaa gaccgccgtc tatctggtcg tcacatcgcc gcaataccag gtggagcgct
      241 gaaatgaaag ccatcgccat accgcgccgc gcatttcttc ggcaactggg cgcgctgtcc
      301 gctttaggac tcgcttcccg cctggacctg atccgtttcg ccgccgaggc cagcgcacag
      361 acgcagccgg actacaaggc actggtctgc gtgttccttt tcggcggcaa cgacggcaac
      421 aacacggtca ttccgctcga tgcggcaggc tacggccagt atgccgctat tcgtccggcc
      481 acatccggga tcaacctgtc gcaggcatcg ctgctgccga tccaaccgat cgactccgcc
      541 tcgccgttcg ggctgcaccc gtcgctcacc gatctgcaga tgctgttcaa ccagaaccgc
      601 atggcgatcc ttgccaacgt cggcaccttg ctgcagccga cgacacaggc gcaatacgcg
      661 gccgggttgc gacccttgtc gctgtattcg cacgccgacc aacaggcgca atggcaaagc
      721 gcagtttcga gcgccgcgtc gggaaccggc tggggtggcc gcatcgccga caaggtcgcg
      781 ccgatgaacg cggcgtcggg ctttcccgtt gtcacctcgc tcgatggggc cgtgctgttc
      841 accagcggcg cgtcggccat tcccttgtcg atacccgtca ccggctcgtt cgccctgtcc
      901 ggctacagcg gcagcacagc agccgcagcg cgcatgaatg cggtgcagca gcttttggcg
      961 caagcgtcgg gcaacgcgct ggtcgacggc gcatccgaca tcggcgccca ggcgctcgat
     1021 ttgtcggcta ccgtcaatcc gattctcgcc aacgcaaact ctacgatcgc gcccctgttc
     1081 acgaacctga acacatctac cgccaatcaa ctctaccagg tcgccaaact gatcgaggcg
     1141 cgcgcggcga ccggcgcagc gcggcagata tttttcgtcc agctgggcag cttcgacact
     1201 catggcgacc agttgaaccg gcagcaaaac ctgttcgaag agctctcacc ggcgttaaag
     1261 gccttttacg atgcgaccgt cgcgctgggc gttgcttcgc aagtcacgac gttcacgctg
     1321 tcggacttcg gccgcacgct gcagcccgca tccggcggtg gtaccgacca cgcttggggg
     1381 agtcaccatt tcatcatcgg cggagcggta cagggcggaa aaatatacgg tcgctatccc
     1441 cagctctcgc taggcggccc ggacgacgcg gaaaaggagg gccgctggct gccttcctgc
     1501 tcggtcgatc agtacggcgc gaccttggcc cgctggttcg gcgtcgccac gaccgacctg
     1561 gacagcgtct tttccaatct ggccagcttt tcgaccgccg acctgggctt catgggctga
     1621 cacggttcgc aggctggcgc gcggcgctgg cttgacgttg ctgcccggct gccgctacgc
     1681 tcgacgcgtt ccccgcttcg ggttccaatg gcggcgttcg catgctcgag tcgccgccac
     1741 cgcatcgctt ccagacgagt gaatccagcg cgtcggctta gcattccgtt tccgcctcgc
     1801 gcgacgctcg tcaccgcgac gtgcgcgttt gctcgcgcga tcatggtcac cgtcggcgtg
     1861 gcgatggcat tgcgttgtgc gccggcggcc gccgatgtcg cggtggaagc gtaccgggtc
     1921 gaagtcgtcg cgccggcgcc ggtcaaggcc gccgtcgagc gcagcctcga tctggtgcgc
     1981 tggcagtcgt atgccgaaat cacgccggag tttttcgatc tcctggtcgc cgatgcaaga
     2041 gcacaggcgc gcgacgcggc ggcggcgcag ggttacttct ccgcggtggt cacaagcgaa
     2101 atcgaccgat cgaccaaacc cgagaccgtg cgcctgcgga tcgaaccggg ccagccgaca
     2161 accgtcgcgt cggtcgaatt gctggtcacc ggcgcggcag ccagcggcgg tcccgagggc
     2221 gagaaaatcg ccgcgcgcat gcgtcaggaa tggccgttgc ccaaaggcgc ggtattccgc
     2281 caggaagact gggccgccgg caaaaccagt gcggtgcgcg tgttcaccgt cgaacggtac
     2341 gctgcggcgg ccctggtcgt cagccgcgct gaggtcgatc cggaagcgca taccgccgcg
     2401 ctcagcctga ccgtcgacag cggcccgccg ttccgcttcg gcgcgctcga tgtgaccggc
     2461 gtgcagaaat acagcgaggc gctggtcagg aatctggtga ccttcagccc gggcgagccc
     2521 tacacggcgg aagcgctgca ggttttcctg cgccggctca acggctcggg ctatttcgcc
     2581 agcgcgcagg cgacagtcga gtccgacccc gcgcaagccg atgccgcgcc agtccatctt
     2641 cgcgtgatcg aggccccgga aaagaagatc accgccggcg caggcttttc gaccgacacg
     2701 ctgttccggg cccagttaac ttacgacgat ttcaacatcg acgatgccgg actgcgcttc
     2761 cacaccgacc tcgatgtcga gagcaagatc cagggcgtga ccatgcgcct gaccctgccg
     2821 ccgcgcgtgc cgcaatacac cgacagctac tcgaccagcg tcgcgcacac cgacatttcc
     2881 ggcctgcgga ccaacgagtt catggctggc tggcagcgca agacggcggt cgagcgcgac
     2941 caggtcacct acagcgcggg cttctacgcc tcgcaacaga tgccgaccaa tgcggaatcg
     3001 gccaatgcgt tcgcgcttta cttcgaatac ggccgcacct ggcgcaacgt cgacgaactg
     3061 ctgtcaccga accgcggcta ctcgatcaac gcgcgggtcg gcgctgcgcc gcccggtgtc
     3121 tcgaccgccg cgatgggccg cggcatcctg caattcgcgg catggctgcc ggtcgacacc
     3181 aagacacaga tcgtattcaa gctcgagggc ggcgccgtcg ccgcgcgcga ggcgcgcgac
     3241 gtaccgatcc agctgctctt ccgcaccggc ggcgatacga ccgtgcgcgg gtacgaattc
     3301 aacagccttg gaccgcgcat cggcgatgca acggtcggag gacgctacta cgcactcggc
     3361 agcgcggaag tcgtgcgctg gatcggcgat ttctggggca tcgcgacatt cgtcgacgcc
     3421 ggcaacgccg cggatcaacc ccgggatctg aaaccggttt acggctatgg cgccggcgtc
     3481 cgcctgcgca cgccgatcgg tccgatgcga atggacgtcg cctacggcca ggagacgcac
     3541 tcggtccg
//
LOCUS       DATANS010000179         1866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_135527, whole genome shotgun sequence.
ACCESSION   DATANS010000179 DATANS010000000
VERSION     DATANS010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135527"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VHT22_02595"
     CDS             complement(<1..1118)
                     /locus_tag="VHT22_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136470.1"
                     /translation="MLVGASLFAASTLLCIVGYLALSVPGSWFGSLPTLRWTAAELAV
                     TQGSGQPTADGLRVSAPGKQGIAIVSIQTSFRSAGYTAVAWDTADVPNDVAAALIWRN
                     DYEPGRRFTRPLTVEAKRLLPITLADDPNWIGKINGLAVIIRGDLTQPMMIRGVEAKP
                     MTAVQVLHDRIREWFAFESWSGGSINSRSGGADAQDLPLPFALAAIVGLALIIYVILA
                     RWRPQTIGPFRPLIAGGLFVCAWFILDVPWQWNLVREARATFQQYGGKSWRERHLAAE
                     DGALFAFIEKARAKLPPPPVRVFMGADSLYFRDRGAYHLYPYNVYFDPYRNTMPDAAK
                     LRGGDYLVVFRRKGVQFDPAQKKLRWDGGQALDAEILFA"
     gene            complement(1213..>1866)
                     /locus_tag="VHT22_02600"
     CDS             complement(1213..>1866)
                     /locus_tag="VHT22_02600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HEX3136471.1"
                     /translation="LLRHSFSEIAGMPVIGLSDTPIEGTARLLKAGEDYALGALLLLI
                     ASPVLIIVALAIVATSGRPVFYRQTRVTSNGRPFTMLKFRTMPVDVEGQSGPVWSNPG
                     HPRSTRLGRLLRRYSLDELPQLINVLRGEMSLVGPRPERPEFIAQFRKDVPGYMQKHL
                     VKAGITGWAQVNDLRGSSDLPKRIQYDLYYIDNWSLWFDLRILGLTIMHIFRSRNAY"
BASE COUNT          351 a          588 c          622 g          305 t
ORIGIN      
        1 gcgaacagaa tctcggcgtc cagcgcctgg ccgccgtccc agcgcagctt cttttgcgcc
       61 ggatcgaact gcaccccttt gcgcctgaac accaccagat aatcgcctcc gcgcaacttc
      121 gcggcgtcgg gcatcgtgtt ccgatacgga tcgaagtaga cgttgtacgg atagaggtga
      181 taggcgccgc gatcccgaaa atacagcgag tccgcgccca tgaatacgcg caccggcggc
      241 ggcggaagct tcgcgcgcgc cttttcgatg aacgcgaaca atgcgccatc ctccgcggcg
      301 agatgacgct cgcgccatga cttgccgccg tattgctgga aagtcgcgcg cgcctcccgc
      361 accaggttcc attgccatgg gacatcgaga atgaaccacg cgcaaacgaa cagaccgccg
      421 gcgatcagcg gccggaacgg accgatcgtt tgcggccgcc accgcgccag gatgacataa
      481 atgatcaagg ccagccccac gatcgccgcc agggcaaagg gcaagggcag atcctgcgcg
      541 tccgccccgc cgctgcggct gttgatcgac ccgccgctcc atgactcgaa cgcgaaccat
      601 tcgcgaatgc ggtcgtgcag gacctgcacg gcagtcatcg gcttggcctc gacgccgcga
      661 atcatcatcg gctgagtcag atcgccgcgg atgatcacgg caaggccatt gatcttcccg
      721 atccaattgg gatcatcggc aagcgtgatc ggaagcaggc gtttcgcctc gacggtcagc
      781 ggtcgggtga aacggcgccc gggctcgtag tcgttgcgcc agatcagcgc cgcggcaacg
      841 tcgttgggga cgtcggccgt gtcccaggcg accgccgtgt atccggccga ccggaacgag
      901 gtctggatcg agacgatcgc gatgccttgc ttaccgggag cgctgacgcg aaggccatcg
      961 gcagtcggct ggccgctgcc ttgcgtcacg gcgagctcgg ccgcggtcca gcgcaaggta
     1021 ggcaagctgc cgaaccagct gccgggcacc gatagcgcca ggtagccgac gatgcacagc
     1081 aacgtgctgg ctgcgaatag cgaggctccg accagcatcg ctcgcggcgg cgcagcaggc
     1141 agcgctgtcg gcgcggcgat cgtttgcgga gccggctcgg cggcgggaga ggtcaagcgg
     1201 cgagaacggg ccctagtagg cgttgcggct gcgaaagatg tgcatgatgg tcaatccgag
     1261 aattcgcaga tcgaaccaca acgaccagtt gtcgatgtaa tagagatcgt attgtatccg
     1321 cttgggcagg tcgctgcttc cgcgcagatc gttgacctgc gcccatccgg tgatgccggc
     1381 cttgaccagg tgtttttgca tgtagccggg cacgtccttg cggaactggg cgatgaattc
     1441 cgggcgctcg gggcgcggac ccaccagcga catctctccg cgcagcacgt tgatcaactg
     1501 gggcagctcg tcgaggctgt accgccgaag caggcgaccg agtcgggtgc ttcgcgggtg
     1561 gccggggttc gaccagaccg gaccgctctg tccttcgaca tcgaccggca tcgtgcgaaa
     1621 tttgagcatg gtgaacgggc gcccgttcga cgtgacgcgc gtctgccgat aaaagaccgg
     1681 gcggccggat gtggcgacga tcgcgagggc gacaatgatc aatacagggg aggcgatcag
     1741 caggagcagc gcgccgagcg cgtagtcttc gccggccttg agcaggcggg ccgtgccctc
     1801 gatcggcgtg tcgctcaatc cgatgaccgg catgccggcg atttcggaaa agctgtgtcg
     1861 cagcag
//
LOCUS       DATANS010000180         2887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_262226, whole genome shotgun sequence.
ACCESSION   DATANS010000180 DATANS010000000
VERSION     DATANS010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262226"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="VHT22_02605"
     CDS             <1..1137
                     /locus_tag="VHT22_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03088 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HEX3136472.1"
                     /translation="GVVNLINRLPAQSWRHAVVSLTDFDPAFAARISSSTVRCIALHK
                     APGHAVRLYPRLVALLRELRPAIVHTRNLAALEATLPAWIAGVPARLHGEHGRDVGDL
                     DGSSLRYQRVRRLFRPFVTRYVALSPDLESYLRERIGVPATRIDQVYNGVDTARFFPS
                     PGGRAAIDACPFQGADYWLVGTVGRMEAVKDQTNLARAFVTAVAADPEARARLRLVLV
                     GDGKLKREIEAILESAGVRELAWFAGERTDVVPILQGLDCFVLPSLAEGVSNTILEAM
                     ACGLPVVATRVGANAELVEQNLTGRTVPAADSDALAREIGAYFATPGLARQHGHAARR
                     RVERRFSLERMVEDSDRVYAGLLRTRRPAHSGAAAAAPRPDVRS"
     gene            1157..>2887
                     /locus_tag="VHT22_02610"
     CDS             1157..>2887
                     /locus_tag="VHT22_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system amidotransferase"
                     /protein_id="HEX3136473.1"
                     /translation="MCGIVGMLDLNGRRDVSRELLSRINEAQHHRGPDECGLHVEPGV
                     GLGHRRLSIIDVSTGQQPLYNEDGSVVIVYNGEIYNYQELIPELTALGHRFRTKSDTE
                     VIVHAWEAWGERCVERFRGMFAFALWDRNRKTLFLARDRLGVKPLHYALLADGTFLFG
                     SEIKALLAHPAFVRDIDPHAVEEYFALGYVAEPRTIFKAAHKLPPAHTLCVRQGAPLP
                     APVQYWDPRFTLNRVITLPDAVAELTARLDESVRLRLISEVPLGAFLSGGVDSSAVVA
                     SMANASSDPVNTCSIAFADPAYDESRYAQQVADRYRTRHFVDRVESDDYDLIDTLARV
                     YDEPYADSSAIPTYRVCQLARKHVTVALSGDGGDENFGGYRRYRLHMMEERMRRLMPL
                     SLRRPVFGMLAGAYPKADWAPRMFRAKSTFEALGRDPVEAYCHGVSILRDGMRRRLFT
                     DGFRRELGGYSALEVFRRHAKSAQTEDPLALAQYLDLKTYLVGDINTKVDRASMAHSL
                     EVREPLMDHPLVEWLATLPSSFKVRGQEGKWLLKKALEPRLPAEILYRPKRGFAVPLA
                     RWFRGPLRTRV"
BASE COUNT          465 a          965 c          951 g          506 t
ORIGIN      
        1 ggcgtcgtca acctgatcaa ccgcctgccg gcgcaatcct ggcggcacgc agttgtgtcg
       61 cttaccgact tcgacccagc gttcgccgca cgcatctcca gcagcactgt ccgctgcatc
      121 gccttgcaca aggctccagg tcatgcggtc cgcctttatc cgaggctcgt cgcactgctt
      181 cgcgagctcc ggcccgcgat cgtgcacacc cggaacctgg ccgcgctcga ggccacgctc
      241 cccgcctgga ttgccggagt cccggctcgc ctgcacggcg agcatggtcg agacgtcggc
      301 gatctcgacg gctcgagcct gcgctaccag cgggtgcggc gcttgttccg tccattcgta
      361 acgcgctatg tcgcgctctc tcccgacctc gaatcctatt tgcgcgagcg catcggtgtg
      421 ccggcgacgc ggatcgatca ggtctacaac ggcgtcgaca ctgcgcgctt tttcccttcg
      481 cccggcgggc gcgccgcgat cgacgcctgt ccgttccagg gcgccgacta ctggctggta
      541 ggcaccgtcg gccggatgga agccgtcaag gaccagacca acctggcgag ggctttcgtc
      601 actgcggtcg ctgccgaccc cgaggcccgc gcccgcttgc ggctggtgct ggtcggcgac
      661 ggcaagttga aacgcgaaat cgaggcgata ctcgaatccg ccggcgtgcg cgagttggca
      721 tggtttgccg gcgaacgcac cgacgtggtc ccgatcctcc agggtctcga ttgcttcgtg
      781 ctgccgtccc tggcggaggg cgtttccaat acgatcctcg aggcgatggc ttgcggattg
      841 ccggtcgtcg cgacgcgggt gggtgccaac gcagaactgg tcgaacagaa cctgaccggg
      901 cgcacggtgc ccgcagctga cagcgacgcg ctggcgcgtg agatcggcgc gtatttcgcc
      961 acaccgggcc tcgcccggca gcatggccac gccgcacggc gccgagtcga acgccgattc
     1021 agcctcgagc gcatggtcga ggactccgac cgcgtctacg ccggcttgct tcgaacgcgt
     1081 cggcccgcgc attcgggcgc ggcggctgca gcgccgcgac cagacgttcg aagctagcgc
     1141 gaaggaaaag cgaatcatgt gcggcatcgt tggaatgctc gaccttaacg gcaggcgcga
     1201 cgtgagtcgc gaattgctgt cgcgcatcaa cgaggcccag catcaccgcg gccccgacga
     1261 gtgcgggctg cacgtcgaac caggcgtggg gctcggccat cgccgcctgt ccatcatcga
     1321 cgtgtccacc ggacagcagc cgctttacaa cgaggacggc agcgtggtga tcgtctacaa
     1381 cggcgagatc tacaattacc aggagctgat tccggagctg accgccctcg gtcatcgctt
     1441 ccgcaccaaa agcgacaccg aagtgatcgt ccatgcctgg gaggcgtggg gcgagcgatg
     1501 cgtcgagcgg tttcgcggga tgttcgcgtt cgcactgtgg gatcgcaacc gcaagacctt
     1561 gtttctggcc cgcgacagac tcggcgtcaa gccgctgcat tacgcgttgc tcgccgacgg
     1621 cacgttcctg ttcgggtccg aaatcaaggc gctgcttgcg catccggcct tcgtgcgcga
     1681 catcgacccg cacgcggtcg aggaatattt cgcgctcggt tatgtcgccg agccgcgcac
     1741 gatattcaag gcagcacaca aactgccgcc ggcgcacacg ctctgcgtgc gccagggtgc
     1801 gccgttgccg gcaccggtgc agtattggga cccgcgcttc acgctcaatc gtgtcatcac
     1861 gctgccggac gctgtggcgg agttgacggc ccggctcgac gaatcggtgc gtctgcgcct
     1921 gatttccgag gtcccgttgg gagcgttcct gtcgggcggc gtcgactcca gcgcggtggt
     1981 tgcctcgatg gcgaatgctt cgagcgatcc ggtcaacacc tgctcgatcg cgtttgccga
     2041 cccagcctac gacgaatccc gctatgcgca gcaggtcgcg gaccgttacc ggacgcggca
     2101 tttcgtcgat cgcgtcgaaa gcgacgacta cgacctgatc gacacgctgg cgcgcgtcta
     2161 cgacgagccg tacgcggaca gctcggcgat tcccacctac cgcgtctgcc agctggcgcg
     2221 caagcacgtc accgtcgcgc tgtcgggcga cggaggcgac gagaatttcg gcggctaccg
     2281 gcgctatcgc ctgcacatga tggaagagcg catgcggcgc ttgatgccgc tttcgctgcg
     2341 ccgaccggtg ttcggcatgc tcgctggcgc ttatccgaag gcggactggg cgccgcggat
     2401 gttccgcgcc aagtcgacct tcgaagcttt ggggcgcgat ccggtcgagg cgtactgtca
     2461 cggggtgtcg attctgcgcg acggcatgcg ccggcggctg ttcaccgacg gcttccgcag
     2521 ggagctcggc gggtacagcg cgctcgaggt attccggcga catgcaaaga gcgcgcaaac
     2581 cgaggacccg ttggcgctgg cccaatacct cgatctgaag acctatctgg tcggcgatat
     2641 caacaccaag gtcgaccgcg cgagcatggc ccactcgctc gaagtgcgag agcccctgat
     2701 ggaccatccg ctagtcgagt ggctggccac gctgccgtcg tcgttcaagg ttcggggcca
     2761 ggaagggaaa tggctgctga aaaaagcgct cgagccgcga ctgccggcgg agatcctgta
     2821 ccggccgaaa aggggctttg cggtcccgct ggcgcgctgg ttccgcggcc cgctgcgcac
     2881 acgggtg
//
LOCUS       DATANS010000181         2935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_94060, whole genome shotgun sequence.
ACCESSION   DATANS010000181 DATANS010000000
VERSION     DATANS010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94060"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /gene="murA"
                     /locus_tag="VHT22_02615"
     CDS             complement(<1..1073)
                     /gene="murA"
                     /locus_tag="VHT22_02615"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX3136474.1"
                     /translation="MDKLAITGGGPLQGEVRVSGAKNAALPILCAALLTSEPLVLTNV
                     PKLNDVRTMRSLLAQMGVHAEGETNALTLSAAEIDWPLAPYEMVKTMRASILALGPLL
                     ARCGEARVSLPGGCAIGLRPVDQHIKGLQAMGAEIDLDHGYIDAKAKRLSGATFVFDL
                     VTVTGTENLMMAATLADGTTVLDNAAREPEVVDLADCLNAMGARITGAGTDRIVIEGV
                     SRLSGATHAIMPDRIETGTFLVAAAATGGDVALTGARPDTLGAVLDKLAEAGVAPTID
                     GSTIRMKRNGRLKSVSMRTAPYPGFPTDMQAQLMALMTVADDAAMITETIFENRMMHV
                     QELKRLGAAIEVEGNTAIVRGAQ"
     gene            complement(1083..1331)
                     /locus_tag="VHT22_02620"
     CDS             complement(1083..1331)
                     /locus_tag="VHT22_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEX3136475.1"
                     /translation="MAAITPEEIKRTIESGLVCEHVAVDGDGRHFQALIVSGEFTGLS
                     KVRQHQLVYGVLGERMREEIHALSMQTLTPAQWRAPAG"
     gene            complement(1344..2156)
                     /locus_tag="VHT22_02625"
     CDS             complement(1344..2156)
                     /locus_tag="VHT22_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013204987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3136476.1"
                     /translation="MDQPTQGAARAVRLLPARGAGWLTLFYKELLRFWKVSFQTILAP
                     VLTALLYLLIFSHVLEAHVSVFGGRVSYTAFLIPGLVMMAVLQNAFANSSSSMIQSKI
                     TGNLIFVLLTPLSPLDMFAAYVLGAMVRGLVVGLGVFVVTLVFAPIAPAHPLWALAFA
                     LLGSAILGILGLIAGIWSDKFDQLAAFQNFVIVPLTFLSGVFYSIHSLPPFWQSLSHF
                     NPFFYMIDGFRYGFFGLSDFAPATSLAIVVFFCAALSAVTLVLLKRGYKLRH"
     gene            complement(2162..>2935)
                     /locus_tag="VHT22_02630"
     CDS             complement(2162..>2935)
                     /locus_tag="VHT22_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3136477.1"
                     /translation="KTTLISILAGLTRADRGDARVLGHDVVTDYARARRLLGVVPQEL
                     VFDPFFTVRETLRIQAGYFGIRDRDDWIDELLHHLGLASRANSNMRALSGGMKRRMLI
                     GQALVHKPPVIVLDEPTAGVDVELRQSLWSFIRKLNGDGHTIILTTHYLEEAESLCGR
                     IAMLKAGRIVALDTKQKLLSTFAGLTVRLTVAEQLPGAWPGRVLRRDGQTYLLRLADY
                     AELESMLAALREARITIIELSLQEADLEQVFLRIMARQE"
BASE COUNT          539 a          986 c          934 g          476 t
ORIGIN      
        1 ttttgcgccc cgcggacaat cgcggtgttg ccttcaacct cgatcgccgc acccaggcgc
       61 ttcaactcct gcacgtgcat catgcggttc tcgaatatcg tttccgtgat catcgccgcg
      121 tcgtcggcca cggtcatcag cgccatcagc tgggcctgca tgtcggtcgg aaaccccgga
      181 tagggcgcgg tgcgcatcga taccgacttg agccttccgt tgcgcttcat ccggatcgtg
      241 ctcccgtcta tcgtcggcgc tacgcccgct tcggcgagct tgtccaggac ggcacccagc
      301 gtatcgggac gcgctccggt aagcgcgacg tcgccgccgg tcgcggcggc ggcgaccagg
      361 aaggttccgg tctcgatacg atcgggcatg atcgcgtgcg tggcgccgga gaggcgcgac
      421 accccttcga tcacgatgcg gtcggtgccg gcgccggtga tcctggcccc catcgcgttc
      481 aggcagtcgg cgagatcgac gacctccggt tcgcgcgccg cattgtccag caccgtcgta
      541 ccgtccgcga gcgtcgcggc catcatcagg ttttcggtgc cggtcaccgt caccaggtcg
      601 aagacgaaag tggcgccgga cagacgcttc gccttggcat cgatgtagcc gtgatcgaga
      661 tcgatttccg cgcccatcgc ctgcaggcct ttgatgtgct ggtcgaccgg ccgcagcccg
      721 atcgcgcagc cgcccggcag cgacacgcgc gcttcgccgc agcgcgccag cagcggtccg
      781 agcgccagga tcgacgcgcg catcgtcttc accatctcgt aaggcgcgag cggccagtcg
      841 atttcggccg ccgagagcgt cagcgcgttg gtctcccctt ccgcgtgcac acccatctgc
      901 gcgagcagcg agcgcatggt gcgcacgtcg ttgagcttcg gcacgttcgt gagcaccagc
      961 ggctcggagg tcagcagcgc ggcgcacagg ataggcagcg ccgcattctt ggcgccggaa
     1021 acccgcactt cgccttgcag cggaccaccg ccggtaatcg ccagcttgtc catcggcgcc
     1081 agctaccccg ccggagcgcg ccactgggcc ggcgtcagcg tctgcatcga cagcgcatga
     1141 atttcctcgc gcatgcgctc gcccaggacg ccgtagacga gctggtgctg ccgcaccttg
     1201 ctcaagccgg tgaattcccc gctgacgatc agcgcctgga aatgccgccc gtcgccgtcg
     1261 accgcgacgt gctcgcagac gagccccgac tcgatcgtgc gcttgatttc ttccggagtg
     1321 atcgccgcca tgttgggacc tggtcaatgc cgaagtttgt agccgcgctt gagcaggacc
     1381 agcgttaccg cgctcaatgc tgcgcagaag aagacgacaa tcgcgaggct cgtcgccggc
     1441 gcgaaatccg acaggccgaa aaagccgtag cggaagccgt cgatcatata gaagaacgga
     1501 ttgaaatgcg acagcgactg ccagaacggc ggcagcgaat ggatcgagta gaagacgccg
     1561 gacaggaacg tcagcggcac gatgacgaag ttctggaaag ccgccagctg atcgaacttg
     1621 tcggaccaga ttcccgcgat caatcccaga atgcccagga tggcgctgcc gagcagcgcg
     1681 aaggcgagcg cccatagcgg atgcgcgggc gcgatcggtg caaacaccag cgtgacgacg
     1741 aacaccccca ggccgacgac caggccgcgc accatcgcgc ccagcacgta ggcggcgaac
     1801 atgtcgagcg gcgacagtgg agtcagcagg acgaagatca ggttgccggt gatcttcgac
     1861 tggatcatcg acgacgagct gttggcgaac gcattctgca ggactgccat catcaccagg
     1921 ccgggaatca ggaacgccgt ataggaaacg cgtccgccga aaaccgaaac atgcgcctcg
     1981 aggacatgcg aaaaaatcag caggtagagg agcgccgtca gcaccggcgc gagtatcgtc
     2041 tggaagctga ccttccagaa acgcaacagc tccttgtaga acagcgtcag ccagcccgcg
     2101 ccgcgcgccg gcagaagtcg caccgcgcgg gccgcacctt gcgtcggctg atcgatcgcc
     2161 ttcactcctg gcgcgccatg atgcgcagga acacctgctc gaggtcggct tcctgcagcg
     2221 acagctcgat gatcgtgatt cgcgcttcgc gcagcgccgc cagcatgctc tccagctcgg
     2281 cgtaatcggc cagcctcaac agatacgtct gcccgtcgcg gcgcagaacc cggccgggcc
     2341 acgcgcccgg cagttgctcg gcgacggtca gccggacggt gagtccggcg aaggtcgaca
     2401 gcagcttctg cttcgtgtcg agcgcaacga tcctgccggc cttgagcatc gcaatgcgtc
     2461 cgcaaaggga ctccgcttcc tccagatagt gcgtcgtgag gatgatcgtg tggccgtcgc
     2521 cgttgagctt gcggatgaac gaccacaggc tctgccgcaa ttcgacgtcg acaccggcgg
     2581 taggctcgtc gaggacgatt accggcggct tgtgcaccag cgcctgtccg atcagcatgc
     2641 ggcgcttcat cccgccggac agcgcccgca tgttgctgtt ggcgcgcgaa gcgaggccca
     2701 ggtggtgcag cagctcgtcg atccagtcat cccgatcgcg aatgccgaaa tagccggcct
     2761 ggatgcgcag cgtctctcgc acggtgaaaa acggatcgaa caccagttcc tgcggtacga
     2821 cgcccagcag gcggcgcgca cgcgcgtagt cggtgaccac gtcgtgaccc agcacgcgcg
     2881 cgtcgccccg gtcggcacgc gtgagccccg cgagaatcga gatcagcgtg gtttt
//
LOCUS       DATANS010000182         1686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_345337, whole genome shotgun sequence.
ACCESSION   DATANS010000182 DATANS010000000
VERSION     DATANS010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345337"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VHT22_02635"
     CDS             <1..288
                     /locus_tag="VHT22_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX3136478.1"
                     /translation="GLEIHGILRTMFDPRNNLANEVSAQLVEHFREKVFRTIIPRNVR
                     LAEAPSYGKPALHYDRESRGALAYLALAGEMIRRDDGDYAAAAAGSDVVAQ"
     gene            317..1231
                     /locus_tag="VHT22_02640"
     CDS             317..1231
                     /locus_tag="VHT22_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002814291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX3136479.1"
                     /translation="MAIMGTTKRRSLGRGLGALLGQPEPDDESPVVTKLHSDEELLKV
                     PVDLLQRGKYQPRIDMRPETLQELADSIKAQGVVQPIIVRPIDTPEGAGHARYEIIAG
                     ERRWRAAQLAGLHEIPAVVRRVADDAAIAMALIENIQRENLNPLEEARALDRLITEFD
                     MTHATAAEAVGRSRAAVSNLLRLLELTDEVKALVEQRELEMGHARALLGLETRRQQAE
                     VGALVAKKKLSVRETEALVKRLTTQPQAAAEESASRVDPNIRKLESQLSEKLGAKVAV
                     QHTASGKGKLVISYHSLDELDGIIDHIR"
     gene            1421..>1686
                     /locus_tag="VHT22_02645"
     CDS             1421..>1686
                     /locus_tag="VHT22_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136480.1"
                     /translation="MSTNVLAAGARQSVRILLWQALWSGALATIFAVTLGTKAAVSAL
                     VGGGIGLVWTVYMALVLFKHSLTHGVRLSAISFFAGWLFKVVVTV"
BASE COUNT          318 a          519 c          562 g          287 t
ORIGIN      
        1 ggcctggaga tccacggcat cctgcgcacc atgttcgatc cgcgtaacaa cctcgccaac
       61 gaagtatcgg cgcagctcgt cgagcacttc cgcgagaagg tgtttcgcac gatcattcct
      121 cgcaacgtgc ggctggcgga agccccgagt tacggcaagc cggcactgca ttacgatcgc
      181 gagtcgcggg gggcgctcgc ctacctggcg ctcgcggggg aaatgatccg gcgtgatgac
      241 ggcgactacg cggctgcggc tgcgggcagc gacgtcgtcg cgcagtaatt cgcactgaaa
      301 ttccggacaa acaagaatgg caatcatggg gacgaccaaa cgacgaagcc tcggccgtgg
      361 actgggcgca ctgctcggtc agcccgagcc ggacgatgag tccccggtcg tcaccaagct
      421 gcactccgac gaggagctgc tgaaggtgcc ggtcgacctg ctccagcgcg gcaaatatca
      481 gccgcgcatc gacatgcggc ccgaaacgct gcaggagctg gccgactcga tcaaagcgca
      541 aggtgtcgta cagcctatca tcgtgcgacc gatcgatacg ccggaaggtg ccgggcacgc
      601 acgctacgaa atcatcgctg gcgagcgccg ctggcgcgcc gcgcagctgg ccggcctgca
      661 cgagattccg gccgtcgtcc gccgcgtcgc cgatgacgcc gcgatcgcga tggcgctgat
      721 cgagaacatc cagcgcgaga acctgaatcc gctggaagaa gcacgggcgc tggatcgcct
      781 gatcaccgaa ttcgacatga cgcatgcgac ggctgccgag gccgtcggcc gttcgcgcgc
      841 ggccgtctcc aatctgctgc gcctgctgga gcttaccgac gaggtaaagg ccctggtcga
      901 gcagcgcgag ctggaaatgg gtcatgcgcg tgccctgctc gggctcgaaa cgcggcggca
      961 gcaggcggaa gtcggcgcgc tggtcgccaa gaagaagctg tcggtgcggg aaaccgaggc
     1021 gctggtcaaa cggctcacca cgcagccgca agctgcggcc gaggagagcg cgtcgcgcgt
     1081 cgatccgaac atccgcaagc tggaaagcca gttgagcgag aagctgggcg ccaaagtcgc
     1141 cgtgcaacac acggcttcgg gcaagggcaa gctggtgatc agctatcact cgctcgacga
     1201 gctcgacggg atcatcgacc acatccgctg agcggtttct gtaaagcgcc cggcccgccc
     1261 gaagtttctc tatctctttg ttgaagcagc gagaatcgct gcgtataatg cgccgccttt
     1321 tccggccggg cacacatctc ggctggcatt ttcttcgggt cgaaacaatg ccacacgtgc
     1381 cgatcgctcg gctgcaagca cggtggcatt tttgttgtcc gtgagtacca acgttcttgc
     1441 agccggcgcg cgccagtcag tacgaattct gctgtggcag gcgttgtgga gcggagcgct
     1501 cgccacaata tttgcggtga cgctcggcac gaaggccgcg gtgtcggcgc tggtgggcgg
     1561 cggcatcggc ctggtctgga cggtgtacat ggcgttggtg ctgttcaagc acagcctgac
     1621 gcacggcgtg cgcctgagtg cgatcagttt tttcgcagga tggctgttca aggtcgtggt
     1681 gacggt
//
LOCUS       DATANS010000183         1965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_262358, whole genome shotgun sequence.
ACCESSION   DATANS010000183 DATANS010000000
VERSION     DATANS010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262358"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VHT22_02650"
     CDS             <1..270
                     /locus_tag="VHT22_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136481.1"
                     /translation="TSAAGAFDAVDGHPLTVQQTFDRLGAVCDVPSSMQPETVVKDVL
                     DEHTVAVMHRDIALRSTRAQEIGWSPRYRTFNEGAAPAYAQWCAQ"
     gene            267..1424
                     /locus_tag="VHT22_02655"
     CDS             267..1424
                     /locus_tag="VHT22_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136482.1"
                     /translation="MILQALVLCAAAAVADPIAIYDEAVAARKDRDATRLLQLTGQLI
                     EYAPASPPLRFLHAEALALSGQTQPALAELRWLADHGYDYAFWERDSFASLRVSETAS
                     LRAAMTKNGAPAGRIARVVNLDRTDLDPEGIAALGQDWIVGSMANGSLYRVNRAGATT
                     VLWHETEAGRRVFGVRHDPQRNLLWACSNGPDDRRARPQLLRVALEPLAVTRLPVPDA
                     RALCNDIALLPEGAVAISDSQRGAVWQLANDGSWRALAKPGALGYPNGLAYLPETRQL
                     VVADLRGLWTMDLAASRLTPVTEQGTFIGGIDGLYAVDGELVTIQNGLRPHRVLRIVL
                     SPNAGRVLSVAAIASGVPELAAMTTAAVARREIVVLAGSQLVVVSSGSAGD"
     gene            complement(1399..>1965)
                     /locus_tag="VHT22_02660"
     CDS             complement(1399..>1965)
                     /locus_tag="VHT22_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX3136483.1"
                     /translation="PIHTQRKDIPEELEEIVATAMLKTPDKRQKSGADFAAELTRVHQ
                     RLREQNARVDQQAQFDVLRRLRFFHEFSQTEIWEVLRASTMSEYPAGEEIVKEGEMDD
                     RFYVIVSGSVAVMRNSRAVGTLDAGDCFGETSYVRGAKRLATIKALSPVTLMKVSSTL
                     LEQSSAACQLRFNKVFLRSLISRLQSPS"
BASE COUNT          317 a          640 c          656 g          352 t
ORIGIN      
        1 acttcagcag cgggcgcctt cgatgcagtc gacgggcatc cgctgactgt gcaacaaacg
       61 ttcgaccgcc tgggtgcggt gtgtgacgtg ccgtcgtcga tgcaacccga aactgtcgtg
      121 aaagatgttc tggatgagca caccgtcgcc gtcatgcatc gcgacatcgc tctgcgctcc
      181 acgcgcgcgc aggagatcgg ctggtcgccg cgctatcgga ccttcaatga aggcgcggcg
      241 cctgcctacg cccagtggtg tgcgcaatga tcctgcaagc gctcgtactg tgcgcggccg
      301 cggcagtcgc cgatccgatc gcgatctacg acgaagcggt agccgcgcgc aaggatcgcg
      361 atgctacgcg cttgctgcag ctgactgggc agctgatcga gtacgcgccg gcgagtccgc
      421 cgctgcgctt cttgcacgcc gaagcactcg ccctctcggg acaaacccag ccggcactcg
      481 ccgagctgcg ttggcttgcg gaccatggtt atgactacgc gttctgggaa cgcgactcct
      541 ttgccagttt gcgagtcagc gaaacagctt cgttgcgcgc agcgatgacg aagaacggcg
      601 cgcccgcggg acgaatcgca cgcgtcgtga acctcgaccg caccgatctc gatccggaag
      661 gcatcgctgc gctcgggcaa gattggatcg tcggctcgat ggccaacggc agcctgtatc
      721 gcgtgaatcg cgcgggagcg accactgtgt tgtggcacga gaccgaggcg ggccgccgcg
      781 tgttcggcgt gcgtcacgac ccgcagcgca atctcctctg ggcgtgctcc aacggtcccg
      841 atgaccggcg cgcgcggccg cagttgctgc gtgtcgcgct ggagcctctg gcagttactc
      901 gcctgccggt accggatgct cgcgccttgt gcaacgacat cgcgctgctc ccggagggcg
      961 ccgtcgcgat ctcggactcg caacgcgggg cagtctggca gctcgccaac gacggcagct
     1021 ggcgagcgct ggcaaagccc ggcgcgctcg gctatccgaa cggcctcgcc tacttgcctg
     1081 aaacgcggca gctcgtggtc gcggacctgc gagggctgtg gacgatggac ctggccgcgt
     1141 cgcggctgac gcccgtaacg gagcaaggaa ccttcatcgg cgggatcgac gggctttatg
     1201 ccgtcgacgg cgaactggta acgatccaga acggcctgcg accgcatcgt gtgctgcgca
     1261 tcgtcctgag ccccaacgca ggacgggtgt tgagcgttgc tgcaatcgca tcaggtgtgc
     1321 cggaattggc cgcgatgacg accgcagcag tggcacgacg ggagatcgta gtgctggccg
     1381 gcagccagct ggtggtcgtc agctcgggct ctgcaggcga ctgatcaggc tgcgcaggaa
     1441 gactttgtta aatcgcagct gacacgcggc tgacgactgc tcgagcagcg tcgagctcac
     1501 cttcatcagc gtgaccggcg acagtgcctt gatcgtcgcc agtcgcttcg ctccgcgcac
     1561 gtagctggtc tcgccgaagc agtcgcctgc gtccagcgtg ccgacggcgc gactgttgcg
     1621 catcactgcg acgctgccgg acacgatcac gtagaagcga tcgtccatct cgccttcctt
     1681 gacgatctcc tcgcccgccg ggtactcgct catggtgctg gcgcgcagca cttcccagat
     1741 ttcggtctgc gagaactcgt ggaagaagcg caggcggcgc aggacgtcga attgcgcctg
     1801 ctggtcgacg cgggcgttct gctcgcgcag gcgctggtgc acacgcgtga gctcggccgc
     1861 gaaatcggcg cccgacttct ggcgcttgtc cggagtcttg agcatcgcgg tggcgacgat
     1921 ctcctcgagc tcctccggaa tgtccttgcg ctgcgtgtgg atcgg
//
LOCUS       DATANS010000184         1740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_304287, whole genome shotgun sequence.
ACCESSION   DATANS010000184 DATANS010000000
VERSION     DATANS010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304287"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="VHT22_02665"
     CDS             <1..1123
                     /locus_tag="VHT22_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEX3136484.1"
                     /translation="GLSASLEQVKKQAGSTNTQDVQLRALEREAKAQRDLLESYLAKY
                     REANTRENIEAPADGRIISRASVSNTPAYPKKLPIVLIATLATLLLTSGAIATGELLR
                     MTAPAARNADAPRVAFEAEPTFVASHPPEPVRAPAITPELPAAPAVEPDDFAEPELIE
                     PVMDAPRMDAAVTASEIEEMADMLACAPAAARKVTVLGTASSDSITLTALTLARLMAR
                     EARVVVVDLVASSPTLAAVSSDPMAPGLAELMEGEASFAQIITRDRQSRVHLVSAGRP
                     GFDRAHLQSPRLTLAIDALLRVYDHVLLDAGAASDLSAELLTWQARAVVVPDESMTQD
                     ARDQMCDQLRAVGFSEVTMLSKSFRVSDAIEQDPHVVAA"
     gene            complement(1237..>1740)
                     /locus_tag="VHT22_02670"
     CDS             complement(1237..>1740)
                     /locus_tag="VHT22_02670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3136485.1"
                     /translation="TIKPQRMAAQQLPDVFADPGIEDFIRDACTTELADGGHAIDIHA
                     LECDDEMIAIYAGVADDHRFSMMFNTYTMSGNARNSPGLILMRDIIDHYAGQGYRALD
                     LGIGSDDYKRLFCKDDEPIFDSFIPLSQRGKLAASVMSGLNRTKRLVKHNPALLEMAQ
                     KLRNAFS"
BASE COUNT          295 a          553 c          588 g          304 t
ORIGIN      
        1 aggcctcagc gccagcctgg aacaggtgaa aaaacaggcg ggctcgacca atacccagga
       61 cgtgcagctg cgcgcgctcg agcgcgaggc caaggcgcag cgcgacctgc tggaatccta
      121 tctggcgaaa taccgcgagg cgaacacccg cgaaaacatc gaggcgccgg ccgacggccg
      181 catcatctcg cgcgccagcg tttccaacac cccggcctat ccgaagaagc tgccgatcgt
      241 gctgatcgcg acgctggcga ccttgctgct cacctcgggc gcgattgcga ccggcgagct
      301 gttgcgcatg accgcgccgg cggcccgcaa tgcggatgcg ccgcgagttg cgttcgaggc
      361 cgagcccacc tttgtagcgt ctcatccgcc ggagccggtg cgcgcacccg cgatcacgcc
      421 cgagcttccc gccgcgccgg ccgtcgaacc cgacgacttt gcggaaccgg agctgatcga
      481 gcccgtgatg gatgcgccgc ggatggatgc cgcggtgaca gccagcgaaa tcgaggagat
      541 ggcggatatg ctggcttgcg cccccgccgc ggcgcgcaag gtgaccgtgc tgggcacggc
      601 atcgagcgac agcatcacgc tgaccgcgct gacgctggcg cggctgatgg cgcgggaggc
      661 gagagtcgta gtcgtcgacc tcgtggcatc ctcgccgacc cttgcggcgg tgtcgtcaga
      721 tccgatggcg cccggccttg ccgaattgat ggaaggcgag gcgtcgtttg cgcaaatcat
      781 cactcgggac cggcagtcac gcgtccatct cgtcagcgcc ggtcgtccgg gcttcgaccg
      841 cgcgcatctc cagtcgccgc gactgacgct cgcgatcgac gcgctgctgc gagtctacga
      901 ccatgtgctg ctggatgccg gtgcggcatc cgatttgtcg gccgagctgc tgacctggca
      961 ggcgcgggcc gtcgtggtgc ccgacgaatc gatgacgcag gacgcccgcg atcagatgtg
     1021 cgaccagctc agggcggtcg gcttttccga agtgacgatg ctgagcaagt cgttccgggt
     1081 gtcggatgcg atcgagcaag acccgcacgt ggtggcggcc tgacaaaatt tgtagggtgg
     1141 gcaaagcgaa gcgtgcccac cgtccaagct tgtgacatcg gatagatggt gggcacggcg
     1201 ctgcgcgcct ttgcccaccc tacaagtgca acttgcctag ctgaacgcgt tgcgcagctt
     1261 ttgcgccatc tccagcagtg ccggattgtg cttcaccagc cgcttggtgc ggttgaggcc
     1321 ggacatgacg ctggcggcaa gcttgccgcg ctggctgagc gggatgaagc tgtcgaagat
     1381 cggctcgtcg tccttgcaga acagccgctt gtaatcgtcc gatccgatcc ccaggtcgag
     1441 ggcgcgataa ccctgtccgg catagtgatc gatgatgtcg cgcatcagga tcaggccggg
     1501 actgttccgc gcattgccgg acatggtgta ggtgttgaac atcatcgaga accggtgatc
     1561 gtcggcgacg ccggcgtaga tcgcgatcat ttcgtcgtcg cattccagcg cgtggatgtc
     1621 gatggcgtgg ccgccgtccg caagctcggt cgtgcaggcg tcgcggatga aatcctcgat
     1681 accgggatcg gcgaacacgt cgggcagttg ctgcgccgcc atccgctgcg gttttatcgt
//
LOCUS       DATANS010000185         2007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_262483, whole genome shotgun sequence.
ACCESSION   DATANS010000185 DATANS010000000
VERSION     DATANS010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262483"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VHT22_02675"
     CDS             complement(<1..212)
                     /locus_tag="VHT22_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011589090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136486.1"
                     /translation="MMQQYLRIKAEHADKLVFYRMGDFYELFYGDAQRAAPLLDITLT
                     ARGQSAGAPIPMAGVPYHAVEQYLAKL"
     gene            complement(299..1612)
                     /locus_tag="VHT22_02680"
     CDS             complement(299..1612)
                     /locus_tag="VHT22_02680"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEX3136487.1"
                     /translation="MRAHPFLVVLTCVLALALGSCNTGKGVSNGSTHGTLRVLNVIPN
                     AGGPLNVTFDLKPFVTDLPFEGMTQYQTIDAGSREVQVSVAGSSTNLIDVTPIFLPDV
                     AYTFVAFGALANATGIILNDTLIVDPGAGMFALRVVNGASNNGGIDVYVTAPGEDLNT
                     VSPSIAGIGYGTTSLFVNLPVGHREIRITPANSKQVIYDGPAQDFAERAHVQAVVYSR
                     GSSTLVNVALLNIDDTGTGAIANSSLARFRVINGSSVGSPLNVFVDHDLALSNIPYAA
                     ASSYQTINAGQRLLTVESTATPGAVLLTLTPTLLPASDTAIAITGAAGALSATLYSEP
                     SFPPSLVRARVRFVNSTMDVAALDVFVNFSLQVSGLPMNAISAGIELDADAIAGTAYE
                     FDFKASGSSQVGLQLPGVVLFGGQLYTIYVVGPGSALAGVVVQDN"
     gene            complement(1753..>2007)
                     /locus_tag="VHT22_02685"
     CDS             complement(1753..>2007)
                     /locus_tag="VHT22_02685"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEX3136488.1"
                     /translation="VAAFDVFVNFSKQVSGLSTNSASGYINLSAAASTGTSYEFDFNL
                     AGTTTSLLKLTSVVLTAGHTYTVYLAGPSSSLRGIVSQDN"
BASE COUNT          375 a          621 c          655 g          356 t
ORIGIN      
        1 agcttggcca gatactgctc gaccgcgtga tagggtacgc cggccattgg aatcggcgcg
       61 cccgccgatt gtccgcgcgc ggtcagggtg atatcgagca gcggcgccgc gcgctgcgca
      121 tcgccgtaga aaagctcgta gaagtcgccc atgcggtaaa acacgagctt gtcggcatgc
      181 tccgccttga tgcgcaaata ttgctgcatc atcggcgtat gcgccgcata ggcgccggcg
      241 gcttcggctg gcgtcagttc gttgtgcaag agcgagctgc caatcgcccg aaccggcctt
      301 agttatcctg gacgacgacg ccggcgagcg cggagcccgg tccgaccaca taaatcgtgt
      361 acagctgtcc gccgaacagc accaccccgg gaagctgcag gccgacctgg ctcgatccgc
      421 tggccttgaa gtcgaactcg tacgcagttc ccgctatcgc gtcggcatcg agctcgatac
      481 cagccgagat ggcgttcatc ggcaggccgg agacctgcag gctgaagttg acgaacacat
      541 cgagcgccgc aacgtccatc gtactgttga cgaagcggac gcgggcacga accagcgacg
      601 gcgggaagct gggttcgctg tacagcgtcg cgctcaacgc cccggccgcg ccggtgatcg
      661 cgatcgccgt atcgctcgcc gggagaagcg tcggcgtgag cgtcagcagc accgcgcccg
      721 gcgtcgccgt cgattccacg gtcagcagcc tctgtccggc attgatcgtc tggtagctcg
      781 atgcggccgc gtaagggatg ttcgacagcg caagatcatg atcgacgaaa acattcaacg
      841 gcgaacccac cgatgagccg ttgatcaccc tgaaacgggc gagcgagcta ttggcgatcg
      901 ctccagtgcc ggtgtcgtct atgttgagaa gggcgacatt gaccagcgta ctgctgccgc
      961 gcgaatagac gaccgcctgg acgtgagcgc gctcggcgaa gtcctgcgcc ggcccatcgt
     1021 agatgacctg cttggagttg gccggggtaa tccgaatttc gcgatgaccc accggcagat
     1081 tgacgaacag gctggtggtg ccgtatccga tgccggcgat gctcggcgag acggtgttga
     1141 ggtcttcgcc gggcgccgtg acgtagacat cgattccgcc gttgttgctc gcgccgttga
     1201 ccacccgcag cgcgaacatg ccggcacccg gatcgacgat caacgtgtcg ttgaggatga
     1261 taccggtggc gttggcgagt gcgccgaacg ccacaaacgt atatgcgacg tcgggcagaa
     1321 aaatcggtgt cacgtcgatc agattggtcg agcttccggc gacgctcacc tgcacttcgc
     1381 gcgatccggc gtcgatcgtc tggtactggg tcattccctc gaacggcagg tccgtcacga
     1441 acggcttcaa gtcgaatgtg acgttcaacg ggccgccggc gttcgggatg acgttgagca
     1501 ctcgcagcgt gccatgcgtg ctgccgttgc tgacaccctt gcccgtgttg cagctgccca
     1561 gcgcgagcgc caggacgcaa gtcaaaacaa cgagaaatgg gtgcgcacgc atcgtttgct
     1621 ccggatcagt gaacctgctc aagccgcgcc gcttgtcacg gccgccgcca tccggcgtat
     1681 cgctcccgcc gggcgcgcgc gtgggttgtc cgcgcggcgc gaattcgacg gtggccgcgg
     1741 ccaggcgatc gctcagttgt cctgcgagac gatgccgcgc aggctgctgg acggaccggc
     1801 gagatagacc gtataggtat ggcccgcggt gagcaccacg gacgtcagct tcagcaacga
     1861 ggtcgtcgtc ccggccagat tgaagtcgaa ttcatagctg gttccggtcg aggcggccgc
     1921 gctgagattg atgtatcccg aagcgctatt ggtggaaagg ccggaaacct gcttgctgaa
     1981 attgacgaac acgtcgaacg ccgcgac
//
LOCUS       DATANS010000186         2599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_596783, whole genome shotgun sequence.
ACCESSION   DATANS010000186 DATANS010000000
VERSION     DATANS010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596783"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VHT22_02690"
     CDS             <1..435
                     /locus_tag="VHT22_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEX3136489.1"
                     /translation="VDGAIHRAAGPELLAECRTLGGCPTGQAKITRGYRLPARYVIHT
                     VGPVWNGGRRGEPALLASCYRASLKLAREHAVRSLAFPAISCGVYRYPPAAAVKIAVS
                     ECAAAVDSGALPAEIIFACFDQAMLNLYNAELANAGAAKGAE"
     gene            466..1125
                     /locus_tag="VHT22_02695"
     CDS             466..1125
                     /locus_tag="VHT22_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3136490.1"
                     /translation="MIAAFLKTCFRREPAAFVPLDGVRRLNVGCGTDIRDGWINLDSS
                     RLPGVDVVANLDDCGRTPLPLPADSIDEFYCSHVLEHLRDALGFMQELHRIAKPGAIA
                     TIRVPYGSSDDADEDPTHVRRLYLYSFSYFSQRGYWYADYGYRGDWEVDNIVLTVDAA
                     RHAGKTFDQIFEELRSQRNVVLEMIATLTAVKPARPRGLRDKSLNMRIEFKHIATARP
                     F"
     gene            1136..>2599
                     /locus_tag="VHT22_02700"
     CDS             1136..>2599
                     /locus_tag="VHT22_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX3136491.1"
                     /translation="MAQFRSRLKPGTAEFTAAAAQMRGHADELKRKLAAIGRGGDDAS
                     RERHIARGKLLVRDRIKALLDPESPWLELSPLAAYEMYENAVPAAGIVTGIGRVMCRE
                     CVVIANDATVKGGTYFPLTVKKHLRAQEIAQVNRLPCIYLVDSGGAHLPSQDEVFPDR
                     DHFGRIFFNQANMSALGIAQVAVVMGSCTAGGAYVPAMSDETIIVKNQGTIFLAGPPL
                     VKAATGEIVTAEELGGGDVHTKISGVADHLADDDAHALQIARRAIGHLNIVKPAALDM
                     REPRAPALAPAELYGLIPVNPRAQFDVREIIARIVDGSEFDEFKARYGATLVTAFAHI
                     EGHPVGIVANNGILFSESALKGTHFIELCSQRGIPLVFLQNINGFMVGKKYEAGGIAK
                     DGAKMVTAVACSRVPKLTVIVGGSFGAGNYGMCGRAFDPRFVWMWPNARISVMGGEQA
                     ANVLAQVKRDALEKAGQTWSAGDENDFKAPILEQYAQQ"
BASE COUNT          490 a          840 c          817 g          452 t
ORIGIN      
        1 gtcgacggcg caatccatcg cgcggcgggc cccgaactgc ttgcggaatg ccgcacgctc
       61 ggcggctgtc cgaccggcca ggcgaaaatc acgcgcggat accggctgcc cgcgcgctac
      121 gtgattcata ccgtggggcc ggtgtggaac ggcggccggc gcggcgaacc cgcgctgctc
      181 gcgtcgtgct atcgcgcaag cctcaagctt gcgcgcgagc acgccgtgcg ctcgctcgca
      241 tttcccgcca tcagttgcgg cgtctaccgc tatccgcccg cagccgcggt caaaatcgcc
      301 gtcagcgaat gcgcggccgc cgttgacagc ggcgcgcttc cggccgagat tatctttgcg
      361 tgcttcgatc aagcgatgtt gaacctatac aatgccgagc ttgcgaatgc gggagccgcg
      421 aagggcgcgg agtaaatcga tgcgacagcc acgaccggcg gccgggtgat tgccgctttc
      481 ctcaagacct gcttccggcg tgagccggca gcctttgtgc ctcttgatgg cgtccgacgc
      541 ctgaacgtag gctgcgggac cgacatccgc gacggctgga tcaatctcga ttcgagccgg
      601 ctgcccggcg tcgacgtcgt cgccaatctc gacgattgcg gccgcacgcc gctgccgttg
      661 cccgcggatt cgatcgacga gttctactgc agccacgtgc tcgagcacct gcgcgacgcg
      721 ctcggattca tgcaggaact gcatcggatc gcgaaacccg gcgccattgc gaccatccgc
      781 gtgccgtacg gctcgagcga tgacgccgac gaagacccga cccacgtcag gcggctgtat
      841 ctttattcgt tctcctattt ttcccagcgc ggctactggt acgcggacta tggttaccgc
      901 ggcgactggg aagtcgacaa catcgtattg accgtcgatg ccgcgcgcca cgcgggaaaa
      961 acgttcgatc agatcttcga agagctgcgt tcgcaacgca atgtcgtgct ggaaatgatc
     1021 gcgacgctca cagcggtcaa gccggcgcgc ccgcgcggat tgcgcgacaa atcgttgaac
     1081 atgcggatcg aattcaagca catcgcgacc gcgcggccgt tctgagttaa ccgtcatggc
     1141 tcaattccgc tcgcgcttaa aaccaggtac cgcagaattc accgcggccg cggcacagat
     1201 gcgcggccac gccgatgaac tgaagcgcaa gctcgcagcg atcggccggg gcggcgacga
     1261 cgcatcgcgc gagcgccata tcgcgcgcgg taaattgctc gtgcgcgacc gcatcaaggc
     1321 attgctcgat ccggagtcgc catggctgga gctgtcgccg ctcgccgcct atgaaatgta
     1381 cgaaaatgcg gtacctgccg ccggcatcgt gaccggcatc ggccgcgtaa tgtgccgcga
     1441 atgcgtggtc attgcgaacg atgcgacggt caaaggcggc acgtactttc cgctgacggt
     1501 caaaaagcat ttgcgcgccc aggaaatcgc gcaggtgaac cgcctgccgt gcatctatct
     1561 cgtagactcg ggcggcgcgc acttgccgag ccaggacgaa gtctttcccg atcgtgatca
     1621 cttcgggcgc atattcttca accaggcaaa catgtcggcg ctcggcatcg cgcaggttgc
     1681 cgtcgtaatg ggctcgtgca ccgcgggcgg cgcttacgtg ccggcgatgt cggacgagac
     1741 gatcatcgtc aaaaatcagg gcaccatatt tctggccgga ccgccactcg tcaaagcagc
     1801 gaccggcgaa atcgtcaccg cggaagagct gggcggcggg gatgtgcata ccaaaatctc
     1861 gggcgtcgcc gatcatctcg ccgacgacga tgcgcatgcc ttgcaaattg cgcgccgcgc
     1921 aatcggccac ctgaacatcg tcaagcccgc cgcgctcgat atgcgcgagc cgcgcgcgcc
     1981 ggcgctcgct cccgcggaac tgtacggtct gattccagtc aacccgcgcg cgcaattcga
     2041 cgtgcgcgaa atcatcgcgc gcattgtcga cggttccgag ttcgacgaat tcaaggcgcg
     2101 ctacggcgcc acgctggtca ccgcgttcgc ccacatagag ggccacccgg tcggcatcgt
     2161 cgccaacaac ggcatcctgt tctcggaatc ggcgttgaaa ggcacgcact tcattgagct
     2221 ctgcagccag cgcgggattc cgctcgtgtt cctgcagaac atcaacggct tcatggtcgg
     2281 caaaaaatac gaggcaggcg gcatcgccaa ggacggcgcg aaaatggtga cggcggtcgc
     2341 ctgttcgcgc gtgccgaaat tgaccgtgat cgtcggcggc agcttcgggg cgggcaacta
     2401 cggcatgtgc ggccgcgcgt tcgatccacg ctttgtctgg atgtggccca atgcgcgaat
     2461 ttccgtaatg ggcggcgagc aggcggccaa tgtgctcgcg caggtcaagc gcgacgcgct
     2521 cgagaaagcc gggcagacgt ggagcgccgg cgatgaaaac gatttcaagg cgcctatcct
     2581 cgagcagtac gcgcaacag
//
LOCUS       DATANS010000187         2128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_179041, whole genome shotgun sequence.
ACCESSION   DATANS010000187 DATANS010000000
VERSION     DATANS010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179041"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VHT22_02705"
     CDS             complement(<1..638)
                     /locus_tag="VHT22_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136492.1"
                     /translation="MALLIGVTAWAAAPTVPGSPLTAQSQVSGITAPPIRLEVEPAPE
                     QVAQAPAQTPMPVPTPQPQVPATAQEAPAAPPISEDEQQALALLADLQRYATAPADEL
                     RRELASVNQASNPRVRTDVNRIRLAVLLTMPNAGPPDDGRALALLESVAGKTGGASPL
                     KQLAMVLYAQVAERVRNVAEERKRSALAQEKLDQLRAVERSLLLERSRNAGGA"
     gene            complement(674..>2128)
                     /locus_tag="VHT22_02710"
     CDS             complement(674..>2128)
                     /locus_tag="VHT22_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3136493.1"
                     /translation="LLGFLLVSLPLLYALVELIVSLDRLGTQSQQAVLQAAQAGRASR
                     QLYEQATTLERLVRQQLILDDPQLIDDYGRLRQDFRQTSQQLMQLPLDRTQIAQLEKI
                     ADDETRLFSLLKTPQRPPGAAKTLADGYATLAEDTQSMISATNRLTEHEIQRLQQTAA
                     EGSRTWGYLALAAGLIAMLLAITFAVLIARPIRQLDQAIRQMGSADFTHAIEVNGPQD
                     MRYLGQRLEWLRSRLHELEEQQNRFLRHVSHELKTPLTAVREGAELLRDRVGGELSPE
                     QREIVRIVRENTLSLQKLIEDLLNYHQTRSIEPQTLGPVALADVVRRVVREHKLAAFA
                     RLINVETKLVPSMIVGDGEKVRAIVDNLLSNAIKYSPRSGTIAIELRPEGDQAVLDVI
                     DGGPGISAADRPHIFESFYQGKTAPQGRIKGSGLGLAIAREYTLAHGGRIELIDRTDG
                     RRGAHFRLYLPLAMPGQRALAKGAKPIITLQEEG"
BASE COUNT          313 a          710 c          711 g          394 t
ORIGIN      
        1 gcgccgcctg cgttgcgact tcgctcgagc agcaggcttc gttcgaccgc ccgcagttga
       61 tcgagttttt cctgggcgag cgcagagcgc tttctttcct ccgcaacatt gcgcacgcgt
      121 tcggcgacct gggcgtaaag caccatcgcg agttgtttca gcggcgatgc gccgccggtt
      181 ttgccggcaa ccgattcgag cagggccagc gcgcggccat cgtcgggtgg acccgcattg
      241 ggcatcgtca gcagcaccgc cagtctgatc cggttgacat cggtgcgcac gcgcgggttg
      301 cttgcctgat tcaccgacgc gagctcgcgc cgcagctcat cggcgggagc ggtggcatag
      361 cgctgcaggt cggcgagcag ggccagcgcc tgctgctcgt cctcgctgat cggcggtgcc
      421 gccggtgctt cctgcgcggt ggccggcacc tggggttgcg gcgtcggaac cggcatcggc
      481 gtctgtgccg gcgcctgcgc gacctgctcc ggcgccggct cgacttcgag gcgaatcggc
      541 ggcgccgtga tgccggagac ctgcgactgc gccgtcagcg gcgaaccggg caccgtcggt
      601 gccgccgccc atgcagtcac accgatcagc aatgccattg tcgcgccggc gcgggcagca
      661 tcctgccgaa ggctcaccct tcctcctgga gggtgataat gggttttgcg cccttggcca
      721 gcgcgcgttg tcctggcatc gccagcggca ggtacaggcg gaaatgcgcg ccgcgccgcc
      781 cgtcggtgcg atcgatcagc tcgatgcggc cgccatgagc cagcgtgtat tcgcgtgcga
      841 tcgcgaggcc cagccccgag cccttgatcc ggccctgggg cgcggtcttg ccctggtaga
      901 acgattcgaa gatgtgcggc cgatcggccg cactgatgcc cgggcctcca tcgatgacgt
      961 cgagcactgc ctggtcgccc tccggccgca actcgatcgc gatcgtgccg gagcgcggcg
     1021 aatacttgat cgcgttcgac agcagattgt cgacgatggc gcgaaccttt tcgccgtcac
     1081 cgacgatcat cgaaggaacg agcttcgtct ccacgttgat cagccgcgcg aatgcggcca
     1141 gcttgtgctc gcgcacgacg cgccgcacca catcggccag tgcaaccggc cccagtgttt
     1201 gcggctcgat gctgcgggtc tggtgatagt tgagcagatc ctcgatcagc ttctggagcg
     1261 acagcgtgtt ctcgcgcacg atgcgcacga tttccctctg ctcgggcgag agctcgccgc
     1321 cgactctgtc gcgcagcagc tcggcgcctt cgcgcaccgc ggtcagtggg gtcttgagct
     1381 cgtgcgagac gtgccgcaga aagcgattct gctgctcctc gagctcgtgc aggcgcgagc
     1441 gcagccattc caggcgctgc cccagatagc gcatgtcctg cgggccgttg acctcgatcg
     1501 cgtgcgtgaa atcggcgctt cccatctggc ggatggcctg gtccagctgc cgtatcggcc
     1561 gtgcgatcag gacggcgaag gtgatcgcca gcaacatggc gatcaatccg gcggcgagcg
     1621 ccaggtagcc ccaggtcctg cttccttccg ccgccgtctg ctgcagccgc tggatctcgt
     1681 gctcggtcag ccggttggtg gcgctgatca tcgattgcgt atcctcggcc agcgtcgcat
     1741 agccgtcggc aagcgttttc gccgcacccg gcggacgctg tggcgtcttc agcagcgaaa
     1801 agagccgggt ttcgtcgtcg gcaattttct cgagctgcgc gatctgcgtg cgatcgagcg
     1861 gaagctgcat cagctgctgc gacgtctgcc ggaaatcctg ccgcaggcgc ccgtaatcgt
     1921 cgatcagttg aggatcgtcg agaatcaact gctggcggac caggcgctcg agcgtcgtcg
     1981 cctgctcata aagctggcgg ctggcgcggc cggcttgcgc tgcctgcagc accgcttgct
     2041 gactctgcgt cccgagtcgg tcgaggctga cgatcagttc gaccagcgca tacagcaagg
     2101 gcagacttac cagcaggaag ccgagcag
//
LOCUS       DATANS010000188         2261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_219593, whole genome shotgun sequence.
ACCESSION   DATANS010000188 DATANS010000000
VERSION     DATANS010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219593"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..356
                     /locus_tag="VHT22_02715"
     CDS             30..356
                     /locus_tag="VHT22_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136494.1"
                     /translation="MEESQVRSTPYNPKIENAAQQAHQNVDRVADKAVSGVGRASDSV
                     HRAVNTTADKAASAAEWASQVPEKARRAQGAMTEAACDTIRARPLTAVFSALAAGYVL
                     GRIARW"
     gene            complement(419..1321)
                     /locus_tag="VHT22_02720"
     CDS             complement(419..1321)
                     /locus_tag="VHT22_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX3136495.1"
                     /translation="MPLFHFDFDAMASPNELQIWHDDESRARTLADTAIADVRRIEAK
                     YSRYRDDSVTARINRAAGGAAVAIDAETYALLGYADHCHRLSAGRFDITSGVLRRAWD
                     FRRSPPRLPDQDEIAAAIALIDWPCVEWSRRSVRLPRPGMEIDFGGIGKEYAADRMAT
                     ICHDGGAPHALANLGGDVRAAGPQQDGSPWRVGISHPRQADAVMATVKIDGGAVATSG
                     DYQRFFDAGGRRYCHIIDAHSGQPVTRWQSVSVIAPLCVLAGSCATIAMLFEGEAEAF
                     LAREGVEYLAVDSAGVLHKSAAPA"
     gene            complement(1370..>2261)
                     /locus_tag="VHT22_02725"
     CDS             complement(1370..>2261)
                     /locus_tag="VHT22_02725"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HEX3136496.1"
                     /translation="SLVYDGISGASPLHHNTLSGASGKGGVTDYRTAGDVKLTKYFDG
                     VAVGVGAAISSERDYLSRALSLDVRVSSDDRNRTYAFGIGGASDDINSTNGIAEGMHR
                     RTVDLLLGVTQALSPEAIVQSNLTWSAGHGYYSDPYKTLDTRPNGRRILAWLTRYNQH
                     LSPTDATLSLSYRYARDSFGSDSHTVEAAWHQPLPDGWSIKPSLRYYTQAAADFYFDP
                     PFPQGFSLGEPYTADTRLSAFGVLNPGLTVAKLLPGGWQIDVKVEFYRQRSDWRPGGG
                     SPGLEPLSARWIQTGISKPF"
BASE COUNT          395 a          733 c          771 g          362 t
ORIGIN      
        1 attagacttt gaccgacgag gagatagtga tggaagaaag ccaagttcga tccacccctt
       61 acaatccgaa gatcgagaat gccgctcagc aggcgcacca gaacgtcgac cgtgttgccg
      121 acaaggccgt atccggcgtc ggccgggcca gcgactcggt gcaccgcgcc gtcaacacca
      181 ccgcggacaa ggcggcatcc gccgcggagt gggcgagcca ggtgccggag aaggcccggc
      241 gtgcgcaagg cgcgatgacc gaggcggcat gcgatacgat ccgggcgcgg ccgctgacgg
      301 cggtattcag cgcgctcgcg gctggttacg tgctcggacg aatcgcccgc tggtaggggt
      361 cggctcggcc cgcgacggtg gagcaacggc gctgcatact cagcgccgtt cgcgtgtttc
      421 aggcaggcgc cgcagacttg tgcaatacgc cggcagagtc gacggcgaga tactcaacgc
      481 cctcgcgtgc aaggaaagcc tcggcctcgc cctcgaaaag catcgcaatc gtcgcgcagc
      541 ttccggcaag cacgcatagc ggcgcgatga cgctgaccga ctgccagcgc gtgaccggct
      601 gcccggagtg ggcgtcgatg atgtggcaat agcggcgtcc gccggcgtcg aaaaaccgct
      661 ggtagtcgcc gctggtcgcc accgctccgc cgtcgatttt caccgtcgcc atcaccgcgt
      721 cggcttgacg cggatgcgaa atgcccacgc gccaaggcga tccgtcctgc tgcggacccg
      781 cggcgcggac gtcgccgccg aggttggcga gcgcgtgcgg cgcgccgccg tcgtggcaga
      841 tcgtcgccat cctgtccgcc gcgtattcct tgccgattcc gccgaaatcg atctccatgc
      901 cgggtcgcgg caggcgcacg ctccggcgac tccattcgac gcaaggccag tcgatcagcg
      961 cgatcgccgc cgcgatttcg tcctggtcgg gcagtcgcgg cggtgaacgg cgaaaatccc
     1021 acgcgcggcg aagcacgccg gaggtgatat cgaagcggcc ggcgctcagg cgatggcagt
     1081 gatcggcata gcccagcaag gcgtaagttt cggcgtcgat cgcgaccgcc gcgccgccgg
     1141 cggcgcgatt gatccgcgcg gtcacgctgt cgtcgcgata gcgcgaatac ttcgcctcga
     1201 tgcgccgcac gtcggctatc gcggtatcgg ccagcgtgcg cgcgcggctc tcgtcgtcgt
     1261 gccagatctg cagctcgttc ggcgaggcca tcgcgtcaaa gtcgaagtgg aacagcggca
     1321 tcgcgtaact atgcccgggg cttggcggcc gagatcgaat cgcgccgctt tagaatggct
     1381 tgctgatgcc cgtctggatc cagcgcgccg acagcggctc gaggccgggg ctgccgccgc
     1441 cgggccgcca gtccgagcgc tgccggtaga actcgacttt gacatcgatt tgccagccgc
     1501 cgggcagcag cttggcgaca gtgagtcccg ggttcagcac gccgaacgcc gacagccgtg
     1561 tgtccgccgt atacggctcg ccaagcgaaa atccctgcgg gaacggcggg tcgaaataga
     1621 aatccgccgc ggcctgtgtg tagtagcgca agctgggttt tatcgaccag ccgtccggta
     1681 acggctgatg ccacgccgcc tcgaccgtgt gcgaatcgct gccgaaagaa tcgcgcgcgt
     1741 aacggtaaga caacgacagc gtcgcatcgg tcggggacaa atgctggttg taccgcgtga
     1801 gccacgcgag gatgcgcctg ccgttgggac gggtgtcgag cgtcttgtac gggtcggaat
     1861 agtaaccgtg ccctgccgac caagtcaggt tcgactgaac gatcgcttcg ggcgacagcg
     1921 cctgggttac gccgagcagc aggtcgacgg tccgcctgtg catgccttcg gcgatgccgt
     1981 tcgtgctgtt aatatcgtcg ctcgcgccac cgatgccgaa tgcgtaggtg cggttgcggt
     2041 cgtcgctcga tacgcgtacg tccagcgaaa gcgcgcgaga aagataatcg cgttcggacg
     2101 aaatcgcggc gccgacgccg accgcgacgc cgtcaaagta cttggtcagc ttgacgtcgc
     2161 ccgcagtgcg gtagtcggtc acgccgccct tgcccgacgc gccggaaagc gtgttgtgat
     2221 gcagcgggga cgcgcccgag atgccgtcgt agaccagcga g
//
LOCUS       DATANS010000189         1784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_640747, whole genome shotgun sequence.
ACCESSION   DATANS010000189 DATANS010000000
VERSION     DATANS010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640747"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1254)
                     /gene="rimO"
                     /locus_tag="VHT22_02730"
                     /pseudo
     CDS             complement(1..1254)
                     /gene="rimO"
                     /locus_tag="VHT22_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
     gene            complement(1349..>1784)
                     /locus_tag="VHT22_02735"
     CDS             complement(1349..>1784)
                     /locus_tag="VHT22_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PHB accumulation regulatory domain-containing
                     protein"
                     /protein_id="HEX3136497.1"
                     /translation="VPMFSSDVLSKIIRFYGNAMQGMMGNYLEKNIQTFIEIQKKLQA
                     QSSALYGGGTAMPNAEAWSEFLKMQGPAIQGLMGSYLEQSANAFLEMQQQMQKQSRNL
                     FGNFPFPNFAAANPFAPPPREDAGQTASSPAPKDEDGDKKGG"
BASE COUNT          279 a          559 c          577 g          369 t
ORIGIN      
        1 gacgttcgca aattcgcccg cgacgagctt cggcgacggc gcgaggtgga cgacgccgtc
       61 gatttcaggc gcatcggcgc tgctgcgtgc gacgaccgcc ttggcatcgg catgatcgat
      121 cagcacgcgc tgcgtcgtgc cgactttgcg ctggagccgc gcagtgctga cgagcgcctg
      181 cgccgccatg aaccgcgcct tgcgttcttc tttcaattct tccggcacgt ggttcggcag
      241 cgcgttggcg gccgcgccgt cgacagctga atacgtgaag cagccgacac ggtcgagttg
      301 cgcgtcctcg atgaactcga gcagctgctc gaactcctcg tcggtttcgc cgggaaagcc
      361 gacgatgaac gtgctgcgga tcgtcaggtc ggggcagatt tcgcgccagc gccggatgcg
      421 cgcgaggttg ttttccgaat tggcgggccg cttcatcgct ttcaggatgc gcgggctcgc
      481 gtgctggaat ggcacgtcga ggtaaggcag gatttttccc gcgcgcataa gcggcagcac
      541 ttcgtcgacg tgcggatacg gatagacgta atgcaggcgc acccacgcgc cgagctcccc
      601 cagcgcaacc gcgagctccg tcatgcgcgt cttgagcggc cgcccgcccc agaagccggt
      661 gcggtacttg acgtcgacgc cgtacgcgct ggtgtcctgc gaaatgacga gcaactcttt
      721 gacgcccgcc ttgaccaggt tttccgcctc ctgcatgact tcgccgacgg gacggctgac
      781 caaggcgccg cgcatcgacg ggatgatgca gaacgtgcag cggtggttgc agccctcggc
      841 gattttcaga taggcgtaat gccgcggcgt gagtttgatt ccctgcggcg ggatcaagtc
      901 gatgtgcgga tcgggcggct gcggcaggtg ctcgtgcacc gcgcgcatga cttcatcggt
      961 cgcatgcggc ccggtgaccg cgagcacgcg cgggtgcgca tcctgcacca cgctgccctt
     1021 ggcgccgaga cagcccgtaa cgatgactgt gccgttttca acgagcgctt caccgatggc
     1081 gtcgagcgat tccgcgaccg cgctgtcgat gaagccgcag gtgttgacca ccacgaggtc
     1141 cgcatcctga tagctcggcg cgacgagata accctccgcg cgcagctgcg tcaagatgcg
     1201 ttcggaatcg accagtgctt tggggcagcc caatgaaacg aaaccgactt ttggcgccgc
     1261 gccgcggcgc ttgcgcggct ttgccggcgt aactcttgtt gccgtccctt gctttttagg
     1321 cgcagtttct gtcgccatcc gctgcttttc agccgccttt tttgtcgccg tcttcatcct
     1381 tgggcgcggg tgagcttgcg gtttggccgg cgtcttcgcg cggcggcggc gcgaacggat
     1441 tggcagcggc gaaattcggg aacgggaagt tgccgaacag gttgcgcgac tgcttctgca
     1501 tctgctgctg catttcgagg aacgcgttgg cgctctgctc gagatagctg cccatcaggc
     1561 cctggatcgc cgggccctgc attttcagaa attcgctcca cgcttccgcg ttcggcatcg
     1621 ccgtgccgcc gccgtagagc gcgctcgact gcgcctgcag ctttttctgg atttcgatga
     1681 acgtctggat gttcttttcg aggtaattgc ccatcatgcc ctgcattgcg ttgccgtaga
     1741 accggatgat cttcgacagc acgtcggagg agaacatcgg aacg
//
LOCUS       DATANS010000190         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_724285, whole genome shotgun sequence.
ACCESSION   DATANS010000190 DATANS010000000
VERSION     DATANS010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724285"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="VHT22_02740"
     CDS             complement(<1..143)
                     /locus_tag="VHT22_02740"
                     /inference="COORDINATES: protein motif:HMM:NF016179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEX3136498.1"
                     /translation="MLLIPCPWCGPRNQIEFTYGGDASLKRPPAEAPVEAWFEFVYLR
                     DNP"
     gene            complement(166..1419)
                     /locus_tag="VHT22_02745"
     CDS             complement(166..1419)
                     /locus_tag="VHT22_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HEX3136499.1"
                     /translation="MRYSIFSLARNAASRHENWPLAWRSPEPKPSYDAIVVGGGGHGL
                     ATAYYLAKEHGISNVAVLEKGWLGGGNTGRNTTIVRSNYHLDANEHFYEFSLKLWESL
                     SQELNFNVMYSARGVLNLVHSPADMDAAIRRGNAMRLNGIDAKFLSRDEIAAWIPELD
                     TSPTARFPIVGGLLQPRGGTVRHDAVAWGYARGADARGVDIIQNCEVTGIRIEGGAIA
                     GVDTTRGFIATRKLGIAVAGNSSRLAAMAGIRLPIESHVLQAMVSEPIKPVLDTVVSS
                     GVVHFYISQSDKGELVMGGDLDFYNSYAQRGNLPALEHVVAGCLALFPSFGRLRQLRS
                     WGGIMDMTMDGSPIIARLPVDGLTMTGGWCYGGFKATPASGWLHAYTMARGEPHPLNA
                     AFTLERFAEGRMIDEKGAGPRPGAH"
     gene            complement(1467..2297)
                     /locus_tag="VHT22_02750"
     CDS             complement(1467..2297)
                     /locus_tag="VHT22_02750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3136500.1"
                     /translation="MEDLNPQAEQMADESMVRNLAAQAQAIWPQEAPLFSRYALPADA
                     RILDAGCGTGEAASRLAGLFPRATILGVDIIDAHLELARTRYRSLAARLSFEHQSVYK
                     LAAADHSFDLTVCRHVIHAIPYPERVLAELKRVTRPGGYLHLIPEDYGMLHFQRGALD
                     PQDFWHIVPARFGAATGTDLFIGRNVFGILAAMNVETITVDYVVVDTVRVPRQTFASI
                     LEAWRDGYVEPIAELTPITREQALAYFEQMIADIRNPHRYAAWMVPVVSARVPGALAR
                     "
BASE COUNT          419 a          747 c          795 g          407 t
ORIGIN      
        1 cagggattgt cgcgcagata gacgaattcg aaccaagcct cgacaggcgc ttccgccggc
       61 gggcgcttga gggacgcgtc gccgccgtag gtgaattcga tctggttgcg cggaccgcac
      121 catggacagg gaatcagcag catcgcgagt gctctctccc gtccatcaat gcgcacctgg
      181 gcgcggcccg gcgccttttt cgtcgatcat ccggccttcg gcgaagcgct ccagcgtgaa
      241 tgcggcattc aacgggtgcg gctcgccgcg cgccatcgtg taggcgtgca gccaacctga
      301 cgccggtgtt gccttgaacc caccgtagca ccatccgccg gtcatcgtca ggccatccac
      361 cggaagcctc gcgatgatcg ggcttccgtc catcgtcatg tccatgatcc cgccccagct
      421 ccgcagctgg cgaagacgtc cgaagctcgg aaacagcgcg agacatcctg cgaccacatg
      481 ctcgagcgcc ggcaggttgc cgcgctgggc gtaggaattg tagaagtcga ggtcgccacc
      541 catgaccagc tcgcccttgt ccgactggct gatgtagaag tgcacgacac ccgacgacac
      601 gacggtgtcg agcactggct tgatcggctc ggacaccatc gcctgcagca cgtgcgattc
      661 gatcggcaga cggatcccgg ccatcgcggc caggcggctc gagtttccgg cgaccgcgat
      721 ccccagcttg cgggtcgcaa tgaagccgcg ggtcgtatcg acaccagcga tggcgccgcc
      781 ctcgatgcgg atgccggtaa cctcgcagtt ctggatgatg tcgacgccgc gtgcgtcagc
      841 gcctctcgcg tagccccagg ctacggcgtc gtggcgcacc gtgccgccgc gcggttgcag
      901 caatcctccg acgatgggaa agcgcgctgt gggtgaagta tcgagctcgg gaatccaggc
      961 tgcgatttcg tcgcggctca agaatttggc atcgatgccg ttcaaacgca tcgcgttgcc
     1021 gcggcggatc gccgcgtcca tgtctgcggg agagtgcacg aggttcagca cgccgcgtgc
     1081 gctgtacatg acgttgaagt tgagctcctg cgacaggctc tcccagagct tcagcgagaa
     1141 ttcgtagaaa tgctcgtttg catcgaggtg atagttcgag cgcacgatgg tcgtgttgcg
     1201 gccggtgttg ccgccgccga gccagccctt ttccagcacg gccacgttgc tgataccgtg
     1261 ctctttggcg aggtagtagg cggtggccag gccgtggccg ccaccgccga cgacgatcgc
     1321 gtcgtagctg ggcttcggct cggggctgcg ccaggcgagc ggccagtttt cgtgacggct
     1381 cgccgcgttt cgggcgagcg aaaagatcga gtagcgcatc ggcggctatc gaactcgacc
     1441 gggacctgcg gcggacgcgg acgcgatcat cgggccagcg ctcccggcac ccgcgcgctc
     1501 accaccggga ccatccatgc ggcgtagcga tgcggattgc ggatatcggc gatcatctgc
     1561 tcgaaatagg cgagcgcctg ctcgcgcgta atcggggtca attcggcgat cggctcgaca
     1621 tagccgtcgc gccaggcttc gaggatggag gcaaacgtct gccgcggcac gcgcaccgta
     1681 tcgacgacga cgtagtcgac ggtgatggtc tcgacgttca tcgcggcgag aattccaaag
     1741 acgttgcggc cgatgaaaag atcggttccg gtcgcggcgc cgaatcgtgc cggaacgatg
     1801 tgccagaagt cctgcggatc gagcgcgccg cgctggaaat gcagcatccc gtagtcttcc
     1861 ggaatcaggt gcagatagcc gccgggccgg gtcacccgct tgagctcggc gagcacacgc
     1921 tccggatacg gaatcgcgtg gatgacgtgc cggcagacgg tgaggtcgaa gctgtgatcg
     1981 gcggcagcga gcttgtagac gctctggtgc tcgaatgaga gccgggcggc gagacttcgg
     2041 tagcgcgtgc gggccagctc cagatgcgca tcgatgatgt cgacgccgag aatggtggca
     2101 cgcgggaaaa gcccggcgag ccgggaggcg gcctcgccgg tgccgcagcc ggcatccaga
     2161 atgcgggcgt cggcgggcag cgcataacgg ctgaaaagcg gcgcttcctg cggccagatc
     2221 gcttgcgcct gcgcggccag attccgcacc atcgactcgt cggccatttg ttcggcctga
     2281 gggttgagat cctccaacga ctactcctag cttgccaaag cgggaacgga aggctggcgc
     2341 gccgctaaag cgcacaatcc aagtcgta
//
LOCUS       DATANS010000191         1596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_9338, whole genome shotgun sequence.
ACCESSION   DATANS010000191 DATANS010000000
VERSION     DATANS010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9338"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VHT22_02755"
     CDS             complement(<1..298)
                     /locus_tag="VHT22_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3136501.1"
                     /translation="MDIERALVKTSMGYIHYRAAGRGAPLVLLHMNQQSSALYLELMA
                     VLAPGVRAIAIDYPSHGMSDHVTIQPTIADYARWVLEVVDALELKTASFLGESGG"
     gene            421..1296
                     /locus_tag="VHT22_02760"
     CDS             421..1296
                     /locus_tag="VHT22_02760"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3136502.1"
                     /translation="MTTAELTTYAIDVTDIEYLRHGDKPLLARLYKPRGTGPFPIIVD
                     LHGGAWCKKDRMSDAGTDEPLAKSGVIVVALDFRMPPDAGYPASLADVNYAIRWCKAH
                     AAEWNGRADRVGVLGVSSGGHQAMLAAMRPADMRYAALPLAGKSAVDAKVHCAVMCWP
                     VIDPLGRYQYAKAAQKGNDEKLKKSADEWVICHDKYWGSEAAMTEGNPTLALERGEKT
                     DLPPVLYLQGTADIAHPKPNRDQFIASYRKAGGRVDLHLFEGVGEAFITNDPTSQAAR
                     DAIERIVEFVHREMQ"
     gene            complement(1298..>1596)
                     /locus_tag="VHT22_02765"
     CDS             complement(1298..>1596)
                     /locus_tag="VHT22_02765"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX3136503.1"
                     /translation="ATRSPALPDVPTVAEAGLPGFEFDTWYGLFAPAKTPAQVVRQIS
                     AEVARILKLPDVKERFDGRGAVARPSTPEEFNQFVRAEFVKLGAVVKAANLKVD"
BASE COUNT          300 a          496 c          529 g          271 t
ORIGIN      
        1 cgccgccgga ttcgccgagg aagctcgcgg ttttcaattc cagcgcatcc acgacttcga
       61 gcacccagcg cgcgtagtcg gcgatggtcg gctggatcgt aacgtggtcc gacatgccgt
      121 ggctcggata gtcgatcgcg atggcgcgca cgccgggtgc cagcaccgcc atcagttcga
      181 gatacagcgc cgacgattgc tggttcatat gcagcagcac gagcggcgcg ccgcggccgg
      241 cggcacggta atggatgtag cccatgctcg ttttcacgag cgcgcgttcg atgtccatgg
      301 cggggtcccg gggaaggcag atggagtgcg ctgattttcg catgtattac acggtccggc
      361 gctgacgcgc ccggcgctac gatttgccgt aaaatcaggt gcacttcaca ggaggacttt
      421 atgaccaccg ccgaactcac cacctacgcc atcgacgtca ccgatatcga atatttgcgc
      481 cacggcgaca agccgttgct cgctcgcctg tacaagccgc gcggcacggg cccgttccct
      541 ataatcgtcg atctgcatgg cggcgcgtgg tgcaagaaag atcgcatgag cgatgccggc
      601 accgacgagc cgctcgcgaa aagcggggta attgtggtgg cgctcgattt ccgcatgccg
      661 cccgacgcgg gctatcccgc atcgctcgcg gacgtcaatt acgcaatacg ctggtgcaaa
      721 gcgcacgcgg cggagtggaa cggccgcgcc gaccgcgtgg gcgtgctcgg cgtctccagc
      781 ggcggtcacc aggcgatgct cgcggcgatg cggcccgcgg atatgcgtta tgccgcgctg
      841 ccgctggcgg gaaaatccgc ggtcgacgcg aaggtccact gcgcggtgat gtgctggccg
      901 gtgatcgatc cgctcggccg ctatcagtat gcgaaggccg cgcagaaagg caatgacgag
      961 aagctgaaaa aatccgcgga cgagtgggtg atctgtcacg acaagtactg gggcagcgaa
     1021 gcggcgatga ccgagggcaa tccgacgctc gcgctcgagc gtggcgaaaa gaccgacctg
     1081 ccgcccgtgc tgtacctgca gggcaccgcc gacatcgcgc atccgaaacc caatcgcgac
     1141 cagttcatcg ccagctatcg caaggcaggc gggcgcgtgg acctgcatct attcgaaggc
     1201 gtcggcgagg ctttcatcac caacgatccg acttcgcagg cggcacgtga cgcgatcgag
     1261 cgcatagtcg aattcgtgca tcgcgaaatg cagtgaacta gtcgactttc aggttggcgg
     1321 ccttgacgac cgcgccgagc ttgacgaact cggcgcgcac gaactggttg aattcttcag
     1381 gcgtcgacgg ccgcgcgacg gcgccgcgcc cgtcaaagcg ctctttcacg tccggcagct
     1441 tcagtatgcg cgccacctcg gcggagattt gcctgacgac ctgagcgggc gttttcgcgg
     1501 gggcgaagag tccgtaccac gtatcgaatt cgaatccggg cagtccggct tcggcaacgg
     1561 tcggcacatc gggcagcgca ggcgagcggg tggcgg
//
LOCUS       DATANS010000192         4327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_9344, whole genome shotgun sequence.
ACCESSION   DATANS010000192 DATANS010000000
VERSION     DATANS010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9344"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VHT22_02770"
     CDS             <1..374
                     /locus_tag="VHT22_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3136504.1"
                     /translation="RVLVVDDNRDSADTLAALLEAWGHDVRTLYDGPSAIAAVAEFKP
                     NVVLLDIGLPKMNGYEVAAQLRQLANRRPLILVAFTGYGQDEDRRRVREAGFDYHLVK
                     PLEPAELEKILDSVTVDAAAA"
     gene            complement(477..878)
                     /locus_tag="VHT22_02775"
     CDS             complement(477..878)
                     /locus_tag="VHT22_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3136505.1"
                     /translation="MQLIPYLVFNGQCEAAFKFYAECLGGKIEAMMPHRGTPVEQHVS
                     PDWQDKILHARLNTGDAVLMGSDAPPRRYDEPRGFSVSLQVKDPAEAERIFRALAEKG
                     SVRMPLDKTFWAQRFGMLVDRFGIPWMVNCE"
     gene            1189..1578
                     /locus_tag="VHT22_02780"
     CDS             1189..1578
                     /locus_tag="VHT22_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3136506.1"
                     /translation="MADAPGALHVYMIEDSPIFSRLLAAAVESVGGELVGHSDNAEQA
                     IADLSRVDADLILVDVTLESGSGFEVLEALQKSGKARTATKVVLTNHTAAECGGLSYS
                     LGATYFYEKEHDSWRVLELISKMAAGK"
     gene            1864..4326
                     /locus_tag="VHT22_02785"
     CDS             1864..4326
                     /locus_tag="VHT22_02785"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HEX3136507.1"
                     /translation="MADLASTQSSPSPATDVDGNLLLSSEDAQALLRECFAEFKAGLI
                     ALVHVSIETTNDLFEGNAFIGEAEVADFRSKRGQWIERFGQALTDLFERRLAGTRRQG
                     RRPDFDASLTTLRVLNTFDHEKQASLTSSTQFLYRLTKRELDAIDLRVGALLGEPRMR
                     DVDNPFSPDYVLDAIGLSSRELYPNPRVWRPLMERLLSDVTLGINKSFIKVNRFLADH
                     QVLPEIKAQLRARSELRPRDDAELLPTFLRLFKEGRSAAADQLLALGIAVPAASASTS
                     TALKDLPKPAPAHAAPASTPGGDSSQAESAPPAPSAAPAPPTIDTGPGAALSVALNPY
                     IADLARALQSSEAATAPATGMSGLPQVDPLMAIGSLSAAVAVLDRWQRLDPGSDHRAA
                     EAASGDAASRAAAMNRIPFIRAAIDDKVTSSTDKITMDVIGLLFDYIFRDPSIPEDLR
                     SLFTRLQVPILKTALLDRSFFSDRKHPARRLLDHLAGAAIGATGDAGYRAAFELTAAG
                     VIEEVCRDFQVDVGVFDLADRKVQEFVDAELQKGAGALGTEVAAALAAEEGEADRSAV
                     RALIRDKLSGLDVPFDVRSFSETIWADYLTNVRLAQGSEGDEWRRGLRTLDDLLWSLT
                     AKERSAQKARLAKLVPGMIRNLRAGAAAVNVGDERVKPLLDAMYQLHMAAIKPASADE
                     VHAPAAAAAGAGAGKAEAGAGKAEAGAPVEAVAPAPKIANVHDFVAEMVVGTWLAFGR
                     GDKTVNARLSWISPMRSKYLFTTRERSRAIVATPEDLAWQLGAGKATLIVEPVPLFDR
                     AVSAALDTLAAQKPRGDTAAAA"
BASE COUNT          717 a         1446 c         1426 g          738 t
ORIGIN      
        1 tgcgcgtgct cgtcgtcgac gacaatcgcg attccgccga tacgctggcg gcactgctcg
       61 aagcctgggg gcacgacgtc agaacgcttt acgacggacc gtccgcgatc gccgccgtgg
      121 cggagttcaa gccgaacgtc gtgctgctcg atatcggcct gccaaaaatg aatggatacg
      181 aagtggccgc gcagctgcga caactggcga accggcggcc gttgatcctg gttgcattta
      241 ccggctacgg acaggacgag gatcgccgtc gcgtgcgcga agcaggattc gactatcacc
      301 tggtcaagcc gctcgagccg gcggagctcg agaagattct cgactcggtc accgtcgacg
      361 ccgcggccgc ctagggacgc ggcggtcgcc gagggtcacg gccggcgatt gcgccgggca
      421 catcggcccc cagcgagcga accggccgcc ccggcgccgg cccacgcgcg cgcgagctat
      481 tcgcagttga ccatccaagg gatgccgaag cgatcgacca gcatcccgaa gcgttgcgcc
      541 cagaatgttt tgtccagcgg catgcgcacg ctgccctttt cggccagcgc gcgaaaaatt
      601 cgctccgcct cggccgggtc cttgacctgg agcgacaccg agaaccccct gggctcgtcg
      661 tagcgtcgcg gcggcgcatc ggaacccatc agcacggcgt ccccggtgtt tagccgagcg
      721 tgcaggattt tgtcctgcca atccggcgag acatgctgct ccaccggtgt gccgcggtgg
      781 ggcatcattg cctcgatctt gccgcccagg cattcggcgt agaacttgaa cgccgcttcg
      841 cattgtccgt tgaacacgag ataaggaatc aattgcacgg tgcgctcctt ttgtccgtcg
      901 gtagggtact cggccatgta cgaccctcgt ggccgccggc agttgccaac tgtcagcaaa
      961 tgcctgagcg atagccgggt ccccctcgac ttttcgcgcg ctcgctgccc ggcccggcgg
     1021 gacttctcgc cggttgcgcc gggaccggga catgattttc agcaacagtc cgacttattt
     1081 cgtccgatat tcctacatgt attcccgctg cgcggcgtga tactgccgac aggggtcgct
     1141 tcgcttttcg cgcgggcggc gcatgctggc aatgggaaca cgacggatat ggccgacgct
     1201 ccaggcgcgc ttcacgtcta catgatcgag gactcgccga tcttctcgcg gctgctggca
     1261 gcggccgtcg agtcggtggg aggcgagctg gtcggccaca gcgacaacgc cgaacaggcg
     1321 atcgccgatt tgtcgcgcgt cgatgccgac ttgattctgg tcgacgtgac gctggaatcg
     1381 ggcagcggct tcgaggtgct cgaggcactg caaaagagcg gcaaggcccg aaccgcgacc
     1441 aaggtcgtcc tgaccaacca taccgctgcc gaatgcggcg gtttgagcta ttcgctgggc
     1501 gccacgtact tctatgaaaa ggagcacgat agctggcgcg tgctcgagtt gatcagcaag
     1561 atggccgcgg gcaaatagag cctcgcgcgc cggcgccggc ggcgcctgca gcatacaatt
     1621 gtggatcgct gtcgcggcat gaatgcaggt cgccgatgcc gcgaacctcc ttccaggccg
     1681 tctgttcgcc ttcgatcccc ggcacggtga cgatcgtcgc ggcgcgctcg ccaaagcggg
     1741 caaaggccgt tagcattcga ccaccctagc gaagggcgct gttcgggcga agcgggccta
     1801 tgacggcacg gccggctttt gaattcgggc tgcgttcgtt gcccggattt cgcccagacg
     1861 accatggccg acctcgcctc gacacagtct tccccgtcgc ctgcaaccga cgtcgacggc
     1921 aatctgctgc tgtccagcga agacgcgcag gcgctgctgc gtgaatgctt tgccgagttc
     1981 aaagccgggt tgatcgcgct ggtccacgtc tcgatcgaga ccaccaacga tctgttcgag
     2041 ggcaacgcat ttatcggcga ggccgaggtc gccgatttcc gcagcaagcg cgggcaatgg
     2101 atcgagcgct tcggccaggc gctgaccgac ctgttcgagc ggcggctcgc cggcacgcgt
     2161 cggcaggggc gccggcccga tttcgatgcc tcgttgacga cgttgcgcgt gctcaatacc
     2221 ttcgatcacg aaaagcaggc gtcgctgacg tcgagcacgc aattccttta ccggctgacc
     2281 aagcgcgagc tcgatgcaat cgacctgcgc gtcggcgcgc tgctcggcga gccgcgaatg
     2341 cgcgacgtcg acaatccgtt ttccccggac tacgtgctcg acgcgatcgg actgtcttcc
     2401 cgcgagctgt acccgaaccc gcgcgtgtgg cggccgctga tggagaggct gctgtccgat
     2461 gtgaccttgg gcatcaacaa gagcttcatc aaggtcaatc gcttcctggc cgaccaccag
     2521 gtgctgcccg agatcaaggc gcagctgcgc gcgcggagcg agctgcggcc gcgcgacgat
     2581 gccgaattgc tgccgacctt cctgcgactg ttcaaggagg gccgctcggc tgccgccgac
     2641 cagttgctgg cgctggggat cgcggttccc gcagcgtcgg cgtccacgtc gaccgcgctg
     2701 aaggatttgc cgaagcctgc gcctgcgcac gcggccccgg cttcgacgcc gggcggcgat
     2761 tcgtcgcaag ccgaatcggc gccgccggcg ccatccgccg cgcccgcgcc gccgacgatc
     2821 gataccggac cgggtgcggc gctgagcgtc gcgctcaatc cctacatcgc cgatctcgcc
     2881 cgggcgttgc agtcgtccga ggccgcaact gcgccggcga ccggtatgtc gggcctgccg
     2941 caagtcgatc cgttgatggc gatcggttcg ctgtccgccg ccgtcgctgt cctcgatcgc
     3001 tggcagcgac tggatccggg gtccgatcac cgcgctgccg aagctgcgtc cggcgatgcc
     3061 gcaagccgcg ccgccgcgat gaaccgcatc ccgttcatcc gggcggcgat cgacgacaag
     3121 gttacgagct cgaccgacaa gatcacgatg gatgtgatcg gtctgctgtt cgactatatc
     3181 ttccgcgatc cgtcgatacc cgaagacctg cgaagcctgt tcacccgcct gcaggttccg
     3241 atcctgaaga cggcgctgct cgatcgcagt tttttctccg accgcaagca cccggcccgg
     3301 cggctgctcg atcatctcgc aggtgcggcg atcggcgcca ccggcgacgc cggctaccgc
     3361 gccgcattcg agctgacggc cgccggcgtg atcgaagagg tctgccgcga cttccaggtc
     3421 gacgtcggcg tcttcgacct tgccgatcga aaggtgcagg agttcgtcga tgccgagttg
     3481 caaaaagggg cgggcgcgct cggcaccgag gtcgccgctg cgctcgcggc ggaagagggg
     3541 gaggccgatc gctcggcggt gcgcgcgctg atccgcgaca agctctcagg tctcgatgtg
     3601 ccgttcgacg tgcgctcgtt cagcgaaacc atctgggccg actacctgac caacgtgcgt
     3661 ctggcgcagg gcagcgaagg cgacgagtgg cgccgcggtt tgcgtacgct cgacgacctc
     3721 ctctggagcc tgacggcgaa ggagcgcagt gcgcagaaag ctcggctcgc caagctcgta
     3781 ccgggaatga tccgcaatct gcgcgccggc gccgccgcgg tcaacgtcgg tgacgagcgc
     3841 gtcaagccct tgctcgatgc gatgtatcaa ctgcacatgg ccgcgatcaa gcctgcatcg
     3901 gcggacgagg tgcatgcacc cgccgcggcg gcggcgggtg ctggcgccgg caaggccgaa
     3961 gccggtgccg ggaaggctga agccggcgcc ccggtcgagg cggttgcgcc cgcaccgaaa
     4021 atcgccaacg tccatgattt cgtcgccgaa atggtcgtcg gcacgtggct cgcgttcggg
     4081 cgaggcgaca aaacggtcaa cgcgcgcctg tcgtggatca gcccgatgcg cagcaaatat
     4141 ctgttcacca cgcgcgagcg ctcgcgcgca atcgtcgcga ccccggagga cctcgcatgg
     4201 cagctcggtg cgggcaaggc gacgctgatc gtcgagccag tccccttgtt cgaccgcgcg
     4261 gtcagcgcgg cgctcgatac gctggccgcg caaaagccgc gcggcgacac cgcggccgct
     4321 gcctgaa
//
LOCUS       DATANS010000193         2797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_724351, whole genome shotgun sequence.
ACCESSION   DATANS010000193 DATANS010000000
VERSION     DATANS010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724351"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VHT22_02790"
     CDS             <1..563
                     /locus_tag="VHT22_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3136508.1"
                     /translation="RDMLTTVENVVERMNKLMLQLRTGATPVENARPVDLEAVVRRVC
                     AAKATLDPPIELELSTGVTAAGHEDRLEHVIGHLVQNAIDATSKGGSVTVKLHDDGAF
                     ALIDVKDTGTGMSSEFIRERLFRPFETTKPAGMGIGVYESAQYVEGLGGRIQVDSEPG
                     RGTCVRVLLPRGEIRTPATAVAGGAA"
     gene            560..1912
                     /gene="prsR"
                     /locus_tag="VHT22_02795"
     CDS             560..1912
                     /gene="prsR"
                     /locus_tag="VHT22_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HEX3136509.1"
                     /translation="MTETRRPLLIVEDDPELQKQMRWAFDRYETVVASDRESAIAQMR
                     RCEPAVVTMDLGLPPHPDDPSEGLLLLEEINAFAPDTKVIVLTGQNDRVNALKTIALG
                     AHDFCTKPFEPEILAWTIDRAFRVHELQEENRRLQTASAAGGMSGFMTRDPEALRVCR
                     TIEKVAPASATVLIQGESGTGKELLARALHDLSPRRDQRFVAINCAAIPETLLETELF
                     GYEKGAYTGAAKRTFGKFEMASGGTLFLDEVGDLPMALQAKLLRFLQERVIERIGGRE
                     EIAIDVRIVCATHQSLKDHIVSGRFREDLYYRIAEIVVEIPPLRARKGDAALLAHSFV
                     HKFAAEQRRGAMTLLPDAIASIEAHPWPGNVRELENVIKRAVIMADALTIGAADVGLG
                     PADAEPAILNLRRVREEAEKDAVLRVLGRVNGNYSKAAELLGVSRPTLYDLMHRCGLK
                     "
     gene            1949..>2797
                     /locus_tag="VHT22_02800"
     CDS             1949..>2797
                     /locus_tag="VHT22_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3136510.1"
                     /translation="MKLRYRICATLLALAALGLVGCGKDNPTALVASAKSYLAKNEYE
                     AGIIQLKSALQIAPDNGEARILYGKALLDTGRPAAAETEIRKAIELKYPADETYPLLA
                     RAMVDQNAYAKVVAELADRKLGDPKAQAELQTQVASAYLALGEIAKANEAIQAALVAV
                     PGDARALTIQARLAALGNDLPQALKLIDSAIAAAPNNAQALVVKAGFENAQGRRGEAV
                     KTLEHAVEISGGSLGARYALASMLIEIGQLDNAAAQVDAMKKLSPLEYRTLYADALVS
                     YSRGDAA"
BASE COUNT          566 a          839 c          942 g          450 t
ORIGIN      
        1 agcgtgacat gctgacgacg gtagagaatg tcgtcgaacg catgaataaa ctgatgctac
       61 agttgcgtac cggggcaacg ccggtggaaa atgctcgtcc agtcgatctg gaagcggtcg
      121 tgcgacgtgt ctgtgccgcc aaagcgacgc tcgatccacc gatcgagctg gaactatcta
      181 ccggtgtcac ggccgcgggg cacgaggatc gtctcgagca cgtgatcggc cacctggtgc
      241 agaacgcgat cgacgcgacg agcaaaggcg gcagcgtcac ggtcaagctg cacgacgacg
      301 gcgctttcgc gctgatcgat gtaaaggaca cgggaacagg catgagttcg gagttcatcc
      361 gcgagcggtt gttcagacca ttcgagacca cgaaacccgc gggtatgggg atcggcgtct
      421 acgagagcgc gcagtacgtc gagggactcg gcgggcggat acaggtcgac agcgagcccg
      481 gtcggggtac gtgtgtgcgc gtgctgctgc cgcgcggtga aatcagaacg cctgcgacgg
      541 cggtggcagg gggagccgca tgaccgaaac acgccggccg ctgctgatcg tcgaagacga
      601 tccggagttg caaaaacaga tgcgctgggc attcgaccgg tatgaaacgg tcgtcgccag
      661 cgatcgggaa tcggcgatcg cgcagatgcg ccgctgcgag ccggccgtcg tcacgatgga
      721 tctggggctg ccgccgcatc cggacgatcc gagcgaaggg cttttgttgc tcgaggaaat
      781 aaatgcattt gcgcccgaca ccaaggtcat tgtgctcacc gggcagaacg accgggtcaa
      841 tgcgctgaag acgatcgcgc ttggggcgca cgatttctgc accaagccat tcgaacccga
      901 gattctggcg tggacgatag accgggcgtt tcgcgtgcac gagttgcagg aagagaatcg
      961 ccgcctgcaa acggcgagcg cggcaggcgg catgtcgggc ttcatgacgc gcgatccgga
     1021 ggcattgcgc gtatgccgca ccatcgagaa ggtcgcgccg gcgtcggcaa cggtgctgat
     1081 ccagggcgaa tcaggaaccg gcaaggaatt gctggcccgc gctctgcatg atttgtcgcc
     1141 gcgccgcgat caaaggttcg tggcgatcaa ttgcgcggcg attccggaga cgctgctgga
     1201 aaccgagctc ttcggctacg aaaagggcgc gtacaccggt gcggccaagc gcacgttcgg
     1261 caagttcgaa atggccagcg gcggcacgct gttcctcgac gaggtcggcg atcttccgat
     1321 ggcgctgcaa gccaagctgc tgcgctttct gcaggagcgc gtgatcgagc gcatcggcgg
     1381 ccgggaggaa atcgcgatcg acgtgcgcat cgtctgtgcg acgcatcaaa gcctcaagga
     1441 tcacatcgtc tcgggacgct tccgggagga tctgtattac cggatcgccg agatcgtcgt
     1501 cgagattcct ccgctccgcg cccgcaaggg agacgcggcg ctgctggcgc acagctttgt
     1561 gcacaagttt gccgccgagc agcggcgcgg agcgatgacc ttgctgcccg atgcaatcgc
     1621 ctcgatcgaa gcgcacccat ggccgggcaa tgttcgcgag ctagaaaatg taatcaagcg
     1681 ggccgtcatc atggccgacg ccctgacgat aggcgcggcc gacgtgggtc tggggcctgc
     1741 cgatgcggag ccggcgatcc tgaacctgcg tcgcgtgcgc gaggaggccg agaaagacgc
     1801 cgtcctgcgc gtgctgggac gagtcaacgg caattacagc aaggccgccg agctgctcgg
     1861 cgtgagccgt ccgacacttt acgatctgat gcatcgctgc ggcctcaagt agagcgcacc
     1921 ggcgatcgat tccgcgaaag aagcgacaat gaagctccgg taccgtatct gcgcaaccct
     1981 cttagcgctc gcagcccttg ggcttgtcgg ctgcggaaaa gacaatccga ccgcgctggt
     2041 cgcatcggcg aagtcgtatc tcgccaagaa cgaatatgaa gccggcatca tccagctgaa
     2101 gagcgcgctg cagatagcac ccgacaatgg cgaggcgcga atcctgtacg gcaaggcgct
     2161 gctcgacacc ggtcgtccgg ccgcggccga gaccgaaatc cgcaaggcga tcgagctcaa
     2221 atatccggcg gacgaaacct atccattgct cgcgcgtgca atggtcgacc agaacgccta
     2281 cgcaaaggtg gtggcggagc tcgccgatcg caagctcggc gatccgaagg cccaggccga
     2341 gctccagacc caagtagcca gcgcctacct tgcgctcggc gaaatcgcga aagcaaatga
     2401 agcgatccag gctgcgcttg tcgcggttcc cggagacgcg cgcgcgctta cgattcaggc
     2461 tcgcctcgcc gccctcggca acgacctgcc gcaggccttg aagctgatcg attcggcgat
     2521 cgcggcggct cccaacaacg cccaggcgct ggtggtgaag gctggattcg agaatgcgca
     2581 aggccggcgc ggcgaagcgg tcaagacgct cgagcacgcg gtcgagatca gcgggggctc
     2641 gctgggcgcg cgctacgcgc tcgcgtcgat gctgatcgaa atcgggcaac tggacaatgc
     2701 ggcggctcag gtcgacgcga tgaagaagct gtccccgctc gagtaccgaa cgctctatgc
     2761 cgatgcgctg gtctcgtatt cgcgcggcga tgcggcg
//
LOCUS       DATANS010000194         1660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_345661, whole genome shotgun sequence.
ACCESSION   DATANS010000194 DATANS010000000
VERSION     DATANS010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345661"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VHT22_02805"
     CDS             complement(<1..426)
                     /locus_tag="VHT22_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3136511.1"
                     /translation="MATTWCESGGTGSRAFLLLHGLGATAAVWGGVRRAIEQRALGRW
                     IAPDLGGHGGSAWPGQYTVGGHAAEVAELVHGTQELFVIGHSLGAYIALALASRWFGV
                     EVAGVLGIGPKITWSQADLQGSRELAGRPVRWYATAEEAW"
     gene            470..1366
                     /locus_tag="VHT22_02810"
     CDS             470..1366
                     /locus_tag="VHT22_02810"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014983728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3136512.1"
                     /translation="MTTVAFIGLGNMGLGMAQRLLAAGHDLRVYNRTGSKAAELERAG
                     AKLCGTPREACADAAAVFTMTADDVSSRAVWSGPDGVLAAQLAPRAFAIECSTLSYNW
                     VMELAAQARVRSLRYVDAPVTGLPDAAAAGELTLLVGADAADLEAARPLLSAISNRIL
                     HFGAVGSGTAYKLIVNLMGAVQIASLAEGMALAERIGLDLDAVANAISTGQAASPQVV
                     RNARRIVADDHDRNVVFTPVLRLKDVDYALQLAQGIGMRAPFGQVAAGQLQQLIELGH
                     SRDNESRIIEIARLQRAGAAAL"
     gene            1426..>1660
                     /locus_tag="VHT22_02815"
     CDS             1426..>1660
                     /locus_tag="VHT22_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136513.1"
                     /translation="MRRVGLASILIFIAARSLAADAVAEPWVLLTQRDLQSIHDTLAA
                     NHPGPVDPQSDRYRRWLEDGLQAAKTQAAAARSY"
BASE COUNT          303 a          579 c          512 g          266 t
ORIGIN      
        1 ccacgcctcc tccgccgtcg cataccaacg cacgggtcga ccggcgagct cgcgcgagcc
       61 ctgcagatcc gcctgcgacc acgtgatctt cggcccgatg ccgagtacgc cagcgacttc
      121 gacgccgaac cagcgactcg caagtgcgag cgcaatgtaa gcgccaagcg aatggccgat
      181 cacgaacagt tcctgcgtgc cgtggaccaa ctccgcaact tcggcagcat ggccgccgac
      241 cgtgtattga cctggccacg ccgagccgcc gtgaccgcca agatccggcg cgatccaccg
      301 gcccagcgca cgctgctcga tcgctcgccg cacgccgccc cacaccgccg ccgtcgcgcc
      361 gaggccatgc aggagcagga aggcccgcga gccggtccca ccgctttcgc accaggtcgt
      421 tgccatagga agatatattg gcagcatcgc gcgaagagag acacagcccg tgacgacagt
      481 cgccttcatc ggactcggaa acatgggcct cggcatggcg cagcgcctgc tcgcggccgg
      541 tcacgatctg cgcgtttaca accgcaccgg ctccaaggcg gcggagctcg aacgcgctgg
      601 cgcaaagctt tgcggcacgc ctcgtgaggc ctgcgccgac gccgcagcgg ttttcacgat
      661 gaccgcagac gacgtctctt cccgtgcagt ctggtcggga ccggatggcg ttctcgctgc
      721 acaactggcg ccgcgagcat tcgcgatcga gtgctcgaca ctctcgtaca actgggtgat
      781 ggaacttgca gcccaggcgc gagtacgcag tcttcgctat gtcgacgcgc cggtcaccgg
      841 cttgccggac gccgccgcgg ccggcgagct gaccctgctg gtcggcgccg acgccgcaga
      901 tctcgaagca gctcgcccgc tgctgagcgc aatctcgaac cgcatcctgc atttcggcgc
      961 agttggctcc ggcactgcct acaagctgat cgtcaatctg atgggcgcag tccagatcgc
     1021 gtcgctagcg gagggcatgg ccctcgccga acggatcggc ctcgacctgg acgccgtggc
     1081 gaatgcaatc agcaccggcc aggcagcaag tccgcaggtc gtgcgcaacg cgcgtcgcat
     1141 cgtcgctgac gatcacgatc gcaacgtcgt gttcacaccg gtcttgcgct tgaaggacgt
     1201 cgactatgcc ttgcaactgg cgcaaggcat cggcatgcga gctcccttcg gccaggtcgc
     1261 cgccgggcag ctgcagcagc tgatcgagct cggtcattcg cgtgacaacg agagcaggat
     1321 catcgaaatc gccagattgc agcgggccgg cgcagccgcg ctttaaacct ctggcccctg
     1381 attcgtcatc caagtcataa gcagtctggg gaatgggata gcaccatgcg cagagtcggt
     1441 ctggcttcga ttctgatctt catcgcggct cgctcgctgg cagccgatgc tgtggcggag
     1501 ccgtgggtgc tcctgacaca acgggatctg cagtcgatcc acgacacact ggctgccaat
     1561 caccccgggc ccgtcgatcc acagagcgat cgttatcggc gctggctcga ggacggatta
     1621 caggccgcaa agacccaggc cgcggcagcc cgcagctaca
//
LOCUS       DATANS010000195         1833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_472580, whole genome shotgun sequence.
ACCESSION   DATANS010000195 DATANS010000000
VERSION     DATANS010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472580"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VHT22_02820"
     CDS             complement(<1..959)
                     /locus_tag="VHT22_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX3136514.1"
                     /translation="MKAGEHATPAPISVWRGKPRPLGSSWDGAGVNFALYSENAEAVQ
                     LCLFDPKGRREIARIDIRERTNFVWHCYLPEARPGQLYGYRVHGPYAPERGDRFNAHK
                     LLLDPYARLVAGPLRWTDAHYGYRVGSRREDLSFDTRDSASAMPKCRVIDSSFTWGND
                     IRPQTPWEHTVIYELHVKGFTQLHPAIRPQLRGTYAGLSTAPVIAHLQRLGVTAVELM
                     PVHLFVDDQALVQRGLRNYWGYNSIGFFSPDTRYAAGDAVNEFKTMVKTLHAAGIEVL
                     LDVVYNHTAEGNHLGPTLSFRGIDNRVYYRLDPENPRHYVDYTG"
     gene            complement(956..>1833)
                     /locus_tag="VHT22_02825"
     CDS             complement(956..>1833)
                     /locus_tag="VHT22_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEX3136515.1"
                     /translation="TVSPAIVPAPGQSVARGAAGASAAGAAATTAGTPTPHGSFLTLV
                     AMVGQRTGELHVALAAASGDPAFDPEPVSTDDVAAWIAAVVAEVDQTFDQLSQRQGDL
                     PPPVRAAAGRLLAMRASLKDRIAALAPRVLTFTKTRFHGDFHLGQLLVVEHDFIVVDF
                     EGEPFRPMDQRRRKHSPLRDVAGMLRSFSYAAAIALDEAISDRPADRVRAAAHMARWE
                     RETAAAFLTAYARAVRGASSYPSDPVDVARLIDLFVLEKALYEVRYELGSRPDWVRIP
                     IAGLLERMQVAAAVS"
BASE COUNT          319 a          584 c          638 g          292 t
ORIGIN      
        1 ccggtgtaat cgacgtaatg gcgcggattt tcggggtcga gacgatagta gacacgattg
       61 tcgataccgc gaaacgacag cgtcgggcca agatgattgc cttcggccgt atggttgtaa
      121 acgacgtcga gcaggacttc gatcccggcg gcatgcagcg tcttgaccat cgtcttgaac
      181 tcgttgaccg catcgcccgc ggcatagcgg gtgtccggcg agaaaaagcc gatgctgttg
      241 tacccccagt agttgcgcag cccacgttgg accagcgcct ggtcgtcgac gaacaggtgg
      301 acgggcatca gctcgaccgc ggtgacgccg agccgctgca aatgagcgat caccggggcg
      361 gtcgacaggc cggcataggt tccgcgaagc tgcggccgga tcgcgggatg cagctgggtg
      421 aaacccttga cgtgcaattc gtagatgacc gtgtgctccc acggcgtttg cggtcgtatg
      481 tcgttgcccc aggtgaacga ggagtcgatc acccggcact tgggcatcgc cgacgcgctg
      541 tcccgggtgt cgaagctcag atcttcgcgt cggcttccga cgcgatagcc gtaatgcgcg
      601 tcggtccagc gcagcggccc cgccaccaaa cgggcgtacg ggtcgagcaa cagcttgtgc
      661 gcgttgaacc gatcgccgcg ctccggcgcg tacggaccgt gcacccggta cccgtagagc
      721 tggccggggc gggcttcggg aagatagcaa tgccagacga agttggtgcg ctcgcgtatg
      781 tcgatccgcg ctatctccct ccggcccttg gggtcgaaca ggcacagttg gacggcttcc
      841 gcgttttccg aatacagcgc gaagttgacg cccgcgccat cccaggatga gcccaacggc
      901 ctcggtttgc cgcgccagac gctgatcggc gcgggcgtcg cgtgctcgcc ggctttcacg
      961 agaccgctgc cgcgacttgc atgcgttcga gcaggccggc gatcggaatg cgaacccagt
     1021 ccggacgcga gcccagctcg tagcggacct cgtagagcgc cttttccagg acgaacaagt
     1081 cgatcagccg cgccacgtcg acgggatcgg atggatacga gcttgcgccg cggaccgcgc
     1141 gcgcgtaggc ggtcaaaaac gccgctgcgg tctcgcgctc ccagcgggcc atgtgcgcgg
     1201 cggcgcgaac acgatcggca ggccggtcgc tgattgcctc gtccagcgca atggcggcgg
     1261 catagctgaa cgagcgcagc atgccggcga cgtcgcgcag cggcgaatgc ttgcgccgac
     1321 gctggtccat cggacggaac ggctcgccct cgaaatcgac gacgatgaaa tcatgctcga
     1381 ccactagcag ctggcccaga tggaagtcgc cgtggaaacg ggttttggtg aacgtcagga
     1441 cgcgaggtgc cagcgcggcg atgcggtcct ttaagctcgc acgcatggcg agcaggcgtc
     1501 cggccgccgc acgtacgggc ggtggaagat cgccctggcg ctgcgatagc tgatcgaagg
     1561 tctgatcgac ctcggcgacg accgcggcaa tccatgcggc aacgtcgtcg gtcgacaccg
     1621 gttcgggatc gaacgcggga tcgccggacg cggcggcgag tgcgacatgc agctccccgg
     1681 tgcgctgccc gaccatcgcg accagcgtga gaaagctgcc gtgcggcgtc ggcgtgccag
     1741 cggtggtcgc cgcggcgccc gcagcagacg cgccggccgc gccgcgcgcg acgctttggc
     1801 cgggtgccgg cacgatggcc ggcgaaacgg tcg
//
LOCUS       DATANS010000196         2621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_94584, whole genome shotgun sequence.
ACCESSION   DATANS010000196 DATANS010000000
VERSION     DATANS010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94584"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VHT22_02830"
     CDS             complement(<1..846)
                     /locus_tag="VHT22_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEX3136516.1"
                     /translation="MSVRQYGPGIVVSGPVTAAYAEVLTPEALAFAARLQRAFGGRRT
                     ELLAHRADRQAQLDAGRLPDFLPETRAIRDANWTCAPVPADIEDRRVEITGPVDRKMI
                     INALNCGANVFMADFEDANTPTWDNNIQGQINLRDAVRRQIDYVSADGKAYRLDEKTA
                     TLFVRPRGWHLPEKHVLIDGAAISGAIFDFALYFFHNAKELIVRGTGPYFYLPKMESH
                     LEARLWNDIFVLAQDELGVPRATIKATVLIETILAAFEMDEILYELRDHSAGLNCGRW
                     DYIFSC"
     gene            complement(971..1687)
                     /locus_tag="VHT22_02835"
     CDS             complement(971..1687)
                     /locus_tag="VHT22_02835"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEX3136517.1"
                     /translation="MQASRTGSPPPAGEQATLPASVRVGAVELSRWPDGQGVAPLSAP
                     LMAITTFNDHADYHPALISAALAAENDPRFRGEMFRGACGTKVRNIPGWDASAAALIH
                     GRALMLAHHTLSRKPVYADDSWGSIYHTGDYCMPHSHLRSNASIVYMLDPGDDDPGEP
                     MAGKLCFADARIPVCCPHEPGRVTQHLIPTMTPGTMIIFASDYLHSVNPYRGRRPRIT
                     LSWNITVERLPGRPGEGWDK"
     gene            complement(1727..>2621)
                     /locus_tag="VHT22_02840"
     CDS             complement(1727..>2621)
                     /locus_tag="VHT22_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018504505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEX3136518.1"
                     /translation="TGNVCLVTLAMNLYSQGIDPGLDFSDIGVIIRTAEYCTDLPVHP
                     RHPYAGELVFTAFSGSHQDAIKKGLAAQTEAEAAGRALWEVPYLPIDPADVGRTYEAV
                     IRVNSQSGKGGIAYLLERDYGLALPRLLQIEFSQVIQQITDSTGKELSASEIHAAFER
                     EYLRASTPIAYVDHRAQHSAADGAIEHLTARLKIDGVEAVLSGDGNGPVDAFIHALRT
                     ATGLDVHVLNYHEHGVGSGEDATAVAYVQLRVGADRTCYGVGLDPNIVTATLRAVISA
                     TNRAVERGWASLPHARRVANA"
BASE COUNT          444 a          835 c          877 g          465 t
ORIGIN      
        1 gcagctgaag atgtaatccc agcggccgca attgagcccg gccgaatgat cgcgcagctc
       61 atacaggatc tcgtccatct cgaacgccgc gaggatcgtt tcgatcagca ccgtggcctt
      121 gatcgtcgcg cgcggcacgc caagctcgtc ctgcgccagc acgaagatgt cgttccacag
      181 ccgcgcttcg aggtgactct ccatcttggg cagatagaaa taaggtccgg tgccgcggac
      241 gatcagctcc ttcgcgttgt gaaagaaata cagcgcgaag tcgaatatcg cgcccgaaat
      301 cgcggcgccg tcgatcagga cgtgcttttc gggcaggtgc cagccacgcg gccgaacgaa
      361 caacgtggcg gtcttctcgt ccagccgata cgccttgccg tccgcgctca cgtagtctat
      421 ttgccgccgc acggcgtccc gaaggttgat ctggccctgg atgttgttgt cccaggtcgg
      481 cgtattggcg tcctcgaaat ccgccatgaa gacgtttgcg ccgcaattga gcgcgttgat
      541 gatcatcttg cggtcgaccg gacccgtgat ttcgacacgg cgatcctcga tgtccgcggg
      601 taccggcgcg cacgtccagt tcgcgtcgcg tatggcgcgc gtttcaggca ggaaatcggg
      661 cagccggccg gcgtcgagtt gcgcctggcg gtcggcacgg tgcgccagca gctcggtgcg
      721 gcggccgccg aaggctcgct gcaggcgagc ggcaaaggcc agggcctccg gcgtaaggac
      781 ttcagcgtag gccgcggtga ccgggccgga aaccactatg ccggggccgt actggcgaac
      841 gctcatcgac gatccctttt ttgcggcgcg ggccggccca acccgaggtc cggacggcag
      901 ccgaccggcc atgttgcagt gcggaaatca taccagaacc gcactttcag ggtgccccgt
      961 cggatcgccg ctatttatcc caaccctccc cgggtcggcc gggaagccgc tcgacggtga
     1021 tattccacga cagcgttatc cgcgggcgcc gcccgcggta gggattgacg ctgtgcaggt
     1081 agtcgctggc gaaaatgatc atcgtgcctg gggtcatggt cgggatgagg tgctgggtca
     1141 cgcggccagg ctcgtgcggg cagcagacgg ggatgcgcgc atcggcgaag cagagcttgc
     1201 cggccatcgg ctcgcccgga tcgtcgtcgc cgggatcgag catgtagaca atgctcgcat
     1261 tggagcgcaa atgactgtgc ggcatgcaat agtcgccggt gtggtagatg ctgccccaac
     1321 tgtcatccgc gtagaccggc ttacgcgaca gcgtgtggtg ggccagcatc aacgcgcggc
     1381 catggatcag cgcggcagcg gaggcatccc accctgggat attgcggacc ttggtgccgc
     1441 aggcgccacg aaacatttcg ccgcgaaatc gcggatcgtt ttcggccgcg agtgccgcac
     1501 tgatcagcgc cggatgatag tcggcgtgat cgttgaatgt cgtgatcgcc atcagcggcg
     1561 ccgacagcgg agcgaccccc tgcccgtccg gccagcgact gagctcgacg gcgccgacgc
     1621 gcaccgatgc gggcagtgtc gcctgctcgc cggcgggcgg cggcgatccg gtgcgcgacg
     1681 cctgcatgcg ttgggctaga cggcgaccgc ggcgcggccg ccgcggtcag gcattcgcga
     1741 cccgtcttgc gtgcggcaaa ctcgcccatc cgcgctcgac cgcgcgattg gtcgcgctga
     1801 tcactgcgcg cagtgtcgcg gtaacgatgt tcggatcgag accgacgcca tagcaggtcc
     1861 ggtcggcgcc gacccgcaac tgcacgtagg caacggccgt cgcatcctcg ccgctgccga
     1921 ctccgtgctc gtggtaattg agcacatgca cgtccagtcc ggtcgcggtg cgcagcgcat
     1981 ggatgaaggc atcgaccggt ccgttgccgt cgccggacag caccgcctcg acgccgtcga
     2041 tcttgagccg cgcggtcaga tgctcgatcg cgccatcggc ggcgctgtgc tgtgcccggt
     2101 gatcgacata cgcgatcggc gttgacgccc gcagatattc acgctcgaac gcggcgtgaa
     2161 tttcggatgc ggacagttcc ttgcccgtcg agtcggtgat ctgctgaatg acctggctga
     2221 attcgatctg cagcaggcgc ggcagcgcga gtccgtaatc gcgctcgagc aggtaggcga
     2281 tgccgccctt gcccgactgg ctgttgactc ggatgacggc ctcgtacgtg cggccgacat
     2341 cggcggggtc gatcggaaga tacggtactt cccacagcgc cctgccggcg gcctcggcct
     2401 cggtctgcgc ggccagacct ttcttgatcg cgtcctggtg cgagccggaa aacgcggtga
     2461 acaccagctc gcccgcatat ggatggcgcg gatgaaccgg cagatcggtg cagtactcgg
     2521 cggtacggat gattacgcca atgtcggaaa aatcgagacc cggatcgatt ccctgtgagt
     2581 aaaggttcat cgccagcgtt accaggcaga cgttgccggt g
//
LOCUS       DATANS010000197         1666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_9610, whole genome shotgun sequence.
ACCESSION   DATANS010000197 DATANS010000000
VERSION     DATANS010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9610"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..993
                     /locus_tag="VHT22_02845"
     CDS             67..993
                     /locus_tag="VHT22_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3136519.1"
                     /translation="MSPLATHGRGVGRWLLLAAIAAIAVGTLAWYAARPRATSVATTP
                     VVTVYPSQQFVVLDSTGYVVASRKATISSKVSGRLEWLGVAEGSRVKAGEVIARLDGR
                     DVKTKQDMLIRAPFDGVILSKSADVGDLVTPFSGTADSKRAVASIADMSKLEVEADVP
                     ASGLAKIRVGQPCEIVLDALPDARFLGTVSRIVPSVDRAKATVKIQFAAIDQRVLPEV
                     SARVLFLSQPITAEQQLPLTAVNPRALATRDGRTVLYVLREDRAVEVAVTPGRRLGEL
                     TAFTGDAKSGDKAVLDPRAELKAGALIEPAKK"
     gene            complement(1034..>1666)
                     /locus_tag="VHT22_02850"
     CDS             complement(1034..>1666)
                     /locus_tag="VHT22_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136520.1"
                     /translation="FIAEAAAAGLPRSSSSQTGLTNADVELPVAPWWPLAAALLVVGA
                     LARWLVPMRRRSLEPPAWVAPTARTEPNPSVSGASTSRTFGKTGHFAAMTEPGPEFSR
                     ASTAQAERTGTGARTTTRSQPGAVKGAATIEDHRFAEFDADLLEEQTIRAEWAKAVSD
                     GAIDIGSDSILKAIDEAERETQGAPAHPAQVALDRSLDDELLPNKPRGRA"
BASE COUNT          269 a          540 c          570 g          287 t
ORIGIN      
        1 cgggcatatg ctgcaccacg atcaaccggc gagcgtggcg gcggcaatcg agccctttct
       61 ctccgggtga gcccactggc tacgcacggt cgaggcgtgg gacgctggtt gttgctggcc
      121 gccatcgcgg cgatcgccgt cggcaccttg gcatggtatg cggcgcggcc ccgcgccacc
      181 agcgtggcga cgacgcctgt cgtcaccgtc tatccatcgc agcagttcgt ggtgctcgac
      241 tcgaccggct acgtcgtcgc gtcgcgcaag gccacgatat cgtcgaaggt ttccggccgc
      301 ctcgaatggc tgggcgtggc cgaaggttcg cgagtcaagg cgggcgaggt catcgcgcgg
      361 ctggacggcc gcgacgtcaa gacgaagcag gacatgctga tccgcgcgcc gttcgacggc
      421 gtcatccttt cgaagagcgc cgatgttggt gatctggtca ccccgttttc ggggacggct
      481 gattccaagc gcgcggtggc gagcatcgcc gacatgagca agctggaagt cgaggccgac
      541 gttcccgcgt cgggcctggc caagattcgt gtcggccagc cttgcgaaat cgttctggac
      601 gcgctgcccg acgcgcgctt tctcggcacc gtctcgcgta tcgtgcccag cgtcgaccgt
      661 gcgaaggcaa cggtgaagat ccaatttgcc gccatcgatc agcgcgtgct gccggaagtg
      721 agcgccaggg tcttgtttct ttcgcagccg atcaccgcgg agcagcagct gccgctaacc
      781 gcggtgaacc cgcgtgcgct ggcgacgcgc gacggcagga cggttctcta cgtgcttcgt
      841 gaggacaggg cggtggaagt ggcggtgacg ccgggccgca gactcggcga gttgactgca
      901 ttcaccggcg acgccaaaag cggcgacaag gcggtgctcg accctcgagc cgaactcaaa
      961 gccggcgcgc tgatcgagcc tgcaaagaaa tagcttaaac gattgcgagc tgaacgccgg
     1021 tcggagccag gcgctaagct cgaccgcgcg gcttgttcgg cagcagctcg tcgtcgagcg
     1081 aacggtcgag cgcgacctgt gccggatgcg ccggagcgcc ctgggtttcg cgttcggcct
     1141 catcgatcgc cttgaggatg gaatcgctgc cgatgtcgat tgcgccatcg ctgacggcct
     1201 tggcccattc ggcccggatc gtttgttctt ccagcaggtc agcgtcgaat tcggcgaagc
     1261 ggtgatcctc gatcgtcgca gcgcccttga ctgcgcccgg ctgcgacctg gtcgtggtcc
     1321 gcgctccagt tcccgtgcgc tcggcctgcg cggtactcgc gcgtgagaat tcggggccgg
     1381 gctcggtcat cgccgcgaag tgccctgtct tgccaaaagt cctagaagtg cttgcgccgg
     1441 aaaccgaagg gttgggttcg gtgcgcgcgg tcggcgcaac ccaagccggt ggttccagcg
     1501 aacggcgacg catcggcacc agccaccgcg caagcgcgcc gacgaccagc aatgcggccg
     1561 ctagcggcca ccagggtgcg accggcaact cgacatcagc gttcgtcaag ccggtctggc
     1621 tcgacgagga gcgcggcagt ccagccgccg ccgcttcagc aatgaa
//
LOCUS       DATANS010000198         2847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_681744, whole genome shotgun sequence.
ACCESSION   DATANS010000198 DATANS010000000
VERSION     DATANS010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681744"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VHT22_02855"
     CDS             <1..676
                     /locus_tag="VHT22_02855"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX3136521.1"
                     /translation="FPASRDEAAASMQMSLRWATRSKAAHDGSPNALFGIVQGGMYED
                     LRDESLAALAATGFDGYAIGGLSVGEPKEAMRRIEAHTAPRLPDDKPRYLMGVGTPED
                     IVEAVGHGIDMFDCVLPTRNARNGWLYTRLGDVKIRNARHRTDTNPLDQGCDCYTCRN
                     FSRAYLHHLQRANEILGARLNTIHNLHYYLTLAAELRQAIRDDALPAYAAAFRAGRAE
                     AEAADA"
     gene            845..1171
                     /gene="yajC"
                     /locus_tag="VHT22_02860"
     CDS             845..1171
                     /gene="yajC"
                     /locus_tag="VHT22_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX3136522.1"
                     /translation="MVLISPAYAQSTGGPFGGDLMAFLPMVAIFVVFYFLLIRPQQKR
                     AKETKAMLEALQKGDEVVTAGGVVGKIAKISEGYADVDIAPNVTITVQRSAISLMLPK
                     GTIKNL"
     gene            1250..>2847
                     /gene="secD"
                     /locus_tag="VHT22_02865"
     CDS             1250..>2847
                     /gene="secD"
                     /locus_tag="VHT22_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX3136523.1"
                     /translation="MNRYPAWKYILIAIALVVGIFYTLPNFFPEVPAVQVSTSKASVK
                     IDAGTLQTVEDALKAAGVPFNGALLDSNGVKVRLPDPDTQLKARDVLQQKFGDNYIVA
                     LNLLSTSPRWLASIGALPMYLGLDLRGGVHFLLQVDMKAALDKAADRYLTDIRSLLRE
                     KKVLYSGIAREGQNVAVRFRDGGERNRAYNEISASFPDLQLNNVDGGSDVKLVAGLKP
                     EAQKRIQEGAVQQNITILRNRVNELGVAEPIVQQQGADRVVVQLPGVQDTTRAKDIIG
                     RTASLEIRMVDDDPTAQQMALSGQVPLGDDKLTEPSGTPILVKRQVVLTGDRISDAQP
                     GFDGRNNEPAVHVNLDGAGARIFKEVTRENVGKRMAIVLVEKGKAEVITAPVIREEIG
                     GGRVQISGRMNTREANDIALLMRAGALAAPMEIVEERTVGPSLGKDNIEKGFDSVLYG
                     FVVLAVFNCSYYMLMGIISTISLAVNLLLLIAILSMLQATLTLPGIAAVALTLGMAID
                     ANVLINERIREELRWGDTPHAAIQA"
BASE COUNT          546 a          928 c          924 g          449 t
ORIGIN      
        1 tttcccggcg agccgggacg aggcggcggc atcgatgcag atgtccttgc gctgggccac
       61 gcggtcgaag gccgcccacg acggcagtcc caatgcgctg ttcggcatcg tgcagggcgg
      121 catgtacgaa gaccttcgcg acgaatcgct ggccgcgctg gccgcgaccg gcttcgacgg
      181 ttatgcgatc ggcggcctgt cggtcggcga gccgaaggag gcgatgcgcc gcatcgaagc
      241 ccacaccgcg ccgcggctgc cggacgacaa gccgcgctac ctgatgggcg tcgggacgcc
      301 ggaagacatc gtcgaggccg tcggccacgg tatcgacatg ttcgactgcg tgctgccgac
      361 gcgcaatgcg cgcaacggct ggctgtacac gcgtctcggc gacgtcaaga tacgaaacgc
      421 ccgtcaccgc accgatacga acccgctcga tcagggatgc gactgctaca cgtgccgcaa
      481 tttcagccga gcctacctgc atcatttgca gcgagccaac gaaatcctcg gcgcgcgcct
      541 caatacgatc cacaacctgc attactatct gaccctcgcc gcagaactgc ggcaggcgat
      601 ccgcgacgac gcgctgccgg cttacgcggc cgcctttcgg gcaggccgcg cggaagccga
      661 ggcggcggac gcctgagtcc ctccgcgcca ggaatccccg agcaccatcc aagcgcctgt
      721 cggggtccgg acagccgtct cgagcatggt agaattgcgg gttaaatcag cagccctaat
      781 gcgagtccgc cggctggccg caacaaacac ctagccaggc gaattccgat gcgaggagag
      841 agcaatcgtg ttgatcagcc ccgcctatgc ccagtcgacc ggaggtccgt tcggcggcga
      901 cctgatggcg ttcctgccga tggtcgccat cttcgtcgtt ttctacttcc tgctgatccg
      961 gccgcagcaa aagcgcgcca aggaaaccaa ggcgatgctc gaagcgctgc aaaagggcga
     1021 cgaggtggtc accgccggcg gcgtcgtcgg caagatagcg aagatctccg aagggtatgc
     1081 cgacgtcgat atcgcgccga atgtgacgat taccgtgcaa cgcagcgcga tatcgctgat
     1141 gttgcccaag ggcacgatca agaacctgta gaggcgcgga ctggtcgccg cccgcgagga
     1201 gcgcgagcgg cgcctcacgc gatacgcgcc gctcatcgcc ggacctccaa tgaatcgcta
     1261 tcccgcctgg aaatacatcc tgatcgccat cgccctggtc gtcgggatct tctacacgct
     1321 accgaacttc tttcccgagg ttcccgccgt tcaggtctcg accagcaagg ccagcgtcaa
     1381 gatcgatgcc ggcacgctgc agacggtcga agacgcgctg aaggcggccg gtgtcccgtt
     1441 caatggtgcg ctgctggaca gcaacggcgt caaggtccgc ctgcccgacc ccgacacgca
     1501 actgaaggcg agggacgtac tgcagcagaa attcggcgac aactacatcg ttgcgctgaa
     1561 cctgctgtcg acctctccgc gctggctggc gtcgatcggc gcgctgccga tgtatctcgg
     1621 tctcgacctg cgcggcggcg tccatttcct gctgcaggtc gacatgaagg cggcgctcga
     1681 caaggcggct gatcgctatc tgaccgacat ccgatcgctt ctgcgcgaga agaaggtgct
     1741 ttattcgggc atcgcacgcg agggccagaa cgtcgccgtg cgcttccgcg acggcgggga
     1801 gcgcaacagg gcctacaacg agatttccgc ctcttttccc gacttgcagc tcaacaacgt
     1861 cgacggcggc agcgacgtca agctggtggc ggggctgaag cccgaggcgc agaagcgcat
     1921 ccaggaaggc gcagtccagc agaacatcac gatcctgcgc aaccgggtca atgagctcgg
     1981 cgtcgccgag cctatcgtcc agcagcaggg cgccgaccgg gtcgtcgtgc agctgccggg
     2041 cgtgcaggac acgacgcgtg ccaaggacat catcggccgc accgcgtcgc tggaaattcg
     2101 catggtcgac gacgacccga ccgcgcagca gatggcgctc tcgggccagg ttccgctggg
     2161 cgacgacaag ctcaccgagc ccagcggcac gccgatcctc gtcaagcggc aggtcgtgct
     2221 gaccggcgat cgcatcagcg acgcacagcc gggcttcgat ggacgaaaca acgagccggc
     2281 ggtgcacgtg aatctcgatg gcgccggcgc gcgcatcttc aaagaggtca cgcgcgagaa
     2341 cgtcggcaag cggatggcga tcgtgctggt cgaaaagggg aaggccgaag tcatcaccgc
     2401 gccggtgatc cgcgaggaaa tcggcggcgg gcgcgttcag atcagcggcc ggatgaacac
     2461 gcgcgaagcc aacgacatcg cactgctgat gcgcgcgggg gcgctggcgg cgccgatgga
     2521 gatcgtcgag gaacgcaccg tcggtccgtc gctcggcaag gacaacatcg aaaagggctt
     2581 cgattcggtg ctgtacggct tcgtcgtcct cgcggttttc aactgctctt actacatgct
     2641 gatggggatc atatcgacga tctcgctggc agtgaacctg ctgctgctga tcgcgatcct
     2701 gtcgatgctc caggcgacgc tgaccttgcc gggcatcgcc gcggtggcgc tgacgctcgg
     2761 catggcgatc gacgccaatg tcctgatcaa cgagcgcata cgcgaagagc tgcgatgggg
     2821 cgacacgccg cacgcggcga tccaggc
//
LOCUS       DATANS010000199         1866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_136339, whole genome shotgun sequence.
ACCESSION   DATANS010000199 DATANS010000000
VERSION     DATANS010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136339"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="VHT22_02870"
     CDS             <1..1001
                     /locus_tag="VHT22_02870"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX3136524.1"
                     /translation="VAGPRPDAILSAKAFSNALVVLHAIGGSTNALIHLTAIAARRGI
                     EVDLDAFDALGRRVPVLVDLKPSGLHYMEHLHEAGGLSAVLRELRPLLHLDAPTVSGK
                     TLGEVIAAAEIIPAQQVVRRVDNPIHAGGAIAVLRGNLAPGGAVIKHASATEALLSHR
                     GRAVVFDSLDDLAARIDAADLDVVADDVLVLRNAGPRGAPGMPEAGYIPIPLKLARQG
                     VKDMVRISDARMSGTAFGTIVLHVTPEAAAGGPLALVESGDMIELDVGQRRLELCVDA
                     NELARRRTRLGAPEAADRTTGYRRLYVEHVLQADAGCDFDFMRADTMTGTAPIQAT"
     gene            complement(1032..>1866)
                     /locus_tag="VHT22_02875"
     CDS             complement(1032..>1866)
                     /locus_tag="VHT22_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136525.1"
                     /translation="PALHQELAALIAESLGDPSHYAASVDPTLLPAAAAALGLRVPAH
                     AIVSDVAGAQSFGAAHGYPVLLKSRRSRDGVTVAKHSELAGAIAALSPTPTTGQADPR
                     DALLVQAEIRGQPRSYQSVAWKGELLAGFAGERIHDEAGRGRQTGVNRYYRSDALREI
                     AASLARGFGISGLFSLEGVVAENGDFRLLDINRRLLGGAHRGSEFGVDHCAALHAAMH
                     GLPYAGRTDLDEGEEHICVSFPQEWLRDPESKWLRDYPVDVPWDEPELFEAMLDLHPA
                     R"
BASE COUNT          298 a          629 c          633 g          306 t
ORIGIN      
        1 ccgtggccgg tccgcgcccc gatgcgatcc tgtcggccaa ggcgttcagc aatgcactgg
       61 tcgtgctgca tgcgatcggt ggctcgacca atgcgctgat ccatctcacc gccatcgctg
      121 cgcgacgggg catcgaagtc gatctcgacg ccttcgatgc gctgggccgc cgcgttccgg
      181 tcctcgtcga cctgaagccg tccggcctgc attacatgga acacctgcac gaggccggcg
      241 gcttgagcgc ggtgctgcgc gagcttcgcc cattgctgca tctggacgcg ccgacggtat
      301 cgggcaagac actcggcgaa gtaatcgccg ccgccgaaat cattccggcg cagcaagtcg
      361 tgcgtcgcgt cgacaatccg atccatgcgg gcggagcgat cgccgtgctc agaggtaacc
      421 ttgcgccggg tggcgcagtg atcaagcatg cgtcggcgac cgaggcactg ctttcccatc
      481 gcggccgcgc cgtggtgttc gactcgctcg acgatctcgc ggcgcggatc gacgccgcgg
      541 acctcgatgt cgtggccgat gatgtgctgg tgctgcgcaa tgcagggcca cgcggtgcgc
      601 ccggcatgcc ggaggccggc tacatcccga ttccgctgaa gctcgcccgg cagggtgtca
      661 aggacatggt gcgcatctcc gatgcccgga tgagcggcac tgccttcggc accatcgtgt
      721 tgcacgtcac gccggaagcc gcggccggcg gaccactcgc gctggtcgag agcggtgaca
      781 tgatcgagct cgatgtcggg cagcgccggc tcgagctatg cgtcgatgcc aatgagctgg
      841 cgcggcgccg gacgcggctc ggcgcgcccg aggccgccga tcgcacgacc ggttaccgcc
      901 gcctttatgt cgagcatgtg ctgcaggccg acgccggttg tgatttcgac ttcatgcgcg
      961 ccgacacgat gaccgggaca gcgccgatcc aggcgacgtg atcgcggatt gcgccgccgg
     1021 cgggcgcgat cctaacgcgc cggatggaga tcgagcatcg cctcgaatag ctccggctcg
     1081 tcccatggca cgtcgaccgg gtagtcgcgc agccacttgc tttccggatc gcgcagccat
     1141 tcctgcggaa acgaaacaca gatgtgctct tccccctcgt cgagatcggt gcgtccggcg
     1201 taaggcagac cgtgcatcgc cgcatgaagc gccgcgcaat gatcgacgcc gaactcgctg
     1261 ccgcgatggg cgccgccgag gaggcggcga ttgatgtcga gcaggcggaa gtctccattc
     1321 tcagcgacga ctccctccag cgaaaagagg ccgctgatgc cgaatccgcg ggcaaggctg
     1381 gccgcgattt ctcgcaacgc gtcgcttcga taataccggt tgacccccgt ctgacgtccg
     1441 cggccggcct cgtcgtggat cctctcgccg gcgaatccgg ccagaagctc acctttccac
     1501 gccaccgact gatacgaccg cggctggccg cgaatctctg cctggaccag cagtgcatcg
     1561 cgcggatccg cttgaccggt tgtcggggtg ggcgagagcg ccgcaattgc acctgccaac
     1621 tcgctgtgct tggcgacggt cacaccatcg cgtgaacggc ggctcttgag gaggaccgga
     1681 tagccgtgcg cggcgccgaa cgactgggcg ccggcaacgt cgctgacaat tgcgtgcgcc
     1741 ggtacgcgaa ggccaagcgc ggcggcggcg gccggaagca aggtcgggtc gacgctggcc
     1801 gcgtagtgcg acggatcgcc gagcgactcg gcgattagcg ccgcgagctc ctggtgcagc
     1861 gccggc
//
LOCUS       DATANS010000200         2478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_136487, whole genome shotgun sequence.
ACCESSION   DATANS010000200 DATANS010000000
VERSION     DATANS010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136487"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VHT22_02880"
     CDS             complement(<1..798)
                     /locus_tag="VHT22_02880"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEX3136526.1"
                     /translation="MSPPSTLAEWLAYLETLHPKTIALGLERVESVASRLNVKPVCPV
                     ITVAGTNGKGSTCALLEQMLLADGYRVAMYTSPHLLRYNERVRIDGVEASDAALRDAF
                     AAVEAVRAGTPLTYFEFGTLGALWLFARQKLDALILEVGLGGRLDAVNVVDPDVAVVT
                     TVAIDHVDYLGATREDIGHEKAGIFRPAKPAICADPNPPSSLIAHAAAIGATLVRIGR
                     DYGYTAQSAQWDYWGPGGARHGLPYPALRGNYQLANASTALCAADALR"
     gene            complement(836..1705)
                     /gene="accD"
                     /locus_tag="VHT22_02885"
     CDS             complement(836..1705)
                     /gene="accD"
                     /locus_tag="VHT22_02885"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX3136527.1"
                     /translation="MSWLQKLLPPRIKASPGTGRKSMPEGLWVKCQSCEAVLYRTDLE
                     KNLNVCPKCNHHARLTARERVDQLLDAEGRYEIGSEVLPIDSLKFKDTRKYPERLSAA
                     LEETGETDALVVMQGSIKSTPLVLAAFEFEFMGGSMGSVVGERFVRGVRVAYDNRVPF
                     VCITSSGGARMQEGVSSLFQMAKTTAVLQELSKARLPFVSILTDPTMGGVSASFAMIG
                     DVVIAEPGALIGFAGPRVIEQTVREKLPEGFQRAEFLLEKGAIDMIVDRRALKDELPR
                     LLTLLQRQPLPTG"
     gene            complement(1725..>2478)
                     /gene="trpA"
                     /locus_tag="VHT22_02890"
     CDS             complement(1725..>2478)
                     /gene="trpA"
                     /locus_tag="VHT22_02890"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX3136528.1"
                     /translation="YVTVGDPTLEHTVPILRALVKAGADVIELGVPFSDPMADGPVVQ
                     RASERALAQGVGLADVLAMVAAFRQRDATTPIVLMGYANPIEAMGEGEFVERATAAGV
                     DGVIVVDYPPEEARDFAALAAARGLAPIFLLSPTTPIARMSLVADMARGYVYYVSLKG
                     VTGAGHLDTAEVAARLAEIRRHVSLPVGVGFGIRDAASARAIAAHADAVVIGSRIIEE
                     IECGPADEAPARAGAWLATIRAAVDQVAKRAA"
BASE COUNT          412 a          868 c          799 g          399 t
ORIGIN      
        1 gcgcaaggca tcggccgcgc acagtgcggt cgacgcattg gccagctgat agttgccccg
       61 cagcgccgga taaggcaggc catgccgcgc tccgcccggt ccccagtaat cccattgcgc
      121 gctttgtgcg gtgtagccgt agtcgcggcc gatgcgcacc agcgtcgcgc ctatcgccgc
      181 ggcgtgcgcg ataagcgacg acggcgggtt ggggtcggcg cagatcgccg gcttggcagg
      241 gcggaaaatg ccggcctttt catgaccgat gtcctcccgc gtggcgccga gataatcgac
      301 atggtcgatg gcgaccgtcg tgaccaccgc cacatcggga tcgaccacat tgaccgcgtc
      361 gagacgcccg ccgaggccga cttcgaggat cagcgcgtcg agcttttgcc gggcgaacag
      421 ccacagcgcg ccaagcgtac cgaattcgaa ataggtgagc ggcgtcccgg cgcgcacagc
      481 ctcgaccgcg gcgaaagcat cgcgcagcgc ggcatcgctc gcctcgacgc catcgatgcg
      541 cacgcgctcg ttgtagcgca acaggtgcgg cgacgtgtac atcgcgacgc ggtaaccgtc
      601 ggcgagcagc atctgctcca gcagcgcgca cgtcgagccc ttgccgttgg tcccggcgac
      661 ggtgatcacc ggacagaccg gcttcacatt cagccgcgag gcgacgctct ccacgcgctc
      721 gaggcccagc gcaatcgtct tcgggtgcag tgtttcgaga tacgcgagcc actcggcaag
      781 ggtggagggc gggctcacgg gacggcggcg cgggatgcgc ccggggaatc gcgcgtcagc
      841 cggtcggcag cggctgccgc tgcagcaagg tcagcagtct cggcagttcg tccttcagcg
      901 cgcgtcggtc gacgatcatg tcgatcgcac ctttttccag caggaactcg gcgcgctgga
      961 atccctccgg cagcttttcg cggaccgtct gctcgatcac tcgcgggcct gcgaagccga
     1021 tcagcgcgcc cggctcggcg atcaccacat cgccgatcat cgcgaagctc gccgacacgc
     1081 cgcccatcgt cggatcggtc agtatcgaaa cgaacggcag ccgcgccttc gacaactcct
     1141 gcaagaccgc tgtcgtcttg gccatctgga acagcgagct gacgccttcc tgcatgcgcg
     1201 ctccgccgct cgacgtgatg cagacgaacg gtacgcgatt gtcgtacgcc acgcgcacgc
     1261 cacggacaaa acgctcgccg acgaccgagc ccatcgaccc acccatgaac tcgaactcga
     1321 acgctgcgag caccagcggc gtcgacttga tgctgccttg catcacgacc agcgcgtcgg
     1381 tctcgccggt ttcctcgagc gccgccgaca ggcgctccgg atacttgcgc gtgtccttga
     1441 acttcaggct gtcaatcggg agcacttccg acccgatctc gtagcgccct tccgcatcga
     1501 gtagctggtc gacgcgctcg cgcgcggtca ggcgcgcatg atgattgcac ttggggcaga
     1561 cgttgagatt cttctcgaga tcggttcgat acagcacggc ttcgcaggac tggcatttga
     1621 cccacagccc ttcgggcatc gacttcctgc ccgttcccgg cgatgccttg atgcgcggcg
     1681 gcaagagctt ctgcaaccaa ctcatacggt ctccccgggt cccgtcaggc ggctcgtttg
     1741 gcgacttggt cgacagccgc gcgtattgta gccaaccagg cgcccgcgcg cgccggcgct
     1801 tcgtccgcgg gcccgcattc gatctcctcg atgatacggc tgccgatcac cacggcatcg
     1861 gcatgcgcgg cgatcgcccg cgcgctggcc gcatcgcgga tgccgaagcc gacgccgacc
     1921 ggaaggctga cgtgccttcg tatttccgcg agtctcgcgg caacctcggc ggtgtcgaga
     1981 tgaccggcgc cggtgacacc cttcagcgag acatagtaga cgtagccgcg cgccatgtcg
     2041 gcgacgaggg acatgcgcgc gatcggcgtg gtcggcgaca acaggaagat cggcgctagg
     2101 ccgcgcgcgg cggcgagcgc cgcgaaatcc cgcgcttcct cgggtggata atcgacgacg
     2161 atgacgccgt cgacgccggc cgcggtcgca cgctcgacga attctccttc gcccatcgcc
     2221 tcgatcggat tggcgtagcc catcagcacg atcggcgtcg tcgcgtcgcg ctggcggaac
     2281 gcggcgacca tcgccagcac gtcggcgagt cccacgccct gcgccagcgc gcgctcggaa
     2341 gcgcgctgga ccaccggacc gtcggccatc ggatccgaaa acggtacgcc gagctcgatc
     2401 acgtcggcgc cggcctttac gagcgcccgc aggatcggca ccgtgtgctc gagcgttgga
     2461 tcgccgaccg tcacgtac
//
LOCUS       DATANS010000201         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_389011, whole genome shotgun sequence.
ACCESSION   DATANS010000201 DATANS010000000
VERSION     DATANS010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389011"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VHT22_02895"
     CDS             complement(<1..524)
                     /locus_tag="VHT22_02895"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX3136529.1"
                     /translation="MKSCWIVVKDHKAALEWRDLPVPQPKANEIVVRVRATALNRGEL
                     VVGGAVHGGPEKIGGTEAAGEVHAIGAGVTGVKAGDRVFGRVRGGFAEYVAMDAAQII
                     SLPAKLTFEQGAAVPISYITAYEMLYPPYGKLKAGEWLLITGASAGAGVASIQIAKML
                     GAKTLGTSGSAAKL"
     gene            612..1364
                     /locus_tag="VHT22_02900"
     CDS             612..1364
                     /locus_tag="VHT22_02900"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX3136530.1"
                     /translation="MDKTAVKLLRLYEHLCRADAPLGVSALAARLRLTKSNAHRILRT
                     LTGLGYAQQTADGQYAPTLKAWELGTLVINRLDFVQIAKPYLNELNVTTGESAYLATL
                     NGSSVVYLDVLESRYPIRIRSVIGGSAPAHCSASGKLLLAYNPLIAEQYLAKPLEKYT
                     ARTLTKLPDLERKFEAIRRDGYSINDGEWVEGVCGVSGPIHDGHRQGIAAIGVTALKE
                     RVNRAKLKQLTKLVTDVAGRVSAKLGYRAPVR"
     gene            complement(1378..>2010)
                     /locus_tag="VHT22_02905"
     CDS             complement(1378..>2010)
                     /locus_tag="VHT22_02905"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136531.1"
                     /translation="GDAAALFADLGRTYPHVATAIKKYPSCACNHTAIEAALHLVHEH
                     DVTPADAGAIEVTISPYVHRLVGAPFEPAADPQVTAQFSVQYSVACAILRRRLSVADI
                     QDAAVLEPAIGELARSVKVIIDDKRGNSRGATVRIRSRRHGLLEKHVEHIPGSPESPL
                     TPADLQSKFRECAIEGVSPLGTERGELLCARINGVEDVADMAGFFNGIAE"
BASE COUNT          347 a          687 c          620 g          356 t
ORIGIN      
        1 tccagcttcg ccgcggagcc ggaagtaccc agcgtttttg cgccgagcat tttcgcgatt
       61 tgtatgcttg cgacgcccgc gccggccgag gcgccggtga tcagcagcca ctcgcccgct
      121 ttcagtttcc cgtacggcgg atacagcatt tcatacgcgg tgatatagga aatcgggacc
      181 gccgcgccct gctcgaacgt gagcttggcc ggcagcgaaa tgatttgcgc cgcgtccatc
      241 gcgacgtatt ccgcaaagcc gccgcgcacg cggccgaaca cgcggtcgcc cgctttcacg
      301 ccggtcacgc ccgcgccgat cgcgtgcact tcacccgcgg cttcggtgcc gccgattttt
      361 tcagggccgc catgcaccgc gccgccgacg acgagctcgc cgcggttcag cgccgtcgcc
      421 cgcactcgta caacgatttc attcgccttc ggctgcggca ccgggaggtc gcgccattcc
      481 agcgcggcct tgtggtcttt gacgacgatc cagcaggatt tcatgtttgc ctcgcgttgt
      541 gaactcccgc gcagtttagc attggccgaa ttgccgctcc ctgccggctc gtgtaaagtc
      601 ggcgcccacc catggataag acagccgtaa aactcctgcg cttgtacgaa cacctgtgcc
      661 gcgccgacgc gccgctcggc gtttccgcgc tcgccgccag gctgcgcctg accaagagca
      721 acgcgcaccg catcctgcgc acgcttacgg gactcggtta tgcgcagcaa accgccgacg
      781 gccagtacgc gccgacgctt aaagcatggg agctgggcac gctcgtcatt aaccggctcg
      841 actttgtgca gatcgccaag ccttatctga acgagctcaa tgtcaccacc ggcgaatcgg
      901 cctacctcgc gacgctcaac ggcagctcgg tcgtctatct cgacgtgctc gaaagccgtt
      961 acccgatccg catccggtcg gtgatcggcg gcagcgcgcc cgcgcattgc agcgccagcg
     1021 gcaagctcct gctcgcctac aatccgctga tcgccgagca gtatctcgcg aaaccgctcg
     1081 aaaaatacac cgcgcgcacg ctcacgaaac tccccgacct cgagcgcaag ttcgaagcga
     1141 tacgccgcga tggctactcg atcaacgacg gggaatgggt ggaaggcgta tgcggcgtgt
     1201 cggggcccat ccacgacggc caccgccaag gcatcgcggc gatcggcgtc acggcattga
     1261 aggaacgcgt gaaccgcgcg aagctgaaac agctgaccaa gctcgtgacc gacgttgcgg
     1321 ggcgcgtatc ggccaagctc ggctatcgcg cgccggtgcg ctgattcggt ttttcgctta
     1381 ttccgcgata ccgttaaaaa agccggccat atcggctacg tcttcgacgc cgttgatgcg
     1441 ggcgcacaac aattcgccgc gctccgtacc cagcggcgag acaccctcga ttgcgcattc
     1501 gcggaatttg ctctgcaggt cggccggagt caacggcgac tcggggctgc ccggaatgtg
     1561 ctcgacatgc ttttcgagca ggccgtgccg gcgtgatctg atgcgcacgg tcgcgccgcg
     1621 gctgttgccg cgcttgtcgt cgatgatgac cttcacgctg cgcgcaagct cgccgatcgc
     1681 cggctccagc accgcggcgt cctgaatatc ggcgacgctc agccggcggc gcaggatagc
     1741 gcacgcgacc gaatactgga cgctgaactg cgcggtgacc tgcggatcgg cggcgggctc
     1801 gaacggcgcg ccgacgaggc gatgcacgta gggcgagatc gtgacctcga ttgcccccgc
     1861 atcggccggc gtcacgtcat gctcgtgcac gagatgcaga gccgcctcga tcgcggtgtg
     1921 attgcacgcg cagctcgggt atttcttgat cgcggtcgcc acgtgcgggt aagtgcggcc
     1981 gaggtcggca aacagcgcag cggcatcgcc
//
LOCUS       DATANS010000202         2186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_346440, whole genome shotgun sequence.
ACCESSION   DATANS010000202 DATANS010000000
VERSION     DATANS010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346440"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1658)
                     /locus_tag="VHT22_02910"
     CDS             complement(<1..1658)
                     /locus_tag="VHT22_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136532.1"
                     /translation="MRSTTSFISPRGGAAAAVLLLSLCIGGSGHADSLAVVDPLPPGP
                     YAVGCSNVAQDFSRVQPGESAQNYWEGYPDGSRERYVTQLLADPADALVVDVTVPDDR
                     DLYTNRAAQHVPYALLVCYPTSADNPYPDYALPSGQSVPHMQQGAQPPIFADANARWP
                     VLLFSHGLVGSPISSDYIAALTVLASYGYVVVAPFHGDPRFTDVKIEDLSDLLYALLH
                     FGSFVEMQALRPLSLSRSLDYLFAHPQYRDHLDVNRVGGFGASQGGESLLLMTGAKLT
                     ITVGMSSKQVLADPRLKAIVGYVPYLGQPFFPAFGRDQSGLDGISTPFLAISGGADTT
                     APLETTLEGVDRLTGSRDVVVLAGVDHGFDYASTNDIFTWSMTFLAAHVNDDRAARVR
                     IARMTTVRGGGNDFLVRDYTAPAPLLPGELDTIEYFGAGLGDYFLTAAPEEAAVLDAG
                     LLPGWARTGFAFKSWSIDAGHGVPACRFFGKPGPGPYAHFYTIDANECAFIAASPAWI
                     FEGTPFSEDPPAAGDCSADRIPVMRVYNNFMNGQIVHRFLTSKS"
BASE COUNT          417 a          717 c          710 g          342 t
ORIGIN      
        1 tcgctcttgc tcgtcaggaa gcggtgcacg atctgcccgt tcatgaaatt gttgtatacg
       61 cgcatcaccg ggatgcgatc ggcagagcaa tcgccggccg ccggcggatc ttcggaaaac
      121 ggcgtgcctt cgaatatcca cgccggactc gccgcgatga acgcgcattc attggcgtcg
      181 atcgtgtaga aatgcgcgta cggccccggt cccggcttgc cgaagaagcg gcatgccgga
      241 acgccgtgac cggcatcgat cgaccacgac ttgaaagcga acccggtgcg agcccagccc
      301 gggagcagac cggcgtcgag caccgcggcc tcctccggcg ccgccgtcag gaaatagtcg
      361 ccgagcccgg cgccgaagta ttcgatcgtg tcgagctcgc ccggcagaag cggcgccggc
      421 gcagtgtagt cgcggaccag gaagtcgttg ccaccgccac gcaccgtcgt catgcgggca
      481 atccgcaccc gcgccgcgcg atcgtcgttg acgtgcgcgg cgaggaaggt catcgaccag
      541 gtgaagatgt cgttggtgct cgcataatcg aacccgtgat cgacgccggc cagcacgacc
      601 acatcgcgcg acccggtgag ccgatcgacg ccttcgagcg tcgtctccag cggagccgtc
      661 gtatcagcgc cgccggaaat ggcgaggaac ggcgtgctga tgccgtcgag cccgctctga
      721 tcgcggccaa acgcggggaa gaacggctgc ccgagatagg gcacgtagcc gacgatcgcc
      781 ttcaggcgag gatcggcgag cacctgtttg gacgacatgc cgacggtgat cgtcagcttg
      841 gcgccggtca tcagcagcag cgactcgccc ccctggctcg cgccaaagcc gccgactcgg
      901 ttgacgtcga ggtgatcgcg atactgcggg tgggcgaaca gatagtcgag cgagcgcgac
      961 agcgacagag gccgcagcgc ctgcatttcg acaaaggacc cgaagtgcag cagcgcatag
     1021 agcaaatcgg agagatcctc gatcttgacg tcggtgaagc gcggatcgcc gtgaaatggc
     1081 gcgacgacaa cgtagccgta actcgccaga accgtcagcg ccgcgatgta gtcgctcgaa
     1141 atcggactac cgacgagccc gtgcgaaaag agcagcaccg gccaccgcgc attggcgtcg
     1201 gcgaatatcg gcggctgcgc accctgctgc atgtgcggca ccgattgccc cgagggcagc
     1261 gcgtagtcgg gatatgggtt gtcggcgctg gtcggataac agaccagcag cgcataggga
     1321 acatgctgcg ctgcgcgatt ggtgtagagg tcccgatcgt cgggcaccgt gacatcgacc
     1381 accagcgcat cggcggggtc ggccagcaac tgcgtgacat agcgctcgcg gctgccgtcc
     1441 gggtagcctt cccagtagtt ctgcgcgctt tcgcccggct gcacgcggct gaagtcctgg
     1501 gcgacgttgc tgcaaccgac cgcatacggc cctggcggca acggatcgac gacggccagc
     1561 gagtccgcgt gcccgctgcc accgatacac aacgagagca gcagaacggc cgccgccgca
     1621 cccccacgag gcgaaatgaa tgaagttgtg gatctcaagc cgtgtctccg ggtcggttta
     1681 gtggaggtag accgcgtcgc ctgtccggcg ttccactagc ggtcaggacc cgagaccgat
     1741 gctgcgacta cccgatccaa gctaccattt cgccgggccc cacgtttggc ttatagccgc
     1801 aatgtcacgc aagagccgca accgacctaa agcgacgcgc ccggcagagc cctttgcttc
     1861 ccggcctcca gcagcgacga cgccacccga atcgcgcgat gcactgctgc tgcaagcggc
     1921 actcgcgtac caggcgcagc ggctgcgcga tgcggaggcg ctttgcgtcg acgtccttgc
     1981 cctggaaccc cgctatccgc cggcaatgca gatgctggcg ataattgcct cccgaaccgg
     2041 acggccggca atggccatca aactccttag ggacgtcgtt ttgctcgacc gccgatcagt
     2101 cgatgcacgc aacgagcttg gcgcgttgct ccgtggcgac ctccattttt cggaagcgat
     2161 agccgtcggg ctggaagcgg tccggc
//
LOCUS       DATANS010000203         2621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_807527, whole genome shotgun sequence.
ACCESSION   DATANS010000203 DATANS010000000
VERSION     DATANS010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807527"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /gene="leuB"
                     /locus_tag="VHT22_02915"
     CDS             complement(<1..1044)
                     /gene="leuB"
                     /locus_tag="VHT22_02915"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX3136533.1"
                     /translation="MKIAIFPGDGIGVEIVAEAVRVLERLRSEGLDLAIEGAPIGGAG
                     YDSSGQPLPDATLELARRADAIVLGAVGGPRYDVLPRALRPEQGILALRKALGLFANL
                     RPAILYPELADASTLKPEVVAGLDLMIVRELTGDIYFGLPRGRRSNASGESEGFDTMV
                     YSEPEIRRIARVGFELARKRARKLCSVDKANVLDTSILWREVVTAVGAEYTDVELSHM
                     YVDNAAMQLVRNPKQFDVIVTGNIFGDILSDEASMLAGSIGMLPSASLDANGKGLYEP
                     IHGSAPDIAGQDKANPLATILSLAMMFRHSFGRADVAARIEGAVRKVLAQGLRTRDIA
                     LPGERVVGTRAMGD"
     gene            complement(1136..1774)
                     /gene="leuD"
                     /locus_tag="VHT22_02920"
     CDS             complement(1136..1774)
                     /gene="leuD"
                     /locus_tag="VHT22_02920"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX3136534.1"
                     /translation="MQPFRILTGLVAPLDRANVDTDAIIPKQFLKSVKRSGFGANLFD
                     SWRYLDKGEAGSDHSKRPVNPDFALNQPRYRGAQILLARRNFGCGSSREHAPWALADY
                     GFRALIAPSYADIFFNNCYKNGLLPIVLSESEVARLFDDVAAFPGFRLTIDLERQTVA
                     TPDGSATFKFEVEPFRKHCLLNGLDEIGLTLTHAGEIAAFEAKRKIEQPWLF"
     gene            complement(1785..>2621)
                     /locus_tag="VHT22_02925"
     CDS             complement(1785..>2621)
                     /locus_tag="VHT22_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX3136535.1"
                     /translation="KLGVGGATGHVIEYAGAAIAALGMEERMTVCNMSIEAGARAGMI
                     AVDDKTIAYLRGLPFAPSGEMWERAVAYWRTLKSDASAKFDRVVAVDAATIAPQVTWG
                     TSPEMVTTIEGRVPDPDKERDPMRRDGIERALLYMGLVPNTPMTDIRIDKVFIGSCTN
                     SRIEDLRAAAAVVSGRHKADNVKLAMVVPGSGLVKAQAEREGLDRVFTAAGFEWREPG
                     CSMCLAMNEDRLEPGERCASTSNRNFEGRQGTGGRTHLVSPAMAAAAAIAGHFVDVRR
                     HS"
BASE COUNT          433 a          902 c          835 g          451 t
ORIGIN      
        1 gtctcccatt gcccgcgttc ccacgacacg ctcgcccggc aacgcgatgt cgcgcgttcg
       61 caagccctgc gcgagcacct tgcgcaccgc gccctcgatt cgcgcggcga cgtcggcgcg
      121 gccgaagctg tggcgaaaca tcatcgccag cgacaggatc gtcgccagcg ggttggcctt
      181 gtcctgcccc gcgatgtccg gcgccgatcc gtggatcggc tcgtagagtc ccttgccgtt
      241 cgcatccagc gaagcggacg gcagcatgcc gatagagccg gcgagcatcg aggcctcgtc
      301 ggacaggatg tcgccgaaaa tattgccggt cacgatgacg tcgaactgct ttggattgcg
      361 caccagctgc atcgccgcgt tgtcgacgta catgtgcgaa agctcgacgt cggtgtattc
      421 cgcaccaacc gccgttacga cctcgcgcca cagaatcgac gtatcgagga cgttcgcctt
      481 gtcgaccgag cacagcttgc gagcgcgctt gcgcgccagc tcgaagccga cgcgcgcgat
      541 ccgccggatc tccggctcgc tgtagaccat cgtgtcgaac ccttccgact cgcctgaggc
      601 attgctgcgg cgtccgcgcg gcaggccgaa gtagatatcg ccggtcaact cgcgcactat
      661 catcaggtcg agcccggcga ccacctcggg tttcagcgtc gacgcgtccg cgagctccgg
      721 gtaaaggatc gccggacgca agttcgcgaa caagccgagc gccttgcgca gcgctaggat
      781 tccctgctcg gggcgcagcg cgcgcggcaa gacgtcgtag cgcggtccgc cgacggcgcc
      841 gagcacgatc gcgtcggcgc gccgcgccag ctcgagcgtc gcatccggca gcggctgccc
      901 ggacgaatcg taaccggcgc cgccgatagg cgcgccttcg atcgccaggt cgagtccctc
      961 ggagcgcagt cgctcgagga cccgcacggc ctcggcgacg atttcgacgc cgataccatc
     1021 gccgggaaaa atcgcgatct tcaccgcgac ctccggtggc ggcagcgaag agcgcgagga
     1081 gcagcttcta gtggtccgct tggctccgtc gtcgtctctt catccggttt tccggtcaga
     1141 agagccacgg ttgctcgatt tttcgcttgg cctcgaacgc agcgatttcg ccggcgtgcg
     1201 tcaaggtcaa gccgatctcg tcgaggccgt tgagcaggca gtgcttgcga aacggctcga
     1261 cctcgaactt gaacgtcgcg ctgccatcgg gcgttgccac cgtctgccgc tcgagatcga
     1321 tcgtcagcct gaatccggga aacgccgcga catcgtcgaa tagtcgcgcg acctcgcttt
     1381 ccgacaggac gatcggcagc aagccgttct tgtagcagtt gttgaaaaag atatcggcat
     1441 acgaaggtgc gatcagcgcg cgaaacccat agtcggcgag cgcccagggc gcgtgctcgc
     1501 gcgagctgcc gcagccgaaa ttgcggcgcg cgagcaggat ctgcgcgccg cggtatcgcg
     1561 gctgattgag cgcgaaatcc ggattgaccg ggcgcttcga gtgatcgctg ccggcctcac
     1621 ccttgtccag atagcgccag gagtcgaaca ggttcgcgcc gaatcccgag cgtttgaccg
     1681 acttcagaaa ctgcttcggg atgatcgcgt cggtgtcgac gttggcgcga tcgagcggcg
     1741 ccaccagccc ggtcaggatg cgaaacggct gcatctgtcc accttcagga atgtcgacgc
     1801 acgtcgacga aatgtccggc gatcgctgcg gcggcagcca tcgccggact gaccagatgc
     1861 gtgcggcctc cggtaccctg cctgccttcg aaattgcggt tcgaggtcga tgcgcagcgc
     1921 tcgccaggct ccaggcggtc ctcgttcatc gccaggcaca tcgagcaccc gggctcgcgc
     1981 cattcgaagc cggccgccgt gaacacgcgg tcgaggcctt ctcgctcggc ttgcgccttg
     2041 accagtccgg agcccggcac gaccatcgcc agcttgacgt tgtccgcctt gtgccggccg
     2101 ctgaccaccg ccgcggccgc gcgaaggtcc tcgatgcgcg aattcgtgca cgaaccgatg
     2161 aataccttgt cgatgcgaat gtcggtcatc ggcgtgttcg ggacgagtcc catgtacagc
     2221 agcgcgcgct cgatgccgtc gcggcgcatc ggatcgcgct ccttgtccgg gtccggcacg
     2281 cggccttcga tcgtcgtcac catttccggc gacgtgcccc aggtcacctg cggagcgatc
     2341 gtcgccgcat cgacggcgac gacgcggtcg aacttcgcgc tcgcgtcgga cttgagcgta
     2401 cgccagtacg cgacggcgcg ttcccacatc tcgccagacg gtgcgaacgg gagaccgcgc
     2461 agatacgcaa tcgtcttgtc atcgacggcg atcatcccgg cgcgggcgcc cgcctcgatc
     2521 gacatgttgc acaccgtcat ccgctcttcc atcccgaggg cggcgatcgc cgcgcccgcg
     2581 tactcgatga cgtgcccggt ggcgccgccg acgccgagct t
//
LOCUS       DATANS010000204         1880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_725337, whole genome shotgun sequence.
ACCESSION   DATANS010000204 DATANS010000000
VERSION     DATANS010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725337"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="VHT22_02930"
     CDS             <1..1027
                     /locus_tag="VHT22_02930"
                     /inference="COORDINATES: protein motif:HMM:NF021624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEX3136536.1"
                     /translation="VREAMLYHTRDVEDARARVAKARATLDFLAEAVPDRDSAYGRLL
                     RLAAEHIRQKPDYYVLHEYLEERNEAVYFEQFVERVVARGLRYLGDSDIRTMLPLGLP
                     PKTDATLNRIAPGLMRREQFLDFLKSRSFRQSLLIRAGAALDRKLSQARVMALRIAAP
                     VQRNETPVDLASDRAETFRFADGRSVEASTRIVKSALPILAAQWPLAIPFDQLLQQAA
                     EKIGVHDAPAERDVLAAEILRLHLGGLAELNRAAPPFVLVSGPRPQASAIARRDAPRG
                     AASANLRHEPIVLRDDERALLPLLDGTRTREQIAASRWPALPEAERLAALDAALALLG
                     RQALLVL"
     gene            complement(1070..>1880)
                     /locus_tag="VHT22_02935"
     CDS             complement(1070..>1880)
                     /locus_tag="VHT22_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3136537.1"
                     /translation="PPLTPAELLEISMIQSVGGQLAEGTLTDRRPFRAPHHSASMAAL
                     VGGGIQARPGEVSLAHHGVLFLDELPEWDRRVLEVLREPIESGRIHISRAARQATFPA
                     RFQFVAAMNPCPCGYLGHAGGRCRCTPDQVARYRARISGPLLDRIDLQIEVPSLAADA
                     MPGPHRAAISGETSAAVRERVAAARHRQHRRQAKANAWLDVKEVERWCVPNSDGDALL
                     KSAFARLALSARAYHRILKVARTIADLADSDALAGAHVAEAIGYRRFDRIA"
BASE COUNT          291 a          659 c          645 g          285 t
ORIGIN      
        1 cgtgcgcgag gcgatgctct atcacacgcg cgatgtcgag gatgcgcggg cgcgggtcgc
       61 gaaggcccgc gcgacgctcg acttcctggc cgaagccgtg ccggaccgag actcggccta
      121 cggccggctg ctgcgtctgg ctgccgagca catccggcaa aagcccgact attacgtgct
      181 gcacgaatac ctcgaggaga gaaacgaggc ggtgtatttc gagcaattcg tcgagcgtgt
      241 cgtcgctcgg ggcctgcgct acctcggcga tagcgacatc cgaacgatgc tgccgctcgg
      301 ccttccgccc aagaccgatg cgacgctgaa ccggatcgcg ccgggattga tgcgccgcga
      361 gcagtttctt gattttctga agagtcgcag ctttcgccaa agtctgttga tccgcgctgg
      421 cgcggcgctc gatcgcaagc tctcgcaggc ccgagtgatg gcgctgcgta tcgcggcgcc
      481 cgtgcagcgc aacgagacgc cggtcgacct cgcctcggat cgcgccgaga cgtttcgctt
      541 tgcggacgga cgaagcgtcg aggcgtccac ccgcatcgtc aagtccgcac tgccgatact
      601 ggccgcgcaa tggccgctcg caattccctt cgaccaattg ctccaacagg cggcggagaa
      661 gatcggcgtg cacgacgcgc cggccgagcg cgatgttctg gcagccgaga tcctgcgcct
      721 gcacctgggc ggcctggcgg aattgaatcg cgcagccccg cccttcgtgc tggtctcagg
      781 accgcggccc caggcgagcg ccatcgccag gcgcgacgcg ccgcgcggtg cggcaagtgc
      841 gaacctgcga cacgaaccga tcgtcttgcg ggacgacgag cgcgcgttgc tgccgctgct
      901 cgacggcacc cgcacgcgcg aacagatcgc cgcgtcgcgc tggccggcgc tgcccgaggc
      961 ggaacgcctg gcggcgctcg acgcggcgct cgccttgctg gggcggcagg cgctgctggt
     1021 gctctgagtg cgcgcttcgc cgccgcccgg atcatcgtcg ttcgcgcgtt cacgctatcc
     1081 gatcgaaccg ccggtagccg atcgcctcgg cgacatgcgc tcccgccagc gcgtcgctgt
     1141 cggcaagatc ggcaattgtg cgcgccacct tgagaatccg gtgataggcg cgcgccgata
     1201 gcgcgagccg tgcgaacgcg cttttcagca gcgcatcgcc atcggaattc gggacgcacc
     1261 agcgctcgac ttccttgacg tccagccatg cgttcgcttt cgcctggcga cgatgctgac
     1321 ggtgcctcgc cgcggcgacg cgctcgcgca cggcggcgct cgtctccccg gagatggccg
     1381 cccgatgcgg tcccggcatc gcgtcggctg cgagcgacgg cacctcgatc tgaagatcga
     1441 tgcggtcgag cagcggtccg gaaattcgcg cgcgataacg cgcgacctga tccggcgtgc
     1501 agcggcatct gccgccggcg tggcccagat agccgcacgg gcacggattc atcgcggcga
     1561 cgaactggaa gcgggccgga aacgtcgcct ggcgcgccgc gcgcgagata tggatgcggc
     1621 ccgactcgat cggctcgcga agcacttcga gcacccggcg gtcccactcc ggcaactcgt
     1681 cgaggaacag cacgccgtga tgggcgagcg aaacctcgcc cggccgcgcc tggatgccgc
     1741 cgcccacgag cgcggccatc gaggcggaat ggtgcggcgc gcggaacggg cggcggtccg
     1801 tcaacgtgcc ctcggccagt tgcccgccca ccgactggat catcgagatc tcaagcagct
     1861 cggccggcgt caggggcggc
//
LOCUS       DATANS010000205         2369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_53226, whole genome shotgun sequence.
ACCESSION   DATANS010000205 DATANS010000000
VERSION     DATANS010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53226"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="VHT22_02940"
     CDS             <1..1139
                     /locus_tag="VHT22_02940"
                     /inference="COORDINATES: protein motif:HMM:NF017388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HEX3136538.1"
                     /translation="LGKGGKSYYALDISDPASITDETTAAQKVLWQFTDADMGYSYGR
                     PVIAKTHAFGGKWVVFVTAGYNNASGKGKIFILDAKDGSLLKTLSTGVGSATTPSGLA
                     QIAGYEKDFRNQIAEQIYGGDLLGNFWRFDISGTDETAASWGAPVKLANLTAPDGTPQ
                     AVTIAPQIEIDIANGIDRWVFIGTGRLLDDLDLTTASIANQVQTFYAIRDGTAATPQT
                     PWGAALAPRTDMDLITAALGITGLNNKPAKGWFDDLPVGQRIVVPVQAALSLVAYVGT
                     SPQTDPCLTGQPATVYAREYARGNSLLLDTDGVTPIESVFAAEGGVGVELVALDGANG
                     GGTGGYPDLRIAITAGTTGKVTFYKPKNPDFSQKHRMSWRLLGQ"
     gene            1361..2005
                     /locus_tag="VHT22_02945"
     CDS             1361..2005
                     /locus_tag="VHT22_02945"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX3136539.1"
                     /translation="MTLSPNLLGLTGTLQVQLREETMPVIEPMPEPMAPITVPRSLPT
                     PEAISLEALIRNPPVPASAASGTVTTQPLVVREGPPNPNGAVAVGPLANPERVSAATA
                     SRLAQRFPVRAARPPLPRGAVITGYPLEAARSHLSARIAAVLTIDAAGKVVPADTKLV
                     PDDPMFHSAVMAAIDGAVFTPAELDGDPMPYWLILEFVFRIDPPAGNPAAAGPR"
     gene            complement(2009..>2369)
                     /locus_tag="VHT22_02950"
     CDS             complement(2009..>2369)
                     /locus_tag="VHT22_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018831061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX3136540.1"
                     /translation="YREEIFGPVLSIVRTESLDAAIELINANPYANGAALFTRSGFAA
                     RKFQQDVEVGMIGVNVPIPVPMAFYSFGGWRNSLFGDLHVHGMDGVRFYTRGKAVTAR
                     WPDDGSAAPGFHMPTMG"
BASE COUNT          433 a          802 c          759 g          375 t
ORIGIN      
        1 gcctcggcaa gggcggcaag tcctactacg cactggatat atcggacccc gcgagcatca
       61 ccgacgaaac caccgcggcg cagaaggtgc tgtggcaatt caccgatgcc gacatgggct
      121 acagctacgg ccggccggtc atcgccaaga cgcatgcctt tggcgggaaa tgggtcgtgt
      181 tcgtcacggc gggctacaac aacgcgagcg gcaagggcaa gatcttcatc ctcgacgcca
      241 aggacggatc gctgctgaag accttgtcga cgggcgtcgg aagcgcgacg acaccttcgg
      301 gtctcgcgca gatcgccggg tacgaaaagg acttccgcaa tcagatcgcc gagcagatct
      361 acggcggcga cctgctgggg aatttctggc gcttcgacat ctcgggtacc gatgaaacgg
      421 cggcgagctg gggcgcaccc gtcaagctcg cgaacctgac cgctccggac ggcacgccgc
      481 aggccgtgac gattgcgccg caaatcgaga tcgacatcgc caatggcatc gaccgctggg
      541 tgttcatcgg aaccggcaga ttgcttgacg acttggatct gacgactgct tcgatcgcca
      601 atcaggtgca gacgttctac gcgatccgcg acggcacggc ggcgacaccg cagacgccgt
      661 ggggcgcggc actcgcgcca cgcaccgaca tggacttgat caccgccgcg ctcggcatca
      721 ctggcctgaa caacaagccg gccaagggct ggttcgacga tctgccggtc ggacagcgca
      781 tcgtcgttcc ggtgcaggcc gccctgtcgc tggtcgccta cgtcggcacg tcgccgcaga
      841 ccgatccctg cctgaccggc cagccggcaa cggtgtacgc gagggaatac gcgcgcggca
      901 attcgctgct gctggacact gacggcgtta cgccgatcga aagcgtcttc gcggcagagg
      961 gtggcgtcgg tgtcgagttg gtcgcgctgg atggagccaa cggagggggc accggcggct
     1021 atcccgattt gcggatcgcc atcaccgcgg gaacgaccgg caaggtgacc ttctacaagc
     1081 cgaagaatcc cgacttctcg caaaagcatc ggatgtcgtg gcgcttgctc ggccagtaga
     1141 catcgcacat catcgcggct gaacgcggcc gctccgtgca agcggagcgg ccgcgtcgtt
     1201 ttgcaacctt accctcgccc gcccgatgag ctccgcaaca atttatcgtc cggcctatca
     1261 ggcgatgccg gggcccgaac ggcgattcgg ctattcggtc gcggcatcga gcgtcgtgca
     1321 cggcctgctg atcatcgcgc tcgccagcgt ccggccgacg atgacgcttt cgccgaacct
     1381 gctggggctc accggaacgc tgcaggtgca gctccgcgaa gaaaccatgc cggtgatcga
     1441 gccgatgccg gagccgatgg caccgatcac ggtgccgagg tccctgccga ctcccgaggc
     1501 gatctcactg gaagctttga tccgcaaccc gccggtgccg gcaagcgcgg caagcgggac
     1561 ggtgacgacg cagccgttgg ttgttcgcga gggacctcca aaccccaacg gcgcggtcgc
     1621 ggtcggcccc ttggcgaatc ccgaacgggt cagtgccgcc accgcatcgc ggctcgcaca
     1681 gcgcttcccc gttcgcgcgg cgcgaccgcc actgcccaga ggcgcggtga tcaccggcta
     1741 cccgctggaa gcggcgcgtt cacacctgag cgcccgcatc gccgcggtat tgaccatcga
     1801 cgcagcgggc aaggtcgtcc ccgccgacac aaagctagtg cccgacgacc cgatgttcca
     1861 ttccgccgtg atggccgcga tcgacggcgc tgttttcacg ccggccgagc tcgacggcga
     1921 cccgatgccc tactggctga ttctcgagtt cgtgtttcgc atcgacccac ccgccggtaa
     1981 cccggcggcg gccgggccgc gctgacgact agcccatcgt cggcatgtgg aaccccggcg
     2041 ccgcgctgcc gtcatcgggc cagcgggcgg tcacggcctt gccgcgagtg tagaaccgca
     2101 cgccgtccat gccgtgcacg tgcaggtcgc cgaaaagcga attgcgccat ccgccgaacg
     2161 aatagaacgc catcggcacc gggatcggca cgttgacgcc gatcatgccg acttcgacgt
     2221 cctgctggaa ctttcgcgcc gcgaagcccg agcgggtgaa aagtgcggct ccgttggcat
     2281 acggattcgc attgatcagc tcgatggcag cgtcgagcga ctcagtccgc acgatcgaga
     2341 gcaccggtcc gaaaatttcc tcccgataa
//
LOCUS       DATANS010000206         1660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_137107, whole genome shotgun sequence.
ACCESSION   DATANS010000206 DATANS010000000
VERSION     DATANS010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137107"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..1053)
                     /locus_tag="VHT22_02955"
     CDS             complement(235..1053)
                     /locus_tag="VHT22_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3136541.1"
                     /translation="MTEGYSLREVGKLLGLSRSILGRLIDAGFVSPTRGRRREYRFSF
                     RDLVLLRAAQGLTEAKIPSSRILRSLRRLRSQLPQQVPLDGLRIEAVGDAVVVSEGQT
                     QWRPDDGQYVMQFQVASPDGRLAFFGPDEAPASRTEESWFEQGLALERINDEAACQAY
                     RRALAADPAHCSACVNLGRLLHELGRMQEAETVYIEGLASCGADGTLLFNLAVLLEDM
                     QRADDAAAAYRAALAAAPDLADAHFNLARLCEARGLRQDAVRHLSAFRKLTGDR"
     gene            complement(1078..>1660)
                     /locus_tag="VHT22_02960"
     CDS             complement(1078..>1660)
                     /locus_tag="VHT22_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX3136542.1"
                     /translation="YYDKAYFLGPDKGGAKPYALLAESMRQTRQTAVGRYAARGKQYI
                     VQLRAVPGGLVMQQLLYAPEVRALADIGIEDAPVRDTELALAKQLIAQISSKQFDPSA
                     YEDDVKKRIEAAVQKKVEGEEIAISPSAPETGAQVIDLMEALRASLEKTAGKRAPPKS
                     VAAAPPSEIKSAQRKPPKKIATEPETRRKAAKR"
BASE COUNT          258 a          540 c          560 g          302 t
ORIGIN      
        1 ttccagtggc gcttgagact cgccccgccg gcgaagatcc aatcgaattc gacgcgcgtc
       61 gggtgttcgg ccgcagccgc gagatcggac agcagccgcg gcagctggtt gcgcgacgta
      121 tagctgtttc caatgtacag gagccgggtt ggcggcgcgg tggggtccgc tggcatcgtc
      181 gggtggctct tgcggtcctc gatcgcctgt gtccgtgccg cgcaagcctg ctcgttaccg
      241 gtcgccagtc agcttgcgaa aggcgctcaa gtgacgcacc gcgtcctggc gcaatccgcg
      301 cgcctcgcac aggcgcgcca gattgaaatg tgcatccgcg aggtcgggcg ccgcggccag
      361 tgcagcgcgg tacgcggcgg cggcatcgtc ggcccgctgc atgtcttcca gcagcaccgc
      421 gagattgaac aacagcgtgc catccgcgcc gcacgaagcg agtccttcga tgtacacggt
      481 ttcagcttcc tgcatgcggc cgagctcgtg cagcaatctg cccagattga cgcaggcgct
      541 gcaatgcgcg gggtcggcgg cgagcgcgcg gcgatacgct tggcacgctg cctcgtcgtt
      601 gatgcgctcg agggcaagtc cttgctcgaa ccaggactct tcggtccggg acgctggcgc
      661 ttcgtcaggg ccgaaaaagg cgagcctccc gtcgggcgat gcaacctgga attgcatcac
      721 atactgaccg tcgtcgggac gccattgcgt ctggccttcg ctcaccacga cggcgtcgcc
      781 tacggcctcg atccgcaacc cgtccaaagg aacctgctgc ggcaattgcg agcgcaaccg
      841 gcgcagcgaa cgcaggatgc gcgacgacgg aatcttggcc tcggtcaggc cctgggccgc
      901 gcgcagcagc accaggtcgc gaaacgagaa acggtattcg cggcgccggc cgcgagtcgg
      961 cgatacgaag ccagcgtcga tgagccgacc gaggatggaa cgggaaagtc cgagcagctt
     1021 gccgacttcg cggagcgaat agccttcggt cacgacggca cgggcatcga tcgtcgccta
     1081 gcgcttggcg gccttgcggc gcgtctccgg ctcggtggcg attttcttcg gcggcttgcg
     1141 ctgcgcgctc ttgatttcgg acggcggcgc ggcagcaacg gatttcggcg gcgcgcgctt
     1201 gccggcggtc ttttccagac tggcgcgcag cgcttccatc aggtcgatca cctgggcgcc
     1261 cgtttccggc gcgctgggcg aaatcgcgat ttcctcgccc tcgacctttt tctgaaccgc
     1321 ggcctcgata cgcttcttta cgtcgtcttc gtatgcgctg ggatcgaact gcttcgatga
     1381 gatctgcgcg atcagctgct tggccaatgc gagctcggta tcgcgcactg gagcgtcctc
     1441 gatgccgata tcggcgaggg cgcgcacttc cggcgcatac agaagctgct gcatcaccag
     1501 gccgcccggc acggcgcgca actggacgat gtactgcttg ccgcgcgcgg cgtagcgccc
     1561 gacggcggtc tggcgggtct ggcgcatgct ttcggccagc agcgcatagg gtttggcgcc
     1621 ccccttgtcg ggtccgagga agtaggcctt gtcgtaatag
//
LOCUS       DATANS010000207         2579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_263532, whole genome shotgun sequence.
ACCESSION   DATANS010000207 DATANS010000000
VERSION     DATANS010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263532"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1896
                     /locus_tag="VHT22_02965"
     CDS             <1..1896
                     /locus_tag="VHT22_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX3136543.1"
                     /translation="KTRVITAKIAHLIEQGADPAGIAAITFTNKAAREMRERSGAAQR
                     RRETAPAAPTICTFHALGLKILRREAAAMQLTPRFSILDPADVEPIVAELLTTSDRAR
                     VRAAQWQISAWKNALIAPPEALARASTEAERAAARVYARYRAALSAYQAVDFDDLIAL
                     PVAALECDAGAAEHWQKRLQYLLIDEYQDTNPAQYRLLRLLTGARAAFTAVGDDDQAI
                     YGWRGATVANLERLPRDYPSLKVIKLEQNYRSSIRILRSANALIGHNTKLFDKALWSD
                     LGLGEPIQVAPAADDQAEAENVARTIAALKFEHGTRFDDFAVLYRGNHQARAFEQALR
                     AQNMPYSVSGGQSWFDRTEIRDIVAYLRLIANDEDDPAFVRAVTTPRRGVGAQTLEHL
                     GRAGGLFAAACDDGLTSALPARQREALTEFCRMINGLRYRAEREPAGRLIDELIKTIG
                     YEDHLCSTCDKREAQTRWESVVDFVRWLSTKGESDGRNLLELTQSIALLTLLDGEEGR
                     EPDAVRLSTLHAAKGLEFAHVFLVGLEEGVLPHREAIVAGNIDEERRLMYVGLTRAQQ
                     SLRLSWCRRRKRAGEWHGAEPSRFIGELAQEDLRHAEAALEPADAAREKEAGNARLRQ
                     LRAMLTR"
     gene            complement(1901..>2579)
                     /locus_tag="VHT22_02970"
     CDS             complement(1901..>2579)
                     /locus_tag="VHT22_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136544.1"
                     /translation="PRADVLFVHIPKTAGISLYTAMAKEFGPEHSLRYPRSTEAFKQQ
                     FLRLSEEELHRYRLISGHFDLPFWLQRDLGGRVIVALVREPIERALSTYRYTMSFTGH
                     PRHAMVRNMGIAGFVDHYVAETARHNRQCRSLCGSGDFLAAREMARKQIDLLGSVEAI
                     AMLTKDLGERLGVPLDLGVENRSTLEHPKRSDLDAALLARLESCNVEDRKLWAYVNER
                     GLVRGHD"
BASE COUNT          471 a          828 c          847 g          433 t
ORIGIN      
        1 aagacgcggg tgatcaccgc caagatagcg cacctgatcg agcagggcgc cgatccggcc
       61 ggcatcgcgg cgatcacctt taccaacaag gccgcgcgcg aaatgcgcga gcgaagcggc
      121 gcggcacaac gccgacgcga aaccgcgccc gctgcgccga cgatatgcac gtttcacgcc
      181 ctgggcttga agattctgcg tcgcgaagcc gccgcaatgc agctgacgcc gcggttttcg
      241 attctcgatc ctgccgacgt cgagccgatc gtcgccgagc tgctgaccac gagcgaccga
      301 gcgcgcgtgc gcgccgcgca gtggcagatc agcgcgtgga agaatgcgct tatcgcgcct
      361 cccgaggcgc tggcgcgagc ctcgaccgag gcggaacgcg cggcagctcg agtctatgcg
      421 cgctatcgcg ccgctctgtc cgcttatcaa gcggtcgact tcgacgatct gattgcgttg
      481 ccggttgccg cgctcgaatg cgacgccggc gccgcagagc attggcagaa gcgcctccag
      541 tacctgctga tcgacgaata ccaggatacc aacccggcgc aataccggtt gctgcggctg
      601 ctgaccggag cgcgcgcggc attcaccgcg gtcggggacg acgaccaggc gatctatggc
      661 tggcgtggcg cgacggtggc caatctcgag cggctgccgc gcgattatcc atcgctcaaa
      721 gtcatcaagc tcgagcagaa ctatcgctcg tcgatcagga tcctgcgctc ggcgaacgcg
      781 ctgatcgggc acaacaccaa gctcttcgac aaggcgctat ggagcgatct cgggctcggc
      841 gagccgatcc aggtcgcgcc cgctgccgac gaccaggccg aggccgagaa cgtcgcgagg
      901 accatcgccg cgctcaagtt cgagcatggc acccgcttcg atgatttcgc ggtgctctat
      961 cggggaaatc accaggcacg cgcgttcgag caggcgctgc gcgcgcagaa catgccctac
     1021 tcggtcagcg gcggccagtc atggttcgac cgcaccgaga ttcgcgatat cgtcgcctat
     1081 ctgcggctga tcgccaacga tgaagacgat cccgcgttcg tgcgcgcggt gacgacgccg
     1141 cgccgcgggg tcggtgccca gacgctcgag cacctcggtc gcgccggcgg attgtttgcc
     1201 gccgcctgcg atgacgggct caccagtgcg ctgccggcgc ggcaacgcga ggcattgacc
     1261 gagttctgca gaatgatcaa tggtcttcgc tatcgggccg agcgcgagcc ggccggccgg
     1321 ctgatcgacg agctgatcaa aaccatcggt tacgaggacc atctgtgctc gacctgcgac
     1381 aagcgtgaag cgcaaacgcg atgggaaagc gttgtggatt tcgttcgctg gctttccacc
     1441 aagggcgaat ccgatggacg caatcttctc gagctgacgc aatcgatcgc gctgctgaca
     1501 ctgctcgacg gcgaagaggg ccgggaaccg gacgcggtgc gtctgtcgac attgcacgca
     1561 gcgaagggcc tcgaattcgc gcacgttttc ctggtcggac tggaggaagg cgtgctgccg
     1621 catcgcgagg cgatcgttgc gggcaacatc gatgaggaac gccggctcat gtatgtcgga
     1681 ttgacgcgtg cgcagcagag tctgcgtctg tcgtggtgcc ggcgccgcaa gcgagcgggg
     1741 gaatggcacg gcgccgagcc gtcgcgcttc atcggcgagc tcgcccagga ggatttgcgc
     1801 cacgccgaag cggcgctgga gccggccgat gccgcgcgcg agaaggaagc aggcaacgcg
     1861 cgcttgcggc aactgcgcgc tatgctgacg cgctaagagc ctaatcgtgc ccgcggacga
     1921 gcccgcgctc gtttacgtac gcccaaagct tgcggtcctc gacgttgcag gattccaatc
     1981 tggctagcag tgcagcgtcg agatcgctcc tcttcgggtg ctcgagcgtg ctccgatttt
     2041 ccacgcccag atccagcggc acgccgagcc gctcgccgag gtctttggtc aacatcgcga
     2101 tagcttcgac cgagcccagc aggtcgattt gctttcgcgc catttccctt gccgccagaa
     2161 agtcaccgct cccgcaaaga gaacgacatt gacggttgtg gcgggcagtt tctgcgacgt
     2221 agtggtcgac aaacccggcg atccccatgt tgcggaccat cgcgtgccgc gggtggcctg
     2281 taaagctcat cgtatagcga tacgtggaca gggcccgctc gatgggctcc ctcaccaggg
     2341 ccacgatgac acgcccgccc agatcacgct gaagccagaa cggcagatcg aagtgaccgg
     2401 agatcagccg ataccggtgc agttcttcct cggacagtcg caggaactgc tgtttgaacg
     2461 cttcggtgga gcgcgggtat cgcagcgagt gctcgggccc gaactccttg gccatggcgg
     2521 tgtacagcga tatccccgcg gttttcggga tatgcacgaa cagtacgtcg gcgcggggc
//
LOCUS       DATANS010000208         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_641998, whole genome shotgun sequence.
ACCESSION   DATANS010000208 DATANS010000000
VERSION     DATANS010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641998"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VHT22_02975"
     CDS             complement(<1..411)
                     /locus_tag="VHT22_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3136545.1"
                     /translation="MNKLFRSTGPVAVAVLAAALLSGAALAQQKVLKFIPQADLRILD
                     PIATTAYITRNHGYMVYDTLFAMDEKFQVKPQMIDKHDLSKDGLTYTFTLRDGLKFHD
                     GQPVRSADCIASIDRWSKRDALGQKLAEATESWSA"
     gene            complement(601..2079)
                     /locus_tag="VHT22_02980"
     CDS             complement(601..2079)
                     /locus_tag="VHT22_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX3136546.1"
                     /translation="MKVVIARLNHETNTFSPLSTPLAAFGAGDPHGPAFGADAYTAAR
                     GSATAMGAYIDMAERKGCEIVTPLFAMANPSGPVEAAAYRVMCDAIVDAIAAGCDAIM
                     LDLHGAMVVEDNGDGEGALLERIRAIAPATPLALALDLHGNISPRMIGHADIVVGFKT
                     YPHIDMYDTGAHAARLLFDMLAGKTQPVVAVARPGVLSQTLCQNTEIPGAMRDGVEFA
                     RGLERHGALAATVFGGFYLADIEDAGMSVVVVADRDMAKAQRWADDCSEMLAGRRSEF
                     VFGQTPLSDSIARARAVKHGLTLLLDHGDNCMSGGTCDTMDVLDECLRHGMTHIGVGP
                     ICDPQAVAQLIAAGEGATVTIDLGNKVPMPKIAQAPRAPLRLTGQVRAIGNGEYTISG
                     PIYTGTRCYMGRTVLFDIGAARIVVTEQTQEPWDLGVFTCVGLQPTRERYLILKSRMY
                     YRPVFAPLASTIIECESGGVCSSDFSLFEYGRVRRPIYPLDH"
BASE COUNT          394 a          700 c          672 g          401 t
ORIGIN      
        1 cgcgctccag ctctcggtcg cttcggcaag cttctggccg agcgcatcgc gtttggacca
       61 gcggtcgatg gaggcaatgc agtccgcaga gcggaccggc tggccatcgt gaaatttgag
      121 gccatcgcgc aaggtgaacg tatacgtgag gccgtctttc gacaggtcgt gcttgtcgat
      181 catctgcggc ttgacctgga acttctcgtc catggcgaac agcgtgtcgt acaccatgta
      241 accatggttg cgcgtgatgt aagccgtcgt ggcgatcgga tcgagtatgc gcaaatcggc
      301 ctgcggtatg aacttcagga ctttttgctg cgccagcgcg gcgcccgaca gcaacgctgc
      361 ggcaagcacg gctacggcga ccgggccggt cgaacgaaac agcttgttca tcgaaatccc
      421 ctctttcatc aatatatgaa acggatccat cggcgtgagc aatttgaatg cccgcagtct
      481 tgccggacgt gcttattgta ttacatgcct ccgcgccgcc aggcaactat cggtcgactg
      541 cgccggcacg cgcaggactg gggcgtgctg cgccgcgatg gtgcgccacc gcatgacaaa
      601 tcagtggtca agcggataaa tcgggcgccg tacccgcccg tactcgaaca gcgaaaaatc
      661 cgaggaacac acgccgccac tctcgcactc gatgatggtg ctcgcgagcg gggcaaagac
      721 cggccggtaa tacatgcgcg acttgagtat caggtagcgc tcgcgcgtcg gctgcaggcc
      781 cacgcatgtg aagacgccga ggtcccatgg ctcctgcgtc tgctcggtga cgacgatgcg
      841 cgcggcgccg atatcgaata aaaccgtgcg ccccatatag cagcgcgtgc ccgtgtaaat
      901 gggcccgctg atcgtgtact cgccattgcc gatcgcgcga acctgcccgg tcaggcgcag
      961 cggcgcgcgc ggcgcctgtg caatcttcgg catcggcact ttattcccga ggtctatcgt
     1021 gacggtggcc ccctcgcccg ccgcgatcag ctgcgcgact gcctgcggat cgcagatggg
     1081 cccgaccccg atgtgcgtca tgccgtggcg cagacactcg tccagcacat ccatcgtgtc
     1141 gcaggtgccg cccgacatgc agttgtcgcc atgatcgagc agcagcgtca ggccgtgctt
     1201 gacggcgcgc gcccgcgcaa tggagtcgct cagcggcgtc tggccgaata caaattcgct
     1261 gcgccgtccg gcgagcattt cgctgcaatc gtcggcccag cgctgcgcct tggccatatc
     1321 gcggtcggcc acgacgacga cactcatgcc ggcatcttcg atgtcggcca gataaaagcc
     1381 gccgaagacg gtggcggcaa gcgcgccgtg tcgctcgagc ccccgtgcga attcgacacc
     1441 atcgcgcatg gcgcccggta tttcggtgtt ctggcacagc gtctgggaga gcacgcctgg
     1501 tcgcgccacc gcgacgaccg gctgtgtttt tcctgcgagc atgtcgaaga gcagccgcgc
     1561 cgcgtgcgcg ccggtgtcgt acatgtcaat gtgcggataa gtcttgaatc cgaccacgat
     1621 gtccgcgtgc ccgatcatgc gcggcgagat gttgccgtgc aaatcgagcg ccagcgccag
     1681 cggcgtggcc ggcgcgatcg cgcgtatccg ctcgagcaaa gcgccttcgc cgtcgccatt
     1741 gtcttcgacc accatcgcgc cgtgcaggtc gagcatgatt gcatcacacc ccgcggcgat
     1801 cgcgtcgaca attgcatcgc acatcacgcg ataggcggcc gcctcgactg gcccgcttgg
     1861 attggccatt gcaaacagcg gcgtaacgat ctcgcagccc ttgcgctccg ccatatctat
     1921 gtaggcgccc attgcggtgg ccgatccgcg cgcggccgta tacgcatcgg cgccgaacgc
     1981 cggcccatgc ggatcgccgg ccccgaatgc ggcgagcggc gtcgataaag gagaaaacgt
     2041 gttggtctcg tggttcaggc gcgcgatcac gactttcatg gcagcgattc tagcgcgaac
     2101 aaccggttcc gaccctgcat tgccagccga atcgcggcca tgctctaatc ggggagaaat
     2161 ttaattt
//
LOCUS       DATANS010000209         1781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_389445, whole genome shotgun sequence.
ACCESSION   DATANS010000209 DATANS010000000
VERSION     DATANS010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389445"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VHT22_02985"
     CDS             complement(<1..318)
                     /locus_tag="VHT22_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136547.1"
                     /translation="MRGSATRADRRGTTGRGFAPNLAGASTAGAPADTITIAVDTMGG
                     DFGPPVTVAAALAFLAKTPRARVILVGRENEVRQAVAANRTIASDRVIVQAASEVVEM
                     HEPP"
     gene            complement(348..530)
                     /gene="rpmF"
                     /locus_tag="VHT22_02990"
     CDS             complement(348..530)
                     /gene="rpmF"
                     /locus_tag="VHT22_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX3136548.1"
                     /translation="MAVQQNKKSPSKRGMHRAHDFLPKPPLAVEPVSGEVHLRHHISP
                     SGYYRGKKVVKTKADE"
     gene            complement(616..1143)
                     /locus_tag="VHT22_02995"
     CDS             complement(616..1143)
                     /locus_tag="VHT22_02995"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HEX3136549.1"
                     /translation="MATRSTDVGAHGRFDAFELAAAGTDLAGEIDARSLPRAADQLAA
                     GVDAAPITWRISGGHDDAGRPSLTVRLDGVVPLVCQRCLQPFGAAIAQQTDLLLASNE
                     AELARLDAEDTEVVLANTTLDPRNLVEDELLLSLPLSPRHDEEACAAARRSMTDDGKR
                     SPFAGLAALKARNPD"
     gene            1278..>1781
                     /locus_tag="VHT22_03000"
     CDS             1278..>1781
                     /locus_tag="VHT22_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEX3136550.1"
                     /translation="MKTTTSPRNTRALVLASASPYRKALLDRLGLRFTVAAPAIDESP
                     RLDEAPADTAVRLAEAKARHVASAHPGALIVGSDQVADFEGTAIGKPRDPEDALEQLR
                     AMQGRTIVFHTGLALLDARTGRCRRALVDVASTFRQLGDDALKAYLERDRPYDCAGSV
                     KVEALGIA"
BASE COUNT          282 a          609 c          573 g          317 t
ORIGIN      
        1 gggtggctcg tgcatttcga ccacttcgct ggccgcctgg acgatgaccc ggtcgctcgc
       61 gatggtgcgg ttcgcggcta ccgcttgacg cacttcgttc tcgcggccga ccagaatcac
      121 tcttgccctg ggagttttcg ccagaaaggc aagcgcggcg gcgaccgtca ccggcgggcc
      181 gaagtcgcca cccatcgtgt cgacggcgat ggtgatcgtg tcggcgggcg cgccggccgt
      241 tgacgcgccg gcgagattcg gcgcgaagcc ccgacccgtg gtgccgcgcc ggtcggcccg
      301 ggtcgcgctt cctctcatgg tggtgccgga cccgggacgc ctgggtctta ctcgtcggct
      361 ttggtcttga ctactttctt gccgcgataa tacccgctcg ggctgatgtg atggcgaaga
      421 tggacctcgc cgctgaccgg ctccaccgcg agcggcggct tgggtagaaa atcgtgggcg
      481 cgatgcatgc cgcgcttcga aggcgacttc ttgttttgct ggacagccat tgcctgtgac
      541 tccttaaaaa ttcgatcggc acaaaaagcg gtttcccgct tcgcgaaatt cccgtaatcg
      601 tctcgccggc tctgctcaat ccggattgcg cgctttcagc gccgccaatc cggcgaacgg
      661 cgagcgtttt ccgtcgtcgg tcatcgaacg gcgcgccgcc gcgcatgctt cctcgtcatg
      721 gcgcggcgac aaaggcagcg acaacagcag ttcatcctcg accaggttcc gcggatcgag
      781 cgtcgtgttc gccagcacga cctcggtgtc ttccgcatcc agccgagcta actcggcctc
      841 gttgctcgcg agcagcaggt cggtctgctg cgctatcgcc gccccgaatg gctgaaggca
      901 gcgctggcac accagcggca ccacgccgtc gagccgtacc gtcaacgaag gccgtcctgc
      961 atcgtcgtgc ccgccgctaa tccgccacgt aatcggcgcc gcgtcgacgc ctgccgccaa
     1021 ttggtcggcg gcacggggca ggctgcgcgc gtcgatctca cccgccaggt cggttccggc
     1081 ggcggccagt tcgaatgcat cgaagcggcc gtgcgcgccg acgtcagtcg accgagtcgc
     1141 catctggatt ggccttttgg tccggcaaac accgcgtccg gcgacgagtc gctgatcggc
     1201 ggagcatggc ccggccgcta caggcaagga caaacgttgc ataatagcgg gcttgcccct
     1261 gagtgtcaaa gaaaaccgtg aaaacaacaa cttcgcctcg caatacgcga gcgctggtgc
     1321 tcgcctcggc ctcgccgtat cgcaaggcgc tgctcgatcg actgggcctg cggttcacgg
     1381 tcgccgcgcc cgcaatcgac gaatcgccgc ggcttgacga agcgcccgcc gatactgccg
     1441 tccggctggc ggaggccaag gcgcggcacg tggcgagcgc ccatcccggc gcattgatcg
     1501 tcggctccga ccaggtcgct gatttcgaag gcacggccat cggcaaaccg cgcgatcctg
     1561 aggacgctct ggagcagctt cgcgccatgc aagggcggac gatagttttt cacaccggcc
     1621 tggcgctcct cgatgcgcgc accgggcgtt gccgccgcgc actggtcgac gtcgccagca
     1681 cgtttagaca gctcggcgac gacgcgctca aggcctacct cgagcgcgac cgcccgtacg
     1741 attgcgccgg atcggtcaag gtcgaagcgc tcggcatcgc g
//
LOCUS       DATANS010000210         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_597894, whole genome shotgun sequence.
ACCESSION   DATANS010000210 DATANS010000000
VERSION     DATANS010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597894"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /gene="dxr"
                     /locus_tag="VHT22_03005"
     CDS             complement(<1..944)
                     /gene="dxr"
                     /locus_tag="VHT22_03005"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX3136551.1"
                     /translation="MSTSRQLAILGSTGSVGVNTLDVVARHRDRFNVVALAAYRQVDL
                     LYRQCLDFQPLYAVIGSARDAKTLQGRLAAHGSATTVLHGERALEEISSLPEVDTVMA
                     AIVGIAGLRASFAAVKAGKRVLLANKEALVTAGPVFMQAVRAHGATLLPIDSEHNAVF
                     QALPRTFAGDLAASGVRRIVLTASGGPFLNVSREHLHDVTPDQACAHPNWVMGRKISV
                     DSATMMNKGLEIIEASWLFNAAESVIKVVIHPESVIHSLVEYVDGSLLAQLGNPDMRT
                     PIAFALGYPDRIEAGVEGLNLAALRQLNFEEPDSARFP"
     gene            complement(941..>1545)
                     /locus_tag="VHT22_03010"
     CDS             complement(941..>1545)
                     /locus_tag="VHT22_03010"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX3136552.1"
                     /translation="TAAIGLLMAVATTPESASDRIILGLSVAFWLIVAPCWLWLKIVV
                     RNRWWLVICAVVVLVPTWLAFAQLQRTPLLLLLLLAVVWIADTAAYVFGRTFGRHKLA
                     PAISPGKTWEGVTGALATVAVYALALNLAQISGRDGGFLVAAFLSMTVFSVVGDLFES
                     WMKRSAGVKDSGAILPGHGGLLDRIDGVMAALPLAALIFI"
BASE COUNT          310 a          503 c          477 g          255 t
ORIGIN      
        1 cacggaaacc gcgcgctgtc gggctcctcg aagttcaact ggcgcaacgc cgcaagatta
       61 agtccctcga cgccggcctc gatgcggtcc ggatagccga gcgcaaatgc gatcggcgtg
      121 cgcatgtcgg gatttccgag ctgcgcgagc aacgagccgt cgacgtactc gaccagcgaa
      181 tgaatcacgc tctccggatg aatgacgacc ttgatcacgc tttcagcggc gttgaagagc
      241 cagctcgctt cgatgatctc cagccccttg ttcatcatgg tggctgaatc gaccgaaatt
      301 ttgcgcccca ttacccagtt cggatgcgcg catgcctgat cgggcgtcac gtcgtggagg
      361 tgctcgcgcg agacgttgag gaaaggcccg ccggaagccg tcagcacgat acggcgcacg
      421 ccagaagccg caaggtcgcc ggcaaacgtg cgcggtaacg cctggaatac cgcgttgtgc
      481 tcgctgtcga tcggcagcag ggtcgcgcca tgcgcgcgca ccgcctgcat gaatacgggt
      541 cccgctgtga ccagcgcttc tttgttcgcg agcaaaaccc gcttgcccgc cttcaccgcc
      601 gcgaagctcg cgcgcaatcc ggcgataccg acgatggcgg ccatcaccgt atcgacctcg
      661 ggcaaactcg aaatttcctc cagcgcacgc tcgccgtgca acaccgtagt cgcgctgccg
      721 tgggcggcga gccggccctg tagcgttttc gcatcgcgcg cgctgccgat gacggcatac
      781 agcggctgga agtcgaggca ttgccggtac aggagatcga cttgccgata ggcggcgagt
      841 gcgacgacgt tgaaacgatc gcgatggcgc gccacgacgt cgagcgtgtt gacgcccacg
      901 ctgccggtcg agccgaggat tgcgagttgc cgcgaggtgc tcatatgaaa atcagcgcgg
      961 cgagcggcaa cgccgccatg acgccgtcga tgcggtcgag caagccgccg tgaccgggca
     1021 gaatggcgcc gctgtccttg acgccggcgc tgcgcttcat ccatgactcg aagagatcgc
     1081 cgactacgct gaataccgtc atgcttaaaa atgcggcgac cagaaatccg ccatcacgcc
     1141 ccgaaatttg cgccagattc agagccagag catacaccgc gaccgtcgcc agcgcaccgg
     1201 tcacgccctc ccacgttttg ccgggactga tcgccggtgc gagcttgtgg cgcccgaacg
     1261 tgcggccgaa tacataggct gcggtgtcgg cgatccatac cacggcgagc agcaacaaga
     1321 gcagcagtgg agtgcgctgc agctgcgcga acgcgagcca ggttggcacc aatacgacaa
     1381 ctgcgcaaat gacgagccac cagcgatttc gcacgacgat ttttagccac agccagcacg
     1441 gcgccacgat gagccagaac gctacgctta atccgagaat gatgcggtcg ctcgcgcttt
     1501 ccggggtcgt cgcaactgcc atcagcaggc cgatcgcggc cgtca
//
LOCUS       DATANS010000211         5233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_766655, whole genome shotgun sequence.
ACCESSION   DATANS010000211 DATANS010000000
VERSION     DATANS010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766655"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2719)
                     /locus_tag="VHT22_03015"
     CDS             complement(<1..2719)
                     /locus_tag="VHT22_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000716 - transcription-coupled
                     nucleotide-excision repair, DNA damage recognition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX3136553.1"
                     /translation="MPAAPPPGRKAIAQPFAGSADALALAQAATDAAQRRQLLAVVCA
                     DALAAQRLAVEMPWFAPALKVAVLPDWETLPYDHFSPHQDLVSERLATLYRMSRGDCD
                     VVIVAATTALYRFAPPSYLAAFTFFIEQGETLDVDRLRSQLALAGYQHVTQVVSPGEF
                     GIRGGLIDLFPMGSPLPYRIDLFDNQIESIRTFDVDTQRTVYPVPQVRLLPAREFPQD
                     DAGRTRFRGRFREVFEGDPSKSPLYKDVSNGIMPAGIEYYLPLFFDSTATLTEYLPAE
                     AIVCLHGDIPSAIRRFWEDTDARYRLLRGDRARPLLAPSELFVPEDEFHRMLKPFARI
                     ELASDGVSGSGEYAASAENATEPPVVETDADAAAPAAGTVAARAKGSRRGHATHSLPA
                     VQVDRRADDPLRALKRHVTSTPERVLIAAESLGRRETMQQYFAEYGFKPAVVADWAEF
                     IAGDASPALVASPLHAGFVWPGANMTLITEAELYADVVRRPGKRDARRSNVDAMLRDL
                     SEVRIGDPVVHEQHGIGRYLGLTTLDLGDGPNEFLHLLYANDAKLYVPVSSLHLISRY
                     SGASPDAAPLHELGSGQWDKAKKKAAQRAHDTAAELLNLYAQRAARQGHAFKYDLHEY
                     EAFAAGFGFEETPDQAAAIEAVIRDLTSGKPMDRLVCGDVGFGKTEVALRAAFVAVAD
                     GKQVAVLVPTTLLVEQHFQTFSDRFGDLPVKLAELSRFRSAAEMKTVLEGLASGRIDL
                     VIGTHKLIQRDVKFKRLGLVVIDEEHRFGVRQKEQLKRLRAEVDVLTLTATPIPRTLA
                     MSLEGLRDFSVIATAPERRLSIKTFVSQYSPGIVREACLRELKRGGQIYFLHNDIDTI
                     ATMGQKLEALMPEARIAIAHGQMPERDLERVMRDFYQQRSNLLLCSTIIE"
     gene            2828..3727
                     /locus_tag="VHT22_03020"
     CDS             2828..3727
                     /locus_tag="VHT22_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136554.1"
                     /translation="MAPLRTLMRPVGDNAVMRRFNVLVVQPNDYVNYAPFLGVAKSMY
                     FALRRLGYDVRLAGNEFLRDATNVIFGAHHLAGEIAADLPSDTIVYNTEQLRADGALV
                     EALTPFVSRFATWEPDSGNVEAWRALGLGSRVHYVQPGYLPESSTVAAETSTDIDVLF
                     FGKVNPRRMVILDSIMRAGISLHVADGVYLADRDALVARAKILLNVHAADDSALEMAR
                     VAYALANRRALVTELGGGARLDPDLRDGVLAGTNAELAAMCRTLLADDKRRLELAERG
                     FQAFSRRDLVATVRNALAQASAG"
     gene            complement(3772..5094)
                     /locus_tag="VHT22_03025"
     CDS             complement(3772..5094)
                     /locus_tag="VHT22_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX3136555.1"
                     /translation="MSRLPDASLAAPALVLSANDSLTGAVPHLQAAAGAAHGPVLRSN
                     VPFGPDAGPCITLVGSAAAETALLSRRGEFSSEQGWRHVLGVGCGLAVLNTDDPLHAQ
                     QRRTWSPAFAGAILRSYEPAMARIVEDCILAWRDGDELDLYPPMRAFAFRAVAATIGG
                     LPEAAIGPAYHAICTILDGQDYSRESRDAYFERAAAARAILADTLRTAIRERRRRRPA
                     APASLLDVLLAEPGLAGNDAEIQSHLTILLIAGHETGASLYSRALYVLAQMPRLAATL
                     AAELDGASSTRAQPLGADDLDRLPQLDRFMLEVGRLYPPLVNLPRVAASPIEFSGYCI
                     SPGTRIAVAVAATHLLPDNYPDPLRFDIDRYKCGDSASRTRPFVLLTFAGGARMCLGI
                     RFAQMEFKAIVARVLQTMSLSAVGGDVAHAGFWNARPAGPMRALLRAR"
BASE COUNT          921 a         1697 c         1775 g          840 t
ORIGIN      
        1 tctcgataat cgtcgagcaa agcaggaggt tggaccgctg ctgataaaag tcgcgcatca
       61 cccgctcgag atcgcgctcc ggcatctggc cgtgcgcgat cgcgatgcgc gcctcgggca
      121 tcaatgcctc gagcttctgc cccatcgtcg cgatcgtgtc gatgtcgttg tgcaggaaat
      181 agatctgtcc gccgcgcttg agctcgcgca ggcaggcttc gcgcacgatc cccggcgagt
      241 attgcgacac gaaggtcttg atcgacaagc gccgctctgg cgcggtcgcg atcaccgaaa
      301 agtcgcgcag cccttccagc gacatcgcca gcgtccgcgg aatcggcgtc gccgtcagcg
      361 tcagcacgtc gacctcggcg cgcaggcgct tcagctgttc tttctgtctg acgccaaagc
      421 ggtgctcctc gtcgatcacc acaaggccga ggcgcttgaa tttcacgtcg cgctggatca
      481 gcttgtgcgt gccgatgacc agatcgatgc gtccactggc aaggccctcg agtaccgtct
      541 tcatctcggc agccgagcgg aagcgcgaca attcggcgag cttcaccggc aagtcgccga
      601 aacggtcgga gaaggtctgg aagtgctgct cgaccagcag cgtcgtcggc acgagcaccg
      661 cgacctgctt gccatccgcg accgcgacga atgccgcgcg cagcgccact tcggtcttgc
      721 cgaacccgac gtccccgcag accagccggt ccatcggctt gccggacgtg aggtcgcgga
      781 tcaccgcctc gatcgccgcc gcttggtcgg gtgtctcctc gaaaccaaag cccgcggcga
      841 atgcctcgta ttcgtgcagg tcgtacttga acgcgtgtcc ctgcctggcg gcgcgctgcg
      901 catagaggtt cagcagctcg gccgcagtgt cgtgtgcgcg ctgcgccgcc tttttcttcg
      961 ccttgtccca ctgtccgctg ccgagctcgt gcagcggcgc cgcatcgggc gacgctccgc
     1021 tgtagcggct gatcagatgc aggctggata ccggcacata aagcttggcg tcgttcgcgt
     1081 acagcaggtg caggaattcg ttcggtccat cgccgaggtc gagtgtcgtg agccccaggt
     1141 agcggccgat gccgtgttgc tcgtgcacga ccggatcgcc gatgcgcact tccgacagat
     1201 cgcgcagcat cgcatcgacg ttgctgcgcc gcgcatcccg cttgcccggt ctgcgcacga
     1261 catccgcata aagctcggcc tcggtgatca gcgtcatgtt cgcgccgggc cagacgaaac
     1321 cggcgtgcag aggactcgcg accagcgccg ggctcgcgtc gccggcgatg aactcggccc
     1381 agtcggcgac caccgccggc ttgaaaccgt actccgcgaa atactgctgc atggtttcgc
     1441 ggcggccgag gctctccgct gcgatcaata cgcgctcggg ggtggaggtg acatggcgtt
     1501 tcagcgcgcg cagcggatcg tcggcacggc gatcgacctg gacggcggga agggaatgcg
     1561 tggcgtgtcc gcggcgcgat cccttggcgc gcgcggcgac ggtcccggcc gccggcgctg
     1621 cggcgtccgc atcggtttcc acgaccggcg gctcggtcgc gttctcggcg gatgcggcgt
     1681 attcgccgga ccccgacact ccgtccgagg cgagctcgat gcgggcgaac ggcttgagca
     1741 tgcgatggaa ctcgtcctcc ggcacgaaca gctcgctcgg cgcgagcaac ggccgcgcac
     1801 gatcgcctcg cagcagccgg tagcgcgcgt cggtgtcctc ccagaaacgc cggatcgccg
     1861 agggtatgtc gccatgcagg cagacgatag cctcggccgg cagatactcg gtcagcgtcg
     1921 cggtcgaatc gaaaaacagc ggcaggtaat actcgatacc ggccggcatg atgccgttcg
     1981 acacatcctt gtacagcggt gacttcgacg gatcgccttc gaatacctcg cgaaaccgcc
     2041 cgcgaaagcg cgtgcggccg gcgtcatcct gcggaaactc gcgcgccgga agcagccgta
     2101 cctgcggcac cggatagacg gtgcgctggg tgtccacatc gaaagtgcgg atcgattcga
     2161 tctggttgtc gaacaggtcg atgcggtagg ggagggggct gcccatcgga aacagatcga
     2221 tcaagccgcc gcgtatgccg aattcaccgg gagaaaccac ttgcgtgaca tgctgatagc
     2281 cggccagcgc gagctgcgag cgcaggcgat cgacatcgag cgtctcgcct tgctcgatga
     2341 aaaacgtgaa cgccgcgaga tacgacggcg gcgcgaatcg gtacagcgct gtcgtcgcgg
     2401 cgacaatcac cacatcacaa tcgccgcgcg acatacggta gagcgtcgcg aggcgctccg
     2461 acaccagatc ctgatgcgga gaaaaatgat cgtaaggcag cgtttcccag tcaggcagca
     2521 cggcgacctt gagtgccggc gcgaaccatg gcatttcgac ggccagccgt tgtgctgcca
     2581 gcgcgtcggc gcagacgacg gccaacagct ggcggcgctg tgcggcgtcg gtggcagcct
     2641 gcgcaagggc cagcgcgtcg gccgaaccgg cgaatggctg agcgatggcc ttccgcccgg
     2701 gaggcggcgc ggcgggcaga aggcggggaa gcggaacgaa cggcggcggg ctagacgcca
     2761 gcgacgggga caacggaatc gacaatgcag atgatccggg gaaaaaggtc aattatacga
     2821 gggtcgcatg gcgccgctcc ggacgctgat gcgcccggtc ggcgataatg cggtgatgcg
     2881 acgatttaac gttctcgtcg tccagcccaa cgactatgtc aactacgcac cgtttctcgg
     2941 cgtggcaaag tcgatgtact ttgcgcttcg acggctgggg tacgacgtgc gactggccgg
     3001 caacgaattc ctgcgcgatg cgaccaacgt gatcttcggc gcacatcacc tcgcaggcga
     3061 aatcgccgcg gatctgccgt ccgacaccat cgtttacaac accgaacagc tgcgtgcgga
     3121 cggtgcgctg gtggaagcgc tgactccatt cgtctcgcgc ttcgcgacct gggaaccgga
     3181 ctcgggcaac gtcgaggcgt ggcgcgcgct cgggctgggc agccgcgtcc actacgtcca
     3241 gccgggatac ctgcccgaga gctcgacagt cgccgcggag acgtcgaccg acatcgacgt
     3301 attgttcttc ggcaaggtca acccgcgccg catggtcatc ctcgactcga tcatgcgtgc
     3361 gggaatcagc ctgcacgtcg ccgacggcgt ctatctggca gaccgcgacg cgctggtcgc
     3421 gcgcgccaag atcctgctca atgtgcacgc ggccgacgac tccgcgctcg aaatggcgcg
     3481 agtcgcctac gcgctggcca atcggcgcgc gttggtcacc gagctggggg gcggagcgcg
     3541 ccttgatccg gacctgcgcg acggcgttct cgccggaacg aatgcggaac tagcggcaat
     3601 gtgtcggaca ttgctcgccg acgataaacg gcggctcgag ctcgccgagc ggggattcca
     3661 ggcgttttca cggcgcgacc tggtcgcgac ggtgagaaat gccctcgcgc aggccagcgc
     3721 tggctgaacg gcctgacgcg cgcgttgtcg cccgtgccgt tccggacggc gctagcgcgc
     3781 acgtagaagc gcgcgcatcg gtcctgcggg acgcgcgttc cagaatcctg cgtgtgcgac
     3841 gtcgccaccg acggcagaga gcgacatcgt ttgcaggaca cgcgcgacga tcgccttgaa
     3901 ctccatttgt gcaaagcgga tgcccaggca catgcgggca ccgccggcga aggtgagcag
     3961 cacaaaaggc cgagtgcggc tcgcgctgtc gccgcatttg tagcgatcga tatcgaagcg
     4021 caacggatca gggtagttat cgggtagcag gtgggtcgcc gcgaccgcaa cggcgatccg
     4081 tgttccggga ctgatgcagt aacccgagaa ttcaatcggc gacgccgcga cgcgcggaag
     4141 gttgacgagc ggaggatata gtcgcccgac ctcgagcatg aagcgatcga gctgcggcaa
     4201 tcgatcgaga tcgtcggcgc caagcggctg tgcccgcgtc gatgacgcgc catcgagctc
     4261 cgcggcgagt gtcgcggcga gccgcggcat ctgcgcaagg acgtaaaggg cgcgcgagta
     4321 aagcgaggcg ccggtctcat ggccggcgat gagcaggatc gtcaggtgcg actggatctc
     4381 cgcatcgttg cctgcgagcc cgggctctgc gagcagaacg tcgagcaatg acgccggcgc
     4441 agcaggcctg cgacgtcgtc gttcgcggat ggcggtgcgc agagtgtcgg cgaggatcgc
     4501 gcgtgcggca gcagcgcgct cgaaataggc gtcccttgat tcgcgcgaat aatcctggcc
     4561 atcgagtatc gtgcagatgg cgtgatacgc cggtccgatc gctgcttcgg gcaagccgcc
     4621 tatcgtcgcg gccacggcgc ggaacgcgaa cgcgcgcatc ggagggtaga ggtcgagctc
     4681 gtccccgtcc cgccacgcga gtatgcagtc ctcgacgatc cgtgccatcg ccggctcgta
     4741 ggagcgcagt atcgcgcccg cgaaagcggg cgaccaggtc cggcgttgct gggcgtggag
     4801 tggatcgtcg gtgttgagga ccgccagtcc gcaaccgaca ccgagcacgt ggcgccagcc
     4861 ctgttcgctg ctgaactctc cgcgcctgga aagaagtgcg gtttcggctg cggccgatcc
     4921 gaccagggtg atgcacgggc cggcgtccgg accgaaagga acgttgctgc gcagcacggg
     4981 gccatgcgct gcgcccgctg ccgcctgcag gtgcgggacc gccccggtca gcgagtcgtt
     5041 ggcagacagc accaacgccg gcgccgccag cgacgcgtct gggagccggc tcaccccagc
     5101 gtcatctgac gcgggccggc gctcgccgaa ggcgtccgat cccgcacgcc atccagcgcg
     5161 taagccaagg catcgacgac gcgccgctga tcgtcggcgg tcagccgggc aaacgtcggc
     5221 agcgtgatga tgc
//
LOCUS       DATANS010000212         1714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_725783, whole genome shotgun sequence.
ACCESSION   DATANS010000212 DATANS010000000
VERSION     DATANS010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725783"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1714
                     /locus_tag="VHT22_03030"
     CDS             <1..>1714
                     /locus_tag="VHT22_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3136556.1"
                     /translation="HLPIARQYQILGVLLLLFGILAALMLWLNYRANSQAGASSTTAT
                     EMQMLSQRLARGTALAVQGNAGAFEKIKDSRDRFRADLDALTKGGVVHGTNVDIATNE
                     ALQKALGALDARWERVEKNATTVVDNQAALLSLSKGLDSINQGNNALLELANQASAQI
                     NSAGGSIREVDYTSQLAMLSQRIAKNANSLVSSDEIDPEVAFLLGKDTGTFRDILNGL
                     LKGSDALRLPGAVRAEDARATLAEMQKRFAGYETGVNAILQNMQRLVLAKQAARSINQ
                     DSDPLLDETTALTNEFDGQTTVRSFTLWAAMAFAVLALVVLIAYGWVSREDGRVRAFE
                     SEQENKRNQEAILRLLNEMGNLADGDLTVQASVTEDVTGAIADSINFTIEELRTLVRG
                     INSATDQVAKATTDAQAISNRLYEASQRQNKEIQQASASVLQMAQSINEVSQTAAQSA
                     RVAQQSLAAAEKGGEAVHNQISGMNEIRTQIQDTAKRIKRLGESSLEIGEIVELISDI
                     TEQTNVLALNAAIQAASAGEAGRGFTVVAEEVQRLAERSGEATKQIEAIVKTIQADTQ
                     DAVAA"
BASE COUNT          352 a          537 c          571 g          254 t
ORIGIN      
        1 catctgccga tcgccaggca gtaccagatc ctcggcgtgc tgctcctgct gttcggcatt
       61 ctcgccgcgc tgatgctgtg gctcaactat cgggcgaact cgcaggcggg cgcaagctcg
      121 acgaccgcta ccgaaatgca gatgctgtcg cagcgcctgg cgcgcggaac cgcgcttgcg
      181 gtgcagggca atgccggcgc gttcgagaaa atcaaggact cgcgcgaccg atttcgcgcc
      241 gacctcgatg cgctgaccaa gggcggcgtc gtacacggca caaatgtcga catcgctacc
      301 aacgaagcgc tgcagaaggc gctgggcgcg ctcgacgcgc gctgggagcg ggtcgaaaag
      361 aacgccacaa cggtggtcga caaccaggcc gcgctgctgt cgctgtcgaa ggggctggat
      421 agcattaacc agggtaataa tgcgctgctc gagctggcga accaggcgtc ggcgcagatc
      481 aactcggccg gcggatcgat ccgtgaggtc gattacacct cccaactcgc catgctgtcg
      541 cagcggatcg ccaagaacgc gaattcgctg gtctcttctg acgaaatcga tccggaggtc
      601 gcgttcctgc tgggcaagga caccggcacc ttccgcgaca tcctcaacgg gctgctgaag
      661 ggaagcgacg cgcttcgact gccgggtgcc gtgcgcgccg aggacgcgcg cgcgacgctg
      721 gccgagatgc aaaagcggtt cgccggctac gagaccggcg tcaacgccat cctgcaaaat
      781 atgcagcggc tggtgctagc caagcaagcc gcgcgcagca tcaaccagga ctccgacccg
      841 ttgcttgacg aaaccaccgc gctgaccaac gaattcgatg gccagacgac ggtccgcagc
      901 ttcactttgt gggccgcgat ggccttcgcc gtgctcgccc ttgtcgtcct gatcgcctat
      961 ggctgggtgt cgcgcgaaga cggtcgcgtt cgcgcgttcg aaagcgaaca ggagaacaag
     1021 cgcaaccagg aagcgattct gcggctgctg aacgagatgg gcaatctcgc cgacggcgac
     1081 ctgaccgtgc aagcgtcggt gaccgaggat gtgaccggcg cgatcgcgga ttcgatcaac
     1141 ttcacgatcg aggagttgcg gacgctggtg cgcgggatca actcggcgac cgaccaggtg
     1201 gccaaggcga cgaccgatgc gcaggcgatc tcgaaccggc tgtacgaggc ctcgcaacgg
     1261 cagaacaagg agatccagca ggcgtccgca tcggtcctgc agatggcaca gtcgatcaac
     1321 gaggtgtcgc agaccgctgc gcagtcggcc cgcgtcgcgc agcagtcgct tgccgccgcg
     1381 gaaaaaggcg gcgaagcggt gcacaaccag atatcgggca tgaacgaaat ccgcacgcag
     1441 atccaggaca ccgccaagcg gatcaagcgc ctcggcgaat cgtcgctgga gatcggcgag
     1501 atcgtcgagc tgatttcgga cattaccgag cagaccaacg tgctggcgct gaacgccgcg
     1561 atccaggccg cgtcggcggg tgaagccggc cggggcttca cggtggtcgc cgaggaagtg
     1621 cagcgactgg ccgagcgctc gggcgaagcg accaagcaga tcgaggcgat cgtcaagacc
     1681 attcaggccg acacgcagga cgcggtggcg gcca
//
LOCUS       DATANS010000213         4288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_389534, whole genome shotgun sequence.
ACCESSION   DATANS010000213 DATANS010000000
VERSION     DATANS010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389534"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1649)
                     /locus_tag="VHT22_03035"
     CDS             complement(<1..1649)
                     /locus_tag="VHT22_03035"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEX3136557.1"
                     /translation="MRLDSHDYLPIDASSSLGASASGASFATSTGDMLEIGSFGSGIF
                     RLRVGPNTRPDYGLIAARAQRCDVAQPSRGLWTFTAGDGSLELAGTPLTLRLLHAGKP
                     VLASITDRTRDGQTRLPAIGRARQGQSWLAAYALASGEPVYGLGAQTAMLNKRGQLVH
                     SQVEPAGASPSASGTSAPFCWSPGLGGGAWGVYVHTPSRVLHGVGYPDWSQRSYGLVV
                     DDEALDLFLIAGRDPAQVIERYTAVIGRAPEVPRWSLGAWIARGDDLSADGAVAAASD
                     LRARRVPCDVLMLDGTGPWDPWTSFDFRWDPRGYPAPAAAFAKIRQQGFKVCVCEQPS
                     ISIHSPLFHDLAARKYLMADADGRPYERAAGAEPDDARPGARPAPPSGIIDLTNPAAL
                     AWWRNAHEALFKAGVDVIKTDGGDDVPANAIAFNGDTGQRLSNIYPLLYGRCVFDAAE
                     KFAPPEQAPPLIWSSSGSSGSQRYPVQSSGETQSDWEGLAASIRASLSSGMSGVPYHA
                     CKVGGSYGGEPASAELYLRWLQAGVFASHLELRAGAGRDPWA"
     gene            complement(1709..2584)
                     /locus_tag="VHT22_03040"
     CDS             complement(1709..2584)
                     /locus_tag="VHT22_03040"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018916948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX3136558.1"
                     /translation="MESPFECVLDARASLGECPVWSPDEQALYWVDINGPSLNRFDPK
                     SRVNVAWPMPSSIGSFALREHGGFVAALRDGIWLIDRTGRLGDRMAEAPYDPSHHRFN
                     DGRADRSGRFWVGTMNERRDAASGALYCLDGYFALTRVLDGIMISNGLAWSPDGRTLY
                     HADTPTRTVNAYAFDEVAGTVSGKREFARFDGETERPDGAAVDSDGCYWIAFYRGGKV
                     VRLSPRGERLAEYALPAMCPTMCAFGGPEMRTLYVTTARQNREPDELARLPQSGGLFA
                     MQVDTPGLPEPKFAG"
     gene            complement(2649..3446)
                     /locus_tag="VHT22_03045"
     CDS             complement(2649..3446)
                     /locus_tag="VHT22_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016854682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HEX3136559.1"
                     /translation="MDLPINQFKRAIASGRTPLGAWLGSGAASTAEALGCVGFDFLVV
                     DMEHTPIDTPQMIDILRAIAGTPAQAIVRPPWNDMVMIKRALDAGAQSLLLPFVQNAD
                     EARRAVSYTRYPPEGVRGVAGTHRGSRYGTVSNYLKRASEEICVIVQIETLPALGKLD
                     EIAAVPGIDSIFIGPSDLSASMGHLGDITNSAVQEHLAAAAKACRRAGKPSGIIGPNP
                     EMVARFIDYGYSWVAIGSDMGFMVGRAQELLSRFRNAGTAADKPVAR"
     gene            3573..>4288
                     /locus_tag="VHT22_03050"
     CDS             3573..>4288
                     /locus_tag="VHT22_03050"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX3136560.1"
                     /translation="MRTHFSRSAFIEGARDMLPMTVGLIPFGIVCGVGAIAAGASPVA
                     ALAMSMIVFSGAAQIIAAQLLAAGAPFAVIVLSCFVISLRFLMYSAALAPYLKPVDHR
                     WRNLVAFLLTDQAFASSIRRFRESSDIRANVSYFVGGGSVLWLAWQIATIAGILAGAV
                     IPASWQLEFVVPLCFLALLAPLLHDRTALIVFAIAAVAAIALNSMPMRLSMICAGLAG
                     IVAGVVADRVLGRGTTTEVEP"
BASE COUNT          687 a         1471 c         1412 g          718 t
ORIGIN      
        1 gcccacgggt cgcgcccagc gccggcgcga agctcgaggt gcgaggcgaa gacgccggcc
       61 tgcaaccagc gcagataaag ctccgcggag gccggctcgc cgccgtagct gccgccgact
      121 ttgcaggcgt gatacggaac gccgctcatg ccgctggaca ggctggcacg gatgctcgcc
      181 gccaggccct cccaatcgct ttgcgtctcg ccgctcgatt gcaccggata gcgctggctg
      241 ccgctcgagc cggagctgct ccagatcagc ggcggcgcct gctcgggcgg cgcgaacttt
      301 tcggcggcat cgaaaacgca acggccgtag agcagcggat agatgttgga cagacgctgg
      361 cccgtatcgc cattgaatgc gatcgcattg gccgggacgt cgtcgccgcc gtcggtcttg
      421 atcacgtcga cgccggcctt gaacaaagcc tcgtgtgcgt ttcgccacca cgcgagggcg
      481 gccgggttgg tcaaatcgat gatgcccgac ggaggcgccg gtcgcgcgcc gggacgcgcg
      541 tcatcgggct ccgcaccggc cgcgcgttcg tatggccgcc cgtcggcgtc ggccatcaga
      601 tattttctgg ccgcgaggtc atggaacagc ggcgagtgaa tcgatatcga cggctgctcg
      661 cagacgcaga ccttgaaccc ctgctgcctg attttggcga acgcggcagc gggggccgga
      721 tagcctctcg gatcccatcg gaaatcgaag gacgtccatg gatcccaggg tccggtgccg
      781 tcgagcatca gaacgtcgca gggcacacgg cgagcgcgca gatccgacgc cgctgcgacg
      841 gcgccgtcgg cgctcaagtc gtcgccgcgc gcgatccacg cgccgagact ccagcgcggc
      901 acttcgggtg cgcgtccgat caccgcggtg tagcgctcga tcacctgcgc gggatctcgg
      961 ccggcgatca ggaacaagtc gagcgcttcg tcgtcgacga ccaagccata cgaacgctgc
     1021 gaccagtcgg gatagccgac gccatgcaac acgcgcgacg gcgtgtgaac atagacgccc
     1081 caggctccgc cgccgaggcc gggactccag cagaagggcg cactcgtgcc cgacgcggaa
     1141 ggcgaggcgc cggccggttc gacctgcgaa tgcaccagct ggccacgctt gttcagcatc
     1201 gctgtctgcg caccgaggcc gtagaccggc tcacccgacg ccagcgcgta cgccgccagc
     1261 cacgactgcc cctgtcgcgc gcggccgatc gcgggtaacc gcgtctggcc gtcccttgtc
     1321 cggtcggtga tcgaagcgag caccggtttg cccgcgtgca acaggcgcag cgtcaacggt
     1381 gttccagcca gctcgagcga tccgtcgcct gcggtgaacg tccacaggcc gcgcgatggc
     1441 tgcgcgacat cgcagcgctg tgcgcgcgcg gcgatcaacc cgtaatcggg ccgcgtgttc
     1501 ggaccgacgc gaagacggaa tattccggag ccgaagctgc cgatctcgag catgtcgccg
     1561 gtcgaggtgg cgaacgatgc acccgacgcc gacgcgccga ggctgctcga tgcatcgatc
     1621 ggaagatagt cgtggctgtc gaggcgcatg atgtccggtc aggagtcggc aatgtcgaag
     1681 atcgatgctt ccgcttcagc gacggcgatc agccggcgaa tttcggctcc ggcaaacccg
     1741 gggtatcgac ttgcatcgcg aacagcccgc ccgactgcgg cagtcgcgcg agctcatccg
     1801 gctcgcgatt ctgacgcgcc gtcgtgacgt agagggtgcg catctccggg ccgccgaatg
     1861 cgcacatggt cggacacatc gccggcagcg cgtattcggc gaggcgctcg cctcgcggcg
     1921 acagtcgcac gaccttgccg ccgcgataga acgcaatcca gtaacagccg tcgctgtcga
     1981 ccgccgcgcc gtcgggccgt tcggtctccc cgtcgaaacg cgcgaattcg cgcttgccgc
     2041 tgacggtgcc cgcgacttcg tcgaatgcat acgcattgac cgtgcgcgtc ggcgtatcgg
     2101 cgtgatagag cgtgcgccca tcgggactcc aggcgagccc gttcgatatc atgatgccgt
     2161 cgagcacacg ggtcagtgcg aaatagccgt cgaggcagta cagcgcgccg ctggccgcgt
     2221 cgcgtcgctc gttcatcgtg cccacccaaa accgtccgct gcgatcggcg cgcccgtcgt
     2281 tgaagcgatg gtgcgatgga tcatagggcg cctcggccat gcggtcaccg agcctgcctg
     2341 tccgatcgat caaccagatg ccgtcgcgca gcgccgcgac gaatccgccg tgctcgcgca
     2401 gcgcgaagga gccgatcgac gaaggcatcg gccaggcgac gtttacgcgg gacttcggat
     2461 cgaagcgatt gagcgacggc ccattgatgt cgacccagta gagtgcctgc tcgtcaggcg
     2521 accacaccgg gcattcgccg aggctcgccc gggcatcgag cacgcactcg aacggcgatt
     2581 ccatctcgcg atcccgctgg cttaaaacgt gcatcgtaca agaaaacaag cgcgcccggc
     2641 gctaagcgtc agcgagcaac cggcttatcg gccgcggtac ctgcattgcg gaacctcgac
     2701 agcaattcct gcgcgcgccc caccatgaag cccatgtcgg agcctatcgc gacccatgag
     2761 tatccgtagt cgatgaagcg cgccaccatc tcggggttcg gcccgatgat gcccgacggc
     2821 ttgccggcac gccggcatgc cttggcggca gcggccagat gctcctgcac cgccgaattc
     2881 gtgatgtccc caaggtggcc catcgacgcc gaaagatcgc tcggcccgat gaaaatcgaa
     2941 tcgatgccgg gaacggcggc gatctcgtcc agcttgccca gcgcgggcag tgtctcgatc
     3001 tgcacgatca cgcagatctc ctcgctcgcg cgcttgaggt agttggacac ggtgccgtaa
     3061 cggctgccgc gatgcgttcc ggcgacgccg cgtacgccct ccggcggata gcgcgtatag
     3121 gaaaccgcgc gccgggcctc gtcggcgttc tgcacgaacg gcagcaacag cgattgcgcg
     3181 ccggcatcga gcgcgcgctt gatcatcacc atatcgttcc acggcgggcg cacgatcgcc
     3241 tgcgcgggcg ttccagcgat cgctcgcagg atgtcgatca tctgcggcgt gtcgatcggc
     3301 gtgtgctcca tgtcgacgac gaggaagtcg aacccaacgc agccgagcgc ttccgcggtc
     3361 gatgccgcgc cgcttccgag ccacgcgccg agcggcgtgc gcccggatgc gatcgcgcgc
     3421 ttgaactgat tgatcggaag atccacgaat gactcctcgt tcgggttggc gagcggccat
     3481 tatcgcgcgg ccgagctata atcggcgcga atgctcttaa gggcttgcgg gccggagcga
     3541 tcgtcggccg caggccctta gctttttccc cgatgcgcac ccatttctcc cgttcggcgt
     3601 tcatcgaagg cgcgcgcgac atgctgccga tgaccgtcgg actgatcccg ttcggcatcg
     3661 tgtgcggcgt cggcgcgatc gccgcgggcg cctcgccggt tgccgccctc gcgatgtcga
     3721 tgatcgtctt cagcggcgcc gcccagatca tcgccgcaca gttgctcgct gccggcgcac
     3781 cgtttgccgt gatcgtgctt tcgtgctttg tcatcagcct ccgcttcctg atgtacagcg
     3841 cggcgctcgc gccttatctc aagccggtcg accatcgctg gcgcaacctg gtcgcgttcc
     3901 tgctgaccga ccaggcgttc gcgtcgtcga tacggcgctt tcgcgagtcc tcggacatcc
     3961 gcgccaatgt ctcctatttc gtcggcggcg gctccgtgct ctggctggcc tggcagatcg
     4021 cgacgatcgc cgggatcctg gcgggcgccg tcatcccggc gtcgtggcaa ctggagttcg
     4081 tcgttcccct gtgctttctc gccctgctcg cgccgctgct gcacgaccgc acggcgctga
     4141 tcgtgttcgc gatcgcggcc gtcgcggcga tcgcgctgaa ctcgatgccg atgcgcctgt
     4201 cgatgatttg cgccggcttg gcgggcatcg tcgccggggt cgtcgccgac cgggttctcg
     4261 ggcgcggcac gacgacggag gtcgagcc
//
LOCUS       DATANS010000214         2156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_515507, whole genome shotgun sequence.
ACCESSION   DATANS010000214 DATANS010000000
VERSION     DATANS010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515507"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="VHT22_03055"
     CDS             complement(<1..1443)
                     /locus_tag="VHT22_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX3136561.1"
                     /translation="MNVFYEEEGTFKVGAILADNDTSLQVEAAHGKRSKIKASAVLLR
                     FDQPALAAFADAAQRVAADVDVDFLWECCGADEFSFEVLGKEYFGHAPSALESAALLY
                     KLHGAPMYFYKKGRGRYKAAPAEALKAALASVEKKRLQAEQKQRWTDELLAGRLPPEW
                     PPLVSKLLYAPDKNTLEWKALEAASEQARLSPARVMAKSGALASPHDYHLNRFLFEYF
                     PQGTAAAEVPPVALPADLPHAAVRAFSIDDETTTEIDDAFSVTRLANGNTEVGIHIAV
                     PALGIAPDSPIEAIARERLSTVYFPGQKITMLPEPAIDAFTLAAGGERCALSLYAEVG
                     ADGAVVATRSRCEMVPIVANLRIGALEETFNPDALAAGPIAHEFGAELKFLWEFAQHL
                     QSARGKADVEGEIQRAEYNFYVEGERVRIVERKRGSPIDKVVSELMIYANAEWGGQLA
                     RAGYVAIYRAQSGGVARMTTVASRHDGLGVA"
     gene            complement(1505..2071)
                     /locus_tag="VHT22_03060"
     CDS             complement(1505..2071)
                     /locus_tag="VHT22_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HEX3136562.1"
                     /translation="MIIYIHGFNSSPASFKAKFLHDRFAAFRRAHEFVAPALPPSPAA
                     AAQILETLAREHPEAALVGSSLGGFYATWLAERFNLRAVLVNPALRPYDLLGGEVGRR
                     TNFHTGEEYNFTLEHVAELRELEVDAIDPRRYLLMVETGDEVLDYRHAVERYRGAQQL
                     VIKGGDHGFSDFADYLDQVMEFCAAPAG"
BASE COUNT          368 a          708 c          704 g          376 t
ORIGIN      
        1 cgcgacgccg agcccgtcgt ggcgcgaggc gaccgtcgtc atacgggcga ccccgccgct
       61 ttgcgcgcgg tagatcgcca catacccagc gcgcgcgagc tggccccccc attcggcgtt
      121 cgcataaatc atgagctcgg aaacgacctt gtcgatcggc gagccgcgct tgcgctccac
      181 tatgcgcaca cgctcgccct cgacgtaaaa gttgtattcg gcgcgctgaa tctcgccctc
      241 cacgtcggct ttgccgcgcg cggactgcag gtgctgcgcg aactcccaca ggaatttgag
      301 ctctgcgccg aactcgtgcg caatcgggcc cgccgcgagg gcgtcggggt tgaaggtttc
      361 ttccagcgcg ccgatgcgca gattggcgac gatcggcacc atttcacagc ggctgcgcgt
      421 cgccaccacc gccccgtccg cgcccacctc ggcatacaac gacagcgcgc agcgctcgcc
      481 ccccgccgcg agggtaaacg cgtcgatcgc aggctcgggc agcatggtga ttttctggcc
      541 gggaaagtag accgtcgaca gccgttcccg cgcaatcgcc tcgatcggcg aatccggcgc
      601 aatgccgagc gccggcaccg cgatatggat gccgacctcg gtgttgccgt tcgccaggcg
      661 cgtcacggaa aacgcgtcgt cgatctcggt cgtggtctcg tcgtcgatgc tgaacgcgcg
      721 cacggcggca tgtggcaggt cggcgggcag cgccacgggc ggcacttcag cggcggcggt
      781 gccctgcgga aagtactcga acagaaaacg gttcaaatga taatcatgcg gcgaggccag
      841 cgcgccgctc ttggccatca cgcgcgccgg cgagagcctc gcctgctcgg acgccgcttc
      901 aagcgctttc cattcgagcg tatttttgtc cggtgcatac agcagcttcg acaccagcgg
      961 cggccactcc ggcggcaacc ggcccgccag aagctcgtcg gtccagcgct gtttctgctc
     1021 ggcctgcagg cgcttttttt cgacgctcgc gagcgcggct ttcagcgcct cggcgggcgc
     1081 cgccttgtag cggcccctgc ccttcttgta aaaatacatc ggcgcgccgt gcagcttgta
     1141 gagcagcgca gcggactcga gcgcgctcgg tgcgtgcccg aaatattcct tgccgagaac
     1201 ctcgaacgaa aactcgtcgg cgccgcagca ctcccacagg aaatcgacgt cgacatcggc
     1261 ggcaacgcgt tgcgcggcat cggcaaacgc ggcgagcgcc ggctggtcga atcgcagcag
     1321 caccgcggac gccttgatct tgctgcgctt gccatgcgca gcctcgacct gcagcgacgt
     1381 atcgttgtcg gccaggatgg cgccgacttt gaaagtgcct tcttcttcgt aaaagacgtt
     1441 catgcgggaa acggggcgga gccgcgtggg aacgcgggtt taggcgaggc gcggattata
     1501 aggcttatcc cgcgggggcg gcgcaaaact ccatgacttg atcgaggtag tcagcgaaat
     1561 cgctgaatcc gtggtcgccg cctttgatca cgagctgctg cgcgccgcgg tagcgttcga
     1621 cggcatgccg gtaatcgagc acctcgtcgc cggtttccac catcagcagg tagcgacgtg
     1681 ggtcgattgc atcgacttcg agttcgcgca gctcggcgac atgctcgagc gtgaaattgt
     1741 attcttcgcc ggtgtgaaaa ttcgtccggc gcccgacctc gcccccgagc aggtcgtatg
     1801 ggcgcagcgc ggggttgacc agcaccgcgc gcaaattaaa tcgctcggcg agccacgtcg
     1861 catagaagcc gccgagcgag ctgccgacga gcgccgcctc cgggtgctcg cgcgctagcg
     1921 tttcgagtat ttgtgcggcg gcggctggtg atggcggcag cgccggcgcg acgaattcgt
     1981 gcgcgcgccg gaatgccgcg aagcgatcgt gcaggaattt cgccttgaat gatgcgggcg
     2041 agctgttgaa gccgtgtata tagatgatca cggcgattta gacggcgagg cgccgcgcaa
     2101 gttcgagcag cagcgtgtcg ttgccgcgcg ccgcaaccag cgacagcccc agcgga
//
LOCUS       DATANS010000215         1798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_180271, whole genome shotgun sequence.
ACCESSION   DATANS010000215 DATANS010000000
VERSION     DATANS010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180271"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1784)
                     /gene="kdpA"
                     /locus_tag="VHT22_03065"
     CDS             complement(<1..1784)
                     /gene="kdpA"
                     /locus_tag="VHT22_03065"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEX3136563.1"
                     /translation="MNALGVVQILLFIAVLVALVKPLGTFMAKVYDGERTFLSPVLEP
                     IERGIYRLAAIKPSVESNWKRYALALLLFNFIGLVVVYLLQRLQGVLPLNPQGFAAVS
                     PDSSFNTAVSFASNTNWQGYGGEATMSYLTQMLGLAVQNFVSAASGMAVLVAVIRGFA
                     RRRASEIGNFYVDLTRSTLYILLPLSLLLAVILMSQGVVQSFAAYGSVDLVEPVTIAT
                     PVKDAAGAPVLDAAGNAVTKREQVTQQTIPLGPAASQIAIKQLGTNGGGFFNVNSAHP
                     FENPTPLANFLEVLAILLVPAALCYTFGRMIGDPRQGWAVLIAMLVIFVPLTFGAYVA
                     EQSGNPRLEALVDQNASATQSGGNMEGKEVRFGIANSALWASATTAASNGSVNAMHDS
                     FTPLGGLVPMWLMQLGEVVFGGIGSGLYGMLMYAVVAVFIGGLMVGRTPEYLGKKIES
                     YEVKMASIVILVPPLIVLVGTAIASVTPVAVSGVANPGVHGFSEILYAFSSAGNNNGS
                     AFGGLSANTPFYNIALGIAMWASRYWLMIPVLALAGSLAAKKATAVTAGTMPTHTPLF
                     ITLLVGVVLMVGALTFVPALALGPIVEHL"
BASE COUNT          355 a          585 c          569 g          289 t
ORIGIN      
        1 agatgctcga cgatcggccc gagcgcgagc gcaggcacga aagtcagcgc accgaccatc
       61 agcacaacgc cgaccagcag ggtgatgaac agtggcgtgt gggtgggcat cgtgcccgcg
      121 gtgaccgcag tcgctttctt cgcagccaag ctgccggcga gcgccagtac cggaatcatc
      181 agccagtagc gcgacgccca catcgcgatg ccgagtgcga tgttgtagaa cggcgtgttc
      241 gctgacagtc cgccgaacgc actgccattg ttgttaccag cggacgagaa cgcgtaaagg
      301 atctccgaga atccatgcac gccgggattg gctacgccgc tcacggctac cggcgtcacg
      361 ctggcgatcg ccgtgccgac cagcactatc agcggcggca ccaggatcac gatcgaagcc
      421 atcttcactt cgtaggattc gattttcttg ccgaggtatt ccggcgtgcg tcccaccatc
      481 aggccgccga tgaacaccgc gactacggcg tacatgagca tgccgtacag gccgctgccg
      541 atgccgccga acacgacttc gccgagttgc atgagccaca tgggtacgag cccgccgagc
      601 ggcgtgaacg agtcatgcat cgcgttgacc gagccattgg aagcggcggt cgtcgcactg
      661 gcccacagcg cggagttggc gatgccgaag cgcacttcct tgccttccat gttgccgccc
      721 gactgcgtgg cgctggcatt ctggtcgacc agcgcctcga gccgcgggtt gcccgactgc
      781 tcggcgacat aggcgccgaa cgtcagcggc acgaagatca ccagcatcgc aatcaggacc
      841 gcccagccct ggcgcgggtc gccgatcatg cggccgaacg tgtagcacag cgcggcgggg
      901 acgagcagga tcgccagcac ttccagaaaa ttcgccagcg gcgtcgggtt ctcgaacgga
      961 tgggcggaat tcacattgaa gaatccgccg ccgttggtgc cgagctgctt gatcgcaatc
     1021 tgactcgctg cgggtccgag cggtatcgtc tgctgcgtaa cctgctcccg tttcgtaacc
     1081 gcattgcccg cggcgtcgag caccggcgca ccggcagcgt ccttcaccgg cgtcgcgatc
     1141 gtgaccggct cgaccaggtc gacgctgccg taggccgcga agctctgcac gacaccttgc
     1201 gacatgagaa tcacggcgag cagcagcgac aacggcaaca ggatgtacag agtgctgcgc
     1261 gtgaggtcga cgtagaagtt gccgatctcg ctggcacgcc ggcgcgcaaa accgcggatc
     1321 acggcgacca ggactgccat cccggacgcc gccgagacga agttctggac cgcgagcccg
     1381 agcatttgcg tgagatagct catcgtcgcc tcgccgccgt aaccctgcca gttggtgttg
     1441 ctggcgaagc tcacggcagt gttgaaggac gagtcggggc tcaccgcggc aaagccctgc
     1501 gggttcagcg gcagcacgcc ctgcaaccgc tgcaacaggt acaccacgac gaggccgatg
     1561 aaattgaaca gcagcaatgc gagcgcgtag cgcttccagt tcgattcgac cgacggcttg
     1621 atggcggcga ggcgatagat gccgcgctcg atcggttcga gcaccggcga caggaaggta
     1681 cgctcgccgt cgtacacctt ggccatgaac gtgccgagcg gcttgaccag cgccaccagc
     1741 acggcgatga acaacaggat ctgcaccacg cccagcgcgt tcattcgaat ttctccgg
//
LOCUS       DATANS010000216         1757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_389611, whole genome shotgun sequence.
ACCESSION   DATANS010000216 DATANS010000000
VERSION     DATANS010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389611"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1757
                     /locus_tag="VHT22_03070"
     CDS             <1..>1757
                     /locus_tag="VHT22_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX3136564.1"
                     /translation="WADAYLARTRRLGALDIAQAGDAIAAQAALIGAASPTILIVGFI
                     ELTPQQTRLFTALEGAGASLRQLDSLAVRVGQVARATAPTPREEIAAALSWARAIAID
                     RPAARIGVVVESLAERRDVVLALAEDILDPASILPGHARSGAPFELSLGVRLTQVPLV
                     GAALDLITLSDGPLTLGAAASLLRSPYLPDADALWQRHAALERRWLEQGVRTVSLTDA
                     IAAVAPYAAELATRWTNGRDALRTARATTPREWTDLWRSWLAAAGWPGSRALDSAEFQ
                     ARDAWEKLLVQFASLGAVAPRLGARAAIAALHAMANETLFQPEGSNAPVQILGMLEGA
                     GLDFDALWVAGLSADRWPAAPRPNPLLPIAWQRERGVPRASAQRELEYARVLTERFAR
                     AADEVVFSSATGVDDPPLSPSALVLAHPPSPPLAVPETWSASMTRSAPLESLADESAP
                     AMPPGSAIRGGARAIQTQSDCPFQAVARHRLGAQAWPTAGSGLAPFERGQLAHATLAA
                     FWSSIRDWAMLLAIEADDLDIAIAAAVEKGMACLREARWRSVPDAVRAAESRRLNRLL
                     RAWLDIERGRPPFAVAAVE"
BASE COUNT          240 a          610 c          627 g          280 t
ORIGIN      
        1 cctgggccga tgcgtatctg gcacgaacgc gccggctcgg cgcgctggac atcgcgcagg
       61 cgggcgatgc cattgcggcg caagccgcgc tcatcggggc agcatctccg acgatcctga
      121 tcgtaggatt catcgagctc acgccacagc agacgcggct gttcacggca ctcgaaggcg
      181 cgggcgcgag cttgcggcag ctcgacagcc tagcagtgcg agtcgggcag gtcgcgcgtg
      241 cgaccgcgcc cacgccgcgc gaagaaatcg ccgcggccct ttcctgggcg cgcgccatcg
      301 cgatcgaccg tccggcagcg cgcatcggcg tcgtcgtcga aagtctcgcc gagcgccgcg
      361 acgtcgtgct cgcattggcg gaggacatcc tcgacccggc gtcgattctg cccggtcatg
      421 cacgaagcgg cgcaccgttc gagttgtctc tcggcgtgcg cctgacgcaa gtgccgctgg
      481 ttggtgcagc gctcgatctg atcacgctaa gcgatgggcc attgacgctg ggcgccgcgg
      541 cgtcgctgct gcgctctcct tatctgccgg acgccgacgc gctgtggcag cgtcacgcag
      601 cgctcgaacg tcgctggctc gagcagggcg tgcgcaccgt atcgctgacc gatgcgatcg
      661 cggcggtcgc accgtatgcg gccgagctcg cgacccggtg gaccaacggc cgtgatgcgt
      721 tgcgcaccgc tcgcgcgacg acgccgcggg aatggaccga cctctggcgc agctggcttg
      781 ccgcggctgg ctggcccggt tcgcgtgcac tcgacagcgc ggaattccag gcgcgcgacg
      841 cgtgggagaa gctgctcgtg cagtttgcgt cgctcggtgc cgtcgcgccg cgtctcggcg
      901 cgcgggcggc gatcgcggcg ctgcacgcta tggcgaacga aaccctgttt cagccggagg
      961 gcagcaatgc accggtccag attctgggca tgctggaagg cgccgggctc gacttcgatg
     1021 cactttgggt ggccggcctc tccgccgacc gatggcctgc ggcgccgcgt cccaatccct
     1081 tgttgccgat tgcgtggcag cgcgagcgcg gcgtgcctcg tgccagcgcg cagcgcgagc
     1141 tcgagtacgc ccgcgtgctt accgaacgat tcgcgcgggc cgcggacgag gtcgtgttca
     1201 gctcggcgac cggtgtcgac gatccaccgc tatcgccgtc ggccctggtg cttgcccatc
     1261 cgccgtcgcc gccgctggcc gtgcctgaaa cctggtctgc ctcgatgacg cggagcgcac
     1321 ctctggaaag cctcgccgac gaaagcgcgc cggcgatgcc gccgggatcg gcaatccgcg
     1381 gcggtgcgcg ggccatccag acgcaaagcg attgtccctt ccaggcggtc gcgcggcacc
     1441 gcctcggcgc gcaagcatgg cccacggctg ggtccggcct ggcacccttt gaacgcgggc
     1501 agctcgcgca cgcgacgctg gccgcattct ggagctcgat ccgcgattgg gcgatgctcc
     1561 tggccatcga agccgatgat ctcgacatcg cgattgcggc ggcagtcgaa aagggcatgg
     1621 cctgcctgcg cgaagcgcgg tggcgcagcg tgcccgacgc ggtgcgagcg gccgagtcgc
     1681 gccgcctgaa ccgtctgttg cgggcatggt tggatatcga gcgtggcagg ccgccattcg
     1741 cggttgccgc cgtcgag
//
LOCUS       DATANS010000217         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_180273, whole genome shotgun sequence.
ACCESSION   DATANS010000217 DATANS010000000
VERSION     DATANS010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180273"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1680)
                     /locus_tag="VHT22_03075"
     CDS             complement(<1..1680)
                     /locus_tag="VHT22_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEX3136565.1"
                     /translation="MSKETPDQDIREASLEYHRAHPPGKISILPTKQLINQRDLALAY
                     TPGVAAACDEIVRDPAEARNLTARGNLIGVITNGTAVLGLGAIGPLAGKPVMEGKAVL
                     FKKFAGIDCFDIEIDERDPDRLVDIICALEPTFGGINLEDIKAPECFYVERKCRERMR
                     IPVFHDDQHGTAIIVGAAVLNGLRVVGKEMRAVKLVCSGAGAAALACLDLLVELGVQR
                     QNIWVSDIAGVVYRGRKEEMDDNKARYAQATDARKLADIIPGADIFIGLSAPRVLKPE
                     MLKQMADRPLIMALANPEPEIMPDVAKAARPDAIIATGRSDFPNQVNNVLCFPFIFRG
                     ALDVGATTINEAMKLACVRAIADLAMVEQSDIVATAYGVQDVKFGPDYLIPKPFDPRL
                     IVMIAPAVARAAMASGVATRPIADFDAYHARLSQVVYHSGLLMKPIFQAAKKAPKRVV
                     YAEGEEERVLRAVQVVVDEGLAKPMLIGRPAVIERRLARYGLRIKPGVDVEIVNPESD
                     PRYRAYWTEYYRLAQRKGVSQQFAKIEMRRRLTLIGAMMIHMGDADGMLCGT"
     gene            1812..>2485
                     /gene="ccmI"
                     /locus_tag="VHT22_03080"
     CDS             1812..>2485
                     /gene="ccmI"
                     /locus_tag="VHT22_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEX3136566.1"
                     /translation="MLIFWFLAGALLVAALGVLLWPLLRTQRGGAEPDADSAAIAVFR
                     DQKRAAEQDFASGAITGAEREVVLSDLAQRVADEIGAPPRSPARSTTTRRSWPLALGL
                     LVSIPVLAIFLYGRLGDPAASAVAAATDGGQELSDPQIAAMVGALERRLEQHPDNAEG
                     WALLARSYYVLGRFPAAAEAYTKALARAPDNPDLLADYADTLGMLQSRHLAGKPLVLV
                     ERALAI"
BASE COUNT          393 a          817 c          827 g          448 t
ORIGIN      
        1 cgtcccgcac agcatgccgt ccgcgtcgcc catgtgtatc atcatcgcgc cgatcagcgt
       61 cagcctgcgg cgcatctcga tcttggcgaa ctgctgcgac acgcctttgc gctgcgccag
      121 ccggtagtac tcggtccagt aggcccggta gcgcggatcg gattccggat tgacgatctc
      181 gacgtcgacg ccgggcttga tgcgcaaccc gtagcgcgcc aggcggcgct cgatcaccgc
      241 ggggcgaccg atcagcatcg gcttggccag gccctcgtcg acgacgacct gcacggcgcg
      301 cagcacgcgc tcctcctcgc cttcggcgta gacgacgcgc ttcggcgcct tcttcgcggc
      361 ctggaaaatc ggcttcatca agaggcccga gtgatagacg acctgcgaca ggcgcgcgtg
      421 gtaggcgtcg aagtccgcga tcggacgcgt cgcgacgccg gacgccatcg ccgcccgcgc
      481 caccgccggc gcgatcatca cgatcaatct ggggtcgaat ggcttgggga tcaggtagtc
      541 gggcccgaac ttgacatcct gcacgccgta cgcggtggcg acgatgtccg attgctcgac
      601 catcgcaagg tcggcaatcg cgcgcacgca cgcgagcttc atcgcttcgt tgatcgtcgt
      661 cgcgccaaca tccagtgcac cgcggaaaat gaacggaaag caaagaacgt tgttgacctg
      721 gttcgggaag tcggaccggc cggtggcgat gatcgcgtcg ggccgcgccg ccttggcgac
      781 atcgggcatg atctcgggct cgggattggc cagggccatg atcaacggtc tgtccgccat
      841 ctgcttcagc atttcgggct tcagcacgcg cggcgccgac agaccgatga agatgtcggc
      901 gccggggatg atgtcggcca gctttcgcgc gtcggtagct tgcgcgtagc gcgccttgtt
      961 gtcgtccatc tcctccttgc ggccgcgata aacgacgccg gcaatgtcgg agacccagat
     1021 gttttggcgc tggacaccaa gctccacaag caggtcgaga caggcgagcg cggccgcgcc
     1081 cgcgcccgag cacacgagct tgaccgcgcg catctccttg ccgacgacgc gaagaccgtt
     1141 gagcaccgca gcgcccacga tgatcgccgt gccatgctgg tcgtcgtgga ataccggaat
     1201 gcgcatgcgc tcgcggcatt tgcgctccac atagaagcat tcgggcgcct tgatgtcttc
     1261 cagattgatg ccgccgaacg tcggctcgag cgcgcagatg atatcgacca ggcggtccgg
     1321 atcgcgttcg tcgatttcga tgtcgaaaca gtcgatgccg gcgaatttct tgaacaggac
     1381 cgccttcccc tccatcaccg gtttgccggc gagcgggccg attgcgccga ggccaagcac
     1441 cgcggtgccg ttggtgatga cgcctatcag attgccgcgt gcggtgaggt tgcgagcttc
     1501 ggctggatcg cggacgattt cgtcgcaggc ggcggcgacg cccggcgtat aagcgagcgc
     1561 gagatcgcgc tgattgatca gctgcttggt cggcaggatc gagatcttgc ccggcggatg
     1621 cgcgcgatga tattcgagcg aggcctcgcg tatgtcctgg tcgggcgtct ctttgctcat
     1681 cgttcgtccg cggcaagatg atagcagaag cccgtgccgg cgccttcctg ccggacgacg
     1741 caaagccggc gggcggcgct cgcgctgacc gtataatgcg cgtatcgcgt ctgccgcgga
     1801 attctctgct gatgctcatt ttctggtttc tcgctggggc gctgctcgtt gccgcgttgg
     1861 gcgtgctcct ctggccgctg ctgcgcaccc agcgcggtgg cgcggagccg gacgccgact
     1921 cggccgcaat cgcggtattc agggatcaaa agcgcgccgc cgagcaggac ttcgcatcgg
     1981 gtgcgatcac cggcgccgag cgcgaggtcg tgctgtccga tcttgcgcag cgcgttgccg
     2041 acgaaatcgg tgcgccgccc cgctcgcccg cgcgcagcac tacgacacgt cggtcctggc
     2101 cgctggcgct agggctgctg gtcagcattc cggtgctggc gatcttcctc tacggccgtc
     2161 taggcgatcc cgcagcctcc gccgtcgccg cggccaccga cggaggacag gaattgagcg
     2221 acccgcagat cgccgcgatg gtcggcgcgc tggagcgtcg gctcgagcag catccggaca
     2281 atgccgaagg ctgggcgctg ctggcgcgct cgtattacgt gcttggtcgc tttcccgcgg
     2341 cggcggaagc ctacacgaag gcgctggcgc gggcgccaga caaccccgat ctgctcgccg
     2401 attacgccga tacgttgggg atgctgcaga gtcgacatct cgccggcaag ccgctcgtgc
     2461 tggtagagcg cgcactggcc atcga
//
LOCUS       DATANS010000218         1716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_180283, whole genome shotgun sequence.
ACCESSION   DATANS010000218 DATANS010000000
VERSION     DATANS010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180283"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /gene="pmbA"
                     /locus_tag="VHT22_03085"
     CDS             complement(<1..1340)
                     /gene="pmbA"
                     /locus_tag="VHT22_03085"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEX3136567.1"
                     /translation="MSNAVTSSQLSYSQQDLQDFVSLALAEARGLGASEAEVGVSVET
                     GLSVTARLREVETLEYQRDRGMGVTVYRGKRKGSASTGDLSASAIRDTVAKAFSIAGF
                     TAQDEAAGLPEPDTLAREFPDLDLFHPWSIDPDAARDQANACEAAALDADPRIGNSEG
                     ATLSTHRGMRVFGNSLGFMGNTATTLHSLSCVVVAEAGEEMQRDSWYSTVRDWRDLED
                     GVSIGKQAAARALRRLNARKLSTVTAPVLFVPELARGLFGSFLGAIRGGSQYRRASFL
                     LDAAGEQVFPDWMQISERPHLRKALSSAAFDSEGVATRDREIVERGVLTGYVLSTYSA
                     RKLGLKTTGNAGGVHNLIVSSGSDDFEGLLLRMGRGLVVTELMGQGVNGVTGDYSRGA
                     AGFWVENGAIAYPVHEITIAGNLREMFRNVVAVGSDVDTRGGIRTGSVLLDDMT"
     gene            1381..>1716
                     /gene="yjgA"
                     /locus_tag="VHT22_03090"
     CDS             1381..>1716
                     /gene="yjgA"
                     /locus_tag="VHT22_03090"
                     /inference="COORDINATES: protein motif:HMM:NF016626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HEX3136568.1"
                     /translation="MEERPSKSERKRRSDDLQSLGEALIDLPDSEFNALPLPEQLREA
                     VQLARKITAHGGLYRQKQYIGKLMRKIDAEPIRAAMDAKRERERVEALRFRRIEQWRD
                     RLLQEGPSAI"
BASE COUNT          313 a          579 c          546 g          278 t
ORIGIN      
        1 atcgtcatgt cgtcgagcag caccgagccg gtgcggatgc caccgcgcgt atccacatcg
       61 ctgccgacgg ccacgacgtt gcggaacatc tcgcgcaggt tcccggcaat cgtgatctcg
      121 tggaccggat acgcgatcgc gccgttctcc acccagaagc cggctgcgcc gcgtgaatag
      181 tcgccggtga ccccgttgac gccctgcccc atcagctccg tcaccaccag gccgcgtccc
      241 atccgaagca gcaaaccctc gaaatcgtcg gagccgctgc tcacgatgag gttgtgcacg
      301 ccccctgcgt tgccggttgt cttgagtccc agtttgcgag ccgagtacgt gctgagcacg
      361 tagccggtca gcacgccacg ctcgacgatc tcgcgatcgc gcgttgcgac gccttcgctg
      421 tcgaatgccg cgctgctgag cgccttgcgc aagtgcgggc gctcggagat ctgcatccag
      481 tcgggaaaga cttgctcgcc cgccgcgtcg agcaggaacg acgcgcgacg atactggctg
      541 cctccgcgta tcgcccccag gaacgagccg aacagaccac gcgccaactc gggcacgaac
      601 aaaaccggcg cggtgaccgt cgacagcttg cgtgcattga gccgccgcaa ggcgcgcgct
      661 gcggcctgtt tgccgatgct cacgccatct tccagatcgc gccagtcgcg aacggtgctg
      721 taccaagagt cacgctgcat ctcttcgccg gcttcggcga cgacgacgca gctcagactg
      781 tgcaacgtcg tcgccgtgtt gcccatgaag ccgagcgaat tgccgaacac gcgcatgccg
      841 cgatgagtcg acagcgtcgc gccttcggag ttgccgatgc gcggatcggc atcgagcgcg
      901 gccgcctcgc acgcgttcgc ctgatcgcgc gccgcatccg gatcgatcga ccagggatgg
      961 aacagatcga gatcggggaa ctcacgcgcc agcgtgtcgg gctcgggcaa gccggcggct
     1021 tcgtcttgcg cggtgaagcc agcgatgctg aaggccttcg ccacagtgtc gcggatcgcg
     1081 ctggcactca gatcgccggt gctggccgag cccttgcgct tgcccctata gacagtgacg
     1141 cccatacctc gatcgcgctg atactcgagc gtctcgactt cgcgcaggcg cgctgtcacc
     1201 gataggccgg tctcgacgct gacgccgact tcggcctcgc tggcgccgag cccgcgcgcc
     1261 tccgcgagcg cgaggctcac gaagtcttgc aagtcctgct gggagtagct gagctgagaa
     1321 gaagtgacgg cattcgacat gacggcgatt gtataggctg tagactgtag gctgtagcct
     1381 atggaagaac gccctagcaa aagcgaacgc aagcgccgca gcgacgatct acagtcgctc
     1441 ggcgaggcgc tcatcgacct gccggacagc gagttcaatg cgctgccgct gccggagcaa
     1501 ttgcgcgagg ccgtgcagct cgcgcgcaag atcacagcgc acggcgggct ctatcgccag
     1561 aaacaataca tcggcaagct gatgcgcaag atcgacgccg agccgatccg cgctgcaatg
     1621 gatgcgaagc gcgagcgtga gcgagtcgaa gcgctgcgct tccggcgcat cgagcagtgg
     1681 cgcgaccgcc tgctgcaaga aggtccgagc gcgatc
//
LOCUS       DATANS010000219         3232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_558732, whole genome shotgun sequence.
ACCESSION   DATANS010000219 DATANS010000000
VERSION     DATANS010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558732"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..414
                     /gene="rnpB"
                     /locus_tag="VHT22_03095"
     ncRNA           44..414
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VHT22_03095"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(488..2098)
                     /locus_tag="VHT22_03100"
     CDS             complement(488..2098)
                     /locus_tag="VHT22_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3136569.1"
                     /translation="MKDRYDAVIIGGGHNGLVCACYLAGAGLKVAVFERRGIVGGAAV
                     TEEFHPGFRNSTASYTVSLLHPKVIHELCLKEHGLKIVERPMSNFLPLPGGEYLKVGG
                     ASIDGRGGTQAEVARFSHKDAAALPAYYAMLERVADVLRALLLETPPNVGGGVHELFQ
                     AWKVGGKLKRLPMAHRRDILDLFTKSAGDILDRWFESDPIKAALGFDAVVGNFASPYT
                     PGSAYVLLHHVFGEVNGKRGQWGHARGGMGSISVAMAREALGRGVEIFTDEAVARVLT
                     KDGNAVGIVLADGSEIAAPRVVANVNPKLLYLQLIDAAALDGDFRQRIENYRCVSATF
                     RINVALDAVPDFACLPGDGEHLRSGIIIAPSLAYMERAYFDARQHGWSREPIVELLIP
                     SLVDRTLAPPGKHVASLFCQHANPTLPDGRSWDAVREEVADLMIATVNRHAPNFAAAV
                     IGRRVLSPLDLEREFGLIGGDIFHGALSLDQLFSARPVLGNAHYRGPLNGLYMCGAGT
                     HPGGGVTGVPGHNCAREILRDLRRRRHG"
     gene            complement(2217..3005)
                     /locus_tag="VHT22_03105"
     CDS             complement(2217..3005)
                     /locus_tag="VHT22_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3136570.1"
                     /translation="MGFLAGKKILITGLLSNRSIAYGIAHACKREGASLAFTYVNDDL
                     KDRVVKLAADFGGAPVLPCDVTHDDQIAALFSAVEREWGGLDGLLHSIAFAPREALAG
                     DFLNAISRQAFAIAHDVSSYSFAALAKGARPLMQGRQGALLTLTYLGAVRAMPNYNVM
                     GLAKASLEANVRYLAQCLGPEGVRVNGISAGPIKTLAAAGIGGFSGILHFVAKTAPLR
                     RNVTIEEVGNVAAFLLSDLASAVTGEITYVDCGFSTIVPGMGEE"
BASE COUNT          586 a         1090 c         1052 g          504 t
ORIGIN      
        1 gtggcggaaa atcacctctc ccgccgtgct accattcgcg tccgaagcca gccagaccgt
       61 cgctgcccgc cggtgagaac cagtgcgggg agaggaaagt ccgggctccg cagagcagga
      121 tgctgggtaa cgcccagacg ctataagtcg ttcgcgagag cggcgatgaa ggcgaggaac
      181 agggccacag agacgagtcg cccccggttc gggtcaaacc gtgccgggag cgggtgaaac
      241 gcggcaacct ccatccggag caacaccaag taagcggacg acgacgctgc tcgccgagtc
      301 cgcgggtagg tggctcgagc gccgcggtaa cgcggcgcct agacgaatga cggccatagg
      361 acgcgccttc ggcgcgtccc gtaacagaac ccggcttatc ggctgacttc gactttattg
      421 cgcagtgcct ctggcgcccc gcggttttcg cggggcgtct ccttttcaca tgtccaatcg
      481 cgtcgattca gccgtgacgc cgccggcgca agtcgcgcag aatttcgcgc gcgcagttgt
      541 gcccgggcac gccggtcacg ccccctccgg gatgggtgcc ggcaccgcac atgtacaaac
      601 cattcagcgg cccgcggtag tgcgcgttac caaggaccgg ccgcgccgaa aacagttggt
      661 cgagcgagag cgcgccgtgg aagatgtcgc cgccgatcag gccgaactcg cgctcgagat
      721 cgagcggcga cagtacacgg cggccgatca ccgcggcggc gaagttcggc gcgtggcgat
      781 tcaccgtcgc gatcatcagg tcggcgacct cttccctcac ggcgtcccac gagcggccgt
      841 caggcagcgt cggattggcg tgctggcaga agaggcttgc aacgtgcttg cccggcggcg
      901 cgagcgtgcg atcgaccagc gaaggaatca gcagctcgac gatgggttct cgcgaccagc
      961 cgtgctggcg cgcatcgaaa taggcgcgct ccatgtaagc gagcgatggc gcgatgatga
     1021 tgccggagcg caggtgttcg ccgtcgccgg gcaggcaggc gaagtcggga acggcgtcaa
     1081 gcgcgacgtt gatgcgaaac gtcgcggaga cgcagcggta attctcgatc cgctgccgga
     1141 agtcgccgtc gagcgccgca gcatcgatca gctggaggta gagcagtttc ggattgacat
     1201 tggcgacgac ccggggcgcg gcaatctcgc tgccgtcggc cagcacgatg ccaaccgcgt
     1261 tgccgtcctt ggtcagcaca cgcgcaacgg cctcgtcggt gaagatctcg acgccgcgcc
     1321 cgagagcctc gcgcgccatc gcgacggaga tcgagcccat gccgccgcgc gcgtgccccc
     1381 attgtccgcg cttgccgttc acctcgccga agacgtggtg cagcagcacg tatgcggatc
     1441 ccggcgtgta cgggctcgcg aaattgccga cgaccgcatc gaagcccagc gccgccttga
     1501 tcgggtccga ctcgaaccag cggtcgagaa tgtcgcctgc gctcttggtg aacaggtcga
     1561 ggatgtcgcg gcgatgtgcc atcggcaggc gcttgagctt ccccccgacc ttccacgcct
     1621 ggaacagctc gtgcacgcca cccccgacgt tcggcggcgt ttccaggagc agcgcgcgca
     1681 ggacatcggc gacccgttcg agcatcgcgt agtacgccgg gagcgccgcc gcgtccttgt
     1741 gcgaaaaccg cgccacttcg gcctgcgtcc cgccgcgtcc atcgatgctc gcgccgccga
     1801 ccttcaaata ttcgccgccc ggcaacggca ggaagttcga catcggccgc tcgacgatct
     1861 tcaggccatg ctccttcagg cacaactcgt ggatgacttt cggatgcagc aggctgaccg
     1921 tatagctcgc cgtcgagttg cggaatcccg gatggaattc ctcggtcacc gcggcgccac
     1981 cgacgatccc gcgccgctcg aacaccgcga ctttcaatcc cgcgccggcg agatagcagg
     2041 cacagacgag gccgttatga ccgccgccga tgatcaccgc gtcgtaacga tccttcagac
     2101 gcggcggggc cgcgctcagg acagactcgc gcgcgaagtg cgcgttcacc ggcagcgcgg
     2161 atcagacgcg gcgcaaccaa ccgtcagcgc gaagctggcg cgctcgcgaa tcgagttcac
     2221 tcctcgccca tgccgggaac gatggtcgaa aatccgcagt cgacgtaggt aatctcgccg
     2281 gtgaccgcgc tggcgaggtc ggacagcagg aacgcagcga cgttgcccac ttcctcgatc
     2341 gtgacattgc gccgcagcgg cgcggtcttc gcgacaaagt gcagaatgcc cgaaaagccg
     2401 ccgatgccgg ccgcagcgag cgtcttgatc gggccggccg atatgccgtt gacgcgcaca
     2461 ccttccggac ccaggcattg cgcgaggtag cgcacgttgg cctcgaggct cgccttggcc
     2521 agccccatca cgttgtagtt cggcatcgcc cgcaccgcgc ccagataggt cagcgtcagc
     2581 aacgcgccct gcctgccttg catcagcggc cgcgcgccct tggccagcgc ggcgaagctg
     2641 tacgagctca cgtcgtgcgc gatggcgaaa gcctggcgcg agatcgcatt gagaaagtcc
     2701 ccggccagcg cctcgcgcgg cgcgaacgcg atcgaatgca gcaacccatc caacccgccc
     2761 cattcgcgct cgaccgcact gaaaagcgca gcgatctggt cgtcgtgcgt cacatcgcac
     2821 ggcagcactg gcgcgccgcc aaaatcggcc gccagcttga ccacgcggtc cttcagatcg
     2881 tcgttgacgt aggtgaaggc cagggacgcg ccttcgcgct tgcacgcatg cgcgatgccg
     2941 tacgcgatcg agcgattgga cagcaggccg gtgatcagga ttttcttgcc ggcgagaaat
     3001 cccatggcga tccttgtcga ttgatgcgcc ggcgcggcaa cggccgctca tagacgcaag
     3061 cagggtctag aatttctcga tgacgccgca ggcgagccag tcgttggcca cgccgaattc
     3121 gggtttggga tcgggatcga gcttgccatg aacaacgact gaatgcccga tgacgtcgtt
     3181 cgccgccccg gtggcgaccg acagccccgt cacctgggcc tcgagattcg ct
//
LOCUS       DATANS010000220         2397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_137651, whole genome shotgun sequence.
ACCESSION   DATANS010000220 DATANS010000000
VERSION     DATANS010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137651"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VHT22_03110"
     CDS             complement(<1..516)
                     /locus_tag="VHT22_03110"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEX3136571.1"
                     /translation="MDKVLHRDRWVVLAALAAVVALSWAYLAYIVLTQPVMDMDAIAM
                     AAMPPPVWNAGYFAATLAMWMVMMVGMMLPSAAPTVLLFDALRIHGDSARRRDRRATA
                     LFAASYVLAWAAFSVLATTAQWALTASKLLSAMMTGKSPAFAGSLLMIAGAYQLTPLK
                     TACLSQCRSPAE"
     gene            complement(516..1871)
                     /locus_tag="VHT22_03115"
     CDS             complement(516..1871)
                     /locus_tag="VHT22_03115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008544935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3136572.1"
                     /translation="MSRLPDRADYVIIGAGIHGLSTSMHLAQKLKASGKTVGAGGVRI
                     VVLDKTGIGAGASGIACGVVRNNYYQPAMRELMAQCVEVWESDAEAYSYHPVGYMQIS
                     PEVMRAQVGTIYEQQKAIGYESTFIEGEADSLRYMQGLLHDWQAKNITSVLHEKRGGY
                     ANNQASLAGLERKTRALGVDIVTGVAVTGFRSDGGSGAIRAVVTDAGTIACDQVVVAV
                     GPWINSIWNMLALPKQIAVKGRDGAVHEGVRMWHYMALQEGTLGVDPDFQKTNDGAMP
                     PVIHVDTDAPLYSDRDGRVITDQLWGLYYKPDFHFGGVQGGAMPATVERDADQVRVDP
                     YGPKSPEFVVDDNFADMWTSALAHCQKRFEGKHGVYRNEPSGGIGCFTPDSFPVFDRF
                     LENVYVIADSNHGYKMIGVGKLVTDELLGAKPALLEPFRFSRYAAGKLHPVSRSPFPW
                     S"
     gene            complement(1979..>2397)
                     /locus_tag="VHT22_03120"
     CDS             complement(1979..>2397)
                     /locus_tag="VHT22_03120"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX3136573.1"
                     /translation="DEAQYSLPFPVATALIFGRVGAAEIGAPGLSDPRVARLLATFSL
                     TEDDEFSRRFPAERWARVRIGLADGRSLVSEPAQACGGAEHPLSDEEITAKYREMAEP
                     VLGSRRSSRIAVLAAALPGDATALPALIDELLSPTT"
BASE COUNT          423 a          804 c          765 g          405 t
ORIGIN      
        1 ttcggccgga ctgcggcatt ggctgaggca agcggtcttc aacggcgtca attgataggc
       61 gccggcgatc atcagcaacg acccggcgaa agcgggactc ttgccggtca tcatcgccga
      121 cagcagctta gacgcggtca acgcccattg cgccgtcgtc gcgaggacgc tgaacgcggc
      181 ccaggcaagc acatagctgg cggcgaagag ggccgtcgcg cggcggtcgc gccgccgagc
      241 ggaatcgccg tgtatccgca acgcatcgaa cagaagcacg gttggcgcgg cgctgggcag
      301 catcatgcca accatcatca ccatccacat cgccaaggtc gccgcgaagt agccggcgtt
      361 ccagacgggt ggcggcatcg ccgccatcgc gatcgcatcc atgtccatca ccggctgggt
      421 gagaacgatg taggcaaggt acgcccacga cagggcgacg acagccgcca gcgcggccag
      481 aacgacccaa cgatcgcgat gcagaacttt gtccatcagc tccagggaaa gggactgcgg
      541 ctcaccggat gcagcttgcc cgcggcgtaa cgcgagaacc ggaagggctc gagcaacgcg
      601 ggcttggcgc cgagcaactc gtcggtgacc agctttccga caccgatcat cttatagccg
      661 tgattggagt cagcgatcac gtagacgttc tcaaggaacc ggtcgaatac cggaaagctg
      721 tcgggcgtaa agcagccgat tcccccggac ggctcgttgc ggtatacgcc gtgcttgcct
      781 tcgaaacgct tctggcaatg cgcgagcgcc gaagtccaca tgtcggcgaa attatcgtcg
      841 acgacgaact ccggcgactt cggcccatac ggatcgacgc gcacctggtc ggcgtcgcgc
      901 tcgaccgtag ccggcatcgc accgccctgg acgccgccga agtggaagtc gggcttgtaa
      961 tacaggcccc atagctggtc ggtgatcacg cgtccgtcgc ggtccgagta cagcggcgcg
     1021 tcggtgtcga cgtgtatcac cggcggcatc gcgccgtcgt tggtcttctg gaaatccgga
     1081 tcgactccca gcgtgccttc ctgcagcgcc atgtagtgcc acatgcgcac gccttcgtgc
     1141 accgcgccgt cgcgtccctt gaccgcgatc tgcttcggca gcgcgagcat gttccagatc
     1201 gaattgatcc atgggccgac ggcaacgacg acctggtcgc aggcgatcgt tccggcgtcg
     1261 gttacgaccg cgcggatcgc gccggaaccg ccgtcgctgc gaaaccccgt caccgcgacg
     1321 ccggtcacga tgtcgacgcc cagcgcccgc gtcttgcgct cgagaccggc gagtgacgcc
     1381 tggttgttcg catagccgcc gcgcttttcg tgcagcaccg aggtgatgtt cttcgcctgc
     1441 cagtcgtgaa gcagcccctg catgtagcga agcgaatcgg cttcgccctc gatgaacgtc
     1501 gattcgtagc cgatcgcctt ctgctgctcg tagatcgtcc cgacctgcgc gcgcatgacc
     1561 tcggggctga tctgcatgta gccgaccggg tgatagctgt aggcttccgc gtcgctttcc
     1621 cagacctcga cgcattgcgc catcagctcg cgcatcgccg gctgatagta gttgttgcgc
     1681 acgacaccgc aggcgatgcc cgaagcgccg gcgccgatgc ccgtcttgtc gagcaccacg
     1741 atacgcacgc cgcccgcgcc gacggtcttg ccgctcgcct tgagcttttg cgcgaggtgc
     1801 atcgacgtcg acaggccgtg gatgccggcg ccgataatga cgtagtccgc gcggtcagga
     1861 agtcgagaca ttgcgattgt ccttggttgg ggttgcaatc atcgatcatc tcgacatcgt
     1921 ttgccagcca ccgccccgaa cgcgacgccg tctgacgcga aagcgacgcc gcggtgtttc
     1981 aggtcgtagg ggacaacagc tcgtcgatca gcgccggcaa cgccgtcgcg tcacccggca
     2041 gtgcggccgc cagcacggcg atgcgcgagc tccggcgcga cccgaggacc ggctcggcca
     2101 tctcgcggta cttggctgtg atttcctcgt cagacagcgg atgctccgct ccgccgcacg
     2161 cctgcgccgg ctcggagacg agcgaccggc cgtcggcgag tccgatacgg acccgcgccc
     2221 agcgctcggc cggaaagcgg cgcgagaatt catcatcttc ggtcagggag aacgttgcga
     2281 gcaggcgcgc gactcgtgga tcgctcaagc ccggggcgcc gatctcggcg gcgccgactc
     2341 ttccgaaaat cagcgctgtc gccaccggaa acggcaggct gtattgcgcc tcgtcgg
//
LOCUS       DATANS010000221         3729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_558737, whole genome shotgun sequence.
ACCESSION   DATANS010000221 DATANS010000000
VERSION     DATANS010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558737"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..115
                     /locus_tag="VHT22_03125"
     CDS             <1..115
                     /locus_tag="VHT22_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HEX3136574.1"
                     /translation="RKYLNRLSDLLFVLARVLNRSAEREDVLWRKDRRHAE"
     gene            161..751
                     /locus_tag="VHT22_03130"
     CDS             161..751
                     /locus_tag="VHT22_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005273902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3136575.1"
                     /translation="MAVDPAQQLERSARLMELLARTALADQTAFEQLYRLTSPHLYGV
                     AIRILRESAAAEEVLQESFVNVWHHAGSYVAARSQPLTWLTSIVRNRCLDRLRKHDVD
                     TVSIDDEDHAMTIADRSPLPLDLLLSGAEAGAVRRCVEALDAGQKQAIALAFYQGLSH
                     SELARHLKQPLGTVKSWIRRGLERLKACLDGAGVQR"
     gene            783..1490
                     /locus_tag="VHT22_03135"
     CDS             783..1490
                     /locus_tag="VHT22_03135"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX3136576.1"
                     /translation="MNYRTPERAEALAAQYVLGTMSARARTRFARLSRRDPVLAAAIR
                     AWEDRLSPLAESVAPVEPPASVWSTILARIDRAAAGSTPERRTVWATVSLWRGLALAG
                     FATAVALALVLLSPPLERPAATLVVVLAGQDAKPALVASADRNGRLLTIKAVAPLSPG
                     ADRSLQLWALPATGNPRSLGLIPASGVVQIALPVDAGTALQNIPALAVSLEPRGGSPT
                     GLPTGPVLYSGPVERLY"
     gene            complement(1528..2541)
                     /locus_tag="VHT22_03140"
     CDS             complement(1528..2541)
                     /locus_tag="VHT22_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011394916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX3136577.1"
                     /translation="MKLATLKDGSRDGRLVVVSRDLSRAVAVPQIAPTLQAALDDWDG
                     TQALLADAYRTLNAGEVTGAIPFDPALAHSPLPRAYQWADASAYVNHVELVRKARGAT
                     MPAEFWTDPLMYQGGSDSFIGPRDDIVFADEAFGIDMEAEVAVVTGDVPMGTTPQGAA
                     SSIRLLMLVNDVSLRNLIPGELAKGFGFFQGKPASAFSPVAATPDELGAAWRDGKVHL
                     PLLTFVNDVLFGRPNAGVDMTFDFPTLIAHAARTRELEAGSIVGSGTVSNREDGGPGR
                     PVAQGGAGYACIAEQRTVETLRDGKPSTPFLRFGDRVRIDMHDAAGKSIFGAIDQRVR
                     QAD"
     gene            complement(2579..>3729)
                     /locus_tag="VHT22_03145"
     CDS             complement(2579..>3729)
                     /locus_tag="VHT22_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX3136578.1"
                     /translation="DADTGEDKMEKGARRTGRSAWEIAELYTASFVEDMQRLNLELPT
                     VLCRATDHIGEQIEFIADIEKNGYTYRTSDGVYFDTSKQPDYGYLARLDRAGLDAGHR
                     VELGEKKSATDFALWKFSPPGEKRQMEWPSPWGVGFPGWHIECSAMAQKYLGDYFDIH
                     CGGEDHIPVHHTNEIAQTEARVGTRLANFWMHGYFLLSNDAKMAKSDGEFLRIQGLIE
                     RGYDPLAYRYLCLTAHYRSQLNFTWDALDAAAIGLDRMRRAVHALPTGGEPQRSYLER
                     FTGVVNDDLNLPKALAIAWELLRGDLPDRAVTRATLLAFDRIFGLGLETWRPPVETVP
                     EAVNALAAARAAARAAKQWAEADRLRAELHAAGWEMEDRGDGYLLRRR"
BASE COUNT          602 a         1263 c         1227 g          637 t
ORIGIN      
        1 gcgaaagtat ctgaaccggc tgtccgacct gctgttcgta ctggcgcgtg tgctcaatcg
       61 cagcgccgag cgcgaagatg tgctgtggcg caaggatcgc cgccatgccg aatgatcatt
      121 gcgtcgcggc gcggctgcgc cgcggcacgc gagaccgctg atggccgtcg atcccgcgca
      181 gcagctggag cgcagtgcgc gcctgatgga gctccttgca cgcacggcgc tggccgatca
      241 gacggcattc gaacagctct accgtctgac ctcaccgcat ctgtatgggg tcgcgatccg
      301 tatattgcgt gaatcggcag ccgcggagga ggtgctgcag gagagcttcg tcaacgtatg
      361 gcatcacgcc ggctcctacg tcgcggccag gagccagcct ctgacgtggc tgaccagcat
      421 cgtgcgcaat cgctgcctcg accggctgcg caagcacgat gtcgacacgg taagcatcga
      481 cgatgaggac cacgccatga cgattgccga tcgaagcccg ctgccgctgg atctgctgct
      541 gagcggcgcc gaagccggcg ccgtcaggcg ctgcgtcgag gcgctcgacg ccggacagaa
      601 acaggcgatc gcgctggcgt tctatcaggg cctgtcgcat tcggagcttg cgcggcacct
      661 caagcagccg ctgggcacgg tcaaatcatg gatcaggcgc ggattggagc gcctgaaggc
      721 ctgcctcgac ggcgccggcg tccaacgctg agcgcgagcg agacggacat cggccaaccg
      781 cgatgaacta ccggacaccc gaacgcgccg aggcgctggc tgcgcaatat gtgctgggca
      841 cgatgtcggc acgggctcgc acccgcttcg cccgtctgtc gcgccgcgat cctgtgctgg
      901 ctgccgcgat acgcgcctgg gaggatcgtc tgtcgccgct ggccgaaagc gttgcgcccg
      961 tcgaaccgcc ggcaagcgtc tggtcgacca ttctcgcccg catcgatcgc gccgccgccg
     1021 gatccacgcc cgagcggcgc acggtctggg caaccgtcag cctgtggcgc gggcttgcct
     1081 tggccggatt cgccaccgcg gtcgcattgg cgctggtgct gctgtcgccg ccactcgaac
     1141 gtccggcggc cacactggtc gtcgtccttg ccgggcagga tgcgaagccc gcattggtgg
     1201 cctcggcgga ccgcaacggg cgcttgctga cgatcaaggc ggtggcaccg ctttcgcccg
     1261 gcgccgatcg atcgttgcag ctctgggcgc tgccggcgac gggcaacccg cgctcgctgg
     1321 gcctcatccc ggcgtccggc gtcgtgcaga tcgcgctccc ggtcgacgcg ggcacggcgc
     1381 tgcagaacat ccccgccctc gcagtcagtc tcgagcctcg cggtggatcg cccaccgggc
     1441 tgccgaccgg gcccgtgctc tattccgggc cggtcgaacg actgtattga tcggaccgag
     1501 gatcgcgggt cggcgctgaa gcggcgatca atccgcttgc cgcacccgct ggtcgatcgc
     1561 gccgaagatc gatttcccgg cagcgtcgtg catgtcgatg cgcacgcggt cgccgaaacg
     1621 cagaaacggc gtgctgggct tgccgtcgcg cagcgtttcc accgtgcgct gttcggcgat
     1681 gcaggcgtag cccgcgccgc cttgcgcgac cgggcggcca ggcccaccgt cttccctgtt
     1741 cgataccgtg cccgagccga cgatgctgcc cgcttcgagc tcgcgtgtcc gcgcggcatg
     1801 cgcgatcaag gtcggaaaat cgaacgtcat gtcgacgccc gcgttaggac ggccgaaaag
     1861 cacgtcgttg acgaaagtca gcagcggcag atgaaccttg ccgtcgcgcc aggcggcgcc
     1921 cagctcgtcg ggcgttgccg ccaccggcga gaacgccgac gctggcttgc cctgaaaaaa
     1981 gccgaaaccc ttggccagct ccccgggaat cagattgcgc aggctcacat cgttgaccag
     2041 catcagcaag cgtatcgacg acgccgcccc ttgcggcgtc gtgcccatag gcacgtcgcc
     2101 ggtgaccacc gcgacctcgg cttccatatc gatgccgaat gcttcgtcgg caaagacaat
     2161 atcgtcgcgc gggccgatga agctgtcgga accgccctga tacatcagcg ggtcggtcca
     2221 gaactcggcg ggcatcgttg caccgcgtgc cttgcgcacc agttcgacat ggttgacgta
     2281 cgcagacgca tcggcccact ggtaggcgcg gggcaacggc gaatgcgcga gcgcaggatc
     2341 gaacggtatc gcgccggtga cctcgcccgc gttcagcgtc cggtacgcat cggcaagaag
     2401 cgcttgcgtg ccgtcccagt cgtcgagcgc ggcttgcagc gtaggcgcga tttggggcac
     2461 cgcgaccgcc cgcgaaagat cgcgcgacac gacgaccaga cgtccatcgc ggctgccgtc
     2521 cttgagcgtg gcgagcttca tgcttcgatc ggctcgtggt cagcccgcac gcgcgctact
     2581 acctgcgcct cagcaggtag ccatcgccgc gatcttccat ttcccatccg gccgcatgca
     2641 attcggcgcg caggcgatcc gcttcggccc attgcttggc cgcgcgtgcg gcagcgcggg
     2701 ccgcggccag cgcattgacc gcttcgggca cagtctcgac cggcggccgc caggtctcga
     2761 gcccgaggcc gaagatccga tcgaaggcga gcaaggtcgc ccgcgtcacc gcacgatcgg
     2821 gaagatcgcc gcgcagcagc tcccaagcga tcgccagcgc cttcggaaga ttcaggtcat
     2881 cgttgacgac gccggtgaac cgttcgaggt acgaccgctg cggctcgccg ccggtgggca
     2941 gcgcgtgcac cgcgcggcgc atccggtcaa ggccgatggc cgccgcgtcg agcgcatccc
     3001 aggtgaaatt gagctggctt cgatagtgcg cggtcaggca cagatagcga taggccagcg
     3061 ggtcgtaacc gcgttcgatc aacccctgga tgcgcaggaa ctcgccgtcc gacttcgcca
     3121 tcttcgcgtc gttcgagagc agaaagtagc cgtgcatcca gaaattggcg agccgcgttc
     3181 cgacgcgggc ttcggtctgc gcgatttcgt tggtgtgatg gaccggaata tggtcctcgc
     3241 cgccgcaatg gatgtcgaaa tagtcgccca ggtacttctg cgccatcgcc gagcattcga
     3301 tatgccatcc gggaaagccg acgccccatg ggctcggcca ttccatctgc cgcttctcgc
     3361 cgggcggcga gaatttccac aatgcgaagt cggtcgcgct ctttttttcc ccgagttcga
     3421 cccgatggcc tgcatcgagg cctgctctgt ccagccgcgc cagatagccg taatcaggct
     3481 gcttgctggt gtcgaagtac acgccgtcgg acgtgcggta ggtatagccg ttcttttcga
     3541 tgtcggcgat gaactcgatc tgctcgccga tgtgatcggt cgcgcggcag agcaccgtcg
     3601 gcagctcgag gttgagacgc tgcatgtcct cgacgaacga cgcggtataa agctcggcga
     3661 tttcccacgc actgcgtccg gtgcggcgag cgcccttttc catcttgtct tcgccggtgt
     3721 cggcgtcgg
//
LOCUS       DATANS010000222         2276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_53673, whole genome shotgun sequence.
ACCESSION   DATANS010000222 DATANS010000000
VERSION     DATANS010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53673"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VHT22_03150"
     CDS             complement(<1..345)
                     /locus_tag="VHT22_03150"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEX3136579.1"
                     /translation="MFKRTSASSKAGGAAVGGWSSTARHNIVASLAGAALLAALVVVL
                     SFWAFRQIEDSVAARLQTYTLISNANALLSDITDAETGQRGYSLTGNEAFLKPYLAVR
                     KTIEPNLQELRRL"
     gene            complement(637..1605)
                     /locus_tag="VHT22_03155"
     CDS             complement(637..1605)
                     /locus_tag="VHT22_03155"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX3136580.1"
                     /translation="MSTARALCGLLGVAALQSALAADVYPSRPIRFLVPGATGSSQDV
                     LSRIVANKLAQQMNQQVIVDVRAGASGVIGIELGKAAAPDGYTIVAATSTLFASLPAL
                     KSNLSYDPDRDFAPLSRMAMVANVMTVNAGLGVDTVSDLVKLAKAKPGQLNYGSAGNG
                     SPAHLAGAMFDVLAQVKTNHVPYKGAAQALTDLIGGQLQYLVTSPLVAMPHAKGGRIK
                     VLATTGAKRDPLIPELPTVADTVPGYDITQWWGVVVPARTPPPIAARLHAELVKALQS
                     PDVRDLVAKQGASVQPESPAQFSAFMRAERARVANLGKQANIRLDD"
     gene            complement(1609..>2276)
                     /locus_tag="VHT22_03160"
     CDS             complement(1609..>2276)
                     /locus_tag="VHT22_03160"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX3136581.1"
                     /translation="PDILCLQELQQTSRDFIDQALPGHQRVDDPFRGWTTEGNIYWRD
                     SLFERIEHGGEDFGIVPASDRRLFWVRLKIRGYNRTMLVGTAHLTSQRNKLECETGQS
                     PRVEQTRRIIHALKRLNLADEPLFFMGDMNDPVHPPRMLQEAGYVSCFAALGVQTPPT
                     YLCYPTANVAPGDRVVNQSIDWLVANQHARAVAAAVPHFYLKDAAPSDHWPVHAVYEI
                     APA"
BASE COUNT          398 a          705 c          771 g          402 t
ORIGIN      
        1 aagccggcgc aactcctgca ggttgggttc gatggtcttg cgcaccgcga ggtacggttt
       61 caaaaacgct tcgttacccg tcaatgagta accccgctgc ccggtctccg catccgtgat
      121 gtctgacagc agcgcattgg cgttgctgat caaggtgtag gtttggagcc gggcggcaac
      181 cgaatcctcg atttgcctga aagcccagaa cgacagcacg acgaccagcg cagcgagcag
      241 ggccgcaccg gccagcgagg ccacgatatt gtgacgggcg gtagaggacc agccgcccac
      301 cgctgcacca ccagccttgc ttgaagcgct tgttcgcttg aacatgcggc ctttcaggaa
      361 tgcgttgtat tcctcctaat gcatatcccg caccgcacgg agagtcggcc gagtagtcac
      421 tatcgggtga aagtggtcca gggccggcgg cccgggcgtg caaggatcct ttcgcgctct
      481 cccggcgcgt aaaccgcgct ccggcgctac agccgtgaac ctgcgcagtt aaggcgaaac
      541 cacgcgtaaa tctttgcatt gcgtgcaggg gctgtctgta cgccagctaa catatgacgg
      601 ctagcgtatc gacacagcgc ggcggcgcgc cagcgcctaa tcgtcgaggc ggatattggc
      661 ctgcttgccg agattggcga cccgcgcacg ctccgcgcgc atgaatgccg agaattgcgc
      721 gggtgattcg ggttgcacgc tcgcgccctg ttttgccacc aggtcgcgca cgtccggcga
      781 ctgcagcgct tttacgagct cggcatgcag gcgcgcggcg atcggcgggg gcgtgcgcgc
      841 gggcacgacg acgccccacc attgcgtgat gtcgtagccg ggcacggtat ccgcgacggt
      901 cggcagttcc gggatcagcg gatcgcgctt cgcgccggtc gtcgcgagca ctttgatgcg
      961 gccgcctttg gcgtgcggca tggcaacgag cggggatgtg acgagatatt ggagctggcc
     1021 accgatcagg tcggtcagcg cctgggccgc gcccttatat ggcacgtgat tggttttgac
     1081 ttgcgcgagc acgtcgaaca tggcgccggc gagatgggca ggcgagccgt tgcccgccga
     1141 gccgtaatta agctgacccg gcttggcttt cgcaagcttg acgagatcgg acaccgtgtc
     1201 gacaccgagg cccgcattga ccgtcatcac gttggcgacc atcgccatgc gcgagagcgg
     1261 cgcgaaatcg cggtccggat cgtacgagag gttcgactta agcgcgggca ggctcgcgaa
     1321 caaggtcgat gtcgcagcga cgatcgtata gccgtcgggc gcggcggctt tgccgagctc
     1381 gatgccgatc acgcccgacg ctccggcgcg cacgtcgacg atcacctgct ggttcatttg
     1441 ctgcgcaagc ttgttggcga cgatgcgcga cagcacgtcc tgcgagctgc cggtcgcgcc
     1501 gggcaccaaa aagcggatcg gtcgtgatgg atagacgtcg gccgcgagcg cggactgcag
     1561 cgccgcgacg ccgagcaggc cgcagagcgc gcgcgctgtg ctcacgccct acgccggagc
     1621 aatttcatag acggcgtgga ccggccagtg gtcggaaggc gccgcgtcct tgagataaaa
     1681 atgcggcacc gcggctgcga cggcgcgcgc gtgctggttg gcgaccagcc agtctatgct
     1741 ttgattgacg acgcgatcgc cgggcgccac gttggcggtc ggatagcaca ggtaagtcgg
     1801 cggcgtttgc acgccgagcg cggcaaagca actgacgtag ccggcttcct gcagcatgcg
     1861 cggcggatgc accggatcgt tcatgtcgcc catgaagaaa agcggctcgt cagcgaggtt
     1921 cagccgcttc agcgcatgga tgatgcggcg cgtctgctcg acgcgcggcg actggccggt
     1981 ttcgcattcg agcttgttgc gctgggaggt caggtgcgca gtgccgacca gcatcgtgcg
     2041 gttatacccg cggatcttga gacgtaccca gaatagccgc cggtcgctgg ccggcacgat
     2101 gccgaagtcc tcgccgccgt gctcgatgcg ctcgaacagc gagtcgcgcc agtagatatt
     2161 gccctcggtc gtccagccgc gaaacggatc gtcgacgcgc tggtgcccgg gcagcgcctg
     2221 gtcgatgaaa tcgcgcgacg tctgctgcaa ttcctgcagg cacaggatgt cgggat
//
LOCUS       DATANS010000223         1998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_180505, whole genome shotgun sequence.
ACCESSION   DATANS010000223 DATANS010000000
VERSION     DATANS010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180505"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..1884)
                     /locus_tag="VHT22_03165"
     CDS             complement(262..1884)
                     /locus_tag="VHT22_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3136582.1"
                     /translation="MGARREVVSAVAERYRSAGRREKGRVLDELTAVTGWHRKHAVRA
                     LRAKPPTQAAATVPRKRRYNAAIRDAVTALWEASDRVCGKRLEVMIPALLPALERHGR
                     LTLSRNERALVLSASAATIDRMLVDVKVAAAGGRRRRVGFYSAIRREVPIRTFNDWSD
                     PPPGFCEIDLVAHGGTSVAGSFIQRLTMVDIATGWTECLPLVARDGSLVVEAMTRAQG
                     LFPWLMRGADFDNDSAFMNDVVVPWCRAQRIEVTRSRAYKKNDQAFVEQKNGAVVRRL
                     VGYGRFDGIETARVMARLYAAARLHVNFFQPSFKLKEKRRQGAKVIKRYHAPATPYER
                     ALTHPKLSNAIKRRLRETYRSLDPVALLAEIRRCQDELGARIARRGLAAAADGGPADP
                     LAFARALATNASTGEVRATHRRVKRKYKTRMRMPSKLDPHLATIEHWLKAEPQITALA
                     ILRRLAAIDPDTFCDKQHSIVQRLLKALRRKAAHRVIAETTTEGGEAKLPNAGVGGPA
                     VGLRPPSGPPTPPPLQPHHQRSAPAASGNIPP"
BASE COUNT          338 a          683 c          631 g          346 t
ORIGIN      
        1 atctaacgga aattacctcg gcaacgacgc gcgcgggaga tgcgttaaga cggcgcattc
       61 cagacgcagt gcggccactg cgtcccggtg gagcggtaat tcaccactgc ggcggaaccc
      121 gccgcgaccg tgtcaacatg acaaccaagg gggcagtttt tgatggcgtg aggggagcat
      181 gtgaccttca ggtatcggta tggcgtcatg cgcattcgcc ttgttgtcaa tcggcgtgga
      241 attttgaccc cccgtattgc ctcacggagg aatgttaccg gaggcagccg gggcggatcg
      301 ctgatgatgc ggttggagtg gcgggggtgt tggcgggccg gagggagggc gtagtccgac
      361 cgcaggaccg ccaacacccg cgttgggcaa ttttgcctcg cccccttcgg tagtggtctc
      421 ggcgatcact ctgtgcgccg ccttgcgcct gagcgccttg agcagccgtt gcacgatcga
      481 atgctgcttg tcgcagaagg tatccggatc gatcgcagcc agccggcgca ggatcgcgag
      541 cgcggtgatc tgcggctcgg ccttgagcca gtgctcgata gtagctagat gcgggtccag
      601 cttcgagggc atgcgcatgc gcgtcttgta cttgcgtttc acacgtcggt gcgtcgcgcg
      661 aacctcgccg gtacttgcat tcgtcgcgag cgcgcgtgcg aaggcgagcg ggtctgccgg
      721 gccgccatca gctgccgcag caagcccccg cctagcgatg cgcgcgccca gctcgtcctg
      781 gcaccgacgg atttcggcga gcagcgccac cgggtcgaga ctgcgatagg tctcgcgcag
      841 ccgccgcttg atggcattcg acagcttggg atgggtcagc gcccgctcat agggcgtcgc
      901 cggagcatga tagcgcttga tcacctttgc cccctggcgc cgcttctcct tgagcttgaa
      961 cgacggctgg aagaaattga cgtgcaggcg cgccgccgcg taaaggcgcg ccatcacacg
     1021 agccgtctcg atgccgtcga agcggccgta gccgacgagc cggcgcacga cggctccgtt
     1081 cttctgttcg acgaacgcct ggtcgttctt cttgtaggcg cgcgagcgcg tcacctcgat
     1141 cctctgcgca cggcaccaag gcaccacgac atcattcatg aaggcgctgt cgttatcgaa
     1201 gtcggcaccg cgcatgagcc acgggaacag gccctgcgcc cgcgtcatcg cttcgacaac
     1261 caggctgccg tcgcgcgcca ccagcggcag gcattccgtc cagcccgtcg cgatatccac
     1321 catcgtcagc ctttgaatga acgagcccgc gacgctcgta ccaccgtgag ccacgaggtc
     1381 gatctcgcag aagccgggcg gcggatcgga ccaatcgttg aacgtgcgga tcggcacctc
     1441 gcgccgaata gccgagtaaa agccgacgcg acgccgccgg ccgcccgctg ccgcgacctt
     1501 cacgtcgacc agcatccgat cgatggttgc agcactcgcc gacagcacca gcgcacgctc
     1561 attgcgcgac agcgtcagcc ggccgtgccg ctccagcgcc ggcagcagcg cagggatcat
     1621 cacctcgagc cgcttgccgc acacccgatc ggacgcttcc cacagcgccg taaccgcgtc
     1681 ccggattgcg gcgttgtatc gtcgcttgcg cggcacggtc gcggccgcct gcgtcggcgg
     1741 cttcgctcgc agcgcccgca ccgcatgctt gcgatgccag cccgtcacag ccgtaagctc
     1801 gtcgagaacc cgacccttct cacgccgccc cgccgatcgg tagcgctccg ccaccgccga
     1861 caccacctcg cgccgcgcgc ccatgctgat gttccccacc atgcacgcca gcgatccgat
     1921 tcgcgagcgc caacccagtc atgacaggac ttcttcggta acaatttgga tgaggcaatg
     1981 cgccgtggct ctcgatag
//
LOCUS       DATANS010000224         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_180545, whole genome shotgun sequence.
ACCESSION   DATANS010000224 DATANS010000000
VERSION     DATANS010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180545"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VHT22_03170"
     CDS             <1..232
                     /locus_tag="VHT22_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX3136583.1"
                     /translation="VSPDSPAEQAGVKAGDIILAVGNEAVRSQAEFYRKIWGRGAAGS
                     DIPLRLLQGIDVRDINVHSIDRIDYFRQKTSY"
     gene            343..>2009
                     /locus_tag="VHT22_03175"
     CDS             343..>2009
                     /locus_tag="VHT22_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136584.1"
                     /translation="MPLYAITIFCSAFLLFLVQPIMAKQILPWFGGSANVWTTCLVFF
                     QLALLLGYAYSDLVVRRLHARAQLRLHLALLALSCLMLPIVPGAHWKPLGGESPAPLI
                     LGLLAMTVGLPYFLLSTTSPLVQSWFARRFPGRSPYRLFALSNLASMLALAGYPFALE
                     PWVTTRMQSYGWSAAYLVFVLLCAACAWYSLRTQPPTDVKAPEGSALASEDRPTAARQ
                     VLWVVLAATGSFLLLAVTNHICENISSIPLLWIVPLSIYLLTFILCFDSSRWYRRDLI
                     AATLAAALGVMGWTLASRGLTHRLELQIAVFCIGLFIACMFCHGELARLKPPPRYLTR
                     FYLMVSAGGALGSTLVGIVAPLVLPAYFELSAGLVACALLLALQMRRRHPVFIMLAVA
                     AVLFTLGTAGWGIREFYDGTILATRNFYGVLRVQEGGVSTGRHRSLIHGTILHGNQYL
                     APELARIPTTYYTKNSGVGRALDSMHPTTRLLKVGVIGLGTGTIAAYGSRGDIYRFYD
                     INPAVIRIANREFSYLSDSEATVETPLGDARLNLEREPAQGFDLLAI"
BASE COUNT          297 a          661 c          619 g          432 t
ORIGIN      
        1 cgtctcgccc gacagccctg ccgagcaggc cggcgtcaag gcgggcgaca tcatcctggc
       61 ggtcggcaat gaagccgtgc gctcgcaagc cgagttctat cgcaagatat ggggacgcgg
      121 cgcggcgggc agcgacatcc cgctgaggct gctgcaaggt atcgacgtgc gcgacatcaa
      181 cgtgcattcg atcgaccgga tcgattactt ccgccagaag accagctact gagtcctccg
      241 ccggggctcg cagcacgtta aaggcgacgc acgcagttcc tggcgcgcac gtacaatgcg
      301 cgctgcccgc gcaaggcttt aggcgcgcct cactcccctc cgatgccgct ctacgcgatc
      361 acgattttct gcagcgcgtt cctgctgttt ctcgtgcagc cgatcatggc caagcagatc
      421 ctgccgtggt tcggcgggtc ggccaatgta tggaccacct gtcttgtctt cttccagctc
      481 gccctgctgc tgggctacgc ctattccgat ctggtcgtgc gccggcttca cgcccgcgcc
      541 cagttgcggc tgcacctggc attgctcgcg ctgagttgcc tgatgctgcc gatcgttccc
      601 ggcgcgcatt ggaagcccct gggcggcgag agtccggcgc cgttgatcct cggtctgctc
      661 gctatgacgg tcggattgcc gtatttcctg ttgtcgacga ccagtccgct cgttcagtcg
      721 tggttcgcgc gacgatttcc cggccgcagc ccctatcgcc ttttcgcatt gtcgaatctc
      781 gcgtcgatgc tggcgctcgc cggctatccg ttcgcgctcg agccgtgggt gacgacccga
      841 atgcagtcct acggatggtc cgccgcctac ctggtgttcg tactgctgtg cgccgcatgc
      901 gcgtggtaca gcttgcggac tcaaccgcca accgatgtga aggcgccgga aggttcggca
      961 ctggcgtccg aggacaggcc gacggccgcg cggcaagttc tatgggtggt gctcgccgcg
     1021 accgggtcat tcctgctgct cgccgtgacc aaccatatct gcgagaacat ttcgtcgatc
     1081 ccgctgttgt ggatcgtgcc gttgtcgatc tatctgctga cgttcattct ttgtttcgac
     1141 agctcgcgct ggtaccgccg cgatctgatt gccgcgacgc ttgcggccgc cttgggtgtc
     1201 atggggtgga cactcgccag ccgtgggttg acgcacaggc tcgagcttca gatcgccgtc
     1261 ttctgcatcg ggctattcat cgcctgcatg ttctgccacg gcgagcttgc ccgtctcaag
     1321 ccgccgccgc gttatctgac ccgcttctat ctgatggttt cggccggtgg cgccctcgga
     1381 tcgacgctgg tcggcatcgt cgcaccactg gtcctgcctg cgtacttcga gctctcggcg
     1441 ggcctcgtcg cctgcgccct gctgctcgca ctgcagatgc gccgccggca tccggtgttc
     1501 atcatgcttg ccgtcgcagc tgtgctcttc acgctcggta cagccggctg gggcattcgc
     1561 gagttctacg acggcacgat tttggcgacg cgcaacttct atggcgtgct tcgtgtgcag
     1621 gaaggcggcg tcagcacggg tcgtcatcgc tcgctgatcc acgggacgat tctgcacggc
     1681 aatcagtatc tcgccccgga gctggcgcgt attccgacaa cttattacac gaagaactca
     1741 ggcgtgggcc gagcgctcga ttcgatgcat ccgacgacgc gactgctgaa ggtcggagtc
     1801 atcgggttgg gaacgggcac gatcgcagcc tacggcagcc ggggcgacat ctatcgcttc
     1861 tacgacatca atccggcagt gattcgcatc gccaaccgcg aattcagcta tctcagtgac
     1921 agcgaggcga cggtcgagac gccgctcggc gatgcccggc tcaatctcga gcgtgagccg
     1981 gcgcagggct tcgatctgct ggccatcga
//
LOCUS       DATANS010000225         1646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_473811, whole genome shotgun sequence.
ACCESSION   DATANS010000225 DATANS010000000
VERSION     DATANS010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473811"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VHT22_03180"
     CDS             <1..721
                     /locus_tag="VHT22_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEX3136585.1"
                     /translation="TLDWARRIVGDTRAMRQEMKSLKHSLSGELKIAAIPTVLGMVAQ
                     LTTPFRARYPDVSFRVQSCTSADVLGLLENLEVDAGLTYIENEPIGKVRTIPLFSETY
                     RLLTSPDAMFGDRKQVTWKEVGQVPLCLLTPDMQNRRIIDRALRSVGAEATPSLTSNS
                     LLVLYTHVKTGRWASVMPAKLAETLGLSDSVRSIPIVDPVVDYSVGLVIPQRDPMTPL
                     IAALVQVAREVAPTLEDSAGA"
     gene            complement(718..1644)
                     /locus_tag="VHT22_03185"
     CDS             complement(718..1644)
                     /locus_tag="VHT22_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3136586.1"
                     /translation="MPSSHALFVFDAAARNGSFTAAAAELNVTQPAVSRMLGQFEDYL
                     GVRLFDRTNGRAALTEDGEILYRHVSDGFRSIEGGLIELDRRRKGTETVTLSVSSAFT
                     THWLMPRIDKLQRQFPTVDLRFQLISGPLRGPVDNVDLGMRFRDRYDPARNGALVMKE
                     VMLPICSPAYRDADSADGPIEANTIIRLADTPGDWAAEYPAFLTKRSGPAKSLSFSDY
                     AVVVQAALLGQGIALGWLTVAAHWLLTGALVPASETLSTTRRLCELVHPHDRSIRPVV
                     SEIRDWIIEQMQSDMAAIDRLYPSLGLMPASY"
BASE COUNT          289 a          534 c          549 g          274 t
ORIGIN      
        1 cacgctggac tgggcgcgcc ggatcgtcgg cgacacccgc gcgatgcggc aggagatgaa
       61 gagcctcaag cactcgcttt ccggcgagct gaagatcgcg gcgatcccga ccgtgctcgg
      121 catggtcgcc cagctgacga cgccgtttcg cgcgcgctat ccggacgtga gctttcgtgt
      181 ccagtcctgc acttcggccg acgtgctggg cctgctggaa aatctcgagg tcgatgccgg
      241 gctgacctat atcgagaacg agccgatcgg caaggtgcgc accattccgc tcttcagcga
      301 gacctatcgc ctgctgacgt cgcccgatgc catgttcggc gatcgcaagc aggtgacctg
      361 gaaggaagta ggccaggtgc cgctgtgcct gctgacgccc gacatgcaga accgccgcat
      421 catcgaccgc gcgttgcgct cggtaggcgc ggaggccacg ccgtcgctga cctcgaattc
      481 gctgctggtc ctctacaccc acgtgaagac cgggcgctgg gcaagcgtga tgccggcaaa
      541 actagcggag accctggggt tgtccgactc cgtccgcagc attccgatcg tcgatccagt
      601 cgtcgactac agcgttggac tggtgatccc gcagcgcgac ccgatgacgc cgctgatcgc
      661 ggcgctggtg caggtcgccc gcgaagtggc gccgacgctg gaagatagcg cgggcgccta
      721 atagctcgcg ggcatcagcc ctagagacgg gtagagccgg tcgatggcgg ccatgtcgct
      781 ctgcatctgc tcgatgatcc agtccctgat ctcgctgacg accggccgga tcgatcggtc
      841 atgcggatgc accaattcgc aaagccgccg cgtcgtgctg agcgtttccg aggccgggac
      901 cagcgcgccg gtcagcagcc agtgcgccgc gaccgtgagc cagccgagcg caatgccttg
      961 gccgagcagc gccgcctgca ccaccaccgc gtagtcggaa aagctcaacg acttggcggg
     1021 ccccgatcgt ttggtgagaa aggccggata ttccgcagcc caatcgcccg gcgtatcggc
     1081 aaggcggatg atggtgttgg cctcgatcgg cccgtccgcg ctgtcggcgt cgcgataggc
     1141 cgggctgcag atcggcagca tgacctcctt catcaccagt gcgccgtttc gcgccggatc
     1201 gtatcggtcg cgaaaccgca tgccgagatc gacattgtcg accggcccgc gcagcggacc
     1261 ggaaatcagc tggaagcgga gatcgaccgt cggaaactgg cgctgcagct tgtcgatgcg
     1321 gggcatcagc caatgggtgg tgaatgccga cgagaccgag agcgtgaccg tctcggtacc
     1381 cttgcggcgg cgatcgagct cgatcaggcc gccctcgatg ctgcggaatc catccgatac
     1441 gtggcgatag aggatctcgc catcttccgt aagcgccgcg cggccattgg tccggtcgaa
     1501 cagccggacg ccaagatagt cctcgaactg gccgagcatc cggctgaccg cgggctgggt
     1561 gacgtttagt tcggcggccg ccgcggtaaa actgccgttg cgcgccgcgg cgtcaaagac
     1621 gaacagcgcg tggctcgagg gcagct
//
LOCUS       DATANS010000226         6045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_306076, whole genome shotgun sequence.
ACCESSION   DATANS010000226 DATANS010000000
VERSION     DATANS010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306076"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VHT22_03190"
     CDS             <1..656
                     /locus_tag="VHT22_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX3136587.1"
                     /translation="VAAQTLAAKELEAAAGDIEYRDGVFSIAGTDRRIGLFDLARRQP
                     QHKIVLDSVSSVADATWPNGCHICEVEVDPETGAVAVVAYWSVNDVGRVVNPMIVVGQ
                     LEGGAAQGIGQALCEEFVYDRDSGQALSATLLDYALPRAAMIEHFEMTMDESTPCRNN
                     AMGVKGVGELGTIGATPAVVNAVIDALARAGVGAPAEALQMPLTSERVWRALGGRRV"
     gene            complement(653..1660)
                     /locus_tag="VHT22_03195"
     CDS             complement(653..1660)
                     /locus_tag="VHT22_03195"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX3136588.1"
                     /translation="MRIAVIGAGAIGGMVAVRLALSGQTVTVVDQGAHLDAIRANGLR
                     LLHHDGREEHVQNVSAVASCDEAGTQDLVFLALKAYVLEAVAPQMPALFADDTIVVPL
                     QNGLPWWYFQNFRGPHAGYRLRTADPNGTIERNIEPSRLIGCVVYPAGEIAAPGVIRH
                     LEGDRFPVGELDGSESARVKSLSDTLTAAGLKAPVLADIRSEIWLKLWGNMSFNPISA
                     LTHATLVDICEEPATRELAAEMMREAQAIAAKLGVTFRVPLEKRIEGAAKVGKHKTST
                     LQDIEAGRDVEVDALIGSVIELGTLTATPIPATRAVHAMLKLLVRTMREQRARVVLQP
                     A"
     gene            complement(1753..2520)
                     /locus_tag="VHT22_03200"
     CDS             complement(1753..2520)
                     /locus_tag="VHT22_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX3136589.1"
                     /translation="MPRWARINHNGRECFGTIEGDAVRVHSGDMFAHVTPTGESISLA
                     DAKLLTPVVPGKMVALVDNYRALVAKLGHAVPNEPLYFLKAGSSFLAGGETIRMPKSY
                     SGKVIYEGELGIVIGRRLSQASEADAEAGIFGYTCINDVTAIEIIARDPGFAQWTRAK
                     SFDTFGVFGPVIATGIDPSTLIVKTILNDQERQNYPVADIIFPPAKLASLISHDMTLE
                     AGDIIACGTSVGVGSMKPGSTVSIVIGGIGALTNRFE"
     gene            complement(2549..3922)
                     /locus_tag="VHT22_03205"
     CDS             complement(2549..3922)
                     /locus_tag="VHT22_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX3136590.1"
                     /translation="MANDAHIAADLDAPPITRILADFVAAHPSRGWEDSVDREAHRSL
                     LNWVGCAIGASRHPTVEAALAAVAELEPGAQAMILGRSERVDVASAALLNGITSHTFD
                     FDDTHLKTIIHPAGPVASAVLALAERQRTTGRELVDAVVLGIDVECRIGNAIYPDHYD
                     RGWHITGSTGMLGAAAACARMLKLDTGKTAMALGIAASQPIGVREQFGSMSKAFHIGG
                     AARAGLTSALMARHGYTASMRALEAPRGLLRTYSSKCDWKEISDELGKRFEISFNTYK
                     PFACGIVIHPSIDGCRQLRDAHRLRADDVERIDLLVHPLVLELTGKRAPKSGLEAKFS
                     VYHACAAAMLFGQAGEAEFSDAIARRPEVIALRDRVHAKVDAAIDETAADVTIRCNDG
                     RRLHLKVEHAIGSLQRPMSDSDLERKFHGLVDPILGADRAARLVDVCRTLGDASDVEA
                     LRAAATA"
     gene            complement(3922..4917)
                     /locus_tag="VHT22_03210"
     CDS             complement(3922..4917)
                     /locus_tag="VHT22_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX3136591.1"
                     /translation="MLSSGIPGLIRLFALIAVALPGVTALGQTRPLDYPNRTIKIIAP
                     VQPGGGVDLVARTVADRLGVALGVPIVVDNQSGGGGVVGSLATARAAPDGYTLMIGYV
                     GTHGTNPAVRKLPYDAIKDFTPIAMIAGTPNVLIVPPSIPVSTVQEFVAYVRANPGKL
                     SYGSAGPGTLTHLAMEQLKVAANLDIVHVPYRGIGPAITDILGGQTQALFPGLAAALP
                     HIKAGKMKPLAVTGTRRHPLLPEVPTFDESGYKGFDGVQWYGIVGPANLPGPIVKRLN
                     EEIGKLLQSPDLAERLSGEALQPMPMTSEQFGQYMRDDIDKWSKLAKDRGIQIGE"
     gene            complement(5020..5847)
                     /locus_tag="VHT22_03215"
     CDS             complement(5020..5847)
                     /locus_tag="VHT22_03215"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX3136592.1"
                     /translation="MSESGQTFTDQVDYISGRTRLFGIVGHPIEQVRSPEMFTAEFRR
                     RALDAILVPLHVLPDDFEALLPQLLRLSNLDGVIFTIPYKLRACALADRLGEQARIVG
                     AINALGRRPGGWRGDMFDGIGCVDAFRRRGIPLIGRHALLLGAGGAGSAIGVALASER
                     PASLRVYDVDATRAEALAARIGRAFPGLTVSAGAPQLDGIDILLNATPSGMLDDARLP
                     IAITSLPRAMTVFDAIVKPETTPLLALAERCGCTTVRGREMMRGQIARIVDFFLASD"
BASE COUNT          974 a         1979 c         2030 g         1062 t
ORIGIN      
        1 tcgtcgccgc gcagactctg gcggcgaagg agctcgaagc cgcggccggc gatatcgagt
       61 atcgcgacgg cgtattcagc atcgccggca ccgaccggcg gattggcctc ttcgacctcg
      121 cgcgccggca accgcagcac aagatcgttc tcgactcggt gagctcggta gccgacgcca
      181 cctggcccaa cggctgtcac atctgcgaag tcgaggtcga cccggaaacc ggcgctgtcg
      241 ccgtggtcgc ctactggtcg gtcaacgacg taggccgcgt cgtcaatccg atgatcgtcg
      301 tcgggcagct cgagggcggc gccgcgcagg ggatcggcca ggcgctgtgc gaggaattcg
      361 tgtacgaccg cgactcgggg caggcgcttt cggcgacctt gctcgactat gcgcttccgc
      421 gcgcggcgat gatcgagcat ttcgagatga cgatggacga atcgacgcct tgtcgcaaca
      481 acgcgatggg cgtcaagggc gtcggcgagc tgggcacgat aggggcgacg ccggcggtcg
      541 tcaatgcggt gatcgatgcg ctcgcgcggg ccggtgtcgg cgcgccggcc gaagccttgc
      601 agatgccgct gacgtccgag cgggtctggc gcgcgctcgg cggccgacgc gtctaggccg
      661 gctggaggac gacgcgcgcg cgttgctcgc gcatcgtccg caccagcagc ttcagcatcg
      721 cgtgcacggc gcgcgttgcc ggtatcggcg tcgcggtcag cgtcccgagc tcgatcaccg
      781 atccgatcaa ggcgtcgact tcgacgtcgc gcccggcctc gatgtcctgc agggtcgacg
      841 tcttgtgctt gccgactttc gccgcacctt cgatccgctt ttccagcggt acgcgaaagg
      901 tgacgcccag cttggccgcg atcgcctgcg cttctcgcat catctcggcg gcgagctcgc
      961 gcgtcgcggg ctcctcgcag atatcgacca gcgtcgcgtg ggtgagcgcg ctgatcgggt
     1021 tgaagctcat gttgccccac agcttcagcc agatttcgga acgaatgtcg gccagaaccg
     1081 gcgccttgag tccggccgcg gtcagcgtgt cggacaggct cttgacgcgg gcgctttcgc
     1141 tcccgtccag ctcgccgacg ggaaagcggt cgccctcgag atgccggatc acgccgggcg
     1201 cggcgatttc gccggcggga tagacgacac aaccgatcag gcgggaaggc tcgatgttgc
     1261 gctcgatcgt gccgttcggg tccgccgtcc gcaagcgata gccggcatgt ggcccgcgga
     1321 aattctggaa gtaccaccag ggcaggccgt tctgcagcgg cacgacgatc gtatcgtcgg
     1381 cgaaaagcgc gggcatctgc ggtgcgacgg cttcgagcac ataggctttg agcgccagga
     1441 ataccaggtc ctgcgtgccg gcttcgtcac agctcgccac cgcgctcacg ttttggacgt
     1501 gctcttcccg gccgtcgtga tggagcagcc gcaacccgtt cgcgcgaatc gcatccagat
     1561 gcgcaccctg gtcgacgacc gttaccgtct gtccggatag cgcgagccgc acggctacca
     1621 tgccgccgat ggcccctgcg ccgattaccg caattcgcat gattcctccc gttgggcgcc
     1681 gctcgtgccc gaatgtgcgc gccgatgcgg gtgtatcccg cggcgtgcgc gtcgcttgtc
     1741 ggcggcgccg ctctattcga aacgattggt cagcgcgccg atgccgccga tgacgatgct
     1801 caccgtactt ccgggcttca tcgagccgac gccgaccgac gtgccgcagg cgatgatgtc
     1861 gcccgcctcc agcgtcatgt cgtgcgaaat gaggctcgcc agcttcgctg gtggaaagat
     1921 gatatcggcg accggatagt tctgcctctc ctggtcgttg aggatggtct tgacgatcag
     1981 cgtcgacgga tcgatgccgg tcgcgatcac cgggccgaat acgccgaagg tgtcaaagct
     2041 cttggcgcgc gtccactgcg caaagcccgg gtccctggcg atgatctcga tcgcggtgac
     2101 gtcgttgatg caggtgtagc cgaagatgcc ggcctccgcg tcggcttcgc tcgcctggga
     2161 aagccgccgg ccgatgacga tccccagttc cccttcgtag atgaccttgc cgctgtacga
     2221 cttcggcatg cgtattgtct cgccgccggc gagaaacgag ctgccggcct tcaggaaata
     2281 gagcggctcg ttcggaaccg catgtccgag cttggcgacc agcgcgcggt agttgtcgac
     2341 cagtgcgacc atcttgcccg gcacgaccgg agtcagcagt ttcgcgtcgg cgagcgaaat
     2401 cgactcaccg gtcggcgtga catgcgcaaa catgtccccg gaatgaacac ggaccgcgtc
     2461 gccttcgata gtgccgaagc attcgcggcc gttgtggttg atgcgcgccc accgtggcat
     2521 cgtttccctt tcgagtagac tcgtcgcgtt atgcggtcgc cgcggccctg agcgcttcga
     2581 cgtcgctcgc gtcgcccaag gtccggcaca catcgaccag tcgcgcggcg cgatcggcgc
     2641 cgagtatcgg gtcgaccaga ccatggaact tgcgctcgag gtcggaatcg ctcattggcc
     2701 gttgcaagct gccgatcgca tgctcgacct taaggtgcaa gcggcggccg tcgttgcagc
     2761 ggatcgtcac gtcggccgcg gtttcgtcga tcgccgcgtc gactttcgca tggactcgat
     2821 cgcgcagcgc gattacctcg gggcggcggg cgatggcatc cgagaattcg gcttcgcccg
     2881 cctggccgaa cagcatcgcc gcggcgcagg cgtggtagac gctgaacttc gcttcgagac
     2941 cgctcttcgg cgcgcgcttg ccggtcagct cgagcaccag cggatggacc agcaaatcga
     3001 tgcgctctac gtcgtcggcg cgcaggcgat gtgcatcgcg gagctggcgg cagccgtcga
     3061 tcgagggatg gatcacgatg ccgcaggcaa acggcttgta ggtgttgaac gaaatctcga
     3121 accgcttgcc gagctcgtcc gagatttcct tccagtcgca tttgctcgag taggtccgca
     3181 gcaggccgcg cggcgcttcg agcgcgcgca tcgaggcggt gtagccgtgc cgtgccatca
     3241 gtgccgaggt gaggcccgcg cgcgcggcgc cgccgatgtg aaacgccttg ctcattgagc
     3301 cgaactgctc tcgcacgccg atcggctgtg acgcggcgat gcccagtgcc atcgccgtct
     3361 tgccggtgtc gagcttgagc atgcgggcgc aggcggcggc agcgcccagc atgccggtcg
     3421 atccggtgat gtgccagccg cgatcgtaat ggtccgggta gatcgcattg ccgatgcggc
     3481 actcgacatc gatgccgagg acaacggcgt cgaccagctc gcggccggtc gtgcgttggc
     3541 gttcagccag cgccagtacg gcggaggcca cggggccggc cggatgaatg atcgtcttca
     3601 gatgcgtatc gtcgaaatcg aacgtgtgcg aggtgatgcc attgagcagc gcggcgctgg
     3661 ctacgtcgac acgctcgctt cgtccgagga tcatcgcttg cgcgccgggc tcgagctcgg
     3721 ccacggcggc cagcgccgct tcgacggtcg gatggcgcga tgcgccgatc gcgcagccca
     3781 cccagttcag caagctccgg tgcgcctcgc ggtcgacgct gtcttcccag ccgcgcgacg
     3841 gatgcgcggc gacgaaatcg gcaaggatgc gggtaatcgg aggagcgtcg agatccgccg
     3901 caatgtgtgc gtcgttggcc atcattcgcc gatctggatg ccgcggtcct ttgcgagctt
     3961 cgaccatttg tcgatgtcgt cacgcatgta ctggccgaat tgctccgacg tcatcggcat
     4021 tggctgcagc gcttcgccgg acagccgctc ggccagatcg gggctctgca gcagcttgcc
     4081 gatctcttcg ttcagccgtt tgacgatcgg ccccggcaag ttcgccgggc cgacgatccc
     4141 gtaccactgc acgccgtcga atcccttgta gcccgactcg tcgaacgtcg gcacctcggg
     4201 cagcagcgga tggcgcctgg ttccggtgac ggcgagcggc ttcatcttgc cggccttgat
     4261 atgcggcagc gcggcggcaa ggccgggaaa cagcgcctgc gtctggccgc cgagtatgtc
     4321 ggtgatcgcc gggccgatgc cgcgatacgg cacgtgcacg atgtcgaggt tggccgcgac
     4381 cttcagctgc tccatcgcga gatgcgtcaa cgttccggga cccgccgagc cgtaggacag
     4441 cttgccggga tttgccctga cgtaagcgac gaattcctgc accgtgctca ccggtatcga
     4501 tggaggaacg atcagcacat tcggcgtgcc agcgatcatc gcgattggcg tgaaatcctt
     4561 gattgcatcg tacggtagct tgcgcacggc cggattggtg ccgtgggtgc cgacgtaccc
     4621 gatcatcaag gtgtagccat cgggggcggc gcgcgcggtg gccagcgatc cgacgacgcc
     4681 tccaccgccg ctctgattgt cgacgacgat aggcacgcca agcgcgacgc cgagccgatc
     4741 ggcgacggtg cgcgcgacca ggtcgacacc gccgcccggc tggaccggcg cgatgatctt
     4801 gatcgtccgg ttcggatagt cgagcggcct ggtctggccg agcgcggtca cgccaggcaa
     4861 ggcgaccgcg atcaaggcga acagccggat caggcccggt attcctgagg aaagcatcgg
     4921 ggctcctgcc gtgaaagacg cccggcgatt gtactgcgac cgtgccgctg gcgcgctgcg
     4981 cccggcggcg ggatccgtgt taccggctcg gacggcgctt tagtcggagg cgagaaaaaa
     5041 atcgacgatg cgcgcgatct gacctcgcat catttcgcgg ccccggaccg tcgtgcagcc
     5101 acagcgttcg gcgagcgcga gcagcggcgt cgtctccggc ttgacgattg catcgaacac
     5161 cgtcatcgca cgaggcagcg acgtgatggc gatcggcagg cgtgcgtcgt cgagcatccc
     5221 cgatggtgtt gcgttcagca ggatgtcgat cccgtcgagc tgcggcgctc cggcgcttac
     5281 ggtgaggccg ggaaatgcac gccctatcct cgcggccagc gcctcggcgc gcgttgcgtc
     5341 gacgtcgtat acccgaagcg acgccggtcg ttcggaagca agcgcgaccc cgatcgccga
     5401 tcccgctccg ccggcgccga gcagcagcgc atgtctgccg atcagaggga tgccgcgtcg
     5461 gcgaaacgca tcgacacatc cgatgccgtc gaacatgtcg ccgcgccagc ccccaggacg
     5521 gcggccgagc gcgttgatcg caccgacgat gcgcgcctgc tctcccaggc gatcggcgag
     5581 cgcgcaagcg cgcagcttgt acggaatagt gaatatgacc ccgtccagat tggagaggcg
     5641 aagcagctgc ggcagcagcg cctcgaagtc gtcgggcagc acatgcagcg gaacgaggat
     5701 ggcgtcgagc gcccggcggc ggaattccgc ggtgaacatc tccggcgagc gcacctgctc
     5761 gatcggatgc ccgacgatgc cgaacagccg cgtccggccc gagatgtagt cgacctgatc
     5821 ggtaaaagtt tgcccggact cactcacgca tattctccgc tgccgtatcg agatactagc
     5881 gcggaaagcc gttacaattg cgcgattcga aaacccatgg actgtccttg tctccgacgc
     5941 ttgccgccat tcgtctgagc tatacggcgc cgtttgccca cgccggctcc gctcgcgcgc
     6001 cggcgccggc atccgcccga aggggatcga atgtcggctg aagtc
//
LOCUS       DATANS010000227         3273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_726394, whole genome shotgun sequence.
ACCESSION   DATANS010000227 DATANS010000000
VERSION     DATANS010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726394"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VHT22_03220"
     CDS             <1..301
                     /locus_tag="VHT22_03220"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEX3136593.1"
                     /translation="FTQSLKLVAYSYTAPWVAGIFLLLGGTIGGLIGLIAAIYAWYTF
                     YLGVPALKKCPPEKAVAYTIVVVLCGIVLAIVLGGVLMSAMFGGAVNGTGFGLMR"
     gene            complement(349..906)
                     /locus_tag="VHT22_03225"
     CDS             complement(349..906)
                     /locus_tag="VHT22_03225"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEX3136594.1"
                     /translation="MLDRDGYRPNVAIVVSNSKNLVFWGKRIREHSWQFPQGGINPGE
                     TPEQAMYRELKEEVGLEPDHVKILGRTRDWLRYEVPQHWIKREWRGSYRGQKQIWYLL
                     RLTGRDSDVSLRASEHPEFDAWRWHDYWIPLENVIEFKRDVYRRGLTELERFLHQRPQ
                     TRRERLYGWAHSVVPSPYTNPTGKA"
     gene            complement(1003..2034)
                     /locus_tag="VHT22_03230"
     CDS             complement(1003..2034)
                     /locus_tag="VHT22_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX3136595.1"
                     /translation="MSPLDGHAGPHLHLVLDHASAAAPAEREGAVDDAALLDAYSAAV
                     VGVVERVRPAVVHIRVERPGKGRGEGSGSGFIIAPDGYLLTNSHVAGGASALEVTLPD
                     GRTASAELVGEDPDSDLAVVKVGAPELAYSRLGDSTRVRVGQIAIAIGSPYGFQHTVT
                     AGIVSALGRSMRSQTGRLLDNIIQTDAALNPGNSGGPLVSAAGDVIGVNTAMILPAQG
                     ICFAIAANTAERVAVALMREGRVRRAYLGVGGHDTPIARRIVRHFGLGHESAVRVSTV
                     EKDSPAAAAGIRTGDLIIGFGDEAIKGIDDLQRLLTGRRIGDAVDVVVLRRDQRLLLR
                     TTPRELSRR"
     gene            2261..2695
                     /locus_tag="VHT22_03235"
     CDS             2261..2695
                     /locus_tag="VHT22_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX3136596.1"
                     /translation="MTDIRATTSQDPQSVGTAVLGGGPAGLAARPELMPAHALKGDPV
                     VNPKGYALGRVEHIVLDVQLGRIAYAVLTFGGFLGMGEKLFAIPWTAFTFDADRHRFI
                     LNVDRGVLEGAPGFDKKHWPAMADPDWATHLHAYYGYRPYWD"
     gene            complement(2703..>3273)
                     /locus_tag="VHT22_03240"
     CDS             complement(2703..>3273)
                     /locus_tag="VHT22_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX3136597.1"
                     /translation="ITALPVDAFVCLRGSKYCQSEEVFNLACRLFGGLAPGFSRVDAI
                     CRWIRANIDYLIGTSTPSYSARDVLALRAGVCRDFAHLAITFCRALNIPARFVTGYAR
                     YAEPPPDFHAVFEAFLGGRWQLFDATELSPMRDLVRIGTGRDASEVPFATFFGTARLR
                     RLSALIEAAPEGAGLMRLQTPTSGIQLAA"
BASE COUNT          572 a         1074 c         1067 g          560 t
ORIGIN      
        1 cttcacccag tcactgaagc tcgtcgcgta ctcgtacacg gcgccctggg tcgccgggat
       61 cttcctgctg ctcggcggca cgatcggcgg cctgatcggc ctcatcgccg cgatctacgc
      121 ctggtatacg ttttatctgg gcgtgccggc gctgaagaaa tgcccgccgg aaaaggccgt
      181 cgcctacacg atcgtcgtcg tgctgtgcgg catcgtgctg gcgatcgtcc tcggcggcgt
      241 gctgatgtcc gccatgttcg gcggagctgt gaacggtacg ggattcggac taatgcgctg
      301 agcaggggcc gggcaggccg ggccccgcgc atctggcgtc gtgcgacgtc aggccttgcc
      361 ggtcggattg gtgtagggcg acggcacgac gctgtgcgcc cagccataca ggcgctcgcg
      421 ccgcgtttgc ggccgctggt ggaggaaacg ctcgagctcg gtcagcccgc ggcgatagac
      481 gtcgcgcttg aactcgatga cgttctcaag cggaatccag tagtcgtgcc agcgccaggc
      541 atcgaattcc ggatgctcgc tggcgcgcag actcacgtcg ctgtctcggc cggtcaggcg
      601 caacagatac cagatctgct tctggcctcg gtaactgccg cgccattcgc gcttgatcca
      661 gtgctgtgga acctcgtagc gcagccagtc gcgcgtgcgt ccgaggatct tgacatggtc
      721 gggctcgagg ccgacctctt ccttcaactc ccgatacatc gcctgctcgg gcgtctcgcc
      781 gggattgatc cccccttgcg gaaactgcca cgagtgctcg cggatccgct tgccccaaaa
      841 gaccagattc ttcgagttcg agacgacaat ggcgacgttc gggcgatacc cgtcacggtc
      901 cagcatcgcg acacctgtag ttctgcgcgt tgcggatatt cttgcatagt tcggcggggt
      961 tgaaaagcgc gccccccccg ccgtggacgg cgcgtcgaag tgtcagcggc gcgacagctc
     1021 gcgcggcgtt gtgcgcagca gcaggcgctg atcgcggcgc agtacgacca cgtcgaccgc
     1081 gtcgcctatg cgccgtcccg tgagcaaacg ctgcaagtcg tcgatgcctt tgatcgcttc
     1141 gtcaccgaaa ccgatgatca ggtcgccggt gcggattccg gccgccgcgg cgggcgaatc
     1201 cttctcgacc gtcgagacgc gcacggccga ttcgtggccc agaccgaaat gacgaacgat
     1261 gcgccgcgcg atcggcgtat cgtggccacc gacgccgaga tacgcgcgtc gcaccctgcc
     1321 ctctcgcatc agcgccaccg cgacgcgctc ggcggtattg gccgcgatcg cgaagcagat
     1381 cccctgagca ggcaggatca tcgcggtgtt gacgccgatc acatcgccgg ccgcactcac
     1441 cagcggcccc cccgaattgc ccgggttgag cgccgcgtcg gtctggatga tgttgtcgag
     1501 cagccggccg gtctggctgc gcatcgaccg cccgagtgcg ctcacgatac ccgcggtgac
     1561 cgtgtgctga aatccgtagg gactgccgat tgcgatcgcg atctgtccga ctcggacccg
     1621 cgtggaatcg cccagtcgcg agtaggccag ctccggcgca ccgactttga cgaccgcgag
     1681 atcggaatcg ggatcttcgc cgaccagctc ggccgacgcg gtgcgtccgt cgggcagtgt
     1741 gacttcgagc gcgctcgcgc cacccgcgac atgactgttg gtcagcagat atccgtccgg
     1801 cgcgatgatg aagccggagc ccgagccttc gccgcgtccc ttgccggggc gctcgactcg
     1861 aatgtggacg accgctgggc gcacgcgctc gaccaccccg acgactgcgg ccgaataggc
     1921 atcgagcaac gccgcatcgt cgaccgcgcc ctcgcgctcg gccggcgctg cggcggaggc
     1981 atgatcgagc accagatgca gatgcggacc agcgtgaccg tcgagtggcg acatggcagg
     2041 tagttggcgg tgcgcgccgg gaattcaagc ggcctggccg ccagccggcg gggtcacgct
     2101 cccgaacagc gcggaccgct ttctttgcaa ggcctactac cagttgcatt tcttgcccgc
     2161 aaccgccgat actttcggtg ctggacgatg cgattgcggc acgaattcga tggcacgtcg
     2221 attgcttgag ttgacgctgg accgaatggg gagacgaacg atgaccgaca tccgggcgac
     2281 cacctcgcag gacccgcaat cggtaggcac ggcggtattg ggcgggggcc cggccggctt
     2341 ggccgcgagg cccgaattga tgccggcgca tgcgctcaag ggcgaccccg ttgtcaatcc
     2401 caaaggttat gcgctgggta gagtcgagca catcgtcctc gatgtccaac tcggtcgcat
     2461 cgcctacgcg gtgctcactt tcggcgggtt cctcggaatg ggcgaaaagc tgttcgcaat
     2521 tccgtggacc gcttttacat tcgacgctga ccgccatcgc tttattctca acgtcgaccg
     2581 cggcgtgctc gaaggcgcac ccggattcga caaaaaacat tggccggcga tggccgatcc
     2641 ggattgggcc acgcatctgc acgcctacta cggctatcgc ccgtactggg actagccgga
     2701 tttcaggcgg ccagctggat gcccgacgtg ggcgtctgca ggcgcataag acccgcgcct
     2761 tccggcgcag cctcgatcag cgcactcaat cggcgcagcc gcgccgtccc gaaaaaagtc
     2821 gcgaaaggga cttccgacgc atcgcgcccg gtgccgatac gcaccaggtc ccgcatcggc
     2881 gagagctcgg tcgcatcgaa caactgccat ctaccgccga ggaaggcctc gaataccgcg
     2941 tgaaaatcgg gcggaggctc ggcgtagcgc gcataaccgg tgacaaagcg cgcaggaatg
     3001 ttcaacgcgc ggcaaaaagt gatcgcgaga tgcgcgaaat cgcggcagac gccggcgcgc
     3061 aaggcgagga catcgcgcgc cgagtagctc ggcgtcgatg tcccgatcaa gtagtcgatg
     3121 ttggcgcgta tccagcggca gatcgcgtcg acgcgcgaaa agcccggcgc gaggccgccg
     3181 aatagccggc acgcaagatt gaagacttcc tcggattggc aatacttgct gccgcgaagg
     3241 cagacgaacg catcgacggg caaggcggta atc
//
LOCUS       DATANS010000228         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_726419, whole genome shotgun sequence.
ACCESSION   DATANS010000228 DATANS010000000
VERSION     DATANS010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726419"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="VHT22_03245"
     CDS             <1..129
                     /locus_tag="VHT22_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010970615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3136598.1"
                     /translation="VYVDDRTIDSHIKRLRKKFKVVDDDFEMIETLYGVGYRFKEA"
     gene            231..>1574
                     /locus_tag="VHT22_03250"
     CDS             231..>1574
                     /locus_tag="VHT22_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX3136599.1"
                     /translation="MLDRTQTDPSPNNEDASELLEQRNAADDSPGEKGWRRPLGWLRR
                     AGQFFFALSFSSLTRRIVSLNLAGLVALVASILYLSQFRAGLIDARAQSLLVQAEIIA
                     GAIAASATVETNTITIDPDRLLDLKPGESYGAPDESSGLDFPINPERVAPVLRRLISP
                     TKTRARIYGGDGGMILDSRSLYGRGDVLRFELPPPSTEKPGFVERATISIRTWLNRGD
                     LPLYRELGPENGKGYQEIVQALDGVKSSVVRVNDRGEVIVSVAVPVQRFRAVHGALML
                     STQGDDIDQMVTAERLAILKVGGVASAVMIVLSLLLASTIAGPVRRLADGAERVRRRI
                     QTRVEIPDFTRRRDEIGHLSGALRDMTDALYNRIEAIEMFAADVAHELKNPLTSLRSA
                     VETLPLARNDTSRGRLLEVIEHDVKRLDRLISDISDASRLDAELQRQDMAPVNVRR"
BASE COUNT          273 a          541 c          501 g          259 t
ORIGIN      
        1 gtctatgtcg acgaccgcac catcgacagc cacatcaagc ggctgcgcaa aaagttcaag
       61 gtggtcgacg acgatttcga gatgatcgag acgctgtacg gcgtcggcta tcgcttcaag
      121 gaagcctgat ctgcgttttc cgggcggacg tgccgactga cacatccgcc cgttcggcgt
      181 agaacgcgta agaattaata agcggcgcat cacaaccggc agctcggcca ttgctagatc
      241 gaacgcagac cgatcccagc ccgaacaacg aggatgcttc ggagctcctc gagcagcgca
      301 acgctgccga cgatagcccg ggcgaaaagg gctggcggcg gccgcttggc tggttgcgcc
      361 gggccgggca attcttcttc gcgctctcct tctccagcct cacgcgccgg atcgtctcgc
      421 tcaacctggc gggcctcgta gccctggtgg cgagcattct ctatctctcg cagttccgcg
      481 ccggcctgat cgacgcgcgc gcgcagagcc tgctggtgca ggccgaaatc atcgccggcg
      541 cgattgccgc ctccgccacc gtcgaaacca acaccatcac catcgatccg gaccgcctgc
      601 tcgacctcaa gcccggcgaa agctacggcg cgccggacga atcctcaggt cttgattttc
      661 cgatcaatcc ggagcgcgtc gcgccggtgc tgcggcgact gatctcgccg accaagacgc
      721 gggcacgcat ctatggcggc gatggcggca tgatcctcga cagccgcagc ctctatggcc
      781 gcggcgacgt gctgcggttc gaactgccgc ctccctcgac ggagaagccg ggctttgtcg
      841 agcgcgccac gatctcgatc cgcacgtggc tcaatcgcgg cgacctgccg ctgtatcgcg
      901 aactcggccc cgagaacggc aagggctacc aggaaatcgt gcaggcgctc gacggcgtca
      961 aaagcagcgt ggtgcgcgtc aacgaccgcg gcgaggtgat cgtctcggtc gctgtccccg
     1021 tgcaacgctt ccgcgccgtg cacggcgcgc tgatgctttc cacgcagggc gacgacatcg
     1081 accagatggt gaccgccgag cggctggcga tcctcaaggt cggcggcgtc gcttccgcgg
     1141 tcatgatcgt gctgtcgctc ctgctggcga gcacgatcgc gggtccggtg cggcggctgg
     1201 ccgacggcgc cgagcgcgtc cgccggcgca tccagacccg cgtcgagatt cccgatttca
     1261 cccgccgccg cgacgagatc ggccatcttt ccggcgcgct gcgcgacatg acggatgcgc
     1321 tctataaccg catcgaggcg atcgagatgt tcgccgccga cgtcgcccat gaattgaaga
     1381 acccgctgac ctcgctgcgg tcggcggtgg aaacgctgcc gctggcgcgc aacgacacca
     1441 gccgcgggcg cctgctcgag gtgatcgagc acgacgtcaa gcggctcgac cggctgatct
     1501 cggatatttc cgacgccagc cgcctcgacg cggaattgca gcgccaggac atggcgccgg
     1561 tcaatgtgcg gcgg
//
LOCUS       DATANS010000229         2921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_11259, whole genome shotgun sequence.
ACCESSION   DATANS010000229 DATANS010000000
VERSION     DATANS010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11259"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VHT22_03255"
     CDS             complement(<1..823)
                     /locus_tag="VHT22_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136600.1"
                     /translation="MTGRPTQEPGDGNIGLYRAADFRLTDGRCADCAAIPQALWYFRD
                     EAIAVPRPGLPVAGFTRGASTFDDVARWAASHAPGTAPDYPTLIWVASPAVLRGARLD
                     AAGAHIAVAGTTTAFEVVPKIPLNRSYYNAASVAYFTGRTLKMRGEIAEGKFTARSIW
                     PEDFRLDVNDAAASSPEAAADPSPEAVRKLLRAEPRGGATSGFAAITLWERTPGAARR
                     CVGKPVLAAMLNGAQGDDDEAHGGHFALVTGRVGADGGIDDWMANNFYTLDLFSEK"
     gene            complement(820..>2921)
                     /gene="mdoH"
                     /locus_tag="VHT22_03260"
     CDS             complement(820..>2921)
                     /gene="mdoH"
                     /locus_tag="VHT22_03260"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEX3136601.1"
                     /translation="LRRIVLMGLIVSQTYIASNFMVAVLPYHGRQPLEIAILILFAIL
                     FGWISAGFWTAMAGFVLLVFGSDRYAITDGTPRNTAIDPKARVAIIMPICNENVSRVF
                     AGLAASYESLARTGDLKHFDFFVLSDTSKPDIRVAELDAWLALCRAINGFDRVFYRWR
                     QHRIKRKSGNIADFCRRWGNRYRYMVVLDADSIMSGACLTALLRLSESNPDVGIIQTA
                     PHAAGRETLYARVQQFATSVYGPLFTAGLHFWQLGESHYWGHNAIIRVAPFMRHCALG
                     RLPGHGTLSGEILSHDFVEAALMRRAGWQVMIAYDLDGSYEEMPPNLVDELTRDRRWC
                     RGNLMNFRLFLMRGLHAAHRAVFMSGVMAYLSALLWFLSLILSTALLAVHALSAPKYF
                     VRPFQLFPLWPEWHPEWAIALFSATAILLFLPKVLGALLIVVRGAAEYGGRARLCLSL
                     LGEMLISAMLAPIRMLFHSQFVVGAFAGWAIHWQSPTREDAETTWREALRRHGAHTLL
                     GTLWAAGVYWLNPSFLWWLLPIVGALMLSIPLSVYTSRLRLGRRLRAAGLFLIPEEAR
                     PPAEIRAMMQAVASAPMPPGFVDAVIDPATNALACAAAVARFTRTALVQNQRMQKVDV
                     ALVEGPDGLSASSKLRLLGDPIALSRLHWRVWTSPDAHVVWRDAIASARRTAVVVATP
                     AHRLVRADLRSPARVWS"
BASE COUNT          555 a          946 c          979 g          441 t
ORIGIN      
        1 ctttttcgct gaacagatcg agcgtataga aattgtttgc catccagtcg tcgatcccgc
       61 cgtcggcgcc gacgcggccg gtgacgagcg cgaagtggcc gccgtgggct tcgtcgtcgt
      121 cgccctgagc gccgttgagc atcgcggcga gcaccggctt gcccacacag cggcgcgccg
      181 cgcccggcgt gcgctcccac aaggtgattg cggcgaaccc gctggtcgcg ccgccccgcg
      241 gctcggcgcg caaaagcttc cgcaccgcct cgggcgatgg gtctgcggca gcctcgggcg
      301 acgacgcggc ggcgtcattc acgtcgagcc ggaaatcctc cggccatatc gagcgggcgg
      361 tgaacttgcc ttcggcgatt tcgccgcgca tcttgagcgt cctgccggtg aaataagcga
      421 ccgaggctgc gttgtaatac gaccgattga gcgggatctt gggcacgacc tcgaacgccg
      481 tcgtcgtgcc tgcgaccgca atgtgcgcac ccgctgcgtc gagtcgcgcg ccgcgcagca
      541 ccgccggcga ggcgacccag atcagcgtcg gataatcggg cgcggttccc ggcgcatggc
      601 tcgccgccca gcgcgcgacg tcgtcgaacg tgcttgcgcc gcgggtgaat cctgcaacag
      661 gtagcccggg tcgcggtacg gcgatcgcct catcgcggaa ataccacagc gcctgcggta
      721 ttgcggcgca atcagcgcag cgcccgtcgg tgagccggaa gtcggcggcg cgataaagac
      781 cgatgttgcc gtcgccaggc tcctgcgtcg ggcgaccggt catgaccaga ccctggctgg
      841 cgatcgcagg tcggcgcgga ccaggcggtg tgccggcgtt gcgacgacga cagccgtgcg
      901 gcgcgcagac gcgatagcgt cccgccacac gacatgggca tcgggcgagg tccatacgcg
      961 ccaatgcaga cgcgacagcg cgatcgggtc gccgagcaag cggagtttcg aggatgcgct
     1021 caagccgtca ggtccttcga ccagcgccac gtcgaccttt tgcatccgct ggttctggac
     1081 cagggcagtt cgcgtaaatc gggcgacggc ggcggcgcag gccagtgcgt tggtagcggg
     1141 gtcgatgacc gcatcgacaa agcccggtgg catcggcgcg gaggcaaccg cctgcatcat
     1201 cgcgcgtatc tccgccggcg gccgcgcttc ttccggaatc aggaacagcc cggccgcgcg
     1261 cagtcggcgc ccgaggcgga gtcggctcgt atagacggaa agcggtatcg aaagcatcag
     1321 cgcaccgacg atgggcagca accaccagag gaacgacgga ttgagccaat acacgccagc
     1381 ggcccagagc gtgcccagca gcgtgtgcgc cccgtgtcgc cgcagcgctt cacgccacgt
     1441 ggtttcggcg tcctcgcgcg tcggcgactg ccagtggatt gcccacccgg caaaagcgcc
     1501 gacgacgaac tgcgagtgga acagcatccg tatcggcgcg agcatcgccg atatcagcat
     1561 ctctccgagc agactcaagc acaggcgcgc gcgccctccg tactcggctg cgccgcgcac
     1621 gacgatgagc agggcgccga gcaccttcgg cagaaacagc agaatcgccg tcgccgaaaa
     1681 gagcgcgatg gcccattccg gatgccactc cggccaaagc ggaaagagct ggaagggccg
     1741 cacgaaatac ttgggcgcgc tcagagcgtg aaccgccagc agggccgtcg acaggatcag
     1801 cgaaagaaac cacagcaggg cggaaaggta ggccatgacg ccgctcatga agaccgcgcg
     1861 gtgggcggcg tgaaggccgc gcatcaggaa caggcggaaa ttcatcagat tgccacggca
     1921 ccagcgccgg tcgcgcgtaa gctcgtcgac caggttcggc ggcatttcct cgtagctgcc
     1981 gtcgagatcg taggcgatca tgacctgcca tccggcccgt cgcatcagtg ccgcctcgac
     2041 gaaatcgtgc gaaaggattt ctccggacag cgtgccgtgg cccggcagac gaccgagcgc
     2101 gcaatgccgc atgaacggag cgacgcgaat gatcgcgttg tgcccccaat aatgcgattc
     2161 gccgagctgc cagaagtgca ggccggcggt gaacagcggc ccataaacgc tggtagcgaa
     2221 ctgctgaacg cgggcatata gcgtttcgcg tccggccgca tgcggcgcag tctggatgat
     2281 gccgacatcg gggttcgatt cgctcagccg caacagcgcg gtgaggcaag ctccgctcat
     2341 gatgctgtcg gcgtcgagca cgaccatgta gcggtagcgg ttcccccagc gccggcagaa
     2401 atcggcgatg ttgccgctct tgcgcttgat ccgatgctgc cgccaccggt aaaacacccg
     2461 gtcgaagccg tttatcgcgc ggcacagcgc cagccacgca tcgagctccg cgacgcgtat
     2521 gtccggcttg ctggtgtcgg agagcacgaa aaaatcgaaa tgcttcaggt ctccggtgcg
     2581 ggccagcgat tcgtagctgg cagccagccc cgcgaagacc cgcgacacgt tttcgttgca
     2641 gatcggcatg atgatcgcga cccgggcttt gggatcgatc gcagtattgc ggggcgtgcc
     2701 atcggtgatc gcgtagcgat cgctgccgaa caccagcagg acgaagccgg ccattgcagt
     2761 ccagaaaccc gccgaaatcc agccgaagag aattgcgaag agtatgagga tcgcgatttc
     2821 cagtggctgg cggccgtgat acggcagcac ggcgaccatg aaattgctcg cgatataggt
     2881 ttgcgacacg atcaggccca tcaggacgat gcgccggagc g
//
LOCUS       DATANS010000230         1515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_767160, whole genome shotgun sequence.
ACCESSION   DATANS010000230 DATANS010000000
VERSION     DATANS010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767160"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VHT22_03265"
     CDS             complement(<1..720)
                     /locus_tag="VHT22_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEX3136602.1"
                     /translation="MSATDTAGLTKQGYDPVALADSLASAADKSAKLLGDYLSRQSKS
                     GPSLAGDEFGLTKAFMEMAAKMLSNPYRMAETQMNLWWDYSALWQSSMLKLMGQTSVP
                     VAEPMKGDRRFRHEDWQEHFLFDYVKQSYLIAARWLHNAVTSVEGLDEHTQKKVDFFT
                     RQYIDALAPSNFALTNPEVFRETIATGGQNLVRGLHNLLDDIERGNGQLKISMTDTKA
                     FELGVNIATTPGQVVFQNDLIQ"
     gene            complement(742..>1515)
                     /locus_tag="VHT22_03270"
     CDS             complement(742..>1515)
                     /locus_tag="VHT22_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3136603.1"
                     /translation="PDHLGNAAWLAARFCCPVMMPQAEYLTAHAIAGDHSGYGAASMR
                     EMLRAHGLPAEHLAALDARGNSYRRGVPEIPTSFQRLAAHDAVRIGANDWQVIPGSGH
                     SPEHASLYCKDLGVCISGDMLLPRISTNVSVWPIEPEGDPLRRFLDSLTAFTELPGET
                     LILPSHGLPFVGAGARVDQLREHHAARLAELEIAATTPKTAADIVPVLFRRELDLQQR
                     FFAMGEAIAHLNHLWHRGRLDRSVGDDGTIRFLRAAGIQ"
BASE COUNT          263 a          465 c          504 g          283 t
ORIGIN      
        1 ctggatcagg tcgttctgga acacgacctg gccgggcgtg gtcgcgatgt tgacgccgag
       61 ctcgaacgcc ttagtgtccg tcatcgagat cttgagctgg ccgttgccgc gctcgatgtc
      121 gtccagcagg ttgtgcaggc cgcgcacgag gttctggccg ccggtggcga tggtctcgcg
      181 gaacacctcg ggattggtga gcgcgaagtt cgacggcgcg agcgcgtcga tgtactggcg
      241 agtgaagaaa tcaacctttt tctgcgtgtg ctcgtcgagt ccctcgacac ttgtgacggc
      301 gttgtgcagc cagcgcgccg cgatgaggta cgactgcttg acgtagtcga acaggaaatg
      361 ttcctgccag tcctcgtggc gaaagcgccg gtcgcctttc atcggctcgg cgaccggcac
      421 cgacgtctgg cccatcagct tgagcatcga cgactgccag agcgccgaat aatcccacca
      481 caggttcatt tgcgtctcgg ccatgcggta gggattggac agcatctttg ccgccatttc
      541 catgaatgcc ttggtcagtc cgaattcgtc gccggcgagc gagggccccg acttcgactg
      601 gcggctgagg tagtctccga ggagctttgc gctcttgtcg gcggcgctgg cgagcgagtc
      661 cgccagcgcg acggggtcat agccctgctt ggtcaaaccc gcggtatcgg ttgcgctcat
      721 caaaaccctt tcgaagtgac tttattgtat gccggcagcg cggaggaagc ggatcgtccc
      781 gtcgtcgccg actgagcgat cgagacgccc gcgatgccaa agatggttca gatgggcgat
      841 tgcctcgccc atcgcgaaaa aacgttgttg caagtcgagc tcgcgacgga acagtaccgg
      901 cacgatatcc gccgcggtct tcggcgtcgt tgcggcgatt tcgagctccg ccagccgcgc
      961 ggcgtgatgc tcgcgcagtt gatcgactcg agcgccggca ccgacgaacg gcaacccgtg
     1021 cgagggcagt atcagcgtct cgccgggaag ctcggtgaat gccgtcagcg aatccaggaa
     1081 ccgccgcaac gggtcgcctt cgggctcgat cggccagacg ctgacgttgg tgctgatgcg
     1141 tggcagcagc atgtctccgg aaatgcagac gccgagatcc ttgcagtaga gggaggcgtg
     1201 ctcgggcgaa tggccggagc cgggaatcac ctgccagtca ttcgcgccga tgcggactgc
     1261 atcgtgcgcg gccaggcgct gaaagctcgt tgggatttcc ggcacgccgc gtcggtagga
     1321 attgccgcgg gcatcgagcg ccgccagatg ctcggccggc aagccatgcg cgcgcaacat
     1381 ctcgcgcatc gacgccgcgc catagccact gtggtcgcct gcgatcgcat gcgcggtcag
     1441 atattcggcc tgcggcatca tcaccgggca gcagaagcgc gccgccagcc aggccgcatt
     1501 gcccagatga tcggg
//
LOCUS       DATANS010000231         1966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_726495, whole genome shotgun sequence.
ACCESSION   DATANS010000231 DATANS010000000
VERSION     DATANS010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726495"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..704)
                     /gene="pyrE"
                     /locus_tag="VHT22_03275"
     CDS             complement(60..704)
                     /gene="pyrE"
                     /locus_tag="VHT22_03275"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX3136604.1"
                     /translation="MPDFRQEFLEFALARDVLRFGAFVTKAGRKTPYFFNAGLFNDGA
                     SLGELGRYYATAYLASEVRCDQLYGPAYKGITLAAVTAVALAAKGHNLPYSFNRKEAK
                     DHGEGGAIVGAALKGRVLIVDDVITDGGAKREAIELIRAHGATPAGVLIAFDRMERGR
                     GGRSAVQELEQEFGIPVIAIATLDDLLGLLAGRQDLAQQRREVISYREQYGAAA"
     gene            838..1611
                     /locus_tag="VHT22_03280"
     CDS             838..1611
                     /locus_tag="VHT22_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX3136605.1"
                     /translation="MRIVTLNVNGIRSAERKGLVRWLSRIEPWDVVCLQEIKAHPDDV
                     PKALRAPRKSHAAFHHAQKKGYSGVALYARRAVPVRSGFGSNEFDAEGRYLEADFGKL
                     TVISVYLPSGSSSPDRQLAKFRFLDQFLPHLERLRNAGREVILCGDWNIAHQPIDLKN
                     WRSNQKNSGFLPEERAWLTRVFDELGFVDVFRRINREPDQYTWWSNRGQAWAKNVGWR
                     IDYQIATAGIAATARTAAIYTNRRFSDHAPLIIDYDYLL"
BASE COUNT          342 a          668 c          608 g          348 t
ORIGIN      
        1 aacgtaatcc gctaagtttc acaataagcc ttttcacgtt tccggtgatt ggcgccgcct
       61 cacgcggcgg cgccatattg ctcgcgatac gaaatcactt cgcgcctttg ctgcgccagg
      121 tcctggcgcc ccgccagcaa ccccagcaag tcgtcgagcg tcgcgatcgc tatgaccggg
      181 atgccgaatt cctgttccaa ctcctgcacc gccgaccgcc cgccgcgtcc gcgttccatt
      241 cgatcgaagg cgatcagcac gccggcgggg gtcgccccgt gggcccggat caactcgatc
      301 gcctcgcgct tggccccgcc gtcggtaatg acgtcgtcga cgatcagcac cctgcctttg
      361 agcgcggccc cgacgatcgc gccgccctcg ccgtgatcct tggcttcctt gcgattgaag
      421 ctgtacggca gattgtgacc ttttgcggcg agggcgacag ccgtcaccgc ggccagcgtt
      481 atgcctttgt aggccgggcc gtacagttga tcgcaacgca cctctgaggc cagatacgcc
      541 gtggcgtagt accgacccag ctcgcccaaa ctcgctccgt cgttgaacag ccccgcgttg
      601 aaaaaataag gtgtcttgcg gcccgccttg gtgacgaacg caccaaatcg aagcacgtcg
      661 cgtgccaagg cgaactcgag aaactcctgg cggaaatcgg gcatgaagag cgttgcgcgc
      721 cacaggggat cggccgcaaa ttataccgcg cgacctcgtc gccagcgccg acgcggcggg
      781 agcgggccct cgtgtaaact ccgcgccgcg gcggcgtccg agccggggcc actcgacatg
      841 cgcatcgtca cactcaacgt caatggcatt cgctcggcgg agcgtaaagg tctcgtccga
      901 tggctgtcgc ggatcgagcc gtgggacgtc gtgtgcttgc aggagatcaa ggcccatccc
      961 gacgacgtgc cgaaggcgct gcgggcaccg cgaaaatccc acgccgcgtt tcatcacgcg
     1021 caaaaaaagg gttacagcgg cgttgccttg tacgcccgcc gcgcggtgcc ggtgcgcagc
     1081 ggtttcggca gcaacgagtt cgacgccgag ggccgctatc tggaggccga cttcggcaag
     1141 ctcaccgtga tcagcgtcta tctgccgtca gggtcgagct cgccggaccg ccagctcgcc
     1201 aagttccgtt tcctcgacca gtttctgccg cacctcgagc ggcttcgcaa cgccggacgc
     1261 gaagtgatcc tttgcggcga ctggaacatc gcgcaccagc cgatcgacct caaaaactgg
     1321 cgcagcaacc agaagaattc cggattcctg cctgaagagc gggcgtggtt gacgcgggtg
     1381 ttcgacgagc ttggctttgt cgacgtcttt cgccggatca atcgcgaacc cgatcaatac
     1441 acctggtggt cgaatcgcgg ccaggcctgg gcgaagaacg tcggttggcg tatcgattac
     1501 cagatcgcga cggcaggcat cgccgcgacc gcgcggacgg cggcgatcta cacaaatcgc
     1561 cgattctccg atcacgcacc cttgatcatc gactacgact acctgctttg actgaccttc
     1621 ggcgatcggc cagcccgccg cgatcgcccc gccgacccgt ccccttcgca gcgccttggg
     1681 gcgtgcccga catctgatcg ggacgcgctt tgactccggc cttgcacttt gcggcaggag
     1741 cgccgcgatc gtacgccacg atcgagggca tgcttcttgc ggctaggcgc agccgaaggg
     1801 agcaagaaaa tcaatggtat tcgagggggg cctcacagga cggcatggcc aggcgcgcgc
     1861 gcggcttcgg ctgctcgcgc tggtctgcgc ttgccttgtc gccgcgtcgg cgcaggccga
     1921 cctcaccgtg ccggccggcg gccagctctc gcttggcccg gggagc
//
LOCUS       DATANS010000232         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_221640, whole genome shotgun sequence.
ACCESSION   DATANS010000232 DATANS010000000
VERSION     DATANS010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221640"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VHT22_03285"
     CDS             complement(<1..260)
                     /locus_tag="VHT22_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136606.1"
                     /translation="MKNTLNTLAYWIWKTMLANGQLKLRLIRFSQRFRFVTEVAMRKF
                     IIAAASAIALSFAAVPALAQHGGGGHSGGGHSGGGWSGGGGS"
     gene            390..791
                     /locus_tag="VHT22_03290"
     CDS             390..791
                     /locus_tag="VHT22_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136607.1"
                     /translation="MASDPGMRTADMKMDATSLFREEIYTDRAAGTIRVLIPVTRDGG
                     PDQTRATIYTGEAQILTNVGPLPISFDIPAATLGEAVQKYGDAAKEGVERAMRELQEM
                     RRQASSSILVPGASPGLGGGLPPAPGKLKLP"
     gene            complement(814..>1608)
                     /locus_tag="VHT22_03295"
     CDS             complement(814..>1608)
                     /locus_tag="VHT22_03295"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3136608.1"
                     /translation="NRTTRRLSLTESGSVFYQHCVQLLADLDEAEQSASASAITPRGT
                     LRLSASVTFGERHLAPAIGAFIERYPDMRFDVELSDRAVDLVDEGFDAALRIGAIGSQ
                     NLVGRPVGETRLVCCAAPVYLERRGEPKGPEDLAGHECLTYEYASQRNMWPFRERDGS
                     IRRIKVSGSVHANSGRFLEALAVEGRGIAYEPDFIAGPDIRAGRLVPILIGFQPPPST
                     IQLVYPSRRHLSAKVRAFADFLRERFATAEWMLKSPGAPGERRPRR"
BASE COUNT          307 a          546 c          499 g          256 t
ORIGIN      
        1 cagctaccac caccgccgct ccagccaccg ccgctgtggc cgccgccgct gtggccgcca
       61 ccgccatgct gggccaatgc aggaaccgcc gcgaaagata acgcgattgc ggatgcggcc
      121 gcgataatga attttctcat agcaacctcc gtaacgaatc tgaacctttg actaaaacgg
      181 atcaggcgaa gtttcagctg accgttagcc aacattgtct tccaaatcca atacgcaaga
      241 gtgttgagtg tattcttcaa tgtaacagtc tgtaacctct gtccattcca agcctgccaa
      301 ccacgccggc gccccagccg gcgcgcccgg caaagcaccg cggtttttag tatccttagc
      361 cgttgtaatc aacgacaaag gacgttccca tggccagcga tcccggaatg cgcacggcgg
      421 acatgaaaat ggacgcgact tcgctcttcc gcgaagaaat ctacaccgat cgtgccgccg
      481 gcaccattcg cgtcctcatc cccgtgactc gcgatggcgg tcccgatcag acccgggcga
      541 cgatctacac cggcgaagcc cagatcctga ccaacgtcgg tccgttaccg atcagtttcg
      601 acatcccggc ggcaacgctg ggcgaagccg tgcagaagta cggcgacgcg gccaaggagg
      661 gcgtcgagcg cgcgatgcgg gaactgcagg aaatgcgccg ccaggcgtct tcgtcgatcc
      721 tggtgcccgg tgcaagcccg ggcctgggcg gcggtctgcc tccggcgccc ggcaagctca
      781 agctgccctg atcgctccac agagcaccct tcgctagcgc cgcggtcgac gctcgcccgg
      841 tgcgccgggg gacttgagca tccactcggc cgtggcgaag cgctcgcgca ggaagtcggc
      901 gaacgcgcgc accttggccg acagatgccg gcggctcgga tagacgagct ggatcgtcga
      961 tggcggcggc tgaaacccaa tcagaatcgg caccagccgt ccggcgcgaa tgtccgggcc
     1021 ggcgatgaag tcgggctcgt aggcgatgcc gcggccctcg accgccagcg cctcgagaaa
     1081 ccgcccgctg ttggcgtgca ccgagccgga gaccttgatc cggcggatcg acccatcgcg
     1141 ctcgcgaaag ggccacatat tgcgctgcga cgcgtactcg taggtcaggc attcgtgtcc
     1201 ggccaggtct tcggggccct tgggctcgcc acgacgctcg aggtagaccg gcgccgcgca
     1261 gcacaccaga cgggtttcgc ccaccgggcg cccaaccagg ttctggctgc cgatggcgcc
     1321 gatgcgcagc gcggcgtcga atccctcgtc gaccaggtcg acggcgcgat cggaaagctc
     1381 gacgtcgaag cgcatgtcag gataacgctc gataaaagcg ccgattgccg gcgccagatg
     1441 gcgctcgccg aaggtcaccg aggccgacag gcgcaaggtc ccgcgcggcg tgatcgccga
     1501 agcgcttgcc gactgctcgg cttcgtcgag atcggccagc agttgcacgc agtgctgata
     1561 gaacacgctt ccgctctcgg tcagcgaaag ccgccgcgtc gtgcgatt
//
LOCUS       DATANS010000233         1626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_390120, whole genome shotgun sequence.
ACCESSION   DATANS010000233 DATANS010000000
VERSION     DATANS010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390120"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..699
                     /locus_tag="VHT22_03300"
     CDS             247..699
                     /locus_tag="VHT22_03300"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX3136609.1"
                     /translation="MAKIPRPKDFYAGLLFVAFGVVAIIIARDYPLGTAARMGPGYFP
                     RLLGILLIVLGAALSLTALRGHGTALPGWKWRPVSIVLLSVVAFGLVLTHAGLVLSTI
                     GLIVVASTASREFRFRESLLSGVLLAALSVGVFVVGLKLQLPIWPGGG"
     gene            709..>1626
                     /locus_tag="VHT22_03305"
     CDS             709..>1626
                     /locus_tag="VHT22_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX3136610.1"
                     /translation="MDVFGHLALGFGVALTPINLLYALVGVLVGTLIGVLPGIGPVAT
                     IAMLLPTTYGLQPVSALIMLAGIYYGAQYGGSTTSILINMPGETSSAVTCLDGHQMAL
                     KGEAGAALAVAALGSFFAGCVATVAIAAFSPPLSSVALKFGPAEYCSLMVLGLIGAVV
                     LAHGTLLKAIAMVVLGLLLGIVGTDVNSGMARFAFDIPELIDGIGVVAVAMGLFGFAE
                     IVSNLESTEKRAIVAAKVKGLWLTGKQLRQAAPAVLRGTALGSVLGLLPGGGAMLASF
                     AAYTVEKKVAKDPSRFGKGAIEGVAGPESA"
BASE COUNT          233 a          496 c          558 g          339 t
ORIGIN      
        1 cgcgatgcca aggttccgac gttcgaggaa gtcaagccgc aactggccca gcgcatgcag
       61 ggccaggtag tcgacaagta tctgcgcgat ctgcgagcca agaacggctt ctaaagcgcc
      121 ggcgcgactc gatgtcggtg acgagggcag gcagcgacag cctgcccttt ttcttgcgcc
      181 gacacgccgt tggcggcggt ctttgcctgg atgctattct tcgcccttcg cgaagctgaa
      241 cgacccatgg cgaagattcc gcgtccgaaa gacttctatg ccgggctgct gttcgtggcg
      301 ttcggcgtcg tcgcgatcat catcgctcgg gattatccgc tcggtactgc ggcgcggatg
      361 ggccccggtt atttcccgcg tctgctgggg attctgctga tcgtgctcgg cgccgcgctg
      421 tcgctcactg cattgcgtgg tcatggcact gcgctgccgg gatggaaatg gcggccggtc
      481 agcatcgtgc ttctgagcgt tgttgctttc ggtctggtgc tgacgcatgc cggattggtg
      541 ctgtcgacga ttggcctgat cgtcgtcgcc agcacggcga gccgcgaatt tcgattcagg
      601 gagtcgctat tgagcggcgt cctgctggct gcgctgtcgg tcggtgtctt cgtcgtcggc
      661 ctgaagctgc agctcccgat ctggccaggc ggcggctgac gccaaccgat ggacgtattc
      721 ggccacctcg cgcttggctt cggcgtcgcg ctgacgccga tcaacctgct ctacgcactg
      781 gtcggagtgc tggtcggtac gctgatcggt gttctgcccg gaatcggtcc ggtggcgacg
      841 atcgcgatgc tgctgccgac gacctacggg ctgcagccgg tttcggcgct gatcatgctt
      901 gccggcatct actacggcgc acagtacggc gggtcgacga cgtcgatcct gatcaacatg
      961 ccgggagaaa cctcgtcggc agtcacttgc ctggacgggc accagatggc gctcaaaggc
     1021 gaggccggcg ctgcgttggc ggtggcagcg ctgggctctt tcttcgccgg atgcgtcgcc
     1081 acggtggcga tcgccgcgtt ttcgccgccg ttgtcgtcgg tcgctctcaa gttcgggccc
     1141 gccgaatact gctcgctgat ggtgctgggg ctgatcggcg ccgtcgtgct cgcgcacggc
     1201 acgctgttga aggcgatcgc gatggtcgtg ctcgggctgc tgctgggcat cgtcggcacc
     1261 gatgtcaatt cagggatggc ccgctttgca ttcgacattc cggagctgat cgacggcatc
     1321 ggtgtggtcg cggtagcgat gggactgttc ggcttcgccg agatcgtcag caatctcgaa
     1381 agtaccgaaa agcgcgctat cgtggcggcc aaggtcaagg gcttgtggct gaccggcaag
     1441 cagctgcgcc aggcggcgcc tgccgtgctg cgcggcaccg cgctaggatc ggtgctggga
     1501 ctcctgcccg gtgggggtgc gatgctcgcc tcgttcgccg cctacaccgt agaaaagaag
     1561 gtcgccaagg accccagccg cttcggcaag ggagctatcg aaggcgtcgc cggtcccgag
     1621 tcggcc
//
LOCUS       DATANS010000234         5727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_642900, whole genome shotgun sequence.
ACCESSION   DATANS010000234 DATANS010000000
VERSION     DATANS010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642900"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2324
                     /locus_tag="VHT22_03310"
     CDS             <1..2324
                     /locus_tag="VHT22_03310"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX3136611.1"
                     /translation="YLYRRHLFTDRRRGAETSYHYHALFREFLLEELKNLVPAAERKD
                     ATRRAAELLAARGQVSDALALFRDAGEWESMRSLIRAHALEWARQGRAQAVSDWIDAL
                     PPGMRAADPWLEYWSGRAWIFVQPERGRPALERAFEAFRAAGDLRGQALSLNTIVTGY
                     YYEWANFAPIDRWLPEFDRLLAHGAPMLDAESELRAYTAWVIGLLLREPENANLARRA
                     QRLDELIDDESDLNLRVMAASVLFNYINWKTEGESAAALVGRIEPILGKPEVTPLMQV
                     WWRTHLSFWHYVNGRYGESTAVMTDARAIAERYGLEAYMFEIDHADASALVNKGEHAA
                     AKARLELMERGLSPARRMQWPYFYHVRSMLEQRVGHPEASLQDGERALALARELSLPS
                     LQLPHFLARAAQARAATGDWDGALRAADEATALAPEFERAAFEQRRELLQIEVDFEAG
                     ETQRASERLAAVLTGYRAQGQFVFMRSRPDLAARLANFALQHGIETEFVRALIERNAL
                     VAPSDASADWPFRLRIRLLGGFELVRDGHSVTFTGKAQQRPFDLLKLLVALGGVNVDI
                     QQLTAALWPDADGAAAKTSFDSTLFRLRKLLDVENALVLSAGKLSLARSLVWTDVWAL
                     EAAFDAAQPGGRGDGAGAPAERLAARVLDAYPGALLGADEHPWIAKPRDALRARFVRT
                     LMRLGEQLEAKGDWEHAIDVYRRGLEADNLAESFYRGLMRALAATGDQAEALNAFRRC
                     RELLSIVLGIKPSAETDHLYREISAGVRPASRP"
     gene            2556..4676
                     /locus_tag="VHT22_03315"
     CDS             2556..4676
                     /locus_tag="VHT22_03315"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX3136612.1"
                     /translation="MKTAWKNVIDGSKDRWQKAVRQLGIAASFAMFSALPAVAQTYDI
                     TNLGLGGQWSAVSAINAQGQVAGISASADGGVHAFFASQSEGNIDIGTLGEFYSEPHA
                     INASGQIVGDSCRTFGDWTTCRAFSWTKAGGIVALGTLGGDWSTAVGVNDSGKVTGRS
                     QLANGDEHAYSWTQAGGMVDLGTLGGGARYSEPYSINALGQIAGYSYTPDNTRWHAVL
                     WQPNGAIVDLGSLGGDLSWAWAINDAGQVTGTSQWSVNGSYSAQRAFFWSPTSGMVDI
                     GTLGGIYSWPDKINASGQVIGFSLTASNDFRYAQGFSWTLAGGLVDMTLGGAASSVSG
                     ISASGQVVGASSTPSGAPHAFAWTNAGGMRDLGTLGGSSSTAYATNIWGQAVGQAMLA
                     SDSTAHAFLFDGGALKDLNDLAPNKPAGMEFMTAFHLSDSKRLVAWTTAGAVLLSPSS
                     TVAAAPVVGPISANDPVAVGAVVSVSASFTDAATDTHSASWTWGDGTPAGSATVSETN
                     GSGTASGFHTYAAAGIYPVSVAVTDNTGKTGQVSRNVVVYDPSAGFVTGSGWIQSPAG
                     AFKADPTVVGRATFGFVSKYEKGAKVPTGNTEFHFQSANLDFHSDSYDWLVVGGARGQ
                     FKGTGTLNNSGGYQFLLTAVDGALLAGGTAPDRFRIKIWTYDTDLKQDVVVYDNQLSS
                     STEGTTSEGTAIGGGSIVVHTSSK"
     gene            4906..>5727
                     /locus_tag="VHT22_03320"
     CDS             4906..>5727
                     /locus_tag="VHT22_03320"
                     /inference="COORDINATES: protein motif:HMM:NF016552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136613.1"
                     /translation="MNRKLTGLAIVFALVLGAIGTPASAQQGNASTNGGPAKTDSRIL
                     YHNGRVLLGAQTIYVVWYGNWASDINRQIILANFISDLGSSTYFQINNGYPNSAGQAP
                     NGALTYGGAANDAYSHGATLTEADVADVVAQLILTGGATGLPFDASAIYVVLTSPDVT
                     LEDGATHFCLTCCNLHGDSVVAGSPFRWVFVGHPARCPGSCGSQPSGVHTANGDMAAD
                     AMVSWLAHALNAVVTNPENDGWFDRYGLENSEKCEGTYGTTYPVTNLWGESALANV"
BASE COUNT          959 a         1895 c         1913 g          960 t
ORIGIN      
        1 attacctgta ccgccgtcat cttttcaccg accggcgccg cggcgccgag acgagctacc
       61 actaccacgc gctgttccgc gagttcctgc tggaagagtt gaagaacctc gtgccggcgg
      121 ccgagcggaa ggacgcgacc aggcgcgccg ccgagcttct cgccgcgcgc ggacaggtca
      181 gcgatgcgct ggcgctgttc cgcgatgcgg gcgagtggga gtcgatgcgc tcgctgattc
      241 gcgcccacgc gctcgaatgg gcgcggcagg gtcgtgctca agccgtgtcc gactggatcg
      301 atgcgctgcc gccgggcatg cgcgcagccg atccctggct cgaatactgg tcgggtcggg
      361 cgtggatctt cgtccagccg gagcgcggcc ggccagcgct cgagcgcgcc ttcgaagcct
      421 ttcgcgccgc cggcgatctg cgcggacagg cgctgtcact caacacgatc gtgaccggct
      481 actactacga gtgggcgaat ttcgcgccga tcgaccgctg gctgccggag ttcgatcgcc
      541 tgctggcgca tggcgcgccg atgctcgatg ccgaaagcga gttgcgggcg tacaccgcct
      601 gggtgatcgg gctgctgctg cgcgagcccg agaacgcgaa cctcgcgcgt cgcgcgcaac
      661 ggctcgacga gctgatcgac gacgaaagcg acttgaacct tcgcgtgatg gccgcttcgg
      721 tgttgttcaa ttacatcaac tggaagaccg agggcgagtc ggccgcggcc ctggttggtc
      781 gcatcgagcc gatcctcggc aaaccggaag tcacgccgtt gatgcaggtg tggtggcgca
      841 cgcacctgtc gttctggcac tacgtgaacg ggcgctacgg cgagtcgacg gcggtcatga
      901 ccgatgcccg cgcgatcgcc gagcgctacg ggctcgaggc ctatatgttc gagatcgacc
      961 atgcggacgc atccgcgctg gtcaataagg gcgagcacgc ggccgccaag gcgcgcctgg
     1021 agctgatgga gcgcggcttg tcgccggcgc gccgtatgca gtggccgtat ttttatcatg
     1081 tccgctcgat gctcgagcag cgggtcggac atcccgaggc gtcgttgcag gacggggagc
     1141 gggccctcgc gcttgcccgc gagctcagtc ttccgtcgct gcaattgccg catttcctgg
     1201 cccgcgccgc gcaggctcgc gccgcaaccg gcgattggga cggtgcgttg cgcgccgcgg
     1261 acgaagcgac cgccctggcg cccgaattcg agcgcgctgc gttcgagcag cggcgcgagt
     1321 tgctgcagat cgaggtcgac ttcgaagccg gcgaaacgca acgggcctca gagcgactcg
     1381 ccgccgtgct caccggttac cgggcccaag gccaattcgt cttcatgcgc agccgcccgg
     1441 acctcgcagc gcggctcgcc aactttgcgc tgcagcacgg catcgaaacc gagttcgtgc
     1501 gcgcgctgat cgagcgcaac gccctggtcg cgccatccga cgcgagcgcc gattggccgt
     1561 ttcgcttgcg cattcggttg ctcggaggat ttgaactcgt tcgcgacgga cactcggtca
     1621 ccttcactgg caaagcgcag cagcggccgt tcgatctgtt gaagctctta gtggcgctcg
     1681 gcggcgtcaa tgtcgacatc cagcagctga cagccgcgct ctggccggac gccgacggtg
     1741 ctgcggcgaa gacgtcgttc gattcgaccc tgtttcgcct gcgcaagctg ctcgacgtcg
     1801 aaaacgcgct ggtgctgtcg gcgggaaagc tctcgctcgc acgctcgctg gtgtggaccg
     1861 atgtctgggc gctcgaagcg gcgttcgacg ctgcgcaacc cggcggtcgc ggcgacggtg
     1921 ccggcgcgcc ggccgagcgg ctcgccgcgc gcgtcctcga cgcgtatccc ggtgcgctgc
     1981 tcggtgccga cgagcatccg tggatcgcca agccgcgcga cgcgctgcgc gcgcgcttcg
     2041 tgcggacttt gatgcggctg ggcgaacagc tcgaggcgaa aggcgactgg gagcacgcga
     2101 tcgacgtcta ccggcgcggg ctcgaggccg acaacctcgc cgagtcgttc tatcgcggcc
     2161 tgatgcgcgc gctcgcggcg accggtgacc aggccgaggc gctgaacgct ttccggcgct
     2221 gccgcgagct cttgtcgatc gtgctgggca tcaagccttc cgccgaaacc gaccacctgt
     2281 accgcgagat ctccgccggc gtccgtcccg caagccgccc gtagcggcgc gttcccctcc
     2341 tccctccgtc cttcgtttca gcgcgccggc cgccccgccc cggccctgaa ccgtgccgcg
     2401 tcggcgaatc ggtaagcgat ccgcaagtcc cctgcccgca cagtccggtc acgcaacgac
     2461 gcattcctgt tgcggtgagg caatcgagca tcggccgttt ccggcgcacg cgccgcgtgg
     2521 ccgctgtcca ccactgtgtc cggaaagggg gattcatgaa aaccgcttgg aaaaacgtga
     2581 tcgacggaag caaggatcga tggcagaaag cagtgcggca gcttggcatc gccgcatcgt
     2641 tcgcgatgtt ctccgcgctg cccgcagtcg cacagacgta cgacatcacc aatctcgggc
     2701 tgggcggaca atggtcggcg gtctcggcga tcaatgcgca gggacaggtg gcgggcatca
     2761 gcgcctccgc tgacggcggg gttcacgcat tcttcgcgtc gcaatcggag ggcaacatcg
     2821 acatcgggac gctcggcgag ttctactccg aaccccacgc gatcaacgct tcggggcaaa
     2881 tcgtcggcga ctcgtgcagg accttcggtg actggacgac gtgccgcgca ttctcatgga
     2941 ccaaggcggg cggcatcgtc gcactgggaa cgctcggtgg cgactggtcg acggccgtgg
     3001 gggtcaacga ttcgggcaag gtcaccggca ggtcgcagct ggcgaacggc gacgaacacg
     3061 cctactcgtg gacgcaagcc ggcggcatgg tcgatctcgg cacgctgggc ggaggcgcgc
     3121 ggtattccga gccatactcg atcaatgcgc tgggccagat cgccgggtat tcgtatacgc
     3181 ccgacaacac acggtggcac gcggtgctgt ggcagccgaa cggcgcgata gtcgatctcg
     3241 gttcgctcgg cggcgatctg tcctgggcgt gggcgatcaa cgatgcggga caggtcacgg
     3301 gcacctcgca gtggtccgtc aacggcagct attccgctca gcgcgccttt ttctggtcgc
     3361 cgaccagcgg aatggtggat atcgggacgc tcggcggtat ttattcctgg ccggataaga
     3421 tcaacgcgtc gggacaggtc atcggtttct cgctcaccgc ctccaacgat ttccggtacg
     3481 cgcaggggtt cagctggaca ctggccggcg gcctggtcga catgaccctc ggcggtgctg
     3541 cttcgtcggt cagcgggatc agcgcgtcgg gccaagtcgt cggcgcttcc agtaccccca
     3601 gcggcgcgcc gcatgctttc gcgtggacga acgctggggg catgcgggat ctgggcacgc
     3661 tcggcggatc gtcatcgacc gcgtacgcga ccaacatctg gggtcaggcg gtcggacagg
     3721 cgatgctcgc cagcgattcg acggcgcacg ctttcctctt cgacggtgga gcgctgaagg
     3781 acctgaacga tctggcaccc aacaagcccg caggcatgga attcatgacc gcgttccatc
     3841 tgtcggacag caagcggctt gtcgcctgga ccaccgccgg cgcggtactg ctgtcgccga
     3901 gctcgaccgt cgcggcggcg cctgtggtcg gtccgatctc ggccaacgat ccggtcgcgg
     3961 tcggcgcggt cgtctcggtc agcgcaagct tcaccgacgc ggcgaccgat acgcactcgg
     4021 cttcgtggac ctggggtgac ggcacaccgg ccggatcggc gaccgtgtcg gaaaccaacg
     4081 gctcgggaac ggcgtcggga ttccacacct acgcggcggc aggcatctat ccggtcagcg
     4141 tggcagtcac cgacaacacc ggcaagaccg gtcaggtcag ccgcaacgtc gtcgtctacg
     4201 atccgtcggc gggattcgtc accggcagcg gctggatcca gtcgccggcc ggagcattca
     4261 aggctgatcc gacagtggtc ggcagagcga ccttcggctt cgtgtcgaaa tacgagaaag
     4321 gcgcgaaggt tccgaccggc aacaccgagt tccactttca aagcgccaat ctggatttcc
     4381 acagcgacag ctacgactgg ctggtcgtcg gcggcgcgcg cggccagttc aagggcacgg
     4441 gcacgctgaa caacagcggc ggctatcaat tcctgctgac cgcggtcgac ggcgcgcttc
     4501 tcgccggcgg caccgcaccg gatcgcttcc ggatcaagat ctggacctac gacaccgacc
     4561 tgaagcagga cgtcgtcgtc tacgacaacc agttgagctc gagcaccgag ggcacgacgt
     4621 cggaaggcac ggcgatcggc ggcggcagca tcgtggttca tacgtccagc aagtaagcac
     4681 gctcgactag tcaggcacag gggcggccgc aacggccgcc cctctttttt tggccggcgc
     4741 cggtgcgatc gacgccggca cgagcgcggg cgggaggaca tcggcgaatc cgtaagcgat
     4801 ccgcaagtcc ttcgcttgca cagtgggcac gtcctcggcc cacacggctt cgggcagccg
     4861 gcggatctcc cgacggcctt aacccaaacc gaaaggggaa ccgccatgaa caggaagctc
     4921 accggactcg cgatcgtttt cgcgctggtc ctcggcgcga tcggaacacc ggcctcggcc
     4981 cagcagggca acgcatcgac gaatgggggc ccggcgaaga ccgacagcag gatcctctac
     5041 cacaacgggc gcgtcctgct cggcgcccag accatctatg tcgtctggta cggcaactgg
     5101 gcgtcggaca tcaatcggca aatcattctg gcgaacttca tatcggatct cggatcgtcg
     5161 acctattttc agatcaacaa cggctatccc aacagcgccg gccaagcgcc aaacggtgcg
     5221 ctgacctacg gcggcgccgc caacgatgcg tattcgcacg gtgccacgct gaccgaggcg
     5281 gacgtcgcgg acgtcgtcgc gcaattgatc ctcaccggag gagccacagg gctccccttc
     5341 gatgcttccg caatctacgt cgtcctgacg tcgcccgatg tcacgctgga agatggtgcg
     5401 acgcactttt gccttacctg ctgcaacctt cacggcgact cggtagtcgc cggttcgccg
     5461 ttcaggtggg tgttcgtcgg tcatccggcg cgctgtcccg ggagctgcgg atcgcagccc
     5521 agcggcgtcc acacggcgaa cggcgatatg gctgccgatg cgatggtgtc gtggctggcg
     5581 cacgcgctca acgccgtggt caccaatccg gaaaacgatg ggtggttcga ccggtatggc
     5641 ctggaaaact cggaaaagtg cgaagggacg tacggcacga cctatcccgt cacgaatctc
     5701 tggggcgagt ccgcactggc caacgtc
//
LOCUS       DATANS010000235         2528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_96253, whole genome shotgun sequence.
ACCESSION   DATANS010000235 DATANS010000000
VERSION     DATANS010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96253"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..836)
                     /locus_tag="VHT22_03325"
                     /pseudo
     CDS             complement(1..836)
                     /locus_tag="VHT22_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(927..1913)
                     /gene="iolG"
                     /locus_tag="VHT22_03330"
     CDS             complement(927..1913)
                     /gene="iolG"
                     /locus_tag="VHT22_03330"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEX3136614.1"
                     /translation="MIEAALFGAGRIGKIHAGNLARQSGARLKYVADVNQAAAGELAS
                     QHGAKVATVDAALADKSVGAVVIASSTDTHADLLLRAAQAGKAIFCEKPVDLDLARAV
                     SAAEAVKRAGVTCLIGFQRRYDPTFAALKSRIVAGEIGEPEMLIVTSRDPGAPPVDYI
                     KVSGGIFKDMLIHDFDIFRWILDDEAATVYATGSCLTDPAIEAAGDIDSTTVTIRTRR
                     GRLAQINTSRRAAYGYDQRFEVLGGKGMLQAGNHRPTEVAAHTAASVSRDKPEHFFLE
                     RYRVAYANEMAHFIDAVAGGKPVRTSIADGVKALELADAATRSWRERRIVEL"
     gene            complement(1957..>2528)
                     /gene="iolB"
                     /locus_tag="VHT22_03335"
     CDS             complement(1957..>2528)
                     /gene="iolB"
                     /locus_tag="VHT22_03335"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEX3136615.1"
                     /translation="VFDDRAPYAVYLPPGETARIVAQTAAEIGIGSAPAAAGPPARLI
                     EPGSMKRFKRGEGSNTRYVCDILPQTEPASHLLLVEVVTPAGHSSSYPPHKHDTDNLP
                     TESSLEETYYHRLKPSQGFAFQRVYTDDRSIDEALAVEDHDVVLVPRGYHPVVVPHGY
                     DSYYLNVMAGPKREWHFKNDPAHEWMLKR"
BASE COUNT          379 a          828 c          874 g          447 t
ORIGIN      
        1 ctcgtctgca gcgactcgcg cagcgcgagc gcgatgcgcg tcgcttcggt cgcatcgtgc
       61 aaggtcagcg atggcgtgcg atcgtcgagc accgcatcga cgaaatgcgc gatttcgtgc
      121 aggaatgccg cctcgaagcg ctcgtagaac gtcggcgtgc attcgttgcg aacgccatgc
      181 gcgtcggaaa tgtcgacccg gtcgagccgc gggttcgcac cgatggcgag ccgcccggcg
      241 gtgccgaaaa tctcggtcgc ggtctcgtga ccgtgcgcca tcgtgcgcga cgcgtagaag
      301 caggcgagct tgccgccgtc gaattcgcag gtggccacgc cgttgtcgac gtcgccgaat
      361 tcgcgcaagc cctggtgaat cgcgaccgtg cccgcggcga acacgcgcag cggccgcgga
      421 ttgcccagga accagcgcgc gaggtcgatg tcgtgcaccg tgcagtcgag aaaaatgccc
      481 ccgcttgtcg gcgcgaagcg gacgaagaat cccgacggat cgttcatgtc gaggttctgc
      541 gagcgcacca gaaacggcgc gcctatcatg cccgcctgga tcttgtcgta ggcatcgcga
      601 tagctgggat cgaagcggcg cccgtagccg atcatcacct tgagcttcgg ccgcttggcg
      661 gcttcccgct gcacgcgctc gcagtcggcg atctccagcg acagcggctt ttcgcagaac
      721 acgtgcttgc ccgcctgcag gccctcgatg atctgcgcgg cgtgcagggt cgtcggagtg
      781 accaggaaca ccgcgtcgac ctcgggatcg gccagcatcg ccgcgtagtc gccgtacccg
      841 cgcgtgacgc cgagttgttg ctcggcccag gcggccgagg cccgcgatcc cgatgcgcag
      901 gcggcgtttc gcgtcgaccg atgcggtcat agctcgacga tccggcgttc gcgccacgac
      961 cgcgtcgccg cgtcggccag ctccagcgcc ttgaccccgt cggcgatgct cgtgcgcacc
     1021 ggcttgccgc cggcgaccgc gtcgatgaaa tgcgccattt cgttggcgta cgcgacgcga
     1081 tagcgctcga ggaaaaaatg ctcgggcttg tcgcggctta cgcttgcggc ggtgtgcgca
     1141 gcgacttcgg tcgggcggtg gttgcccgcc tgcagcattc ccttgccgcc gagcacctcg
     1201 aaacgctggt cgtaaccgta ggccgcgcgg cggctggtgt tgatctgcgc gagccgcccg
     1261 cggcgggtgc ggatcgtcac cgtcgtcgaa tcgatgtcgc ccgccgcttc gatcgccgga
     1321 tcggtcaggc agctgccggt ggcgtagacg gtggcggctt cgtcgtcgag aatccagcgg
     1381 aagatatcga agtcgtggat cagcatgtcc ttgaatatgc cgccggagac cttgatgtag
     1441 tccaccggcg gcgcgccggg atcgcggctg gtgacgatca gcatttccgg ctcgccgatc
     1501 tcgccggcga cgatgcgcga cttcagcgcg gcgaacgtcg gatcgtagcg gcgctggaag
     1561 ccgatcaggc aggtgacgcc ggcgcgcttg acggcttccg cagcgctgac cgcgcgcgcc
     1621 agatcgagat cgaccggctt ttcgcaaaag atcgctttgc cggcttgcgc ggcgcgcagc
     1681 agcaggtcgg cgtgagtatc ggtgctcgag gcgatgacga cggccccgac cgacttgtcg
     1741 gccagcgccg cgtcgacggt cgcgaccttc gcgccatgct gcgacgcgag ctcgccggcc
     1801 gcggcctggt tgacgtcggc cacgtacttg agccgagcgc cggactgccg cgcgaggttg
     1861 ccggcgtgga tcttgccgat ccggccggca ccgaataatg ccgcttcaat cattgttcgc
     1921 tccgtgagcg cgggattgtc aatgccaagc gcggctttag cgcttgagca tccattcgtg
     1981 cgccgggtcg ttcttgaaat gccattcccg cttcgggccc gccatcacgt tgaggtagta
     2041 cgagtcgtac ccgtgcggca cgacgaccgg atggtagccg cgcggcacca gcacgacgtc
     2101 gtgatcctcg accgcgagcg cttcgtcgat cgagcggtcg tcggtataga cgcgttggaa
     2161 cgcgaatccc tgcgaaggct tcagccggtg atagtaggtc tcctccagcg agctttcagt
     2221 gggcaggttg tcggtgtcgt gcttgtgtgg cgggtagctc gacgagtgcc ccgccggcgt
     2281 gacgacttcg accagcagca ggtgcgaagc gggctcggtc tgcggcagga tgtcgcagac
     2341 atagcgcgtg ttgctgcctt cgccgcgctt gaagcgcttc atcgatcccg gttcgatcag
     2401 ccgtgccggc gggccggccg ccgcgggtgc gctgccgatg ccgatctctg ccgcggtttg
     2461 cgcaacgatg cgcgcggtct cgcccggcgg caggtagacc gcatacggcg cccggtcgtc
     2521 gaacacgc
//
LOCUS       DATANS010000236         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_683664, whole genome shotgun sequence.
ACCESSION   DATANS010000236 DATANS010000000
VERSION     DATANS010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683664"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VHT22_03340"
     CDS             <1..852
                     /locus_tag="VHT22_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX3136616.1"
                     /translation="DVVDADACRSACQGIDIVLHEAALGSVPRSIEDPLRTHAANATG
                     FLNMLVAARDAGCERFVYAASSSTYGDHPGLPKVEDAIGRPLSPYAVTKYLDELYAQV
                     FSRCYGVVATGLRYFNVFGPRQDAEGAYAAVIPRWAAAMLSGTPIAIYGDGETTRDFC
                     YVGNVVQANLLAATAPAATVSGEVYNVAVGGRISLNDLYAALRDLVAERHAGLRVEAP
                     IYGDFRAGDVRHSQADIGKAIGAFGYAPTHSLHAGLREALPWYEAHSRTASIARAERE
                     PGVRGTA"
     gene            833..>1577
                     /locus_tag="VHT22_03345"
     CDS             833..>1577
                     /locus_tag="VHT22_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX3136617.1"
                     /translation="MFEVRPEAGAAGGAAVRVHRLIALIALVLAGVAGAAGAPSAGDL
                     AAQALAYEHGEGVPKDQRKAAALYCEAARSGDVGAMFNLGWMYANGRGVERDDALAAY
                     FFTRAAREGHPQAREMLKFVDAGQVVFPQCMLMPPPDPASGDAVHTAQALDSLAYADL
                     PPDKRRIAEVVVKAASQYGIDARLALAIVSAESNFQPDARSDKDARGVMQLIAATATR
                     FNVRNRFNVEDNVQGGLSYLRWLLAYYQGR"
BASE COUNT          239 a          509 c          558 g          271 t
ORIGIN      
        1 gacgtcgtcg atgccgacgc ctgtcgcagc gcctgccagg gaatcgatat cgtcctgcac
       61 gaagcggcgc tcggttcggt gccgagatcg atcgaggatc cgctgcgcac gcacgccgcc
      121 aacgcgaccg gattcctcaa catgctggtg gcggcgcgcg atgcgggctg cgaacgcttc
      181 gtctatgcag cgtcgagctc gacatacggc gaccatccgg gtctgccgaa ggtcgaggat
      241 gcgatcggcc ggccgctatc gccttatgca gtgaccaagt acctggacga gctctatgcc
      301 caggtgttct ctcgctgtta cggcgtcgtc gcgaccggac tgcgctattt caatgtgttc
      361 ggtccgcgcc aggacgccga gggcgcgtac gcggcggtga ttccgcgctg ggcggcggcg
      421 atgctgtccg ggacgccgat cgcgatctac ggcgacggcg agacgacgcg cgacttctgc
      481 tatgtcggca atgtcgtgca ggccaatctg ctggcagcga ccgctcccgc ggcgacggtc
      541 tcgggagagg tctacaacgt cgcggtcggc gggcgcatat cgctcaacga tctgtacgcg
      601 gcgttgcgcg acctggtcgc cgagcggcat gccggcttgc gcgtcgaggc gccgatctac
      661 ggtgattttc gcgcgggtga tgtgcggcac tcgcaagccg atatcggcaa ggcgatcggg
      721 gcgttcggct acgcgccgac gcactcgctg catgcgggtc tgcgcgaagc gttgccctgg
      781 tacgaagcgc attcgcggac ggcttcgatc gcccgcgccg aaagggaacc cggtgttcga
      841 ggtacggcct gaagcgggcg ctgccggtgg agcggcggtc cgcgttcatc gcctgatcgc
      901 gctgatcgcg ctggtgctgg ccggcgtggc cggcgccgcc ggggcgcctt cggctggcga
      961 tctggccgcg caggcgctgg cgtacgagca tggcgaaggt gtgccgaagg accagcgcaa
     1021 ggcggcagcg ctttattgcg aagcggcgcg ctccggagac gtcggcgcga tgttcaacct
     1081 cggatggatg tacgccaacg ggcgcggcgt ggaacgtgac gatgcgctcg cggcctactt
     1141 tttcacgcgt gccgcccgcg aaggccaccc gcaggcgcgt gaaatgctca aattcgtcga
     1201 tgcgggccag gtcgtcttcc cacaatgcat gttgatgccg ccgccggacc cggcgtcggg
     1261 cgacgccgtc cacacggcgc aggctctcga ctccctggcc tacgccgatc tcccgccgga
     1321 caagcgcagg atcgccgagg tggtggtcaa ggccgcctcg caatacggca tcgacgcgag
     1381 acttgcgctg gcgatcgtct cggccgagtc gaacttccag ccggacgcgc gctcggacaa
     1441 ggatgcgcgc ggcgtgatgc agttgatcgc ggcgaccgcg acgcgattca acgttcgcaa
     1501 tcgcttcaac gtcgaggaca acgtgcaggg cgggctttcc tatcttcgct ggctgctcgc
     1561 ctactaccag ggtcggg
//
LOCUS       DATANS010000237         1899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_390434, whole genome shotgun sequence.
ACCESSION   DATANS010000237 DATANS010000000
VERSION     DATANS010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390434"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VHT22_03350"
     CDS             complement(<1..615)
                     /locus_tag="VHT22_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX3136618.1"
                     /translation="MNDGAGSGGRLAPQRGFARLFNPRAIAVVGASPDIKRIGGQPIP
                     ILLDCGYRGGIYPVNPKYPKIGDLACYASVADVPTPCDLALVAVAGRLVPEVIRQCGK
                     AGIAFATVFSAGFREVGPEGVALEAELKSAVAESGVRMIGPNCIGTMNLVNRVYCGFG
                     VGFKNPHLKNGPVAFVSQSGGFAYSVVALADAEGMGFNYIVSAGN"
     gene            complement(619..1572)
                     /locus_tag="VHT22_03355"
     CDS             complement(619..1572)
                     /locus_tag="VHT22_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX3136619.1"
                     /translation="MRIPVLIACIVSSAAVYAQNYPARTVRIVVPYSAGGNTDFTARV
                     IATKLTDVFGQQVVVENRAGGATNIGSDLVAKAAPDGYTILMGGASNAINMSLYQKLP
                     YDTLRDFAPISLCVKGANVLAVHPSLPVKTVKELIATAKTRPGKLNYASSGLGSSNQM
                     AGELFKLMAGVNIVHVPYKGNSPALTDTIGGQVEMIFSGVPLLVPHIEAGRIRAIGIG
                     SLKRFPALPSVPTIDESGLKGYEATTWFGLMAPVKVPKDIVTRLNVEVGKILASSDVN
                     SRFINEGVEPIGGTPEFFTAFIRDEIAKYAKVVKAANLKGE"
     gene            complement(1569..>1899)
                     /locus_tag="VHT22_03360"
     CDS             complement(1569..>1899)
                     /locus_tag="VHT22_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3136620.1"
                     /translation="ETRGMFNAALLARLPRGARVINISRGEIVDEPALIAALQGGQLA
                     GAYLDVFVQEPLPASSPLWDLPNVIVTPHNSVASAGSDARVNALFLDNLQRWADNRPL
                     INEVKKP"
BASE COUNT          333 a          580 c          624 g          362 t
ORIGIN      
        1 gttgcccgcg gacacgatgt agttgaaacc catgccttcg gcgtcggcga gcgcgactac
       61 ggagtatgcg aagccgccgc tttgcgacac gaaagcgacc gggccgtttt taaggtgcgg
      121 attcttgaag ccgacgccga agccgcagta cacgcggttg acgagattca tcgtgcctat
      181 gcagttggga ccgatcatgc gcacgccgct ctcggcgacg gcggacttca actcggcttc
      241 gagcgcgacg ccttccggcc cgacttcgcg gaaaccggcg ctgaatacgg tcgcgaacgc
      301 gatgccggcc ttgccacatt gacggatcac ctcgggtacg aggcggccgg cgaccgcgac
      361 gagcgcgaga tcgcacggcg taggaacgtc ggctacgctc gcgtagcacg cgagatcgcc
      421 gatcttgggg tacttgggat tgacggggta gatgccgccg cgatagccgc agtcgagcag
      481 tatgggtatc ggctggccgc cgatgcgctt gatgtcgggc gacgcgccga ccacggcgat
      541 cgcgcgcgga ttgaagagcc gcgcaaagcc acgttgcggt gccagtctgc cgccgctgcc
      601 cgcgccgtca ttcaccgctc attcgccttt gagattggcg gctttcacga ctttcgcgta
      661 tttcgcgatt tcgtcgcgga tgaaagccgt gaagaattcc ggcgtgccgc cgatcggctc
      721 gacgccctcg ttgataaagc gcgagttcac atcgctgctt gcaaggatct tgccgacctc
      781 gacgttgagg cgcgtgacga tgtctttggg cactttgacc ggggccatca ggccgaacca
      841 cgttgtcgcc tcataacctt tgagaccgga ttcatctatc gtaggcaccg acggcagtgc
      901 aggaaaacgc ttgaggctgc cgatgccgat cgcgcggatg cgcccggcct cgatgtgcgg
      961 caccagcagc ggcacgccgc tgaaaatcat ttcgacctgg ccgccgatgg tgtccgtcag
     1021 cgccggcgaa ttgcctttgt acggaacatg cacgatgttg acgccggcca tcaacttgaa
     1081 gagctcgccc gccatctgat tcgagctgcc gaggcccgac gacgcgtaat tgagcttgcc
     1141 gggccgtgtt ttcgcagtcg cgatcagctc tttcaccgtc ttcacgggca gcgacggatg
     1201 cacggcgagc acgttggcgc ccttcacgca caggctgatc ggcgcgaaat cgcgcagcgt
     1261 gtcgtacggc agcttctgat acaggctcat gttgatcgcg ttggacgcac cgcccatcaa
     1321 tatggtgtag ccatcgggtg ccgctttcgc gaccaggtcg gagccgatat tggtcgcgcc
     1381 gcctgcgcgg ttttcgacca cgacctgttg gccgaacacg tcggtcagct tggttgcgat
     1441 gacgcgcgcg gtgaaatccg tgttgccgcc cgccgaataa ggcacgacga tgcgcaccgt
     1501 gcgcgcgggg tagttttgcg cgtaaacagc ggcgctcgat acgatacagg cgataaggac
     1561 cgggattctc atggtttttt gacttcgttg ataagcggcc ggttgtctgc ccagcgctgg
     1621 aggttatcga ggaacaatgc gttgacacgg gcatcgctgc cggcggacgc caccgagttg
     1681 tgcggcgtga cgatgacgtt gggcaggtcc catagcggag aagacgccgg caacggttcc
     1741 tgcacgaaga cgtcgagata cgcgccggcg agctggccgc cctggagcgc ggcgatcagt
     1801 gccggctcgt cgacgatttc gccgcggctg atgttgatga cgcgcgcgcc tcgcggcagc
     1861 ctcgcgagca gcgcggcgtt gaacatgccg cgcgtctcg
//
LOCUS       DATANS010000238         2929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_727038, whole genome shotgun sequence.
ACCESSION   DATANS010000238 DATANS010000000
VERSION     DATANS010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727038"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /gene="dprA"
                     /locus_tag="VHT22_03365"
     CDS             <1..826
                     /gene="dprA"
                     /locus_tag="VHT22_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX3136621.1"
                     /translation="SALLTIGDPPPVLHYAGKPDLLNRPALAIVGSRNATPQGRENAE
                     AFAAALSAAGLTIVSGLAQGIDAAAHRGGLAGAGSSVAVVGTGIDRIYPAGNKALAHR
                     LAAEGGLLSQFTLGTPPLPGNFPRRNRVISGLVRGVLVVEATPNSGSLITARFAAEQG
                     REVFAIPGSIHSPFSKGCHRLIKDGAKLVETAQDVLEELGLAPARASDRAWAGTPAPD
                     GDAARVLAALGHDPADVDLLAERTGLDASAIAVALVELELAGQVAQLLGGAFQRVM"
     gene            953..>2929
                     /locus_tag="VHT22_03370"
     CDS             953..>2929
                     /locus_tag="VHT22_03370"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEX3136622.1"
                     /translation="MAKSLIIAEKPSVAADVARALGGFTRHDDYFESDRYLLSSAIGH
                     LLEIGMPEDEEVKRGKWTFAHLPAIPSQFALKPIEKSEARLRLLLKLLKRKDVSELVN
                     ACDAGREGELIFRNIAQYARNTKPIRRLWLQSMTQGAIRDAFGKLRSDEAMRPLADAA
                     VCRSESDWLVGINGTRAMTAFNSKSGGFQLTTVGRVQTPTLAILVEREEKIRAFVPRD
                     YWEVLGTFRAAGGAYGGRWFDEKFKKADDDPDRRAERLWDETRARAIAARCDGKPGTV
                     TEETKPSTQVAPLLYDLTSLQREANGRFGLSARTTLSLAQALYEKHKALTYPRTDSRA
                     LPEDYLPTVKATMEALRESNAYAVFAGQILKSKWVRPNKRIFDDSKISDHFAIIPTLQ
                     RPKNLSELETKLYDLVVKRFLAVFYPPAEYLLTTRITRVAGEPFKTEGKVLVSPGWLA
                     VYGKEAQGDDDATLAPVRQDEKVATEEIKVVASQTRPPPRFSEATLLSAMEGAGKLIE
                     DDELREAMREKGLGTPATRAQIIEGLIQEKYVLREGRELQPTAKAFSLMTLLHGLGVP
                     ELFSPELTAEWEFKLAQMEHGKLKREQFMREIVKITKHIVAQAKNFESDTIPGDFATL
                     SARCPKCGGEVHEKYKKFQCMNPSCDFSFWKIMGG"
BASE COUNT          535 a          954 c          977 g          463 t
ORIGIN      
        1 gagcgcgttg cttacgatcg gcgatccgcc gcccgtgctc cattacgcgg gcaagcccga
       61 cctgttgaac cgccccgcgc tggcgatcgt cggcagccgc aatgcgacgc cgcagggccg
      121 cgaaaatgcc gaggcgttcg ccgccgcgct gtcggcagcg ggactcacga ttgtcagcgg
      181 gttggcgcag ggaatcgatg cggccgccca ccgcggcggg cttgccggcg ccggcagcag
      241 cgtcgcggtt gtcgggaccg gaatcgaccg catctatccg gctggtaaca aggcgcttgc
      301 gcaccgcctg gccgcagaag gaggcctgct gtcgcagttc acgctcggca cgccgccgct
      361 gccgggaaat ttcccccgcc gcaatcgcgt gatcagcggc cttgtccggg gtgtcctggt
      421 cgtcgaggcg acgccgaaca gcggctcatt gatcacggcg cgctttgccg cagagcaggg
      481 gcgcgaagtg tttgcgattc ccggttcgat tcattcgccg ttctcgaaag ggtgtcatcg
      541 cctgatcaag gacggcgcca agctcgtcga gacggcgcag gatgtcctgg aagagctggg
      601 cttggcgccg gcgagggctt ccgaccgggc gtgggcgggc acgccggcgc ccgacggcga
      661 tgctgcgagg gtcctcgcgg cgctcggcca cgatccggcc gacgtcgacc tgcttgccga
      721 acgcaccggg ctcgacgcca gcgcgatcgc ggtcgccctg gtcgagctcg agctcgccgg
      781 ccaagtcgcg caactcctcg ggggagcgtt ccagcgcgtt atgtgataca taggcgtccc
      841 acgccggttt gagagccgca tcagcgccga ctgagcgccc actgagagca gtccacggcg
      901 gctttgctat tatctgccct cctttttttg gccccaagat cgtgaacccc tgatggcgaa
      961 gagcctgatc attgccgaaa agccttccgt cgccgccgac gtagcgcgcg cgctcggggg
     1021 gtttacgcgc cacgacgatt acttcgagag cgatcggtat ctgctgtcgt cggcgatcgg
     1081 tcacctgctc gagatcggca tgcccgagga cgaggaggtc aagcggggca agtggacctt
     1141 cgcgcatctg ccggcgatcc cgtcgcaatt tgcgctgaag ccgatcgaaa agtcggaagc
     1201 ccggttgcgg ctgctgctca agctcctcaa gcgcaaggac gtaagcgagc tggtcaacgc
     1261 ctgcgatgcc gggcgcgagg gtgagctgat ctttcgcaat atcgcccagt acgccaggaa
     1321 caccaagccg atccggcgcc tgtggctgca gtcgatgacc cagggcgcta tccgcgacgc
     1381 gttcgggaag ctccgcagcg atgaggcgat gcggccgctc gccgatgcag ccgtatgccg
     1441 gtcggaatcg gactggctgg tcggcatcaa cggcacgcgc gcgatgaccg cgttcaactc
     1501 caaatcgggc ggcttccagc tgaccacggt cggcagggtg cagacgccga ccctcgcgat
     1561 cctggtcgag cgcgaggaga agatccgcgc cttcgtcccc cgcgactatt gggaagtgct
     1621 cggcacgttc cgcgccgccg gcggcgccta cggcggccgc tggttcgacg aaaaattcaa
     1681 gaaagccgac gacgacccgg accggcgggc cgaacgcctc tgggacgaga cccgggcgcg
     1741 cgcgatcgcc gcgcgctgcg atggcaagcc gggcacggtc accgaggaga ccaagccttc
     1801 gacccaggtg gcgccgcttc tctacgacct gaccagcctg cagcgcgaag ccaacggccg
     1861 attcggtctg tcggcgcgca cgacgctgtc gctcgcgcag gcgctctacg aaaagcacaa
     1921 ggcactgact tacccgcgga ccgattcgcg cgcgctgccc gaggattacc tgcccaccgt
     1981 caaggcgacg atggaagcgc tgcgcgagtc caacgcctac gcggtgttcg ccggccagat
     2041 cctcaagtcg aaatgggtga ggcccaacaa gcggatcttc gacgacagca agatctccga
     2101 tcacttcgcg atcatcccga ccttgcagcg tccgaagaac ctttccgagc tcgagaccaa
     2161 gctttacgac ctggtcgtca agcgattcct cgcggtgttc tatccgcccg ccgagtatct
     2221 gctgacgacg cggatcaccc gggtcgccgg cgagccgttc aagaccgagg gcaaggtgct
     2281 ggtctcgccg ggctggctcg cggtctacgg caaggaagct caaggcgacg acgatgcgac
     2341 gctcgcgccg gtccgccagg atgaaaaagt ggcgaccgag gaaatcaagg tggtcgcgag
     2401 ccaaacgcgg ccgccgccac gcttctccga ggcgacgcta ctgtcggcaa tggaaggcgc
     2461 cggcaaactg atcgaggacg acgagctgcg cgaagcgatg cgtgaaaagg gccttggcac
     2521 gccggcgacc cgcgcccaga tcatcgaagg cctgatccag gaaaagtatg tgctgcgcga
     2581 gggtcgcgag ctgcagccga ccgcgaaggc gttctcgctg atgacgctgt tgcacggact
     2641 gggcgtgccg gagttgttct cacccgagct caccgcggag tgggagttca agctggcgca
     2701 gatggagcac ggcaagctca agcgcgagca attcatgcgc gagatcgtca agatcaccaa
     2761 gcatatcgtc gcgcaagcga agaacttcga aagcgacacc ataccgggcg atttcgcgac
     2821 gctgtcagcg cgctgtccca aatgcggcgg cgaggtgcac gagaagtaca agaagttcca
     2881 gtgcatgaac ccctcgtgcg acttctcgtt ctggaagatc atgggcggc
//
LOCUS       DATANS010000239         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_643333, whole genome shotgun sequence.
ACCESSION   DATANS010000239 DATANS010000000
VERSION     DATANS010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643333"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..>1713
                     /gene="phaC"
                     /locus_tag="VHT22_03375"
     CDS             120..>1713
                     /gene="phaC"
                     /locus_tag="VHT22_03375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEX3136623.1"
                     /translation="MPVSLPSPDMASRLPAVSIDPAAAAQLTERYARRFEALWTRATE
                     GSTVPMPAPGPADRRFSDKAWREHPYFAWLRDAYLLYGEYVRELAALAQTDDAMKRRL
                     VFLAQQYVDAISPSNFLATNPEALKRALETGGTSIAQGLSNLVSDAQRGRIAMTDESA
                     FEVGRNLATTPGSVVYRNPLIELIQYAPTTSKVAKRPLLIVPPCINKYYILDLQPENS
                     FVRYAVGEGHTVFMVSWRNIPSELGGLTWDDYLEQGVLAALDAACEIAGSKTVNALGF
                     CVGGTLLACALAVLAARRETPVASTTLLATMLDFADPGDIGVYVSREGLAARMPALCG
                     GGRIQGSELASAFASLRPNELVWNYVVGNYLKGQTPPAFDLLYWNSDSANLPGPMYVE
                     YLRDMYLDNRLREPGALSMCGQPIDLKRITMPAYILATREDHIVPWRTAYKSTSLLGG
                     DLTFVLGASGHIAGVINPPAKNRRNFWTNELLTDDPQDWLAGARTQTGSWWPHWAAWL
                     NDYTGGLRAAPKSPGSDKHRPLS"
BASE COUNT          312 a          576 c          539 g          286 t
ORIGIN      
        1 caactcagtt gccaactgac gcggcaaacg aatggtcgga gctcgcgcac aggtggcagg
       61 acgtcggcgc gcaatggacg cagctgtggg ccggcgccgc gctggggacg atgcggtcga
      121 tgccggtcag cttgccgtca ccggacatgg cttcccggct gccggcagta tcgatcgatc
      181 cggcggcggc agcgcaactg accgaacgct acgccaggag attcgaggca ctctggacgc
      241 gcgcgacgga aggcagcacc gtgccgatgc cggcacccgg ccccgcggac cggcgcttct
      301 cggacaaggc gtggcgcgag catccgtact tcgcgtggtt gcgcgacgcc tacttgctct
      361 acggcgagta tgtgcgcgag ctcgctgcgt tggcccaaac cgacgatgcc atgaagcggc
      421 gactggtctt tctcgcccag cagtatgtgg acgcgatttc accgtcgaac ttcctcgcca
      481 ccaatccgga ggcgctgaaa cgcgcgctcg agaccggcgg tacgtcgatt gcgcaaggcc
      541 tgtcaaacct ggtctcggat gcgcagcgcg ggcgcatcgc gatgaccgac gaaagcgcat
      601 tcgaggtcgg ccgcaacctc gcgacgactc cgggcagcgt cgtatatcgc aatccgttga
      661 tcgagctcat ccagtacgcg ccgacgacct ccaaggtcgc caagcggccg ctgctgatcg
      721 tgccgccttg catcaacaag tactacatcc tcgacctgca gccggaaaat tctttcgtgc
      781 gctacgcggt cggcgaaggc cacacggtct tcatggtctc atggcgcaac attccatccg
      841 agctcggcgg tctcacctgg gacgactatc tcgagcaagg cgtcctggcc gcgctcgatg
      901 cggcgtgcga gatcgccggc agcaagacgg tcaatgcgct cggattctgc gtcggcggga
      961 cactgcttgc ctgcgcgctg gccgtccttg cggcgcgccg ggagacgccg gtcgccagca
     1021 cgaccctcct tgcaacgatg ctcgacttcg ccgatccggg cgacatcggc gtctatgtct
     1081 cacgcgaagg gcttgcagcg cgcatgccgg cgctttgcgg cggcggccgc atccagggca
     1141 gcgagctggc cagcgcattc gcaagcctcc gacccaacga gctcgtctgg aactacgtcg
     1201 tcggaaatta tctgaaagga cagacgccac ccgcgttcga cctgctctac tggaacagcg
     1261 actcggccaa tcttccgggt ccgatgtacg tcgagtacct gcgcgacatg tatctggaca
     1321 atcgattgcg cgagcctgga gcgctgtcga tgtgcggtca accgatcgac ctcaagcgca
     1381 tcacgatgcc tgcctatatt ctcgccaccc gcgaagacca tatcgtcccc tggcgcaccg
     1441 catacaaaag cacgtccctg ctcgggggcg acctgacctt cgtgctcggc gcctcgggcc
     1501 atatcgccgg cgtgatcaat ccaccggcga aaaaccgacg caacttctgg accaatgaac
     1561 tgctgaccga tgatccgcaa gactggctgg cgggcgcccg gacgcagacg ggcagctggt
     1621 ggccacactg ggcggcatgg ctcaacgatt acaccggcgg gcttcgggcg gcgcccaaat
     1681 cgccgggcag tgacaagcat cggccgctgt cgc
//
LOCUS       DATANS010000240         1511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_181398, whole genome shotgun sequence.
ACCESSION   DATANS010000240 DATANS010000000
VERSION     DATANS010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181398"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1511
                     /gene="htpG"
                     /locus_tag="VHT22_03380"
     CDS             <1..>1511
                     /gene="htpG"
                     /locus_tag="VHT22_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEX3136624.1"
                     /translation="QGDQKKDAQLIGQFGVGFYSSFIVADRVDVYSRRAGLDAAAGVH
                     WQSAGDGEFSVATVELPERGTRIVLHLKEGEQDFADAYRIRSLVRKYSDHIAFPVRMH
                     VEKEEKDKDKDKDAAPEYETVNHAQALWTRPRTEVQDEEYKEFYRHITHDFTDPLAWS
                     HGKVEGTKDYTSLLYVPSRAPFDLWHREAARGLKLYVRRVFIMDDAEQFLPLYLRFVK
                     GVVDSADLPLNISREMLQQDAAIDSMRTGLARRVLDLLTKMAKDEPEKYAAFWKEFGT
                     VLKEGPAEDHANRERIAKLLRFSSTHADKAEQDVSLEDYIARMKPQQKEIYFVIADSF
                     AAAKSSPHLEILRGKGIEVLLLSDRVDEWLVDHLREFEGKQLRNVARGELDIDAIQSD
                     AEKQQRETLSKEHAALVERMKKSLQDRVSDVRVTNRLADSPAVLVLGAHDLGAQMRRI
                     LEAAGQKVPTSKPALEINVQHPLLQRIESTGEDTAFDDLSMLLFEQATLADGG"
BASE COUNT          292 a          460 c          509 g          250 t
ORIGIN      
        1 gcagggcgac cagaagaagg acgcgcagct gatcggccag ttcggcgtcg gcttctattc
       61 ttcgttcatc gttgcggacc gcgtcgacgt ctattcgcgc cgcgccggcc tcgatgcggc
      121 ggcgggcgtg cattggcagt cggccggcga cggcgagttc agcgtcgcga ccgtcgagct
      181 gcccgagcgc ggcacgcgca tcgtgctgca tctcaaggaa ggcgagcagg atttcgccga
      241 cgcgtatcgc atccgctcgc tggtgcgaaa gtattcggac cacatcgcct tcccggtccg
      301 catgcacgtc gagaaggagg agaaggataa ggacaaggac aaggacgccg cgccggagta
      361 cgaaaccgtc aatcacgcgc aggcgctgtg gacgcggccg cgcacggaag tgcaggacga
      421 ggaatacaag gagttctatc gccacatcac gcacgacttc accgatccgc tggcgtggtc
      481 gcacggcaag gtcgagggca cgaaggacta caccagcctg ctctatgtgc cgtcgcgggc
      541 gccgttcgat ctctggcatc gcgaagcggc gcgcggtctc aagctgtacg tgcgtcgcgt
      601 cttcatcatg gacgacgccg agcagttcct gccgttgtac ctgcgcttcg tgaagggcgt
      661 ggtcgattcg gccgatctgc cgctgaacat ctctcgcgaa atgctgcaac aggatgcggc
      721 catcgacagc atgcgcaccg gcctcgcgcg tcgcgtgctc gacctgctga cgaaaatggc
      781 gaaggacgag ccggagaagt acgcggcgtt ctggaaggag ttcggtacgg tgctgaagga
      841 aggtccggcc gaagatcacg ccaatcgcga gcgcatcgcc aagctgctgc ggttctccag
      901 cacgcatgcc gacaaggcgg agcaggacgt gtcgctcgag gattacatcg cgcgcatgaa
      961 gccgcagcag aaggaaatct acttcgtgat cgccgacagc ttcgcggccg cgaagtccag
     1021 tccgcacctg gaaatcctgc gtggcaaggg catcgaggtg ctgttgctgt cggatcgcgt
     1081 cgacgagtgg ctggtcgatc atctgcgcga gttcgagggc aaacagctgc gcaacgtcgc
     1141 tcgcggcgag ctcgacatcg acgccatcca gtccgacgca gagaagcagc agcgcgagac
     1201 gctgtccaaa gagcatgctg ctctggtcga acgcatgaag aagtcgctgc aggatcgcgt
     1261 cagcgacgtg cgcgtcacca atcgcctggc cgattcgccg gcggtcctgg tgctcggtgc
     1321 gcacgatctg ggcgcgcaga tgcgtcgcat cctcgaagcg gcgggccaga aggtgccgac
     1381 cagcaagccg gcgctggaga tcaatgtcca gcatccgctg ctgcagcgga tcgagtcgac
     1441 cggcgaagac actgcgttcg acgatctgtc gatgctgctg ttcgagcagg cgacgcttgc
     1501 cgatggcggt c
//
LOCUS       DATANS010000241         3033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_221965, whole genome shotgun sequence.
ACCESSION   DATANS010000241 DATANS010000000
VERSION     DATANS010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221965"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /gene="trpC"
                     /locus_tag="VHT22_03385"
     CDS             <1..617
                     /gene="trpC"
                     /locus_tag="VHT22_03385"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX3136625.1"
                     /translation="EIKRASPSRGILRDPFDPSAIAASYERAGATCLSVLTDRPFFQG
                     APEYLVDARNACALPALRKEFIIDEYQVAESRALGADAILLIVAALEDARLATLEACA
                     LDYGMDVLVEVHDRAELERALKLKTSLVGINNRNLRSFAVSLRTTIDLLPSVPVGKLV
                     VTESGILTQGDVASLRRHGVGAFLVGEAFMRSPDPGDALQALFG"
     gene            748..1842
                     /locus_tag="VHT22_03390"
     CDS             748..1842
                     /locus_tag="VHT22_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3136626.1"
                     /translation="MSAIVVENVSKHWTTAAGMVRAVEGLSFSLDAGTLNVLLGPSGC
                     GKSTTLRLIAGLDQADNGRISIDGRDVTRLPPAQRSISMVFQNYALFPHLSVAENIVF
                     GLRVRRVGPAERDRRLQRVAGLLGLTTLLDRRPSQLSGGQQQRVALGRAFIAETPVCL
                     MDEPLSNLDAQLRQEMRQEIRNLQRTLGITMVYVTHDQVEAMTMADRIILLSGGRIEQ
                     NGTPADLYESPENAFVARFIGTPPMNLLRLEHGAAGAVIAGTAGPPVATDDLAGATLG
                     VRPEHIVLGREDGVAALVDSVEYLGADSLLACHVGDQPLTVRVSGRTGLQHGDKVRLG
                     WASAAAHFFDGATQMRREVEWNHQPATMVA"
     gene            1881..>3033
                     /locus_tag="VHT22_03395"
     CDS             1881..>3033
                     /locus_tag="VHT22_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3136627.1"
                     /translation="MKYVTISRWIVALAAAALSSLAVAQAPVDISFYYPVAVGGPITK
                     IIDKFAVDFERENPGIKVHPIYSGTYQDSITKALTAVKSNDAPAMSVLLSTDMYTLID
                     EDAIVPWDPLIKTAEDRAWLQGFYPAFMENSQTGGKTWGIPFQRSTIVLYYNKEMFKD
                     AGLDPNHAPETWKEMAEYAQKLTKRDASGKVTQWGVQIPSSGFPYWLFQGLTTENGVQ
                     LMNAAGTETYYDKPAVIEAVQYWVDLTSKYKAHPEGIVEWGTTPKDFFERKVAMMWTT
                     TGNLTNVKANAKFPFGVAMLPAHKRRGSPTGGGNFYISKKASPAEQAAAFQFIKWITT
                     PERAAQWSIDTGYVAVRPDSYETDAMKKYVADFPQAAVARDQLKVSVAEL"
BASE COUNT          549 a          966 c         1006 g          512 t
ORIGIN      
        1 cggaaatcaa gcgggcgagc ccgagccgcg gcatcttgcg cgatccgttc gatccctccg
       61 ccatcgccgc atcgtacgag cgggcggggg cgacctgcct ctcggtgctg accgaccgac
      121 cttttttcca gggcgctccc gaatatctgg tcgacgcgcg caatgcatgc gcattgccgg
      181 cgcttcgcaa ggaattcatc atcgacgaat accaggtcgc ggagtcgcgc gcgctgggcg
      241 ccgatgcgat cctcctgatc gtcgccgcgc tggaagacgc ccgccttgcg acgctcgagg
      301 cgtgcgcgct cgactatggc atggacgtgc tggtcgaggt gcacgaccga gccgagctgg
      361 agcgcgccct caagctcaag acttcgctgg tcggcatcaa caatcggaac ctgcgcagct
      421 tcgccgtatc gctgcggacc acgatcgatc tgctgccaag cgttcccgtg ggcaagctgg
      481 tggtcaccga aagcggcatt ctgacgcagg gcgacgttgc gtcgctgcgc cgtcatgggg
      541 tgggcgcctt cctggtaggc gaggccttca tgcggtcacc cgacccgggt gacgcgctgc
      601 aagccctgtt cggctgaatc gacccgaaac gcagcgccgg ccgggcgatg ccgcgagcgc
      661 cggacgcgag ctgtttgcta tgatggcggg ccggaaggcg catgcaacgc cgccaaagcg
      721 cgaggtctgc cggtgccagg gggagcattg tcggcaatcg tcgtcgagaa cgtcagcaag
      781 cattggacga ccgcagccgg aatggtgagg gcggtcgagg gactttcttt ttcgctcgac
      841 gccggcacgc tcaacgttct gctcggtccg tccggctgcg gcaagtcgac gaccttgcgg
      901 ctgatcgctg gcctcgatca ggccgacaac ggccgcatct cgatcgacgg gcgcgacgtc
      961 actcggctcc cacccgcgca gcgcagcatt tcgatggtct tccagaatta cgcgctattc
     1021 ccgcacttga gcgtcgccga aaacatcgtg ttcggtctcc gcgtgcgtcg cgtcggtccc
     1081 gcggagcgcg accggcgcct gcagcgggtc gcaggactcc tcggcctgac tacgctgctc
     1141 gatcgtcggc catcgcaact ctcgggtggc cagcagcagc gggtggcgct gggccgcgcc
     1201 ttcatcgccg agacaccggt ctgcctgatg gacgagccgc tgtccaatct cgatgcgcag
     1261 ctgcggcagg agatgcggca ggagatacgc aacctgcagc gcacgctggg catcacgatg
     1321 gtctatgtga cgcacgacca ggtcgaggcg atgaccatgg cggatcggat catcctgctt
     1381 tccggcggtc gcatcgagca gaacggcaca ccggccgatc tctacgaaag ccccgagaac
     1441 gccttcgtcg cgcgcttcat cggaaccccg ccgatgaatc tgctgcggct cgaacacggc
     1501 gcggcgggcg cggtcatcgc cggcacggcg ggaccgccgg tggcgacgga cgacctggcc
     1561 ggcgcgacct tgggcgtgcg ccccgagcac atcgttctgg gccgcgagga cggcgtcgct
     1621 gcattggtcg actcggtcga gtacctgggc gccgattcgc tgctcgcatg ccacgtcggc
     1681 gaccagccgc tgaccgtgcg agtgagcgga cgcactggtc tgcagcatgg cgataaggtg
     1741 cggctcggct gggcgagcgc cgccgcgcat tttttcgacg gtgcgacgca gatgcggcgc
     1801 gaggtcgagt ggaatcatca acccgcaacc atggtcgctt aggccctggg cgtccaacgc
     1861 agtcaagagg aggagattcg atgaagtacg tcaccatttc gcgctggatc gtggcgctcg
     1921 ccgccgccgc gctgtcgagt ctggccgtcg cgcaggcgcc ggtcgacatt tcgttctact
     1981 acccggttgc cgtcggcggg ccgatcacca agatcatcga caagttcgcc gtcgatttcg
     2041 agcgtgaaaa tccggggatc aaggttcatc cgatctattc gggcacctat caggattcga
     2101 tcaccaaggc gctcaccgcc gtcaagagca acgacgctcc ggcgatgtcg gtcttgctgt
     2161 caacggatat gtacacgctg atcgacgagg atgcgatcgt gccgtgggac ccgctgatca
     2221 agacggcgga ggaccgcgcg tggctgcagg gcttttatcc ggcgttcatg gaaaacagcc
     2281 agaccggcgg caagacctgg ggcattccgt tccagcgctc gacgatcgtg ctctactaca
     2341 acaaggagat gttcaaggac gccggactcg atcccaacca tgcacccgag acgtggaagg
     2401 aaatggccga gtacgcgcag aagctgacca agcgtgacgc atcggggaag gtcacgcagt
     2461 ggggcgtgca gattccttcg tcggggtttc cctactggct gttccaggga ctgacgaccg
     2521 aaaacggcgt gcagctgatg aacgccgccg gaaccgagac ctactatgac aagccggccg
     2581 tcatcgaggc ggttcaatac tgggtcgatc ttacctccaa gtacaaggca catccggaag
     2641 gcatcgtcga atggggaacg acgccaaagg actttttcga gcgcaaggtc gcgatgatgt
     2701 ggaccaccac gggcaacctg acgaatgtga aggccaacgc caaattcccg ttcggcgtcg
     2761 cgatgctgcc ggcgcacaag cggcgcggct cgccgaccgg cggcggcaat ttctacatct
     2821 cgaagaaggc gagcccggcc gagcaggcgg cggcgttcca gttcatcaag tggatcacga
     2881 cgccggagcg cgccgcgcag tggtcgatcg acacgggtta cgtcgcggta cggccggatt
     2941 cctacgagac cgacgcgatg aagaaatacg tggccgactt cccgcaggcg gcggtcgcgc
     3001 gggaccagct caaggtctcg gtggccgaac tct
//
LOCUS       DATANS010000242         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_181402, whole genome shotgun sequence.
ACCESSION   DATANS010000242 DATANS010000000
VERSION     DATANS010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181402"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VHT22_03400"
     CDS             complement(<1..765)
                     /locus_tag="VHT22_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136628.1"
                     /translation="MRTLWSAALCGALACADAQAVVTRIVIDNIDAPTFDARDFGAVG
                     RYEKLSGKAFGEIDPADPLNAGITYIQDAPRNERGRVEYVIDISILKPIESSRGNGTL
                     LYDVVNRGARRAFEVFHVGAAGGNNPSRLADIGDAFLMRQGYTLVVSGWQADLPKNQG
                     DFTAYFPSARRGGGPLGKTITVELLVTKPAFTLSAGWDNGRALLAYPPFERQMDRARL
                     IRRAHADAPDEVSPRDEWSFGSCPDGKTVTPSNVDVC"
     gene            complement(812..1174)
                     /locus_tag="VHT22_03405"
     CDS             complement(812..1174)
                     /locus_tag="VHT22_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136629.1"
                     /translation="MLGQALGLTDAEFAALQADDYRASTLFNEREKAVMAWAEAMTLN
                     TAKRDDKVWNELRRLFSDAEIVEVSLITGMFNMINRLNDSFRTELEPEEYNRRQHGAV
                     GVTREALKDYACRLCAAD"
     gene            complement(1190..1444)
                     /locus_tag="VHT22_03410"
     CDS             complement(1190..1444)
                     /locus_tag="VHT22_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136630.1"
                     /translation="MAQRIAGIADADAAGAVKELFDSSNQLLGRTANLMRILAHSPHL
                     AKWVLPFIAAVRQPAAGAVSAVRLRNLAVLKTSTLNGCRY"
BASE COUNT          279 a          530 c          494 g          298 t
ORIGIN      
        1 gcagacgtcg acattgctcg gcgtcaccgt tttgccgtcg gggcagctgc cgaacgacca
       61 ctcgtcacgc ggactgactt cgtcgggcgc atcggcgtgc gcgcgccgga tcagccgcgc
      121 gcggtccatc tggcgctcaa acggtggata ggccagcagc gcgcggccgt tgtcccagcc
      181 ggcggacagt gtgaaggctg gcttggtcac caggagctcg acggtaattg tcttgccgag
      241 cgggccgccg ccgcgccgcg cggacgggaa atacgcggtg aaatcaccct ggttcttcgg
      301 caggtcagcc tgccagccgc tcacgaccag cgtatacccc tggcgcatca ggaacgcatc
      361 gccgatatcg gcgagccgcg acggattgtt gccgcccgcg gcgccgacgt gaaacacctc
      421 gaacgcgcgc cgcgcgccgc gattcaccac gtcgtagagc agcgtgccgt tgccgcgtga
      481 cgactcgatg ggtttcaata tgctgatatc gatcacgtac tcgacgcggc cgcgttcgtt
      541 gcgcggcgcg tcctggatgt acgtgatacc agcgttcagc gggtcggcag ggtcgatctc
      601 gccgaatgcc ttgcccgaca atttttcgta gcggccgacc gccccgaaat cgcgcgcatc
      661 gaaagtcggc gcgtcgatgt tgtcaatcac gatgcgcgta accaccgcct gcgcatcggc
      721 gcacgccaac gccccgcaca gcgctgcgct ccaaagggtt ctcatgcgat ccagtatagt
      781 ttttggcgcg cgcagcggca attgcaacca attaatccgc tgcgcacaac cggcacgcgt
      841 aatctttcag cgcctcgcgc gtcacgccca ccgcgccatg ctggcgccgg ttatattcct
      901 cgggctcaag ctcggtgcga aacgagtcgt tgagccggtt gatcatgttg aacatgccgg
      961 tgatcagcga cacttcgacg atctcggcat cgctgaacag gcggcgcagc tcgttccaca
     1021 ccttatcgtc gcgcttggcg gtgttcagcg tcatcgcttc ggcccacgcc atcaccgctt
     1081 tctcgcgctc gttgaagagc gtgctcgcgc ggtaatcgtc ggcctgcagc gccgcgaatt
     1141 cggcgtcggt gagtcccagt gcttgaccaa gcacccggtt gtgttccgtt caataccggc
     1201 agccgttgag cgtcgacgtc ttcagcacgg caagattcct gagccgcacg gcggacaccg
     1261 cgcccgccgc gggctggcgc accgccgcga taaacggcag cacccatttg gcaagatgcg
     1321 ggctgtgcgc aaggatgcgc atgaggttcg ccgtgcgtcc gagcagctgg ttggaagaat
     1381 cgaaaagctc tttgaccgcg ccggccgcgt cggcgtcggc gatccccgcg atgcgctggg
     1441 ccatggttac tttcccctta ttgaccactc gatacgagcg agccccatag taatccacgg
     1501 caccggcacg cgcgagttgt cgccgccacg caatttagtg cactatcacg cgtcgcaatc
     1561 agcgagctac ggaggaatcg atgttcatgc gcttgagtca a
//
LOCUS       DATANS010000243         2757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_598714, whole genome shotgun sequence.
ACCESSION   DATANS010000243 DATANS010000000
VERSION     DATANS010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598714"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VHT22_03415"
     CDS             <1..199
                     /locus_tag="VHT22_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3136631.1"
                     /translation="PLRFVADDDVVVIEARGEMTSRTGVPYNNEYCLIYRLRDGKIVE
                     ITEYNDSVLCERVLGPFSASE"
     gene            complement(183..680)
                     /locus_tag="VHT22_03420"
     CDS             complement(183..680)
                     /locus_tag="VHT22_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3136632.1"
                     /translation="MTLRFFAALVAACGLVTLQAARADAPQAPTADTAPAVQAELAYT
                     SYYVLDQKRALDFYVGLLGMVERQRITPSPGVTEIVLGFNKEPTSPGILLMHKADRTT
                     PYQIGDGFSRTIIHVSDIKAIVERLTKAGVTIVRQPTEVANLKLTYAMVRDPDGWLIE
                     FIQTH"
     gene            884..1267
                     /locus_tag="VHT22_03425"
     CDS             884..1267
                     /locus_tag="VHT22_03425"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX3136633.1"
                     /translation="MKRQVIKIPDAPSSPLYSQAIKVGPTIYVTGVPGIDPKTNKVAG
                     PTIQDQTRQALVNCENILRAAGAKLTDVAEVQVLLARPEDFAGLNEAYAQFFPKDPPV
                     RSVARLGPDLGSILVSIKLIAVVPE"
     gene            complement(1275..2207)
                     /locus_tag="VHT22_03430"
     CDS             complement(1275..2207)
                     /locus_tag="VHT22_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136634.1"
                     /translation="MRKRTLLLCALAYCSQAAAKKEDIEYVAEHLPEVSMDNRYATLP
                     LWTASATSRPDDGWTFAAQGSFSRATAGGLEVGGPMLSFAASRALSSRWTVTALGFAD
                     DLHLSGSDQRDLQTLFAPSTPLARPVASSFEGLDGSVNHYGAGLAFSLSSDQGWLGVH
                     RWVGGLLYEDVNLRDYRWNFTVLEGPDAGTRGQLDFDNDYRHFTPFAGLEVVRERGDW
                     TFSPHALLAWPLPRRGFVGHIVTDEFDIHGDQADAGHGKHFGDPSLTLGFDITYRPAH
                     LSVDVGTALTQALIEPIVHKGIDQNWLLSVRWQH"
     gene            complement(2248..>2757)
                     /locus_tag="VHT22_03435"
     CDS             complement(2248..>2757)
                     /locus_tag="VHT22_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX3136635.1"
                     /translation="QLERLSGQYATREQFVTELTLDPPNVSGDLAGDPLLDEDYLILS
                     TVHSAKGQEWDSVYVLNVSDGNFPNEHAAGRPDLIEEERRLLYVAMTRAKRDLHLIAP
                     LKYYITQQSRTGDAHVYGARSRFMTDQLMAQFEQKAWPAARESALRALDTKANVRVDA
                     AAALRAMWE"
BASE COUNT          501 a          879 c          884 g          493 t
ORIGIN      
        1 cccgctgcga ttcgttgccg acgacgacgt agtcgtgatc gaagcgcgcg gcgaaatgac
       61 gtcgaggacc ggcgtgccct acaacaacga gtattgcctc atctatcggc tgcgcgacgg
      121 caagatcgtc gagatcaccg agtacaacga ctcggtgttg tgcgagcgcg tgctcggccc
      181 gttcagtgcg tctgaatgaa ctcgatcagc cagccgtccg gatcgcgcac catcgcatag
      241 gtcagcttca ggttcgccac ttcggtcggc tgacgcacga tcgtgacgcc agccttggtc
      301 agccgctcga cgatcgcctt gatatcggac acgtgaatga tcgtgcggct gaagccgtcg
      361 ccgatctggt atggcgtcgt gcggtcggcc ttgtgcatca gcaggatgcc gggagaggtc
      421 ggctccttgt tgaagccgag cacgatctcg gtcacgcccg ggctcggcgt aattcgctga
      481 cgctccacca tgccgaggag cccgacgtag aaatccagcg cgcgcttttg atcgagcacg
      541 tagtagctgg tgtacgccag ttccgcttgc actgccggtg cggtgtccgc cgtcggcgct
      601 tgcggcgcgt ccgcgcgtgc agcctgcaga gtcacgagtc cgcacgctgc gaccagcgca
      661 gcgaagaatc gaagcgtcat tgtcaaagtc ccccgccgcg agttgcggac tgcagagtgg
      721 tacttggatc ccggagaaat cccggcgttc ccccgggatt gcgcgcgtca gtcgaccgta
      781 gtctatagcg atgaaacgtt taatggcatt caccgccgcc gcgctctgct gtgcgttgtg
      841 gttcgcggca ccggcgggca gcgaacctgc gaaggccccc tccatgaaac gacaagtcat
      901 caagattccc gacgccccga gctcgccgct gtacagccag gcgatcaaag tgggcccgac
      961 catttacgtc accggcgtgc ccggcatcga tccgaagacg aacaaagtcg ccggtccgac
     1021 gatccaggat cagacgcggc aggcgttggt gaactgcgag aacatcctgc gcgccgcggg
     1081 cgccaagctg accgatgtcg ccgaagtgca ggtgttactg gcgcggccgg aagatttcgc
     1141 gggcttgaac gaggcctatg cgcagttctt tccgaaagat ccgccagtgc gctccgtcgc
     1201 gcggctgggt ccggaccttg gttcgattct cgtctcgatc aaactgatcg cagtcgttcc
     1261 ggaatagcgc gcgctcagtg ctgccagcgc acgctgagca gccagttctg gtcgatgcct
     1321 ttgtggacga tgggctcgat cagggcctgg gtcagcgccg tgcccacatc gacgctcagg
     1381 tgcgccggtc gataggtgat atcgaagccg agcgtcagcg acgggtcgcc gaagtgcttg
     1441 ccgtggccgg cgtcggcctg gtcgccgtgg atgtcgaact catccgtaac gatgtgcccg
     1501 acgaagccgc gacgcggcag cggccatgcg agcagcgcgt gcggcgagaa cgtccagtcg
     1561 ccgcgctcgc gcacgacctc caaaccggcg aacggcgtga agtggcgata gtcgttgtcg
     1621 aagtcgagct gcccgcgcgt gcccgcgtcc gggccttcga ggaccgtgaa gttccagcgg
     1681 taatcgcgca ggttgacgtc ctcgtagagc aagccgccga cccagcgatg cacgccgagc
     1741 cagccttggt ccgaggacaa ggagaacgcg agtcctgcgc cgtagtgatt aacgctgccg
     1801 tcgagccctt cgaagctcga cgcgacggga cgtgcaagcg gtgtcgacgg cgcaaacaag
     1861 gtctgcaagt cgcgctggtc gctgcctgac aaatgcagat catccgcaaa gcccaatgca
     1921 gtgacggtcc agcgcgaaga cagcgcgcgc gatgcggcga acgacagcat cggcccgccg
     1981 acttcgagac ccccggcggt tgcccgactg aacgagcctt gcgcggcaaa cgtccagccg
     2041 tcatccggcc gcgaggtcgc cgacgcggtc cacaagggca acgtcgcgta gcggttgtcc
     2101 atcgagactt ccggcagatg ctcggcgacg tattcgatgt cttctttttt agcggcggcc
     2161 tgggaacagt aggccagcgc gcacaggagg agcgttcgtt ttctcacccg cgtaggacgt
     2221 cagcggctcc gccgatcaat cgccgatcta ctcccacatc gcgcgcaacg ccgctgcagc
     2281 atcgactcgt acgttcgcct tcgtgtccaa agcacgcagc gccgactcgc gagcagcggg
     2341 ccaggccttc tgctcgaact gcgccatcag ctgatcggtc atgaaacgac tgcgcgcgcc
     2401 gtaaacatgt gcgtcgcccg tgcggctctg ctgcgtgatg tagtacttga gcggcgcgat
     2461 caaatgcagg tcgcgcttcg cgcgcgtcat cgcgacgtac aacagcctgc gctcttcttc
     2521 gatgagatcg ggccggcctg cggcatgctc gttcgggaaa ttgccgtcgg agacgttgag
     2581 cacgtacacc gaatcccact cctgtccttt cgccgaatgc acggtggaaa gaatcaaata
     2641 gtcctcgtcg agcagcgggt cgcccgcgag atcgccgctg acgttcggcg gatcgagcgt
     2701 cagctcggtg acgaactgct cgcgcgtcgc gtattgaccg gacaggcgct ccagttg
//
LOCUS       DATANS010000244         1552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_598716, whole genome shotgun sequence.
ACCESSION   DATANS010000244 DATANS010000000
VERSION     DATANS010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598716"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VHT22_03440"
     CDS             <1..864
                     /locus_tag="VHT22_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4743 domain-containing protein"
                     /protein_id="HEX3136636.1"
                     /translation="IDGHTVGWIDPARTERLSAFASVFVRAEGEIRLAATLAGRNART
                     AAVDAVTRALAGEGLLSAWRDERYAIAVEFGAAPLVDIERAAARYFGIRTYAVHVNGL
                     VRNGDDLAMWLARRSPTKAIDPGLLDNLVGGGIATGYGVLDTLRKEAWEEAGIPASVA
                     ARARPEGSIDICRAQPQGLQRETVFVYDLELDADFRPANQDGEAVEHRQLTMSDAAKL
                     IAADGGDDAVTADASLVILDCLLRHGALGPDRSDIRALSAFRHAVLEPAAQKNGRGAW
                     PPGAQSRPTRP"
     gene            complement(851..>1552)
                     /locus_tag="VHT22_03445"
     CDS             complement(851..>1552)
                     /locus_tag="VHT22_03445"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEX3136637.1"
                     /translation="GILNPLPEIAEAVAQRGRRLIIDAMSSFGAIPIDAKAVPFDALI
                     AASGKCIEGPPGMGFVFARKSVLEQCAGNSSSLSLDLYDQWAYMEKTTQWRYTPPTHV
                     VVALDAALEQYLAAGGQPARLARYTANCETLIAGMKEMGFRPFLDARIQAPIIVTFHA
                     PADPRYAFKAFYDKVRDKGFILYPGKLTQLETFRVGCIGAIGPYEMRHAVNAVRDALA
                     DLGIRQIAPATVKAA"
BASE COUNT          238 a          531 c          533 g          250 t
ORIGIN      
        1 atcgacggcc acaccgtcgg ctggatcgac cccgcgcgca ccgagcgcct gtccgccttc
       61 gcttcggtat ttgtacgtgc tgaaggcgag atccggcttg ccgcgacgct cgccggccgc
      121 aacgcgcgaa ccgctgccgt cgacgcggtg acgcgcgcgc ttgccggcga gggtctgctc
      181 tccgcgtggc gcgacgagcg ctatgcgatc gccgtcgagt tcggcgccgc gccgctcgtc
      241 gacatcgagc gcgcggcggc gcgctatttc ggcattcgca cctacgccgt gcacgtcaac
      301 gggttggtgc gcaacggcga cgacctggcg atgtggctcg cgcggcgcag tccgaccaag
      361 gcgatcgatc ctggccttct ggacaatctg gtcggcggcg gcatcgccac cggctacggc
      421 gtcctcgaca ccctgcgcaa ggaggcgtgg gaggaggccg gcatcccggc cagcgtcgcc
      481 gcgcgggcgc gcccggaggg cagcatcgac atctgccggg cgcagccgca gggcctgcag
      541 cgcgaaaccg tattcgtcta tgacctcgag ctcgatgctg atttcaggcc ggcgaatcag
      601 gatggcgagg cggtcgagca ccggcagctg acgatgtccg acgcggcgaa actgatcgcc
      661 gccgacgggg gcgatgacgc agtcacggcc gacgccagcc tggtcatcct cgactgcctg
      721 cttcgccacg gcgcgctggg accggatcgc tccgacattc gcgcactgtc ggcattccgg
      781 cacgcggtgc tcgagcccgc ggctcagaaa aacggccgcg gtgcctggcc gcctggcgcg
      841 cagtcccggc ctacgcggcc ttgacggtgg ccggcgcgat ctggcggatg cccagatccg
      901 ccagggcgtc gcgcaccgca ttgacggcgt gacgcatctc gtagggccct atcgcgccga
      961 tgcacccgac ccggaaggtt tcgagctgcg tcagcttgcc gggatagaga atgaagccct
     1021 tgtcgcgcac cttgtcgtag aacgccttga acgcatagcg cgggtccgcc ggcgcgtgga
     1081 aggtcacgat aatcggcgcc tggatgcgcg catcgagaaa cggccggaag cccatttcct
     1141 tcatccctgc gatcagggtc tcgcagttgg ccgtatagcg cgcgagccgc gccggctggc
     1201 ctcctgcggc cagatattgc tcgagcgcgg catcgagggc gacgacgaca tgcgtcggcg
     1261 gcgtgtagcg ccactgtgtc gtcttttcca tgtacgccca ttggtcgtac agatcgagcg
     1321 acagcgagga cgagttgccg gcgcattgct cgagcacgct tttccgcgcg aagacgaagc
     1381 ccatgcccgg cggaccttcg atgcactttc ccgacgcggc gatcagcgcg tcgaagggaa
     1441 ccgccttcgc gtcgatagga atcgcgccga acgagctcat cgcgtcgatg atcaggcgcc
     1501 ggccgcgttg cgctaccgct tcggcgatct ccggcagcgg gttgaggatt cc
//
LOCUS       DATANS010000245         1695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_96823, whole genome shotgun sequence.
ACCESSION   DATANS010000245 DATANS010000000
VERSION     DATANS010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96823"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1599)
                     /locus_tag="VHT22_03450"
     CDS             complement(157..1599)
                     /locus_tag="VHT22_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX3136638.1"
                     /translation="MIAKDLPYLPLTELSRRLSAGETSSHDIVTACIERIAALDGHAH
                     AFIEVYRQAALDGAAAADLQRKAGLGRGPLHGLPIALKDLLHVKDRVTTAGSKSWRGR
                     VATETATAVERLLAAGMIPLGKTHMVEFAFGGWGRNEPMGAPWNPWDTRVHRVAGGSS
                     SGSAVAVAAGLAPAAIGSDTGGSIRIPAALCGLTGLKPTYGTISLAGVVPLATTLDSL
                     GPLARTVEDAALLTAAMAGPDPRDAATQNLPRLDFDAVLAGAPDVRCLRITALPAEQL
                     PPDTLPDVARALQQAIAVLRELGALVDEVTVPIDFEDLMVRNGRIIAAEAWALHRDYI
                     EDPKLPIDPWVRKRTIGGKAISAADYIEELARRKRAAAAFAEWMRGRDVLLTPTLPIT
                     ATAVDEVDEVATPLATFTRVANYLGICALSLPAGISADGLPVAVQLLGAPFAEATLIR
                     AGRAFQQATDWHLQRADVSAWETALPRDRH"
BASE COUNT          258 a          576 c          590 g          271 t
ORIGIN      
        1 ggtcgcaagc gccatgccag gcggggccga tcgacgaata tgccaaataa cagagactta
       61 ccgcgatcat cgtcgctgga aaggtctcgc ccctgcactg ttgccatgca acgggggtgt
      121 tcggggcacc acgcttgtgc gccgagcttc tcgaaactag tggcggtcgc gcggcagcgc
      181 ggtttcccac gcggagacat ccgcacgctg cagatgccag tcggtggcct gctggaatgc
      241 gcggccggcg cggatcagcg tcgcttcggc gaatggcgcg ccaagcagtt gcaccgcgac
      301 cgggagccca tcggcagaga tgccggccgg cagcgacaac gcgcagattc cgaggtaatt
      361 ggcgacccgt gtgaacgtgg cgagcggtgt ggccacctcg tcgacttcgt cgaccgccgt
      421 ggcggtgatc ggtagagtcg gcgtcagcag gacgtcgcgg ccgcgcatcc attcagcgaa
      481 cgcggcggcg gcgcgcttgc gccgggcaag ctcctcgata tagtccgcag cgctgatcgc
      541 cttgccgccg atcgtgcgct tgcgcaccca cggatcgatc ggcagcttgg ggtcttcgat
      601 gtagtcgcgg tgcagcgccc acgcctcagc ggcgatgatc ctgccgttac gcaccatcag
      661 gtcctcgaag tcgatcggca cggtgacttc gtcgaccagc gcgccgagct cgcgcagcac
      721 agcgattgcc tgctgcaagg cgcgcgccac atccggcagc gtatcgggtg gcagctgctc
      781 ggccggcagc gccgtgatgc gcaggcagcg cacatcgggc gcgccggcga gcactgcgtc
      841 gaaatcgagc cgcggcaggt tctgcgtggc cgcgtcgcgc ggatccgggc cggccattgc
      901 cgcggtcagc agcgcggcgt cctcgacggt gcgcgccagc ggcccgagcg agtcgagcgt
      961 cgtcgccagc ggcacgacgc cggcgaggct gatcgtgccg taagtcggct tcagcccggt
     1021 caacccgcag agtgctgcgg ggatgcgtat cgatccgccg gtgtccgagc cgatcgcggc
     1081 gggtgccagg cccgcggcga ccgcgaccgc ggacccgctg ctcgagccac cggcgacgcg
     1141 gtgcacgcgc gtgtcccacg gattccacgg cgcgcccatt ggctcgttgc gcccccaccc
     1201 gccgaaagcg aattcgacca tgtgcgtctt gccgagcgga atcatccctg cggcgaggag
     1261 cctctcgacc gcggtggcag tttcggtcgc cacccgtccg cgccagctct tcgagcccgc
     1321 ggtcgtcacc cggtccttga cgtgcagcag atccttcagc gcgatcggca gtccgtgcag
     1381 cggaccgcgg cccagtccgg ccttgcgctg caggtcggct gcggccgcgc cgtcgagcgc
     1441 ggcttggcga tagacctcga tgaatgcatg cgcgtgtcca tcgagcgccg caatgcgctc
     1501 gatgcaggcg gtgacgatgt cgtgcgacga cgtttccccg gcgctcaggc ggcgcgacag
     1561 ttcggtcagc ggaagatacg gaaggtcttt cgcgatcatg cggtcgcgcc tttatgcggg
     1621 aaattcgctg gacattcgga tcatagccga gcgcggcgcg ccaagtctgc cgtcatcatc
     1681 gatcgtgttc aagcc
//
LOCUS       DATANS010000246         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_222268, whole genome shotgun sequence.
ACCESSION   DATANS010000246 DATANS010000000
VERSION     DATANS010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222268"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2018
                     /locus_tag="VHT22_03455"
     CDS             <1..>2018
                     /locus_tag="VHT22_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012166113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3136639.1"
                     /translation="LEFLVGLSLQATVAIENARLFAESQQRAAELTTVNTVSQQLAGK
                     LDLANLLELVGEQIRTVFRADIAYVALLDRRTGIIDFPYQHGDKLTPLKYGEGLTSRI
                     IKSGKPLIINRDTDRRTLELGAVVVGKQALSYLGVPILVAGTCQGAISVQSTLMEGVY
                     DADSERLLSTIAANVGVALQNARLFQETQEARAAAEAANEAKSSFLATMSHEIRTPMN
                     AVIGMSGLLLDTKLDAEQHDYVATIRESGDALLTIINDILDFSKIEAGRMDIEAQPFD
                     LRECVESALDLVTARAVEKNLDTVYVFEGDIPAAVVGDVTRLRQIMLNLLSNAVKFTE
                     AGEVVMTVIAKPMSSDQVELTFSVRDTGIGLSADEMGRLFQSFSQADSSTTRKYGGTG
                     LGLAISRRLSELMGGRMWAESDGLGKGSTFSFTIQVPVTELPAARQRDFVGVQPELQG
                     KRLLVVDDNATNRRVLSLQAAKWGMKCRATGSPSEALSWLAGGDEFELAVLDMHMPEM
                     DGIELARRIREQNTVLPLVLFSSLGRREAGDDERLFNAYLAKPIRQSQLFDTLVGLLA
                     RDLAPKVEAAPGKPQLDPNQAARHPLRILLAEDNVVNQKLALRLLQQMGYRADLASNG
                     IEAIESVRRQTYDVVLMDVQMPEMDGLEASRQINARWQPGERPRIIAM"
BASE COUNT          373 a          633 c          668 g          344 t
ORIGIN      
        1 atctcgaatt cctggtcggg ctgtcgctgc aggcgacggt cgccatagag aatgcgcgcc
       61 tgttcgccga atcgcagcag cgcgcggcag agctgaccac ggtcaacacg gtctcgcagc
      121 agttggccgg gaagctcgat cttgcgaatt tgctcgagct cgtcggcgag cagattcgca
      181 cggtcttccg ggccgatatc gcgtatgtcg cactgctcga ccggcgcacc ggcatcatcg
      241 attttcccta ccagcatggc gacaagctga cgccgctcaa atatggcgaa ggcttgacca
      301 gcaggatcat caagtcgggc aagccgctga tcatcaaccg cgataccgac cggcgcactc
      361 tcgagctggg cgccgtcgtc gtcggcaagc aggcgctgtc gtatctgggg gtgccgatcc
      421 ttgtggcggg cacttgtcag ggagcgatca gcgtgcaaag cacgttgatg gagggcgtct
      481 acgacgccga ttccgagcgc ctgctctcca cgatcgccgc caatgtcggc gtcgcgctgc
      541 agaatgcgcg actgttccag gaaacgcagg aggcgcgtgc cgccgccgag gccgcgaacg
      601 aagcgaaaag ctcgttcctg gcgacgatga gccacgagat ccgcacgccg atgaacgcgg
      661 tgatcggcat gagcggcctg ctgctcgata ccaagctcga tgccgagcag cacgactacg
      721 tcgcgaccat tcgcgaatcg ggcgacgcgc tgctgaccat catcaacgac atcctggact
      781 tttcgaagat cgaagcgggc cgcatggaca tcgaggcgca gccgttcgat ctgcgcgagt
      841 gcgtcgagtc ggctctggat ctggtcacag cgcgcgcggt ggaaaagaac ctggacacgg
      901 tctatgtatt cgagggcgat attccggccg ccgttgtcgg cgacgtgacg cgcctgcgcc
      961 agatcatgct gaacctgctg tcgaatgcgg tgaaattcac cgaggccggc gaggtcgtga
     1021 tgaccgtcat tgccaagcct atgtcgtcag atcaggtcga actcaccttt tcggtgcgtg
     1081 acaccgggat cggtttgtcg gccgacgaga tgggtcgcct gttccagtcg ttctcgcagg
     1141 ccgactcgtc gaccacgcgc aaatacggcg gcaccggcct cggcctcgcg atcagcaggc
     1201 ggctctccga attgatgggc ggccgcatgt gggcggaaag tgatgggctc ggcaaagggt
     1261 ccaccttctc gttcaccatc caggtgcccg tcaccgagct ccccgcggcg cggcagcgcg
     1321 acttcgtcgg ggtgcagccc gagctgcaag gcaagcggct gctggtcgtc gacgataacg
     1381 ccaccaatcg ccgcgtactt agcctgcagg ccgcgaagtg gggaatgaag tgccgcgcga
     1441 ccgggtcgcc ctccgaggcg ctgtcgtggc tcgcgggcgg cgatgaattc gagctcgcgg
     1501 tgctggacat gcacatgccc gaaatggacg gcatcgagct ggcacggcgt attcgcgagc
     1561 aaaacaccgt gctgccgctg gttctgttca gctcgctggg aagacgcgaa gcgggcgacg
     1621 acgagcggct gttcaacgcc tacctggcca agccgattcg ccagtcgcag ctgttcgaca
     1681 cgctggtggg cctgcttgcg cgcgacctcg cgcccaaggt cgaggcagcg ccgggcaagc
     1741 cgcagctcga ccctaaccag gcggcgcggc acccgctgcg catcctgctc gccgaagaca
     1801 acgtggtcaa ccagaagctg gcgctgcgcc tgctgcagca gatgggttac cgcgccgacc
     1861 tcgcgtcgaa cggcatcgag gcgatcgaat cggtgcgccg acagacctac gatgtcgtgc
     1921 tgatggacgt gcagatgccc gagatggatg gattggaagc ctcgcgtcag atcaacgcgc
     1981 gatggcaacc gggcgagcgg ccgcgcatca ttgcgatg
//
LOCUS       DATANS010000247         1879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_516952, whole genome shotgun sequence.
ACCESSION   DATANS010000247 DATANS010000000
VERSION     DATANS010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516952"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /gene="lptG"
                     /locus_tag="VHT22_03460"
     CDS             complement(<1..1025)
                     /gene="lptG"
                     /locus_tag="VHT22_03460"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEX3136640.1"
                     /translation="MKTLNRYVGREVVYATALIFVALLMLFAFFDLIHELGNVGRDGY
                     PPGRALLFVGLSLPGHVYELMPTAALIGTLFAIAQLVGNSEYTVMRASGASLTQITWA
                     IVRVGIPLAIATFLAGEFLAPPAERLAQQVRVQTQGQALRFVAQQFQSGFWFKQTLPD
                     SDVQTFANIRSLLADMTLLGVRIYEFDADLRLKALRVADSGSFVADGEWKLKNVRSTE
                     LGPDGAKVTQSSEYVWATVLKPSLLTVYQVAPEKLEIESLYDNIRVLGGNQQKTSRFE
                     IALWNKIFYPAAVVVMMILALPFAYFQRRAGGIGFRIFAGTILGLVFFLVGRLFSYLG
                     VINDWPPL"
     gene            complement(1022..>1879)
                     /gene="lptF"
                     /locus_tag="VHT22_03465"
     CDS             complement(1022..>1879)
                     /gene="lptF"
                     /locus_tag="VHT22_03465"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEX3136641.1"
                     /translation="SRWYRDSEMIVWLTSGQSLTAWLKPILWFATPFLLAIVALSLWL
                     SPWAEARRLEYERQLESREELSLLTPGLFKEFRQAKLVVFIESINTLDGTIKNVFLHS
                     IDSDKDVTTVARSGRLEEAPNGDRFIVLQDGRRYEGTPGSADFRTAEFERLGRRIEPA
                     EVRALPASLKALPTDVLALADGAPERAELFWRISVPISALLLVLLAVPLAYVNPRMGR
                     SLNLFIALFMYMLYSNCLNIMQSTIAQGRLSLVAGLLIVHSVAAAVVVLLFLKRLSVR
                     GWFPRRRLA"
BASE COUNT          385 a          590 c          614 g          290 t
ORIGIN      
        1 agcggcggcc aatcgttgat gacgccgaga taggaaaaca ggcgtccgac caggaaaaag
       61 accaggccca ggatcgtccc ggcaaagatg cgaaagccga tgccgccggc gcgacgctgg
      121 aaataggcga aaggcagcgc caggatcatc atgacgacca cggccgccgg gtagaagatc
      181 ttgttccaca gcgcgatctc gaagcgcgag gttttctgct gattcccgcc caatacgcga
      241 atgttgtcgt acaaggattc gatctccagc ttttccggcg cgacctggta aacggtcaaa
      301 agcgaaggtt tcaggacggt cgcccagacg tactcgctcg actgcgtgac cttggcgcca
      361 tccggtccca attcggtcga gcgcacgttc ttgagcttcc attcaccgtc ggcgacgaag
      421 ctgccggaat cggcgacgcg caatgccttc aggcgcaggt cggcgtcgaa ctcgtagatc
      481 cgcacgccta gcaaggtcat atcggcgagc aggctgcgga tgttggcaaa agtctggacg
      541 tcgctgtccg gcagcgtttg cttgaaccag aaacccgact ggaattgctg cgcgacaaag
      601 cgcagcgcct ggccctgcgt ctgaacgcgg acctgctgcg ccaggcgctc ggccggaggt
      661 gccaggaatt cgccggcgag aaacgtggcg atggcgagcg ggatgccgac ccgcacgatc
      721 gcccaggtga tttgcgtcag cgacgcgccc gaagcgcgca ttaccgtgta ctcggaattg
      781 ccgaccagct gcgcgatcgc gaacagcgtt ccgatcagcg cggccgtcgg catcagctcg
      841 tagacgtggc cggggaggct caagccgacg aatagcaacg cgcggccggg aggatagccg
      901 tcgcgtccga cgttgccgag ctcgtggatc agatcgaaga acgcgaagag catcagcagc
      961 gcgacgaaaa tcaaggccgt cgcatagacc acctcacgcc cgacgtagcg gttgagcgtc
     1021 ttcacgcgag gcggcgccgg gggaaccagc cgcgcacgga cagccgcttg aggaacagca
     1081 ggacgacgac cgccgccgcg accgaatgga cgatgagcag ccccgccacc agcgacaagc
     1141 ggccttgcgc aatggtgctc tgcatgatgt tcaggcagtt gctgtacaac atgtacatga
     1201 acaacgcgat gaacaaattg agcgagcggc ccatccgcgg attgacgtag gcgagcggga
     1261 ccgccagcag cacgagcagc agggcactga tcggcaccga tatgcgccag aacagctcgg
     1321 cccgctcggg cgcgccgtcg gccagcgcca gcacgtcggt cggcagcgcc ttcagcgacg
     1381 cgggcagcgc gcgcacttcg gcgggctcga tccggcgtcc gagacgctcg aactccgccg
     1441 tgcggaaatc ggcgcttccg ggggttccct cgtaacggcg gccgtcctgc agcacgatga
     1501 agcggtcgcc gttgggcgcc tcttccaacc gacccgagcg ggcgacggtg gtcacgtcct
     1561 tgtcgctgtc gatcgaatgc aggaacacgt tcttgatcgt tccgtcgagc gtgttgatgc
     1621 tttcgatgaa cacgaccagc ttcgcctggc ggaattcctt gaacagtccc ggcgtcagca
     1681 gcgacagctc ttcgcgcgac tcgagctggc gctcgtattc gagccgccgc gcctcggccc
     1741 agggtgaaag ccaaagtgag agcgcgacga tcgccagcag gaatggcgtg gcgaaccaga
     1801 gtatcggctt gagccaggcg gtcaggctct ggcccgaggt cagccagacg atcatctcgc
     1861 tgtcgcgata ccagcgcga
//
LOCUS       DATANS010000248         1948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_181871, whole genome shotgun sequence.
ACCESSION   DATANS010000248 DATANS010000000
VERSION     DATANS010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181871"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VHT22_03470"
     CDS             complement(<1..363)
                     /locus_tag="VHT22_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEX3136642.1"
                     /translation="MAGAHAAPPQRIVSLAPHLTELAFTAGAGDRIVATVEYSNHPDA
                     ARSIPRIGDAFRVDLERLVALRPDVVLVWQTGMPEPTIERIRALQLRVVPFQTQRLAD
                     VATVLREIGRIAGTSEIAE"
     gene            complement(417..1025)
                     /gene="ampD"
                     /locus_tag="VHT22_03475"
     CDS             complement(417..1025)
                     /gene="ampD"
                     /locus_tag="VHT22_03475"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEX3136643.1"
                     /translation="MEKASWRIVTTMTISVDSSTGLMQPARQVASPNHDERPGGMLPE
                     LILVHSISLPPDEYGGPWIDRLFTNTLPPDAPEVHPFFATVMTLEVSAHLLIRRDGAY
                     VQYVPFHLRAWHAGASNYCGRERCNDFSIGIELEGSDELPYEPIQYRALSSAIHALCR
                     AYSSLSLERVVGHSDVAPGRKTDPGPAFDWPRLRALLRLPRG"
     gene            1024..1758
                     /locus_tag="VHT22_03480"
     CDS             1024..1758
                     /locus_tag="VHT22_03480"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019332939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX3136644.1"
                     /translation="MRTNRIFCEGPLQSGAEIALAPAGAYHVARVLRMREGAPLSVFD
                     GGGQEFRAEIVRVAGDDVTVRLNEATAVAPESPLRITLLQGVSRSERMDWTLQKATEL
                     GVAAIAPVLTARSVVRLDEKQAQKKQSHWRGIVIAACEQCGRSKIPLVAAPVTLREHL
                     ANARKEGLRLVLSPSAPGSLAGLASLPNKVELLIGPEGGLDDDELQAAQKAGFMAVRL
                     GPRVLRTETAAVVALTVLQALWGDLR"
     gene            complement(1762..>1948)
                     /locus_tag="VHT22_03485"
     CDS             complement(1762..>1948)
                     /locus_tag="VHT22_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136645.1"
                     /translation="VITQGFPQLVRVNADVVKHDPSRNFPERGPILCQVRMLNESPLI
                     PDFECLEQMISEETRAG"
BASE COUNT          349 a          607 c          690 g          302 t
ORIGIN      
        1 ctcggcaatc tcgctcgttc ccgcgatgcg gccgatctcg cgcagtaccg tcgcgacgtc
       61 ggcgaggcgc tgcgtctgga atggcacgac gcgcagctgc agcgcacgaa tgcgttcgat
      121 ggtgggctcg ggcatgcctg tttgccagac gaggaccaca tcaggccgca gtgcgaccag
      181 gcgctccaga tcgacgcgga aagcatcgcc gatgcgggga atcgagcgcg cagcgtcggg
      241 atgattgctg tattcgacgg tcgcgacgat gcgatcgcca gcgccggcgg tgaacgcaag
      301 ctcggtgaga tgcggagcga gactgacgat gcgctgaggc ggcgcggcgt gagcgcctgc
      361 caccagagcg aagaaaagag tcaggctgca gcctgaccca cagagacggc gcggtttcat
      421 ccgcgtggca aacgcagcag cgcgcgcagc cgcggccaat cgaatgcggg gccgggatcg
      481 gtcttgcggc ccggcgcgac gtcgctatgc ccaacgacgc gctcgagcga aagcgatgaa
      541 tacgcgcgac acagcgcgtg aatggcgctg ctcagcgcac gatactggat cggttcgtac
      601 ggcagctcat cgctgccttc cagttcgatg ccgatcgaga aatcgttgca gcgctcgcgc
      661 ccgcaatagt tcgatgcgcc ggcgtgccac gcgcgcaaat ggaacggcac gtactgcacg
      721 tacgcgccgt cgcgacggat cagcagatgc gcagaaactt ccagggtcat caccgtcgcg
      781 aagaacggat gcacctcagg tgcgtccggc ggcagcgtgt tggtgaacag gcgatcgatc
      841 cacggcccgc cgtactcgtc cggcggcaga ctgatgctgt ggacgaggat cagctcgggc
      901 agcatgccgc ccggccgctc gtcgtggttc ggcgaggcga cctggcgcgc gggttgcatc
      961 agcccggtgc tcgagtcgac cgaaatagtc atcgtagtca cgatgcgcca tgatgccttc
     1021 tccatgagaa cgaatcgaat cttctgtgaa ggaccgttgc agagcggcgc cgagatcgcg
     1081 ctcgcgccag cgggcgccta tcacgtcgcg cgtgtcctgc ggatgcgcga aggcgcgccg
     1141 ctctcagtat tcgacggcgg tggccaggag ttccgtgcag aaattgtccg cgtcgcgggc
     1201 gacgacgtga ccgtgcgatt gaacgaagcc accgcggtcg cgccggaatc gccgctgcgg
     1261 atcacgctgc tacaaggcgt atcgcgcagc gagcgcatgg attggacgct gcagaaagcg
     1321 accgagctcg gcgtcgccgc gatcgcgccg gtgctgaccg cgcgcagcgt cgtgcgactg
     1381 gacgagaagc aggcgcagaa gaagcagagt cattggcgcg gcatcgtgat cgcggcatgc
     1441 gagcaatgcg gccgatcgaa aatcccgctg gttgcagcac cggtcacgct gcgcgagcac
     1501 ctggcgaacg cgcgcaagga aggactgcgg ctcgtcttga gcccatcagc gcccggctcg
     1561 ctcgcgggcc tggcgagcct gccgaacaaa gtggagctgc tgatcggccc cgagggcggc
     1621 ctggatgacg atgagctgca ggccgcgcag aaggcgggct tcatggcggt gcgcctcggg
     1681 ccgcgcgtgc tccgaactga gacagcggcg gtcgtggcgc tgacggtgct acaggcgttg
     1741 tggggagatc tgcggtagct gtcagccggc tctggtctct tccgagatca tctgctccag
     1801 gcactcgaag tccgggatca gcggcgactc gttgagcatg cggacctggc acaggatcgg
     1861 gccgcgctcc gggaagttgc ggctcgggtc gtgcttcacc acgtcggcgt tcacgcgcac
     1921 cagctgcggg aagccctgcg tgatcacg
//
LOCUS       DATANS010000249         1965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_307389, whole genome shotgun sequence.
ACCESSION   DATANS010000249 DATANS010000000
VERSION     DATANS010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307389"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            396..602
                     /gene="thiS"
                     /locus_tag="VHT22_03490"
     CDS             396..602
                     /gene="thiS"
                     /locus_tag="VHT22_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX3136646.1"
                     /translation="MVSISLNGAMYVCRSASPRVADLVRELALEGKRVAVEKNGQIVP
                     RSRHAETMISAGDRIEVVAAVGGG"
     gene            614..1429
                     /locus_tag="VHT22_03495"
     CDS             614..1429
                     /locus_tag="VHT22_03495"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX3136647.1"
                     /translation="MNAPTENVALAHDDPLVIAGAAYRSRLLVGTGKYKDFAATRAAI
                     DASGAEIVTVAIRRTNIGQNPNEPSLLDHLPPSDFTLLPNTAGCYTADDAVRTLRLAR
                     ELLDGHKLVKLEVLGDAHSLFPDVRQTIRAAETLVADGFDVMVYTSDDPIIARELEAV
                     GCVAIMPLASLIGSGMGILNPWNLKLIVEQAKVPVLVDAGVGTASDAAIAMELGCAGV
                     LMNTAIALAKDPVLMAGAMKKAVEAGREAFRAGRMPRKFYAASPSSPTTGLIA"
     gene            1426..>1965
                     /locus_tag="VHT22_03500"
     CDS             1426..>1965
                     /locus_tag="VHT22_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136648.1"
                     /translation="MSRIATTLGLSPARRAWGALFCCLPLFAQAAPLTRAEIEATCTG
                     AEDAAHCGRLIEALQLPRLPGLARRNGNGLIVSLFPSGSTTFTDSDDPVNGRSYSLWD
                     YFDGINSVVLYVTVGDDASFALLQRATGRLVDLPAEPRLSPDRQRFAVADVCAQHCGN
                     EIAVWRVTRDGFRKELRWTP"
BASE COUNT          305 a          667 c          648 g          345 t
ORIGIN      
        1 cctgtgggtc attctctatc gggccaggat ggcgctccgg caatgcctcg agcagcactg
       61 gtttgccgat ccctcccgcg ccggctgatg tcgtcgattg cctaagtcat gggccccctt
      121 atttcgtgca aggacgcgag ccggctgatc tcgcagatgc aggaccagag cctgacgcct
      181 ggaaagcgtt ggctggtccg tctgcatctg ttgttttgcg acgcctgccg gcatttcgag
      241 cgtcaactcg cggttctcag agaagcaatg ctccgctatc gcggctaagc gtagccgggc
      301 ggcagccgtc cggcgccgac gcggggcgcc agcggccggc ttgaaccggg ccccggtagt
      361 gataatctct actccgtaac gatggctcga cgctgatggt ctcgatttcc ctcaatggcg
      421 caatgtacgt ctgccggtcc gcctcgcccc gggtggccga tctcgtccgc gagctggcgc
      481 tggagggcaa gcgtgtcgcg gtcgagaaga acggccagat cgttcctcgc agccgacacg
      541 cggaaaccat gatcagcgcc ggagatcgaa tcgaggtcgt cgccgccgtc ggcggcggct
      601 aggtcttagg aagatgaacg ctcccaccga aaatgtcgcc ctggcgcacg acgacccgct
      661 ggtgatcgcc ggggccgcct atcgctcgcg cctgctggtc ggcaccggca agtacaagga
      721 cttcgccgcg acccgggcgg cgatcgacgc ctccggtgct gagatcgtca ccgtggcgat
      781 tcgccgcacg aacatcggcc agaatccgaa cgagccgtcg ctgctcgacc acctgcctcc
      841 gtcagacttc acactgttgc cgaacaccgc cggctgctac acggcagacg acgcggtgcg
      901 aacgctgcgg ctcgcacgcg aattgctcga cgggcacaag ctggtcaagc tcgaggtcct
      961 gggcgatgcg cattcgctgt tccccgatgt gcggcagacg atacgcgcgg ccgagacgct
     1021 ggtcgccgat ggcttcgacg tcatggtcta cacctccgac gatccgatca tcgcgcgcga
     1081 gctcgaagcc gtgggttgcg tcgccatcat gccgctggcg tcgctgatcg gctcgggaat
     1141 ggggatcctc aacccctgga acctgaagct gatcgtcgag caggcgaaag tgccggtgct
     1201 ggtcgatgcc ggggtgggca ccgcttccga cgccgctatc gcgatggagt tggggtgcgc
     1261 cggcgtgctg atgaacaccg ccatcgccct ggcgaaggac ccggtgctca tggcgggcgc
     1321 gatgaaaaaa gccgtcgagg cgggccgcga agcgtttcgt gccggccgca tgccccgcaa
     1381 gttctacgcg gccagcccca gctcgccgac gaccggcctc atcgcttgag ccgcatcgcc
     1441 acgacgctcg ggctctcgcc ggcccggcgc gcgtggggcg cgctattctg ctgtcttccg
     1501 ctgttcgccc aggccgcgcc gctgacccgc gccgagatcg aggcgacctg caccggcgcg
     1561 gaagacgccg cgcattgcgg ccggttgata gaagccctgc agctcccccg cctgccgggc
     1621 ctcgcccgcc gcaacggcaa cggattgatc gtctcgctct ttccgtcggg cagcacgacc
     1681 ttcaccgaca gcgacgaccc ggtcaacggc cgctcgtaca gtctctggga ttatttcgat
     1741 ggcatcaaca gcgtcgtgct ctacgtgacc gtcggtgacg atgcgagctt tgcgctgctg
     1801 cagcgcgcga ccggacggct ggtcgatctg ccggcggagc cgcggctttc gcccgaccgg
     1861 cagcgtttcg ccgttgccga tgtttgcgcg cagcattgcg gcaacgagat cgcggtctgg
     1921 cgggtcacgc gcgacggttt tcgcaaggag ctgcgctgga cgccc
//
LOCUS       DATANS010000250         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_139167, whole genome shotgun sequence.
ACCESSION   DATANS010000250 DATANS010000000
VERSION     DATANS010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139167"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VHT22_03505"
     CDS             <1..450
                     /locus_tag="VHT22_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein"
                     /protein_id="HEX3136649.1"
                     /translation="DEGLRSVPRDTEILVIDAPARTHGSEMNELVRRAETILVPVLPS
                     PIDMKACAYFMTELLELGKVQRQQARLAVVANRVRENTLMYEELDQYLGKLKVPYLAS
                     LRQSANYLRAFQRGMGIFELPEYLAWPDWKQWEPVTKWLGSKKSQPS"
     gene            complement(459..1121)
                     /locus_tag="VHT22_03510"
     CDS             complement(459..1121)
                     /locus_tag="VHT22_03510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3136650.1"
                     /translation="MAFKTREAEILRGNARLPTDASTKSPLQRLFTDPDFTARVVRHL
                     LHLPVRMETEDRRVLENLIFPYYRALPGLRSILFVGCDWYTRHYERAFFRNTDYWTID
                     LSPRARRFAGRRHVVGPLERLDEFFPEQRFDVILCNGVYGFGLDGHQQCERAFELCRS
                     RLVDGGHFLLGWDDIPARTPVPLREIASLKRFRTFLFPPLGVCRYVTDTPYRHTYDFY
                     CK"
     gene            complement(1195..2085)
                     /locus_tag="VHT22_03515"
     CDS             complement(1195..2085)
                     /locus_tag="VHT22_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX3136651.1"
                     /translation="MQANRRQVLIAGCGYVGQRLALRLQERYDVTGLVRSAEHAAELE
                     RLGVRALAIDLDRVRAGTTIPESLDLEAIVYLTPPPVLGESDLRLDRFLHLASVPPHT
                     FVYMSTTGVYGDTAGAVVDESSVVQPRTDRARRRISAEEMTRVWCTERRVRRVVLRVP
                     GIYGPDRLPLERLRHYEPVVREREAGINNRIQVEDLVEACTCAITNSEARGVYNITDG
                     RPLSSTTFINMVAKVAGLPAPPQVSMEEAQLTFSPERLSFLNESRRVSNERMLKHLGV
                     TLKYADVEAGIRASMPVAGA"
     gene            2145..>2451
                     /locus_tag="VHT22_03520"
     CDS             2145..>2451
                     /locus_tag="VHT22_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3136652.1"
                     /translation="MKLTPTVQRCILHWGEMAGRWGVSRSVAQIHALLFLAPQPLTAD
                     DIAETLAVARSNVSVSLKELQQWDLVSVTHVLGDRRDYFQARKDIWEVLAMIMDGRKK
                     "
BASE COUNT          456 a          775 c          816 g          404 t
ORIGIN      
        1 gacgaaggcc tgcgatccgt gccgcgcgac accgagatcc tggtcatcga cgcgccggca
       61 agaacgcacg gctccgagat gaacgagctg gtgcgccgcg cagagacgat cctggtgccg
      121 gtgctgccgt cgccgatcga catgaaagcc tgcgcgtact tcatgaccga gctgctcgag
      181 ctgggcaaag tccagcggca gcaggcgcgg ctcgcggtcg tggcgaaccg cgtgcgcgag
      241 aacacgctga tgtacgaaga gctggatcag tacctgggca agctcaaggt gccgtacctg
      301 gcctcgctgc ggcagtccgc caactatctg cgcgcgttcc agcgcggcat ggggatcttc
      361 gagctgccgg aatacctggc gtggccggac tggaagcagt gggaaccggt cacgaagtgg
      421 ctggggagca agaagagtca gccgagctga cccggccgtc acttgcaata gaaatcgtag
      481 gtatggcgat acggcgtgtc agtgacgtag cgacagacgc cgagcggcgg aaacaggaac
      541 gtccggaagc gcttcaggct cgcgatctcc cttagcggca ccggcgtgcg tgccggaatg
      601 tcatcccagc cgagcaggaa gtgtccgccg tccaccagcc gcgagcggca gagctcgaac
      661 gcgcgctcgc attgctggtg tccgtcgagg ccgaatccgt acaccccgtt gcagaggatg
      721 acgtcgaagc gctgctccgg aaagaactca tcgagcctct cgagcggtcc gacgacgtgg
      781 cgccggccgg cgaagcgccg cgcgcgaggc gacagatcga tggtccagta gtccgtgttg
      841 cggaagaacg cgcgctcgta gtggcgcgtg taccagtcgc agccgacgaa gagaatcgag
      901 cgcagaccgg gcagagcccg gtagtaggga aaaatcagat tctccagaac gcggcgatcc
      961 tcggtctcca tgcgcacggg caggtgcagc aggtggcgca caacccgcgc ggtgaagtcg
     1021 gggtccgtga acagtcgctg cagcgggctt ttcgtggaag catcagtcgg cagtcgcgcg
     1081 ttgccacgca ggatctccgc ttcgcgcgtt ttgaatgcca tgccgatctc ccgcagttcg
     1141 gaggcaacgc ccgaacggtc ggcgacgttc ccgacaaaac cgcgtgccgc tcggctatgc
     1201 gccggcgact ggcatgctcg cgcgaattcc ggcctcgacg tccgcgtact tcagcgtcac
     1261 gcccaggtgc ttcagcatcc gttcgttgct cacccgacgc gactcattca ggaatgacaa
     1321 tcgctccggg ctgaacgtga gctgcgcttc ttccatcgag acctgcggcg gcgcgggcag
     1381 accggcgacc ttcgccacca tattgatgaa cgtcgtcgag ctcagcggtc gtccgtcggt
     1441 gatgttgtag acgccgcgcg cttccgaatt cgtgatcgcg cacgtgcagg cctcgacgag
     1501 gtcctcgacc tggatgcgat tgttgatgcc tgcttcgcgc tcgcgcacga ccggctcgta
     1561 gtgccgcagg cgctcgagcg gcaggcgatc gggcccgtag attccaggca cgcgcagcac
     1621 gacccggcgc acccggcgct cggtgcacca gactcgcgtc atttcttccg cgctgatgcg
     1681 tcgacgcgct cgatccgtgc ggggctgcac gacgctgctt tcgtccacga cagcgccggc
     1741 ggtgtcgccg tagacgccgg tcgtgctcat gtagacgaag gtgtgcggcg gcacggatgc
     1801 gaggtgcagg aagcggtcga gacgcagatc gctctcgccg agcacgggag gaggcgtgag
     1861 ataaacgatc gcctccaggt ccaggctctc cgggatcgtc gtgccggcgc ggacgcgatc
     1921 gagatcgatg gccagcgcac gcacgccgag acgctccagc tccgccgcgt gctctgccga
     1981 gcgcaccagg ccggtgacgt cgtaacgctc ttgcagacgc aacgccagcc gctggccgac
     2041 gtagccgcag ccggcaataa gcacttgacg gcgattcgct tgcattgact ccgcttcgat
     2101 tataatttaa atttcagata ttcctgaaac aacggtcacg tcagatgaaa ctcactccga
     2161 ccgtacaacg ctgcatcctg cactggggcg aaatggccgg ccgttggggc gtcagccgct
     2221 cggtcgcgca gatccacgcg ctgctgttcc tggcaccgca accgctcacg gcggacgaca
     2281 tcgctgaaac gctcgcggtg gcgcgctcca acgtcagcgt cagcctgaaa gagttgcagc
     2341 agtgggatct ggtgagcgtg acgcatgtgc tgggcgaccg gcgcgattac ttccaggccc
     2401 gcaaggatat ctgggaagtc ctggcgatga tcatggacgg gcgcaagaag c
//
LOCUS       DATANS010000251         4836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_433930, whole genome shotgun sequence.
ACCESSION   DATANS010000251 DATANS010000000
VERSION     DATANS010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433930"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VHT22_03525"
     CDS             <1..773
                     /locus_tag="VHT22_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3136653.1"
                     /translation="PHLQAAELDLTMPYKSPLLDQLQPWAKKQAEQSGYKAVGLYLGV
                     LGIGYNTELVAKKKLPPPACWKDLAKPIYAGDVQMANPNASGTAYTAIATLVQVFGEE
                     DAFKLLKDMHKNISSYSRQGVGPIKATARGENMVAVAFIHDVVTEAQGGFPVKSVAPC
                     EGTGYEIGSMSIIKGARNLENARKFYDWALTPAAQALAAQAKSYQLPSNVGSPVSPLS
                     PKPAEIKLIDYNFAKYGASAERKRLLERWEREVGSTPK"
     gene            770..1828
                     /locus_tag="VHT22_03530"
     CDS             770..1828
                     /locus_tag="VHT22_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3136654.1"
                     /translation="MRIVASGTRLLRRSLALAACSIAAAMLPAPAMAQGTLAMYCSSP
                     NTAWCQGMATGFEKLTGVKVAVTQKATGELFAQIKAERANPKGDIWWAGPGDAFLQAA
                     EEGLLETYRSPNLGQLHDWARREAELSQYRVAGVYGGILALGYNTEVFARKKLPVPKC
                     WKDLTDPVYKGEVMLSNPNSSGTSYMMLATLVQIFGEDEAFRYMKALNANVNQYARSG
                     IGPMAAVIRGESGIGSTVLHGVINEIMRGFPVKPILPCEGVGYEVGSMAIIKGARNLE
                     NAKKFFDWALTPEGQQIGLDIKEYAIPTNRAVPLPPQVPSLTDIKLINYDTAKYGSAA
                     ERKRLLERWEREINSAAK"
     gene            1845..4064
                     /locus_tag="VHT22_03535"
     CDS             1845..4064
                     /locus_tag="VHT22_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX3136655.1"
                     /translation="MSPQRKAIALWLTVGAVGFLAIPWYALQDSILSGAWLRNYSARE
                     NAPALVQVLRHGRIWLLAIGAMLLLAAPLLAPGIARRLRARALLVVGAAGFVILAAQG
                     FAIGPQGWGFDSLARIFGSLATGQYGMGWGATLAATSFAMLFALGLAESGYFNGDAFI
                     ASSVVAIALSVAIFTFFPVLTILASAFEDGSGALSATAFFNRLFTAKIWTLACLSGGG
                     RCGVAWNTLLLALLCAVGCTALGLAFALIVTRTSFRYKRMLRALSVLPIITPPFVIGL
                     GLILMFGRAGLINQWLEWGFGIEPSRWIYGLPGLVLAQIFSFTPIAFLVLIGVVEGVS
                     PSIEEAAQTLRASRWRTFVDVSLPLMRPGLANAFLISFIESIADFGNPIVLGGNFGVL
                     ATEVFFSVVGAQLDQGRAATLGILLLLFALVAFFAQRRVLGRKVYTALAGKGDAGLPT
                     PLPTGVRRACYAVALPWAALTIAIYAMAMLGGFVEQWGRNYTPTLKHYAKAFSIEWVN
                     HGILWSGTAWSSFWTTVELSAIAAPITAVLGILTAYILTRHRFAGRAAFEFGTMLSFA
                     IPGTVIGVSYILAFNVPPIEITGTALVLIVCNVFRNMPVGVRAGIASMAQIDRSLDEA
                     STTLGARGATTLKRVLLPLLKPAIIAALVYSFVRSMTTLSAIIFLVSAEYEWATTYII
                     NRVTNGDYGVAIAYCSVLIVLMLAVIWIIQRLVGERTLGRRTAGADAGVAQLGATAA"
     gene            4072..>4836
                     /locus_tag="VHT22_03540"
     CDS             4072..>4836
                     /locus_tag="VHT22_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3136656.1"
                     /translation="MKAAAIEFRQVSKLYGEVAAVRDISFTIAASTLVTLLGPSGCGK
                     TTTLRMIAGLELPTSGSILIGGEDVTRVPAAERDVSMVFQSYALFPHMSVIENVGYGV
                     AVAGRPKDEVQSRAKAAMATVGLTGFDARLPSELSGGQQQRVAVARALVLEPAVLLFD
                     EPLSNLDARLRRQMREEIRDLQQRLSLTVVYVTHDQSEAMAVSDRIIVMNKAAIAQEG
                     APRELYEQPRDVFVAGFMGDANRVRGTLARVDAALGE"
BASE COUNT          819 a         1549 c         1592 g          876 t
ORIGIN      
        1 accctcattt gcaggccgcc gagctggatc tcacaatgcc gtacaagtcg ccgctgctcg
       61 accagctgca gccctgggcc aagaagcaag ccgagcagtc gggctacaag gcagtcggtc
      121 tttacctcgg cgttctcggc atcggctaca acaccgagct ggtggcgaaa aagaagctgc
      181 cgccgccggc ctgctggaag gacctcgcca agcctattta cgcgggcgat gtgcagatgg
      241 ccaatccgaa tgcgtcggga accgcgtaca cggccatcgc tacgctggtt caggtgtttg
      301 gtgaagaaga tgcgttcaag ctgctgaagg acatgcacaa gaacatcagc tcgtactcac
      361 gccagggcgt cggaccgatc aaggcgacgg cgcgcggcga aaacatggtt gcggtggcat
      421 tcattcacga cgtcgtcacc gaagcgcagg gtggattccc ggtgaaaagc gtcgcgcctt
      481 gcgaaggcac cggctacgag atcggctcga tgtcgatcat caagggggcg cgcaatctgg
      541 aaaacgcgcg caagttctac gactgggcgc tgacgcctgc cgcccaggct cttgccgcgc
      601 aagccaagtc gtaccagctg ccgtcgaacg taggctcgcc tgtctcgccg ctttcgccca
      661 agccggccga gatcaagctg atcgactaca acttcgccaa gtacggggcc tccgccgagc
      721 gcaagcgcct gctcgagcgc tgggagcgcg aagtcggcag cacgccaaaa tgaggatcgt
      781 cgcttccgga acacgcctgc tgcgccgatc gctggcgctg gccgcctgca gcatcgccgc
      841 ggcgatgcta cccgcccccg caatggccca gggcacgctg gcgatgtact gcagctcgcc
      901 gaataccgcc tggtgccagg gcatggcgac cggattcgaa aagctcacag gcgtcaaggt
      961 cgcggtcacg caaaaggcga ccggcgagtt gttcgcgcag atcaaggcgg agcgtgccaa
     1021 tccgaaaggc gacatctggt gggccgggcc gggcgacgcg tttctgcagg cagccgagga
     1081 aggactgctc gagacttacc ggtcgccgaa cctcggacaa ctgcacgact gggcgcgccg
     1141 cgaagcggag ttgtcgcaat accgcgttgc cggcgtttac ggcggcatcc tggcgcttgg
     1201 ctataacacc gaggtcttcg cccggaagaa actgccagtg ccaaaatgct ggaaggacct
     1261 gaccgacccc gtctacaagg gtgaagtcat gctgtcgaac ccgaattcct cgggcacgtc
     1321 ttacatgatg ctggcgacgc tggtccagat cttcggcgaa gacgaagcgt ttcgctacat
     1381 gaaggcgctc aatgccaacg tcaatcaata cgctcgttcg ggcatcggcc cgatggccgc
     1441 ggtgatccgc ggcgaaagcg gcatcggcag cacggtgctg cacggcgtga tcaacgaaat
     1501 catgcgcggc tttccggtca agccgatact gccatgcgaa ggggtcggct acgaggtcgg
     1561 ctcgatggcg atcatcaagg gcgcgcgcaa cctggaaaac gccaagaaat tcttcgactg
     1621 ggcgctgacg ccggaagggc agcagatcgg gctcgacatc aaggagtacg cgatacccac
     1681 caaccgcgcc gtcccactgc ctccgcaggt gccgagtctt accgacatca agctgatcaa
     1741 ctacgacacc gccaagtacg gttcggcggc ggagcgcaag cgcctgctcg agcgctggga
     1801 acgagagatc aactcggccg cgaaatgacg ccgctcttcg gagcatgagt ccgcagcgca
     1861 aggcgattgc gctctggctc accgtcggag cggtgggctt cctggcgatt ccctggtatg
     1921 cgctgcagga ttcgatcctt tccggcgcgt ggctccgcaa ttattcggcc agggaaaacg
     1981 cgccggcgct ggtgcaggta ttgcgccacg ggcggatctg gctgctcgcg atcggggcga
     2041 tgttgctgct tgcggccccg ctgctcgcac ccggcatcgc gcgcaggctt cgcgcgcgcg
     2101 ccctgctggt cgtcggcgct gccggcttcg tcattctcgc cgcccagggc ttcgccatcg
     2161 gcccgcaagg ctggggtttc gattcgctcg cccggatatt cgggtcgctt gccaccggcc
     2221 agtacggcat gggctggggc gcgacgctcg ccgcgacgtc gttcgcgatg ctgttcgcgc
     2281 tcgggctcgc tgagagcggc tacttcaacg gcgatgcgtt catcgcaagc agcgtcgtcg
     2341 cgatcgcgct ttcggtggcg atcttcactt ttttcccggt cctcacgatc ctggcgagcg
     2401 cattcgagga tggcagcggc gcgctttcgg cgacggcgtt cttcaaccgc ctgttcaccg
     2461 ccaagatctg gaccctggca tgcctgtcgg gcggcgggcg ctgcggcgtg gcttggaaca
     2521 cgctgctact tgcgctgctg tgcgcggtcg gctgtacggc gctcggactt gccttcgcgc
     2581 tgatcgtcac gcgcacgagc ttccgctaca agcgaatgct gcgtgcgctg tcggtgctgc
     2641 ccattatcac gccgccattc gtgatcgggc tcggattgat cctgatgttc ggccgcgcgg
     2701 ggctgatcaa tcaatggctc gagtgggggt tcggcatcga gccatcgcgc tggatctacg
     2761 ggcttccggg gctggtgctc gcgcagatct tttcgttcac gccgatcgct ttcctggtgc
     2821 tgatcggcgt ggtcgaaggc gtatcgccga gcatcgagga ggcggcgcag accttgcgcg
     2881 ccagccgctg gcgaactttc gtcgacgtat cgctgccgct gatgcggccg ggactggcga
     2941 acgccttcct gatcagcttc atagaatcga tcgcggattt cggcaacccg atcgtcctcg
     3001 gcggcaactt cggcgtgctg gcgaccgagg tcttcttttc ggtggtcggc gcgcagctcg
     3061 atcaggggcg cgccgcgacc ctgggcatac tgctgctgct gttcgcgctg gtcgcctttt
     3121 tcgcgcagcg tcgcgtgctt gggcgcaagg tctataccgc gctggccggc aagggcgatg
     3181 cgggcctgcc gacaccgctg ccgaccggcg tgcgccgcgc ctgctatgcg gtggcgctgc
     3241 cgtgggcagc gctgaccatt gcgatctacg cgatggcgat gcttggcggc tttgtcgagc
     3301 aatggggccg caattacacg ccgaccctga agcactacgc gaaggcgttc tcgatcgagt
     3361 gggtcaacca cggcatcctc tggtccggca ccgcctggag ctcgttctgg accaccgtcg
     3421 agctctcggc gatcgccgcg ccgatcacgg cggtcctcgg gatcctgacc gcgtacatac
     3481 tgacccggca tcgattcgcc ggccgggcgg ctttcgagtt cggcacgatg ctatcgttcg
     3541 cgattcctgg cacggtgatc ggcgtctcgt acatcctcgc cttcaatgtc ccgccgatcg
     3601 aaatcaccgg caccgcgctg gtgctgatcg tctgcaacgt ctttcgcaac atgccggtag
     3661 gcgtgcgcgc cggcattgcc tcgatggcgc agatcgaccg aagcctcgac gaggcgtcga
     3721 caacgctggg cgcgcgcggc gcgacgacgc tcaagcgcgt gctgctgccg ctgctcaagc
     3781 cggcgatcat cgcggcgctc gtgtacagct tcgtgcgctc gatgacgacc ttgtcggcga
     3841 tcatcttcct ggtgtcggcg gaatacgaat gggcgacgac ctacatcatc aaccgggtca
     3901 ccaacggcga ttacggcgtt gctatcgcct actgctcggt gctgatcgtg ctgatgctcg
     3961 cggtgatctg gatcatccag cggcttgtcg gcgaacgcac gctcggccgg cgcacggccg
     4021 gggcggacgc cggcgtggca caattgggcg cgacagccgc gtgagtgaaa aatgaaagcc
     4081 gcagcgatcg aatttcggca ggtcagcaag ttgtacggcg aagtcgccgc cgtgcgggac
     4141 atctcgttca cgatcgcggc cagtacgctg gttacgctgc tcggcccttc gggctgcggc
     4201 aagaccacga cgttgcgcat gatcgccgga ctcgaattac cgacgtcagg cagcatcctg
     4261 atcggcggtg aggatgtcac gcgcgttccc gcggccgagc gcgacgtgtc gatggtattc
     4321 cagagctacg cgctgtttcc gcatatgagc gtcatcgaga acgtcggcta cggggtagcc
     4381 gtggccggac ggccgaagga cgaagtgcaa agccgggcca aggccgcgat ggcaacggtc
     4441 ggcctcaccg gtttcgatgc gcgactgcca tccgagctct cgggcgggca gcagcagcgc
     4501 gtcgccgtgg cccgcgcact ggtgctcgag ccggctgtgc tgctgttcga cgagcccctg
     4561 tcgaatctcg acgccaggtt gcggcggcag atgcgcgagg aaatccgcga cctgcagcag
     4621 cggctgtcac tcacggtcgt ctacgttacc cacgaccagt cggaggcaat ggccgtatcg
     4681 gatcgcatca tcgtgatgaa caaagccgcc atcgcccagg aaggcgcgcc gcgcgagctc
     4741 tacgagcagc cgcgcgacgt ctttgtcgcc ggcttcatgg gcgacgccaa tcgcgtacgc
     4801 ggcaccctgg cgcgtgtgga cgccgcgctg ggcgag
//
LOCUS       DATANS010000252         2792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_559696, whole genome shotgun sequence.
ACCESSION   DATANS010000252 DATANS010000000
VERSION     DATANS010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559696"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="VHT22_03545"
     CDS             complement(<1..1250)
                     /locus_tag="VHT22_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006767295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX3136657.1"
                     /translation="MSLDAVLLSPLQFVWVVALHILLPAFTVGLAAYIAVLEGLHLTT
                     ARAVYLRLSKFWLKVFAVSFGMGVVSGIVMPFQFGTNWSRFSDATGDIGGPLMAYEGL
                     MAFFLEAGFLGVLLFGRARVPPWVHFFAALMVALGTLFSTFWILAFNSWMQTPRGYTI
                     VDGRFFAEDWFAVIFNPSFPYRLTHTAVAFFITTALVVAGVASYHLRADRFREEGTIM
                     LKMAFGLLTLLVPLQMLLGDLHGLNTRDHQPAKLAAMEANWETQSRMPLLLFAWPDER
                     AEANRFEVAIPALGSVILTHDADGVVQGLKEWKPEDRPPVAIPFFSFRLMVGIGLAML
                     ALTVCSWWVWARGELDRSRWFHKLCEIASPLGFVAVIAGWVTTEVGRQPWTVYGLMRT
                     KDSVTPSLAGSDVLASLIVYVVAY"
     gene            1379..2116
                     /locus_tag="VHT22_03550"
     CDS             1379..2116
                     /locus_tag="VHT22_03550"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEX3136658.1"
                     /translation="MSKSKIALIAVHAFASVLLAAAVPAALAQSDQTGKAESTTPRAK
                     PTPQTLVDAADRTFGNFMRDSQMAWLHNNIRRAKAVLIAPEVAKAGFIFGGSGGRAVL
                     LVRNAKTGRWMGPAFYTLTTASVGFQAGVSVSEMMTLVMTDKGLNSLLSNSFKMGADA
                     SVAAGPVGAGAQADVVTDLVTYSHAKGIYGGINLDGTVVAISDEWNEAYYGKKGIHAP
                     DILVRGAVHAKGADRLASSVARAAGKK"
     gene            2215..>2792
                     /locus_tag="VHT22_03555"
     CDS             2215..>2792
                     /locus_tag="VHT22_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3136659.1"
                     /translation="MDAAPVPRLELSGITKRFPSVVANDGIDLTVMPGEIHAVLGENG
                     AGKSTLIKIVYGVVWPDAGTIRWNGEVLVIDSPAKARALGIGMVFQQFSLFETLTVAE
                     NIALALDKSWGGRELDTRIRETSAQYGLALDPRRHIHTLSVGERQRVEIVRCLLQTPE
                     LLIMDEPTSVLTPAAIGTLFGTLRRLADEGCS"
BASE COUNT          559 a          895 c          905 g          433 t
ORIGIN      
        1 atgtaggcga cgacgtacac gatcagcgac gcaaggacgt cgctgccggc cagcgatggc
       61 gtgaccgaat ccttcgtccg catgagtccg taaaccgtcc atggctgccg gcccacttca
      121 gtcgtcaccc agccggcgat caccgcgacg aatcccagcg gcgacgctat ctcgcatagc
      181 ttgtggaacc agcgcgaacg atcgagctcg ccgcgcgccc agacccacca gctgcacacg
      241 gtcagcgcca gcatcgcgag gccgatgcca accatcagcc ggaacgaaaa gaacggtatc
      301 gcgacgggcg gacggtcttc tggcttccac tccttgaggc cttggacgac gccgtctgca
      361 tcgtgcgtca ggatcacgga gcccagcgcc gggatggcga cttcgaagcg attggcctcg
      421 gcgcgctcgt cgggccacgc gaacagcaac agcggcatgc gactttgcgt ttcccagttg
      481 gcttccatcg ccgcgagctt cgccggctga tggtcgcgcg tgttgaggcc gtgcagatct
      541 ccgagcagca tctgcagcgg caccagcagc gtcagcaggc cgaacgccat cttgagcatg
      601 atcgtccctt cctcgcggaa gcggtcggcg cgcaggtgat aggaggcgac gccggcgacg
      661 accagcgcag tggtgatgaa aaatgcgacc gcggtgtgcg tgaggcggta cggaaacgat
      721 ggattgaaaa tcaccgcaaa ccagtcttcg gcgaaaaacc ggccatcgac aatggtgtat
      781 cccctcggcg tctgcatcca gctgttgaat gcgaggatcc agaaggtcga gaacagggta
      841 ccgagcgcga ccatgagcgc agcgaagaag tgcacccacg gtgggacgcg ggcgcgtccg
      901 aacagcagca cgccgagaaa tcccgcctcg aggaagaacg ccatcagccc ttcgtatgcc
      961 atcagcggtc cgccgatgtc gccggtcgcg tcggaaaagc ggctccagtt ggtgccaaac
     1021 tggaacggca tgacgatgcc agaaacgacc cccatgccga acgagacggc gaacaccttg
     1081 agccagaatt tggacagtcg cagatagacg gcgcgtgccg tcgtcaggtg cagaccctcg
     1141 agcacggcaa tgtatgcggc aagcccgacg gtgaatgcgg gcagcaggat gtgcagcgcg
     1201 acgacccaga cgaactgcag cggcgagagc aagaccgcgt cgagtgacat tgctttctca
     1261 gcccggacgg ttgcgagctg ccactatagc attcggccgc aaataccatc cgcgggcgcg
     1321 gcggaatata atgccgacgc aggtccgatg aaccccatgc atccggaggg agcaagccat
     1381 gtcgaagtca aaaatagcgc tcatcgcagt ccatgcattc gcgtcggtct tgctggccgc
     1441 ggcggtgccc gcggcgctcg cgcaatcgga tcagaccggc aaggccgaga gcacgacccc
     1501 ccgtgcaaag ccgacgcccc aaacgctggt cgatgccgcc gatcgaacgt tcggcaattt
     1561 catgcgcgac tcgcagatgg cctggctgca caacaacatc cgtcgcgcga aggcggtatt
     1621 gatcgcgccg gaagtcgcca aggcaggatt catcttcggc ggctcgggcg gacgcgcggt
     1681 gcttctggtg cgcaatgcca agaccggcag gtggatgggc ccggcgttct acacgctgac
     1741 gactgcgagc gtcggcttcc aggctggggt gtcggtctcg gaaatgatga ccttggtcat
     1801 gaccgacaag ggtctcaatt cactgctgtc caactcgttc aagatgggtg ccgatgcaag
     1861 cgttgcggcg gggccggtcg gcgccggcgc ccaggcggac gtagtcaccg acctcgtcac
     1921 ctacagccac gcgaagggca tctacggcgg catcaacctg gacggaaccg tggtcgcgat
     1981 ttccgatgag tggaacgaag cgtattacgg caagaagggg atacatgcgc cggacatcct
     2041 ggttcgcggt gccgtgcacg ccaagggcgc ggaccggctg gcctccagcg tagcgcgcgc
     2101 cgccggcaag aagtgaacga ccggcggcgc ctggccatcg tggtcgagcg ccgccgcatg
     2161 cggtgcgcgg aacatccgcg caccgcctcg atggcgactc tgcgcagctg acgcatggac
     2221 gcggcgccgg ttccacgtct ggagctttcc ggcatcacca agcgtttccc ttcggtcgtc
     2281 gccaacgacg gcatcgatct cacggtcatg cccggcgaga tccacgcagt gctcggcgaa
     2341 aacggcgccg gcaaatcgac cctgatcaag atcgtctacg gcgtcgtctg gcccgacgcc
     2401 ggcacgatac gctggaacgg cgaagtactc gtcatcgaca gcccggcgaa ggcacgcgcg
     2461 ctgggcatcg gcatggtgtt ccagcagttc tcgctgttcg agacgctcac cgtcgccgaa
     2521 aacatcgcac tggcgctcga caagagttgg gggggacgag aactcgatac gcggattcgc
     2581 gagacttccg cacagtacgg cctggcgctc gatccgcgcc gccacatcca cacgctgtcg
     2641 gtaggcgagc gccagcgcgt cgagatcgtg cgctgccttc tgcagacgcc cgagctgctg
     2701 atcatggacg agcccacgtc ggtgttgacg ccggcggcga tcggcacact gttcggcacg
     2761 cttcggcgcc tggccgacga aggctgctcg at
//
LOCUS       DATANS010000253         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_55066, whole genome shotgun sequence.
ACCESSION   DATANS010000253 DATANS010000000
VERSION     DATANS010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55066"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VHT22_03560"
     CDS             <1..648
                     /locus_tag="VHT22_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010118891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX3136660.1"
                     /translation="PFVYRELNYNALHPPTWNPALEITAILLAAGAGSRFGGDKLLHP
                     LPDGVAIGAHAARNLVAAGLDVVAVVKQGDFPLADVLEQEGCNVTMFADYARGMGASL
                     AHGVALRRSADGWIIALADMPRIRSSTIAMIAKELTAGRELVAPAYKGQRGHPVGLGK
                     RFGAQLAALGGDAGARDIVAANPSELLLIECDDPGVLHDIDRREDLAPPAAGDRA"
     gene            641..1618
                     /locus_tag="VHT22_03565"
     CDS             641..1618
                     /locus_tag="VHT22_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX3136661.1"
                     /translation="MRKVPALIWMAVALLLCAGVSTAQNYPSRYIRMIVPYVPGGGTD
                     TLTRTIAPRLGETLGQQIIIDNRVGGSSAVGTEIAAKAPHDGYTLLMVDTSFTTNPAL
                     YGKLPFDPVKDFAPVSLVASAPVILVVHPSVPIRNVKEFVALAKARPGELNFASGAPG
                     SSTHLGGELLKSVAKVDVVNIPYKGTGQAITDVLGGQVGMMFAGISTVRQHVRAGKLR
                     AIAVTGEKRAAAMPEVPTFVESGLVGVDSGTYWGCLAPAATPRDIVNKVSAALHSVVE
                     SADVKPRLEERGYTVIGSAPERFGENIRSEIAKWAKVIKTAGIKVEAQQ"
     gene            1615..>1847
                     /locus_tag="VHT22_03570"
     CDS             1615..>1847
                     /locus_tag="VHT22_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3136662.1"
                     /translation="MTGRLQGRSAFITGAGAGIARVAALLFAREGANVAVIEINEEKG
                     AATAQAVRDAGGDAVFIHTDITQEDSVKSAVTEA"
BASE COUNT          329 a          585 c          637 g          296 t
ORIGIN      
        1 ccgttcgtat atcgcgaact taattataat gctttacacc cgcctacctg gaatcccgct
       61 ctggaaatca ctgccatcct tcttgccgcc ggcgccggct cgcgcttcgg cggcgacaag
      121 ctgctgcacc cgctgcccga cggcgtcgcg ataggcgcgc acgcggcgcg caacctcgtc
      181 gcggcagggc tggacgtcgt ggccgtcgtc aagcagggcg attttccgct ggccgacgtg
      241 ctcgagcagg aaggctgcaa cgtgacgatg ttcgcggact acgcgcgcgg catgggcgcg
      301 agcctcgcgc atggcgtcgc gctaaggcgc agcgcggacg gctggatcat cgcgctcgcc
      361 gacatgccgc gcatcaggag ttccaccatt gcgatgattg cgaaggagct caccgcgggc
      421 cgggaactcg ttgcgccggc ctataagggg caacgcggac acccggttgg cctgggtaag
      481 cgtttcggcg cgcagctcgc ggcgctcggc ggggacgccg gtgcgcgcga catcgtcgct
      541 gccaacccga gcgagctgct gctaatcgaa tgcgacgatc ccggcgtgct gcatgacatt
      601 gacaggcgtg aggatctcgc gccacccgcg gcgggcgatc gtgcgtaagg tcccggcgct
      661 gatatggatg gcggtggcct tgctgctgtg cgcgggcgtt tccacggcgc aaaattatcc
      721 ttcccgctac atccgcatga tcgtgccgta cgtgccgggc ggcggcaccg acacgctgac
      781 gcgcacgatc gctcccaggc tcggcgaaac gctgggccag caaatcatca tcgataatcg
      841 cgtcggcggc agcagtgccg tcggcaccga gatcgccgcg aaggcgccgc acgacggcta
      901 tacattgctg atggtcgata cgtcgttcac gaccaacccc gcgctctacg gcaagctgcc
      961 gttcgatccg gtgaaggatt tcgcgccagt gtcgctggtg gcgtcggcgc ccgtgattct
     1021 ggtcgtgcac ccgtcggtgc ccatccgcaa cgtgaaggag tttgtcgcgc tcgcgaaggc
     1081 gcgcccgggc gagctcaatt tcgcgtcggg ggcgccgggc agctcgacgc atctcggcgg
     1141 cgaactgctg aaatccgtcg cgaaagtcga tgtcgtcaac attccgtaca aaggcactgg
     1201 gcaggcgatc accgacgtgc tgggcgggca ggtcggcatg atgttcgccg gcatcagcac
     1261 ggtgcggcaa cacgtgcgcg cgggcaagct gcgcgcaatc gcagtgaccg gggagaaacg
     1321 cgccgcggcg atgcccgagg tgccgacgtt cgtggagtcg gggctcgtgg gcgtcgattc
     1381 cggcacgtac tggggctgtc tcgcgcccgc cgcgacaccc agggacatcg ttaataaagt
     1441 cagcgcagct ctgcacagcg tggtcgaatc ggccgacgtc aagccgcggc tcgaggagcg
     1501 cggttatacc gtgatcggca gcgcgcccga gcggttcggc gagaatatcc gcagtgaaat
     1561 cgccaaatgg gcgaaagtca tcaaaaccgc aggcatcaaa gtcgaggcgc aacaatgacg
     1621 ggcagactgc aaggcagaag tgcgttcatc accggtgccg gcgcgggcat tgcgcgcgtc
     1681 gcggcgctgc tcttcgcgcg cgaaggggcc aacgtggccg tcatcgagat caacgaggag
     1741 aaaggcgcgg cgaccgcgca ggcggtgcgc gatgccggcg gcgacgcggt gttcatacat
     1801 accgatatca cgcaggaaga cagcgtgaag agcgccgtca cggaagc
//
LOCUS       DATANS010000254         1700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_643764, whole genome shotgun sequence.
ACCESSION   DATANS010000254 DATANS010000000
VERSION     DATANS010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643764"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1173)
                     /locus_tag="VHT22_03575"
     CDS             complement(172..1173)
                     /locus_tag="VHT22_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136663.1"
                     /translation="MVSAQTVSAGAAEAPAASAAPSPPPSLDRAIEDRILALDPLHIS
                     AAEVHDVLKRAPAPRIIGIQGSFPLITMEPFAQYLIAMGYPEERLRDPRDGSMSRNSF
                     GDSEQLAGELAWYYESDGMMPMLIGHSQGGMLAIKVLYELNGSFHQTIRVWNPLTGQP
                     EQRTSIVDPLTGLERPVVGLKVPYVAALATGKLPRFLLGQWAMMGKLRAIPDTVEEFT
                     GFSIEWDMIAGTPPGSEEYHADGIAQVRNVELPATTSHIRMPRTRYLTRNAATRAWID
                     DYDPAAPQPALPEGPDVDSANIVHAADIWYSIKKHWCIEAQRLIRARRAAIKPGGAE"
     gene            complement(1313..>1700)
                     /locus_tag="VHT22_03580"
     CDS             complement(1313..>1700)
                     /locus_tag="VHT22_03580"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX3136664.1"
                     /translation="NVPAYVALRWVAALCVVMCLALTVKCWRRMGSDWRMAVSDTQKS
                     ALITDGLFARVRHPIYALQMLLMLCSVLIVPTAPMIAVAIVHLGLMNLKARNEERHLL
                     KVHGARYEAYLERTGRFFPRLGGRDS"
BASE COUNT          275 a          572 c          559 g          294 t
ORIGIN      
        1 ggccgtcgag cataagcccg gtgaggccga ggccctcgag ctggcggcga acgcacgctt
       61 cgagccgcga cacgtcgagc ggttgcggca gcgtgaccac atgcaccgca ttgtacggat
      121 gcatttcgcg ccagcggacc atcgccgcct gaaaaaggtt gagccggccc ttcactcggc
      181 gccccccggc ttgattgcgg cgcggcgcgc ccggatcaac cgctgcgcct cgatgcacca
      241 gtgctttttg atgctgtacc agatgtccgc cgcgtgcacg atgttggcgc tgtcgacgtc
      301 cggtccctcg ggcagcgcgg gctgcggcgc cgcggggtcg taatcgtcga tccaggcgcg
      361 cgtcgccgca ttgcgcgtca gataccgcgt gcgcggcatg cggatatggc tggtcgtggc
      421 gggcagctcg acgttgcgca cctgcgctat gccgtcggcg tggtattcct cgctgcccgg
      481 cggcgtgccg gcgatcatgt cccactcgat cgagaacccg gtgaattctt cgaccgtgtc
      541 ggggatcgcg cggagcttcc ccatcatcgc ccactgtccg aggagaaaac gaggtagctt
      601 gccggtggcg agcgcggcga cgtacggaac cttgagtccg accaccgggc gctcgaggcc
      661 ggtcagcgga tcgactatgc tggtgcgttg ctccggctgg ccggtcagcg gattccacac
      721 ccgaatcgtc tgatggaacg atccgttgag ctcgtagagc accttgatcg ccagcatgcc
      781 gccctggctg tggccgatca gcatcggcat catgccgtcg gattcgtaat accaggccag
      841 ctcgccggcc agttgctcgc tgtcgccaaa gctgttgcgc gacatgctgc cgtcgcgcgg
      901 atcgcgcagc cgctcttccg gatatcccat cgcgatcagg tattgcgcga acggttccat
      961 cgtgatcagc ggaaagcttc cctggatgcc gatgattcgc ggcgccggcg cgcgcttgag
     1021 cacgtcgtga acttctgcgg cgctgatgtg cagcggatcg agcgcaagga tgcggtcctc
     1081 gatggcgcga tcgagcgatg gcggcgggct tggcgcggcg ctcgcggcag gggcctcggc
     1141 cgctccggcg gataccgtct gcgccgagac cattgcagcc agcagcccaa gcgccgcgcc
     1201 ggcacacaga ccctgcagtg cccggcgccg gcgcaagccg ccgcggttcc cgcttgtcgt
     1261 gcccggcatc acgattcgag catcgtatcg atcggatcgc gctcgacagg ctctagctgt
     1321 cccggccgcc cagccgcgga aaaaagcggc cggtgcgttc gaggtaggcc tcgtagcggg
     1381 cgccatgcac cttgagcaga tgccgctcct cgtttcgcgc cttgaggttc atcagaccga
     1441 ggtgcacgat cgcaacggcg atcatcggcg ccgtcggcac gatcagcacc gagcagagca
     1501 tcaacagcat ctgcagggca tagatcggat gccggacgcg cgcgaacaag ccgtcggtga
     1561 tcagcgcgct cttttgcgta tcgctgactg ccatccgcca gtcgctgccc atcctccgcc
     1621 agcacttgac ggtaagcgcc aggcacatca ccacgcaaag cgctgccacc cagcgaagtg
     1681 cgacatacgc gggaacattt
//
LOCUS       DATANS010000255         2967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_182065, whole genome shotgun sequence.
ACCESSION   DATANS010000255 DATANS010000000
VERSION     DATANS010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182065"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VHT22_03585"
     CDS             <1..276
                     /locus_tag="VHT22_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX3136665.1"
                     /translation="IGNPPPPLVLPEVVKIDIDDRGAIYWNGEVVPDRATLEEKMTAS
                     ASQQVQPEVHLRPDKESKYSVAAAVMASAQRLGLTKIGIVGSEQFIE"
     gene            318..962
                     /locus_tag="VHT22_03590"
     CDS             318..962
                     /locus_tag="VHT22_03590"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX3136666.1"
                     /translation="MDFARQQRDPTRHLIGISIVILVHVVVIYALVTGLARKAVEVIK
                     KPLTATIIEEIKLPPPPPPPPPKKIEIPKAPPPPEQPYVPPPDIPPPVAPSEPVIAAP
                     SITPPPEPHVIAPPPPAPPPPAPKPAIRRGLVPIDKVDPVFPERARRDGVESGKVVAR
                     LQVDEKGSVTEVRIVSANPPRVFDREVIRALSMWRFKPEGDKYEAEVEVNFRLE"
     gene            complement(1112..1465)
                     /locus_tag="VHT22_03595"
     CDS             complement(1112..1465)
                     /locus_tag="VHT22_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136667.1"
                     /translation="MNTQDIDTADWLDAALKEAGREHRAGYVADDGFTARVVGRLPAA
                     PTLPAWRRPVIILLWLLAGAGAFVALPGAFDDAFRGAVALLVGRPLGLADIALLLALF
                     GAAAWTSLVYVARAN"
     gene            complement(1462..2028)
                     /locus_tag="VHT22_03600"
     CDS             complement(1462..2028)
                     /locus_tag="VHT22_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3136668.1"
                     /translation="MSSTPVSDAQLIARAVVQDDRHAFGELVRRHQSAVRSTLRRLTG
                     GNIAQADDLAQETFLLAYRNLKSFRQEAKFSTWLYRIAYNVFLADARKIKELPMPEDA
                     DGEPVAAADATAPSMSRQAALSVDLERAMRVLSEPERAAIVQCYHNDLSHEEAAQVLG
                     CPVGTVKTHILRAKQKLKARLGAWSPQA"
     gene            complement(2062..2835)
                     /locus_tag="VHT22_03605"
     CDS             complement(2062..2835)
                     /locus_tag="VHT22_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136669.1"
                     /translation="MRSQFVRFRVMPMAFGALLAAMLCLAAAPGVAADAPAKGGAAVQ
                     KSPPAPPAPPPAPAAAPAAPSSAATDNSATADEPDDPFPFGRHRGHIQFDDFDSFKQA
                     MDVAPWIVGVVFLVLGSIFLTPIILLVGIIWYKLRKTRLQNDAVLRLAERGVMPSAQA
                     VDAVMAGTPPAQAAPAAIAAAVPQAVVTRRRAVWSDLRKGVLLIAVGLALVLYSITDA
                     GEPNWLGLVLLFVGAGYVALWWLEDRHLERRVAGGDGNG"
BASE COUNT          537 a          997 c          971 g          462 t
ORIGIN      
        1 atcggcaatc cgccgccgcc gctggttctg cccgaggtcg tcaagatcga catcgacgac
       61 cgcggcgcca tctactggaa cggcgaagtc gtgcccgacc gcgctacgct cgaggagaag
      121 atgacggctt cggcctcgca acaggtccaa cccgaagtcc acctgcggcc ggacaaggag
      181 tcgaaatatt ctgtcgccgc ggcggtgatg gcatcggcgc agcgattggg gctgaccaag
      241 atcggcatcg tcggcagcga gcagttcatc gagtagccgc gccgcgaact gcggcaggcg
      301 gctggagtca tagccacatg gatttcgcac gacaacagcg ggacccgacc cggcatctga
      361 tcgggataag catcgtcatc ctggtgcacg tggtggtgat ttatgcactg gtgaccggcc
      421 tcgcgcgcaa ggctgtggag gtgatcaaga agccgctgac ggcgacgatc atcgaggaga
      481 tcaagctccc gccaccgccg cccccgccgc cgccgaagaa gatcgaaata cccaaggcgc
      541 cgccgccgcc cgagcagccg tacgttccgc cccccgatat tccgccgccg gtcgcacctt
      601 cggaaccggt gatcgccgcg ccgtcgatta caccgccgcc ggagccgcac gtgatcgcgc
      661 ccccgccgcc ggcaccgccg ccgccggcgc ccaagcctgc aattcgccgg ggcctggtgc
      721 cgatcgacaa agtggatccg gtctttccgg aaagagcgcg ccgcgacggc gtcgagagcg
      781 gcaaggtcgt cgcgcgcctg caagtcgatg agaagggctc ggtcaccgag gtcaggatcg
      841 tcagcgccaa tcctccgcgc gtgttcgatc gcgaagtcat ccgcgcgctt tcgatgtggc
      901 gattcaagcc cgaaggcgac aagtacgaag ccgaagtcga agtcaacttc cgattggaat
      961 aacgcgggcc ggcctgctcg gccggaaaac aaaatggccg cctcgaaagg cggccatttt
     1021 tttgccggcg cgcaagctgc cgcgcgatgg aaattcggcg ctcagttgcc ctcagttgcc
     1081 ttcaggcgac ctcagtttgc ctcagtttgc ctcagtttgc tcttgccacg tagaccagcg
     1141 aggtccacgc cgcagcaccg aacagagcga gcagcagcgc gatgtcggcc aagcccagcg
     1201 gacgtccgac aagcaacgcc acagcgccgc ggaatgcgtc gtcgaaggcg ccgggcagag
     1261 cgacaaacgc accggcgccg gccagcagcc acagcaaaat gatcaccggc cgccgccacg
     1321 caggcagcgt cggcgccgca ggcagacggc cgactacgcg cgcggtaaaa ccgtcatcgg
     1381 cgacataccc agcgcgatgc tcgcgtcccg cttctttgag cgcggcgtcg agccagtcgg
     1441 cggtgtcgat gtcctgtgta ttcacgcttg cgggctccat gcacccagtc gcgccttgag
     1501 cttctgcttg gcgcgcagga tgtgcgtttt caccgtgccg acggggcagc ccagcacctg
     1561 cgcggcttcc tcgtgcgaca ggtcattatg atagcactgc acgatcgccg ctcgctccgg
     1621 ctctgaaagc acccgcatcg cgcgttccag atcgacgctc agggccgcct gccgcgacat
     1681 gctcggcgcc gtggcatccg ctgcggcgac cggctcgccg tcggcgtcct cgggcatcgg
     1741 cagctccttg atctttcgcg cgtcggccag aaaaacgttg taggcgatcc gatacagcca
     1801 ggtcgagaac ttggcttcct ggcgaaacga cttcagattg cggtaggcga gcaagaaagt
     1861 ctcctgagcc agatcgtcgg cttgcgcgat gttgcccccg gtcagcctgc gcagggtcga
     1921 ccgcacggcg gactggtgtc gccgcaccag ctcgccgaaa gcatggcgat cgtcctggac
     1981 gactgcgcgg gcgatcagct gcgcgtcgct gaccggggtg gacgacatcg agattgcctg
     2041 ccggcgtagg cacgtctcct ttcaaccgtt gccgtcgccg ccggcgacgc gacgctccag
     2101 atgccgatcc tccagccacc acaacgcgac atagcctgcg ccgacgaaaa gcagcaccag
     2161 acccagccag ttcggctcac cggcgtcggt gatcgaatac agcaccagcg ccagcccgac
     2221 cgcaatcagg agtacgcctt tgcgcaggtc cgaccacacg gcgcgccgtc gtgtcacgac
     2281 cgcctgggga acggcggcgg cgatcgccgc cggtgccgct tgcgccggcg gtgttccggc
     2341 catcaccgcg tcgaccgcct gggccgaggg catgacgcct ctttcggcga gtctcagcac
     2401 ggcatcgttt tgcaggcgcg ttttgcgcag cttgtaccag atgatcccga ccagcaggat
     2461 gataggcgtc agaaagatcg atccgagcac caggaagacg acgccgacga tccatggcgc
     2521 aacgtccatc gcctgcttga acgagtcgaa gtcgtcgaac tgaatgtggc cgcggtggcg
     2581 gccgaacgga aacgggtcgt cgggttcgtc ggcggtcgcg ctattgtcgg ttgcggccga
     2641 cgatggggcg gcgggcgctg ccgcgggggc gggcgggggc gcgggcggcg cgggaggtga
     2701 tttctgcacc gcggcgccac ctttggctgg cgcatcggcg gccacaccgg gggcggcggc
     2761 gaggcacagc atcgcggcca gcagcgcgcc gaacgccatc ggcatgacac gaaaacggac
     2821 aaactggctg cgcatacgca ctcctgataa atcgaggttt atggccttag atgccgcgag
     2881 atggcacggc ggattcaaca tccggaaggg gtccggcccc cctccggcgg gggtggacct
     2941 gcatgctatc attgcccgga ttttcgg
//
LOCUS       DATANS010000256         3533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_97257, whole genome shotgun sequence.
ACCESSION   DATANS010000256 DATANS010000000
VERSION     DATANS010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97257"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2349)
                     /locus_tag="VHT22_03610"
     CDS             complement(<1..2349)
                     /locus_tag="VHT22_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX3136670.1"
                     /translation="MPPAATGATALRDVSLDDKYQLESGRVYLTGVQALVRLLILQRQ
                     RDAAAGLNTAGFVSGYRGSPLGALDQSLWSAGKFLERAHIKFQPGLNEDLAATSIWGS
                     QQVNMYPGAKYDGVFSMWYGKGPGVDRCGDVFKHANYAGTSKHGGVLVLAGDDHAAKS
                     STLPHQSDHQFAAAMMPVLYPSSVQEIIDLGLHGWAMSRYSGLWVGFKCVSDTVESSA
                     SVTIDPDRVKLVMPDDFPFPPDGVHIRWPDGFLETEARMQDFKIYAALHYCRANQLNR
                     IVIDSPKPRLGIITSGKSYLDVRQALDDLGISEADAAEIGIRVFKVAMPWPLEPEGVR
                     HFAEGLDEILVVEEKRQIVEYQLKEQLYNWRTDVRPRVVGKFDEKGEWMRPHGDWLLP
                     AASELTPAMIARVIAQRIARLELHPRHKEKIENRVAFINAKEAALAKPKISLQRIPYF
                     CSGCPHNTSTKVPEGSRSTAGIGCHVMAIWMDRSTSTFTHMGGEGVPWIGQAPFTEEK
                     HIFANLGDGTYYHSGVLAIRASVAAKVNITYKILYNDAVAMTGGQPVDGPISPAIIAR
                     QVAAEGVEKIVVVSDEPDKYPSGYFAAGTAIHHRDDLDKVQRELRDTPGCTVLLYDQT
                     CAAEKRRRRKRGKYPDPAKRVFINELVCEGCGDCGVKSNCVSVAPVETEFGRKRTIDQ
                     SSCNKDFSCVNGFCPSFVTVEGGGLRKPKRADALDVTALPDPAVPATTEPYGILVTGI
                     GGTGVVTIGALLGMAAHLAGKGCSVLDMTGLAQKNGAVVSHVR"
     gene            2559..3236
                     /locus_tag="VHT22_03615"
     CDS             2559..3236
                     /locus_tag="VHT22_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX3136671.1"
                     /translation="MSDLLPAIELETATGPAASVIWLHGLGADGNDFVPIVPELALPP
                     AAAVRFVFPHAPVRPVTINNGMRMRAWYDIAAPDLNNRADITGVRQSQGEIEALITRE
                     KARGVPAARIVLAGFSQGGAIALYAGLRHAERLAGILALSTYLVQADKLPAEAAAANR
                     DVPIFMAHGTLDPVVRFEWGLASRRALEANGNPVEWHQYPIEHSLCLEEVRAIGAWLG
                     KILPPVR"
     gene            complement(3239..>3533)
                     /locus_tag="VHT22_03620"
     CDS             complement(3239..>3533)
                     /locus_tag="VHT22_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3136672.1"
                     /translation="PLHNAVIVRTASGPTDVGRISLTRHWRSIFLRDIELAAGERGHG
                     IGSAILGAVIALAEDSERHVELNVLACNPARHLYARLGFRVVRDDGARLRMRT"
BASE COUNT          588 a         1139 c         1175 g          631 t
ORIGIN      
        1 gcgcacgtgc gatacgacgg caccgttctt ttgcgcgagc ccagtcatgt cgagcacgct
       61 gcaacctttg cccgcgaggt gagcggccat gcccagcaat gcgcctatgg tcacgacgcc
      121 ggtgccgccg ataccggtga ccagaattcc gtaaggctcg gtcgtcgccg gcaccgccgg
      181 atcgggcagc gcggtgacat cgagcgcatc ggcacgcttc ggcttgcgca agccgccgcc
      241 ctcgacggtg acaaagcttg gacaaaagcc gttgacgcag gaaaaatcct tgttgcacga
      301 gctctgatcg atggtgcgct tgcggccgaa ctcggtctcg acgggagcta ccgaaacgca
      361 gttggacttg acgccgcagt cgccgcagcc ttcgcagacc agctcattga tgaatacgcg
      421 cttcgccgga tccgggtact tgccgcgctt gcggcgccgg cgcttttccg ccgcgcaggt
      481 ctggtcgtac agaagcaccg tgcagcccgg cgtgtcgcgg agctcgcgct gtaccttgtc
      541 caggtcgtcg cgatggtgga tcgcggtgcc ggcggcaaaa tagccggatg gatacttgtc
      601 cggctcgtcg ctgacgacga cgatcttttc gacgccttcg gcggcgacct gccgagcgat
      661 gatcgcgggc gagatcgggc cgtcgaccgg ctggccgccg gtcattgcga ccgcatcgtt
      721 atagagaatc ttgtaggtga tgttgacctt ggccgcgacc gacgcgcgga tcgccagcac
      781 gccggaatga taataggtgc cgtcgccgag gttggcgaag atgtgtttct cttcggtaaa
      841 aggcgcctgc ccgatccagg gcacgccctc gccgcccatg tgcgtgaacg tcgacgtcga
      901 gcgatccatc cagatcgcca tcacgtggca gccgatgccc gcggtcgagc ggctgccttc
      961 aggcaccttg gtcgacgtgt tgtgcgggca accggagcag aagtagggga tgcgctgaag
     1021 gctgatcttc ggcttggcca gcgccgcctc cttggcgttg atgaacgcga cgcgattttc
     1081 gatcttctcc ttgtgccgcg ggtgcaactc gagacgcgcg atgcgctgcg cgatcacgcg
     1141 cgcgatcatc gccggcgtca gctcgcttgc cgcgggcaga agccagtcgc cgtgcggccg
     1201 catccactcg cccttttcgt cgaatttgcc gacgacgcgc gggcggacat cggtgcgcca
     1261 gttgtacagc tgctctttca actggtattc gacgatctgc cgtttttcct cgaccaccag
     1321 gatctcgtcg aggccttcgg caaagtggcg cacgccttcg ggctcgagcg gccagggcat
     1381 cgccaccttg aacacgcgga tgccgatttc ggcggcgtcg gcctcgctga tgccgaggtc
     1441 atcgagcgcc tggcgcacgt cgagatagct tttgcccgag gtgatgatcc cgaggcgagg
     1501 cttcgggctg tcgatgacga tgcggttgag ctgattggcc cggcaataat gcagcgcggc
     1561 gtagatcttg aaatcctgca tccgcgcttc ggtctcgagg aaaccgtcgg gccagcgaat
     1621 gtgcacgccg tccggcggaa acgggaagtc gtcgggcatc accagcttga cccgatcggg
     1681 atcgatggtg acggaagccg agctttcgac ggtgtcggac acgcatttga aaccgaccca
     1741 gaggcccgag tagcggctca tcgcccagcc atgcaggccg aggtcgatga tctcctggac
     1801 cgacgacgga tagaggaccg gcatcatcgc ggcggcaaac tggtgatccg actgatgcgg
     1861 caaggtcgac gacttggccg catggtcgtc acccgccagg accagcacac cgccgtgctt
     1921 cgaagtgccg gcgtaattgg cgtgcttgaa cacgtcgccg cagcggtcga cgcccgggcc
     1981 tttgccgtac cacatcgaaa agacgccgtc gtatttggcg cccgggtaca tgttgacctg
     2041 ctggctgccc cagatcgacg tcgcggcaag atcctcgttc aagcccggct ggaacttgat
     2101 gtgcgcgcgc tcgaggaact tgcccgccga ccacagcgac tgatcgaggg cgcccaaggg
     2161 cgaaccgcga tagccggaga cgaagcccgc ggtattgagt ccggccgcgg cgtcgcgctg
     2221 ccgctgcagg atcagcaggc gaaccagcgc ctgaacgccg gtcagataga cgcggcccga
     2281 ctcgagttgg tatttgtcgt cgagagaaac gtcacgcagc gcagtcgcgc cggttgcggc
     2341 agggggcatc ggacaagctc caaaaatttt gtctctggat cgcgcggtca ccggataggg
     2401 gccgccacca cggtcggggc gattgtgtcg ccccgccgtt tcgaggcgcg gattgtacgc
     2461 ccgtttgcgc ccgttagaca ggccggcggc gcatacgttt gccggccgcg acgcccggtc
     2521 cggtagaatg ccggcctttc gccaaggttt tgcgtgagat gtccgatctt ttgcccgcga
     2581 tcgagctcga gaccgcgacc ggccctgcgg ccagcgtgat ctggctgcat gggctcggtg
     2641 ccgacggcaa cgatttcgtt cctatcgttc ccgagctcgc cttgccgccg gccgcggccg
     2701 tgcgctttgt gtttccgcat gcaccggtgc gcccggtgac aatcaacaac ggcatgcgca
     2761 tgcgcgcctg gtacgacatt gccgcccccg acttgaacaa ccgcgccgat atcaccggcg
     2821 tgcgccagtc gcaaggcgag atcgaggcgc tgatcacccg ggaaaaggca cggggcgtgc
     2881 cggcggcgcg gatcgtcctc gccggcttct cgcagggtgg agcgatcgcg ctgtacgccg
     2941 ggctgcgaca cgccgagcgg ctcgccggca tcctggccct atccacctat ttggtgcagg
     3001 ccgacaagct gccggccgaa gcggccgccg ccaaccgcga tgtgcccatt ttcatggcgc
     3061 atggcacgct cgatccggtc gtacgcttcg aatgggggct cgcgtcgcgc cgggcgctcg
     3121 aggcgaacgg caaccccgtg gaatggcatc agtatccgat cgagcactcg ctctgcctcg
     3181 aggaggtgag ggcgatcggc gcctggctag gcaagattct gccgccggtg cgttagcgct
     3241 aggtgcgcat ccggagtcgc gcgccgtcgt ctcgaacgac gcgaaatccc agcctcgcat
     3301 agaggtgacg tgcaggattg caggccagga cgttgagctc gacgtgccgc tcgctgtctt
     3361 ccgccagcgc gatcaccgcc ccgaggatcg cgctgccgat gccgtgcccg cgctcgccgg
     3421 cggcaagctc gatgtcgcga agaaaaatcg agcgccagtg ccgggtcagg ctgatgcggc
     3481 cgacgtcggt gggcccgctt gccgtgcgca cgatcacagc attgtgcagc ggc
//
LOCUS       DATANS010000257         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_434328, whole genome shotgun sequence.
ACCESSION   DATANS010000257 DATANS010000000
VERSION     DATANS010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434328"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /gene="lysS"
                     /locus_tag="VHT22_03625"
     CDS             complement(<1..1213)
                     /gene="lysS"
                     /locus_tag="VHT22_03625"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX3136673.1"
                     /translation="MSEKSDASVPPADENQIIAERRGKLSALRTRGNAYPNDFNRDAL
                     AGELHDAHGQKTHEELDRQAITVSVAGRMLLKRVMGKASFATLQDMSGRIQLYITTDA
                     LGAEAHDAFKHWDLGDIVGARGALFKTKTGELTVKVTELRLLAKALRPLPEKFHGLTD
                     QEQKYRQRYVDLITSPESRRVFIARSAIVQAMREFFVARGYLEVETPMMHPIPGGAAA
                     RPFITHHNALDADLYLRIAPELYLKKLVVGGLEKVFEINRNFRNEGISTRHNPEFTML
                     EFYEAFRDYRYLMDLTEALLREVAQKVCGATTIQYQDASIDLGKPFDRLTMPEAIAKY
                     DPRHAPADLARPEYLKAALAKLGVGVLGSDGLGVLQLKLFEATTEDKLVQPTFIVAHP
                     TDVSPLARASDA"
     gene            complement(1215..>2141)
                     /gene="prfB"
                     /locus_tag="VHT22_03630"
     CDS             complement(1215..>2141)
                     /gene="prfB"
                     /locus_tag="VHT22_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX3136674.1"
                     /translation="KEKKQLEMVVDSLRRIDRTLKDTTELFELARGEEDDATLDVLER
                     DAAGVAATVEDLEFRRMFHNPMDPNSCFIDIQAGSGGTEAQDWAAMLLRMYLRYCERR
                     GYKTEILEESPGEVAGIKSASVKVTGDYAFGYLRTETGVHRLVRKSPFDSNARRHTSF
                     ASVFVYPEVDETIEVEINPADIRIDTFRASGAGGQHINKTDSAVRITHLPTNIVVQCQ
                     NDRSQHRNRAEAMAMLKSRLYELELRNRQAEQQKLEDAKTDIGWGHQIRSYVLDQSRI
                     KDLRTNVEVGNTQAVLDGNLDPFIAASLKQGV"
BASE COUNT          326 a          688 c          684 g          443 t
ORIGIN      
        1 tcgcgtcgct ggcgcgcgcg agcggggaga cgtcggtagg atgcgccacg atgaacgtcg
       61 gctggacgag cttgtcctcg gtcgtcgcct cgaacagctt gagctggagc acgccgagac
      121 cgtcgctgcc cagcaccccg acgccaagct tcgccagtgc tgccttcagg tattccgggc
      181 gcgccagatc ggccggcgca tgccgagggt cgtacttggc gatcgcttcc ggcatcgtca
      241 ggcggtcgaa cggcttgccc aggtcgatcg acgcatcctg gtattggatc gtcgtcgcgc
      301 cgcacacctt ctgcgcgact tcgcgcaaca gcgcctcggt caggtccatc aggtagcgat
      361 agtcgcggaa tgcctcgtag aactcgagca tcgtgaactc ggggttatgc cgggtcgaaa
      421 tcccctcgtt gcggaaattg cggttgatct cgaacacttt ctcgaggccg ccgacgacga
      481 gtttcttcag gtatagctcg ggcgcgattc tcaggtagag atcggcgtcc agcgcgttgt
      541 ggtgggtgat gaacggccgc gccgccgcgc cgccggggat cggatgcatc atcggcgttt
      601 cgacttcgag ataaccgcgc gcgacaaaga attcgcgcat cgcctgcacg atcgccgagc
      661 gggcgatgaa gacgcgccgt gattccggac tcgttattag gtcgacatag cgctgccggt
      721 acttctgctc ctggtcggta aggccgtgga atttctccgg caacggacgc agcgccttgg
      781 ccagcaggcg cagctcggtc accttgaccg tcagctcgcc ggtcttggtc ttgaacagcg
      841 cgccgcgcgc gccgacgata tcgcccagat cccagtgctt gaaagcgtcg tgcgcctcgg
      901 cgcccagggc atcggtcgtg atgtagagct ggatacggcc gctcatgtcc tgcagcgtgg
      961 cgaagctcgc cttgcccatc acccgcttga gcagcatccg ccccgcgact gataccgtga
     1021 tggcctgtcg atcgagctcc tcgtgcgttt tctggccatg cgcgtcatgc agctcgccgg
     1081 cgagcgcgtc gcggttaaaa tcgtttggat aggcgttccc gcgcgtgcgc aatgcactga
     1141 gcttgccgcg tcgctcggca atgatctggt tctcgtccgc cggcggaacc gacgcgtcgc
     1201 tcttctcgct cacctcacac cccctgcttc aggctcgccg cgatgaacgg atcgagattg
     1261 ccgtcgagca ccgcctgcgt gttgccgacc tcgacgttgg tgcgcaggtc cttgatgcgt
     1321 gactggtcga gcacgtaact tcggatctgg tgaccccagc cgatgtcggt cttggcgtcc
     1381 tcgagcttct gctgctcggc ctgacggttg cgcagttcca gctcgtacag gcgcgacttg
     1441 agcatcgcca tcgcctcggc gcggttgcga tgctgtgagc ggtcgttctg gcactgcacg
     1501 acgatgttgg tcggcagatg cgtgattcgc accgccgagt cggtcttgtt gatgtgctgg
     1561 ccgccggcgc ccgacgcgcg gaacgtgtcg atgcgtatgt cggccggatt gatttcgacc
     1621 tcgatcgtct cgtcgacttc cgggtagacg aacacgctgg cgaacgacgt atggcggcgc
     1681 gcgttcgaat cgaatgggct cttgcgcacc agccggtgga ccccggtctc ggtgcggaga
     1741 tagccgaatg cgtagtcgcc ggtcaccttg acgctggcgc tcttgatgcc cgcgacctcg
     1801 cccggcgact cttcgaggat ctcggtcttg tagccgcgac gctcgcagta acgcaggtac
     1861 atgcgcagca gcatcgctgc ccaatcctgc gcctcggtgc ctccgctgcc tgcctggatg
     1921 tcgataaaac aggaattcgg gtccatcgga ttgtggaaca tccggcggaa ctcgagatcc
     1981 tcgaccgttg cggcgacgcc agcggcatcg cgttccagga cgtcgagcgt cgcgtcgtcc
     2041 tcctcgcccc gggccagctc gaacagctcg gtcgtgtcct tgagcgtccg gtcgatccgg
     2101 cgaaggctgt cgacgaccat ctccagctgt tttttttcct t
//
LOCUS       DATANS010000258         4833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_182316, whole genome shotgun sequence.
ACCESSION   DATANS010000258 DATANS010000000
VERSION     DATANS010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182316"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VHT22_03635"
     CDS             complement(<1..852)
                     /locus_tag="VHT22_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEX3136675.1"
                     /translation="MSDDSNDRSTKPTLLERLSAFLSREPDDREELLRLLHGAFEHKL
                     LDADALSIIEGALQVSEMTVRDIMIPRAQMDVVSIEDEPPQFIPFVLEKRHSRFPVIG
                     ENKDDVVGILLAKELLNYYANPESFELHDTLRPVVFVPESKRLNVLLRDFRANRNHIA
                     IVVDEYGGVSGLVTIEDVLEQIVGDIEDEYDFDEAEDNIIAEANGRYRVKAQTEIAAF
                     NEKFGSEFSDEEFDTIGGLVLRAFGRLPKRGETTTIDKFRFRVVRADSRRIYTLQVEK
                     LSPPPEEE"
     gene            complement(849..1316)
                     /gene="ybeY"
                     /locus_tag="VHT22_03640"
     CDS             complement(849..1316)
                     /gene="ybeY"
                     /locus_tag="VHT22_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX3136676.1"
                     /translation="MTAPRLTLTVQRASDDADLPARGLLRRCVAAALERDADITLRFV
                     DRDEARRLNRRYRRQDHPTNVLSFVYDDERDVLRGDIVLCTPVLRREAQRQGKALAAH
                     CAHLVVHGTLHLQGYDHVDDADAARMEAHEATILARLGYADPYAAELPSRSAR"
     gene            complement(1313..2443)
                     /locus_tag="VHT22_03645"
     CDS             complement(1313..2443)
                     /locus_tag="VHT22_03645"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEX3136677.1"
                     /translation="MRADEQDALRRRCAIVVSGHGDDAPGAELARVAEWCSRNGWHAD
                     RYGEGALIGAFEARVAALLGKPAAAFMPSGTMAQQIALRIACDRANNSVIAMHPTSHL
                     ELHEEHAYARLHSLRAILLGERKRPTSARDLDELAETPAALLVELPAREIGGRLPDWP
                     ELVALMTLARERGIWRHMDGARLWEAREHYAPASYADICAQFDSVYVSFYKGIGALAG
                     AMLLGSEDFIAEARVWRRRHGGTLVQLQPFVASAARRFDGQLAKMAAYRARAAAFAES
                     LAGIDGLSVEPAPPQCNLFHLHFHAPAEALTAARDRIAGEDSAWLAPRFSADRGPGLA
                     SAEIYVGDSLLAVPRGEDRRLLAPLYARMVDLARAGGTAAAQ"
     gene            complement(2498..3511)
                     /locus_tag="VHT22_03650"
     CDS             complement(2498..3511)
                     /locus_tag="VHT22_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX3136678.1"
                     /translation="MKTQTLDVELSPVDNRALANLCGALDANLRQIESALDVSIARRG
                     AAFTVRGQPAQTQQAATALERFYAEAHAPLSIDDIQLGLVELRSVAAREATPHTADAS
                     AEALTLRTRRADLHGRTANQILYLKHIQEHDITFGTGPAGTGKTYLAVACAVDALERD
                     LVKRIVLVRPAVEAGERLGFLPGDLAQKVDPYLRPLYDALYDLMGFDKTARLFERNTI
                     EIAPLAYMRGRTLNHSFIILDEAQNTTPEQMKMFLTRIGFGTKAVITGDVTQIDLARG
                     QKSGLIEARRVLADVRGIAFTEFTAADVVRHPLVQKIISAYEKHTRSERIDAPGASSG
                     GER"
     gene            complement(3517..>4833)
                     /locus_tag="VHT22_03655"
     CDS             complement(3517..>4833)
                     /locus_tag="VHT22_03655"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX3136679.1"
                     /translation="DRQDFLIAEGLLAPYEKGKAPNPQLMQMVLARLRQLAAHEVGHT
                     LGLQHNFRASTIYTQKQLEDPVFTQANGIGGSVMDYNAINLALQNEKQGDYVMPTIGP
                     YDYWAIEYAYKPIEPDKEKSELAKIAARSDEPQLAFANDIDAGFGGVAEGMDPQVNRR
                     DLGSDPLEFATRRLKLSRELWARLQDRKLAPGQSYEVLRRNFVAGLTQLGNAGAVAAK
                     YVGGVVYVRDHAGSGREPFTPVAPDKQRAALKLIADGLLSVDSFRLRPEFVRRLTVDQ
                     FDRGREDAGISAISPDISLSDRMLRVQRALLDQLLSDTVATRIEESAYRQTKSSEAFR
                     LSELYDTLQSAVWSELKTGRDITPFRRNLQREYLRRVATTILHPAPSDHADGRALQRA
                     NAKELLAQLKAAQSRASFSKEARAHLAECENTLYEALKAPMLRPDA"
BASE COUNT          761 a         1592 c         1631 g          849 t
ORIGIN      
        1 ttcctcttcg ggcggcggcg agagcttttc cacttgcagc gtgtagatcc ggcggctgtc
       61 ggcgcggacg acgcgaaagc ggaatttgtc gatcgtcgtc gtctcgcccc gctttggcag
      121 gcggccgaac gccctcaaca ccagtccgcc gatggtgtcg aactcctcgt cgctgaactc
      181 gctgccgaac ttctcgttga aggcggcgat ctcggtctgc gccttgaccc gatagcggcc
      241 gttcgcctcg gcaatgatgt tgtcctcggc ctcgtcgaag tcgtactcgt cctcgatatc
      301 gccgacgatc tgctcgagca cgtcctcgat cgtcaccaga cccgagacgc cgccgtattc
      361 gtcgacgacg atcgcgatgt ggttgcgatt ggcacgaaag tcgcgcagca gcacgttgag
      421 ccgcttcgat tcgggcacga aaacgaccgg ccgcagcgtg tcgtgcagct cgaagctttc
      481 cggattcgcg tagtagttga gcagttcctt cgccagcagg atgccgacga catcgtcctt
      541 gttctcgccg atcaccggaa agcgcgagtg ccgcttttcg agcacgaacg gtatgaactg
      601 gggcggctcg tcctcgatcg aaacgacgtc catctgggcg cgcgggatca ttatgtcgcg
      661 cacggtcatc tcggacacct gcagcgcgcc ctcgatgatc gacagcgcgt cggcatcgag
      721 cagcttgtgc tcgaatgcgc cgtgcagcag cctgagcagc tcttccctgt cgtcgggttc
      781 gcgcgagagg aaagccgaca gacgctcgag cagcgtcggc ttcgtcgacc ggtcgttgga
      841 gtcgtcgctc atcgggcgct gcgggacggc aattcggcgg cgtaagggtc ggcgtagccc
      901 agccgcgcta ggatggtggc ctcgtgtgcc tccattcgtg ccgcatcggc gtcgtcgacg
      961 tggtcgtagc cttgcagatg cagcgtgcca tggacgacca ggtgcgcgca gtgcgcagcc
     1021 agtgccttgc cctgccgttg cgcctcgcgc cttaacaccg gagtgcagag cacgatatcg
     1081 ccgcgcaata cgtcacgctc gtcatcgtac acgaacgaga ggacgttcgt cgggtggtcc
     1141 tggcgccggt aacggcggtt cagccgtcgc gcttcgtcgc ggtcgacgaa acgcagcgtg
     1201 atgtcggcgt cgcgctcgag tgcggccgcg acgcagcgcc gaagcaatcc ccgtgccggc
     1261 agatcggcat cgtcggaagc gcgctgcacg gtaagggtca ggcgcggcgc ggtcattgcg
     1321 ccgccgccgt cccgcctgcg cgcgccaggt ccaccatcct ggcgtacagc ggtgcgagca
     1381 gacggcggtc ctcgccgcgc ggaaccgcca gcaagctgtc gccgacgtag atttcggcgc
     1441 tggcaagacc ggggccgcgg tccgcggaga aacgcggcgc gagccacgcg ctgtcctcac
     1501 ctgcgatacg atcgcgcgcc gcggtcagcg cttccgctgg cgcatggaaa tgcagatgga
     1561 acagattgca ttgcggcggc gccggctcga ccgacaagcc gtcgatgccg gcgagcgatt
     1621 cggcaaaagc ggccgcccgc gcgcggtacg ccgccatctt ggcgagttgc ccatcgaagc
     1681 gcctcgcagc tgacgcgacg aacggctgca gctggaccag ggtgccccca tgccggcgcc
     1741 gccagacccg cgcctcggcg atgaagtctt ccgagcccag cagcatggcg cccgccagcg
     1801 cgccgatgcc tttgtagaac gagacgtaga ccgaatcgaa ctgcgcgcag atgtcggcgt
     1861 aggaggcggg tgcgtagtgt tcgcgcgctt cccagagcct cgcgccgtcc atgtggcgcc
     1921 agatgccgcg ctcgcgggcg agcgtcatca gggcgaccag ctccggccag tccggcagcc
     1981 ggccgccgat ctcgcgcgcc gggagttcga ccagcagcgc ggccggagtt tcggccagct
     2041 cgtcgagatc gcgcgccgaa gtcggccgct tgcgctcacc caggagaatc gcccgcaacg
     2101 aatgcaggcg cgcgtaggca tgctcctcgt gcagctcgag atgcgaggtc ggatgcatcg
     2161 cgatgaccga gttatttgcg cggtcgcagg cgatgcgcag cgcgatctgc tgagccatcg
     2221 tgccgctggg catgaacgcg gcggcgggct tgcccaggag cgcggccacc cgggcctcga
     2281 aggcgccgat cagcgcaccc tcgccgtagc gatcggcatg ccatccgttc cggctgcacc
     2341 attcggcgac acgcgccagc tcggcgccgg gcgcgtcatc gccgtggccc gaaacgacga
     2401 tcgcgcagcg ccggcgcagc gcatcctgct cgtcggcgcg caacggcagc gatgcaagcg
     2461 ggcgagtcag ggtttcgccg ggaaaacgcg cggcacgcta gcgctcgccg ccggagctcg
     2521 cgcccggggc atcgatccgt tcgctgcgcg tgtgcttttc ataggcgctg attattttct
     2581 ggaccagcgg atggcgcacg acgtcggcgg cggtgaattc ggtgaaggcg atcccgcgaa
     2641 cgtcggccag cacgcggcgc gcctcgatca gcccgctttt ctggccgcgg gcaagatcga
     2701 tttgcgtgac gtcgccggtg atcacagcct tggtcccgaa cccgatgcgc gtcagaaaca
     2761 tcttcatctg ctccggtgtc gtgttctgcg cttcgtcgag aatgatgaac gagtgattca
     2821 gcgtccgacc tcgcatgtag gcgagcggcg cgatctcgat cgtgttgcgc tcgaacagcc
     2881 gcgccgtctt gtcgaagccc atcaggtcgt aaagcgcgtc atagagtggc cgcagatacg
     2941 ggtcgacttt ctgcgccaga tcgccgggga ggaagcccag gcgctcgcct gcctcgaccg
     3001 caggccgcac cagcacgatg cgcttgacca gatcgcgctc gagcgcgtcg accgcgcacg
     3061 caaccgccag gtaggtcttt ccggtgccgg ccggcccggt gccgaacgtg atgtcgtgct
     3121 cctggatgtg cttcaggtac aggatctggt tggccgtgcg cccgtgcaga tcggcgcgcc
     3181 gcgtgcgcag cgtaagcgcc tcggccgaag cgtctgctgt gtgcggcgtg gcttcgcgcg
     3241 ccgcaacgct gcgcaattcg accagaccga gctggatatc gtcgatggag agcggcgcgt
     3301 gcgcctcggc gtagaagcgc tcgagcgccg tagccgcctg ctgcgtctga gccggctgtc
     3361 cgcgtacggt gaacgcggcg ccgcggcgcg cgatcgagac atccagcgcc gactcgatct
     3421 ggcgcaggtt ggcgtcgagc gcgccgcaaa gattggcgag cgcgcggtta tcgactggcg
     3481 aaagctcgac gtcgagagtc tgcgtcttca aggacactat gcgtcgggcc gcagcatcgg
     3541 cgccttcagg gcttcgtaaa gcgtgttctc gcactcggca agatgcgcgc gcgcctcctt
     3601 cgagaacgag gctcgggatt gggcagcctt gagctgcgcc agcagttctt tcgcgttggc
     3661 gcgctgcagc gcccggccat cggcatgatc ggacggcgcc ggatgcagga tcgtcgtcgc
     3721 cacccgtcgc agatattcgc gttgcaggtt gcgccggaac ggcgtgatat cgcggccggt
     3781 tttcagctcg ctccagacgg cgctttgcag tgtgtcgtag agctcggaca ggcggaacgc
     3841 ctcgctgctc ttcgtctgac ggtaagcgct ttcctcgatg cgcgtcgcca ccgtatcgga
     3901 cagaagctga tcgagcagcg cgcgctgcac gcgcaacatc cggtcggaaa gcgaaatgtc
     3961 cggcgagatc gcgctgatcc ccgcgtcctc gcgcccgcgg tcgaactggt cgacggtgag
     4021 cctgcgcacg aattcagggc gcaggcggaa gctgtcgacc gacagcaacc cgtcggcgat
     4081 cagcttcagc gcggcgcgct gcttgtcggg cgccaccgga gtgaacggct cgcgaccgct
     4141 gccggcatgg tcgcgcacgt agacgacgcc gccgacatat ttggccgcga ctgcgccggc
     4201 gttgcccagc tgcgtcaggc cggcgacgaa attgcgccgc aacacttcgt agctctgccc
     4261 cggcgcaagc ttgcggtcct gaagccgggc ccagagctcg cgggagagct tgagccggcg
     4321 ggtcgcgaac tcgagcggat cgctgcccag gtcgcgccgg ttcacctgcg gatccatgcc
     4381 ttcggccacg ccgccgaaac cggcgtcgat gtcattggcg aaggcgagct gcggctcgtc
     4441 gctgcgcgcg gcgattttgg cgagctcaga cttctccttg tcaggctcga ttggcttgta
     4501 cgcatactcg atcgcccagt agtcgtaagg gccgatcgtc ggcatcacat agtcgccctg
     4561 cttctcgttc tggagtgcca ggttgatcgc gttgtaatcc atcaccgatc cgccgatgcc
     4621 gttcgcttgc gtgaacaccg ggtcttcgag ctgcttttgc gtgtagatcg tcgaagcgcg
     4681 gaagttgtgc tgcagcccca gcgtgtggcc cacttcatgg gccgccagtt gcctgagccg
     4741 cgcgagcacc atctgcatga gctgcgggtt cggggccttg cctttttcat aaggagcgag
     4801 cagaccttcg gcaataagga aatcctggcg gtc
//
LOCUS       DATANS010000259         2128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_12502, whole genome shotgun sequence.
ACCESSION   DATANS010000259 DATANS010000000
VERSION     DATANS010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12502"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VHT22_03660"
     CDS             complement(<1..458)
                     /locus_tag="VHT22_03660"
                     /inference="COORDINATES: protein motif:HMM:NF015523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HEX3136680.1"
                     /translation="MTTPPAPPPTPPSVVPAPPEPAKAMLRIATYAAIPGWRDDSAPA
                     AWPAFVAGCAPLLQSPAASIWRESCEAAPAVDVRNDTEVRAFFEAHFTPYQLTGADGR
                     DTGVVTGYYEPLLQGSRQPGGRYRFPLYATPDDLLVVELGELFPELKDKRV"
     gene            517..654
                     /locus_tag="VHT22_03665"
     CDS             517..654
                     /locus_tag="VHT22_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136681.1"
                     /translation="MEWLLLEALVALALAVFIVWFTMGGKRKNPPRDPKDPSDGDDAG
                     G"
     gene            complement(728..1111)
                     /gene="apaG"
                     /locus_tag="VHT22_03670"
     CDS             complement(728..1111)
                     /gene="apaG"
                     /locus_tag="VHT22_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEX3136682.1"
                     /translation="MGESSKYEITVEPRATYVEDQSDPSKNQYVFAYTISVTNTGTVP
                     AQLISRHWVITDANNKVMEVKGLGVIGQQPLLKPGESFEYTSGTHLETAVGTMHGTYQ
                     MVAEDGQHFDAPIPSFTLSVPRILH"
     gene            1154..1867
                     /gene="rpe"
                     /locus_tag="VHT22_03675"
     CDS             1154..1867
                     /gene="rpe"
                     /locus_tag="VHT22_03675"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX3136683.1"
                     /translation="MREVQIAPSILSADFARLGEEVRAVIAAGADLIHFDVMDNHYVP
                     NLTVGPLVCAALKPHAGVPIDVHLMVRPVDALVPAFADAGAAIISFHPEASDHVDRTI
                     GLIHERGCRAGLVLNPATPLSCLDHVLDKLDLVLIMSVNPGFAGQSFIPEALHKLSLA
                     RERIDAVAARSGRSIRLEVDGGVKIDNIGAIARAGADTFVAGSAIFGSGDYSKTIAAM
                     RAAANAAGRAAALRPGVSA"
     gene            1864..>2128
                     /locus_tag="VHT22_03680"
     CDS             1864..>2128
                     /locus_tag="VHT22_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX3136684.1"
                     /translation="MTIAPSPRIRVRAIAFDLDGTLLDTIHDLATAINLMLARLNQPA
                     LSKDTIRAMVGKGMANLVRRALAAAQGSPPTPSDEARALALYET"
BASE COUNT          372 a          666 c          713 g          377 t
ORIGIN      
        1 actcgtttgt ccttgagctc ggggaagagc tcgcccaact cgacgacgag caaatcgtct
       61 ggcgttgcgt agagcggaaa acgatagcgt ccgccgggct gccggctgcc ctgcagcaag
      121 ggctcgtaat agccggtcac gacacccgtg tcgcgcccgt cggctccggt aagctggtat
      181 ggggtaaaat gcgcttcgaa aaacgcgcgc acctcggtgt cgttgcggac atcgacggct
      241 ggcgcggctt cgcagctttc gcgccagatc gacgcagcag gcgactgaag gagcggcgcg
      301 cagcccgcga cgaatgccgg ccaggcggcc ggcgcgctgt cgtcgcgcca accgggaatt
      361 gcggcgtaag tggcgatgcg cagcatcgcc ttcgccggct cgggcggtgc gggcacgacc
      421 gatggaggcg tgggcggcgg cgcggggggc gttgtcacgc aaccggcaat ggacgcgatc
      481 agcagcgcgg ccagcagctg aaggaaaggc gagccgatgg agtggctgtt gctcgaagcc
      541 ttggtggcgc tggcgctcgc ggtgtttatc gtctggttta ccatgggcgg gaagcgaaag
      601 aatcccccgc gtgacccgaa ggatccgagc gacggcgacg acgccggagg ctgaaggtgc
      661 ggcgcaactt gggcggcgcg cgtgagaaca gatcggcgcg ttcggacgaa tcgaaagaaa
      721 acggacacta atgcagaatg cgcggcaccg aaagcgtaaa cgaggggatc ggcgcgtcga
      781 aatgctggcc gtcttctgcc accatctgat aggtgccatg catcgtgccc accgccgttt
      841 ccagatgggt gccgctggtg tactcgaagc tctcacccgg cttgagcagc ggttgctgac
      901 cgataacgcc gaggcccttg acttccatta ccttgttgtt ggcatcggtg atcacccagt
      961 gccggctgat cagctgcgcg gggaccgtac cggtgttggt caccgagatc gtgtaagcaa
     1021 atacatattg attcttcgac ggatccgact ggtcctcgac gtacgtcgct cgcggctcga
     1081 ccgtgatctc gtatttgctg gactcgccca tcggcgtgct ctttaagtct tgtccctgag
     1141 cgattataac gccatgcgcg aggtccagat cgcaccgtcg attctctccg ccgatttcgc
     1201 ccggctcggc gaggaagtgc gcgcggtgat cgccgctggc gccgacctga tccacttcga
     1261 cgtgatggac aatcattacg ttccgaacct gacagtcgga ccgttggtgt gcgcagcgct
     1321 gaagcctcac gccggcgtcc cgatcgacgt gcatctgatg gtgcgaccgg tcgacgcgct
     1381 ggtccctgca ttcgccgacg cgggggccgc gatcatcagc tttcatcccg aagcgagcga
     1441 ccatgtcgat cgcacgatcg gcttgataca cgagcggggc tgcagagcgg gcctcgtgct
     1501 caatcccgcg accccgcttt cctgtctcga ccatgtgctc gacaagctcg acctggtgct
     1561 gatcatgtcg gtcaatccgg gcttcgccgg ccagtcattc attcccgagg ccttgcacaa
     1621 gctctcgctg gcgcgcgagc gcatcgacgc cgtcgcggcg cgcagcggaa ggtcgatccg
     1681 gctcgaagtc gacggcggcg tcaagatcga caacatcggc gcgatcgccc gggcgggcgc
     1741 ggacaccttt gttgccggat cggcgatttt cggcagcggc gattactcga agacaatcgc
     1801 cgcgatgcgc gccgcggcga atgcggcggg ccgtgccgcg gcgcttcgtc cgggagtatc
     1861 cgcatgacga tcgcaccatc cccgcgtatt cgcgtgcgcg cgatcgcctt cgatctcgat
     1921 gggacgctgc tcgacaccat ccacgacctg gccacggcga tcaatctgat gctggcgcgc
     1981 ttgaaccagc cggcgctgtc caaggacacg atacgcgcga tggtgggcaa gggaatggcg
     2041 aatctggtcc gacgcgctct cgccgcggcg caaggatcgc cgccgacgcc gagcgacgag
     2101 gcgcgagcgc ttgcgctgta cgaaacgg
//
LOCUS       DATANS010000260         1674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_644154, whole genome shotgun sequence.
ACCESSION   DATANS010000260 DATANS010000000
VERSION     DATANS010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644154"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="VHT22_03685"
     CDS             complement(<1..131)
                     /locus_tag="VHT22_03685"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX3136685.1"
                     /translation="MHCFALWVEFDLRPGHLAAFLEAARLDAEGSVGNEPGCRRFDIL
                     "
     gene            complement(159..1151)
                     /locus_tag="VHT22_03690"
     CDS             complement(159..1151)
                     /locus_tag="VHT22_03690"
                     /inference="COORDINATES: protein motif:HMM:NF020481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEX3136686.1"
                     /translation="MHKLQAMVAAQPRGTPRAPPLWNELVDPDVRALAALIASPLLIA
                     DHAAPIATLTDAWRIESLRLRHGWLLDLDAAPQRLREHLAASNARQLGRYAEALWRFW
                     FAHLPGARLHAAGLPVKDALAVRGEFDFIVSLPGLPEVQHLEMAYKFYLYCPPGDDFS
                     RLFGPNAADRLDRKWQHMLDVQLPLSQTELGRAALPQDVGQVTPRACLQGWLFSPLAT
                     GSTAPIAGVSPAHWRGWWCRSDDWRSHGAPWTELAGAWSLLPRRAWIAPAAVDDPQAM
                     LSPDACRTRIDAHFAASSQAQLVAGLQRASDGIWRETVRIFVVAPHWPRGSAAT"
     gene            1336..>1674
                     /locus_tag="VHT22_03695"
     CDS             1336..>1674
                     /locus_tag="VHT22_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136687.1"
                     /translation="MATKSSSSTARRTRAAGSKARKPRAKTAAKQPSPFDAPRAGAFG
                     DLSKMGKLMTPEQAFELYRANAKIALDVINAAIESTAKLRRLQFEGEEQARSMQKKAA
                     RHAAEAGDPQA"
BASE COUNT          300 a          558 c          581 g          235 t
ORIGIN      
        1 aggatgtcga accgccggca cccgggctcg ttgccgaccg acccttcggc gtcgagtcgg
       61 gcagcctcga gaaacgcggc gagatgcccg ggacgcaggt cgaattccac ccagagtgca
      121 aagcagtgca taggcttttc cgatcgatgg tgctgccgct acgtcgcggc gctgccccgt
      181 ggccagtgcg gcgccacgac aaatatgcgg accgtctcgc gccagatgcc atccgatgcg
      241 cgctgcaggc cggcgactag ttgagcttgt gacgaggctg cgaaatgtgc atcgatcctt
      301 gtccggcaag cgtccggcga gagcatcgcc tgcggatcat cgaccgccgc cggcgcgatc
      361 cacgcgcgcc gcggcagcaa gctccatgcc ccggcgagct cggtccaggg cgcgccgtgg
      421 ctgcgccagt cgtcgcttcg gcaccaccac ccgcgccagt gcgccggcga cacgccggca
      481 atcggcgcgg tcgaccccgt cgccagcgga gagaacaacc agccctgaag acaggcccgt
      541 ggggttacct ggccgacgtc ctgcggcagc gccgcgcggc cgagctcggt ctgcgacagc
      601 ggcaattgca cgtcgagcat gtgctgccac ttgcggtcga gccgatcggc ggcattgggt
      661 ccgaacaggc gcgaaaagtc gtcccccggc gggcagtaga gatagaactt gtacgccatc
      721 tccaagtgct gcacttcggg gagacccggc agcgatacga tgaaatcgaa ttcgccgcga
      781 accgcgagcg cgtccttgac cggcaagccg gcggcgtgca gacgcgcccc gggcagatgg
      841 gcgaaccaga agcgccacag cgcctcggcg tagcggccga gctggcgcgc gttggaagcc
      901 gccaggtgtt cgcgcaggcg ctgcggcgcc gcgtccagat cgagcagcca gccgtggcgc
      961 aagcgcagcg attcgatgcg ccaggcatcg gtcaaagtgg cgatcggcgc cgcatggtcg
     1021 gcgatcagca acggcgatgc gatcagcgcg gccagggctc gcacgtcggg gtcgacgagc
     1081 tcgttccaca acggcggcgc gcgcggcgtc ccgcgcggct gggcggcgac catcgcttgc
     1141 agcttgtgca tcggaaccat ggggggaatg gcgattctaa cctgcgctgg cgcgctgccg
     1201 ggagcgtgct tcgctttccc gttgactgga gcggcgccaa ggcgtgcaat aagcgcacat
     1261 caccaggcac ccagatgtct gcggcgcaag ccgatcttgg gtcgtttttc cattcagcct
     1321 cgaggagtcc ctaccatggc caccaaatcg tcgtcgagta cggcacgccg caccagggcc
     1381 gccggaagca aagcgcgcaa gccccgcgcg aagacggcgg ccaagcagcc gtcgccgttc
     1441 gatgcgccgc gtgccggtgc attcggcgac ctctcgaaaa tgggcaagct gatgacgccg
     1501 gagcaggcgt tcgagctcta tcgcgccaac gcgaaaattg cgctcgatgt gatcaatgca
     1561 gcgatcgaga gcacggccaa gctgcgccgg ctgcagttcg agggagagga gcaggcgcgt
     1621 tcgatgcaga aaaaagcggc gcgccatgcc gccgaggctg gcgatccgca ggcg
//
LOCUS       DATANS010000261         4583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_727912, whole genome shotgun sequence.
ACCESSION   DATANS010000261 DATANS010000000
VERSION     DATANS010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727912"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /gene="dut"
                     /locus_tag="VHT22_03700"
     CDS             complement(<1..444)
                     /gene="dut"
                     /locus_tag="VHT22_03700"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX3136688.1"
                     /translation="MIATVELRVLDERIRPFLPAYATPGSAGMDLRACIDAPIELQPG
                     RTELVPTGVAIHIGDPGLAALILPRSGLGHKHGIVLGNLVGLIDSDYQGQLMVSCWNR
                     GSEAFELKPMDRLAQLVLVPVVQARFEEKKSFDSSSRGEGGFGSTG"
     gene            complement(445..1644)
                     /gene="coaBC"
                     /locus_tag="VHT22_03705"
     CDS             complement(445..1644)
                     /gene="coaBC"
                     /locus_tag="VHT22_03705"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX3136689.1"
                     /translation="MTSPAAARTLSRVVLGLTGGIAAYKAAELTRLLVKEGIAVDVVM
                     TNAAAQFIAPMTLQALSGNPVLSGLWESGAANAMGHIELSRHADAIVVAPASADFIAK
                     LANGHADDLLSTLCLARDCPLLIAPAMNRQMWSNAATQRNVAQLRADGVEILGPGSGD
                     QACGEIGDGRMLEPEEIMAALLASAQPKLLAGKRVLLTAGPTVEAIDPVRAITNHSSG
                     KMGFSLAQACAEAGANVTLVSGPTSLGTPTGVTRIDVKSAAEMAAAVDSAVPAAQIFI
                     AVAAVADYTPAEVRASKLKKNGDRMTLALKPTADILAAVAARPKPPFCVGFAAETDDI
                     VKNAEAKRRSKKVPLLIANRAQDAMGSEENEVTLVDERGAHPLPRMAKLALARRLVAE
                     IASRLPR"
     gene            1730..2404
                     /gene="radC"
                     /locus_tag="VHT22_03710"
     CDS             1730..2404
                     /gene="radC"
                     /locus_tag="VHT22_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX3136690.1"
                     /translation="MRIADWPADERPRERLLKLGAAALSEAELLAIFLRTGMAGSSAV
                     ELGRELLARFGGLNRLFGATLDEFAAVRGFGPAKYVQLQAVVEIARRALSEEIGERDA
                     MSSPRAVRDYLSLSLGSRPHEVFVALFLDSQNRLLGSEELFRGTLTQTSVYPREVVKT
                     ALRYNAAAVILAHNHPSGVAEPSRADELLTQTLKQALALVEIRTLDHFIVAGTRTVSF
                     AERGLL"
     gene            2490..2726
                     /gene="rpmB"
                     /locus_tag="VHT22_03715"
     CDS             2490..2726
                     /gene="rpmB"
                     /locus_tag="VHT22_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX3136691.1"
                     /translation="MARVCQVTGKAPMVGHHVSHANNKTKRRFLPNLQRRRFWVETEN
                     RWVSLRLTNAALRTIDKNGIDVVLADMRARGEHV"
     gene            2757..2912
                     /gene="rpmG"
                     /locus_tag="VHT22_03720"
     CDS             2757..2912
                     /gene="rpmG"
                     /locus_tag="VHT22_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX3136692.1"
                     /translation="MREKIKLESSAGTGHFYTTSKNKKLMPEKMEIKKFDPVARKHVM
                     YKETKLK"
     gene            complement(3001..4209)
                     /locus_tag="VHT22_03725"
     CDS             complement(3001..4209)
                     /locus_tag="VHT22_03725"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX3136693.1"
                     /translation="MSFDPLSLLAHGLVPLPWWGYVVVGLVFTHITIAAVTIYLHRSQ
                     AHRGLDLHPLVSHFFRFWLWLTTGMVTKEWVAIHRKHHAKVETAEDPHSPQTRGINKV
                     FWEGAELYRDEAKNGETLDKYGLGTPDDWIERNVYTRHSWEGVGLMLVVNLALFGPLG
                     ATIWAVQMAWIPVTAAGIINGIGHYWGYRNFSCEDASRNIVPWGILIGGEELHNNHHA
                     YGTSAKLSSRWYEFDLGWAYIRTLEFFGLATVRKVAPRLKFDRVKLNADLETLQAIIT
                     HRYAVLTSYASSLKLAYRQEVASLRERARRGELTPDMPSFRRLRRWFQSDATALPETD
                     RAHLETTLEGSSALKTIYSMRQELAGLWERSNDSREQLLARLQDWCHRAEASNIAALQ
                     KFSRTLRSYA"
     gene            complement(4338..>4583)
                     /locus_tag="VHT22_03730"
     CDS             complement(4338..>4583)
                     /locus_tag="VHT22_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HEX3136694.1"
                     /translation="VLQGALAPIVNVLQFGDNGIALELVVWINDPEMGQGNLRSALNI
                     GIWKAFRDNGIRIPYPQREVRIIGQAPGDNAETSPPL"
BASE COUNT          803 a         1455 c         1516 g          809 t
ORIGIN      
        1 ccctgtactc ccgaatccgc cttcgccgcg gctcgaggaa tcgaacgatt tcttttcctc
       61 gaatcgggcc tgaacaaccg ggacaagcac gagttgcgcc agccggtcca tcggtttgag
      121 ctcaaaagcc tcgctgccgc ggttccagca gctgaccatc agctgtccct ggtaatcgga
      181 gtcgatcagg ccgaccaggt tgcccagcac gatgccgtgc ttgtggccca ggccggagcg
      241 gggcaggatc agcgccgcca ggccgggatc gccgatgtgg atcgccacgc cggtggggac
      301 gagctcggtc cggcccggct ggagctcgat cggggcgtcg atacaggcgc gcagatccat
      361 tcccgccgaa cccggcgttg cgtacgcagg caggaacggc cggatccgct cgtcgaggac
      421 cctgagctcg acggtggcga tcatctaccg cggcagccgc gatgcgatct cggcgaccag
      481 ccgacgcgcg agcgcgagct tggccatccg cggcaacggg tgcgcgccgc gctcgtcgac
      541 cagcgtgact tcgttctcct cgcttcccat cgcgtcctgc gcccgattgg cgatcagcag
      601 gggcactttc ttgctgcggc gcttggcctc ggcgttcttc acgatgtcgt cggtctcggc
      661 ggcaaagccg acgcaaaacg gcggtttcgg acgcgcggcg acggcagcga gaatgtcggc
      721 cgtgggtttg agcgcaagcg tcatccggtc gccatttttc ttcagtttgc ttgcccggac
      781 ttcggccggc gtatagtcgg cgacggcggc gaccgcgata aagatctgcg cggccgggac
      841 cgcggagtcg accgcggccg ccatctcggc cgcgcttttc acgtcgatgc gcgtcactcc
      901 ggtcggcgtg cccagcgacg tcggaccgga taccagcgtg acattcgcgc cggcttcggc
      961 gcacgcctgc gccagcgaga accccatctt gcccgagctg tgattggtga tcgcgcgcac
     1021 cggatcgatc gcttcgaccg tcgggcccgc cgtcagcagc acgcgcttgc cggcgagcag
     1081 ctttggctgc gccgacgcga gcagcgccgc catgatttct tcgggctcga gcatgcggcc
     1141 gtcgccgatt tcaccgcagg cctgatcgcc gcttcccgga ccgaggatct cgacgccgtc
     1201 ggcgcggagc tgcgcgacgt tgcgctgtgt ggccgcgttg ctccacatct gccgattcat
     1261 cgccggcgcg atcagcagcg gacagtcgcg tgccaggcaa agcgtcgaca agagatcatc
     1321 ggcatggccg ttcgccagct tggcgataaa atccgccgac gccggcgcga cgacgatcgc
     1381 atcggcatgg cgcgagagct cgatgtgtcc catcgcgttg gccgcgcccg actcccacag
     1441 gcccgacagc accgggttcc ccgacagcgc ctgcaaagtc atcggtgcga tgaactgcgc
     1501 ggcggcattc gtcatcacga cgtcaacggc gatcccttcc ttgaccagta gccgcgtcag
     1561 ctccgcggcc ttgtaggcgg ctatgccgcc ggtcaggccg agcacgacac gcgaaagcgt
     1621 tcgcgcggca gcgggggagg tcatcgcaga ggaatcatcc atgaggacga acgggggtgt
     1681 ggcgtggcgc agtttaatat gcgctggcat cacggtacag gggctggata tgcgcattgc
     1741 cgattggccg gcggacgagc gtccgcgcga gcgcctgctg aagctcggcg ccgcggcctt
     1801 gtccgaggcc gagctgctcg cgattttcct gcgcaccggc atggccggca gcagcgcagt
     1861 cgagctcggt cgcgagctgc tcgcgcgctt cggcggcttg aatcgcctgt tcggcgccac
     1921 gctcgatgaa ttcgcggcgg tgcgcggctt cggcccggcg aaatacgtgc agctgcaggc
     1981 cgtggtcgaa atcgcgcgcc gcgcactgtc cgaagagatc ggcgagcgcg atgcgatgag
     2041 ctcgccgcgc gcggtgcgcg actacctgtc gctgtcgctg gggagccggc cacacgaagt
     2101 gttcgtcgcg ctgtttctgg attcgcaaaa ccgcctgctg ggatcggaag agctcttccg
     2161 cggcacgctg acgcagacca gcgtgtaccc gcgcgaagtc gtcaagacgg cgcttcgcta
     2221 caatgcagcg gccgtgattc tggcgcacaa ccatccgtct ggtgtcgccg agcccagccg
     2281 cgccgacgag cttctaacgc agacgctcaa gcaggcgctg gcgctggtcg agatcaggac
     2341 gctcgaccat ttcatcgtcg ccggcacgcg aacggtgtcg tttgccgagc gcgggctgct
     2401 gtaggcattg ccctgccaaa tgcttgaatc ggcttggatt tccggttaca atccaagact
     2461 ttcgcaattt caacgagttg gagcgagcga tggctcgagt ctgtcaggta acgggcaaag
     2521 ccccgatggt gggtcaccac gtgtcccacg ccaacaacaa gaccaagcgc cggtttctgc
     2581 cgaacctgca gcgccgccgt ttctgggtcg aaaccgagaa ccgctgggtg agcctgcggc
     2641 tgaccaatgc cgcgctgcgc acgatcgaca agaacggcat cgacgtcgtc ctggcggaca
     2701 tgcgcgcccg cggcgagcac gtctaagccg gcgcgagctc aaggagattt cagaccatgc
     2761 gtgaaaagat caagctcgaa tcgtcggccg gcaccggtca tttctacacc acgtcgaaga
     2821 acaagaagct gatgccggaa aagatggaga tcaagaagtt cgacccggtc gctcgcaagc
     2881 acgtgatgta caaggaaacg aagctcaagt gatgccggat taaagccgcg cctcgcgcgg
     2941 ttgcacgata aaaaaagccc gccttccggc gggctttttg cattgtgagg agaggactgc
     3001 ttacgcgtag ctgcgcaggg tccgcgaaaa cttctgcagc gcggcgatgt tgcttgcctc
     3061 ggcgcgatga caccagtcct gaaggcgcgc cagcagctgc tcgcgtgaat cgttcgaacg
     3121 ctcccacagt ccggcgagct cctgccgcat agagtagatc gtcttgagtg ccgagctgcc
     3181 ctcgagcgtg gtctcgaggt gcgcgcgatc ggtttcaggc aacgcggtgg cgtccgactg
     3241 gaaccatcgg cgcagccggc ggaagctcgg catgtccggc gtgagctcgc cgcggcgagc
     3301 gcgctcgcgc aagctggcga cctcctgcct gtacgcgagc ttcagcgacg aggcgtagct
     3361 ggtcagcacg gcgtagcggt gggtgatgat cgcctgcagc gtttccaggt cggcgttcaa
     3421 cttgacgcgg tcgaatttga gccgcggcgc gaccttgcgc acggtggcaa ggccgaagaa
     3481 ctccagcgtg cggatgtatg cccagcccag atcgaattca taccagcgcg atgaaagctt
     3541 ggccgacgtg ccgtaggcgt ggtggttgtt gtgcagctct tcgccgccga tcaagatgcc
     3601 ccaggggacg atgttgcggc tcgcgtcctc gcaggagaaa ttgcggtagc cccagtaatg
     3661 cccgatgccg ttgatgatgc ccgccgcggt caccggaatc caggccatct gcaccgccca
     3721 gatcgtcgcg cccagcggac cgaacagcgc gagattgacg accagcatca gcccgacacc
     3781 ttcccaggaa tggcgcgtgt agacgttgcg ctcgatccag tcgtccggtg tgcccagtcc
     3841 gtacttgtcc agcgtttcgc cattcttggc ttcgtcgcgg tagagctctg cgccttccca
     3901 gaagaccttg ttgatgccgc gagtctgcgg gctgtgcggg tcttcggcgg tctcgacctt
     3961 cgcgtggtgc ttgcgatgga tcgcgaccca ttccttggtg accatgccgg tcgtcagcca
     4021 aagccagaag cggaagaagt ggctgaccag cggatggagg tccaggccgc ggtgggcctg
     4081 cgagcgatgc agataaatag ttacggcggc gatggtgatg tgggtgaaca ccagcccgac
     4141 aacgacgtag ccccaccacg gcagcgggac caggccgtgc gcgagcaggg aaagaggatc
     4201 gaaagacatc agtggaacat cctaaaaagg gagggtgggc cgggcggccc aatgtgtggt
     4261 atcggacagt cgacagttta catcgtttca tcgcctatcg cccgaccggc gatcgcccga
     4321 ctgtccagta caagcgatca aagcgggggc gaagtctcgg cattgtcgcc gggcgcttgg
     4381 ccgatgatgc gcacttcgcg ctgcgggtag gggatccgta tgccattgtc gcgaaacgct
     4441 ttccatatgc cgatgttcag cgccgacctc aggttgccct ggcccatttc ggggtcgttg
     4501 atccagacca caagctcgag ggcgatgccg ttgtctccga actggagcac gttgacgatc
     4561 ggcgcgagcg cgccttggag gac
//
LOCUS       DATANS010000262         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_809604, whole genome shotgun sequence.
ACCESSION   DATANS010000262 DATANS010000000
VERSION     DATANS010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809604"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="VHT22_03735"
     CDS             complement(<1..1246)
                     /locus_tag="VHT22_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3136695.1"
                     /translation="MNANLYALLRSHFAEHLEQPCVLIPGGPVIHYDDLDAAAARIAH
                     ALVAAGCIIGDRVAVQTDKCWQVLAVYLACLRAGLVYLPLNTGYRKAELAYFFGDAEP
                     RVIVCAPADAADIAALRTSATVLTLAGGEGSLLDRARDHPAQFETVTSAPGDLAALLY
                     TSGTTGRSKGAMLTHRNLATNALALVEHWGFTRGDVLLHALPLFHVHGLFVACHCALL
                     SAGRMLWLPRFDAAEVARLLPHATVMMGVPTYYSRLLADQSFGTEACRTIRVFISGSA
                     PLLSETFNDFAARTGHAIVERYGMTETGMNTSNPLDGERRCGSVGVALPGVSVRVVGA
                     DGATCAPGEVGDIEVKGPNVFAGYWRMPDRTREEFTDDGYFRTGDVGEMAADRYLRIV
                     GRAKDLIITGGLNVYPKEIEERI"
     gene            complement(1322..>2008)
                     /locus_tag="VHT22_03740"
     CDS             complement(1322..>2008)
                     /locus_tag="VHT22_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX3136696.1"
                     /translation="VAKKMSKDGDKFGTGVLEGVVAPETAAHAAGTSALLPMITLGIP
                     GSPTAAVLLGGLLIWGLQPGPLLFAEKPDFVWGLIASMYLGNVAGLIVVLSLVPLFAG
                     ILRIPFSIIAPVIVAICAIGAYTVNNAMQDIWFMLGFGVIGYLFKKLNYPLAPMVLAL
                     VLGNNAENAFRQAMLMSQGDMTIMWSNWLVGSITTLAVIMLFWPLISRVLKAIVPAKP
                     DKLSEQRPVE"
BASE COUNT          368 a          646 c          672 g          322 t
ORIGIN      
        1 cgatcctttc ctcgatttcc ttcgggtaga cattgaggcc gccggtaatg atcaggtcct
       61 tggcgcggcc gacgattcgc aagtagcggt cggcggccat ctcgccgacg tcgccggtcc
      121 gaaagtagcc gtcgtcggtg aattcctccc gggtgcggtc cggcatccgc cagtagccgg
      181 cgaacacgtt cggtcctttg acctcgatat cgccgacctc gccgggcgca caagtcgcgc
      241 cgtcggcacc cacgacgcgc acgctgacgc cgggcaacgc cacgccgacg ctgccgcagc
      301 ggcgctcgcc gtcgagcgga ttggatgtat tcattccggt ttcggtcatg ccgtaccgct
      361 cgacgatggc gtgtccggtg cgcgcggcaa agtcgttgaa cgtttccgac agcagcggcg
      421 cagaaccgga aataaatacg cgaatcgtgc ggcaggcctc cgtgccgaaa gactggtcgg
      481 caagcaggcg cgagtaatag gtcgggacgc ccatcatcac cgtcgcgtgc ggcaagagcc
      541 ttgccacttc cgcggcgtcg aatctaggca gccacagcat tctgccggcc gacagcagcg
      601 cgcagtggca ggcgacgaac aggccgtgca cgtgaaacag cggcagggca tgcagtaaca
      661 catcgccgcg cgtgaagccc cagtgctcga ccagcgccag tgcgttggtg gccaggttgc
      721 gatgcgtcaa catcgctccc ttcgagcggc ccgtcgtgcc cgaggtgtag agcagcgccg
      781 cgagatcgcc cggggccgag gtcacggtct cgaactgcgc cgggtggtcg cgagctcggt
      841 cgagcaggct gccttcgccg cccgcaaggg tcagcaccgt cgcgctggtg cgcagcgcgg
      901 cgatgtcggc ggcgtcggcg ggagcgcaca cgatgacgcg gggttcggca tcgccaaaga
      961 aataggcgag ctcggctttg cgatacccgg tgttgagcgg caggtacacc agcccggcgc
     1021 gcagacaagc caggtagacc gccagcacct gccagcattt gtcggtctgc acggcgacgc
     1081 gatcgccgat gatgcaaccg gcggcgacca acgcgtgggc gatgcgcgct gcggcggcgt
     1141 cgagatcgtc gtagtggatg accgggccgc ccggaatcaa cacgcagggc tgctcaagat
     1201 gctcggcaaa atggctgcgc agcagcgcgt aaaggtttgc gttcatgttc aagcgtgttc
     1261 gcgatgccgt gcgccgtgac aagcttgcgg cgcgacgcga agcggggcgt tccgacgccg
     1321 gctattccac cggtcgctgc tcggacagct tgtccggctt ggccggaacg atggccttga
     1381 ggaccctgga aatcagcggc cagaagagca tgatcaccgc aagcgtggtg atgctgccca
     1441 ccagccagtt cgaccacatg atagtcatgt cgccctgcga catcagcatc gcctggcgaa
     1501 acgcgttttc ggcgttgttg ccgagcacca gcgccagcac catcggcgcg agcggatagt
     1561 tgagtttctt gaacaggtag ccgatgacgc cgaagccgag catgaaccag atgtcctgca
     1621 tcgcgttatt gaccgtgtag gcgccgatcg cgcagatcgc gacgatcacc ggggcgatga
     1681 tcgagaacgg aatgcgcagg atgccggcga acagcggcac aaggctcagc acgacgatca
     1741 atccggcgac gttgcccagg tacatgctgg cgatcagccc ccagacgaag tcgggttttt
     1801 cggcgaatag cagcgggccc ggctgcaggc cccagatcaa aaggccgccg agcagcaccg
     1861 cggcggtcgg cgagcccggg atcccgagcg tgatcatcgg cagcagcgcg ctggttccgg
     1921 cagcatgcgc cgcggtctcc ggcgcgacga caccctccag cacgccggtg ccgaatttgt
     1981 cgccgtcctt cgacattttc ttggccac
//
LOCUS       DATANS010000263         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_560252, whole genome shotgun sequence.
ACCESSION   DATANS010000263 DATANS010000000
VERSION     DATANS010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560252"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..370
                     /locus_tag="VHT22_03745"
     CDS             203..370
                     /locus_tag="VHT22_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136697.1"
                     /translation="MGTRALKLMVILLCLINAAMWLFYTESALMATLWGAVAVGFFVW
                     IIDDMRRGVPR"
     gene            413..1285
                     /locus_tag="VHT22_03750"
     CDS             413..1285
                     /locus_tag="VHT22_03750"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3136698.1"
                     /translation="MTETATGNDQFTAFWNEVLAAKFERFRNILLDGLSYHSAVPLRT
                     LDLRPGAAALDVGCGWGDTALQLARIVGPQGSVLGLDCCEAFLEKGRRDAVEAGLRNV
                     RFVTADVQTYPFKAEFDFCFSRFGMMFFSNPVAAMRNISRALKPGGRLMFVTWRSVEH
                     NPWAGLPKAVVLKFLPPPGEDAQTCGPGPFSMANTDTVRAQLKAAGFEDIAFHATDGP
                     VMIGSTVEQALQFQLALGPAGEIFREAGDQATSRQREIEAALSSELGRYRQPNGEVVM
                     QSSSWTITARKPSI"
     gene            1289..1777
                     /locus_tag="VHT22_03755"
     CDS             1289..1777
                     /locus_tag="VHT22_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX3136699.1"
                     /translation="MRQFDLTTQWQLESAIDRVWDAIVTVEKWPHWWRFVHAVEELEK
                     GDAEGLGALRTYTWSSKLPYRLTFAMRVTVVRRPTYLEGVAVGDLTGNGRWRLAAEGG
                     TTRVRYAWTVFTTKRWMNALAPLLEPAFRWNHHQVMAEGGRGLAKYLGVRLLANDAAP
                     AR"
     gene            1875..>2249
                     /locus_tag="VHT22_03760"
     CDS             1875..>2249
                     /locus_tag="VHT22_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136700.1"
                     /translation="MSQGVRKVDYFHVMVPNKPGRGAQVLAGLAAERVNLLAFSGFPN
                     GSKGQLDLIPESSAALRRAARKLGLDLSARKTGFLVHGADRVGAMTRLLDTLAAAKIN
                     VTAMDAVTAGAGRYGAIFWVKPK"
BASE COUNT          389 a          698 c          760 g          402 t
ORIGIN      
        1 acgccgcggc gcatcttcgt gccggtgttc atcggcgtcg tgctgatcgc gccatgcatg
       61 gcgacgatct tcctgttcac cgcctgggcg ctggacggaa gcgcgcggta gcaacgcaag
      121 cacggcacgc tttcgctccc cggcagaggc gctgtgacgc cgcctggtag gcgctcgcgc
      181 gcttgcaagt tagaatctcc ccgtgggcac ccgcgcgctg aagttgatgg tcattctgct
      241 ttgcctcatc aacgcggcga tgtggctgtt ctataccgag tcggcgctga tggcgacgct
      301 gtggggtgcg gtcgccgtcg gattttttgt ctggatcatc gacgacatgc gcagaggcgt
      361 accacgctga acgaagaccg gatcggcgcc gacggccgac gcaaggagcg acatgaccga
      421 aaccgcgacc ggcaatgacc aattcactgc cttctggaac gaagtcctcg ccgcgaagtt
      481 cgagcgcttt cgcaatatcc tgctcgacgg gttgagctac cacagcgccg tcccgctgcg
      541 cacgctggac cttcgcccgg gcgcggccgc cctcgacgtc ggctgcggct ggggcgacac
      601 cgcgctgcag ctggcgcgca tcgtcggccc gcaaggatct gtcctcgggc tggactgctg
      661 cgaagctttc ttggagaaag gccgccgcga tgccgttgag gctggattgc gcaacgtgcg
      721 cttcgtcacg gccgatgtgc agacttaccc gttcaaggcg gaattcgact tctgcttttc
      781 gcgtttcggc atgatgttct tcagcaatcc ggtggcggca atgcgcaata tctcgcgggc
      841 gctgaaaccg ggcgggcggc tgatgttcgt cacatggcgg tcggtcgagc acaacccctg
      901 ggcaggcctg ccgaaagccg ttgtgctcaa gttcctgccg cctccgggcg aggacgcgca
      961 gacctgcggc cccggtccat tctcgatggc gaacaccgac accgtcagag cccagctcaa
     1021 ggcggcagga ttcgaggaca ttgcctttca cgcgacggac ggaccggtca tgatcggcag
     1081 cacggtcgag caagcgctgc agtttcagct cgcgctcggt cccgccgggg agatatttcg
     1141 cgaggccggc gatcaagcaa ccagccggca gcgagagatc gaagcggcgc tatcgtccga
     1201 gctgggtcgc tatcgccaac cgaacggcga ggtagtcatg caatcgagct cgtggacgat
     1261 caccgcgcgc aagcccagca tctgatcgat gcggcaattc gacctgacga cgcagtggca
     1321 actcgaatcc gcgatagatc gcgtctggga tgcgatcgtc accgtggaaa agtggccgca
     1381 ctggtggcgt ttcgtgcatg cggtcgagga acttgagaag ggcgacgccg aaggtctagg
     1441 cgcgctgcgc acctacacct ggtcgagcaa gctgccgtat cggctcactt tcgcgatgcg
     1501 cgtgacggtg gtaaggcggc cgacctacct ggaaggtgtg gccgtcggcg acctcaccgg
     1561 caacggccgc tggcgcctcg cggccgaagg cggcacgacc cgcgtccgct acgcttggac
     1621 cgtcttcacg accaagcggt ggatgaatgc gctggctccg ctgctggagc ctgcctttcg
     1681 ctggaatcac caccaggtga tggccgaggg agggcgcggg ctggcgaaat atctcggggt
     1741 tcgcctgctg gcgaatgacg ccgcgcccgc gcgatgatgg ttcggaggct tgcagcgctc
     1801 ggtgcggtct cgttgacgcg gcgcgccgga agattcagag tttcatcgcc tgccgtgcag
     1861 gcaaaggagt caacatgagc caaggtgtgc gcaaagtcga ttactttcac gtgatggtgc
     1921 ccaacaagcc gggacgtggc gcgcaagtgc tggcgggtct cgccgccgaa cgcgtcaacc
     1981 tgctcgcgtt ttccggattt ccaaacggca gcaaagggca gctcgatctg attccggaga
     2041 gcagcgcagc gctgcgccgc gccgcgcgca agctcggtct cgacttgagc gccaggaaga
     2101 ccggcttcct ggtgcatggc gccgaccgcg tcggcgcgat gaccaggctg ctggacacgc
     2161 tggcggcggc gaagatcaac gtcactgcga tggatgcggt tactgcggga gcgggtcgat
     2221 acggcgcgat cttctgggtg aagccgaag
//
LOCUS       DATANS010000264         3368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_560269, whole genome shotgun sequence.
ACCESSION   DATANS010000264 DATANS010000000
VERSION     DATANS010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560269"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1513)
                     /gene="mnmE"
                     /locus_tag="VHT22_03765"
     CDS             complement(152..1513)
                     /gene="mnmE"
                     /locus_tag="VHT22_03765"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX3136701.1"
                     /translation="MPTSPATELIAAIATPAGRGGIGIVRLSGPDLQRVIDGVVGRAL
                     EPRVATSANFVGAHGETLDQGLALYFPAPASYTGETVLELHGHGGPAVLAQILRRCLD
                     LGARLAEPGEFTKRAFLNGKIDLAQAEGVADLIDAATATAARAAARSLTGAFSAEVRA
                     LVDRLTELRMFTEATLDFPDEDIEFMQRADAVGKLATLRERLDSIAARSRQGSLLREG
                     LDVVLIGQPNVGKSSLMNQLAGDDVAIVTPHAGTTRDAIRSSVEIRGIPLHIIDTAGL
                     RESADPIERIGIERTWATIARADLALIVTDARESDQDADVAIVGRLPQSLRLIVVRNK
                     IDLAGIEAKSSASSGAAEVWLSAKTGAGVELLREAMLAAAGAHEDMEGTFLARERHLQ
                     AMRAAGTHLDAAGQHLRTSPPPLELFAEELRGAQQSLSAITGEVSADDLLGVIFSRFC
                     IGK"
     gene            complement(1545..3230)
                     /gene="yidC"
                     /locus_tag="VHT22_03770"
     CDS             complement(1545..3230)
                     /gene="yidC"
                     /locus_tag="VHT22_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX3136702.1"
                     /translation="MDTQRLILFVIFSFSALFLWEAWQREHRPPPPPVTQTAPRSSAE
                     SPVPTATPVPGAAVTANPNAAPAAAPAGETIAIRTDLYGADIDTAGAVITKVALNEHR
                     DTEDTSRPYLLLQKNADRTFVAQSGLLGEGLPNHRTLWQPVPGPRELAPGADTLELKL
                     QASAANGDKVVQTLTFHRGSYVIDVDYAITNAGAAPISPHAYFQFTRDTKSQSQQNSM
                     APSSYIGPVVYNEADKFKKVEFGDLDKLASDPSRKPPFTKNADNGWVGMVEHYFVAAW
                     LPPEEPKTPREFYARKLDNGLYAAGVIVPGPPIAPGATGDIKVKLYAGPQIQDTLKSL
                     AKGLDLVVDYGIFTVIAAPLFVLLKWLHSLVHNWGWAIVLLTIIIKSVFYPLNHASAR
                     SMAKMKVIAPKLKALQEQYSKDKQQLQIKMMEMYKTEKINPLGGCLPILVQIPVFIAL
                     YWVLLSAVELRNAPWILWIKDLSAPDPYFVLPVIYAITAYLQVKLSPTPISDPVQAKV
                     MQIMPIAFSVMFIFFPAGLVLYWLINNSLQIFQQWHMNRVIGRETAAAAAKRR"
     gene            complement(3258..>3368)
                     /locus_tag="VHT22_03775"
     CDS             complement(3258..>3368)
                     /locus_tag="VHT22_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor"
                     /protein_id="HEX3136703.1"
                     /translation="AREAIERHGAARGVWLASRRVARCHPYHAGGYDPVP"
BASE COUNT          563 a         1072 c         1156 g          577 t
ORIGIN      
        1 cgccgcagaa tcggtcaggc cactgggctg ccggtccggt ccctgcggtg acgccgaccg
       61 gagcaaccac gaaaacagcg gcctggcgcg ggtcaaggcg aagcgacggc cggcccgccg
      121 ccgcttaccg acggcagccg cataaaggcg cttatttgcc gatgcagaac cggctgaaga
      181 tcacaccgag caggtcgtcg gcgctgactt cgccggtgat cgccgaaagc gactgctgcg
      241 cgccgcggag ctcctcggcg aagagttcga gcggcggcgg cgaggtacgc aggtgctgcc
      301 ccgccgcatc gaggtgcgtt ccggccgcgc gcatcgcctg caaatgccgc tcgcgtgcga
      361 gaaacgtgcc ctccatgtcc tcgtgcgcgc cggctgccgc aagcatggcc tcgcgaagga
      421 gctcgacgcc ggcgccggtc ttggccgaaa gccatacctc tgctgcgccg gaagatgccg
      481 agctttttgc ttcgatcccg gcgagatcga tcttgttgcg aacgacgatc agccgcagcg
      541 actgcggcag tcgcccgacg atcgcgacgt cggcgtcctg gtcggactcg cgggcgtcgg
      601 tgacgatcag cgccaggtcg gcgcgggcga tcgtcgccca ggttctctcg atgccgatgc
      661 gctcgatcgg gtcggcgctc tcgcgcaggc ccgcggtgtc gatgatgtgc agcgggatgc
      721 cgcggatttc gacactgctg cgaatcgcat cgcgcgtcgt gccggcatgc ggagtcacga
      781 tcgcgacgtc gtcaccggcg agctggttca tcaggctcga tttgccgacg ttgggctggc
      841 cgatcagcac gacgtcgagg ccctcgcgca agaggcttcc ctggcgagag cgtgccgcaa
      901 tcgaatcgag gcgctcgcgc agggtggcaa gcttgccgac ggcgtcggcc cgctgcatga
      961 actcgatgtc ctcgtcggga aaatccagcg tcgcttcggt gaacatccgg agctccgtca
     1021 accgatcgac caacgcgcgg acctcggcgg aaaacgcccc ggtcaagctg cgcgccgcgg
     1081 cgcgcgcagc ggtcgccgtc gctgcgtcga tgaggtcagc gacgccctcg gcttgtgcca
     1141 gatcgatctt gccgttgagg aacgcccgct tggtgaattc gccgggctcg gcgagccgtg
     1201 cgccgaggtc aaggcagcgg cgcaatattt gcgccaacac cgcgggacca ccgtgtccgt
     1261 gcaattcgag gaccgtctcg ccggtgtacg aggccggcgc cggaaaatac agcgccagtc
     1321 cctggtcgag cgtttcgcca tgcgcgccga caaagttggc gctggtcgcg acgcgaggct
     1381 cgagcgcccg gccgacgacg ccgtcgatga cgcgctgcag gtccgggccc gacaggcgca
     1441 cgatgccgat gccgccgcga ccggcgggcg tcgcgatcgc ggcgatcagc tcggtagccg
     1501 gcgacgtcgg catgctttgc ccgcgcgcgg ggcttcgagt cgtgctagcg gcgcttcgct
     1561 gccgcggcgg cggtctcgcg cccgatcacg cgattcatat gccactgctg gaaaatctgc
     1621 agcgaattgt tgatcagcca gtacagcacc aggcctgccg ggaagaagat gaacatcacc
     1681 gagaacgcga tcggcatgat ctgcatcacc ttcgcctgca cgggatcgga gatcggcgtc
     1741 ggcgagagct tcacctgcag gtaggcggtg atcgcgtaga tcaccggcag cacgaaatac
     1801 gggtccggcg cggagagatc cttgatccac agaatccacg gggcgttgcg cagctccacg
     1861 gcggagagca gcacccaata gagcgcgatg aacaccggga tctgcaccag gataggcaga
     1921 catccgccga gaggattgat cttctcggtc ttgtacatct ccatcatctt gatctgcagc
     1981 tgctgcttgt ccttggaata ctgctcctgc agcgctttga gtttcggagc gatcactttc
     2041 atcttggcca tcgagcgcgc gctggcgtgg ttcagcggat aaaaaacgct tttgatgatg
     2101 atcgtcagca acacgatcgc ccagccccag ttgtgcacca gcgagtgcag ccacttgagc
     2161 agcacgaaca gcggggccgc gatgaccgtg aatatcccgt aatcgacgac gaggtcgaga
     2221 cccttggcca gcgatttcag cgtgtcctgg atctggggcc cggcgtagag cttgaccttg
     2281 atgtcgccag tcgcgccggg tgcgatcggc gggccgggga cgatcactcc tgccgcgtaa
     2341 aggccgttgt cgagcttgcg cgcataaaac tcgcgcggcg tcttcggctc ctcgggagga
     2401 agccacgcgg cgacgaagta gtgctcgacc atccccaccc agccgttgtc ggcattcttg
     2461 gtgaacggcg gcttgcggct cggatcgctg gcgagcttgt ccaggtcgcc gaattcgact
     2521 ttcttgaatt tgtcggcctc gttgtaaacg accggtccga tgtacgacga gggcgccatc
     2581 gagttctgct ggctctgcga cttagtatcc cgggtgaact ggaaataggc gtgcggcgag
     2641 atcggcgccg cgcccgcatt ggtgatcgca tagtcgacgt cgatcacgta gctgccgcgg
     2701 tggaaggtca gcgtctgcac aaccttgtcg ccgttggcgg cgctcgcctg cagcttgagt
     2761 tccagcgtgt ctgcgccggg cgccagctct cgcggcccgg gcacgggctg ccacaacgtg
     2821 cggtgattgg gcagtccttc gccgagcagg cccgattgcg caacgaacgt gcgatcggca
     2881 tttttctgca aaagcagata cggccgggaa gtgtcctcgg tgtcgcggtg ctcgttcaac
     2941 gcgaccttgg tgatgaccgc gccggcggtg tcgatgtcgg cgccgtagag gtcggtgcgg
     3001 atcgcgatcg tttcgccggc cggcgctgcg gcgggtgcgg cgttcgggtt cgccgtgacg
     3061 gcagccccgg gtaccggcgt ggccgtgggt accggcgatt cggcggacga gcggggcgca
     3121 gtctgcgtca ccggcggtgg cggcggccgg tgctcgcgct gccacgcctc ccacaggaag
     3181 agcgcggaga acgaaaagat gacaaaaaga atcaggcgtt gggtgtccat gactgccgaa
     3241 atcgaatcgt gatggtgcta gggaaccgga tcgtagccgc cggcatgata cgggtggcag
     3301 cgcgccaccc gtcgcgaggc aagccacacg ccgcgcgccg cgccgtggcg ctcgatggcc
     3361 tcgcgcgc
//
LOCUS       DATANS010000265         9533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_308167, whole genome shotgun sequence.
ACCESSION   DATANS010000265 DATANS010000000
VERSION     DATANS010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 9533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9533
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308167"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..431)
                     /gene="rnpA"
                     /locus_tag="VHT22_03780"
     CDS             complement(78..431)
                     /gene="rnpA"
                     /locus_tag="VHT22_03780"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX3136704.1"
                     /translation="MPIGSNQRHRLRGAAAFTRLFRQGRRIEAEYLQLLAVPAGGPIG
                     QVGYVIGKQQLKLAVDRNRLKRMLREAVRLRRPGLDRFDIVLRLRRGCPPARIAGLGT
                     EAARLLDALTAARSQ"
     gene            complement(443..577)
                     /gene="rpmH"
                     /locus_tag="VHT22_03785"
     CDS             complement(443..577)
                     /gene="rpmH"
                     /locus_tag="VHT22_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX3136705.1"
                     /translation="MKRTYQPSVIRRKRTHGFRARMKSIGGRKVLRARRAKGRARLAV
                     "
     gene            756..2090
                     /gene="dnaA"
                     /locus_tag="VHT22_03790"
     CDS             756..2090
                     /gene="dnaA"
                     /locus_tag="VHT22_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017510174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX3136706.1"
                     /translation="MSTPVFWQACLESFQQELTQQQFNTWIRPLRLEIAGDELRLIAP
                     NRFVLQWVKERFAGRIEELAAVAVGRPLRLGLVVADGAERVPAPAVTAISPDPGAAAT
                     RRGEQASVNPAFTFSTFVAGKANQLARAAGMQVADNPTSYNPLFVYGGVGLGKTHLIQ
                     AIGNQILANDPAAKIRYIHAETYVSDVVRAYQHKAFDDFKRYYRTLDLLLIDDIQFFN
                     GKSRTQEEFFYLFNTLIEARKQVVITCDTYPKEISGIEERLISRFGWGLTVALEPPEL
                     EMRVAILLSKAAQTGMKLDEQVAFFIAKHIRSNVRELEGALKRVLAYSSFHRQEISLS
                     VAKDALRDLLAVQNRQISIENIQKTVADYYKIRISDMHSKKRSRAIARPRQVAMALAK
                     ELTQLSLPEIGSNFGGRDHTTVLHACRQIAKLRESVPEMTHDVNFLLQVLRN"
     gene            2329..3432
                     /gene="dnaN"
                     /locus_tag="VHT22_03795"
     CDS             2329..3432
                     /gene="dnaN"
                     /locus_tag="VHT22_03795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX3136707.1"
                     /translation="MQLKQTPRDALLKPLQAVSGIVERRHTLPILANVLLEQKDGRLF
                     VTATDLEMQITAHSELAGKQDQAVTVAARKLQDLLRALPDGATVNIETSASKMTVRAA
                     RSRFNLQTLPASDYPRISIAQEQQQAITLPQRQFRGLLKLAEFAMAQQDIRYYLNGML
                     LVVDKGSLQAVATDGHRLSYASITVDGDYARQEVILPRKTVLELGKLLEDSDAEVKID
                     LLANQVRFRFGNIELVSKVVDGKFPDYNRVIPVGHAKQIELARSELLQALQRAAILSN
                     EKFRGVRLVLGNGQLKIICTNSEQEEAEEELEVGYQGETLDIGFNITYLLDVLHNLGT
                     DKVVLAFGDANSSALVTMPERNDYKYVVMPMRI"
     gene            3521..5944
                     /gene="gyrB"
                     /locus_tag="VHT22_03800"
     CDS             3521..5944
                     /gene="gyrB"
                     /locus_tag="VHT22_03800"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX3136708.1"
                     /translation="MTQANPKPTDNGSDYDSTSIQVLKGLDAVRKRPGMYIGDTSDGT
                     GLHHMVFEVLDNAIDEALAGYCDDIKVVIHADNSVSVVDNGRGIPTDIKEDDELKRSA
                     AEIVMTELHAGGKFDQNSYKVSGGLHGVGVSVVNALSVWLKLRIWRDGKAHQIEFHDG
                     VPVAPLAVIGPTERRGTEVHFMAAATTFDHIEFHYDILARRLRELSFLNHGVKIELVD
                     QRDGKSENFAFTGGIKGFVEYMNRSKTVLHPKVFHAVGEKDGVIVEVAMQWNDSYAES
                     VQCFTNNIPQRDGGTHLTGLRQAMTRTLNSYIETEELAKKAKVETTGDDMREGLTCVL
                     SVKVPEPKFSSQTKDKLVSSEVQPLVQEIVGGKLHEFLLENPTDAKIICGKIIDAARA
                     REAARKARELTRRKGVLDGMGLPGKLADCQERDPALCELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKILNVERARFDKLISSQEIVTLITALGTGFGKDEYNADKLRYHRIIIMT
                     DADVDGSHIRTLLLTFFYRQMLDLVERGHIYIAQPPLYKAKQGKEETYLKDDHDLKQH
                     LLKVALKGAELVPGAELAPLSIEALDHIARQYLLAEAVIDRVARLVDPAALHALLTGL
                     AIDLSTADAAAASAAALERAIGDPDITVQARYDASAESRRLVIVHTRHGTPHATSLDA
                     DFLVSGDYAQIRAAAEVLRGLIGPGASVRRGERSSAVASFKEALDWLLGEARGAVSIQ
                     RYKGLGEMNPAQLWETTMDPAVRRLLKVQIEDGIASDEIFTTLMGEDVEPRRAFIENN
                     ALGVRNLDV"
     gene            5954..8179
                     /locus_tag="VHT22_03805"
     CDS             5954..8179
                     /locus_tag="VHT22_03805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX3136709.1"
                     /translation="MIVRATLACLLTLAALAPASPASISGLPEPTTQRLIVKFRSPMP
                     GAGVETESVRGAGRVSRLAADSGVALAYVRPMALGAHVVAFDHPVPLSEARAIARRLA
                     GRAELEYVQPDHRMRAQFVPNDQYVSLQTYLRDDAAGISAFSAWDVATGSANIVVAVV
                     DTGYRPHAAMAGRILPGYDFVSDPIVANDGDGRDADASDPGDWITRTDLTSSHTFDGC
                     DIENSSWHGTSTAGIIAANSNDAVWTAGIDWAAKILPVRVLGKCFGDDSDIIDGIAWA
                     AGLSVPGVPANPTPAQVINLSIGEPAECAPGYQQVIASALAHGITRAIVASAGNDGAD
                     VTTNTPANCPGVISVGATTSAGSRASYSNFGATISAPGGDTFGRFDGIEILSNLGHSI
                     PTTDGLDVGNGTSYAAPMVSGVISLMLSAAPGLSASQVRAVLTSSAKPFPPGSDCTTA
                     TCGAGIVDAYAAVQAAQGIEELSVNFGGLWWNAPGGSESGWGINFAHQGDQIFASWFT
                     YDLNGKAWWLVMSANRIAHQVYRGTLFQGTGPSFDTVPFPPLGTPGGAAGSPVGVATL
                     TFSDSNNAAFSYTVNGIAQTKSITREAFGLLPTCLFAAQVNLALTRNYQDLWWAAPAA
                     SEAGWGINIAHEGDTIFATWYTYDRDGSPMWLVTTADKSGLSSYSGDLIRLTNGPPFN
                     AVPFPPIGSPGGATGSVVGTATLSFFDGNSGIFSYSVDDVVQTKNITREVFTSPGTVC
                     H"
     gene            complement(8225..8830)
                     /locus_tag="VHT22_03810"
     CDS             complement(8225..8830)
                     /locus_tag="VHT22_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEX3136710.1"
                     /translation="MAQFRITLVGSRYFGAATLARLLEESVDIGRVLVPAADDRLALA
                     AASAGLEVKVLDDPKLVPADAIAEGTDLIVAAHSHARVSNEALARSRLGGIGFHPSLL
                     PRHRGIAAVEWTIREADSIAGGSIYHLADRMDAGAIAAQDWCFVKKGETARELWERAL
                     APMGLALMARVIRHAKEHGTIPAQPQDEQFATQAPLIRDGA"
     gene            complement(8836..>9533)
                     /locus_tag="VHT22_03815"
     CDS             complement(8836..>9533)
                     /locus_tag="VHT22_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HEX3136711.1"
                     /translation="GDGAEGEAPDFSDQEPLGACPKCGSRVFALPAAYVCEKSVGPDK
                     TCDFRSGRTILQRPIESEQMRKLLATGKTDLLQFVSSRTRRPFAAYLVRQPDGKIGFE
                     FEARKDGAGKPLRGGRGAAALRVLGKHPKDKQPVELHAGRYGPYVKHGGVNATLPDRD
                     KIDALTLDEAVALLAEKSGKGASVRRAKTPKNSRPAAVSTKRPGATSAKPLAKSPRTA
                     KASPRAATRKRAT"
BASE COUNT         1739 a         3103 c         3055 g         1636 t
ORIGIN      
        1 atagaaacgg cagttcgcac cgagcagcgg acgcagcgcg tattggtatg cccgcagcag
       61 gccgagcagc accgttttca ttgcgacctt gccgcggtca gcgcgtccag cagccgcgct
      121 gcctcggtgc cgagccccgc gatgcgcgcc ggcggacagc cgcgtcgaag ccgcagcacg
      181 atgtcgaatc gatcgagccc cgggcggcgc aggcgcaccg cttcgcgcag catgcgtttg
      241 agccgattgc ggtcgaccgc gagcttcagt tgttgcttgc cgatcacgta tcctacctgg
      301 ccgatcgggc cgccggccgg caccgccagc agttgcagat attcggcttc gatgcgacgg
      361 ccttggcgaa atagcctggt gaacgccgcc gccccacgca atcggtgccg ttgattcgag
      421 cctatcggga ttcgcgacga ctctagacgg cgagccgggc gcggcccttg gcgcgccggg
      481 cgcgcaggac cttgcggccg ccgatgctct tcatgcgggc gcgaaagccg tgcgtccgct
      541 tgcgccggat gaccgacggc tggtaggtgc gcttcatgat ctgtttccgt tcgaaaaaag
      601 ccttacatta gaacgttttg catagcgccc tgtcaaggta atccgcgtcg atcgctgtgg
      661 ataactttgc agcaagcgag tacaatggcg ctgtcacggg ctccgttcgg cgcccgctat
      721 tttttggtct tgccttcccg cacgcactat ccgccatgtc cacgcccgta ttctggcagg
      781 cctgcctgga atcgtttcag caggagctga cgcagcagca attcaatacc tggattcgtc
      841 cgctgcggct ggaaattgca ggcgatgagt tgcggctgat cgcccccaac cggtttgtac
      901 tgcagtgggt caaggagcgg tttgccgggc gcatcgagga gctggccgcg gtcgcggtcg
      961 gccgaccgtt gcgactggga ctggtcgttg ccgacggcgc cgagcgcgtg cctgccccgg
     1021 ctgtcaccgc gatctcacct gaccccggtg ccgcagcgac gcgacgcggc gagcaggcca
     1081 gcgtcaatcc tgcgttcacc ttctccactt tcgtcgccgg caaggccaac cagctcgcgc
     1141 gcgcggcggg catgcaggtc gccgacaatc cgacttcgta caaccccctg ttcgtttatg
     1201 gcggcgtcgg gctcgggaag acacacctga tccaggcgat cggcaaccag atcctcgcca
     1261 acgatccggc cgcgaagatc cgctacattc atgccgaaac ctatgtctcc gacgtcgtgc
     1321 gcgcctatca gcacaaggcg ttcgatgatt tcaagcgcta ttaccggacg ttggatctcc
     1381 tgctgatcga cgacatccag ttcttcaacg gcaagagtcg cacgcaggaa gagttcttct
     1441 acctattcaa tacactgatc gaagcgcgca agcaggtcgt catcacctgc gacacctacc
     1501 cgaaggaaat ctcgggtatc gaggagcggc tgatttcgcg cttcggctgg ggtctgacgg
     1561 tcgccctcga gccgccggag ctggagatgc gcgtcgcgat cctgctgtcc aaggcggcgc
     1621 agaccgggat gaagctcgac gagcaagtcg cgttcttcat cgccaagcac atacgctcga
     1681 acgtccggga gctcgaaggc gcgctgaaac gggtgctcgc ctactcgagc ttccaccgcc
     1741 aggaaatctc gctgtcggtg gccaaggacg cgctgcgcga cctgctggcc gtccagaacc
     1801 gccagatctc gatcgagaac atccagaaga cggtcgccga ctactacaag atccggattt
     1861 cggacatgca ttcgaagaag cgctcgcgcg cgatcgcgcg gccgcggcag gttgcgatgg
     1921 ctctcgccaa ggagctgacg cagctgtctc tgccggagat cggcagcaat ttcggcggcc
     1981 gggatcacac gacggtgctg cacgcctgcc ggcagatcgc caagctgcgc gagtcggtcc
     2041 ccgagatgac ccacgatgtc aattttctgc ttcaggtatt acggaactag acgcgcatac
     2101 gaagcgcgac cgggacgtcc ggactgtatg aatcggcact gaacgggttt ataatttgtt
     2161 gattaaccga cccagcccgg cgcgcgcaaa tttgccccca aaccatccac acgccgaaca
     2221 gaacttatac cgcggcgcca gcgcgccaaa gctgcggaat cggaagcctt tttctacgtt
     2281 gtaccgaaac tcgacgaact cctattagct gttattgggt ttaaaacaat gcaactaaaa
     2341 cagacaccca gagacgcgct gctcaaaccc ctgcaggccg tatccggaat agtcgagcgc
     2401 cgacatacac tgccgatact cgccaacgtg ctgctcgaac agaaggatgg tcgcctgttc
     2461 gttactgcca ccgatctcga aatgcagatc accgcgcaca gcgagcttgc cggcaagcag
     2521 gatcaggcgg tcaccgtcgc ggcgcggaag ttgcaggacc tgcttcgcgc gctgcccgac
     2581 ggtgccacgg tcaacatcga gacctcggcc agcaagatga ccgtgcgcgc cgcgcgctca
     2641 cgtttcaacc tgcagacgct gcccgcgtcc gactatccgc gcatcagcat cgcgcaggag
     2701 cagcagcagg cgatcacgtt gccgcagagg cagttccgcg gcttgctcaa gctggccgaa
     2761 ttcgcgatgg cccagcagga catacgctat tacctgaacg gcatgctgct ggtggtcgac
     2821 aagggctctc tgcaggcggt tgcaaccgac ggacaccgcc tttcatacgc aagcattacc
     2881 gtcgacggcg actacgcgcg acaggaagtg atcctgcccc gcaagaccgt gctcgagctg
     2941 ggcaagctgc tcgaggacag cgatgccgaa gtgaagatcg atctgctcgc caaccaggtc
     3001 cgcttccgtt tcggcaacat cgagctcgtc agcaaagtgg tcgacggcaa gtttcccgac
     3061 tacaaccggg tgatcccggt cggacacgcc aagcagatcg aattggcgcg ctccgagctg
     3121 ttgcaggcgc tgcagcgcgc cgcgatcctg tccaacgaga aattccgcgg cgtcaggctg
     3181 gttctgggca acggccagtt gaaaatcata tgcacgaaca gcgagcagga ggaggccgag
     3241 gaagagctcg aagtcggata ccagggtgaa acgctggaca tcggcttcaa catcacttat
     3301 ctgctcgacg tcttgcacaa tctcggcacc gacaaggtcg tgctggcatt cggcgatgcc
     3361 aattccagcg cactggtcac gatgccggag agaaacgatt acaagtatgt cgtgatgccg
     3421 atgcgcatct gaccgccgca acagtgacca tggccacccg cggcgccgac atcagtcggc
     3481 gtcggcgttt tcgagggcgg tcagaaacga gaatccgcga atgacccaag ccaatcccaa
     3541 gcccacagac aacggcagcg actacgattc gaccagcatt caggtcctca aaggccttga
     3601 cgcggtgcgc aagcgtcccg ggatgtacat cggtgacacg tccgacggca ccgggctgca
     3661 tcacatggtg ttcgaggtgc tcgacaatgc gattgacgaa gcgctggccg gctattgcga
     3721 cgacatcaag gtcgtgatcc acgccgacaa ttcggtctcg gtcgtcgaca acggccgcgg
     3781 cattccaacc gacatcaagg aagatgacga gctgaagcgc tcggccgcag agatcgtgat
     3841 gaccgagctt cacgccggcg gcaagttcga tcagaactcc tacaaggtct ccggcggcct
     3901 gcacggtgtc ggcgtctccg tcgtcaacgc cttgtcggtc tggctcaagc tgcgcatctg
     3961 gcgcgacggc aaggcgcacc agatcgagtt ccacgacggc gttcccgtcg cgccgctcgc
     4021 cgtcatcggc ccaaccgagc ggcgcggcac cgaagtgcat ttcatggcgg cggcgacgac
     4081 attcgatcat atcgagttcc actacgacat cctggcgcgc cgcttgcgcg agctgtcgtt
     4141 cctgaatcac ggcgtgaaga tcgagctcgt cgatcagcgc gacggcaaga gcgagaactt
     4201 cgcgttcacc ggaggcatca agggcttcgt cgagtacatg aatcgcagca agacggtgct
     4261 gcacccgaag gtcttccatg ccgtcggcga aaaggacggc gtcatcgtcg aagtcgcgat
     4321 gcaatggaac gactcgtatg cggagtcggt gcaatgcttc acgaacaaca tcccgcaacg
     4381 cgacggcggc acgcacctga ccggactgcg gcaggcgatg acccggacgc tgaacagcta
     4441 tatcgagacg gaagagctgg cgaagaaggc gaaggtcgag accaccggcg acgacatgcg
     4501 ggaagggctt acctgcgtgc tgtcggtcaa ggtgcccgag cccaagttct cgtcgcagac
     4561 caaggacaag ctcgtctcct cggaagtgca gccgctggtg caggaaatcg tcggcggcaa
     4621 gctgcacgag ttcctgctcg agaacccgac cgatgccaag atcatctgcg gcaagatcat
     4681 cgacgctgcg cgagcccgcg aggcggcgcg caaggcacgc gagctgacgc gccgcaaggg
     4741 cgtcctcgac ggcatgggct tgccgggcaa gctcgccgat tgccaggaac gcgaccccgc
     4801 gctgtgcgag ctttacctgg tcgaaggcga ttcggcgggc ggctcggcga agcagggccg
     4861 ggaccgcaag ttccaggcga tcctgccgct gcgcggaaag atcctcaacg tcgagcgagc
     4921 gcgtttcgac aagctgatca gctcgcagga aatcgtgacg ctgatcaccg cgctcggcac
     4981 cggcttcggc aaggacgaat acaacgccga caagctgcgc tatcaccgca tcatcatcat
     5041 gaccgacgcg gacgtcgacg gctcgcacat ccgcacgctg ctgctgacgt tcttctatcg
     5101 gcagatgctc gacctggtcg agcgcgggca catctacatc gcgcagccgc cgctgtacaa
     5161 agccaagcaa ggcaaggaag agacttacct caaggacgat cacgacctta agcagcacct
     5221 gctgaaggtc gcgctgaagg gtgccgagct ggtgcctggc gccgagctcg cgccgctttc
     5281 gatcgaggcg ctcgatcaca tcgcgcgtca gtatctgttg gctgaagcgg tgatcgaccg
     5341 ggtcgcgcgg ctggtcgacc cggcggcgct gcatgcgttg ctgaccggtc ttgcgatcga
     5401 cctgtcgact gcggatgctg cagcggcgag cgcagcagcg ctcgagcggg cgatcggcga
     5461 tcccgacatc accgtgcagg cacgctacga cgcgtcggcg gaatcgcgcc gcctcgttat
     5521 cgtacacacg cggcacggca cgccgcacgc tacgtcgctc gatgcggact tcctggtgag
     5581 cggcgactat gcgcagataa gggcggcggc cgaggtcttg cgcgggctga tcgggcccgg
     5641 ggcgagcgtc cggcgcggcg agcgaagttc cgccgtcgcc tcgttcaagg aagcgctcga
     5701 ctggctgttg ggcgaagctc gcggcgcagt ttcgatacag cgctacaagg ggctcggcga
     5761 aatgaatccc gcgcagctgt gggaaacgac gatggacccg gcggtgcggc ggctgctcaa
     5821 ggtgcagatc gaggatggca tcgcatcgga cgagattttt acgaccttga tgggagagga
     5881 cgtcgagccg cgccgcgcat tcatcgagaa caatgctcta ggcgtgcgca atctcgacgt
     5941 ctgacgcgcg ctcgtgattg tccgcgcgac acttgcctgc ctgctgacgc tggccgcgct
     6001 cgcgcccgcg tcgccagctt ccatctccgg gctgccggag ccgacgactc agcgtctgat
     6061 cgtcaaattc cgttcgccga tgcccggcgc cggcgtcgaa acagaatcgg tgcgcggcgc
     6121 tggccgcgtg tcacggctgg ctgccgacag cggcgttgcg ttggcctacg tgagaccgat
     6181 ggcgctcggt gcgcacgtcg ttgcgttcga ccatccggtg ccgctttccg aggcccgcgc
     6241 catcgcgcga cgcctcgccg gccgcgccga actcgagtac gtgcagccgg atcaccgcat
     6301 gcgggcgcaa ttcgtgccga acgatcagta tgtgagtctg cagacctatc tgcgcgacga
     6361 cgcggcgggc ataagcgctt tcagcgcctg ggatgtcgca accggcagcg ccaatatcgt
     6421 cgtcgcggtc gtcgataccg gctaccgccc tcatgcagcg atggccgggc gcattcttcc
     6481 tggatacgat ttcgtttccg atccgatcgt cgccaacgat ggcgacgggc gcgatgccga
     6541 cgcgtccgat cccggcgact ggatcacgcg aaccgatctg accagctcgc acacgttcga
     6601 cggctgtgac atcgagaaca gcagctggca tggcacatcg accgcgggga tcatcgcggc
     6661 caacagcaac gatgccgtct ggaccgccgg catcgactgg gcggcgaaga ttctgccggt
     6721 gcgcgtgctt ggcaagtgtt tcggcgacga ttccgacatc atcgacggta tcgcctgggc
     6781 ggcgggattg agcgtacccg gtgtccccgc caatccgacc ccggcgcagg tcatcaattt
     6841 gagtatcggc gagccggccg aatgtgcgcc cggctaccag caggtgatcg cgtcggcgct
     6901 ggcgcacggg atcacgcggg cgatcgtcgc gtcggcgggc aacgacggcg ccgacgtcac
     6961 caccaatacg cctgccaact gcccgggcgt catttcggtt ggcgcgacca cttccgccgg
     7021 cagcagagcg tcgtacagca acttcggcgc cacgatcagc gcgcccggag gcgatacgtt
     7081 cgggcggttc gacggcatcg aaatcctaag caaccttggg cactcgatcc cgaccaccga
     7141 tgggctcgac gtgggcaatg gcacgagcta cgccgcgccg atggtgtcgg gcgtcatttc
     7201 gctgatgctg tcggcggcgc cgggactcag cgcaagccag gtgcgggccg tccttacgtc
     7261 gagcgccaag ccgtttcctc ccggctccga ctgcacgacc gcgacctgcg gcgcgggcat
     7321 cgtcgatgcg tatgcggcag tgcaggctgc gcaaggcatc gaagagttgt cggtaaattt
     7381 cggcggcttg tggtggaacg cgcccggcgg gtccgaatcc ggctggggca tcaatttcgc
     7441 gcaccagggc gatcagatct tcgcttcgtg gttcacctac gatctgaacg gcaaagcatg
     7501 gtggctggtg atgagcgcga atcggatcgc gcaccaggtc taccgcggga cactgttcca
     7561 ggggacgggg ccgtcgttcg acacggtgcc gtttccgccg ctggggaccc cgggcggcgc
     7621 agccggctcg ccggtcggcg tcgccacgct gacgttcagc gattcgaaca acgcggcgtt
     7681 ctcctatacg gtcaacggca tcgcgcagac caagtcgatt acgcgcgaag ccttcggact
     7741 gctgccgacc tgcctgttcg cggcgcaggt caatctggcg ctgactcgca actaccagga
     7801 cctgtggtgg gccgcgccgg cagcctcgga ggcgggctgg ggaatcaata tcgcgcacga
     7861 gggcgacacc attttcgcaa cctggtacac ctacgacagg gacggctccc cgatgtggct
     7921 ggtgacgact gcggacaaga gcggattgag ctcgtacagc ggcgacctga tccggctcac
     7981 caatgggccg ccgttcaacg cggtcccgtt cccgccgatc ggcagcccgg gcggcgcgac
     8041 cggcagtgtc gtcggcacag ctacgctgag tttcttcgat ggcaactcgg ggatcttcag
     8101 ctacagcgtc gacgacgtcg tccagaccaa gaacatcacg cgcgaagtct tcacttcgcc
     8161 ggggaccgtc tgccactgaa gtaacgcgcc gcgcgccgcg cggccgggga cgagcggact
     8221 gtcgctaggc gccgtcgcga atcaggggcg cctgcgtggc gaattgctcg tcctgcggct
     8281 gtgccggaat cgtgccgtgc tccttggcgt gccggatcac ccgcgccatc agcgccagac
     8341 ccatcggggc cagcgcgcgc tcccagagct cgcgcgcggt ctcgcctttc ttgacgaagc
     8401 accagtcctg cgccgcgatc gcgcccgcgt ccatccggtc ggccagatga tagatcgagc
     8461 ccccggcgat gctgtcggct tcgcgtatcg tccattcgac ggcggcgatg ccgcgatggc
     8521 gcggcagcag tgacgggtgg aacccgatgc cgcccaggcg tgagcgcgca agcgcctcgt
     8581 tgctgacgcg cgcgtggctg tgcgcggcga cgatcaggtc cgtcccttcg gcgatagcgt
     8641 cggccggaac gagctttgga tcgtcgagga ccttgacctc gagcccggcg ctcgcggctg
     8701 ccagcgcgag cctgtcgtcg gcggccggga ccaggactcg gccgatgtcg acgctttctt
     8761 cgagcaggcg agcgagcgtg gccgcgccga agtagcgcga gccgaccagg gtgatgcgaa
     8821 attgagccaa ggtgttcagg tcgcgcgctt gcgcgtcgcg gcgcgcggcg atgccttcgc
     8881 cgtccggggt gacttggcca ggggcttcgc gcttgttgcc cccgggcgct tggtgctgac
     8941 cgccgccggc cgcgaattct tgggagtctt ggcgcgtcgc acgctggcgc ccttccccga
     9001 cttctcggcc agcagcgcga cggcctcgtc gagcgtgagc gcatcgatct tgtcacggtc
     9061 cggcagcgtc gcgttgacgc cgccgtgctt gacgtagggt ccgtagcggc ccgcgtgcaa
     9121 ctccaccggc tgcttgtcct tcgggtgctt gccgagcacc cgaagcgccg ccgcgccacg
     9181 gccgccgcgc agcggcttgc ccgcgccatc cttgcgggct tcgaattcga atccgatctt
     9241 gccgtccggt tgccgcacca ggtacgcggc gaaaggacgg cgcgtgcgcg aagacacgaa
     9301 ctgcaacaga tcggttttcc cggtcgccag cagctttcgc atctgctcgc tctcgatcgg
     9361 ccgctgcagg atggtgcggc cggaacgaaa atcgcacgtc ttgtcaggtc ccaccgattt
     9421 ctcgcacacg tacgccgccg gcagcgcgaa cacgcgcgag ccgcatttcg ggcacgcgcc
     9481 cagcggttcc tggtcgctga aatcgggcgc ttcaccctct gcaccatccc cgg
//
LOCUS       DATANS010000266         1659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_685708, whole genome shotgun sequence.
ACCESSION   DATANS010000266 DATANS010000000
VERSION     DATANS010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685708"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VHT22_03820"
     CDS             complement(<1..1035)
                     /locus_tag="VHT22_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX3136712.1"
                     /translation="MPKVLILGAGKIGALISGLLAESGSYEVHLADVSGEAANAVVNA
                     HRFPNLHAHKLDATDTAALDAHLKSHPVDAVISGLPYYCNIAVAEAARRAGTSYFDLT
                     EDVEVTRAVRKIAAGAKQAFVPQCGLAPGFISIAANELIGHFDELRTVKLRVGALPQH
                     PNNVLKYSLTWSTEGLINEYGNPCEAIVDGRRIEAAPLEGLEEIEIDGTLYEAFNTSG
                     GLGSLAETYGARTQVMDYKTMRYPGHCEQMRLLMNDLKLNHDRGTLKRVLENAVPQTL
                     QDVVVIYVAVTGKQDGDLREENYVNKVYPQMIAGRLWSAIQVTTAAGITAVVDLVLNS
                     GGRHAGFVRQE"
     gene            1099..>1659
                     /locus_tag="VHT22_03825"
     CDS             1099..>1659
                     /locus_tag="VHT22_03825"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX3136713.1"
                     /translation="MTRSPDLIQWHPASWQSKPAQQQAAYTDTAALQRAVASLARLPP
                     LVVSWEIEALRDHIAAAQRGERFILQGGDCAETFEDCESDKIAKKLKILLQMSLVLVY
                     GLRKPVVRIGRMAGQYAKPRSADTETRDGQTLPSYRGDIINHPGFTPDERDPDPELLL
                     RGYERAALTLNFVRALIDGGFADLHHP"
BASE COUNT          283 a          547 c          532 g          297 t
ORIGIN      
        1 ttcctggcgc acgaacccgg cgtgccggcc gccgctgttc agcaccaggt ccacgacggc
       61 ggtgatgccg gcagcggtcg tcacctgaat ggccgaccac aaccgtcccg cgatcatctg
      121 cgggtacacc ttgttcacgt agttctcttc acgcaggtcg ccgtcctgct tgcccgtcac
      181 agcaacgtag atcacgacga cgtcctgcag ggtctgcggc acggcgttct caagcacacg
      241 cttgagcgtg ccgcgatcgt gattgagctt cagatcgttc atcagcagcc gcatctgctc
      301 gcagtggccg ggatagcgca tggtcttgta gtccatgacc tgcgtgcgcg cgccgtaggt
      361 ctcggcgagg gagccgagac cgcccgaggt gttgaacgcc tcgtacaacg tgccgtcgat
      421 ctcgatctct tccagccctt cgagcggcgc cgcctcgatg cggcgaccgt caacgatcgc
      481 ttcgcacggg ttgccgtact cgttgatcag gccttcggtc gaccacgtca gcgaatactt
      541 caaaacgttg ttcgggtgct ggggcagggc gccgacgcgc agcttcactg tccgcaactc
      601 gtcgaagtgg ccgatgagct cgttcgcggc gatggagatg aagccgggcg cgagaccgca
      661 ctggggcacg aacgcttgct tggcgcccgc cgcgatcttg cgcactgcgc gcgtgacttc
      721 cacgtcttcc gtcaagtcga agtaactggt gccggcgcgg cgtgccgcct ctgctacggc
      781 gatattgcag tagtacggca gacccgagat taccgcgtcg accgggtggc tcttgagatg
      841 cgcatcgagc gccgcagtat cggtcgcgtc gagcttgtga gcgtgcaggt tgggaaagcg
      901 atgggcgttg acgactgcat tcgccgcctc gccgctgacg tcggcgagat gcacctcgta
      961 cgagccggac tcggcgagca ggccggagat cagcgctccg atcttgccgg cgccgagaat
     1021 gagaaccttg ggcatgcggg acctcccgga aaaaaggccg gcattctagc aattcccgca
     1081 gagtggtacg ctgcttccat gactcgcagc cccgatctca ttcagtggca ccctgcgagc
     1141 tggcagagca agccggcaca gcagcaggcg gcctacaccg atacagcagc gctgcaacgc
     1201 gctgttgcgt cccttgcgcg cctgccgccg ctggtcgttt cctgggaaat cgaggctctg
     1261 cgcgatcaca tcgcggcggc ccagcggggc gagcgcttca tactccaggg cggcgattgc
     1321 gccgagacct tcgaggactg cgagtccgac aagatcgcca agaagctgaa gatcctgctg
     1381 cagatgagcc tcgtgctcgt ctacggcctg cgcaagccgg tcgtgcgcat cggccgcatg
     1441 gccggtcagt acgccaagcc gcgctcggcg gacaccgaga cgcgcgatgg gcagacgctg
     1501 ccgagctatc gcggcgacat catcaatcat ccgggcttca cgccggacga acgcgatccg
     1561 gacccggaac tgctgttgcg cggctacgag cgagctgcgc tgacgctgaa cttcgtgcgc
     1621 gccctgatcg acggcggctt cgccgatctg catcacccc
//
LOCUS       DATANS010000267         1818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_560377, whole genome shotgun sequence.
ACCESSION   DATANS010000267 DATANS010000000
VERSION     DATANS010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560377"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..>1818
                     /locus_tag="VHT22_03830"
     CDS             59..>1818
                     /locus_tag="VHT22_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3136714.1"
                     /translation="MSGVVVERGCVAGVRYRGEHGEGEIRCESLVNCAGQWAREFGKL
                     ADVNVPLFSAEHFYIVTRPIAGVHGDLPVMRDPDGFIYYKEEVGGLVMGGFEPVAKPW
                     DVPAIPDRFEFQLLGEDWDQFEILMRNAIHRTPCLETAQVKMLLNGPESFTLDGNFIL
                     GEAPELRRYFVCAGFNSAGIANAGGAGRLMAEWIIGGDAPVDLWDVDVRRFAPFHANR
                     RHLFDRTAETLGLHYAMRWPREELATVRPLRRSPLYDRLKAKGAVFGSKLNWERANYF
                     LPSSSDVIPYTMDTPAWLPLVLDEQRAAREDVVVFDQTSFAKFILKGRDAVRVLQRLC
                     ANEVDVPPGRMVYTAMLNERGGFESDLTLTRIADDSFFILTGSAQATRDADWIVKHIG
                     DGEFAALVDVTSAYSVLSVMGPKAAELLGRLSPDDLSRQGLPFSETREIDVGYARVRA
                     ARISYIGGPGYELYVTADQCVTLYDALHEAGRALALRDAGYYTIDALRIEAGRRAWGA
                     ELGPDETPLEAGLAYAVRIDKASNFIGRDALLRQQAEGLKKRLLLFTFDDPAAFPWGG
                     EPISMNGQSVGELTSAGYSR"
BASE COUNT          311 a          564 c          611 g          332 t
ORIGIN      
        1 gcagtcgctg gcgaaagggg cccgcatgcg cggcgcgcgg atcttcgaag gcgcgaccgt
       61 gagcggcgtc gtcgtcgaac gcggatgtgt cgctggcgtg cgctatcgcg gcgagcacgg
      121 cgaaggcgag attcgctgcg agagtctggt caattgcgca ggacagtggg cgcgtgaatt
      181 cggcaagctc gccgacgtca atgtgccgtt gttctcggcc gagcatttct acatcgtcac
      241 ccggccgatc gccggcgtcc acggcgacct gccagtgatg cgcgatcctg acggattcat
      301 ctattacaag gaagaggtcg gcggcctggt gatgggtggc ttcgagccgg tagccaagcc
      361 gtgggacgtg ccggcgattc cggaccggtt cgagttccag ctgctgggcg aggattggga
      421 tcagttcgag atcctgatgc gcaatgcgat acatcgcacg ccctgcctgg aaaccgcgca
      481 agtaaaaatg ctgctgaacg gaccggagag cttcacgctg gacggcaatt tcattctggg
      541 cgaggcgccc gagctcaggc gttacttcgt ctgcgctggc ttcaactccg ccggcatcgc
      601 caacgccggc ggtgccggcc ggttgatggc cgaatggatc atcggcggcg atgcgccggt
      661 cgacctgtgg gacgtcgacg tccggcgctt cgcgccgttt catgccaatc gccggcatct
      721 gttcgatcgc accgccgaga cgctcgggct gcactatgcg atgcgctggc cgcgtgaaga
      781 gctggcgacc gtccggccgc tgcgccgctc acctctgtac gaccggctga aggccaaggg
      841 cgccgtattc ggcagcaagc tcaactggga gcgtgccaac tatttcctgc cgtcgagctc
      901 cgacgtcatc ccctacacca tggacacgcc cgcatggctg ccgctggtgc tggacgaaca
      961 gcgcgccgca cgcgaggacg tcgtcgtgtt cgatcagacc tcgtttgcca agtttatcct
     1021 caaaggccgc gacgccgtcc gggtgctgca acggctttgc gccaacgagg tcgacgtgcc
     1081 gccaggccgg atggtgtaca cggcgatgct gaacgagcgt ggtgggttcg aaagtgacct
     1141 gaccttgacg cggatcgcgg acgacagctt tttcatcctt accggctccg cgcaggcaac
     1201 gcgcgacgcc gactggatag tcaagcacat cggtgacggc gaattcgcgg cgctggtcga
     1261 tgtgacttcc gcctactcgg tgctctcggt gatgggcccg aaagcggccg agctgcttgg
     1321 ccggctttca ccggacgatc tttcacggca agggctgcct ttctccgaga cacgcgagat
     1381 cgatgtcggc tacgcgcgcg tacgcgccgc gcgcatcagt tacatcggcg ggcccggcta
     1441 cgagctttat gtgacggccg accagtgcgt gacgctgtac gatgcgttgc acgaggccgg
     1501 acgcgctctg gctttgcgcg atgccggcta ttacacgatc gatgctttgc gcatcgaagc
     1561 cggacggcgg gcctggggcg ccgaactcgg ccccgacgag acgccgctcg aagccgggct
     1621 cgcgtacgcg gtcagaatcg acaaagcgtc gaatttcatc ggccgcgatg cgctgctgcg
     1681 acagcaggcg gaaggcctga agaaaaggct gctgctgttc accttcgatg atccggccgc
     1741 cttcccgtgg ggcggcgaac cgatttcgat gaatgggcaa agcgtcggtg agttgacctc
     1801 cgccggctac agccgaaa
//
LOCUS       DATANS010000268         2048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_97964, whole genome shotgun sequence.
ACCESSION   DATANS010000268 DATANS010000000
VERSION     DATANS010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97964"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VHT22_03835"
     CDS             complement(<1..416)
                     /locus_tag="VHT22_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEX3136715.1"
                     /translation="MPPESPVKTAAPPLINRELSLLAFNRRVLALAQDASVPLLERLR
                     FLCIVSSNLDEFFEIRVAGMREQLRVKAPPPGVTLHELRTLSLRLCEQTHALVADIYR
                     TLNNEVLPAMAGAGIRLLRHAERNAVQRAWVADYFRR"
     gene            complement(502..1203)
                     /gene="dnaQ"
                     /locus_tag="VHT22_03840"
     CDS             complement(502..1203)
                     /gene="dnaQ"
                     /locus_tag="VHT22_03840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010102810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEX3136716.1"
                     /translation="MRQLILDTETTGLDPKDHRIIEIAAVELIDRRPTGRNVHFYINP
                     DQEIDTGATEVHGLSWEMLRDKPKFGEIADEFITFARGAQWVIHNAPFDVAFLDEEFT
                     RLGMSSSAAIAAEVVDTLALARDQFPGKRNNLDALCERFGVANAHRTLHGALLDAQLL
                     AEVYLAMTRGQESLTIDIVTPRLDVGEGLAAAGPVQLILVLPSPDELAAHRAYLVELD
                     RDSKGACLWLAVAGA"
     gene            complement(1215..>2048)
                     /locus_tag="VHT22_03845"
     CDS             complement(1215..>2048)
                     /locus_tag="VHT22_03845"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEX3136717.1"
                     /translation="LLLVVADGMGGHAGGEIAAQIAVRLFIERFQQEAKPTLKNPLKF
                     LQDSMLRAHQALGSYAEQFSMLETPRTTCVACIVQSNYAYWAHVGDSRLYLFRQGGLI
                     GQTKDHSKVQYLVDQGLIGAHEVVNHPDRNKIFSCLGGLVEPVIDLSRRTPLRNGDLI
                     VMCTDGLWSVYPQEEIARALTGAPILKSLPVLMREAEVRGGAEGDNLSLIAVRWGPET
                     LTDEPTTTITETMSLGEFQTEIDRTLTLTDRKGMQRDLTDDEIERAIAEIQATIKRYK
                     T"
BASE COUNT          345 a          651 c          686 g          366 t
ORIGIN      
        1 cgccggaagt aatcggcgac ccatgcgcgc tggacggcgt tgcgctccgc atgccgcagc
       61 aagcgtatgc cggccccggc catcgccggc agcacctcgt tattcagcgt gcgatagatg
      121 tcggcgacca gcgcatgcgt ctgttcgcac agtcgcaacg acagcgtgcg cagttcatgc
      181 agggtcaccc cgggcggagg cgccttgacg cggagctgct cgcgcatgcc ggccacgcgg
      241 atctcgaaga attcatccag attgctgctg acgatgcaca ggaagcgcag gcgttcgagc
      301 agcgggacgg aggcgtcctg cgccaacgcg aggactcgcc ggttgaacgc gagcagcgaa
      361 agctcgcggt tgatcagcgg cggcgcagcc gtcttgacgg gcgactcggg cggcatcggg
      421 gtatcaaagg cgccgtgcga cggtcatcgc ggcgaacccg gctagtgtcg ccggctcagg
      481 ttaccggaat gttacggcgg ctcaggcgcc ggcgaccgcc agccacaggc acgctccctt
      541 gctgtcgcga tcgagctcga ccaggtaggc gcgatgcgcg gcgagctcgt ccggcgaagg
      601 caaaacgagg atcagctgta ccggaccggc ggcggcaagg ccttcgccga cgtccaggcg
      661 cggcgtgacg atgtcgatcg tcagcgattc ctggccacgc gtcatcgcca gatagacttc
      721 ggcgagcagt tgcgcgtcga gcagggcgcc gtgcagggta cggtgggcgt tggcgacgcc
      781 gaagcgctcg catagtgcgt cgagattgtt gcgcttcccc ggaaactggt cgcgcgccag
      841 cgcgagcgtg tcgacgactt cggcggcaat ggcggcgctg gacgacatgc ccaggcgagt
      901 gaattcctcg tccaggaagg cgacgtcgaa cggggcattg tggatgaccc actgcgcgcc
      961 gcgtgcgaag gtgatgaact cgtcggcgat ctccccgaac ttcggcttgt cgcgcagcat
     1021 ttcccatgac aagccatgca cttcggtcgc gcctgtgtcg atctcctggt cggggttaat
     1081 gtagaagtgg acgttgcggc cggtcggccg gcgatcgatc agctcgaccg ctgcgatctc
     1141 gatgatgcga tggtccttgg ggtcgaggcc cgtggtttcg gtgtcgagga tcagttgccg
     1201 catggggaca gcatctaggt cttgtaccgc ttgatcgtag cctggatctc ggcgatcgcg
     1261 cgctcgatct catcgtcggt caggtcgcgc tgcatgccct tgcgatccgt cagcgtcagc
     1321 gtacggtcga tttcggtctg gaattcgccg aggctcatcg tctcggtgat cgtcgtcgtc
     1381 ggctcgtcgg tcagcgtttc cgggccccag cgcacggcga tcagcgacag gttgtcgccc
     1441 tcggccccgc cgcgcacctc ggcctcccgc atcagcaccg gcaacgactt cagtatcggc
     1501 gcgccggtga gcgcgcgcgc aatctcttcc tggggataga cgctccacag gccgtcggtg
     1561 cacatcacga tcagatcgcc attgcgcagc ggcgtccttc gcgacagatc gatcaccggc
     1621 tcgaccagtc caccgaggca gctgaagatc ttgttgcggt cgggatgatt gacgacttcg
     1681 tgcgcgccga tcaggccctg atcgaccagg tactggactt tcgaatggtc cttggtctgg
     1741 ccaatcaatc ctccctgccg gaacaggtac agccgtgagt cgccgacatg cgcccagtag
     1801 gcgtaattcg actgcacgat gcaggcgacg caagtggtcc gcggcgtctc cagcatcgag
     1861 aactgttcgg cgtacgagcc cagcgcctgg tgggcgcgga gcatcgaatc ctgcaggaac
     1921 ttcagcggat tcttcagcgt cggcttggct tcctgctgga agcgctcgat gaacagccga
     1981 acagcaatct gcgcggcgat ctcgccgccg gcatgaccgc ccatcccgtc ggcgacgacc
     2041 aacagcag
//
LOCUS       DATANS010000269         1633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_98087, whole genome shotgun sequence.
ACCESSION   DATANS010000269 DATANS010000000
VERSION     DATANS010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98087"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VHT22_03850"
     CDS             <1..323
                     /locus_tag="VHT22_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX3136718.1"
                     /translation="PRHPYTQALLSAIPVPAPGARAQRMILEGDVPSPVDPPSGCRFR
                     TRCPYAQALCAAETPPLAAEGGHAVACHFWRDIAPPATSIVAGHPLAAEVRLTRLQQA
                     FRGA"
     gene            409..>1633
                     /locus_tag="VHT22_03855"
     CDS             409..>1633
                     /locus_tag="VHT22_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020090809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3136719.1"
                     /translation="MNVKSWTARVGLALLAAFAATGAAAQTLRFGLAEDPDVLDPTLA
                     RTFVGRIVFASLCDKLVDIDEKLNIVPQLATSYEWSADHKALTMKLRSGVTFHDGEKF
                     DAAAVKYNIERHKTMAGSNRRGELAPVVSVDVIDASTVRLNLSAPFAPLLAALADRAG
                     MMVSPKAAQAEGDKFGAKPVCSGPFRFVERVAQDRIVVERYPNYWNKGEIHFDRIVYT
                     PIPDATVRLANLRSGQLDFIERMQASDVPQIKTDSRFKVEKITEIGYQGLTINTGKSD
                     LAQKNPLGRDAKVREALELALDRDGIVQVGMDGEADIGNQWVAPGNANYAKNVPIPKR
                     DVARAKELLREAGVTNPSFTLVMPTTSDAQKIAQVVQAMAKEAGFDVKLQATEFATSL
                     DMADKGQFEAYILAWS"
BASE COUNT          306 a          538 c          541 g          248 t
ORIGIN      
        1 ggccccgcca tccgtacacg caggcgctgc tttcggcgat cccggtgccc gcccccggtg
       61 cacgggcgca acggatgata ttggagggcg acgtgcccag tccggtcgac cccccgtcgg
      121 gctgccgctt tcgcacccgc tgcccctacg cgcaagcgct ctgcgcagcc gaaacgccgc
      181 cgctcgccgc ggaaggcggg catgcggtcg cctgccattt ctggcgcgac atcgccccgc
      241 ccgccacctc gatcgtcgcc ggccacccgc tggcggcaga ggtgcggctg acgaggctgc
      301 aacaggcctt ccgcggcgct tgagcattgg tcgaagcgag cgtattagcg ctaagatcgg
      361 gcgcagggcc gatcggcaaa tctttggcaa cagcgacagg agcaaacgat gaatgtgaaa
      421 tcatggaccg cgcgggtggg cctcgcgctg ctggctgcct tcgcagcgac cggcgccgcg
      481 gcgcaaaccc tgcgcttcgg cctcgccgaa gatcccgacg tcctcgatcc gacgctggcc
      541 cgcacctttg tcggccggat cgtgttcgcg tcgctgtgcg acaagctggt cgacatcgac
      601 gaaaaactca atatcgtgcc gcaactcgca acctcctacg aatggtcggc ggatcacaag
      661 gcactgacca tgaagctgcg gagcggcgtg actttccacg acggcgagaa attcgacgcg
      721 gcggccgtca aatacaacat cgaacgccac aaaacgatgg ctggctcgaa ccggcgcggc
      781 gagctcgcgc cggtggtgag cgtcgatgtc atcgacgcat cgaccgtgcg attgaacttg
      841 tcggcgcctt tcgcgcccct gctcgccgcg ctggcggatc gcgccggcat gatggtctcg
      901 ccgaaggcgg cgcaggccga gggcgacaag ttcggcgcca agccggtctg ctcggggccg
      961 ttccgcttcg tcgagcgcgt cgcgcaggac cgcatcgtcg tcgagcgcta tccgaactac
     1021 tggaacaagg gcgagatcca cttcgaccgc atcgtctata cgccgatccc ggatgcgacg
     1081 gtgcggctgg ccaacctgcg ctcgggtcaa ctcgacttca tcgagcggat gcaggcttcc
     1141 gacgtgccgc agatcaagac cgatagccgc ttcaaggtcg agaagattac cgaaatcggc
     1201 taccagggcc tcacgatcaa cacgggcaag agcgatctgg cgcagaagaa tccgctgggc
     1261 cgcgacgcga aggtgcgcga agccttggag ctcgcgctgg accgcgacgg catcgtccag
     1321 gtcgggatgg acggcgaagc cgacatcggg aatcagtggg tcgccccggg caacgccaac
     1381 tacgcgaaaa atgtcccgat tccgaaacgg gatgtcgcgc gcgccaagga gctgctgcgc
     1441 gaggcgggag tgacgaatcc gagcttcacg ctggtgatgc cgacgacctc ggacgcgcag
     1501 aagatcgcgc aggtcgtgca ggcgatggcc aaggaggcgg gattcgatgt caagctgcag
     1561 gcgaccgagt tcgcaacctc gctcgacatg gccgacaagg ggcagttcga ggcctacatc
     1621 ctggcatgga gcg
//
LOCUS       DATANS010000270         6486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_435145, whole genome shotgun sequence.
ACCESSION   DATANS010000270 DATANS010000000
VERSION     DATANS010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435145"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1402)
                     /locus_tag="VHT22_03860"
     CDS             complement(<1..1402)
                     /locus_tag="VHT22_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136720.1"
                     /translation="MTPAQIDRVFGRERLKMVTGEHVEVYREAVAPGEPRRYTKRFLA
                     TTNGDFGPWTEREWRILARLIGHGIRCVPEVVQFDAGAKSGMRSVQTCDAGVTVDQWA
                     TLVPVARDGTVRGHIFEDSAHWWSLAHYCLAALSEIHALELVHLDIKADNICIPYAPA
                     DFEPDGDEGRLYPAFARLALIDFAFSLVSRESLALPLPLGWQKDYDYQSPRLLKALEA
                     GRNGDLEPTRVLDWRCDFYSLAAMLKRYLPSDRRGGDGSEMGWTSNRYDDARSLIFRL
                     RDTHDRDLPHWRPHAELIDFCAARLDEPDLVASLQKGWTLARGEIAAGMATPITPITP
                     VTRIAPFARAASVTPLTHIASSEPIVLPGLAEPSAARGEMAAIGPDLLRMKPRAVRSE
                     TAAGPHLLRRTEVQAPWRAKLLLLSLVAGVAASAPPFIGNPAHPLFERASPASGERNA
                     TSPVQAPRSSLSSNGAA"
     gene            complement(1399..2358)
                     /locus_tag="VHT22_03865"
     CDS             complement(1399..2358)
                     /locus_tag="VHT22_03865"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEX3136721.1"
                     /translation="MRTNVEAIRVPSPTPTPRTSACGAARVREAVTATRWARVHAAVA
                     SSRGRQHAVNEDQHSPLDGNAELFVVADGVGSGALASCASRELVAHIHAVLGHGRVGA
                     DAVRTAILEADREIARSIARRTSTPGAATVALCVAINARRSQWLVAWVGDCRAYRLRA
                     SREDGTELLTVDDTYRQLNERSPSGAGPDDPARMVGNGAVDAPNVRKVELSAGEMLVL
                     CSDGVHRHAAAGDIARVLRTSAPLGERCARLVEFARQRGSSDDATVLVVQPSTSISAR
                     IARGFSLALIVAFLAAAGAWLATHDSVSRLFAQANVNVPKVQP"
     gene            complement(2370..3710)
                     /locus_tag="VHT22_03870"
     CDS             complement(2370..3710)
                     /locus_tag="VHT22_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX3136722.1"
                     /translation="MREPSVGVFEHGPGTIDEAEHLGPYAPLIGSIRSELEQFAASQL
                     RLHLAIAERDRYLLTSIELECEGNDHERELLRRFVREFRPDQIKRYLAKEVIARLRNA
                     SAIDLSQFAGLNAEVDTADLAREREEERYGDLIAELSSAMPHSAGNPYRVTLVGRWSE
                     VDARAQSRDDKAPGPEALRTPLAARAIQVDIEDGAGTHHIELKSIAAGRRYSVGKGEG
                     CDIVVDGVYVSRRHCEIWLENGTWRVADTGSTNGIRIESTTGVIARLPSDGISARPAT
                     VELPLGAWLVLSARMRGEPAQYPRLSLHLGGDLEQSPAKGTPAKAVATPVTPIAPPRR
                     RDHAWTLTARMASGSRNIDIGNDALPLRIGRSRNQALVVDWDHSDVSGHHLDIVSVDE
                     SGATALVLGDNGVTVDGTFHGPGTEFRWQPGETLKLDQGTDRVAACELTLSRAG"
     gene            complement(3825..4067)
                     /locus_tag="VHT22_03875"
     CDS             complement(3825..4067)
                     /locus_tag="VHT22_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136723.1"
                     /translation="MEDTLRRLIVQLGEVAAISVLIELSLPALKRRVDAGDAVQPAWG
                     EFLDRLAQRYGLPAAARVRPLRDDGPLAILVIAYRS"
     gene            complement(4178..>6486)
                     /locus_tag="VHT22_03880"
     CDS             complement(4178..>6486)
                     /locus_tag="VHT22_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136724.1"
                     /translation="SSPASRIAAQLQTIDHALAAFSSIDNRRVSDVVGFDAGRWFDAV
                     IAALGTPIETPEYPDQRFALRCEDIAAAVAALARSDGRILAAFAWRGTEVGRVVAAWR
                     PEQFVAIAAHGLGRNNPWAGIPGCIYLGASAPAGSDAKTYFAAGARGIEDLVCARAEM
                     RGISAEDAQRSPALSIAGEPTLDMPVNDERWKIPPSLGAILQPLEALHRPAGALYRAY
                     TGAQSDAQAMRTAYRYGPNRIELYGAGVDVGYSIDLTIDPTLQEIAQRMAACYTGRTD
                     VCRAFGIVRKDDGARAVGDGLLEHALVRMAAVAVIDVETGRIEALAGALSPCTRQEYD
                     GPARSARCDQRLPYATRYRPAALLNPAVFHDAMPGSTIKPIMAAAFLTDGSDGARLLT
                     SERADMTRSPTAVPSAESLRGQLARSNSARFLDRMFCADLGFAPCARPREIQTMALAF
                     GWNGACAEPSDQCGKQDLLFGRPPDARVDDGGIRPLSLDIPYGRLLTQPVSTKVGAPF
                     KLRPDVALDRARLQQCAAGADGKRFNRDDWGKCRSSVVDIVAEGWGQGQARASALGVA
                     GMMAMLAAAANGQAEVRAPHLVDALRGVAAAGGQRAEAAAARVSLVEARQNRIPHEAA
                     EVIIDGLSFSHRAGTARLACEQVFDARTCARMDWIAGKTGTPTFPNDTRSLSELARLC
                     AGPVLRTRRDRDACGPLRPYKWYVAAYRTNPDDPRWTKVIGVLTERNWFVASGRVHGA
                     GDQGPNPAAEIGMQIAARHAGVIPGTSE"
BASE COUNT         1046 a         2211 c         2200 g         1029 t
ORIGIN      
        1 cggcggcgcc gtttgaactt aagctcgaac gcggagcctg gaccggtgag gtcgcattcc
       61 gctcgcccga tgcagggctc gcacgctcaa acagaggatg tgcgggattg ccgatgaacg
      121 gcggcgccga cgcggcgacg cccgcgacca gcgaaagcaa gagcagcttc gctcgccagg
      181 gcgcttgcac ttccgtccgt cgaagaaggt gcggtcccgc ggccgtttcg ctgcgaacag
      241 cgcggggctt catacgaaga aggtcggggc caatcgccgc catttcgccg cgggcagcgc
      301 tcggctccgc aagcccggga agcacgatcg gctccgagct cgcgatgtgg gtgagcgggg
      361 tcaccgaagc ggcgcgcgcg aaaggcgcga tgcgcgtgac aggtgtaatg ggcgtgatcg
      421 gcgtcgccat gcccgccgcg atctcgccgc gcgcgagcgt ccaacctttc tgcagcgagg
      481 cgacgagatc gggctcgtcc agccgcgccg cgcagaaatc gatcagctcc gcgtgcggac
      541 gccagtgcgg caggtctcga tcatgggtat cgcgcagccg gaagatcagc gaccgcgcat
      601 cgtcatagcg attcgatgtc cagcccatct ccgagccgtc accgcctcga cgatcggacg
      661 gcagatagcg cttgagcatc gccgcgaggc tgtagaagtc gcagcgccag tcgagcaccc
      721 tggtcggctc gaggtcaccg ttgcggccgg cttcgagcgc cttgagaagg cgcggcgatt
      781 gatagtcgta atccttctgc cagccaagcg gcagcggaag cgcgagactc tcgcgactca
      841 cgagcgaaaa cgcgaagtcg attagcgcga gccgcgcaaa ggccggatac agacgacctt
      901 cgtcgccatc gggctcgaag tcggccggcg cgtacggaat gcagatattg tcggccttga
      961 tatccaggtg aacgagctcg agcgcgtgaa tctcgctcag cgcggcaaga cagtaatgcg
     1021 cgagcgacca ccagtgtgcg gaatcctcga agatatgccc gcgcacggtc ccgtctcgcg
     1081 cgacgggaac aagtgttgcc cattggtcga cggtaacgcc cgcgtcgcac gtttgcacgc
     1141 tgcgcatgcc gctctttgca cccgcgtcga actgcacgac ttcgggcacg cagcggatgc
     1201 cgtgaccgat cagacgcgcg aggatgcgcc actcgcgttc cgtccacggc ccgaagtcgc
     1261 cgttcgtcgt cgcgaggaag cgcttggtgt agcgccgagg ctcgccggga gcgactgcct
     1321 cgcgatacac ctcgacgtgc tcgccggtaa ccatcttgag gcgttcgcga ccgaatacac
     1381 ggtcgatttg tgctggtgtc atggctgcac ctttgggaca ttgacgttcg cctgcgcgaa
     1441 gaggcgcgac accgagtcat gcgtggcgag ccacgcaccc gctgccgcga gaaacgcgac
     1501 gatgagggcc aacgagaatc cgcgcgcgat cctcgcactg atgctggtcg acggctgcac
     1561 gacgagcacc gtggcgtcgt cgctgctccc tcgctgccga gcgaattcga cgagccgcgc
     1621 acagcgctcg ccgagtggcg cagaggtgcg aagaacacgc gcgatatcgc ctgctgctgc
     1681 gtgccgatgg acgccgtcgc tgcacagaac cagcatctcg cccgccgaga gctcgacctt
     1741 ccggacattc ggggcatcga cggcgccgtt gccgaccatc cgcgccggat cgtccgggcc
     1801 tgcgcccgaa ggcgagcgct cattgagctg ccgataggtg tcgtcgacgg tgagaagctc
     1861 ggtcccgtct tcgcgcgacg cacgcagtcg atacgcgcgg cagtcgccga cccacgcgac
     1921 cagccactgc gagcgccgtg cattgatcgc cacgcacagc gcgaccgtcg ctgcgccggg
     1981 cgtgctcgtg cgccgcgcga tgctccgcgc gatctcgcgg tccgcttcga ggatcgctgt
     2041 cctgaccgcg tcggcaccga cgcgtccgtg accgagcacc gcgtgaatgt gcgccacgag
     2101 ctcgcggctc gcgcacgacg ccaacgcgcc cgacccgacg ccgtccgcga cgacgaagag
     2161 ttcagcgttt ccgtcgagcg gactgtgttg gtcttcgttc acggcatgct gccgcccgcg
     2221 cgacgacgcg acggccgcat gaacgcgggc ccatcgcgtt gccgtcaccg cttcgcgcac
     2281 gcgcgcggcg ccgcaggcgc tcgtgcgcgg ggtaggcgta ggcgaaggca cccggattgc
     2341 ttcgacgttt gtcctcattc gacgaggcct cagccggcgc gtgacagcgt gagctcgcac
     2401 gccgcgacgc ggtcggtccc ttgatcgagc ttgagcgtct cgcccggttg ccagcggaac
     2461 tcggtgccgg gcccgtgaaa tgtgccatcg acggtgacgc cgttgtcgcc aagcacgagc
     2521 gccgtggcgc cgctttcgtc gaccgagacg atgtcgagat ggtgtcccga gacatcgctg
     2581 tgatcccagt cgacgacgag cgcctggttg cgcgagcggc cgatgcgcag cggcagcgca
     2641 tcgttgccga tgtcgatgtt gcgcgagccc gacgccattc gagcggtgag cgtccacgcg
     2701 tgatcgcgcc gtcgcggtgg agcgatcggc gtcactggcg tcgcgacggc tttcgcgggc
     2761 gttcccttgg ccggtgactg ctcgaggtcc ccacccagat gcagtgacaa gcgcggatat
     2821 tgagccggct caccgcgcat gcgcgcggaa aggacgagcc acgcgccgag aggcagctcg
     2881 accgtcgccg gacgtgcgga gatcccgtcc gaaggcaagc gcgcgatcac gccggtcgtc
     2941 gactcgatgc gaatcccgtt cgtcgagccg gtatcggcca cgcgccatgt cccgttctcg
     3001 agccagattt cgcaatgccg tcggcttaca taaacgccgt cgaccacgat gtcgcagccc
     3061 tcgcctttgc ccacggagta ccgccggccc gccgcgatcg acttgagctc gatgtggtgc
     3121 gtgccagccc cgtcctcgat gtcgacctgg atcgcgcgcg cggcgagcgg tgttcgcagc
     3181 gcctcgggcc ccggcgcttt gtcgtcgcgc gattgcgcgc gcgcatcgac ctccgaccag
     3241 cgccccacga gcgtcacgcg atacgggtta cccgcgctgt gcggcatggc ggacgaaagc
     3301 tcggcaatga gatcgccgta gcgctcctcc tcgcgctccc gggcgaggtc agcggtatcg
     3361 acttcggcgt tcaggcccgc gaattgcgac aggtcgatcg cgcttgcgtt gcgcagtcgc
     3421 gcgatcactt ccttggcgag ataacgcttg atctgatccg gccggaactc gcggacgaag
     3481 cggcgcaaga gctcccgttc gtggtcattg ccttcgcact cgagctcgat cgaggtaaga
     3541 aggtagcggt cacgctcggc gatcgcgaga tgcagccgaa gctggctcgc tgcgaactgc
     3601 tcgagctcgc tgcgtatcga gccgatcagc ggcgcgtagg ggcccaggtg ctcggcttca
     3661 tcgattgtgc cgggcccatg ctcgaacaca ccgacactcg gctcgcgcat ccgcgccgcg
     3721 cccggagcgt cgccacgcgc tgcatcacgc gcttgcgacg gcggatcttg tcgccctccg
     3781 gcgttcatct ttcccagcat ccagcgcttc acggccatga tgtcctagct cctgtaagca
     3841 atgacgagga tcgccagcgg cccgtcgtcg cgaagcgggc gcacgcgtgc agcggcaggc
     3901 agtccatacc gttgtgcgag gcggtcgagg aattcgcccc aggcgggttg gaccgcgtcg
     3961 cccgcatcga cgcgccgctt gagcgcaggc agcgacagct cgatcagtac gctgatcgcc
     4021 gcgacttcgc ccagctgcac gatcagccgt cgcagcgtgt cctccatatc ggcgatgcag
     4081 gtcgctaacg gaccgtcgaa cgtccatacg aaaggtccgc cgatcgcgct cgaggcgcgt
     4141 ccgatcgacc gccgccgaat tctgtgcgac gcgggcttca ctcggacgtt cccggtatca
     4201 cacccgcgtg gcgtgcggcg atctgcatgc cgatctcggc cgcaggattc ggaccctggt
     4261 cgccggcacc gtgaacgcgg cccgacgcga cgaaccagtt gcgttcggtc agcacgccga
     4321 tcaccttcgt ccaccgcggg tcgtcgggat tcgtccgata tgccgcgacg taccacttgt
     4381 agggccgcag tggcccgcag gcatcgcggt cgcgccgagt cctcaagacg ggcccggcgc
     4441 acaaccgggc gagctcggac agcgagcgcg tatcgttggg aaacgtgggc gtgccggttt
     4501 tgccggcgat ccaatccatg cgcgcgcagg tgcgcgcatc gaagacctgt tcgcaggcga
     4561 ggcgggcagt gcccgcgcgg tggctgaagg acaggccgtc gatgatgacc tcggcggctt
     4621 catgcggaat ccgattttgc ctcgcttcga cgaggctcac gcgcgcggcg gccgcctccg
     4681 ctcgctggcc gccggcagcc gccacgccgc gcagcgcgtc gaccagatgt ggcgcccgca
     4741 cctcggcctg gccgttggcg gctgctgcga gcatggccat catgcccgcg acgccgagcg
     4801 cgctcgcgcg cgcctggccc tggccccacc cttccgcgac gatgtcgacc acgctgctgc
     4861 ggcatttccc ccagtcatcg cgattgaacc gcttgccatc ggcaccggcg gcacattgct
     4921 gcaaccgggc gcgatccagc gcaacgtcgg ggcgcaactt gaaaggcgcc ccgaccttcg
     4981 tgctcacggg ctgcgtcagc agccggccat acgggatgtc gagcgagagc gggcggatgc
     5041 cgccgtcgtc cacgcgcgcg tcgggagggc gcccgaacag aaggtcctgt ttgccgcatt
     5101 ggtcgctcgg ctccgcacac gcgccgttcc atccgaaagc gagcgccatc gtctggatct
     5161 cccgcggacg cgcgcacggc gcgaacccga gatccgcgca gaacatgcga tcgagaaagc
     5221 gtgcggagtt ggagcgcgcg agttgtcctc gcaggctctc ggccgatgga acggccgtgg
     5281 gagatcgcgt catgtcggcg cgctcgctgg tgagcaggcg cgcgccgtcc gagccgtccg
     5341 tgagaaacgc tgccgccatg atcggcttga tcgtcgagcc cggcatcgca tcgtgaaaga
     5401 cggccggatt cagcaatgca gccggacgat agcgcgtggc atacggcaaa cgctgatcgc
     5461 aacgcgccga gcgagcgggc ccgtcgtact cctgtcgtgt gcagggggaa agcgcacccg
     5521 cgagcgcttc gatacggccg gtttcgacgt cgatgacggc gaccgccgcc atgcgcacca
     5581 gcgcgtgctc gagcagcccg tcgccgaccg cgcgcgcgcc gtcgtccttg cgcacgatgc
     5641 cgaaggcgcg acaaacatcg gtgcggccgg tgtagcacgc ggccatcctt tgggcgatct
     5701 cctgcagcgt cggatcgatc gtgagatcga tggagtaccc gacgtcgact ccggcgccgt
     5761 agagctcgat gcggttcggc ccgtaacgat acgcggtgcg catggcctgc gcgtcgcttt
     5821 gcgccccggt atacgcgcga tacagcgcgc cggccggccg atgcaatgcc tcgagcggct
     5881 gcagtatcgc tccgagcgaa ggcgggatct tccagcgctc atcgttgacc ggcatgtcga
     5941 gagtcggctc tccggcgatt gaaagggccg gcgaacgctg cgcatcctca gcggagattc
     6001 cacgcatttc ggcgcgcgcg catacgaggt cttcgatacc gcgcgctccg gcggcgaagt
     6061 acgtcttggc atcgctccct gccggcgccg atgcaccgag atagatgcag cccggaatcc
     6121 cggcccatgg attgttgcgc ccgaggccgt gcgcagcgat cgcgacgaac tgttcgggac
     6181 gccaggcggc gacgacgcgc cctacttccg tgccccgcca tgcgaaggcg gcgaggatgc
     6241 gcccgtcgga acgcgcgaga gcggcaacgg cagcggcgat gtcctcgcac cgcagcgcga
     6301 agcgctggtc gggatactcg ggcgtttcga tcggtgtgcc cagcgcggcg atgacggcgt
     6361 cgaaccagcg tccggcatcg aagccgacga cgtcgctgac gcggcgattg tcgatgctgc
     6421 tgaaagcggc gagcgcgtgg tcgatcgttt gcagctgtgc agcgattcgg gacgcaggcg
     6481 atgaat
//
LOCUS       DATANS010000271         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_560601, whole genome shotgun sequence.
ACCESSION   DATANS010000271 DATANS010000000
VERSION     DATANS010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560601"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1541)
                     /locus_tag="VHT22_03885"
     CDS             complement(<1..>1541)
                     /locus_tag="VHT22_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX3136725.1"
                     /translation="LGAEPRRRDLLIEHLHKIQDRYGCISAAHLVALSREMALAYAEV
                     YEVATFYHHFDVIKEGDAPPPKLTVRVCDSLSCELAGALPLLEKLRATLGANVRVIPA
                     PCIGRCEQAPAAVVGQNPVACATAASVEAAVKAGTTRCAVAKYIGYDEYRKKGGYRIA
                     AECLAGKRDAEDILKTMEASSLRGLGGAGFPTGRKWRILRRERGPRLMAINIDEGEPG
                     TFKDRYYLERDPHHFLEGAVIAAWTAQISEIYIYLRDEYAGCRAILEREIAALQKNPP
                     CPLPPIHLRRGAGAYICGEESAMIESIEGKRGMPRLRPPYVAQVGLFGQPTLEHNMET
                     LQWVRDILEKGAEWFAGHGRHGRKGLRSFSVSGRVNKPGVHLAPAGITVNELIAEYCG
                     DMLPGHKFYAYLPGGASGGILPASMGDIPLDFDTLQPYGCFIGSAAVIILSDRDNASA
                     AARNLMKFFADESCGQCTPCRVGTAKALHLMQAPRWNKALLEELSVAMADASICGLGQ
                     AAPNP"
BASE COUNT          256 a          507 c          501 g          277 t
ORIGIN      
        1 cggattgggc gccgcctgac caagcccgca tatggatgcg tctgccatcg cgacggaaag
       61 ttcctcgagc aacgccttat tccagcgcgg cgcctgcatg aggtgcagcg ccttggcggt
      121 gccgacgcgg cacggcgtgc attgcccgca tgactcgtcg gcgaaaaact tcatcaggtt
      181 gcgcgcggcg gcgctggcgt tgtcgcggtc cgagaggatg atgaccgcgg cggagccgat
      241 aaagcaaccg tacggctgca gcgtatcgaa gtcgagcggg atgtcgccca tgctcgcggg
      301 cagtatgccg cccgacgcgc cgccgggcag gtacgcgtaa aacttgtgtc cgggcagcat
      361 gtcgccgcag tactcggcga tcagttcgtt cacagtgatg ccggcgggcg cgagatgcac
      421 gccgggttta ttgacccgcc cggataccga gaacgagcgc aggcccttgc gcccgtgacg
      481 gccgtggccg gcgaaccact cggcgccttt ctcgagaata tcgcgcaccc actgcagcgt
      541 ttccatgttg tgctcgagtg tcggctgccc gaacaggccg acctgcgcga catacggagg
      601 gcgcagccgt ggcatgcctc gcttgccttc gatcgattcg atcatcgccg actcttcgcc
      661 gcagatgtac gcccccgcgc cgcgccgcaa atggatcggc gggagcggac acggcggatt
      721 tttttgcagc gccgcgattt cgcgctccag aatcgcgcgg caacctgcat attcgtcgcg
      781 caagtagata tagatttcgg aaatctgcgc cgtccacgcc gcgatcaccg cgccctcgag
      841 aaagtggtgc gggtcgcgct cgaggtaata gcggtccttg aacgtgcccg gttcgccctc
      901 gtcgatgttg atcgccatca agcgcgggcc gcgctcgcgg cgcagaatgc gccatttgcg
      961 gccggtcgga aatcccgcgc cgccaagccc gcgcaggctc gatgcctcca tggttttcag
     1021 aatgtcttcc gcatcgcgct tgccggcgag acattcggcc gcgatcctgt aaccgccttt
     1081 tttccggtac tcgtcgtagc caatatactt cgcgaccgcg catcgcgtag tgccggcttt
     1141 caccgcggct tcgacgctgg ccgcggtcgc acaggccacc ggattctgcc cgacgaccgc
     1201 cgcgggcgcc tgctcgcaac ggccgatgca gggcgccgga atcacgcgca cgttggcgcc
     1261 gagcgttgcg cgcagcttct ccagcagcgg cagtgcgccg gccagttcgc acgacagcga
     1321 atcgcatacg cgcaccgtca gtttgggtgg cggcgcgtcg ccctctttga tgacgtcgaa
     1381 atgatggtag aaggtcgcga cttcgtagac ctcggcgtac gcgagtgcca tttcacgcga
     1441 aagcgccacg aggtgggcgg ccgaaatgca gccatagcgg tcctggattt tatgcagatg
     1501 ctcgatcagg aggtcgcgcc ggcgcggctc cgcgccgagc a
//
LOCUS       DATANS010000272         2702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_13438, whole genome shotgun sequence.
ACCESSION   DATANS010000272 DATANS010000000
VERSION     DATANS010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13438"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1615
                     /locus_tag="VHT22_03890"
     CDS             74..1615
                     /locus_tag="VHT22_03890"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015888233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX3136726.1"
                     /translation="MSLFVMQGVSKRYGGVRALQDADLTIEAGRIHAVLGENGAGKST
                     LIKIMAGVVAPDEGRMLLDGDPVSFASPAAANEAGIACIFQELSLIPDLSVADNIAIS
                     NPPRRFGLIDRRAQRRMAEGALARAGADNIHPLALVKDLPLSRRQMVEIAKALARKPR
                     ILILDEATSALTAADVTKVYSVLKRLRAEGLALVYISHRMHEIAELADECTVFCNGRS
                     VSSFAAGTKTDNEIVELMIGREYNAAFPARATRAPGDAPPLLEVRHLCWTDRLTDVSL
                     RLSAGEVIGLGGLDGQGQRELLLALFGVLLGATGEVLIDGRAVAITGPHVAKGKQIGM
                     ALIPEDRKTEGLMLPMSVRDNLSFAALDTLSRWGVVDRAAERDAVERIVRLLGIRTDG
                     TDLPAGSLSGGNQQKLVIGKWLMMAPRILLLNDPTRGIDVGTKQEMYQLLHQLAVAGA
                     AILFYSTDYDELVGCCDRVLVMYDGAIKRELIGAEVTERALISSALNIGVEGRKSGPP
                     QATLS"
     gene            1612..2595
                     /locus_tag="VHT22_03895"
     CDS             1612..2595
                     /locus_tag="VHT22_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3136727.1"
                     /translation="MSGDWSYWLREHKGTLLAFAGFVVMFTIYAANHPMGLNANVATT
                     AANKGVLLAIVAMAQTLVVLTSGIDLSVGMVMVLANCLASSIVIGSPLATALGVAGVL
                     VTGCVCGAINGLIVIYGRLQPIVTTIATGAVYYGIALALRPVPGGDVNASLADALTGQ
                     LPGGVPASLAVLLALVAIVWLPFRRSAIGRAAYALGSSEIAAYMSGVPIRRAKFVAYT
                     LSGLLASVGGLFLTFITYTGEASAANGGAYTLYSIAAVVLGGVSLFGGSGSAVGAIFG
                     ALMFRTIGDLLFVFNVEPLWQPLFQGMVLLAAVCLGSVRLLRVRNRLDLYG"
BASE COUNT          424 a          861 c          915 g          502 t
ORIGIN      
        1 gcaggaaatc atggggcaga gcgaagcgaa caagtaagcg cgggtgcggg gacttcggtc
       61 cccgcgctta ccgatgagcc tgttcgtcat gcaaggcgtc tccaagcgct acggcggcgt
      121 gcgcgcgctt caggacgccg acctgaccat cgaagccgga cgcattcatg cagtgctcgg
      181 tgagaacggg gcaggaaagt cgacgctgat caaaatcatg gccggcgtcg tcgcacccga
      241 cgagggccgc atgctgctcg acggcgaccc ggtttcattc gcatcgccgg ccgcggcgaa
      301 cgaagcgggc atcgcctgta tcttccagga attgtcgctg atccccgatc tttcggttgc
      361 cgacaacatc gccatcagca atccaccgcg ccgcttcggt ctgatcgacc gccgggcgca
      421 acgccgcatg gccgaggggg cgctggcgcg tgccggcgcc gacaacatcc atccgctggc
      481 gctggtcaag gacctgccgc tgtcgcggcg gcagatggtg gagatcgcca aggcgctcgc
      541 ccgcaagccg cgcatcctga ttctcgacga agccacctcg gcgctgaccg ccgccgacgt
      601 gaccaaggtc tacagcgtcc tgaagcgact gcgcgcagaa ggactcgcgc tggtctacat
      661 ttcgcaccgc atgcacgaga tcgccgaact ggccgacgag tgcacggtgt tctgcaacgg
      721 acgcagcgtc tcgagcttcg ccgcgggcac caagaccgac aacgagatcg tcgagctgat
      781 gatcggccgc gaatacaacg ccgcgtttcc ggcaagggct acgcgcgcgc ccggtgatgc
      841 gccgccgctg ctcgaggtgc gacacctgtg ctggaccgac cgcctgaccg acgtctcgct
      901 ccgactgtcc gccggggagg tgatcggtct gggcggactc gatggccagg gtcagcgaga
      961 gctgttgctg gcgctgttcg gcgtgttgct cggcgcgacc ggcgaggttc tgatcgacgg
     1021 ccgggccgtg gcgatcaccg ggcctcatgt ggccaagggc aagcagatcg gcatggcgct
     1081 gatcccggag gaccgcaaga ccgagggcct gatgttgccg atgtcggtgc gcgataacct
     1141 gtcgttcgcc gcgctcgata ccttgtcgcg ctggggcgtg gtcgacaggg cggccgaacg
     1201 cgatgcggtg gagcgtatcg tgcggctgct cggcatacgc accgacggca ccgaccttcc
     1261 ggccgggtcg ctgtctggcg gcaatcagca gaagctggtc atcggcaagt ggttgatgat
     1321 ggcgccgcgc atcctcctgc tgaacgatcc aacgcgcggt atcgacgtcg gcaccaagca
     1381 ggagatgtac cagctgttgc accagcttgc cgtcgcggga gcggcaatcc tcttctattc
     1441 gaccgactac gacgagctcg tcggctgttg cgatcgcgtc cttgtcatgt acgacggcgc
     1501 gatcaagcgg gaattgatcg gcgccgaagt caccgaacgc gcgttgataa gcagcgcctt
     1561 gaacattggc gtcgaaggcc gcaaatccgg cccgccgcag gcgacgctgt cgtgagcggc
     1621 gactggtcgt actggctgcg cgagcacaag ggcacgctgc tcgctttcgc gggttttgtg
     1681 gtcatgttca cgatctacgc tgccaaccat ccgatgggac tcaatgccaa tgtcgcgacc
     1741 acggcggcca acaagggcgt gctgctcgcg atcgtcgcga tggcacaaac actggtcgta
     1801 ctgacgtcag gcatcgatct gtcggtgggg atggtgatgg tgctcgccaa ttgcctggcc
     1861 tcgagcatcg tcatcggctc accgcttgcg acagcgctcg gtgtcgccgg cgtgctggtc
     1921 accggttgcg tgtgcggcgc gatcaacgga ctgatcgtca tctacggacg tctgcagccg
     1981 atcgtcacca ccatcgccac cggcgcggtg tactacggaa tcgcgttggc gctgagaccg
     2041 gtccccggtg gcgacgtcaa cgcctcgctg gccgatgcgc tgaccggcca gctgcccggc
     2101 ggcgtgccgg caagtctcgc ggtgctactg gcgctggtag cgatcgtatg gctgccattc
     2161 cggcggtcgg cgatcggccg tgccgcttat gcattgggat cgtccgagat cgcggcatac
     2221 atgtcgggcg tgccgatccg ccgggccaag ttcgtcgcgt acacgctgtc gggtctgctc
     2281 gcgtcggtag gtgggctgtt cctcaccttc atcacctata ccggcgaggc gtcggctgcc
     2341 aacggtggcg cttatacgct gtattcgatc gctgcggtgg tgctgggcgg ggtgtcgctg
     2401 ttcggaggat cgggcagcgc ggtcggcgcg atcttcggcg cgctgatgtt ccgcaccatc
     2461 ggcgatcttc tgttcgtgtt caacgtcgag cctttgtggc agccgctgtt ccagggcatg
     2521 gtgctgctcg cggcggtctg cctgggctcg gtccggttgc tgcgcgtacg caaccggctc
     2581 gacttgtatg gctgaggctc ccgctcccgc caaccggctg ggcagcatgc ggcggcgcat
     2641 cgacccagcg atcgcgtggg ccttcggctg catcatcgtc gtcctgttta ttggcagcct
     2701 ct
//
LOCUS       DATANS010000273         1951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_13443, whole genome shotgun sequence.
ACCESSION   DATANS010000273 DATANS010000000
VERSION     DATANS010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13443"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..449)
                     /locus_tag="VHT22_03900"
     CDS             complement(36..449)
                     /locus_tag="VHT22_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX3136728.1"
                     /translation="MTSLIRYFDPLDRVFDRAFGAVLANGDANATSRSIALDVVETPE
                     AYIVKAELPGIPKDKIEVNVEDRNVTIGASFSQEIDANGKTLWQERNFGKLSRAIRLP
                     EPVDANGTQATHVDGVLQLTLPKVAKTNSKQITIQ"
     gene            complement(535..1119)
                     /locus_tag="VHT22_03905"
     CDS             complement(535..1119)
                     /locus_tag="VHT22_03905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX3136729.1"
                     /translation="MLHILHQIARTGIKSETAPTPAEEMIVVARLQKTILRTLGRALT
                     IRHVDAGSCNGCELEIQALTNPYYNLEGIGIRFVASPRHADMLLVTGPVSKHMAIALK
                     RTYDATPEPKLVVAVGDCGCTGGIFGQSYASLGGVASVIPVDVAIPGCPPSPRALLQG
                     ILAAISAHAPPVAAQADRPAVEPTLRSPDAPDTP"
     gene            complement(1122..>1951)
                     /locus_tag="VHT22_03910"
     CDS             complement(1122..>1951)
                     /locus_tag="VHT22_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007735010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ni,Fe-hydrogenase III large subunit"
                     /protein_id="HEX3136730.1"
                     /translation="PRAAWLRALLLERERVANHLGDLGALGNDAALAFGLSQFSRLRE
                     EWLRVSKAVFGHRLLMDSVVPGGTAVDVDPAAADRIARQCDVTQLQVKELRAIYDEHA
                     GLQDRFIGTGQVSPELAADLGLTGLAGRASGRAKDLRHDHPWPPYDALHVTIASRRGG
                     DVAARVAVRFAELFESLRLIRAIVTEMPEGEHRSEPRLSEDASVGAGWVEGWRGEVFV
                     ALELDAAGRGHAIRRCHCHDPSWQNWPVLEHAVIGNIVPDFPLINKSFNLSYSGHDL"
BASE COUNT          293 a          643 c          671 g          344 t
ORIGIN      
        1 gtcgggccgc gctttttatt ttattcacgc cctggttact ggatcgtgat ctgcttgctg
       61 ttggtcttgg ctaccttcgg cagcgtcagt tgcagcacgc cgtcgacgtg cgttgcctgg
      121 gtgccgttgg cgtcgaccgg ctcgggcaga cggatcgcgc gcgagagctt gccgaaattg
      181 cgctcttgcc aaagcgtctt gccgttggcg tcgatttcct ggctgaacga tgcgccgatc
      241 gtgacgttgc ggtcttcgac gttcacttcg atcttgtcct tgggtattcc gggcagctcg
      301 gccttgacga tgtaggcttc cggggtctcg acgacgtcca gcgcaatgct gcggctggtc
      361 gcgttcgcat cgccgttagc aagcactgcc ccgaacgcgc gatcgaacac tcgatccagc
      421 ggatcgaaat agcgaatcaa actggtcatt tgttcctctc cattcgtttt cgcggccact
      481 cggaaaaagg tttgagccgc gcacgaccgg taaatggcgg tcccccggcc gaattcaagg
      541 ggtgtcgggc gcgtccggcg accggagggt cggttcgacg gccgggcggt cggcctgcgc
      601 agcgaccggc ggcgcgtgcg cgctgatcgc cgccaggatg ccctgcagca gcgcccgcgg
      661 cgagggcggg cagccgggga tcgcgacatc gaccggaatc acgctggcca cgccgcccag
      721 actcgcatag ctctggccga agatcccgcc ggtgcagccg caatcgccga cggcgacgac
      781 cagcttgggc tcgggtgtcg cgtcgtaggt gcgcttgagc gcgatcgcca tgtgcttcga
      841 caccgggccg gtcaccagaa gcatgtccgc atggcgagga ctcgcgacaa agcgtatgcc
      901 gatgccctcg aggttgtagt acggattggt caacgcctgg atctccagct cgcacccgtt
      961 gcacgagccg gcgtcgacat gccggatcgt cagcgcgcgg ccaagcgtgc gcaggatcgt
     1021 cttttgcagc cgcgctacga cgatcatctc ctcggccggc gtcggcgcag tctcgctctt
     1081 gattccggta cgtgcgatct ggtggaggat gtgcagcatg actatagatc gtgcccgctg
     1141 taggaaaggt tgaacgactt gttgatcagc ggaaagtcgg gaacgatgtt gccgatcacc
     1201 gcatgctcga gcaccggcca gttctgccac gacgggtcat ggcagtggca gcgccgaatc
     1261 gcgtggccgc gcccggcggc gtcgagctcg agggcgacga agacctcgcc gcgccagccc
     1321 tcgacccagc cggcaccgac cgacgcgtct tcgctcaggc gcggttcgct gcggtgttcg
     1381 ccctccggca tctcggtcac gatcgcccgg atcaggcgca gcgattcgaa cagctcggcg
     1441 aagcggaccg cgacccgcgc cgcgacgtca ccgccgcggc ggctcgcgat cgtcacgtgc
     1501 agggcgtcgt agggcggcca cggatggtcg tggcgcaagt cctttgcccg gccgctggcc
     1561 cggcccgcga gcccggtcag gccgaggtcg gcggcgagtt cgggactgac ctggccggtg
     1621 ccgatgaagc gatcctgcag gccggcgtgc tcgtcgtaga tggcccgcag ctccttcacc
     1681 tgcagctgcg tgacatcgca ttgccgggcg atccggtctg cggccgcggg gtcgacatcg
     1741 accgcggtgc cccccggaac gacgctgtcc atcagcagcc ggtggccgaa gacggccttc
     1801 gacacgcgca gccactcttc gcgcagccgc gagaactggg acaggccgaa tgccagggcg
     1861 gcgtcgttgc cgagcgcgcc caggtcgccg aggtggttgg cgacgcgctc gcgctccagc
     1921 aggagcgcgc gcagccaggc cgcgcgcggt g
//
LOCUS       DATANS010000274         1800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_728665, whole genome shotgun sequence.
ACCESSION   DATANS010000274 DATANS010000000
VERSION     DATANS010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728665"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VHT22_03915"
     CDS             <1..254
                     /locus_tag="VHT22_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HEX3136731.1"
                     /translation="TPVSSTKGSTGHLLGAAGITEAIISLLAIEQGMLPGGMHTLALD
                     PALRCHYLTDNREGRVARVLTNSFGFGGSNCSLVLGSAG"
     gene            254..1048
                     /locus_tag="VHT22_03920"
     CDS             254..1048
                     /locus_tag="VHT22_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEX3136732.1"
                     /translation="MRVFVEGIGLVGPGLDGWQASRALLAGTAAYVQRPTVVVASDLL
                     PAAERRRTGLPVKLAMAAGREAFVNAQRNAATTATVFTSSSGDGENLHYICSTLASTE
                     REISPTRFHNSVHNAPAGYWSIATRSQAPSTSLCCRDASFAAGLLDAAAQIAVDGEPV
                     AVITYDQAYPQPLHAVRPLSASFGTAIVLTAQPTAQVFAALDVSYAPGANATPMTDSE
                     LDHLRATVPAARCLPLLAALAGGTPTVVDLAYGEHHHLHIEVTPWQ"
     gene            1039..1473
                     /locus_tag="VHT22_03925"
     CDS             1039..1473
                     /locus_tag="VHT22_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HEX3136733.1"
                     /translation="MAVTKAEIAGLIPHAGAMCLLDGVVEWDATHICSVSRSHHDMAN
                     PLRAGGELPALCGIEYAAQTMAVHGGLAGLVGAQPRAGYLISLRDVMCRKSRLDNLPG
                     DLMVDATQLMADQGRVIYEFTLRVGAAEILRGRATVVLDTAA"
     gene            1496..>1800
                     /locus_tag="VHT22_03930"
     CDS             1496..>1800
                     /locus_tag="VHT22_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3136734.1"
                     /translation="MKRALVTGGSGTIGAAICKRLAADGCHVYVHANGNLAHAERVSA
                     EITAAGGAARAVAFDVTSHAETAGALQELLVDGPIQIVVNNAGIHDDAVMPGMRPDQ"
BASE COUNT          303 a          616 c          599 g          282 t
ORIGIN      
        1 caactccggt gagttccacc aagggctcga ccggccacct gctcggcgcc gccggcatca
       61 cggaagcgat catcagcctg ctcgcgatcg aacagggcat gctgcccggc ggcatgcaca
      121 cgctcgctct cgatccggca ctgcgctgcc actatctgac cgacaaccgc gaaggtcgcg
      181 tcgcaagagt gctcactaat tcgttcggtt ttggcggcag caattgcagc ctcgtcctgg
      241 ggagcgccgg ctgatgcgcg ttttcgtcga gggcattgga ctggtcgggc cgggcctcga
      301 cggctggcag gcgagccggg cgctgctcgc gggcactgca gcttacgtgc agcggccgac
      361 ggtggtggtt gccagcgact tgctgcccgc cgcggaacgg cgccgcaccg gactgcctgt
      421 caaactcgcg atggcggcgg gccgcgaggc attcgtcaac gcgcagcgga acgccgcgac
      481 caccgcgacc gtgttcacgt cctcgtcggg cgacggcgag aacctgcact acatctgctc
      541 gacgctcgca tccaccgagc gggagatctc acccacccgg tttcataact cggtccacaa
      601 tgcgcccgcc ggctactgga gcatcgccac ccggtcgcag gcgccgtcga cgagcctgtg
      661 ctgtcgcgac gcaagtttcg ccgcgggcct gctcgatgcc gccgcgcaga tcgccgtgga
      721 cggcgaaccg gtcgccgtga ttacgtacga tcaggcttac ccgcagccgt tgcatgcagt
      781 ccggccgctc agcgcgagct tcggcacggc gatcgtgctg acggcgcaac ccaccgcgca
      841 ggttttcgcc gcgctcgacg tgagttatgc gcccggcgcc aatgcgacgc ccatgaccga
      901 tagcgagctg gatcatctgc gcgcgaccgt gcccgcggcg cgctgcctgc cattactcgc
      961 cgcactcgca ggcggaacgc caacggtcgt ggacctcgct tatggcgaac atcatcattt
     1021 gcacatcgag gtgacgccat ggcagtgacc aaagcggaaa tcgccgggct catcccgcat
     1081 gccggcgcga tgtgcctgct ggacggcgtg gtggaatggg acgcgacgca tatctgcagc
     1141 gtcagccgca gccaccacga catggcgaac ccgttacgcg ccggcggcga actgcccgcg
     1201 ctgtgcggaa tcgagtacgc ggcgcagacg atggccgttc atggagggct cgccggtctc
     1261 gtgggcgccc agccgcgcgc cggctatttg atcagcctgc gcgatgtcat gtgccgcaaa
     1321 agccggctcg acaatctgcc cggcgatctc atggtcgatg cgacccaact catggcggat
     1381 cagggacgcg tgatttacga attcacgctg cgggtaggcg cggcggaaat cctgcgcggg
     1441 cgcgccaccg tggtgctgga cactgcggca tgacaccaca caagggagac ctcggatgaa
     1501 acgcgcgctc gtcaccggcg gcagcgggac catcggcgcc gccatctgca agcggctcgc
     1561 tgcggatggc tgccacgtct atgtgcacgc caacggcaat ctcgcccacg cagagcgcgt
     1621 gagcgcggag ataacggcgg ccggcggtgc ggcgcgcgcg gtcgctttcg acgtgacctc
     1681 gcacgccgaa accgccggcg cgctgcagga gttgctggtg gacggcccga tccagatagt
     1741 cgtcaacaat gccggcatac acgacgatgc ggtgatgccg ggcatgcgcc cggatcaatg
//
LOCUS       DATANS010000275         2816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_308574, whole genome shotgun sequence.
ACCESSION   DATANS010000275 DATANS010000000
VERSION     DATANS010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308574"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VHT22_03935"
     CDS             complement(<1..559)
                     /locus_tag="VHT22_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HEX3136735.1"
                     /translation="MKVWAINGVTPVVDPSSFVHASAVLIGDVIVGPGCYVGPAASLR
                     GDFGRIELRAGSNVQDSCVMHGFPSTDTVVEDNGHIGHGAILHGCVVKANALVGMNAV
                     INDNAVIGESAIVAAMAFVKAGMIVPPRTLVAGVPARIVRELTEQELAWKVEGTQSYQ
                     ELTRRSLATMLATDALTSVEPGRRRI"
     gene            complement(612..>2816)
                     /gene="purL"
                     /locus_tag="VHT22_03940"
                     /gene_synonym="purI"
     CDS             complement(612..>2816)
                     /gene="purL"
                     /locus_tag="VHT22_03940"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEX3136736.1"
                     /translation="SLDRFRAICERERCPFAVVGSTTAEPRLAVTDRALGSQPVDMDL
                     GVLLGRPPRMTREAVHLERELPPFDARQIELADAAFRVLQLPSVGDKTFLVTIGDRTV
                     GGLSARDPMVGPWQVPVADAAATLLDFDGYSGEAMAIGERTSLALIDAPASGRMAIGE
                     ALTNIAAAAIDQLRDVKLSANWMAPAGHPGEDAALYDTVRAVALDLCPALGVSIPVGK
                     DSMSMRTSWRDGNVDKAVTAPVSLIVSAFAAIADARRVLTPELRTDVGSVLLLVDLGN
                     GKNRLGGSALAQVYGQLGNVAPDVDDPAALAAFFATVQALHAQNKLLAYHDRSDGGLF
                     VTLVEMALAARCGLEICIDELPGSAAEALFNEELGAVLQVRAADRHDVVAAFEAAGLR
                     CICVGEPTSNGRIRILGDSSTQFEASRIELHRAWSATTHALQRMRDNPASADQEYERI
                     SDADDPGLAPMLGFDPSEDVAAPYIAAGVRPRVAVLREQGVNGQVELAAAFDRAGFAA
                     YDVHMSDIIAGRETLAGFAGLAAGGGFSYGDVLGAGEGWAKSILFNARARDAFAAFFA
                     RAETFALGICNGCQMMSNLHELIPGSEHWPHFVRNRSEQFEARLILLEVQPSPSLFFA
                     GMEGSRIPVALAHGEGYAEFRDAGQQAAAEALVTLRFVDNRGAATERYPYNPNGSPQG
                     ITGLTTADGRFSILMPHPERVFRSVQMSWHPGDWGEDSPWMRMFRNARRWIG"
BASE COUNT          477 a          974 c          921 g          444 t
ORIGIN      
        1 cgatgcgccg gcgtcccggc tcgacgctgg tcagcgcgtc ggtcgcgagc atcgtcgcga
       61 ggctgcggcg cgtcagctcc tgataactct gcgtcccctc gactttccag gcaagctcct
      121 gttcggtcag ctcgcggacg atccttgccg gcacgcctgc gaccagcgtg cgcggtggca
      181 cgatcatgcc ggccttgacg aatgccattg ccgccacaat ggccgattcg ccgatcactg
      241 cgttgtcgtt gatcacggcg ttcatgccca ccagcgcgtt ggccttgaca acgcaaccat
      301 gcaggatcgc gccgtggccg atatgcccgt tgtcttcgac cacggtgtcg gtgctgggaa
      361 aaccgtgcat cacgcacgaa tcctgcacgt tcgagccggc gcgaagctcg atgcggccaa
      421 agtcgccgcg cagcgatgcc gccggtccga catagcagcc ggggccgacg atgacgtcgc
      481 cgatcagcac ggcgctggcg tgcacgaacg agctcggatc gacgaccgga gtcacgccgt
      541 tgatagccca gactttcatc ggcgttgtat cttccggcag cactcgcgcg gtccgaatcg
      601 catgtccgcc ctcatccgat ccagcgccgc gcgttgcgga acatgcgcat ccacggcgag
      661 tcctctcccc agtcgcccgg atgccacgac atctgcacgc tgcggaatac gcgctccgga
      721 tgcggcatca atatggaaaa acgcccgtcg gccgtcgtta acccggtgat gccctgcggc
      781 gacccgttcg gattgtacgg gtatcgctcg gtcgccgcac cgcggttgtc gacgaagcgc
      841 aaggtcacca gcgcttcggc cgcggcctgc tgcccggcat cgcggaactc ggcgtagcct
      901 tcgccatggg cgagcgcgac cggtatccgg ctgccttcca ttccggcgaa aaacagcgat
      961 ggcgaaggct gcacttcgag caggatcagt cgcgcctcga actgctccga gcgattgcgg
     1021 acgaagtgcg gccagtgctc gcttccgggg atcagctcgt gcaggttgct catcatctga
     1081 cagccgttgc agatgccgag cgcaaaggtc tcggcgcgcg cgaagaatgc ggcgaacgca
     1141 tcgcgcgcgc gcgcattgaa caggatcgat ttggcccagc cttcgccggc gccgagcacg
     1201 tcgccatagg agaatccccc gccggccgcg aggcccgcga atccggcgag cgtctcgcgg
     1261 ccggcgatga tgtcgctcat gtgcacgtcg taggccgcga agccggcgcg atcgaaagcg
     1321 gctgcgagct cgacctggcc gttgacgccc tgctcgcgca gcaccgccac gcgtggtcgc
     1381 acgccggcgg cgatgtaggg cgcggcgacg tcctcggacg gatcgaagcc gagcatcggc
     1441 gcgaggcccg gatcgtcggc gtcggagatc cgctcgtatt cctggtcggc ggacgccggg
     1501 ttatcgcgca tccgctgcag cgcatgcgtc gttgccgacc aggcccgatg caattcgatg
     1561 cgggaagcct cgaactgcgt cgaggaatcg ccgaggatcc gtatacggcc attggacgtc
     1621 ggctcgccga cacagatgca gcgcagtccg gcggcttcga acgcagcgac aacgtcgtgc
     1681 cggtcggcgg cgcgaacctg cagcacggcc ccgagctcct cgttgaacag cgcttcggcg
     1741 gcagaacccg gcagctcgtc gatgcagatc tcgaggccgc aacgcgcggc gagcgccatc
     1801 tcgaccagcg tcacgaacag gccgccgtcc gagcggtcgt gatacgccag caacttgttc
     1861 tgcgcatgca gtgcctgcac ggtcgcgaag aatgcggcga gcgccgcagg atcgtcgacg
     1921 tcgggcgcga cattgccgag ctgaccgtat acctgagcca gcgccgagcc gcctagccgg
     1981 ttctttccgt tgccgagatc gaccagcaac agcaccgagc cgacgtcggt acgaagctcc
     2041 ggcgtcagca cgcggcgcgc gtcggcaatg gcggcgaacg ccgatacgat cagcgacacc
     2101 ggcgcggtta ccgccttgtc gacgttgccg tcgcgccacg aagtccgcat cgacatcgaa
     2161 tccttgccga caggaatact cacccccagc gccggacaaa gatcgagggc gacagcgcgc
     2221 accgtgtcgt acagcgcggc gtcctcgccc ggatgtcccg ccggtgccat ccagttggcc
     2281 gagagcttca cgtcgcgcaa ctggtcgatc gcggcggcgg cgatgttggt caatgcttcg
     2341 ccgatcgcca tgcgtccaga cgccggcgcg tcgatcagcg ccagcgacgt tcgttcgccg
     2401 attgccatcg cttcgccgga atagccgtcg aaatcaagca gggtcgcagc ggcgtcggcc
     2461 accggcacct gccatggccc gaccatcggg tcgcgcgcgc tcaagccgcc gacggtccga
     2521 tcgccgatcg tcaccagaaa ggtcttgtcg ccaacgctgg gcagttgcag cacgcggaat
     2581 gccgcgtcgg cgagctcgat ctggcgcgca tcgaatggcg gaagctcgcg ctcgagatgc
     2641 accgcttccc gcgtcattcg cggcggtcta cccagcagca cgcccaagtc catgtcgacc
     2701 ggctggctgc ccagggcgcg gtcggtgacg gccaatcgag gctcggcggt cgtgctgccg
     2761 acgacggcga atggacagcg ctcgcgctcg cagatcgcgc gaaagcgatc cagcga
//
LOCUS       DATANS010000276         1695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_266273, whole genome shotgun sequence.
ACCESSION   DATANS010000276 DATANS010000000
VERSION     DATANS010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266273"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..>1695
                     /locus_tag="VHT22_03945"
     CDS             69..>1695
                     /locus_tag="VHT22_03945"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX3136737.1"
                     /translation="MYRYDHYDQRLVDERVAQFRDQTRRFLAGELTEDEFRPLRLRNC
                     LYIQKYAPMLRISVPYGLLSSTQMRKLAHIGRKYDRGYGHFTTRQNIQFSWPRLEDVP
                     DILGELATVEMHAIQTSGNCVRNITSDHFAGIAADEIIDPMPYSELFRQWSTFHPEFA
                     YLPRKFKIAVSGSATDRAATLVHDIGVHAVRNEAGNIGFRVLVGGGLGRTPVIGHVIR
                     EFLPREEILNYLDAILRVYNRFGRRDNIYKARIKILVKEMTLEVFTRHVESEWQRVQG
                     GPATVPDEEIQRIAAFFAPPPYERLPGDDAGFRAAVADNRAFANWVKRNVFPHKVPGY
                     AAVTLSLKKTGVAPGDATSDQMDMVADLADAYSFGEIRISHEQNLIFAHVRQAALLAL
                     WGELKALGFATPNIGLLTDIICCPGGDFCSLANAKSIPIAEAIQRRFDDLDYLHDIGE
                     INLNISGCMNACGHHHVGNIGVLGVDKGGAEWYQVSIGGDQGEHASLGKVIGPSFAAR
                     EMPDVVAKLLDTYIERRHADEQFIDTVRRIGIEP"
BASE COUNT          323 a          560 c          519 g          293 t
ORIGIN      
        1 cgccactcat tccttgtacg cataacgcag cccaactacg ctggcggcta gaaccctgcg
       61 accagctcat gtaccgatac gaccactacg atcaacgcct cgtcgacgag cgtgtcgccc
      121 agtttcgcga ccagacgcgg cgttttctcg ccggcgagct gacggaagac gagttccggc
      181 cgctgcgcct gcggaactgc ctgtacatcc agaaatacgc gccgatgctg cggatttcgg
      241 ttccgtacgg cctgttgtcg tcgacccaga tgcgcaagct cgcccacatc ggccgaaagt
      301 atgacagagg ctacgggcat ttcaccacgc gccagaacat ccagttcagc tggccgcggc
      361 tcgaggacgt tccggacatc ctgggcgagc tggccacggt ggaaatgcac gcgatccaga
      421 ccagcggcaa ctgcgtgcgc aacatcacct ccgaccactt tgccggcatc gcggccgacg
      481 aaatcatcga cccgatgccg tacagcgagc tgttccggca atggtcgacg ttccatcccg
      541 agttcgccta cctgccgcgc aaattcaaga tcgcggtgtc gggctctgcg accgatcgcg
      601 cggcgacgct ggtgcacgat atcggcgtgc acgcggtacg caacgaggcg ggaaacatcg
      661 gattccgcgt gctggtcggc ggcggactcg ggcgcacacc ggtgatcggt cacgtcatcc
      721 gcgaatttct gccgcgcgaa gaaatcctca attacctgga cgcgatcctg cgcgtctaca
      781 accgcttcgg ccggcgcgac aacatttaca aggcccgcat caagatcctg gtcaaggaga
      841 tgacgctcga ggtcttcacg cggcacgtcg aatcggaatg gcagcgtgtt caaggcgggc
      901 cggcgacggt gccggacgag gaaatccagc gcatcgcggc gttcttcgcg ccgccgccct
      961 acgagcgttt gcccggcgac gacgcaggtt ttcgcgcggc ggtcgccgac aaccgggcgt
     1021 tcgccaactg ggtcaagcgc aacgtcttcc cgcacaaggt tccgggctat gccgccgtca
     1081 cgctgtcgct gaagaagacc ggcgtggcgc cgggcgacgc gacatcggac cagatggaca
     1141 tggtcgccga cctggccgac gcgtacagct tcggcgaaat tcgcatttcg cacgagcaga
     1201 atctgatctt cgcgcacgtc aggcaggctg ccctgctcgc actctggggc gagctcaagg
     1261 cgctcggctt cgcgacgccg aacatcggct tgctcaccga catcatctgc tgccccggcg
     1321 gcgacttctg ctccctggcc aatgccaagt cgataccgat cgccgaagcg atccagcggc
     1381 gattcgacga tctcgattac ctgcacgaca tcggcgagat caacctcaac atctcggggt
     1441 gcatgaacgc ctgcggccac caccacgtcg gcaatatcgg ggtgctgggc gtcgataagg
     1501 gcggcgccga gtggtatcag gtgtcgatcg gcggcgacca gggcgagcat gcgtctctgg
     1561 gcaaagtaat cggtccgtcg tttgccgcgc gcgagatgcc tgatgtcgtc gcgaagctcc
     1621 tcgataccta catcgagcgg cgccacgctg acgagcagtt catcgatacc gtgcggcgaa
     1681 tcggcatcga gccgt
//
LOCUS       DATANS010000277         3678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_686375, whole genome shotgun sequence.
ACCESSION   DATANS010000277 DATANS010000000
VERSION     DATANS010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686375"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VHT22_03950"
     CDS             <1..216
                     /locus_tag="VHT22_03950"
                     /inference="COORDINATES: protein motif:HMM:NF015424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX3136738.1"
                     /translation="GLSVLNVLPATLVAIATEEGPIVDVQLRVGKLPLTARITRRSMH
                     ELALREGQQVYALIKAVSLDRRSVGYA"
     gene            complement(220..567)
                     /locus_tag="VHT22_03955"
     CDS             complement(220..567)
                     /locus_tag="VHT22_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModE family transcriptional regulator"
                     /protein_id="HEX3136739.1"
                     /translation="MKLRTEFRLRIRRGDDIAVGPGKIDLLEAIDTAGSITAAARSLG
                     MSYRRAWLLVDTMNRCFRIPVVEAEAGGKRGGGARLTRLGRDVIERYRRIERNAARAS
                     AADIRALTRKLAG"
     gene            complement(590..2404)
                     /gene="glmS"
                     /locus_tag="VHT22_03960"
     CDS             complement(590..2404)
                     /gene="glmS"
                     /locus_tag="VHT22_03960"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX3136740.1"
                     /translation="MCGIVGAVARDNVVPILIEGIRRLEYRGYDSTGLAVINGGLHRL
                     VSTARVADLAAQANATQISGNTGISHTRWATHGAPTSANAHPHVSSGEIAVVHNGIIE
                     NFEPLRARLQQQGYAFVTQTDTEVIAHLIHAHYDGDLLAAVAKAVAEFHGAYAIAAMT
                     TREPGRVVGARAGSPLLVGVGEHDHFLASDASALAPVTRRVVYLEEGDIADIGREGYA
                     IYDAAGVRVAREVVSVKTSGDAVELGPYSHFMQKEIFEQPRAIADTLESVGGIGPELF
                     GAAAAEVFAGVNEVRVLACGTSYYSGMVGRLWLEAVAGIPAQAEIASEYRYRDSVPNP
                     DALVVVISQSGETADTLSALKHAKALGHRHTLAICNAPTSSMVRQTALTYLTRAGVEI
                     GVASTKAFTTQLTALFLLTLTLAKQRGRLDAAQESRWLQDLRHLPAAIQAVLALEPSI
                     IAWAERFAKKENALFLGRGLHYPIALEGALKLKEISYIHGEAYPAGELKHGPLALVDA
                     NMPVVAIAPNDALLEKLKSNLQEVRARGGELYVVADADSHIPSSDGIHVLRLPEHAGL
                     LSPIVHTIPLQLLAYHTAVLRGTDVDKPRNLAKSVTVE"
     gene            complement(2409..>3678)
                     /gene="glmU"
                     /locus_tag="VHT22_03965"
     CDS             complement(2409..>3678)
                     /gene="glmU"
                     /locus_tag="VHT22_03965"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX3136741.1"
                     /translation="LAHVLSTARKLEPRAICVVDSGGEVRERFADPDLTWARQDPPRG
                     TGDALKCALSELGADGVTLVMFGADPLARAETLRRVAERAQQGILSLLTVELDDPSGY
                     GRIVRGADGKVAAIVEHKDATPEQRAIREINTGIMAAPTAAFARWLPRIGNRNAGGEY
                     YLTDVIALAVADGIPVETTHPATADETLGINSKRDLAMLERIYQRGQADALLDAGVTL
                     ADPARIDVRGTLACGRDVTIDIGCIFEGDVALGDAVSVGAHSILRDVKIGTATAIAPY
                     SHIDGADIGARCRIGPYARIRPGTRLADEVHIGNFVEVKASAVGERSKANHLAYVGDT
                     TVGRDVNIGAGTITCNYDGANKHRTVIEDDVHIGSDTQLVAPVTIGRGATIGAGTTVW
                     RDVEPGVLALNQKTQTAKAGYQRPRKKPKE"
BASE COUNT          556 a         1254 c         1222 g          646 t
ORIGIN      
        1 ggattgagcg tgctcaatgt cctgccggcg acgctggtcg cgatcgccac tgaagaaggc
       61 ccgatcgtcg acgtgcagct gcgcgtcggc aagctgccgc tcaccgcgcg catcactcgt
      121 cgttcgatgc acgagctcgc acttcgcgaa gggcagcaag tctatgcgct gatcaaggcg
      181 gtatcgctcg accggcgcag cgtcggctac gcctagacgc tagcctgcga gctttcgtgt
      241 caacgcgcgg atgtcggccg cgctggcgcg cgccgcgttt cgttcgatcc ggcggtagcg
      301 ctcgatcacg tctcgcccaa gccgggtaag gcgcgcgccg ccgccgcgct ttccgccggc
      361 ttcggcctcg accacaggaa tgcgaaagca tcgattcatc gtgtcgacca gaagccacgc
      421 acggcggtac gacatgccga gcgagcgtgc cgccgcggtg atcgagccgg cggtgtcgat
      481 cgcttcgagc aggtcgatct tgccagggcc gaccgctatg tcatcgccgc gacggatgcg
      541 caggcgaaac tcggtacgga gcttcatcgg cgactgcggc cggtgtttct cactccaccg
      601 ttaccgactt cgccagattc cgcggcttgt ccacgtcggt gccgcgcagc acggcggtgt
      661 ggtaggcgag cagctgcagc gggatggtgt gcacgatcgg cgacagcagg cccgcatgct
      721 cgggcaggcg cagcacgtgg atgccgtcgc tcgacggaat gtggctgtcg gcgtcggcga
      781 cgacatagag ctcaccgccg cgggcacgca cttcctgcag gttcgacttg agcttctcca
      841 gcagcgcgtc gttcggggcg atcgccacga ccggcatgtt ggcgtcgacc agcgcgagcg
      901 ggccgtgttt caactcgccg gccggatacg cctcgccatg gatgtatgag atctccttga
      961 gcttcagcgc gccttcgagc gcgatcggat agtgcaggcc ccgcccgaga aaaagcgcgt
     1021 tttccttttt ggcgaagcgt tcggcccagg cgatgatcga cggctcgagc gccagcaccg
     1081 cctggatcgc cgccggcagg tggcgcaggt cctgcagcca tcgcgattcc tgcgcggcgt
     1141 cgaggcgtcc ccgctgcttg gccaaagtca gcgtcagcag gaacaacgcg gtcagttgcg
     1201 tcgtgaacgc cttcgtcgag gcaacgccga tctcgacgcc tgcccgggtc agataggtaa
     1261 gcgcggtctg ccgcaccatc gagctcgtcg gagcgttgca gatcgccagc gtgtggcgat
     1321 ggccgagcgc cttggcgtgc ttcaatgccg acaaggtgtc cgccgtctcg cccgactgcg
     1381 aaatcacgac gaccagcgca tcgggattgg gcacgctgtc gcgatagcgg tattcgctgg
     1441 cgatttcggc ctgcgccggt atgccggcaa cggcctcgag ccacagccgc ccgaccatgc
     1501 cagagtagta gcttgtgccg caagcgagca cgcgcacctc gttgacgccg gcgaaaactt
     1561 cggccgccgc ggctccgaac agctcgggcc cgatgccgcc gacgctttcc agggtgtcgg
     1621 cgatggcgcg cggttgctcg aagatttcct tctgcatgaa atggctgtac gggccgagct
     1681 ccaccgcatc gcccgaagtc ttgaccgaca ccacctcccg cgcgacgcgc acgccggcgg
     1741 cgtcatagat cgcgtatccc tcgcgtccta tgtcggcgat gtcgccttcc tcgagataga
     1801 cgacgcgcct ggtgaccggc gccagcgcgc tcgcgtcgga ggcgaggaag tgatcgtgct
     1861 cgccgacgcc aaccagcagc ggactgccgg cccgcgcgcc gacgacgcga cccggctcac
     1921 gcgtggtcat cgcggcgatc gcgtaggctc cgtggaactc ggcgaccgcc ttggccaccg
     1981 cggcgagcag atcgccgtcg taatgcgcat ggatcagatg cgcgatgacc tcggtgtcgg
     2041 tctgagtgac gaatgcgtat ccctgctgct gaagccgtgc gcgcagcggc tcgaagttct
     2101 cgatgatccc gttatgcacg accgcgatct cgccgctgct gacgtgcgga tgcgcgttgg
     2161 ccgaggtcgg cgcgccgtgc gtcgcccagc gcgtgtgcga aatgccggtg tttccgctga
     2221 tctgggtcgc gttcgcctgc gccgcgagat cggcaacgcg cgccgtgctg accagccggt
     2281 gaaggccgcc gttgatgacc gcgagcccgg tcgagtcgta accccggtat tcgagccggc
     2341 ggatgccctc gatcaggatc ggaacgacgt tatcgcgcgc gaccgcgccg acgatgccgc
     2401 acatgatgct attccttcgg tttcttccgc gggcgctggt agcccgcctt ggctgtctgc
     2461 gtcttctggt tgagcgcgag gacgccgggc tcgacgtcgc gccacacggt cgtgccggcg
     2521 cctatcgtcg cgccgcggcc aatcgtgacc ggggccacca gctgcgtatc ggacccgatg
     2581 tggacgtcgt cctcgatcac cgtccgatgc ttgttcgcgc cgtcgtagtt gcaggtgatc
     2641 gtgcccgcac cgatgttgac gtcgcgcccg accgtcgtgt cgcccacgta ggccaggtgg
     2701 ttcgccttgc tgcgctcgcc gacggcgctc gccttcacct cgacgaagtt gccgatgtga
     2761 acctcgtccg ccagacgcgt gcctggccgg atgcgcgcgt acgggccgat ccggcaacgc
     2821 gccccgatgt cggcgccgtc gatatgcgaa tacggcgcga tcgccgtcgc cgtgccgatt
     2881 ttcacatctc tgagaatgct gtgcgcgccg acgctgaccg catcgccgag cgccacgtcg
     2941 ccttcgaaaa tgcagccgat gtcgatcgtt acgtcgcggc cgcaggccag cgtgccgcgc
     3001 acatcgatgc gcgccggatc ggccagggtc actccggcat cgagcagcgc gtccgcctgc
     3061 ccgcgttgat agatccgctc gagcatcgcc aggtcgcgct tgctgttgat gcccagcgtc
     3121 tcgtccgcgg tcgccggatg cgtcgtttcg actggaatgc cgtcggcgac ggccagtgcg
     3181 atcacatcgg tcagataata ttcgccgccg gcattccgat taccgattct cggcagccag
     3241 cgcgcgaacg cggcggtcgg cgccgccatg atgccggtgt tgatctcccg gatcgcgcgc
     3301 tgttccggtg tcgcatcctt gtgctcgacg attgccgcga ccttgccgtc ggcgccgcgc
     3361 acgattcgtc cataaccgga cgggtcatcg agctcgacgg tgagcagcga aagaatgcct
     3421 tgctgggcgc gctcggcgac ccgacgcaag gtctctgccc gcgccagcgg atcggcgccg
     3481 aacatgacga gcgtgacgcc gtcggcgccc aattccgaga gcgcgcactt caatgcatcg
     3541 ccggtgccgc gcggcgggtc ctgtcgcgcc caggtcaagt cgggatcggc aaagcgctcg
     3601 cgcacctcgc cgccggaatc gacgacgcag atcgctcgcg gctctagctt gcgcgcggtc
     3661 gacaatacat gcgcgagc
//
LOCUS       DATANS010000278         4067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_560886, whole genome shotgun sequence.
ACCESSION   DATANS010000278 DATANS010000000
VERSION     DATANS010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560886"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VHT22_03970"
     CDS             <1..1050
                     /locus_tag="VHT22_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX3136742.1"
                     /translation="GILKEVAQPPLAIKDKMASRIKVISRLDISEKRVPQDGRMKLTL
                     SKSRAIDFRVSTLPTLYGEKIVLRILDAAGVKLGIEALGYEPDQQQIILDAIKRPYGM
                     ILVTGPTGSGKTVSLYTCLNILNQPGVNIATAEDPAEIQLAGVNQVNINEKQGLNFAN
                     ALRAFLRQDPDIIMVGEIRDLETADIAIKAAQTGHLVMSTLHTNDAPATLMRLANMGV
                     PAFNIASSVILITAQRLGRKLCTVCKKAEEIPPEALLQAGFTEEDIDGTWQCFGPVGC
                     DHCFGTGYKGRVGIYEVMPITDDMRQIIMRAGNALDIAEQARKDGVKNLRQSGLLKVK
                     QGMTSLEEIEAVTNE"
     gene            1075..2313
                     /locus_tag="VHT22_03975"
     CDS             1075..2313
                     /locus_tag="VHT22_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX3136743.1"
                     /translation="MATTATVQRRAQIRDVKEYTYLWEGTDRNNRQVRGEMRAASETV
                     VTTTLRRQGIRIAKLRRQSFRGGRRVSEKDITFFTRQLATMLKAGVPLLQAFEIVARG
                     HSNARFARLMMDIKGRVEAGSSLSQAFREYPAHFDALYCNLVHAGETAGMLDGILERL
                     ATYKEKILAIKSKIKSALFYPVSVVVVAIVVIWVIMIWVIPAFKQVFTSFGANLPAPT
                     LIVMGISDFFVSYWWLMAAIGVGTVFGFLFLQRRSAAFRYFLDRISLKLPVIGAILEK
                     ATIARWTRTLATMFAAGVPLVESLDAVAGASGNAVYIAGTRKIQTQVSTGTSLTNAMH
                     NTGLFPTMVLQMTQIGEESGSLDGMLSKVAEFFEREVDDAVAALSSLLEPIIIVFLGV
                     VIGGLVVAMYLPIFKLGAVV"
     gene            2465..3322
                     /locus_tag="VHT22_03980"
     CDS             2465..3322
                     /locus_tag="VHT22_03980"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX3136744.1"
                     /translation="MPFAFAFDSVALLVLAAVVGLCVGSFLNVVIHRLPKMLERGWRE
                     QCAELAGETPAEAPAYNLVVPRSQCPSCGHQIGALENIPVISYLVLRGHCRACHAPIS
                     ARYPIVELLAAFLAVAAIVRFGFTPAALAACVFLWMLLALTLIDFDTQLLPDNLTLPL
                     LWTGLLANVLGAAPIANLRDAVIGAIAGYLTLWLVYWLFKLIRGKEGMGYGDFKLLAA
                     LGAWLGWQMLPLIVLLSSVVGAAIGISLVVFKGRDHQTPLAFGPYLAIAGGIALFFGR
                     SLIAIYLPA"
     gene            3341..3967
                     /gene="coaE"
                     /locus_tag="VHT22_03985"
     CDS             3341..3967
                     /gene="coaE"
                     /locus_tag="VHT22_03985"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989203.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX3136745.1"
                     /translation="MPFVVGLTGGIGSGKSAVANAFASLGVEIVDADLLAHRLSAPGQ
                     PGYAAIREAFPDVALTPDGEIDRAQLRNRVFADPAARVRLEAALHPLIAATAKREIAA
                     WRGAYGVTVVPLLLERGKLASAVDRILVVDCPEDAQVLRVVARSGMTPAEVRAIMATQ
                     ASRQARLADADDVIDNAGPREAIAPQVAKLDARYRELAASPTSRAIAP"
BASE COUNT          708 a         1343 c         1323 g          693 t
ORIGIN      
        1 gggatcctga aggaagtcgc gcagccgccg ctggcgatca aggacaagat ggcgtcgcgg
       61 atcaaggtca tctcgcggct cgacatctcg gaaaagcgcg tgccgcagga cgggcggatg
      121 aagctgacgc tgtcgaaaag ccgcgcgatc gacttccggg tcagcaccct tccgactctt
      181 tacggcgaaa agatcgtgct gcgtatcctc gacgccgcag gcgtcaagct cggcatcgag
      241 gcgctcggct acgagcccga ccagcagcag atcatcctcg atgcgatcaa gcgtccctac
      301 ggcatgatcc tggtgaccgg gccgacggga agcggaaaga cagtgtcgtt gtacacgtgc
      361 ctgaacatcc tgaaccagcc tggcgtgaat attgcaacgg ccgaggaccc ggccgaaatc
      421 cagctggccg gcgtcaacca ggtcaacatc aatgaaaagc aaggtctcaa tttcgccaac
      481 gcgctgcgcg ccttcctgcg gcaggatccc gacatcatca tggtcggcga aatccgcgac
      541 ctggaaaccg ccgacatcgc gatcaaggcc gcgcagaccg gtcacctggt gatgtcgacg
      601 ctgcacacca acgacgcccc cgcgacgctg atgcggctcg ccaacatggg cgtgcccgca
      661 ttcaacatcg cctcgagcgt catcctgatt accgcccagc ggctggggcg caagctctgc
      721 acggtctgca agaaggcgga ggaaattccg cccgaagcgc tgctgcaagc cggatttacc
      781 gaagaggaca tcgacggaac ctggcagtgc ttcggtccgg tcgggtgcga ccattgcttc
      841 ggcaccggct acaagggccg tgtcggaatc tacgaagtga tgccgatcac cgacgacatg
      901 aggcagatca tcatgcgggc cggcaacgcg ctcgatatcg ccgagcaggc gagaaaagac
      961 ggcgtaaaga atctccggca atcgggactc ctcaaggtca agcagggcat gacctcgctc
     1021 gaggagatcg aggccgtaac caacgaatag cgcacaaccg cgacgggaat catcatggcg
     1081 accaccgcaa ccgtacaacg cagagcccag atcagggatg tcaaggaata cacctacctg
     1141 tgggagggca ccgaccgcaa caaccgccag gtgcgcggcg aaatgcgcgc ggcatcggag
     1201 acggtcgtca cgacgacgct gcggcgccag ggcatacgga tcgcgaagct ccgccgccag
     1261 tcgttccgcg gcggccggcg cgtcagcgaa aaggacatca cgttcttcac ccgccagctg
     1321 gcaacgatgc tcaaggccgg cgtgccgctg ctgcaggcgt tcgagatcgt ggcgcgcggc
     1381 cacagcaacg cccgcttcgc gcggctgatg atggacatca agggccgggt cgaagccggc
     1441 tccagcctgt cgcaggcgtt ccgcgagtat ccggcccact tcgatgcgct gtactgcaac
     1501 ctggtccacg ccggcgagac cgcgggcatg ctggacggca tcctcgagcg gctggcgacc
     1561 tacaaggaaa agatcctggc gatcaagagc aagatcaagt ccgccttgtt ctatccggtt
     1621 tcggtcgtcg tcgttgcgat cgtcgtcatc tgggtgatca tgatctgggt gattccggcg
     1681 ttcaagcagg tcttcaccag cttcggcgcc aacctgccgg cgccaaccct gatcgtgatg
     1741 ggcatctccg atttcttcgt ctcctactgg tggctgatgg cggcgatcgg agtcggcacg
     1801 gtcttcggct tcctgttcct gcagcgccgc tcggcggcat tccgctattt cctcgaccgg
     1861 atctcgctca agctgccggt gatcggcgcg atcctggaga aggcgacgat cgcgcgctgg
     1921 acgcgcaccc tggcgacgat gttcgccgcc ggcgtgccgc tggtcgaatc gctcgacgcc
     1981 gtcgccggcg cctcgggcaa tgccgtctac atcgccggca cgcgcaagat ccagacgcag
     2041 gtcagcaccg gcacgagcct gaccaacgcc atgcacaaca ccgggctgtt cccgacgatg
     2101 gtgctgcaga tgacgcagat cggcgaagag tcgggctcgc tcgacggcat gctgtccaag
     2161 gttgccgagt tcttcgagcg cgaagtcgac gatgcggtcg cggcgctgtc gagcctgctt
     2221 gagcctatca tcatcgtgtt cctgggcgtc gtgatcggcg gactcgtcgt cgcgatgtac
     2281 ctgccgatat tcaagcttgg cgccgtcgtc tgacggggac gcgggaagcg aagcgcaatc
     2341 gtgccgttcc gccggatgcg gccaccggcg gacggcgacc gttcgcggac ggcagcatgc
     2401 cgcccgcatc gtgtcggagc cgccgccaac gatcaccccg gcttgccgcg cgcgaccttg
     2461 accgatgccc ttcgcattcg cattcgatag tgtcgccctc ctggtgctgg ccgcggtcgt
     2521 cggactgtgc gtcggcagct tcctgaacgt cgtcatccac cgccttccga aaatgctcga
     2581 gcgcggctgg cgcgagcaat gcgcggagct cgccggcgaa acgccggccg aagcgcccgc
     2641 ctacaacctg gtcgtgccgc gctcgcagtg cccatcgtgc ggacaccaga tcggcgcgtt
     2701 ggaaaacatt ccggtcatca gctacctcgt gctgcgcggg cactgtcgcg cctgccatgc
     2761 gccgatctcg gcgcgctatc cgatcgtcga gctgctggcc gcgttcctgg cggtcgcggc
     2821 catcgtgcgt ttcggcttca cgccggcggc gctggccgcc tgcgtgttcc tgtggatgct
     2881 gctggcgctg accttgatcg acttcgatac gcaactgctg cccgacaacc tgaccttgcc
     2941 gctgctgtgg acggggctgc tggcgaacgt cctcggcgct gcgccgattg cgaatctgcg
     3001 cgatgcggtg atcggcgcga tcgccggcta cctgacgctc tggctggtct actggctgtt
     3061 caagctgatt cgcggcaagg agggcatggg ctacggcgat ttcaagctgc ttgccgcgct
     3121 gggcgcgtgg ctcggctggc agatgctgcc gctgatcgtg ctgctgtcgt cggtcgtcgg
     3181 cgcggcgatc ggcatttcgc tggtcgtgtt caagggacgc gatcaccaga caccgctggc
     3241 gttcggaccg tacctggcga tcgccggcgg gattgcgctt ttcttcggcc gatcgctgat
     3301 cgcgatctac ctgcccgcgt aggggaattg agggacgctg atgccattcg tcgttgggtt
     3361 gaccggagga atcggcagcg gcaaatccgc ggtggcgaac gcgttcgcct cgctcggtgt
     3421 cgagatcgtc gacgccgatc tgctggcgca tcggctgtcg gcgccgggac aaccgggtta
     3481 cgcggcgatt cgcgaagcgt tccccgatgt cgcgctgaca ccggacggcg agatcgaccg
     3541 cgcgcaactg cgcaaccgcg tcttcgccga ccccgcggcg cgcgtgcgcc tcgaagctgc
     3601 gttgcatccg ctgatcgccg ccacggcgaa acgtgagatc gcggcctggc gcggagccta
     3661 cggtgtcacc gtcgttccac tcctgctcga gcgcggcaag ctcgcgtcgg cggtcgaccg
     3721 gatactcgtc gtcgactgcc cggaagacgc gcaggtgtta agagtcgtcg cgcgcagcgg
     3781 catgacgccg gccgaggtgc gcgcgatcat ggcgacacaa gcaagccgcc aggctcgact
     3841 cgctgacgcg gacgacgtca tcgacaacgc cgggccgcgg gaggcgatcg cgccgcaggt
     3901 ggccaagctc gatgcgcgct accgcgagct cgccgcatcg ccaacgtcgc gcgcgatcgc
     3961 cccatgatcc gctacgagca cccgctgaac gagcgcgtcc gcacgctgat gcgcctcgag
     4021 ggcctgtacg cgcgctcgct gtttttcacc ggcaagacga cacccga
//
LOCUS       DATANS010000279         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_98959, whole genome shotgun sequence.
ACCESSION   DATANS010000279 DATANS010000000
VERSION     DATANS010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98959"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VHT22_03990"
     CDS             complement(<1..317)
                     /locus_tag="VHT22_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HEX3136746.1"
                     /translation="MTDAAFLEPDFASVEAAPWRAADADHLGWLESEAIHVLREVAGQ
                     CQHPALLFSGGKDSLVLLRLAEKAFRPGAFPFPLLHIDTGHNFPEVIAFRDRRAAELG
                     ERL"
     gene            complement(314..1036)
                     /locus_tag="VHT22_03995"
     CDS             complement(314..1036)
                     /locus_tag="VHT22_03995"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX3136747.1"
                     /translation="MSSESRIAAVVTLLSRVARDHAPVALASSFGAEDMVLIDLVARH
                     ALPVEILTLDTGRLPDETHMQIDRVREHYGLAIRVLQPDAAALEAFVAANGANAFYRS
                     IELRQVCCAIRKASPLARGLAGKGAWITGQRRAQSITRADLAVEEFDAERGIPKFNPL
                     ADWSEAEVWSYLRSNKVPYHPLHDQGYPSIGCAPCTRPIEPGEDQRAGRWWWEQPEHR
                     ECGLHRSPVKVAVRAIPVEVPA"
     gene            complement(1036..1575)
                     /locus_tag="VHT22_04000"
     CDS             complement(1036..1575)
                     /locus_tag="VHT22_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEX3136748.1"
                     /translation="MPLLTRNRTVIDDRWALLPETATIADVPVSAPAIVPLALWSAQH
                     DALAVRGDIGVWLKPADDPDALAGDVARLPLIAVDFPKFGDGRGYSTARLLREKYRYT
                     GELRAIGDILRDQLYFLLQCGFDTFALQSGRNVAEALAAFDDFSDNYQATVAQPVPLF
                     RRRLHAAAAEAPVSSEGSR"
BASE COUNT          287 a          515 c          540 g          257 t
ORIGIN      
        1 atcaaacgct cgcccagctc ggcggcacgg cggtcgcgaa aggcgatgac ttcggggaag
       61 ttgtgaccgg tgtcgatgtg cagcagcgga aacggaaacg cgcccgggcg gaacgccttt
      121 tcggcgagcc tcaggaggac cagcgaatcc ttgccgcccg aaaacagcag ggcagggtgc
      181 tggcattgcc ctgcgacctc gcgcagcacg tggatcgctt cggattcgag ccagcccaga
      241 tgatcggcat cggcggcgcg ccagggcgcg gcttcaaccg aagcgaagtc cggttcgagg
      301 aatgcagcgt cggtcatgcc ggcacctcga cggggatcgc gcgcaccgcg accttcactg
      361 ggctgcgatg cagcccgcac tcgcggtgct cgggttgctc ccaccaccag cggccggcgc
      421 gctggtcctc gcccggctcg atcggacgcg tgcacggcgc gcagcctatg ctcggatagc
      481 cctgatcgtg cagcggatga tagggaacct tattggatcg caggtagctc cacacctcgg
      541 cttcgctcca atcggccagc ggattgaact tgggtatgcc acgctcggca tcgaattcct
      601 cgacggcaag atcggcgcgc gtgatcgact gcgcgcggcg ctggccggta atccaggcgc
      661 cctttccggc gagtccgcgg gcgaggggac tcgcctttcg gatagcgcaa cagacctgac
      721 gcaactcgat gctgcggtag aaggcgttgg ctccattcgc ggcgacgaat gcttccagcg
      781 cggccgcatc cggctgcagg acgcggatcg ccaggccata gtgttcgcgc acgcgatcga
      841 tctgcatgtg ggtttcgtcc ggcaggcgtc cggtatcgag cgtcaggatt tcgaccggca
      901 gcgcatgccg cgcgacgagg tcgatcagca ccatgtcctc cgcaccgaag ctgctcgcca
      961 gcgctacagg cgcatgatcg cgcgcaaccc gtgacaacag cgtgacgaca gcggcgatgc
     1021 gggattcgct gctcatcatc tcgaaccctc gctcgacacc ggcgcttccg cggccgcggc
     1081 gtgaaggcgg cggcggaaca gcggcaccgg ctgagcgacc gtcgcttggt agttgtcgct
     1141 gaaatcgtcg aaggcggcca gcgcttcggc gacattgcgg ccggactgca gcgcgaaggt
     1201 gtcgaaacca cactgcagaa ggaaatacag ctggtcgcgc aggatgtcgc caatcgcgcg
     1261 cagctcgccg gtgtagcggt atttttcgcg cagcagacgt gcagtcgaat agcctcggcc
     1321 gtcgccgaac ttcgggaagt cgacggcgat caaaggcaga cgagcgacgt cgccggcgag
     1381 cgcatccggg tcgtcggcag gtttcagcca tacgccgata tcgcccctga ctgccagcgc
     1441 atcgtgctgt gccgaccaca gcgcgagtgg aacgatcgcg ggcgcggaaa ccgggacgtc
     1501 ggcgatggtt gccgtttcgg gcagcaacgc ccagcgatca tcgatcacgg tcctgttcct
     1561 agtgagcagc ggcatagacg cgctccttga acggctcga
//
LOCUS       DATANS010000280         1634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_518723, whole genome shotgun sequence.
ACCESSION   DATANS010000280 DATANS010000000
VERSION     DATANS010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518723"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VHT22_04005"
     CDS             complement(<1..927)
                     /locus_tag="VHT22_04005"
                     /inference="COORDINATES: protein motif:HMM:NF023236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor protein PhoR"
                     /protein_id="HEX3136749.1"
                     /translation="MNPVWLSGLAAVALVALAALIAGLSGGVGAACAVVAIGAIVVVG
                     WHLLQLDRLLRWAAGPIDAQVPEGRGAWGTVFTALYRRIRLRSARQRDLRLALERFES
                     GAEAMPEGVVVLDGNNRIEWANSRAERHLGIDARKDRGAPIVNLVRQPAFVHYLTGGE
                     TGEPLVLQSARETALTLSLQIVPFGVEEKLLMSRDITRLEAVARVRRDFIANVSHELK
                     TPLTVLSGFIETLADVPLDEPQRKRCLALMGDQAQSMQRLVDDLLTLSTLESEESPLR
                     ETEFAIVPLLLALSADAKALSAGRHEIALTINH"
     gene            complement(979..>1634)
                     /gene="phoB"
                     /locus_tag="VHT22_04010"
     CDS             complement(979..>1634)
                     /gene="phoB"
                     /locus_tag="VHT22_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEX3136750.1"
                     /translation="QELLRLSLGDAGFDVYAVADAEGAHAEIRRALPDLVLLDWMLPG
                     QSGLALARTLRADARTRELPIIMLTARGDETDRIAGLEAWVDDYVTKPFSPRELRARI
                     KSVLRRRAPEAAQEMLAIGALQLDPAAHRVTAAGTAVDLGPTEFRLLKFLMARPDRVH
                     TRTQLLDQVWGDHVYIEERTIDVHIRRLRLALEPHGQEHMIETVRGTGYRLAAAKRP"
BASE COUNT          259 a          586 c          542 g          247 t
ORIGIN      
        1 atgattgatc gtcaacgcaa tttcgtgcct gcccgcggac aaggccttcg catccgccga
       61 aagcgcaagc agcagcggga cgatcgcgaa ctcggtctcg cgtagcggac tctcctcgct
      121 ttccagcgtc gacagggtca gcaagtcgtc gaccaggcgc tgcatgcttt gcgcctggtc
      181 gcccatcaag gcgagacagc gctttcgctg cggctcgtcc agcggcacgt cggccagcgt
      241 ctcgatgaag cccgacagca ccgtcagcgg cgttttaagc tcgtgcgaga cattggcgat
      301 gaagtcgcgg cgcacgcgcg ccaccgcctc caggcgagtg atgtcgcggc tcatcagcag
      361 cttttcctcg acgccgaacg gaacgatctg cagcgacaag gtcagcgcgg tctcgcgtgc
      421 cgactgcagc actagcggct cgccggtctc gccgccggtc aggtaatgga cgaacgcagg
      481 ctggcgcacg agattgacaa taggcgcgcc gcggtccttc ctggcgtcga tgcccagatg
      541 ccgctcggcg cgcgagttgg cccactcgat gcggttgttg ccgtcgagca cgacgacgcc
      601 ttccggcatc gcttccgcgc cgctttcgaa gcgttcgaga gcgagcctca agtcgcgctg
      661 ccgcgccgag cgcaggcgaa ttcttcggta gagcgcggtg aagacggtgc cccaggcgcc
      721 gcggccttcg gggacctgcg catcgatcgg ccccgctgcc cagcgcagca ggcggtcgag
      781 ctgcaacaga tgccacccga cgaccacgat cgcgccgatg gcgaccaccg cacaggcggc
      841 gcccacgccg ccggatagtc ccgcgatcaa cgccgcgagc gccaccagcg ccactgccgc
      901 gagaccgctc agccacaccg ggttcatcgc cgctcccgcc gcggcaacgc gcgcagcgca
      961 ccgacgtccg cggcccgcct acggacgctt ggccgcggca agccgatatc cggtgccgcg
     1021 cacggtttcg atcatgtgct cctgtccgtg cggctcgagc gcgagccgca gccggcggat
     1081 gtggacatcg atcgtgcgct cctcgatgta cacgtgatca ccccagactt gatcgagcag
     1141 ttgcgtgcgt gtatgcacgc ggtccggccg ggccatcagg aacttgagca ggcgaaactc
     1201 cgtcggcccg agatcgaccg ccgttccggc ggcagtgacg cgatgggcgg cggggtcgag
     1261 ctgcaacgcg ccgatcgcca gcatttcctg cgctgcctcg ggtgcgcgcc ggcgcagcac
     1321 cgacttgatc cgcgcccgca gctcgcgcgg ggaaaacggc ttggtcacgt agtcgtcgac
     1381 ccaggcctcg agtccggcga tcctgtccgt ctcgtccccg cgtgcggtca gcatgatgat
     1441 cggcagctcg cgcgtgcgcg catcggcgcg cagcgtgcgt gccagggcca gtccggactg
     1501 gccgggcagc atccagtcga gcaggaccag gtcgggcagc gcgcggcgga tctccgcgtg
     1561 cgctccctcg gcgtcggcga ccgcgtacac gtcaaagccc gcgtcaccca ggctcagccg
     1621 cagcagctcc tgga
//
LOCUS       DATANS010000281         1885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_309214, whole genome shotgun sequence.
ACCESSION   DATANS010000281 DATANS010000000
VERSION     DATANS010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309214"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="VHT22_04015"
     CDS             <1..1174
                     /locus_tag="VHT22_04015"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX3136751.1"
                     /translation="LWMVGVTGTNGKTSCAHWIAAGLDAAGRRAAVLGTLGNGLIGAL
                     ESGANTTPDAALLQQMLADYRAAGAQAAVMEVSSHGIDQGRVNGVAFDVALFTNLSRD
                     HLDYHTTMAAYGAAKAKLFAWPGLRVGVINADDAFGQSLLDSARGHGHKVLSYGFGAA
                     DVVGSGLTSLPAGGLAFTVETPWGKGRITTPLVGAFNAANLLGVLGVLLVSGVAFEPA
                     LAILAGVEAPPGRMQRLGGGDRPLVVIDYAHTPDALDKVLTALRPAVGEGSELICLFG
                     AGGERDRGKRPEMGRLAARLADRVLVTSDNPRGEDPAAIASEIVHGIRDTGNRRYSVE
                     LDRAIAIASAIGEAGRGDIVLLAGKGHETYQESNGARLPFSDAEHAARALMSWSGK"
     gene            1171..>1885
                     /locus_tag="VHT22_04020"
     CDS             1171..>1885
                     /locus_tag="VHT22_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX3136752.1"
                     /translation="MIDAATVAQAISARLEGGNAWFDGVSIDSRVVRPGELFVAIKGE
                     RFDGHEFVAQAFSRGAAAAIVAADHAAGIAASAAGGGRSLLAVDDPLQSLASLAAFWR
                     RRFTLPVVAIVGSNGKTTVKEMTAAILRAANGTDRVLATAGNLNNHIGLPLMVLRLRA
                     MHLVAVFEIGMNHVGETAQLGEIAQPTIAVLNNAQREHQEFMKSVADVAAEHATILGT
                     LQQRGIAVINADDDYAPFWN"
BASE COUNT          303 a          616 c          668 g          298 t
ORIGIN      
        1 cctgtggatg gtcggcgtca ccggcaccaa cggcaagaca tcgtgcgcgc actggatcgc
       61 cgcgggccta gatgccgcgg gacggcgcgc cgcggtgctc ggcacgctgg gcaatggatt
      121 gatcggtgcg ctcgagtcgg gggcgaacac gacgcccgat gcggcgctgc tgcagcagat
      181 gctcgccgat tatcgtgcgg cgggcgcgca agccgcggtg atggaagtct cctcgcatgg
      241 catcgaccag ggacgggtca acggcgtcgc gttcgacgtc gccttgttca ccaatctgtc
      301 gcgcgatcat ctcgactatc acaccacgat ggcggcgtat ggcgccgcga aagcgaagct
      361 gttcgcatgg ccggggctgc gcgtcggcgt gatcaatgcc gacgacgcgt tcgggcagag
      421 cctcctcgac agcgctcgcg gccacggcca caaggtgctg agctacggtt tcggcgccgc
      481 cgacgtcgtc ggcagcggac tcacatcgct gccggcggga ggcctggcat tcaccgtcga
      541 gacgccgtgg ggcaagggcc ggatcacgac gccgctggtc ggtgcattca atgcggcgaa
      601 tctgctcggc gttctcggcg ttctcctggt cagcggcgtc gcgttcgaac cggcgctcgc
      661 catcctcgcc ggcgtcgagg cgccgccggg acgaatgcag cgcctcggcg gcggcgaccg
      721 gcccctcgtg gtgatcgatt acgcgcatac gccggacgcg ctggacaagg tcttgacggc
      781 gctgcgtccc gcggtcggcg aaggcagcga gctcatctgc ctgtttggtg ccggcggcga
      841 gcgcgatcgc ggcaagcgcc ccgaaatggg ccggcttgcg gccaggcttg ccgatcgcgt
      901 gctggtcacc agcgacaacc cgcgcggcga agaccccgcc gcgatcgcca gcgaaatcgt
      961 gcacggcatt cgcgacacgg gcaatcggcg ctattcggtc gagctcgacc gcgcgattgc
     1021 catcgccagc gcgatcggtg aggccggacg cggggacatc gtgctgctgg ccggcaaggg
     1081 tcacgaaacc tatcaggaga gcaacggcgc gcggctgcca ttcagcgatg ccgagcatgc
     1141 ggcgcgcgcg ctgatgtcgt ggagcggcaa atgatcgatg cggcgaccgt ggcgcaggcg
     1201 atatccgccc ggctcgaggg gggcaatgcc tggttcgacg gcgtcagcat cgattcgcgc
     1261 gtcgttcgtc cgggcgagct ctttgtcgcg atcaaaggcg agcgcttcga cggacacgaa
     1321 tttgtcgcgc aggcattttc tcgcggcgcc gccgccgcca tcgtggcggc cgatcacgca
     1381 gcggggatcg ccgcgtcggc tgcaggcggc gggaggagtc tcctcgcagt cgacgaccca
     1441 ctgcaatcgc tggcgagcct cgccgcgttc tggcgccggc gctttacgtt gccggtcgtc
     1501 gccattgtcg gcagcaacgg caagacgacg gtcaaggaaa tgacggcggc gatcctgcgc
     1561 gccgcgaacg gcaccgatcg ggtgctggcg actgcgggca atctgaacaa tcacatcggc
     1621 ctgccgctga tggtgttgcg tttgcgcgcc atgcatctgg tcgccgtgtt cgaaatcggc
     1681 atgaatcacg tcggcgagac cgcgcagctc ggcgagattg cgcagccgac gatcgccgtg
     1741 ctcaacaacg cgcagcgcga gcaccaggag ttcatgaaaa gcgtcgccga cgtcgcggct
     1801 gagcacgcga cgatcctcgg cacgctgcag cagcgaggga tcgccgtcat caatgctgac
     1861 gacgactacg ctccgttctg gaacg
//
LOCUS       DATANS010000282         2892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_645736, whole genome shotgun sequence.
ACCESSION   DATANS010000282 DATANS010000000
VERSION     DATANS010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645736"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="VHT22_04025"
     CDS             complement(<1..1159)
                     /locus_tag="VHT22_04025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEX3136753.1"
                     /translation="MTKGRRTRAVALPGSAKAPFAEAKYVGPARKSDRVEVTVRLRRK
                     KALRAPAGGAAVRRIGYETFRAEHGAAPRDVAVVEKFAHAHGLDVTRVSLAQRAVDLS
                     GTVAAMEVAFGTRLSCYRHGEREYRVRTGAIMIPRELAAIVEGVFGLDNRPHVEPHFR
                     IAKTGGKNKPRAAASSFTPLEVAALYAFPKGYDGKGQTIAILEFGGGYRAADLTKYFS
                     RLGVPMPSVTAVSVGGAHNAPTGNPNSPDGEVVLDIEVAGAVAPGAQIVVYFAPNTDD
                     GFINALYAAIHDNLRRPGIVSISWGSAETNSTLQSLKDYDAACIDAAALGITVCAAAG
                     DHGSSDTDPPGKRAMVDFPASSPHVLACGGTRLQASGAAIVAETVWNTHDGW"
     gene            1253..1675
                     /locus_tag="VHT22_04030"
     CDS             1253..1675
                     /locus_tag="VHT22_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136754.1"
                     /translation="MRKIPLFAAALGLAVGAAALPAKAMICNIVFDRNDQVIYRDVTP
                     PLDLSDGGSRARAAMRQRGEYLMVIDTDRCAQVVPRTDTSNAASVEDIVAGMRPYMGR
                     GGGQPTSGGAAAGGTGSTVRAAAPAAASTSRGSSTRAY"
     gene            complement(1747..1992)
                     /locus_tag="VHT22_04035"
     CDS             complement(1747..1992)
                     /locus_tag="VHT22_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX3136755.1"
                     /translation="MAKAKAKSRKTGAGESVYRITELVGTSQVSWEDAARRAVETAAG
                     SLRDLRIADIIKLDMRVENGKVAAYRARVALSFKYEG"
     gene            complement(2106..>2892)
                     /locus_tag="VHT22_04040"
     CDS             complement(2106..>2892)
                     /locus_tag="VHT22_04040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3136756.1"
                     /translation="VGFALLVRWERRAVDPVLPLRLLGTPAIARSDAVVVCFGATLFS
                     TILFLPLYLQLGRGFAIGQSGLLLLPITLAMVVSSAITGKLVTRTGRVTIFPQLGMSI
                     ATLAFIALATAVGTAPTFAIMGLTTVVGVGIGMVMPPTQVTVQLAAGPKALGAATASI
                     ALSRAIGGAAGVALIGAVLIGALGVAGGSLPALLARVLDAGPDAVAQMSQAERATMGA
                     RFDHAYRIVFGLLAGVTLVGALLARTIPRPDWSTPVPWSKHGE"
BASE COUNT          461 a         1003 c          952 g          476 t
ORIGIN      
        1 cccacccgtc gtgcgtgttc cacacggtct ctgcgacgat cgccgccccg ctggcctgga
       61 gccgcgttcc gccgcaggcg agtacgtgcg gagacgaggc cggaaaatcg accatcgcgc
      121 gcttccccgg cgggtcggta tcgctcgatc catggtcgcc cgccgcagcg cagacggtaa
      181 tgcccagcgc cgcggcatcg atgcacgcgg cgtcgtagtc cttcagcgat tgcagggtag
      241 agttggtctc ggcgctgccc cagctgatcg acacgatccc cggtcggcgc aggttgtcgt
      301 gtatcgccgc gtacagcgcg ttgatgaatc cgtcgtcggt gttcggcgcg aagtagacga
      361 cgatctgggc tcccggcgcg acggcgccgg ctacctcgat gtcgagcacg acctcaccgt
      421 cgggactgtt cggattgccg gtcggagcgt tgtgcgcgcc gccgaccgac accgcggtga
      481 ccgacggcat cgggacgccg agccgagaga aatatttggt cagatcggcc gcccggtacc
      541 cgccaccgaa ttccaggatt gcgatcgtct ggcccttgcc gtcgtagcct ttgggaaacg
      601 catacaaggc ggcgacctcg agcggagtga agctggaggc ggccgcgcgc ggcttgttct
      661 tgccgccggt cttcgcgatg cggaagtgcg gctcgacatg cggtcggttg tcgaggccga
      721 acacgccttc gacaattgca gcgagctcgc gaggaatcat gatggcgccg gtccgcaccc
      781 ggtactcacg ctcgccatgc cggtagcaac tgaggcgggt gccgaacgct acctccatcg
      841 cggcgactgt tccggacaga tcgactgcgc gctgcgcgag gctgacgcgc gtgacatcca
      901 gaccgtgcgc atgcgcgaat ttttccacga cggcgacatc gcgcggcgcg gcgccgtgct
      961 cggcgcgaaa ggtttcgtag ccgatgcgac ggaccgcggc gccgccggcc ggcgcgcgca
     1021 gcgctttctt gcggcgcagc cgcaccgtca cctcgacccg gtcggacttc cgtgccgggc
     1081 cgacgtattt cgcctccgcg aacggcgcct tggcgcttcc cggcaatgcc acggctcgag
     1141 tcctgcgtcc tttggtcatg ccattctcct gatctcgtcc cgcccggatg aaggttgcgc
     1201 cgcgtctgcc gcaggcatac gatgcaagcc tcgagctacc ttaaggatta ccgtgcgcaa
     1261 gattccgctt tttgcggctg ctctcggcct cgccgtcggc gcggccgctc ttcccgccaa
     1321 ggccatgatc tgcaacatcg tgttcgatcg caacgatcag gtaatctatc gcgacgtcac
     1381 gccgccgctc gatctctccg acggcggatc cagagcgagg gcggcgatgc gtcagcgcgg
     1441 tgaatatctg atggtgatcg acaccgaccg ttgcgcgcag gtcgtgccaa ggacagacac
     1501 ctccaacgcg gccagcgtcg aagacatcgt cgcgggaatg cggccgtaca tggggcgcgg
     1561 cgggggccag cctacatccg gcggcgcggc ggccggtggg accggcagta ccgtccgcgc
     1621 agcggcgcct gccgcagcga gcacgtcgcg cggcagctcg actagagcgt attagcggca
     1681 ctcgttcgcc gcgcggcgaa cgctgtactg ccaggcttgc gcggcggctt tcgccgccat
     1741 cagcctctaa ccctcgtact tgaacgacag cgcgacacgg gcgcggtacg ccgcgacctt
     1801 gccgttttcg actctcatat ccagtttgat gatgtcggcg atacgcagat cgcgcagcga
     1861 tcccgccgcg gtttcgacgg cgcgcctcgc cgcgtcctcc cacgaaacct gactggtgcc
     1921 gaccagctcg gtgatccggt agacgctctc gcccgcgccg gttttcctgc tcttcgcctt
     1981 tgcctttgcc atgcgttcct cctggtcgac cgtgttgatc gccattcttt ttattccgag
     2041 gcaccggcat tgtcaatgcg caggcgcagc acatcgatcg gcaccaggtc ggtcgacggg
     2101 ggcggtcatt cgccgtgctt gctccatggc accggcgtcg accagtccgg gcggggaatg
     2161 gtgcgcgcca gcaacgcgcc gaccagcgtc accccggcca gcaggccgaa gacgatgcga
     2221 taggcgtgat cgaaccgggc tcccatcgtt gcgcgctcgg cctgcgacat ctgtgcgacc
     2281 gcgtccggtc ccgcgtcgag cacgcgcgcc agcagcgccg gcagcgaacc gccggcaacg
     2341 cccagcgcgc cgatcagcac tgccccgatc agcgcgacac cggccgcgcc gccgatcgca
     2401 cgcgacagcg cgatcgatgc ggtcgcagcg ccgagcgcct tcggtccggc cgccagttgc
     2461 acggtgacct gcgtcggcgg catgaccatg cctatcccga cgcccacgac ggtggtcaat
     2521 cccatgatcg cgaacgtggg cgcggtgccg accgctgtcg cgagagcgat gaatgccagc
     2581 gtggcgatgc tcattccgag ctggggaaat atcgtcactc ggccggtgcg cgtgaccagt
     2641 ttgccggtga tggccgacga aacgaccatc gccagggtga tcggcagcag cagcagaccc
     2701 gattgtccga tcgcaaagcc gcgacctagc tgcaggtaca gcggcaggaa caggatcgtc
     2761 gagaacagcg tcgcaccgaa gcagacgacg accgcgtcgg agcgggcgat cgcgggcgtt
     2821 cccagcaggc gcagcggcag gaccggatcg acggcgcggc gctcccaccg caccagcagc
     2881 gcgaatccca ca
//
LOCUS       DATANS010000283         1950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_810697, whole genome shotgun sequence.
ACCESSION   DATANS010000283 DATANS010000000
VERSION     DATANS010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810697"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="VHT22_04045"
     CDS             <1..1449
                     /locus_tag="VHT22_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX3136757.1"
                     /translation="GGGGAGLAAASEAARFGRKVVLLEKNERLGGSTAWSVGSISATG
                     TPHQRARGIQDSPQEHFEDLEVLAGRYANRDNRALRRILVDHTTEMLDWLMSLGVVFV
                     GPMPEPPHRYNRMHNVVPNSKSFAYHLTKHCRALGVDIRLNTATQRLITENGRVVGVE
                     ARLPDGTTHEFRAKGGVILAAGDYSNGRDMKARFASPDVVEVDAVNPTATGDGHRMAL
                     ELGATVINGDIVRGPIMRFIPPTRPNFIQKLPPMKPIAKMIAWAMDHVPQFLLRPFLM
                     SFLTTALGPSPDLYKEGAVLINKNGQRFTDEIGKPNLFTAKQPDRIAWIVFDHKLAQK
                     FSGWPYFVSTAPGVAYAYLADYRRNRADIYHQSDTIEGLAASMGVAPESLKRSLTDYN
                     ATRETRPAIDQGPFYALGPVKSYVVFTDGGLKVSTKHEVQREDGSVIPGLYAAGSTGQ
                     GGLLLEGHGHHLGWAFISGRLAGRNAAENVHV"
     gene            1446..>1950
                     /locus_tag="VHT22_04050"
     CDS             1446..>1950
                     /locus_tag="VHT22_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136758.1"
                     /translation="MIFNSQSGITDNSRAADWDVWYLEHLRIMATVPGVSSAQRFKTD
                     TPGASPSLAIYSVTSADVFKDPYYLSVRGMGEWLALIDRRYYKRNLFSGLDYAPQVDD
                     SQCMLVADRDFLEGAIGGIEFTWLKCEGIDRSTPYRGLAVIDADKVPAFDATIAVYRP
                     ASPRMTPS"
BASE COUNT          362 a          649 c          600 g          339 t
ORIGIN      
        1 ggcggcggcg gcgccgggct cgccgccgcc agcgaggcgg cgcgcttcgg ccgcaaggtc
       61 gtgctgctcg agaagaacga gcggctcggc ggcagcaccg cctggtcggt cggctcgatc
      121 tcggcgaccg gcacgccgca ccagcgcgct cgcggcatcc aggattcgcc gcaggagcat
      181 ttcgaggacc tcgaggtgct ggccggccgc tacgccaacc gcgacaaccg cgcgctgcgc
      241 cgcatcctgg tcgaccacac gaccgagatg ctcgactggc tgatgtcgct gggtgtcgtg
      301 tttgtgggcc cgatgcccga acctccgcat cgctataacc ggatgcacaa cgtggtgccg
      361 aattcgaaat cgtttgccta ccacctgacg aaacactgcc gcgcgctcgg cgtggatatc
      421 cggctcaata cggcgacgca gcgcctgatc acggagaatg gccgcgtcgt gggcgtcgaa
      481 gcgcgtctgc ccgatggcac gacgcatgaa tttcgcgcca aaggcggcgt gatactcgcc
      541 gccggcgatt acagcaacgg gcgcgacatg aaagcgcgct ttgcttcgcc ggacgtcgtc
      601 gaagtcgatg cggtgaatcc aaccgcgacc ggcgacggcc accgcatggc gctcgagctc
      661 ggcgcgacgg tcatcaatgg cgacatcgtg cgcggtccca tcatgcgctt cataccgccc
      721 acccggccca acttcattca gaagctgccg ccgatgaaac ccatcgccaa gatgatcgcg
      781 tgggcgatgg accacgtacc gcaatttctg ctgcggccgt tcctgatgag cttcctgacc
      841 accgcgctgg gtccttcgcc cgatctctac aaggaaggcg cggtccttat caacaagaac
      901 ggccagcgct ttaccgacga gatcggcaag cccaacctct ttacggcgaa gcagcccgac
      961 cgcatcgcgt ggatcgtctt cgatcacaag ctcgcgcaga aattctcggg ctggccgtat
     1021 ttcgtgtcga ctgcgcccgg tgtcgcctac gcgtacctcg cggattaccg gcgcaatcgc
     1081 gccgacatct atcaccagtc cgacacgatc gaaggattgg ccgcgagcat gggcgttgcg
     1141 ccggaaagtc tcaagcgctc gctgactgat tacaacgcga cgcgcgaaac gcggcccgca
     1201 attgatcagg ggccttttta cgcgctcggg ccggtcaaaa gctatgtggt gttcaccgac
     1261 ggcgggctca aagtctcgac caagcacgaa gttcagcgcg aggacggcag cgtgattccc
     1321 ggcttgtacg cggcgggctc gacggggcag ggcggcctgc tgctcgaagg ccacggccat
     1381 cacctcggct gggcgtttat ctccggccgg ctcgccggcc gcaacgcggc cgaaaacgtg
     1441 cacgtatgat ctttaacagt caaagcggca tcaccgacaa cagtcgcgca gccgattggg
     1501 atgtgtggta tctcgaacac ctacgcatta tggcgacggt gccgggcgtt tcgtccgcgc
     1561 agcgtttcaa gaccgataca cccggcgctt cgccgtcgct cgccatctac agcgtcacct
     1621 ccgccgacgt gttcaaagac ccgtattacc tgagcgtgcg cgggatgggc gagtggctgg
     1681 ctctcatcga ccgccgctat tacaagcgca atctcttctc cggactcgac tacgcgccgc
     1741 aggttgacga ttcccagtgc atgctcgtcg ccgaccgcga ttttctagag ggcgcgatcg
     1801 gcggcatcga gttcacatgg ctgaaatgcg aaggcatcga ccgctcgacg ccgtaccgcg
     1861 gactcgcggt gatcgacgcc gacaaagtgc cggcattcga tgcgacgatt gcagtgtatc
     1921 gacccgcttc gccacgaatg acgccatcca
//
LOCUS       DATANS010000284         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_183970, whole genome shotgun sequence.
ACCESSION   DATANS010000284 DATANS010000000
VERSION     DATANS010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183970"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VHT22_04055"
     CDS             <1..362
                     /locus_tag="VHT22_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136759.1"
                     /translation="EETAWTFHPEALVGIYFWEQPDQPRSFLRAAFCGRVSTHDPARR
                     LDRGIERAVWMTREQIVMRQSRLRSPMVLGCIDDYLDGTRYPLDVITHVLKDPTKLST
                     ADLRSRSFDHPALNRVN"
     gene            394..1500
                     /gene="mnmA"
                     /locus_tag="VHT22_04060"
     CDS             394..1500
                     /gene="mnmA"
                     /locus_tag="VHT22_04060"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX3136760.1"
                     /translation="MSATPVIVGMSGGVDSSVAAWLLQQQGYDVQGLFMSNWDEDEDG
                     YCTAAEDFQDARSVCEQLRIPLHKVSFAGDYRERVFAHFLEEYRAGRTPNPDVLCNRE
                     IKFGVCFDYARRLGAEWVATGHYARVEHGTDGTSPRLLRGVDAGKDQSYFLHAMPSQA
                     LARTLFPIGSLQKSEVRRMARELTLPVFDKKDSTGICFIGERPFAEFLAQYLPAQPGD
                     IETAEGRKVGEHRGLMYYTLGQRQGLRIGGRHGASEDAWYVAGKDLKRTVLVVAQRHD
                     HPSLCSGGRTALQLTWVAGTAPAAHFRCTAKVRYRQPDQNCEVRVLADARCTVQFDEP
                     QRAVTPGQYVVFYRGEECLGGGVIEASQGQPQPL"
BASE COUNT          257 a          522 c          508 g          257 t
ORIGIN      
        1 tggaagagac ggcttggacg ttccacccgg aggcgctcgt cggcatctac ttctgggagc
       61 agcccgacca gccccgcagc ttcctgcgtg cggcgttctg cggtcgggtc agcacgcacg
      121 atccggcgcg tcgcctcgat cgcggcatcg agcgcgctgt gtggatgacg cgcgaacaga
      181 tcgtgatgcg ccaatcgcgg ctgcgcagcc cgatggtgct cggctgcatc gacgactacc
      241 tggacggcac ccgctatccg ctggacgtca tcacacacgt cctcaaggac ccgaccaagc
      301 tgagcacggc cgacctgcgc tcgcgctcct tcgatcatcc cgcgctcaac cgcgtgaact
      361 agtcttcgag aagcggatac catccgctcc cccatgtctg ccactcctgt aatcgtcggt
      421 atgtccggcg gcgtcgactc gtcggtcgcc gcctggctgc tgcagcagca gggctacgac
      481 gtgcagggac tcttcatgag caactgggac gaagacgagg acggctactg cacggccgct
      541 gaggatttcc aggacgcgcg cagcgtctgc gagcaactgc gcattccgct gcacaaggtg
      601 agcttcgcgg gcgactaccg cgagcgcgtc ttcgcccatt tcctcgagga atatcgcgcc
      661 ggccgcacgc cgaatcccga cgtgctgtgc aatcgcgaga tcaagttcgg cgtgtgcttc
      721 gactacgcgc gacggctcgg cgccgagtgg gttgccacgg gtcactatgc gcgggtcgag
      781 cacggcaccg atggcacgag tccgcgactg ctccgcggcg tcgatgccgg caaggaccag
      841 agctacttcc tgcacgcgat gccttcgcaa gccctcgcac gcacgctgtt cccgatcggc
      901 tcgctgcaga agagcgaagt gcggcgcatg gcacgcgagc tcacgctgcc ggtgttcgac
      961 aagaaggaca gcaccggcat ctgctttatc ggcgaacggc cgttcgccga gttcctggcg
     1021 cagtacctgc ccgcgcagcc cggcgacatc gagacggccg aaggccgcaa ggtcggcgag
     1081 catcgcggcc tcatgtacta cacgctcggc cagcgccagg ggctgcgcat cggcgggcgc
     1141 cacggggcgt cggaggatgc ctggtatgtg gccggcaagg atctgaagcg cactgtgctc
     1201 gtggtcgcgc agcgccacga tcatccgtcg ctgtgcagtg gcggccggac cgcgttgcaa
     1261 ctgacttggg tcgcagggac agcgccagcg gcgcatttcc gctgcacggc caaggtccgg
     1321 taccgccagc cggatcagaa ctgcgaggtc cgcgtgctcg cggacgcacg ttgcacggtc
     1381 caattcgacg agccgcagcg cgcagtcacg cccgggcagt acgtcgtgtt ctatcgcggc
     1441 gaggaatgcc tgggcggcgg cgtcattgag gcgtctcagg ggcagcccca gccgctataa
     1501 tccgccactt ttggcgggcg cagtccgctc agccatatct ttag
//
LOCUS       DATANS010000285         5103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_141263, whole genome shotgun sequence.
ACCESSION   DATANS010000285 DATANS010000000
VERSION     DATANS010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141263"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..936)
                     /locus_tag="VHT22_04065"
     CDS             complement(19..936)
                     /locus_tag="VHT22_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3136761.1"
                     /translation="MTGRRIGPLFCILLAGYATAAAAQSAAPASTPVPARTINTLQRI
                     RDTGTLPIGIRDASFPFSFVNHGKGPQGYTIDICLEIADAIKNEMAMPRLEVRYIPVS
                     PANRVTALQDGKIDLECGSTSNTRELQKEVTFAYTTFVAGIRLLARKSSQISGIDDLR
                     GKTIVVTRGTVGEKLMRDINAERLLKMNIVVAADHDESFRAVDQGGAVAFPMDDVLLY
                     SLIAKAKRPDDFAVVGKYLSVEPYAIMMRKGEAQFETIVDRTLSQLFASGEIRRLYDK
                     WFATRELRVPLNPLLKEAFAMPNTYPAWP"
     gene            complement(947..1720)
                     /locus_tag="VHT22_04070"
     CDS             complement(947..1720)
                     /locus_tag="VHT22_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136762.1"
                     /translation="MAKKWTRFPHPDKAFAYEGAALKKNWDRLHRGDAEPYPQAEAAQ
                     RAWRHYHAGEFQQAVETGQAAGGPGINAAIKAQAIYANYLETDKSRLALFEEAAAWAD
                     ARRAEAPKDANAHYLYAYALGRYGQGISVAKALAQGMGGKIKDALGKAMKLAPQHADA
                     HTAFGAYQAEVIAKVGALVAGMTYGAKRDSALEHFQKAMKLNPDSAIARIEHANGLIL
                     LFGNERLTDATKLYEQAAACKPADAMERLDVELARSELE"
     gene            complement(1816..2457)
                     /locus_tag="VHT22_04075"
     CDS             complement(1816..2457)
                     /locus_tag="VHT22_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3136763.1"
                     /translation="MAERKRPRRTRARIVETSLQLFNRDGAPNVTTADISDEMGISPG
                     NLYYHFRNKDQIIAELFAAFEQRLDGLFAAPAGRLADVEDLWLVLHLLFEAMWDYRFI
                     YRDLDEMLSRNPRLESRFSRLVGRGRRTTQKLCADMVAAGAMAASESEIAALADNVAL
                     VATYWMSYQKIASADRRQPPSLDRAAYQVLSLIAPFLRGPSRTLLARLSQGYL"
     gene            complement(2482..3750)
                     /locus_tag="VHT22_04080"
     CDS             complement(2482..3750)
                     /locus_tag="VHT22_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX3136764.1"
                     /translation="MSGAATPQRRRRGGKNRARRPRIGLALAGGGPLGGIYEIGALLA
                     LSESLDGLDFDDLDVYVGVSSGGFIAAGLANGISPPQMYRLFIEDGAAAGLSPSLFLR
                     PALREFLRRALSVPRLLAKATAVYLRHPWRGSALQAYAALGRAIPTGVFDQRAIDAFL
                     RRLFTTGNRTDDFRQLRRQLFLVATDLDTGSAVTFGGPAADHVPISKALQASAALPGL
                     FPPVRIHGRHYVDGALNKTLHASVALDRGVRLLLCINPLVPFDASAASRNGRPSVEKL
                     DHGGLPLVLAQTFRAIIHSRMRVGMEKYRQQYPDADVLLFEPDREDADMFFANIFSYA
                     HREQLCEAAYRSTRRNLLARQALLAPLLAKHGVRLNLERLDDGRRSIADALNDPRPLQ
                     ARPRGVRQATRDLAHTLDHLEAWLAAAPAA"
     gene            complement(3813..4268)
                     /locus_tag="VHT22_04085"
     CDS             complement(3813..4268)
                     /locus_tag="VHT22_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX3136765.1"
                     /translation="MSKQVSFNRSVVSANALAESALFASRQAWLAGLGAASISREWAR
                     HEAGNTFRALVRKGSTVEKNAIQVIGGRVESSVAIATSLWRVARATALATANRLAETA
                     ASALPRVRIPAFAASAAAPKPRKHAKTRTIKARTHRSVRSVKRTARKSK"
     gene            complement(4348..>5103)
                     /locus_tag="VHT22_04090"
     CDS             complement(4348..>5103)
                     /locus_tag="VHT22_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX3136766.1"
                     /translation="IVGKRTFLHPTVVSAALMPHRVDGYAGAPQTVYSDHFLDSLPVD
                     GPIGYKLEAPPLHPVLVATTLSGDGASHARWMRELPRMQVLIALMRDGFHAQSEGGTV
                     SLQGDGTPSLDYPLNDYFWDGARRALATMAEIQFAAGATTVMPVHAAGASYADWSEAQ
                     SGVAGLPLKPLATAVVSAHVMGGCPLGADSRRAVTDHDGRHHHLDNLYVFDGSLFPTS
                     VGANPQLSIYGIVAKLASALAQRLAPTRTALRD"
BASE COUNT          868 a         1631 c         1678 g          926 t
ORIGIN      
        1 cgagctggat cgcgccgcct atggccatgc cgggtaagtg ttcggcatgg cgaaagcctc
       61 cttgagcagc gggttcaatg gcaccctcag ctcgcgggtg gcgaaccatt tgtcgtacag
      121 gcgccggatc tcgccgctgg caaatagctg cgataacgtc ctgtcgacga tcgtttcgaa
      181 ttgcgcttcc cctttgcgca tcatgatcgc gtagggctcg accgacagat acttgccgac
      241 gacggcgaaa tcgtccggcc gcttcgcctt cgcaatcagg ctgtacaaca gcacatcgtc
      301 catcgggaaa gcgaccgcgc cgccttgatc gaccgctctg aacgactcgt cgtgatcggc
      361 ggcgacgacg atgttcatct tcagcagccg ttcggcgttg atgtcccgca tcagcttttc
      421 gccgaccgtg ccccgcgtga cgacgatcgt cttcccgcgc aggtcgtcga tgccggaaat
      481 ctgcgatgac ttccgggcca aaagcctgat tcccgcgacg aaggtcgtat aagcgaaggt
      541 gacctctttc tgcagctcgc gcgtattgct ggtcgacccg cattccaggt cgatcttgcc
      601 gtcctgcagc gcggtgacgc ggttggcggg cgacaccggg atgtatctga cctcgagtcg
      661 cggcatggcc atctcattct tgatagcgtc cgcgatctcg aggcagatgt cgatcgtata
      721 gccctgcggc cccttgccgt gattgacgaa cgagaaggga aacgaggcgt cgcgaatgcc
      781 gatcggcagg gtgccggtat cgcgaatacg ctgcagcgtg ttgatcgtgc gtgccggcac
      841 tggagttgac gcgggcgccg cgctctgcgc cgcggcagcg gtcgcgtagc cggcaagcag
      901 gatgcagaac agagggccga tgcgccggcc ggtcatcgaa ctgcggtcat tccagctcgg
      961 agcgcgcgag ctcgacatcc aggcgctcca tcgcatcggc gggtttgcac gccgccgcct
     1021 gttcataaag cttggtcgca tcggtaagac gctcgttgcc gaagagcagt atcagtccgt
     1081 tcgcgtgctc gattctcgca atcgccgaat cggggttgag cttcatcgcc ttctggaaat
     1141 gctcgagtgc tgagtctctt tttgcgccat aagtcatgcc ggccaccagc gctccgactt
     1201 tggcgatgac ttcggcctga taagcaccga acgcggtatg cgcatcggca tgctggggcg
     1261 cgagcttcat cgcctttccg agcgcatctt tgatcttgcc gcccatgccc tgggccagcg
     1321 ccttcgcgac cgagatgcct tggccgtagc gcccgagcgc gtaggcgtac aggtagtggg
     1381 cgttggcgtc cttgggggcc tcggcgcggc gcgcatcggc ccacgctgcc gcttcctcga
     1441 acaatgccag ccgcgacttg tcggtttcca gatagttggc gtaaatcgct tgcgccttga
     1501 tcgccgcatt gataccgggt ccgccggcag cctgtccggt ttcgaccgcc tgctggaatt
     1561 cgccggcgtg gtaatgccgc cacgcgcgct gcgcagcttc cgcctgcgga tacggctcgg
     1621 catcgccccg gtgcaatcga tcccagtttt ttttcagcgc ggcgccctca taggcgaagg
     1681 ccttgtccgg gtgcggaaag cgtgtccatt ttttcgccat tcgacctcct gctcggggtt
     1741 tcagcgagac gacggcgctc gattatcgct cgcgcgcctc gtcgccgcgt gctgcgttgc
     1801 gtacgatatg cgtgatcaca agtacccctg actcaatcgc gccagcagcg tccgcgaagg
     1861 gccgcgcaga aacggcgcga tcagcgacag aacctggtag gcggcacgat cgagactggg
     1921 cggctgccgc cggtccgcgg acgcgatctt ctgatacgac atccaatagg tcgcaaccaa
     1981 cgccacgttg tccgcaagcg ccgcaatttc cgattcggag gccgccatcg cgcctgccgc
     2041 gaccatgtca gcgcataact tctgtgtggt tcgtctgccg cggccgacaa ggcgggagaa
     2101 gcgagactcg agcctcgggt tgcgcgacag catttcatcc aggtcgcgat agatgaatcg
     2161 ataatcccac atcgcctcga acagcaggtg caggaccagc cacaggtcct cgacgtcggc
     2221 cagccggccg gcgggcgccg cgaacaggcc atccagccgc tgctcgaacg cggcgaacaa
     2281 ctcggcgatg atctgatcct tgttgcggaa atgatagtac aggttgccgg gactgatgcc
     2341 catctcgtcg gagatgtccg ccgtcgtgac gtttggcgcg ccgtcgcgat tgaacagctg
     2401 caggctggtc tcgacaatgc gcgcgcgcgt tcggcgcgga cgtttgcgtt ccgccatcgt
     2461 tttcgttccg gccgccaggc gtcaagccgc gggcgccgcg gcgagccagg cctccaggtg
     2521 atccagcgta tgcgcgagat cacgcgttgc ctgccgcacg ccgcgcgggc gcgcctgcag
     2581 cggtcgcgga tcgttgagcg cgtcggcgat cgaccgacgc ccatcgtcga gacgctcgag
     2641 gttcaagcgg acaccgtgct ttgccagcag aggcgccagc agcgcctgcc gggcaagcag
     2701 attgcgccgg gtgctgcgat acgccgcctc gcacagctgt tcgcgatggg catagctgaa
     2761 gatgttggcg aaaaacatgt cggcgtcctc gcgatcgggc tcgaacagca gcacgtcggc
     2821 atccggatac tgctgccggt acttttccat gccgaccctc atgcgtgaat gaatgatcgc
     2881 gcgaaacgtc tgggcaagca ccaatggcag cccgccgtga tcgagctttt cgaccgaagg
     2941 acggccgttg cgcgatgccg cgcttgcgtc gaacgggacc agcgggttga tgcacagcag
     3001 cagtcgcact ccgcgatcga gcgcaaccga ggcgtgcagc gtcttgttca atgcgccgtc
     3061 gacgtagtgc cgcccgtgga tgcggaccgg cgggaataat ccaggcaatg ccgccgacgc
     3121 ctggagcgcc ttgctgatcg gcacgtgatc agcggccggg ccgccgaaag tcaccgcgct
     3181 gccggtatcc aggtccgttg ccaccagaaa cagctggcgg cggagctgcc ggaaatcgtc
     3241 agtgcggtta ccggtcgtga acagccgcct caggaaagcg tcgatcgcgc gctgatcgaa
     3301 cacgccggta gggatcgcgc ggcccagcgc ggcgtacgcc tgcagcgcgc tgccgcgcca
     3361 tggatggcgc aggtagacag cggtggcctt ggccagcagc cgcggaaccg acagcgcccg
     3421 gcgcaagaat tcacgcagtg cggggcgcag gaaaagcgac ggcgagagtc cggcggcggc
     3481 gccgtcttcg atgaacagcc tgtacatctg gggcggtgaa atgccgttcg cgagccccgc
     3541 tgcgataaag ccgcccgatg aaacgccgac ataaacgtcg agatcgtcga agtccaggcc
     3601 gtcgagcgat tccgacagcg cgagcagtgc gccgatttcg tagatgccac cgagcgggcc
     3661 gccgccggcc agcgcgaggc cgatgcgcgg gcgtcgagcc cgattttttc cgccgcggcg
     3721 ccgccgctgc ggcgtcgccg ctccggacat cgatctgtct ccgttgggct ctttcgcgca
     3781 accgtcgcgc ggtcccgccg gattctacta cgctacttcg acttcctggc ggtgcgtttg
     3841 acgctgcgga cgctccgatg ggtacgtgct ttgatcgtgc gggtcttcgc gtgtttgcgc
     3901 ggcttcggcg ctgcggcgct tgctgcgaac gccgggatcc tgacgcgggg gagcgccgag
     3961 gcggcggttt cagccaggcg gtttgccgtg gccagcgcgg ttgcgcgtgc gacgcgccaa
     4021 agcgaagtcg cgatcgcaac cgaggattcg acgcggccgc cgatgacttg aatcgcgttt
     4081 ttctcgacgg tcgagccctt gcgaaccagc gcgcggaacg tgttgccggc ttcgtgacgt
     4141 gcccattcgc gcgagatcga tgccgcgccg aggccggcta gccacgcctg acgcgatgcg
     4201 aacagggctg attcggccag cgcgttggcg ctgactacgc tgcgattgaa cgaaacttgc
     4261 ttggacatgg tgtgctcctt gggttgagtg ctcgaacgat attgccgaac agcttcgact
     4321 ttagccgttg gcgttagagc aatcacccta atcgcggagt gccgtccggg tcggggcgag
     4381 acgctgtgcc agcgccgacg cgagcttggc cacaatcccg tagatcgaaa gctgtggatt
     4441 ggcgccgacg ctggtcggga acagcgaacc gtcgaatacg tagaggttgt ccagatgatg
     4501 atgacggcca tcgtggtccg taacagcgcg acgggagtcg gctccgagcg ggcaaccacc
     4561 catcacgtgc gcgctgacga ccgccgtggc gagcggtttg agaggcaagc cagcgacacc
     4621 tgactgcgcc tcgctccagt ccgcgtaact cgcgcccgcg gcatgaaccg gcatgaccgt
     4681 cgtcgcaccg gcggcgaact ggatctcggc catcgtcgcg agcgcgcgtc gcgcaccatc
     4741 ccagaaataa tcgttgagcg gatagtccag cgacggcgta ccgtcgccct gaagcgaaac
     4801 cgtgccacct tcgctttgcg cgtgaaagcc gtcgcgcatc aatgcgatca gcacctgcat
     4861 ccgcgggagc tcgcgcatcc agcgcgcgtg cgatgcgccg tcccctgaca gtgtggtggc
     4921 gaccagcacc ggatgcagcg gcggcgcttc gagcttgtag ccgatcgggc cgtcgacggg
     4981 cagcgaatcg aggaaatggt ccgagtagac ggtctgcggt gcacccgcgt agccgtcgac
     5041 tcgatgcggc atcagcgcgg cggagacgac ggtcgggtgc agaaacgtgc gcttgccgac
     5101 gat
//
LOCUS       DATANS010000286         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_810840, whole genome shotgun sequence.
ACCESSION   DATANS010000286 DATANS010000000
VERSION     DATANS010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810840"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1585
                     /locus_tag="VHT22_04095"
     CDS             <1..>1585
                     /locus_tag="VHT22_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX3136767.1"
                     /translation="GNRTGPVSWWLSANHLHADTQPLNIITALRPAAPSAAGAPTTGA
                     FMDFNRLGQPIAVLGAGGLEHKNQDNLKAKLALDFTPEWRGTYTVGFFQNDAKSTVDT
                     YLRDAAGNPVYAGASLNIGGYNYNIPASSFSSSNGFYNIKQEHTAQSLALRSNTQRAW
                     DWEVVASNMRFGLDTTRVPTTALPAAEAGGAGTIHALDGTGWSTFDLKGIWRPQGLTG
                     AHHVSFGAHADRYKLNNVTSATANWISGAAAATTALALGRTRTDALWIQDAWRFAPRF
                     KLTLGGRQESWRAYDGLNFSAAPASNVSQPGLSAAKFSPKAAIAWDASTDWRVTASYG
                     TAHRFPTVTELYQAVTVAGVIFTPNPNLRPERAYSGELALERVIANGRVRVSLFQENL
                     TDALIAQNSTIPGTNTIGSSTQNIDRIRSRGVEIVAEKTDAMVRGLDVSGSVTFVDSR
                     ILADPTFRNAAGVLTDVTGNYTPNVPRLKSTAVATYRYDEHWSGTLAARYSARAWATL
                     DNTDINPNTYQGFAQYFVMD"
BASE COUNT          298 a          523 c          515 g          249 t
ORIGIN      
        1 cggcaatcgc acagggcccg tttcgtggtg gctgagcgcg aaccacctgc atgcggacac
       61 gcagccgctc aacatcatca ccgcgttgcg gcccgccgca ccgagcgccg ccggtgcgcc
      121 gacaaccggc gcgttcatgg atttcaaccg gctggggcag ccgattgcgg tgctcggcgc
      181 gggcggtctc gagcacaaga atcaggacaa cctcaaggcc aagctcgcgc tcgacttcac
      241 gcccgagtgg cgcggcacct acacggtcgg gtttttccag aacgatgcga aatcgacggt
      301 cgatacgtat ctgcgcgacg cggcgggcaa tccggtgtac gcaggcgcga gcctcaatat
      361 cggcggctac aactacaaca ttcccgcgag ctcgttctcc agctcaaacg gtttctacaa
      421 tatcaagcag gaacacaccg cgcagagcct ggcgctgaga tcgaacacgc agcgcgcatg
      481 ggactgggaa gtcgtagcca gcaatatgcg cttcggcctc gatacgacgc gcgtaccgac
      541 gacggcattg ccggcggccg aagccggcgg cgccggaacg atccatgcac tggacggcac
      601 gggctggtcg acgttcgacc tgaagggcat ctggcggccg cagggcctca cgggcgcgca
      661 ccatgtgagt ttcggcgcgc atgccgatcg ctacaaactg aacaacgtga cgtccgcgac
      721 cgccaactgg atctccggcg ccgcggcggc gacgactgcg ctggcgctgg gcaggacgag
      781 gaccgacgca ctgtggatcc aggacgcgtg gcgttttgcg ccgcgcttca agctgacatt
      841 gggcgggcgc caggaatcgt ggcgcgcata cgacgggctg aatttctcag cggcgccggc
      901 gtcgaacgtg agccagcccg ggctcagcgc cgcgaagttt tcgcccaagg cggcgatcgc
      961 gtgggatgcc agcacggatt ggcgcgtaac ggcgtcgtac ggcaccgcgc accggtttcc
     1021 gaccgtcacc gagctgtacc aggcggtgac ggtcgcgggc gtgatcttta cgcccaaccc
     1081 caatttgcgg cccgagcgcg cgtattccgg cgaactggcg ctcgagcgcg tgatcgcgaa
     1141 cggccgcgtg cgggtgtcgc tgttccagga aaatctcacc gacgcgctga tcgcgcagaa
     1201 ttcgaccata cccggcacca acacgatcgg ctcgtcgacg cagaacatcg accgcatccg
     1261 ttcgcgcggc gtcgagatcg tcgcggaaaa aaccgatgcg atggtgcgcg ggctcgatgt
     1321 gtccggcagc gtgaccttcg tcgattcgcg catcctcgcc gatcccacgt tcaggaatgc
     1381 ggcgggtgtg ctgaccgacg tcaccggcaa ttacacgccc aacgttccgc ggcttaaatc
     1441 gaccgcggtc gcgacctacc ggtacgatga gcactggtcg ggcacgttgg cggcgcgcta
     1501 tagcgcccgc gcgtgggcga cgctcgacaa cacggacatc aatccgaaca cctaccaggg
     1561 gttcgcccag tatttcgtca tggac
//
LOCUS       DATANS010000287         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_224622, whole genome shotgun sequence.
ACCESSION   DATANS010000287 DATANS010000000
VERSION     DATANS010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224622"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1491
                     /gene="purH"
                     /locus_tag="VHT22_04100"
     CDS             <1..1491
                     /gene="purH"
                     /locus_tag="VHT22_04100"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX3136768.1"
                     /translation="LVSTGGTAKAIAAAGLQVKDVSELTGFPEMMDGRVKTLHPKVHG
                     GLLAIRDNKEHAEAMKAHGIAPIDLLVVNLYPFEETVDKGAGYEDCIENIDIGGPAMI
                     RAAAKNHDDVAVVVEAQDYQAVLNELAANQGATTLSLRRRLAAKAYARTAAYDAAISN
                     WFALQLKDDAPDFRAFGGRLIQSLRYGENPHQTAAFYRTPDKRPGVATARQLQGKELS
                     YNNINDTDAAYECIGEFDPKRTAACVIVKHANPCGVAEGADLVSAYRKAFACDTQSPY
                     GGIIAVNRTLDAEAARVITEILTEVIIAPDATEEAIAIIAKRKNLRLLLAGSLPDPRA
                     SGLTAKTVAGGLLVQSRDNAVVDDITLKTVTKRAPTDAELRDLKFAFRVAKHVKSNTI
                     IYAKDLATVGIGAGQMSRVDAARIAARKAQDAAADAKLAEPLTKGSVVASDAFFPFAD
                     GMLACIEAGATAVIQPGGSIRDDEVIKAADEHGIAMVFTGVRHFRH"
BASE COUNT          301 a          537 c          472 g          231 t
ORIGIN      
        1 ctcgtctcca cgggcggcac cgcgaaggcg atcgcggcgg ctggactgca ggtcaaggac
       61 gtctcggagc tgacgggctt tcccgaaatg atggacgggc gggtcaagac cctgcacccc
      121 aaggtgcatg gcggcctgct cgcgatccgc gataacaagg aacatgccga agccatgaag
      181 gcgcacggta ttgcgccgat cgatttgctc gtggtcaacc tctacccgtt cgaggaaacc
      241 gtcgacaaag gcgcgggcta cgaagattgc atcgagaaca tcgacatcgg cggtcccgcg
      301 atgatccgcg ccgccgcgaa gaaccatgac gacgtcgccg tcgtggtcga ggcacaggac
      361 tatcaggccg tgctcaacga gctcgccgcc aaccagggcg caacgacgct ttcgctgcgc
      421 cgccgcctcg ccgcaaaagc ctatgcgcgg accgccgcct atgacgccgc gatctcgaac
      481 tggttcgcgt tgcagctcaa ggacgacgcg ccggatttcc gcgcctttgg cggccggctg
      541 atccagtcgc tgcgctatgg cgagaacccg caccagaccg ccgcgttcta tcgtacgccc
      601 gacaagcgcc ccggcgtcgc caccgcgcga cagttgcagg gcaaggaact gtcctacaac
      661 aacatcaacg ataccgatgc ggcctatgaa tgcatcggcg aattcgatcc gaagcgcacc
      721 gcggcctgcg tcatcgtcaa gcacgccaat ccctgcggcg tcgccgaagg cgccgatctg
      781 gtcagcgcct atcgcaaggc gttcgcctgc gatacgcagt cgccctatgg cggcatcatc
      841 gccgtcaatc ggacgctcga cgccgaagcc gcccgtgtca tcacggaaat cctgaccgaa
      901 gtgatcatcg cgcccgacgc gacggaagaa gccatcgcca tcatcgcgaa gcgcaagaac
      961 ctccggcttc tcctcgccgg cagcctgccc gatccgcgcg cgtcgggcct cacggcaaag
     1021 accgtcgcgg ggggtctcct cgtgcagagc cgtgacaacg cggtagtcga tgacatcacg
     1081 ctgaagacgg tgaccaagcg tgcgccgacc gacgccgaac tgcgcgatct caaattcgcc
     1141 ttccgcgtcg ccaagcacgt caagtcgaac accatcatct atgccaagga tctcgccacc
     1201 gtcggcatcg gcgccggcca gatgagccgg gtcgatgccg cgcgcatcgc cgcccgcaag
     1261 gcgcaggatg ccgcggctga cgccaaactc gccgaacccc tgaccaaagg ctcggtcgtc
     1321 gcgtcagacg cgttcttccc gttcgccgac ggcatgctgg cctgcatcga agccggcgcc
     1381 accgccgtga ttcagcccgg cggctcgatc cgcgatgacg aagtgatcaa ggccgccgac
     1441 gagcatggca tcgccatggt gttcacgggc gtgcggcatt tccggcattg agccctcaac
     1501 gagcgtagcc cgggtgagcg aagcgacccc ggggacagtg t
//
LOCUS       DATANS010000288         1699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_99519, whole genome shotgun sequence.
ACCESSION   DATANS010000288 DATANS010000000
VERSION     DATANS010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99519"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VHT22_04105"
     CDS             <1..616
                     /locus_tag="VHT22_04105"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3136769.1"
                     /translation="VVIEADIHRPADNLRVVETAVAAFGRLDILINNDGAPPLGALLE
                     FDDAAWQKAIQQNLMSVIRACGAAIPHMRKTGGGRIINITAISARQPAAGFGLSVATW
                     AGLIGYAKTLSLEVGPDNITVNTICPGRIDTPLSQRGFERAAKGDPARAKEMLDTLVR
                     NTPVRRIGVPEDIAGVVAFLASAQASFITGVTLPVDGGRAAALL"
     gene            complement(676..1233)
                     /locus_tag="VHT22_04110"
     CDS             complement(676..1233)
                     /locus_tag="VHT22_04110"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX3136770.1"
                     /translation="MSNNRKVIASLIAAAALAASGSLVYAQQQKPGDSAVQSRGEGER
                     HAMRGHHGGGHGQYSNPAARVEGHLAALKVELKITPAQEKAWQTFADKTRKQAEARAA
                     QRAKFQGAKPAENMPAPERLAQRTARMKQAIAGMEARTAAVTELYAVLSPEQKALADK
                     QFARGHRGGRFHRGGHDHQRSKSNA"
     gene            1466..>1699
                     /locus_tag="VHT22_04115"
     CDS             1466..>1699
                     /locus_tag="VHT22_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3136771.1"
                     /translation="MSAQDHVLVVDDDLDIRSLLRDYLEKNGYRVTAVGDGKAMWAAF
                     DEVRPDILVLDVMLPGTDGLTLCRNVRARSGIPI"
BASE COUNT          271 a          551 c          549 g          328 t
ORIGIN      
        1 ggttgtgatt gaggcggaca tacacaggcc ggccgacaac ctgcgcgtag tcgagaccgc
       61 ggtcgcggcg ttcggccggc tcgacatcct catcaacaac gacggcgcgc cgccgctcgg
      121 cgctttgctc gagttcgacg acgccgcatg gcagaaagca atccagcaaa acctgatgag
      181 cgtgatccgc gcgtgcggcg ccgcgatccc gcacatgcgc aaaacgggcg gcggccgcat
      241 catcaacatc accgcgatct cggcgcgcca accggccgcg ggcttcggct tgtccgtggc
      301 gacgtgggcc ggattgatcg gctacgcgaa aaccctgtcg ctcgaagtcg ggccggacaa
      361 catcacggtc aatacaatat gcccgggccg catcgacacg ccgctgtcgc agcgcggttt
      421 cgagcgggcg gcgaagggcg atccagcacg cgcaaaggaa atgctcgaca cgctggtgcg
      481 caataccccg gtccggcgta tcggcgttcc ggaagatatt gccggcgtag ttgcctttct
      541 cgcgtcggcg caggcctcgt ttatcaccgg cgtcacgctg cccgtcgatg gcggccgtgc
      601 ggcggcgctg ttgtagcggg taaaaaaaag cgccggcacc ttgcggcacc ggcgcccttc
      661 actcgcaatt acggcttaag cgttcgattt actacgctga tggtcgtgcc cgccgcgatg
      721 gaaccggcca ccgcgatgac cgcgcgcgaa ctgcttgtcg gcaagcgctt tctgttccgg
      781 gctcagcacg gcatagagct ccgtgaccgc cgcggtgcgc gcttccatgc ccgcgatcgc
      841 ctgcttcatg cgcgcggtgc gttgcgcgag gcgttccggt gccggcatgt tctccgccgg
      901 tttggcacct tggaatttcg cgcgctgcgc ggcgcgcgct tcggcttgct tgcgcgtttt
      961 gtcggcgaag gtctgccaag ctttttcctg ggccggggtg atcttcagct cgactttcag
     1021 cgcggcaaga tggccttcga cgcgcgcggc cggattgctg tattggccgt gcccgccgcc
     1081 atgatggccg cgcatggcgt ggcgctcgcc ctcaccgcgg gactggaccg cgctgtcgcc
     1141 gggcttctgc tgctgggcgt aaacgagcga acccgacgcg gcgagcgctg cggcggcgat
     1201 cagacttgca atgactttac ggttgttgct catgctcgat ctcctttgcg ttaatgtatg
     1261 ttgcctcggg ggagccgttt acagcggccc tttttggctg ggatcatttg agcatccact
     1321 tgtaacccgg acatttcagg cagcggcatt tttgtatctc acgctgtcgc cgggccgggt
     1381 tgttacactg cgatacaaat ttttagccgg gccggcaggc gcggctgtat aaagatccca
     1441 tcgccatccg cccgtgcccc gaaccatgag tgcccaagac cacgtgctcg tcgtcgacga
     1501 cgatcttgac atccgcagtc tcctgcgcga ctacctcgaa aagaacggct accgcgtgac
     1561 cgcggtgggc gacggcaaag ccatgtgggc ggcgttcgac gaagtgcgcc cggacatcct
     1621 ggtgctcgac gtgatgctgc cgggcacgga cgggctcacg ctgtgccgca acgtgcgcgc
     1681 gcgctccgga ataccgatc
//
LOCUS       DATANS010000289         2340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_687414, whole genome shotgun sequence.
ACCESSION   DATANS010000289 DATANS010000000
VERSION     DATANS010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687414"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VHT22_04120"
     CDS             complement(<1..1008)
                     /locus_tag="VHT22_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX3136772.1"
                     /translation="MKKSAGLYLSAREASSELAISQATLYAYVSRGLIRSEPSQDSRS
                     HRYRAEDVRTLKERRTPAPEPRGMRSFDADLPVMDSAVGTITEDGAIYRGVNCIDLAE
                     RDTLEHAATLLWDVAGVDPFDQNNCPIVSDEMRAVAEAARQANAIDRAIAVLAFAARA
                     DPRAFTRADEERAMLGGRIMRLVVATMLNAKSSAKPLHLQIARVWAPEHKHAADLIRR
                     ALVLLADHELNASTFTVRCAASTGLNLYDAMIAGLVALKGPKHGGAGVLAAQLLKTLA
                     HGEVAPIIRERVALGERFAGFGHGVYKHGDPRARALLEALARSGADRKLTHEIPDRIA
                     EA"
     gene            1083..2174
                     /locus_tag="VHT22_04125"
     CDS             1083..2174
                     /locus_tag="VHT22_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase/methylcitrate synthase"
                     /protein_id="HEX3136773.1"
                     /translation="MNIQLSKTPIGLDGIPAAETVLSHVDGERGELIIAGERVGDLAR
                     KTGFEGVTARLWSGGTGQPVGEASVRAALGAGRERACARLPDLLGITRGLSIVDGFRA
                     AIAGLRAEAGLAHEATIVGAFPVIAGALVQRVKGNDPIAPDPIASHAADTLSMMLGRK
                     PDAREAAALDAYFVTVCDHGMNASTFTTRVVASTQADLFAAVTAGYCALTGPLHGGAP
                     EPVLEMLDAIGSTERIKPWVDSALASGERLMGFGHRVYRVRDPRADVLKEAIERLAGE
                     GTDLPFAGEVEAYIRGALRIKNPERPLDTNVEFFTAILLDALKIPRQAFTPIFAVARA
                     AGWTAHAREQQRGGRLIRPSSAYIGPMPG"
BASE COUNT          396 a          744 c          806 g          394 t
ORIGIN      
        1 cgcctccgcg atcctgtcag ggatttcgtg ggtaagtttg cgatccgcgc cggagcgcgc
       61 cagcgcttcc agcagcgccc gcgcgcgggg atcgccatgc ttgtagacgc catggccgaa
      121 ccccgcgaaa cgctcgccga gcgcgacacg ctcgcggatg ataggggcaa cctccccgtg
      181 agcgagcgtc ttcagaagct gcgccgccag cacgccggcg ccgccgtgct tcgggccctt
      241 gagcgcgaca agcccggcga tcatggcgtc gtagagattc aatccggtcg acgccgcgca
      301 gcgcacggtg aaggtcgagg cattcaattc atgatccgcc agcagcacca gcgcgcggcg
      361 gatcagatca gcggcgtgtt tgtgctctgg cgcccagacg cgcgcgatct gtaaatgaag
      421 cggttttgcc gaggatttcg cgttcagcat ggtcgcaacc acgagccgca tgatgcgacc
      481 acccagcatc gcgcgctcct cgtcagcgcg ggtgaaggcc cttgggtcgg ccctggcggc
      541 gaacgccagc acggcgatgg cgcgatcgat cgcattggcc tggcgcgccg cctcggccac
      601 ggcgcgcatt tcgtccgaca cgatggggca attgttctga tcgaagggat cgacgccggc
      661 gacatcccac aacagcgtcg cggcatgttc gagcgtgtcg cgttcggcaa gatcgatgca
      721 gttgacgccg cgatagatcg cgccgtcctc ggtaatcgtt ccgaccgccg aatccatcac
      781 gggaagatcg gcgtcgaagc tgcgcatgcc gcgcggttcg ggcgccggcg tgcgccgctc
      841 cttcagcgtg cggacgtcct cggcccggta gcggtggctg cgggaatcct gtgacggctc
      901 cgagcggatc agcccgcggc tgacataggc gtagagcgtc gcctgcgaga tcgcgagttc
      961 ggaagaggcc tcgcgggccg agagataaag gccggcggat tttttcatat tgatttacat
     1021 aatcaagatt gatcaatctg tcgagaggcc tcaccctttg cactgcacaa aggagaagcc
     1081 ttatgaacat ccagctttcg aagaccccga tcggcctgga cgggattccg gccgccgaaa
     1141 ccgtgctcag ccatgtcgat ggcgagcgcg gcgaactgat catcgccggc gagcgggtcg
     1201 gcgacctcgc ccgcaagacc ggttttgagg gtgtgaccgc ccggctgtgg agtggcggaa
     1261 ccggccagcc ggtcggcgaa gcctccgtgc gggccgccct cggcgcgggg cgcgaacgcg
     1321 cctgtgctcg gctgccggat ctcttgggca tcacgcgcgg cctatcgatc gtcgacggtt
     1381 ttcgcgcggc gatcgccggg ctgcgcgcgg aagcgggtct ggcacacgag gccaccatcg
     1441 tcggcgcgtt tccggtcatc gccggtgcgc tggtgcagcg ggtgaaaggg aacgacccca
     1501 ttgcacccga tccgatcgcg agccatgccg ccgacacgct gtcgatgatg ctgggccgca
     1561 agcccgatgc gcgcgaggcg gcggcgctcg acgcctattt cgtgacggtg tgcgaccacg
     1621 gcatgaacgc gtcgaccttc acgacgcgcg tggtggcctc gacacaggcg gatttgttcg
     1681 cggccgtcac cgccggctat tgcgcactga ccgggccgct gcatggcggc gcacctgaac
     1741 cggtgctgga aatgctggac gcgatcggct cgaccgagcg catcaagcct tgggtagaca
     1801 gtgcgctggc aagcggcgag cggctgatgg gattcggtca ccgcgtctat cgggtccgcg
     1861 atccccgcgc cgatgtgctg aaggaagcga tcgagcggct tgcgggggaa ggcacggacc
     1921 tgccgtttgc cggcgaggtc gaggcctaca ttcgcggcgc gttgcggata aagaatccgg
     1981 agcgcccgct cgacaccaat gtggagttct tcaccgcgat cctgctcgac gcgctgaaaa
     2041 tcccacggca ggcgtttacg ccgatatttg cagtggcgcg cgccgccggc tggaccgcgc
     2101 atgcccgcga acagcagcgc ggcgggcggc tgattcggcc gagttctgcg tatatcgggc
     2161 cgatgccggg atgagtgggg actactgaaa gcggaggcca tactccgctg tcgtccctgc
     2221 gaacgcaggg acccataacc acaggtgttc atggttgggg catgctggag ctccaacctt
     2281 cgcaaaacca agtttggggg ttatgggtcc ccgcgttcgc ggggacgaca gctacggaga
//
LOCUS       DATANS010000290         4110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_184245, whole genome shotgun sequence.
ACCESSION   DATANS010000290 DATANS010000000
VERSION     DATANS010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184245"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1209)
                     /gene="clsB"
                     /locus_tag="VHT22_04130"
     CDS             complement(22..1209)
                     /gene="clsB"
                     /locus_tag="VHT22_04130"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HEX3136774.1"
                     /translation="MNRFLPGNRIILLSNGDQYFPALIGAIDAARETVWFETYIFADD
                     ATGRLVAGALIRAAQRGVNVRALVDGWGAKFYLTVALEREMRDGGVDFQKYRPEVAPW
                     QFRSHRLRRLHRKLCVVDGEIAFVGGINVIDDENTPRQTPPRIDFAVSVEGPLLPEIE
                     QTMQRVWGIVQLVRTGTSELPLLFPEPRRAPRVGTQTARFVIRDNLRHRRDIEHAYLA
                     AIRTAKREIILANSYFFPGIRIRRALVSAAQRGVAVTLLLPGRVEYLLLHLATRAMYG
                     QLLQAGITIEEYHRSMLHAKVAVVDDIWATVGSSNIDPYSLLMAREANVIVRDRGFNE
                     KLKGQLRELIATGSRRVVPDDWHSRSRPYKAAIWIAYGIVRMAMGLVGYGGNDWFTES
                     AKD"
     gene            complement(1228..1995)
                     /locus_tag="VHT22_04135"
     CDS             complement(1228..1995)
                     /locus_tag="VHT22_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX3136775.1"
                     /translation="MRFTVATYNIHKGFSHLTRRVVIHELRDKLHGLDADVMFLQEVQ
                     GVHRRHAGRYRDWPLKPQHEFIADRVWREVAYGRNAITRHGHHGNAVLSRFPIIEQAN
                     QDISAHAFESRGMLHCVMQLQRGLPPLHCINVHLGLFERGRLWQIGALCERIRESVPA
                     GAALIIAGDFNDWRRKADRMLQEELGVVEVFEAARGRPARTFPSVLPVFRLDRIYARG
                     LKVVAADVHYAYPLARLSDHAALAATFDLTRSSVASR"
     gene            2219..2485
                     /locus_tag="VHT22_04140"
     CDS             2219..2485
                     /locus_tag="VHT22_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HEX3136776.1"
                     /translation="MRQQTTIQAAIAGLLALGFATAVSAQPVPEQKGKDKCYGIAKAG
                     QNDCAAGKHACAGQSTVDNDPVSWKYVAKGTCEKVGGKTTAPKS"
     gene            2552..3394
                     /locus_tag="VHT22_04145"
     CDS             2552..3394
                     /locus_tag="VHT22_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEX3136777.1"
                     /translation="MVHARVLPAVAGIGLRAPHVGEVRDSRPPIGWLEVHSENYFVDG
                     GPALAALEAIRADYPVSLHGVGLSLGSADPLDAEHLARLKQLASQIEPSAVSEHLCWS
                     HVDGRHLNDLLPLPLTDESLALVCNRIDAVQSALGRELLVENVSSYLRFASDTIAEWD
                     FVAAVARKSGCKLLLDVNNVYINAVNHGFDPHAYLATIPGESVAEIHLAGYDASGLCL
                     IDTHGSRVAAPVWALYRDAIKRFGAKPTLLEWDTDIPALDVLLDEAAQAQAILDEHRY
                     AIAR"
     gene            3378..>4110
                     /locus_tag="VHT22_04150"
     CDS             3378..>4110
                     /locus_tag="VHT22_04150"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEX3136778.1"
                     /translation="MPSLVEIQRGVAAAMISGDADALAGLGIVAGTISPAARIAIYRS
                     NVFGNYRKALATTFPVVRQLVGAAFFNAAADDYVRAHPSTRGDVNRYGDDLARFLGAY
                     EPARALAYLPDVARLEWAIDQAAIAADAPPFDLAALAALPEKAHAGLRFVLHPSAQLI
                     ESHYPILHIWQVNQADFEGPAEVDLREGGDSLLVVRGECGLDLQRLEPGEAALLKGFA
                     AGETLAVAADLAIEAEATFDLPAALR"
BASE COUNT          703 a         1371 c         1352 g          684 t
ORIGIN      
        1 gatcgcgccg ctgtgagggc gtcagtcctt ggcgctctcg gtgaaccagt cgttgccgcc
       61 gtagccgacc aaacccatcg ccatccgcac gatcccatag gcgatccaga tcgccgcctt
      121 gtagggtctc gaccggctat gccagtcgtc ggggacgacg cggcgcgacc cggtggcgat
      181 cagctcgcgc agctggccct tcagcttttc gttgaaaccg cgatcgcgga cgatcacatt
      241 ggcctcgcgc gccatcagca ggctgtacgg atcgatgttc gacgagccga cggtggccca
      301 gatatcgtcg acgacggcga cctttgcgtg cagcatgctg cgatggtatt cctcgatcgt
      361 gatgccggcc tgaagcagct ggccgtacat cgcacgcgta gccagatgca gcagcaggta
      421 ctcgacccgc cccggcagca gcaaggtcac cgcgacgccg cgctgggcgg ctgagaccag
      481 cgctcgccgg atgcgaatcc cgggaaagaa gtacgagttg gcgaggatga tctcgcgctt
      541 cgcggtgcgg atcgcggcga ggtacgcgtg ctcgatgtcg cggcgatgcc gcagattgtc
      601 gcggatgacg aatctggcgg tctgcgtgcc gacgcgcggg gctcggcgag gctcgggaaa
      661 caacagcggg agctccgacg ttcccgttct caccaattgc acgattcccc acacgcgctg
      721 catcgtctgc tctatctcgg gcagcagcgg gccttcgacg gaaaccgcga agtcgatccg
      781 gggcggggtt tgccgcggcg tgttttcgtc gtcgatgacg ttgatgccgc cgacgaaagc
      841 gatctccccg tcgaccacgc acagtttccg atgcaggcgg cgcaggcgat gcgagcggaa
      901 ttgccagggt gcgacctcgg gccggtactt ctgaaaatcg acgccgccgt cgcgcatttc
      961 gcgttccagc gcgacggtca ggtagaactt ggcaccccag ccgtcgacga gcgcgcgcac
     1021 attcacgcct cgctgggcgg cccggatcaa cgcgccggcg accagccgac ccgtcgcgtc
     1081 gtcggcgaag atatacgtct cgaaccacac ggtctcgcga gcggcgtcga tcgcgcctat
     1141 cagtgccgga aaatactggt caccgttgct cagcaggatg atgcggttgc ccggcaggaa
     1201 gcgattcatc gcgccgcggg ctggcgatca gcgcgacgcg acgctggaac gcgtaaggtc
     1261 gaacgtcgcg gcaagcgccg catggtcgga cagcctcgcc agcggatagg catagtgcac
     1321 gtcggcggcg acgaccttga gtcctcgcgc atagatgcga tcgagccgga acacgggcag
     1381 gactgagggg aacgtgcgcg ccggccgtcc gcgcgccgcc tcgaacactt cgacgacgcc
     1441 gagctcctcc tgcagcatcc ggtcggcctt gcgccgccaa tcgttgaaat cgccggcgat
     1501 aatcagcgcg gcgccggccg gaaccgactc gcggatccgc tcgcatagcg cccctatctg
     1561 ccagagccga ccgcgctcga acagcccgag gtggacgttg atgcaatgca gcggcggcag
     1621 cccgcgctgc agctgcatta cgcaatgcag catgccgcgg ctctcgaacg cgtgcgccga
     1681 aatgtcctgg ttcgcctgct cgatgatcgg aaagcgcgaa agcaccgcat tgccgtggtg
     1741 gccgtgacgc gttatcgcat tgcgcccata ggcgacttcg cgccagaccc tgtcggcgat
     1801 gaactcatgc tggggcttca gcggccagtc gcgataacgg ccggcgtgcc ggcgatgcac
     1861 gccctgcact tcctgaagga acatcacgtc ggcatccagg ccatgcagct tgtcgcgcaa
     1921 ctcgtggatg acgacccgcc gtgtcaggtg ggaaaatccc ttgtggatgt tgtaggtagc
     1981 gacggtaaaa cgcatgatgg cagtatggcc gcgggaagtc ccttatggta acggcaattc
     2041 gccgcgatcg tcgccgcggg tgccgacagg cgctgcggaa tcctgcctgg cggccggact
     2101 cgcgaccgca tctgtcgcgg gcaatcgtgt aaggtagccg gtatcggcgc gaccaatgcc
     2161 acgcatgggc gagcatgtcg gtcatgcacc gattctggtc atcgcacagg agatggcaat
     2221 gagacagcaa accacgattc aagccgcgat cgcgggactg ttggcgctgg gttttgcgac
     2281 cgcggtgtcg gcccagccag ttcccgaaca gaagggcaag gacaagtgct acggcatcgc
     2341 caaggccggc cagaacgact gcgccgccgg caagcacgcc tgcgccggcc aaagcaccgt
     2401 cgacaacgat ccggtctcat ggaagtacgt cgccaaaggg acctgcgaaa aggtcggcgg
     2461 caagacgacc gcgccgaagt cctgatcgca ggcttcgcga ttctgcgtcg tccgctgacc
     2521 gcgggcggcg cattctgacc tgttgcccga gatggtccat gcgcgcgtac ttccagccgt
     2581 cgccggcatc ggcttgcgcg ctccgcacgt cggcgaggtt cgcgactcgc ggccaccgat
     2641 cggatggctc gaggtgcaca gcgaaaacta ttttgtcgac ggcggcccgg cgcttgccgc
     2701 gctcgaggcg atacgcgccg actatccggt gtcgctgcac ggcgtaggcc tgtcgctggg
     2761 ctcggcagac ccgctggacg ccgagcacct ggcgcgcctg aagcagctcg cctcgcagat
     2821 cgaaccgtcg gcggtctccg agcatttgtg ctggagccac gtcgacggcc gccatctgaa
     2881 cgatctgctg ccgctaccgc tgaccgatga gtcgctcgcg ctggtatgca atcgcatcga
     2941 cgcggtgcaa tccgcgctcg gtcgcgagct gctggtggag aacgtctcgt cctatttacg
     3001 cttcgccagc gatacgatcg ccgagtggga cttcgtcgcc gccgtcgcac gcaaaagcgg
     3061 ctgcaagctg ctgctcgacg tcaataacgt ctacatcaac gcggtcaatc acggcttcga
     3121 cccgcacgcc tacctcgcga cgatccccgg cgagagcgtg gccgagatcc acctcgccgg
     3181 ctacgatgcc agcgggctgt gcctgatcga cacccacggg tcgcgggtcg cagctccggt
     3241 ctgggcgctg taccgcgacg cgatcaagcg tttcggggcc aaaccgacct tgttggagtg
     3301 ggacaccgac attcccgcgc tcgacgtgct gctggacgaa gcggcgcaag ctcaggcgat
     3361 cctcgacgag catcgctatg ccatcgctcg ttgagattca gcgcggcgtt gccgctgcga
     3421 tgatatccgg cgatgcagac gcgctcgccg ggctgggtat cgtcgccggc acgatcagcc
     3481 ccgcggcgcg catagccatc taccgcagca acgtgttcgg caactaccgc aaggcgctgg
     3541 cgactacgtt tccggtggtc aggcagctgg tcggcgccgc gttcttcaac gccgcggccg
     3601 acgactatgt gcgcgcgcat ccttcgaccc gcggcgacgt caaccgctac ggcgacgatc
     3661 tggcacgctt cctcggcgct tacgagcctg cgcgagcgct cgcctacctg ccggacgtcg
     3721 cgcggctcga gtgggcgatc gatcaggcgg ccatcgcggc cgacgcgccg ccattcgatc
     3781 tcgcggcgct ggccgcgctg ccggaaaaag cccacgccgg cctgcgcttc gtgctgcatc
     3841 cgtcggcgca gctcatcgaa tcgcattatc cgattctgca catctggcag gtcaaccagg
     3901 cggacttcga agggccggcc gaggtcgatc tgcgcgaggg cggcgactcg ctgctggtcg
     3961 tccggggcga gtgcggcctc gacctccagc ggctcgagcc cggcgaagcg gctctgctta
     4021 aaggcttcgc cgccggcgag actctcgctg ttgcggcgga tctcgcgatt gaagccgaag
     4081 cgacattcga cctgcccgcc gcgctgcggc
//
LOCUS       DATANS010000291         1579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_519068, whole genome shotgun sequence.
ACCESSION   DATANS010000291 DATANS010000000
VERSION     DATANS010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519068"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="VHT22_04155"
     CDS             <1..1184
                     /locus_tag="VHT22_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX3136779.1"
                     /translation="ALREQVDASVDNGALPLSGLEVSAGALDVFGRRLNELRVGASRS
                     GNNWQLDLRGRELEGMARWEPAAPAHPNGRIVARLQRLTPPTAASSHAAPTATSPSEV
                     NSWPEIDLTAETLRLKERDLGRLELNAQPRGADWRIDSLKLINEDGRLDANGWWRGGG
                     GNEQTTLDADLDVHDAGKYLARYALPDAVRDAATRIRGQVSWKGGPQDFDYPTLNGKL
                     RLETGRGQFTKLDPGLGKLLGVLSLQSLKRRLAFDFQDLFAEGFAFDEITGDVRIQNG
                     VMKSDNLKIVGPSARVAIQGEADIARETQHLSVRVQPTLSAGVSVGAAALLLANPVIG
                     AAVYAGSLLAQKAMQDPIEQMFSFGYTVTGSWSDPVVEREGRFPTPAAASASKPDTPP
                     Q"
     gene            1181..>1579
                     /locus_tag="VHT22_04160"
     CDS             1181..>1579
                     /locus_tag="VHT22_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX3136780.1"
                     /translation="MSAAGGVDSIRVAAVQTVSGSDVGANLAAVEPLIVAAREQGAAL
                     VLLPEYFGIFGARATDKVDAREADGHGPQQDFLSRVAREQRLWLIGGTVPIATADPAR
                     VRSASLVFDPHGQQVARYDKIHLFAFSQGDE"
BASE COUNT          253 a          522 c          566 g          238 t
ORIGIN      
        1 tcgcgctgcg cgagcaggtg gacgccagcg tcgacaacgg cgcgttgccg ctgtcgggtc
       61 tcgaagtcag tgccggtgca ctagacgtgt tcggccggcg gctcaacgaa ttgcgcgtcg
      121 gcgcgagtcg cagcggcaac aactggcaac tcgacctgcg agggcgcgag ctcgaaggca
      181 tggcgcgctg ggagccggcg gcgcccgcgc atccgaacgg ccgtatcgtc gcgcgcctgc
      241 aacggctgac gccgccgaca gcggcgtcgt cgcatgccgc gccgaccgcg acttcgccgt
      301 cggaagtgaa cagctggccg gagatcgacc tgaccgccga gactttgcgc ctgaaggagc
      361 gggacctggg acggctcgag ttgaacgcgc agccgcgcgg ggccgactgg cggatcgaca
      421 gcctgaagct gatcaacgag gacgggcggc tggacgccaa cggctggtgg cgcggcggcg
      481 gcggcaacga gcagacgacg ctggacgccg acctcgacgt gcacgatgcc ggcaagtacc
      541 tggcgcgcta cgcgctgccc gacgcggtcc gcgacgccgc gacgcggatc cgcggccagg
      601 tttcgtggaa gggcgggccg caggacttcg actatccgac gctcaatggc aagcttcgcc
      661 tcgaaaccgg gcgcggccag ttcaccaagc ttgatcccgg tctcggcaag ctgctgggcg
      721 ttctttcgct gcagtcgctg aagcgacggc tcgcgtttga tttccaggac ctgttcgccg
      781 agggcttcgc tttcgacgaa atcaccggcg acgtgcgcat ccagaacggc gtgatgaaaa
      841 gcgacaatct gaagatcgtc ggaccgtcgg cgcgcgtggc gatccagggc gaggcagaca
      901 tcgcgcgcga gacgcagcat ctgtccgtgc gcgtgcagcc gaccttgtcg gcaggcgtgt
      961 cggtcggcgc cgctgcgttg ttgctcgcca atccggtcat cggcgccgcc gtgtacgccg
     1021 gttcgctgct cgcacaaaag gcgatgcagg atccgatcga gcagatgttt tcgttcggct
     1081 atacggtgac cggcagctgg tcggacccgg tggtcgagcg cgaaggccga ttccctacgc
     1141 cggcggcagc ctcggcaagc aagccggata ccccgccgca atgagcgccg cgggcggggt
     1201 tgacagcatc cgcgtggccg cggtgcagac cgtgtccggc agcgacgtcg gtgcgaacct
     1261 ggccgcagtc gagccgctga tcgtcgcggc acgagagcag ggcgccgcgc tcgtcctgct
     1321 gcccgagtac ttcggcatct tcggcgcccg ggccactgac aaggtcgatg cgcgtgaggc
     1381 cgacggccac ggtccgcagc aggactttct gtctcgcgtc gcgcgcgagc agcggctgtg
     1441 gctgatcggc ggcaccgtgc cgatcgctac ggccgacccg gcgcgggtgc gcagcgcttc
     1501 gctggtattc gatccgcacg gccaacaggt tgcccgctac gacaagatcc atctgttcgc
     1561 attcagccag ggcgacgag
//
LOCUS       DATANS010000292         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_729604, whole genome shotgun sequence.
ACCESSION   DATANS010000292 DATANS010000000
VERSION     DATANS010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729604"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VHT22_04165"
     CDS             <1..744
                     /locus_tag="VHT22_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HEX3136781.1"
                     /translation="HEYGMTTMELNPIRMRADKRGRLTPVACDFKCGFDRDDPRWQRL
                     NLPVHLFAADYSNFEQEINQLRTHQGQSDVYVINDKGTVLAPTFGGGANSLVTEMLGD
                     DAIISSDFGGNPPYEKMKEVARICFKHWLKQANVLFIIGGKSNNTDIYETLRAMADAL
                     REHFSAHGPTPLYVVIGRGGPNRVRGMGALRDTCDALGLPYRLFGFDSDMSEVIQFAK
                     KVNTWMKAGGREQVAARLGIEAPVAASAN"
     gene            761..>1611
                     /locus_tag="VHT22_04170"
     CDS             761..>1611
                     /locus_tag="VHT22_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX3136782.1"
                     /translation="MLQSNWQKKGVGDFKYYVGISSLTQVASREDRVCVLNILGGESS
                     DVTPVSHAYSGGNVVFGTAPGKGGAVLETPIGSIPVFNNVRDGLDAGHRFNCGVVYLP
                     PSAARDGVAELIRVNPDLRKIFIITEKIAVHDAREIRAMGQQNGIDIFGANGLGVADS
                     WNQVRIGGALGGDKPGDSLKRGSIAIFSNSGGFSSTIAQYLRMAGWGTTTVISSGKDV
                     YIHYAAPEFAFALANDARSKAAVLYCEPGGYYELDAHFTKPVVACVVGRWKSKLTRAV
                     GHAGAMAG"
BASE COUNT          303 a          523 c          528 g          257 t
ORIGIN      
        1 cacgagtacg gcatgaccac gatggaactc aacccgatcc gcatgcgcgc cgacaagcgc
       61 ggtcgcctca cgccggtggc gtgcgacttc aagtgcggtt tcgatcgcga cgatccgcgc
      121 tggcagcggc tgaacctgcc ggtccacctg ttcgcggccg actactccaa cttcgagcag
      181 gagatcaacc agctgcgcac gcaccagggg cagagcgatg tctacgtcat caacgacaag
      241 ggtacggtgc tggcgcccac tttcggcggc ggcgccaact cgctggtgac cgagatgctg
      301 ggtgacgacg cgatcatcag ctccgacttc ggcggcaacc cgccctacga gaagatgaag
      361 gaggtggcgc gcatctgctt caagcactgg ctcaagcaag ccaacgtgct ctttatcatc
      421 ggcggcaaga gcaacaacac cgacatctac gagacgctgc gcgcgatggc cgacgcgctg
      481 cgcgagcact tcagcgcgca cgggccaacg ccgctctacg tggtgatagg ccgcggcgga
      541 ccgaaccgtg tgcgcggcat gggcgcgctg cgcgacacct gcgatgcgct ggggctgccg
      601 taccggctgt tcggcttcga ctcggacatg agcgaggtga tccagttcgc gaagaaggtc
      661 aacacgtgga tgaaggccgg cggacgcgaa caggtcgcgg cacggctcgg tatcgaggcc
      721 ccggtcgccg cctctgccaa ctgatcacgg agacatcacc atgttgcaaa gcaattggca
      781 gaaaaaaggc gtcggtgatt tcaagtacta cgtcggtatc agctcgctga cgcaagtcgc
      841 atcgcgcgag gaccgcgtct gcgtcctcaa catcctgggc ggcgaatcga gcgacgtgac
      901 gccggtcagc cacgcctact ctggcggtaa cgtcgtgttc ggcaccgcgc cgggcaaagg
      961 cggcgccgtt ctggaaacgc cgatcggcag catccccgtt ttcaacaacg tgcgcgacgg
     1021 cctggatgcg ggccaccgct tcaactgcgg cgtggtctac ctgccgccct cggcggcgcg
     1081 cgacggcgtg gccgaactga tccgcgtcaa tcccgatctg cgcaagatct tcatcatcac
     1141 cgagaagatc gccgtgcacg acgcacgcga gatccgcgca atgggccagc agaacggcat
     1201 cgacatcttc ggcgcgaacg gcctgggcgt ggcggactcc tggaaccagg tgcgcatcgg
     1261 cggcgcgctg ggcggtgaca agcccggcga ttcgctgaag cgtggctcga ttgccatctt
     1321 ctccaattcg ggcgggttct cgagcaccat cgcgcagtac ctgcgcatgg ccggctgggg
     1381 caccaccacg gtgatctcca gcggcaagga tgtctacatt cactatgcgg cgcccgagtt
     1441 cgccttcgcg ctggccaacg acgcgcgcag caaggcagcg gtgctctatt gcgaaccggg
     1501 cggttactac gagctcgacg cccatttcac gaaaccggtg gtggcctgcg tggtcggccg
     1561 ctggaagagc aagctgacgc gcgccgtggg tcatgccggc gcgatggcgg g
//
LOCUS       DATANS010000293         1600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_57268, whole genome shotgun sequence.
ACCESSION   DATANS010000293 DATANS010000000
VERSION     DATANS010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57268"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VHT22_04175"
     CDS             <1..288
                     /locus_tag="VHT22_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136783.1"
                     /translation="AIDPFSKQEQAVQRPVQQAGPFDLRIPRMQFADYPRTELRLFDS
                     AAHAQANAPQLVVYYLGVPDTTPEFADAAKLDASLSERIARARAPGGGTPR"
     gene            285..944
                     /gene="deoC"
                     /locus_tag="VHT22_04180"
     CDS             285..944
                     /gene="deoC"
                     /locus_tag="VHT22_04180"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX3136784.1"
                     /translation="MTRSELARMLDHSVLKPESTADDIRAGIDVVRVWRIGYFCVQPC
                     WVKLAAGALDGVDAGVVSVVGFPHGADRAETKTHAAVLAQTDGAREIDTVLNIGALKS
                     GAHAEAAADIAAVVRAVAGLPVKVIVEAAALTDDEKRLACRLAVDAGAAFVKTSTGFH
                     PAGGATVADVRLMRAVVGPQVGVKASGGIRTLADALAMIAAGANRIGTSASASILAAL
                     G"
     gene            complement(1011..>1600)
                     /locus_tag="VHT22_04185"
     CDS             complement(1011..>1600)
                     /locus_tag="VHT22_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3136785.1"
                     /translation="GMFGTTRNLKVEPYDPAGAKKLLAEAGYPDGFGITIHAPNNRYV
                     NDAKIAQAVAQMLTRIGIATKVEAMPSATYFPQATDLKFSLMLLGWSSGTGEASSPLK
                     ALLATYSRDKGFGTANRGRYSNPRVDALLEEALATVDDSKREALLQRATEIAIDDTGI
                     IPLHFQVNLWATRNGITYVPRTDENTLAHKFRPAK"
BASE COUNT          248 a          535 c          538 g          279 t
ORIGIN      
        1 gccatcgatc cgttcagcaa gcaggagcag gcagtgcagc gaccggtgca gcaggcaggg
       61 ccgttcgatc ttcgcatccc acgcatgcaa ttcgcagact atccgcgcac cgaactccgc
      121 ttgttcgact ccgcggcgca cgcgcaagcg aacgcaccgc agcttgttgt ctactatctc
      181 ggcgtccccg acacgacgcc ggaattcgcc gacgccgcaa agctggacgc gtcgctatcg
      241 gaacgcatcg cgcgggcccg agcccccggc ggaggcactc cgcggtgacc cgcagcgaat
      301 tggcgcgcat gctcgatcat tcggtgttga agccggaatc gaccgcggac gacattcgcg
      361 ccggcatcga cgtcgttcgc gtctggcgaa tcgggtattt ctgcgtgcag ccgtgctggg
      421 tgaagcttgc cgccggcgca ctggacggcg tcgatgcggg cgttgtcagc gtcgtcggct
      481 ttccgcacgg cgccgacagg gcagaaacca agacacacgc cgccgttctc gcccagaccg
      541 acggagcccg cgaaatcgac accgtgctca atatcggagc gctgaagagc ggcgcccacg
      601 ccgaggccgc cgcggatatc gccgccgtgg tccgcgcagt ggcgggcctg ccagtcaagg
      661 tgatcgtcga agccgcagcg ctgaccgacg acgagaagcg gctcgcgtgc cggctcgccg
      721 tcgacgccgg cgccgcgttc gtcaagacgt caaccggctt tcaccctgcc ggaggcgcga
      781 cggtcgccga cgtgcggctg atgcgcgccg tcgtcgggcc gcaggtcggc gtcaaggcgt
      841 cgggcggaat tcgcacgctt gccgatgcgc tcgcgatgat cgccgcgggc gcgaatcgca
      901 ttgggacgtc ggccagcgcg tcgattctcg ccgcacttgg ctgaccggcg cgcaactgcg
      961 tcgcctcgca ttgggggcga gttgcgcctt cgatgccctc gcgggcagcg tcacttcgcc
     1021 ggacggaact tgtgcgccag cgtgttctcg tcggtgcgcg gcacgtaagt gatgccgttg
     1081 cgcgtcgccc agaggtttac ctggaaatgc agcgggatga tgccggtgtc gtcgatcgcg
     1141 atctcggtcg cgcgctgcag caaagcctcg cgcttggaat cgtcgacggt tgccagtgcc
     1201 tcctcgagca gcgcatcgac tctcggattc gagtagcgac cgcggttcgc cgtcccgaag
     1261 cccttgtcgc ggctgtaggt cgccagcagc gctttcagcg ggctcgacgc ttcgccggtg
     1321 cctgaactcc agccgagcag catcaagctg aacttgagat cggtggcctg cggaaagtag
     1381 gttgccgacg gcatcgcctc gaccttggtc gcgatgccga tccgggtcag catctgggcg
     1441 accgcctgcg cgatcttggc gtcgttgaca tagcgattgt tcggcgcgtg aatcgttatg
     1501 ccaaagccgt ccgggtagcc ggcctcggcg agcagctttt tcgcgccggc cggatcgtac
     1561 ggctcgacct tcaggttcct ggtcgtgccg aacatgccgt
//
LOCUS       DATANS010000294         2577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_811226, whole genome shotgun sequence.
ACCESSION   DATANS010000294 DATANS010000000
VERSION     DATANS010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811226"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="VHT22_04190"
     CDS             <1..1031
                     /locus_tag="VHT22_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HEX3136786.1"
                     /translation="SLDVRKKMLSVDGDVAALTDVMRVWLTRHKRWGSPVYLAGESYG
                     TTRGAALADKLQSAGVSLSGLILISCAMDLQSIVFCPANDLPYSLFLPAFANVSQYHG
                     LLKGSIAKTPAAARAAAEAFVNEDYVAALQAGARLDGKGRSRIARRIAELTGLPQALV
                     EEKNLRISEMTYFFEALRPQGRIVGRLDARATGPMAASRTREWEFDPGIEAIASPYTM
                     AAMAYFGEQLGMTSELRYEVLATDVHKGWDWNRGEAKGNSFATTSRDLARALRRNPHL
                     KVFVGSGRYDLGTPYSATDWSLAQLDAPADVLTRVQHHYYDAGHMMYTREEDMTQLKA
                     DIAAWLGT"
     gene            complement(1051..2505)
                     /locus_tag="VHT22_04195"
     CDS             complement(1051..2505)
                     /locus_tag="VHT22_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3136787.1"
                     /translation="MERTARQVGRIVESSIPARLDALPWSRFHTLVIVALGITWILDG
                     LEVTLAGAVSGALKASPSLRLSDAEVGLSASAYLTGAVLGALLFGWLTDRFGRKRLFF
                     ITLGVYVLATAATAFSENFATFALFRFMTGAGIGGEYSAINSAIQELIPARYRGRTDL
                     GINGSFWVGAALGAIGSSLLLQPGRLPPDVGWRLAFGLGAILGLGILFLRRYLPESPR
                     WLLLHGRTEEAERIAVAVEREVRGRATASELLPRLRLRIRARAVSFADLGVTLFRHYP
                     RRAVLGLVLMTAQAFCYNAIFFTYALVLGRFYAVEPQDVGLYLLPFAVSNFLGPLVLG
                     RYFDIWGRRQMISTTYALSGLLLGLTAILFVAGLLNAATQTLAWTLVFFFASAAASSA
                     YLVVSESFPVEVRALSIAFFYALGTAIGGIGAPWLFGALIGTGEPAAIAWGYALGAIL
                     MLSAAAVAMWLGVAAERKPLEEVARPLSSVDFAG"
BASE COUNT          523 a          829 c          827 g          398 t
ORIGIN      
        1 ccagtctcga cgtgcgcaaa aaaatgctct cggtcgacgg agacgtcgcc gcgctgacag
       61 acgtgatgcg ggtctggctg acgcggcaca agcgctgggg ctcgcccgtc tacctggccg
      121 gggagagcta cggcaccaca cggggcgcgg cgctcgccga caagctgcaa agtgccgggg
      181 tctcgctctc gggtctgatc ctgatctcgt gcgcgatgga tctgcagagc atcgtcttct
      241 gtcctgctaa cgatctgccg tattcgcttt tcctgcccgc gttcgccaac gtctcgcagt
      301 atcacgggct gctgaagggc tcgattgcca agacgcccgc cgccgcgcgt gccgcggccg
      361 aagcttttgt gaacgaggac tatgtcgccg cgcttcaggc tggcgcccgc ctggatggca
      421 agggacgcag ccgcatcgcg cgccgcatcg cagagctgac gggactgccg caggcgctgg
      481 tcgaggaaaa gaacctgcgc atcagcgaaa tgacttactt cttcgaggcg ctccgtccgc
      541 aaggccgtat cgtcggccgc ctcgatgctc gcgccaccgg gccgatggcc gccagccgca
      601 cgcgtgagtg ggagttcgat ccgggcatcg aagccattgc atcgccgtac acgatggcgg
      661 cgatggccta tttcggtgag cagctcggca tgacctccga gctgcgctac gaggtgctgg
      721 cgacggacgt gcacaaaggc tgggattgga atcgcggcga agccaagggc aacagtttcg
      781 cgacgaccag ccgcgatttg gcgcgcgcgc ttcgccgcaa cccgcatctg aaggtgtttg
      841 tcggcagcgg acgttacgac ctcgggacgc cgtacagtgc gaccgactgg tcgctggcgc
      901 aactcgacgc accggccgac gtgctgaccc gcgtccagca ccactactac gacgccggtc
      961 acatgatgta cacgcgggaa gaggacatga cgcagctcaa ggctgatatc gcggcatggc
     1021 tgggaacttg agtcgaccgc ggcgctgtac ctagccggcg aaatccacgc tcgatagcgg
     1081 gcgcgcaact tcctcgagcg gcttccgctc ggctgcgact ccgagccaca tggcgacggc
     1141 agccgcgctg agcatcagaa ttgcccccaa cgcgtatccc cacgcgatcg cggcgggctc
     1201 gcccgtcccg atgagcgctc cgaacaacca gggggcgccg attccgccga tcgcggtgcc
     1261 caatgcgtag aagaatgcga tggacaaggc tcgcacctcc accggaaagc tttcgctgac
     1321 cacgaggtag gccgagctcg cagccgcgga cgcgaagaag aacacgaggg tccacgccag
     1381 cgtctgcgtg gctgcgttca acaagccggc aacgaacaga atggccgtca ggccgagcag
     1441 caatcccgaa agcgcatagg tcgtgctgat catctgccgg cggccccaga tatcgaagta
     1501 gcgaccgaga accagaggac cgaggaaatt gctcacggcg aatggcaaca gatagagtcc
     1561 cacgtcctga ggctccaccg catagaagcg acccagcacg agcgcatagg tgaagaaaat
     1621 cgcgttgtag caaaaggcct gcgccgtcat gagcactaaa ccgagcaccg ccctgcgcgg
     1681 ataatgccga aaaagcgtga cgccgagatc cgcgaagctg acggcgcgcg cgcggatccg
     1741 caggcgcagc cggggcagca attccgacgc ggtggcgcgg ccgcgcactt cgcgttccac
     1801 cgcgacggcg atgcgttccg cttcttcggt ccgcccgtgc agcaacagcc aacgcggact
     1861 ttccggcaga tatcgcctca gaaaaaggat gccgagaccg agtatcgccc ccaatccgaa
     1921 tgccaatctc cagccgacgt cgggaggaag gcgtccgggc tggagcaaca ggctggagcc
     1981 gatcgcgccc agtgcggcgc ccacccagaa gcttccattg atgccgagat cggtgcgacc
     2041 gcgatagcgc gccggtatca gttcctggat cgcggaattg atcgctgagt actcgccgcc
     2101 gattccggca ccggtcatga agcgaaaaag cgcaaatgtg gcgaaatttt cggaaaaggc
     2161 cgtagccgcc gtcgcaagga cataaacccc gagcgttatg aagaaaaggc gcttgcgtcc
     2221 gaaccggtcg gtgagccagc cgaacaggag cgcgcccagc acggcgccgg tgagataagc
     2281 gctggccgac aatccgacct cggcgtcgct gagtcgcaat gaggggctgg ccttgagcgc
     2341 tcccgacaca gcgccggcga gcgttacttc gaggccgtcg agtatccagg tgatcccgag
     2401 cgccacgatc accagcgtat gaaagcgcga ccagggcagg gcgtccagcc gcgcggggat
     2461 ggagctctcg actattcttc cgacttgtcg tgcagttcgt tccatcgggc gaatgacagc
     2521 gtactacatc gaccgccggg ccgactttct tcatccaggt cagatcggga tagctga
//
LOCUS       DATANS010000295         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_184459, whole genome shotgun sequence.
ACCESSION   DATANS010000295 DATANS010000000
VERSION     DATANS010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184459"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1574)
                     /locus_tag="VHT22_04200"
     CDS             complement(<1..1574)
                     /locus_tag="VHT22_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3136788.1"
                     /translation="MLFGFTLNTLTLFAIVLATGIVVDDAIVVLENVERLMAEKKLSP
                     KDAAVESMKEVTGAIVAIELVLVSVFVPVAFLGGLAGKLYQQFAVTVATAVTISGLIA
                     LTLTPALCALLLKPQHIENRLFRPFNRGFAWLTRTYLGGVRLTVRHAVIAILLFVGVL
                     GADALLFRTVPGGFVPPEDQGYILGSLTLPDAATLQRTADAGATIQRILRGHPAVEHV
                     FVVAGFDVIAGANKSNAATMFIPLQPWDERKTTAEDLAKYVVAEGAKLSQGTLLAFNP
                     AAIRGLGSAGGFEVYLQDRADADPQKLYQNLQQFLGELRKRQELTGIASFYRPTSPQL
                     FVDVDREKAAALGVPLKDVFDTLQGTMGVLYVNDFNKFGHTYRVQLQAEGAYRSKPED
                     LGNVYVRSSRGDMIPVKALISVKNVIGPEQIDRFNGFLAAKVLGNSAAGVSSGQAIAA
                     VEDAAKSLPAGYTIAWTGQAFQEKRIGTQSIIAFTFAIIMVFLILSANYEKWSLPVAV
                     LLAVPFALFGALTAVFL"
BASE COUNT          303 a          520 c          502 g          260 t
ORIGIN      
        1 aggaagacgg cggtcaacgc gccgaacagc gcgaagggca cggccagcag cactgccacc
       61 ggcagcgacc atttctcgta attcgccgac aggatcagga acaccatgat gatcgcgaac
      121 gtaaacgcga tgatcgactg agtgccgatg cgtttttcct ggaatgcctg cccggtccag
      181 gcaatcgtat agccggccgg aagcgatttc gcggcgtcct cgaccgcggc gatcgcctgc
      241 ccggagctta cgccggccgc gctgttgccg agcaccttgg ctgccaggaa accgttgaag
      301 cgatcaatct gctcgggtcc gatgacgttc ttcacgctga tcagcgcctt caccggaatc
      361 atgtcgccgc gcgaggaacg gacgtacacg ttgcccagat cctccggctt cgaccgatac
      421 gcaccctcgg cctggagctg cacgcgatag gtgtggccga acttgttgaa gtcgttgaca
      481 tagagcacgc ccatcgtgcc ctgcagcgtg tcgaacacgt ccttcaaggg caccccgagc
      541 gccgcggcct tttcgcgatc gacatcgaca aacagctgcg gagaagtcgg gcggtagaac
      601 gaagcgatgc cggtaagctc ctgccgcttg cgcagctcgc cgaggaactg ctgcagattc
      661 tgatagagct tctgcggatc ggcgtcggcg cggtcctgca ggtagacctc gaacccgccg
      721 gcgctaccga ggccgcgtat ggccgcgggg ttgaatgcca gcagcgtgcc ctgcgacagc
      781 ttggcgcctt cggccaccac gtacttcgcc aggtcctccg ccgtcgtctt gcgctcgtcc
      841 cacggctgca gcggtatgaa catcgtcgcg gcgttgctct tgttggcgcc ggcgatgacg
      901 tcgaagccgg cgacgacgaa tacgtgctcg accgctggat ggccgcgcag aatccgctgt
      961 atcgtggcgc cggcatcggc ggtgcgctgc agcgtcgcgg catcgggcag tgtcagcgag
     1021 ccaaggatgt aaccctggtc ttccggcggc acgaagccgc ccggcacggt tcggaacagc
     1081 agcgcatccg cgccgaggac gccgacgaac agcaggatcg cgatgaccgc atgacgcacg
     1141 gtcagccgca cgccgccgag ataagtgcgg gtcagccagg cgaacccgcg gttgaacggg
     1201 cggaacaggc ggttttcgat gtgctggggc ttgagcagca acgcgcacag cgccggcgtc
     1261 aatgtcaacg cgatcagtcc cgaaatcgtc accgcggtgg ccaccgtcac ggcgaattgc
     1321 tggtagagct tgccggcaag gccgcccagg aacgcgaccg gcacgaacac cgacaccagc
     1381 accagctcga tcgcaacgat cgcgccggtc acctctttca tcgattcgac cgccgcgtcc
     1441 ttcggcgaga gcttcttttc ggccatcagc cgctcgacgt tttccagcac gacgatagcg
     1501 tcgtcgacga cgatacccgt cgcaagcacg atcgcgaaca gggtcagcgt gttcagcgta
     1561 aagccgaaga gcatcaggcc gccga
//
LOCUS       DATANS010000296         2305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_811454, whole genome shotgun sequence.
ACCESSION   DATANS010000296 DATANS010000000
VERSION     DATANS010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811454"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1490
                     /locus_tag="VHT22_04205"
     CDS             <1..1490
                     /locus_tag="VHT22_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3136789.1"
                     /translation="SVTALSILRLVQQPGRIEPGSRIMFENQDLLQLDDAGIRRVRGN
                     RIAMIFQEPMTALNPVMRCGEQIDEVLATHTPMDAAERRARVAAIIRDVHLPDADRMI
                     TSFPHQLSGGQRQRIMIAMALVLNPKLLIADEPTTALDVTTQAQILALIREMQRMHGT
                     GVLFITHDFGVVAEIADRVAVLRRGELVEFAPVDELLAHPQHEYTRMLIAAVPGVAPP
                     SRETRIGPTVLATEGLSKTYARGGWLKRRRDDVAAAVDVTLEIRRGETLGIVGESGSG
                     KSTVARCIARLIEPSAGRIVLDGEDIARLDGDALRPRRRRVQIVFQDPYRSLNPRRTV
                     GDAIVEGPMNYGATRADALKTATELLALVRLDARALDRYPHQFSGGQRQRICIARALA
                     IGPDLLIADEAVSALDVSVQAQVLALLDEIRLRLDLAMLFITHDLRVAARVCDRVAVM
                     QQGRIVEQGPVGEVFGAPRHEYTKALFAAVPGRRWSFASDAARVA"
     gene            complement(1487..>2305)
                     /locus_tag="VHT22_04210"
     CDS             complement(1487..>2305)
                     /locus_tag="VHT22_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3136790.1"
                     /translation="RRAVKLHRQIQPSLSFVFFNMDDPVVGGYTPEKIALRRAMVMGY
                     DRRAEIDVLRHGQAEPASQLVPPGLAGHDPTLRANDLYDPAAARALLDKFGYKDRDGD
                     GYRETPDGKPLQITKANTPSAIDRVDNELWKKCMDAIGIRIVFFTQKWPELNKMSEAG
                     QLQMWGLSWISNAPDGQAFASPLYSKNIGTSNDARFRLPAYDRAYEASLNLPDGPART
                     ALFRSMSEAVLNYAPWMMDLNVYVSVLAQPWLRGYKQNPFLRNQWKYYDVDKRR"
BASE COUNT          385 a          767 c          747 g          406 t
ORIGIN      
        1 agtcggtcac cgcgctttcg attctccgac tcgtccagca gccgggtcgc atcgagcccg
       61 gaagccgcat catgttcgag aaccaggatc tcctccagct cgacgatgcc ggcatccgtc
      121 gcgttcgcgg caatcgcatc gcgatgatct tccaggaacc gatgacggcg ctcaatccgg
      181 tgatgcgttg cggcgagcag atcgacgagg tccttgcgac gcatacgccg atggacgcgg
      241 ccgagcggcg agcgcgcgtg gcggcgatca tccgcgacgt ccatctgccc gacgccgatc
      301 ggatgataac ctcgtttccg catcaactat ccggcggtca gcgccagcgc atcatgatag
      361 cgatggcgct ggtgctcaat cccaagcttc tgatcgccga cgagccgacg actgcgctgg
      421 atgtcacgac gcaggcgcag atcctcgcgc tgatccggga aatgcagcgc atgcatggca
      481 ccggcgtgct gttcatcacg catgatttcg gcgtcgtcgc ggaaatcgcc gatcgagtcg
      541 cggtgctgag gcgaggcgag ctggtcgagt tcgcgccggt cgacgagctg ctcgcgcatc
      601 cgcagcacga atacacgcgc atgctgatcg cggccgttcc cggcgtcgcg ccgccgtcac
      661 gcgaaactcg gatcggcccc actgtcctgg cgaccgaggg gctttccaag acctacgcgc
      721 gaggcggatg gctgaagcgc cggcgcgacg atgtcgccgc cgccgtcgac gtgacgctcg
      781 agatccggcg cggtgagact ctgggcatcg tcggagagtc gggttcgggc aaatctacgg
      841 tcgcgcgctg catcgctcgg ctgatcgagc cgtcagccgg gcgcatcgtc ctcgacggag
      901 aggacatcgc acggctcgat ggcgacgcgc ttcgcccgcg gcgccgccgc gtccagatcg
      961 tgttccagga tccttaccga tcgctcaatc cgcgccgcac cgtcggcgac gcgatcgtcg
     1021 aggggccgat gaactacggc gcgacgcgcg ccgatgcgct gaagaccgcg accgagctgc
     1081 tggccctggt ccggctcgac gcgcgtgcgc tcgatcgcta tccgcaccag ttctcgggcg
     1141 gacagcgcca gcgcatctgc attgcacgcg cactggcgat cgggcccgac ctcttgatcg
     1201 ccgacgaagc ggtctccgcc ctcgacgtct cggtgcaggc gcaagtgctg gcgctgctcg
     1261 acgagatcag gctgcgactc gatctcgcga tgctattcat cacccacgat ctgcgcgtcg
     1321 cggcgcgggt ctgcgatcgg gtcgcggtga tgcagcaggg gcgcatcgtc gagcaagggc
     1381 ccgtcggaga agtcttcggc gcgccgcggc acgagtacac gaaggcgctg ttcgccgccg
     1441 tccccggcag gcgctggtcc ttcgccagcg acgctgcacg agttgcctag cggcgcttgt
     1501 ccacgtcgta atacttccac tgattgcgca ggaacggatt ctgcttgtaa cccctgagcc
     1561 acggctgcgc cagcacgctg acgtagacgt tcagatccat catccacggc gcgtagttca
     1621 gcaccgcctc gctcatcgag cggaagagcg ccgttcgcgc cggcccatcc ggaagattga
     1681 gcgacgcctc gtaggcgcga tcgtaggcgg gcaagcgaaa gcgcgcatcg ttcgacgtgc
     1741 cgatgttctt gctgtacagc gggctcgcga acgcctggcc gtccggcgca ttcgatatcc
     1801 acgacagccc ccacatctgc agctggccgg cttccgacat cttgttgagt tccggccact
     1861 tctgcgtgaa aaacacgatg cgaatcccga tcgcgtccat gcattttttc cacagctcgt
     1921 tgtcgacgcg gtcgatcgcc gacggcgtgt tggccttggt gatttgcaac ggcttgccat
     1981 cgggcgtctc gcgataacca tcgccgtcgc ggtccttgta gccgaacttg tcgagcagcg
     2041 cgcgggcggc ggccggatca tacaggtcat tggcgcgcag cgtcggatcg tgaccggcga
     2101 gccccggagg gacgagctgc gacgcaggct ccgcctggcc atggcgcaag acgtcgattt
     2161 cagccctgcg gtcgtagccc atgaccattg cgcggcgcag tgcgatcttc tccggcgtat
     2221 atccgccaac gaccggatcg tccatattga aaaagacaaa actgagcgaa ggctggatct
     2281 ggcgatgtag cttgacggcg cggcg
//
LOCUS       DATANS010000297         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_436681, whole genome shotgun sequence.
ACCESSION   DATANS010000297 DATANS010000000
VERSION     DATANS010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436681"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1531
                     /locus_tag="VHT22_04215"
     CDS             <1..>1531
                     /locus_tag="VHT22_04215"
                     /inference="COORDINATES: protein motif:HMM:NF015926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HEX3136791.1"
                     /translation="ANVEALVVEAERLAAGTTVDDAQLPVRWQALDRKLRTPALTQRF
                     ETALIIVEQRRLAFLETTRQQTQAVRQQVHNLLHAAEQALAAGQLQTARTAADNVKKM
                     KLAAGLLPKPTMQRLGRLMHQLVELERWESFGQQNARVQLCERAEALAAQTLDAPQLA
                     AEVQKLRNEWKALDQQHAGVPKALWERFDGACEKAYAPAARHFAEQAALRKQGRKQRE
                     EFIATAAAHAPTLLGESDVGRDWRAIERWLRETEQKWREGELGSVEPRAWKKLDSELK
                     AAVAPLRDALSTAREQAKAARVALIEEATGLAAKAMERDTLTHVKALQAKWQEQAKAI
                     SLLQRDERPLWEQFRAACDAVFAARDAKRKEQVDIKQQGRRELEELCVQLEALAAATG
                     KGDQEIRQAARAAAEQWRKLRGGHDPALRGIETRFKKAQTAVDAMLAERARAQKAAVW
                     TTLAAKVQVCEELDGLVRAGNAAPDSGTAWQDKWAALAALPDAWEKKMLARRDGALSS
                     LA"
BASE COUNT          317 a          497 c          530 g          187 t
ORIGIN      
        1 cgcgaacgtc gaggcgctcg tcgtcgaagc cgagcggctc gccgccggca cgacggtaga
       61 cgacgcgcaa ttgccggtgc gctggcaggc gctggaccgc aagctgcgca cgccggcgct
      121 cacgcagaga ttcgaaacgg cgctgatcat tgtcgagcag cggcggctcg cgtttctgga
      181 aaccacgcgc cagcagacgc aagccgtgcg ccagcaggtc cataatctgc tgcacgccgc
      241 cgagcaggcg ctcgccgccg gccaattgca aacggcgcgc accgccgcgg acaacgtcaa
      301 aaaaatgaag cttgctgccg gcctgctgcc caagccgacc atgcagcggc tgggccggct
      361 catgcaccag ctcgtcgagc tcgaacgctg ggaatcgttc ggccagcaaa acgcgcgcgt
      421 tcagttatgc gaacgcgccg aggcgctcgc cgcgcaaacg ctggatgcgc cgcaactcgc
      481 cgccgaagtg caaaagctgc gcaacgaatg gaaggcgctc gaccagcagc atgcaggcgt
      541 gcccaaagcg ctgtgggagc gcttcgacgg tgcctgcgaa aaagcgtatg cgccggccgc
      601 gcgccatttc gcggaacagg cggcattgcg caaacagggc cgcaaacaac gcgaagaatt
      661 catcgccaca gccgcggccc acgcgccaac gctgctgggc gagtcagacg tggggcgcga
      721 ctggcgcgcg atcgagcggt ggctgcgcga gaccgagcag aaatggcgtg aaggcgagct
      781 cggcagcgtc gaaccgcggg catggaagaa gctcgactcc gaactgaaag cggcagtcgc
      841 gccgttgcgc gatgcgttgt cgacggcgcg cgagcaagcc aaagccgcgc gtgttgcgct
      901 gatcgaggaa gccacgggtc tcgccgccaa ggcaatggag cgcgatactc tcactcacgt
      961 gaaggcgctg caggccaaat ggcaggagca ggccaaagcg atctcgctgc tgcagcgcga
     1021 cgaacgtccg ctatgggagc aattccgcgc cgcctgcgac gccgtcttcg cggcccgcga
     1081 tgcgaaacgc aaagagcagg tggacataaa acaacagggc cggcgcgaac tcgaggaact
     1141 ctgcgtgcag ctcgaggcgc tcgccgcggc gaccggcaaa ggcgatcagg aaatccgcca
     1201 ggcagcgcgc gccgcggccg agcaatggcg caagctgcgc ggcggccacg atccagcttt
     1261 gcgcggcatc gaaacgcgct tcaaaaaagc gcagaccgcc gtcgatgcga tgctagcgga
     1321 acgcgcccgc gcgcagaagg cggccgtgtg gacgacgctc gccgcgaaag tgcaggtgtg
     1381 cgaagaattg gacggcctcg tgcgcgcggg caacgctgcg ccggattcag gtaccgcttg
     1441 gcaggacaaa tgggctgcac tggctgcgct gcccgacgca tgggagaaga aaatgctggc
     1501 gcgccgcgat ggcgcgttga gctccctcgc c
//
LOCUS       DATANS010000298         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_811514, whole genome shotgun sequence.
ACCESSION   DATANS010000298 DATANS010000000
VERSION     DATANS010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811514"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VHT22_04220"
     CDS             <1..597
                     /locus_tag="VHT22_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase
                     kinase/phosphatase-domain containing protein"
                     /protein_id="HEX3136792.1"
                     /translation="DAVAPSLIEEDGDMIVVRHLYIERRMNPLNLFLESASPRQREHA
                     IADYGEAIRQLAAVNIFPGDLLFKNFGVTRFGRIVFYDYDEIEYMNSCRFRAIPQPPA
                     AHDEMSDEVWYPVGAHDVFPEEFATFLLTDPEVRTDFLRVNADLLDAQWWQRMQQEAG
                     ELPPEVLSYPEPLRFAHVSAERPPAASTPVGALEPPLR"
     gene            complement(594..1259)
                     /locus_tag="VHT22_04225"
     CDS             complement(594..1259)
                     /locus_tag="VHT22_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3136793.1"
                     /translation="MTSGFMRLSRHALVVTILAAATASIAAADTRGWLAYQRGDFAAA
                     RAAYEHDAQLGDRLAQFNLATMMLRGEGGAADPEKGVDWLRKAAEAGMAQAQYSLGLL
                     YESGTGVARSLTTATEWWERAAQQGHVDAQVQLGTQYFLGRGAPKDWTLAAKWYEAAA
                     ENGDAGAQYLIASFYEHGDGVAPDLKRALGWYLQAARQGDAGAALQASDVARRLGAAP
                     PAR"
     gene            1318..>1520
                     /locus_tag="VHT22_04230"
     CDS             1318..>1520
                     /locus_tag="VHT22_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016804803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase beta chain"
                     /protein_id="HEX3136794.1"
                     /translation="MFVSAFDLFKIGLGPSSSHTMGPMRAAYRFVHELAADQPLEAVT
                     QIFVDLYGSLALTGRGHGTDRAIL"
BASE COUNT          237 a          538 c          473 g          272 t
ORIGIN      
        1 gacgccgtcg ccccgtcgct gatcgaggag gatggcgaca tgatcgtcgt gcgccatctg
       61 tacatcgagc ggcggatgaa cccgctgaac ctgttcctcg aatccgcctc cccgcgccag
      121 cgtgagcacg cgattgccga ctacggcgag gcgatccggc agctggccgc ggtcaatatc
      181 ttccccggcg acctgctgtt caagaatttc ggggtcacgc gcttcggccg catcgtcttc
      241 tacgactacg acgagatcga gtacatgaac tcgtgccgct ttcgcgcgat tccacagccc
      301 ccggccgcgc acgacgaaat gtcggacgag gtatggtatc cggtcggtgc gcacgatgtt
      361 tttccggagg agttcgcgac ctttctgctg accgaccccg aagtgcggac tgattttctg
      421 cgcgtcaacg cggatctgct ggatgcgcaa tggtggcagc ggatgcagca agaggccggc
      481 gaactgccac cggaagtgtt gtcgtatccc gagccgctcc gctttgcaca cgtcagcgcc
      541 gagcgtccgc cggcggcttc gacaccggtc ggcgcactgg aaccgccgct gcgctagcgc
      601 gccggcggcg cggcaccgag ccggcgcgcg acatcgctgg cctgcaaggc cgcaccggcg
      661 tcgccctgtc gtgccgcctg caggtaccag cccagcgcac gcttcaagtc gggcgcgacg
      721 ccatcgccgt gctcgtagaa gcttgcgatc aggtattgcg cgccggcgtc gccgttctcg
      781 gcggcggctt cgtaccactt ggcggcaagc gtccaatcct tcggcgcgcc gcgcccgaga
      841 aagtactgtg tgccgagctg gacctgcgcg tcgacgtgtc cctgctgcgc agccctttcc
      901 caccactcgg tcgccgtggt cagcgagcgc gcgaccccgg tcccgctttc atagagcagg
      961 ccgaggctgt actgggcctg cgccatgccg gcctccgcgg ccttgcgcag ccagtcgacg
     1021 cccttttccg gatcggcggc gccgccttcg ccgcgcaaca tcatcgtcgc caggttgaat
     1081 tgcgcgagcc gatcgcccag ctgcgcgtca tgctcgtagg ccgcgcgcgc ggccgcaaaa
     1141 tcaccgcgct gataagcgag ccagccgcgg gtgtccgccg ccgcgatcga cgcagtcgcg
     1201 gcggccagta tcgtgacgac cagcgcatgt cgcgataagc gcatgaaccc ggatgtcatc
     1261 ggctctctcc ggagtacaat tacacagtcc agtctaccac ccagcggatc tcgttccttg
     1321 ttcgtcagcg cattcgattt gttcaagatc ggtctcgggc catcgagctc gcacacaatg
     1381 ggaccgatgc gtgccgcata ccggtttgtc cacgagctcg ccgccgacca gccgctcgag
     1441 gccgtcacgc agatcttcgt cgacctttac ggctcgcttg ccctgaccgg ccgcggccac
     1501 ggaaccgatc gcgcgatcct
//
LOCUS       DATANS010000299         1933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_14811, whole genome shotgun sequence.
ACCESSION   DATANS010000299 DATANS010000000
VERSION     DATANS010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14811"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..160
                     /locus_tag="VHT22_04235"
     CDS             <1..160
                     /locus_tag="VHT22_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HEX3136795.1"
                     /translation="PAVAALGVGHLKALVFVAAMVAGMLAFEWIEGLRARSTSTDRLE
                     PGAGGTSE"
     gene            164..1234
                     /locus_tag="VHT22_04240"
     CDS             164..1234
                     /locus_tag="VHT22_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3136796.1"
                     /translation="MIFRQLFDPQSSTYSYLLADRRSREAVLIDPVFEQAQRDAALIQ
                     ELDLKLVATLDTHVHADHVTGAWLFKQRCGSAIALGAASGAAGADRYLAHGDRVQFGG
                     HYLEVRATPGHTEGCVTYVMDDESMAFTGDCLLIRGSGRTDFQHGDARAMYRSVRSQI
                     LTLPPSCLLYPAHDYRGLTVTSVEEERRYNPRLGGDVGEGDFVGYMNNLGLPHPKLMD
                     IAVPANLRCGQPEGTVPLPASPSWAPLTYTFAGIFEIQPQALEEGAASIQIVDVRERD
                     EFDGPLGRIRGAKWIPLGELPARTAELARDRPVVAVCRSGARSAQASVLLSKGGFADV
                     ANLAGGMLRWRAEGHPVEGQRD"
     gene            1402..>1933
                     /locus_tag="VHT22_04245"
     CDS             1402..>1933
                     /locus_tag="VHT22_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3136797.1"
                     /translation="MEYALAAYQRGDRVEAERLCRLLLSGMPDYFDALFLAGIMAEQA
                     GKAERAVELLSKAVAVRPDVAEAHYNYGVALGDLRQYEEAVASYERAIALKHDYADAY
                     FNRGVALAELGRPVQAIESYDRAIAFNPGYAEVHNNRGIALDHLKRYEESLANYDRAI
                     ALKPDYARAHNNRGVAL"
BASE COUNT          329 a          639 c          624 g          341 t
ORIGIN      
        1 ccccgcggtc gccgcgctcg gcgtcggtca tctgaaggcg ctcgtgttcg tcgcagcgat
       61 ggtggccgga atgcttgcct tcgaatggat cgagggcctg cgggcacgct cgacctcgac
      121 cgaccgcctg gaaccgggcg ccggcggcac gagcgaatag atgctgatct tccgccagct
      181 tttcgatccg caatcgtcga cgtactcgta tctgctcgcc gatcgccgca gccgcgaggc
      241 agtcctgatc gatccggtct tcgagcaggc gcagcgcgac gccgcgttga tccaggagct
      301 cgacctcaaa ctcgttgcga cgctcgacac gcatgtgcac gccgatcacg ttaccggggc
      361 gtggcttttt aagcagcgct gcgggagcgc gatcgccctc ggtgcggcga gcggtgctgc
      421 gggggccgac cggtatctcg cgcacggcga ccgcgtccag ttcggtggcc attacctgga
      481 ggttcgcgcc acgccgggtc acacggaagg gtgcgtcacc tatgtaatgg acgatgaaag
      541 catggccttc accggcgact gcctgctcat tcgcggcagc ggacgcaccg acttccagca
      601 cggcgacgcg cgcgcgatgt accgctcggt gcgcagtcag atactgacgc tgccaccgtc
      661 gtgcctgctc tatcccgccc acgattaccg cggcttgacg gtgaccagtg tcgaggagga
      721 gcgccgctac aatccgcggc tcggcggcga tgtcggcgaa ggcgacttcg tcggctacat
      781 gaacaatctg gggctgccgc acccgaaatt gatggacata gccgtcccgg cgaacctgcg
      841 ttgcggacag cccgagggca cggttccatt gcccgcgtct ccgtcatggg cgccgctgac
      901 ctacacgttc gcaggcattt tcgaaataca gccgcaggct ctcgaagaag gcgcggcttc
      961 gatccagatt gtcgacgtgc gcgagcgcga tgaattcgac ggaccgctcg gccggattcg
     1021 cggggcgaaa tggattccgc tcggcgagct gccggcgcgc accgccgagc tggcccgcga
     1081 tcgtccggtc gtggccgtct gccgctccgg tgcgcgctcc gcgcaggctt cggtgcttct
     1141 ttccaagggc ggcttcgccg atgtcgccaa cctcgccggc ggcatgctgc gctggcgcgc
     1201 cgaagggcac ccggtcgaag gccagcgcga ttagccgcgc gcgacgcgtg ctatcgtatc
     1261 gccacccgaa tattccgcct gatcgctgcc gccggcgcca atggcggcca ctgcataccg
     1321 cgagctaatt tcgccatggc ccccgttcct ccatcagcgc ctacgcctcg gcgcgccgta
     1381 accataagtt ttccggaggc gatggaatac gctttggctg cgtatcaacg cggcgatcgc
     1441 gtcgaagcgg agcgcctgtg tcgactgctg ctgagcggca tgccggacta tttcgacgcg
     1501 ctgttcctgg caggaatcat ggccgagcag gccggaaaag cggaacgcgc cgtcgaactg
     1561 ctgtcgaagg ccgtagctgt caggcccgat gtcgccgagg ctcactacaa ctacggcgtg
     1621 gcactgggcg atctccgcca atacgaagaa gcggtggcga gctacgagcg cgcgattgcg
     1681 ctgaagcacg attatgccga cgcctatttc aaccgtggcg ttgcgctggc cgagctcggc
     1741 cgtccagtgc aggcaataga gagctatgac cgcgcgattg cattcaaccc cggatacgcc
     1801 gaagtccaca ataatcgcgg catcgcgctg gatcatctga agcgttatga ggagtccctg
     1861 gcgaactacg atcgtgccat tgcgctcaaa cccgattacg caagggccca caacaatcgc
     1921 ggcgtcgcgc tgg
//
LOCUS       DATANS010000300         3329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_730144, whole genome shotgun sequence.
ACCESSION   DATANS010000300 DATANS010000000
VERSION     DATANS010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730144"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..740
                     /locus_tag="VHT22_04250"
     CDS             9..740
                     /locus_tag="VHT22_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEX3136798.1"
                     /translation="MDDTLSTRGRITAQAYAAMESLRAAGLLLIPITGRPAGWCDHIA
                     RMWPVDAVVGENGALYMLRDQGAGRLLKRYVATDAERAANRLRLAEIAEAILREVPGA
                     ALASDQPYRESDLAIDFCEDVPRLPDDAIDRIIAMMRAAGMTAKMSSIHVNGWFGAYD
                     KLAMTRTLLRDEFGIDVDAERDRFIFVGDSPNDAPMFQHFPYSVGVANVRAFVGRIAT
                     PPVYVTERESGSGFAELAAHLLAAR"
     gene            1094..2212
                     /locus_tag="VHT22_04255"
     CDS             1094..2212
                     /locus_tag="VHT22_04255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX3136799.1"
                     /translation="MKLRDVAGAVLASVAMTLGMTGQSGALTLNVFTDPHPPMCCGTI
                     GFAYIGDGFVGSVQADGTGDVLYRTDLTGGNVQIFAPTVAIASNPSSEHFVASSLGLG
                     GFPDRDVYVASGAGIVHIDHAGTTGSPLVTSVDGVPGSVLGAPVRGILFDAIGTFGND
                     MLVTTTSGQIYRIDSTGAATLLASVGEDTEGLDIAPTGAFGPYSGQLIVASEGSGLLR
                     AITTTGTITVINPNSPIAGAEELTFVPLNLGASGNPVEGFYGANYTPNVVKGDAGEFS
                     LMKGDIIVTGEFTHLVWNVHWDTTLLAPVNSVLGSFPNQPEDGIFVTAAIINPCPPGE
                     VCGPRSVPEPDSLLLLAAALLACAAGASVSRRRKFRAD"
     gene            complement(2407..>3329)
                     /locus_tag="VHT22_04260"
     CDS             complement(2407..>3329)
                     /locus_tag="VHT22_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HEX3136800.1"
                     /translation="WVAGCLQLVPALGVLALLAGCYGSYLLYLGLPVLMRCPKEKSVG
                     YTVVLILCAIVVSVIIGMLSTCAATGLGLAGIGAMGGIDRANTTATSDAGGWIAGIFG
                     GKSDADKARVSEALSQLQKIGEDAQKAGTRSAPDPKASADMTAALNAVGAIVSGGKDV
                     EPVDFRKLREMLPDTLAGMKRVDATGQSGEAMGIKGSSATARYTDGAGASLNVDISDM
                     GSLSGLAGLASRFDPSMEKETETSYERTSKVNGQIVHERYDRRAKSGEISIILAERFN
                     VAVRGNGVEPATLQGAIKQIDMAGIVALAK"
BASE COUNT          573 a         1083 c         1055 g          618 t
ORIGIN      
        1 tggccgatat cgacgacaca ctttcgaccc gaggcagaat taccgcgcaa gcctacgcgg
       61 cgatggaaag cctgcgcgcc gcggggctgc tgttgatccc gatcaccgga cgtccggccg
      121 gctggtgcga tcacatcgcg cgcatgtggc cggtcgacgc ggtggtcggc gaaaacggcg
      181 cgctgtacat gcttcgcgat caaggcgccg gcaggctgct caagcgctac gtcgcgaccg
      241 atgccgagcg cgccgccaac cgcctacgac ttgccgagat cgcggaagcc atcctgcgcg
      301 aggtgcccgg cgccgccttg gcgtcagacc agccgtatcg cgaaagcgat ctcgcgatcg
      361 acttctgcga agacgtgccg cgcctgccgg acgatgcgat cgaccggatc atcgcgatga
      421 tgcgcgcggc gggcatgacc gccaagatga gttcgattca cgtcaacggc tggttcggcg
      481 cgtatgacaa gctggcgatg acgcgaacct tgctgcgcga tgaattcggt atcgacgtcg
      541 atgccgagcg cgaccgtttc attttcgtcg gggattcgcc gaacgacgcg ccgatgttcc
      601 agcatttccc ctactcggtt ggcgtcgcca atgtgcgcgc gttcgtcggc cgcatcgcga
      661 cgccgccggt ctacgtgacc gagcgcgagt ccggctcggg attcgccgag ctggcagcgc
      721 atctgctcgc cgcgcgctaa cgccggcagg cgcgccttcg gggcaaccca ggtctgaacg
      781 ggagccgagg tgtcgccaaa ctttacgtac cctcgcgacc ctgcaagaag acgtgtcgta
      841 cggcccgacc cattgccttg ggggcgtagt gctcgttttg cgttttcgag gcctccgcgc
      901 accgaggcgg acagcgtcgg tgtaccttac aatccgcaca caatctcttt gtacttttcc
      961 agatttgagc ttaaaaatca aaaacttata gcgataggta cgaatgatgc tcgcgctggg
     1021 caggcccggc ggctggagga gcttgctcgc cagcgtggag gagccggaac caaaaagggc
     1081 gatggggggt tccatgaaac tgcgcgatgt ggccggcgcc gtcctggcgt cggtggcgat
     1141 gactttgggt atgaccgggc aatccggggc gctgacgctc aacgtattca ccgatccgca
     1201 cccgccgatg tgctgcggca cgatcggctt tgcctacatc ggagacggat ttgtcggcag
     1261 cgtccaggcg gacggaacgg gtgatgtgct gtatcgaacc gaccttaccg gcggcaacgt
     1321 gcagattttt gcgccaacgg tagctatcgc gtcgaacccc tcgtctgagc acttcgtcgc
     1381 gagctcgctc gggctcggcg gttttccgga tcgcgacgtc tacgttgctt cgggcgccgg
     1441 catcgttcac atcgatcatg ccggtacgac cgggtcgccg ctggttacca gcgtcgacgg
     1501 cgtgcccggg agcgtacttg gcgccccggt tcgcggcatc ctgttcgatg cgatcggaac
     1561 ctttggcaac gacatgctgg tcacgaccac cagcggccag atttatcgga tcgacagcac
     1621 cggcgccgcg actttgctcg catcggtagg cgaagatacc gaaggcctgg acatcgcacc
     1681 cacgggggcg tttggaccgt actcgggtca gctgatcgtc gcgtccgaag gcagcggatt
     1741 gctccgcgcg atcacgacta ccggcaccat caccgtgatc aatccgaaca gcccgatcgc
     1801 cggtgcggag gaattgacat tcgttccgct caatctcgga gcgagcggca acccggtcga
     1861 gggcttctat ggcgcaaact acacgcccaa tgtggtcaag ggcgacgccg gcgagttcag
     1921 cttgatgaaa ggcgacatca tcgtcaccgg cgaattcacc catctcgtct ggaacgttca
     1981 ttgggacaca acgttactgg cgcccgtaaa cagcgttctc ggatcgttcc cgaaccagcc
     2041 ggaagacggc atcttcgtga ccgcggcgat catcaatccc tgcccgccgg gcgaggtgtg
     2101 cggccccaga agcgtgcccg agcccgattc cctcttgctg ctggcggcag cgctgctggc
     2161 gtgcgccgca ggcgcgagcg tatcgcggcg caggaaattc cgggcggatt gagcggccat
     2221 ccgcttcaag ggggatacgc agtacggtca ttctctaccc gcgaggactc cttcatcccg
     2281 gaaatggaga gtccccgact cagcaggcct gctttcatgc aggcctcttt ttttgacgca
     2341 gcggcgcctg ctcgttcgtc gccggcatcg aatgcggcgc gccggagcca ggcgcgcgga
     2401 ctcggcctac ttcgcaagcg cgacgatccc agccatatcg atctgcttga tcgcgccctg
     2461 cagcgtcgcg ggttcgacgc cgttgccgcg caccgcgacg ttgaagcgct cggccaggat
     2521 gatgcttatc tcgccgcttt tcgcgcggcg atcgtaacgc tcgtgcacga tctggccgtt
     2581 gaccttgctg gtccgctcgt agctggtctc ggtttctttt tccatgctcg gatcgaatcg
     2641 ggatgcaagc cccgccagtc ccgacagcga tcccatgtcg gaaatatcga cgttcaggga
     2701 cgccccggca ccgtcggtgt agcgcgccgt ggcgctcgag cccttgatgc ccatcgcctc
     2761 gccgctctgc ccggtggcat cgacgcgctt cataccggcc agcgtgtcgg ggagcatttc
     2821 ccgaagcttg cggaaatcga ccggctcgac atccttgccg ccggagacga tcgcgccgac
     2881 ggcattcagg gcggcggtca tgtcggcgct cgccttggga tcgggcgcac tgcgagtgcc
     2941 tgccttctgg gcgtcctcgc cgatcttctg cagctgcgac aaggcctcgc tgacgcgcgc
     3001 cttgtcggcg tcggacttgc cgccgaatat gccggcaatc cagccgccgg cgtcggatgt
     3061 tgcggtggtg ttggcgcggt cgatcccgcc cattgcgccg atcccggcca ggcccagacc
     3121 ggtcgccgcg caagtgctca acattcctat gatcaccgac acgacgatcg cgcacaggat
     3181 cagcaccact gtgtagccca ccgatttttc cttcggacag cgcatcagca ccggcaagcc
     3241 aaggtagagc aggtacgagc cgtagcatcc ggccagcaga gctagaacgc ccagggcggg
     3301 aacgagctgg agacagccag ccacccatg
//
LOCUS       DATANS010000301         7563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_811523, whole genome shotgun sequence.
ACCESSION   DATANS010000301 DATANS010000000
VERSION     DATANS010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7563
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811523"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VHT22_04265"
     CDS             complement(<1..536)
                     /locus_tag="VHT22_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3136801.1"
                     /translation="MLPYAVLAGIALAIVWVLVASGLWTDILRYRKDVIYLTKQHLVL
                     VAVSGGAAIVTGVSLGIWLSRPSMARYAEATMQAVNMATSIPTLGKLALMMTFLGIGP
                     LPAIVGLWVATLLPIVRNTYAGLRAVPGHLVDAAQGMGMSPGAILRRVELPNALFVIF
                     AGIRTAMAVNVGTAPIAFL"
     gene            complement(575..1567)
                     /locus_tag="VHT22_04270"
     CDS             complement(575..1567)
                     /locus_tag="VHT22_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3136802.1"
                     /translation="MIRLESLTKIYPSAHGGAASVDRVSFEVGAGETCVLLGPSGCGK
                     TTTLRMINRLVAPTSGKVFIEGRDTSAADPVELRRTIGYVIQQIGLFPNMTVAENIGV
                     VPRLLNWDRARMRRRAEELLAMLALDPAEFLDRYPNELSGGQAQRVGVARALAADPPV
                     LLMDEPFGAVDPVNREVIQDEFLRMQKALRKTVLFVSHDIDEAVKMADRIAIFRAGRL
                     EQYAAPDELLGRPANEFVASFVGSDRTLKRLRRISVSEVVNGAGGGDHTTAPHAPAVS
                     PTDDLRRVATLFLEQGVDVLRCVDADGHFVGIVTRDAVAARLAAPAGALGASPQ"
     gene            complement(1590..2249)
                     /locus_tag="VHT22_04275"
     CDS             complement(1590..2249)
                     /locus_tag="VHT22_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011507648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3136803.1"
                     /translation="METLAFILHNLPAIGERTVEHVSIVSVAVGFAILTGVPLGIAIT
                     QSQRTADTVLYLGSIVMTIPSIALFGVLIPLLSKIGQGIGYLPAVIAAMLYAQLPIVR
                     NTYTAIRNVDPALRSAAIGMGMSTSQRLLRVEIPIALPVIIAGVRIAVVINIGVTAIA
                     AYIGAGGLGTFISRGISQTDIRQLITGALALALLAIAADYGLLWLQRRLTPKGAAERA
                     G"
     gene            2527..3588
                     /locus_tag="VHT22_04280"
     CDS             2527..3588
                     /locus_tag="VHT22_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX3136804.1"
                     /translation="MTPTATQRPSNSLTERLDQGPVICAEGYVFELERRGYLQAGAFV
                     PEVVLEHPEVVAQLHRDFVHAGSDVVEALTYYAHREKLRIIGREKDLEPINRRALDIA
                     KTVAAESGAFFAGDICNTNVYDPADAASHKAVRAMFEEQVGWAVDAGVDFIIGETFSY
                     AQEALIALDVVKRAGQTAVITLAMHQADVTRENWSVVDACKRLADAGADVVGLNCIRG
                     PATMMPLLREIRAAVKTHIAALPVPYRTSEREPSFQSLRDAHWHYDEGSRPFPIALDP
                     FTCNRFEIAEFGQQALGLGIRYLGVCCGAGPHHIRALAEAVGKRPPGSRYSADMSKHA
                     FLGSHERIKKVQKDYASKL"
     gene            complement(3585..4634)
                     /locus_tag="VHT22_04285"
     CDS             complement(3585..4634)
                     /locus_tag="VHT22_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEX3136805.1"
                     /translation="MPSPTKAPPISADIRPPRAAPSRGGTRLYTVGFYLVPDFPMMAF
                     AAAIEPLRAANRLSGEKVFAWRLFSRDGNPVRASNGIDVPVGGSIRGDAALDLLLVCA
                     GTANVGAQDKPLAQWLRGLVRGGASVGGISLGAYALAHAGLLDGRRCAVHWENLAAFG
                     DQFPRVRTTTDIFVIDGSRYTCSGGTAGLDMMLQLITDRNGRALANDVSEQFIHPRIR
                     GTQDRQRMAIQARLGVANQKLIAAIGLMESTADEPRDVRQIAADVGLSPRQLERLFAK
                     YLHASPSHHYLELRLDRARELLLQTTKPILDVAVACGFASASHFSRCYRAVYGHKPSD
                     ERSASLVRCDRKPNR"
     gene            complement(4759..5265)
                     /locus_tag="VHT22_04290"
     CDS             complement(4759..5265)
                     /locus_tag="VHT22_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136806.1"
                     /translation="MFKLLFGLTIAVILAGGAAAQPMNSAEQQERAVGPYLYLTDSSG
                     NKVLLSTQFWRNDLKYDDRAFHRFLDVMAALEKRGFRKNDKAVIDWDKPEPVVRCYIY
                     LEDMQTARKAKGTVVSDSRLWCADNGASEHEVRPSDTPKHVDEVMKYFDEYFARAKSK
                     LHQKSATR"
     gene            complement(5337..5747)
                     /locus_tag="VHT22_04295"
     CDS             complement(5337..5747)
                     /locus_tag="VHT22_04295"
                     /inference="COORDINATES: protein motif:HMM:NF019696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HEX3136807.1"
                     /translation="MATTCAIGLAACNTMQGAGKDIERGGEKISDAAARVRADWRVAR
                     DRHEHDYDAARTSCSSGPAAQREACREQARVAYNTRMNEARVTYKRHEMHSVSAEERA
                     EDAYEAARDKCNDFRNEDEDRCIASARSTYAYRK"
     gene            complement(5991..6164)
                     /locus_tag="VHT22_04300"
     CDS             complement(5991..6164)
                     /locus_tag="VHT22_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEX3136808.1"
                     /translation="MLKWALIFFLISLVAGFFGFTGAAESFRGIAKILFFIALAIFLI
                     VLIFGVFLGTLVF"
     gene            complement(6247..6561)
                     /locus_tag="VHT22_04305"
     CDS             complement(6247..6561)
                     /locus_tag="VHT22_04305"
                     /inference="COORDINATES: protein motif:HMM:NF039524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HEX3136809.1"
                     /translation="MSATGLGRNRTPKARDVRDQLTQVESMLGQAAKETGEKASNLFS
                     QVEAKLRSATDAVQELQEDAISRARAAGRVADDYVSDNPWQMIGVAAAVGFLAGALLS
                     RR"
     gene            complement(6607..>7563)
                     /locus_tag="VHT22_04310"
     CDS             complement(6607..>7563)
                     /locus_tag="VHT22_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136810.1"
                     /translation="LVRIPGYPVYYDPQVNSNYFFYDGLYWVYWGDNWYASEWYNGPW
                     QLVAPVYVPQFLLLVPVRYYRAPPVYFRTWYRDGPPRWGEHWGRTWEQRRVGWDRWDR
                     RSVPAAAPLPVYQRQYSGNNYPRAVEQQQVIRSQNYRYQPREAVAQQHFQQHAAQGRL
                     QGQQGQQQVQSQGNGPRARTQTQQPVQHGSAPQAEQQQRQQMNRQRQQQQEQLHQQQQ
                     QQSMQGQGRPPQSQAQMQQHQNAPQERIRPPGPPQAQGPEPRSPPARAAQAGPQGGGG
                     ENHPQARGGENKGAQREAKGEPQGKGEDKGAHKGQVKQDENK"
BASE COUNT         1315 a         2446 c         2439 g         1363 t
ORIGIN      
        1 agaaaggcga tcggggcggt gcccacgttg acagccatcg cggtgcgtat gccggcgaag
       61 atcacgaaca gcgcgttcgg caactcgacc cggcggagga tcgcgccggg gctcatgccc
      121 atcccctgcg cggcgtcgac cagatgcccc ggcacggcgc gcagtcccgc ataggtgttg
      181 cgcacgatcg gaagcagcgt ggcgacccac agaccgacga tcgcgggcaa tgggccaatg
      241 ccgaggaaag tcatcatcag cgcgagcttg cccaaggtcg ggatcgaggt cgccatgttg
      301 accgcctgca tcgtcgcttc cgcatagcgc gccatcgacg gcctggacag ccagatgccg
      361 aggctgacgc cggtgacgat ggccgcgccg ccggataccg cgaccagcac cagatgctgc
      421 ttggtcagat agatgacgtc cttgcggtag cgcaggatgt cggtccacaa gccgcttgcc
      481 accagcaccc agacaatcgc cagcgcgatg ccggccagca ccgcgtaagg cagcatcgag
      541 cgctgtcccg tttgccaact gcgggcggcg gtagtcattg gggcgacgct cccagcgcgc
      601 cggcgggtgc cgcgagtctc gcggcgaccg cgtcgcgtgt gacgatcccg acgaagtggc
      661 cgtcggcgtc gacgcagcgc aggacgtcga cgccctgctc gagaaagagc gtcgccaccc
      721 gccgcagatc gtcggttggg ctgaccgcag gcgcgtgcgg tgcggtcgtg tgatccccgc
      781 cgccggcgcc gttaacgact tcactgacgg agatgcggcg cagtcgcttc aacgttcgat
      841 cgctgccgac gaagctggcg acgaactcgt tggcgggtcg gccgagcaac tcgtctggcg
      901 ccgcatactg ctcgaggcgg ccggcgcgaa agatcgcgat gcggtcggcc atcttcactg
      961 cttcgtcgat atcgtggctg acgaacaata ccgtcttgcg cagcgctttt tgcatgcgca
     1021 gaaactcgtc ctggatgact tcgcgattga ccgggtccac tgcgccgaac ggctcgtcca
     1081 tcaaaagcac gggcggatcg gcggcgagcg cgcgggcgac gccgacacgc tgtgcctggc
     1141 cgccggaaag ctcgttcgga tatcggtcga gaaattcggc gggatccagc gccagcatcg
     1201 ccaagagctc ttcggcgcgc cggcgcatcc gggcgcgatc ccagttcaac agtcgcggca
     1261 caaccccgat gttctcggcc accgtcatgt tgggaaagag cccgatctgc tggatcacat
     1321 acccgatcgt gcgacgcaat tccaccgggt cggcggcgct ggtgtcgcgt ccctcgatga
     1381 aaaccttgcc cgaagtcggc gcaaccagcc gattgatcat gcgcaaggtc gtcgtcttgc
     1441 cgcaaccgga cggccccagc aaaacgcagg tctcgccggc gcccacttcg aaactgacgc
     1501 gatctaccga ggcggcgcca ccgtgcgccg acggatagat tttggtgagc gattcgagcc
     1561 ggatcatgtt tgtggtgcgg tcggacgcat caaccggctc gttccgcggc gcccttcgga
     1621 gtgaggcggc gctgcagcca gagcagcccg tagtcggccg cgatggcgag cagggccagc
     1681 gccagcgcgc cggtaatcaa ttggcggatg tcggtctgcg agattccgcg ggagatgaag
     1741 gtgccgagcc cgccggcgcc gatgtacgcg gcgatcgcgg tcacgccgat gttgatgacg
     1801 acggcgatcc gcacgcccgc gatgatgacc ggcagcgcga tcgggatttc gacacgcaga
     1861 aggcgctgcg aagtcgacat gcccatgccg atcgcggccg agcgcagcgc gggatcgacg
     1921 ttgcgtatcg cggtataggt gttgcgcacg atcggcagct gcgcgtagag catcgcggcg
     1981 atcaccgccg gcaggtaacc gatgccctgg ccgatcttcg acagcagcgg aatcaggacg
     2041 ccgaacaatg cgatcgaagg aatcgtcatc acgatcgagc ccaggtaaag cacggtgtcg
     2101 gcggtgcgtt gactctgggt gattgcaatg cccagcggaa ccccggtcag gattgcaaac
     2161 ccgacggcta cgctgacgat cgagacgtgc tcgaccgtgc gctcgccgat ggctggcagg
     2221 ttgtgcagaa taaaagccaa tgtctccaag acatcctcga aagcggcttg ccgcgccgtg
     2281 ctgcggggcg ctcgccgcca gcatagttgg cgcgccggga ggcaagtgtt acagatacga
     2341 cccgggttgg tccattgcgg actttggcgg cgcggctgga aagacgcgga ccgcacgagg
     2401 tcccggcagc cggacgccgg ccggatgctg cccgggcgtc gcactggcga caatcggcgt
     2461 cgcctggcgg ctggtcggcg accgaccgag gtgcgatagt tgcgccttca cgcccgggac
     2521 atcgccatga ctccaaccgc aacgcaacgg ccttcgaact cgctcacgga acgcctcgat
     2581 caggggccgg tgatctgcgc cgaggggtac gtattcgagc tcgagcggcg cgggtatctt
     2641 caggccggcg ctttcgttcc ggaggtggtc ctagagcatc ccgaagtcgt cgcgcaactg
     2701 caccgcgatt tcgtgcatgc cggatccgat gtcgtggaag cgcttaccta ctacgcgcat
     2761 cgcgaaaagc tgcgcatcat cggccgcgaa aaggacctcg agccgatcaa ccgacgcgca
     2821 ctggacatcg caaagaccgt agcggctgaa agcggcgcct tcttcgccgg agacatctgc
     2881 aacaccaacg tctacgatcc ggccgacgcc gcgtcgcata aggcggtgcg cgcgatgttc
     2941 gaagagcagg tcggctgggc agtcgacgcg ggcgtcgatt tcatcatcgg cgaaacgttt
     3001 tcctatgcgc aggaagcgct gatcgcgctc gacgtggtca agcgcgccgg gcagacagcg
     3061 gtgatcacgc tggcgatgca tcaggccgac gtgacgcgcg agaactggag cgtcgtcgac
     3121 gcgtgcaagc ggctggcgga cgccggcgcc gacgtcgtcg gcctgaattg catccgcggc
     3181 ccggcgacga tgatgccgct gctgcgcgag atacgcgccg cggtcaagac gcatattgcg
     3241 gcgctgccgg ttccctaccg aaccagcgag cgcgagccct cgttccagtc gctgcgcgat
     3301 gcgcactggc attacgacga gggatcccgg ccgttcccga tcgctctaga cccgtttacg
     3361 tgcaaccgct tcgagatcgc cgaattcggc cagcaggcgc tcggcctcgg catccgctat
     3421 ctcggcgtgt gctgcggtgc cgggcctcac cacattcgcg cgctggccga ggccgtgggc
     3481 aagcgcccgc cgggcagcag atactccgcc gacatgtcga agcacgcgtt cctggggtcg
     3541 cacgagcgga tcaagaaggt ccagaaggac tacgcgagca agctctagcg attcggcttg
     3601 cggtcgcaac ggaccagtga cgcggaacgc tcgtcgctcg gcttgtgacc gtagaccgcg
     3661 cgatagcaac ggctgaaatg cgaggccgag gcgaatccgc aggctaccgc cacgtcgaga
     3721 atgggcttgg tggtttgcag cagcaattcc cgcgcccggt cgagccgcaa ctccagatag
     3781 tggtggctcg ggcttgcatg caggtacttc gcgaacaggc gctcgagctg tcttggtgac
     3841 aggccgacgt cggcggcgat ttgccgaaca tcgcgcggtt cgtcggccgt gctttccatg
     3901 agcccgatcg cggcgatcag cttctggttg gccacgccca gccgggcctg gatcgccatg
     3961 cgctgccgat cctgcgttcc gcggatccgt ggatggatga attgctcgga tacgtcgttg
     4021 gccagcgccc gcccgttgcg gtcggtgatc agctgcagca tcatgtccag cccggcagtt
     4081 ccgcccgagc acgtatagcg gctgccatcg atgacgaaga tatcggtcgt ggtgcggaca
     4141 cgcggaaact ggtcgccgaa cgcagccaga ttttcccaat gcactgcgca tcgccggccg
     4201 tcgagcagtc cggcgtgcgc cagcgcgtag gcaccgagac tgatgccgcc gaccgacgcg
     4261 ccgccgcgca ccaagccgcg cagccattgg gcgagcggct tgtcctgcgc gccgacgttg
     4321 gccgtgccgg cgcagaccag caagagatca agggcggcat cgccgcggat cgagccgccg
     4381 accgggacat cgatgccgtt gctcgcacgc accggattgc cgtcgcgtga aaacagccgc
     4441 caggcgaaca ccttctcgcc ggacagacga ttggccgcgc gcagcggctc gattgcggcg
     4501 gcgaaagcca tcatcggaaa atcgggaacc agatagaaac cgacggtgta caagcgtgtg
     4561 ccgccgcgcg acggcgcggc gcgcggcggc cggatatcgg cgctgatcgg cggcgctttc
     4621 gtcggtgacg gcatcggtcg atgttaccgc gctgccgccg accggtcgac gccaggtgat
     4681 cacccggccg tcgcacgtcg agtttcttct acgcgcccaa agtgacgttt gcgacgcgtt
     4741 taccgagcag ccgcgttgct accgcgttgc gctcttctgg tgcagcttgg acttggcccg
     4801 ggcgaaatac tcgtcgaaat acttcatcac ctcgtcgacg tgcttcggcg tatcggacgg
     4861 cctgacttcg tgctctgaag cgccgttgtc cgcgcaccat aaccgggaat cgctgacgac
     4921 ggtgcccttc gctttcctcg ccgtctgcat gtcctcgagg tagatgtagc agcgaacgac
     4981 cggctcgggc ttgtcccagt cgatcacggc cttgtcgttc ttgcggaatc cgcgcttctc
     5041 cagagcggcc atcacatcga ggaagcggtg aaatgcgcgg tcgtcgtatt tcaaatcgtt
     5101 gcgccagaat tgcgtgctga gcagaacctt gttgccgctc gagtcagtca ggtacagata
     5161 cggaccgacc gctctttcct gctgctcggc tgaattcatc ggttgcgccg cggcgccccc
     5221 ggccagaatg acagcaatgg tgagcccgaa cagtaatttg aacatgagag gctcctcttt
     5281 ggtcgcgccg gccgccgacg tcgcccaagc cggccaccgg tgccgcccgt tttcgcttac
     5341 ttgcggtatg cgtacgtcga ccgcgcgctg gctatgcaac gatcttcgtc ctcattgcgg
     5401 aagtcgttgc acttgtcgcg cgccgcctcg taggcgtctt ccgcccgttc ctctgccgat
     5461 acgctatgca tttcgtggcg cttataggta acgcgcgcct cgttcatccg ggtgttatac
     5521 gctacccgcg cctgctcgcg acaggcctcg cgctgcgcgg cggggccgct ggagcagctg
     5581 gtgcgcgctg cgtcgtagtc gtgctcatgt cggtcgcggg cgacgcgcca atcggcgcgc
     5641 actcgcgcag cggcgtccga tattttctct ccgccgcgct cgatgtcctt cccggcgccc
     5701 tgcatcgtat tgcaggcggc aagcccgatc gcgcaggtgg ttgccaagac aagctgtgtc
     5761 catgcattca ttttgaggtt gctcctgagt gttcgtaaca aggaccagtg catcggcccc
     5821 caattgcgtg tccccggcag cctcccgaaa caccggccac ggactcgtga aacccgaaat
     5881 taaccgagcc cccggttcca aaaaatagga cggcctctga atcggatgta ggaatcgggg
     5941 tcaggcagcg cctgccccga agcgcaagcg gcaagcgcgc gttgggtgaa tcagaacact
     6001 agcgtaccca ggaatacgcc gaagatcaga acgatgagaa agatggcaag cgcgataaag
     6061 aacaggatct tggcgatgcc acggaaactt tctgcggcgc cggtaaagcc gaagaaaccg
     6121 gcgaccagag aaatcagaaa aaagatcagc gcccatttca gcatgacgaa ttccttcgag
     6181 cgcgctgact agccgcccag tgcggcccga ctaagcagcg ccgggaagag tttcatcgac
     6241 gaagggtcag cgacgactca gcagcgcccc cgccaggaaa cccacggcag cggcgacacc
     6301 gatcatctgc cagggattgt cgctgacgta atcatcggca acgcgtcccg cggctcgggc
     6361 gcggctgatc gcatcctcct gcagctcctg caccgcgtcg gtggcggaac gaagcttggc
     6421 ttccacttgc gagaacaagt tgctcgcctt ctcccccgtt tccttggcgg cttgtccgag
     6481 catcgattcg acttgcgtca gctggtcgcg gacatcccgc gccttcggcg tgcgattgcg
     6541 acccaatccg gtagcgctca tttttgtctc ctcagtcgat atccgcgtgt cgactacgca
     6601 tcactgctac ttgttttcat cttgcttgac ctgccccttg tgcgctcctt tgtcttcccc
     6661 cttgccctgc ggctcaccct tggcctcgcg ctgggcaccc ttattctcgc cgcctcgcgc
     6721 ttgcgggtga ttctctccgc cgccgccttg cggtccagct tgcgctgccc tggccggcgg
     6781 gctcctgggc tcggggcctt gagcctgcgg cgggcctggg gggcgtatcc tttcctgcgg
     6841 agcattttga tgctgctgca tttgcgcctg cgactgcgga ggacgcccct gcccttgcat
     6901 cgattgctgc tgttgttgct ggtgtagctg ttcttgttgc tgctgccgct gtcgattcat
     6961 ctgttggcgc tgctgttgtt ccgcttgcgg cgccgatcca tgttgcacag gctgctgcgt
     7021 ctgcgtgcgg gcccgcgggc cattgccctg cgactggact tgttgttggc cctgttgtcc
     7081 ctggagccgg ccttgggcag cgtgttgctg aaaatgttgt tgtgctacgg cctcgcgcgg
     7141 ctggtagcgg taattctggg atcgaatcac ctgctgttgc tcgacggcgc gaggatagtt
     7201 gttgcccgaa tattgccgct ggtaaaccgg caacggcgcg gccgcgggga cggaccgtct
     7261 atcccaccgg tcccatccga cccgacgctg ctcccaagta cggccccagt gttcacccca
     7321 gcgcggcggc ccgtcgcgat accaggtccg aaaataaacc ggcggcgccc ggtagtagcg
     7381 caccggaacg agcaagagga actgaggtac gtagaccggc gcgaccagtt gccacggccc
     7441 gttgtaccac tcgcttgcgt accagttgtc tccccagtag acccagtaga gtccgtcgta
     7501 aaaaaagtag ttcgaattta cctgagggtc gtaatagacc gggtagcccg gtatccgaac
     7561 cag
//
LOCUS       DATANS010000302         2797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_57708, whole genome shotgun sequence.
ACCESSION   DATANS010000302 DATANS010000000
VERSION     DATANS010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57708"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VHT22_04315"
     CDS             <1..224
                     /locus_tag="VHT22_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136811.1"
                     /translation="KGEKMQTRHGAKAIAPRVWRDLAALAAEAVAIGVLFSILLALAV
                     LVMTRARPDDSFAGAGEYIAPALIAAASR"
     gene            complement(350..547)
                     /locus_tag="VHT22_04320"
     CDS             complement(350..547)
                     /locus_tag="VHT22_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136812.1"
                     /translation="MSYLGSAIAILVCGGLGTAIAWLVVSPLGLDGVAAGLTTAFAAM
                     IAATLLFACGVALGRALQLFK"
     gene            748..1938
                     /locus_tag="VHT22_04325"
     CDS             748..1938
                     /locus_tag="VHT22_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX3136813.1"
                     /translation="MTADPRNVDLQARDTVRSLVASQIREVANVGMGDADILPFWFGE
                     PDQVTPAYIRDAAVASLHAGETFYTQNFGIPELREAIAAYVSRWHGSVALANVAVTAS
                     GMSALMLSVEALIDPGDRVVCVTPLWPNLTEIPKILGAEVVRVALSFGATGWTLDVAR
                     LLDALTPGTRAVLINSPNNPTGWTIDRAGQAAILEHCRKLGIWIVADDVYERLYYDAP
                     AARACAPSFLDLSSPGDRIVSTNSFSKAWLMTGWRLGWIVAPGTLLPDLGKLIEYNTS
                     CSPTFVQRAAVVAINDGEATIAHTLERYRTARDFLVTELNAIPGVSAAAPTGAMYVFF
                     RVAGVTDSLEFCKRLVREVRLGLAPGSAFGPEGEGFVRWCFAADNDRLADGVARLRRA
                     LGKI"
     gene            2026..>2797
                     /locus_tag="VHT22_04330"
     CDS             2026..>2797
                     /locus_tag="VHT22_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3136814.1"
                     /translation="MIAIACALPMALASAADMSKTLHVSQSVAESGFDPQAISDSYSF
                     DICRAIFESLYTYDYFARPVRMVPNTADGMPQISDGGRTYTVKVKPGIYFASDPAFKG
                     KRRELTAEDYVYTIKRIFDPKIRSYWLYVFDHRLLGLDEALALARKTGRFDYDARIEG
                     LQTVDRYTLRVRFKDPNYTFAYWLADTALSAVAREVVAAHADASNRVMEHPVGTGPYR
                     LKEWVRGQKIVLEANPDFREQTYPAPGEGSEPGDTAIAK"
BASE COUNT          460 a          953 c          915 g          469 t
ORIGIN      
        1 gcaaaggaga gaagatgcag acaagacatg gcgcgaaagc gatcgcaccc cgcgtatggc
       61 gcgatctggc ggcgctggcc gccgaggccg tcgccatcgg cgtgttgttc agcatattgc
      121 tcgcattggc ggtcctggtg atgacgcgcg cgcggcccga cgatagtttt gccggcgccg
      181 gcgagtacat cgcgccggcg ctgatcgctg ccgcatcgcg ctaaacggat tgctgaagga
      241 aatcgctcgc cgagcgggga ggacctttcg gtcggcgcgg ccgcaccgga cgccggaatg
      301 cggcgcggcg ggcgcgaaac cggccgagtc ttccccggtc ggcgacgggc tacttgaaaa
      361 gctgtagcgc ccggcccagc gcgaccccgc acgcgaacaa cagcgttgcg gcgatcatcg
      421 cggcgaacgc tgtcgtcagg ccggcggcga cgccgtcgag gcccagcggc gacacgacca
      481 gccacgcgat cgcggttccc aagcccccgc agacgagtat ggcgatggcg ctgccgaggt
      541 agctcatgcg attccaagcg atggcgacgg cggcgagtat accagcgcgc tcgcgctcgc
      601 ctcgcttgcc gtcacctgac gctcaccacg cacggccagt catttgccac actcattggc
      661 cgctcatttg cgctcatttg ccatccgcgc gggtgcaaag tataaaggcg ctgcgcagac
      721 gcctcgtttg cggcacgcac gcttgcaatg accgccgatc cccgcaacgt cgaccttcaa
      781 gcccgcgata ccgttcgctc gctcgtcgcg tcgcagatcc gtgaagtcgc caacgtcggc
      841 atgggagacg ccgacatcct gccgttctgg ttcggagaac ccgaccaggt gacgccggcg
      901 tacattcgcg atgcagcagt cgcctcgctg cacgccggcg agacgttcta cacgcagaac
      961 tttgggattc ccgagctgcg cgaagccatc gccgcgtacg tgtcgcgctg gcacggcagc
     1021 gtcgccctcg ccaacgtcgc cgtgacggcc tcgggcatgt cggcgctgat gctgtcggtc
     1081 gaggcgctga tcgatccggg cgaccgcgtt gtctgcgtta cgccgctgtg gccgaacctg
     1141 accgagatcc cgaagatcct cggcgccgaa gtcgtcagag tcgcgctgtc gttcggcgcc
     1201 accggctgga cgctcgacgt agcccggctg ctcgacgcgc tgacgccggg cacacgcgcg
     1261 gtgctgatca attcgccgaa caatccgacc ggctggacga tcgatcgcgc cgggcaggcg
     1321 gcgatactcg agcattgccg caagctcggc atctggatcg tcgccgacga cgtctacgag
     1381 cggttgtatt acgacgcgcc ggccgcgcgg gcctgcgccc cttcgttcct cgacttgagc
     1441 agccccggcg accgcatcgt cagcaccaac agcttctcca aggcctggct tatgaccggt
     1501 tggcggctcg gctggatcgt agcgccgggc acgctgttgc ccgacctggg caagctgatc
     1561 gaatacaaca cctcgtgctc gccgacgttc gtgcagcgcg ccgccgtcgt cgcgatcaac
     1621 gacggcgaag cgacgatcgc gcatacgctg gagcgctacc ggaccgcgcg cgatttcctg
     1681 gttaccgagc tgaacgcgat tcccggcgtc agtgccgcgg cgccaaccgg ggcgatgtac
     1741 gtcttttttc gcgtcgccgg cgtcaccgac agcctcgaat tctgcaaacg gctggtgcgc
     1801 gaggtccgtc tggggctggc accgggcagc gccttcggtc ccgagggcga gggcttcgtg
     1861 cgctggtgtt tcgcggcgga caacgatcgg ctcgccgacg gcgtcgcgcg gttgcgccgc
     1921 gcactcggca agatctgacg cgcgatgtcg gcgttcaata cgtccagcca tcgctctccg
     1981 cgtccgccaa ttgcggcgtc agcacggcat gccgcgccgg ccttcatgat cgcgattgcc
     2041 tgcgcgctgc cgatggcgct ggcatctgcg gcggacatgt ccaagaccct gcacgtctcc
     2101 cagtcggtcg ccgagtcggg cttcgatccg caggcgatca gcgattccta ttcgttcgac
     2161 atctgccggg cgatcttcga gtcgctgtac acctacgatt atttcgcacg cccggtgcgc
     2221 atggtcccga acaccgccga cggcatgccg cagatcagcg acggaggccg gacctatacc
     2281 gtcaaggtga agcccggcat ctatttcgcc tccgaccccg cgttcaaggg caagcggcgc
     2341 gagctgaccg ccgaggacta cgtctacacg atcaagcgga ttttcgatcc gaagatacgc
     2401 tcgtactggc tgtatgtttt cgatcatcgc ctgctcgggc tcgacgaggc gcttgcgctc
     2461 gcgcgcaaga ccggccgctt cgattacgac gccaggatag agggcctgca gacggtcgac
     2521 cgctacacgc tgcgggtgcg cttcaaggat cccaactaca cgttcgcgta ttggctcgcc
     2581 gacaccgcgc tgtccgctgt cgcgcgcgaa gtcgtcgcgg cgcacgccga tgcgtcgaac
     2641 cgtgtgatgg agcacccggt cggcaccggt ccgtaccggc tgaaggagtg ggtgcgcggg
     2701 cagaagatcg tgctcgaagc aaaccccgat tttcgggagc agacctaccc ggcgccgggc
     2761 gaaggcagcg agccgggcga cacggcgatc gccaagg
//
LOCUS       DATANS010000303         1794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_184741, whole genome shotgun sequence.
ACCESSION   DATANS010000303 DATANS010000000
VERSION     DATANS010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184741"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VHT22_04335"
     CDS             <1..484
                     /locus_tag="VHT22_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX3136815.1"
                     /translation="LRMRVDRIDALGDGGIAIIDYKTGVLKQPKPQKWFDDRPRASQL
                     GTYTLAQRAAEPGLAVRAVVHAELRTAGVAASGLAADRAAWPALTNIDAAGPPFGNWG
                     ALESWWEAHLGALASELAAGVATVTPRPKPSPCRICGLQALCRIDSVRFEEPEDNGDA
                     "
     gene            471..>1794
                     /locus_tag="VHT22_04340"
     CDS             471..>1794
                     /locus_tag="VHT22_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX3136816.1"
                     /translation="MATPESNGPSDRAAREAALDVARSFIVQAPAGSGKTELLIQRFL
                     ALLARIERPEAIVAMTFTRKATDEIRERIIDALREAASGAPPEQPHRALTWRLSRAVL
                     ERDAALGWELIAHPSRLQVHTIDALCASWMRQAPMAVKLGAMPRLIERADALHLRAAR
                     AELDAGKEDSAAWQRLLDYLDNDGDRLALLIAEMLGRRDQWLRHVIVGDAVALRTRLE
                     QALGAEIAHRLDAVAALLPAGETRRMLDLARYAAGHLAQSNPAHPFAAWARRGALPDT
                     SAESLDDWRSIADWLLTKDGDFYAGINIAQGFPPKGSAREEGAARRDAHKQAMIALLR
                     SLAAVPGLAAALHGIRRLPPPRYDETAWGFIEALLEVLPRAAAQLKLVFAEARAIDFI
                     EASQIALRALGEPDAPSDLLLRLDMRIEHLLVDEFQDKSLSQYELIERL"
BASE COUNT          279 a          622 c          619 g          274 t
ORIGIN      
        1 tcttcgcatg cgcgtcgacc ggatcgatgc cctcggcgat ggcggcatcg cgatcatcga
       61 ctacaagacc ggcgtgctca agcaacccaa accgcaaaaa tggttcgacg atcgaccgcg
      121 cgcatcgcaa ctcggcacct atacgctggc gcaacgcgca gccgagcctg ggctcgcggt
      181 gcgcgcggtg gtccatgccg agcttcgcac cgcgggcgtc gccgcgtcgg gcctcgccgc
      241 tgatcgcgcg gcgtggcctg cgctgacgaa tatcgacgcc gcggggccgc cctttggcaa
      301 ctggggcgcg ctcgagtcct ggtgggaagc gcatctgggc gcgctcgcca gcgagctcgc
      361 ggccggcgtc gcgaccgtaa cgccgcgacc aaagccgtcg ccgtgccgca tctgcggctt
      421 gcaggcgctc tgccgcatcg attcggttcg cttcgaagag ccagaagaca atggcgacgc
      481 ctgaatccaa cggtccttcc gatcgcgccg cgcgcgaggc cgccctcgat gtcgcgcgct
      541 cgttcatcgt ccaggctccg gccgggtcgg gcaagactga gctcctgatc cagcgcttcc
      601 tcgcgctgct cgcgcgcatc gagcggcccg aggcgatcgt cgcgatgacg ttcacgcgca
      661 aggccaccga cgagattcgt gagcgcatca tcgatgcgct gcgggaagcc gccagcggcg
      721 cgccgccgga acagccgcat cgcgcgctta cctggcgcct ctctcgtgcc gtgctcgagc
      781 gcgatgcggc acttggatgg gaactgatcg cgcatccctc gcgcctgcag gtccacacca
      841 tcgacgctct gtgcgcgtca tggatgcggc aggcgccgat ggcggtgaag ctgggcgcaa
      901 tgcccaggct gatcgagcgc gcagacgcgc tgcatttgcg cgcggcgcgg gcagagctcg
      961 atgccggcaa ggaagacagc gcagcctggc agcgcctgct ggattatctg gataacgacg
     1021 gcgatcggct ggccttgctc atcgcagaga tgctcggccg gcgcgatcaa tggctgcgcc
     1081 acgtaatcgt cggcgatgcg gttgcgctcc gcacgcgact cgagcaggca ctgggcgcag
     1141 agatcgcgca ccgactcgac gccgtcgcgg ccctgctgcc ggcgggcgaa acgcggcgga
     1201 tgctcgatct cgcgcgctac gctgcggggc atctcgcgca atccaatcca gcgcatccat
     1261 tcgccgcctg ggcgaggcgc ggcgcgctgc cggatacgag cgcggaatcg ctcgacgact
     1321 ggcgctcgat cgccgactgg ctgctcacga aggacgggga tttctacgcc ggcatcaaca
     1381 ttgcgcaggg cttcccaccc aagggcagcg cgcgcgagga aggcgcggcg cggcgagacg
     1441 cgcacaagca ggcgatgatc gcattgctgc gcagtctggc ggcggtgccg gggctggcgg
     1501 cagcgctgca cggcattcgc cgactgccgc cgccgcgcta cgacgagacg gcctggggct
     1561 tcatcgaggc gctgctcgag gtgctcccgc gcgccgcggc gcagttgaag ctcgtcttcg
     1621 ccgaggcgcg cgcgatcgac ttcatcgagg ccagccagat cgcgctgcgc gccctgggtg
     1681 agcccgacgc gccgtccgac ctgctgctgc ggttggacat gcgcatcgaa cacttgctgg
     1741 tcgacgaatt tcaggacaag tcgctctcgc aatacgagct catcgagcgt ctgg
//
LOCUS       DATANS010000304         3841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_99887, whole genome shotgun sequence.
ACCESSION   DATANS010000304 DATANS010000000
VERSION     DATANS010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99887"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VHT22_04345"
     CDS             <1..366
                     /locus_tag="VHT22_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX3136817.1"
                     /translation="RLASAGFGLACVTNKTSRFVRPHLAQAGLGAYFSLVVGGDSLEA
                     KKPDPLPLQHAARHFCVAPDRLLMLGDSANDTQAARAAGCPVFCVPYGYNEGRPVQSL
                     DCDGIVSSLDELPERLRLG"
     gene            528..2006
                     /gene="trpE"
                     /locus_tag="VHT22_04350"
     CDS             528..2006
                     /gene="trpE"
                     /locus_tag="VHT22_04350"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX3136818.1"
                     /translation="MTEAEFRALAAQGYNRIPLVLESFADLDTPLSLYIKLANQRFTY
                     LLESVVGGERFGRYSFIGLPAKVRIRSRGRSIEVEDRGGIVERHEGDPLAFVGEFLRR
                     YRAAPRPGLPRFCGGVAGYFGYDTVRHIEHRLADSGPAASAALADVPDILLLLTEELA
                     VVDNLSGKISLIVYADPAEPNAFWEARDRLQALRRKLRDPVSIPYQTATEVSVAESEF
                     GADGYQAAVARSREYIAAGDVMQVVISQRMRRPFAAPPLSLYRALRSLNPSPYMFYYD
                     FGDFHVVGASPEILVRKEGTGITLRPIAGTRPRGATREADEVLATELLADPKEIAEHV
                     MLLDLGRNDVGRVAQTGTVQVTDRMVIERYSHVMHIVSNVEGILKPGLTSIDVLRAAF
                     PAGTVSGAPKVRAMEIIDELEPTRRGIYSGAVGYISFQDDMDTAIALRTAVIKDGMLY
                     AQAGGGIVHDSSPEGEWQETMNKLRALLRAADMVQLGLDAEG"
     gene            2011..2577
                     /locus_tag="VHT22_04355"
     CDS             2011..2577
                     /locus_tag="VHT22_04355"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX3136819.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGADVHVHRNDAITLDQIAAWQP
                     ERIVISPGPCTPDQAGISVALIERFAGKIPILGVCLGHQAIGQAFGGRIVRAQRVMHG
                     KVSTISHSGGGVFAGLANPLTATRYHSLVIERASMPACLEITATSDDGEIMGVRHRTA
                     PVEGVQFHPEAILTEHGHALLSNFLAAH"
     gene            2600..3640
                     /gene="trpD"
                     /locus_tag="VHT22_04360"
     CDS             2600..3640
                     /gene="trpD"
                     /locus_tag="VHT22_04360"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_035822072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX3136820.1"
                     /translation="MSIDLQQALQRTIEHREIFHDEMLALMRKIMSGEASPVMVAALT
                     VGLRVKKETIGEIAAAAQVMREFSTKVEVADHQRLVDIVGTGGDASHTFNISTASMFV
                     AAAAGARVAKHGGRSVSSRSGSADVLEALGANIMLKPEQVAACIGETGIGFMFAPNHH
                     SAMKHAAPVRKELGIRTIFNILGPLTNPAGAPNQLLGVFHPDLVGIQVRVLQRLGSKH
                     VLVVYGKDGMDEVSLGAATMVGELKDGEVREYEIHPEDFGLKMISNRGLKVEDAVESK
                     TMLLEALDNIEGTPREIVVLNAGVALYAANAAATIPEGIERARAAVVSGAARRKLDQF
                     VAATQKLGAGAK"
     gene            3637..>3841
                     /locus_tag="VHT22_04365"
     CDS             3637..>3841
                     /locus_tag="VHT22_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007341502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136821.1"
                     /translation="MKPPAHRDRDILTRIITTKAEEVIGAELARPFAEVEAAARATAA
                     PRGFERAIRQRIAAGAPAVIAEIK"
BASE COUNT          664 a         1236 c         1295 g          646 t
ORIGIN      
        1 aggctcgcca gcgcgggctt cgggctcgcc tgcgtgacca acaagaccag ccgtttcgtg
       61 cgtccgcatc tggcccaggc cggattgggc gcgtatttca gcctggtcgt cggcggcgat
      121 tcgctcgagg cgaagaaacc ggatccgctg ccgctgcagc atgccgccag gcatttttgc
      181 gtcgcgccgg accgtctgct gatgctcggc gattcggcca acgatacgca ggcggcgcgt
      241 gcggcgggat gcccggtttt ctgcgtgcct tacgggtaca acgagggaag gcccgtgcaa
      301 agcctcgact gcgatggtat agtttcctcg ctggatgaac tcccggaacg gttacgacta
      361 ggttgaacga tgggcttcga cgttcacaac tgcaaaagcg gccgatggtc atcgccgcgc
      421 ggcaacgatt ggcgctggcg gcgcccatcg tagtccgccc cggcgtcgcg cgctgaccgc
      481 gacgccttcg cagtgtcgtt gcagttgtag aaaaccgttg gagcatcatg accgaagccg
      541 aattccgcgc gctcgcggcg caggggtata accgcattcc gctcgtgctc gagtcgttcg
      601 ccgacctcga cacgccgctt tccctctaca tcaagctcgc caaccagcgt tttacctatt
      661 tgctggaatc ggtcgtcggc ggcgagcgct tcgggcgcta ctctttcatc ggcctgccgg
      721 cgaaagtccg gatccgcagc cgcggacgct cgatcgaggt cgaggatcga ggcggcatcg
      781 tcgagcgcca cgaaggcgat ccgctggcgt tcgtcggcga gttcctgcgt cgttatcgcg
      841 ccgcgccgcg gcccgggcta ccgcgcttct gcggcggcgt cgccggctac ttcggctacg
      901 acacggtccg ccacatcgag caccggctcg ccgattccgg ccccgccgct tctgctgcgc
      961 tggccgacgt gcccgacatc ctgctgctgc tgaccgagga gctagcggtc gtggacaacc
     1021 tctcgggaaa gatttcgctg atcgtttatg cagatccggc cgagccgaat gccttctggg
     1081 aggcgcgcga tcggctgcag gcgctgcggc gcaagctccg cgacccggta tctatccctt
     1141 atcagacggc gaccgaggtc agcgtcgccg agagcgaatt cggggccgac ggctaccagg
     1201 ccgccgtggc ccgctcgcga gagtacattg ccgcgggcga cgtgatgcag gtcgtgatat
     1261 cgcagcggat gcggcggccg tttgcagcac ccccgctttc cctgtaccgc gcgctgcgct
     1321 cgctcaatcc gtcgccgtac atgttctatt acgatttcgg cgactttcac gtcgtcggcg
     1381 cctctcccga gattctggtg cgcaaggagg gcaccgggat cacgcttcgg ccgatcgcgg
     1441 gcacgcggcc gcgcggcgcg acgcgcgagg ccgacgaggt gctcgcaacc gagctgctcg
     1501 ccgaccccaa ggagatcgcc gagcacgtga tgctgctcga tctgggacga aacgacgtcg
     1561 gccgcgttgc gcagacgggg accgtgcagg tcaccgaccg catggtgatc gagcgctatt
     1621 cgcacgtgat gcacatagtg tcgaacgtcg aggggatatt gaagcctgga ctgaccagca
     1681 tcgacgttct gcgcgcggca tttcccgccg gaacggtcag cggcgcgccc aaggtgcgcg
     1741 cgatggaaat catcgatgag ctggagccga cgcgccgcgg catttattcg ggcgcggtcg
     1801 gctacatcag cttccaggac gacatggata cggcgatcgc gctgcgcacg gcggtgatca
     1861 aggacggcat gctctacgcg caggcggggg gcggcatcgt ccacgattcc tcgccggaag
     1921 gcgagtggca ggagacgatg aacaagctgc gggcgctact gcgcgccgcg gatatggtgc
     1981 agctgggcct ggacgcggag ggctagaacg atgctgctga tgatcgacaa ctacgacagc
     2041 tttacctaca acctggtcca gtatttcggc gagctcggcg cggacgtgca cgtgcatcgc
     2101 aacgacgcca tcacactgga ccagatcgcg gcgtggcagc ccgaaagaat cgtcatctcg
     2161 ccaggaccct gcacgccgga ccaggccggt atctcggtgg cgctgatcga gcgcttcgcc
     2221 gggaagatcc cgatcctggg cgtctgtctc ggccaccagg cgatcggaca ggcgttcggc
     2281 ggccggatcg ttcgcgcgca gcgcgtcatg cacgggaaag tctcgacgat ctcgcacagc
     2341 ggcggcggcg tcttcgccgg tctcgccaac ccgctgaccg cgacccgcta tcattcgctg
     2401 gttatcgagc gcgcctcgat gccggcctgc cttgagatca ccgcgaccag cgacgacggc
     2461 gaaatcatgg gcgtgcgcca tcgcacggcg ccggtcgagg gcgtccagtt tcatccggaa
     2521 gcgatattga ccgagcacgg tcacgcgctg ctgtcgaatt ttctggccgc gcactagcga
     2581 cacaaagacg gagctaccga tgtccatcga cttgcagcag gcgttgcagc gcacgataga
     2641 gcaccgcgag atatttcacg acgagatgct tgcgttgatg cgcaagatca tgagcggcga
     2701 ggcctcgccg gtgatggtcg ccgcgctgac ggtgggcctg cgcgtgaaaa aggaaacgat
     2761 cggcgagatc gccgcggcgg cgcaggtgat gcgcgagttc tcgaccaagg tcgaggttgc
     2821 cgaccaccag cggctggtcg acatcgtcgg gaccggcggc gatgcctcgc atacgttcaa
     2881 catttccacc gcgtcgatgt tcgtcgccgc cgcggcgggc gcgcgcgtgg ccaagcacgg
     2941 cgggcgctcg gtgtcgtcgc gatcaggaag cgccgacgtt ctcgaggcgc tcggcgccaa
     3001 cataatgctg aagcccgagc aggtcgccgc ctgcataggc gagaccggaa tcgggttcat
     3061 gttcgcgccc aaccaccaca gcgcgatgaa gcatgcggcg ccggtgcgca aggagttggg
     3121 tatccgcacg atattcaata tcctggggcc gctgaccaat ccggccggcg cgccgaatca
     3181 gctgctcggc gtgttccatc ccgacctggt cggcatccag gtccgcgtgc tgcagcgtct
     3241 cgggagcaag catgttctgg tcgtctacgg caaggacggc atggacgagg tttcgctcgg
     3301 tgccgcgacg atggtcggcg agctcaagga cggcgaagtg cgcgaatacg agatccatcc
     3361 cgaggacttc ggcttgaaga tgatttccaa ccgcggcctc aaggtcgagg atgcggtcga
     3421 atcgaagacg atgctgctgg aggcgctgga caacatcgag gggacgccgc gcgaaatcgt
     3481 cgtgctcaat gcgggtgtcg cgctgtatgc ggcgaatgcc gccgcgacga ttcccgaggg
     3541 gatcgagcgg gcgcgcgccg ctgtcgtctc gggtgccgcc aggcgaaagc tcgatcagtt
     3601 cgtcgccgca acccaaaagc tcggcgcggg cgccaaatga agccgccggc gcatcgcgac
     3661 cgcgacatcc tgaccaggat catcacgacc aaggccgagg aagtcatcgg agccgagctc
     3721 gcgcgaccgt tcgccgaagt cgaggccgct gcgcgcgcga ccgccgcgcc acggggattc
     3781 gaacgggcga tccggcagcg aatcgccgcg ggcgcgccgg cggtcatcgc ggaaatcaag
     3841 c
//
LOCUS       DATANS010000305         2958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_393969, whole genome shotgun sequence.
ACCESSION   DATANS010000305 DATANS010000000
VERSION     DATANS010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393969"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VHT22_04370"
     CDS             complement(<1..543)
                     /locus_tag="VHT22_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3136822.1"
                     /translation="MSARPWLAIAGVVVCSAALAQQAPPPPPSSSITQPAPPPGLSVE
                     LAAAAKLVREGQYDEARSKVDAVLAGDAKNPQARFIRGVIETDEGDGDEAIATFQGLT
                     EDYPELPEPHNNLAVIWAKRGSYDKARIELQAALVINPDYAIAHENLGDVYSRLAVAE
                     FERATTLDKGNKSAQAKLKLV"
     gene            complement(604..1458)
                     /gene="nudC"
                     /locus_tag="VHT22_04375"
     CDS             complement(604..1458)
                     /gene="nudC"
                     /locus_tag="VHT22_04375"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEX3136823.1"
                     /translation="MNTPAGFEPGVVMPAGLAGNAICFVWRDDRILARAAEPPTLPTL
                     ADVVRLGIDGVRHYLGRYRGTDCIAFHVASDSPDIDGWQWRGLRSLFLQLPEAELALA
                     GRSFQISEWDRSHRFCGRCGAAMRDKPGERAKECTACGHIAYPRISPAMMVLVTRGFD
                     LLLARANRFPGAMYSALAGFVEAGESIEDCIHREVLEEVGIEVRDLRYFTSQAWPFPH
                     SLMIAYTAEYAGGDMRPCDAEIADAQWFSIDALPQLPSPISVSRKLIDATIERLRQAR
                     HQEALLRR"
     gene            complement(1472..2608)
                     /locus_tag="VHT22_04380"
     CDS             complement(1472..2608)
                     /locus_tag="VHT22_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3136824.1"
                     /translation="MNRLLKSLFALALVAGFHQARAADPGITPTSILIGQSAAFSGPA
                     MELGTEMRAGALAYFQAINAAGGINGRKIELRSLDDGYEPDRAAANTKKLIDDGVFLL
                     FGYVGTPTSNASKPIFTASRVPFVGPFTGAESLRNPLNRYIFNIRASYFDETDKIVAQ
                     MTGQTLDKIAVFYQNDDYGKAGLAGVQRAMDKRNLKIVATGTVERNTVDVTAAVKSIS
                     KVEPQAIIMISAYKSCAAFIKAMRAAGFNPQFMNVSFVGSKALAHETGLEGRGVGISQ
                     VVPFPWNFSAPVVKEYQQLFEASTKKQEYSFTSLEGFIAAKTLVEGLRRTGGELTRER
                     FINAMETLRDFDLGGYWITFTPTSHNGSKFVELTVIGKDERFLR"
     gene            2788..2958
                     /gene="prfB"
                     /locus_tag="VHT22_04385"
                     /pseudo
     CDS             2788..2958
                     /gene="prfB"
                     /locus_tag="VHT22_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435212.1"
                     /note="recognizes the termination signals UGA and UAA
                     during protein translation a specificity which is
                     dependent on amino acid residues residing in loops of the
                     L-shaped tRNA-like molecule of RF2; in some organisms
                     control of PrfB protein levels is maintained through a +1
                     ribosomal frameshifting mechanism; this protein is similar
                     to release factor 1; frameshifted; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
BASE COUNT          520 a          937 c          975 g          526 t
ORIGIN      
        1 caccagcttg agcttcgctt gcgcgctctt gttgcccttg tcgagcgtgg tcgcccgctc
       61 aaattcggcg acggcgagcc gcgaatagac atcgcccaga ttctcgtgcg cgatcgcata
      121 gtccggattg atcacaagcg cggcctggag ttcaattcgc gccttgtcgt agctgccgcg
      181 cttggcccag attaccgcca gattattgtg cggctcgggc agctcggggt aatcctcggt
      241 caggccctgg aacgtcgcga tcgcctcatc gccgtcgccc tcgtcggttt cgatgacgcc
      301 tcggatgaag cgcgcctgcg gattcttcgc atcgccggca aggacggcat cgaccttgct
      361 gcgcgcctca tcgtattgac cctcgcggac cagcttcgcg gcagcggcga gctcgaccga
      421 caagccgggc ggcggcgcgg gctgggtgat cgacgagctc ggcggcggcg gcggcgcctg
      481 ctgggcgagt gcggcgctgc agacgacgac tccggcgatg gcgagccacg ggcgtgcgga
      541 catggattag gactgcctgg gaccggatgg aacgggcaat tctagcagag ccccgtgtgg
      601 gcttcagcgg cgtaaaagag cctcctggtg gcgcgcctgc cgcagtcgct cgatcgtcgc
      661 atcgatcagc ttgcgcgaaa cgctgatcgg cgagggcagc tgcggcagcg cgtcgatcga
      721 gaaccattgc gcatcggcaa tctcggcgtc gcagggacgc atgtcgccgc ccgcatattc
      781 ggcggtgtac gcgatcatca gcgagtgcgg gaatggccac gcctggctgg tgaaatagcg
      841 gaggtcgcgg acctcgatgc cgacttcctc gagcacctcg cgatggatgc aatcctcgat
      901 cgactcgccg gcctcgacga atccggccag cgcgctgtac atcgccccgg ggaagcgatt
      961 ggcgcgcgcc agcagcaggt cgaagccacg ggtgaccagg accatcatcg ccggcgaaat
     1021 gcgcggatag gcgatgtgac cgcaagccgt gcattccttc gcgcgctcac ccggcttgtc
     1081 gcgcatcgcc gcgccgcagc ggccgcagaa gcggtggctg cggtcccact cggagatctg
     1141 gaacgaccgg cccgccagcg cgagctccgc ctccggcaat tgcaggaaca gcgagcgcaa
     1201 tccgcgccac tgccagccgt cgatgtccgg cgaatcgctg gcgacgtgga aggcaatgca
     1261 gtcggtgccg cggtagcggc ccagatagtg acgcacgccg tcgatgccca gccggacgac
     1321 atcggccagc gtcggcaaag ttggtggctc ggccgcccgc gcaagtatcc gatcgtcgcg
     1381 ccagacaaag caaatcgcgt tgcccgcgag cccggccggc atcacgacgc cgggctcgaa
     1441 cccggcgggt gtattcatca ggggactggc ctcaacggag gaaccgctcg tccttcccga
     1501 tgaccgtcag ctcgacgaat ttcgagccgt tgtggctggt cggcgtgaac gtgatccagt
     1561 agccgccgag gtcgaagtcg cgcagcgttt ccatcgcgtt gatgaatcgc tcgcgcgtca
     1621 gttcgccgcc ggtgcggcga aggccttcga ccagcgtctt ggcggcgatg aatccttcca
     1681 ggctggtgaa cgagtactcc tgcttcttgg tcgacgcctc gaacagctgc tggtactcct
     1741 tgaccaccgg ggccgagaaa ttccacggga agggcaccac ctgcgatatg ccgacgccgc
     1801 gtccttcgag gccggtctcg tgcgccagcg ccttgctacc cacgaacgac acgttcatga
     1861 attgcgggtt gaagcctgcc gcccgcatcg ccttgatgaa cgcggcgcag gacttgtacg
     1921 ccgagatcat gatgatcgcc tgcggttcga ccttggagat cgatttcacg gcggcggtga
     1981 catcgacggt gttgcgctcg acggtaccgg tcgcgacgat cttcaggttg cgcttgtcca
     2041 ttgcgcgctg aactccggca agaccggcct tgccgtaatc gtcgttctgg tagaagacgg
     2101 cgatcttgtc gagcgtctgc ccggtcatct gcgcgacgat cttgtcggtc tcgtcgaaat
     2161 agctggcgcg gatgttgaat atgtagcgat tcagggggtt gcgcagcgat tcggcgcccg
     2221 tgaacgggcc gacgaatgga acgcgcgacg cggtgaaaat cggcttcgat gcattggacg
     2281 tcggcgtgcc gacgtagccg aacagcagga acactccgtc gtcgatcagt ttcttggtat
     2341 tggcggcggc ccggtcgggc tcgtagccat cgtcgagcga gcgcaactcg atcttgcgcc
     2401 cgttgatgcc gccggcggcg ttgattgcct ggaaataggc cagcgcgccg gcgcgcattt
     2461 cggtgccgag ctccatcgcc ggccccgaga acgctgccga ctgcccgatc aggatcgagg
     2521 tcggcgtgat gcccggatcg gcggcccgcg cctgatgaaa acccgcgacc agcgccagcg
     2581 cgaacaggga cttgagcagt ctgttcatct tcctcctcga agggtctcgg tctaaccgca
     2641 gcgaccggcg tttcgccacg agcctcgtcc tatcataggg gattcccgtg ggcatgggaa
     2701 acatccgggc ctgtttgcac gtcggagccg gtgtagcgac gggtataatc gcaacaatcg
     2761 ataccggatc caaagaaaag agcgcccatg gaagcggaac gcctgaatca aatcgctaat
     2821 cagctcgccg atctgcagca tcgcgccacc gatctgcgga ggtatctttg acttcgatgt
     2881 gaaggcatcg cgcttcgagg aagtggcgag ggagctcgaa gacccgcaca tctgggaaac
     2941 gcccgagcgc gcgcagca
//
LOCUS       DATANS010000306         3746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_351517, whole genome shotgun sequence.
ACCESSION   DATANS010000306 DATANS010000000
VERSION     DATANS010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351517"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VHT22_04390"
     CDS             <1..655
                     /locus_tag="VHT22_04390"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEX3136825.1"
                     /translation="CADIGAYSKAEQKALAFILPKLEKQDTILVCTSSEQLGNLAAEG
                     RFDPALLSILSAGAVMLPPLRVRRDDLPALISRFWREITASQRTLGTLQGSAVSALAA
                     AYWPGNLDHLHNVIANLALTIDSEITGEHVRRLLGESALPPSAALAPEVTARFFELPL
                     REAREAFERIYFEQLLGKEQSNMSRVAERAGLERTHLYRKLKQLNIRFSRRSDEGAC"
     gene            649..2556
                     /locus_tag="VHT22_04395"
     CDS             649..2556
                     /locus_tag="VHT22_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEX3136826.1"
                     /translation="MLTGTQAGAATAPADVAKRPTAPLVLGAIGIVFGDIGTSPLYTL
                     RECFTGPHGLPLTADNVLGVLSVIFWALMIVVTLKYVTLIMRADNNGEGGIMALTALV
                     SRGLDDRRARWWLVGLGIFGAAMFYGDGMITPAISVLSAVEGLEVLAPGLKSYVIPVT
                     LVILLALFSIQRHGTAKVGGLFGPLMCVWFATIAVLGAAAVWQDPRVLMALLPSYAFS
                     FLTGNPLAAFLALGAVVLAVTGTEALYADMGHFGVSPIRRAWLYLVAPALVLNYFGQG
                     ALIIAEPEAIKNPFYLLAPAWALLPMVILATAATVIASQAVISGAFSLTRQAIQMGYC
                     PRLTIMHTSERQIGQIYVPFINWMLFFAVMLLVAGFKTSSNLAAAYGIAVTLAMAIDS
                     LLIFVVLTRLWRWNAFAALAIVVPLVAIDVAFLSSNLLKIPEGGWFPIVVGGIVFTLL
                     TTWKRGRAILLERLNAETMPIDLFIESISANPPTRVPGTAVFLTSTPNRAPHALLHNL
                     KHNKVLHERVVLLTMTTRDIPTVPLPDRVQIRDLGCNFQQFEAFFGFKEDPDVPELLE
                     ECGRRGQPFDMMETSFFVSRETLIATETPGMALWRERLFVSMAKNATKAIEFFNVPTN
                     RVVELGTQVEL"
     gene            complement(2617..3465)
                     /gene="rpoH"
                     /locus_tag="VHT22_04400"
     CDS             complement(2617..3465)
                     /gene="rpoH"
                     /locus_tag="VHT22_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEX3136827.1"
                     /translation="MNTLAFPTPVSLGSLDHYIQAVNRFPLLTAEQETELGRKLQRDN
                     DLEAARSLVLSHLRLVVAVSRNYLGYGLPQADLIQEGNIGLMKAVRRFDPERGVRLVS
                     FALHWIRAEIHEHILRNWRLVKIATTKAQRKLFFNLRSMKPKLGPLTRTEANGIAKAL
                     GVKPEEVFEMETRLSGQDIAIDPPAGDDGETMTPSAYLTDSDAEPVQILEREETARNR
                     SVGLKSALSRLDSRSRDIIEARWLREDDPATLQDLADQYGVSAERIRQIESKALKTMR
                     GEMASL"
BASE COUNT          603 a         1198 c         1213 g          732 t
ORIGIN      
        1 ctgcgccgac atcggcgctt attcgaaagc agagcagaag gcgctcgcct tcatattgcc
       61 caagctggaa aagcaggaca cgattcttgt ctgcaccagc agcgagcaac tcggtaacct
      121 cgcagccgag ggtcgcttcg atccggctct gttgtcgatc ctgtccgccg gcgcggtcat
      181 gctgccgcct ctgcgagtgc ggcgcgacga cctgccggcg ctgatatcgc gcttctggcg
      241 cgagatcacc gccagtcagc gtacgctcgg gacgctgcag gggagcgccg tgtcggcgct
      301 ggccgctgcg tactggccgg gcaatctcga ccacctgcac aacgtgatcg ccaacctcgc
      361 tttgacgatc gacagcgaaa tcaccggcga gcacgtgcgg cgtcttctgg gcgaatcggc
      421 gctgccgccg tcggcggcgc ttgcgccgga ggtcaccgcg cgctttttcg agctgccgct
      481 gcgcgaggcg cgcgaagcct tcgaacggat ctacttcgag cagttgcttg gcaaggaaca
      541 gagcaacatg agccgggtcg ccgaacgcgc cggtctggaa cgcacgcatc tgtaccgcaa
      601 gctcaaacag ctcaacatcc gcttttcgcg tcgctccgac gaaggagcat gctgaccggg
      661 acgcaggctg gcgcggcgac agctccggcc gatgtggcga agagaccgac ggccccgctg
      721 gttctcggcg cgatcggtat cgttttcggc gatatcggaa ccagcccgct ctatacgctg
      781 cgcgaatgct tcaccgggcc gcacggcctg ccgctgaccg ccgacaatgt gcttggcgtc
      841 ctgtcggtga tcttctgggc gctgatgatc gtcgtcaccc tcaagtacgt gacgcttatc
      901 atgcgggccg acaataacgg cgagggcggc atcatggcgc tcaccgcgct ggtatcgcgc
      961 ggtctcgacg accgtcgggc acgctggtgg ctggtcggac tggggatttt cggcgccgcg
     1021 atgttctacg gcgacgggat gatcacgccg gcaatctcgg tcttgtccgc ggtcgagggc
     1081 ctcgaagtcc tcgcgccggg tctcaagagc tatgtcatcc cggtgaccct ggtaatcctg
     1141 ctcgcgctgt tctcgatcca gcgccacggg acggcgaagg tcggcggact gttcggcccg
     1201 ctgatgtgcg tctggttcgc gacgatcgcg gtattgggtg cggccgcggt ctggcaggac
     1261 ccgcgggtgc tgatggcgtt gctgccgtcc tacgcattct cgtttctgac cggcaacccg
     1321 ctggccgcgt ttctcgccct cggggcggtc gtgctagccg tcaccggcac cgaagcgctg
     1381 tacgccgaca tgggccattt cggcgtatcg ccgatccggc gtgcctggct gtatctggtg
     1441 gcgcccgccc ttgtgctgaa ttatttcggc cagggcgcac tgatcattgc cgagccggag
     1501 gcgatcaaga atccgttcta tctgctggcg ccggcatggg cgctgctgcc gatggtgatt
     1561 ctcgcgaccg ccgcgacggt gatcgcgtcg caggcagtga tttccggcgc gttctcgctg
     1621 acgcggcagg cgatccagat gggctattgc ccgcggctga cgatcatgca cacgtccgag
     1681 cggcagatcg ggcagatcta cgtgccgttc atcaactgga tgctgttctt tgcggtgatg
     1741 ctgctggtcg ccggcttcaa gacgtccagc aatctggcgg ccgcctacgg catcgcggtg
     1801 acactcgcga tggcgatcga ctcgctgctg atttttgtcg tgctgacccg gctgtggcgc
     1861 tggaacgctt tcgcggccct cgcgatcgtc gtgccgctgg tcgcgataga cgtcgccttc
     1921 ctgtcgtcga acctgctgaa gatcccggaa ggcgggtggt tcccgatcgt cgtcggaggc
     1981 atcgtattca cgttgctgac gacgtggaag cgcggccgcg cgatcctgct cgagcgcctg
     2041 aacgccgaga cgatgccgat cgacctgttc atcgaaagca tctcggccaa cccgccgacg
     2101 cgcgtgccgg gcaccgcagt tttcctcacc agcacgccga atcgcgcgcc gcacgcgctg
     2161 ctgcacaacc tcaagcacaa caaggtcctg cacgagcgcg tcgttcttct gacgatgacg
     2221 acgcgggaca tcccgaccgt cccattgccc gatcgcgtgc aaatccgcga tctgggctgc
     2281 aacttccagc aattcgaggc gtttttcgga ttcaaggaag atccggacgt gccggagctg
     2341 ctggaggaat gcggacgccg cggtcagccg ttcgatatga tggaaacctc gttcttcgtc
     2401 tcgcgcgaaa cgctgatcgc tacggagacg ccgggcatgg ctctctggcg cgagcgcctg
     2461 ttcgtgtcga tggccaagaa tgcgaccaag gcgatcgagt ttttcaacgt cccgaccaat
     2521 cgggtcgtcg agctcggtac gcaggtcgag ctctagcgcg cagacaaaaa cgccggctca
     2581 tcgagagccg gcgtcggaat gcactggtcg tgtagtctag agcgacgcca tttcgccgcg
     2641 catcgtctta agcgccttgc tttcgatctg gcggatgcgc tcggccgaga cgccgtactg
     2701 gtcggccaga tcctgcagcg tcgccgggtc gtcttcgcgc agccagcggg cctcgatgat
     2761 gtcgcggctg cggctgtcca gcctcgagag cgcgctcttc aagccgacgc tgcggttgcg
     2821 cgcagtctct tcgcgctcca gtatctgcac cggctcggcg tcgctgtcgg tcagataggc
     2881 gctaggcgtc atcgtttcgc cgtcgtctcc ggccggcggg tcgatggcga tgtcctgccc
     2941 ggacagcctt gtctccattt cgaacacttc ctcgggcttg acgccgagcg ccttggcgat
     3001 gccgttcgcc tcggtgcggg tcagcgggcc gagcttcggc ttcatgctgc gcaggttgaa
     3061 gaaaagcttc cgctgcgcct tggtggtcgc gatcttgacc agccgccagt tacgcaggat
     3121 gtgctcgtgg atctcggccc ggatccagtg caaggcaaac gacaccaggc gaacgccgcg
     3181 ctccggatcg aagcggcgaa ccgccttcat caagccgata ttgccttcct ggatcaaatc
     3241 ggcctgcggg agcccgtacc cgaggtaatt gcggctgacg gcgacgacca gccgaaggtg
     3301 ggacaggacg agcgagcggg ccgcttccag atcgttgtcg cgctgcagct tgcggccgag
     3361 ctcggtctcc tgctcagcgg tcagcagcgg aaaacggttc accgcctgga tatagtgatc
     3421 cagactcccc agcgaaaccg gggtcgggaa agccagcgta ttcatgggca ttaaaaagct
     3481 ccttggcgac gaggcgatat tttagcactc ggcttgtcag agtgctaagg cgccccgaag
     3541 ttccgccaca tggccaaggc ctgcgttggg gtgagcggtc cgtgcgcaac caccgacccg
     3601 gcggaccgat taccaggcgt cgccttaccg acgggagcac cgggttgcta ccgttggcgt
     3661 gggacggtcg ggggtcgagt tagtgcttac ttatcagcag ccgctttgcg acggccatcg
     3721 ccattccgtt acccgatgag cgcatc
//
LOCUS       DATANS010000307         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_57864, whole genome shotgun sequence.
ACCESSION   DATANS010000307 DATANS010000000
VERSION     DATANS010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57864"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            397..>2083
                     /locus_tag="VHT22_04405"
     CDS             397..>2083
                     /locus_tag="VHT22_04405"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX3136828.1"
                     /translation="MLSPSRSLVRGLFAAALLWFVGAAAAALPAGVTAGASVEGISEY
                     ALANGLKVLLFPDASQPKVTVNITYLVGSRMENYGETGMAHLLEHMVFKGTKTRGNLM
                     TGLAQRGMAFNGTTWYDRTNYFETFPASDESLDWALGMEADRMVNSTVLRKDLDSEMT
                     VVRNEMERNENSAVRILIQRTTAAGFDWHAYGKDTIGARTDVERVDIGRLQAFYRLYY
                     QPDNAVLTIAGAFDPERALALVAKYFGPLPKPSRVLPALYTDEPVQDGAREVTLRRVG
                     NTQWLSAMYHTVPGAHPDAVAISAAVDAMTVSPGGRLYKALVEAKKAAAVDDFVYTGF
                     DPGFAMFLAQVPENESLDTARTTMLQVIEDVAKQPFTQGEIDRVRAKALKSIDETIND
                     PQRLGIALSGSIAAGDWRLFFLQRDRWRALTPADVDRVAGAYFKAANRTVGEFIPDAK
                     PDRAPAPPTVNIAAMVKDYKGDPAVAAGETFDATPANLDARAQRYTLANGMKVALLPK
                     KTRGETVHFYLSMHYGDEASIKGREGEARLAGSMLLRGTSKHSRQEIEDTLDRL"
BASE COUNT          375 a          703 c          673 g          332 t
ORIGIN      
        1 accgtcgaac aggctcgccg cggtgacgaa tcggatcttg tgcgccggct tgtagaccag
       61 cggctttttg gcgatggcga ggtcgttcat cactgctccg gcggtcggat ccgggttccg
      121 gcgggggtgc ctgcgcgcag cgccatggcc ggaaacgcgc ccgcgccggc caggaactgc
      181 gctggaaagc ttactcccgg cgctctgccg tcgacattat atgccgttta cgtaaacgtc
      241 aatatggcgc aaggctcacg gggcggtcgg gaacgccttc gggccgcggc ggtcctacag
      301 cggccgcaaa acgcccggtc gccgcgaggc agttttcatc ggtcctgccg ccgctatact
      361 caatccccgc actccccgat gcagaaggaa ttgcccatgt tatcgccgtc acgatcgctg
      421 gtccgcggcc tcttcgccgc cgccctgttg tggttcgtcg gggccgccgc cgccgcactt
      481 ccggccggcg tcaccgcagg cgcctcggtc gaaggcatca gcgagtacgc gctggccaac
      541 ggcctgaagg ttctgctgtt tcccgatgcc agccagccca aggtcaccgt caacatcact
      601 tatctcgtcg gatcgcggat ggagaactac ggcgagaccg gcatggcgca cctgctcgag
      661 cacatggtct tcaagggaac gaagacgcgc ggcaacctga tgaccggcct cgcgcagcgc
      721 ggcatggcgt tcaacggcac gacctggtac gaccgcacca actatttcga gaccttcccc
      781 gcatccgacg agagcctcga ctgggcgctg gggatggaag ccgaccgcat ggtcaactcg
      841 accgtgctgc gcaaggacct cgacagcgaa atgacggtgg tgcgcaacga aatggagcgc
      901 aacgaaaaca gcgccgtacg catcctgatc cagcgcacga ctgccgccgg cttcgactgg
      961 cacgcctacg gcaaggacac gataggcgcg cgcaccgacg tcgagcgcgt cgacatcggc
     1021 cggctgcagg ccttctatcg cctgtactac cagccggaca acgcggtgtt gaccatcgcc
     1081 ggggcgttcg atccggagcg cgcgctggcg ctggttgcaa agtatttcgg accgctgcca
     1141 aaaccgtcac gggttctgcc tgcgctgtac accgacgagc cggtgcagga tggcgcccgc
     1201 gaggtcacgc tgcgccgcgt cggcaatacg caatggcttt ccgcgatgta tcacaccgtg
     1261 cccggcgcgc atccggacgc cgtcgcgatc tcggccgcgg tcgacgcgat gaccgtcagt
     1321 cccggcgggc gcctttacaa ggcgctggtc gaagccaaaa aagccgccgc cgtcgacgac
     1381 ttcgtctaca ccggcttcga tcccggattt gcgatgtttc tcgcgcaggt gccggaaaac
     1441 gagtcgctgg acacggcgcg cacgacgatg ctgcaggtga tcgaggacgt cgcgaagcag
     1501 ccgtttacgc aaggcgaaat cgaccgcgtt cgggcgaagg cgctgaagtc gatcgacgag
     1561 acgatcaacg atccgcagcg cctgggcatc gcgctttcgg gatcgatcgc ggcgggcgac
     1621 tggcgcctgt tctttctgca gcgcgaccgc tggcgcgcgc tgactcccgc cgacgtcgat
     1681 cgcgtcgcgg gcgcgtattt caaggcggcc aatcggacgg tcggcgaatt cattcccgac
     1741 gccaagcccg accgcgcgcc ggcaccgcca accgtcaaca tcgcggcgat ggtcaaggac
     1801 tacaagggcg acccggcggt cgccgccggc gagactttcg acgcgacgcc cgccaatctc
     1861 gacgcgcgcg cgcaacgcta cacgctcgcc aacggcatga aggtcgcgct gcttccgaaa
     1921 aagacccgcg gcgagaccgt gcacttctat ttgtcgatgc attacggcga cgaggcgtcg
     1981 atcaagggcc gcgaaggcga ggctcgactt gccggtagca tgctgctgcg cggcacgtcg
     2041 aagcacagcc ggcaggagat cgaggacacg ctcgaccggc tgc
//
LOCUS       DATANS010000308         3525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_646900, whole genome shotgun sequence.
ACCESSION   DATANS010000308 DATANS010000000
VERSION     DATANS010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646900"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..938
                     /locus_tag="VHT22_04410"
     CDS             3..938
                     /locus_tag="VHT22_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX3136829.1"
                     /translation="MSASGLNSEAILRRLEWTVIRRLDGLLHGDYRTLFRGFGVDLAD
                     LREYEYHDDVRHIDWNVTARLQTPYVRVYNEERDITAWFVLDLSPSIDFGSQAVNKRA
                     VATELVTVLARMLTRHGNRVGALFYGDSVDTVIPARSGRRQVLHILHKMRGRPEVAQS
                     DATRLDELFRTAYQVIARRSLVFVVSDFISKPGWAKPLAQLAQRHEVIAVRLYDNLEM
                     DLPDFGLVVIQDAETGEQLHVDTGDRAFRRRFAAAAAKREIELRTAFRHAGIDALELA
                     TDDDLMDAILRFADMRKRRSRLASGGALPQHLEYR"
     gene            935..1987
                     /locus_tag="VHT22_04415"
     CDS             935..1987
                     /locus_tag="VHT22_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX3136830.1"
                     /translation="MTFLWPAMLWLGVLIPVVVVAYVLMQRRRKKQAMRYARLALVRN
                     AQGAAPRLRRHLPPVLMLLALALMIVAIARPASVISLPYQYETVILAIDASGSMRAAD
                     VAPTRIAAARAASRNFIAAQPRDTRIGVVAFAATASVVQTPTLNRDDILAALDRIQLQ
                     KGTAIGSAILVSLKLIFPDVEFDLRSANPRPGGAREAQRGTSLDPAPNAKAPADKPPP
                     GSFKSAAIVLLTDGQTTAGPDPFESAKMASERGVRVFTVGVGTPGGEVLAAEGWSMRV
                     RLDEDALKTIADTTKAEYFHAGTATDLSGIYQALTARLVFEKRATEITALFAAAAAVL
                     ALLSGLLSLLWFNRIL"
     gene            2012..2266
                     /locus_tag="VHT22_04420"
     CDS             2012..2266
                     /locus_tag="VHT22_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136831.1"
                     /translation="MASARPRRSTRKPRKHEVKADLANFELAKARSSLNLQIYADREK
                     IGELEIGRGSLYWYGRRKQKSKRIDWSQFAQMMDRLAYGE"
     gene            complement(2296..2922)
                     /locus_tag="VHT22_04425"
     CDS             complement(2296..2922)
                     /locus_tag="VHT22_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008054279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136832.1"
                     /translation="MAALACGLAFAASAGAADMSFFITSTGGGKGADLGGLAGADRHC
                     QALAAAAGAGGQTWHAYLSTTAAPGQPAINARDRIGAGPWQNAKGVVVAKNVAELHGD
                     NNVNKETALTEKGDIVNASGDTPNKHDILTGSQADGTAYPPGDDKTCHNWTSSSEGAV
                     QVGHHNRNGTGPAPVTYSWNSAHATPGCSDEALRKTGGAGLIYCFAVK"
     gene            3186..3437
                     /locus_tag="VHT22_04430"
     CDS             3186..3437
                     /locus_tag="VHT22_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136833.1"
                     /translation="MKLGAPSLVLAGLFLACSSAIAEAQAPNTAAPVAAATPPAVSAS
                     PVPTDRRRDAHADARVCLEFPTELQVIACAEKYRPARKR"
BASE COUNT          557 a         1205 c         1153 g          610 t
ORIGIN      
        1 ccatgtccgc gtcgggcctc aactctgagg cgatcctccg ccgtctcgag tggacggtca
       61 tccgccggct cgacgggctg ctgcacggcg actatcgcac gctgttccgc ggcttcggtg
      121 tcgacctggc cgatctgcgg gaatacgaat atcacgacga tgtgcgccat atcgactgga
      181 acgtgaccgc tcgcctgcag acgccttacg tccgcgtcta caacgaggaa cgcgacatca
      241 ccgcctggtt cgtgctggac ttgagcccgt cgatcgattt cggctcgcaa gcggtcaaca
      301 agcgcgccgt ggcaaccgag ctggtcaccg ttctggcgcg catgctgacc cggcacggga
      361 accgggtcgg cgcgctgttc tacggcgaca gcgtcgacac cgttatcccg gcgcgatcgg
      421 gccggcggca ggtgctccac atcctgcaca agatgcgggg ccgccccgag gtcgcgcaat
      481 cggacgcgac ccggctcgac gaactgttcc ggaccgccta ccaggtaatt gcccggcgct
      541 cgctggtctt tgtcgtatcg gacttcatca gcaagcccgg atgggctaaa ccgctggcgc
      601 agctcgcaca gcggcacgag gtcattgccg tgcgcctgta cgacaatctg gagatggacc
      661 tgcccgactt cggcctggtc gtcatccagg acgccgagac cggcgagcag ctgcacgtcg
      721 acaccggcga tcgcgcgttt cgccggcgct tcgcggccgc cgcggcgaag cgcgagatcg
      781 agctgcgcac ggcttttcgc cacgctggca tcgacgcact ggagctcgcg accgacgatg
      841 atctgatgga cgcgatcctt cgcttcgccg atatgcgcaa gcggcgcagc aggctcgctt
      901 ccgggggcgc cctgccccag cacctcgaat accgatgacg ttcctctggc cggcgatgct
      961 ttggctcggg gtgttaattc ccgtcgtcgt ggttgcgtat gtgctgatgc agcggcgccg
     1021 aaaaaaacag gcgatgcgct atgcccgcct cgcgctggtc aggaatgcac aaggcgcggc
     1081 accgcggctg cgccgccatc tgccgcccgt tctgatgctg ctcgcgctgg cgctgatgat
     1141 cgtcgcgatt gcgcgtccgg cgtcggtgat cagcctcccg tatcaatacg aaacggtgat
     1201 cctcgcgatc gacgcttcag gcagcatgcg cgctgccgac gttgccccga cgcggatcgc
     1261 ggcggcaagg gccgcgtcga ggaatttcat cgccgcgcag cctcgcgaca cgcggatcgg
     1321 cgtcgtggcg ttcgcagcga cggcatctgt cgtgcagacc ccgacgttga atcgcgacga
     1381 catcctcgct gcgctcgacc gcattcagct gcagaaaggc accgcgatcg gcagcgcgat
     1441 cctcgtttcc ttgaagctta tctttcccga tgtggaattc gacctgcgct cggccaatcc
     1501 gcggcccggc ggcgcacggg aagcccagcg cggcacgtcg ctcgacccgg cgccgaacgc
     1561 gaaggcgccc gcggacaagc cgccgccggg ctcgttcaag tccgctgcca tcgtcctgct
     1621 gaccgacggc cagacgaccg ccggcccgga tcctttcgaa tcggcgaaga tggcttccga
     1681 gcgtggcgtg cgcgtcttca ccgtcggcgt gggcacgccc ggcggcgagg ttctggccgc
     1741 ggaaggctgg tcgatgcgcg tgcgcctcga cgaagacgcg ctcaagacca tcgccgatac
     1801 gaccaaggcc gaatattttc atgccggcac ggcaaccgac ctttccggca tctaccaggc
     1861 gttgaccgcc cgcctcgtat tcgaaaaaag ggcgacggag atcaccgcgc tcttcgccgc
     1921 ggcggccgcc gtgctggcgc tgctgtcggg cctgttgtcg ctgctgtggt tcaaccgcat
     1981 cctgtgaagg tgcgcgccag aggagcacgc tatggccagc gctcgaccca gacgtagcac
     2041 gcgcaagccg cgcaagcacg aggtcaaggc ggacctggcc aacttcgagt tggccaaggc
     2101 gcgctcatcg ctgaatctgc agatctatgc cgaccgcgaa aaaatcggcg agctcgagat
     2161 cggccgcggc tcgctctact ggtacgggcg gcgcaagcaa aagagcaagc gcatcgactg
     2221 gtcgcagttc gcccagatga tggatcggct cgcgtacggc gagtaggccg cacgcgatcc
     2281 cggtccggca gcgccttact tcaccgcaaa acaatagatc aggccggcgc cgccggtctt
     2341 gcgcaaggcc tcgtcgctgc atccgggcgt cgcgtgcgcc gagttccacg aatacgttac
     2401 cggcgcgggg ccggtgccgt tgcggttgtg atgtccgacc tgaaccgcgc cttcgctgct
     2461 gctggtccag ttgtggcagg tcttgtcgtc gcccggcgga tacgcggtgc cgtcggcctg
     2521 cgatcctgtg aggatgtcat gcttgttggg cgtatcgcca ctggcattga cgatgtcgcc
     2581 tttttccgtc agcgccgttt ccttgttgac gttattgtcg ccatggagtt cggcgacgtt
     2641 cttggcgacg accacgccct tcgcattctg ccaaggcccg gcgccgatgc ggtcacgcgc
     2701 gttgatcgcg ggctgccccg gcgcggcggt ggtgctgagg taagcgtgcc aggtttggcc
     2761 gccggctcct gccgcggcgg ccagcgcctg gcaatgccgg tccgcacccg cgagtccacc
     2821 caagtcggca cccttgccgc cgccggtgct ggtgatgaaa aagctcatgt ccgctgcgcc
     2881 ggccgatgcg gcaaaggcca gaccgcatgc aagcgcggcc atcgtcgccg ccgggaaccg
     2941 ctcgttattc ttcatccgtt cctccgaaat gtgtttgttg cctgccatgc cagcgaatgc
     3001 cgcagcacgg accgcggcaa acactggcag cgtaaatgat attcccgttc ggcggccgcc
     3061 ggccgcagaa cgcaggccgg cgctggccag ccgggggtct ctggtgccac aatcacagcg
     3121 agacagggca acgggcagcg atgccgggac gtcgtcccga ccgcgcgccg caatggggag
     3181 gctccatgaa actcggtgcg ccatcgctgg tgctggcagg gttgtttctc gcgtgctcgt
     3241 cggcgattgc cgaagcccag gccccgaaca ccgcggcccc ggtagcggcg gctacgccgc
     3301 cggcggtatc cgcatcgcct gtcccgaccg accgccggcg cgacgcgcac gcggatgcgc
     3361 gcgtatgtct ggagtttccg accgagctcc aggtgatcgc gtgcgccgag aagtaccgtc
     3421 cggccaggaa gcgatagccg ccggcgtccg ccgtcagcga ggcccgcgcc cggaatgacc
     3481 gcgccattga cgtcccgctc gcgcttttcg ctgcaaagct tcgag
//
LOCUS       DATANS010000309         4869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_225217, whole genome shotgun sequence.
ACCESSION   DATANS010000309 DATANS010000000
VERSION     DATANS010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225217"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..635)
                     /gene="clpP"
                     /locus_tag="VHT22_04435"
     CDS             complement(15..635)
                     /gene="clpP"
                     /locus_tag="VHT22_04435"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX3136834.1"
                     /translation="MTSAGRWEEPKALGLIPMVIEQSGRGERAYDIYSRLLKERVVFL
                     IGPVDDATANLVVAQMLFLESENPDKDINFYINSPGGSISAGMAIYDTMQFIKPDVST
                     LCVGMAASMGAFLLAAGAKGKRFALPNSTVLIHQPLGGFQGQASDIAIHAKYILSLRE
                     RLNRLLAEHTGKPIGQIEKDTERDNFLTADESLSYGLIDKVLQSRA"
     gene            complement(681..1988)
                     /gene="tig"
                     /locus_tag="VHT22_04440"
     CDS             complement(681..1988)
                     /gene="tig"
                     /locus_tag="VHT22_04440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX3136835.1"
                     /translation="MQTTLETLGQLERRLNVAVPREQIDGEVHKRLARLAKTVKIAGF
                     RPGKVPLKMVAQQYGPQVRSDVISDTVQASLNDAIREQNLRVAGYPRIEPKQDAAGDQ
                     LEFSAVFEVYPEIKLGELSGVTIERPVSEVAPVDVDRTIEILRQQNVRYEGVARPAAH
                     GDRAVVDFTGTIDGVAFPGGQAKDFPIVIGEGRMLPEFESALTGMASGETKTFGLTFP
                     ADYHGKEVAGKAAEFTMTVQSVSEPKLPQVDSEFAVKFGIASGSVEELRAEIAANLAL
                     ELKRKIEGVLREQVLRALKSTSDLIVPRSLVELEAQRLLERAVSDLQSRGVKAEDMQL
                     SPDTFRPQAEERVTYGLIVNEITRVYQLSPKPEQVLALVREAAQAYEQPEAVVRWHYE
                     KPGRLNEFEAQAVEQNVVDWALQRARVEDRPTSFATLMEPPAR"
     gene            complement(2026..2110)
                     /locus_tag="VHT22_04445"
     tRNA            complement(2026..2110)
                     /locus_tag="VHT22_04445"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2074..2076),aa:Leu,seq:tag)
     gene            2237..3058
                     /gene="hpnC"
                     /locus_tag="VHT22_04450"
     CDS             2237..3058
                     /gene="hpnC"
                     /locus_tag="VHT22_04450"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEX3136836.1"
                     /translation="MPVSHYENFPVASWVGPPALRAAISAIYGFARGADDIADEGDDP
                     PAVRLAGLDLYARMLDRIESSSDARVEPAGPPFEALAEAIRRHSLPIEPFRDLLSAFR
                     QDITKPRYADIDELFDYCRRSANPIGRLLLHLYGVSAEVELGRADAICTGLQLANFWQ
                     DIALDWAKGRVYLPADDMARFGVSERHIADAIADERWRRLMAFEVARTRELLRAGLPL
                     ANALPLRARLELKMVVAGGLRILRAVDLAQGDVFRRRPVLRLRDWAMMFAATLGG"
     gene            3061..3894
                     /gene="hpnD"
                     /locus_tag="VHT22_04455"
     CDS             3061..3894
                     /gene="hpnD"
                     /locus_tag="VHT22_04455"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEX3136837.1"
                     /translation="MHPDVYCQQKAAQSGSSFYRSFRFLPAARRRAITALYAFCREVD
                     DVVDDTTDVGVARIKLAWWRGQVAAIFGGAPQHPVAQALRPIVAEFALKEAQLQAVID
                     GMAMDLEQQRYLDFAMLERYCDLVAGGVGLMSAEIFGYSDPATCAYARDLGIAFQLTN
                     IIRDVGEDARRGRIYLPQDELDRFGVSAADILGARHTPGFVALMRFQVARARATYARA
                     LGALPAADRRAQRPGLIMAATYRALLRAIERNDFQVLDRRVALSPLRKAWIAWTTSWS
                     Y"
     gene            3861..>4869
                     /gene="hpnE"
                     /locus_tag="VHT22_04460"
     CDS             3861..>4869
                     /gene="hpnE"
                     /locus_tag="VHT22_04460"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HEX3136838.1"
                     /translation="MDRLDDVLVVLVAERIAVIGGGYAGFAAAVTLAQAGRAVTVFEA
                     AQTLGGRARRIEAYEVVVDNGEHMLLGAYTELLALLRTVHGPGAERDLLERRRLCLAE
                     PGVFRLAAPRLPAPWHLALALLRAQGLTWPERRTTLAFVGRLRRDRYRCSRAMTVAEL
                     LDGQPQAAIRNLWEPLCIAALNTPLAVASAQIFLNVLAAAFAREAHDSDTLMPRVDLS
                     SLFPDAAAAYVVDRGGSIRKGTTVTGVAPRGDHVVLRSGYTDESFGDAVVAVGPHQLG
                     HLLGGAIDSPEAAHAVACVEQFAYEPIVTAYLKYDRPLGLAQPMQKLAGTPGQWLFDR
                     GQ"
BASE COUNT          734 a         1632 c         1574 g          929 t
ORIGIN      
        1 acactgtacc cggcctaagc ccggctctgc aacactttat cgatcagccc gtaggacagg
       61 gactcgtccg ccgtcaggaa attatctcgc tcggtatcct tctcgatctg cccgatcggc
      121 ttgccggtgt gctcggccag caagcggttg agccgctcac gcaggctcag gatgtacttg
      181 gcgtgtatcg caatatccga tgcctggccc tggaagccgc ccaacggctg gtggatcaga
      241 acggtcgaat tgggcagcgc gaagcgcttg cccttggcgc ctgccgccag caggaaggcc
      301 cccatgctgg ccgccatgcc gacgcatagc gtagacacgt ccggcttgat gaactgcatc
      361 gtgtcgtaga tcgccatgcc ggccgaaatc gatccgcccg gcgaattgat gtaaaaattg
      421 atgtccttgt cgggattctc cgactcgagg aacagcatct gcgcgacgac cagattcgcc
      481 gtcgcgtcgt cgaccggccc gatcaggaat acgacgcgct ccttcagcag tcgcgagtag
      541 atgtcgtagg cgcgttcacc gcgcccgctt tgctcgatca ccatcgggat caagcccagc
      601 gccttcggtt cctcccacct gcctgcactc gtcatcggtt ggcctgtcgt cgattaaaag
      661 gtttcgtccg caccgcgcgt tcagcgcgcc ggcggctcca tcagtgtcgc gaagctcgtc
      721 ggtcggtctt cgacgcgagc gcgttgcagc gcccagtcga cgacgttttg ttcgactgcc
      781 tgcgcttcga attcgttcag gcgcccgggc ttttcgtagt gccagcgcac taccgcttcc
      841 ggttgctcgt aggcctgcgc cgcctcgcgc accagggcca gcacctgttc gggcttgggc
      901 gagagctggt agacccgggt gatctcgttg acgatcaggc cgtaggtcac ccgctcctcc
      961 gcctgggggc gaaacgtgtc gggcgagagc tgcatgtctt cggccttgac tccccggctc
     1021 tgcaagtcgg agaccgcccg ctcgagcagc cgctgcgctt ccagctcgac cagcgatctg
     1081 ggcacgatca ggtcggaagt tgatttgagc gcccgaagga cctgctcgcg aagcacgcct
     1141 tcgatcttcc gcttgagctc tagcgcgaga ttggcggcta tttcggcgcg caactcttcg
     1201 acgctgccgc tggcgatgcc gaatttgact gcgaactcgg aatcgacttg aggaagcttc
     1261 ggttcgctga ccgattgcac ggtcatcgtg aattccgccg cttttccggc gacttccttg
     1321 ccgtggtaat cggcggggaa agtcaggccg aatgtcttgg tctcacccga cgccattccg
     1381 gtcagcgcgc tttcgaactc cggcagcatg cggccttcgc cgatgacgat cggaaagtct
     1441 ttcgcctggc cgccgggaaa tgccacgccg tctatggtgc cggtgaaatc gacgaccgcg
     1501 cggtcgccgt gcgcggccgg gcgtgcgacc ccctcgtagc gaacgttctg ctggcgcagg
     1561 atctctatcg tccggtcgac gtctaccggg gcgacttccg acaccgggcg ctcgatcgtc
     1621 acgccggaca attcgccgag cttgatttcc gggtaaacct cgaagacagc ggagaactcg
     1681 agctgatccc cggccgcgtc ctgcttcggc tcgatccgcg gatagcccgc cacgcgcagg
     1741 ttctgctcgc gaatggcgtc gttgaggctc gcctggacgg tgtcggagat cacgtcggag
     1801 cgtacttgcg ggccgtactg ctgcgccacc attttcagcg gaaccttgcc cggccgaaaa
     1861 cccgcgatct tgaccgtctt cgcaaggcgc gccagccgct tgtgcacttc gccgtcgatc
     1921 tgctcccgcg gcacggccac attaagccgg cgctcgagct ggcccagcgt ttccagtgtc
     1981 gtttgcatac ccgatgattc cttgctagcc ttaatgttgt tttcgtggtg cgaaaggaga
     2041 gactcgaact ctcacgcctt tcggctccgg ctcctaaggc cggcgcgtct accaattccg
     2101 ccactttcgc agccggtttc gcgaaacgcg gcagtatagc cgaaaagtcc cacccccgcc
     2161 acgcgaaaga gtccggccgg cgccgtgttt cccggaacaa tttgtgaacg tcctcgtgca
     2221 acaagctccg atcgccgtgc ctgtcagcca ctacgaaaat tttcccgtcg catcatgggt
     2281 gggccctccg gcgcttcgcg cggcgatttc ggcgatctat ggtttcgccc gcggcgcgga
     2341 tgacatcgcc gacgaggggg acgatccgcc ggctgtccgc ctcgccggcc tcgatcttta
     2401 tgcaaggatg ctcgaccgca tcgagagctc gtccgatgcg cgcgtcgaac cggcggggcc
     2461 gccgttcgag gcgctggccg aggcgatacg ccgccattcg cttccgatcg agccgtttcg
     2521 cgatctcttg tcggcgtttc gccaggacat aaccaagccg cggtacgcgg acatcgacga
     2581 gcttttcgac tattgccggc gctcagccaa cccgatcggt cgcttgctgc tgcatctgta
     2641 cggcgtctcg gccgaggtcg agctcggccg agccgatgcg atctgcaccg gattgcaact
     2701 ggccaatttc tggcaggaca tcgcactcga ctgggcgaaa gggcgcgtct acctgccggc
     2761 cgacgacatg gcgcgcttcg gcgtaagcga gcggcacatc gccgacgcga tcgctgacga
     2821 gcgctggcgc cgcctgatgg ccttcgaggt ggcgcggacg cgcgagctgc tgcgcgcggg
     2881 actgccgctc gcgaacgcgt tgccgctgcg cgcgcggctc gagctgaaga tggtggtcgc
     2941 cggcgggttg cgcatcctgc gggcagtcga cctcgcgcag ggagacgtct tccggcgccg
     3001 tccggtgttg cgcctgcggg actgggcgat gatgtttgcg gcgacgctgg gcggttagcg
     3061 atgcaccccg atgtctattg ccagcagaag gccgcacaaa gcggctcgag cttctatcgg
     3121 agctttcgct ttctgccggc ggctcgccgg cgcgcgatca ctgcgctgta tgcgttctgc
     3181 cgcgaagtcg acgacgtggt cgatgacacc accgatgtcg gcgtggcgcg catcaagctt
     3241 gcctggtggc gcggccaggt ggcggcgatt ttcggcggcg caccgcagca cccggtagcg
     3301 caggccttgc ggccgattgt tgcggagttc gcactcaagg aagcgcagct gcaggccgtc
     3361 atcgacggca tggcgatgga tctcgagcag cagcgctatc tcgactttgc gatgctcgaa
     3421 cgctattgcg accttgtcgc cggcggcgtc ggactgatgt cggcggaaat attcggttat
     3481 tccgatccgg cgacgtgcgc ttacgctcga gatctcggca tcgcatttca gctgacgaac
     3541 atcatccgcg acgtcggcga ggatgcgcgc cgcggtcgga tctatctgcc gcaggacgag
     3601 ctcgatcgct tcggcgtcag cgcggccgac atactcggcg cacgccacac acccggattt
     3661 gtcgcgctga tgcgtttcca ggtcgcgcgt gcgcgcgcca cttacgcgcg ggcgctcggc
     3721 gcactgcccg ccgccgaccg gcgcgcgcaa cgtcccggtc tgatcatggc ggccacctat
     3781 cgcgcgctgc tcagggcgat cgagcgcaac gactttcagg tcctggaccg ccgcgtcgcg
     3841 ctgtcaccgc tgcgcaaggc atggatcgcc tggacgacgt cctggtcgta ttagtggcgg
     3901 agcgaatcgc ggtcatcggt ggcggctacg cgggcttcgc ggcggcagtg acgcttgccc
     3961 aagcggggcg cgcggtcacg gtgttcgaag cggcgcagac gctgggcggc cgcgcccgtc
     4021 gaatcgaagc ctacgaggtc gtcgtcgaca acggcgagca catgttgctc ggcgcgtaca
     4081 cagagctcct cgctctgctg cgcaccgtgc acggccccgg cgccgagcgc gatctgctcg
     4141 agcggcggcg gctgtgcctg gccgagcccg gcgtgttccg tctggccgcg ccgcggctcc
     4201 ccgccccctg gcacctcgcg ctggcgctgt tgcgcgcaca aggccttacc tggcccgagc
     4261 gtcgcacaac gctcgcgttc gtcggtcgac tgcggcgcga ccggtatcgc tgttcgcggg
     4321 caatgacggt ggcggagctc ctcgatggac agcctcaggc agcgatacga aatttgtggg
     4381 agccgctgtg catcgccgcg ctcaatacgc cgctggccgt cgcgtcggcg cagatctttc
     4441 tcaacgtgct cgcggccgcg ttcgcccgcg aggcccacga ttcggacacg ctgatgccgc
     4501 gggtcgacct gtcgtcgctg tttcccgacg ccgcggccgc ctacgtcgtc gaccgcggcg
     4561 gctcgatccg caagggtacg accgtgaccg gcgtcgcgcc acgcggcgac cacgtcgtgt
     4621 tgcgcagcgg ctataccgac gagtcgttcg gggacgcagt cgtcgccgtg ggcccgcacc
     4681 agctcggcca tctgctcggc ggtgcgatcg attctcccga ggccgcccac gcggtcgcgt
     4741 gtgtcgagca gttcgcctac gagccgattg tcaccgcgta tctgaaatac gatcgtccgc
     4801 tggggctcgc gcaaccgatg cagaagctcg ccggcacgcc ggggcagtgg ctgttcgatc
     4861 gcgggcagc
//
LOCUS       DATANS010000310         2771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_15369, whole genome shotgun sequence.
ACCESSION   DATANS010000310 DATANS010000000
VERSION     DATANS010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15369"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VHT22_04465"
     CDS             <1..843
                     /locus_tag="VHT22_04465"
                     /inference="COORDINATES: protein motif:HMM:NF040132.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136839.1"
                     /translation="AFLFFPTMDMNDMDMMMMMMGGSEMPDPYYALLYQSPAINGPAV
                     SAGASLSIGAPAQWYTMVPAVADHTTIMAHVTTFTGANAGGAVAASSAMTWDCTTGQV
                     LAMSNAGNADGASLSPAPGLVQVVEYYNAATDHYFLTSGSAEIAALDAGQIAGWVRTG
                     NSFNAFVDAVAGTSPVCRLYLPAIYGNSHFVSASPAECAYVQAAMPAAVLESANAFYV
                     DTPDTTTGACPSGTVAVYRLWNHRADLDHRYTTSATIKAAMIAQGYVAEGYGPDQVAM
                     CSPQ"
     gene            complement(917..992)
                     /locus_tag="VHT22_04470"
     tRNA            complement(917..992)
                     /locus_tag="VHT22_04470"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(956..958),aa:Glu,seq:ttc)
     gene            complement(1017..1092)
                     /locus_tag="VHT22_04475"
     tRNA            complement(1017..1092)
                     /locus_tag="VHT22_04475"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1057..1059),aa:Ala,seq:ggc)
     gene            1234..2007
                     /locus_tag="VHT22_04480"
     CDS             1234..2007
                     /locus_tag="VHT22_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3136840.1"
                     /translation="MPLPSLDYAPRRLLDGRVALVTGASGGLGRVLSLACAAQGATVV
                     LHGRVVRKLEALYDDIVNAGYPEPTILPLDLATARAEDFANVASALQAQHGRVDAIVH
                     TAVQLGSLGPIEHQAFDAWLAILRVDLLAPFGLTRALLPLLRAAPEGSVAFTLDTRGE
                     DPKAFWGAYAVAKAGLSALLAILADEWEDAPNLRVNGIVPGPMRSPLRAQTHPGDDIT
                     RLPPPEVFVPLYLHLLAGQPKAESGKVIDGQAWLRGAPA"
     gene            complement(2017..2664)
                     /locus_tag="VHT22_04485"
     CDS             complement(2017..2664)
                     /locus_tag="VHT22_04485"
                     /inference="COORDINATES: protein motif:HMM:NF018908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HEX3136841.1"
                     /translation="MAGRAEDRKHVAQSGHRRRNLARAGQASALRRRSSRHRPTRCAT
                     QSSGHRPFGRLTGARASVPNRYVKSVPVLCVTLQPSRIAARLLASMTIATAAMLALLP
                     GEPRLRGACVLIVGACGIRALRGTAASGNKGPIAAVDLAAGRRAILTDRSGRRIEAIV
                     QAESHVGALVTTLVLRPEGARRSRALAIWPDTMPAGEFRRLRMLLRHGDPGSAQT"
     gene            complement(2574..>2771)
                     /locus_tag="VHT22_04490"
     CDS             complement(2574..>2771)
                     /locus_tag="VHT22_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010633585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX3136842.1"
                     /translation="DAVKAMLAMAITACRKAGKYAGICGQGPSDHPDLAQWLVEQKIE
                     SMSLNPDTVVETWLALAKQAR"
BASE COUNT          455 a          926 c          901 g          489 t
ORIGIN      
        1 gctttccttt tctttcccac catggacatg aacgacatgg acatgatgat gatgatgatg
       61 ggcggcagcg agatgcccga tccgtactac gccttgctct atcagtcgcc cgccatcaat
      121 ggtccggcgg tgagcgccgg cgccagcttg agcatcggag cgcccgcgca gtggtacacg
      181 atggtccccg cggtggccga ccatacgacg atcatggcgc acgtaacgac gttcaccgga
      241 gcgaacgccg gcggcgccgt ggctgcttcg tcggcgatga cctgggactg cacgaccggg
      301 caagtgctcg ccatgtcgaa tgcgggcaat gcagacgggg caagcctttc ccctgcgccc
      361 gggctggttc aggtggtcga atactataac gcggccactg atcactactt cctgacgtcg
      421 ggcagcgccg aaatcgccgc attggatgcc gggcagatcg ccggctgggt acgtaccgga
      481 aacagcttca atgcattcgt cgacgccgtc gccggcacga gccccgtgtg ccggctttac
      541 ctgcccgcca tctacggcaa ttcgcatttc gtctccgcgt cgccagctga gtgcgcgtac
      601 gtgcaggcgg cgatgcccgc ggccgtgctc gaatcggcga atgccttcta tgtcgatacg
      661 ccggatacca cgacgggcgc ctgtccctcg ggcacggtcg ccgtgtatcg gctgtggaac
      721 catcgcgccg acctggatca tcgttacacg accagcgcga cgatcaaggc agcgatgatc
      781 gcgcaagggt acgttgccga gggctacggg ccggatcagg tcgcgatgtg ctcgccgcaa
      841 taagcatcgc gtcgcagccg tggcgcatgg cggctccgcg gacccgagct tccggaatga
      901 gagaagcgtg cgatgttggc gtccccagcg agattcgaac tcgcgtcgtc gccgtgaaag
      961 ggcgatgtcc taggcctcta gacgatgggg acgaggcgga tgttgcgcag ggctggtggt
     1021 ggagctaagc gggatcgaac cgctgacctc ttgcatgcca tgcaagcgct ctcccagctg
     1081 agctatagcc ccacagcgaa ccgcgaatta taagggccgg taccgcgttt tgcaaacgcg
     1141 agcgccccgt cgtcgtttgt ctctggcatc gtcattcgac agccgctgcg gtcttttcgg
     1201 atattatcgg cgcgccccgc acgcaccgtt tcgatgccgc taccttccct cgattacgca
     1261 ccccgccgac tgctcgacgg ccgggtggca ctcgttaccg gtgcaagcgg cggactggga
     1321 cgagtgctct ctctcgcgtg cgcggctcag ggagcgaccg tcgtcctgca cggacgtgtg
     1381 gtgcgcaagc tggaagcgct ctatgacgac atcgtcaacg ccggataccc ggagcccacg
     1441 atcctgccgc tcgacctcgc gaccgcgagg gccgaggact tcgccaatgt cgcgagtgcg
     1501 ctgcaggctc agcacgggcg agtcgacgcg atcgtgcaca ccgccgtgca gctgggctcg
     1561 ctcggtccga tcgagcacca ggcgttcgat gcctggctcg cgatactgcg ggtcgatctg
     1621 ctcgcgccgt tcgggctgac tcgcgcgctt ctgccgctgc tgcgcgccgc gcccgaaggc
     1681 agcgtcgctt tcacgctgga cacgcgcggc gaagatccga aggcattctg gggtgcctat
     1741 gcggtcgcca aggcaggtct gtccgccctg cttgcgatcc ttgccgacga atgggaagac
     1801 gcgccgaatc tgcgggtcaa cggcatcgtc cctggtccga tgcgctcgcc gctgcgggca
     1861 cagacccatc cgggcgacga catcacacgc ctgccgccgc cggaagtctt tgtgccgctt
     1921 tatctgcact tgctggccgg tcagccgaaa gccgaaagcg gcaaggtcat cgacggacag
     1981 gcgtggctgc gaggcgcgcc cgcgtagcgg gccacgtcag gtttgggcgc ttccgggatc
     2041 gccgtgccgc aacagcattc gcagacgcct gaattcaccg gctggcatcg tatccggcca
     2101 gatcgccagt gcgcgcgagc gccgcgcacc ctcggggcgg agcaccagcg tcgtcaccag
     2161 ggcgccgaca tggctttccg cctggacgat cgcttcgata cgccggccgg atcgatcggt
     2221 gaggatcgcg cgtcggccgg ccgcaagatc gacggccgcg atcggaccct tgttgccgct
     2281 cgccgccgtg ccccggagcg cacgaatccc gcaggcaccc acgatcagga cacatgcgcc
     2341 gcgcagccgg ggctctccgg gcaacagcgc gagcattgcc gcggttgcga tcgtcatcga
     2401 agcgagtagt cgcgcggcaa tgcgcgatgg ttgaagcgta acgcacagga ccggcaccga
     2461 tttcacgtat cgattgggca cgctagcgcg cgctccggtc aatcgtccga atggccgatg
     2521 accgctgctc tgcgttgcac aacgcgtcgg ccgatgtcgc gacgaccgcc gcctcagcgc
     2581 gcttgcttgg ccagcgcgag ccaggtttcg acgacggtgt ccggattgag cgacatgctt
     2641 tcgatcttct gctcgaccag ccactgcgcg agatcgggat ggtcggatgg cccctgtccg
     2701 cagatgccgg cgtacttccc cgccttgcgg cacgctgtga tcgccatcgc cagcatcgcc
     2761 ttgaccgcat c
//
LOCUS       DATANS010000311         1683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_520082, whole genome shotgun sequence.
ACCESSION   DATANS010000311 DATANS010000000
VERSION     DATANS010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520082"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /locus_tag="VHT22_04495"
     CDS             complement(<1..1589)
                     /locus_tag="VHT22_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic beta 1-2 glucan synthetase"
                     /protein_id="HEX3136843.1"
                     /translation="MTGRLRSQPTNRKYTSDEPPLRAELFSADQMEQHGKVLAGVHRV
                     SVGRAPDHLLARLSENETALLQCCKAITGAVKANRRISPAGEWLLDNFYLIEEQIRTA
                     KRHLPKGYSRELPRLARGPSAGLPRAYDIALEAISHGDGWVDPGSLARFVAAYQKVTP
                     LKLGELWAIPIMLRLALIENLRRVGQRVAASTRDRERADAWADQMMAVAEQDPKSLIL
                     IVADMARSNPPMVSSFVAELARRLQGQSAALALPLTWIEQRLSELGLTIEQLVQSETQ
                     KQASAQVSISNSIGSLRLLGAMDWREFVESLSGVEQKLREDPYGIYSTMDFATRDHYR
                     HAVEKLAKATRLSEVDVARKAIQLANAAAGTPATQSNDAARAAHVGYYLIDRGLPQLK
                     QAAEVHPSFGGSVLEVASRFPLPLYLGAIALLTAAFTAGLVAMAPAELSGAWLALAGA
                     LAAVCASQFAIALVNWFATLIATPRPLPRLDFSRGIPPACRTLVVVPTMLTGVENVDD
                     LIEALEVRFLANRDEHLHFGLL"
BASE COUNT          280 a          567 c          555 g          281 t
ORIGIN      
        1 agcagcccga aatgcagatg ctcgtcgcga ttggcaagaa aacgcacttc gagcgcctca
       61 atgaggtcgt cgacgttttc cacacccgtc aacatcgtcg gcacgacgac cagcgtgcga
      121 cacgcgggcg ggatgccgcg cgagaagtcc aggcgcggca acgggcgcgg cgttgcaatc
      181 agcgtggcaa accagttgac gagtgcgatc gcgaactggc tcgcgcacac cgccgccagc
      241 gcgccggcca gcgcgagcca tgcaccggag agctcagccg gcgccatggc gaccagcccg
      301 gccgtgaaag cagccgtcaa cagcgcaatc gcgcccagat aaagcggcag gggaaaccgg
      361 ctggccacct ccagcacgct gccgccgaac gacggatgca cttcggcagc ctgcttcaat
      421 tggggcaggc cgcggtcgat caggtagtag ccgacgtgcg ccgcgcgcgc ggcgtcgttg
      481 ctctgcgttg cgggcgtgcc ggcagcggcg ttcgccagct ggatggcctt gcgtgcgacg
      541 tcgacctccg agagccgggt cgcctttgcc agtttctcca ccgcgtgccg gtaatggtcg
      601 cgcgtggcga agtccatcgt ggagtaaatg ccgtacggat cctcgcgcag cttctgctcg
      661 acgccgctca gcgattcgac gaattcgcgc cagtccattg cgccaagcag gcgcaggctg
      721 cctatgctgt tcgagatcga gacctgcgcg gacgcctgct tttgcgtttc cgattgcacg
      781 agctgctcga tggtgaggcc cagctcggag aggcgctgct cgatccaggt cagcggcagc
      841 gcgagcgccg cgctctgtcc ctgcagccgg cgcgccagtt ccgcgacgaa tgagctcacc
      901 atcggcgggt tggagcgcgc catgtcggcg acgatgagaa tcaggctttt tggatcctgc
      961 tcggcgaccg ccatcatctg gtccgcccag gcgtcggcgc gttcgcggtc gcgggtgctc
     1021 gccgccacgc gctggcccac gcggcgcaga ttttcgatca gcgccaagcg cagcatgatg
     1081 ggaatggccc acagctcgcc caacttcaac ggagttactt tctggtaggc ggcgacgaac
     1141 cgcgcgaggc tgccgggatc gacccagccg tcgccgtgcg agatcgcttc gagcgcgatg
     1201 tcataagcgc gcggcagtcc cgccgacggc ccacgcgcca gccgcggcag ctcgcggcta
     1261 taacccttgg gcaggtgacg cttggcggtg cggatctgct cttcaatcag gtaaaagtta
     1321 tcgagcagcc actcgccggc cggcgaaata cggcggttcg ccttgaccgc tccggtgatc
     1381 gccttgcagc actggagcag cgcggtttcg ttttcggaca gccgcgccag caggtggtcg
     1441 ggcgcgcggc ccacgctcac acgatgcacg ccggccagca ccttgccgtg ctgctccatc
     1501 tggtcggcgc tgaacaactc cgcccgcagc gggggctcgt cgctcgtgta cttgcggttc
     1561 gtcggttggc tgcgcaggcg ccccgtcagc cggtgccatg catcgcgaaa aaagtcactg
     1621 cgcaaattca attcggcttt ccttgatgat cggcacgccg cggcttccac ggctaccgca
     1681 gta
//
LOCUS       DATANS010000312         2286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_225335, whole genome shotgun sequence.
ACCESSION   DATANS010000312 DATANS010000000
VERSION     DATANS010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225335"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            536..1102
                     /locus_tag="VHT22_04500"
     CDS             536..1102
                     /locus_tag="VHT22_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HEX3136844.1"
                     /translation="MLSGRRLDLLDPSALDVEIEDIAHGLARVARWNGQTSGQHIFSV
                     AQHTLLVEAIARRRTSLDRRARLALLLHDAPEYVIGDMITPFKAVIGDAYKAMERRLL
                     AAIHLRFGLPAVLPAELTKLIKSADRAAAYLEATRLAGFGETEAQRFFGNPTEVPAKL
                     ERDYLTPWNADDAKARYLKRFADSLKSE"
     gene            1129..1635
                     /locus_tag="VHT22_04505"
     CDS             1129..1635
                     /locus_tag="VHT22_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HEX3136845.1"
                     /translation="MIHVCSLARLHDTVRETGARHVVTLIKDIALVHRPASITPENHL
                     LLDMDDINEHIDGYVLPAEQHVGDLLRFVRAWPREAPLVVHCYAGISRSTAGAFVTAC
                     ALNPGRDEMMIARALREASPTASPNRRIVSLADRMLGRNGRMVLAIESIGPGMAAYAS
                     EPFRLDLE"
     gene            1638..>2286
                     /locus_tag="VHT22_04510"
     CDS             1638..>2286
                     /locus_tag="VHT22_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD regulator"
                     /protein_id="HEX3136846.1"
                     /translation="MAETALTPIEIGLTAAIVGVEAGEPAILVAGGDGSEVSAGLPFG
                     PFDPLAHRTFEIGLRAWVEQQTGLRVGYVEQLYTFGDRGRHAGPADVAHMVSVGYLAL
                     TRIPSASALKTSGAGFEPWYRFFPWEDWREKRPAILNDTIIPLLDQWTGTPHGELGRA
                     LGWRERLRLCFGVGGSPWDEEKVLDRYEMLYEAGLIEEARRDGRAAALARDRLPAL"
BASE COUNT          389 a          737 c          727 g          433 t
ORIGIN      
        1 tgcctccgat cgcacgcatc acgcccgatg ctttcgccga ggcaaagccc gacaattcgt
       61 tgagcttccc ttccaaatag gcgcgctgct ggttcacctt ttggttcaaa agcccgtgca
      121 ggcgatcgat atattgccga agctggccga catccatttt atccggagat ttggagcccg
      181 gtggccggcc ccgccgcttg gccttccttg ccatgattga cctttcaggt tgtggatgtg
      241 ctcgatgaat agcggatgcg gcaaatatga ggagatacct ggttggactt tagccagcga
      301 aatatcggcc gcatcaagct gacgcatttc atatcctttc catcccgcga agcaaagcaa
      361 cccagcattg tcgcatgcct gttttcgcgg tttacagagg gttatcgtgg gtctaatcgt
      421 gtcagcgggc gctaatcctg atttatttac tgtcctgcaa aaacggcaaa tctgataccc
      481 tctcactgtg ccaggcgacc gcgcatatcg acctcggccg cgcgcctggc agcgtatgct
      541 gtcgggccgc cggctcgatc tgcttgaccc atcggcgctc gacgtcgaga tcgaggatat
      601 cgcccacggg ctggcccggg tggcgcgctg gaacgggcaa acctcagggc aacatatttt
      661 ttcggtggcg cagcacacgc tgctggtcga agcgattgcg cggcggcgca ccagcctcga
      721 ccgtcgtgcg cggctcgcgc tgctgctgca cgatgcgccc gaatacgtga tcggcgacat
      781 gatcacgccg ttcaaggccg tgatcggcga cgcctacaag gccatggaac ggcgcttgct
      841 cgccgccatt catttgcgct tcggcttgcc ggccgtgttg cccgccgagc tcacgaagct
      901 gatcaagagc gcggatcgcg ctgccgccta tctcgaggcg acgcggcttg cgggatttgg
      961 cgagacggaa gcacagcgtt tttttggcaa tccgacggaa gtgcccgcga aactcgagcg
     1021 ggattacctc acgccgtgga acgccgacga cgcaaaggca cgttatctca agcggtttgc
     1081 ggactcgctg aaaagcgaat aactcaccgg tctcaccatg agagggtaat gattcacgtc
     1141 tgttccctcg cccgcctcca cgacaccgtg cgcgaaaccg gcgcgcggca tgtcgtaacc
     1201 ttgatcaagg acatcgcgct ggtgcaccgg cccgcgagca tcacgccgga aaatcatctg
     1261 ctgctcgaca tggacgacat caacgagcac atcgacggct atgtgctgcc ggccgagcag
     1321 cacgtcggcg atctgttgcg cttcgtgcgc gcgtggccgc gtgaggcacc cctggtggtg
     1381 cattgctacg caggcatcag ccgctcgacc gccggagcct tcgtcaccgc ctgcgcgctt
     1441 aatccaggcc gcgacgagat gatgatcgca cgcgctctgc gcgaagcctc gcccactgcc
     1501 tctccgaacc ggcgcatcgt ttcgctcgcg gatcgtatgc tcgggcgcaa cggccgcatg
     1561 gtcttggcga tcgagtcgat cgggccgggc atggcggctt acgcaagcga gccgttccgg
     1621 ctcgacctcg aatagcgatg gcggaaaccg ctctcacgcc gatcgaaatc gggctgaccg
     1681 ccgcgattgt cggcgtcgag gccggtgagc cggcgatcct ggtggcgggc ggcgatgggt
     1741 cggaggtgtc cgccggtctc ccgtttgggc cgttcgatcc gttggcgcat cgcaccttcg
     1801 agatcggcct gcgcgcctgg gtcgagcagc agaccgggct ccgcgtcggc tacgtcgagc
     1861 agctctacac gttcggcgac cgcggccgcc acgcgggccc ggccgacgtc gcgcatatgg
     1921 tctccgtcgg ctatctggcg ctcacgcgga tcccgagcgc gagtgcgctg aagacgagcg
     1981 gagccggttt cgagccctgg tatcgcttct ttccctggga ggactggcgc gagaagcggc
     2041 ccgcgatcct caacgacacg atcatcccgt tgctcgatca gtggaccggc acgccgcacg
     2101 gcgagctcgg ccgcgcactc ggctggcgcg agcggctgcg cctttgtttc ggtgtcggcg
     2161 gctcgccatg ggacgaggag aaggtgctcg accgctacga gatgctttac gaggccgggc
     2221 tcatcgagga ggcacggcgc gacggacgcg ccgcggcgct cgcgcgcgac agactgccgg
     2281 cgctcg
//
LOCUS       DATANS010000313         1634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_352144, whole genome shotgun sequence.
ACCESSION   DATANS010000313 DATANS010000000
VERSION     DATANS010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352144"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VHT22_04515"
     CDS             complement(<1..822)
                     /locus_tag="VHT22_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3136847.1"
                     /translation="MTAVPALDTPLLQVAGLVKHFPLRKGFFRRDARVVRAVDGIDFD
                     LAAGETLALVGESGCGKSTTGRLLLRLIEPTAGSVRFDGQELFALDAEAMRAQRRALQ
                     IIFQDPYASLNPRMTVAQTVGEPLALYGLASGRRSDRVAELLGLVGLAPQHTERYPHE
                     FSGGQRQRIGIARALAVEPRLIVCDEPVSALDVSIQAQVINLLMDLQQRLGLAYVFIA
                     HDLAVVKHIAARVAVMYLGRIVEYASTRELFARPRHPYTQALLSAIPVPVPGARAQ"
     gene            complement(819..>1634)
                     /locus_tag="VHT22_04520"
     CDS             complement(819..>1634)
                     /locus_tag="VHT22_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018067026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3136848.1"
                     /translation="KGGAEVSGRVNFEGRDLLREPDRVMRDLRGNRLAMIFQEPMTSL
                     NPAFTIGDQIVEGIIRHRPASEATARALALEMLRRVHIASPEQRFDDYPHRLSGGMRQ
                     RVMIAMALACKPALLIADEPTTALDVTVQAQILDLMRQLRRETGTAIILITHDLGVVA
                     ELADDVVVMYAGRIVESASVAALFAEPQHPYTVGLLGSIPRLDLEQTRLAAIEGQVPN
                     PMAPVDGCRFHPRCPFAFERCRREDPPLLDMGGGHRSACWRAPLDPDALVPPA"
BASE COUNT          276 a          549 c          572 g          237 t
ORIGIN      
        1 ttgcgcccgt gcgccgggga cgggcaccgg aatcgccgaa agcagcgcct gcgtgtacgg
       61 atggcgcggc cgcgcgaaca actcgcgtgt ggacgcgtac tcgacgatgc ggccgagata
      121 catgacggcc acgcgcgcgg cgatgtgctt gaccaccgcg agatcgtgcg cgatgaacac
      181 ataggcgagc ccgagccgct gctgcaggtc catcagcagg ttgatcacct gtgcctgaat
      241 cgatacgtcg agcgccgaca ccggttcgtc gcagacgatc agccgcggct cgacggccag
      301 cgcccgtgcg atgccgatcc gctgccgctg tccgccggaa aattcatgtg gatagcgttc
      361 ggtgtgctgc ggcgccagac cgaccaggcc gagcagctca gcgactcgat cgctgcgccg
      421 gccggacgcg agaccgtaca gcgccagcgg ctcgccgacg gtctgggcga cggtcatgcg
      481 cggattcagc gacgcgtacg ggtcctggaa gatgatctgc agcgcgcgcc gttgtgcgcg
      541 catcgcctcg gcgtcgagtg cgaagagctc ttgaccgtcg aagcgcacgc tgccggccgt
      601 cggctcgata aggcgcagca agagccgtcc ggtcgtcgac ttgccgcagc cggattcgcc
      661 gaccagtgcc agcgtctcgc cggccgcaag atcgaaatcg attccgtcga cggcgcgcac
      721 aacccgcgca tcgcggcgga agaaaccttt gcgcagcggg aaatgcttga ccagcccggc
      781 gacctgcaac agcggcgtat ccagggcagg gaccgcggtc atgccggcgg caccagagca
      841 tcggggtcga gcggtgcgcg ccagcacgcc gaccggtgac caccgcccat atccagcagc
      901 ggcggatcct cgcgacggca ccgctcgaac gcaaacgggc agcgcggatg gaagcggcag
      961 ccgtcgaccg gcgccatcgg attcggcacc tgtccctcga tggccgccag ccgggtctgc
     1021 tcgagatcca gccgcgggat cgagcccagc agtccgaccg tatacggatg ctgcggctcg
     1081 gcgaataagg ccgcgacgct cgcactttca acgatgcgtc cggcgtacat gacgacgacg
     1141 tcgtcggcga gttcggcgac cacgcccaga tcgtgcgtga tcaggatgat cgccgtgccg
     1201 gtttcccggc ggagctggcg catcaggtcg aggatctgcg cctggacggt cacgtcgagc
     1261 gccgtcgttg gctcgtccgc gatcaggagc gccggcttgc aggcgagtgc catcgcgatc
     1321 atcacgcgct ggcgcatgcc gccggagagc cggtgcgggt agtcgtcgaa gcgctgttcg
     1381 ggggacgcga tatgcacgcg gcgcagcatc tccagcgcca gcgcccgggc agtcgcctcg
     1441 ctggccgggc gatggcggat gatgccctcg acgatctgat cgccgatggt gaacgccgga
     1501 ttgagcgagg tcatcggttc ctggaagatc atggcgagcc gattgccgcg caggtcgcgc
     1561 atgacccggt cgggctcgcg caggagatcg cggccctcga agttgacccg gcccgaaacc
     1621 tcggcgccgc cctt
//
LOCUS       DATANS010000314         1688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_15564, whole genome shotgun sequence.
ACCESSION   DATANS010000314 DATANS010000000
VERSION     DATANS010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15564"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VHT22_04525"
     CDS             <1..293
                     /locus_tag="VHT22_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HEX3136849.1"
                     /translation="LECAHKGWGKSTIIGVAGAGQEISTRPFQLVPGRVWQGSAFGGA
                     RGRTDVPRIVDWYMEGKINIDDLITHTLPLERINEGFDLMHAGESIRAVVRY"
     gene            293..1681
                     /locus_tag="VHT22_04530"
     CDS             293..1681
                     /locus_tag="VHT22_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX3136850.1"
                     /translation="MTTNFALWALVAGVLLIAMAFLGTWVERMPLSPAMLYLGAGFAL
                     GPSGLGMLLPDPKAYSPLLERLAEIAVVISVFVTALRISVPLKDIRWRLPISLATVAM
                     VATIALVAVVGVAWLALPLGAAILLGAILAPTDPVLASDVQLDDPRDRDLLRFSLSGE
                     AGLNDGAALPLVLLGMGMLGLHELGGAGGQWLLVDVLWGVGGGLGIGWLLGAWVSTLI
                     LHLRTRHGHAIGLNEFLALGLIALVYGIALLAHASGFLAVFAAGLALPRMRPMHETDS
                     RPRLPVAMTPGAAAEIEIGEASAPANATHPVKAPAHMAQAVLSFDEQLERFAEIAMVL
                     AVGAMFSYAVIPSSALWFVPLLLLVIRPVSILIAGAPAMSSRAQQPLIAWFGIRGIGS
                     VYYLLYAVNRGLPDAVAPTVIGLTLVTVVTSIVVHGVSIPPLMNRYRHRKGGARAGTG
                     QDGRHRRRKRAA"
BASE COUNT          241 a          537 c          591 g          319 t
ORIGIN      
        1 cgctcgaatg cgcgcacaag ggctggggca agagcaccat catcggcgtc gccggggcgg
       61 ggcaggagat cagcacacgg ccgttccagc tggtccccgg ccgggtctgg caagggagcg
      121 ccttcggcgg cgcgcgcgga cggacggacg tgccgcggat cgtcgactgg tacatggaag
      181 gcaagatcaa catcgacgac ctgatcaccc acacgctgcc gctcgagcgg atcaacgagg
      241 gattcgacct gatgcatgcc ggcgaatcga ttcgcgccgt cgtgcggtat tgatgacaac
      301 caattttgcg ctgtgggcgc ttgtcgcagg cgtgctgctg atcgcgatgg catttctcgg
      361 cacctgggtc gagcgcatgc cgctcagtcc ggcgatgctc tacctcggcg caggcttcgc
      421 gcttgggccc agcggcctgg gcatgctgct gcccgatccg aaagcctatt cgcctctgct
      481 cgaacggctg gcggaaatcg cggtcgtgat ttccgttttc gtcaccgcgc tgcgcatcag
      541 cgtgccgctc aaggacatcc gatggcgatt gccgatcagc cttgcgaccg tcgcgatggt
      601 cgcgacgatc gcgctggttg ccgtggtcgg cgtcgcgtgg ctggcgctgc cgctgggcgc
      661 tgcgatattg ctgggtgcga tcctggcgcc caccgacccg gtgctcgcct cggacgtgca
      721 actggacgat ccgcgcgatc gcgatctgtt gcgcttcagc ttgagcggcg aggcggggct
      781 gaacgacggc gcagcgctgc cgcttgtcct gttgggcatg ggcatgctcg gcctgcacga
      841 gcttggcggc gcgggcgggc aatggctgct ggtcgatgtg ttatggggtg ttggcggcgg
      901 gctggggatc ggctggctgc tcggcgcgtg ggtcagcacg ctgatcctgc acttgcgcac
      961 gcgccatgga cacgcgatcg ggctcaacga atttctcgca ctgggcctga tcgccctggt
     1021 ctatggcatc gcgttactgg cgcatgcgtc cggattcctt gcggtctttg ccgccggcct
     1081 cgcgctgccg cggatgagac cgatgcacga aaccgattcg cgcccgcgct tgccggtcgc
     1141 gatgacgccg ggcgccgcgg ccgagatcga aataggcgag gcgtccgcgc cggcgaacgc
     1201 gacgcacccg gtcaaggctc cggcacacat ggcgcaggcc gtgctgagct tcgacgagca
     1261 gctggagcgc ttcgccgaaa tcgcgatggt gctggccgtc ggcgcgatgt tctcgtacgc
     1321 ggtaattcct tcgagcgcgc tttggttcgt gccgctgctg ctcctggtga tccggccggt
     1381 cagcatactg atcgccggcg cgccggcgat gtcttcacgc gctcagcagc cgctgatcgc
     1441 ttggttcggc atacgcggca tcggttcggt ctattatttg ctgtatgcgg taaaccgcgg
     1501 cctgccggac gcggtggcgc cgacggtcat cggcctgacc ctggtcaccg tcgtgacgtc
     1561 gatcgtcgtg cacggcgtct cgattccgcc gctgatgaat cgctatcgtc accgcaaggg
     1621 cggcgcgcgt gcagggaccg gacaagatgg ccgacatcgt cggcgcaaac gcgccgcgta
     1681 ataaacgc
//
LOCUS       DATANS010000315         3150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_647234, whole genome shotgun sequence.
ACCESSION   DATANS010000315 DATANS010000000
VERSION     DATANS010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647234"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VHT22_04535"
     CDS             <1..241
                     /locus_tag="VHT22_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX3136851.1"
                     /translation="RRWSIYDAVVIHRVGELAPTDQIVLVAVSSAHRGDAFAACEFII
                     DYLKTQAPFWKKELTPDGERWVEARASDDEAAARW"
     gene            396..1307
                     /locus_tag="VHT22_04540"
     CDS             396..1307
                     /locus_tag="VHT22_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014722422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEX3136852.1"
                     /translation="MQDIALLAIDWGTSSARVYAMDRQGRIRDTRSAPLGVQRVASGE
                     FAQALKTLRGDGFPESVPLIACGMIGSRQGWVEASYCACPADFDTIAAALTRVPGTAL
                     AIVPGLICRDDDGVADVMRGEETQILGALADDARTRQVMLLPGTHSKWAIVGPAGIEA
                     FSTFMTGELYALLVEHSILGRLAAPGSDEEAFARGVRHSLRGTGALTHDLFSARTLAL
                     TNRLAPSAVGDYLSGLLLGTEIAAAKRWLERMGAAATRLTLVGARALVDKYRAALTIA
                     GIDADIGAEDAAARGLWRIARHAGLLR"
     gene            1304..1942
                     /locus_tag="VHT22_04545"
     CDS             1304..1942
                     /locus_tag="VHT22_04545"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HEX3136853.1"
                     /translation="MTTALSDYLSPLPLVAVLRGITPSEIAAVGSALTEHGFRILEVP
                     LNSPEPFDSIKRLAEIYGERCLTGAGTVIKVADVARVADAGGRLIVMPHGDVAIVREA
                     KRRGLVCLPGVATPTEAFAALDAGADGLKMFPAEQLSPAVLKAWRAVLPRGTLVFPVG
                     GIRPDNMAPFWAAGADGFGTGSNLYKPGASAETVRASASAYASGFRALQRER"
     gene            1939..2874
                     /locus_tag="VHT22_04550"
     CDS             1939..2874
                     /locus_tag="VHT22_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX3136854.1"
                     /translation="MSGPRFDLVALGEPLLEFNQTREASDQYLQGFGGDTSNCVIAAA
                     RLGAATSYITRLGDDAFGRKFLALWQRERVDASGVAIDAEASTGIYFVTHGASGHEFS
                     YLRAGSAASRMRPSDLPLPVIRSAKVLHVSGISQAISASACDTVFAAIDAAREAGARI
                     SFDCNVRPKLWPLPRARAVIVATMRLCDWFLPSLDEAVQLSGKEQPDAILDWCHLQGA
                     PVVALKMGGEGVWVSTGEARERIAGMRVNAIDATGAGDCFDGAFAARLVAGDTPFAAG
                     RYANAAAALATTGYGAVAPLPRHADVAALLATAVR"
     gene            2871..>3150
                     /locus_tag="VHT22_04555"
     CDS             2871..>3150
                     /locus_tag="VHT22_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3136855.1"
                     /translation="MTGETFRSGLFAEQVALVSGGTSGIGAAIAALLTRLGARVTVTG
                     ASQAEADAARAAAGFAAERAIGLDVRDGAAIAACLRDFPRLDMLINCAG"
BASE COUNT          474 a         1015 c         1104 g          557 t
ORIGIN      
        1 gaggcgctgg tcaatctatg acgcggtggt catccatcgg gtcggcgagc tcgcgccgac
       61 cgaccagatt gtcctggtcg cggtgtcgag cgcgcatcgc ggcgatgcct tcgccgcctg
      121 cgaattcatc atcgactacc tgaagaccca ggcgccgttc tggaaaaagg aattgacgcc
      181 cgacggcgag cgctgggtcg aggcgcgcgc cagcgacgac gaagcggcag ctcgctggta
      241 gatcgcgcga gatccgcgcg taggcgcagc gtgctgccat ttgcgccggc ttgaccctga
      301 agcggtttcg gttctagagt gcgactcgct gcgcatacgg cggccaatcg ccgcggtcaa
      361 aaacatccgc cacgcagcgc tcgaatcttc ccgccatgca ggacatcgcc ttgcttgcca
      421 tcgattgggg tacgagctcg gcccgcgtct atgcgatgga tcgtcagggc cgtatccgtg
      481 atacgcgcag tgcaccgctg ggcgtgcaac gggtcgccag cggtgaattc gcgcaagcgc
      541 tcaagacgct gcgtggcgac ggctttcccg aaagtgtgcc tttgatcgct tgcgggatga
      601 tcggcagccg ccagggctgg gtcgaggcgt cgtattgcgc ttgtcccgcc gacttcgaca
      661 ccatagcggc agcgctgact cgcgtccccg gcacggcgct ggcgatcgtt ccaggtttga
      721 tatgtcgaga tgacgatggc gtcgccgacg tcatgcgcgg cgaggaaacg cagattctcg
      781 gcgcgctggc cgacgatgcc aggacgcggc aagtcatgct tctacccggc acccacagca
      841 agtgggcaat tgtcggcccc gccgggatcg aggctttttc gacattcatg accggcgagc
      901 tctacgcact gctggtcgaa cacagcattc tcggaaggct ggccgcgccc ggcagcgacg
      961 aagaggcctt tgcgcgcggc gtgcgtcaca gccttcgcgg caccggggcg ctcacccacg
     1021 atttgttcag cgcgcgtacc ttggcgctga ccaatcgact tgcgccttcg gccgtcggcg
     1081 attacctttc gggcttgttg ctcggcaccg agatcgcggc ggcgaagcgc tggctcgagc
     1141 ggatgggtgc tgctgctaca cgcctgactc tggtcggggc acgagctctg gtcgataagt
     1201 atcgagcggc attgacgatc gcggggatcg atgcagacat cggtgcggaa gacgccgcgg
     1261 cgcgcgggtt gtggcggatt gcgcggcacg cgggactgtt gagatgacga ctgctctctc
     1321 cgattatctg agcccgctgc ctctggtcgc cgtcctgcgc ggaatcacgc cttccgagat
     1381 cgcagcggtc gggtcggctc tcaccgagca cggctttcgc atcctcgagg taccgctgaa
     1441 ttcgcccgag ccgttcgaca gcatcaagcg gttggcggag atctacggcg agcgctgttt
     1501 gaccggcgcg ggtaccgtga tcaaggtcgc tgatgtcgcg cgggtcgccg atgcgggcgg
     1561 gcggctaatc gtcatgccgc atggcgatgt cgcgatcgtg cgcgaggcca agcggcgcgg
     1621 cttggtctgt cttcccggcg tggccacgcc gaccgaagcc ttcgccgcgc tcgacgccgg
     1681 cgccgacggg ctcaagatgt ttccggccga gcagctgtcg ccggcggtgc ttaaggcatg
     1741 gcgcgccgtg ctgcctcgcg gaacactggt gtttcccgtc ggcggcatcc gcccggacaa
     1801 catggcgccg ttctgggccg ccggcgccga cggattcggc acgggctcga atctgtacaa
     1861 gccgggtgcc tcggccgaaa cggtgcgcgc ctcggcctcc gcctatgcgt ccggctttcg
     1921 ggcattgcag cgcgagcgat gagcggaccg cgcttcgacc tggtggctct gggcgaaccg
     1981 ctgctcgagt tcaaccagac gcgcgaagcg agcgatcagt acctgcaggg attcggcggc
     2041 gacacgtcga actgcgtcat tgccgcggcg cggctcggcg ccgcgacctc ctacatcacg
     2101 cgcttgggcg acgatgcgtt cggccgcaag ttcctcgcac tgtggcagcg cgagcgcgtc
     2161 gatgccagtg gtgtggcgat cgatgccgaa gccagcactg gcatctactt tgtcacgcac
     2221 ggcgcgagcg gccacgagtt cagctatctt cgcgccggct cggcggcgag ccggatgcgt
     2281 ccgtcggatc tgccgctgcc ggtcattcga tcggccaagg tgctgcacgt ctcgggcatc
     2341 agccaggcga tcagcgccag cgcctgcgac acggtgttcg cggcgatcga cgcggcgcgc
     2401 gaggccggtg cgcggatctc cttcgattgc aatgttcgcc cgaagctctg gccgctgccg
     2461 cgcgcacggg cggtaatcgt ggcgacgatg aggctgtgcg actggttcct gccgagtctg
     2521 gacgaagccg ttcagctgtc cggaaaggag cagccggatg cgattctcga ttggtgccat
     2581 ctgcagggcg cgccggtcgt cgcgctgaag atgggcgggg aaggcgtctg ggtctccacc
     2641 ggagaggcgc gcgagcgcat cgccggcatg cgtgtcaacg caatcgatgc gactggagca
     2701 ggcgactgtt tcgacggcgc gtttgccgca agactcgtcg ccggggacac gccgtttgcg
     2761 gcgggacgtt atgccaatgc agcggcggcg ctggcgacga ccggatatgg cgcagtcgcg
     2821 ccgctgcctc ggcacgccga cgtggctgca ttgctggcta ccgcagtccg atgacaggcg
     2881 agaccttccg cagcgggctc ttcgcggagc aggtagcgct ggtctcgggg gggaccagcg
     2941 gcatcggcgc tgcaatcgct gccttgctga cccgcctcgg cgcgagggtc accgtcaccg
     3001 gggcgtcgca ggcggaggct gatgcggcac gcgccgccgc tggcttcgcc gccgaacgcg
     3061 cgatcgggct cgacgtgcgc gatggagccg ccatcgcagc atgcctgcgc gattttcccc
     3121 gcctcgacat gttgatcaac tgcgcaggcg
//
LOCUS       DATANS010000316         2032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_58427, whole genome shotgun sequence.
ACCESSION   DATANS010000316 DATANS010000000
VERSION     DATANS010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58427"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1228)
                     /locus_tag="VHT22_04560"
     CDS             complement(83..1228)
                     /locus_tag="VHT22_04560"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEX3136856.1"
                     /translation="MPAKWNPELEFKPSRELSIGVELELQILNSRDYNLARDAGDLLA
                     LLERTPHPGAVKPEITESMIEVNTSVHVKFATLRAELLEIRNAVSKAGAQLNLRIAGG
                     GSHPFHKWSDRRIYPTERFKHLLSVYGYLAKQFTVFGQHIHVGVPDGDTAIHLTHALS
                     RYIPHFIALSASSPFQQGTDTDFQSSRLNTVSAFPLSGTLPLVHTWSEFVAYFEKMRR
                     FGIVESMKDFYWDTRPKPEFGTVEIRVLDTPLTVERAAQLAAYAQTLAHYLLTQQPLP
                     TSDDVYLVYKYNRFQACRYGVGGAYIDAQTQQRGTLQRDILDTLDLLRPHARTLETEA
                     PLSALRSEAQASKSDAAWLREAYQKLGSLNDVARRQSEQWMGSPLNA"
     gene            complement(1229..>2032)
                     /locus_tag="VHT22_04565"
     CDS             complement(1229..>2032)
                     /locus_tag="VHT22_04565"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX3136857.1"
                     /translation="IGVSSSPAIVLLVARELRAEGQVTERALNLVAINSVVAFVLTTM
                     LLSWIHREYQAGWFVILLHPLYILAGSCFLGLAASWLALKLAGWLGKRIELHAALLLG
                     IVVLLVGAAEALKLSILLSLLTFGVLIGNMDREHKLMPVDVGRFSQIFFVVLFVASGA
                     LLHIGDLVAGGAVAAVYIAARFVGKSIGVLCFAHLSGIRAGSGGLLSIALLPMSGIAL
                     AIAQSTGTSYPGFSAKLSVIVLAATLILELIGPLAVQFALRRAGEAQEE"
BASE COUNT          392 a          652 c          652 g          336 t
ORIGIN      
        1 gatgaagcga atcgagacgc tgatcacgca ctaacccggg gggcgcggca cccggccggc
       61 gcggcacccg gccggcgcgg gctcacgcgt tgagcgggct gcccatccac tgctcgcttt
      121 gccggcgcgc gacatcgttc agagacccga gtttctgata ggcctcgcgc agccaggcgg
      181 cgtcgctctt ggaggcctgg gcctcggagc gcagcgcgct taacggcgct tccgtttcga
      241 gcgtgcgcgc gtgaggccgc aataaatcaa gcgtatccag aatgtcgcgt tgcagcgtgc
      301 cccgttgctg cgtctgcgca tcgatgtacg caccccccac gccgtagcgg caggcctgga
      361 accggttgta tttatacacg agatagacgt cgtccgacgt cggcaacggc tgctgcgtga
      421 gtaggtaatg cgcgagcgtt tgcgcatagg ccgcgagctg cgcggcgcgc tcgaccgtca
      481 gcggcgtgtc gagcacgcga atctcgacgg tgccgaattc cggcttgggc cgcgtgtccc
      541 agtagaaatc tttcatgctc tcgacgatgc cgaagcgccg catcttctcg aagtaagcga
      601 caaactcgct ccatgtatgc accagaggca gggtgccgga cagcgggaat gcgcttacgg
      661 tgttcaggcg agaagattga aaatccgtgt ccgtgccctg ctgaaacggc gacgaggccg
      721 acagcgcgat gaaatgcggg atataacggg acaacgcgtg cgtgaggtgg atcgccgtgt
      781 cgccgtcggg cactccgacg tggatgtgct ggccgaatac ggtgaattgc ttcgcgagat
      841 agccgtacac tgacagcaga tgcttgaacc gctcggtcgg gtagatgcgc cgatcgctcc
      901 atttgtgaaa cgggtgcgac ccgccgccgg cgatgcgcag gttcagctgc gcgccagctt
      961 tgctgaccgc attgcggatt tccagcagtt ccgcgcgcag ggttgcaaat ttcacgtgca
     1021 ccgacgtatt gacctcgatc atgctctcgg taatttcggg tttgaccgca cccggatgcg
     1081 gcgtgcgctc gagcagcgcc aacaggtcgc ccgcgtcgcg cgcgaggttg taatcgcgcg
     1141 agttcagtat ctgcagctcg agctcgacgc cgatcgacag ctcgcgcgac ggcttgaact
     1201 cgagctcggg attccacttg gcaggcactt attcctcctg cgcctcgccc gcgcggcgca
     1261 gcgcgaactg cacggcgagc gggccgatca attcgaggat caacgtggcc gccagcacga
     1321 tcacgctcaa tttcgcgctg aatcccggat acgaggtgcc ggtactctgt gcgatggcga
     1381 gcgctatgcc cgacatcggc agcagcgcga tgctgagcag gccgccgctg ccggcgcgga
     1441 tgccgcttaa gtgcgcaaag cacagcacgc cgatcgactt gccgacgaac cgcgcggcaa
     1501 tgtagaccgc ggcgaccgcg ccgcccgcga caaggtctcc gatgtgcagc agcgcgccgc
     1561 tcgcaacgaa cagcacgacg aagaaaatct ggctgaaccg gcccacgtcc accggcatca
     1621 gcttgtgctc gcggtccatg ttcccgatga gcacgccgaa cgtgagcagc gacagcagga
     1681 tcgacagctt gagagcctcg gccgcgccca ccaacaggac gacgatcccg agcagcaacg
     1741 ccgcatgcag ctcgatacgc ttgcccagcc agccggccag cttcagcgcg agccagctcg
     1801 cggcgagccc gagaaaacac gagcccgcga ggatgtaaag cggatgcagc aatattacga
     1861 accagcccgc ctgatattcg cggtgtatcc acgacaaaag catcgtggtc agcacgaagg
     1921 cgacgacgct gttgatcgcg accagattca gcgcgcgctc ggtcacctgg ccttcggctc
     1981 tcaattcgcg cgccaccagc aggacgatgg ccggggacga cgagacgccg at
//
LOCUS       DATANS010000317         3221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_771369, whole genome shotgun sequence.
ACCESSION   DATANS010000317 DATANS010000000
VERSION     DATANS010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771369"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VHT22_04570"
     CDS             complement(<1..601)
                     /locus_tag="VHT22_04570"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX3136858.1"
                     /translation="MQIPKTVWVLAAIAAIVAITLAFFDWNWLRGPLASYLSAQVGRP
                     VKIEGNFRVALSTRPLLTADYLTVGNAPWSTEPTMASAQRVNVRLDLRSLLFGPLVLH
                     EASMVRPRLLLEKSADGKENWEIGGAGPPALPLPGRLKIEDGVIHYRDPGAGTDVSVN
                     IASSASSVSGATPVHFSGAGRLRNSAFTIEGDAESLLALE"
     gene            752..2296
                     /locus_tag="VHT22_04575"
     CDS             752..2296
                     /locus_tag="VHT22_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX3136859.1"
                     /translation="MLGPSAASAAPLNVGSKRFTESYILGEILAQTAQGAGEATVVHR
                     QGLGNTAIVLGALTQGSIDVYPEYTGTIAKEILKLDSVPPLPELNAKLAAMGLGVAVP
                     LGFNNTYALAMRADDARAKGITRLSDLKSHPELRLGLSQEFIGRADGWPGLKRAYELP
                     YETPRGLDHGLAYEAIAQRQVDAIDIYSTDAKIDKYGLTVLSDDRNYFPRYDAVLLYR
                     ADLPQRLPRTFAAFRRLEGRIDDASMRRMNAEAELEGKSFVTVARDFLAQKSAGGAPP
                     ASAVASSAKAPASDAQTESGSGFWQKLFGPDFARLTLEHLLLVFASLAASIALGIPLG
                     ILAARRPASEGVILGMTGVVQTIPALALLAFLIPITGRIGAIPAFLALALYALLPIVR
                     NTHAAMTQIGRGVKQAAQSLGLENGTILRYIELPLGAPTILAGIKTSAVINVGTATIA
                     AFIGAGGYGERIVTGLALNDHAMLLAGAIPAAALALLIEGAFRLGERWLVPAGMRLSE
                     QVARNG"
     gene            complement(2318..2677)
                     /locus_tag="VHT22_04580"
     CDS             complement(2318..2677)
                     /locus_tag="VHT22_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004525578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HEX3136860.1"
                     /translation="MRTRRYRLALALICAGALLPFAVVFAAGPADAASAAKPATHRVV
                     IDGLKYEPETLTVRRGDTIVWTNKDPFPHTVTAPGKFDSHDIAANGSWKYVARTAGDY
                     AYICTLHPNMKGTLRIE"
     gene            complement(2674..3186)
                     /locus_tag="VHT22_04585"
     CDS             complement(2674..3186)
                     /locus_tag="VHT22_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEX3136861.1"
                     /translation="MKNIGIVVASALFLAAGSVAAQAPNDAQIAHIVVTANQVDIDAG
                     KLAEKMGSTKAVKDFGKQMVTDHSGVNKQAVALVTKLKVTPEDNPTSQSLKSGGQENV
                     KNLKTLKGKAFDKAYIDHEVAYHQQVLDAVDKTLLPSAKNEELKALITKVRPAFVAHL
                     EHAKMLQSKM"
BASE COUNT          532 a         1057 c         1031 g          601 t
ORIGIN      
        1 cctcgagcgc gagcaaggat tcggcgtcgc cctcgatcgt gaaggcgctg ttgcgcaggc
       61 gcccggcgcc cgaaaaatgc accggtgtcg ctccgctcac cgaagatgcc gacgacgcga
      121 tgttgacgct gacgtcggtc cccgcacccg gatcgcggta gtggataacg ccatcctcga
      181 tcttcagacg gccgggcaag ggcagtgccg gcggccccgc gccgccgatt tcccagttct
      241 cctttccgtc cgcgcttttt tcgagcagca gccgcgggcg caccatgctg gcttcatgca
      301 gtacgagggg cccgaacaat agcgagcgaa ggtccaagcg gacgttgacg cgctgtgcgc
      361 tcgccatggt cggctcagtg ctccacggcg cgttgccaac cgtcagatag tcggcggtca
      421 gcagcggcct ggtcgacagc gcgacgcgaa aattcccctc gatcttgacc ggccgtccga
      481 cttgcgcgga aaggtagctc gccaatgggc cgcgcagcca gttccagtcg aagaaagcca
      541 gcgtgattgc gacgatggcc gcaatcgcgg caagaaccca gactgttttt ggtatctgca
      601 tgatgctgcc ggctcacccc ctagggcttt gaattcgcgg cgccatgata gtttcgtctg
      661 ccggccctgt gtcggtcatg cgcctacaat atgcgccatg aatgccccgt gcctcgctgc
      721 gcgaatcgga ttcgccctcg ctattgtcgt tatgctcggc cccagcgcgg catcggcggc
      781 gccgctcaac gtcggctcca agcgcttcac cgaatcctac atcctcggtg agattctcgc
      841 gcagacggcg caaggcgccg gcgaagcgac ggtcgtgcac cgccaggggc tgggcaacac
      901 ggcgatcgtg ctcggtgccc tgacgcaggg cagcatcgat gtctatcccg aatacaccgg
      961 cacgatagcc aaggagatac tgaagctcga ctccgtgccg ccgctgccgg agctcaacgc
     1021 caagctcgcg gcgatgggcc tcggcgtcgc ggtgccgctg gggttcaaca acacttacgc
     1081 actggccatg cgcgccgacg acgcgcgcgc caagggaatc acgcggctct ccgatctcaa
     1141 gtcgcatccg gagctccggc tcgggctgtc gcaggaattc atcgggcggg ccgacggctg
     1201 gcccggattg aagcgcgcct acgagcttcc ctacgagacg ccgcgcgggc tcgatcacgg
     1261 tctcgcctac gaggcgatcg cccagcgcca ggtcgatgcg atcgatatct attcgaccga
     1321 cgccaagatc gacaagtacg gtctgacggt gctcagcgac gatcgcaact atttcccgcg
     1381 ctacgatgcc gtgctgctct accgcgccga cttgccgcag cgcctgccgc gcaccttcgc
     1441 cgcattccgg cgcctcgaag gccggatcga cgatgcctcg atgcggcgga tgaatgccga
     1501 agccgagctc gagggcaaaa gcttcgtcac cgtggcgcgc gacttcctcg cgcaaaaatc
     1561 tgccggcggc gcgccgcctg cgagtgcggt cgcctcatcc gcgaaggcgc cggcgagcga
     1621 tgcgcagacg gagtcgggca gcggcttctg gcaaaagctg ttcgggccgg atttcgcgcg
     1681 actgacactc gagcatttgc tgctggtgtt cgcctcgctt gcggcaagca ttgcgctggg
     1741 catcccgctg ggtatcctcg ccgcgcggcg gcccgccagc gaaggcgtga tcctgggcat
     1801 gacaggcgtg gtgcaaacga tccccgcgct ggcgctcctg gccttcctga tcccgatcac
     1861 cgggcgcatc ggcgcgattc cagcattcct cgcactcgcg ctgtatgcgc tgcttccgat
     1921 cgtgcggaac acgcacgcgg cgatgaccca gattggacga ggggtcaagc aggcggcgca
     1981 gtcgctgggc ctggaaaacg gaacgatcct gcgttacatc gagctgccgc tcggcgcgcc
     2041 gacgatactc gccggtatca agacttcagc ggtgatcaac gtcgggacag cgacgatcgc
     2101 cgcattcatc ggcgccggcg gctatggcga acgcatcgtc accgggctcg ccctcaatga
     2161 ccacgcgatg ctgctcgccg gtgcgattcc ggcggcggcg ctggcgcttc tgatcgaagg
     2221 agctttccgc ctcggcgagc gttggctcgt gccggccgga atgcgcctga gcgagcaggt
     2281 cgcgcgcaat ggctgaaaag agcccaaagg ccgcggccta ttcgatccga agcgtgcctt
     2341 tcatgtttgg gtgcagcgtg cagatgtagg cgtaatcgcc ggcggtgcgc gcgacgtact
     2401 tccacgagcc gttcgccgcg atgtcgtgcg aatcgaactt gcccggcgcc gtcaccgtgt
     2461 gcggaaacgg atccttgttg gtccacacga tcgtgtcgcc gcgcctgacc gtcagcgtct
     2521 cgggctcgta cttgagaccg tcgatgacga cgcgatgcgt cgccggcttg gcagcgctcg
     2581 cggcgtcagc cggaccggcg gcgaaaacga cagcgaatgg cagcagcgcg cccgcgcaaa
     2641 tcaacgcgag cgcgagccga tacctgcggg tcctcacatc ttggactgca gcatcttggc
     2701 gtgctccaga tgcgcaacaa acgcggggcg gaccttggtg atcagcgcct tgagctcctc
     2761 gttcttggcg ctcggcagca gcgtcttgtc gacagcgtcc agaacctgct ggtggtaggc
     2821 gacttcgtgg tcgatgtagg ccttgtcgaa cgccttgccc ttgagcgtct tcaggttctt
     2881 cacgttctct tggccgccgg actttaagct ctggctggtc gggttgtctt ccggggtgac
     2941 tttgagcttg gtgaccagcg cgaccgcctg cttgttgacg ccgctatggt cggtgaccat
     3001 ctgcttgccg aagtccttga cggccttggt gcttcccatc ttttcggcca gcttgcccgc
     3061 gtcgatgtcg acctggttcg cggtgacgac aatatgcgcg atctgcgcgt cgttcggagc
     3121 ctgggcagcc acgctgcctg cggcgaggaa tagagcgctt gcaacgacta taccgatgtt
     3181 tttcatgatg atctcctgag aatgaaacat tgccctgctt c
//
LOCUS       DATANS010000318         2568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_142850, whole genome shotgun sequence.
ACCESSION   DATANS010000318 DATANS010000000
VERSION     DATANS010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142850"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VHT22_04590"
     CDS             complement(<1..549)
                     /locus_tag="VHT22_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HEX3136862.1"
                     /translation="MPLSSETREHLKRASAATLTTQLFKRGLRNVFIQGVHRMSAASD
                     NLVGEAFTLRYIPAREDLDQLSVFENPEHPQRKAIETVPPGHVLMIDCRRDTRAASGG
                     HILLTRLKVRGAAGLVTDGCLRDSEAIAAMDFPVYGAGGSAPLNLVCHHAVDINVPIG
                     CGGVAVFPGDAIVGDGEGVVVIP"
     gene            complement(630..1985)
                     /locus_tag="VHT22_04595"
     CDS             complement(630..1985)
                     /locus_tag="VHT22_04595"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEX3136863.1"
                     /translation="MSRRIEFPADFLWGGATSAYQVEGSPLADGAGPSIWQRFCHTPN
                     MVRDGDTGDVACDHYRRYREDVALMREIGLNAYRFSISWSRVLPSGRGPVNAAGLDFY
                     ERLVDTLLQQGIEPMVTLFHWDLPAALDDLGGWLNPDIAGWFADYASAAFRRLDGRVK
                     LWATLNEPWVVTDGGYLHGALAPGHRNRFEAPIAAHQLMRAHGGAVKAYRSEGKHRIG
                     VVVNLEPKYAASEDAADRAATARADAYMNRQFLDPMLLGRYPDELAEIFGEAWPSWPA
                     GDLELINQPLDFVGVNYYTRNVTRFDPDAWPLRAAPVRQKQATYTETGWEVFPQGMTD
                     ILVWVKHRYGNPPLYVTENGAAFFDPPTAQADRVDDPLRVDYLRKHIAAIHAAREAGV
                     DLRGYFAWSLLDNFEWSLGYSKRFGIVHVDFETQKRTPKNSARLYSSIIASNGAVLNE
                     P"
     gene            complement(2011..>2568)
                     /locus_tag="VHT22_04600"
     CDS             complement(2011..>2568)
                     /locus_tag="VHT22_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEX3136864.1"
                     /translation="KTAWDVYTPYEMRIVGTLVRLGWRDRAQALLAFFLADRRPLAWN
                     QWPEVVGRDPRQPRFVGDMPHAWVASDFIRATLDLFAYERDADHAVVLAAGIAPDWLD
                     QGGVALQNLRTPYGRLSYTLRRDGDRVTLRLAAGSGMPPGGFIFVWPGDKPPRSATIN
                     GKPATFSGAELHVGAVPARVLIDGR"
BASE COUNT          446 a          840 c          842 g          440 t
ORIGIN      
        1 cggaatgacg acaacgcctt cgccatcgcc gacgatcgcg tcacctggga acactgcgac
       61 accgccgcat ccgatcggca cgttgatatc gacggcgtga tgacatacca gattcaacgg
      121 cgcgctgcct cctgcgccgt agacgggaaa gtccatcgcc gcgatcgctt cgctgtcgcg
      181 caggcagccg tctgtgacca gtccggcggc gccgcgcacc ttgaggcgcg tcaggaggat
      241 atgacctccc gatgcggcgc gggtgtcgcg ccggcaatcg atcatcagca cgtggccggg
      301 aggaaccgtt tcgatcgcct tgcgctgcgg gtgctcggga ttctcgaaga cgctcagttg
      361 gtcgagatcc tcgcgcgccg ggatatagcg cagcgtaaag gcttctccga ccagattgtc
      421 cgaagccgcg ctcatccggt gcacgccctg gatgaacacg ttgcgcagtc cccgcttgaa
      481 caattgtgtc gtcagcgtgg cggcgctcgc gcgcttgagg tgctcccggg tttcactgga
      541 cagtggcatc gaagcgtcgc tttcggaaat gatgttcgcg gcgagggcgc tatgatagcg
      601 cggacgctga tcggccccac ggccgcggct cacggctcgt tcaagaccgc cccgttgctc
      661 gcgatgatgc tcgaatagag gcgcgcgctg ttcttgggcg tgcgcttctg ggtctcgaag
      721 tcgacgtgca ctatgccgaa acgcttcgag tagccgagcg accattcgaa attgtccagc
      781 agcgaccagg cgaaatatcc gcgcaggtcc actcccgcct ccctcgccgc gtgaatcgct
      841 gcgatgtgct tgcgcaggta atcgactcgc aacgggtcgt cgacgcgatc ggcttgcgcc
      901 gtcggcggat cgaaaaacgc ggcgccattc tcggtcacgt acagcggagg attgccgtaa
      961 cggtgcttga cccagaccag gatgtcggtc atgccttgcg ggaacacctc ccagccggtt
     1021 tcggtgtagg tggcttgctt ctggcgcacc ggcgcggcgc gcagcggcca cgcatcgggg
     1081 tcgaaacgcg tgacgttgcg cgtgtaatag ttgaccccga cgaagtcgag cggctgattg
     1141 atgagctcga gatccccggc cggccagctc ggccaggcct cgccgaaaat ctcggcgagc
     1201 tcatcgggat accggccgag cagcatcgga tcgaggaatt ggcggttcat ataggcatcg
     1261 gcccgtgcgg tggcggcacg atcggccgcg tcttccgacg ccgcatactt gggctcgaga
     1321 ttgacgacga cgccgatgcg atgcttgcct tcgctgcgat aggctttgac ggcgccccca
     1381 tgcgcgcgca tcagctggtg ggccgcaatg ggcgcctcga agcggttgcg atgtccgggc
     1441 gccagcgcgc cgtgcaggta gccgccatcg gtcacgaccc acggctcgtt cagcgtcgcc
     1501 cacagcttga cccggccgtc gagccggcga aaggctgccg acgcgtagtc ggcaaaccat
     1561 ccggcgatat ccggattgag ccagccgccg agatcgtcga gcgcggcagg caggtcccag
     1621 tgaaacagcg tgaccatcgg ctcgatgccc tgctgcagca gcgtatcgac cagccgttca
     1681 taaaagtcta gtcccgcagc attgaccggt ccccggccgc tcggcagcac gcgactccac
     1741 gaaatgctga accgataggc gttcaggccg atctcgcgca tcaaggcgac gtcttcgcga
     1801 tagcgccgat agtggtcgca cgcgacgtcg ccggtgtcgc cgtcgcggac catgttcggc
     1861 gtgtggcaaa agcgctgcca gatgctcggg ccggcgccgt cggcgagcgg cgagccttcg
     1921 acctggtagg cggaagtagc gccgccccac agaaagtccg ccggaaattc gattcgtctg
     1981 ctcatgggcg ttccgttttc ttgttggtag tcatcgtccg tcgatcagca cgcgggcggg
     2041 caccgcgcca acgtgcaatt ctgcaccgga aaaagtcgcc ggcttgccgt tgatggtcgc
     2101 cgaccgtggc ggcttgtcgc ccggccagac gaagataaag ccgcccggcg gcattccaga
     2161 ccctgccgcc aggcgcaatg tcacccgatc gccgtcacgg cgcagcgtat agctcaagcg
     2221 tccgtacggc gtgcgcaggt tctgcagcgc aacgccgcct tggtcgagcc aatcgggcgc
     2281 gatgcccgcg gcgagcacga cggcatggtc ggcgtcacgc tcgtacgcga acagatccag
     2341 cgtcgcgcga atgaaatcgg aggctaccca ggcgtgcggc atgtcgccga cgaagcgcgg
     2401 ctgacgcgga tcgcggccga cgacctcggg ccactggttc cacgccagcg ggcgccggtc
     2461 ggcgagaaag aacgcgagca gcgcctgcgc gcggtctcgc cagcctagcc gtaccaaggt
     2521 cccgacgatg cgcatctcgt aaggcgtgta cacatcccac gccgtttt
//
LOCUS       DATANS010000319         3173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_15831, whole genome shotgun sequence.
ACCESSION   DATANS010000319 DATANS010000000
VERSION     DATANS010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15831"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1731)
                     /gene="argS"
                     /locus_tag="VHT22_04605"
     CDS             complement(82..1731)
                     /gene="argS"
                     /locus_tag="VHT22_04605"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX3136865.1"
                     /translation="MVAEGIARVAPGATPPAIALERPKQAKHGDYSTNAALQLAKALK
                     RNPRDLAADLVAALPRSPLLARAEVAGAGFVNLYVSDAARQSVVARILDEGDRYGSSA
                     RGGGEKVIVEFVSANPTGPLHVGHGRGAAVGDAIARLLAAQGFAVHREFYYNDAGAQI
                     MNLALSVQARIREAQGIAASFPEDGYQGEYIWEIARDYAAANPDDRAADDLDAIRRFA
                     VAILRREQDEDLTAFGIRFDHYYLESSLYDEGLADSTVQALIAAGKTYEHEGALWLKT
                     TDYGDDKDRVMKKSDGSYTYFVPDIAYHVTKWRRGYRRAITELGADHAGSLSRVRAGL
                     QALGLGIPSGYPDYVIHQMVLVMRGGEEVKLSKRAGSGVTLRELIDEVGRDAVRFFFL
                     LRKSDSQLTFDIDLARSQSEENPVYYVQYAHARVCSVLDKAGIDVAAAPQALIDADLS
                     PLQSSYEQALLNRLADFPEELEAAARELAPHLVTFYLKELAAEFHSYYNAEQFLVDDV
                     ALRKARLALVVAVGQVIRNGLAMLGVSAPVAMRKEAPNHED"
     gene            complement(1829..>3173)
                     /gene="priA"
                     /locus_tag="VHT22_04610"
     CDS             complement(1829..>3173)
                     /gene="priA"
                     /locus_tag="VHT22_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX3136866.1"
                     /translation="RQGLAAASGEAQLIVGTRLAVFAPAPHLGLIVVDEEHDASFKQH
                     EGVRYHARDVAVYRASRRDVPVILGSATPSLETYAQAMRGRYRWLKLPLRATAQTALP
                     PVRLVPTRAEGTVEGIAPALREAIAARLARGEQSLLFINRRGYAPALLCPGCGWKAPC
                     PRCAARLVVHRETAGLRCHHCGHAEALPHSCPNCGNQDLLPQGSGTQRLERALADLYP
                     AARILRIDRDSTQGKQTFATMREAVDAGEVDILVGTQMLAKGHDFARLTLVGVLGADN
                     ALYSGEFRATERLFALLEQVAGRAGRRDLPGEVLVQTDFPQHPLYVALAAHDFDRFAA
                     TLLAEREALALPPFAHLALLLAEARSPEPVQAFLAAASERGRELAAAAGACEVYSPTP
                     AALPRRAGLERAQVLVQSRDRGSLQAFLPRWRAELERLAERKVRWTLDVDPLGFG"
BASE COUNT          523 a         1093 c         1038 g          519 t
ORIGIN      
        1 accgagcacg acgccgccac gcatcgatcg gcgcgcccgg cgggcgcagc gttttctggt
       61 ctgtgcgaaa ttcatcgatc cctagtcttc gtggttcggc gcttccttgc gcatcgctac
      121 cggcgcgctg acgcccagca tcgcgagccc gtttcgtatc acctgtccga cggcgacgac
      181 cagagcgagg cgcgccttgc gcaaagcgac atcgtcgacg aggaactgtt cggcattata
      241 gtagctgtga aattcagccg ccaattcctt cagatagaag gtcaccagat gcggcgccag
      301 ctcgcgcgcc gcggcttcca gctcctccgg aaaatccgcc aggcggttca atagcgcctg
      361 ctcgtaggaa ctttgcagcg gtgacaggtc cgcgtcgatc agcgcctgcg gcgcggcggc
      421 gacgtcgatt cccgccttgt cgagcacgct gcaaacgcgc gcgtgcgcgt attgcacgta
      481 gtagaccgga ttttcctcgc tctgcgagcg agccaggtcg atgtcgaatg tcagctgcga
      541 atcggacttg cgcagaagga agaaaaagcg caccgcgtcg cggccgactt cgtcgatcag
      601 ctcccgcagc gtgacaccgc tgccggcgcg cttgctcagc ttcacttcct cgccgccgcg
      661 catcaccagc accatctggt gtattacgta gtccgggtat ccgctcggaa tgcccaggcc
      721 cagcgcttga agcccggcgc gcacccgcga cagcgagccg gcgtggtcgg cgcccaactc
      781 ggtgatcgcg cgcctgtagc cgcgccgcca cttggtgacg tggtaggcga tatcgggaac
      841 gaaataggtg tagcttccat ccgacttctt catcacccgg tccttgtcgt cgccgtaatc
      901 ggtggtcttg agccagagag ctccttcgtg ctcgtaggtc ttgcccgccg cgatcagcgc
      961 ctgcacagtg ctgtcggcaa gcccctcgtc gtacagcgac gactccaggt agtagtggtc
     1021 gaaacggata ccaaatgcgg tgagatcctc gtcctgttcg cgacgcaata tcgccacggc
     1081 gaaacggcgg atcgcgtcca gatcgtcggc ggcgcggtcg tcaggattcg cggcagcgta
     1141 gtcgcgcgcg atctcccaga tgtattcgcc ctggtagccg tcctcgggaa agcttgcggc
     1201 gatgccttgc gcttccctga tccgcgcctg caccgacaag gcgagattca tgatctgcgc
     1261 accggcgtcg ttgtaataga actcgcgatg aacggcaaaa ccctgggcgg cgagcaggcg
     1321 cgcgatcgcg tcgccgaccg ccgcaccgcg gccgtgcccg acatgcaacg ggccggtggg
     1381 gttggccgac acgaattcga cgatcacctt ttcgccgccg ccgcgcgcag agctgccgta
     1441 gcgatcgcct tcatccagaa tccgcgccac gaccgattgc cgcgcggcat cggagacgta
     1501 cagattgacg aatcccgcgc cggcgacttc ggcgcgcgcg agcagcggcg atcggggcag
     1561 cgctgccacc aggtcggccg ccagatcgcg cggattgcgt ttgagcgcct tggcgagctg
     1621 cagcgccgcg ttggtcgaat aatcgccatg cttggcctgc ttcggccgct cgagcgcaat
     1681 cgccggcggc gtcgcgcctg gcgcgacgcg ggcgatgccc tcggcgacca tcgccgacag
     1741 ttctgttttg agttgggtca aggccgcgtt tcttgggagc aaactgcgaa ttataacgcc
     1801 cggacagtcg cggacctgag cgtgcgcttc agccgaaccc cagcggatcg acatcgagcg
     1861 tccaccgcac tttgcgctct gccagccgct cgagctccgc gcgccatcgc ggcaggaatg
     1921 cctgaagcga accgcgatcg cggctttgca cgagcacctg cgcgcgttcc aatcctgccc
     1981 ggcgcggcag cgccgcgggg gtcggagaat acacttcgca cgcaccagcc gccgcggcga
     2041 gctcccggcc gcgctccgac gccgcggcga gaaatgcctg caccggttcg ggcgatctgg
     2101 cctcggccag cagcagcgcc agatgcgcga acggtggcag tgcgagtgct tcgcgctcgg
     2161 caagcagcgt cgccgcgaag cggtcgaaat cgtgggcagc gagcgcaacg taaagcggat
     2221 gctgcggaaa atctgtctgc accagcactt cgccgggcag gtcccggcgg ccggcgcggc
     2281 ccgcgacctg ctcgagcagc gcgaacagac gctcggtcgc gcgaaattcg ccgctgtaaa
     2341 gcgcattgtc ggcgccgagg acgccgacca gggtcaggcg cgcgaagtcg tgtcccttgg
     2401 ccagcatctg cgtgccgacc agaatatcga cctcgcctgc atcgacggcc tcgcgcatcg
     2461 tcgcgaatgt ctgcttgccc tgcgtgctgt cgcgatcgat gcgcaggatc ctcgctgccg
     2521 gatacaggtc ggcgagcgcc cgctcgaggc gctgcgtgcc cgacccttgc ggcagcaagt
     2581 cctgattgcc gcaattcggg caggaatgcg gcagcgcttc cgcgtggccg caatgatgac
     2641 agcgcaggcc cgcggtttcg cggtgcacga ccagcctcgc cgcgcaacgc gggcatggcg
     2701 ctttccatcc gcagccggga cacagcagcg ccggcgcgta accgcgccgg ttgatgaaca
     2761 gcagcgactg ctcgccgcgc gcgaggcgag cagcgatcgc ctcgcgcagc gccggtgcga
     2821 tgccctcgac ggtcccctcg gcgcgggtcg gcaccaatcg taccggcggc agcgcggtct
     2881 gcgcagtggc gcgcagcggc agcttcagcc agcgatagcg gcccctcatc gcctgagcat
     2941 aggtttccag cgacggcgtt gcgctgccga ggatcaccgg cacgtcgcga cggctggcgc
     3001 ggtagaccgc gacgtcgcga gcgtgatagc gcacgccctc gtgctgcttg aacgaggcat
     3061 cgtgctcctc gtcgacgacg atcaggccca gatgaggcgc cggcgcaaaa acggcgagcc
     3121 gcgtcccgac gatcagctgc gcctcgccgc tcgccgccgc cagcccttgg cgg
//
LOCUS       DATANS010000320         2449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_15879, whole genome shotgun sequence.
ACCESSION   DATANS010000320 DATANS010000000
VERSION     DATANS010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15879"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..708)
                     /locus_tag="VHT22_04615"
     CDS             complement(16..708)
                     /locus_tag="VHT22_04615"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX3136867.1"
                     /translation="MNGSALLRTAAIALALCASAVQAQQYPNRAIRLVVPFAPGGSTD
                     VLARIVGQRMTESMGQAVIIDNRPSAGGTTGSDLVAKALPDGYTLVIGSIATMAIAKA
                     MYANLPYDPQRDFEHIGLWVTFPLALVVPAGSPLTSLKDLIEQAKAKPGALRYGSQGI
                     GTSAHIFGDLMSSMAGVKVVSVPYKGGGPALTGVLVGEVNYAMIAVSTALAQVNAGKL
                     HALAPLRASGFW"
     gene            complement(705..1502)
                     /locus_tag="VHT22_04620"
     CDS             complement(705..1502)
                     /locus_tag="VHT22_04620"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3136868.1"
                     /translation="MPQVAIADGEIHYEVTGDGHPLILVSGLNGVARYWEPQIAAFSA
                     RYRVITYDQRGTGTSDHKQHEFSIDQMARELSALMDALKIERAHLVGLSTGGAIGQTL
                     AIEQPQRIARLVLVSTWTHCDPWFRRLFEARRRMYELCGPELHAMFHPLFLYPPDYVN
                     AHDAEIAEEQKRAPTKSAPVEASIGRIDALLKFDRRPGLARINAPTLIVAGDNDYITP
                     SYHAQAIAGAIPGSKLVVFNGGGHSLSKTRPEEFNRTVLDFLGAGKA"
     gene            complement(1567..>2449)
                     /locus_tag="VHT22_04625"
     CDS             complement(1567..>2449)
                     /locus_tag="VHT22_04625"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX3136869.1"
                     /translation="MIVPYPPGGSVDFTGRELAAKLSELWGQQVVLDNRGGAGSTLGH
                     SLGAKAIPDGYTLLLGTSAGLVVSPALGTKLNYDSLKDYAPIGLAVYAPFALTLHAGV
                     AANTTQEFIDLAKANPGKINFSSPGTGTPNHLGGELLKALAHIIIVHVPYKGGGPALV
                     DLISGQVQMTFSGVPQILPHVKAGRVKIIAIGHPTRIKSLPDTPPVADVLPGFNNTSW
                     YGLLAPAGTPPALVNQINAAMNKVLVQPEFGQRLLLQGVEPVTSTPQGMHDMIRNELA
                     RWRKVIKEAGITVETAQ"
BASE COUNT          405 a          805 c          782 g          457 t
ORIGIN      
        1 cgcccgcgcc tcccgtcacc aaaacccact tgcccgcaat ggggccagcg catggagttt
       61 gcccgcgttg acctgcgcga gcgcggtcga gaccgcgatc attgcgtaat tgacctcgcc
      121 gacgagcacc ccggtcagcg cgggcccgcc acctttgtac ggcacgctga cgaccttgac
      181 gcccgccatg ctgctcatca ggtcgccgaa tatgtgcgcg gatgtgccga taccctgcga
      241 gccatagcgc agcgcgccgg gcttcgcctt tgcctgctcg atcaggtctt tcaaactcgt
      301 gagcggcgat cccgcgggca ccacgagcgc cagcgggaat gtcacccaca gaccgatgtg
      361 ctcaaaatct cgctgcggat cgtacggcag gttcgcgtac atcgccttgg cgatcgccat
      421 cgtggcgatg gacccgatca cgagcgtgta gccatcgggc agcgccttcg ccacgaggtc
      481 gctccccgtg gtgccgccgg cggacggccg attgtcgatg atcaccgcct gtcccatgct
      541 ctcggtcatg cgctgtccga cgatgcgggc gagcacgtcg gttgagccgc ccggcgcgaa
      601 cggcacaacg aggcggattg cgcggttcgg atattgctgg gcctgcactg cggaggcgca
      661 gagcgccagt gcaattgccg ccgtccgcag cagggcgctg ccgttcacgc tttccccgcc
      721 cccagaaaat ccagcaccgt gcggttgaat tcctccggtc gcgtcttgga cagcgaatgg
      781 ccgccgccat tgaacacgac aagctttgag cctggaatcg cgccggcgat cgcttgcgca
      841 tgatagctcg gtgtaatgta gtcattgtcg ccggcaacga tcagggtcgg cgcgttgatg
      901 cgcgccagtc ccgggcgacg gtcgaattta agcagcgcat cgatgcggcc gatcgatgcc
      961 tcgacgggtg cggacttcgt cggcgcgcgc ttttgctctt ccgcgatctc agcgtcgtgc
     1021 gcgttgacgt aatccggcgg atacaggaac agcggatgga acatcgcatg cagttccggc
     1081 ccgcatagct cgtacatgcg gcgccgcgcc tcgaagagcc gccggaacca cgggtcacaa
     1141 tgcgtccacg tgctgaccag cacgagccgc gcaatgcgct gcggctgctc gatcgcgagc
     1201 gtctggccga tggcgccgcc agtcgacagg ccgacgagat gcgcgcgctc gattttcagc
     1261 gcatccatga gcgcggagag ctcgcgcgcc atctggtcga tcgagaattc gtgttgctta
     1321 tggtcgctgg tgccggtgcc gcgctgatcg taagtgatca cgcgatagcg cgcggagaac
     1381 gcggcaattt gcggctccca gtagcgcgcc acgccattga gcccgctgac gaggatcagc
     1441 ggatgcccgt cgcccgtaac ttcgtaatga atttcgccat cggcgatcgc aacctgcggc
     1501 atcgtgcatc tcctcgctgt tttcgctgag cttccggcat tttaccggca acgcccggct
     1561 gcgatttcat tgtgccgttt cgacggtgat gcccgcttcc ttgatgacct tgcgccagcg
     1621 cgcgagctcg ttgcggatca tgtcgtgcat gccctgcggc gtgctggtta ccggctcgac
     1681 gccctgcagc agcagacgct ggccgaattc cggctgcacc agaactttat tcatcgccgc
     1741 gttgatctgg ttgacgagcg ccggcggcgt gccggccggc gcgagcaatc cgtaccacga
     1801 tgtgttgttg aatccgggca gcacatcggc caccggcggc gtatcgggca acgacttgat
     1861 gcgcgtcggg tggccgatcg cgatgatttt tacgcggccg gctttgacgt gcggcaagat
     1921 ctgcggcaca ccggagaacg tcatctgcac ctggccgctg atcaggtcga ccagcgccgg
     1981 cccgccgcct ttgtacggca cgtgcacgat aatgatgtgc gcgagcgcct ttaaaagctc
     2041 gccaccaaga tggttcggcg tgccggtccc gggcgacgag aagttgattt tgcccgggtt
     2101 ggctttcgcc aggtcgatga attcctgcgt ggtattggcc gccacgccgg cgtgcaacgt
     2161 cagcgcgaac ggcgcataga ccgcgagccc gatcggtgcg taatccttga gcgagtcgta
     2221 attaagcttg gtgccgagcg ccgggcttac caccaggccc gccgacgtgc cgagcagcag
     2281 cgtatagccg tcaggtatcg ccttggcgcc gagactatgc ccgagcgtcg agcccgcgcc
     2341 gccacggttg tcgagcacga cctgctggcc ccacagctcg cttaacttcg cggcgagctc
     2401 gcgcccggta aagtcgaccg agccgcccgg cggatacggc acgatcatg
//
LOCUS       DATANS010000321         1699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_562568, whole genome shotgun sequence.
ACCESSION   DATANS010000321 DATANS010000000
VERSION     DATANS010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562568"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VHT22_04630"
     CDS             <1..912
                     /locus_tag="VHT22_04630"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX3136870.1"
                     /translation="REVGHILQRFDAGYLLLAIAVVTIDRILMTFKWTLLLRAQGYRL
                     PLLQGVTIYCTAMVWGLALPATVGADAIRAVMVTKRGFNGTDVVASIVIERMVGFVLA
                     LALGLASLAILRTLGVLDARFASALYLGAAMLVGVTALLVAALNEKLVANVVARLPRV
                     VRESKVMHYLDRFAAAYRALGSARGTIASFSLLTVLEQVFSVLFPWTLALGLGVDVNL
                     LLLLGVLPISTLISRLPISFDGLGVYEAVFVGLLVLAGIGADAALAIAISGRIIQLFA
                     FLPWWITYVARSGAVRPPAASKELPTN"
     gene            complement(999..>1699)
                     /locus_tag="VHT22_04635"
     CDS             complement(999..>1699)
                     /locus_tag="VHT22_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010057461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEX3136871.1"
                     /translation="VYSEAVVERLARMKTAAPDFRLFWDNAYAVHHLTAERVRIANID
                     TACARHGNPNRAFIFGSTSKVTLAGSGFGLFGGSKDNVAWYLKRMEKRTIGGDKLNQL
                     RHVQLLQNAEGVERLMERHRAIIGPKFAQVRATFEAHLAGTGVARWTDPRGGYFITLD
                     VLDGCAKQVVKFAKEAGVELTPAGATHPHGNDPRDSTIRIAPTFPEPAEIAQAAEGVV
                     LCVLLAAAEKLLAK"
BASE COUNT          263 a          546 c          552 g          338 t
ORIGIN      
        1 cgcgaagtcg ggcacatcct gcagcgcttc gacgcgggct atctgctgct cgcgatcgcg
       61 gtcgtcacga tcgatcgcat tctgatgacg ttcaaatgga cgctgctgct gcgggcgcag
      121 ggttatcggc tgccgctgct gcaaggcgtg acgatctact gcacggcgat ggtctggggc
      181 ttggcgctgc cggcgacagt cggtgccgat gcgatccgcg cggtgatggt gacgaagcgc
      241 ggtttcaacg gcacggacgt ggttgcctcg atcgtaatcg aacgcatggt cggcttcgtg
      301 ctggcgctcg cgctcggtct cgcgagcctg gcgatcctgc gcacgctcgg cgtgctcgat
      361 gcgagattcg ccagcgcgct gtatctcggt gccgcgatgc tggtcggcgt gacggcgtta
      421 ctggtcgcgg cgctcaacga gaagctggtc gccaacgtcg tcgcccggct gccgcgcgtc
      481 gtgcgcgaat cgaaagtcat gcactacctg gaccggttcg ccgccgctta tcgcgcgctc
      541 ggcagcgcgc gcggcacgat tgccagcttc agcctgctga ccgtgctgga gcaggtattc
      601 tcggtgttgt ttccctggac gctggcgctg ggcctcggcg tcgacgtcaa cctgttgctg
      661 ctgctcggcg tgctgccgat cagcacgttg atctcgcgcc tgccgatttc cttcgacggc
      721 ctcggcgtgt acgaagccgt gttcgtgggt ttgctggtgc tggcgggaat cggcgcggat
      781 gcggcgctgg cgatcgcgat cagcggccgc atcattcagc tgttcgcatt cctgccgtgg
      841 tggatcacgt acgtggcgcg ctcgggcgcc gtgcgtccgc cggcagcttc gaaggagtta
      901 ccgaccaact gaagtgacac aggtgacaca ggtgatgaaa gtgacacaag gaagacccgt
      961 tctgacctct gtcacccatg tcacttgtgt cacctgtgtc acttcgccag taacttctcc
     1021 gccgcggcca acaacacgca caggacaaca ccttccgctg cctgcgcaat ctctgccggc
     1081 tccgggaacg tcggtgcgat gcgaatagtg ctgtcgcgcg ggtcgttacc gtgcgggtga
     1141 gttgcacccg ccggcgtgag ctcgacaccg gcttccttcg cgaacttgac gacctgcttc
     1201 gcgcagccgt cgagcacgtc gagcgtgatg aagtatccgc cgcgcggatc ggtccaacgc
     1261 gcaacgccag tgcccgcaag atgcgcttcg aacgttgcac gcacttgcgc gaacttcggt
     1321 ccgatgatcg cgcgatgacg ctccatcaac cgctcgaccc cctcagcgtt ctgcagcagc
     1381 tgcacgtgac gcagctggtt gagcttgtcg ccgccgatcg tgcgcttctc catgcgcttc
     1441 aaataccacg cgacgttgtc cttcgagccg ccgaacaggc cgaaacccga gccggcgagc
     1501 gtcaccttcg aagtcgagcc gaaaatgaac gcgcgattcg ggttgccgtg acgcgcacag
     1561 gcggtgtcga tgttcgcgat gcggacgcgt tccgccgtca ggtgatgcac ggcgtacgcg
     1621 ttgtcccaga acaagcggaa gtccggcgct gcggtcttca tgcgcgcgag ccgttcgacg
     1681 actgcctccg agtacaccg
//
LOCUS       DATANS010000322         1882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_394836, whole genome shotgun sequence.
ACCESSION   DATANS010000322 DATANS010000000
VERSION     DATANS010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394836"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..>1882)
                     /locus_tag="VHT22_04640"
     CDS             complement(187..>1882)
                     /locus_tag="VHT22_04640"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3136872.1"
                     /translation="VAAGDSRAQTPAESKQADGTTAPEPPAAADDLTPEVLYRILVGD
                     VAMQRGDAALAARAYFEAARESRDPVLARRATEIALFARQRTLALEAAKLWQQIDPKA
                     DRARQMVASLSLSGAGSDLKAELQRVLIEAAANDKSLGDAFLQLNQALAAQTDKAAAF
                     RLVQDLARPYPKVPEAWFAVALAGYNTKLTDPAITAAATTAADRALELKPGWDRAALI
                     KADILAKGSPDAAITFLKQFLADSPDARAVAGALAQIYVEQKRFAEARAIFQQLWDEE
                     PEDREFEFAVAAISLQMKDYATAQRLLEELDKVGYGDKGVVPFYLGQLAEETKRYDEA
                     IARYREVTEGDRAWLAKLRIGAMIAKKGDIAAARSYLDGLTPESAERRVELAQAEAQL
                     LRDAGDYKTAYAVLTAALERQADSVDLLYDVAMVAEKLDRIDEVESRLKRLIELKPDN
                     AQALNALGYTLVDRTPRTAEGLKLIEKALQIAPDDPFILDSMGWANYRLGKLDDSEKF
                     LRRAFGDQADPEIAAHLGEVLWAKGERQRATELWQSQLKASPDNPVLIETMRRLAH"
BASE COUNT          272 a          648 c          626 g          336 t
ORIGIN      
        1 gttgccacgc cgcgccgaca ggcgtccggt caactcgaac gccgccgcct gtgccgctag
       61 cgggcttgcg ggcgcggacc ggtcgaccag cgccggggcg caagagcaaa gcagtaccgc
      121 gcccgccgcg gcgactgcgc gagcgcacat gccgcgcagc ctgggcatcg acctggcgac
      181 cggacgtcag tgcgccaggc ggcgcatcgt ctcgatcagg accggattgt cgggcgaggc
      241 cttgagctgc gattgccaca actcggtggc ccgctggcgc tcgcctttcg cccacagcac
      301 ttcgcccaga tgcgcggcga tctcggggtc cgcctggtcg ccgaatgcgc gccgcaggaa
      361 tttttccgaa tcgtcgagct tcccgagccg gtagttggcc catcccatgc tgtcgagaat
      421 gaacggatcg tccggcgcga tctgcagcgc tttttcgatc agcttcagtc cctccgcggt
      481 gcgcggcgtg cggtcgacca gcgtgtagcc gagcgcattg agcgcctgcg cgttgtccgg
      541 cttgagctcg atcagccgct tgagcctcga ctcgacctcg tcgatgcggt cgagcttctc
      601 ggcgaccatc gcgacatcgt agagcaggtc gaccgaatcg gcctggcgct ccagggccgc
      661 ggtaagcact gcgtacgcgg tcttgtagtc gccggcatcg cgcaacaact gcgcttccgc
      721 ttgcgcgagc tcgacgcgcc gctctgccga ctccggcgtc agtccgtcca gatagctgcg
      781 cgccgcggcg atgtcgcctt tcttggcgat catcgcgccg atccgcagct tggcgagcca
      841 ggcgcgatcg ccttcggtga cttcgcggta ccgggcgatc gcctcgtcgt agcgtttggt
      901 ttcttcggcc agctggccga ggtagaacgg cacgacaccc ttgtcgccgt aaccgacctt
      961 gtcgagctct tcgagcagtc gctgcgcagt cgcgtaatcc ttcatctgca gcgatatcgc
     1021 cgcgacggcg aattcgaatt cgcggtcttc cggctcctcg tcccacaact gctggaagat
     1081 cgcgcgcgcc tcggcaaagc gcttttgctc gacgtagatc tgcgccagcg cgccggcaac
     1141 tgcgcgcgcg tcagggctgt ctgcgagaaa ttgcttcagg aacgtgatcg ccgcatccgg
     1201 cgatcctttg gccagaatgt cggccttgat cagggccgca cggtcccatc cgggcttaag
     1261 ctcgagcgcc cggtccgccg cagtcgtcgc ggcggcggtg atcgccggat cggtcagctt
     1321 ggtgttgtaa ccggcgagcg cgacggcgaa ccacgcttcc ggaaccttcg gatacggtct
     1381 cgccagatcc tgcaccagac gaaacgccgc ggccttgtcg gtttgcgcgg ccaacgcctg
     1441 attgagctgc aggaaagcgt cgccgagcga tttgtcattc gccgccgctt cgatcagcac
     1501 gcgctgaagc tcggccttga gatcggagcc ggcgcccgag agggagagcg acgcgaccat
     1561 ctgtcgcgcg cggtcggcct tcgggtcgat ctgctgccag agcttcgccg cttccagcgc
     1621 cagcgtgcgc tgccgggcaa acagcgcgat ctcggtggcg cggcgggcga gtaccggatc
     1681 gcgcgactcg cgcgcggctt cgaaatacgc gcgtgccgcc aatgccgcat cgccgcgctg
     1741 catcgcgaca tcgccgacca ggatgcgata aagcacttcc ggggtcagat cgtcggccgc
     1801 agcggggggc tcgggtgcgg tcgtcccgtc ggcttgcttg gactcggcgg gcgtctgcgc
     1861 ccggctgtct ccggccgcca cg
//
LOCUS       DATANS010000323         8329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_58735, whole genome shotgun sequence.
ACCESSION   DATANS010000323 DATANS010000000
VERSION     DATANS010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 8329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8329
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58735"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1828
                     /locus_tag="VHT22_04645"
     CDS             131..1828
                     /locus_tag="VHT22_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3136873.1"
                     /translation="MALDRIAILDGFGPDDVLSWHRGRPVAGAPFCGAARRLAARLPP
                     ARYAVNLCEETPNFLLGSVAALLSGQTLLMPPSRLAQSLADIRLSHPDSYCLTDSEVA
                     GTEATLCVSVDVASEVDASQSDWPPPVADAAHVAAVLFTSGSTRAPWPHAKTWGELVA
                     GARTLMASFGRPPSGTGIIGTVAPQHMFGFETTVMLPLQSGAAVLEARPAYPADLAEA
                     LTVAARRNIGTLWLMTTPLQLRAFHRGLPALRGLNRIITATMPLARDLAQGVERDWEV
                     RLEEIFGCTEGGILATRRPAETPMWTPAAGLEFSVDADGSARVQGAHLPRPLSLSDRI
                     RMVESPAGATSGAFELVGRDEDVVKIAGKRASLSGLADQALAIAGVRDAVFFVPGPGS
                     VRLAAILAAPERSLDDLRAELARRIDAAFLPRPLVLVETMARDSNGKLPRAALEKMLG
                     AATTSRAQAPAPEDAVLERAWCVPADHPALPGHFPGWPVVPGVMLLDAVEAMLADHGY
                     RLRECMQVKFIAPVAPVSPLTLRLEISDGQHVRFLIRSAGQTAVTGVLRCSRVEAST"
     gene            1825..2733
                     /locus_tag="VHT22_04650"
     CDS             1825..2733
                     /locus_tag="VHT22_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HEX3136874.1"
                     /translation="MIVRPLWIDQAERGSRWLMQLMVYLALLLGRAAGRVLLYPVCAY
                     FLVFSRRARLASTHYLSRVLERRAGVRDVFRHYLCFATTILDRTYLMAKRIEYFDCHV
                     NGADALLAVAARREGCLLFGAHLGSFEMLRVLAVTGCPVPVQILMYEENAGKLNSVLR
                     ALNPKSAMQVIALGRPQTMLLVAEALARGEIVALLADRVVAGDKFQRCPFLGADAPFP
                     EGPMILAAALRVPVILFSAIYRGGRRYDIRFEPFTERIILPRAGRAEVLRTECARYAA
                     WLQSRCRDAPYNWFNFYDFWKHDQGC"
     gene            2717..3328
                     /locus_tag="VHT22_04655"
     CDS             2717..3328
                     /locus_tag="VHT22_04655"
                     /inference="COORDINATES: protein motif:HMM:NF039988.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX3136875.1"
                     /translation="MTKVASAKRAAAFCRLLCLAALSLFPYAISIGAAPLDAAELMAL
                     LSQVDKSSVNFEETKHLAALTAPLVRRGTMRYVRPERLEMQVDKPYFERLEVIGDRLT
                     IENRDGKRQIDLASQPLAGVLVEGLRAVLAGDLPGLARRYRYRFEGELGAWKLELQPL
                     DKGAAAAVRRIDIEGSKAQLSRIAIEEAQGDRTHLVLSPGPVQ"
     gene            3325..5679
                     /locus_tag="VHT22_04660"
     CDS             3325..5679
                     /locus_tag="VHT22_04660"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX3136876.1"
                     /translation="MNARGLSVVSWLVLVILALAYVTTRVSIESDFSVFLPRGLNDAQ
                     RTFLAQLRNGSVSRLVLIALDNDEPQSLAKLSASLAAALEKDPAFTYVNNGGSALAQR
                     ELNSIAAVRYVLSDRVTADRFSPEGLRTALEKRLEGLAGSAGAFEKTLLASDPTGETL
                     HVLQRLTPDRQPRRIDDAWFDETGRSALLVVETRAAGSDLAGQDAALKRLGAAFALVR
                     GDGAARMQYSSPGAMAVESRALITADVTWLSLLSTVLILLILGSVYRSLLVVLVCVLP
                     ALSGLLAGIVAVNFWFGTVHAIALGFGATLLGEAVDYPSYLLTQRRPGEAMRDTLARM
                     LPMLRLAVATTTFGALALLMSHFSGLAQLGLLTVVGVVVAGLVTALVLPHLIPPGWHP
                     RLATARPARLPDAFAGIGPRAAIVLIIAATVMALAANRPWWDDDLAHMNPLPPAVLAQ
                     DRQLRQALNAPEVGSLLVASGATREEVLGIAESLRPALEAWQAAGDIGSFTLVSDYLP
                     SATTQTMRRAALPEAPRLRSNLDAALAGLPFRADTFEPFLRDVEQARAAKPLTSEALQ
                     GSALGLKVDALLSEDGGRWSAVVPMTRVTSPGMMSGKVAALRVPGAQWIDLRTQAVAM
                     VTAYRWQTLTYTAMGILMIYGALAYGLKSWRRAIEVLFPVVIALLLTTGTLVASGQPV
                     SIFHLVALLLTLGIGTNYALFLARASQPGAERSGTLRTLAVVGGTTLCAFGALTLSST
                     PVLHAIGMTVCLGVLYSLLLSLLLLWPPPLPSTLPSTSTSPIDE"
     gene            5676..6890
                     /locus_tag="VHT22_04665"
     CDS             5676..6890
                     /locus_tag="VHT22_04665"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEX3136877.1"
                     /translation="MTPLPLAAVTLTNACGVGLDEIRRALRTGSSGLRQNDFEPARGL
                     DTWIGRVDGLESEALAPDCVAHDCRNNRLARLSLDQDGFVAAVDAARSRYGEGRVGVF
                     IGTTTSGILSTELAYRSGSLASGGPSPPEDEPSAGLSPELYRYKHSMYATTMFVRSYL
                     SLSGYAMTISTACSSSAKVFAAAARAIDAGLCDAAVVGGVDSLALSTLFGFHSLELLS
                     RRPCRPFASDRDGISIGEGAGFALLDPQAESPVRLLGAGESADAYHMSSPHPEGAGAA
                     AAMRKALSGAGLVGRDIDYINLHGTASRANDLAEDRGVVSAVGKEPAASSTKGWTGHL
                     LGAAGITEAVISALSLIDQWIPGTLNCERVDPALHSNLLVDSQTMRLRHVMSNSFGFG
                     GSNCSLVFGLGQ"
     gene            6887..7696
                     /locus_tag="VHT22_04670"
     CDS             6887..7696
                     /locus_tag="VHT22_04670"
                     /inference="COORDINATES: protein motif:HMM:NF025100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEX3136878.1"
                     /translation="MKPLAITGVGVCGAGLHDWTQAKPVLDGTARFEARAVDKFDLAC
                     LPPIERRRANESSRLAMVAALQAIEHVSIAERERLSTVFSSADGDTEVLAHLLAKLAT
                     SDIMLSPTLFHNSVFNAPAGYWSLGCKAQAASTTVCAGRASFAVGLCEVNSQVLASGE
                     AVLYIAYDMAFPEALKRLGRTTMAFACALLLEPTSDGAPGRFGMIEGWQREVGARETD
                     AGLQALAEGFAGNAAAAALPLLTAIATQAGTEIRLPYLDDSRLHLRYSPWS"
     gene            7687..8124
                     /locus_tag="VHT22_04675"
     CDS             7687..8124
                     /locus_tag="VHT22_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HEX3136879.1"
                     /translation="MVLNQDAIRGLIPHRASMCLLDTVEYWDAERVVCRTSQHRSPGN
                     PLRANARLSSIHGIEFAAQAMAVHGGLLAPNGAAPSVGLLVSLRDCVLHCERLDVLDS
                     PLTVEARRITGNNGLTMYGFSVTANTLVIEGRASVLLQREAAG"
     gene            8121..>8329
                     /locus_tag="VHT22_04680"
     CDS             8121..>8329
                     /locus_tag="VHT22_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3136880.1"
                     /translation="MKRALVTGGSGSIGAAVCRTLASAGHHVLVHTHSRPDKAAAIVT
                     AITEAGGSADAVQFDVADGDATALVL"
BASE COUNT         1281 a         2635 c         2830 g         1583 t
ORIGIN      
        1 tcggcatgct gttcgtgggt gagtatgttt accggcgcgt acgattttcc cactacgagc
       61 atgcatcccc gctccgattg ctgggcatag ttcgtcgcgc aatccgcgag ttgcgttgac
      121 gaggcgatcg atggcactcg accggatcgc aatcctggac ggcttcggac cggacgatgt
      181 gctcagctgg catcgcggca ggccggtcgc cggggcgccg ttctgcggcg cggcacgacg
      241 gcttgccgcc cggcttccgc cagcgcgcta tgcagtcaat ctgtgcgagg agacgccgaa
      301 ttttctcctc ggcagcgttg ccgcgctcct gtcgggtcag acgctgctga tgccgccgtc
      361 ccggttggcg caatcgcttg ccgacatccg cctgtcgcat cccgacagct actgcctcac
      421 cgattccgag gtagccggaa ccgaagccac gctttgcgtc agcgtcgacg ttgcgtcgga
      481 ggtcgacgcc agccaatctg actggcctcc gccggtagcc gacgcggcac atgtcgcggc
      541 ggtccttttc acgtcagggt cgacgcgcgc gccatggcca cacgcaaaga cgtggggcga
      601 gctggttgcg ggcgcccgaa ccttgatggc gtctttcggc cggccgccgt cgggcacggg
      661 catcattggc accgtggcgc cgcagcacat gtttggcttc gagacgacgg tgatgctgcc
      721 gttgcaatcg ggagcggcag tgctcgaggc tcggcccgcc tatcccgccg atctcgccga
      781 agccctgacc gtcgccgccc gacggaacat cggtacgcta tggctgatga ccacaccgct
      841 gcaattgcgc gcctttcatc gcgggctccc cgcgctgcgc ggattgaatc gcatcatcac
      901 ggcgacgatg cctctggccc gcgatctcgc gcaaggcgtc gagcgcgact gggaagtgcg
      961 actggaggaa atcttcggct gtaccgaggg gggaatcctt gcgacccgcc gcccggccga
     1021 aacaccgatg tggacgcctg ccgcagggct cgaattttcg gtcgacgccg acggcagcgc
     1081 gcgcgtgcag ggggcgcatc tcccgcgacc gctctctctc agcgaccgga ttcgcatggt
     1141 cgagtcgcct gcgggtgcga cgagcggtgc cttcgagctg gtcggccgcg acgaagacgt
     1201 ggtcaagatc gcgggcaaga gagcatcgct gagcgggctt gccgaccagg cgttggcgat
     1261 cgctggtgta cgcgacgcag tcttcttcgt ccccggaccg ggatcggttc ggctggccgc
     1321 gatcctggcc gcgccagaac gttcgctgga cgatttgcgc gccgagcttg cacggcggat
     1381 cgacgcggcg ttcctgccgc gtccgctggt gttggtggaa acgatggcac gcgattcgaa
     1441 tggcaagctg ccgcgggcag cactcgaaaa aatgctcggt gccgccacga catcacgcgc
     1501 tcaagcgccg gcgccggaag atgccgtcct cgagcgcgcg tggtgtgtgc cggccgatca
     1561 tccggcgctg ccgggccatt ttcccggctg gcccgtcgtt cccggcgtca tgttgctcga
     1621 tgccgtggaa gcgatgctcg ccgaccacgg ctatcgcctg cgcgaatgca tgcaagtgaa
     1681 attcatcgcg ccggttgcgc cggtctcgcc gctgaccttg cgactggaga tctccgatgg
     1741 ccagcatgtg cgctttttga ttcgctccgc cggccagaca gccgtcaccg gcgtccttcg
     1801 ctgcagccgc gtcgaggcca gcacgtgatt gtgcggccgt tgtggatcga ccaggccgag
     1861 cgcggcagcc gctggctgat gcaactgatg gtatacctgg cgttgctgct cgggcgcgcg
     1921 gcggggcgtg tgctgctcta tccagtctgt gcctacttcc tggtgttttc ccggcgcgca
     1981 cggctcgcgt cgacccacta tctgtcgcgg gtgctggagc gccgtgctgg tgtgcgcgac
     2041 gtgtttcgtc attatctgtg cttcgcaacg acgatactcg accggacgta cctgatggcg
     2101 aagcgcatcg aatatttcga ttgccatgtc aacggcgcgg acgcgctgct cgccgttgcc
     2161 gccaggcgcg aaggctgcct gctcttcggc gcccatctgg gcagtttcga gatgctgcgc
     2221 gttctggcgg tgacgggctg cccggtgccg gtgcagattc tgatgtacga agaaaatgcg
     2281 gggaaactga acagcgtgct gcgtgctctc aatccgaaat cggcgatgca ggtcattgcg
     2341 ctcggaaggc cgcagacgat gttgctggtt gcggaggcgc tcgcgcgcgg agaaatcgtc
     2401 gcattgcttg ccgatcgcgt cgtcgccggc gacaaatttc agcggtgccc gtttcttggc
     2461 gccgatgcgc cgtttcctga ggggccgatg atcctcgcgg cggcattgcg ggtgccggtt
     2521 atcctgtttt cagcgatcta ccggggtggt cggcgttatg acattcgctt cgagccgttc
     2581 accgagcgca tcatcctgcc acgggccggg cgcgccgagg tgctgcgaac cgaatgcgcg
     2641 cgctacgcag catggctgca aagccgctgc cgcgacgcgc cttacaactg gttcaatttc
     2701 tatgacttct ggaagcatga ccaaggttgc tagcgcaaag cgcgcagccg cgttctgccg
     2761 gctgctttgc ctggctgcgc tgtctctctt tccgtacgcc atctccatcg gcgcggcgcc
     2821 gctcgacgcc gccgagctga tggccctctt gtcgcaggtc gacaagagca gcgtcaattt
     2881 cgaggagaca aaacacctgg cagcgcttac ggcgcccctg gtcaggcgtg gaacgatgcg
     2941 gtatgtccgg cccgaacgac tggaaatgca agtcgacaag ccctatttcg agcgtctcga
     3001 ggtcatcggt gaccgactga cgatcgagaa ccgcgatggc aagcgacaga tcgacctggc
     3061 aagtcagccg ctcgccggcg tcctggtcga gggcttgcgc gccgtgctgg ccggcgacct
     3121 tcccggcctt gctcgccgtt accggtatcg cttcgagggc gagctcggcg catggaagct
     3181 ggaactgcag ccgctcgaca agggcgcggc cgccgcggtc cggcgcatcg atatcgaggg
     3241 atcgaaagcg cagttgtcgc gaatcgcgat cgaagaggcc cagggcgacc ggacccatct
     3301 tgtcttgtcg cccggtcccg tgcaatgaac gcgcgcggcc tgagcgtcgt ctcatggctt
     3361 gttctcgtga tcctggcgct ggcctatgtg acgaccaggg tgtccatcga gtccgatttc
     3421 tcggtcttcc tgccgcgcgg actgaacgat gcgcagagga cgtttctcgc gcaactgcgt
     3481 aacggctcgg tgagccggct cgtcctgatt gcactggaca acgacgagcc gcagtcgctg
     3541 gcgaagctca gcgcctctct ggccgcagcc ctcgaaaagg atccggcgtt cacttacgtg
     3601 aacaacggcg gcagcgccct cgcgcaacgg gagctcaact cgatcgcagc agttcgctat
     3661 gtgctcagcg atcgcgtgac agccgaccga ttcagccccg agggacttcg cactgcgctg
     3721 gaaaagcggc tcgaagggct cgccggcagc gccggcgcgt tcgaaaagac attgctggcg
     3781 tccgatccta caggcgagac attgcatgtg ctgcagcgac tgacgcctga caggcaaccg
     3841 cgccgcatcg atgacgcctg gttcgacgag acagggagga gtgcgctgct ggttgtcgag
     3901 acccgtgcgg cgggctccga cctcgccggg caggacgcgg ccctcaagag gcttggcgct
     3961 gcgtttgccc tcgttcgcgg cgacggcgct gcccggatgc agtattccag tccgggcgcg
     4021 atggctgtcg agagcagggc gctgatcacc gccgatgtca catggctgtc gctgctttcg
     4081 acggttctca tcctcttgat cctggggtcg gtctatcgct cgctccttgt cgtgctggtc
     4141 tgcgtcctgc ctgcgctgag cgggctcctc gcgggcattg tcgcggtcaa tttctggttc
     4201 gggaccgtgc acgccattgc gctcggcttc ggggcgactc tgctcggcga ggcagtggac
     4261 tacccgagtt accttttgac gcaaaggcgt cccggcgaag cgatgcgcga cacgcttgcg
     4321 cggatgctgc cgatgctgcg cctggcggta gcgacgacga ccttcggggc actggcgctc
     4381 ctgatgtcgc atttttccgg gctggcgcaa ctcggcctgc tgaccgtcgt gggcgtcgtc
     4441 gtcgccggcc tggtcaccgc gctcgtgttg ccgcatctga taccgccggg atggcatccg
     4501 cgattggcga cggcacgacc ggcgaggctc ccggacgcat tcgccggaat cgggccgcgt
     4561 gccgccatcg tgctgatcat cgcggcaact gtcatggcgc tggccgcgaa ccgaccgtgg
     4621 tgggacgacg acctggccca tatgaatccg ttgccgccgg cggtgctggc acaggatcga
     4681 caattgcggc aagccctgaa tgcgccagag gtcggttcgc tgctcgtcgc cagtggcgcg
     4741 acgcgcgagg aggtgctcgg aatagcggag tcgcttcgac cggcgctcga ggcctggcag
     4801 gcggccggcg acatcggcag ctttacgctg gtgagcgatt acctgcctag cgcgacaacc
     4861 cagacgatgc ggcgggcggc gctgcccgag gcaccgcgtc tgcgctcgaa tctcgatgcg
     4921 gcgctcgcag ggctgccttt tcgcgccgac acgttcgagc cgttcctgcg ggacgtcgag
     4981 caagcacggg cggccaagcc gttgacgtcc gaagcgttgc agggcagcgc gctcggactg
     5041 aaagtggacg cgctgctcag cgaggatggt ggccgctggt ctgccgtggt gccgatgacg
     5101 cgcgtcacca gccccggtat gatgtccggc aaggttgcgg cgctacgcgt gcccggcgcg
     5161 caatggatcg acttgcgcac gcaggcggtg gcgatggtaa ccgcctatcg ctggcagacg
     5221 ctgacgtaca ccgcgatggg catcctgatg atatacggcg ctcttgccta cggattgaag
     5281 agttggcgtc gagcgatcga ggtgctgttc cccgtagtca tcgcgctgct cctgacgacg
     5341 gggacgctgg tagcttccgg ccaaccagtt tccattttcc atctggtcgc gctgctgctg
     5401 acgctgggca tcggtaccaa ctatgcgctt ttccttgcca gagcgagcca gccgggcgct
     5461 gagcgcagcg gcacgctgcg cacgctggca gtcgtcggcg gcacaacgct ctgcgcgttc
     5521 ggtgcattga cgctatcgag cacgccggtc ctgcacgcga ttggcatgac cgtgtgtctg
     5581 ggtgtcctct acagtctgct gttgagcctg ctgctgcttt ggccaccgcc gttgccatcg
     5641 acattgccat cgacatcaac ctcgccgatc gacgaatgac gccgctgccg ctggcggcgg
     5701 ttacgctgac caacgcctgt ggcgtcggcc tggacgagat acggcgcgcg ttgcgcacag
     5761 gcagcagcgg tctccggcaa aacgatttcg agccggcgcg cgggctcgac acgtggatcg
     5821 gccgcgtcga cggtctggag tcggaagcgc ttgcgccgga ctgcgtggcg cacgattgtc
     5881 gcaacaaccg tctggcgcgg ctatctctcg accaggacgg ctttgtcgcg gcggtcgacg
     5941 cggcgcgttc gcgctatggc gagggccgcg taggcgtgtt tatcggcacg acgacttcgg
     6001 gaattctgtc gaccgagctc gcgtatcggt cgggatcgct ggcgtccggc ggcccatcgc
     6061 cgccggagga cgagccgtcg gcaggcttgt cgccggagct ttatcgctac aagcacagca
     6121 tgtacgcgac gacgatgttt gttcgctcct acttaagtct ttccgggtac gcgatgacga
     6181 tctcgacggc gtgctcgtcc agcgccaagg ttttcgccgc tgctgcccgg gcgatagacg
     6241 ctggactgtg tgatgctgcg gtcgtgggtg gtgtggattc cctggccttg tcgacgctgt
     6301 tcgggttcca ttcgctggag ttgctgtcgc gccggccttg tcgaccgttc gcatcggacc
     6361 gcgacggaat ttcgatcggc gagggtgccg gcttcgcgct gctcgacccg caagccgaga
     6421 gcccggtgcg ccttctcggt gccggcgaga gtgccgacgc ctatcacatg tcgagtccgc
     6481 atccggaggg cgcgggcgcg gccgccgcga tgcgcaaggc gctttccggt gcggggttag
     6541 tggggcgcga catcgattac atcaatctgc acggcacggc cagccgtgcc aacgatttgg
     6601 ccgaggatcg gggagtggtg tcggcggtcg gcaaagagcc ggcggcgagc tcgaccaagg
     6661 gatggaccgg acacttgctc ggcgcggccg gcatcaccga ggcggtgatc agcgccctct
     6721 ccctcatcga tcaatggata cccggtacgc tcaactgcga gcgcgtcgat ccggcgctgc
     6781 acagcaacct tctcgtcgat tcgcagacta tgcgtttgcg tcacgtgatg agcaattcgt
     6841 tcggtttcgg cggcagcaat tgcagcctgg tgttcggact cggccaatga agcctctggc
     6901 gatcaccggc gtcggcgtct gcggcgccgg cttgcacgac tggacgcagg cgaagcctgt
     6961 gctggatgga acggctcgat tcgaagctcg ggcagtggac aagttcgatc tcgcctgcct
     7021 gccaccgatc gagaggcggc gcgcgaacga gagttcgcgt ctggcgatgg ttgcggcgct
     7081 ccaggccatc gagcatgtga gtattgcaga gcgagagcgg ctgtcgacgg tattcagttc
     7141 ggccgacggc gataccgaag tactggcgca cctgctcgcc aagctggcga ctagcgacat
     7201 catgctttcg ccgacgttgt ttcacaactc agtgttcaac gcgccggccg gatactggag
     7261 ccttggctgc aaggcacagg cggcgtcgac gacggtgtgt gccgggcgag cgtcgtttgc
     7321 ggtggggctg tgcgaagtga actcgcaagt actggccagc ggagaggcgg tgctgtatat
     7381 cgcctacgac atggcgtttc ccgaggcact gaagcgtctc ggccggacga cgatggcatt
     7441 tgcctgtgcg ctgctgctgg agccgacgag cgacggcgcg cctgggcgct tcggtatgat
     7501 cgaggggtgg caacgcgaag tcggagcgcg tgagactgac gctgggttgc aggcgttggc
     7561 ggaagggttc gccggcaacg ccgccgcggc agcgttgcca ttgctgacgg cgatagcgac
     7621 gcaggccggc accgagattc gcctgcctta cctcgacgac agccggctgc acttgcggta
     7681 ttccccatgg tcctgaacca ggacgccatc cgcgggctga tcccgcaccg agcgtcgatg
     7741 tgcctgctcg atacggtgga gtattgggat gccgaacgtg tagtgtgtcg aaccagtcag
     7801 catcgttcgc cgggcaatcc gctgcgtgcc aacgcgcgat tgtcgagtat ccacggcatt
     7861 gaattcgcgg cccaggcgat ggccgtgcac ggcggactgc tggcgccgaa cggcgcggcg
     7921 cctagcgtcg gactccttgt cagcttgcgc gattgcgtgc ttcactgcga gcgtctggac
     7981 gtcctggatt caccattgac ggtcgaggcg cgtcgaatca cgggcaacaa cggcctcacg
     8041 atgtacggat tctcggtgac tgcgaacacg ctggtaatcg aaggtcgcgc cagcgtgctg
     8101 ctgcaacgcg aggctgcggg atgaagcggg cattggtcac cggcggaagc ggcagcatcg
     8161 gcgcggcagt ctgccgcacc ctggcgtcgg ctggtcacca cgtattggtg cacacccaca
     8221 gccggcccga caaagcggcg gcgatcgtca ccgccatcac cgaggcaggc ggttcggccg
     8281 acgccgttca attcgatgtc gccgacggtg acgccacagc tctggtcct
//
LOCUS       DATANS010000324         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_311657, whole genome shotgun sequence.
ACCESSION   DATANS010000324 DATANS010000000
VERSION     DATANS010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311657"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VHT22_04685"
     CDS             complement(<1..644)
                     /locus_tag="VHT22_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEX3136881.1"
                     /translation="MKFKVKRVHFVGIGGAGMSGIAEVLLTQGYEVSGSDVALSATTR
                     RLSELGAKIAAGHDERNVAGANVVVVSTAVAADNPEVVAARERGVPVVPRALMLAELM
                     RLKQGIAVAGTHGKTTTTSLIASVLAEGGLDPTFVIGGRLLSAGVNARLGKGDFLVAE
                     ADESDASFLVLQPVVAVVTNIDADHMATYGHDFARLKDAFVNFVQRLPFYGVAVL"
     gene            complement(641..>1590)
                     /gene="murG"
                     /locus_tag="VHT22_04690"
     CDS             complement(641..>1590)
                     /gene="murG"
                     /locus_tag="VHT22_04690"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX3136882.1"
                     /translation="PQHGIDYEPIRFAGVRGKGFKRLLLGPFALLSAFWQSLGIIRRR
                     APDVVLGLGGYVSFPGALMAVACNRPLVIHDANAVAGLANRILAYGADRILLGFPGAL
                     GNRHARKVEWVGNPLRAEIVAVPPPEQRFAGRHGTMSLLVVGGSLGATALNERVPAAL
                     ALLDRESRPHVVHQAGERHIDALRRAYEAAGVEAVCLPFIDDIARQYADADLVICRGG
                     ALTISELAAVGVGSVIVPLPGAFANEQSHNGQFLVDAGAAVMLKQSAMTAQGLADLLL
                     AMNRGRMLAMAIAARRVGRTDASERVADACIALAQRSAA"
BASE COUNT          263 a          563 c          524 g          240 t
ORIGIN      
        1 agcaccgcga cgccgtagaa cggcaggcgc tgcacgaaat tcacgaatgc atccttgagg
       61 cgcgcgaagt cgtggccgta ggtagccatg tgatcggcat caatattggt cacgacggca
      121 acgaccggtt gcagcacaag gaatgacgcg tcggactcgt cggcctccgc caccaggaag
      181 tcgcctttgc ccaggcgcgc gttgacaccc gccgacagca accgaccgcc gatgacaaag
      241 gttggatcga gtccgccctc ggccagcaca ctggcgatca ggctggtcgt cgtcgtcttg
      301 ccatgtgtgc ccgcgacggc gatcccctgc ttcaaccgca tcagctcggc cagcatcagc
      361 gcacgcggca caacaggcac gccgcgctcg cgcgcggcga cgacttccgg attgtcggcg
      421 gcgaccgctg tcgatacgac gaccacgttg gcgcccgcga cgttgcgctc gtcgtgtccg
      481 gccgcgatct ttgcgccgag ctcgctcaag cgccgcgtcg tcgccgagag cgcgacgtcc
      541 gagccgctga cctcgtaacc ctgtgtcagc agcacttccg cgattccgct catgccggcg
      601 ccgccgatgc cgacgaagtg cacgcgcttg accttgaatt tcatgcggcc gacctttgcg
      661 ccagcgcaat gcaggcatcg gcaacccgct cgctggcgtc ggtgcggccg acccgtcgcg
      721 cggcgatcgc catcgccagc attcggccgc gattcatcgc aagcagcagg tcggccaggc
      781 cctgtgcggt catcgccgac tgcttcagca tgaccgccgc gcccgcgtcg accaggaatt
      841 gcccgttgtg cgattgctcg ttggcgaatg ccccgggcag cggcacgatc acgctgccga
      901 cgccgacggc ggcgagctcg gagatcgtca atgcgccgcc gcggcagatc accagatccg
      961 cgtcggcgta ctggcgtgcg atgtcgtcga tgaaaggcag gcagaccgcc tcgacgccgg
     1021 ccgcttcgta ggcacgccgc agcgcgtcga tgtggcgctc gcctgcctgg tgcaccacgt
     1081 gcggccgcga ttcgcgatcg agcagcgcaa gcgccgccgg cacgcgctcg ttcaacgcgg
     1141 tggcgcccag gctgccgccg acgaccagca agctcatcgt tccgtgtctt ccggcaaagc
     1201 gctgctcggg cggcgggacg gcgacgatct ccgcgcgcag cggattgcct acccattcca
     1261 ccttgcgcgc gtgcctgttg ccgagggcgc cgggaaatcc gagcaggatg cggtcggcgc
     1321 cgtacgcgag gatgcgattg gcaagccccg cgaccgcgtt cgcgtcgtgg atcaccagcg
     1381 gccggttgca cgcgaccgcc atcagcgctc ccggaaacga gacgtagccg ccgagtccca
     1441 gcacgacgtc cggcgcccga cggcggatga tgccgaggct ttgccagaaa gccgacagca
     1501 gcgcgaaagg cccgagcagc aggcgtttga atcccttgcc gcgcacaccg gcgaagcgga
     1561 tcggctcgta gtcgatgccg tgctggggga
//
LOCUS       DATANS010000325         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_100947, whole genome shotgun sequence.
ACCESSION   DATANS010000325 DATANS010000000
VERSION     DATANS010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100947"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VHT22_04695"
     CDS             <1..705
                     /locus_tag="VHT22_04695"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX3136883.1"
                     /translation="VGDFDVAAVKDQVQRLFGAWSAPSRYARVPDPLVTKRAAAMPIE
                     TPDKANAFLIGQTAFALSDTDPEYPALMLANYMLGGSTNARLWNRVRQKEGLSYGINS
                     SMHASSWEPNTTISISAIFAPENVERLRSAVQDEVERAQREGFSAEEVADAKRALLQQ
                     RRLARTQDATLAAALNEQSFVGRTFNYSATLDAALERLDAPAVNAALRKYLKPESFAS
                     VFAGDFAKVKKPQPPG"
     gene            complement(730..>1581)
                     /locus_tag="VHT22_04700"
     CDS             complement(730..>1581)
                     /locus_tag="VHT22_04700"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX3136884.1"
                     /translation="KRAPFRPMTAQHGAPRKDFMPLTARYAAAQQVALRAGELARDLF
                     AKRDSLIVEHKGAQNFVSRADRAVEDFIASKLKAAFPNDAFLGEESAASFAGTPEHVW
                     VVDPIDGTHNFLRGLRYYCVSIAYVEVGQTEIGVVYDPEHDEMFHARRGEGAWRRHGG
                     DQGKIAVSDCAALAKALLAIGHHDRFVEPRYLALRHALMDAGAATRNLGAGALQLAHV
                     AAGRLDGFVELSLNAWDALAGLLLVEEAGGYVAPFPGPERWREPAPVLGCAPGIAGAL
                     QPLVGAW"
BASE COUNT          274 a          545 c          526 g          236 t
ORIGIN      
        1 gtcggcgatt tcgacgtcgc tgccgtaaag gaccaggtgc agcgcctgtt cggcgcatgg
       61 tcggcgccga gccgctacgc gcgcgtaccg gacccgctgg tgaccaagcg cgcggcggcg
      121 atgccgatcg aaacgccgga caaggcgaac gccttcctga tcgggcagac ggcgttcgcg
      181 ctcagcgaca ccgatcccga gtacccggcg ttgatgctcg ccaattacat gctcggcggc
      241 tcgaccaatg cgcggctgtg gaatcgcgtg cgccagaaag agggcttgag ctacggcatc
      301 aacagctcga tgcatgccag cagctgggag ccgaatacca cgatctcgat ctcggcgata
      361 ttcgcgcctg agaatgtcga gcgcctgcgc agcgccgtgc aggacgaggt cgagcgcgcg
      421 caacgcgagg gcttcagcgc cgaagaagtg gccgacgcca agcgcgcgtt gctgcagcag
      481 cgacgccttg ctcgaaccca ggatgcgacg ctcgccgcgg ccttgaacga acagtcgttc
      541 gtcggccgca ccttcaacta ctcggccacg ctcgatgccg cactcgaaag gctcgatgcc
      601 ccggcggtca atgcggcgct gcgcaaatac ctgaagccgg aatcgtttgc cagcgtcttt
      661 gccggcgatt tcgcgaaggt gaaaaaaccc cagccgcccg gttgacgctg cgccgccgct
      721 gagcctcgcc taccaggcgc cgaccagcgg ctgtagcgcg ccggcgatgc cgggcgcgca
      781 gcccagcacg ggcgcaggct cgcgccagcg ctccggcccc gggaacgggg cgacgtagcc
      841 gccagcttcc tcgaccagca agaggccggc gagcgcatcc cacgcattca gggacagctc
      901 gacaaagccg tcgagccggc cggcggcgac atgcgccagc tgcagggcgc ccgcgcccag
      961 attgcgcgtc gccgccccgg catccatcag cgcgtgacgc agcgccagat agcgcggctc
     1021 gacgaaacgg tcgtggtgtc cgatcgcaag cagcgccttg gcgagcgccg cgcaatcgct
     1081 gaccgcgatc ttcccctggt cgccgccgtg gcggcgccat gcgccctcgc ctcgccgcgc
     1141 gtggaacatc tcgtcgtgct cgggatcgta gacgacgccg atttccgtct ggccgacttc
     1201 gacgtatgcg atcgacacgc agtagtagcg caagccgcgc aggaaattgt gtgtgccgtc
     1261 gatcggatcg acgacccaga cgtgctcggg tgtgccggca aacgatgccg ccgattcctc
     1321 gccgaggaag gcatcgttcg gaaacgccgc tttgagcttg ctggcgatga aatcctcgac
     1381 cgcgcgatcg gcgcgactga cgaagttctg cgcgcccttg tgctcgacga tcagcgaatc
     1441 gcgcttggcg aacaggtcgc gagcgagctc gccggccctg agcgcaacct gctgcgcggc
     1501 cgcatagcgg gcggtcaacg gcatgaaatc ctttctcggc gcgccatgtt gcgcggtcat
     1561 cggccggaac ggcgcgcgct t
//
LOCUS       DATANS010000326         2077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_478635, whole genome shotgun sequence.
ACCESSION   DATANS010000326 DATANS010000000
VERSION     DATANS010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478635"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..721)
                     /locus_tag="VHT22_04705"
     CDS             complement(26..721)
                     /locus_tag="VHT22_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136885.1"
                     /translation="MRLFSCVLVRIAPLVLLAACATVPVGPPIASPRFVVGDHWQYRV
                     IDNMRRGAVAQIDTEVVALAGDVATLRVVKVNDGVRSEWTDEIEGGGGLRAGMLDGST
                     RRFAPAAALLAFPLEQGKTWRQVVPTFRRDIELPDQILIYGDVQGRAAVTVPAGSFDT
                     VRLYRVLDLDDRQPWRTRTTRRDQVWYAPEVKGVVREAREATYAEPGDSGATQVYTER
                     TVTELVSFRPGRG"
     gene            complement(972..>2077)
                     /locus_tag="VHT22_04710"
     CDS             complement(972..>2077)
                     /locus_tag="VHT22_04710"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX3136886.1"
                     /translation="GACGGQYTANTMSSSFEALGMSLLGSSQMASPDPEKADSAAASA
                     GALVHAIDLDLKPRDIITRRSIENAIALVMATGGSTNAVLHYLAIAAAAGVEWSIDDF
                     ERVRQRVPVLCDLKPSGRFVATDFHAAGGVPQVLKMLLAHDLLDGDCMTITGKTIGEE
                     LSDVPDTPRRDQEVIRPWDRPVYTQGHLAILKGNLAPEGCVAKITGLKSLSITGPARV
                     FDSEPAALDAIIGKKIKPGDVMVIRYEGPKGGPGMQEMLAPTSALIGQGLGDSVGLIT
                     DGRFSGGTWGMVVGHVAPEAYVGGMIALVEEGDSITIDAHRRLIQLNVDDAKIAARRA
                     KWSPPPPRYTRGVLAKYMKLVSTASRGALTDAG"
BASE COUNT          343 a          716 c          669 g          349 t
ORIGIN      
        1 tagaagttcg gcagacgccg gacagctagc cgcgaccagg ccggaacgag acgagctcgg
       61 tgaccgtgcg ttcggtatag acctgcgtcg cgccgctgtc cccgggttcg gcgtaggtgg
      121 cctcgcgcgc ctcgcgcacc acccccttga cctcgggcgc ataccagacc tgatcgcggc
      181 gagtcgtccg tgtgcgccat ggctgccggt cgtcgagatc cagcaccctg tacagccgga
      241 cggtgtcgaa gctgcctgcc ggcacggtca cggccgcgcg cccctgcacg tcgccgtaaa
      301 tgaggatctg gtcgggcagc tcgatatcgc ggcgaaacgt cgggacgacc tggcgccagg
      361 tcttgccctg ctcgagcggg aacgcgagca gcgcggcggc cggcgcgaag cggcgcgtcg
      421 agccatccag catgcctgcg cgcaggcctc cgccgccttc gatttcatcg gtccattcgg
      481 aacgcactcc gtcattgacc ttcacgacgc gcagcgtcgc gacatctccg gccagcgcta
      541 cgacctcggt gtcgatctgc gccacggcgc cgcgccgcat gttgtcgatc acgcggtatt
      601 gccaatggtc gccgacgacg aatcgcggcg atgcaatcgg cggaccgacc ggcaccgtcg
      661 cgcaggcggc gagcagcacc agcggggcaa tccgcaccaa gacgcaggag aacaggcgca
      721 tcgacgtctc ctcctcgcgc agtcgcgagc cttaaggggc ggctgaccgc cgacgcgatt
      781 cagccggcgt gcccgccggc gttcaggccg agtacgcccg cgacgatgag cacggtactt
      841 acgatcttca gcgtcgtcat tggttcctgg aagcagcgaa gccgatccga gcgatcaggg
      901 ccgttcctgc gcccgaccct atcgcacaag aaacgctgat gtcgattttg ttgcgaaccg
      961 gcggttgtga cctagccggc gtcggtcagc gctccgcggc tcgcggtcga taccagcttc
     1021 atgtacttgg ccagcacgcc tcgcgtgtag cgcggcggtg gcggcgacca tttcgcgcga
     1081 cgcgcggcga tcttggcgtc gtcgacgttg agctggatca gccgccggtg cgcatcgatc
     1141 gtgatcgagt cgccctcctc gaccagcgcg atcatgccgc cgacgtacgc ctcaggcgcg
     1201 acatgaccga cgaccatgcc ccaggtgcca cccgaaaagc ggccgtcggt gatcaatccg
     1261 acgctgtcgc ccaagccctg gccgatcagt gccgatgtcg gcgccagcat ttcctgcatc
     1321 cccggaccgc ctttcgggcc ttcgtagcgg atcaccatca cgtcgcccgg cttgattttc
     1381 ttgccgatga tcgcgtccag cgctgccggc tcggaatcga acacccgcgc cgggccggta
     1441 atagacagac tcttcaagcc ggtgatcttg gccacgcagc cctccggcgc gagattgccc
     1501 ttgaggatcg ccaaatggcc ctgcgtgtac accggccggt cccagggtcg gatcacctcc
     1561 tgatcgcggc gcggcgtgtc ggggacgtcg gagagttcct cgccgatcgt cttgccggta
     1621 atcgtcatgc agtcaccatc gagcagatcg tgggcgagca gcattttgag cacctgcggc
     1681 acgccgcccg ccgcatgaaa gtcggtggcg acaaagcgtc cggagggttt gagatcgcag
     1741 agcacgggca cgcgctggcg cacgcgctcg aaatcgtcga tcgaccactc gaccccggct
     1801 gcggcggcga tcgcgaggta atgcagtacc gcgttggtcg agccgccggt cgccatcacc
     1861 agcgctatcg cgttttcgat cgagcggcgt gtgatgatgt cgcgaggctt caaatccaga
     1921 tcgatcgcat gaaccagcgc gcctgccgac gccgccgcgc tatcggcctt ttcgggatca
     1981 ggcgacgcca tctgcgagct gccgagcaga ctcatcccca gtgcctcgaa cgacgaggac
     2041 atcgtgttgg ctgtgtattg cccgccgcag gcgccga
//
LOCUS       DATANS010000327         3446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_311746, whole genome shotgun sequence.
ACCESSION   DATANS010000327 DATANS010000000
VERSION     DATANS010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311746"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(146..389)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            479..1855
                     /gene="miaB"
                     /locus_tag="VHT22_04715"
     CDS             479..1855
                     /gene="miaB"
                     /locus_tag="VHT22_04715"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX3136887.1"
                     /translation="MSRKLYIRTFGCQMNEYDSAKMADVLHAAEGLEPTARPDDADVI
                     LFNTCSVREKAQERVFHDLGRVRALKTANPELIIGVGGCVASQEGAAIVARAPYVDVV
                     FGPQTLHRLPELIARRRATGQPQIDISFPEIEKFDHLPPPRVDGVAAFVSIMEGCSKY
                     CSFCVVPYTRGEEVSRPFDDVLTEVADLVDQGVREVTLLGQNVNAYRGSAGEGEIADL
                     TALIEHLAEIDGLERIRFTTSHPKEMSQRLIDAYRTVPKLVSQLHLPVQSGSDRVLAA
                     MKRGYSALEYKSIVRRLRKARPALSLSSDFIVGFPGETDADFEQTMRLIDEVGFDGSY
                     SFLYSPRPGTPAANFGDAVPREIGQARLARLQQRIDTQYAACSAAMVGTIERALVTGS
                     ASRDSAELSARTECNRVVNFAGSADLIGHYADIRITAARAHSLRGELAVGQGALRPEQ
                     PACLPFNP"
     gene            1874..>3446
                     /locus_tag="VHT22_04720"
     CDS             1874..>3446
                     /locus_tag="VHT22_04720"
                     /inference="COORDINATES: protein motif:HMM:NF028457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5117 domain-containing protein"
                     /protein_id="HEX3136888.1"
                     /translation="MTAQFSPVSRLTIALAAASLLAGGCASVPATDSATLAAAAVAAT
                     GAARAATENTSPIATGAGHAPTEPGSAKAGVPAVAAAAAAAAAAAVAAQQKPFAEVVK
                     DAKEQPGFFNVYSKDEKVWLEIQPDQIDRPFFLQANRTQGIGERDPFPSPMLRSYIVE
                     FHRVGNLVQLIAKNTQFIAVAGSPLARAVRESTSDSLLGSVPVASQPHPERKSVLIEL
                     NPLLLSDLPAGSSALESAYRIPYAFDARNSSFTGIHNGADLTGFGVTAHYAVSKLPSR
                     PTTPSAAASRVPPPAHLEDARSLFLGYYYSLARLPEPMHARAADDRLGHFVARRWDFS
                     DDIKTFPEVYYVKRWRLEKKDPEAALSEPKQPIVYWLDRDIPEKYRDTVKAGILEWNK
                     AFEKIGFKDALRVELQPENADFDTADARHASVRWVVRDEPGALAIGPSRADPRTGEIL
                     DADIEIEDGWTRLPRRQASEQFHPRTQSLPTGEDTFCEYGDIAMDELAFALDVLVARG
                     DIDPNGPEAQKYVLAT"
BASE COUNT          602 a         1188 c         1087 g          569 t
ORIGIN      
        1 tctccgcaat gccgtgcgtc cccgcacggc ggttggtggc aggcaacgcg gagaaaacgt
       61 tcgggcgaag gccccggcga caagcggcat ccggccggac atcgcctccc cgcgatgcgt
      121 ctcggtccga aagccatgcg cgaacgtcag gcattccggg gcaggccggt ctccgggctg
      181 gtcggtatgc gaaaggctca acgccttagc gccggacggt ccgccttccc atgcgcgacg
      241 cgcacagtgg catttcgagg accgcccgca ccgacttacc gttgcggggg cagcgccggc
      301 tttgccgcat gaacatgcgg cgcaccggct tcccgtttca cccgcggcgc caaactggcg
      361 cggcggacac ctgctgcggc cgagtatacc gctccgcggt gacagacggc ttgcgcttcc
      421 cgggactgct atactcttcg gcccttcgta tccagcccaa gacgcccgac cgctatccgt
      481 gagccgcaag ctgtacattc gcacgtttgg ctgtcagatg aatgagtacg actcggccaa
      541 gatggccgac gtgcttcacg cggccgaggg cctcgagccg accgcccgac cggacgacgc
      601 cgacgtcatc ctgttcaaca cctgctcggt gcgcgagaaa gcgcaggagc gcgtgttcca
      661 tgacttgggg cgcgtgcgcg cgctgaaaac tgcgaatccg gagctgatca tcggcgtcgg
      721 cggctgcgtc gccagccagg aaggtgccgc gatcgtcgcg cgcgcaccgt acgtcgacgt
      781 ggtctttggc ccgcagacct tgcatcgcct gcccgagcta atcgcacgcc ggcgtgcgac
      841 cggccagccg cagatcgaca tcagctttcc cgagatcgag aagttcgacc acttgccgcc
      901 gccccgcgtc gacggcgtcg cggcattcgt gtcgatcatg gagggatgca gcaagtactg
      961 ctcgttctgc gtcgttccct acacccgcgg cgaggaagtg tcgcggccgt tcgacgacgt
     1021 gctgaccgag gtcgccgacc tggtcgacca gggtgtcagg gaggtcaccc tgctcggaca
     1081 gaacgtcaac gcgtaccgcg gcagcgcagg cgagggcgaa atcgccgatc tgaccgcact
     1141 catcgagcac ctcgccgaga tcgacggcct ggagcgcatc cgctttacga cctcgcaccc
     1201 gaaggaaatg tcgcagcggc tgatcgatgc ttaccgcacg gtaccgaagc tcgtctcgca
     1261 gcttcatctg ccggtgcaat ccggatccga tcgcgtgctg gcggcgatga agcgcggcta
     1321 cagtgcgctc gagtacaagt cgatcgtccg ccgcctgcgc aaggcccgtc cagcgctgtc
     1381 gctgtcatcc gatttcatcg tcggatttcc cggcgagacc gatgccgact tcgagcagac
     1441 gatgcggctg atcgacgagg tcggattcga tggcagctac agcttcctgt acagcccgag
     1501 acccggcacg ccggcggcca atttcggcga cgccgtgccg cgcgagatcg gacaggcgag
     1561 gctcgcgaga ctgcagcaac ggatcgatac gcaatacgcg gcttgcagcg cggcgatggt
     1621 ggggaccatc gagcgcgcgc tggtcaccgg cagcgccagc agggattcag cggagctctc
     1681 ggcgcggacc gagtgcaatc gtgtcgtcaa tttcgccggc agcgccgatc tgatcggaca
     1741 ctatgccgac atccgcatta ccgcggcgcg cgcacattcg ctgcgcggcg agcttgccgt
     1801 cggtcaaggc gcgctacgcc cggaacaacc ggcctgttta cccttcaatc cttgagaatt
     1861 acctgaagcc accatgaccg cacaattctc cccggtttcg agacttacga tcgcactcgc
     1921 cgccgcgtcg ctgctggccg gcggctgcgc cagcgtccct gcaaccgatt cggcgacgct
     1981 ggctgccgcc gcagtcgccg cgaccggcgc cgcgcgcgcc gcgaccgaaa atacctcccc
     2041 gatcgccacc ggcgccggtc atgctccgac cgagcccgga agcgcgaagg cgggtgtgcc
     2101 ggccgttgcc gccgccgctg cggctgcggc agccgctgcc gtcgccgctc agcagaaacc
     2161 gttcgccgaa gtcgtcaagg atgccaagga gcaaccgggc tttttcaacg tctacagcaa
     2221 ggacgaaaag gtctggctgg aaatccagcc ggaccagatc gatcggccgt tcttcctcca
     2281 ggcgaaccgc acgcagggca tcggcgagcg cgacccgttt cccagcccga tgctgcgttc
     2341 gtacatcgtc gaattccacc gggtgggcaa tctggtgcag ctgatcgcga agaacaccca
     2401 gttcatcgcg gttgccggct caccgcttgc acgcgcagtg cgcgaatcga ccagcgacag
     2461 cctgctgggc tcggtgcccg tcgccagcca gccgcacccg gagcggaagt cggtcctgat
     2521 cgaactcaat ccgttgctgc tgagcgatct tccggccggg tcgagcgcgc tcgagtcagc
     2581 gtaccgcatt ccctacgctt tcgatgctcg caattcgagc ttcaccggca tccacaacgg
     2641 cgccgacctc accggtttcg gcgtgaccgc gcattacgcg gtatcgaaac tgccttcgcg
     2701 gccgacgact ccctcggccg cggcttcgcg cgtaccgccg ccggcgcacc tcgaggacgc
     2761 gcgcagtctc ttcctcggct actactacag cttggccagg ctgcccgagc cgatgcacgc
     2821 ccgcgcggcc gacgatcgcc tcgggcattt cgttgcgcgc cgctgggatt tttccgacga
     2881 catcaagact ttcccggaag tctattacgt caagcgctgg cgtctggaga aaaaggatcc
     2941 tgaggccgcg ctgtccgagc cgaaacagcc tatcgtctac tggctcgacc gcgacatccc
     3001 ggaaaaatac cgtgacacgg tcaaggccgg gatcctcgaa tggaacaagg cgttcgaaaa
     3061 gatcggattc aaggatgcgc ttcgggtcga gcttcagccc gagaacgccg atttcgacac
     3121 cgccgatgcg cgccatgcct cggtgcgctg ggtcgtgcgc gatgaacccg gcgcgctggc
     3181 gatcggtccc agccgcgccg atccgcgtac cggcgagatt ctggacgccg atatcgagat
     3241 cgaggacggc tggacccgct tgccgcggcg ccaggcttcc gagcaattcc atccgcgaac
     3301 ccaatcgctg cccactggcg aagacacgtt ttgcgaatac ggcgacatcg cgatggacga
     3361 gctcgcgttc gcgctcgacg tgctggtcgc gcgcggtgac atcgatccga acgggccgga
     3421 agcgcagaag tacgtgctgg cgacgc
//
LOCUS       DATANS010000328         3713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_100989, whole genome shotgun sequence.
ACCESSION   DATANS010000328 DATANS010000000
VERSION     DATANS010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100989"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /gene="htpX"
                     /locus_tag="VHT22_04725"
     CDS             <1..831
                     /gene="htpX"
                     /locus_tag="VHT22_04725"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEX3136889.1"
                     /translation="VLSVVVRLLGLDRALAANGLDVGALLVFSAVVGFTGAIISLLMS
                     KPMAKWSTGAQVIETPKNENEAWLVATVKRLAGKAGVGMPEVSIYEGEPNAFATGAFR
                     NSALVAVSTGLLSSMSREEIEAVLGHELSHVANGDMVTLTLIQGVVNTFVVFLSRVVA
                     FFVDRIVFRTERGNGPGYFITMIVAQIVLGVLASMIVAWFSRRREFRADAGSARLLGT
                     PQPMMHALARLGGLPPGELPKSMTGFGITDHSGFMALFATHPPIEERIRALQAGAAAR
                     "
     gene            853..1095
                     /locus_tag="VHT22_04730"
     CDS             853..1095
                     /locus_tag="VHT22_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136890.1"
                     /translation="MAAPVRYKIEIEAANGIWSDVRGGDGAVLTFDSEAEARTRLAEL
                     YPVETQMERYGGGKRTRVIRILPDDEDDWPKRGAPR"
     gene            complement(1218..1622)
                     /locus_tag="VHT22_04735"
     CDS             complement(1218..1622)
                     /locus_tag="VHT22_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEX3136891.1"
                     /translation="MATAKKAKKPAKKPAAKKPAAKKSAAKKPAAKKAPAKKAAPKKA
                     AKKPAAKRKPNAAFMKAMTPSSTLAAVVGSNPLPRTEVTKKIWDYIKKNKLQDSINRR
                     LINADDKLRQVFGGKRQVSMFEMTKLVSNHLK"
     gene            1976..2629
                     /gene="pdxH"
                     /locus_tag="VHT22_04740"
     CDS             1976..2629
                     /gene="pdxH"
                     /locus_tag="VHT22_04740"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX3136892.1"
                     /translation="MTPSQNLAELRSEYHRQALTEADVDPDPLRQLERWLQEAIRAAL
                     PEPTAMTLATVGADDRPAARIVLLKGVGPDSLVFFTNYESRKGRELAIHPDAALLFHW
                     IELERQVRIEGTASKVAGAESDAYFASRPRSSRLGAWASPQSRTIPDRDWLEREFAAV
                     AVRYEGDNAAVPRPPFWGGYRVVPSLVEFWQGRPSRLHDRIVYRRDGPRWQVERLAP"
     gene            2626..>3713
                     /locus_tag="VHT22_04745"
     CDS             2626..>3713
                     /locus_tag="VHT22_04745"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3136893.1"
                     /translation="MTPPAGRSRYSFPVLIALAIVNHSVLAGSRVTVSLYALSIGASP
                     LVVGTLMGLYAFLPIWLSVAAGRLSDRVGVRGPMLVGSFGVALGAALPCIWPGIGALF
                     AATSLIGASFMLFQVSVQNATGEMGPPGERAKNFSLLALGYSISGFCGPLAAGLSIDH
                     LGFGTTFIGFALLPLLPVLVLTRRALVLPGRHRAHAQAPSGGIMQLMRNPQLKRVFIV
                     NALLSMAWDLHTFFIPIYGATIGLSASRIGVILASFAAATFAVRLMMPRIARSFTEFQ
                     VLTAAMFIAAATYALFPFVASAGALMVLSFALGLALGSAQPMVMALLHSIAPAGRMGE
                     AAGVRMSIINASTFAVPLLFGAVGSTLG"
BASE COUNT          525 a         1194 c         1244 g          750 t
ORIGIN      
        1 gtgctgtcgg tcgtcgtgcg cttgctgggt ctcgaccgcg cgcttgccgc gaacggcctc
       61 gacgtcggcg ccttgctcgt cttttcggcg gttgtcggct tcaccggcgc gatcatttcg
      121 ctgttgatgt cgaagccgat ggccaagtgg tcgaccggcg cccaggtgat cgagacgccg
      181 aagaacgaga acgaggcctg gctggtggcg acggtcaagc ggctggccgg caaggccggc
      241 gttggcatgc ccgaggtctc gatctacgag ggcgagccga acgcgtttgc gaccggcgcg
      301 ttccgcaatt cggcgctggt cgcggtgtcg accggattgc tgagttcgat gtcgcgcgag
      361 gaaatcgagg cggtgctcgg ccacgagctt tcgcacgtcg ccaacgggga catggtgacg
      421 ctgacgctga tccagggcgt ggtcaacacc ttcgtcgtat tcctgtcgcg cgtggtcgcg
      481 tttttcgtcg accgcatcgt ctttcgcacc gagcggggca atgggcccgg ctatttcatc
      541 acgatgatcg tcgcccagat cgtgctcggc gtgctggcga gcatgatcgt cgcgtggttc
      601 tcgcgccgac gcgaatttcg cgccgacgca ggcagcgcga ggctcctcgg gacgccgcag
      661 ccgatgatgc acgcgctggc ccgtctggga ggcctgccgc ccggcgagct gcccaagtcg
      721 atgaccggct tcggcattac cgatcactcc ggattcatgg ccctgttcgc cacccacccg
      781 ccgatcgagg agcgcatccg cgcgctgcag gcgggcgcgg cggcccgcta aaagcttcgc
      841 cggcgcgcac cgatggcggc tcccgtcaga tacaagatcg agatcgaggc tgcgaacggc
      901 atctggagtg acgtgcgcgg cggcgacggc gcggttctca cgttcgacag cgaagccgag
      961 gccagaacgc gtcttgccga gttgtacccg gtcgagacgc agatggagcg ttatggtgga
     1021 ggcaagcgca cgcgcgtcat tcgcatcctg cccgatgacg aggacgactg gccgaagcgc
     1081 ggcgcgccga gatagccgcc gcaccacgcg atctccgggc ctggctgaag aggccaatcg
     1141 atcgcggcag tgccgacaaa ctaacggccg gacatcgcga agcgatggcc ggccgtatga
     1201 tcggaccgct gtgtcgtcta cttgaggtgg ttcgagacca gcttggtcat ttcgaacatc
     1261 gacacctgcc gcttgccgcc gaaaacctgt cgcagcttgt cgtcggcatt gatcagtcgc
     1321 ctgttgatcg agtcctgcag cttgttcttc ttgatgtagt cccagatctt cttggtgact
     1381 tcggtgcgcg gcaacggatt gctgccgact accgcggcca gcgttgacga cggggtcatc
     1441 gccttcatga acgccgcgtt gggcttgcgc tttgctgcgg gcttcttggc ggctttcttc
     1501 ggggcggcct tctttgccgg tgcttttttc gctgctggtt ttttcgctgc ggatttcttc
     1561 gctgcgggct tctttgcagc cggcttcttc gcgggcttct ttgctttctt ggcagtagcc
     1621 atagcactcc ttcctggtgg ttaatcgctt cgatgcgacg gcgatgaata tggagtcagc
     1681 tcaatctcct gtcgttgctg ctttctccat cgaacaaata taccgcattc ccgcgggcat
     1741 agtatgcaaa ttgtgctcgg tttgcgaggg gcgcgtcagc attgttgcgc gattggttgg
     1801 ccgggacgag aagcgcggcg gcgggcgcgc cggtcccgac cgccgccgcg cgaacggccc
     1861 gccgcgcgcc gaccgctgtt gcgcgtgtca cgctgccctc ggccggtgcg ccgggcagcg
     1921 gctccggcgc cgcacgtgtc ggcgcgaaat cgccgataat gagcgaactc gcaccatgac
     1981 gccctcccag aacctcgccg aactgcgcag tgagtaccac cgccaagcgc tgaccgaagc
     2041 agacgtcgat cccgatccct tgcgccagct cgagcgatgg ctgcaggaag ctatccgcgc
     2101 cgcgctgccc gagccgaccg cgatgaccct ggccaccgtc ggggccgatg accgcccggc
     2161 agctcgcatc gtcctgctca agggagtcgg acccgacagc ctcgtttttt ttaccaacta
     2221 cgagagccgg aaagggcggg agctcgcgat tcatcccgat gccgcgttgc tctttcactg
     2281 gatcgagctc gagcggcagg tgcgaatcga gggcacggca agcaaggtgg cgggcgcgga
     2341 gtccgatgcc tatttcgcct cccgtccgag atcgtcgcgc ctcggcgcat gggcgtcgcc
     2401 gcaaagccgc accattcccg accgtgactg gctggagcgc gaattcgcgg cggtcgcggt
     2461 ccgctacgag ggcgacaacg cggcagtgcc gcgcccgccg ttctggggcg gctatcgcgt
     2521 cgtcccgtca ctggtcgaat tctggcaggg tcgcccctcg aggctgcacg accgcatcgt
     2581 ctatcggcgc gacggtccgc gctggcaggt cgagcggctc gcgccgtgac cccacccgca
     2641 gggcgcagcc gctacagctt tccggtgctg atcgcgctgg cgatcgtcaa tcactcggtg
     2701 ctggccggca gtcgggtgac cgtttcgctg tatgccctgt cgatcggcgc gtcgccgctg
     2761 gtcgtgggca cgctgatggg tctgtacgca tttctgccga tctggctgag tgtcgccgcc
     2821 ggccggctgt ccgatcgagt gggcgtgcgc gggccgatgc tggtcggctc gttcggagtc
     2881 gcgctgggcg ctgcgcttcc ctgcatctgg cccgggatcg gcgcgctgtt cgcggcgaca
     2941 tccttgatcg gcgcctcgtt catgctgttc caggtatcgg tgcaaaatgc caccggcgag
     3001 atgggaccgc cgggcgagcg cgccaagaat ttcagtctgc tggcgctcgg ctactcgatc
     3061 tcgggctttt gcggaccgct tgcggcggga ctctcgatcg accatctggg gttcggcacg
     3121 acgttcatcg gattcgcgct gctgccgctg ctgccggtac tggtattgac gcggcgcgct
     3181 ctcgtgcttc ccgggcgcca ccgggcccat gcgcaggcgc cttccggagg cattatgcag
     3241 ctgatgcgca atccgcagct caagcgcgta ttcattgtca atgcgctgct gtcgatggca
     3301 tgggacctgc acactttttt catcccgatc tacggcgcga caatcggatt gtcggcgtca
     3361 cgcatcgggg tcatcctcgc gtcgttcgct gcggcgacct ttgccgttcg cctgatgatg
     3421 ccgcggatcg cgcggagctt caccgaattc caggtgctga cggcggctat gttcatcgcc
     3481 gccgccacct acgcgctgtt cccgttcgtg gcgagcgcgg gcgcactgat ggtcctgtcg
     3541 tttgcgctgg ggctggcgct tggcagcgcg caaccgatgg taatggcgct gctgcacagc
     3601 atcgctccgg ctggcagaat gggcgaggcg gcgggcgtgc gcatgtcgat catcaatgcc
     3661 tcgacgttcg ccgtgccact gttgttcggc gcggtcggat cgacgctcgg att
//
LOCUS       DATANS010000329         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_438257, whole genome shotgun sequence.
ACCESSION   DATANS010000329 DATANS010000000
VERSION     DATANS010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438257"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VHT22_04750"
     CDS             complement(<1..190)
                     /locus_tag="VHT22_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX3136894.1"
                     /translation="MSTVPDAKRSGFTLLEILIAIAIVALLALMGYRALSALSESESR
                     LSAEATKWRTLDLFFARLE"
     gene            complement(187..594)
                     /gene="gspI"
                     /locus_tag="VHT22_04755"
     CDS             complement(187..594)
                     /gene="gspI"
                     /locus_tag="VHT22_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HEX3136895.1"
                     /translation="MEPRRADRPDSPLARTGFTLVEVLVALAIVAVTLAAGFRSVAQS
                     AESATALKSRTLALWVAQNRLAAAQLESPAPPSGERTGIETQAGAAFTWRETVSGTPN
                     PAFRKIEIDVGDSTRPDYTLARLVGYLGQAAQR"
     gene            complement(555..1067)
                     /locus_tag="VHT22_04760"
     CDS             complement(555..1067)
                     /locus_tag="VHT22_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX3136896.1"
                     /translation="MGPVAAPRFRVGRATGFTLAEILVVLIVIGLAAGLAYAHFETDP
                     RHTVERESQRLAAALEHAALLAQWKGETLGISASGNAYRFWRQPAGGLTWQPMSTDDV
                     LEPRLLPAPLDISAREYAGLPVAADAILPLRASGRNEPYVIQITSPEWFTLLAADPLN
                     RVALTGPIAR"
     gene            complement(1055..1513)
                     /gene="gspG"
                     /locus_tag="VHT22_04765"
     CDS             complement(1055..1513)
                     /gene="gspG"
                     /locus_tag="VHT22_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEX3136897.1"
                     /translation="MRQSLPSNAPHRIRSLRGFTLVEVLVVIVILGILAALVVPRVLE
                     RPDEARVIAAKNDIAAISQALKLYRLDNGRYPTGDQGLAALVAKPVQPPVPPNWKPNG
                     YLERLPKDPWGNNYQYINPGLRGEYDVFSFGADGAPGGTGFDADIGSWDL"
BASE COUNT          261 a          499 c          514 g          261 t
ORIGIN      
        1 cttcgagacg cgcgaagaaa agatcgagcg tgcgccattt cgttgcctcc gccgacaagc
       61 gtgactcgct ttcggacagc gccgacagcg cgcggtagcc catcagcgcg agcagcgcga
      121 cgatcgcgat cgcgatcaga atttccagca gcgtgaagcc cgatcgcttc gcatcgggaa
      181 cagtgctcat cgctgcgctg cctgtcccag gtagccgacc agtctcgcca gcgtgtaatc
      241 gggtcgcgtt gagtcgccga catcgatctc gatcttgcga aaggccggat tcggcgttcc
      301 gctgacggtt tcacgccagg tgaatgcggc gccggcttgc gtctcgatgc cggtgcgctc
      361 gcctgacggc ggcgccggcg attcaagctg tgcggcggcg agtcgattct gtgcgaccca
      421 gagggccagg gtgcgcgatt tgagcgcagt tgcgctttct gcgctctgcg cgacgctgcg
      481 aaacccggcc gcaagcgtca ccgcgacgat cgccagcgcg accagcacct cgaccagcgt
      541 gaagccggtg cgcgctagcg ggctatcggg ccggtcagcg cgacgcggtt caatggatcg
      601 gcggcgagca gcgtaaacca ttcgggcgag gtgatctgga tgacataggg ctcgttgcga
      661 ccgctggcac gaagcggcag gatcgcgtcg gcggcaacgg gcaaccccgc atattcgcgc
      721 gccgagatgt cgagcggcgc gggcaggagg cgcggctcaa ggacgtcgtc tgtcgacatc
      781 ggctgccatg tcaagccccc ggcgggctgc cgccagaatc gataggcgtt gccgctcgcg
      841 gagatgccca gcgtttcgcc tttccattgc gctagcagcg ccgcatgctc gagtgccgcg
      901 gccagtcgct ggctttcgcg ctcgacagtg tgccgaggat ccgtctcgaa atgcgcgtag
      961 gcgagtcctg cggccagccc gatcactatc agcacgacca gaatttccgc cagcgtgaac
     1021 ccggtcgcgc ggccgacgcg gaatcgcggc gcggctacag gtcccacgag ccgatgtcag
     1081 cgtcgaatcc cgtgccgccc ggcgcgccgt cggcgccgaa gctgaacacg tcgtactctc
     1141 cgcgcagccc gggattgatg tactggtagt tgttgcccca agggtccttc ggcagccgct
     1201 ccaggtaacc attgggcttc cagttcggcg gtaccggcgg ctgcaccggc ttggccacca
     1261 gggcggcgag gccctgatcg ccggtcggat aacgcccatt gtccaggcga tagagtttga
     1321 gcgcctggct tatcgcggcg atgtcgtttt tcgccgcgat gactctcgct tcatccggac
     1381 gctcgaggac gcgcggaacg accagcgccg cgagaatgcc gaggatgacg atgacgacca
     1441 ggacttcgac cagcgtaaag ccacgcaacg atcgaatccg gtgcggcgcg ttcgacggca
     1501 agctttggcg catgcagcca ttataatcgc ccggg
//
LOCUS       DATANS010000330         5096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_731372, whole genome shotgun sequence.
ACCESSION   DATANS010000330 DATANS010000000
VERSION     DATANS010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731372"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1143
                     /gene="cydB"
                     /locus_tag="VHT22_04770"
     CDS             142..1143
                     /gene="cydB"
                     /locus_tag="VHT22_04770"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX3136898.1"
                     /translation="MALDLVPLWAGILALAVFMYVLLDGFDLGVGILSPFAPDDASRD
                     QMMDSVAPVWDGNETWLVMGGLALLAAFPLAFAVIFPAVYFPILVMLIGLLFRGVAFE
                     FRHMSTRKHLWNWSFHAGSLVATFAQGIVLGTYVQGIPVEGRHFSGGSFEWATPFAVL
                     AGVGLIAGYGLIGACWLVMKTEGELQNWARGKARLLALGVAAFIAMVSVWTPLIQPQI
                     QHRWFAWPNLLLLSPVPVITLALFVWLLRALKSDREATPFLLALGLFGMCYLGLGISI
                     LPMIVPYSVTLWAAASSPKSQAFLMIGTLFLLPIIVAYTGWSYWVFRGKVRASEGYH"
     gene            1263..2144
                     /locus_tag="VHT22_04775"
     CDS             1263..2144
                     /locus_tag="VHT22_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3136899.1"
                     /translation="MSSRLGAGPVQLCIAACLALCLGTALAATEPVRTLERIRSDGVI
                     RLGYRSNATPFSFRERDGTVRGYSVELCDRIAAGIQKQLGLTKLRMEWTPLDAGDRLD
                     AVAKGRVDLECGTTTISLSRYERVDFSLPIFVDGGSVLTSVDAKLAQLKDLGGKRVAV
                     IPGTTTETALKRELAIVGANAELVPVADPPAGLALLASGKVVGYASDRIVLISLRARS
                     SNPSQFVLLDSDFSFEPYALVLRRDDPDFRLAVNRALTEVYRSGDIDAIFYRWLGGLG
                     KPGTLLNSMFYLNTLPE"
     gene            2141..2629
                     /locus_tag="VHT22_04780"
     CDS             2141..2629
                     /locus_tag="VHT22_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136900.1"
                     /translation="MMRAYQRGLARRAVAVLLLAGAASPLAAQTGTPLPPPNAPKPTP
                     TEACDRCGTITSIRQAETKDSWTRLGTVSSTGAGTSDLAPMGVTQYQVGRDLSKQGTV
                     VLGAAGGVAYQSRPSSLNAQRWELLVRMDDGSVRTVTQNYQPMLQSGDRVRVFGTQVE
                     LL"
     gene            2629..3789
                     /locus_tag="VHT22_04785"
     CDS             2629..3789
                     /locus_tag="VHT22_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HEX3136901.1"
                     /translation="MTAAAGAMTASAPAATLTVSDAAAGSRELRLAGRLDAYSIAGVW
                     HDTLAALAAAPGRAVVIDVSGVDYCDGAGIAMLIDLLRQPRAANAPVSLSGLRPEFQA
                     LLDQFDPVAMQVPVEPPHVTRINVIAEIGRAAAITGRDIRAQVAFIGETTTALWSAAK
                     HPARVRWKDVWYTCEQVGVNALPIVALISFLLGIILAFQAAVPMRQFGAELFVADLVG
                     LSILRELGPLMTAILLAGRSGAAFAAEIGTMKVNEELNALTTMGLDPVRFLVVTRIIA
                     AVLMMPLLTLFADLIGILGGALTMLTFNIPIASFLHEVDSLVDVKDLLAGMAKAPVFA
                     ILIAGVGCLRGLQTQTGASAVGISATRAVVSSIVLLVVVDGIYAFVYYLLDI"
     gene            3800..4564
                     /locus_tag="VHT22_04790"
     CDS             3800..4564
                     /locus_tag="VHT22_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018863866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3136902.1"
                     /translation="MPADPIITVEHLTAGYEGNVLMQDLNFSVHRGEVFIILGGSGSG
                     KSTLLKHMIGLYQPMGGSIRIEGDDIVTAEGAARRAILNKIGVMYQSGALFGSMTLLE
                     NVRLPLEEYTALDGAAMDLIARMKLQLVGLDAFCGHMPAEISGGMQKRAAIARAMALD
                     PMILFLDEPSAGLDPITSAELDQLILRLAKSLRITFVVVTHELASIYTIADRVIMLDK
                     RTKGIIAEGDPKVLRDTSDNPWVRRFFRREVAAEPA"
     gene            4570..>5096
                     /locus_tag="VHT22_04795"
     CDS             4570..>5096
                     /locus_tag="VHT22_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX3136903.1"
                     /translation="MSAQANFFKIGLFVIGGTVALVLLLLILGSGQLFQSKITMETYL
                     NESVQGLEVGSKVRYRGVVVGEVRRIGFTYNTYEQDQPMAARLRYVMVEATILPRLIG
                     GRAGGDITRLETARVEIDRGLRVRLAPQGITGTSYLEIDYVDPKANPPLPITWTPNNL
                     YIPSAQSTVTAFIGAA"
BASE COUNT          822 a         1629 c         1687 g          958 t
ORIGIN      
        1 cctcatcttc ggcatcggcg ggtttttcat ggtgcgcttc ctgcgcgcgg gcccggtcga
       61 caaggcgccg gacgccgggc gccgcggcca aggcacgccg gcccggccga tgtcggcggc
      121 ggaagtcgaa tagggggccg agtggcactc gatctcgttc cgctttgggc cggcattctc
      181 gcccttgcag tgttcatgta cgtgctgctg gacggcttcg atctgggcgt cggcatcctg
      241 tcgccgttcg cgccggatga tgcgagccgc gaccagatga tggattcggt cgcacccgtc
      301 tgggacggca acgagacttg gctggtcatg ggagggctcg cgctgctcgc cgcttttcct
      361 ctggccttcg ccgtcatctt cccagcggtg tacttcccga tcctggtcat gctgatcggg
      421 ttgttgtttc gcggcgtggc gttcgaattt cgccacatgt cgacgcgcaa gcacttgtgg
      481 aactggtcgt tccacgcggg atcgctggtc gccaccttcg cgcaggggat cgttctgggc
      541 acttacgtgc agggcatccc cgtcgagggc cggcatttca gcggcggcag cttcgaatgg
      601 gcgacgccgt tcgcggtgct cgccggtgtc ggactgatcg caggctacgg cctgatcggc
      661 gcctgctggc tggtgatgaa aacggaaggc gaactgcaaa actgggcgcg cggcaaggca
      721 agactgcttg cgctgggggt cgccgcattc atcgcgatgg tcagcgtgtg gacaccgctg
      781 atccagccgc aaatccagca ccgctggttc gcgtggccca accttctgct gctatcgccg
      841 gtgccggtga tcacgctggc attgttcgtc tggctgctgc gtgcgctcaa atcggaccgt
      901 gaagcgacgc cgtttctgct cgcgctgggg cttttcggga tgtgctacct gggcctggga
      961 atcagcattc tgccgatgat cgttccttac tcggtcacgc tctgggcggc ggcatcttcg
     1021 ccgaaatcgc aggccttcct gatgatcggc acattgttcc tgctgccgat catcgtcgcg
     1081 tataccggat ggtcgtactg ggtgtttcgc ggcaaggtgc gggcgagcga gggctatcac
     1141 tgacgccggt cagcggcgat cggggcgagt gtagcagcgc gccggcgcgt gctagacttc
     1201 gacgcacctg ccggcccgtc ggccactcgc ggcacctgca ggctcaccat cgggggagaa
     1261 cgatgtcgag tcgccttggc gccggcccag tccagctttg catcgcggcg tgccttgccc
     1321 tgtgcctggg caccgcgctt gccgcaaccg agccggtgcg cacgctggag cgaattcgca
     1381 gcgacggcgt gatccgcctc ggctatcgca gcaacgccac gccattctcg ttccgggagc
     1441 gcgacggcac ggtgcggggc tacagcgtcg agctgtgcga tcgcatcgcc gcggggatcc
     1501 agaagcagct cgggctgacg aagctcagga tggaatggac gccgctcgac gctggcgacc
     1561 ggctcgacgc ggtggccaag ggcagggtcg atcttgaatg cggcacgacg acgatctcgc
     1621 tttcgcgtta cgagcgggtg gacttcagcc tgccgatatt cgtcgacgga ggcagtgtgc
     1681 tgaccagcgt cgacgcgaag ctggcacagc tcaaggatct cggcggcaag cgcgtggcag
     1741 tgatcccggg gacgaccacg gagacggcgc tcaagcgcga gcttgccatc gtcggtgcaa
     1801 acgccgagct cgtcccggtc gccgatcccc ccgcgggcct cgcgctgctc gcttcgggca
     1861 aggtcgtcgg ctatgccagc gatcgcatcg tactgatatc gctgcgcgcc cgcagctcga
     1921 atccgtcgca attcgtcctg ctggacagcg atttttcgtt cgagccttac gcgctggtgc
     1981 tgcgccgcga cgaccccgat ttccggctgg cggtgaatcg cgcgttgacc gaagtctatc
     2041 gaagcggtga catcgacgcc atcttctatc gctggctcgg agggctcggc aagccgggta
     2101 cgctgctcaa ttcgatgttc tacctcaaca cgttgcccga atgatgcgcg cctaccaacg
     2161 aggacttgca cgccgcgcag tcgcggtact cctgctcgcc ggcgcggcgt cgccgcttgc
     2221 cgcgcagaca ggcactccac tgccaccgcc gaacgcgccg aagccgaccc cgaccgaagc
     2281 ctgtgatcgc tgcggaacga tcacatcgat tcgccaggcc gagaccaagg acagctggac
     2341 gcggttgggc accgtgtcat ccaccggtgc cgggacttcc gacctcgctc cgatgggcgt
     2401 cacgcaatac caggtgggac gtgacttgag caagcaggga actgtggtgc ttggtgcagc
     2461 cggaggtgtg gcataccagt cgcgcccctc ctcgctgaac gcgcagcggt gggaacttct
     2521 cgtgcgcatg gacgacggct cggtgcgcac ggtgacgcag aactaccaac cgatgctgca
     2581 gtcgggcgac cgcgtccgcg tcttcggtac gcaggtcgag ctgctgtagt gaccgctgcc
     2641 gcaggcgcga tgaccgcatc ggcgccggcg gcgacgctga cggtgtccga tgccgcggcg
     2701 ggcagccgcg agttgagact cgccggccgg ctcgacgcct attcgatcgc cggcgtctgg
     2761 cacgacacgc tcgccgcgct ggctgccgct cccggacgcg ccgtcgtcat cgacgtatcg
     2821 ggtgtcgatt attgcgacgg cgcgggcatc gcgatgctga tcgacctgct gcggcaaccg
     2881 cgcgcggcga atgcgccggt atcgctgtcc ggattgcggc cggaattcca ggcgctgctc
     2941 gaccagttcg atcccgtggc gatgcaagtg ccggtcgagc caccgcacgt tacgcggatc
     3001 aacgtcattg cggagatcgg acgcgcggcg gccatcaccg gccgcgacat acgcgcgcag
     3061 gtcgcgttca taggcgaaac cacgaccgcg ctgtggtctg cagccaagca tccggcacgg
     3121 gtccggtgga aggacgtctg gtatacctgt gaacaggtag gcgtcaacgc tctgccgatc
     3181 gtggcgctga tctctttttt gctcggcatc attctggcct tccaggccgc ggtgccgatg
     3241 cgccagttcg gcgccgagct ttttgtcgcc gatctggtcg ggctgtcgat cctgcgcgag
     3301 ctcgggccgt tgatgaccgc gatcctgctt gccgggcggt cgggtgccgc gttcgccgca
     3361 gagatcggca cgatgaaagt gaacgaggag ctgaacgctc tgaccacgat gggcctcgat
     3421 ccggtccgtt ttctggtcgt gactcggatc atcgcggcgg tgctgatgat gccgctgctt
     3481 acgctgttcg ccgacctgat aggcattctc ggcggcgcac tgacgatgct caccttcaac
     3541 atcccgattg ccagcttcct gcacgaggtc gacagcctgg tcgatgtgaa agacttgctt
     3601 gccgggatgg cgaaggcgcc ggtgttcgcc atcctcattg ccggtgtcgg ctgcctgcgc
     3661 ggtctgcaga cgcagaccgg ggcgagcgcc gtcggcatat ccgcaacgcg tgcggtcgtt
     3721 tcgagcatcg tcctgctggt tgtcgtcgac ggcatctacg cgttcgtcta ctatctgctc
     3781 gacatttgac gcacacccta tgcccgccga tccgatcatc accgtggagc acctgactgc
     3841 cgggtacgaa ggcaacgtgc tgatgcaaga cctcaacttc agcgtccatc gcggcgaggt
     3901 cttcatcatc ctgggcggct cgggcagcgg caagagcacg ctgctcaagc acatgatcgg
     3961 gctctaccag ccgatggggg gaagcatccg gatcgagggc gacgatatcg tcacagccga
     4021 gggtgcggct cggcgcgcga tcctgaacaa gatcggagtg atgtaccaga gcggcgcgct
     4081 gttcggctcg atgacgctgc tcgaaaacgt acggctgccg ctggaggaat atacggcgct
     4141 ggacggtgcg gcgatggacc tgatcgcgag aatgaaactg cagctggtcg ggctcgatgc
     4201 cttctgcggt cacatgccgg ccgagatcag cggaggcatg caaaagcgcg ccgcgatcgc
     4261 gcgcgcgatg gcgctcgacc cgatgatcct gttcctcgac gagccatcgg ccggcctcga
     4321 cccgatcacc tcggccgagc tcgaccagct gatcctgcga ctggcgaaga gcctgcgcat
     4381 caccttcgtc gtcgtcacgc acgagctcgc ctcgatttac acgatcgccg atcgggtgat
     4441 catgctcgac aaacgcacca aaggcatcat cgccgaaggc gatccgaagg tgctccgcga
     4501 caccagcgac aatccgtggg tgcggcgatt cttccggcgc gaagtggccg ccgaaccggc
     4561 gtaacggcga tgagcgcaca agccaacttc ttcaagatcg gcctgttcgt catcggcggg
     4621 acggtggcgc tggtgctgct gctgctgatc ctcggctccg gccagctgtt ccagtcgaag
     4681 atcacgatgg agacctatct gaacgagtcg gtccagggcc tcgaggtcgg ctcgaaggtc
     4741 agataccgcg gcgtagtggt cggcgaagtg aggcgcatcg gattcaccta caacacctac
     4801 gagcaggatc aaccgatggc ggcgcgtctg cgctacgtga tggtcgaggc gactatcctg
     4861 cctaggctga taggcggtcg tgcaggcggc gacatcacgc gccttgagac ggccagggtc
     4921 gagatcgatc gcggcttgcg cgttcggctc gcgccgcagg gcattaccgg gaccagttat
     4981 ctcgaaatcg actacgtcga tccgaaggca aaccctccac tgccgatcac ctggacgcct
     5041 aacaatctct acattccgag cgcgcagtcg acggttaccg ctttcatcgg cgccgc
//
LOCUS       DATANS010000331         1568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_689528, whole genome shotgun sequence.
ACCESSION   DATANS010000331 DATANS010000000
VERSION     DATANS010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689528"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..530
                     /locus_tag="VHT22_04800"
     CDS             294..530
                     /locus_tag="VHT22_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEX3136904.1"
                     /translation="MVNPEQVKAYIEQGLECEFVSVAGDGHHFEAVIVSAAFNGKNKV
                     QQHQIVYRALGDRMREEIHALSMQTHTPADWAKR"
     gene            549..>1568
                     /gene="murA"
                     /locus_tag="VHT22_04805"
     CDS             549..>1568
                     /gene="murA"
                     /locus_tag="VHT22_04805"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX3136905.1"
                     /translation="MDKLVIEGGVALKGEVRISGAKNAALPILCAAILSDEPLRISNV
                     PHLRDVTTMLGLLAQMGIAVSVDDKLGIELNAQALDNPAAPYELVKTMRASVLVLGPL
                     LARCGKARVSLPGGCAIGLRPVDQHLKALQAMGATIVIEHGYMQASAPRLKGARIIMD
                     LVTVTGTENIMMAATLADGETTIENAAREPEVVDLAECLKAMGAQIKGAGSDVIRITG
                     VTRLKAASYRVMPDRIETGTFLAAAAATGGEVRLRDTRANILDAVLEKLREAGAHIEK
                     NEDSISLGMAGGLRAVNLRTAPYPAFPTDMQAQFMALNTVADGTALITETIFENRFMH
                     VQELRR"
BASE COUNT          310 a          478 c          527 g          253 t
ORIGIN      
        1 tgcgttgagc gttttattcg agtggcggcg cgctgcccgc gacaagaacg tgcatgtctc
       61 gtttcgcaat ctgccggaca gcatgagtag cctcgcggca ctgtacggcg tcgcggagct
      121 ggttgcgccg gacgcctgaa cgcagggcgc ggcgcccgcg ccggcagttt cgcccgtagc
      181 ccgcatgatt cccgcgattc aagcgaacgc cccgtctagg gccatggcgc tgatgcttta
      241 cgccgttggc tggtcgaaac cgggtataaa ctgcggcact gaggactgaa cctatggtaa
      301 atcccgagca ggtaaaagcc tatatcgagc aggggctcga atgcgaattc gtcagcgtgg
      361 ccggcgacgg ccaccacttc gaagccgtaa tcgtgagcgc tgcgttcaac ggcaagaaca
      421 aggtgcagca gcaccagatt gtctatcgcg cgctgggcga tcgcatgcgc gaggaaatcc
      481 acgcgctgtc gatgcagacg catacgcccg ccgactgggc caagcgctga tcagcgccgg
      541 tccgccgcat ggataaactg gtcatcgagg gcggggtcgc gctcaagggt gaagtgcgga
      601 tttccggcgc caagaacgcg gcgctgccga tcctgtgcgc ggcgatcctg agcgacgagc
      661 cattgcgcat ttcgaacgtg ccgcatttgc gcgacgtcac gaccatgttg gggctgctcg
      721 cgcaaatggg catcgccgtc tcggtcgacg acaagctcgg catcgaattg aacgcgcagg
      781 cgctcgacaa tccggcggcg ccctacgagc tcgtaaagac gatgcgggcg tcagtgctgg
      841 tgctcggtcc tctgctcgca cgctgcggca aggcgcgcgt gtcgctgccc ggcggttgcg
      901 cgatcggact gcggcccgtc gatcaacatc tgaaggcgct gcaggcgatg ggcgcgacga
      961 tcgtaatcga gcacggctat atgcaggcga gcgcgccgcg cctcaagggg gcgcgcatca
     1021 tcatggatct ggtgacggtc accggcaccg aaaacatcat gatggcggcg actctcgccg
     1081 acggcgagac gaccatcgaa aacgcggcgc gcgagccgga agtcgtcgat ctcgccgaat
     1141 gcctgaaggc gatgggcgca cagataaaag gcgccggcag cgacgtgatc aggatcaccg
     1201 gcgtgacgcg cctcaaagca gcgagctatc gcgtaatgcc ggaccggatc gagaccggca
     1261 cgttcctggc ggcggccgcg gcaaccggcg gggaagtgcg cctgcgcgac acgcgcgcga
     1321 acatactcga tgcggtgctc gagaaactgc gtgaagcggg cgcgcacatc gaaaagaacg
     1381 aggactcgat cagcctgggc atggcgggtg ggttgcgcgc ggtgaacctg cgcacggcgc
     1441 catatcccgc attcccaacc gacatgcagg cgcaattcat ggcgctcaat actgtggctg
     1501 atggcactgc gctgataacc gaaacgatct tcgagaaccg cttcatgcac gtgcaggaat
     1561 tgcgccgg
//
LOCUS       DATANS010000332         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_438548, whole genome shotgun sequence.
ACCESSION   DATANS010000332 DATANS010000000
VERSION     DATANS010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438548"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VHT22_04810"
     CDS             <1..909
                     /locus_tag="VHT22_04810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3136906.1"
                     /translation="VAIETVRPLIDAQEHALSVLLPEQPMRIRGDFARLAQVLANLLN
                     NAAKYTDRKGRIALTAEQEGAEVVFRVRDSGIGIPASALPTIFELFTQVEQTLDRSQG
                     GLGIGLTLVERLVKMQGGSVSAFSAGKNLGSEFTVRLPAMPVDQRVAAGRRAAERYTA
                     SSPGEFAILIVDDNRDATDSMAMLLAMEGYDVRVAYDGPQALEAVRTARPDVILLDLG
                     LPGMDGFQVAQRVRADPDNSSIVIVAVSGYGQEEHRSRSSQAGCDHHLVKPIEPAVVS
                     ELLASLQSRRHGASPENIVRLRRSAD"
     gene            1077..1832
                     /locus_tag="VHT22_04815"
     CDS             1077..1832
                     /locus_tag="VHT22_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEX3136907.1"
                     /translation="MSDTSHRFPNREGQRVPDVSLRIRRNGEWATVTSADLFANKHIV
                     VFSLPGAFTPTCSSTHLPRYNELAPAFKAAGIDAIVCVSVNDPFVMEAWAKDQEAANV
                     TLLPDGNGEFTRKMGLLVDKSELNFGQRSWRYSMLVRDGVIEKMFIEPDEPGDPFVVS
                     DADTMLTYLNPKAKKPDQVAILTREGCSFCAKAKRLLTEAGIAYAEVLLDHAVRTKVI
                     GAITGAQTVPQVFINGRLIGNAEDLERHLKRAA"
     gene            1878..>2351
                     /locus_tag="VHT22_04820"
     CDS             1878..>2351
                     /locus_tag="VHT22_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013814984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3136908.1"
                     /translation="MQILDTDVAIIGAGTAGLAAYRTVKAAGQRALLIEGGPYGTTCA
                     RVGCMPSKLLIAAAEAAHAFSRFPAFGIRPAGPIAIDGKAVMDRVKGERDRFAGFVVD
                     DIEAFPPADKIRGHARFIDDHTLQVGDALTIRAPRIVIATGSSPAHPAAWDALGDR"
BASE COUNT          427 a          779 c          762 g          383 t
ORIGIN      
        1 gtcgccatcg agaccgtgcg accgctgatc gacgcgcagg agcatgcgct gagcgtgctg
       61 ctgcccgagc agccgatgcg tatccgaggc gatttcgcgc gactggcgca ggtgctggcc
      121 aacctgttga acaacgcggc caagtacacc gatcgcaagg ggcggatcgc gctgaccgcc
      181 gagcaggaag gcgccgaagt cgtcttccgc gtccgcgatt cgggcatcgg catcccggct
      241 tcggcgctgc cgacgatctt cgagcttttc acccaggtcg aacagacgct ggaccgctcg
      301 cagggcggtc ttggcatcgg gctgacgctg gtcgagcggc tggtcaagat gcagggtgga
      361 agcgtgtcgg cattcagcgc cggcaagaat ctcggcagcg aattcaccgt gcgcctgccg
      421 gcgatgcccg ttgatcagcg cgtcgcggcc ggacgccgcg cggcggagcg ctacaccgcc
      481 tcgagccccg gcgagttcgc gatcctgatc gtcgacgaca accgcgacgc gaccgacagc
      541 atggcgatgc tgctcgcaat ggaaggctac gacgttcgcg tcgcctacga cggaccgcag
      601 gcgctggaag cggtgcgcac cgcgcgaccg gacgtcatcc tgctcgacct gggcctgccc
      661 ggcatggacg gctttcaggt cgcgcagcgg gtgcgcgccg atccggacaa cagctcgatc
      721 gtcatcgtcg cggtctccgg gtacggacag gaagagcacc gctcgcgctc gagccaggcc
      781 ggttgcgatc accatctggt caagccgatc gagcccgccg tcgtcagtga gctcctggcc
      841 tcgctgcagt cgcggcggca cggggcgtcg ccggaaaaca tcgtccgcct gcgcaggagc
      901 gccgactagc aggatctctc cttcggcccc gcgtcgcgcc gcgcgccaag acgcctgccg
      961 ccttataatc gatagcctac cgctatcgat atgatcgaat aaataaaatt gaactatcgc
     1021 tctcgctcag cgatcatatc gatcgtcaat cgaccaaact gaagagagca accaccatgt
     1081 ccgataccag ccatcgattt ccgaaccgcg agggccagcg cgtgcccgac gtttcgctgc
     1141 gcatccgccg caacggcgag tgggccacgg tcaccagcgc cgacctgttc gcgaacaagc
     1201 atatagtcgt gttttcgttg cccggcgcgt tcacgccgac ctgctcatcg acccatctgc
     1261 cccgctacaa cgagctcgcg ccggcattca aggccgccgg gatagacgct atcgtctgcg
     1321 tctcggtcaa cgatccgttc gtgatggaag catgggcgaa agaccaggag gcagccaacg
     1381 tgacgctgct gcccgacggc aacggcgaat tcacgcgcaa aatgggcctt ctggtcgaca
     1441 agagcgagct caacttcggt cagcgctcgt ggcgctactc gatgctggtg cgtgacggcg
     1501 tgatcgagaa gatgttcatc gagccggacg agccgggcga cccgttcgtc gtctccgatg
     1561 ccgataccat gctcacctac ctgaacccga aggcgaaaaa gcccgatcag gtggccattc
     1621 tgacgcgtga aggctgctcg ttttgcgcca aggcgaagcg gctgctgacc gaagcgggga
     1681 tcgcatacgc cgaggttctg ctcgaccatg cggtccgcac caaggtcatc ggcgcgatca
     1741 ccggagcgca aacggtgccg caggtcttta tcaacgggcg tttgatcggg aacgccgagg
     1801 acctcgagcg ccatctgaag cgggcggcgt aagctagggc ttgtaaccga ctttgcgtcg
     1861 ggggaggaga cgtgcccatg cagatccttg acaccgacgt cgccatcata ggtgccggca
     1921 ccgcaggact tgccgcatac cggaccgtca aggcggccgg acagcgcgcg ctgctgatcg
     1981 agggcggccc ctacggcacg acgtgcgcgc gcgtcggatg catgccgagc aagctgctga
     2041 tcgcggcggc ggaagccgcg cacgctttct cgcgtttccc tgcgttcggc attcggccag
     2101 cgggcccgat cgccatcgac ggcaaggcgg tgatggatcg cgtcaagggc gagcgagacc
     2161 gcttcgccgg atttgtcgtc gacgacatcg aagcgtttcc gccggccgac aagattcgtg
     2221 gccacgccag gtttatcgac gaccacacac tgcaggtcgg cgacgcgctg accatccgcg
     2281 cgccgcgcat cgtcatcgcc accggatcgt cgccggcgca tcctgcggcg tgggacgcgc
     2341 tcggcgaccg g
//
LOCUS       DATANS010000333         4599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_143689, whole genome shotgun sequence.
ACCESSION   DATANS010000333 DATANS010000000
VERSION     DATANS010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143689"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VHT22_04825"
     CDS             <1..211
                     /locus_tag="VHT22_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEX3136909.1"
                     /translation="STNTALWNRQEEDVDINCGEIVDGTASVDEIGERFFRMILDTAS
                     GQKSKSELHGYGQNEFVPWYLGAVM"
     gene            284..1363
                     /gene="ugpC"
                     /locus_tag="VHT22_04830"
     CDS             284..1363
                     /gene="ugpC"
                     /locus_tag="VHT22_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX3136910.1"
                     /translation="MASVSFHDIEKSFGSTKVIHGIGFDIKDGEFMVLVGPSGCGKST
                     LLRMLAGLEEITAGTIAIDSKVVNDVESKDRDIAMVFQSYALYPHMTVADNMAFSLKL
                     RKADAKTIEERVARAAKILNLDPYLHRYPRELSGGQRQRVALGRAIVRDPKVFLFDEP
                     LSNLDAKLRVAMRAEIKALHQRLKTTTVYVTHDQVEAMTMADRIVVMHDGRIEQIGTP
                     LDLYDRPDNLFVAQFIGSPAMNIVNGRLRRGSGAAFVEADGGLRWPLGSTAGADGQAV
                     AYGVRPEHLSLAEGNGADAVPAEITVVEPTGAETELLVQAGESQIVLVTHGRPQVNPG
                     DKIGLRVDPAMVHVFDQATGQRLSA"
     gene            1432..3168
                     /gene="araD"
                     /locus_tag="VHT22_04835"
     CDS             1432..3168
                     /gene="araD"
                     /locus_tag="VHT22_04835"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HEX3136911.1"
                     /translation="MAKKSRKPASPLRSTAWFGRHDRDALVHRSWMKNQGIPHDQFDG
                     RPVIGICNTWSQATPCNAHFRELAEHVRSGVLDAGGFPIEFPVMSLGETLMRPTTMLF
                     RNQVAMDVEESIRANPFDGVVLMMGCDKTTPALLMGAASCDLPTIGISGGPMLNGKFR
                     GEDIGSGTHVWKFTEMLKTGAMKESDMVEVESCMSRSAGHCMTMGTASTMASMVEALG
                     IGLPTNAAIPAVDSRRRVLARMAGRRIVDMVREDLRMSKILTKDAFENAIMVNGAIGG
                     STNAVVHLLAVAGRIGVQLSLDDWDRLGHDMPCLVNLMPSGKYLMEDFYYAGGLPVVI
                     KAIGKFLHKKALTANGRTLWDNVKDAVNYNPAVITPLAKPFKPQGGIAVLRGNLAPGG
                     AVLKPSAASPKLLKHKGRAVVFEDIDDLHRRIDDPKLDVRESDILVLKNCGPKGYPGF
                     PEVGNFTLPAKLLKKGVTDMVRISDARMSGTAYGTVVLHTAPEAAAGGPLALVKNGDT
                     IELDVARRRIHLDVSDAELARRKAKWKAPPPHAERGWVRLYCETVQQADQGVDLEFLV
                     GKSGAKVGRPSH"
     gene            3340..3498
                     /locus_tag="VHT22_04840"
     CDS             3340..3498
                     /locus_tag="VHT22_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136912.1"
                     /translation="MAKPNYQYQKRQKELEKKKKQEEKRKRKLEKTEADTAAEGATPP
                     VEGETPPA"
     gene            complement(3506..4140)
                     /locus_tag="VHT22_04845"
                     /pseudo
     CDS             complement(3506..4140)
                     /locus_tag="VHT22_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504739.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
     gene            complement(4174..4452)
                     /locus_tag="VHT22_04850"
     CDS             complement(4174..4452)
                     /locus_tag="VHT22_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136913.1"
                     /translation="MTSHRAPGRRNELTERLGRCYTGAVHDVLRMMGHERCVLPLGDR
                     DGVVIVPGRVVEETVARTEAVVATESEMRRALIGGMDPVDAYHKFGKF"
BASE COUNT          901 a         1443 c         1535 g          720 t
ORIGIN      
        1 ctcgaccaac accgcgctgt ggaaccggca ggaagaggac gtcgacatca actgcggaga
       61 aatcgtcgac ggcacggcat cggtcgacga aatcggcgag cgattcttcc gcatgatcct
      121 cgacaccgcg tcggggcaga agagcaagag cgaattgcac gggtacggac agaatgaatt
      181 cgtgccgtgg tatctgggag ccgtcatgtg atgccggctt ggactgacgg taaaatggct
      241 tgacctgacg ctgcctcggc cacttgcgga accggcaatc atcatggcat cggtatcgtt
      301 tcacgacatc gaaaaaagct tcggatcgac caaggtcatc cacggcatcg gcttcgacat
      361 caaggatggc gagttcatgg tcctggtcgg gccttccggg tgcggcaaat cgaccttgct
      421 gcgcatgctg gcgggtctcg aggagatcac cgcggggacg atcgcgatcg acagcaaggt
      481 cgtcaacgac gtcgagtcga aggaccgcga catcgcgatg gtgttccaga gctacgcgct
      541 ttatccgcac atgacggtcg cggacaacat ggcgttcagc ctgaagctgc gcaaggccga
      601 tgcgaagacg atcgaggagc gcgtcgcccg ggccgcgaag atcctgaacc tcgatcccta
      661 cctgcaccgc tatccgcgcg agctgtcggg cggacagcgg cagcgggtcg cgttgggacg
      721 cgcgatcgtg cgcgatccga aggtgttcct gttcgacgag ccgctgtcga acctcgatgc
      781 caagttgcgc gtcgcgatgc gggccgagat caaggcgctg caccagcggc tgaagacgac
      841 gaccgtgtac gtgacgcacg accaggtcga ggcgatgacg atggccgacc ggatcgtggt
      901 gatgcacgac ggccgcatcg agcagatagg aacgccgctc gatctgtacg accggccgga
      961 caacctgttc gtcgcgcaat tcatcggttc gccggcgatg aacatcgtca acggcaggtt
     1021 gcggcgcggc agcggcgcgg cgttcgtcga agccgatggc gggctacgct ggccgctcgg
     1081 ttcgaccgct ggggccgatg ggcaggccgt cgcctacggc gtccgccccg agcatctgtc
     1141 gttggccgag ggcaacggcg ccgacgcggt ccctgcggag atcaccgtcg ttgagccgac
     1201 cggcgccgaa accgagcttc tggtgcaggc gggcgagtcg cagatcgtgc tggtcacgca
     1261 cggacggccg caggtcaatc cgggagacaa gatcggcttg cgcgtcgatc cggcgatggt
     1321 gcacgtattc gatcaggcta ccggacaacg actttccgca tgactttggg ggcgcggccg
     1381 aagccatcgc ccctgacacg tttcccacaa ccgatcggac aaccgacgag catggcaaaa
     1441 aagtctcgca agccagcttc accgcttcgt tccaccgcgt ggttcggccg tcacgaccgc
     1501 gacgcgctcg tccaccgctc gtggatgaaa aaccagggca taccgcacga ccaattcgac
     1561 ggccggccgg tcatcgggat ctgcaacacc tggtcgcagg caacgccctg caacgcgcac
     1621 ttccgcgagc tggccgaaca cgtccgctcg ggcgtgctcg acgccggcgg ttttccgatc
     1681 gaattcccgg tgatgagcct cggcgaaacc ctgatgcggc cgacgacgat gctgttccgc
     1741 aaccaagtcg cgatggacgt cgaggaatcg atccgcgcca atccgttcga cggcgtcgtg
     1801 ctgatgatgg gctgcgacaa gacgacgccc gcgctgctga tgggtgcggc gtcgtgcgat
     1861 ctgccgacga taggcatctc cggcggaccg atgctgaacg gcaagtttcg cggcgaggac
     1921 atcggctccg ggacacacgt gtggaagttc accgagatgc tcaagaccgg tgcgatgaag
     1981 gaatcggaca tggtcgaagt cgaatcgtgc atgtcgcgct cggccggaca ctgcatgacg
     2041 atgggcaccg cctcgacgat ggcgtcgatg gtcgaagcgc tgggcatcgg cttgccgacc
     2101 aacgcggcga tcccggcggt cgactcgcgg cgcagggtgc tcgcgcgcat ggcgggccgg
     2161 cgcatcgtcg acatggtccg cgaggatctg cgcatgtcga agattctgac caaggacgcg
     2221 ttcgaaaacg cgatcatggt caacggtgcg atcggcggat cgaccaacgc cgtcgtccac
     2281 ctgctagcgg tcgccggacg catcggcgtc cagctgtcgc tcgacgattg ggaccggctc
     2341 ggccacgaca tgccctgcct ggtcaacctg atgccgtcgg gcaagtacct gatggaggac
     2401 ttctactatg ccggcggcct gcccgttgtc atcaaggcga tcggcaaatt cctgcacaag
     2461 aaggccttga ctgcaaatgg ccgcacgcta tgggataacg tcaaggacgc ggtcaactac
     2521 aacccggcgg tgatcacgcc gctggccaaa ccgttcaagc cgcagggcgg catcgcggtg
     2581 ctgcgcggca acctggcgcc gggcggtgcg gtgctgaagc cttcggcggc gagccccaag
     2641 ctgctcaagc acaaaggcag ggcggtggtg ttcgaagaca tcgacgatct gcaccggcgc
     2701 atcgacgatc cgaagctcga cgtgcgcgaa tccgacattc tggtgctcaa gaactgcggg
     2761 cccaaaggct atccggggtt ccccgaagtc ggcaacttca cactgccggc gaaactattg
     2821 aaaaagggtg tgaccgacat ggttcgaata tcggacgcgc ggatgtcggg tactgcgtac
     2881 gggacggtgg tgctgcatac cgcacccgaa gcggccgccg gcggaccgct tgcgctggtc
     2941 aaaaatggcg acacgatcga gctcgacgtc gccaggcgcc gtattcacct cgacgtctcc
     3001 gacgccgagc ttgcgcgccg caaggcgaag tggaaggcgc cgccgccgca cgccgagcgc
     3061 ggctgggtga ggctctactg cgagaccgtg caacaggccg accaaggcgt cgacctcgag
     3121 ttcctggtcg gcaagtccgg cgccaaggtg ggacgaccga gccattagca ttggtttcga
     3181 cgccgtgccc gcatcgggcc ggtggacggc gtgttagttc acgcgcagcg accgattgcc
     3241 cctacaatgg gggcagacgc ggcgttcgcg tcgcgccgac ggtatttcgg tgcgaggtcc
     3301 tcgaattcac cgtaactcga tcctacggga ggtctaccct tggcaaaacc caactatcag
     3361 taccagaaac ggcaaaagga actggaaaag aagaaaaagc aggaagaaaa gcgcaagcgc
     3421 aagctggaga aaacggaagc cgacaccgcc gccgagggcg caacgcctcc agtcgaaggc
     3481 gagacgccac cggcttgaag caggactagc tggcctggcg tacggtgagc gccagcttta
     3541 ctccgagtat cacgaacacg gcaccggcga ttcgatgcag tgcgtccatg cgcaccggga
     3601 tgccgttgaa gcgctggccg atccccgccg acgaccacgc gacgaacagg ttccacaaga
     3661 gtcccgtcgt attgaacacc gtgccgagca tcaggaacgc ggccagccga tgcgccccct
     3721 ccgggtcgac gaactgcggc aggaacgcca ggaagaacag cgcggattca ggatattggt
     3781 cagcgcgccc tgcacgaaga tgtgcgaaag cgatgtcgcc ggcatcggcg cggcagcaag
     3841 cttcggcgaa cgagggccgg tccgccacag cacgattccc atgtagatca ggtaggccgc
     3901 gccggcgagc ttgactgcgg tgaacgcgac ggccgacgtc gccagcagcg ccgacaggcc
     3961 cagtcccgcg gcgacgatgt gcacgcagca gcctgacgcg atgcccagcg ccgccacggc
     4021 gccggctttg cgtcccaacg tcgtcgcgcg agtgacgatg tagagcgtat cggcgccggg
     4081 cgtcaggttg agcaggatgc ccgaagcgac gaacagtgca aagtcatgga tgcctggcat
     4141 gcggtctcga gtgcgcggag gcgttggtgc gctctagaac ttgccgaact tgtggtaggc
     4201 gtccaccggg tccatgccgc cgatcagcgc acggcgcatc tcgctctcgg tcgcgacgac
     4261 ggcttcggtg cgcgcgaccg tttcctcgac cacgcggccc ggcacgatga cgacgccgtc
     4321 gcgatcgccg agcggcagga cgcagcgctc gtgccccatc atgcgcagca cgtcgtgcac
     4381 cgcgcctgta tagcagcgtc caagcctctc ggtcagctcg ttgcgccgcc cgggcgcacg
     4441 atgcgaggtc aagtcagaac agcgtcagtt gcgcgcgctg cgcggcactg accggcggct
     4501 tgaagcgcga ggtgtccatc ggcgcgcgct cgcggttgag atcgagtcgg cgcgcggcga
     4561 tgtcgaagcg gcgggcgatc agctcggcga attgtccct
//
LOCUS       DATANS010000334         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_689679, whole genome shotgun sequence.
ACCESSION   DATANS010000334 DATANS010000000
VERSION     DATANS010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689679"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..>2093
                     /locus_tag="VHT22_04855"
     CDS             247..>2093
                     /locus_tag="VHT22_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX3136914.1"
                     /translation="MSVESRVDLLDLRAESFIAQGDLKRATADADAMLDLAKGAGTAA
                     LNAQAHNRLALVQMRTGALKAAVASATAALKAARQSKQLALEATSLYRLAEAQYRSRV
                     DVEQAARHALRASALFHAQERPGDEGRALWLVAMARSNQGRSAEADKASNAALALCRQ
                     AGDLYGAGNALNMLIFNQADFAVQLKLLHQALADFEAAGYVERQAIITANMGIAYSRL
                     GLYRRARRLFLSANEIYRRTDARVSQANNFAALAEVELAMGHLDSAGAYIAEMTGMTE
                     ALATPQSAASVAMNEGWVALLQGNAAMALRCFERAEKLARESDHVAGQIDVLAAIGHT
                     RLVLGKPRAALTATRRATGLHRAHDLAALDGMSPALTWWRHSQALRSSKQPQAAREAL
                     EMAYQLMLKGIVSLSDEGLRRNYLSKIEAHREIVHAWIKDARKRRLSPQRRGAHLAGE
                     ANLREPFERLVDTGLRLNELRSSEELHEFLIDEATELSGAERVLLVLESGQGLRLAGS
                     LMPRGEDAQALLRDIEPSLAEVRRTRAVMLTYAPEGESELDQRSRIIAPLIAQRQLLG
                     YLYVDIDGAFGRFRESDRDLLGMLASQAAVALDNAQWSQGLEQKVEERTS"
BASE COUNT          388 a          661 c          699 g          345 t
ORIGIN      
        1 taatatccgg ttcgccgcgc ccgcattcgg gcccttgcgg cgcgccggca ggtcaccctt
       61 gcaatggatg gagacttcgt gcaattgatg atggattcga aggggaaaaa agcatcgcgg
      121 cgccggccaa aggcgccgcg cgcagatgct tctgccccga tcagggcacg cgtaagcgaa
      181 ctggcgtgga ccgggcacca cgcggaggcg atcgagcacg caagcgccgc tctggccaaa
      241 agcgacttga gcgtcgaaag ccgggtcgac ctgctcgacc ttcgagcgga aagcttcatt
      301 gctcagggtg acctgaagcg cgccaccgct gacgcggatg cgatgctcga ccttgccaag
      361 ggcgccggta cggcagcgct gaacgcgcag gcgcacaacc ggctcgccct ggttcagatg
      421 cgcacgggcg cattgaaggc ggccgttgcc tcggccaccg ccgcgctcaa ggccgcacgg
      481 cagagcaaac agcttgctct cgaagcgacg agcctgtatc ggctcgccga agcccaatac
      541 cgcagcaggg tcgatgtcga acaggcggcg cgccatgctt tgcgggccag cgcattgttt
      601 catgcgcagg aacggcccgg cgacgagggg cgtgcgctgt ggctggtcgc catggcgcgc
      661 agcaaccaag gccgctccgc cgaggcggat aaggcgtcca acgccgcgtt ggcgctatgc
      721 cggcaggctg gcgacctgta cggcgccggc aatgcgctca acatgctcat cttcaaccag
      781 gccgattttg ccgttcagtt gaagctcttg catcaggcac tcgccgattt cgaggccgcc
      841 ggctatgtcg aacgccaggc aatcattacc gcgaacatgg ggatcgctta cagccgtctt
      901 ggactgtacc ggcgcgcccg ccggctgttt ctaagtgcca atgaaattta tcgtcggacc
      961 gatgcacgcg tgtcccaggc caataatttc gccgcgctcg ccgaggtcga gctcgcaatg
     1021 ggacatttgg acagtgcggg cgcgtatatc gcagaaatga ccgggatgac agaagcgctc
     1081 gccactcccc aatctgccgc gagcgtcgcc atgaatgaag ggtgggttgc cctgcttcag
     1141 ggcaacgccg cgatggctct gcgttgtttc gaacgcgcgg agaagctcgc ccgcgagagc
     1201 gatcatgtcg ccgggcaaat cgatgtcctg gcggcaatcg gacatacccg cctggtgctc
     1261 ggaaagccac gcgccgcatt gaccgccacg cggcgcgcca ccggattgca ccgtgcacat
     1321 gacctggccg cgctcgatgg gatgtcgcct gcgttgacgt ggtggcgaca cagccaggct
     1381 ctgcggagca gcaagcagcc gcaggctgcg cgcgaggcgc tggagatggc ctatcaactg
     1441 atgctgaagg gcattgtcag cctcagcgac gaaggcctgc gccgcaacta cctgagcaag
     1501 atcgaggcgc atcgcgagat cgtccacgcg tggatcaagg acgcccgcaa gcgtcgcctg
     1561 tcgccgcagc ggcgcggcgc gcacctggcc ggcgaagcga atctgcgcga gccgttcgaa
     1621 cggctggtcg atactggtct tcggctgaac gaattgcgca gcagtgaaga gctgcatgaa
     1681 ttcctgatcg acgaagctac cgagctctcc ggcgccgagc gcgttctgct ggtgctggag
     1741 tcggggcaag ggctgcgact ggcggggtcg ctgatgcctc gcggcgagga cgcgcaggca
     1801 ttgttgcgcg acatcgagcc atcgctggct gaagtgcggc gtacgcgcgc cgtcatgctg
     1861 acctatgccc ccgagggtga aagcgagctc gaccagcgct cgcgtatcat cgcgccgctg
     1921 attgcgcagc gccagttgct tggctacctc tacgtcgata tcgacggtgc attcgggcgc
     1981 ttccgtgaat ccgatcgcga cctgctggga atgctcgcga gccaggctgc agtcgcgctc
     2041 gacaacgcgc aatggtcgca gggcctcgag cagaaggtcg aagagcggac gtc
//
LOCUS       DATANS010000335         3387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_16395, whole genome shotgun sequence.
ACCESSION   DATANS010000335 DATANS010000000
VERSION     DATANS010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16395"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VHT22_04860"
     CDS             complement(<1..551)
                     /locus_tag="VHT22_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3136915.1"
                     /translation="MSAKHHRLLILGSGPAGFTAAVYAARANLKPVLITGLAQGGQLM
                     TTTDVDNWPADSDGVQGPELMARFQKHAERFDTEIIFDHIHTAALRERPLRLVGDIGE
                     YTCDALIIATGASARYLGLPSEQAFMGKGVSACATCDGFFYKGQDVIVVGGGNTAVEE
                     ALYLSNIARQVSLVHRRNKFRAEA"
     gene            698..3016
                     /locus_tag="VHT22_04865"
     CDS             698..3016
                     /locus_tag="VHT22_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX3136916.1"
                     /translation="MIFKQKIQRQRRAVAAPEPTLSPRLAALVRESWWLLVVAVFAYL
                     ALILATYQRTDPGWSFSGTGAAIHNKGGAVGAWLADLLLYLFGISAWWWVFAGIVLVV
                     TGYRHLTRSIDAADRHHPWLAVPGFALVLLSSSALEALRLYRLPALLPHAPGGALGEM
                     IGSGFSRSLGFNGGTLLLIALFAIGWSLFTGMSWLRLMERVGAGIERLIASLRQRRDA
                     RRDREIGAVALEEREQFVEAARESIEDREPVVVVPAMVDVPKSERVVKEKQKPLFQDM
                     PDSPLPPLALLEDAPSAQEQVSPETLEFTSRLIERKLADFGVSVKVLAAYPGPVITRF
                     EIEPAIGVKGAQIVNLMKDLARALSVVSIRVVETIPGKSFMGLELPNAKRQIVRLVEL
                     LSSTTYNDTASPLSLALGKDIAGKPVIVDLARMPHLLVAGTTGSGKSVAVNAMILSLL
                     YKAEPRQVRLILIDPKMLELSVYEGIPHLLAPVVTDMKLAANALHWCVGEMERRYRLM
                     STLGVRNLSGYNARVVEAKKAGEPLTNPFTLTPESPEPIEEMPLIVVVIDELADLMMV
                     TGKKIEELIARIAQKARAAGIHLILATQRPSVDVITGLIKANIPSRIAFQVASKVDSR
                     TILDQMGAETLLGQGDMLYLPPGTGHPQRVHGAFVADTEVHRVVEHLKAQGGPQYLDG
                     ILDSGVSGNGDEGVMIDAGDAEADPMYDQAVAVVLKTRRPSISLVQRHLRIGYNRAAR
                     LIEQMERAGLVSAMQTNGNRDVLVPAGKGESA"
     gene            3013..>3387
                     /gene="lolA"
                     /locus_tag="VHT22_04870"
     CDS             3013..>3387
                     /gene="lolA"
                     /locus_tag="VHT22_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEX3136917.1"
                     /translation="MIAGWRFAATMCVAMSLACPAAASGPDQLKAFLSGAKTGKTTFR
                     QIVSGKSGRVPQESNGTFAFSRPGKFRWTYEKPHTQIIVGDGQKLWIHDPDLNQVVVR
                     KLDQALGATPAALLAGDDSLEQN"
BASE COUNT          594 a         1044 c         1135 g          614 t
ORIGIN      
        1 gcctcggcgc ggaacttgtt gcggcggtgg accaggctga cctgacgcgc gatgttcgac
       61 agatacagcg cttcttcgac cgcggtgtta ccgccgccga cgacgatgac gtcctgaccc
      121 ttgtagaaaa agccgtcgca ggtcgcgcag gcggaaacgc ctttgcccat gaaagcctgc
      181 tcggaaggca gcccgagata gcgcgccgat gcgccggtcg cgatgatcag cgcgtcgcag
      241 gtgtattcgc cgatgtcgcc gaccaggcgc agcggacgct cgcgcaacgc cgcggtatga
      301 atgtggtcga agatgatctc ggtgtcgaag cgttcggcgt gtttctgaaa gcgcgccatc
      361 agctcggggc cttgcacgcc gtcggaatcg gccggccagt tgtcgacatc ggtcgtggtc
      421 atcagctgcc cgccttgcgc caatccggtg atcagaaccg gtttcaggtt ggcgcgcgcg
      481 gcgtatactg ccgcggtgaa tccggccggg ccggagccga gaatcaacag acgatggtgc
      541 ttggctgaca tgatgcggtc gattcgctgt ttattcgggt acgaaacgtt ccgcccgatt
      601 ctaaccgatg ttggggtggc tccctgaggc gcaagccggc ccttccgcga cgccggacgg
      661 tgtataatgt gttgcgcaaa gtgcgcatca caaatctatg attttcaagc aaaaaataca
      721 aaggcaacgt cgggccgtcg cagcgccgga accgacgctg tcgccgcgcc tcgcggcgct
      781 ggttcgcgag tcgtggtggc tgctggtagt cgccgtattc gcctaccttg cgctgattct
      841 cgcgacctat caacgcaccg atccgggctg gtcattctcg ggcaccggtg cggccattca
      901 caacaagggc ggagcggtcg gcgcgtggct tgccgatctg ctgctgtatc tattcggcat
      961 ctccgcctgg tggtgggtgt tcgccggcat cgtgctggtc gtgaccggct atcgacatct
     1021 gacacgctcg atcgatgctg ccgaccggca tcatccgtgg ctcgccgtgc ctggattcgc
     1081 tctggtcctg ctctcgagct ccgcgctcga ggcactgcgc ctgtatcgcc tccccgcatt
     1141 attgccgcat gcgccaggcg gcgcgctagg cgagatgatc gggtcgggct tctcgcgctc
     1201 tctcggattc aacggcggca cgctgctgct gatcgcactc tttgccatcg gctggtcgtt
     1261 gttcaccggc atgtcgtggc tgcgcctgat ggagcgggtc ggcgccggca tcgagcgcct
     1321 gatcgcgtcg ctgcggcagc gacgcgatgc gcgacgcgat cgtgaaatcg gcgcggtggc
     1381 gctggaggaa cgcgagcagt tcgtcgaggc ggcgcgcgaa agcatcgagg atcgcgaacc
     1441 ggtcgtcgtc gtgccggcga tggtcgacgt gccgaaatcg gaacgggtgg tcaaggaaaa
     1501 gcagaagccg ctgttccagg acatgcccga ctcgccgctg ccgccgctgg cgctgctcga
     1561 ggacgcgcct tcggcgcagg aacaggtcag cccggagacg ctggaattca cctcgcgcct
     1621 gatcgagcgc aagctcgccg atttcggcgt gtcggtgaag gtgctcgcgg cgtaccccgg
     1681 accggtgatc acgcggttcg aaatcgagcc cgcgatcggc gtcaagggcg cacaaatcgt
     1741 caacctgatg aaggatctgg cgcgggcgct gtcggtggtg agcatacgtg tggtcgagac
     1801 cattccgggc aaatcgttca tggggctcga gctgccgaac gccaagcggc agatcgtgcg
     1861 cctcgtcgag ctcttgtcgt cgacgactta caacgatacg gcgagcccgc tgtcgctcgc
     1921 cctgggcaag gatatcgcgg gcaagccggt catcgtcgac ctggcgcgca tgccgcacct
     1981 gctggtcgcc ggcacgaccg gatcgggcaa gtcggtggcc gtcaacgcga tgattctgtc
     2041 gctgctctac aaggccgagc cgcggcaggt ccggttgatt ctgatcgatc cgaagatgct
     2101 ggagctttcg gtttacgaag ggattccgca tctgcttgcg ccggtcgtca ccgacatgaa
     2161 gcttgccgcc aacgcgctgc actggtgcgt cggcgagatg gagcgccgct accggctgat
     2221 gtcgacgctg ggcgtgcgca atctctccgg ctacaacgcg cgcgtggtcg aggccaaaaa
     2281 ggccggcgag ccattgacca atccgttcac gctgacgccg gaaagcccgg agccaatcga
     2341 ggaaatgccg ctgatcgtcg tcgtcatcga tgagctggcc gacctgatga tggtcaccgg
     2401 caagaagatc gaagagctga tcgcccgcat cgcgcaaaaa gcgcgcgccg ccggcattca
     2461 tctgattctt gcgacgcagc gcccgtcggt tgacgtgatc accggcctga tcaaggccaa
     2521 tattccgtcg cgcatcgcgt tccaggtggc gagcaaggta gattcgcgca cgatcctcga
     2581 tcagatgggc gcggagacct tgctcgggca gggcgacatg ctttatctgc cgccgggcac
     2641 cggacacccg cagcgggtgc atggtgcgtt cgtcgccgac accgaggtgc atcgcgtcgt
     2701 cgagcatctg aaagcgcagg gcggaccgca atatctggac ggcattctcg attcgggcgt
     2761 cagcggcaac ggcgacgaag gcgtgatgat cgatgccggc gatgccgaag cggacccgat
     2821 gtacgaccag gcggtcgccg tcgtgctgaa gacgcgtcgg ccatcgatct cgctggtgca
     2881 gcgccatctg cgcattggct acaatcgggc ggcgcgcctg atcgagcaga tggagcgggc
     2941 ggggctggtg tctgcaatgc agaccaacgg caatcgcgac gtcctggtcc cggcgggcaa
     3001 aggcgagagc gcatgatcgc cggatggcgg ttcgcggcga cgatgtgcgt ggcgatgtcg
     3061 ctcgcttgtc ccgcggcagc gtccggcccc gatcaactga aggctttcct gagtggcgcc
     3121 aaaaccggca agacgacgtt tcgccagatc gtcagcggaa aatcggggcg cgtgccgcag
     3181 gaatcgaacg gaacgttcgc attctcgcgg cccggcaagt tccgctggac ctacgaaaag
     3241 ccgcatacgc agatcatcgt cggcgacggc cagaagctgt ggatccacga tccggatctc
     3301 aaccaggtag tcgtcagaaa gctcgaccag gcgcttggcg cgacgccagc cgcgctgctc
     3361 gccggcgacg actcgctgga acaaaac
//
LOCUS       DATANS010000336         3629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_648222, whole genome shotgun sequence.
ACCESSION   DATANS010000336 DATANS010000000
VERSION     DATANS010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648222"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VHT22_04875"
     CDS             complement(<1..552)
                     /locus_tag="VHT22_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3136918.1"
                     /translation="MSLRPADTRVIPLPERLAAVVDTLGSIVVGKQMPLKLALTCLLA
                     RGHLLIEDIPGVGKSTLAQALAATLGLQYARVQFTSDLLPADVLGVSIFDPSTHAFRF
                     HSGPIFHSIVLADEINRAPPKTQSALLEAMEERQVSIDGNTRPLPEPFFVIATQNPAE
                     QIGAYPLPESQLDRFLMTIELGYP"
     gene            694..1623
                     /locus_tag="VHT22_04880"
     CDS             694..1623
                     /locus_tag="VHT22_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX3136919.1"
                     /translation="MSTAYITHPSCRLHAMGPYHPECPERLDAVQDRLISSGLDALIE
                     HHLAPAVTREQLSRVHAAHYIDEIEEASPESGVHYLDPDTALNPDSLDAARHGAGALI
                     LATDLVIGGKRDSAFCAVRPPGHHATRDRAMGFCLFNNVAVGVAHALEHHGLARAAVV
                     DFDVHHGNGTEDIFAGDDRVVMTGTFQYPLYPYSGVEPLGPNMHNVPLSPGSGSDAFR
                     KAFVEVCLPALEAHRPEIIFVSAGFDAHREDPLANLQLTDDDYAWVTSRIAEVAARHA
                     GGRIVSTLEGGYALSALGRSATAHVRVLAGLGP"
     gene            1712..2416
                     /locus_tag="VHT22_04885"
     CDS             1712..2416
                     /locus_tag="VHT22_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136920.1"
                     /translation="MERRQFLRVCTMAAGAAQLGSLGDAWAGAEPRLYGRSKLVDVHG
                     APIRLGDLALKTNYVFNYPYAGTPCFLLNLGRPVAAADNVLRADGASYAWTGGVGPAH
                     AVVSYSAICAHKLAYPTRDISFIRFQSERSPTSDGNVIHCCADHSVYDPAAGARVVSG
                     PAPQPLAAILLDYDGATDGVAALGTVGAEQFDAFFRKYDFKLAMEYGQGKARMPVGAT
                     TVVRELTQYCRQTIQC"
     gene            complement(2433..3038)
                     /locus_tag="VHT22_04890"
     CDS             complement(2433..3038)
                     /locus_tag="VHT22_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX3136921.1"
                     /translation="MIKVYGTSLSGNCHKVRMVLEALHLPYTWTEIDSSKGETRTPEF
                     LARNPNGKVPVVELEPGVYLPESNAIICYLADASPLMPSDRLLRARVLSWLFFEQYSH
                     EPYIAVARFLCRFHADPPSQRALLESKMPGGYRALEVLEKALADAPFLVGGRYTLADI
                     ALYAYTHVADEGGFDLARFPAIRSWLSRVAAQPGYVPMVAP"
     gene            3158..>3629
                     /locus_tag="VHT22_04895"
     CDS             3158..>3629
                     /locus_tag="VHT22_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136922.1"
                     /translation="MTTRIAVLGLYRSGSSAAAGVLHHLGVEMGAPYWMDHYESARLA
                     KRLRSWWHEPHLEERFPPAERVRKLSDWISFLEAGRATHVGAKHPLLSLCGDDLVEAW
                     GPAVRFIWTWRPLDESIASIRRTDWWWAKPKGETVQRRLWDAVNRFFASREHLRV"
BASE COUNT          605 a         1182 c         1224 g          618 t
ORIGIN      
        1 gggatacccg agctcgatcg tcatcaggaa acggtcgagc tgcgattcgg gcagcgggta
       61 tgcgccgatc tgttccgccg gattctgcgt cgcgatcacg aagaacggct cgggcagcgg
      121 ccgcgtgttg ccgtcgatcg agacctgccg ctcctccatc gcttcgagca gagcgctttg
      181 cgtcttcggc ggcgcgcggt tgatttcgtc ggccagcacg atcgagtgga aaatcggtcc
      241 cgaatgaaaa cggaaggcgt gggtcgaggg atcgaagatc gacacgccga gcacgtcggc
      301 gggcaaaaga tcgctggtga actggacgcg cgcatactgc agccccagcg tcgccgccaa
      361 ggcctgcgcc agcgtcgact tgccgacgcc cggaatgtcc tcgatcagca ggtggccgcg
      421 cgccagcagg cacgtcaatg cgagcttgag cggcatctgc ttgccgacga cgatgctgcc
      481 gagcgtatcg acgactgccg ccagcctttc gggcagcgga atgacgcggg tatcagcggg
      541 gcgcaggctc attgattctt cgcgggtatt ggccagacga gcactataga gggcaatcgg
      601 cggctagtca ttaccgctcg cggcgcgcta cactccgctc gacccccaag ccgcggcggc
      661 gcgtgttgcg cccgtggccg accgaccgaa gccgtgtcca ccgcgtacat cacccatccc
      721 tcgtgccgtc tgcatgcgat gggaccgtac caccccgaat gcccggagcg gctcgatgcc
      781 gtgcaggatc ggctgatcag ctcgggactc gacgcgctga tcgagcatca tctcgcgccg
      841 gcggtgacgc gcgagcaact ctcgcgtgta catgctgcgc actacatcga cgagatcgag
      901 gaggcgagcc ccgaaagcgg cgtgcattac ctcgacccgg acaccgcgct caatcccgac
      961 tcgctcgacg ccgcccggca tggcgctggg gcactgatac tcgcgaccga tctcgtcatc
     1021 ggcggaaagc gcgactcggc gttctgcgcc gtgcgcccgc ccggccatca tgcgacgcgc
     1081 gatcgggcga tgggcttctg cctgttcaac aatgttgcgg tcggcgttgc gcatgcgctc
     1141 gagcaccacg gtctcgcccg cgccgcagtc gtcgatttcg acgtgcatca tggcaatggc
     1201 accgaggata tctttgccgg cgacgatcgc gtggtgatga ccggaacgtt ccagtacccg
     1261 ctgtatccgt attcgggcgt cgagccgctg ggcccgaaca tgcacaacgt tcccctttcg
     1321 cccggcagcg gcagcgatgc ttttcgcaag gcctttgtcg aggtgtgcct gccggcgctc
     1381 gaggcgcatc ggccggagat cattttcgtg tcggccggat tcgacgcgca ccgcgaggat
     1441 ccgctggcca acctgcagct gaccgacgac gactacgcat gggtgacgag caggatcgcc
     1501 gaggtcgcgg cccggcacgc cggcggtcgc atcgtatcga cgctcgaagg cggctacgcg
     1561 ctgagtgcgc tcggccgcag cgcgaccgcg cacgtgcgcg tgctcgccgg cctgggtccg
     1621 tagccgggcg cgacagcctt ggtccgcgcc ggcgaccggg cgggcttttg cgcgttgcgt
     1681 tgtcgcgcgc aagcgaggag gtagagcaac gatggaacga cgacaattct tgcgcgtctg
     1741 tacgatggcc gccggcgccg cgcagctggg ttcgctcggc gatgcctggg ccggcgccga
     1801 gccgcgcttg tacggacgct cgaagctggt cgatgttcac ggcgcgccga tcagactcgg
     1861 agacctcgcg ctaaagacca actacgtatt caactaccct tatgcgggca cgccgtgctt
     1921 cctgctcaac ctcgggcggc ctgtagctgc cgccgacaac gtcttgcgcg ccgacggcgc
     1981 gagctacgcg tggaccggtg gcgtcggccc cgcacacgcg gtggtttcgt actcggcgat
     2041 atgcgcgcac aagctcgctt atccgacgcg ggacatctcg ttcatccggt ttcaatcgga
     2101 gcgctcgccg acctccgacg gcaacgtgat tcattgctgc gccgaccata gcgtctacga
     2161 tcccgccgcg ggcgcgcgcg tggtcagcgg gcccgcaccg cagccgcttg cggcgatcct
     2221 gctcgactac gacggcgcga ccgacggcgt cgcggcgctg ggcaccgtcg gcgccgagca
     2281 gttcgatgcg ttcttccgga aatacgactt caagctcgcg atggagtacg gccagggcaa
     2341 ggcccgcatg ccggtcgggg cgacgacggt cgtgcgcgag cttacccagt actgccggca
     2401 gacgatccag tgctgagcgc cgcgccgcgg cgtcagggtg cgaccatagg aacgtaaccg
     2461 ggctgcgcgg cgacgcgcga aagccagctg cggatcgcgg gaaaccgggc gaggtcgaag
     2521 ccgccctcgt cggcgacatg ggtgtacgcg tacaaggcga tgtcggcgag cgtgtagcgt
     2581 ccgccgacca ggaacggcgc atcggccagc gccttctcga gcacttccag cgcgcgatag
     2641 ccgccgggca tcttggattc gagcagcgca cgctgcgatg gcgggtccgc atgaaagcgg
     2701 cacagaaagc gcgcgacggc gatatacggt tcgtggctgt actgctcgaa aaagagccac
     2761 gagagaacgc gggcccgcag caatcgatcg gagggcatca gcggcgacgc gtcggcgaga
     2821 tagcagatga tcgcgttcga ttccggcaga tacacgccgg gctcgagctc gaccactggc
     2881 accttgccgt tcggattcct ggcgagaaac tcgggcgtgc gcgtctcgcc tttcgaggag
     2941 tcgatttcgg tccaggtata gggcagatgc agggcctcga ggaccatgcg gaccttgtgg
     3001 cagttgccgg acaaagacgt gccgtagact ttgatcatcg ctgtgtctct tcgctgatgc
     3061 ggacaggaga gaaatctgca ttacaatcgc gccgggcaca agcttttccg tggaggtttt
     3121 gaagatgcat cacgcttggc gataggcgca atcgccgatg acaacgcgga tagccgtgct
     3181 cggcttgtat cgctccggct cgtcggccgc cgcgggtgtg ctgcatcacc tgggcgtcga
     3241 gatgggtgcg ccgtactgga tggaccatta cgaatcggcg cgccttgcga agcgactccg
     3301 gagctggtgg cacgagcccc atctggagga gcgctttccc cccgccgaac gcgtgcgcaa
     3361 gctcagcgac tggatctcgt tcctcgaagc gggccgcgcg acccacgtcg gcgccaagca
     3421 cccgctgctc tcgctttgcg gcgacgacct ggtcgaagcc tggggtccgg cggtccgctt
     3481 tatctggacc tggcgccctc tggacgaatc gatcgcgtcg attcgccgca ccgactggtg
     3541 gtgggccaag cccaagggcg agacggtgca gcgtcgtctt tgggatgccg tcaaccggtt
     3601 tttcgccagc cgcgagcatc ttcgcgtcg
//
LOCUS       DATANS010000337         3122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_395593, whole genome shotgun sequence.
ACCESSION   DATANS010000337 DATANS010000000
VERSION     DATANS010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395593"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="VHT22_04900"
     CDS             <1..129
                     /locus_tag="VHT22_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HEX3136923.1"
                     /translation="MQELNREANTLGSKSVDAELSHAALELKVLIEQMREQVQNIE"
     gene            160..777
                     /gene="gmk"
                     /locus_tag="VHT22_04905"
     CDS             160..777
                     /gene="gmk"
                     /locus_tag="VHT22_04905"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX3136924.1"
                     /translation="MTTPTGSLFVVAAPSGGGKTSLTRALLDKDRGIRLSVSYTTRPP
                     RLGEREGVDYHFVSIEEFMALRDAGEFLEHAHVHGNWYATSASWLNQQIEAGQDVLLE
                     IDWQGAAQVRRLIPSSIQVFILPPSLALLEERLHRRGQDDKATIARRLEAARDEIRHC
                     AEFNYVIINQDFATAVDDLSAIVRASRLRAAQQCVRHEALIAQLI"
     gene            819..1025
                     /gene="rpoZ"
                     /locus_tag="VHT22_04910"
     CDS             819..1025
                     /gene="rpoZ"
                     /locus_tag="VHT22_04910"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX3136925.1"
                     /translation="MARITIEDCLVRIPNRFELTLAATNRARQISAGSQPLVESDRDK
                     TTVIALREIAAGKVGIEMLLKPQS"
     gene            1062..>3122
                     /locus_tag="VHT22_04915"
     CDS             1062..>3122
                     /locus_tag="VHT22_04915"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX3136926.1"
                     /translation="MSEIAEFTRHLGHYLQPPDVALVERAFAFSEQAHQGQFRKSGEP
                     YITHPLAVASILSQWRLDAQGLAAALLHDVMEDSGITKNEIETSFGKPVAEMVDGVSK
                     LDQIEFQSREDAQAENFRKMLLAMAQDVRVILIKLADRLHNMRTLDAMAPTHRERIAR
                     ETLDIYAPIANRLGLNALYLELQELSFKHLHPARYRVLAKAIKAARGNRREVMNRILQ
                     SIRDGLKEADIAATVTGREKTIYSVYKKMREKRYTFSQVFDIYGVRVLVRDTTACYAA
                     LGVLHNLYKPIPGKFKDYIAIPKANAYQSLHTTLFGPFGTPLEAQIRTQDMHRIAESG
                     VAAHWLYKAGGELDIAEAQEQTHRWLQSLLEIQSESRDSKEFLEHIKGDLFPDAIYVF
                     TPKGKIMALPHGATAVDFAYAVHTDIGHHCTAARINYELMPLRTELKNGDQVEIITSP
                     SARPNPSWLGFVATGKARSRIRHFLKSLQQRESALLGERMLEQALISLKVPPASVNWE
                     RWEALAREYGAKGQTEILADIGLGKRLSFVVAQALTRSAGRTSEEPAPMQAKHGALQL
                     RGVEGVAIQYGKCCRPIPGDAIIGHFRRGQGLLVHTRDCVTLRKGRIDSDQVVDVEWA
                     PEIQGVFEAGIRLVVADERGLLAHLATAIADAGANIEYLSMERPDGGHVVAMFFSVQV
                     RDRRH"
BASE COUNT          618 a         1003 c          984 g          517 t
ORIGIN      
        1 atgcaggagc tcaatcgcga agccaacacg ctgggctcca aatcggtcga tgctgagctt
       61 tcgcacgcgg cgctcgagct caaggtgctg atcgagcaga tgcgagagca ggtgcagaac
      121 attgaatagg actctggaac cggaaaggct gctcgaaccg tgacgactcc caccggcagc
      181 ttgttcgtcg tcgcggcgcc ttcaggcggc ggcaagacca gtctgacgcg ggcgctgctg
      241 gacaaagacc gcggcatccg cctttcggtc tcgtacacga cgcgcccgcc gcgtctcggc
      301 gagcgggaag gcgtcgacta ccacttcgtc tcgatcgagg agttcatggc gctgcgcgac
      361 gccggcgagt ttctcgagca cgcccacgtt catggcaact ggtacgcgac gtcggcgagc
      421 tggctcaacc aacagatcga agcgggccag gacgttctcc tggaaatcga ctggcagggc
      481 gcggcgcagg tgcgtcgtct gatcccgtct tcgatccaag ttttcatcct tccgccttca
      541 cttgcattgc tggaagagcg cctgcaccgg cgcggccagg acgacaaggc gacgatagcg
      601 cggcgcctcg aggcggcgag ggacgagatc cgtcattgcg ccgagttcaa ttatgttatt
      661 attaatcagg actttgccac tgcggtcgac gacttgagcg ccatcgtgcg ggcttcgcgc
      721 ctgcgcgcgg cgcaacaatg cgttcgccac gaggcactga ttgcacaact catttagcgt
      781 gcgagcgaat tcccgactcg gcatgcaaga aaggcaccat ggcccggatc acgatcgaag
      841 actgccttgt caggataccc aaccgcttcg agctaaccct cgccgcgacc aatcgcgcac
      901 ggcagatcag cgccggatcg cagccgctgg tcgagagcga ccgcgacaag acaacggtga
      961 ttgcgctgcg cgaaatcgct gccggcaagg tcggcatcga aatgctgctc aagccgcaaa
     1021 gttgacttgc atcgggcggc ttaaccccaa cgtgatcaag catgtcggaa atcgccgaat
     1081 ttacccgaca ccttggccac tacctgcaac cgcctgacgt cgcgctcgta gagcgggcgt
     1141 tcgccttttc cgaacaggcc caccagggac agtttcgcaa gtcgggcgag ccttacatca
     1201 cccacccgct ggcggtagcc agcatactgt cgcagtggcg gctcgacgcc caggggctcg
     1261 ccgccgcgct cctccacgac gtgatggagg acagcggcat caccaagaac gagatcgaga
     1321 cctcgttcgg caagcccgtc gccgagatgg tcgacggcgt gtccaagctc gatcagatcg
     1381 agttccagtc acgcgaggac gcgcaggctg agaatttccg caagatgctg ctggcgatgg
     1441 cgcaggacgt tcgcgtcatc ctgatcaagc tcgccgaccg gctgcacaac atgcgtacgc
     1501 tcgacgcgat ggcaccaacc catcgcgagc ggatcgcccg cgaaacgctg gacatctacg
     1561 cgccgatcgc caatcgcctc ggcctgaatg ctctgtatct ggaactgcag gagctctcgt
     1621 tcaagcatct gcatccggcg cgctaccggg tgctggccaa ggcgatcaag gctgcgcgcg
     1681 gcaaccggcg cgaagtgatg aaccggatcc tgcagtcgat ccgcgatggc ctgaaggagg
     1741 ccgacatcgc ggcgacggtg accggacgag aaaagactat ctacagcgtc tacaagaaaa
     1801 tgcgcgagaa gcgctatacg ttctcgcagg tgttcgacat ttacggcgtg cgcgtcctgg
     1861 tcagagacac gaccgcctgc tatgcggcgc tgggcgtgct gcacaatctt tacaagccca
     1921 tccccggcaa attcaaggat tacatcgcca ttccgaaggc caacgcgtac cagtcgctgc
     1981 atacgacgct gttcggcccc ttcggcacgc cgctggaggc gcagatccga acgcaggaca
     2041 tgcatcgcat cgccgagtcc ggcgttgccg cgcactggct gtacaaggcg ggaggcgagc
     2101 tcgacatcgc cgaggcccag gagcagacgc atcgctggct gcaaagcctg ctcgagatac
     2161 agtccgagtc gcgcgactcg aaggagttcc tcgagcacat caagggcgac ctgtttcccg
     2221 acgcgatcta cgtgtttacg ccgaagggca agatcatggc cctgccccac ggcgcgaccg
     2281 cggtcgactt cgcctacgcg gtgcacaccg atatcgggca ccactgcacg gcggcccgga
     2341 tcaactacga gctcatgccg ctgcgcaccg agctcaagaa cggcgaccag gtcgagatca
     2401 tcacctcgcc cagcgcgcgt ccgaatccgt cgtggctcgg gtttgtcgcc accggcaagg
     2461 cgcgctcccg catccggcac tttctgaagt ccctgcagca gcgcgagtcg gcgctgctcg
     2521 gcgagcggat gctggagcag gcgctgatct cgctgaaggt tcctcccgcg tcggtaaact
     2581 gggagcgctg ggaagcgctg gcgcgcgagt acggcgccaa ggggcagacc gagattcttg
     2641 ccgatatcgg cctgggcaag cggctttcat ttgtcgtcgc acaggcgctg actcgcagcg
     2701 ccggccggac cagcgaggag ccggctccca tgcaggccaa gcatggcgcg ctgcagctgc
     2761 gcggcgtcga aggcgtcgcg atccagtacg gcaaatgctg ccggccgatt cccggagacg
     2821 cgatcatcgg ccatttccgc cgcggccagg gtctgctggt gcacacccgc gattgcgtga
     2881 cgctcaggaa agggcgaatc gacagcgatc aggtcgtcga cgtcgaatgg gcgccggaga
     2941 tccagggcgt attcgaagcc gggatccggc tggtggtggc cgacgagcgc ggcctgctcg
     3001 cgcacctggc gacggcgatc gccgacgccg gagcgaatat cgaatatctg tcgatggaac
     3061 gacctgacgg cggccacgtc gtcgcaatgt tcttcagcgt ccaggtgcga gaccgaaggc
     3121 at
//
LOCUS       DATANS010000338         2690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_269681, whole genome shotgun sequence.
ACCESSION   DATANS010000338 DATANS010000000
VERSION     DATANS010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269681"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1597
                     /gene="pcaB"
                     /locus_tag="VHT22_04920"
     CDS             245..1597
                     /gene="pcaB"
                     /locus_tag="VHT22_04920"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HEX3136927.1"
                     /translation="MPSTLIDSTILGNLVGTEAMRALFSDDNRTQRYLDVEAALARVQ
                     GQLGVIPKAAAAEIERNCRVDRIDMAKLRAQTERAGSPIVGVVQQLGALCAEPHGRYC
                     HWGATTQDITDTATVLQMRDALALIDIELAAVADALAGLALRYRDTPMAGRSHLQQAA
                     PISFGYKAASLLSAIERHRARLAELKPRVLVGEFGGAVGTLAALGPEGLEVRSALMHE
                     LGLAEPDISWHTQRDRIAEAGCFLGLVTGTLAKLAIDVKLMMQTEVGEVAEPFTPGRG
                     ASSTMPQKRNPVSSVYLHACAAVVRQHAALLLEAMAADHERATGPWHIEWIVLPEAFV
                     LTAGALVHARTIVDGLQVDAARMRGNLDLTHGLIVAEAVMMGLAPHLGRERAHDLVYE
                     LCRMAIERQRPLLDLLAETAEVTAHLDRQRLAALCEPANYLGFAGEMVDRVLAARARH
                     "
     gene            complement(1632..>2690)
                     /locus_tag="VHT22_04925"
     CDS             complement(1632..>2690)
                     /locus_tag="VHT22_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX3136928.1"
                     /translation="YPVQLALLPAVAALAAGNRALIKPSEITPCASALLASLVARYFS
                     LEELAVVNGGADVGAAFARLPFDHLLFTGSTQVGRSIALAAAENLTPVTLELGGKSPA
                     LIDGRADLSALSARLAVGKLLNAGQTCIAPDYVLIPRQRAEALTQALQRAVARLYPAP
                     VDNRDYASIIDERHYSRLAALLDDARAKGARVIELGASSGSAAGGRRRMPPALVLDVS
                     DTMGVMQQEIFGPILPIETYDTLDQAIERINARPRPLAMYMFGGSAAERRRVLERTIA
                     GGVTLDDTLWHFTNENLPFGGVGASGYGAYHGERGFLTFSHQKAVFAQSRATPGRLLY
                     PPYGERFEATLRLLKRIV"
BASE COUNT          441 a          906 c          918 g          425 t
ORIGIN      
        1 gacgtaggct cgccgtcggg cccgataccg gcgctgcttc cgccgggaac cgatgacgag
       61 gccgatccgc agtggagcgc cgtgccggcg ctgggtgcgc ataccgacag catcctgacg
      121 gcgctcggct atccggtcga acggatcgcc gaactccgcg ccgacggcgc gatatgatct
      181 cccgcatgct gcgcggccgg agcggttgcc gtcctcgcgc cgatctcgcc ttcggagcaa
      241 aacgatgccc tcgaccctga tcgactcgac gatcctcggc aacctggtcg ggaccgaagc
      301 aatgcgtgcg ctgttttccg acgacaatcg cacgcaacgc tatctcgatg tggaggccgc
      361 tcttgcgcgc gtgcaggggc aattgggtgt cattccgaag gcggcggccg ccgaaatcga
      421 gcgcaactgc cgcgtcgacc gtatcgacat ggcgaagctt cgcgcgcaaa ccgagcgcgc
      481 cggctcgccg atcgtcggcg tggtgcagca gcttggcgcg ctgtgcgccg agccgcatgg
      541 tcgctactgt cactggggcg cgaccacaca ggacatcacc gacaccgcga cggtgctgca
      601 gatgcgcgac gcactcgcgc tgatcgacat cgaactcgcc gccgtcgccg atgcgctggc
      661 cgggctggcg ctgcgctacc gcgatacgcc gatggctggg cgcagccatc tgcagcaggc
      721 ggcgccgatc agcttcggct acaaggctgc cagcctcctt tccgcgatcg agcgccatcg
      781 cgcgcggctc gccgagctta agccgcgcgt cctggtcggc gaattcggcg gcgcagtcgg
      841 cacgcttgcc gcgctcggac cggaggggct cgaggtccgc agtgcgctga tgcacgaatt
      901 gggactcgcg gagcccgata tcagctggca tacgcagcgc gaccgcatcg ccgaggccgg
      961 ttgctttctc ggcctggtca ccggcacgct cgccaagctc gccatcgacg tcaagctgat
     1021 gatgcagacc gaggtcggcg aggttgccga gccgtttacg ccgggtcgcg gcgccagcag
     1081 cacaatgccg caaaagcgca acccggtttc cagcgtgtac ttgcacgcct gcgccgcggt
     1141 cgtgcgccag catgctgcgc tgctgctcga ggcgatggcg gcggatcacg agcgcgccac
     1201 cggtccctgg catatcgaat ggatcgtgct gccggaagca ttcgtgctga ccgccggcgc
     1261 actggtgcac gcgcgaacga ttgtcgacgg tctgcaagtc gacgccgcgc ggatgcgcgg
     1321 caacctcgat ctgacgcatg gactgatcgt cgccgaggca gtgatgatgg ggctggcgcc
     1381 gcatctgggt cgcgaacgcg cgcacgacct ggtctacgag ctttgccgga tggcgatcga
     1441 gcggcagcgt ccgctgctgg acctcttggc cgagactgcc gaggtcaccg cgcatctgga
     1501 tcgacagcgt ctggccgcac tgtgcgagcc cgcgaactac ctgggatttg ccggcgagat
     1561 ggtcgatcgc gtgcttgccg cccgcgcccg gcactgaatg cgccgccgga tgggcggcgc
     1621 aatggtcccc cctacacgat ccgcttgagc aggcgcaaag tcgcctcgaa acgctcgccg
     1681 tagggcggat acaagagtcg gcccggtgtg gcgcgcgatt gcgcgaagac ggccttctga
     1741 tgggaaaacg tcaggaatcc gcgctcgccg tgatacgcgc cgtaaccgga tgctccgacg
     1801 ccgccgaacg gcaggttctc attggtgaaa tgccagaggg tgtcgtccag cgtcacgccg
     1861 ccggcgatcg tccgctccag aacccgacgt cgctcggctg cgcttccgcc aaacatgtac
     1921 atcgccagcg gccgcggtcg cgcgttgatc ctctcgatcg cctggtcgag tgtgtcgtaa
     1981 gtctcgatcg gaagtatcgg accgaaaatc tcctgctgca tgacgcccat cgtgtcgctg
     2041 acgtcgagca ccagcgccgg cggcatgcgg cgccgtccac ccgccgcgga gccgctcgat
     2101 gcaccaagct cgatcactcg cgcacccttg gcccgcgcgt cgtccaggag cgcggcgagt
     2161 cgtgaatagt gccgctcatc gatgatgctg gcgtagtccc gattatcgac cggcgccgga
     2221 tacagtcggg ccaccgcacg ctggagcgcc tgggtgagcg cttcggcgcg ctggcgggga
     2281 atcagcacat agtcgggcgc gatgcacgtc tgtccggcgt tgagcagctt gccgacggcc
     2341 aggcgcgccg aaagcgcgga gaggtcggca cggccatcga tcagcgcggg cgattttccg
     2401 ccgagctcga gcgtcacggg cgtcagattt tccgcggcgg cgagcgcaat gctgcgtccg
     2461 acctgggtcg atccggtgaa caatagatgg tcgaacggca ggcgggcgaa cgcggcaccg
     2521 acgtccgcac cgccgttgac cacggcgagc tcctccagtg aaaagtagcg cgcgaccagc
     2581 gaggccaaga gcgccgacgc gcacggagtg atttcgctcg gcttgatcag cgcgcgattg
     2641 cccgcagcga gcgcggcgac cgccggaagc agagcgagct gtaccgggta
//
LOCUS       DATANS010000339         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_144085, whole genome shotgun sequence.
ACCESSION   DATANS010000339 DATANS010000000
VERSION     DATANS010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144085"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1471
                     /locus_tag="VHT22_04930"
     CDS             <1..1471
                     /locus_tag="VHT22_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3136929.1"
                     /translation="PPAERAQLLEQLSYECYLIDQVQRAIDSRREALEIWRAQGARKQ
                     EGDALRWLSRLSWLEGRRAEADEYGAAAVAALESLPPGPELAMAYSNRAQLDMLAADA
                     DSAEQWSQRTLQLAEQAGYDEISCHALNNLGTARLIAGNDAGWDDLNRSLQLALAKGF
                     QEHAARAYTNLAAMAIQRKQYTAGSRYLSEGLTYCERHDLDSWRFYGMAWRGRARFEQ
                     GDWLNAAEDAQAIVSEAGSAPIARIPALTILAHLRIRRGDPDVTTPFEEARTLAARTM
                     EMQRLCGVAVAAADEAWISGDRQRIAQEVQPVYDLASTRRDLWMKGELALWLWRAGAF
                     ATPPTDIAEPYALEISGDWRGAAQAWQALGCPYEQANALASGGGEQQQLEALTIMERL
                     GATAAANALRRRMREQGMRRIPRGSRTSTRTNTHGLTKREAEIFELLAQGLRNSAIAK
                     RLFVTTKTVDHHVSAIFTKLGVSSRAEAIALSKKPAESG"
BASE COUNT          297 a          495 c          533 g          267 t
ORIGIN      
        1 tcctcccgcg gaacgtgcgc agttgctcga gcagttgtcg tacgagtgtt acttgatcga
       61 tcaggtgcag cgcgcgatcg actcgcggcg cgaagcgctc gagatctggc gtgcgcaggg
      121 cgcgcgcaaa caggaaggcg acgcgttgcg atggctgtcg cgcctgagct ggctggaagg
      181 tcgccgcgcg gaagctgacg aatatggtgc tgcagctgtc gctgcgctcg agtcgctgcc
      241 gccaggtccg gagctcgcga tggcatacag caatcgcgcg caactcgaca tgctggcggc
      301 agacgccgac tcggccgagc aatggtcgca gcggacgctg cagctcgcgg agcaagcggg
      361 ttacgacgaa atttcgtgcc acgctctcaa caatctcggc accgcgcgtc tgatcgccgg
      421 caacgatgca ggctgggatg atttgaatcg cagcctgcag ctggcgctgg cgaaaggttt
      481 tcaggagcac gcggcacgcg catataccaa cctcgcggca atggccatcc agcgcaagca
      541 atacaccgcc gggtcgcgct atctcagcga aggcctgacg tattgcgagc gacacgacct
      601 cgactcgtgg cgcttctatg ggatggcgtg gcgcggtcgc gcgcgcttcg agcagggcga
      661 ctggctcaac gccgctgagg atgcacaggc aatcgtctcc gaagctggca gcgcaccaat
      721 cgcgcgtatt cctgcgctca cgattctcgc gcacctgcgc attcgacgcg gtgacccgga
      781 cgtgactacg ccgttcgaag aagctcgcac gctcgccgcg cggacgatgg aaatgcagcg
      841 gctttgcgga gtcgcggtgg ccgctgcgga cgaagcctgg atttccggcg accgtcaacg
      901 catcgcacag gaagtgcagc ccgtgtacga cctcgcgtct acacgtcgcg atctgtggat
      961 gaaaggcgag ctggcgttgt ggctgtggcg cgcgggtgca ttcgcgacgc cgccgactga
     1021 tatcgccgag ccctatgcgc tggaaatttc cggcgactgg cgtggtgccg cgcaggcctg
     1081 gcaggcgctc ggctgtccgt acgagcaggc gaatgcgctg gcatcgggcg gcggcgagca
     1141 gcaacaactg gaagcgctca cgatcatgga gcggcttggc gcaacggctg cggccaatgc
     1201 attgcgtcga cggatgcgcg agcagggcat gcggcgcatc ccgcgcggct cacgcacttc
     1261 gactcgcacg aatacgcatg gcttgaccaa gcgcgaagcg gagattttcg agctgctggc
     1321 gcagggcctg cgcaactcgg cgatcgcgaa gcggctgttc gtcacgacca agacagtcga
     1381 tcatcacgtc tcggcgatct tcacgaagct cggtgtgtcc tcgcgcgccg aagcgatcgc
     1441 gctcagcaag aagccggcgg agtcaggctg aagcctgacc cacagccaga aaaaagcccg
     1501 acccacattg tctgtgggtc aggctttagc ctgacatctg ttacttcggc agcaacgtga
     1561 ccggcacttc catgacgttg tcgaccggca gc
//
LOCUS       DATANS010000340         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_312476, whole genome shotgun sequence.
ACCESSION   DATANS010000340 DATANS010000000
VERSION     DATANS010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312476"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1397
                     /locus_tag="VHT22_04935"
     CDS             <1..1397
                     /locus_tag="VHT22_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3136930.1"
                     /translation="LPAMSAATKERREPVTIVGAGLAGTLVATLLARRGHPVRIFERM
                     PDMRHSTIPAGRSINLALAARGIRALELAGVMDRVRPLLIPMPGRMLHAIDGALTFVP
                     YGQGEHEVIYSVSRPDLNRILIDAAERAGVEIRFRTAAVGADIRHHALVLRDEVASRL
                     EECPLRRVIAADGAGSVVRRALVSELEVPASEDLLKHGYKELSLPPGAGGTHRIEKHA
                     LHVWPRGGFMLIALPNLDGSFTVTLFLPHQGEESFASLTEPSRITQFFRRHFPDICAM
                     LPALTDEFLKHPTGIMGTVRCERWSVDDRLLLIGDAAHAITPFHGQGMNCAFEDCREL
                     VAMLDTTTDWSSTFRRFEAQRRPHTDAIADMAIENYLEMRDTVRDPQFHLQKTLSLEL
                     ERRFPQRFVPRYSMVMFHDEIPYAVALERGRIQNKILQQLTRNVSTLGAVDFQAAQRM
                     IEERLTPISPQAPK"
BASE COUNT          263 a          521 c          476 g          258 t
ORIGIN      
        1 cgcttcctgc gatgagtgcc gcgaccaaag agcgccgcga gccggtaacg atcgtcggcg
       61 caggcctcgc ggggacgctg gtcgcaacgc tgctcgcgcg gcgcgggcac ccggtccgca
      121 tcttcgagcg gatgccggac atgcggcact cgacgattcc cgccggccgt tcgatcaatc
      181 tcgcgctcgc ggcacgcggc atccgtgcgc tcgagctcgc cggcgtgatg gatcgcgtgc
      241 gcccgctgct cattccgatg cccggccgca tgctccacgc gatcgacggc gcgctcacgt
      301 tcgtgccgta cggccagggc gagcatgaag tgatctactc cgtctcgcgc cccgatctca
      361 atcgcattct gatcgacgcc gcggaacgcg ccggagtcga gatcagattc cggacagcgg
      421 cagtcggcgc ggatattcgc catcacgccc tggtgttgcg cgacgaggtc gcgagccggc
      481 tcgaggaatg ccctctgcga cgcgtgattg cggccgatgg cgccggctca gtcgtgcgac
      541 gcgcgctggt aagcgagctc gaagtgcccg cctccgaaga tctgctcaag catggctaca
      601 aggagttgag tctgccgccc ggcgccggtg gcactcatcg catcgagaaa cacgcgctgc
      661 acgtctggcc gcgcggcggc ttcatgctga tcgcgttgcc gaatctcgat ggcagcttca
      721 cggtcacgct gttcctgccg caccagggcg aagagagctt cgcatcgctc accgagccca
      781 gccgcatcac gcagttcttc cgccgccatt ttccggacat ctgcgcgatg ttgccggcgc
      841 tcaccgatga gttcctgaag catcccaccg gcatcatggg taccgtgcgc tgcgagcgct
      901 ggtcggtcga cgaccggctg ctgctgatcg gcgacgccgc gcacgcgatt acgccgttcc
      961 acggtcaagg aatgaactgc gcgttcgaag actgtcgcga gctggtggca atgctcgaca
     1021 cgacaaccga ctggagcagc accttccgcc gcttcgaagc gcagcggcgg ccgcacacgg
     1081 atgcgatcgc cgacatggcg atcgagaact atctcgagat gcgcgacacc gtgcgcgatc
     1141 cgcaattcca cctgcagaag acgctgtcgc tggagctgga gcggcgtttc ccgcaacgct
     1201 tcgtgccgcg ctattcgatg gtgatgtttc acgacgaaat cccgtacgcc gtcgcactgg
     1261 agcgcggccg catccagaac aagatcctgc agcagctcac gcgcaacgtc agcacgctcg
     1321 gcgcagtcga tttccaggct gcgcagcgca tgatcgagga acggctgacg ccgattagcc
     1381 cgcaagcccc aaaataacgg ctgtgctgtc cgcggtttgc cggactgtgc cttggcgcga
     1441 tagcccccct caatagaatg gcccgcagct ccaacggaga agccgccatg cgcgaactcg
     1501 cctttcgcat cgtcaacg
//
LOCUS       DATANS010000341         2529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_602773, whole genome shotgun sequence.
ACCESSION   DATANS010000341 DATANS010000000
VERSION     DATANS010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602773"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1972)
                     /locus_tag="VHT22_04940"
     CDS             complement(<1..1972)
                     /locus_tag="VHT22_04940"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3136931.1"
                     /translation="MSAIASEPPRNAPCPCGSGKRYKDCHGALQPPLAAGGVAPLLAK
                     ARSALALGDAGQAEAAWRQALDADPDEAEALFHLGNLERERGQHDLAVGHYRRALLRA
                     PGHAGVLNNLGLTYEAQGRSAEAEACYRDVLAATPNHPDALANLANIQFGREDFAAAA
                     ESYTRAFAIRRDAPAGIWVRRALAQRRSHDMAGAEASLREAARIAPDDTDVQINLASA
                     CAERYRWAAAEAPLLRALTLDPVNPYALSMLAHARQHRCDWSGLDELFASINRQLEDG
                     NATHRHLANPFPVLAMPTSPRAQLHAAQRWARGFAPSPPLPRPTVTMDNGERLRIGFV
                     SSDLREHPMAHLQVEHWERIDRGRFETCAYSLLPRQESAIGRRVAAAFDRYADVSGDT
                     LTSIAQRIRNDRIAILFDLNGYTTHSREHLFAQRPAPIQINSIGFPGTLGADWYDYIL
                     VDRFAAAEATQPYYTERLWPMPNSFYPSDTTRAPRGTAPSRLACGLPDKGFVFCCFNK
                     AYKILPDVFAIWMRLLDAVPDSVLWLLESGPDATRHLMQEARRAGIDPVRLLFAPHVP
                     VEEHLARHAAADLFVDTFPYGAHTTSNDALLAGLPVVSCAGETLVSRIAGSQLHAIGL
                     PELVTTSLAEYEALALRLAREPETLAALRTRLA"
     gene            complement(1953..>2529)
                     /locus_tag="VHT22_04945"
     CDS             complement(1953..>2529)
                     /locus_tag="VHT22_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136932.1"
                     /translation="QGFVFACFNAPYKILPPVFDVWMRLLREVPGSVLWLSPGYPIAC
                     ANLQREAIARGVDPRRLAFAPRVGLAEHRARHAHADLFLDTLPYNAGTTANDALFMGV
                     PVLTCSGETMASRIAGSQLHAIGLPELVTTSLAEYEALALRLAREPETLAALRTRLAA
                     NRHTHPLFDMARYTQDFEALLLHTWDERHRL"
BASE COUNT          405 a          824 c          905 g          395 t
ORIGIN      
        1 cggcgagcct ggtgcgcagg gccgccaggg tttccggctc gcgggccagg cgcaaggcga
       61 gcgcttcgta ctcggccagg ctcgtcgtca ccagctccgg cagtccgatc gcatgcagct
      121 gactgccggc aatgcggctg accagcgttt ccccggcgca gctgacgaca gggagtccgg
      181 ccagcagcgc atcgttgctc gtcgtatgcg cgccgtacgg aaacgtgtcg acaaaaagat
      241 cggctgccgc atggcgggcc aggtgctctt ccaccggcac gtgcggggcg aacagcagcc
      301 tgaccggatc gatgccggcg cgccgggctt cctgcatcag atgccgcgtg gcgtcggggc
      361 cgctttcgag cagccagagc acgctgtccg gcaccgcatc aagcagtcgc atccagatcg
      421 cgaagacgtc cggcaggatc ttgtaggcct tgttgaagca gcagaacacg aagcctttgt
      481 cgggcagacc gcatgcaagg cgcgacggcg ctgttccgcg cggcgcgcgc gtggtgtcgc
      541 tcggatagaa gctattcggc atcggccata gccgctcggt gtagtacggc tgtgtcgcct
      601 ccgctgcggc gaagcggtcg accaggatgt agtcgtacca gtcggcgccc agcgtgccgg
      661 gaaagccgat cgaattgatc tgtatcggcg ccggacgctg cgcgaacagg tgctcgcgcg
      721 aatgcgtcgt atagccgttg aggtcaaaca ggatggcgat gcggtcatta cggatgcgtt
      781 gcgcaatcga cgtcagcgtg tcgccgctga cgtccgcata gcgatcgaac gcggcggcga
      841 cccggcgtcc gatcgcgctc tcctggcgcg gcagcaggct gtaggcgcaa gtctcgaacc
      901 gcccgcgatc gatgcgctcc caatgctcga cctgcagatg cgccatcgga tgctcgcgca
      961 ggtccgacga gacgaaaccg atgcgcaggc gctcgccatt gtccatggtc accgtcggcc
     1021 gcggtagcgg cggcgatggt gcgaagccgc gcgcccagcg ctgggctgca tgcagttgcg
     1081 cacgcggcga cgtgggcatc gccagcaccg ggaatgggtt cgccagatgg cgatgcgtcg
     1141 cgttgccatc ctcaagttgc cgattgatcg aggcaaagag ctcgtccaga cccgaccagt
     1201 cgcagcggtg ctggcgcgcg tgcgcgagca tcgacagcgc atacgggttg accggatcga
     1261 gcgtaagcgc gcgcagcagc ggcgcctcgg ccgccgccca gcggtacctc tcggcgcagg
     1321 ccgacgcgag attgatctgc acgtcggtat cgtcgggcgc gattcgcgcg gcttcgcgca
     1381 ggctggcctc ggcaccggcc atgtcgtggc ttcggcgctg cgcgagtgcg cgacgcaccc
     1441 agatgccggc cggcgcgtcg cggcgtatgg caaacgcgcg cgtgtaggat tcggcggcgg
     1501 cggcgaagtc ttcgcgtccg aactggatat tggcgagatt ggccagcgcg tcgggatgat
     1561 tgggggtggc agcgagcacg tcgcggtagc aggcctcggc ttcagcgcta cggccttgcg
     1621 cttcgtaggt gagccccagg ttgttgagca cgccggcatg accgggagca cgcaacagcg
     1681 cgcgccggta atggccgacg gcgaggtcgt gctggccgcg ctcgcgttcc aggttgccca
     1741 gatggaacaa ggcttcggcc tcatccggat cggcatcgag ggcttgccgc caggccgcct
     1801 cggcctgccc ggcatcgcca agggctaagg cgctgcgcgc cttggccaag agcggggcca
     1861 cgccgccggc cgccagcggc ggctgcagcg caccgtggca atccttgtag cgcttgccgc
     1921 tgccgcaggg gcagggcgca ttgcgcggcg gctcagaggc gatggcgctc atcccaggtg
     1981 tgcagcagca gagcttcgaa gtcttgcgta tagcgggcca tgtcgaacag cgggtgggtg
     2041 tggcggttgg cggcgagcct ggtgcgcagg gccgccaggg tttccggctc gcgggccagg
     2101 cgcaaggcga gcgcttcgta ctcggccagg ctcgtcgtca ccagctccgg cagtccgatc
     2161 gcatgcagct gactgccggc aatgcgcgag gccatcgtct cgccgctgca ggtcagcacc
     2221 ggtaccccca tgaacagcgc atcgttcgcc gtcgtgcccg cgttgtacgg cagcgtgtcg
     2281 aggaacagat cagcgtgcgc gtgtcgtgcc cgatgctcgg ccagcccgac ccgcggcgcg
     2341 aaagccagtc gcctcggatc gacgccgcgc gcgatggctt cgcgctgcag gttggcacag
     2401 gcgatcggat atccggggga aagccacagc acactgcccg gaacctcgcg cagcagccgc
     2461 atccagacat cgaacaccgg cggcaggatc ttgtacggcg cgttgaagca ggcgaacacg
     2521 aatccttgc
//
LOCUS       DATANS010000342         2707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_186641, whole genome shotgun sequence.
ACCESSION   DATANS010000342 DATANS010000000
VERSION     DATANS010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186641"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1523)
                     /locus_tag="VHT22_04950"
     CDS             complement(<1..1523)
                     /locus_tag="VHT22_04950"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX3136933.1"
                     /translation="MSVPAAALTASAASSYRRIAVVGVVLLVALAALIWLQPRWNTRL
                     QSTWFDTYQLLKPRGVATSPVTVVEIDDASLAQLGQWPWSRTVLAALIRRIAREQPAA
                     IGVNILMSEPDRLSPDQLVAQTGQVDPTLADRLAALPSSDRELAGAIAAAPVVLGLAG
                     TPATTSRQPLAPPFVVVDRAQRDPSRESASADLPRFAGALTNIELLDRSAAGHGLISA
                     GRSDDAIRRLPLAARIGDGLVPSMPIEMLRVALHAGEVRLFVDGPVVESIGVGDFVVP
                     TEADGELRIYYARRDSHRSVSATDVLDGKVDSLRFEHKLVLIGTTGLAVADYQNTPLG
                     MRMPGSEIQAQVLENLFDQTWLERPRWAPGLELAVFTLSGVMLIAVTPRWKPRNAALL
                     ATACMALPVGAAFFAFFLWRLVFDAAAPALGLLILFSFLLLLSLGEAARQRKRLEQVV
                     QAQREQAAYIDGELAAAKRIQTGFLPRADFLRDDSRVELAAAMTPAREVGGDLYDFFR
                     "
     gene            complement(1520..2479)
                     /locus_tag="VHT22_04955"
     CDS             complement(1520..2479)
                     /locus_tag="VHT22_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3136934.1"
                     /translation="MKCVRFWGTRGSLPVALTASGVRHKLVAALRTASGRTFASDDDI
                     NAYVDTLGMAIGGSYGGHSSCVEIETGGPDYVLCDLGSGARPFAHRAFARHGPASPQT
                     YHIFMSHVHWDHIMGFPFFVPAYIPGNRIHIYGGHAVIEDALRRQQSPPSFPVDFTAL
                     RADIEFVRLEAGQSYDIAGLQVRLMPQRHGGGSYGYRFTAAGRTVVYSTDSEHKLADA
                     AETDAFVDFFRDADLVIFDAMYSLADTMSVKADWGHSSNIVGVELCQMAGARHLCLFH
                     HEPMHDDAAIDRLLAETRRLEEITRPGEALQVSAAYDGMEIRW"
     gene            complement(2476..>2707)
                     /locus_tag="VHT22_04960"
     CDS             complement(2476..>2707)
                     /locus_tag="VHT22_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX3136935.1"
                     /translation="SGVGYISSMGLRLLMIAAKELRSRGARIAVAAMQPIVAEIFDIA
                     RFGHVVEVFPSVRTALQSVSVPALAAYEAATR"
BASE COUNT          516 a          907 c          875 g          409 t
ORIGIN      
        1 cggaagaagt catagaggtc gccgccgact tcgcgcgccg gcgtcatcgc cgcggcgagc
       61 tcgaccctgg agtcgtcgcg gaggaagtcc gcacggggca gaaatccggt ctggatgcgc
      121 ttcgccgccg ccagttcgcc gtcgatgtac gcggcctgct cgcgctgcgc ttggaccacc
      181 tgctcgagcc gcttccgctg ccgcgccgcc tcgcccaggc tgagcaacag caggaaactg
      241 aacaggatca gcaggcccag tgcgggcgcg gccgcgtcga ataccaggcg ccacaaaaaa
      301 aacgcgaaga acgccgcgcc aacaggcagc gccatgcacg ccgtcgcgag cagggcggcg
      361 ttgcgcggct tccagcgtgg cgtgaccgcg atcagcatca ctccggacag cgtgaaaacc
      421 gcgagctcca gacccggcgc ccagcgcggc cgctcgagcc aggtctggtc gaacagattc
      481 tccagcacct gcgcctggat ttcgcttccc ggcatgcgca tgccgagcgg cgtgttctga
      541 tagtcggcca ccgcgagccc ggtcgtgccg atcagcacaa gcttgtgctc gaagcgcaga
      601 gaatcgacct tgccatcgag cacgtctgtt gcggacacgg aacggtggga gtcacgccgc
      661 gcgtaataga tccgaagctc gccatcggct tcggtgggta cgacgaaatc gccgacgcct
      721 atcgactcga cgaccgggcc gtcgacgaac aatcgaactt cgcccgcatg cagcgcgacg
      781 cggagcatct cgatcggcat tgacgggacc aggccgtcgc cgatccgcgc cgccagagga
      841 aggcgccgga tggcgtcgtc ggagcggccg gccgagataa ggccatgccc ggccgcgctg
      901 cgatcgagca gctcgatgtt ggtgagggct cccgcgaagc gcggcaggtc ggccgatgcc
      961 gattcccgcg atggatcgcg ctgcgcacgg tcgacgacga cgaatggcgg cgcgagcggt
     1021 tggcgggacg tcgtcgcggg tgtaccggcc agcccgagga ccaccggcgc cgcggcaatc
     1081 gcgccggcaa gctcgcggtc gctcgagggc agcgcggcca ggcggtccgc cagcgtcggg
     1141 tcgacttgcc ccgtctgcgc gaccaattga tccggcgaca ggcggtccgg ctcggacatc
     1201 aatatgttta ctccgatggc cgccggttgc tcgcgcgcaa tgcggcgaat cagcgcggcc
     1261 agcacagtgc gcgaccacgg ccactgtccg agctgcgcaa ggctcgcgtc gtcgatttcg
     1321 accaccgtga cgggtgacgt cgcaacgccg cgcggtttca acagctggta cgtatcgaac
     1381 caggtggatt gcagccttgt gttccagcgt ggttgcagcc atatcagcgc ggctagcgcg
     1441 acgagaagga ccacaccgac cactgcgata cggcggtacg acgaagctgc ggacgcagtc
     1501 agcgccgcag ctggaacgct caccaacgta tttccattcc gtcgtaggct gcgctgacct
     1561 gcagcgcctc tccggggcgc gtgatctcct cgagtcgccg cgtttccgcg agcaggcggt
     1621 cgatcgccgc gtcatcatgc atcggttcgt gatggaacag gcaaagatgc cgtgccccag
     1681 ccatctggca gagctcgacg ccgacgatgt tgctcgaatg gccccaatcc gccttgaccg
     1741 acatcgtatc cgccagcgaa tacatcgcgt caaagatcac caggtcggcg tcgcgaaaaa
     1801 agtcgacgaa agcgtcggtt tccgcagcat cggccagctt gtgttcggag tcggtcgaat
     1861 agacgaccgt ccttccggcc gccgtgaaac gatagccgta cgaccctccg ccatgtctct
     1921 gcggcatcag tctgacctgc aacccggcaa tgtcgtacga ctgtcctgcc tccagccgca
     1981 cgaactcgat gtccgcacgc agcgcagtaa aatcgaccgg gaaagagggg gggctctgct
     2041 gccgccgcaa cgcgtcttcg atcacggcat gaccgccata gatatggatg cggttgcccg
     2101 gaatgtatgc cggcacaaag aacgggaacc ccatgatgtg gtcccaatgc acgtgcgaca
     2161 tgaagatgtg ataagtctgt ggcgacgccg gcccgtgacg cgcgaatgcc cggtgagcga
     2221 acggacgagc gccggagccc aagtcgcaga gcacatagtc cggtccaccc gtctcgatct
     2281 cgacacacga cgaatgaccg ccgtagctgc caccgatggc catcccgagc gtgtcgacgt
     2341 acgcgttgat gtcgtcgtcg ctggcgaacg tacgacccga cgcggtacgc agcgccgcga
     2401 cgagcttgtg ccggacaccc gatgccgtaa gcgcgaccgg cagggatccg cgcgtacccc
     2461 agaagcgaac acacttcatc gggtagcggc ctcgtacgcc gccagcgccg gcaccgacac
     2521 tgactgaagt gctgtgcgca ccgagggaaa gacttcgacc acgtgcccga agcgcgcgat
     2581 gtcgaagatt tccgcaacga tcggctgcat ggcggcgacg gcgatccggg cgccacgaga
     2641 gcgcagctcc ttcgccgcga tcatcaacag acgaagtccc atgctgctga tgtatccgac
     2701 ccccgag
//
LOCUS       DATANS010000343         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_59568, whole genome shotgun sequence.
ACCESSION   DATANS010000343 DATANS010000000
VERSION     DATANS010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59568"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VHT22_04965"
     CDS             <1..335
                     /locus_tag="VHT22_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX3136936.1"
                     /translation="IIARFSADAAGKLARAGIVNARLEVGDCARGSDNRQYDAIVLTG
                     STPVLPDSWLQQLKPGGRLFAVVGDPPVMTARLVHSTAPGAFATEDLFETVIAPLRNA
                     AQPARFVF"
     gene            422..>1502
                     /locus_tag="VHT22_04970"
     CDS             422..>1502
                     /locus_tag="VHT22_04970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEX3136937.1"
                     /translation="MVKRLLAIALGAACSGALSPAAADDLLQVYRDALTSDPVLASAR
                     ANWAAIQENVPQARAGLLPNVGLAANATYEDFYERIHVDPSPTIREGFPQTGYTVSAS
                     QPLYRPQNSIALSQARQQVGQSDFVLSSAQQDLIVRVTQAYFDVLLARFNIELTESQK
                     AAVSENLAQAKRNFEVGTATITDTNDAQAKFDQIVAQEIAARTDLDNKLAALRAILGH
                     LPRDLKRFGGRFDPRLPSPNALEPWIEQALSQNLQVRIAQANFDIASLEVDRQRAAHY
                     PTVDLVASFNQSYAGGSASTQLSSSAAFDSRLAQIGVQLNVPLYQGGAIDSRVRQAIA
                     NQDRARQDLETARRGAQLLAQTSFSG"
BASE COUNT          262 a          501 c          491 g          248 t
ORIGIN      
        1 agatcatcgc gcggttttca gccgatgcgg ccggcaagct ggcccgcgcc ggcatcgtca
       61 atgcgcgcct ggaggtcggc gactgcgcgc gcggatcgga caatcggcaa tacgacgcga
      121 tcgtgctcac cggttcgacg ccggtcctgc ccgattcgtg gctgcagcag ctgaagccgg
      181 gtggccggct attcgccgtc gtcggcgatc ctccggtgat gaccgcacgc ctggtccact
      241 cgacggcgcc gggtgcattt gcgaccgagg accttttcga gacggtgatc gcgcctttgc
      301 gcaatgccgc gcagccggcg cgcttcgtat tttgaatccg ttcgggcagc agcaggggtt
      361 cccgcgcgag cggcgagcca cggcccaacc tgatcctcga ccgactgact ggaccgctac
      421 gatggtgaaa agactgcttg cgatcgcgct cggcgctgcc tgcagcggcg cgctttctcc
      481 cgccgctgcg gacgacctgc tgcaggtcta tcgcgacgcg ctgaccagcg atccggtgct
      541 tgcgtcggcg cgtgccaact gggcggcaat acaggaaaac gtgccgcagg cgcgcgcggg
      601 gctgctgccg aacgtaggtc tcgccgcgaa cgcgacttac gaggacttct acgagagaat
      661 ccacgtcgat ccttcgccaa cgatccgcga agggtttccg cagaccggct acacggtgtc
      721 tgccagccag ccgctgtatc gtccgcagaa ttcgatcgcg ctctcgcagg cgaggcagca
      781 ggtcggacag agcgatttcg tgctgtcgtc ggcgcaacag gacctgatcg tccgcgtcac
      841 gcaggcctat ttcgacgtgt tgctggcgcg gttcaacatc gagctgaccg agagccagaa
      901 ggccgcggtc agcgaaaacc tggcgcaggc caagcgaaat ttcgaggtcg gcaccgcgac
      961 gatcaccgac accaacgacg cgcaggcgaa gttcgaccag atcgtcgcgc aggaaatcgc
     1021 cgcgcgcacg gatctcgaca acaagctcgc agcgctgcgt gcgatccttg ggcacctgcc
     1081 ccgggacctc aagcgcttcg gcggcagatt cgaccctcgt cttccctctc cgaacgcgct
     1141 cgagccgtgg atcgagcagg cgttgagcca gaatctccag gtgcgcatcg cgcaggccaa
     1201 cttcgacatc gcgagcctcg aagtggatcg gcaacgcgcc gcgcactacc cgacggtcga
     1261 cctggtagcg agttttaacc agagttacgc cggcgggtcg gcgtcgacgc agctgagttc
     1321 gtcggccgcg ttcgattcgc ggttggcaca gatcggtgtg cagctcaacg tgccgctcta
     1381 ccagggaggc gcaatcgatt cgcgcgtgcg gcaggcgatc gccaaccagg accgcgcccg
     1441 ccaggatctc gaaacggcgc gacgcggcgc gcagctgctc gcgcaaacat cgttctccgg
     1501 gg
//
LOCUS       DATANS010000344         3644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_226793, whole genome shotgun sequence.
ACCESSION   DATANS010000344 DATANS010000000
VERSION     DATANS010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226793"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VHT22_04975"
     CDS             <1..549
                     /locus_tag="VHT22_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin deacetylase"
                     /protein_id="HEX3136938.1"
                     /translation="LHEGVDPGDAEPELLERTHKLLTDITGIAPRGWRSPSGHKTART
                     LHKLRELGYVYDSSDKDFDLPYLLRYGGQEHDNYVCLPNNTSSLDDFPFYRVSYTPPS
                     EVLTHWMQEFDAIYAEGGYFNLTVHPRVGYGSGSPARVAIVEKLIEYIKRHDGVRFAG
                     MLELAQWCLKRPADWRRDWSGQ"
     gene            546..1340
                     /locus_tag="VHT22_04980"
     CDS             546..1340
                     /locus_tag="VHT22_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX3136939.1"
                     /translation="MNAQIRPVAVTVNLQGKTVELQQLGEDALFGRASYGKYAYAVGC
                     ERLFGLLDRHGIKATVFVPGAEAEANPDYVATLAHRGHEIAAHGWAMEAMDAPGIDER
                     ALIERTHSTLTRIIGASPHGFRAPHGKLTERTLGHLAALGYRYDSSFQDDDRPYRLDA
                     DGGSGMIEVPQSEILIDATLYAQRQTHDRVMKTWHEEIEAMHRERCLITLTLHPRSDY
                     GSARASRIAALDRLLTWLRDLPGVAFMRCDAIATAADEFHSETRRG"
     gene            complement(1374..2318)
                     /locus_tag="VHT22_04985"
     CDS             complement(1374..2318)
                     /locus_tag="VHT22_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX3136940.1"
                     /translation="MPVPILLVTGFLGAGKTTVVNHLLAHAEGRRIAAVVNDFGAINI
                     DAELIAGASDGVVSLSNGCICCSLEGDLLRTLATLLRRDPRPEFIVIETSGIADPSDI
                     VRNLMDPVIWQEAPLETVLCVVDATTNAAALNDALLRSQLRAADVVALSKVDLVDAAG
                     RAQLCDVVRALRPGAVVVDALHGEVPAALLFPADVDRALALREVRPRRPTADRFETLS
                     WTSERPVSLPRLQQAISRLAPKLARAKGLFETVEQPGRLMVFQLAGGRATLAPGGVLA
                     AGALAARVPRTRIVFIAEIGVLSSAEIDRIMDACIEAG"
     gene            complement(2321..>3644)
                     /locus_tag="VHT22_04990"
     CDS             complement(2321..>3644)
                     /locus_tag="VHT22_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3136941.1"
                     /translation="IDLHAHGQSIPADRMQAFDGVTTTLDLEAGVLPVASWYRRQADK
                     GRVLNYGAAANWAFARIGAMTGSNAESSLEAFGNAMRDRRWIENVASDTEVAGILDRL
                     ANGLNEGGIGIGILNAYAPGAGVQELTAVCQLAATHDVATFTHVAYMSRIDPESAAEA
                     YIRLIGYAGATGAHMHICHFNSSSKTDIERCTALIAKAQAQGLPITVEAYPYGTGSTV
                     LAAAFFSDPEFEERNGLGYDSVQRVTDGHRFRDRKELLAAQAEEPSTLVLWHVLDIEN
                     NPHHRNLLDMAVLYPGGAIASDAMPWTMPDGRPYTGDAWPLPDDATSHPRSAGCFTRF
                     IREWVRERKKVSLLEGIRKCALIPAEILSHSTPAMRAKGRLQPGADADIVVFDFETLS
                     DKATFSAMNRPSEGVRHLVVSGQPLITDGVLDVAARPGRPVRRPVVES"
BASE COUNT          644 a         1262 c         1159 g          579 t
ORIGIN      
        1 ctgcacgaag gtgtcgaccc cggtgacgcc gagccggagt tgctggaacg cacgcacaaa
       61 ctgctgaccg acatcaccgg catcgcacca cggggctggc gctcgccctc cggacacaag
      121 accgcgcgca cgctgcacaa gctgcgcgag ctcggttacg tctacgattc cagcgacaag
      181 gatttcgacc tgccctatct gctgcggtac ggcggtcagg agcacgacaa ctacgtctgc
      241 cttcccaaca acacctcgag cctcgacgac tttccgttct atcgcgtcag ctacaccccg
      301 ccgtcggaag tgctgacgca ttggatgcag gagttcgacg ccatctacgc cgagggcggc
      361 tatttcaacc tgaccgtcca tccccgcgtc ggttacggtt ctgggtcgcc ggcgcgggtg
      421 gcgatcgtcg agaaactgat cgaatacatc aagcgtcatg atggcgtgcg cttcgccggc
      481 atgctggagc tggcgcaatg gtgcctgaaa cgcccggccg actggcgacg ggactggagc
      541 ggccaatgaa tgcgcagatc cgtccggtcg cggtgaccgt caatttgcag ggcaagaccg
      601 tcgaactgca acagctcggc gaggacgcgc tgttcggccg cgcctcctac ggcaaatacg
      661 cctacgcggt tggctgcgag cggctgtttg gactgctcga ccggcacggc atcaaggcga
      721 cagtgttcgt gccgggggcg gaagccgaag ccaatcccga ctatgtcgcc accctcgccc
      781 accgcggcca tgagatcgcc gcgcacggct gggcgatgga agcgatggac gcgcccggta
      841 tagacgaacg cgcactgatc gagcgaacgc atagcacgct gacgcgcatc atcggtgcga
      901 gcccgcatgg attccgcgcg ccgcacggca aactcaccga gcggacgctg gggcacctcg
      961 cggcgctcgg ttaccgctac gattcaagct tccaggatga cgaccggccc taccggctcg
     1021 atgctgatgg cggcagtggc atgatcgagg tgccgcagtc ggaaatcctg atcgacgcca
     1081 cgctgtacgc gcagcggcag acccatgacc gggtcatgaa aacctggcat gaggagattg
     1141 aagccatgca ccgcgagcgc tgcctgatca cgctcacgct gcatccgcgc agcgactacg
     1201 gcagcgcgcg tgcgagccgt atcgcggcgc tcgatcggct gctgacgtgg ctccgcgacc
     1261 tcccgggcgt tgcattcatg cgctgcgacg cgatcgcgac ggcggccgat gagtttcatt
     1321 cggaaacccg ccggggatga agaacggccg agttcggatc cgctccgctc acttcacccg
     1381 gcttcgatgc acgcgtccat aatccgatcg atctcggcgc tcgagagcac cccgatctcg
     1441 gcaatgaaga cgatccgcgt ccgcggtacc cgggccgcca gtgccccggc cgcgagcacc
     1501 ccgcccggtg ccaatgttgc gcgcccgccg gcgagctgaa acaccatcaa ccgcccgggc
     1561 tgctccaccg tctcgaacaa cccctttgcc cgcgcaagct tcggcgccag ccgactgatt
     1621 gcctgctgca accggggcaa cgacaccgga cgttccgatg tccagctcag cgtctcgaac
     1681 cggtcagccg tcggccgtcg cggccttacc tcgcgcagcg caagcgcacg gtccacgtcc
     1741 gcggggaaca gcagcgctgc cgggacttcg ccatgcaggg catcgaccac caccgcaccc
     1801 ggacgcagcg cccggacgac atcgcagagc tgtgcgcgac cggccgcgtc gaccaggtcc
     1861 accttgctca gcgccaccac gtcggccgca cgcagctgag agcgcagcaa cgcgtcgttc
     1921 aatgctgccg catttgtcgt cgcgtccacc acgcacagca ccgtttccag cggcgcctcc
     1981 tgccagatca cggggtccat caggttgcgc acgatatccg agggatcggc gatcccgctg
     2041 gtctcgatca cgatgaactc cggccgcgga tcgcgccgca gcagggtggc gagcgtgcgc
     2101 aagagatcgc cttccagcga gcagcagatg cagccattgc tcaggctgac cacgccatcg
     2161 ctggcgcccg cgatcagctc cgcgtcgata ttgatggcgc cgaaatcgtt gaccacggcg
     2221 gcgatgcgcc ggccttccgc atgtgccagc agatgattga cgacggtcgt cttgcccgcg
     2281 ccgagaaacc cggtcaccag caggatcggg accggcacca tcagctctcg acaacgggac
     2341 ggcgcaccgg ccggccgggc ctcgccgcca cgtccagcac gccatccgtt atcagcggct
     2401 gaccgctgac caccagatgc cggacgccct ccgacgggcg gttcatcgcc gagaacgtcg
     2461 ccttatcgct cagcgtctcg aaatcgaaca cgacaatgtc ggcatccgcg ccgggctgca
     2521 gcctgccctt ggcgcgcatc gcgggcgtgc tgtgggaaag gatctctgcc gggatcagcg
     2581 cgcatttgcg gatgccttcc agcagcgaca ccttcttgcg ctcacgcacc cactcgcgga
     2641 tgaaacgggt gaagcagccc gccgagcgcg ggtgcgaggt ggcgtcatcc ggcagcggcc
     2701 aagcgtcgcc ggtgtaaggc ctgccgtccg gcatcgtcca cggcatcgcg tcggacgcga
     2761 tcgcgccgcc gggatacagc accgccatat ccagcaggtt acgatggtgc ggattgttct
     2821 cgatatccag gacatgccag agcaccagcg tggatggctc ctcggcctgc gccgccagca
     2881 gttccttgcg gtcgcgaaaa cggtgtccgt cggtcacgcg ctgcaccgaa tcatagccga
     2941 ggccattgcg ctcctcgaac tcgggatcgc tgaagaaggc cgccgccagc accgtcgaac
     3001 cggtgccgta gggataggcc tccaccgtga tcggcaggcc ctgcgcctgc gccttggcga
     3061 tcagcgcggt acaacgctcg atgtccgtct tgctggaaga gttgaagtgg cagatgtgca
     3121 tgtgcgcgcc ggtggcgccg gcatagccga tcaggcggat ataggcttcc gcagcgcttt
     3181 ccggatcgat gcgcgacatg taggcgacgt gggtgaaggt cgcgacatca tgcgtcgccg
     3241 ccagctggca caccgcggtc aattcctgga cgccggcgcc cggcgcatag gcgttgagaa
     3301 tgccgatgcc gatgccgccc tcgttcagtc cgttggcaag gcggtctaga atgccggcca
     3361 cctcggtgtc gctggccacg ttctcgatcc agcggcgatc gcgcatggcg ttgccgaacg
     3421 cctccagcga gctttcggca ttcgagccgg tcatcgcacc gatgcgcgca aacgcccagt
     3481 tggcggcggc gccatagttc agcacgcggc ccttgtcggc ctggcgccga taccaggatg
     3541 cgaccggcaa gacgcccgcc tcgaggtcga gcgtcgtcgt cacgccgtcg aacgcctgca
     3601 tgcggtcggc cgggatcgat tggccgtgcg cgtgcagatc gatg
//
LOCUS       DATANS010000345         2243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_522087, whole genome shotgun sequence.
ACCESSION   DATANS010000345 DATANS010000000
VERSION     DATANS010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522087"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1203)
                     /locus_tag="VHT22_04995"
     CDS             complement(175..1203)
                     /locus_tag="VHT22_04995"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX3136942.1"
                     /translation="MTAATTQQGALLDADTPDTIRMTPEAAGELAVAALKRVGFTDED
                     AGIIADQLVDNALCGYRFASLPRILAIAGNNKTQNERRPIKVVHETPLSALIDGGNNL
                     GYVSVYKATEVAINKARDRGFAMVGVYDSYYSGRSAYFMEMIAKAGFVGIHAASTRPH
                     VLPVGGIKPIFGTNPLCIGFPSSKGPVIYDIGTASIMVGEVQLFAHIGQELPEGIAFD
                     EHGNPTRDPNAALRGGWVPFGGHKGYGLSFAVQALGLLAGAGLAQSKVKDYGFLLLVI
                     DPKLTLPGGEFPDHMAELVDKIKATPRRPGVDEIRVPSERSFRERERRRVEGLVFDRK
                     VVDSLKAL"
     gene            1321..1797
                     /locus_tag="VHT22_05000"
     CDS             1321..1797
                     /locus_tag="VHT22_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX3136943.1"
                     /translation="MQIKFTLNGEPVETHLKSGDRLLVDVLREDLQLTGTKHGCGIGA
                     CGTCTVIVDGLPVASCLQLAALVDGADVRTVEGLAGAASLHVVQQAFINAAAMQCGIC
                     TPGHVMAACALLAKTPTPTDAEIRTSVESVYCRCTGYVRIEAAYREAVALAKKTRA"
     gene            1794..>2243
                     /locus_tag="VHT22_05005"
     CDS             1794..>2243
                     /locus_tag="VHT22_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX3136944.1"
                     /translation="MNQRETTLRPAGSLLVVGKSVPRTDAIPKVTGAAQYVADLHMPG
                     MLHAAVLRSPHPHARIVGIDITAAAALPGVKAVITGADTARKKWGCFRPDLYPLALDK
                     VRYVGDEVAAVAARDPQTARAAIDLIKVEYEVLPAVLSLDAALAPGAP"
BASE COUNT          420 a          735 c          698 g          390 t
ORIGIN      
        1 accagcgttc gagcttgcgc cggtcgaaat tatgctcgac ggagaactgt tcgatgaaaa
       61 ccgcgacgtc cgaatcaggc gcgatggaag cggcgtgcag gtggccggat tgcgcgagca
      121 aagatgcgca caatgccagt cctgccagcg cgagacgcgc gattgacgcg acggtcacag
      181 cgctttcagc gaatcgacga ctttgcggtc gaagaccaat ccttcgaccc gccggcgctc
      241 gcgttctcga aacgagcgct cggacggaac gcggatttcg tccacgccgg gacgccgcgg
      301 cgtcgccttg atcttgtcga cgagttccgc catgtgatcg ggaaattcgc cgccgggcag
      361 cgtcagcttg ggatcgatga cgaggagcag aaaaccgtag tccttgactt tgctctgcgc
      421 gaggcccgcg ccggcgagta gtccaagtgc ctgtactgca aacgacaagc cgtaaccctt
      481 gtggccgcca aacggtaccc agccgccgcg caatgccgca ttggggtcgc gcgtcgggtt
      541 gccgtgctcg tcgaacgcaa tgccttcggg cagttcctgg ccgatgtgcg cgaacaattg
      601 cacttctccg accatgatgg atgcagttcc gatgtcataa atgaccggcc ccttcgacga
      661 cgggaaaccg atgcacagcg gattggtgcc gaatatcggc ttgatgcctc cgaccggcag
      721 aacatgcggc cgcgtgcttg ccgcatgaat gccgacgaac cccgccttcg cgatcatttc
      781 catgaaatag gcgctgcggc cgctgtaata actgtcgtac acgccgacca tcgcaaagcc
      841 gcggtcacgc gctttattga ttgcgacctc ggtcgcctta tacaccgaca catagccgag
      901 attgttgccg ccgtcgatca gcgccgacag cggcgtctca tgcacgactt tgatcggccg
      961 gcgctcgttc tgcgtcttgt tgttgccggc aatcgccaga atgcgcggca gactcgcaaa
     1021 ccggtaaccg cacagcgcgt tgtcaaccag ctggtccgcg atgattccgg cgtcttcgtc
     1081 cgtaaagccg acgcgcttca gtgcggctac ggcaagctcg ccggcagcct caggcgtcat
     1141 tcggatggtg tcgggcgtat cggcgtcgag caaggcgccc tgttgcgtgg tagccgcggt
     1201 catgcggaac ttcctctagt gttagctaag agagccgatt ataagcggat gtctgccggc
     1261 agaaagcgtt gtgcgtgcga tgccttataa tccggccacc gatcgcgaga aaatttcgac
     1321 gtgcaaataa aatttacgct caacggcgaa ccagtcgaga cgcatctcaa gagcggcgac
     1381 cgcctgctcg tcgacgtctt gcgcgaagac ctgcaattga ccggcaccaa gcacggctgc
     1441 ggcatcggcg cctgcggcac ctgcaccgtg atcgtcgacg gcctgcccgt tgcttcgtgc
     1501 ctgcagctcg ccgcgctcgt cgacggcgcg gacgtgcgca cagtcgaagg cttggccggc
     1561 gccgcgtcgc tgcacgtcgt ccagcaggcg tttatcaacg cggcggcaat gcagtgcggc
     1621 atctgcacgc ctggccacgt gatggcggcg tgcgcgctgc tcgcaaaaac ccctacaccc
     1681 acggacgccg agatccgcac cagcgtcgaa agcgtctatt gccgctgcac cggctacgtg
     1741 cgcattgaag ccgcctaccg cgaagcggtc gcgctcgcga aaaaaacgcg tgcatgaacc
     1801 agcgcgaaac taccctgcgt cccgccggca gcctgctggt cgtcggcaag agcgtgccgc
     1861 gcaccgacgc gattcccaag gtgacgggcg cggcgcaata cgtcgcagac ctgcacatgc
     1921 cgggcatgct ccacgcggca gtgctgcgca gcccgcatcc tcacgcccgc atagtcggca
     1981 tcgacattac ggccgcagcg gctctgccgg gcgtcaaggc ggtcattacc ggcgccgaca
     2041 cggcgcgcaa gaaatggggc tgcttcaggc ccgacctcta cccgctcgcg ctcgacaagg
     2101 tgcgttacgt cggcgacgaa gtcgctgccg tagcggcgcg cgacccgcaa accgcgcgcg
     2161 cggcgatcga cctgatcaag gtcgaatacg aggtgctgcc cgcggtgctg tcgcttgacg
     2221 cggcgctcgc gcccggcgcg ccg
//
LOCUS       DATANS010000346         1903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_17105, whole genome shotgun sequence.
ACCESSION   DATANS010000346 DATANS010000000
VERSION     DATANS010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17105"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /gene="pyrD"
                     /locus_tag="VHT22_05010"
     CDS             complement(<1..742)
                     /gene="pyrD"
                     /locus_tag="VHT22_05010"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HEX3136945.1"
                     /translation="MLYPLFRPLLFTLDPETAHDVAFVALDAAAASGAAQLTAPRVAA
                     SPVLAMGLEFPNRVGLAAGLDKNAAHIDGLATLGFGFIECGTVTPRPQQGNPKPRLFR
                     LPEAQALVNRMGFNNDGVDRFLANVARSRFATKRGGILGLNIGRNFDTPNERAADDYL
                     TCLSAVYRDASYVTVNISSPNTSSLRELQDGAALQPLLAALKAEQAKLSQKHGKYTPL
                     VVKIAPDLSVPDIERIARTLVEQRIDGVI"
     gene            883..>1903
                     /gene="mltB"
                     /locus_tag="VHT22_05015"
     CDS             883..>1903
                     /gene="mltB"
                     /locus_tag="VHT22_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEX3136946.1"
                     /translation="MLDATARGALRCLLFSLAIRVATVLDAAAIEGVAPLRYDKRPET
                     RAFVAELVAEHGFARRDLEHLFAQARYQPRIVAAMSRPVLSPPKWYEYAPQFLSRERI
                     DGGLEFWRTNAPTLARAQSEFGVPAEVIVAIIGVETFYGRNTGSYRVFDALTTLAFDY
                     PRRSDFFRSELRQFLLLARDQGISPLVPRGSYAGASGLPQFMPGSVRAYALDYDGDGR
                     IDLSAEPADAIGSVANYLARHDWQPGAPVLAQARIDPAASEAVLRKLDGGVSERRPLD
                     AWIRDGVGADPLPADPGSDPVGLLMLEEADGPSYWLAFGNWYVLTRYNRSRLYATAVW
                     QLAQAL"
BASE COUNT          302 a          628 c          651 g          322 t
ORIGIN      
        1 cgatgacgcc gtcgattcgt tgttcgacca gcgttcgcgc gatccgctcg atgtccggca
       61 ccgacagatc gggagcgatc ttgaccacca gaggcgtgta tttgccgtgc ttttgcgaaa
      121 gcttcgcctg ttcggccttc agcgccgcca gcagcggctg caacgcggcg ccatcctgca
      181 gctcgcgcag gctcgacgta ttcggcgagg aaatgttgac cgtcacataa ctcgcgtcgc
      241 ggtagacggc ggaaagacac gtcaggtagt cgtcagcggc gcgctcgttg ggcgtgtcga
      301 aattgcggcc gatgttcaat cccaggatac cgccgcgctt ggtagcgaag cgcgagcgcg
      361 cgacgttagc cagaaagcga tcgacgccgt cgttgttgaa ccccatccga ttgaccagcg
      421 cttgggcctc gggcagccgg aacaggcgcg gcttcgggtt gccctgctgc ggccgcggcg
      481 tcaccgtgcc gcattcgatg aatccgaatc cgagcgttgc caggccgtcg atatgcgccg
      541 cgttcttgtc cagccccgcc gccaatccga cgcggttggg aaattcgagg cccattgcca
      601 ggaccggcga cgctgccacg cgcggcgcgg tcagctgcgc ggcgccgctg gccgcggccg
      661 catcgagcgc gacgaacgcg acgtcgtgcg cggtctcggg atcgagcgtg aacagcagcg
      721 gtcgaaacag cggatagagc atcggcgaag gtctttcgaa aacgcccgat gaggaatgcc
      781 aagctgacgg cgcgcggatg ataacattcg ccgcgcatga tcttgttccc tgagcgagcc
      841 gccacggtcg cgcttgcgcc gcgcgcgcgg cgggacgtcg caatgctcga cgcaacggcg
      901 cgcggcgctc ttcggtgctt gctgttctcg ctggcgatcc gggtcgcgac ggtgctcgat
      961 gccgcggcga tcgaaggcgt cgcgccgctg cgctacgata agcgccccga gacgcgggcc
     1021 tttgtcgccg agctcgtcgc cgagcatgga ttcgcgcgcc gggacctcga acacctgttc
     1081 gcgcaggcgc gctatcagcc gcgaatcgtc gcggcgatgt cgcggccggt gctctcgccg
     1141 ccgaaatggt acgaatacgc gccgcaattc ttgagccgcg agcgcatcga cggcggtctc
     1201 gagttctggc ggaccaatgc gccaacactc gcgcgcgcgc aaagcgaatt cggcgttccg
     1261 gccgaggtga tcgtcgcgat catcggcgtg gaaacgttct atggccgcaa caccggcagc
     1321 taccgggtgt tcgacgcgct gaccacactc gccttcgact atccgcgacg cagcgatttt
     1381 ttccggagcg agctcaggca gtttctgctg ctcgcgcgcg accagggcat ttcgccgttg
     1441 gtgcccaggg gttcgtacgc cggcgcatcg ggcttgccgc aattcatgcc gggcagcgtc
     1501 cgggcctacg ccctcgacta cgacggcgat ggccggatcg acctgtcggc cgagccggcc
     1561 gatgcgatcg gcagcgttgc aaactatctg gcgcggcatg attggcagcc gggcgcaccg
     1621 gtgctcgccc aggcgcggat cgatccggcg gcgagcgaag ccgtgttgcg caagctcgac
     1681 ggcggcgtca gcgagcggcg gccgctagac gcctggatac gcgacggcgt cggcgccgac
     1741 cccttgccgg ccgaccccgg ttccgaccca gtcggcttgc tgatgctgga ggaagccgac
     1801 gggcccagct attggctggc attcggcaac tggtatgtgc tgacgcgcta caaccgcagc
     1861 cggctctatg cgacggccgt gtggcaactg gcgcaggcgc tgg
//
LOCUS       DATANS010000347         2614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_313022, whole genome shotgun sequence.
ACCESSION   DATANS010000347 DATANS010000000
VERSION     DATANS010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313022"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VHT22_05020"
     CDS             <1..462
                     /locus_tag="VHT22_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHB accumulation regulatory domain-containing
                     protein"
                     /protein_id="HEX3136947.1"
                     /translation="IDFQVVDAKTGDDLTRAILLQIILDEEGGGVPMFSSEMLSQMIR
                     FYGNALQGIMGNYMEQNVKAFMAIQGKLQDQAKQIYGDKMMLTPDLWKQFLQMQAPAM
                     QGMLGNYLEQSAKLFMDMQQRMQDQTRGLFTAFPFPNFGATQPSGDDEKKR"
     gene            527..1918
                     /gene="rimO"
                     /locus_tag="VHT22_05025"
     CDS             527..1918
                     /gene="rimO"
                     /locus_tag="VHT22_05025"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX3136948.1"
                     /translation="MTPKSAPSRPRSRPEPGPRVGFVSLGCPKALVDSEQILTRLSAE
                     GYAIAPTYDGADVVVVNTCGFIDAAVEESLSAIGEALAENGRVIVTGCLGAKEGGAFV
                     RAAHPKVLAVTGPHAASEVMAAVREHLPRPHDPFVDLLPPPNSKIGIKLTPKHYAYLK
                     ISEGCNHRCTFCIIPSLRGDLVSRPIGEVMIEAENLLKAGVREILVVSQDTSAYGVDV
                     RYRTGFWRGRPLKTRMTELSAALATLAAEYGAWVRLHYVYPYPHVDETIALMASDGGP
                     GLLPYLDVPFQHASPRILKLMKRPANVENNLQRIRAWRAACPELTIRSTFIVGFPGET
                     EAEFEALLEFLSEAELDRVGCFAYSPVDGAAANALPDPVPDALREERRARFMSTQEAI
                     STRRLKRRIGATLRVLVDSHDGARALARSAADAPEIDGTVAVADGAALAVGEFARVRI
                     TGASAHDLSGVRA"
     gene            complement(1932..2561)
                     /locus_tag="VHT22_05030"
     CDS             complement(1932..2561)
                     /locus_tag="VHT22_05030"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEX3136949.1"
                     /translation="MNPAVSLSRRRALASALALTIAACGALSRPSPVKSTYLLEPPAP
                     SNKSATPGAATLKVEAVAVAAPFRGRTLVYRESDLKYEADFYEEFLVSPATMIGEATA
                     SWLAATGIYRAVLPPSTSLEGDFALEAFVTDLYADLRDPAHPASVLKIKFFLSSSAAA
                     SGTFVWNGELGSRIDVASRSADALVRGFNTALSNVLDQLAAVLRNLPQK"
BASE COUNT          451 a          856 c          894 g          413 t
ORIGIN      
        1 atcgacttcc aggtcgtcga cgccaagacg ggcgacgacc tgacgcgggc gatcctcctg
       61 cagatcatcc tcgacgagga aggcggcggc gtgccgatgt tcagctccga gatgctctcg
      121 cagatgatcc gtttttacgg caatgcattg cagggcatca tgggcaatta catggagcag
      181 aacgtcaagg cgttcatggc catccagggc aagctgcagg accaggccaa gcagatctat
      241 ggcgacaaga tgatgctgac cccggacctg tggaagcagt ttctgcagat gcaggcgccg
      301 gcgatgcaag gcatgctcgg caactatctc gagcaaagtg ccaagctgtt catggacatg
      361 cagcagcgga tgcaggacca gacgcgcggg ctgtttaccg ccttcccctt ccccaatttc
      421 ggcgcgacgc aaccatcggg cgacgacgag aaaaagcgct gatgccgcga agcgccgcaa
      481 catccgcatt gcagcgcgcc atatttgcgc gccgcacgat aggccgatga cgccaaagtc
      541 cgccccttcg cgccctcgct cgcgccccga accggggccg cgggtgggct ttgtctcgct
      601 cggctgtccg aaggcgctgg tcgactccga gcagatcctg acgcggcttt ccgccgaggg
      661 ctatgcgatc gccccgacct acgacggcgc cgatgtggtg gtcgtcaaca cctgcgggtt
      721 catcgacgcc gcggtcgagg agtcgctgtc ggcgatcggc gaggcgctgg ccgagaacgg
      781 cagggtcatc gtcaccgggt gcctcggcgc caaggaaggc ggcgcattcg tgcgcgccgc
      841 gcatccgaag gtgcttgcgg tcaccgggcc gcacgccgca tccgaggtga tggcggcggt
      901 gcgcgagcat ctgccgaggc cgcacgatcc gttcgtcgat ctgctgccac ccccgaacag
      961 caagatcggc atcaagctga cgccgaagca ctatgcctat ttgaagatca gcgagggctg
     1021 caaccaccgc tgcacgttct gcatcattcc ctcgctgcgc ggcgatctgg tcagccggcc
     1081 gatcggcgag gtgatgatcg aggcggaaaa cctgctgaag gccggcgtgc gcgagatcct
     1141 cgtcgtgtcg caggacacca gcgcgtacgg tgtcgacgtc cgctatcgca ccggattctg
     1201 gcgcggcagg ccgctgaaga cgcggatgac cgagctctcg gcggcgctcg cgacgctagc
     1261 cgccgaatac ggcgcctggg tgcgcctgca ctacgtctat ccgtacccgc atgtcgacga
     1321 gaccatcgcg ctgatggcca gcgacggcgg ccccggcctg ctgccgtatc tcgacgtgcc
     1381 gttccagcat gcgagcccgc gcatcctcaa gctgatgaag cgcccggcga acgtcgagaa
     1441 caatctgcag cggatccggg cctggcgcgc cgcatgtccg gagctgacga tacgctcgac
     1501 gttcatcgtc ggatttcccg gcgagaccga ggccgaattc gaagcactgc tcgaattcct
     1561 gagcgaggcc gaactcgacc gggtcggctg cttcgcgtat tccccggtcg atggcgcggc
     1621 tgccaacgcg ctgcccgatc cggttccgga cgcgctgcgc gaagagcggc gcgctcggtt
     1681 catgtcaacg caggaagcca tcagcactcg ccggttgaag cgacggatcg gcgcgacgct
     1741 gcgggtgctg gtcgactcgc acgatggcgc acgggcgttg gcgcgcagcg ccgccgatgc
     1801 gccggagatc gacggcactg tagccgtggc cgatggcgcg gcgctcgccg tcggcgagtt
     1861 cgcccgcgtg cgcatcaccg gcgcgagcgc gcacgatctc tccggcgttc gagcctgacg
     1921 cgggcgcgcg cctatttctg cggcagattg cgtagcaccg cggcgagctg atcgaggacg
     1981 ttgctcaagg cggtgttgaa cccgcgcacc agcgcatcgg ccgagcgtga agccacgtcg
     2041 atgcgcgagc cgagctcgcc gttccaaacg aaggtccccg acgcggcggc gctgctgctg
     2101 agaaagaact ttatcttaag caccgaggcc ggatgcgcgg ggtcgcgcaa gtcggcataa
     2161 agatcggtga cgaatgcttc gagggcgaag tcgccctcga gcgacgtgga tggcggcagc
     2221 acggcccggt agatgccggt cgccgcaagc caactcgcgg tcgcttcgcc gatcatcgtc
     2281 gccggcgaga ccaggaattc ctcgtagaag tcggcctcgt acttgagatc gctttcgcga
     2341 tagaccagcg tcctgccgcg aaacggcgcg gccacggcga ccgcttcgac cttgagcgtc
     2401 gccgcccccg gcgtcgcgct cttgttcgac ggtgccggcg gctcgagcag gtaggtcgac
     2461 ttgacaggcg atggccggga gagcgcgccg cacgcggcga tggtgagcgc cagtgcggac
     2521 gcgagcgccc gccggcgcga cagggagact gctgggttca tggctggagg cctttcgccg
     2581 gcggcggggg agcgccgagc agcagctgcg acgg
//
LOCUS       DATANS010000348         2966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_270024, whole genome shotgun sequence.
ACCESSION   DATANS010000348 DATANS010000000
VERSION     DATANS010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270024"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2172
                     /gene="gspD"
                     /locus_tag="VHT22_05035"
     CDS             <1..2172
                     /gene="gspD"
                     /locus_tag="VHT22_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEX3136950.1"
                     /translation="RGKRDTERYDGNDAKPNFPLSVSRPGQLLAAAAVAAILLAQPLR
                     AADDAVTLNFVNADIDAVIRAVGEMTGRNFVLDPRVKGVVNIVSATPVPRALIYPTLL
                     SALRLQGLAAIEGPGGVTKIVPEADAKQQGMPVVTGPVTVGGDRLVTAVYMLKYESAT
                     QLVNVLRPLITPNNAIAAVPTANALVITDYAENLKRIDRIIASLDVAPAGEPIVVPLR
                     NASALDIVPMLQRLLADTAPGAGAVADAQQRLTLVADPRSNSVLVRGENAARLARVRA
                     LIAELDTPGRAGGNISIIYLKNADAVRVARTLRALFSGGGDAGELTPAASLAPSAGSL
                     ASGGMTTATAAGAGATATVTASPVAGNAAGFAAAGVTIQADAANNALLVMASEPLYNN
                     IRAVVDKLDVRRAQIFVEALVVEVSADKAAEFGIQWNLLDPNKLTNNATQVGGGTNFG
                     TRGTGTNIIDAQSNLGALGQGLNLGIIRGTISIPGLGIITNLALLARALASDANTNIL
                     STPNLLTLDNEQANIIVAENVPFITGQYAQTGSTATVTPFQTIERRDVGLILRVKPQI
                     TEGGSVKLTIYEEVSRIEDTTNAAGIITNKRSLESTVMVDDGQIIVLGGLIQDSFTDG
                     TSKVPLVGDVPLFGQLFRYDNRRRIKTNLMVFLKPTVVRTSSAGASLTGDRYDYLIGE
                     QQRLAPGPRPFWPDPTEPRLPPITQPPAPMATPPAPPPGAK"
     gene            2193..>2966
                     /locus_tag="VHT22_05040"
     CDS             2193..>2966
                     /locus_tag="VHT22_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX3136951.1"
                     /translation="MPAPISTSSSGASAAPATLERVPYAFARMHGVLPLKDDGESVLV
                     LARSDASVEGIAELSRVLQRPLKTLTVNAERFAAELATAYNQAAPVTQFSGDLSRDAD
                     LARLLQDIPQAEDLLGATSDAPVIRMINALLLQALRERASDLHFEPYEARSVVRFRVD
                     GVLRDVVEPPRALHAALVSRLKVMASLDIAEKRLPQDGRMALKLGDKSVDVRVSTLPT
                     GPGERVVLRLLDKDTARLDLAALGMSEPTRDAIDALVREP"
BASE COUNT          498 a         1039 c          974 g          455 t
ORIGIN      
        1 cgcggcaagc gcgacacgga gcgatatgac ggcaacgacg ccaagcccaa cttcccgctc
       61 tccgtgagcc gaccgggcca attgcttgcc gcggccgcgg tcgcagccat cctgctggcc
      121 cagccgctcc gcgcagccga cgatgcggtt acgctcaatt tcgtcaacgc cgacatcgac
      181 gccgtcatcc gggcggtcgg cgaaatgacc ggccgaaact tcgtgctcga tccgcgggtg
      241 aagggtgtag tcaacatcgt ttccgcgacg ccggtcccgc gcgcgctgat ctacccgacg
      301 ctgctctcgg cgctgcgact gcaggggctg gccgcgatcg agggtcccgg cggcgttacc
      361 aagatcgtgc ccgaagccga cgccaagcag cagggcatgc cggtggttac cggtccggtc
      421 actgtcggcg gcgatcgcct ggtcaccgcc gtatacatgc tcaagtacga atcggcgact
      481 cagctggtca atgtgctgcg gccgctgatc accccgaaca atgcgatcgc cgccgtgccg
      541 accgccaacg cgctggtgat caccgactat gcggagaacc tgaaacgcat cgaccgaatc
      601 atcgcgtcgc tggacgtggc gcccgccggc gagcctatcg tcgtgccgct gcgcaatgcc
      661 tccgcgctcg acatcgtgcc gatgctgcag cggctgcttg ccgataccgc gcccggcgcc
      721 ggcgcggtgg cggatgcgca gcagcggctg accctggtcg ccgacccgcg ctccaacagc
      781 gtcctggtcc gcggcgagaa cgctgcgcgg ctcgcgcgcg tgcgcgccct gatcgcagag
      841 ctcgacacgc cgggacgcgc cggcggcaac atttcgatca tctatctgaa gaacgccgat
      901 gcagtgcgcg tggcgaggac gctgcgcgcg ctgttcagcg gcggcggtga cgcaggcgag
      961 ttgacgccgg cggcgtcgct ggcgcccagc gcaggcagcc tcgcctccgg tggaatgacg
     1021 actgctaccg cagccggtgc cggtgcgacg gctaccgtga cggcgtcacc ggtcgccggc
     1081 aatgctgccg gctttgccgc agccggcgtg accatacagg cagacgctgc caacaacgca
     1141 ttgctcgtga tggcgtccga acccttgtac aacaatattc gcgctgtcgt cgacaagctc
     1201 gatgttcgcc gcgcgcagat ctttgtcgaa gcgctggtcg tggaggtctc ggccgacaag
     1261 gccgcagaat tcggcattca gtggaatctg ctcgatccca acaagttgac caacaacgcg
     1321 acccaggtcg gcggtggcac gaacttcggc acgcgcggca ccggcaccaa catcatcgac
     1381 gcgcagtcga acctgggcgc gctggggcag gggctgaacc tcggcatcat ccgcggcacg
     1441 atcagcatcc ccggcctggg cataatcacc aatctcgcct tgctggcgcg cgctctcgcc
     1501 tccgacgcga acaccaacat actttcgacg ccgaacctgc tgacgctcga caacgagcag
     1561 gccaacatca tcgtcgccga gaatgtgccg ttcatcaccg gccagtatgc gcaaaccgga
     1621 tcgaccgcga cggtgacgcc gttccagacg atcgagcgca gggacgtcgg cctgatcctc
     1681 agggtcaagc cccagatcac cgagggcggc agcgtcaagc tgacgatcta tgaggaagtc
     1741 tcgcggatcg aggacacgac caacgccgcc ggcatcatta ccaacaagcg ctcgctcgag
     1801 tcgacggtga tggtcgacga cggccagatc atcgtgctgg gcggactgat ccaggactcg
     1861 ttcaccgacg ggacgagcaa ggtcccgctg gtcggcgacg ttccgctgtt cggccagctg
     1921 ttccgctacg acaaccggcg gcggatcaag accaacctga tggtgttcct caagccgacg
     1981 gtcgtccgca ccagcagcgc aggtgcatcg cttaccggcg atcgctacga ttacctgatc
     2041 ggtgagcagc agcgtctggc gcccgggccg cggccgttct ggcccgaccc gaccgagccg
     2101 cgactgccgc cgattaccca gcccccggcg ccgatggcga cgccgcccgc gccgccaccc
     2161 ggcgccaaat aggcagcaag gcccgcgggc ccatgccggc accgatctcc acttcctcct
     2221 ccggcgcgag cgccgcgccg gcaacgctcg agcgcgttcc gtatgcgttc gcgcgcatgc
     2281 atggcgtgct gccgctgaag gacgacggcg aatcggttct cgtgctggcg cgcagcgacg
     2341 catcggtcga aggcatcgcc gagcttagcc gcgtcctgca gcggccgctg aaaacgctga
     2401 cggtcaacgc cgagcgattc gccgccgagc tcgcgactgc gtacaaccag gcggcgccgg
     2461 tcacccagtt ttcgggcgac ctgtcgcgcg acgccgatct cgcgcggctg ctgcaggata
     2521 ttccgcaggc cgaggatctg ctcggcgcca cgtccgacgc gccggtgatc cggatgatca
     2581 acgccttgtt gctgcaggcg ctgcgcgagc gcgcgtcgga cttgcacttc gaaccgtacg
     2641 aagcgcgctc ggtcgtgcgc tttcgcgtcg acggcgtgtt gcgcgacgtc gtcgagccgc
     2701 cgcgtgcgct gcacgccgcg ctggtgtcgc ggctcaaggt gatggcgagc ctcgacatcg
     2761 ccgagaagcg tctgccgcag gacgggcgga tggcgctgaa gctcggcgac aagagcgtcg
     2821 atgtgcgcgt gtcgactttg ccgaccggcc cgggcgagcg cgtcgtgctg cgcctgctcg
     2881 acaaggatac ggcgcggctc gacctcgcgg cgctgggcat gagcgagccg acgcgcgacg
     2941 ctatcgacgc tctggtacgc gagccg
//
LOCUS       DATANS010000349         1830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_186844, whole genome shotgun sequence.
ACCESSION   DATANS010000349 DATANS010000000
VERSION     DATANS010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186844"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VHT22_05045"
     CDS             complement(<1..620)
                     /locus_tag="VHT22_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX3136952.1"
                     /translation="MSILALKIDVDTLRGTREGVPALLDLLRRHQVGATFLFSVGPDH
                     TGRAIKRIFRPGFFSKVRRTSVVQHYGVTTLLYGTALPGPDIGRRAGDTMLAVRDEGF
                     ETGLHCWDHVKWQDGVAGADADWTARQMRLACERYTEVFKEPPRVHGAAGWQMNVDAL
                     RQTQVLGFDYCSDGRGTHPHLPVWNAELIRCPQLPTTLPTLDELIG"
     gene            complement(682..1725)
                     /locus_tag="VHT22_05050"
     CDS             complement(682..1725)
                     /locus_tag="VHT22_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HEX3136953.1"
                     /translation="MKRILILGVNGFIGHHLSQRIIASTDWEVYGMDMQSERIADLLP
                     EKRFHFFEGDITINKEWIEYHIKKCNTVLPLVAIATPATYVREPLRVFELDFEANLPI
                     VRACVRYRKHIVFPSTSEVYGMCADAEFDSETSNLVLGPINKPRWIYACAKQLMDRVI
                     HAYGQQEGLDYTLFRPFNWIGSGLDSINTAKEGSSRVITQFLGHIVRGEPIKLVDGGT
                     QKRAFTYIDDGVSALMRIIANENGIASGKIYNVGNPLNNYSVRELARMMLDLALTYPE
                     YRDTAKQVELVDTTAEAYYGTGYQDVQNRVPDISNTCAELRWQPTVTMKESLKRIFDA
                     YRGQVAEARHLVD"
BASE COUNT          308 a          575 c          600 g          347 t
ORIGIN      
        1 atgccgatca gctcgtcgag cgtcggcagc gtcgtcggca gctgcgggca gcgtatcagc
       61 tcggcgttcc acaccgggag gtgcggatgc gtgccgcgac cgtcggagca atagtcgaac
      121 cccaggacct gcgtctgccg cagcgcatcg acgttcatct gccagcctgc ggcaccgtgc
      181 acgcgcggcg gctccttgaa cacctcggta tagcgctcac aggcgagccg catctgcctc
      241 gccgtccagt cggcatcggc gccggcgacg ccatcctgcc atttgacgtg atcccagcaa
      301 tgcagcccgg tttcgaagcc ttcgtcgcgc acagcgagca tcgtgtcgcc ggcgcgccgg
      361 ccaatgtcgg gtcccggcag cgccgtgcca tacagcagcg tcgtcacgcc gtagtgctga
      421 acgacggaag tgcggcgcac cttgctgaaa aatccgggcc ggaaaatgcg cttgatagcg
      481 cgcccggtgt ggtcgggccc gacgctgaac aggaaggtcg cgccgacctg gtggcggcgc
      541 agcagatcga gcagggccgg aacgccctcg cgcgtgccgc gcagcgtgtc gacgtcgatc
      601 ttcaacgcca ggatgctcat cgcggtttcg aatgcagtgc ggtgtcgacg cgctgccgtc
      661 gcgcccgaat cgcgtccatc gtcagtcgac caggtgccgt gcctcggcaa cctgcccgcg
      721 ataggcgtcg aatatgcgct tcagagattc cttcatcgtc accgtcggct gccaccggag
      781 ctcggcgcag gtattggaga tgtcggggac gcggttctgc acgtcctggt agcccgtgcc
      841 gtagtaggct tccgcggtcg tgtcgaccag ctcgacctgc ttcgccgtgt cgcggtattc
      901 gggataggtc agcgcgagat ccagcatcat ccgggccaac tccctgaccg aatagttgtt
      961 gagcgggttg ccgacgttgt agatcttgcc gcttgcaatc ccgttttcgt tggcgatgat
     1021 gcgcatcagc gccgatacgc cgtcgtcgat gtaggtgaac gcgcgtttct gcgtccctcc
     1081 gtcgaccagc ttgatcggct cgccgcgcac gatgtgcccg aggaactggg tgatcacgcg
     1141 cgagctgcct tccttggccg tattgatcga gtccaggccc gacccgatcc agttgaacgg
     1201 ccggaaaagc gtgtagtcga gcccttcctg ctggccgtag gcatggatca cgcggtccat
     1261 cagttgcttg gcgcaggcgt agatccagcg tggcttgttg atcgggccca gcaccaagtt
     1321 ggaagtctcg gaatcgaact cggcatcggc gcacatgccg tagacctccg aggtcgatgg
     1381 aaagacgatg tgcttcctgt agcgaacgca ggcgcggacg atcggcagat tggcttcgaa
     1441 gtcgagctcg aacacccgca gcggctcgcg cacgtaggtg gcgggagtgg cgatcgcgac
     1501 cagtggcagc accgtattgc acttcttgat gtgatattcg atccactcct tgttgatcgt
     1561 gatgtcaccc tcgaagaagt ggaagcgctt ttccggcagc aggtccgcga tgcgctcgct
     1621 ctgcatgtcc atgccgtaga cctcccagtc ggtcgaggcg atgatgcgct gcgacagatg
     1681 gtggccgatg aagccgttga cgccgaggat gaggatgcgt ttcatgcggg aaaggtcgcg
     1741 gccggagcgc gcgcattgta ccgcgcgacc gaatgacgtt cgtcagccca gcggccaggc
     1801 ggcgccgccg aatcgtgttc gcagcatcgc
//
LOCUS       DATANS010000350         1909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_648953, whole genome shotgun sequence.
ACCESSION   DATANS010000350 DATANS010000000
VERSION     DATANS010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648953"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VHT22_05055"
     CDS             <1..387
                     /locus_tag="VHT22_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX3136954.1"
                     /translation="RKREPELPESAIDKERGKPLRAPLIVVVGARVRDHKKVPAVEQI
                     VAAGAAAQNILVAAHALGFGGFWRTGNAAYDDGVKAALGLHVGDAIVGFLYLGTPAVP
                     APPPASSNHAAQVAHWTGPPDHDAGP"
     gene            464..1468
                     /locus_tag="VHT22_05060"
     CDS             464..1468
                     /locus_tag="VHT22_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3136955.1"
                     /translation="MKIRIIGAWLAVLCMMAGSADAQAPARPLAPVRVISFDGGWNLA
                     VWAAQRQGFFEANGVSVQLAYTPSSGFLIASLFDGKQDIALALMDNLIAYQEGQGEVK
                     IAESPDLVAILGGDGGFLSVVAAPGVKSFADLKGKTLSVDALSTGAAFVLRELVAKNG
                     LTDDDVKYVRAGGTSNRYVELLAGKHDATLLRTPFELLAQNRGYRVLASADMLGAYQG
                     TAGFVRRSWAEAHEAAVLGFLKGYRAGVDWIYQPGNREIVEALLVANIRDMTPALARQ
                     SYDLLLAQPGGIYPDARPNVAGIGTVLALRSKYATPPKILNDPARYIDLRFYDKAFPR
                     "
     gene            1492..>1909
                     /locus_tag="VHT22_05065"
     CDS             1492..>1909
                     /locus_tag="VHT22_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEX3136956.1"
                     /translation="MAKKKRSDPKIVRPDDIDPSYRWDRPLQAPGHTQVDFEERVNFR
                     RLHDYRLARTRAALAGSGLGALLCFDQHNIRYISSTVIGEWARDKLIRYCLLSGNGEP
                     WVWDFGSAARHHRLHAPWLDPTHCIAGMVGMRGAVSP"
BASE COUNT          319 a          645 c          625 g          320 t
ORIGIN      
        1 cgcaagcgcg agccggaact gcccgaatcg gcgatagaca aggagcgcgg caagccgctg
       61 cgagcgccgc tgatcgtcgt cgtcggggcg cgggtgcgcg atcacaaaaa agtgcctgcc
      121 gtcgagcaga tcgtggcggc gggcgccgcc gcgcagaata tcctggtggc cgcgcatgcg
      181 ctgggcttcg gcggcttctg gcgcaccggc aatgctgcct acgacgacgg cgtcaaggcc
      241 gcgctgggcc tgcacgtggg cgatgcgatc gtcggattcc tttatctggg cacgccggcc
      301 gtgcccgccc cgccgcccgc atcgtccaat catgccgcgc aggtagcgca ctggaccggg
      361 ccgcccgatc acgatgccgg accttgagtg cgccggcgat cctaagttat cgtccgactg
      421 ctgcacgaac cctccggacc cgacaacacc cagataaatg cccatgaaga ttcgcatcat
      481 cggcgcatgg cttgcggtgt tgtgcatgat ggccggatcg gccgacgcgc aggcccccgc
      541 ccgtccgttg gcgccggtgc gcgtcatcag cttcgacggg ggctggaacc ttgcggtctg
      601 ggcggcgcag cggcagggat ttttcgaggc gaacggcgtc tcggtgcagc tcgcctatac
      661 gccgagctcc ggttttctga tcgcttcgct gttcgacggc aagcaggaca tcgcgctggc
      721 gctgatggac aacctgatcg cctatcagga aggccagggc gaggtcaaaa tcgccgagtc
      781 acccgacctc gttgcaatcc tcgggggtga cggcggattc ctgtcggtgg tggctgctcc
      841 tggcgtcaaa tcgttcgccg atctcaaggg caagacgctg tcggtcgacg ccctctccac
      901 cggcgccgcg ttcgtcctgc gcgagctggt cgcgaaaaac ggcctcaccg acgacgacgt
      961 caaatacgtg cgcgccggcg ggacgtccaa tcggtacgtc gagcttctcg ccggcaagca
     1021 cgatgccacc ctgctgcgta cgccgttcga attgctggcg cagaatcgcg gttaccgcgt
     1081 tctggccagc gcagacatgc ttggggccta ccaaggcacc gccgggttcg tccgacgctc
     1141 gtgggccgag gcgcacgagg cagcggtgct cggttttctc aagggatatc gcgccggcgt
     1201 cgattggatc taccagcccg gcaaccggga aatagtcgag gcactgctgg tcgccaatat
     1261 ccgcgacatg acgccggcgc tcgcgcggca gtcgtacgat ctcctgctgg cgcaacccgg
     1321 cggtatctat ccggacgcgc ggcccaacgt ggccggcatc ggcacggtgc tcgcgctgcg
     1381 tagcaagtat gcgacgccgc cgaagatctt gaacgaccca gcgcgctaca tcgacctccg
     1441 gttctacgac aaggctttcc cccgctgaca acgaccggaa gatgtcaccg catggctaag
     1501 aaaaaacgta gcgaccccaa gatcgtccgg cccgatgaca tcgatccctc ctaccgctgg
     1561 gatcgtccgt tgcaggcgcc cggacacacg caggtcgact tcgaggagag ggtcaatttc
     1621 cgccggctgc acgactaccg gctcgcccgc acgcgcgcgg cactggccgg atcggggctc
     1681 ggtgccttgc tgtgcttcga tcagcacaat atccgctaca tctcgagcac ggtgatcggc
     1741 gagtgggcgc gcgacaagct catccgctac tgcctcctca gcggcaacgg ggagccctgg
     1801 gtgtgggact tcggctcggc cgcgcgccac catcggctgc acgcgccctg gctcgatccc
     1861 acccactgca tcgcgggcat ggtgggaatg cgcggggcgg tgtcgcccc
//
LOCUS       DATANS010000351         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_649065, whole genome shotgun sequence.
ACCESSION   DATANS010000351 DATANS010000000
VERSION     DATANS010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649065"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2135)
                     /locus_tag="VHT22_05070"
     CDS             complement(<1..>2135)
                     /locus_tag="VHT22_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3136957.1"
                     /translation="MSAGGANIDHAIGETPNIAARIQAEASPNSVCISAATLKLVGER
                     FHVRALGARPLKGVAIDTEVYSVVGAIDSKSELHGGHQGAPLVGREMELRHLLERWDL
                     AQRGRGQAVLLSGEGGIGKSRLLTALRERASAGSTAWRNIHCSPFYQNSALHPIIDLI
                     DRAIDAAGATPGSDRYAVLRRILGASGITDDTTHALIGSLMGLSDEHQQALSDLAPAQ
                     RKRRTLDALIAWLHADAKKYPLVVVVEDLHWVDASTRELLGMLLERIVEVPMLVVLTF
                     RPEFVPTWAMHGQISMIALNKLLPEQVVTVARGITGGKALPARIVDEIVRRTDGVPLF
                     VEELTKAILASGLVVERDGELAFSGSQSAQLEVPATLRDSLTARLDRLGAAKAVAQLA
                     SVLGRAFEYPVLQSVCDLPGAELEEQLAALNRADIIQQSGVPPQSHYVFKHALIQEAA
                     YDTLLKSVRQRHHQRIAQCYVDRFADVAQSRPELVAHHYSRAMMPAAAVPFWQKAGEL
                     AVARTGYEEAIAHLGAALEQIQLVPDSQARAATELSLRVTIGPALIAGKGMAAPDTGA
                     NYARACELAETLGDTKERFAALWGDWLYKNGTGQLIEGARRSEDLVMLSRKLGDDEYA
                     LQAHHSRWTNFFFLGDVTVARADSLRGIELYDRVRHRHHKHLYGGHDPGVCAYNFGSN
                     AAWATGHSKEALQLAEKSMALAKELDHSL"
BASE COUNT          348 a          713 c          691 g          383 t
ORIGIN      
        1 ctgagcgagt gatccagctc tttcgcgagc gccatcgatt tctcggcaag ctggagcgcc
       61 tccttgctgt gaccggtcgc ccatgcggcg ttcgagccga aattgtaggc gcagacgccg
      121 ggatcgtggc cgccgtacag atgcttgtgg tggcggtgcc gcacgcggtc atagagctcg
      181 atcccgcgca gagaatctgc gcgtgccacg gtgacatcgc cgaggaagaa aaaattggtc
      241 cagcgggagt gatgcgcctg cagcgcgtat tcgtcgtccc ccagcttgcg gctgagcatg
      301 acgaggtcct cggaacggcg tgcgccctcg ataagctgtc ccgtgccatt cttgtatagc
      361 caatcgcccc acaatgcggc gaagcgctcc ttggtgtcgc ccagcgtttc agccagctcg
      421 caggcgcggg catagttggc gccggtatcc ggcgccgcca tgcctttgcc ggcgatcagc
      481 gccgggccta tggttacgcg cagactcaac tcggtagcgg cgcgcgcttg cgagtcgggc
      541 acgagctgga tctgctccag tgccgcgccg aggtgcgcga tcgcttcctc gtagccggtg
      601 cgggccacgg caagctctcc ggccttctgc cagaagggca ccgcagcggc cggcatcatc
      661 gcgcggctgt aatggtgcgc caccagctcc ggtcgcgatt gcgccacgtc cgcaaagcga
      721 tcgacgtagc attgtgcgat ccgttgatga tgccgctggc gcacgctttt cagaagggtg
      781 tcgtaggctg cctcctggat cagcgcgtgc ttgaagacgt agtgtgattg ggggggtacc
      841 ccgctctgct ggatgatgtc cgcgcgattc agcgcggcca gctgctcctc gagctcggct
      901 ccgggaagat cgcacaccga ctgcaagacc gggtactcga acgcgcggcc gagtacggag
      961 gcaagttgcg cgaccgcctt ggcggctccg agccgatcca gccgtgcggt gagcgagtcg
     1021 cgcagcgtcg ccggcacctc gagttgcgcc gactgactac ccgagaacgc cagctcacca
     1081 tcgcgttcga cgaccagccc ggacgccaat atcgccttgg tcagctcctc gacgaacagc
     1141 ggcacgccgt cggtgcggcg cacgatctcg tcgacgatgc gcgcgggcag cgctttaccg
     1201 ccggtaattc cccgggccac cgtcacgacc tgctccggca agagcttgtt cagcgcgatc
     1261 atcgagatct gcccgtgcat cgcccaggtc ggaacgaact cgggccggaa agtgagcacg
     1321 acgagcatcg gtacttcgac aatgcgctcc agcagcatcc cgagcaattc gcgcgtcgac
     1381 gcatcgaccc aatgcaggtc ttcgacgacc accaccagcg gatacttttt cgcatcggcg
     1441 tgcaaccacg caatcagcgc gtcgagcgtc cgccgcttgc gctgggcagg cgccaggtcg
     1501 ctcagtgcct gctggtgctc gtccgaaagc cccatcagtg atccgataag ggcgtgcgta
     1561 gtgtcgtcgg tgatgcccga ggcgccgagg atccgtcgca gcaccgcata gcggtcggac
     1621 cccggcgtcg cccccgcggc gtcgatcgcc cggtcgatga gatcgataat cggatggagc
     1681 gcgctgttct ggtagaacgg cgagcaatgg atgttgcgcc acgccgtgct gcctgcggaa
     1741 gcgcgttcgc gcaatgcggt caacagccgg gacttgccga tgcccccctc tccggaaagc
     1801 agcaccgcct gtccccgtcc ccgctgcgcc agatcccagc gctcgagcag atgtcgaagc
     1861 tccatttcgc ggccgacgag tggcgcgccc tggtggcctc cgtgcagctc cgacttggag
     1921 tcgatcgcac cgacgaccga gtagacctcg gtatcgatcg ccaccccttt caacggccgc
     1981 gcgcccaagg cgcggacgtg gaagcgttcc ccgacgagtt tgagcgtcgc ggcgcttatg
     2041 cataccgaat tcgggcttgc ttccgcctgg atccgtgcgg cgatgttcgg tgtttccccg
     2101 atggcgtggt cgatgtttgc tccgccggcg ctcat
//
LOCUS       DATANS010000352         1975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_479923, whole genome shotgun sequence.
ACCESSION   DATANS010000352 DATANS010000000
VERSION     DATANS010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479923"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..606
                     /gene="secB"
                     /locus_tag="VHT22_05075"
     CDS             136..606
                     /gene="secB"
                     /locus_tag="VHT22_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEX3136958.1"
                     /translation="MADTQAQPTPSLSIEKIYVKDLSLENPGAPQSFTTPEAPQVEVG
                     LRTRGEQIEENVYECVLTITVTAKTADKTLFLVEAAQAGIFRIVGVPLSDIQPLMGIH
                     CPTVLFPYIRETIADAVSRAGFPPVHLDPINFEALYQQQIASMQQQAPAVPATN"
     gene            613..1062
                     /locus_tag="VHT22_05080"
     CDS             613..1062
                     /locus_tag="VHT22_05080"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX3136959.1"
                     /translation="MGRVSSLLVATIALSGGAGAADFQSASGAAVLYDAPSLKAKPLF
                     VIGRDTPFEVIVGVEGWLKIRDASGTVAWIERKAVSERRTLIVRVPVADVLATPEGNA
                     PLVFKVEQNVVLELIDPAYVSNTPGWAKVRHRDGQQGYIRIAQVWGL"
     gene            1084..>1975
                     /locus_tag="VHT22_05085"
     CDS             1084..>1975
                     /locus_tag="VHT22_05085"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX3136960.1"
                     /translation="MSDTVAIVGAGAWGTALAVHLAGVAAPPALRLWARDSTAARAIA
                     ASRENSRYLPGIAIPSEIDVDFELSRAIAGASLIIVATPIGALTGIVQSLRTQTKAPL
                     AWLGKGFVAAAEAPGVALAHRVIAPMWPAPVGVISGPSFAEEVARRLPTAISVAATEP
                     DFAQSIAQRLRGDTLRAYVSDDLTGTEVGGAVKNVLAIAAGASDGLGFGNNARAALIT
                     RGLAETARLAVALGGRRETLTGLSGLGDLVLTCTGDLSRNRRVGLGLGRGESLAAILA
                     ALGHVAEGVSAARAVHTLAAA"
BASE COUNT          311 a          659 c          686 g          319 t
ORIGIN      
        1 cacgtcggcg gatacgacga cctggtcgtg ctcgaccgcg agggcgggct tctgccgctg
       61 ctcgccgcgg acccgcaggc gggcgctgca accggcgcta cgctataatc gtacgtttac
      121 cgccggggaa atcgcatggc cgacacccaa gctcaaccga cgccgtcgct gtcgatcgag
      181 aagatttacg tcaaggatct ttcgctcgag aatccgggcg cgccgcagtc gtttaccaca
      241 cccgaggcgc cgcaggtcga ggtcggactg cgcacgcgcg gcgagcagat cgaggaaaac
      301 gtctacgaat gcgtgctgac gatcacggtg accgcgaaga cagcggacaa gacgctgttc
      361 ctggtcgaag ccgcgcaagc gggcatcttc cgcatcgtcg gggtcccgct ttccgatatt
      421 caaccgctaa tgggcattca ttgcccgacc gtgctgtttc cctacatccg cgagacgatc
      481 gccgatgcgg tttcacgcgc cggctttccg ccagtccacc tggacccgat caatttcgaa
      541 gcgctgtacc agcagcagat cgccagcatg caacagcagg cgcccgcggt accggcgacg
      601 aactagcggg cgatgggacg cgttagctcg ctgctggtcg cgacgatcgc gctcagcggc
      661 ggcgcggggg cggcggactt ccagtcggcg agcggagcag cggtgctcta tgacgcgccg
      721 tcgctcaagg ccaagccgct tttcgtcatc ggccgcgaca caccgttcga agtcatcgta
      781 ggcgtcgaag gctggctcaa gatccgcgac gccagcggca ccgttgcgtg gatcgagcgc
      841 aaggcggtgt cggagcggcg cacgttgatc gtgcgcgtcc cggttgccga tgtgctcgcc
      901 acgcccgaag gcaatgcgcc gctggtattc aaggtcgagc agaacgtcgt acttgagctc
      961 atcgacccgg cgtatgtcag caatacgccg gggtgggcga aggtgcgtca ccgcgacgga
     1021 cagcagggct acattcgcat cgcccaggtc tggggcctgt agcagcgtcg tgcagtcgta
     1081 ccgatgagcg acaccgttgc gatcgtcggc gccggcgcct ggggaacggc gctggccgtg
     1141 catctggccg gcgtcgccgc accgccggcg ctgcgtcttt gggcgcgcga ttccacggca
     1201 gcgcgcgcga tcgcggcctc gcgcgagaat tcgcggtacc tgcccggcat cgccattccg
     1261 agtgagatcg acgtcgattt cgaactgtcg cgcgcgattg ccggcgcttc gctgatcatc
     1321 gtggccacgc cgatcggtgc gctgaccggt atcgtgcagt ccctgcgcac gcaaaccaag
     1381 gcgccgctcg cctggctggg caagggcttc gtcgcagccg ccgaggcgcc gggcgtcgcg
     1441 ctggcacatc gtgtcatcgc gccgatgtgg ccggcgccgg tcggcgtaat ctccggtccg
     1501 agctttgccg aggaagtcgc gcgccggctg ccgacggcca tcagcgtcgc ggccactgag
     1561 cccgatttcg cgcaatcgat cgcccagcgg ctgcgcggcg acacgttgcg cgcctacgtc
     1621 agcgacgact tgaccggcac cgaagtcggc ggagcggtga aaaacgtgct ggcgatcgcc
     1681 gccggcgcga gcgacgggct gggttttggc aacaatgcgc gcgccgcact gatcacgcgc
     1741 ggtctggccg aaacagccag gctcgcggtg gcgctgggag gcaggcgcga gaccctgacc
     1801 gggctttccg gcctcggcga cctagtgctg acttgcaccg gcgacctgtc gcgaaaccgg
     1861 cgcgtgggac tcgggctcgg gcgcggcgaa tcgctcgccg cgattcttgc ggcgctgggc
     1921 catgtcgccg agggtgtgtc ggccgcgcgg gcggtgcaca cgctggccgc cgcgc
//
LOCUS       DATANS010000353         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_522795, whole genome shotgun sequence.
ACCESSION   DATANS010000353 DATANS010000000
VERSION     DATANS010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522795"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VHT22_05090"
     CDS             complement(<1..331)
                     /locus_tag="VHT22_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3136961.1"
                     /translation="MPVLGLGTWRMGERRDRREREIAAVKAGIDLGVTLIDTAEMYGE
                     GEAEKIVGEAIAGRRERMFLVSKVYPHNASRRGAVAACKRSLKRLGTDHLDLYLLHWR
                     GDVPLAET"
     gene            483..>1554
                     /locus_tag="VHT22_05095"
     CDS             483..>1554
                     /locus_tag="VHT22_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX3136962.1"
                     /translation="MPLDRSAKQPPGHTIVRAACPHDCPDTCALHVTVAEGRAIKVEG
                     DPDHPTTRGVLCTKVARYLDRTYSEKRVLHPLRRVGAKGEGRFERIDWDEALDTIASR
                     FREIAESSDGPQAILPYSYAGTMGLLQYGSMDRRFFNKLGASLLDRTICATAGKAGYA
                     ATIGASIGTDLEQFENARLILIWGSNPVVSNLHLWSRVQEAKRRGARLIAIDPYRSQT
                     AEKCHEHLALMPGTDAALALGIMHVLIAEGLIDRDYIDRYTVGFDALEQRVRDYPPSR
                     VASLTGLREAQIVALAREYGTIEPAVIRLNYGMQRHAGGGMAIRTVACLPALVGAWRH
                     AAGGALLSSSGTYPINTAALERP"
BASE COUNT          277 a          540 c          485 g          252 t
ORIGIN      
        1 cagtttccgc aagaggcacg tcgccgcgcc agtgcagcag atacagatcg aggtggtcgg
       61 tgcccaggcg cttgagactg cgtttgcacg cggcgaccgc gccgcggcgg cttgcgttgt
      121 gcgggtagac tttgctcacc agaaacatcc gctcgcgccg tcccgcgatc gcctcaccga
      181 cgattttttc ggcttcacct tcgccgtaca tctccgcagt gtcgatcagc gtcaccccga
      241 gatcgatgcc ggccttgacc gcagcaatct cccgctcgcg ccgatcgcgg cgctcgccca
      301 tgcgccaggt gcccaagccc agcacgggca tcgccgcgcc cgagcgcagc gcgatcgtcg
      361 gaagagttgc cgccacggta ggcctttcgc tggattcgcc tgtacgagac gatagcgcaa
      421 ccattgggca cgggcgaggc ccgcccggcc ggattacaat acgaccatcc cccggatccg
      481 cgatgccact cgaccgttca gccaaacaac cgcccgggca cacgatcgtc cgcgccgcgt
      541 gcccgcacga ttgtcccgac acctgcgcgc tgcacgtgac ggtagccgag gggcgcgcga
      601 tcaaggtcga aggtgatccc gatcacccga cgacgcgcgg cgtgctctgc accaaggtcg
      661 cccgctatct ggaccgaacg tattcggaaa agcgcgtgct gcatccgctg cgcagagtcg
      721 gcgccaaggg cgaaggccgc ttcgagcgca tcgactggga cgaggcgctc gacacgattg
      781 cgtcgcgctt cagggaaatc gccgagtcat ccgatggccc gcaggcgatt cttccctaca
      841 gctatgccgg aacgatggga ttgctgcaat acggctcgat ggaccggcgc ttttttaaca
      901 agctcggcgc gtctttgctc gatcgcacga tctgcgcgac cgccggcaag gcaggatatg
      961 ccgcgacgat aggcgcatcg ataggcaccg acctcgagca attcgaaaat gccaggctga
     1021 tcctgatctg gggttcgaac cccgtcgtat cgaaccttca cctctggagc cgcgtgcagg
     1081 aagccaagcg ccgcggcgcc aggctgatcg caatcgatcc gtatcgcagc cagaccgcag
     1141 agaaatgcca cgagcacctg gcgctgatgc cgggcaccga cgctgcgctc gcgctcggca
     1201 tcatgcacgt cctgatcgcc gagggcctga tcgatcgcga ttacatcgac cgctacacgg
     1261 tgggattcga tgcgctggaa caacgcgtgc gcgactaccc gccgtcgcgc gtcgcttcgc
     1321 tcaccggatt gcgcgaagcg cagatcgtcg cgctggctcg cgagtacggc accatcgagc
     1381 ccgccgtgat ccgcctgaac tacggcatgc aaaggcatgc cggcggcggc atggcgatcc
     1441 gcacggtcgc atgtctgcct gcgctggtcg gcgcatggcg ccacgccgcc ggcggcgcgc
     1501 ttctatcgtc cagcggcacg tatccgatca acacggcggc gctcgagcga ccgg
//
LOCUS       DATANS010000354         1951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_649303, whole genome shotgun sequence.
ACCESSION   DATANS010000354 DATANS010000000
VERSION     DATANS010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649303"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VHT22_05100"
     CDS             <1..598
                     /locus_tag="VHT22_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX3136963.1"
                     /translation="GITLFLIDAKASGVNITRTPTVDSHNASAVELRDVAVSDTDVVG
                     TFDKGAVVLDAVLDAGRAVLAAELLGVAEESFERTLTYLREREQFGAKIGTFQALQHR
                     AAHLFCEIELVRSVVLRTLQALDAQEPTASNLVCLAKGKASDVARIATNEAVQMHGGI
                     GMTDEFDIGFFMKRARAAGETFGDYYYHADRYAQLAGY"
     gene            692..940
                     /locus_tag="VHT22_05105"
     CDS             692..940
                     /locus_tag="VHT22_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136964.1"
                     /translation="MYLTGADLIDMTLKQLEMLEEILRADARAYAEERDAAARDARHC
                     REATDAIRALIPRLLAARAAQLPKSQLQIPSCANDDEL"
     gene            1065..1340
                     /locus_tag="VHT22_05110"
     CDS             1065..1340
                     /locus_tag="VHT22_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136965.1"
                     /translation="MTTERDPDTEPTVEIERPNLQAPAAESEDVDQDFDPEKTLVRDD
                     WESTAIRRVAPHVAAVQDEVAADDQEPFGWESEAVRRLSRVVQNNQI"
     gene            complement(1337..>1951)
                     /locus_tag="VHT22_05115"
     CDS             complement(1337..>1951)
                     /locus_tag="VHT22_05115"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX3136966.1"
                     /translation="GSELRVAPAKSGREVEVTIWKDSCAAFDAGDEPAEFLSSYLGSP
                     VRLVRFDARRKRASDQQWTSGVEALNQFSDAFPWLLASEASLDDLNSRLQNKLPMNRF
                     RPNIVVSGLPPFGEDQLHDLTAGKVRLRRVKGCTRCIVTTTDQATGLRDGKEPLQALA
                     KFRFDRELKGVIFGQNLILIEGAGAQLRVGQELTPAPGRLDPAI"
BASE COUNT          376 a          616 c          632 g          327 t
ORIGIN      
        1 tggcatcacg ctgttcctga tcgacgccaa ggcaagcggc gtcaacatca cgcgcacacc
       61 gactgtcgac agtcacaatg cgtcggcggt cgagttgcgc gatgtggccg tgagtgacac
      121 cgacgttgtc ggcacgttcg acaagggtgc agtcgtactc gatgcggttc tcgacgcggg
      181 tcgcgcagtg cttgcggccg agttgctcgg cgtggcggag gaatccttcg agcgcacgct
      241 gacctatctg cgcgagcgcg aacagttcgg cgcgaagatc ggcacgttcc aggcactgca
      301 gcaccgtgcg gcgcatttgt tctgcgagat cgagctggtg cggtcggtcg tgctgcgcac
      361 attgcaggcg ttggatgcgc aggagccgac ggcttcgaat ctcgtgtgcc tcgccaaggg
      421 caaggcaagc gacgtggcgc ggatcgcgac caacgaagcg gtgcagatgc acggcggcat
      481 cggcatgacc gacgaattcg acatcggctt cttcatgaag cgcgcccgcg ccgccggcga
      541 gactttcggc gactactact atcacgccga tcgctacgcg cagctcgcgg ggtactgacg
      601 cctgaatttc ctgcagtttc cgcttctgcg ctttcgcgcg caccgccaac tgtatataac
      661 tacaaccctg gacctgggcc aggaaccgca catgtatctc accggcgccg atctgatcga
      721 catgacgctc aagcaactcg agatgctcga ggagattctg cgcgcggacg cacgcgcgta
      781 tgccgaggag cgcgacgcgg cggcacgcga cgcgcgtcat tgccgagaag ccaccgacgc
      841 tattcgcgcg ttgattccaa gactgctcgc cgcgcgcgcg gcacagctgc cgaaaagtca
      901 attgcagatt cccagttgtg caaacgacga cgagttgtaa cgccgctacg gcgcggaact
      961 tcgtcacacg acagaacgtt cagcaccgaa gcgtgatcca cgtcctcacc ggtcgcggcc
     1021 ggccgcgagt acgttgggcg cactgggaag aggcgacagg acttatgaca acagagcgcg
     1081 atccggacac cgaaccgacg gtcgagatcg agcggccgaa tctgcaggca cccgcggcgg
     1141 aaagcgaaga cgtcgatcag gacttcgatc cggaaaagac gctggtgcgc gacgattggg
     1201 agagcacggc gatccggcgc gtcgcgccgc acgtcgcggc cgtgcaggac gaagtcgctg
     1261 cggacgacca ggagccgttc ggctgggaaa gcgaggccgt gcgcaggctg tcgcgcgtgg
     1321 tccagaacaa tcagatctag atggccggat cgagtcgacc cggggcagga gtcagttctt
     1381 gcccgacgcg cagctgcgcg ccggcgcctt cgatcagaat caggttctga ccgaagatca
     1441 ctcccttcaa ttcacgatcg aaacgaaatt tcgccagcgc ctgcagcggt tctttaccgt
     1501 cgcgcaggcc ggtggcctgg tcggtcgtcg tgacgatgca acgcgtgcag cctttcacgc
     1561 ggcgtagccg gaccttgccc gccgtcagat cgtgcagctg atcttcgccg aacggcggca
     1621 agccgctgac gacgatgttc gggcggaagc gattcatcgg gagcttgttc tgcagccggc
     1681 tgttgaggtc gtcgagcgac gcctcggaag ccagcagcca cgggaaggcg tcggagaact
     1741 gattcagcgc ttccacaccg gaggtccact gctgatcgct ggcgcgcttg cgacgcgcat
     1801 cgaaacgtac cagccgcacc ggcgagccga gatagctgct caggaactcc gcgggctcgt
     1861 cacctgcgtc gaaagccgcg cagctgtctt tccagatcgt aacctcgact tcgcgcccgg
     1921 acttcgcggg cgccacacgc aactctgagc c
//
LOCUS       DATANS010000355         2235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_227650, whole genome shotgun sequence.
ACCESSION   DATANS010000355 DATANS010000000
VERSION     DATANS010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227650"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VHT22_05120"
     CDS             complement(<1..404)
                     /locus_tag="VHT22_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjP-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HEX3136967.1"
                     /translation="MTGGRPDAGEGSLRRVMVAGQDVDYRLFRARRSSIGMVIDHNGL
                     VVRAPRWVALREIELALIERADWVIKTLAEWRGKNRDSLPTEWRAGAALLYQGRPLTL
                     ALFPARRKAIAADLLHLTVLHPDPGDETEIAA"
     gene            complement(401..1144)
                     /locus_tag="VHT22_05125"
     CDS             complement(401..1144)
                     /locus_tag="VHT22_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX3136968.1"
                     /translation="MSALRSLLFALYQMILTPIYATLVLLLFWLPPLPRFRFITGWCL
                     LNLIGARLICGIRHRVIGAENIPPRADPHLVMSKHSSTWETLSLNFLFPPLAFVAKKE
                     LLSIPFFGWAFTLASPITIDRQAGHDAMSQLVTQGRERLRQGFWIVVYPEGTRIRAGT
                     RAHYKSGGARLAIALGIPIVPVAHNAGYLWPKGVLGKRAGTVTVSIGPPIRPVGDDMQ
                     RLIGEVESWIENEVARLGDPLNERGNARR"
     gene            complement(1165..1722)
                     /gene="gmhB"
                     /locus_tag="VHT22_05130"
     CDS             complement(1165..1722)
                     /gene="gmhB"
                     /locus_tag="VHT22_05130"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEX3136969.1"
                     /translation="MVPTRQAKLIVLDRDGVINYDSDQFIKSPDEWRPIPGSLEAISR
                     LNHAGFRVIVATNQSGLGRRLFDMATLISIHDKMYKALAQLGGRVDALFYCPHTADSN
                     CDCRKPKPGMLVEIGQRFAVDLTGVPCVGDGERDLQAAAAVGAQPILVLTGKGEKTLR
                     SGNFPQDTAIFPDLAFFVTALLAGD"
     gene            complement(1753..>2235)
                     /locus_tag="VHT22_05135"
     CDS             complement(1753..>2235)
                     /locus_tag="VHT22_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HEX3136970.1"
                     /translation="RLRGYLRDRGYTANQVAAVVDSRPDCIDDLPERLEAVRAFEALP
                     EALSLSAANKRIVNILRKAQGEGGGAVDLSVLVDGAERALHAAFDQLRPIVASRVADG
                     DFSAALRALASTKPTVDRFFDDVMVMADDPKIRGNRLALLREVSATMNRVADISKLAV
                     "
BASE COUNT          397 a          746 c          737 g          355 t
ORIGIN      
        1 aatgccgcga tctcggtttc gtcgccaggg tccggatgca gcaccgtcag gtgcaagaga
       61 tcggcggcga tcgccttgcg gcgcgccggg aacagcgcca gcgtcagcgg gcggccctgg
      121 tacagcagcg ctgcgcccgc gcgccattcg gtaggcagcg agtcgcggtt cttgccccgc
      181 cattcggcga gcgtcttgat cacccagtcg gcgcgctcga tcagcgccag ctcgatctcg
      241 cgcaatgcga cccagcgcgg cgcgcgcacg acgagtccgt tgtggtcgat caccatgccg
      301 atcgaactgc ggcgagcacg gaaaaggcga tagtcgacgt cctggccggc gaccatgacg
      361 cgacgcaggc tgccttcgcc cgcgtcggga cggccaccgg tcaacggcgc gcgtttccgc
      421 gctcgttcag cggatcgccc agccgcgcga cttcgttctc tatccaggac tcgacctccc
      481 cgatcagccg ctgcatgtca tcgcctacgg gacggatcgg cggaccgatc gacacggtca
      541 ccgtgcccgc ccgcttgcca agcacgccct taggccacaa atagccggca ttatgcgcca
      601 caggaacgat cgggatgcca agcgcgatcg ccaggcgtgc gccgccgctc ttgtagtgcg
      661 cgcgcgtccc ggccctgatt cgcgtgcctt ccgggtagac gacgatccag aatccctggc
      721 gcaggcgctc gcgtccctgc gtgacaagct gactcatcgc atcgtgtccc gcctgccgat
      781 cgatcgttat cggggacgcc agggtgaacg cccagccgaa gaatggaatc gacaacagct
      841 ccttcttggc gacgaaggcg agcggaggga acagaaagtt gagcgacagc gtttcccacg
      901 tcgacgagtg cttcgacatc accaggtgcg gatcggcccg cggcgggatg ttctcggcgc
      961 caatgacgcg gtgccggatg ccgcagatca gcctggcgcc gatcagattc agcagacacc
     1021 agccggtaat gaaccggaag cgaggcagcg gcggcagcca gaacaaaagc aggaccagcg
     1081 tcgcatagat cggcgtcagg atcatctgat acagcgcgaa cagcagcgag cgcagcgccg
     1141 acatcgtgga cgcctcgagc gacttcagtc gcccgcgagg agcgcggtca cgaaaaaagc
     1201 gaggtcggga aagatcgccg tatcctgcgg gaaattgccg ctgcgcagcg ttttctcgcc
     1261 cttcccggta agaaccagga tcggctgcgc gccaacggct gccgcggcct gcagatcgcg
     1321 ctccccgtcg ccgacgcaag gcaccccggt caggtcgacc gcgaagcgtt gtccgatctc
     1381 gaccagcatg cccggcttgg gcttgcggca atcgcaattc gagtccgccg tatgcgggca
     1441 ataaaacagc gcatcgactc tgccgccgag ctgggcgagc gccttgtaca tcttgtcgtg
     1501 gatcgagatc agcgtggcca tgtcgaacag gcgtcggccc agacccgact ggttggtcgc
     1561 gacgatcacc cggaatccgg cgtggttgag ccgcgagatc gcctccaggc tgcccgggat
     1621 cggccgccat tcgtcgggcg acttgatgaa ctggtcgctg tcgtagttga tgacgccatc
     1681 gcgatccagg acgatcagct tagcttgtcg ggtcggcacc atgcgttccg tcgagggcgg
     1741 ggaatcgcgt cgtcagacgg ccagcttgga aatgtcggcg acgcggttca tcgtcgccga
     1801 gacttcgcgc aagagcgcca ggcggttgcc gcggatcttc gggtcgtcgg ccatgaccat
     1861 cacgtcgtcg aagaagcggt cgaccgtcgg cttggtcgag gccagcgccc gcagcgcggc
     1921 tgagaagtcg ccgtcggcga cgcggcttgc gacgatcggc cgcagctggt cgaatgccgc
     1981 gtgcagcgct cgctcggcac cgtcgacaag caccgaaaga tcgacggcgc cgccgccttc
     2041 cccctgcgcc ttgcgcagta tgttgacgat gcgcttgttg gcggcagaaa gcgacagcgc
     2101 ttcgggcagc gcctcgaatg cgcgcaccgc ctccagccgc tcgggcagat cgtcgatgca
     2161 atcgggccgg gaatcgacga cggccgcgac ctggttcgcc gtgtatcctc ggtcgcgcaa
     2221 atagccgcgc agccg
//
LOCUS       DATANS010000356         3008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_396940, whole genome shotgun sequence.
ACCESSION   DATANS010000356 DATANS010000000
VERSION     DATANS010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396940"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VHT22_05140"
     CDS             complement(<1..664)
                     /locus_tag="VHT22_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3136971.1"
                     /translation="MRFDRAAWWFVAPALLVIAVFFFLPIVAALLISLTDFDIYALAD
                     LANLRFVGFGNYLKLLQTPSFWQALGNTLYFVAAGVPLSIGASLGAALLLHSRLTRFK
                     PFFRTALFAPVVTTLVAVAVIWRYLLNTRYGLLNYALGGLGIDPIDWLGDPHWAMPAI
                     ILFAVWKNFGYNMIIFLAGLQSIPEELYEAARIDGASVWRQFASVTLPMLSPVVMLVG
                     ILT"
     gene            complement(661..1962)
                     /locus_tag="VHT22_05145"
     CDS             complement(661..1962)
                     /locus_tag="VHT22_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX3136972.1"
                     /translation="MNCPGTRARSRAAVLFFAFAAISLASSCARSPPAGETVLKFWAM
                     GREGEVVVQLLPEFERTHPGIRVDVQQLPWTAAHEKLLTAFAGDATPDICQLGNTWIP
                     EFVALGAIAQLDRYVADSPVVDPADYFSGIWQTNIVGGALYGVPWYVDTRLLFYRRDL
                     LGQAGYASPPTSWPEWTRTMVAVKQRAGPDRYAILLPLNEFEPLLALALQQDEPLLRD
                     DGRWGNFDSPGFRRALQFYVDAFRAGFAPASTGIDVSNVWNEFGRGYFAFYISGPWNI
                     AEFKRRLPASVQGEWATAPLPGPNAPGASIAGGSSLVLFRASRSKAAAWQLIEYLARP
                     DVQRRFHELTGDLPPRREPWRDPPLADDVYARAFRDQLERVKPTPQVAEWERIANQMR
                     LVAERVVSGQLSVDEAAKELDARANDILEKRRWMLARGEPH"
     gene            complement(1959..>3008)
                     /locus_tag="VHT22_05150"
     CDS             complement(1959..>3008)
                     /locus_tag="VHT22_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX3136973.1"
                     /translation="HFLDMKTGERFADSELSTVDTAILLAGALFCQSYFDGSDAEEVE
                     IRSLVDSIYRRVDWTWAQPRAPAISLGWSPEEGFLEYDWRGYNEAMLVYLLALGSPTH
                     PVGPEAWAAWTSTYDQSWGKQFGQEYLGFAPLFGHQYTHVWLDFRDVQDAYMRRRGLD
                     YFQNTRRAIYAQQAYAVANPRQCKDYGAMIWGLTASDGPADMELEVAGVMRLYRSYAA
                     RGIGGMGAGTHDDCTLAPTAAVASLPFAPELAIPAVLEMNKRFGNYIYAKYGFLDAFN
                     LNFNFDGPLAHGRCIPGFGWVDGDYLGIDQGAIFTMIENYRSDMIWRVMHTNPYVRTG
                     LQRAGFSGGWLTASR"
BASE COUNT          590 a          981 c          979 g          458 t
ORIGIN      
        1 tcgtcaggat gccgaccagc atgacgaccg gcgagagcat cggcagcgtg accgaagcga
       61 actgtcgcca caccgaggcg ccgtcgatgc gcgccgcctc gtagagctcc tccggaatgc
      121 tctgcagccc cgcgagaaag atgatcatgt tgtagccgaa attcttccat acggcaaaaa
      181 gaatgatcgc cggcatcgcc caatgcggat cgccgagcca atcgatcgga tcgatgccga
      241 gcccgcccag cgcatagttg agcaaaccat aacgggtgtt taacagatag cgccagatga
      301 cggcgacagc taccagcgtc gtcaccaccg gcgcgaacag cgcagtgcga aaaaacggct
      361 tgaatcgcgt cagccgcgaa tgcagcagca gcgcggcgcc gagcgacgcg ccgatcgaca
      421 agggcacgcc ggcggcgacg aagtacagcg tgttgcccag cgcctgccag aacgacgggg
      481 tctgcagtag cttcagatag ttgccaaagc cgacgaaacg cagattggcc agatcggcga
      541 gagcgtagat gtcgaaatcg gtcaggctga tcagaagcgc ggcaacgatc ggcaggaaaa
      601 agaatacggc gatgaccagc aaagccggcg cgacaaacca ccaggcggcg cgatcgaatc
      661 tcaatgcggt tccccgcgcg cgagcatcca gcgccgcttt tcgagaatat cgttggcgcg
      721 cgcgtcgagc tccttcgccg cctcgtcgac cgacagctgg ccggacacca cgcgctcggc
      781 caccagtctc atctggttgg cgatgcgctc ccactcggcg acctggggcg tgggcttcac
      841 gcgctccagc tggtcgcgaa acgcacgcgc atagacgtcg tcggcgaggg gcgggtcgcg
      901 ccacggttcg cgccgcggcg gcagatcgcc ggtcagctca tggaaccggc gctgaacgtc
      961 ggggcgggca aggtattcga tcaactgcca cgccgcagcc ttcgaccgcg aggcgcggaa
     1021 caacaccagg ctcgacccgc ccgcgatcga cgcgcccgga gcattcgggc cgggcaaggg
     1081 cgccgtcgcc cattcgcctt gcacgctcgc cggcaggcgt cgcttgaact cggcgatatt
     1141 ccacggaccg gagatgtaaa acgcgaaata tccgcgcccg aactcgttcc atacgttgga
     1201 gacatcgatg ccggtcgagg ccggtgcgaa gccggcacgg aatgcatcga catagaattg
     1261 caatgcgcgg cggaatccgg ggctgtcgaa attgccccaa cggccatcgt cgcgcagcag
     1321 cggctcgtcc tgctgcagcg ccagcgccag cagcggctcg aattcgttca gcggcagcag
     1381 gatcgcgtaa cgatcggggc cggcacgctg cttgacggcg accatcgtcc gcgtccattc
     1441 cggccacgac gttggcggcg acgcataacc ggcctggccg agcaggtcgc gccggtagaa
     1501 caagagccgc gtatcgacat accagggcac gccatacagc gcacccccga cgatattggt
     1561 ctgccagatg ccgctgaaat agtcggcggg atcgaccacc ggcgaatcgg cgacgtaacg
     1621 gtccagctgc gcgatggcgc ccagcgccac gaattccgga atccaggtat taccgagctg
     1681 gcagatgtcc ggcgttgcat cgccggcaaa ggcggtcagc agcttttcat gcgccgcggt
     1741 ccacggcagc tgctggacgt cgacgcggat gcccggatgc gtgcgctcga actcgggcag
     1801 cagctggacg accacttcgc cctcgcggcc catcgcccag aacttcaata cggtttcgcc
     1861 ggcggggggc gagcgggcgc aggacgacgc cagcgatatg gcggcgaagg cgaaaaagag
     1921 caccgccgcg cgcgagcgcg cgcgcgtgcc cgggcaattc atcgcgacgc ggtgagccag
     1981 ccgccggaga agccggcacg ttgcaagccg gtccggacgt aaggattggt gtgcatcacc
     2041 cgccagatca tgtcgctgcg gtaattctcg atcatcgtga agatcgcccc ctggtcgatg
     2101 ccgaggtagt cgccatcgac ccagccgaat ccgggaatgc agcggccatg cgccagaggt
     2161 ccgtcgaaat tgaagttcag gttgaacgcg tcgagaaagc cgtacttcgc gtagatatag
     2221 ttaccgaagc gcttgttcat ttcgagcacg gcggggatcg caagttccgg cgcgaagggc
     2281 agcgaggcca ccgccgcagt cggcgccagc gtgcagtcgt cgtgggtgcc cgcgcccatg
     2341 ccgccaattc cgcgtgccgc gtagctgcgg taaagccgca tgactcccgc gacttcgagc
     2401 tccatgtccg ccggcccatc gctcgccgtc aggccccaga tcatcgcgcc atagtccttg
     2461 cattgccgcg gattggcgac tgcgtatgcc tgttgggcgt aaatcgcacg ccgcgtgttc
     2521 tggaagtagt cgaggccgcg ccgccgcata taggcatcct ggacgtcgcg gaagtcgagc
     2581 cacacatgcg tgtactggtg gccgaacagc ggggcgaagc ccaggtattc ctggccgaac
     2641 tgcttgcccc aggactggtc gtaggtgctg gtccatgccg cccacgcctc aggcccgacc
     2701 gggtgcgtcg gcgagccgag cgccagcagg tagaccagca tcgcttcgtt gtagccgcgc
     2761 cagtcgtatt cgaggaaccc ctcttcgggc gaccagccga ggctgatcgc cggcgccctc
     2821 ggctgcgccc atgtccagtc gacgcgccgg tagatgctgt cgaccagcga gcggatttcg
     2881 acttcctccg cgtccgatcc gtcgaagtac gattggcaga acaaggcgcc ggccagcagg
     2941 atcgcggtgt cgacggtgga aagctcgctg tcggcgaagc gctccccggt cttcatgtcc
     3001 aggaaatg
//
LOCUS       DATANS010000357         2055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_187486, whole genome shotgun sequence.
ACCESSION   DATANS010000357 DATANS010000000
VERSION     DATANS010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187486"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..>2055
                     /locus_tag="VHT22_05155"
     CDS             313..>2055
                     /locus_tag="VHT22_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX3136974.1"
                     /translation="MLLAAALDGHPATAVLARTTAIEPAAPRRACADMGLVDALARVA
                     ALTSDARAAATSDAKNAAMRALLHTAGWQWAGWMIAGLLLLQLSRRAGLAAPGIAAAL
                     IVAAAAAWLGRVPLPLATDHAFVLGRESTSLWSMPAPFVLALAAAGVLLLLSAPWVSR
                     AFATSAQRPSSAFGYPGLVFATGIGWLVLLDLSANGHPSNRYLALYHQGHLWVGMLAF
                     SVVAFARQPIGRALSWTLSMLDGSASRVAARCGAVGGAAMLIALLIVLIGLVGSLLLN
                     VRQLTSELGRLWLIVGAAWFFFLRGTPFTERMSRSGTSIGSLARYVWPLLFVVAVLIG
                     AMLVTHDMGPLLIAGYGAGAFVAASVAMWWYQRRGAAGVAYGVAVAVFVAWIVVTTGA
                     LFRLGSLDDVGAARLENLTAPLASASDQLALVTWFQRASPAAGFGLGSVPWCGFGAAG
                     TCAGVPVQIQSDYTFTALIGEFGWSAAWAFTIGCAVWLHWMARCHAGATRGEPRFVRA
                     GARIRNDEQAFLSWICVTWVVLSMCQLAVTVAGNIAVIPLTGVTFPFVSFGMTSLIAN
                     AAMLGLAINVNATSG"
BASE COUNT          246 a          722 c          710 g          377 t
ORIGIN      
        1 ccgcgagctc tcatcgacgc gcgcgcgctt ctgcggcagg ctctccaggc gccggtcgtc
       61 gcggccgatg cacggctcgc cgatcttcgg caggcgcaac gcgagggtgt aggcgatccc
      121 gatgtgctgc gcgaggcgat cgcttccgca gaatcccaga tccagcatta ctcaacgcgg
      181 ttcgcgatcg cgcccgctgc aggcgccgaa ccgctcgctt tcgcgtgcgc tttcgagggc
      241 gcgcgtgccg cgctcgccca acccgcgccc gaccccagca gcgccggcgc cgccgcgcgt
      301 gcgaacgcgg tgatgttatt ggccgccgcg ctcgacggac accccgcaac cgctgtgctg
      361 gcgaggacaa cggcgatcga gcccgccgcc ccgcgccgtg cctgcgccga catgggattg
      421 gtcgacgcgc tggcgcgagt cgccgcactg accagcgatg cgcgcgctgc ggcgacgagc
      481 gatgccaaga acgccgccat gcgcgcgctg ctgcacaccg cgggctggca atgggcagga
      541 tggatgatcg ccgggctcct gctgctgcaa ctgagccgcc gtgcgggact tgccgcgccg
      601 ggcatcgcag ctgcgctcat cgttgccgct gccgccgcat ggctcgggcg cgtgcccttg
      661 ccgcttgcga cagatcatgc cttcgtgctg gggcgtgagt cgacgtcgct ttggtcgatg
      721 cccgcgccgt tcgtgcttgc gctcgccgcg gccggggtgc tgctgttgct cagcgcgccg
      781 tgggtctcca gggcgttcgc caccagcgcc cagcggcctt cgtccgcatt cggatacccc
      841 ggtctcgtgt tcgcgacggg catcgggtgg ctcgtcctgc tcgatctgtc ggcgaacggc
      901 cacccgagca atcggtacct cgcgctgtac caccagggtc acctttgggt aggcatgctt
      961 gccttcagcg tcgtcgcctt cgcgcgccag ccgatcggcc gcgcgctctc gtggacgctg
     1021 tcgatgctgg acggcagcgc aagccgcgtc gccgcgcgct gcggcgctgt cggcggcgcc
     1081 gcgatgctca tcgcgctgct catcgtgctg atcggactcg tcggctcgct gctcctcaac
     1141 gttcgccagc tcacttccga gctcgggcgc ctctggctca tcgtcggcgc agcgtggttc
     1201 ttcttcctcc gcggcacacc gttcaccgag cggatgagcc gaagcggcac atcgatcggc
     1261 tcgctcgccc gctacgtatg gccgctcttg ttcgtggtcg cggtgctgat cggcgcgatg
     1321 ctcgtcaccc atgacatggg accgctcctc atcgcgggct acggggcggg cgcgttcgtc
     1381 gcagcatccg tcgcgatgtg gtggtaccag cgccgtggag cggccggcgt ggcgtacggg
     1441 gtcgcggtgg cggtcttcgt cgcgtggatc gtcgtcacga ccggcgcact gttccgattg
     1501 ggctcgctcg acgatgtcgg cgccgcgcgc ctcgaaaatc tcaccgcccc gctcgcgtca
     1561 gcgagcgacc agctcgcgct cgtcacgtgg ttccagcgag cgtcgccggc agccggtttc
     1621 gggctcggct cggttccgtg gtgcggattc ggggcggccg gcacgtgcgc cggcgtcccg
     1681 gtacagatcc agagcgacta cacgttcacg gcgctgatcg gcgaattcgg ctggagtgca
     1741 gcgtgggcat tcacgatcgg ttgcgcggtg tggcttcatt ggatggcgcg atgccatgcc
     1801 ggtgccacgc gcggcgagcc ccgcttcgtt cgcgccgggg cgcgtatccg caacgacgag
     1861 caggcattct tgagctggat ctgcgtgacg tgggtcgttc tcagcatgtg ccagctcgcg
     1921 gtgaccgtcg ccggcaacat cgcagtgatt ccgctcaccg gcgtgacctt tcccttcgta
     1981 agcttcggga tgacttcgct cattgcgaac gcggcgatgc tgggtcttgc gatcaacgtg
     2041 aacgcgacgt cgggc
//
LOCUS       DATANS010000358         1551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_60477, whole genome shotgun sequence.
ACCESSION   DATANS010000358 DATANS010000000
VERSION     DATANS010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60477"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..438)
                     /locus_tag="VHT22_05160"
     CDS             complement(22..438)
                     /locus_tag="VHT22_05160"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEX3136975.1"
                     /translation="MSRLAAVVLTLWAGSLWTICAIAAPAAFAVLERHAAGQLAGTLF
                     QIATWLGAVCALLLFAAAFVHKPLATRLTPAWLVAATAAAPLLSELVLSPMMAAARAA
                     NDMSRFGMLHGLAAVLFVAAALGALLLVLRFNRPAG"
     gene            complement(435..911)
                     /gene="greA"
                     /locus_tag="VHT22_05165"
     CDS             complement(435..911)
                     /gene="greA"
                     /locus_tag="VHT22_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX3136976.1"
                     /translation="MKRMPMTLKGAERLRVELKRLKTEDRPRIIQAIAEARSHGDLSE
                     NAEYHAAREQQSFCEGRIKDIEAHLSNAEIIDVTRLPATGKVVFGATINLEDQDDGNA
                     VVYQIVGDPEADIKSGLISVSSPIARALVGKSQGDVVDVVAPSGTRTYEIVAVKYV"
     gene            complement(908..>1551)
                     /gene="carB"
                     /locus_tag="VHT22_05170"
     CDS             complement(908..>1551)
                     /gene="carB"
                     /locus_tag="VHT22_05170"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095207.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEX3136977.1"
                     /translation="GMTVERVPKFFAVKESVFPFAKFPEADPILGPEMKSTGEVMGTG
                     ASFGEAYAKAQLASGVVLPTRGVCLISVRERDKPAAVTLARRLVAQGFEIVATSGTAK
                     AISDAGIPCRSANKVREGRPHIVDMIKNDEISLIVNTTEGKQAIFESRSIRREAVHKR
                     VTYYTTVAAGLATCDAIAHMNEVDVNRLQDLHQAVSSDTQSLPALALSEGSAS"
BASE COUNT          271 a          515 c          489 g          276 t
ORIGIN      
        1 cgcgcaagga caagccgcgc atcatcctgc cggccgattg aagcgcagca ccagcagcag
       61 cgcaccgagc gcggctgcga cgaacagcac cgccgccagg ccgtgcagca ttccgaagcg
      121 actcatgtca ttggccgcgc gcgccgcagc catcatcggc gagagaacga gctcactgag
      181 aagcggagcc gcagctgttg cagcgaccag ccaggcggga gtgagccgcg tcgcgagcgg
      241 cttgtgcacg aatgcagcgg caaaaagtaa cagcgcgcag actgcaccga gccacgttgc
      301 gatctgaaac agcgtccctg cgagttgccc cgctgcatgc cgttcgagaa cggcgaatgc
      361 cgccggcgcg gcgatagcgc agatcgtcca caggctgcca gcccacagag tcaggacgac
      421 tgccgcaagt ctgctcacac gtatttgacg gcgacgatct cgtacgtgcg cgtgccgctc
      481 ggtgcgacca cgtcgacgac gtcgccctgc gacttgccga ccagcgcgcg cgcgatcggc
      541 gacgacaccg agatcaggcc gcttttgatg tcggcctccg ggtcgccgac gatctggtag
      601 acgaccgcat tgccgtcatc ctgatcttcc aggttgatgg tcgcaccgaa cacgaccttg
      661 ccggtcgccg gcaggcgcgt gacgtcgatg atctccgcgt tcgacaagtg cgcttcgatg
      721 tccttgatgc gcccctcgca gaagctctgc tgctcgcgcg cggcgtgata ctcggcgttc
      781 tcgctgagat cgccatggct gcgcgcctcg gcgatagcct ggatgatgcg cggccggtct
      841 tcggtcttga gccgtttcag ctcgacgcgc aggcgctcgg cacccttcag cgtcataggc
      901 atccgtttca tgacgcgcta ccttcggaaa gcgcaagcgc cggcagggat tgcgtatcgc
      961 tggatacggc ctggtgcagg tcctgcagcc ggttgacgtc cacttcgttc atgtgggcga
     1021 tcgcgtcgca ggtcgcgaga cccgcggcga cggtcgtgta ataagtgact cgcttgtgca
     1081 cggcctcgcg ccggatcgag cgcgattcga agatcgcctg cttgccttcg gtcgtgttca
     1141 cgatgagcga gatctcgtcg ttcttgatca tgtcgacgat gtgcgggcgc ccttcgcgca
     1201 ccttgttggc ggagcggcag ggaatgccgg cgtcgctgat cgccttggcg gttcctgacg
     1261 tcgcaacgat ttcgaagccc tgcgcgacga gacgtcgcgc aagcgttacc gccgcaggtt
     1321 tgtcgcgctc gcggacgctg atcaagcaca caccgcgcgt cggcaacacg acaccggacg
     1381 cgagctgcgc cttcgcgtat gcctcaccga acgatgctcc ggttcccatc acctcacctg
     1441 tcgacttcat ctccgggcca agaatcggat cggcttccgg gaatttcgcg aacgggaaca
     1501 ccgactcctt cacggcgaag aacttcggca cgcgctcgac tgtcatgccg a
//
LOCUS       DATANS010000359         1900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_522878, whole genome shotgun sequence.
ACCESSION   DATANS010000359 DATANS010000000
VERSION     DATANS010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522878"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="VHT22_05175"
     CDS             complement(<1..175)
                     /locus_tag="VHT22_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX3136978.1"
                     /translation="MSLRLGDTAPDFTQESSEGPISFHKWLGDSWGVLFSHPADFTPV
                     CTTELGLTAKLKDE"
     gene            360..1223
                     /gene="serB"
                     /locus_tag="VHT22_05180"
     CDS             360..1223
                     /gene="serB"
                     /locus_tag="VHT22_05180"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEX3136979.1"
                     /translation="MTAESAAAARLVVQAPDIDTPALKALAKLAGAERIDAVSRGGDQ
                     AFALTPAVNAAEVFAYCARESLDCAFIPPGQRLDRVRLLAMDMDSTLITIECVDEIAD
                     MMGIKREVAAITASAMRGEIDFRESLTRRVALLRGLDVAALQRVYDERLDLSPGAERL
                     LAGMRAVGAQSLLVSGGFTFFTERLKARLGLDHTCANTLEIVDGRLTGRLVGPIVDAE
                     AKAACFQRLRATLAADGGLTVAIGDGANDLPMLEAADVSIAYRAKPVVRARATYAIDR
                     CGLDAVLNLFT"
     gene            complement(1232..1645)
                     /locus_tag="VHT22_05185"
     CDS             complement(1232..1645)
                     /locus_tag="VHT22_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX3136980.1"
                     /translation="MNPAPMPLISALYTGVFALLLVALAMLVSRRRRSMGIGLGAGGD
                     AQLERAIRVHANAVEWGLPILLLLLIAEENRASPLLLHICGIVFILARVMHAAGLSRA
                     SGYSVGRFAGIALTWAVLIVLAVWNIVAFLRPALA"
     gene            complement(1642..1839)
                     /gene="iscX"
                     /locus_tag="VHT22_05190"
     CDS             complement(1642..1839)
                     /gene="iscX"
                     /locus_tag="VHT22_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEX3136981.1"
                     /translation="MKWVDSRDIAIALAESHPDVDPATVRFTDLHRWVIALPGFDDDP
                     KHSGEKILEAIQMLWIEETEE"
BASE COUNT          321 a          622 c          640 g          317 t
ORIGIN      
        1 actcgtcctt gagcttggcg gtcaatccga gctcggtcgt gcacaccgga gtgaagtcgg
       61 caggatgcga gaacaacacg ccccagctgt cgccgagcca tttgtgaaag ctgatcgggc
      121 cctcgctcga ttcctgggtg aaatcggggg cggtgtcgcc aagacgcagt gacatggttt
      181 tctcctcgtc ggaccggttg aatcacatat aatacgccgc gctcgcgccg gggagtagaa
      241 gcgatggcac tatcatcata cccgcgccaa ccgcggtggc ggcgggcgca aactccttac
      301 ctccccgatc cgatgcgacg atcccctgac cgcaaatcgc cgtcggcgcg aaggccgcga
      361 tgacggccga aagcgccgcc gcggcgcgcc tggtcgtgca ggcgcccgac atcgacacgc
      421 cggcgctgaa ggcgcttgcg aagctcgccg gcgccgagcg catcgacgcc gtgtcgcgcg
      481 gcggtgatca ggcgttcgcg ctgacgccgg cggtcaatgc cgccgaggtc ttcgcgtact
      541 gcgcgcggga atcgctggat tgtgcgttca tccccccggg gcagcgcctc gaccgcgtgc
      601 gcttgctggc aatggacatg gattcgactt tgatcaccat cgaatgcgtc gacgagatcg
      661 ccgacatgat gggtatcaag cgagaggtcg cggcgatcac ggcgagtgca atgcgcggtg
      721 aaatcgattt ccgcgaaagc ctgacgcgcc gcgtggccct gctgcggggc ctggacgttg
      781 ccgcgctgca gcgtgtctac gacgagcggc tcgatctctc gccaggcgcg gagcgcctgc
      841 tggccggcat gcgcgccgtc ggcgcccagt cgctgctggt ctcgggcggg ttcacttttt
      901 tcaccgagcg tctgaaagcg cggctcggac tcgaccatac atgcgcgaac acgctggaga
      961 tcgtcgatgg caggctgacc ggccgcctgg tcgggccgat cgtcgacgcc gaggcgaagg
     1021 ccgcgtgctt ccagcgcctg cgcgcgacgc ttgccgcgga cggcggcctc acggtggcga
     1081 tcggcgacgg ggcgaacgac ctgccgatgc tggaagccgc cgacgtcagc atcgcctacc
     1141 gcgccaaacc ggtcgtgcgc gcccgcgcga cttatgcgat cgaccgatgc ggactcgacg
     1201 cggtgctcaa tctgttcacg tagcgactgc cttacgccag cgcggggcgc aggaacgcaa
     1261 cgatgttcca caccgcgagc acgatgagca ccgcccaggt cagcgcgatg ccggcgaagc
     1321 ggcccaccga atacccgctg gcgcgcgaca ggcccgccgc gtgcatgacg cgggccagga
     1381 tgaacacgat gccgcagata tgcagcagca gcggcgaggc gcggttctct tcggcgatca
     1441 gcagaagcag caaaatcggg agtccccatt cgaccgcgtt ggcgtggacg cgaatcgcgc
     1501 gctccagctg ggcgtcgccg ccggccccca gaccgatgcc catgctgcgt cgccggcgcg
     1561 acaccagcat tgcaagggcg accagcagca gcgcgaatac gccggtgtag agcgcgctga
     1621 tcaaaggcat cggtgcggga ttcattcttc ggtctcctcg atccacagca tctggatggc
     1681 ctcgagtatc ttttcgcccg agtgcttggg gtcgtcgtcg aagccgggca gcgcgatcac
     1741 ccagcggtgc agatcggtga agcgcacggt cgccggatcg acgtcggggt ggctctcggc
     1801 cagcgcgatc gcgatgtcgc gcgagtcaac ccacttcatt tggtcgtctc tttggcgtag
     1861 ttgatcgtgt atttcggaat ctcgatcacc agcttggtgt
//
LOCUS       DATANS010000360         2813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_313859, whole genome shotgun sequence.
ACCESSION   DATANS010000360 DATANS010000000
VERSION     DATANS010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313859"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VHT22_05195"
     CDS             <1..244
                     /locus_tag="VHT22_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310617.1"
                     /note="catalyzes the decarboxylation of
                     3-octaprenyl-4-hydroxy benzoate to 2-octaprenylphenol;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-octaprenyl-4-hydroxybenzoate decarboxylase"
                     /protein_id="HEX3136982.1"
                     /translation="KEVVWAISTRVDPARDTLIVENTPIDYLDFASPVAGLGAKMGID
                     ATAKWPGETSRQWGRPIAMDAATRRRIDAIWKDLGL"
     gene            complement(292..1263)
                     /locus_tag="VHT22_05200"
     CDS             complement(292..1263)
                     /locus_tag="VHT22_05200"
                     /inference="COORDINATES: protein motif:HMM:NF025082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3136983.1"
                     /translation="MKLWGVAMVRNEADIIEAFVRHNLSVLDGLAIVDHGSTDATPEI
                     LAALSAEGLPLRIEVDAELEFRQSDIITRLTRRILATTDADFIFLLDADEFLKVPSRD
                     RLEAALASLPPGVHAVQEWHTYVPDFSRALDPASLIRSARRVRHGLEPRYRAVVSRDF
                     LQTPTLIAEGNHLVVRRVGAENHPKIQHARLTVDESAVAHIPIRSAAQFGAKIAIGWL
                     SCLMQPGRAPGLSSHWRDSYGLLRSGKALTPETLTLLAANYDVPDSERKPLDQIDFAD
                     DPFFADIEIVHQGAARADALALVVQFAEKLARRVSEADAASGKTRAV"
     gene            complement(1274..1561)
                     /locus_tag="VHT22_05205"
     CDS             complement(1274..1561)
                     /locus_tag="VHT22_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3136984.1"
                     /translation="MSARLDELAARKSLLVAQLRLQRMELALHAGDVRGAVRPAGLIG
                     SAVAQPAAIIALVEAIAPVFGLQRYARWVRLASIAFAVTRIARKWRQSTID"
     gene            complement(1558..1950)
                     /locus_tag="VHT22_05210"
     CDS             complement(1558..1950)
                     /locus_tag="VHT22_05210"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX3136985.1"
                     /translation="MVDPNARPAAGLASALSQLAATALAMVRTRLELATVEFTEERER
                     AKEMLLLLFSTVLVVLFALLFASIFVIAYFWDSYRLTAVGAVTGFYLVLAVIFLVRLK
                     QRMDEKHSPFTATLSQLEEDAEALRRKP"
     gene            complement(1957..2271)
                     /locus_tag="VHT22_05215"
     CDS             complement(1957..2271)
                     /locus_tag="VHT22_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HEX3136986.1"
                     /translation="MTAAKFERSREALVKDFTDVLAEAESLLKQAAKESGEKANDLRG
                     QVEAKLRSAKIRLNDLSDDASERAKAAAKVTDDYVRDNPWQAIGIAAAVGFLAGLALS
                     RR"
     gene            2386..>2813
                     /locus_tag="VHT22_05220"
     CDS             2386..>2813
                     /locus_tag="VHT22_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3136987.1"
                     /translation="MSRLELPALDPTTVTEKRGSGYPEPFKSRMGDRAKRRLGDACGL
                     SKFGVNLVTLAVGAQSALRHWHTLEDEFVYILAGVVTLVTDAGEQTLGPGMCAGYPAG
                     KRDAHHFINRGNVPASYLEIGNRIEGDKCFYPDDDLIWCD"
BASE COUNT          497 a          922 c          931 g          463 t
ORIGIN      
        1 gaaagaagtg gtctgggcga tttcgacgcg tgtcgatccc gcccgcgaca cgctgatcgt
       61 cgagaacacg ccgatcgatt acctggattt cgcctcaccg gtcgccgggc tcggcgccaa
      121 gatgggcatc gacgcgaccg cgaagtggcc cggagaaacg tcgcgccagt ggggacggcc
      181 gatcgcgatg gacgcagcga ccaggcgccg gatcgacgcg atctggaagg acctcggcct
      241 ttaggcgggc gggctcgccg gggaagccgt tcgatcgagg ccggcgggtg atcagacggc
      301 gcgcgtcttt ccggacgcgg cgtcggcctc ggatacgcgg cgcgccagct tctctgcaaa
      361 ctgcacgacc agggcgagcg cgtcggcgcg cgccgcgccc tgatggacta tctctatatc
      421 ggcgaagaac ggatcgtcgg cgaaatcgat ctggtcgagc ggcttgcgct cgctgtcggg
      481 cacatcatag ttggctgcaa gcagggtcag cgtttccggc gtcaatgcct tgcccgaacg
      541 cagaagcccg tacgaatccc gccagtggga ggaaaggccg ggcgcgcgcc ccggttgcat
      601 caggcacgag agccacccga tcgcgatctt ggcgccgaac tgcgcggccg aacgtatcgg
      661 tatgtgcgca actgccgact catcgacagt caggcgggca tgctggatct tcgggtggtt
      721 ctccgcacca acgcgccgga cgaccagatg gttgccttcg gcgatcagcg tcggagtttg
      781 caggaagtcg cgcgacacca ctgcgcggta tcgcggctca agcccgtgcc tgacgcgccg
      841 cgccgagcgg atgagcgatg ccggatccag cgcacgggaa aaatcgggaa cgtacgtgtg
      901 ccactcctgg accgcatgga cccccggcgg caggctcgcc agcgctgctt cgaggcggtc
      961 ccgcgagggg actttgagga attcgtcggc gtcgagcaga aagatgaagt cggcgtcggt
     1021 cgtcgcaagg atgcgacgcg tgagccgggt gatgatgtcc gattgcctga attccagctc
     1081 ggcatcgacc tcgatccgca gtggaagacc ctcggccgac aaggcggcga gaatctccgg
     1141 tgtagcgtcg gtcgagccat gatcgacaat cgccaggccg tcgagcacgc tcagattgtg
     1201 gcggacgaac gcctcgatga tgtccgcctc gttgcggacc atcgcgacgc cccagagctt
     1261 cactgcaggg gcttcagtcg atcgtcgact ggcgccactt gcgcgctatg cgcgtcaccg
     1321 cgaacgcaat cgaggcgagg cgcacccagc gcgcgtagcg ctgcagtccg aacaccggcg
     1381 caatcgcctc gaccagcgcg atgatagccg ccggctgggc gacggcactg ccgatcagcc
     1441 ccgccgggcg caccgcgccg cgcacgtcgc cggcgtgcaa ggcgagctcc atccgctgca
     1501 ggcgcagctg cgccaccagc agagatttcc gtgccgccag ttcgtccagc cgtgcgctca
     1561 tggcttgcgc cgcaaagcct cggcgtcctc ttcgagctgc gacagcgttg cggtgaacgg
     1621 agaatgcttc tcgtccatcc gttgcttgag acggaccagg aagatgaccg caagcacaag
     1681 gtagaagccg gtgaccgcgc cgacggcggt caggcgatag ctgtcccaga aataggcgat
     1741 gacgaaaatc gacgcgaaca gcagcgcgaa cagcacgacc agaaccgtcg agaacagcag
     1801 cagcaacatc tccttcgcac gctcgcgctc ttcggtgaat tccacggtgg cgagctcgag
     1861 ccgcgtgcgc accatcgcga gcgcggtcgc ggccagctgc gacagcgcgc tggccagccc
     1921 tgccgcgggt ctggcgttcg ggtcaaccac tgggtgctag cgtcggctca acgcgagtcc
     1981 ggccagaaag ccgaccgccg ccgcgatgcc gatggcctgc cacggattgt cgcgcacgta
     2041 gtcgtcggtc accttcgcgg ccgccttggc cctttcgctg gcgtcgtcgg acaggtcgtt
     2101 caaccttatc ttggccgatc gcagctttgc ctcgacctgc ccccgcagat cattggcctt
     2161 ctcgcccgat tccttggccg cctgtttcaa cagggattcc gcttcggcca gcacgtcggt
     2221 gaaatccttg acgagcgctt cgcggctgcg ttcgaatttt gctgcagtca tggtcatctc
     2281 caggcaatga gcgcatcggc cgcttggcgc gagcccgata tgccgctcgg cgggtattat
     2341 agcgctgcgc caagtcgcgc cgaaccgctc cggagtgccg tcaagatgag ccgactcgag
     2401 ctgcctgccc ttgatccgac cacggtgacc gagaaacggg gttccggata tccggagccg
     2461 ttcaagagcc gcatgggcga tcgcgccaag cgacgtctcg gcgacgcctg cggcctgtcc
     2521 aagttcggcg tcaacctggt gaccctggct gtcggcgcac aatcggcgct gcgccactgg
     2581 cacacgctgg aggacgagtt cgtctacatc ctcgccggtg tggtgacgct ggtgaccgac
     2641 gccggcgagc agacgctggg gcccggcatg tgcgccggct atccggcggg caagcgcgat
     2701 gcgcaccatt tcatcaaccg cggcaacgtg ccggcaagct atctcgagat cggcaaccgc
     2761 atcgagggcg acaaatgttt ttatccggat gacgatctca tctggtgcga cga
//
LOCUS       DATANS010000361         2715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_397049, whole genome shotgun sequence.
ACCESSION   DATANS010000361 DATANS010000000
VERSION     DATANS010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397049"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="VHT22_05225"
     CDS             complement(<1..922)
                     /locus_tag="VHT22_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX3136988.1"
                     /translation="MTEAIWRWSATRVAAAIRSREISAREAVDASLSRMSSVNGKLNA
                     VTVDLSDAARAAADRADRAVAASDKPLGPLHGVPVTIKENVDQEGCATTNGVVAFRDV
                     IAPADCPAVANLKSAGAIIIGRTNTPAFSFRIDTVNDLRGRTYNPWSKTHTPGGSSGG
                     AASSVAAGITPLAHGTDIAGSIRFPAYACGIAGIRPSFGRVPSYNPTQAAERSLSSQL
                     MSVQGPLARTVADLRVGLAAMAARDPRDPWWVPAPLAGPALPQPMRVAVVTAADDLAG
                     SKLHPHVSAAIANAARWLADAGYEIVDQRTP"
     gene            1017..2516
                     /locus_tag="VHT22_05230"
     CDS             1017..2516
                     /locus_tag="VHT22_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX3136989.1"
                     /translation="MSVIAVGGFQHETNTFAPSKADYAAFEQGGGWPPLTYGADIAPR
                     VADANIPAAGAIAALHALGHKTIGLVWGAASPSAQVTRDAYERIVGDMLARLAAAGPV
                     DGVYLDLHGAMVAEHLDDGEGELLSRVRDLVGQRTPIVASLDLHANVTRAMIENCEGL
                     VAYRTYPHVDMAATGERAARLLDRTLRLGEPAASSFHPLDFLTGLPSQCTLIEPARSL
                     YALLDRLEREHDAVLSFAPGFPMADFPECGMTVFGYGNDAGRVKTAATALAHSVTDAE
                     GDFSMQLFEPDAAVVRAMQRGEPGAPVVLADTQDNPGAGGNGDTTGLLAALLARRAPG
                     AVLGLLIDPASAAQAHAVGVGRKAAFTLGEISGVPGHKPLGGEFSVRALGDGRFTCTG
                     PMFQGFRMDLGPMALLEKDGVRVALASRKCQAADQEMFRHLGVEPKRERILALKSSVH
                     FRADFEPIAREVLVVAAPGPAKADPSMFSWKRLRPGLRLKPLGPAFQPA"
     gene            2536..>2715
                     /locus_tag="VHT22_05235"
     CDS             2536..>2715
                     /locus_tag="VHT22_05235"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX3136990.1"
                     /translation="MPRRFVIALIKHETNTFSPLPTPITSFARRGAISGPAAIAEAAG
                     TNTPLGGFVDLMGEVG"
BASE COUNT          410 a          932 c          949 g          424 t
ORIGIN      
        1 ccggcgtgcg ctggtctacg atttcgtaac cggcatcggc aagccagcgc gctgcgtttg
       61 cgatcgcagc ggaaacatgc ggatgaagct tcgatccggc gaggtcgtcg gcagcggtca
      121 cgacggcgac acgcatcggc tgcggcagcg ccggtccggc cagaggcgcc ggcacccacc
      181 agggatcgcg cggatcgcgc gcggccatcg cggcaagtcc gacgcgcaag tcggcgaccg
      241 tcctcgccag cggtccctgc accgacatca gctgcgacga cagcgagcgc tccgccgcct
      301 gcgtcgggtt gtacgacgga acgcgaccga acgaaggccg gatgccggcg atgccgcagg
      361 cgtaggcggg aaagcggatc gagccggcga tgtcggtgcc gtgcgccagc ggcgtgatgc
      421 cggcggcaac cgaggacgcg gcgccgcccg acgagccgcc cggcgtgtgc gtcttcgacc
      481 acggattgta ggtgcggccc cgcaagtcgt tgacggtgtc gatgcgaaag ctgaaggccg
      541 gcgtattggt ccttccgatg atgatcgcgc ccgcgctctt gagattggcg accgccgggc
      601 agtcggctgg cgcgatcacg tcgcgaaagg caacgacgcc gttggtcgtt gcgcatcctt
      661 cctggtcgac gttttccttg atcgtcaccg gaacgccgtg cagcggtccg agcggcttgt
      721 ccgacgcggc gactgcgcga tcggcgcggt ccgccgcggc gcgggccgca tcggacagat
      781 cgacggtgac cgcattgagc ttgccgttga ccgacgacat gcgcgacagc gacgcgtcga
      841 cagcttcgcg cgccgaaatc tcgcggctgc ggatggccgc cgcgacgcgt gtcgcgctcc
      901 agcgccagat ggcctcagtc atcgacatct cctaaagcgg cgccggggcg gtttgcgcgc
      961 actggcgaat ccggctaatc tcgcccggca gcgggcggcg ctgcccggga ggaacggtga
     1021 gcgtcatcgc ggtaggcggt ttccagcacg agaccaacac atttgcgccg tcgaaggcgg
     1081 actatgccgc attcgagcaa ggcggcggct ggccgccgct gacctatggt gcggatattg
     1141 cgccgcgcgt tgccgatgcc aatatccccg ccgccggcgc gatcgccgcg ctgcacgccc
     1201 tcggccacaa aacgatcgga ctcgtgtggg gcgccgcttc gccatcggcg caagtgacgc
     1261 gcgacgccta cgagcgcatc gtcggcgaca tgctagcacg cctcgctgca gcggggccgg
     1321 tcgacggcgt gtacctcgat ctgcacggcg cgatggtcgc cgagcacctc gacgacggcg
     1381 aaggcgagtt gctctcgcga gtgcgcgacc ttgtaggaca gcgcacaccg atcgtcgcca
     1441 gcctcgacct gcacgccaat gtcacgcgcg cgatgatcga gaattgcgaa ggcctcgtcg
     1501 cttaccgcac ttatccgcac gtcgacatgg ccgcgaccgg agagcgcgca gcgcggctgc
     1561 tcgaccggac gttgcgcctg ggcgagccgg ccgccagctc gtttcacccg ctggatttcc
     1621 tgaccgggct gccttcgcaa tgcacgctga tcgagccggc ccgaagcctg tatgcattgc
     1681 tcgatcgcct cgagcgcgag cacgatgccg tgctgtcgtt tgcaccgggt ttcccgatgg
     1741 ccgatttccc ggaatgcggc atgaccgtgt tcggctacgg aaatgacgcc ggccgcgtca
     1801 agaccgctgc aacggcattg gcccactcgg tcaccgacgc cgagggcgat ttttcgatgc
     1861 agttgttcga gcccgacgcc gcagtcgttc gggcgatgca gcgaggcgag ccgggcgccc
     1921 cggtagtgct ggccgatacg caggacaatc cgggcgccgg cggcaacggc gatacgaccg
     1981 gcttgcttgc cgccctgctt gcgcgccgcg cgccgggcgc ggtgctcggg ctgctgatcg
     2041 acccggcctc ggccgcccag gcccatgccg tcggcgtcgg ccgcaaggcg gcgttcacgc
     2101 tcggcgagat ttccggcgtg ccgggccaca agccgctcgg cggcgaattc tcggtgcggg
     2161 cgctgggcga tggccgattt acctgtaccg ggccgatgtt ccagggcttc cgcatggacc
     2221 tggggccgat ggcgctgctg gaaaaggacg gcgtgcgggt cgcgctggct tcgcgcaagt
     2281 gccaggccgc ggaccaggag atgttccgcc atctcggcgt cgagcccaaa cgagagcgca
     2341 ttctggcgct gaaaagctcg gtccactttc gcgccgactt cgagccgatc gcacgggaag
     2401 tgctggtcgt cgcggcgccc ggccccgcca aggcagatcc ctcaatgttc tcctggaagc
     2461 ggctgcgccc cggtctgcgg ctgaagccgc tcggacctgc gttccagccg gcttgacact
     2521 ggctgccagt ctcgcatgcc gcgccgcttt gtcatcgcgc tgatcaagca cgaaaccaac
     2581 acgttctcgc cgttgccgac gccgatcacc agcttcgccc ggcgcggggc gatcagcggc
     2641 cccgcggcga tcgccgaggc ggccggcacc aacacgccgc tgggcggctt cgtcgacctg
     2701 atgggcgagg tgggg
//
LOCUS       DATANS010000362         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_187616, whole genome shotgun sequence.
ACCESSION   DATANS010000362 DATANS010000000
VERSION     DATANS010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187616"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VHT22_05240"
     CDS             complement(<1..844)
                     /locus_tag="VHT22_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX3136991.1"
                     /translation="MRLRSARRLLILAGSLVAGIAAADGVSSVDDRGVRVALAAPARH
                     IVALAPSMTELVYAAGAGAKLIGVPRFSDYPAAAQAIPQIGDATRIDLERVLSLKPDL
                     IVAWKTGNHAADFERLEKLGFPVFVAEPGTLAAIPRLVRAIASLAGTTEAAAAGAADF
                     ERGIAALGARYRSRSQVRVFYEIWHAPLMTISGQHMISDVVSLCGGINVFAATPVLTP
                     VVSLESVIAARPEIVLGGSSATTPAEFAEPWGHDARYAGLRNVRALYVDPDLIQRQTP
                     RVLDG"
     gene            complement(870..1406)
                     /locus_tag="VHT22_05245"
     CDS             complement(870..1406)
                     /locus_tag="VHT22_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX3136992.1"
                     /translation="MAEIANAGGDREPLAALEDLGRGAAFADQVFSLVGRSQFFAEFN
                     REDVDLLAGYMRVYHAEPGQVIIREGDIGDYMLLMIRGEVDIYKQNQLGEKQLLTSVM
                     PGMTLGEMSMIDGEPRFATCVALKETTFGTLTREDMGTIILEKPSLGAKILIKLVTML
                     SQRLRHTSATLLQYMGSR"
     gene            complement(1442..>2165)
                     /locus_tag="VHT22_05250"
     CDS             complement(1442..>2165)
                     /locus_tag="VHT22_05250"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX3136993.1"
                     /translation="SINEGIRIARELLLELNEMGVPAGCEFLDMITPQYIADLVSWGA
                     IGARTTESQIHRELASGLSCPVGFKNGTDGNIKIAVDAIRAAQAPHHFLSVTKAGHSA
                     IVSTAGNEDCHIILRGGKAPNFDAASVDAAAKGLAEAGLAQRLMIDFSHANSSKNAQK
                     QVEVGADVARQLAAGEDRIFGIMVESHLKAGRQDLVPGKPLVYGQSITDGCISWEDSQ
                     KLLETLADGVRRRRIKLMETEQ"
BASE COUNT          350 a          719 c          698 g          398 t
ORIGIN      
        1 cgccatccaa cacgcgaggt gtctggcgtt ggatcaagtc aggatcgaca tagagcgcgc
       61 gcacgttgcg cagtcccgca taccgcgcgt cgtggcccca tggctcagcg aactccgcgg
      121 gcgtggtggc ggaactgccg ccgagcacga tttcgggccg ggccgcgatc acgctttcca
      181 gcgagacgac cggcgtcagc accggcgttg cagcaaacac attgatgcct ccgcacagcg
      241 agactacgtc gctgatcata tgttgaccgc tgatcgtcat cagcggcgcg tgccatatct
      301 cgtagaacac cctgacctga ctgcgcgagc gatagcgcgc gccgagtgcc gcgatgcccc
      361 gctcgaagtc tgcggcgccc gccgcggcgg cctcggtggt gccggcgagc gaggctatcg
      421 cgcgcaccaa ccgcgggatc gccgccagcg tcccgggctc agcgacgaat acggggaacc
      481 cgagcttttc caggcgctcg aaatcggcgg cgtgattgcc ggtcttccag gcgacgatca
      541 agtcgggctt gaggctcagc acacgctcga gatcgattcg ggtcgcgtcg ccgatttgcg
      601 ggatcgcttg cgcggctgcc ggatagtcgc tgaagcgcgg caccccgatc agcttcgcgc
      661 ctgcgcccgc ggcgtagacc agctcggtca tcgacggggc gagcgctacg atgtggcgcg
      721 ccggggcggc gagcgcgacc cgcacaccgc gatcgtcgac gctgctgaca ccgtcggcgg
      781 cggcgatgcc ggcaacgagg ctgcccgcca gaatgaggag ccgccgcgcg ctgcgtagtc
      841 tcaacggcgg ctgtccagca cggtcactgt taacggctgc ccatatactg cagcagcgtc
      901 gcgctggtgt ggcgcagccg ctgcgacagc atggtgacga gcttgatcag aatcttcgcg
      961 cccagactgg gtttctcgag aatgatcgtg cccatgtctt cgcgggtgag cgttccgaac
     1021 gtggtttctt tgagcgccac gcaggtcgca aagcgcggct cgccgtctat catcgacatt
     1081 tcgccgagcg tcatgccggg catcaccgac gtcagcagct gcttctcgcc cagctggttt
     1141 tgcttgtaga tgtcgacttc gccgcggatc atcagcagca tatagtcgcc gatgtcgccc
     1201 tcgcgaatga tcacctgccc gggttcggca tggtagacgc gcatgtagcc cgcgagcagg
     1261 tcgacgtctt cacggttgaa ttcggcgaaa aactgcgagc ggccgaccag gctgaatacc
     1321 tgatccgcaa atgccgcgcc gcggcccagg tcctcgagcg cggcgagcgg ctcgcgatcg
     1381 cctcccgcat tcgcaatttc cgccatgcat gctccactgc gttacgttgg cgccgttccg
     1441 gctattgctc ggtttccatc agcttgatac gccgccggcg cacgccatcg gcgagcgtct
     1501 cgagcagctt ctggctgtcc tcccagctga tgcagccatc ggtgatgctt tgcccgtaga
     1561 cgagcggctt gcccggcacg agatcctgcc gcccggcttt caggtgactc tcgaccatga
     1621 tgccgaaaat gcggtcttcg ccggcggcga gctggcgcgc gacatcggcg ccgacctcga
     1681 cctgcttctg cgcattcttg ctcgagttcg catggctgaa atcgatcatc aggcgctggg
     1741 cgagaccggc ttcggcgagc cctttggccg ctgcatcgac gctcgccgcg tcgaaattcg
     1801 gcgccttgcc gccgcgcaga atgatatggc agtcctcgtt gcctgcggtc gaaacgatcg
     1861 cggagtgccc cgccttggtc accgacagga agtgatgcgg cgcctgcgcg gcgcggatcg
     1921 cgtcgaccgc aatcttgatg ttgccgtcgg tgccgttctt gaaaccgacc gggcacgaca
     1981 gccctgacgc gagttcgcga tggatctggc tctcggtcgt gcgggcgccg atcgcgcccc
     2041 aggaaaccag gtcggcgatg tactgtggcg tgatcatgtc gagaaactcg cagcctgccg
     2101 gcacgcccat ttcattcagc tcgagcagca gttcgcgcgc aatgcggatg ccctcgttga
     2161 tgctg
//
LOCUS       DATANS010000363         2429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_691721, whole genome shotgun sequence.
ACCESSION   DATANS010000363 DATANS010000000
VERSION     DATANS010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691721"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="VHT22_05255"
     CDS             <1..1335
                     /locus_tag="VHT22_05255"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX3136994.1"
                     /translation="HGSGGADRETADARGRLRVPPDNPSYTLRRVWLSPEEERGYYNG
                     FANEGLWPLCHIAHVRPVFRSADWEQYVRVNRRFADAVRDEAHTEDPVVLVQDYHLAL
                     VPRLIRERLPKATIIMFWHIPWPNPESFSICPWREQLLEGMLGSTILGFHTRFHCQNF
                     VETVDRHLEARIERESSTISYGGQLTRVESYPISIQWPPEWIDSQLSVSLCRAKVLRI
                     ERLPDDVLLGVGVDRMDYTKGILERFLAVERLLEIHPELVGRFVLVQIAAPSRSSLDE
                     YHNFETRVRSLAARINTTFGRDGYEPIRLKVEHHESQSVNEYYRAADVCVVTSLHDGM
                     NLVAKEFVASREDEQGVLVLSQFTGAARELPDALIVNPYHIENCADALYKALTMPADE
                     KRVRMRSMRRLVQEFNVYRWAGAMLLDAARLRQRQRVSARIFAHQRSAGIEA"
     gene            1332..2150
                     /gene="otsB"
                     /locus_tag="VHT22_05260"
     CDS             1332..2150
                     /gene="otsB"
                     /locus_tag="VHT22_05260"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX3136995.1"
                     /translation="MRYALGTASLTQIRSLVSGRPMLAFDIDGTLAPIVAEPWRAHIS
                     EELRACLRSLAARTPVAIITGRAVKDARPLLGFSPHYVVGNHGAEGIPGFEAQSAGFQ
                     RVCAAWLAELSATPDARGWRAMPGIMLEDKQYSLSFHYRHTARPALARELLEQRIARL
                     VPPPDIVHGKRVLNLLPPDAPHKGEALQSLLGLSGCARAMYIGDDVTDEDVFRMRMPE
                     VLSIRVGRKRKSAAEMYLKNQAEVARLVRRLVAMLDGGRQPSRPLGATASPITR"
     gene            2147..>2429
                     /locus_tag="VHT22_05265"
     CDS             2147..>2429
                     /locus_tag="VHT22_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HEX3136996.1"
                     /translation="MSEIAGRVAPSLSLGVIGNCAFNALVDERASIVWCCLPRPDGDP
                     VFHALLGGAPRDDVARGAFDIEIENFARATQHYVTNTAILVTDLFDRQGH"
BASE COUNT          402 a          782 c          817 g          428 t
ORIGIN      
        1 catggcagcg gcggcgcgga ccgcgagacc gccgatgcgc gcgggcggct gcgcgtgcct
       61 cccgacaatc cgagttacac gctgcggcga gtgtggctga gcccggaaga ggagcgcggg
      121 tactacaacg gtttcgccaa cgaagggctg tggccgctgt gccacatcgc ccacgtacgg
      181 ccggtgttcc gttccgccga ctgggagcag tacgtgcggg tcaatcggcg cttcgccgac
      241 gccgtgcgcg acgaggccca caccgaggac ccggtcgtgc tggttcagga ctatcacctg
      301 gcgctggtgc cgcggctgat ccgcgagcgg ctgccgaaag ccacgatcat catgttctgg
      361 cacattccct ggccgaatcc ggaatcgttc agcatctgcc catggcgcga gcagctgctc
      421 gagggcatgc tgggcagcac gatcctcggt tttcacacgc gtttccattg tcagaatttc
      481 gtcgagaccg tcgatcggca cctggaagcc cgcatcgagc gcgagtcgtc gacgatttcg
      541 tacggcggcc agttgacgcg cgtcgagtcg tatccgattt cgatccaatg gccgcccgag
      601 tggatcgatt cgcaattgtc agtctcgctc tgccgcgcca aggtgctgcg aatcgagagg
      661 cttcccgacg atgtcctgct cggcgtcggc gtcgaccgga tggactacac caagggaata
      721 ctcgagcgct ttctcgcggt cgagcgcctg ctcgagatac atcccgagct cgtcggccgg
      781 ttcgttctgg tgcagattgc cgcgccctcg cgctcgtcgc tcgacgaata tcataatttc
      841 gagactcgag tccgctcgct ggcggcgcgc atcaacacga ccttcggccg cgacggctac
      901 gagccgatcc ggctgaaggt cgagcatcac gagtcgcagt cggtcaacga gtactaccgt
      961 gcggccgacg tttgcgtagt caccagcctg cacgacggga tgaacctcgt cgccaaggaa
     1021 ttcgtggctt cgcgcgagga cgagcaaggc gtcctcgtgc tttcgcagtt caccggcgca
     1081 gcgcgcgagc ttccggacgc gctgatcgtc aacccgtatc atatcgagaa ttgcgccgat
     1141 gccctgtaca aggcgctgac gatgccggcc gacgaaaagc gcgtgcgcat gcgcagcatg
     1201 cggcggctgg tgcaggagtt caacgtctac cgctgggccg gtgcgatgct gctcgatgcg
     1261 gcgcggctgc ggcagcgtca gcgcgtatcg gcgcggatat tcgcgcacca gcgcagcgcc
     1321 ggcatcgagg catgagatat gcgctcggga ctgcgtcgct gacgcaaata cgcagtttgg
     1381 tgtcgggccg gccgatgctg gcgtttgaca tcgacggcac gctggcgccg atcgtcgccg
     1441 agccttggcg agcgcatatt tcggaggagc tccgcgcctg ccttcgctcg ctggcggcga
     1501 gaacgccggt cgcgatcatt accggacgcg cggtcaagga cgcgcgcccg ttgctgggct
     1561 tcagtccgca ctatgtcgtc ggcaatcatg gcgcggaagg gatccccggg ttcgaagccc
     1621 aaagcgccgg ctttcagcgc gtttgtgcgg cgtggctcgc cgagctgagc gctacgccgg
     1681 acgcgcgcgg atggcgtgcg atgccgggga tcatgctgga ggacaagcag tattcgctgt
     1741 cgttccacta ccgccatact gcaaggccgg cactggcgcg tgaattgctg gagcagcgta
     1801 tcgcgcgact ggtgccgccg cccgacatcg tgcacggcaa gcgcgtgctc aacctgctgc
     1861 cgccggatgc gccgcacaag ggcgaggcgc tgcaaagcct gctcggcctt tccggctgcg
     1921 cgcgggcaat gtacatcggc gacgacgtca ccgacgagga cgtattccgc atgcgcatgc
     1981 cggaggttct gtcgatccgg gtcgggcgaa agcggaagag cgccgccgaa atgtatttga
     2041 aaaaccaggc cgaggttgcc cggctggtgc gccggctggt cgcgatgctc gatggcggca
     2101 ggcagccgtc ccggccgctc ggtgcaaccg cctccccgat cacgcgatga gcgagatcgc
     2161 cggccgcgtg gcgccaagtc tctcgctggg cgtcatcggg aattgcgcgt tcaatgcgtt
     2221 ggtcgacgag cgcgcctcga ttgtctggtg ctgtctgcca cgaccggacg gcgatccggt
     2281 gttccacgca ctgctcggcg gcgcgccgcg cgacgacgtc gcgcgcggcg cattcgacat
     2341 cgaaatcgag aacttcgccc gcgcgacgca gcactatgtc accaacacgg cgatactggt
     2401 taccgacctg tttgaccggc agggacacg
//
LOCUS       DATANS010000364         4253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_564673, whole genome shotgun sequence.
ACCESSION   DATANS010000364 DATANS010000000
VERSION     DATANS010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564673"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..616)
                     /gene="msrA"
                     /locus_tag="VHT22_05270"
     CDS             complement(59..616)
                     /gene="msrA"
                     /locus_tag="VHT22_05270"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX3136997.1"
                     /translation="MTMTTMPESRQTAVLGGGCFWCLEAVFDQLAGVQSVESGYAGGR
                     TANPTYEAVCGGATGHAEVVRISFDPAVLSFRDLLRVFFSIHDPTTRDRQGNDIGTQY
                     RSVIFCQTPEQKSDAEAVVAELTRDGIWPAPIVTEIAGAATFYPAESYHQEYFERNGR
                     QPYCQAVVAPKVAKFRKHYVDRLKR"
     gene            903..1745
                     /locus_tag="VHT22_05275"
     CDS             903..1745
                     /locus_tag="VHT22_05275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEX3136998.1"
                     /translation="MFAGIRAFALFVVRVLRRLQATQFLRIAGSLSFTTLLGLVPLFT
                     VAFAYVAPYSMFEHWREALEPVLLKFLLPGSSVSARQYLAEFTAKTATLKGIGTALVV
                     LTAVLLIAEVEREINAIWGIYEARSLPRRIIIYALGFVAVPALIGAAVYATSWLIDTS
                     VAAVPLASAALPYVARPFSLAIGTVLLTLTYKLVPARQVPLRAALISGFLAAVALEGA
                     KFGFALYITNVRTYEIVYGALAALPLFLIWIYVCWIILLAGAAVAATLAEHRWGRSKK
                     RRRP"
     gene            1835..2440
                     /locus_tag="VHT22_05280"
     CDS             1835..2440
                     /locus_tag="VHT22_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX3136999.1"
                     /translation="MWSIIQAAGWPIWPLIFASVIALALIFERMWSLRQSVVAPSGMV
                     DKVLSEYKRTGLTPELLTKTAHQGPLGRVLAAGLANVKSPRPVMKEAVEEVGRIVSHE
                     LEQFLTTLGTIAAMSPLLGLFGTVVGMIEIFGSQTAAGSNPILLAHGISVALYNTALG
                     LIVAIPSMIFYRHFRAKVDALILEMEQQAIKLVELAHGERK"
     gene            2517..2936
                     /locus_tag="VHT22_05285"
     CDS             2517..2936
                     /locus_tag="VHT22_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX3137000.1"
                     /translation="MNLRGGRHRDEPEINFIPLIDVLLVILIFLMVTTTYQRVAELKI
                     TLPEANADPMQQRPKEINVGISAQGQYMVDKTLMPFTTVGALAEALSRAAAGAKDPVI
                     IINADANATHQSVIHVMEAARQAGLVHITFATQTTGK"
     gene            2999..4000
                     /gene="lpxK"
                     /locus_tag="VHT22_05290"
     CDS             2999..4000
                     /gene="lpxK"
                     /locus_tag="VHT22_05290"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX3137001.1"
                     /translation="MDLSRHLVAAWFSPSLTPLSVLLAPAALAFRATTIVRRALYRRG
                     LFRIERLPVPVIVIGNITVGGSGKTPLTIALASELAHRGWRPGIVSRGYRATSRAPRA
                     VGVDDDPDVVGDEPLLLARTGLPVWIGADRPAAARALLAAHPDCDLIIADDGLQHYAL
                     ARDIEIAVIDASRGLGNGLPLPAGPLREPAARLGAVDAIVHLGGRGDSKREFAMTLVG
                     ERFLRVNDSAVSADAALFRSGEVHAIAGIGDPARFFAQLAALGIAAHCHPFPDHHRYV
                     AADLALPGATAILMTEKDAVKCKGFADDRCWGLPVRATVEPTLLALVERGLRGCKAA"
     gene            3981..4160
                     /locus_tag="VHT22_05295"
     CDS             3981..4160
                     /locus_tag="VHT22_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX3137002.1"
                     /translation="MDAKLLELLVCPVTKGPLIYDRERQELISKTARLAYPVRDGIPV
                     MLEEEARKLAPAEYE"
BASE COUNT          671 a         1403 c         1409 g          770 t
ORIGIN      
        1 cggtgttctg gtcagtcggc gcagcgctcg ccggcggcgg ctggttcgcg cggcggccct
       61 agcgcttcag gcggtcgacg tagtgcttcc tgaacttggc caccttcggc gcgacgacgg
      121 cctggcaata cggttgtctg ccattgcgct cgaaatactc ctgatggtag ctctcggccg
      181 gatagaaggt cgccgcgccg gcgatctcgg tgacgatggg cgcgggccag attccgtcac
      241 gagtcagctc ggcgaccacc gcctcggcgt cgctcttctg ctcgggcgtc tggcaaaaga
      301 tgaccgaccg atactgcgta ccgatatcgt tgccctggcg gtcgcgggtc gtcggatcgt
      361 gaatcgaaaa gaacacgcgc agcaggtcgc ggaaggacag tacggcggga tcgaagctga
      421 tcctgacgac ttcggcatgc ccggtcgcgc cgccgcagac ggcttcgtag gtcggattgg
      481 cggtgcgccc tccggcgtaa cctgattcca ccgactgtac gccggcgagc tggtcgaaca
      541 ctgcctcgag gcaccagaaa cagccgccgc ccagcaccgc ggtctgtctc gattcaggca
      601 tcgttgtcat ggtcatgacc cttcggatcg tcagtgatca ggatagcgcg attcgcggca
      661 ccctcgcgag tgccttggtc gcgctcgcga ggccgaagct tacgcccggc gacttatctc
      721 gccgcgcgcc gacgaggtgg tgcgataatc acgcgcggga aaacgcaacg tcgaccgcga
      781 aatcacaacc ggatcaccgg tatcgacgac atgactcgat ctgaccccaa tctcgacctc
      841 gtgcgcgata agctccgggg ccatggcgta gtggcgggcg ccgtcggccg gtcggcctag
      901 ccttgttcgc cgggatacgc gccttcgcgt tgttcgttgt gcgggtcctc cggcgcctgc
      961 aggcgacgca gttcttgcgc atcgccggca gcctgtcgtt cacgaccttg ctggggctcg
     1021 tcccgttgtt caccgtggcg ttcgcctatg tcgcgccgta ctcgatgttc gagcactggc
     1081 gggaagcgct ggagcccgtc ctgctcaaat tcctgctgcc cggcagcagc gtgtcggcgc
     1141 gacagtacct ggccgaattc accgccaaga cagcaaccct caagggtatc ggcaccgccc
     1201 tcgtggtgct gacggcggtg ctgctgatcg ctgaagtcga gcgcgaaatc aacgcgattt
     1261 ggggaatcta cgaagcgcgg tcgctgccgc ggcggataat catctatgcg ctgggcttcg
     1321 tcgcggtgcc cgcgctgatc ggcgcggccg tctacgcgac gtcgtggctg atcgacacct
     1381 cggtggccgc cgtgccgctt gcgtcggccg cgctacccta cgtcgcgcgg cctttttcgc
     1441 tggccatcgg caccgtcctg ctgacgctga cctacaagct ggtgccagcg cgacaggtgc
     1501 cgctgcgcgc ggcgttgatc agcggcttcc tcgcagccgt cgcgttggag ggagcgaagt
     1561 tcggcttcgc gctttacatc accaacgtcc gcacctacga gatcgtttat ggcgcgctgg
     1621 ccgcgctgcc gctgttcctg atctggatct acgtttgctg gatcatcctg ctggccggcg
     1681 cggcggtcgc ggcgacactc gccgagcacc gctggggaag gagcaagaag cggcgccgcc
     1741 cgtgatgtcg tcgcgtcgat ggcgcgaacg tcgccttgcc gaatcgcgac gtccaagcta
     1801 gagttcacgc ttcgcgtcga cggagtgccc cctcgtgtgg tcgatcatcc aggcagcagg
     1861 ttggccgatc tggccgctga tatttgcatc ggtgatcgcg ctggcgctga tattcgagcg
     1921 gatgtggtcg ctgcgtcaga gcgtcgttgc gccgtcgggc atggtcgaca aggtgctctc
     1981 cgagtacaag cgcaccggcc tgacgcccga gctgctcacc aagaccgccc accagggtcc
     2041 gctgggccgg gtgctcgctg cggggctggc caacgtcaag agcccgcggc cggtaatgaa
     2101 ggaagcggtc gaggaggtcg gtcgcatcgt cagccacgag ctcgagcaat tcctgacgac
     2161 gctgggtacg atcgcggcga tgtcgccgct gctcggtctg ttcggcacgg tcgtcggcat
     2221 gatcgagatc ttcggttcgc agaccgctgc cggatcgaat ccgatactgc tggcgcacgg
     2281 gatctcggtc gcgctgtaca acaccgcgct aggcctgatc gtcgcgatac cgagcatgat
     2341 cttctaccgt cacttccgcg ccaaggtcga tgcgctgatc ctcgaaatgg agcagcaggc
     2401 catcaagctg gtcgagctcg cgcacgggga gcgcaaataa tggtcgcccc gactgccgca
     2461 tcgagacatg tcggcgcgcc gcggccccgg tcatcgctga cgcacggaag gcggcgatga
     2521 atctgcgcgg cggcagacac cgtgacgagc cggagatcaa tttcattccg ttgatcgacg
     2581 tcctgctggt gatcctgatc ttcctgatgg tgaccacgac ctatcagcgg gtcgcggagc
     2641 tcaagatcac gctgccggaa gccaacgccg acccgatgca gcaacggcct aaggaaatca
     2701 acgtcggcat cagcgcgcag ggacagtaca tggtcgacaa gacgctgatg ccgttcacga
     2761 ccgtcggcgc gctggccgag gcgctaagcc gcgccgcggc cggtgccaag gacccggtga
     2821 tcatcatcaa tgccgatgcc aatgccacgc accagtcggt gatacacgtg atggaggcgg
     2881 cgcgtcaggc cggtctggtc cacatcacgt ttgccaccca gactaccggc aagtagcggg
     2941 ccggttcggg cgcgggccga ccgcgtcagt gtcccgccgt gaatttcagc gaacctcagt
     3001 ggacctcagt cgacatctgg tcgccgcttg gttttcgccg agtctgacgc cgctgtcggt
     3061 cctgctcgct ccggctgcac tcgcgttccg cgccacgacc atcgtccggc gcgcactcta
     3121 tcgtcgtggt ctgttccgga tcgagcgcct gccggtgccg gtcatcgtca tcggcaacat
     3181 caccgtcggc ggtagcggca agacaccgtt gacgattgcg ctggcgagcg agctcgccca
     3241 ccgcggctgg cgcccaggca tagtcagccg cggctaccgg gcgacgagcc gtgcgccgcg
     3301 cgcggtcggc gtcgacgacg atcctgatgt cgtcggcgac gagcctttgc tgctcgcacg
     3361 gaccggcttg ccggtgtgga tcggcgccga ccggcctgcg gcagcgcgcg cgctgctcgc
     3421 ggcccacccc gattgcgatc tgatcatcgc cgacgatggg cttcagcact acgcgctcgc
     3481 ccgcgatatc gaaatcgcgg tgatcgacgc gagccgcggg ctgggcaacg ggctgccgct
     3541 gccggccgga ccgttgcgcg aaccggcagc acgccttggg gcagtcgacg ccatcgtgca
     3601 tctcggcggg aggggtgact ccaagcgcga attcgcgatg acgctcgtcg gcgagcgctt
     3661 ccttcgggtc aacgatagcg cggtctccgc cgatgcggcg ctgttccgct ccggtgaagt
     3721 gcacgccatc gccgggatcg gcgacccggc gcgcttcttc gcgcagctcg ccgccctggg
     3781 catcgctgcg cactgccatc cgtttcccga tcatcatcgc tacgtcgctg ccgacctcgc
     3841 tctgcccggc gccacggcga tcctgatgac cgagaaagat gcggtaaaat gcaaaggatt
     3901 cgccgacgac cgctgctggg gtctccccgt cagggcgacg gtcgagccaa cgctgttggc
     3961 actggtcgag agaggcttgc gtggatgcaa agctgcttga gcttctggtt tgcccggtga
     4021 ccaagggccc gctgatctac gatcgcgagc gccaggaact gatatcgaaa acggcgcggc
     4081 tcgcctatcc ggtccgcgat ggcatcccgg tgatgctcga ggaagaggcg cgcaagctcg
     4141 cgcctgccga gtacgagtga cacgcgacac ggcgccgagg tgacgcgggc ggcgcgtcgc
     4201 gtcctgcgcc gaccggcgtt atgagcggct ggagttcgtc ttgttcaccg tcc
//
LOCUS       DATANS010000365         1821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_18486, whole genome shotgun sequence.
ACCESSION   DATANS010000365 DATANS010000000
VERSION     DATANS010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18486"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1653
                     /locus_tag="VHT22_05300"
     CDS             <1..1653
                     /locus_tag="VHT22_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HEX3137003.1"
                     /translation="KAAAAGSDVGRRLLLLLGPPSGGKSTMAILLKRGLEEYSHTDEG
                     AMYALQGSPMHESPLNLVPASLRAEFRDTYGVEIRGELSPWARDRLEREFEGDFLRMP
                     VERIFLSEATRMGVGTYAPHDPTTADLADLVGSVDLSKVAQYGDEGDPRAWSWSGAVY
                     AASRGMLEMIEILKVKREFLYLLLTLTQEKNVKVSRFPLIYLDETILAHTNLAEFHKF
                     LQEKENEALLDRMVIVKVPYTLSYIEESRIYNKLVSAAPAFRDVHLDPHALRLASVFA
                     VLTRLLKPEREGLDLSKKLRLYAGESVEGFPENEITRLRAESPEEGLTGVSPRFIVNS
                     ISNAITRSDKKSLTSMEVLLALKDSIEFDARVDAKAKKLWIDYLVLARKDFYNRWVKE
                     DVHRALFASFEDEAQQLLDKYLDEVEASLDHREVTDPITSESRPADERFLRSVEDKIK
                     VSESGKMTFRQEVVRKAMVAYKTGEKFTLNSHSRMREAIEQYLFEERRDVLRLVTSAA
                     RPDDEARKKISAVQDRLTAEYGYDEHSAKEALSYVTTLLAQE"
BASE COUNT          349 a          543 c          627 g          302 t
ORIGIN      
        1 aaggcggccg ccgcggggtc cgacgtcggt cgccgcctgt tgctgctgct cggtccgccg
       61 tccggcggca agtcgacgat ggcgatcctg ttgaagcgcg gtctcgagga gtacagccac
      121 accgacgaag gtgcgatgta cgcgctgcaa ggctcgccga tgcacgagtc gccgctcaat
      181 ctcgtgcccg ccagtctgcg cgcggagttc cgcgacacgt atggcgtcga gatccgcggc
      241 gagctcagcc cctgggcgcg cgaccgcctg gagcgcgagt tcgaaggcga cttcctgcgc
      301 atgccggtcg agcgcatctt cctttccgaa gccacacgca tgggcgtggg cacgtatgcg
      361 ccgcacgacc cgacaaccgc ggatcttgcg gacctcgtcg gctccgtgga tctgtcgaag
      421 gtcgcgcagt acggcgacga aggcgatccg cgcgcctggt cgtggtcggg cgcggtgtat
      481 gccgcgagcc gcggcatgct ggagatgatc gagatcctga aggtcaagcg cgagtttctg
      541 tacctgctgc tcacgctgac gcaggagaag aacgtcaagg tgtcgcgctt cccgttgatc
      601 tacctggacg agacgatcct cgcgcacacg aacctcgccg aattccacaa gttcctgcag
      661 gaaaaggaaa acgaagcgct gctcgaccgc atggtcatcg tcaaggtgcc gtacacgctg
      721 tcgtacatcg aagagtcgcg catctacaac aagctggtgt cggcagcgcc ggcgttccgc
      781 gatgtgcatc tcgatccgca cgctctgcgg ctcgcatcgg tgttcgcggt cctcacgcgg
      841 ctgctcaagc cggagcgcga aggcctcgac ctgtcgaaga agctgcgctt gtacgcgggc
      901 gagagcgtgg aaggtttccc ggagaacgag atcacgcgcc tgcgcgcgga gtcgcccgaa
      961 gagggcctga cgggcgtgag cccacgcttc atcgtcaact cgatctcgaa tgcgatcacg
     1021 cgtagcgaca agaagagcct gaccagcatg gaagtgctgc tcgcgctcaa ggattcgatc
     1081 gagttcgacg cccgcgtgga tgcgaaggcg aagaagctgt ggatcgacta cctggtgctg
     1141 gcgcgcaagg acttctacaa ccgctgggtg aaggaagacg tgcatcgcgc gctgttcgcg
     1201 tcgttcgagg acgaagcgca gcagctgctg gacaagtacc tggacgaagt ggaggcctcg
     1261 ctcgaccatc gcgaggtcac cgatccgatc accagcgagt cgcgtccggc ggacgagcgc
     1321 ttcctgcgct cggtcgagga caagatcaag gtgtcggagt cgggcaagat gactttccgc
     1381 caggaggtcg tgcgcaaggc catggtcgcg tacaagaccg gcgagaagtt cacgctcaac
     1441 agtcactcgc gcatgcgcga agccatcgag cagtacttgt tcgaagagcg tcgcgacgtg
     1501 ctgcggctgg tgacctccgc ggcgcgtccg gacgacgagg cgcgtaagaa aatctcggcg
     1561 gtgcaggacc gcctcactgc ggagtatggt tatgacgagc acagcgcgaa ggaagcgctg
     1621 agctacgtga cgacattgtt ggcgcaggag tagcggcgtt taaagatgag agtgaggttg
     1681 cgaaggggca catgagcgaa aacacgtcgg gcggaggtgg ctccggcggt ggtggcggca
     1741 gcccgggcga ggcgggctgg tacgatctgt tctcgcgcgg tgcacgcgac tggctgcgtc
     1801 acaacgacaa agtccgcgac g
//
LOCUS       DATANS010000366         3571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_440198, whole genome shotgun sequence.
ACCESSION   DATANS010000366 DATANS010000000
VERSION     DATANS010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440198"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /locus_tag="VHT22_05305"
     CDS             complement(<1..1505)
                     /locus_tag="VHT22_05305"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3137004.1"
                     /translation="MADISTLGKYEIRHEIGRGAMGVVYEGYDPMIKRIVALKTIRAD
                     QLAGAEGPNVIARFRREAQAAGRLNHPNIVSIYEFGEDAGTWFIAMEKVEGRELKQCF
                     ETNERFTTADIVRIMSQVLDALDYSHRRGVVHRDIKPANIFLLPGGVVKVGDFGIAHI
                     ETSNLTQVGAVMGTPSYMSPEQIMGLPVDGRSDLFSAGVILYQFLTGERPFAGSATTT
                     MQKVLKEDPLPPSSLNVQAPAGMDAVVRKALAKRPEDRFQTAHEFADALRAAAPGVPV
                     AAADPTLVGAADATVLAAPTSAATTGRAAAAVPPEKKSQTAAVAVAIGVAAVAIGAVA
                     WYLAQRPSAAPAATTQTQSAPTASQPAPAVPAPAPAAAAPVAATAPGTMLITAVGLID
                     PSDPRYRADKALLQSDLRADSKSQLVEKALGLLLDSNSLARNYDVLKAGLLSQSASFV
                     PAIIKEGEPQLGKDGLMSITTQGVVNVRAVQKSLNQMSRDERIDFIRASGDP"
     gene            complement(1580..2398)
                     /locus_tag="VHT22_05310"
     CDS             complement(1580..2398)
                     /locus_tag="VHT22_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137005.1"
                     /translation="MSNRKSVRFSVVALGATLMAVASLALAQPRFGNPSPTAGSDTSQ
                     RANSSADQAMYAPVEYTNVRKKGPAVIVIPGEMKSNNATFLQKFTANNIADFGEIELA
                     SANFPVLERSNLGPIQREFELAYNLGDPDAARKFLRMGKLKSTKYIVKFDILKTEQVA
                     AAQQGFDGHAIGQMAGLLGGFGRGASAASVGVGSVQTSESSGVWIIGMRYKIINAETT
                     EQVATGYTEEKMEVGAKSTSVLGVSQAQQGGVSLDTMVQRLVQKSVWEIDAKYK"
     gene            complement(2446..3135)
                     /locus_tag="VHT22_05315"
     CDS             complement(2446..3135)
                     /locus_tag="VHT22_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137006.1"
                     /translation="MRPKPVFAIFFMLAVLAAGPAAAAEGFKFSEQDEADRAESEARQ
                     SRIAEQLSTPCRADLKGKKIMVVIGEMRSNGVIYAQQQNYGPHFQAINTRLRGLGLRS
                     YTPEEIRRQIAQAEIDAYFRNDPDAALAASKRLGASFVLRGLISSQAQPNPMMNVNQV
                     SVNMDFNLAGSDGRQISTAQAHSSSYAGADVGGMALTLVSENADEVVAKLYADYCTRA
                     GLGGGRKPASK"
     gene            complement(3303..>3571)
                     /locus_tag="VHT22_05320"
     CDS             complement(3303..>3571)
                     /locus_tag="VHT22_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX3137007.1"
                     /translation="QVFEDRVSRALGKLGVHTSADVERLSARVNELSEAVNALLKAQG
                     IKRKAPGKGGRPAAKTASDKAGTAAKTAPAATGTAKKAGKSAEK"
BASE COUNT          548 a         1212 c         1125 g          686 t
ORIGIN      
        1 gggtcgccgc tggcccggat aaagtcgatg cgctcgtcgc gcgacatctg attaagcgat
       61 ttctgcaccg ccctgacgtt gaccacgcct tgcgtcgtga tcgacatcaa gccgtccttg
      121 cccagctgcg gctcgccttc cttgatgatc gcgggcacga agctggcgct ttgcgacaga
      181 agcccagctt tcagcacgtc gtagtttcgc gccagcgagt tcgagtcgag cagcagcccc
      241 agcgcttttt ccaccagctg gctcttggag tcggcgcgca aatcgctttg cagcagcgcc
      301 ttgtcggcgc ggtagcgcgg atcgctggga tcgatcagcc cgacggcggt gatcagcatc
      361 gtgcccggtg cggtcgcggc gacgggtgct gccgcggccg gcgcaggcgc gggcacggcg
      421 ggtgccggtt gcgaggccgt cggcgccgat tgcgtctggg tcgtcgccgc cggtgcggcc
      481 gacggccgct gcgccaagta ccacgcgacc gcaccgatcg ccaccgcggc tacgccgatc
      541 gcgacggcga ccgcagccgt ctgcgatttc ttctcaggcg gaaccgccgc cgcggcgcgg
      601 cccgtggttg cggcagacgt cggcgcagcg agcactgtcg catccgcggc gccgaccagc
      661 gtgggatcgg cggccgccac cggcacgccg ggcgcagccg cgcgcaacgc gtcggcgaat
      721 tcgtgagcgg tctgaaagcg atcctccgga cgcttggcca gcgctttgcg cacgacggca
      781 tccatgccgg caggcgcctg cacgttgagg ctcgacggcg gcagcgggtc ttccttcagt
      841 accttttgca tcgtcgtcgt cgccgacccc gcaaacgggc gctcgccggt caggaactgg
      901 taaaggatga cgccggccga aaacaggtcg gagcgcccgt cgaccggcag tcccatgatc
      961 tgctccggcg acatgtagct gggcgttccc atcaccgcgc cgacctgcgt caggttggac
     1021 gtctcgatgt gggcgatacc gaaatcgccc accttcacca caccgccggg cagcaggaag
     1081 atgttcgccg gcttgatgtc gcggtggacg acgccccgcc ggtgcgagta gtcgagagcg
     1141 tcgagcacct gcgacataat gcgcacgatg tcggccgtcg tgaaacgctc gttcgtctcg
     1201 aagcattgct tcagctcgcg gccctcgacc ttttccatcg cgatgaacca ggtgccggcg
     1261 tcttcgccga attcgtaaat cgacacgatg ttgggatggt tgagcctgcc ggcggcctgc
     1321 gcctcgcggc ggaaccgcgc gatcacgttc gggccttccg cgccggcgag ctgatcggcg
     1381 cgtatcgtct tcagcgcgac gatgcgcttg atcatggggt cgtagccttc gtagacgacg
     1441 cccatcgcgc cgcggccgat ctcgtgccgg atctcgtact tgcccagcgt gctgatgtcc
     1501 gccatgcctg gccccccgtt gcccgaaaac ggcgacgccg gcagcgcgcc ggcgtcgctt
     1561 gccgtttagc gccagccgtc tacttgtact tggcgtcgat ctcccagacc gacttctgca
     1621 ccagccgctg caccatcgta tccagcgaca cgccgccctg ctgggcttgg gagacgccaa
     1681 ggaccgaggt cgacttcgct ccgacttcca ttttttcctc ggtgtagccg gtcgccacct
     1741 gctcggtcgt ttcggcattg atgatcttgt agcgcatgcc gataatccac acgccgctcg
     1801 actcgctggt ctgcaccgag ccgacaccga cgctcgcggc cgaggcaccg cgcccgaaac
     1861 caccgagcag ccctgccatc tggccgatcg cgtggccatc gaagccctgc tgggccgcgg
     1921 cgacctgctc ggttttgagg atgtcgaatt tcactatgta cttggtgctc ttcagcttgc
     1981 ccatgcgcag gaacttgcgc gccgcgtcgg gatcgcccaa gttgtaagcg agctcgaact
     2041 cgcgctggat cgggccgaga ttggagcgct cgagcacggg gaagttggca gacgccagct
     2101 cgatctcgcc gaagtccgcg atgttgttcg cggtgaattt ctgcagaaag gtcgcgttat
     2161 tgcttttcat ctcgcccggg atcacgatca ccgcggggcc tttctttctt acgttcgtgt
     2221 actcgaccgg cgcatacatc gcctggtccg ccgaggaatt ggctcgctgc gacgtatcgc
     2281 ttcctgcagt cggcgaaggg ttgccgaatc tgggctgggc gagcgccagc gacgcgacgg
     2341 ccatcagcgt cgcgccgagc gcgaccacgg aaaagcggac ggacttgcga ttgctcatgg
     2401 caattgctcc tgtcaaattc ctgtggataa cgagctaaag catcctcatt tcgacgcggg
     2461 tttcctgccg ccgcccagtc cggcgcgagt gcagtaatcc gcgtacagct tggccactac
     2521 ctcgtctgca ttctcgctga cgagggtcag cgccatgccc cccacgtcgg ccccggcata
     2581 ggagctcgaa tgcgcctgcg cagttgatat ctgcctgccg tcgctgccgg ccaggttgaa
     2641 atccatgttc accgaaacct ggttgacgtt catcatcggg ttgggctggg cttgcgacga
     2701 gatcaacccg cgcagaacga aactcgcccc gagtcttttc gatgccgcga gcgccgcgtc
     2761 gggatcgttg cgaaagtacg catcgatctc ggcctgcgcg atctggcgcc gaatctcctc
     2821 cggcgtgtag ctgcgaagcc cgagcccgcg caagcgcgtg ttgatcgcct ggaaatgcgg
     2881 gccgtaattc tgctgctgcg cgtagatcac gccgttggag cgcatctcgc cgatgaccac
     2941 catgatcttc ttgcccttga gatcggcgcg acaaggtgtc gacagctgct cggcgattcg
     3001 gctctgccgc gcttcgcttt cggccctgtc ggcttcgtcc tgctccgaaa acttgaatcc
     3061 ctcggcagca gcggccggcc ccgccgccag cacggccagc atgaagaaga tcgcgaagac
     3121 gggcttcggt cgcatcgggg ctcgcatgaa tgcgcaggaa acgccgcgcg gatgatcgcg
     3181 tacggcaacc gtggaaggct agcgcaacgg tggcgggaaa tccacaccga atattcggta
     3241 tttgccgccg cgccaccgct ctgtgggcga cagcaagccg cctgcttcgg cgcggctttg
     3301 tcctactttt cggcggactt ccccgctttt ttcgccgttc ccgtcgccgc gggcgcggtc
     3361 ttggccgcgg tgcccgcctt gtccgacgcg gtcttcgcag ccgggcgccc acccttgccc
     3421 ggcgccttcc gctttatgcc ttgcgctttc agcagcgcgt tgaccgcttc ggacaattcg
     3481 ttgacgcgcg cgctcaggcg ttcgacgtcg gccgacgtat ggacacccag cttgcccagc
     3541 gctcgcgaca ctcgatcctc gaacacctgc t
//
LOCUS       DATANS010000367         2925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_480726, whole genome shotgun sequence.
ACCESSION   DATANS010000367 DATANS010000000
VERSION     DATANS010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480726"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /locus_tag="VHT22_05325"
     CDS             <1..1520
                     /locus_tag="VHT22_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX3137008.1"
                     /translation="AALPAAASARAYASLAQSFDPVDGGFGQAPKFPHAAELDFCLRR
                     YAATRDEHALRMVRVTLARMAEGGIHDQLAGGFSRYSVDGEWTIPHFEKMLYDNASLL
                     ALYADAWRATGEPAFEKTARGIVAWLQAEMRAPDGGFYSSLDADSEHEEGKFYVWTPD
                     QVKAELDPVEYAVAARHWGLDRAPNFEHAAWHLRVTAPLEQVARELSLPLSEVEASLE
                     AARAKLLAARARRVRPGRDDKLLCSWNALMIAALARASRVFGEPSWLSDAQQATDFIR
                     RSMWLDKRLLATYKDGRAHLNAYLDDYAFLLAALIELLQTAFREEDLSWATEIAEALL
                     TRFEDRAGGGFFFTSDDHETLLLRPKPGHDNATPAGNGIAAQALIALGHWLSEPRYLD
                     SAERTVRAFGHELAARPAGFSSLLVALESCLEPPAMLLLRGEPAGCLSWQRGVERQYR
                     PSLQTLNLSQLQGLPGALAKPHESGAGPVTAWLCRGTVCLPPLHALSDLDAVLAS"
     gene            1597..1923
                     /locus_tag="VHT22_05330"
     CDS             1597..1923
                     /locus_tag="VHT22_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX3137009.1"
                     /translation="MKRHGIALAVILFAAGNAHAALDNAAAQALMQKDGCAACHAIDK
                     KVVGPAYQDVAAKYKGDKDALAKLSNKVKRGGSGVWGSIPMPPNTQVSDADIKNLVDW
                     ILTLKK"
     regulatory      1991..2086
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2245..>2925
                     /locus_tag="VHT22_05335"
     CDS             2245..>2925
                     /locus_tag="VHT22_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HEX3137010.1"
                     /translation="MLKQTPLHAIHRAAGARMVDFGGWDMPVHYGSQIDEHHAVRRGA
                     GMFDVSHMLAVDIEPREPAASQSGGRDFLRGALANNVDKLTAPGKALYSCLLRPDGGV
                     LDDLIVYFLREDWFRLVVNAGTADKDLAWLGALIAERGPHLKLTPRRDLAMIAVQGPH
                     ARDRVWSVLPAARAGSESLKPFNAAQVGEYLIARTGYTGEDGFEIALPAALAPDLWQA
                     LQAAGVKAC"
BASE COUNT          487 a          972 c          982 g          484 t
ORIGIN      
        1 ccgcggcgct gccggcggca gcatccgcga gagcctatgc atcgctcgcg caatcgttcg
       61 accccgtcga tggcggtttt ggccaggcgc cgaaattccc gcacgcggcc gagctggact
      121 tttgcctgcg ccgttacgcc gcgacacgcg acgagcacgc gctgcgcatg gttcgcgtca
      181 cgctcgcgcg catggcggaa ggcggcatcc acgaccagct ggcaggcgga ttctcgcgct
      241 atagcgtcga cggcgaatgg acgattccgc acttcgaaaa gatgctctac gacaacgcgt
      301 cgctgctcgc gctttacgcc gatgcctggc gcgcgaccgg tgaacccgcc ttcgagaaaa
      361 ccgcacgcgg aatcgtggcg tggctgcaag cagaaatgcg cgcacccgac gggggtttct
      421 attcgagcct cgacgccgac agcgagcacg aggaaggcaa gttctacgtg tggacgcccg
      481 atcaggtgaa ggcggagctc gatccggtcg aatacgcggt cgccgcccgg cactggggcc
      541 tggaccgggc gccgaacttc gagcacgcgg cttggcacct gagggtcacg gcgcccctcg
      601 agcaggttgc ccgcgagctt tcgctgccgt tgtccgaggt cgaggccagt ctcgaggctg
      661 cacgcgccaa gctgctggcg gcgcgcgcaa gacgcgttcg cccgggccgc gacgacaagc
      721 tcctctgctc gtggaacgcg ctaatgatcg cagcgctggc gcgtgcctcc cgcgtgttcg
      781 gcgagccttc gtggctgtcg gacgcacagc aggcgacgga cttcatccgg cggtcgatgt
      841 ggctcgataa gcggctgctc gcgacgtaca aggatgggcg cgcgcacttg aatgcttatc
      901 tcgacgacta cgcctttttg ctcgcggcct tgatcgagct tctgcagacg gcgtttcgcg
      961 aggaggactt gtcttgggca accgaaattg ccgaagcctt gctcacccgc ttcgaggatc
     1021 gcgccggcgg cggatttttt ttcaccagcg acgaccacga aaccctgctg ctccggccga
     1081 agcccggaca cgacaatgcc acgccggccg gcaacggcat tgccgcgcag gcgctgatcg
     1141 cgctcggcca ctggctgtcg gagccccgct atctggacag cgccgagcga acggtgcgcg
     1201 cgttcgggca cgagctggcc gcacggccgg ctggcttttc atcgctgctg gtagcgctcg
     1261 aatcctgcct cgagccgccg gcgatgctgt tgctgcgcgg cgagcccgcc ggctgcctgt
     1321 cctggcagcg cggcgtcgaa cgtcagtatc gtccgtcgct gcagacgctt aacctgtcgc
     1381 agctgcaggg gctgccgggt gcgctggcga agccccacga gtcaggcgcc gggccggtca
     1441 ctgcttggct ttgccgtggc accgtgtgct tgccgccgct gcacgcgctg tccgacctgg
     1501 acgcagttct cgcgtcctga cgggctggcg gttagcagcg tcgacggcgt tccggtacca
     1561 tcaccgtcga tctgccacca ctctcggaga caaaaaatga aacgccacgg aatcgcgctc
     1621 gccgtcatct tgtttgcggc cggcaacgcg cacgccgcgc tggacaacgc cgctgcgcag
     1681 gcgctgatgc aaaaagacgg ttgcgccgcc tgccatgcga tcgacaagaa ggtcgtcggt
     1741 cccgcgtatc aggacgtcgc cgccaaatac aagggcgaca aggatgcgtt ggccaagctg
     1801 agcaacaagg tgaaaagagg cggtagtggc gtctggggtt cgattccgat gccgccgaac
     1861 actcaggtca gcgacgccga catcaagaat ctggtcgact ggattctgac gctgaagaag
     1921 taagcgccga aacccgtcgc cgtcggctgc gggcgttttt gacagccgtc gacgcggccg
     1981 gtaagcttgc ccggctcgtg caggagaggg cagccgcacc ttggaccggc tgccgccgaa
     2041 ggcgtaactc ccgcgatcgc tcaggcaaaa ggactgcgcg agccgtaagt cactctggag
     2101 agcggccgcg cgtttgatgc agcggtccac cgaaggggca ccggctcaag ccgaagggcg
     2161 agggccgcaa tctctcaggt aaaaaggaca gacggggcga agccgacatc acggcctcgc
     2221 cctttttgct gcctgggagg cgtgttgctc aagcaaaccc cgctccatgc cattcatcgc
     2281 gctgccggcg cccggatggt cgatttcggc ggttgggaca tgccggtcca ctacggctcg
     2341 cagatcgacg agcatcacgc ggtgcgccgc ggcgcgggaa tgttcgatgt ctcgcacatg
     2401 cttgcggtcg acatcgagcc gcgcgagccg gccgcgagcc aaagcggcgg gcgcgacttc
     2461 ctgcgcgggg cactcgccaa taatgtcgac aagctgaccg cgccgggcaa ggcgctttac
     2521 tcctgcctac tgcgtcccga cggcggcgtg ctcgatgacc tgatcgtcta tttcctccgc
     2581 gaagactggt tccggctggt cgtcaacgcc ggcacggccg acaaggacct cgcctggctc
     2641 ggcgcgctta tcgccgaacg tggcccgcat ctcaagctga ccccgaggcg tgacctggcg
     2701 atgatcgcgg tgcaagggcc gcacgcgcgc gaccgcgtct ggagcgtcct gccggcggcg
     2761 cgcgcgggga gcgaatcgct gaaaccgttc aacgccgcgc aggttggcga gtatttgatc
     2821 gcgcgcaccg gctacaccgg cgaggacggt ttcgagatcg ctctgccggc cgcgctcgcg
     2881 cccgacctct ggcaggcatt gcaggccgcg ggcgtcaagg cctgc
//
LOCUS       DATANS010000368         1691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_691966, whole genome shotgun sequence.
ACCESSION   DATANS010000368 DATANS010000000
VERSION     DATANS010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691966"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VHT22_05340"
     CDS             <1..447
                     /locus_tag="VHT22_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX3137011.1"
                     /translation="LALRDAGYYTIDALRIEAGRRAWGAELGPDETPLEAGLAYAVRI
                     DKASNFIGRVALLRQQAEGLKKRLLLFTFDDPAAFPWGGEPISMNGQSVGELTSAGYS
                     RKHGRAVAMGYARSDRVLSDAELLAARYQVDIAGDLFAVTPHLKPA"
     gene            574..1404
                     /locus_tag="VHT22_05345"
     CDS             574..1404
                     /locus_tag="VHT22_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX3137012.1"
                     /translation="MALRSIAPRRGRTPHKPGTPTAQVQSLTRGLSILEALAQADSGL
                     SLTEVGHRVQLPVSTTHRLLATLEKMGYVYQAGDLGLWHIGLQAFTVGSTFLANRDFI
                     GSSHAYMRRLMEQSGETANLAILDGTEAVFIAQVQCHETMRTLVKLGSRVPLHASGVG
                     KTIFAALPDEQIDAILKVRGLPRITENTIVAPETMWAALRVIRQRGYSFDDEEHARST
                     RCVAATVYDEHAEPLGAISLAGPSSRLSDERIRQLGPLVAHTAEELTRTLGGKWPHPY
                     "
     gene            complement(1431..>1691)
                     /locus_tag="VHT22_05350"
     CDS             complement(1431..>1691)
                     /locus_tag="VHT22_05350"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX3137013.1"
                     /translation="KGRDPQSAAVVAELAKVREAGAAIRALGMRFNAGHALNYFNVQS
                     IAAMPGVRELHIGHAIVSRAVFVGLREAVRQMKALLREAAAG"
BASE COUNT          291 a          578 c          558 g          264 t
ORIGIN      
        1 ctggctttgc gcgatgccgg ctattacacg atcgacgctt tgcgcatcga agccggacgg
       61 cgggcctggg gcgccgaact cggccccgac gagacccctc tcgaagccgg gctcgcgtac
      121 gcggtcagaa tcgacaaagc atcgaatttc atcggccgcg ttgcgctgct gcgacagcag
      181 gcggaaggcc tgaagaaaag gctgctgctg ttcaccttcg acgacccggc cgcctttccc
      241 tggggcggcg aaccgatttc gatgaatggg caaagcgtcg gtgaattgac ctcggccggc
      301 tacagccgaa agcacggccg cgcggtggcg atgggctatg cgcgcagcga ccgggtgctg
      361 agcgatgccg agcttctcgc cgcacgttac caggtcgaca tcgccgggga tctgttcgcc
      421 gtcacgccgc acctgaagcc ggcgtgagac caagctgacc cgacctgagc gaaactgaga
      481 cccaaccgaa ccaagctgag gccgctctgc cgagcggcgg ccgcggcaat gcgttattgt
      541 tgcattccga atagcgacga ctgctgactc ccgatggctc tccgctcgat cgcgccgcgg
      601 cgcggccgca cgccgcacaa gccggggacg ccgaccgcgc aggtgcaaag cctgacgcgc
      661 ggattgtcga tcctcgaggc tctggcccag gccgacagcg gcctctcgct gaccgaggtg
      721 ggccatcgcg tgcagttgcc ggtgtcgacg acgcaccggc tgctcgcaac cctggagaaa
      781 atgggctacg tctaccaggc cggcgacctc ggcctgtggc acatcggcct gcaagcgttc
      841 accgtcggca gtacgttcct ggccaaccgc gactttatcg gcagcagtca cgcctacatg
      901 cgccggctga tggagcagtc gggtgaaacc gcgaacctgg cgatactcga cggcaccgag
      961 gcagtattca tcgcgcaggt gcaatgccac gagacgatgc gcacgctggt caagcttggc
     1021 agccgcgtgc ctctgcacgc gtcgggcgtc ggcaagacga tttttgccgc gctgcctgac
     1081 gagcagatcg acgcgatact caaggtgcga ggcttgccgc gcatcaccga aaacacgatt
     1141 gtcgcgcccg aaacgatgtg ggcggcgctg cgcgttatcc gccagcgcgg ctattcgttc
     1201 gacgacgagg agcacgcgcg ctcgacccgg tgtgtcgcag cgacggtgta cgacgagcac
     1261 gccgaaccgc tcggcgcgat ctcgctggcg ggcccctcgt cgcgcctgtc cgacgagcgc
     1321 atccggcagc tgggtccgct ggtcgcgcat accgccgagg agctgacccg cacgctcggc
     1381 ggcaagtggc cgcatccgta ctgacctgaa gaggatcgcc cgccgcgcgc ttatccggcc
     1441 gcggcctcgc gaagcagggc tttcatttgc cgcaccgcct cgcgcagccc gacgaagact
     1501 gcgcggctga cgatcgcgtg cccgatgtga agctcgcgca ccccgggcat ggcggcgatc
     1561 gattgcacgt tgaagtagtt gagcgcatgc ccggcattga agcgcatgcc cagagcgcgg
     1621 atcgccgcgc cggcctcgcg gaccttcgcc agctccgcga cgaccgccgc gctttgcgga
     1681 tcgcgaccct t
//
LOCUS       DATANS010000369         4362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_60978, whole genome shotgun sequence.
ACCESSION   DATANS010000369 DATANS010000000
VERSION     DATANS010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60978"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VHT22_05355"
     CDS             complement(<1..349)
                     /locus_tag="VHT22_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX3137014.1"
                     /translation="MTYLTKPRLHHPDLPKNKLGYTHRDYEGTVSTLCAGCGHDSISA
                     AIIQAFFELDVAPHKVAKLSGIGCSSKTPTYFLGNSHGFNSVHGRMPSVLTGANLANR
                     DLIYLGVSGDGDSA"
     gene            complement(393..2243)
                     /locus_tag="VHT22_05360"
     CDS             complement(393..2243)
                     /locus_tag="VHT22_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX3137015.1"
                     /translation="MKPSAGPIQAVNDFVVKFANVNGSGSASANLLFAKAILRMGIPV
                     APRNIFPSNIQGLPTWFEVRVNAAGWRGRRGGVDMMVAMNPQTWDQDLKEIEPGGYLF
                     YDSTKPLPDAKFREHITVIGVPLTEICNREYSDARQRQLFKNIIYVGALSALLEIDPE
                     EIAHLIGDQYKGKEALLKPNVHAMTIGRSYAVNNLDCPLGIKVRRANAVGERIFIEGN
                     SAAALGAVYGGATVAAWYPITPSSSMAEAFARHCRKFRTEATSGKARYAIVQAEDELA
                     SIGIVIGAGWNGARAFTCTSGPGISLMQEFIGLAYFAEIPSVVFDVQRGSPSTGMPTR
                     TQQADVLSCAYASHGDTKHVLLFPEDPKEAFEFAALSFDLADRLQTPIFVMLDLDIGM
                     QDWLCEPLVWDDARTHDRGKVMSYEMLEAGKEFGRYLDADGDGIPYRTYPGTHPKRGS
                     YFTRGTTKDRYARYSEDGATYVDNMERLLRKFETAKSLVPEPARRDAGHATRFGVIYY
                     GSTAPAIDEALAALAGQGIHLDALRVRAFPFHDSIADFIAAHEQVFVVEQNRDAQLKS
                     LLVNELVVDPARLMPVLHYDGTPITARFIGSEIAEKLAMFNVTPLRKVAP"
     gene            complement(2240..4102)
                     /locus_tag="VHT22_05365"
     CDS             complement(2240..4102)
                     /locus_tag="VHT22_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3137016.1"
                     /translation="MKPTDIAAPDYFHKVVDCQWACPAHTPVPEYIRLISAGRYSDAY
                     MVNWESNVFPGILGRTCDRPCEPACRRSRVEEGQPDGSTIKPEPVAICRLKRVAADLK
                     DDIRERLPKLARRNGKRIALIGAGPASLTVARDLAPLGYQCVVYDGDARAGGMIRSQI
                     PKFRLPESVIDEEVGYVIDLGIDFRGGSRIDSLKRLLGEGYDAVFVGSGAPRGRDLDL
                     PGRREAAANIHIGIDWLSSVSFGHIEKIGKRVIVLGGGNTAMDCCRSSRRLGGTDVKV
                     IVRSGFDEMKASPWEKEDAMHEGIPIINYHVPKAFTHESGRLTGVVFEKVEARHDGDG
                     RRQLVPTGEPDVHFACDDVLIAVGQENAFPWIERDAGVDFDKSGLPVLDKKTLQSTLP
                     QVFFGGDAAFGPKNIIWAVAHGHDAAISIDRFCRGEDVAIRPPPLVNLMSQKMGIHEW
                     SYDNAISNDQRFKVPLKDVSIALKNIKVEVELGFDAAQAFAEAQRCLNCDVQTVFTSK
                     ACIECDACVDICPMDCITFTDDGDETDLRTRLNAPSRNLTQDLYVANGLKTGRVMAKD
                     EDVCLHCGLCAERCPTGAWDMQKFAFDIAQAGHRCHQPYANAVGARKPAARAAE"
BASE COUNT          755 a         1389 c         1388 g          830 t
ORIGIN      
        1 aggccgaatc cccatctccg gatacgccca gatagatcag gtcgcggttg gctaggttgg
       61 cacccgtcag caccgacggc atgcgaccgt gcacgctgtt gaagccatgc gaattgccga
      121 gaaaataggt cggagtcttc gaggagcagc ctatgccgga cagcttggcc accttgtgcg
      181 gcgcgacgtc gagctcgaag aaggcctgga tgatcgcggc cgagatcgag tcgtggccgc
      241 atccggcaca cagcgtcgac accgttcctt cgtagtcgcg atgggtgtag cccagcttgt
      301 tcttcggcag gtcgggatgg tggagcctgg gcttggtcag ataggtcata cgtcctctgt
      361 catgctgaat tcccgcgctg atcgatgctg cctcacggcg ccaccttgcg cagcggcgtc
      421 acattgaaca tcgcgagctt ttcggcgatt tcgctgccga tgaaacgggc agtgatcggc
      481 gtgccgtcgt aatgcagcac cggcatcagc cgggccggat cgacgaccag ttcgttgacc
      541 agcagcgact tgagctgcgc gtcgcgattc tgctcgacga caaagacctg ctcgtgcgcc
      601 gcaatgaagt cggcgatgct gtcgtgaaac gggaaagcgc gcacgcgcag cgcgtcgagg
      661 tgaataccct ggccggccag cgccgccagc gcctcgtcga tcgccggcgc ggtcgaaccg
      721 tagtagatga cgccgaagcg ggtcgcatgg ccggcatcgc gacgcgcagg ctccggtacg
      781 aggctcttgg cggtctcgaa cttgcgcagc aggcgttcca tgttgtcgac gtaggtcgcg
      841 ccgtcctcgc tgtagcgcgc gtatcggtcc ttggtcgtgc cgcgggtgaa atacgagccg
      901 cgcttcggat gcgttcccgg ataggtccga tacggaatgc cgtcgccgtc ggcgtcgaga
      961 tagcggccga actccttgcc cgcctccagc atctcgtaac tcatcacctt gccgcggtcg
     1021 tgcgtgcgcg catcgtccca gacgagcggt tcgcacagcc agtcctgcat gccgatgtcc
     1081 aggtccagca ttacgaaaat cggcgtctgc aggcggtcgg caaggtcgaa tgagagcgcg
     1141 gcgaattcga acgcttcctt cggatcttcg ggaaagagca gcacgtgctt ggtgtcgcca
     1201 tgcgaagcat aggcgcacga aagcacgtcg gcctgctgcg tccgcgtcgg catgccggtc
     1261 gacgggcttc cgcgctgaac atcgaacacc accgacggta tttcggcgaa ataggcgagc
     1321 ccgatgaatt cctgcatcag cgaaatgccc ggcccggacg tgcaggtgaa cgcccgcgcg
     1381 ccgttccagc ccgcgccgat cacgatgccg atcgaggcca gttcgtcctc ggcctgaacg
     1441 atcgcatacc gggccttgcc gctcgtagct tcggtgcgga acttgcggca atgccgcgcg
     1501 aacgcctcgg ccatcgacga cgagggcgtg atcggatacc acgcggccac cgtcgccccg
     1561 ccgtacacgg cgcccagcgc cgcggcgctg ttgccctcga tgaaaatacg ctcgccgacg
     1621 gcattggcgc gtctgacttt gatgcccagt gggcagtcga ggttgttgac cgcataactt
     1681 cgcccgatcg tcatcgcgtg cacgttgggc ttgagcagcg cctccttgcc cttgtactgg
     1741 tcgccgatca ggtgcgcgat ttcctcggga tcgatttcga gcagcgccga cagcgcgccg
     1801 acgtagatga tgttcttgaa cagctgccgc tgccgggcgt cggagtattc gcggttgcat
     1861 atctcggtca gcggcacacc gatgaccgta atgtgttcgc ggaatttcgc gtccggcaac
     1921 ggcttggtcg aatcgtagaa caggtagccg cccggctcga tctccttcag atcctgatcc
     1981 caggtctgcg gattcatcgc caccatcatg tcgacgccgc cgcgccggcc gcgccagccg
     2041 gccgcattga cgcggacctc gaaccacgtc gggaggccct ggatgttgga cggaaaaata
     2101 ttgcgcggcg caaccggaat gcccatgcgc aggatggcct tggcgaacag caggttcgcc
     2161 gatgcggagc ctgagccgtt gacgttggcg aacttgacga cgaaatcgtt gacggcctgg
     2221 atcgggccgg ccgaaggctt cattccgccg ccctcgccgc aggtttgcgc gcgccgaccg
     2281 cgttcgcgta cggctgatga cagcgatggc cggcttgtgc gatatcgaag gcgaacttct
     2341 gcatgtccca ggcgccggtc gggcagcgtt cggcgcaaag gccgcaatgc aggcacacgt
     2401 cctcgtcctt ggccatcacg cgcccggttt tcagcccgtt cgcgacatac agatcctggg
     2461 tcaggttgcg cgagggcgca ttcagccgcg tccggaggtc cgtctcgtcg ccgtcgtcgg
     2521 tgaaggtgat gcagtccatc gggcagatgt cgacgcaagc gtcgcattcg atgcatgcct
     2581 tcgaggtgaa gaccgtctgc acatcgcaat tcaggcagcg ctgcgcctcc gcgaacgcct
     2641 gcgccgcatc gaagccgagc tcgacctcga ccttgatgtt cttcaatgcg atcgacacat
     2701 ccttcagcgg aaccttgaag cgctggtcgt tcgaaatcgc gttgtcatag ctccactcgt
     2761 ggatccccat cttctgtgac atcaggttga ccagcggcgg gggccgtatc gcgacgtcct
     2821 cgccgcggca aaaccggtcg atcgagatcg cggcgtcgtg gccgtgcgcg accgcccaga
     2881 tgatgttctt cgggccgaac gccgcatcac cgccgaaaaa gacctgcggc agcgtcgatt
     2941 gcagcgtctt cttatcgagc accggcagtc cggacttgtc gaaatccacg ccggcgtcgc
     3001 gctcgatcca cggaaatgca ttttcctgtc cgacggcgat cagcacgtcg tcgcaggcga
     3061 agtggacgtc cggctcgccg gttggcacga gctggcggcg gccgtcgcca tcgtgcctcg
     3121 cttcgacttt ctcgaatacg acgccggtca gacgtccgct ctcgtgagtg aacgccttgg
     3181 gcacgtgata gttgatgatc ggaatgccct cgtgcatcgc gtcctccttt tcccaggggg
     3241 acgctttcat ctcgtcgaag cccgagcgca cgatgacctt gacgtcggtg ccgccgagcc
     3301 ggcgcgagct gcggcagcaa tccatcgcgg tgttgccgcc gccgagcacg atcacgcgct
     3361 tgccgatctt ctcgatgtga ccgaacgaaa cgctggacag ccagtcgatg ccgatgtgaa
     3421 tgttcgccgc ggcttcgcgg cgtccgggca gatcgaggtc gcgtccgcgc ggcgcgccgc
     3481 tgccgacgaa cacggcgtca tagccttcgc caagcagacg cttgaggctg tcgatgcgcg
     3541 agccgccgcg aaaatcgatg ccgagatcga tgacgtaccc gacctcctcg tcgatcaccg
     3601 actcgggcag gcgaaacttc ggaatctggc tgcggatcat gccgccggct cgggcgtcgc
     3661 cgtcgtacac gacgcactgg tagccgagcg gcgcgagatc gcgggcgacg gtcagcgacg
     3721 ccggtccggc gccgatcagc gcgatccgct tgccgttcct gcgcgccagc ttgggcaggc
     3781 gttcgcggat atcgtccttc aagtcggccg cgacccgttt gaggcggcag atcgcgaccg
     3841 gctcgggctt gatcgtgctg ccgtcgggct gtccttcctc gacccggctt cggcgacatg
     3901 ccggctcgca aggacgatcg caggtccggc ccagaatgcc cggaaagacg ttcgactccc
     3961 agttgaccat gtacgcgtcg ctatagcggc ccgccgagat caaccggatg tattcaggaa
     4021 cgggcgtatg ggcgggacac gcccactggc agtcgacgac cttgtgaaag tagtcggggg
     4081 ccgcgatgtc cgtcggtttc aatactgctc ctcggttcga atgcatagac acgcactcgg
     4141 atcggacgtg cgcacgatga ttcggtttgt tacttttttc ggtcgggcgt accggatatt
     4201 cggccgccaa gtggcgccga tactacgcct gtgtgacagc tatgaaaagg cctttgcttt
     4261 caactactag cagtatggct tttcatgtgg cctctaaaca acggcggaag gcaaagacag
     4321 ttcgagttct gcggcggcaa ttcaccgatt tgccgccgcg ct
//
LOCUS       DATANS010000370         1630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_734131, whole genome shotgun sequence.
ACCESSION   DATANS010000370 DATANS010000000
VERSION     DATANS010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734131"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1630)
                     /locus_tag="VHT22_05370"
     CDS             complement(<1..>1630)
                     /locus_tag="VHT22_05370"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX3137017.1"
                     /translation="GPPALPWSGTFHSIGARLLREYAERIGINPSFTIHDRGDSEDLL
                     AIVRHGLGLSATKNRFPGKGTCLAIYSRAVNSGDALSAVLAASFPWCAHWEAELNRLF
                     AAYAAEKGVQNALDYDDLLLYWAHMVAEPALGREIGARFDHVLVDEYQDTNRLQASIL
                     LGIKPDGAGLAVVGDDAQSIYSFRAATVRNILDFPHAFDPPACIVTLDRNYRSTQPIL
                     DASNAVIALASERYSKNLWTERKASQKPQLISVRDEADQARCVADQVLEHRECGMMLR
                     SQAVLFRSSSHSAQLELELARRGIPFIKFGGLKFLDAAHVKDVLCVLRWADNPRSRMS
                     GFRIARLLPGVGPATAGKLLDAMESAPDPVIAMGDFRMPGPAAADFDVLRDLFGALRS
                     AKASWPADIDAATRWYEPHLRRIYDDAHVRQADLIQLSRIASTYASRERFLTEVTLDP
                     PAATSDEAGAPGLDDDYLILSTIHSAKGQEWRSVHILNVVDGCIPSDLSTGSAEEIEE
                     ERRLLYVAMTRARDELRLMVPQRFYVHQQTQYGDR"
BASE COUNT          290 a          530 c          532 g          278 t
ORIGIN      
        1 cggtcgccat actgcgtctg ctgatgcaca taaaagcgtt gtggcaccat cagtcgcagc
       61 tcatccctgg cgcgggtcat cgcaacgtaa agcagtcgcc gttcctcctc gatctcttcc
      121 gccgacccag tgctcaagtc cgacgggatg cagccgtcga cgacattgag gatatgaacg
      181 ctgcgccact cctgtccctt ggccgagtga atcgtcgaaa ggatcagata gtcgtcgtcc
      241 aggccgggag cgccggcttc gtcgctggtc gccgccggcg ggtcgagcgt gacctcggtc
      301 aggaaacgct cgcgcgaggc ataggtcgat gcgatcctgc tcagttgtat caagtcggcc
      361 tgccgcacgt gcgcatcgtc gtagatccgg cgcaaatggg gctcgtacca gcgtgtcgcc
      421 gcgtcgatgt cggccggcca gctcgccttg gctgaacgga gcgcgccgaa caggtcgcgc
      481 aagacgtcga aatcggcggc ggcgggcccc ggcatgcgaa agtcgcccat cgcaatcacc
      541 ggatcaggcg cgctttccat cgcatcgagc agcttccccg cggttgccgg acccacgccg
      601 ggcagcaggc gtgcgatgcg aaagccggac atcctgctgc gcggattgtc cgcccagcgc
      661 aacacgcaca ggacatcctt cacgtgcgcc gcatcgagga atttgagccc gccgaacttg
      721 atgaatggaa tgccgcgccg ggccagctcg agctcgagct gcgcactgtg gctggagctg
      781 cggaacagca ccgcctgcga cctgagcatc attccgcact cgcggtgctc taggacctgg
      841 tcggcgacgc agcgcgcctg atcagcttcg tcgcgaacgc tgatcagctg cggcttctgc
      901 gatgccttgc gctcggtcca caggttcttg ctgtaacgct cgctggccag ggcgatgacc
      961 gcgttcgacg catcgaggat cggctgcgtc gaacggtagt tgcggtcgag ggtgacgatg
     1021 cacgcggggg gatcgaacgc gtgcggaaaa tccaggatgt tgcgcaccgt ggcagcacga
     1081 aacgaataga tcgactgcgc gtcgtcgccg acgacggcaa gcccggcgcc gtcgggcttg
     1141 atgccgagca ggatcgacgc ctgcaggcgg ttcgtatcct gatattcgtc gaccagcacg
     1201 tgatcgaatc gagcgccgat ctcccggccc agcgccggct cggcgaccat gtgcgcccaa
     1261 tagagcaaca ggtcgtcgta atccagtgcg ttctgcacgc ccttttcggc ggcatacgcc
     1321 gcgaacagcc tgttgagctc ggcttcccag tgcgcgcacc agggaaagct cgcggcgagc
     1381 accgcggaga gcgcgtcgcc gctgttgacg gctcgcgaat agatcgcgag gcaggttccc
     1441 ttccccggga agcgattctt ggtcgccgac aggccgaggc cgtgccgcac gatagcgagc
     1501 aaatcctccg agtcgccgcg gtcgtggatg gtgaacgaag gattgatgcc gatccgctcc
     1561 gcatattcgc gcagcagccg cgcaccgatg ctgtgaaatg tccccgacca cggcagcgcg
     1621 gggggacccg
//
LOCUS       DATANS010000371         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_145674, whole genome shotgun sequence.
ACCESSION   DATANS010000371 DATANS010000000
VERSION     DATANS010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145674"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="VHT22_05375"
     CDS             <1..1249
                     /locus_tag="VHT22_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX3137018.1"
                     /translation="DAVAAQHRAWSELRVQRDRAAASAQDREARLELLRYHLAELKAL
                     DLKAGELDELTAERQRLSQHGRLASGASEILQLLREAEDMNAEHAVSRALTVSRSLTE
                     LDPRFASIAKAIEESLIALREGIESAARYEADLEADPQRQEWVEQRLAAMETVARKHR
                     IEPAALGTLQTELATEFQQLDTLEASLEQIEKQLAQLQKQFHAACAKLSAARVAAAGK
                     LSRRISELMQTLGMPGGVFEIAVRALAADTANAHGADEVEFLVSANPGQPPRPLAKVA
                     SGGELSRISLAIQVAAAQSDALPCLVFDEVDAGVGGGVAEIVGRQLRTLGDRAQVLCV
                     THLPQVASQAHAHVRVTKLTDGKTTRTVLTVLGVDERVEEIARMLGGVSVTDKAREHA
                     AEMLKANGGRMKTRPARKSQAKA"
     gene            complement(1260..1694)
                     /gene="fur"
                     /locus_tag="VHT22_05380"
     CDS             complement(1260..1694)
                     /gene="fur"
                     /locus_tag="VHT22_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEX3137019.1"
                     /translation="METSDLRRAGLKITLPRLKILEILGTAQPRHMSAEDIYKHLLES
                     HEDIGLATVYRVLTQFEAAGLVTRHHFEGTTAVFELNEGEHHDHIVCLDCGKVEEFMD
                     AGIEERQRTVAGKMQFEIKDHSMILYGRCKRVNCPSREKDKR"
     gene            1811..2146
                     /locus_tag="VHT22_05385"
     CDS             1811..2146
                     /locus_tag="VHT22_05385"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEX3137020.1"
                     /translation="MTALFRLRRLAVAALVLTVLGACVHRIDIQQGNFLDKEDIDRIA
                     VGMTRVQVRALLGTPMVADPFQGSRWDYVYYFKRGRLHRAVQRHFIVYFDGADKVEKI
                     DRPEPAKKS"
     gene            complement(2148..2429)
                     /locus_tag="VHT22_05390"
     CDS             complement(2148..2429)
                     /locus_tag="VHT22_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEX3137021.1"
                     /translation="MRVWVVYALPDRQSVQELELPESATVAEAVARSGLLQKFPEIAE
                     RPRACAIYGRVVADSYSLRADDRVEILRPLQADPKERRRAAARDRTPKK"
     gene            complement(2437..>2718)
                     /locus_tag="VHT22_05395"
     CDS             complement(2437..>2718)
                     /locus_tag="VHT22_05395"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEX3137022.1"
                     /translation="HRAGMRELVTTRNTLTPPREITLALVAGPFKTLDGHWSFQPIGA
                     DRGTRVELTIRFEFANPMLNLLLARSFEKSCNELVDAFVARARAVYGTR"
BASE COUNT          498 a          885 c          879 g          456 t
ORIGIN      
        1 cgacgcggtc gccgcgcagc atcgcgcatg gtcagagctg cgcgtgcagc gcgaccgtgc
       61 tgccgcgtcg gcgcaggatc gtgaagcgcg gctcgaactc ctgcgctatc acctcgcaga
      121 gctcaaggca ctcgatctca aggcaggtga gctcgatgag ctgactgccg agcgacaacg
      181 actgtcgcag cacggccgac tggcatcggg tgcgagcgag atcctgcagc tcctgcgcga
      241 agcagaagat atgaatgcgg agcacgcggt ctcgcgcgcg ttgaccgttt cgcgctcgtt
      301 gacggagctc gatccgcgct tcgcttcgat cgccaaggcg atcgaggaga gcttgatcgc
      361 gctgcgcgaa ggcatcgaat ccgccgcgcg ttacgaagca gacctggaag ccgatccgca
      421 gcggcaggag tgggtcgagc agcgcctcgc ggcgatggaa accgtcgcac gcaagcatcg
      481 catcgagcct gcggctctcg gcacgctgca gacggagctc gcgacggagt ttcagcagct
      541 cgatacgctg gaagcatcgc tggagcagat cgagaagcag ctcgctcagc tgcagaagca
      601 gttccacgcc gcgtgcgcga aactcagtgc cgcacgcgtc gccgcagccg gcaagctctc
      661 gcgacgaatc agcgagctga tgcaaacgct cggcatgccc ggcggcgtgt tcgaaatcgc
      721 cgtacgcgcg ctcgcagccg atacagcgaa cgctcacggc gccgacgagg tcgaattcct
      781 ggtgagcgcg aaccccggcc aaccgccgcg tccgctcgcg aaggtggctt cgggcggcga
      841 gctgtcgcgc atcagcctcg cgatccaggt tgccgctgca cagtccgacg ccttgccgtg
      901 cctggtgttc gacgaagtgg acgccggcgt cggcggcggt gtcgcggaaa tcgtcggtcg
      961 tcagctacgc acgctcggcg accgcgcgca ggtactgtgc gtgacgcatc ttccacaagt
     1021 cgcgagccag gctcacgcgc acgtgcgcgt gacgaagctg acggatggca agaccacgcg
     1081 aacggtgctg acggtgcttg gtgtggatga gagagtggaa gaaattgcgc gcatgctcgg
     1141 cggtgtcagc gtgacggaca aagcgcgcga gcatgcggcg gagatgctga aggcgaacgg
     1201 gggccggatg aaaacccgac cagccaggaa gagtcaggct aaagcctgac ccacgagtcc
     1261 taccttttat ctttctcgcg cgacgggcaa ttcacgcgct tgcagcggcc gtacaggatc
     1321 atggagtgat ccttgatctc gaactgcatc ttgccggcga ccgtccgctg gcgctcttcg
     1381 atgcccgcgt ccatgaactc ttcgaccttg ccgcagtcaa gacagacgat gtggtcgtgg
     1441 tgctcgcctt cgttcagctc gaacaccgcg gtcgtgccct cgaagtggtg ccgcgtgacg
     1501 agcccggccg cctcgaactg cgtcagcacc cggtaaaccg tcgcgagccc gatgtcctcg
     1561 tgggattcga gcagatgctt gtaaatgtcc tcggcgctca tatggcgtgg ctgcgcggtg
     1621 ccaaggattt ccagaatctt gagccgcggc agcgtgatct tgagccccgc tctgcgcagg
     1681 tcgctggttt ccacgtgctt cactcctggg ggccggccac aaaaactagt atcatcgcgg
     1741 gccattatcg cccaccgtgc cgtcccgctc cacctttcat atctgcccaa tgaccgccgc
     1801 cagcaatgta atgacagcgc tcttccgtct tcgccggctc gccgttgccg ctctggtgct
     1861 caccgtgctc ggcgcctgcg tgcaccgcat cgacatccag cagggaaatt tcctcgacaa
     1921 ggaagacatc gaccgcatcg ctgtcggcat gacgcgggtg caggtgcgtg cgttgctcgg
     1981 cacgccgatg gtcgcggatc cgttccaggg ctcgcgctgg gactacgtgt actacttcaa
     2041 gcgcggccgg ctgcaccggg ccgtgcagcg ccatttcatc gtgtacttcg acggcgcgga
     2101 caaggtcgag aagatcgacc ggcccgagcc cgcgaagaaa tcgtaagtca cttcttcggc
     2161 gtgcggtcgc gcgccgcggc gcgacgcctc tccttcggat ccgcctgcag cggacgcaga
     2221 atctcgacgc gatcgtccgc ccgcaggctg tacgaatcag cgaccacacg accgtagatc
     2281 gcgcacgccc ggggacgctc ggcaatttcg ggaaacttct gcagcagccc ggagcgcgcg
     2341 acagcttcgg ctacggtcgc ggactcagga agctccagct cctgcaccga ctgccgatcc
     2401 gggagcgcat acacgaccca gacacgcatc gacgactcac cgcgtaccgt agacagcacg
     2461 cgcccgcgcg acgaaggcat cgaccagttc gttgcaactc ttctcgaagg agcgcgcgag
     2521 cagcaggttc agcatcgggt tggcaaactc gaagcgaatc gtcagctcga cgcgggtgcc
     2581 gcggtctgca ccgatcggct gaaacgacca gtggccatcg agtgtcttga aagggccggc
     2641 caccagcgcg agagtgattt ccctgggcgg agtcagcgtg ttgcgagtgg tcacgagctc
     2701 ccgcatcccg gcgcggtg
//
LOCUS       DATANS010000372         1695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_734241, whole genome shotgun sequence.
ACCESSION   DATANS010000372 DATANS010000000
VERSION     DATANS010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734241"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..85)
                     /locus_tag="VHT22_05400"
     CDS             complement(<1..85)
                     /locus_tag="VHT22_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HEX3137023.1"
                     /translation="MKVVLFCGGLGTRLREHSDTVPKPLVSI"
     gene            complement(111..1145)
                     /locus_tag="VHT22_05405"
     CDS             complement(111..1145)
                     /locus_tag="VHT22_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006543172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3137024.1"
                     /translation="MKILVTGSDGYIGARLVQRLINDGHDVTGFDTGLYRDGWLYSEQ
                     HATRRMPVTINKDLRNIEHGDVAGHDALVHLAELSNDPLGQNDPGVTHNINHLGSLKV
                     ARVAKEAGVPRFVYASSCSVYGKAVSDWVDETSPANPQTAYGECKVKVEQDVGPMADR
                     NFCVTFLRNATAYGASPRIRFDIVINDLCALAWTTKRIAMTSDGTPWRPVTHIDDICH
                     AIECTLAADPARVSGQIFNVGADSENYRIREIAEIIASVIPGCEVTIGQSAGDTRSYR
                     VSFKKIATQLPGFRAQWTARRGAEQLYELFKRIEMSKDTYEFRAFTRLKQLKYLKQTG
                     QVDDNLFWRY"
     gene            complement(1162..>1695)
                     /locus_tag="VHT22_05410"
     CDS             complement(1162..>1695)
                     /locus_tag="VHT22_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX3137025.1"
                     /translation="LFSGESVRAAETRTALARLLPADARFELELLQFRDGFFPMEWPA
                     IKERFEELKRRCNPDVIFTHHEHDRHQDHRTICELTWNTFRNHLVLEYEIPKYDGDLG
                     RPALFVPLSEQVVRHKIDTLLACYASQAGKRWFTAELFAGLMRVRGMECNAESGYAEA
                     FYARKLRLSFDGGQPHK"
BASE COUNT          285 a          549 c          536 g          325 t
ORIGIN      
        1 cgatgctcac cagcggcttc ggtacggtat cggagtgctc gcgcaaacgc gtgcctagac
       61 cgccgcagaa caacacaacc ttcatcgttt acccgccttt ggtccgacgc tcaatagcgc
      121 cagaacagat tgtcgtcgac ctgcccggtc tgcttcaggt acttgagctg tttgagccgc
      181 gtgaacgcgc ggaactcgta cgtgtccttc gacatctcga tgcgcttgaa cagctcgtac
      241 agctgctcgg cgccgcgtcg cgccgtccat tgcgcgcgga agcccggcag ctgcgtggca
      301 atcttcttga acgagacgcg atagctgcgc gtatcgccgg cgctctggcc gatcgtcacc
      361 tcgcagcccg gaatgacgct ggcaatgatc tcagcaatct cgcggatacg atagttttcg
      421 gagtcggcgc cgacgttgaa gatctggccg ctgacgcgcg ccgggtccgc cgccagcgtg
      481 cactcgatgg cgtggcagat gtcgtcgatg tgcgtgaccg gccgccacgg cgtgccgtcg
      541 ctggtcatcg cgatccgctt ggtcgtccac gccagcgcgc acaggtcgtt gatgacgatg
      601 tcgaagcgga tgcgcggcga ggcgccgtac gcagtggcgt tgcgcaggaa agtcacgcag
      661 aagttgcggt cggccatcgg gccgacgtcc tgctcgacct tcaccttgca ctcgccgtac
      721 gccgtctgcg ggttggccgg cgaggtctcg tcgacccagt cgctcactgc cttgccatag
      781 acgctgcagg acgacgcgta gacgaagcgc ggcacgcccg cctctttcgc cacccgcgca
      841 accttgagcg agccgaggtg gttgatgttg tgcgtcaccc cggggtcgtt ctggccgaga
      901 gggtcgttgg acagctcggc gaggtgtacc agcgcatcat ggccggcgac gtcgccatgc
      961 tcgatgttcc gcagatcctt gttgatcgtg accggcatgc gacgcgtcgc gtgctgctcc
     1021 gaatacagcc agccgtcgcg gtacaggccg gtgtcgaagc cggtcacatc gtgtccgtcg
     1081 ttgatcagac gctgcaccag gcgcgcgccg atatagccat ccgaaccggt caccaggatc
     1141 ttcatcgcct ctccagatgt gtcatttgtg tggctgcccg ccgtcaaaac tcagccggag
     1201 cttgcgcgcg taaaacgcct ctgcatagcc gctctcggca ttgcattcca taccgcgtac
     1261 gcgcatgagg ccggcgaaca gctcggccgt gaaccagcgc ttgcccgcct gcgacgcgta
     1321 gcacgcgagc agcgtatcga tcttgtgtcg cacgacctgc tcgctcaggg gcacgaacag
     1381 cgccggacgc cccaggtcgc catcgtactt ggggatctcg tattccagca ccagatgatt
     1441 gcggaaagtg ttccaggtca gctcgcagat cgtgcggtga tcctgatggc ggtcgtgctc
     1501 atggtgcgtg aagataacgt ccgggttgca gcggcgcttc agttcctcga agcgctcctt
     1561 gatcgcgggc cattccatcg ggaagaagcc gtcgcgaaac tgcagtaatt cgagctcgaa
     1621 gcgcgcgtca gctggcagca gccgtgcgag cgccgtgcgc gtctcggcgg cgcggacact
     1681 ctcgcccgag aacag
//
LOCUS       DATANS010000373         2641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_103072, whole genome shotgun sequence.
ACCESSION   DATANS010000373 DATANS010000000
VERSION     DATANS010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103072"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VHT22_05415"
     CDS             complement(<1..386)
                     /locus_tag="VHT22_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3137026.1"
                     /translation="MRPAHATLDHLVVAADTLGNGEDYLESVLGVRPQRGGKHAAMGT
                     HNSLLKLGERCFLEVIAIDPDAAAPPRPRWFDLDRPAMRALLAQQPRLIHWVARTDDI
                     ETASRASPIDPGPVHTMSRGSFRWRIT"
     gene            complement(386..769)
                     /locus_tag="VHT22_05420"
     CDS             complement(386..769)
                     /locus_tag="VHT22_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3137027.1"
                     /translation="MAIAGMNHFTILTDDVDNTVRFYGDFLGLEPGPRPNFNFPGAWL
                     YAGGEPILHVIGGRPKSELKPGVIDHMAFSGKDLAATLSALKARKVAYECRQQAGTGL
                     WQVFFFDPNGAKVELDFPASERVPG"
     gene            complement(793..1800)
                     /locus_tag="VHT22_05425"
     CDS             complement(793..1800)
                     /locus_tag="VHT22_05425"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX3137028.1"
                     /translation="MIAKLWAAAREWAARLFRRKPVEPGRFVADSKLAWRGWLAVMPW
                     LWPSREYLVYVPRAYGGWRRRPLLVLLHGCRQTPEDFAAATRIAALADSNGWLVLLPR
                     QSSKANAWTCWNWFDKATSAGRGEAAIVVAQIRAVRRAYRVHPRRIFVAGMSAGGCLA
                     AVLGLRHPKLFAGVAAHSAVACGAASGPMAAMQVLAHGADTAVEQIGAAARDAASRRA
                     LPVPLLVVHGGDDHVVSLRNARQLVRQYLVFNGRLDPKEMAPDELPPPDREITQQLSP
                     GRTATTIEYRDGARTLVRMVRVDGLGHAWSGGDATFPYNDPLPPDATALLGAFVADQI
                     R"
     gene            complement(1846..>2641)
                     /locus_tag="VHT22_05430"
     CDS             complement(1846..>2641)
                     /locus_tag="VHT22_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEX3137029.1"
                     /translation="EETSRILVDSRETFQKMQEFAGQYTQNILDRIEHYAGDRPLYDL
                     HGVEDEIQKALARRVDLKSGGYLIIDQTEAMTTIDVNTGGFVGGRSFDDTIFKTNLEA
                     AQVIARQLRLRNLGGIIIIDFIDMENADHRASVLAEFNKALERDRTRLTVNGFTQLGL
                     VEMTRKRTRESLAHVLCEPCVTCGGRGEIKTAQTICYEILREIVREARQFNARAFRIL
                     ASQSVIDLFLEEESQSLAQLGDFIGKPISLQVESIYTQEQYDIVLI"
BASE COUNT          427 a          873 c          895 g          446 t
ORIGIN      
        1 gtgatgcgcc agcggaagct gccgcgcgac atcgtatgca ccgggccggg atcgatcgga
       61 cttgcgcgac tcgccgtttc gatgtcgtcg gtgcgggcaa cccagtggat cagccgcggt
      121 tgctgcgcca agagcgcgcg catcgccgga cgatccagat cgaaccatcg gggccgcggc
      181 ggcgccgcgg cgtcggggtc gatcgcgatc acctcgagaa agcagcgctc gcctagcttg
      241 agcaggctgt tatgcgtgcc catagccgcg tgcttgccgc cgcgctgcgg gcggacgccg
      301 agtaccgact cgagataatc ttcgccgtta cccagcgtgt cggcggcgac gaccagatga
      361 tcgagcgtcg cgtgcgccgg gcgcatcaac ccggcacgcg ctcgctggcg gggaaatcga
      421 gctcgacctt ggcgccgttg gggtcgaaaa aaaagacctg ccaaagtccg gtgccggcct
      481 gctgtcggca ttcgtacgcg accttgcgcg ccttcagcgc cgacaaggtg gcggccagat
      541 ccttgccgct gaacgccatg tggtcgatca cgcccggctt gagctcgctt ttcggccgtc
      601 ccccgatgac atgcaatatc ggctcgccgc cggcatacag ccatgcgccg ggaaagttga
      661 aattcggccg cgggcccggt tcgaggccca ggaaatcgcc gtagaagcgc accgtgttgt
      721 cgacgtcgtc ggtgaggatc gtgaagtgat tcatgcctgc gattgccatt gcgtgtctcc
      781 ttgctgccgc atctaccgga tctggtcggc gacgaatgcg ccgagcagcg ccgtcgcatc
      841 gggcggcagt ggatcattgt acgggaaggt ggcgtcgccg ccgctccagg cgtggccaag
      901 accgtcgacg cgcaccattc gcaccaatgt gcgagcgccg tcgcgatact cgatcgtcgt
      961 cgccgtgcgt ccgggagaga gctgttgggt aatctcacgg tcgggcggcg gcagctcgtc
     1021 gggcgccatc tctttcgggt cgaggcgacc gttgaacacc aggtattggc gcacgagctg
     1081 ccgcgcattg cgcagcgaga cgacgtggtc gtcgcccccg tgcactacca gcaatgggac
     1141 cggcaacgcg cgccgggacg ccgcgtcgcg cgcggccgcg ccaatctgtt cgaccgccgt
     1201 atcggcgccg tgcgccagca cctgcatcgc ggccatcggt cccgacgccg cgccgcaggc
     1261 gacggccgaa tgcgcggcca cgccggcgaa gagcttcgga tggcgcaatc cgagcacggc
     1321 ggcaagacag ccgccggcgg acattccggc gacaaatatc cgccgcggat gcacgcggta
     1381 cgcgcgccgc acggcgcgta tctgcgcgac aacgattgcg gcttcgccgc ggccggcgct
     1441 ggtcgccttg tcgaaccagt tccagcaggt ccaggcatta gccttgctcg actgccgcgg
     1501 cagcaggacc agccatccgt tgctgtcggc gagcgcggcg attcgcgtgg ccgcggcgaa
     1561 atcctcgggc gtctgccggc agccgtgcag cagcaccagc aacggccgcc gcctccagcc
     1621 gccgtatgcg cggggcacgt agaccaggta ttcgcgcgac ggccaaagcc agggcatgac
     1681 cgccagccag ccgcgccacg cgagcttgct gtcggcgacg aaacgaccgg gctcgacagg
     1741 cttccgccgg aacaggcgcg cagcccattc acgcgcggcg gcccagagtt tggcgatcat
     1801 cggtgcgatt gcggcgcggc gcttgcgcgg gcgcgccagc ggacgctaga tcagcacgat
     1861 gtcgtactgt tcctgcgtgt agatcgactc gacctgaagg gaaatcggct taccgatgaa
     1921 atcgccgagc tgcgcgaggc tctgcgattc ctcctcgagg aacagatcga tcaccgactg
     1981 cgaggcgagg atgcgaaagg cccgcgcgtt gaactgcctg gcctcacgca cgatctcgcg
     2041 caggatctcg tagcagatcg tctgcgcggt cttgatctcg ccgcgtccgc cgcaggtgac
     2101 gcacggctcg cacagcacgt gcgcgaggct ctcgcgcgtg cgcttgcgcg tcatctcgac
     2161 caggccgagc tgggtaaacc cattgacggt cagccgggtg cggtcgcgtt ccagtgcctt
     2221 gttgaattcg gccagcaccg aggcgcggtg gtcggcgttc tccatgtcga tgaagtcgat
     2281 gatgatgatg ccgccgagat tgcgcaggcg cagctgccgg gcgatcacct gcgccgcctc
     2341 gagattggtc ttgaagatgg tgtcgtcgaa gctgcggccg ccgacgaagc cgccggtgtt
     2401 gacgtcgatc gtcgtcatcg cctcggtctg gtcgatgatc agatagccgc ccgacttgag
     2461 gtcgacgcgc cgcgccagcg ccttctggat ctcgtcctcg acgccgtgca gatcgtacaa
     2521 cggccggtcg ccggcgtaat gctcgatgcg atcgagtatg ttttgcgtgt actggccggc
     2581 gaattcctgc attttctgga acgtctcgcg cgaatcgacc aggatccggg atgtctcctc
     2641 g
//
LOCUS       DATANS010000374         3052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_734385, whole genome shotgun sequence.
ACCESSION   DATANS010000374 DATANS010000000
VERSION     DATANS010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734385"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VHT22_05435"
     CDS             complement(<1..349)
                     /locus_tag="VHT22_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137030.1"
                     /translation="MMTDRIDAAAPAAQAQAWIAGVGLLVAGIAVSVLLVAIVHSGGN
                     WRGGPPAVAVDGGAFVLGRGRGHRDGREFVLEATDGEGLAVLSARVAPFGADGHPRID
                     FNLHSTDALPVDLV"
     gene            435..2042
                     /locus_tag="VHT22_05440"
     CDS             435..2042
                     /locus_tag="VHT22_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3137031.1"
                     /translation="MGDTQQTAVIVGGGPAGLTAALELIRHTNVRPIVIEAGDAVGGI
                     SRTIDCGGYRIDLGGHRFFSKSDWVMNWWQEILPLDRESRPADEPISVGYRNRHRTVP
                     AAAASGAGTRGDLVMLVRHRLSRIYFRRKFFDYPVKLNLRTIANLGARRFAKAAASYA
                     WATFFPRHPERSLEDFLINRFGVELYRTFFKSYTEKVWGVSCDRISAEWGAQRIKGLS
                     LVAAVRHALSRAAPRSTLPSRRTRTSLVEHFLYPKLGPGQLWEEVARQVRAGGGEIRL
                     SQRIERIRLTGNRVAGVDVRDVMTGALGSMPADYVISSMAVQDLVACLDPVPPAEVRR
                     VASELPYRDFITVGLLVTRMKSRSWTGTGSDAAFPPDNWIYIQEPDVRAGRLQIFNNW
                     SPAMVADPALIALGVEYFCNRDDELWSLSDAKLEALAIRELQQIDLIDGNNVVKAAVV
                     RVPRAYPAYFGAYAEFGVIRAHVDTIENLFLVGRNGMHRYNNQDHSMLTAKAAIDNIA
                     SGRTDKQNIWDINVDDEYHEEREPASA"
     gene            complement(2078..2476)
                     /locus_tag="VHT22_05445"
     CDS             complement(2078..2476)
                     /locus_tag="VHT22_05445"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX3137032.1"
                     /translation="MSDLLFHMIAGAPDPVAPFSHAVEADGWVFVTGQMPFEGRSNTS
                     PYPATIEEQTHQVMKNLVAVLKGCGLGLADVAAARVYLLHFDDDYARMNEVYAGYFPE
                     TRRPARTCIGVTGLARGARIEIDMIARRTS"
     gene            2525..2920
                     /locus_tag="VHT22_05450"
     CDS             2525..2920
                     /locus_tag="VHT22_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX3137033.1"
                     /translation="MRPIPVGAKGSFSLVVRPEHLANRFKDATLPPVLATPVMIMMME
                     NAALNAIKPYLDAGESAVGTRVDVQHLAATPAGRTVSAEAEVTRVTGKQVAFRIDATD
                     GVQRIGTGTHERMVIELDRFIERLNRKPG"
BASE COUNT          548 a         1013 c          981 g          510 t
ORIGIN      
        1 aaacgaggtc caccggcaac gcgtcggtcg agtgcaggtt gaagtcgatg cggggatgac
       61 cgtcggcgcc aaaaggcgcg acccgcgccg agagcacggc cagcccctcg ccgtcggtcg
      121 cttcgagcac gaactcccgg ccatcccggt gtccccggcc gcggcccagc acgaacgcac
      181 caccgtcgac cgctaccgcc ggcggaccgc cgcgccaatt gccacccgaa tggacgatgg
      241 cgaccagcag caccgacacc gcgatgccgg cgacgagcaa gccgacgccg gcaatccacg
      301 cctgcgcttg cgcggcgggc gctgccgcat cgatgcgatc agtcatcaag cggacactag
      361 catccgcagc gatcgattgc cacatccgag gcggcgtact gcggatgacg ccgcatcccc
      421 tacaatgcgc acccatgggg gacacgcaac agaccgctgt catcgtcggc ggcgggccgg
      481 cgggactcac cgctgcattg gagcttatcc gacatacgaa cgtccgcccg atcgtcatcg
      541 aggccggcga tgccgtcggc ggcatctcgc gcacgatcga ctgcggcggc tatcgtatcg
      601 accttggtgg ccatcgcttt ttttcgaagt ccgattgggt gatgaactgg tggcaggaaa
      661 tcctgccatt ggaccgtgaa tcgcgccccg cggacgagcc gatcagcgtc ggctaccgca
      721 accgtcaccg caccgtgccc gccgccgccg cgagcggtgc ggggacgcgc ggcgatctgg
      781 tgatgctggt gcgccaccgt ttgtcgcgaa tctatttccg gcgcaaattc ttcgactatc
      841 cggtcaagct caacttgcgc acaatcgcca acctaggcgc gcggcgcttt gccaaggctg
      901 cggcgagcta cgcctgggcg acgttttttc cacgccatcc cgagcgcagc ctcgaggact
      961 tcctgatcaa ccgcttcggc gtcgagttgt atcgcacgtt cttcaagtcg tacaccgaaa
     1021 aggtctgggg tgtctcctgc gaccggatca gtgccgaatg gggagcgcaa cgcatcaagg
     1081 gactttctct ggttgccgcg gtgcggcacg cgttgtcgcg cgcggcgccg cgatcgacgc
     1141 ttccgtcgcg ccggacgcgc accagcctgg tcgaacactt tctatacccg aagctcggtc
     1201 cgggccagtt atgggaggaa gttgcgcgcc aggtccgggc cggtggcggc gagattcgct
     1261 tgtcgcagcg catcgagcgg atccggctta cgggaaatcg agtggccggg gtcgacgtac
     1321 gggatgtcat gaccggggcg ctcggcagca tgccggcgga ttacgtcatt tccagcatgg
     1381 ccgtccagga cctggtcgca tgcctcgatc cagtgccgcc ggccgaagta cgccgcgtag
     1441 cgagcgagct gccctaccgc gatttcatta cggtgggtct gctcgtgacc cggatgaaaa
     1501 gccgcagctg gacgggcacc ggcagcgatg ctgctttccc gcccgacaac tggatctaca
     1561 tccaggagcc cgacgtccgc gccggccggc ttcagatctt caacaactgg agtcccgcga
     1621 tggtcgccga tccggcgctg atcgcgctgg gcgtcgaata tttctgcaac cgggatgacg
     1681 agctctggtc gctgtccgac gcaaagctcg aggcgctcgc catccgcgag ctgcagcaga
     1741 tcgacctgat cgacggcaac aatgtagtca aggctgccgt cgtgcgtgtg cccagagcct
     1801 accccgcgta cttcggcgcc tacgcggagt tcggcgtcat ccgggcgcac gtcgacacga
     1861 tcgagaacct tttcctggtc ggccgcaatg gcatgcatcg ctacaacaat caggatcact
     1921 cgatgctcac cgcgaaggcg gcgatcgaca acattgcttc cggtcgcacc gacaagcaga
     1981 atatctggga catcaatgtc gacgacgaat accacgagga gcgcgaacca gcgtcggctt
     2041 gaccgggcca ccgctcggcc ggagccgttc agggagttca gctggtgcgg cgcgcgatca
     2101 tgtcgatttc gatgcgggcg ccgcgtgcga gaccggtgac gccgatgcac gtgcgcgctg
     2161 gccggcgggt ctcgggaaag tagccggcat aaacctcgtt cattcgcgcg tagtcgtcgt
     2221 cgaagtgcag cagatagacg cgtgctgccg cgacgtccgc caagcccagc ccgcagcctt
     2281 tcagcacggc aacgaggttc ttcatcacct ggtgagtctg ctcttcgatc gtcgccggat
     2341 aaggcgatgt gttcgacctg ccttcgaagg gcatctgccc ggtgacgaac acccagccat
     2401 cggcttcgac ggcatgactg aacggcgcga ccggatcggg agcgccggct atcatgtgaa
     2461 acaacaggtc ggacatcggc ttgggtcgct ttatgcgtca ttctaccgat cgaggaggac
     2521 aacaatgcgg ccgatacccg tcggggccaa gggctcgttt tcgctggtcg tcaggcccga
     2581 gcacctggcc aaccgcttca aggacgcaac gctgcccccg gtgctcgcga cgccggtgat
     2641 gatcatgatg atggaaaacg ccgcgctcaa cgccatcaag ccctacctcg atgccggcga
     2701 aagcgcggtc ggaacacggg tcgacgttca gcacctggcg gccactcccg ccgggcgcac
     2761 cgtctccgcc gaggcggagg tgacgcgggt cacgggcaag caggtggcgt ttcgcatcga
     2821 cgcgaccgac ggcgtacagc gtataggcac cggcacgcac gagcgcatgg tcatcgagct
     2881 cgaccgattc atcgaacggc tgaatcggaa gcccggctag gcgccggatg cgcgcgtcac
     2941 cggtgttcgt tgccgcgggt gattgcgcgg tgtcggaacc gccgacgggc cgcgctcggc
     3001 gggttcctcc gccagcacgc cgatccaatg ctgcaacgcc agtcggccat gc
//
LOCUS       DATANS010000375         2054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_103108, whole genome shotgun sequence.
ACCESSION   DATANS010000375 DATANS010000000
VERSION     DATANS010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103108"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /gene="gatB"
                     /locus_tag="VHT22_05455"
     CDS             <1..481
                     /gene="gatB"
                     /locus_tag="VHT22_05455"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817728.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEX3137034.1"
                     /translation="AMLVSQREMASYFERLVAAGVDPRLAGNWLLGEVSAALNKAEID
                     IVEAPVSSASLAGMLRRIADGTISGKLAKQVFEAMWAGEGDADAIIAARGLRQISDAG
                     AIEQIVADVLAANAAIVAEYKAGKEKAFQSLVGKAMAATRGKANPIEVNAALKKMLG"
     gene            complement(569..1204)
                     /locus_tag="VHT22_05460"
     CDS             complement(569..1204)
                     /locus_tag="VHT22_05460"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX3137035.1"
                     /translation="MTITVYHLETSRSERIVWLMEELDLPYELQVFPRETTGAAPHPL
                     KEIHAMGKAPVIRDGDTVLAESGAIVDYIVHRHGGGRLALSPDDPAYAKYIYWLHFAE
                     GSLMSLMLIALVLSRVKEAAESPVTARIRERMKQMLTFVDTELGAGPWFAGDAFTAAD
                     VMMVFPFTTLRNFLDYDLAQYANIVAYVARVGVWPAYRKAMSLAGPTRSYL"
     gene            1357..>2054
                     /locus_tag="VHT22_05465"
     CDS             1357..>2054
                     /locus_tag="VHT22_05465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3137036.1"
                     /translation="MIPPDFERALDNPFWSSLTTGHAHFAQGGTLARRYPADISPIAG
                     LAAPERANVTALETVVGVGDDMATVARLLPKLPPHWEILYESRITQMIRSDRKSLPEG
                     DLDATTLDAADVPEMLRLVELTKPGPFRPRTIELGTYIGARLSGRLVAMAGERLWIDD
                     LREVSAVCTHPDVQGRGYARALIGRVVNRMLREDQTPLIHVESTNKRAIELYLTLGFV
                     RRAELGLLHAKRTR"
BASE COUNT          391 a          629 c          688 g          346 t
ORIGIN      
        1 agcaatgctg gtttcgcagc gggaaatggc gagctatttc gaacggctcg tcgctgcagg
       61 cgtcgatcca cggctggcgg gcaactggct gctcggcgaa gtgtcggctg cgctcaacaa
      121 ggcggaaatc gacatcgtgg aggcgccggt atcctcggcc tcgctagccg gcatgctgag
      181 acggatcgcc gatggcacga tctcgggcaa gctcgccaaa caggtgttcg aagcgatgtg
      241 ggcgggcgaa ggtgacgccg acgcgataat cgcggcccgg ggcctgcggc agatttccga
      301 cgcgggcgcc atcgagcaga tcgtcgccga cgtgcttgca gccaacgcgg cgatcgtcgc
      361 cgagtacaag gccggcaagg agaaggcgtt ccagtcgctg gtcggcaagg cgatggccgc
      421 gacccggggc aaggcgaatc cgatcgaggt caatgcagcg ctgaagaaaa tgctcggctg
      481 aaatccgcgc gccaaggcgg ctgagcgcgc cgccggcaaa tcgaaccgca cggcgtcaac
      541 gcgcacgcac cgagagattt gacccgggtt acaggtaact tctggtcgga cccgcgagcg
      601 acatcgcctt cctgtacgcc ggccacaccc cgacgcgcgc gacgtaggcg acgatgttgg
      661 cgtactgtgc gagatcgtag tcgaggaagt tccgcagcgt cgtgaaggga aacaccatca
      721 tcacgtcggc ggcggtgaaa gcgtcgccgg cgaaccacgg cccggcgccg agctcggtgt
      781 cgacaaaggt cagcatctgt ttcatccgct cgcggattcg cgccgtgacc gggctttccg
      841 cagcctcctt cacgcggctc aacaccagcg cgatcagcat cagcgacatc aggctgcctt
      901 cggcgaaatg cagccagtaa atgtacttcg cgtacgccgg atcgtcgggc gacagcgcca
      961 gccgcccgcc cccgtggcga tgcacgatat agtcgacgat cgctccggac tcggcgagca
     1021 cggtgtcacc gtcgcggatc accggcgcct tgcccatagc gtggatttcc ttcaacggat
     1081 gcggtgccgc gccggtcgtc tcgcgcggaa agacctgcaa ttcataggga agatcgagct
     1141 cctccatcag ccagacaatg cgctcggagc gcgaggtctc cagatgatag acggtgattg
     1201 tcatcgtggg ctcccgtcga aacgggaaag agtttgcagc ggtttgatct cggccgcaat
     1261 cgcgagcggc cgccgacggg cgacgacgtg gcaaggtcgc gttaaattgt gcactcgatc
     1321 tgcccgaagg ccgcatcacg tgacaaaatt cccgggatga tcccgcctga tttcgagcgc
     1381 gcgctcgaca acccgttctg gtcgagcttg acgactggac atgcgcattt cgcgcagggc
     1441 gggacgctgg cgcgcagata cccggccgac atttcgccta tcgccggatt ggctgcgccg
     1501 gaacgcgcga acgtgactgc actggagacg gtggtcggcg tcggtgacga catggcaacc
     1561 gtcgcgcggt tgctgccgaa gcttccgcct cattgggaga ttctgtatga atcgcgcatc
     1621 acccagatga ttcggagcga tcggaagtcg ttgccggagg gcgatttaga tgcaacgact
     1681 ctcgacgcgg cggacgttcc ggaaatgctt cggctggtcg agctcaccaa gccgggaccg
     1741 tttcgaccgc gcaccatcga gctcggaacg tatatcggcg cgcggctgtc ggggcgcctg
     1801 gtcgcgatgg cgggcgagcg gctgtggatc gacgatttgc gcgaggtcag cgccgtatgc
     1861 acgcatcccg atgtgcaagg acgcgggtac gcgcgggcgc tgatcggccg ggtcgtcaat
     1921 cgaatgctgc gcgaggacca gacgccgttg attcatgtcg agagcaccaa caagcgcgcg
     1981 atcgagctct acctaacgct cggttttgtc cgccgcgcgg agcttgggct gcttcacgcg
     2041 aaaaggaccc gata
//
LOCUS       DATANS010000376         2407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_355728, whole genome shotgun sequence.
ACCESSION   DATANS010000376 DATANS010000000
VERSION     DATANS010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355728"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VHT22_05470"
     CDS             <1..444
                     /locus_tag="VHT22_05470"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3137037.1"
                     /translation="IAVAGATYDLDDDDRYPNAVSHAVNLARAEHMLPGSAAGIDAAT
                     LDGGVGFRCVATDRLPMVGAIVAIDRARADQAALAGAHLADLPRIRGLYGAFAFASRG
                     LSWSALAAEALASELDAEPAPLSHALLDAIDPGRFVVKALRHGAL"
     gene            493..1359
                     /locus_tag="VHT22_05475"
     CDS             493..1359
                     /locus_tag="VHT22_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX3137038.1"
                     /translation="MPAPIATLLKPNTRDIGGLLVQRLLPAFPTQMVGPFIFFDHFGP
                     TVFKSGNGLDVRPHPHIGLATVTYLFEGGQVHRDSLGNVQAIVPGDVNWMTAGQGIVH
                     SERTGPKDRAAGHRLHGLQTWVALPKSHENVEPSFSHHPRASLPEIEEPGVTMRLLAG
                     TAFGRRAPTPTFSPMIYLAIDLAAGGGVDLPPEHQERGVYVIDGDVEVGGVPAPVGHL
                     AVLASDLTARIEAKSASRLVVLGGAKMDGARLIWWNFVASSKELIDSASARWREQAYP
                     PVPDETEFIPLP"
     gene            complement(1469..>2407)
                     /gene="metK"
                     /locus_tag="VHT22_05480"
     CDS             complement(1469..>2407)
                     /gene="metK"
                     /locus_tag="VHT22_05480"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX3137039.1"
                     /translation="GYTDPELRFDAQGCAVMVCYGRQSPDIARGVDRTSDEYMNQGAG
                     DQGLMFGYACDETPALMPFPIYYAHRLVQRQSEVRRDGRLPWLRPDAKSQVTVRYTGG
                     KPSSIDTVVLSTQHHPEMNHKQEQLAEAVVEEIIKPVLPAEMLVNTRYLVNPTGRFEI
                     GGPHGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAARYVAKNIVASGI
                     ARQCQVQLSYAIGVAKPINVTVYTEGTGKIPDEKIAALVTDNFDLRPKGIIQMLDLLR
                     PIYLKTAAYGHFGRDEPEFTWENVDRADALRAAAGL"
BASE COUNT          376 a          827 c          784 g          420 t
ORIGIN      
        1 atcgccgtcg cgggcgcgac ttacgatctc gacgacgacg atcggtaccc gaacgccgtg
       61 agccacgccg tcaacctcgc ccgcgccgaa cacatgctgc ccggcagcgc cgccggcatc
      121 gatgcggcaa cgctcgacgg cggcgtcggt tttcgctgcg tcgcgaccga ccgcctgccg
      181 atggtcggcg cgatcgtcgc catcgatcgc gcgcgcgccg atcaggccgc gctcgccggc
      241 gcccatcttg ccgatctgcc gcgcatccgc ggcctgtacg gcgcgttcgc cttcgcctcg
      301 cgaggcctgt cgtggtcagc gctggcggcg gaggcgcttg cgagcgagct cgatgccgag
      361 cccgcgccgc tttcgcacgc gctgctcgat gcgatcgatc cgggccgctt cgtcgtcaag
      421 gcgcttcggc acggcgcgct ttagcgcgag cctcgaagcg atacaataca ggtgcagcac
      481 ttaagacgcg ccatgcccgc cccgatagcc acactgctca agccgaatac gcgcgacatc
      541 ggcggccttc tggtgcagcg actgctgccg gcttttccga cccagatggt cggaccgttc
      601 atttttttcg atcatttcgg tccgaccgta ttcaagtccg gcaacggcct cgacgtgcgc
      661 ccgcatccgc acatcgggct cgccaccgtc acctaccttt tcgaaggcgg gcaggtccac
      721 cgcgacagcc tgggcaacgt gcaggcgatc gttccgggcg acgtcaactg gatgaccgcc
      781 gggcaaggca tcgtacactc ggagcgcacg ggccccaagg accgcgccgc gggccaccgg
      841 ctgcatggac tgcagacgtg ggtcgcgctg ccgaaatcac acgagaacgt cgaaccttca
      901 ttcagtcatc atcccagggc gtcgcttccg gagatcgaag agccgggcgt gacgatgcga
      961 ctgcttgccg gcacggcatt cggccggcgt gcgccgacac cgacgttttc cccgatgatc
     1021 tatctggcga tagaccttgc ggccggtggg ggcgtcgatc tgccacccga gcaccaggag
     1081 cgcggcgtgt atgtgatcga tggcgacgtc gaggtcggcg gcgtgccggc gccggtcgga
     1141 caccttgccg tgctcgcgtc cgacctgact gcgcgcatcg aagcgaagtc ggcgtcgcgg
     1201 ctggtcgtgc tcggcggcgc gaagatggac ggcgcgcggc tcatctggtg gaacttcgtg
     1261 gccagctcca aggaactgat cgacagcgcg agcgcgcgct ggcgtgagca ggcttacccg
     1321 ccggtgcctg acgaaacgga attcatcccc ttgccctgac ccggaatcac aagcccgccg
     1381 ccgcgcgcaa cgcatccgcg cttggttaga ttggctccgg ccgcgcgcgc ccgctgggcg
     1441 cgcgcaccct gcgccgaaac atcgatcgct acaagcccgc cgccgcgcga agcgcgtcgg
     1501 cgcggtcaac attttcccag gtgaactcgg gctcgtcgcg gccgaaatgg ccgtaggcgg
     1561 cagtcttcag ataaatcggc cgcagcaggt cgagcatctg gatgatgccc ttcggtcgca
     1621 agtcgaaatt gtcggtcacc agagcggcga tcttctcgtc cggaatcttg ccggttccct
     1681 cggtgtagac ggtgacgttg atcggcttcg cgacgccgat cgcgtacgag agctgcacct
     1741 ggcattgccg cgcgatgccg gacgcaacga tgttcttggc cacatagcgc gcggcgtagg
     1801 ccgccgagcg gtctaccttc gacggatcct tgccggaaaa tgcgccgccg ccgtgcggcg
     1861 ccgccccgcc gtaggtatcg acgatgatct tgcgtccggt caagccggca tcgccgtgcg
     1921 gaccgccgat ctcgaagcga ccggtcggat tgaccaggta gcgcgtattg accagcatct
     1981 ccgccggcag gaccggcttg ataatctcct cgacgacggc ttcggcgagt tgctcctgct
     2041 tgtggttcat ttccggatga tgctgggtcg acagcacgac ggtgtcgatg ctgcttggct
     2101 tgccgccggt gtagcgcacc gtcacctgcg atttcgcgtc gggccgaagc cacggcagcc
     2161 gtccgtcgcg gcgaacttcg ctctgcctct gcaccaggcg gtgcgcgtaa tagatcggga
     2221 acggcatcag cgccggcgtc tcgtcgcatg catagccgaa catcaggccc tggtcgcccg
     2281 ccccctggtt catgtattcg tcggacgtgc ggtcgacgcc gcgcgcgata tcgggcgact
     2341 ggcggccgta gcacaccatt accgcgcatc cttgggcatc gaagcgcaat tcggggtcgg
     2401 tgtagcc
//
LOCUS       DATANS010000377         2654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_523741, whole genome shotgun sequence.
ACCESSION   DATANS010000377 DATANS010000000
VERSION     DATANS010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523741"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VHT22_05485"
     CDS             complement(<1..616)
                     /locus_tag="VHT22_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3137040.1"
                     /translation="MFSNKVTAVAFAAIASMLSATAFARDLAVVSWGGAYQDAQREVY
                     FKPFMEKTKVNMTEENWDGGVGTLRAKIQGGNNNWDVVQVESEELLLGCEEGLYEKID
                     WAKLGGKDKYLPDAVNDCGVGAIVYSFILAYDGDKIKGDVPKSWADFWNVKKWPGKRA
                     LRKGAKTTLEIALMADGVAPKDVYKTLGTPAGVDRAFKKLDEIKP"
     gene            complement(632..1741)
                     /locus_tag="VHT22_05490"
     CDS             complement(632..1741)
                     /locus_tag="VHT22_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008365238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3137041.1"
                     /translation="MNPSEPIVRFVGVEKSYDGITRVVDDLDLDIERGEFLTLLGPSG
                     SGKTTTLMLLAGFETPTGGQILLEGKSLSHMPPHQRNIGMVFQNYALFPHMSVADNIG
                     FPLSVRGVPVAEIGARVDRALDMVQLSGFGGRRPAQLSGGQQQRVAVARALVFEPKLI
                     LMDEPLGALDKQLREQMQLEIRRLHQRLGVTMVYVTHDQSEALTMSDRIAVFHRGRIQ
                     QLDTPKEMYEYPQNAFVARFIGENNRLDGRTEATADGRCMIRIKDSDARIEGTLVEPV
                     QDGGAVTVSLRPERVQIHLAGHTIDQPRGCSLAGTLREIVYLGDHVRARVALSGSEEF
                     TVKRPISEAHAIPDIGAAVEMFWEPEHCRVFAQEN"
     gene            complement(1819..>2654)
                     /locus_tag="VHT22_05495"
     CDS             complement(1819..>2654)
                     /locus_tag="VHT22_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3137042.1"
                     /translation="YYYRGARAGESEEAFAERLAANLEALIVHEDPNTVAAFFAEPVM
                     GAGGVIVPPATYFDRIQPILKKYDILFVVDEVICGFGRTGNLFGSQTFNLEPDMITVA
                     KALSAGYLPISATMVSNALYDILLAQSDKLGIFGHGYTYSSHPVPAAVALETLKIYAE
                     RDIVGAVRRLGPRLQAAIRSYAAHPLIGDARGIGLIGAVELVRDKATRESFAPKAGVA
                     AHLVRRAQHHGAIFRNMPGDIVAFSPPLIISEAEVDDMMACFGKALDDTWAMVKEKGL
                     A"
BASE COUNT          457 a          877 c          824 g          496 t
ORIGIN      
        1 tcggcttgat ctcgtcgagc ttcttgaacg cgcgatcgac gccggccggc gtgccgagcg
       61 tcttgtacac gtccttcggc gcgacgccgt cagccatcag cgcgatctcg agcgtcgttt
      121 tcgcgccctt gcgcagcgcc cgcttgcccg gccatttctt cacgttccag aaatcggccc
      181 aggatttcgg cacgtcgcct ttgatcttgt cgccgtcgta ggcaaggatg aagctgtaga
      241 cgatcgcgcc gacgccgcaa tcgttgaccg cgtcgggcag gtacttgtcc ttgccgccga
      301 gcttggccca gtcgatcttt tcatacaggc cttcctcgca gccgagcagc agctcctcgg
      361 actcgacctg gacgacatcc cagttattgt tgccgccctg gatcttggcg cgcagcgtac
      421 cgacgccacc atcccagttt tcctcggtca tgttgacctt ggttttttcc atgaacggct
      481 tgaaatagac ctcgcgctgc gcatcctgat aggcgccgcc ccacgacacg acagccaggt
      541 cgcgcgcaaa ggccgtcgcc gacagcatcg atgcaatcgc ggcgaacgca acggcagtta
      601 ccttgttgct gaacatggtg ctctcctcaa cctagttttc ctgcgcaaag acgcggcagt
      661 gttcgggttc ccagaacatt tccaccgccg cgccgatatc gggtatcgcg tgcgcctcgc
      721 tgatcggacg ctttaccgtg aactcctcgc tcccactcag cgccacgcgc gcacggacgt
      781 ggtcgccgag atagacgatc tcgcgcagcg tgccggcaag gctgcagccg cgcggctggt
      841 cgattgtatg cccggccaga tggatttgca cccgctccgg gcgcagcgaa acagtgacgg
      901 cgccgccgtc ctgcacaggc tcgaccagcg tgccttcgat ccgggcgtcg ctgtccttga
      961 tccggatcat gcagcggcca tcggcggtcg cctcggtcct gccgtcgaga cggttgttct
     1021 cgccgatgaa gcgggcgacg aaggcgtttt gcgggtattc gtacatctct ttgggcgtgt
     1081 ccagctgctg gatccggccg cggtggaaca ccgcgatccg gtccgacatc gtcagcgcct
     1141 cggactgatc atgggttacg tagaccatcg tgacgcccag gcgctggtgc agccgccgga
     1201 tctcgagctg catctgctcg cgcagctgct tgtcgagcgc gcccaacggc tcgtccatca
     1261 gaatcagctt gggctcgaac accagcgcgc gggcgaccgc gacgcgctgc tgctggccgc
     1321 cggaaagctg cgccggccgc cggccgccga aacctgacag ctgaaccatg tccagcgccc
     1381 ggtcgacccg cgctccgatc tccgcgaccg gcacgccgcg aaccgacaac ggaaagccga
     1441 tgttgtccgc gacgctcata tgcggaaaca gcgcatagtt ctggaacacc atgccgatat
     1501 tgcgctggtg cggcggcata tgggacagcg acttgccttc gagcaggatc tgccctccgg
     1561 tgggcgtctc gaaacctgcg agcagcatca gcgtcgtcgt cttgcccgag cccgacgggc
     1621 ctagcaaagt caggaactcg cctcgttcga tatccagatc gagatcgtcg acgacgcggg
     1681 tgatgccatc gtagcttttt tcgacgccga cgaagcggac gatgggttcg gacgggttca
     1741 tgcgcgtggc agtgaggggg caatcatcgt cccttgccgg ccatccggtc aacgcgggcc
     1801 ggtaggccgg cagcgtcgtc aggcgagacc cttttccttc accatcgccc aggtatcgtc
     1861 gagcgccttg ccgaagcacg ccatcatgtc gtcgacctcc gcttcgctga tgatcagcgg
     1921 cgggctgaat gccacgatgt cgccgggcat gttgcgaaag atcgcgccgt ggtgctgggc
     1981 gcgacggacc agatgcgcgg cgacgcctgc cttcggcgcg aagctctcgc gggtcgcctt
     2041 gtcacgcacc agctcgacgg cgccgatcaa accgatgccg cgcgcgtcac cgatcagcgg
     2101 atgcgcggcg tagctccgga tcgccgcctg cagccgggga ccgaggcgac gtaccgcgcc
     2161 gacgatgtcg cgctcggcat agatcttgag cgtctcgagc gcgacggcag ccggaaccgg
     2221 atgcgacgaa taagtgtaac catggccgaa gatgccgagc ttgtcgcttt gcgcgaggag
     2281 aatgtcgtat aacgcattcg acaccatcgt ggccgagatc ggcaggtagc cggccgaaag
     2341 cgccttggcg acggtgatca tgtcgggctc gagattgaac gtctgcgagc caaacaggtt
     2401 tccggtgcgg ccgaatccgc agatgacttc gtcgacgacg aacagaatgt cgtatttctt
     2461 caggatcggc tggatgcggt cgaagtaggt cgccggcgga acgatgacgc cgcccgcgcc
     2521 catcaccggc tcggcgaaaa acgccgcaac cgtgttggga tcctcgtgaa caatcaacgc
     2581 ctcgagattg gcggccagcc gttccgcgaa cgcttcttcg ctctcgcccg cacgcgcgcc
     2641 gcgataatag tacg
//
LOCUS       DATANS010000378         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_774554, whole genome shotgun sequence.
ACCESSION   DATANS010000378 DATANS010000000
VERSION     DATANS010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774554"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..370
                     /locus_tag="VHT22_05500"
                     /pseudo
     CDS             95..370
                     /locus_tag="VHT22_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992206.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
     gene            721..939
                     /locus_tag="VHT22_05505"
     CDS             721..939
                     /locus_tag="VHT22_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137043.1"
                     /translation="MGPVKILAIILIVAGVLGLAYGTFTYTKEEHTANVGPLTLSVKD
                     KETVNVPLWASVGAIAVGVVLLVSGRNK"
     gene            complement(971..1447)
                     /gene="greA"
                     /locus_tag="VHT22_05510"
     CDS             complement(971..1447)
                     /gene="greA"
                     /locus_tag="VHT22_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX3137044.1"
                     /translation="MSRTPLTRTGAEKLKEELRRLKTVDRPAAIVAIAEARSHGDLSE
                     NAEYDAAKDRQGFLEVRIRDIETMLAQAQIVDPAQIDADGQVVFGATVDLEDHASGDR
                     VCYQIVGDDEADIKAGKISVNSPIARALIGKHAGDTAEVKAPAGLREYEVLAVRYV"
     gene            complement(1464..>1690)
                     /locus_tag="VHT22_05515"
     CDS             complement(1464..>1690)
                     /locus_tag="VHT22_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137045.1"
                     /translation="IVDMLLNDEIALVINTVEEKRSAIQDSYAIRRAALNDQIPTYTT
                     MAGARAAALGIQEMRELVPYSMQALQQRLH"
BASE COUNT          268 a          599 c          544 g          279 t
ORIGIN      
        1 cgcccggtag agaagcgcag tgccgcgtga agccggtcgc agtcgccttg cggcgggcgc
       61 cgcgactcca gcggcgatcg gatagaatgc gccgatggaa acgctgcctc ctgcccgacg
      121 ccgggcgctt cgcgccaagg cgcaccacct gcacccggtg gtgagcatcg gacagcatgg
      181 gctgactccg gcggtgcttc acgagctcga cgtcaatctc ctcgcgcacg aactgatcaa
      241 ggttcgggtg ttcagtgacc tgcgcgatga gcgcgatgcg atgctgcaac gcatctgcgc
      301 tgctctggac gcgacgccgg tccagcacat cggcaagctc ttggtcctgt ggcggcaggg
      361 gcccgagaag ccggcagcga cagtgccgcg gcccaaggaa gcgccgcgcg ctgccggcgc
      421 ccgccgcgct cgcgagccgg ctccgggtga gccgcgcggc aagcggcggc ccgcggcgcg
      481 tccagcccac aagactatgg ccaaatccac cccgcaagcg cgccgcccgc ggacgccgac
      541 tgcgcgcacg accgcgcggc cgcctgccgc cggcgctgtc cggccgcctg ccgcgaagcc
      601 tccaggcgcg cgcgcacggc gccgccgcgg cagcagctga aaaccgcgca cgcgcgttga
      661 gcctagaatg gcggcgtcga ccgcggtcga agccgacgct ttcaacctcc ggaggtaagc
      721 atgggccccg tcaagatcct cgccatcatc ctgatcgttg ccggcgtact cggcctggcg
      781 tacggaacct tcacctatac caaggaagag cacacggcca acgtcgggcc gctgacactc
      841 tcggtcaagg acaaggaaac cgtgaacgtc ccgctctggg ccagcgtcgg cgcgatcgct
      901 gtcggcgtcg tgctgctggt atccggccgc aacaagtaag cagcagcggc gcagcgcttt
      961 gcgcccgccg tcagacgtag cgaacggcaa gcacctcgta ttcgcgcagg cccgcgggcg
     1021 ccttaacctc ggcggtatcg ccggcgtgct tgccgatcag cgcgcgcgcg atcggagaat
     1081 tgaccgatat cttcccggct ttgatgtcgg cctcgtcgtc gccgacgatc tgatagcaga
     1141 cgcgatcacc ggacgcgtgg tcctcgaggt cgacggtcgc gccgaacacg acttggccgt
     1201 cggcatcgat ctgggccggg tcgacgatct gtgcctgcgc aagcatcgtc tcgatatccc
     1261 tgatccgtac ctcgagaaag ccctgccggt ccttggcggc gtcgtactcg gcattttccg
     1321 acaggtcgcc atgcgaccgc gcctcggcaa tcgcaacgat cgccgccggg cggtcgacag
     1381 tcttcagccg gcggagctcc tccttgagct tttccgcgcc ggttctcgtc agtggagtcc
     1441 tgctcatgat gacattctcg tttctagtgc agccgctgct gcagcgcctg catcgagtac
     1501 ggcaccagct cccgcatctc ctggatgccc agcgccgccg cgcgcgcgcc cgccatcgtc
     1561 gtataggtcg gaatctgatc gttgagcgcc gcccgccgta tcgcgtagct atcctggatc
     1621 gcgctgcgtt tttcctcgac cgtattgatc accagcgcga tttcgtcgtt gagcagcatg
     1681 tcgacgatgt
//
LOCUS       DATANS010000379         1913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_228770, whole genome shotgun sequence.
ACCESSION   DATANS010000379 DATANS010000000
VERSION     DATANS010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228770"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1165
                     /locus_tag="VHT22_05520"
     CDS             161..1165
                     /locus_tag="VHT22_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX3137046.1"
                     /translation="MELQTVKFYQTGTFTVGNRLLAEDERSVQANMDRTNSLNSGHRA
                     CQGCGEALGARYAIDAAMRATNGELIAVNATGCLEVFSTPYPETSWKIPWIHSLFGNA
                     AAVASGVAAAMRVKGTPGVRVVAQGGDGGTTDIGFGCLSGMFERNDDVLYICYDNEAY
                     MNTGVQRSSATPPAARTATTPAVGREPGNVFGTGKNVPLIAMAHSIPYVATASVANLR
                     DLERKVAKAMEIAGARYVHIHVPCPLGWGSLPADTIKVARLAVESGLFPLFEAEYGEI
                     TGRTPIRRKVPVTDYLRLQKRFAHLFNVAGEGRVAQIQAIADRNIAKFGLLEEARTHG
                     "
     gene            1158..>1913
                     /locus_tag="VHT22_05525"
     CDS             1158..>1913
                     /locus_tag="VHT22_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3137047.1"
                     /translation="MDKPFAITLDVGSSLANKTGSWRTSRPVYVDRLPPCNHACPAGE
                     NIQSWLYHAEAGDYEAAWRVLTRDNPLPAIMGRVCYHPCETACNRGQLDESVGINSVE
                     RFLGDEALKRGWRFEPPARESGKRVLVVGAGPSGLAAAYHLRLFGHAVTIIEAGPLAG
                     GMMRFGIPKYRLPRDVLDAEVQRILDLGIELRLNRKVSNILETMEQDRFDAAFLAVGA
                     HIAKRAFIPAGSAAHMLDAIAVLRGMEGEDKPLL"
BASE COUNT          345 a          635 c          607 g          326 t
ORIGIN      
        1 ctcgaacagc tgactttcct cgacctcgac gcggacctga tccagttcga gctggagcgc
       61 gaggcgcggg cgcgccgctc gggtccgacc gccgaagcga tccttcgccg ggtgggcacc
      121 gtcgcttcac aggttgccta gcccaccggg agcgttcgcc atggaactgc aaaccgtcaa
      181 gttctaccag accggcacgt tcaccgtcgg caaccggctg ctcgccgagg atgagcgcag
      241 tgtccaggca aacatggatc gcaccaactc gctcaactcc gggcatcgtg cctgccaggg
      301 ttgcggcgag gcgctgggcg cgcgctatgc gatcgatgcg gcgatgcggg cgacgaatgg
      361 cgagcttatc gcagtcaatg cgaccggatg cctcgaggtg ttttccacac cctatcccga
      421 gacatcgtgg aagatcccct ggatccattc gctgttcggc aacgcagcgg ccgtcgcctc
      481 cggcgtcgcg gcggccatgc gggtcaaagg cacgccaggc gtccgtgtcg tcgcccaggg
      541 cggcgacggc gggacgaccg acatcggatt cggctgcctg tcgggaatgt tcgagcgcaa
      601 tgacgacgtt ctctacatct gctacgacaa cgaggcctat atgaacaccg gcgtccagcg
      661 cagcagcgcc acgccgcccg ccgcacgaac cgccaccacg ccggctgtcg gccgcgagcc
      721 gggcaacgtt ttcggcaccg gcaagaacgt cccactgatc gcgatggcgc actcgattcc
      781 gtatgttgcg acggcgagcg tcgccaatct gcgcgacctc gaacgcaagg tcgccaaggc
      841 gatggagatc gccggcgcac gctatgttca catccatgtc ccctgcccgc tcggctgggg
      901 gtcgcttcct gccgatacga tcaaggtcgc tcggctcgcg gtcgaatccg gactgtttcc
      961 tctgttcgaa gccgagtacg gcgaaatcac cggacggaca ccaatccgac gcaaggtgcc
     1021 ggtgaccgat tatttgcgcc tgcagaagcg ctttgcacac ctgttcaacg tcgccggcga
     1081 agggcgcgtc gcgcagattc aggcgatcgc ggaccgcaac atcgcgaaat tcggtctgct
     1141 ggaagaagct cgcacgcatg gataagccat tcgccatcac gcttgacgtc ggctcatcgc
     1201 tcgccaacaa gaccggctca tggcgaacca gccgccctgt gtacgtcgat cggcttccgc
     1261 cgtgcaatca cgcctgtccc gccggcgaaa acatccagag ttggctctac catgcggaag
     1321 ccggcgacta cgaggcggcc tggcgcgtgc tcacgcgcga caatccgctg ccagcgatca
     1381 tggggcgggt ctgctatcac ccatgcgaaa ccgcgtgcaa ccggggtcaa ctcgacgaat
     1441 cggtcggcat caattcggta gagcggtttc taggtgacga ggcgctcaag cgtggctggc
     1501 gcttcgagcc gccggcacgc gaatcgggaa agcgcgtgct ggtcgtcggg gccggacctt
     1561 cgggactcgc ggcggcttac cacctgcggc ttttcggcca cgctgtcacg atcatcgagg
     1621 ccgggccgct cgccggcggc atgatgcgct tcggtattcc gaagtaccga ttgccccgtg
     1681 acgttctcga tgcggaggta cagcggatcc tggatctggg gatcgagttg cggctcaacc
     1741 gcaaggtctc gaacatcctc gagaccatgg agcaggatcg gttcgacgcc gcattcctcg
     1801 ccgtcggcgc ccatatcgcc aagcgcgcct tcataccggc cggcagcgca gcgcacatgc
     1861 tggatgcaat cgcggtgctg cgcgggatgg aaggcgaaga caagccgctg ctt
//
LOCUS       DATANS010000380         1918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_650295, whole genome shotgun sequence.
ACCESSION   DATANS010000380 DATANS010000000
VERSION     DATANS010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650295"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VHT22_05530"
     CDS             <1..531
                     /locus_tag="VHT22_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137048.1"
                     /translation="RKPVSRVLAWLAVPWVALLERSIASAQRAFDYLSEDGTRMFPAA
                     AFEETAADLLRLATRPNSRELTPFETMLAEDIEALVWLRVPQLPSSRAWGDYAVVTKR
                     EYEARLPADRSQAKIIPVPPRLFPPELGDRDLLPLPPSRSALAVVLWTVVSLAGIPWL
                     LWRWRQSQKTGEKLLT"
     gene            559..966
                     /locus_tag="VHT22_05535"
     CDS             559..966
                     /locus_tag="VHT22_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX3137049.1"
                     /translation="METLSVLRALAALAQETRLRVFRLLVEHGPLGLPAGEIGRRLGL
                     PPATLSFHIKELATAGLVSAQQQGRSIRYATDFSAMRSLVAYLSENCCRAALGDDDGQ
                     QTACTSDCSPRPATERPVFTMPKPMVRPKMRTL"
     gene            963..1430
                     /locus_tag="VHT22_05540"
     CDS             963..1430
                     /locus_tag="VHT22_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HEX3137050.1"
                     /translation="MKRFHVHVSVPDLAASIRFYSALFAAEPTLRKDDYAKWMLDDPR
                     VNFAISTRGGSAGVNHLGFQVESSTELDAMEAQLLAADRRLVAEKNADCCYVRSDKYW
                     VTDPAGVAWETFHTLSGVPVVGGADAAPVETARSGCGCGCAPGAVAGAKSCCN"
     gene            1480..>1918
                     /locus_tag="VHT22_05545"
     CDS             1480..>1918
                     /locus_tag="VHT22_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEX3137051.1"
                     /translation="MPALLTDQTFWVNVFFAAEWAVRIAMIVVVPFRRSPEAAKGWLL
                     LIFFEPAVGIALYLLIGRPTPPAWRLQRHVEFMELAKPVYERLGRDPNIFHPQLGPTL
                     DHAVTLAENLGRLPILGGNTAEILADYDGTIDRLIADIDMAHDH"
BASE COUNT          318 a          637 c          599 g          364 t
ORIGIN      
        1 cgcaagcccg tcagccgcgt gctggcctgg ctcgcggtgc cctgggtcgc acttctcgag
       61 cggagcatcg ccagcgcgca gcgagcgttc gattatctga gcgaggatgg cacgcgcatg
      121 tttcccgcgg cggcgttcga agagaccgca gccgatctgt tgcgactggc gacgcgaccg
      181 aattcgcgcg agctcacgcc gttcgaaacg atgctcgccg aagacatcga ggcgctggtc
      241 tggctgcgcg tgccgcagct tccgtcgagc cgcgcctggg gcgactatgc cgtcgtcacc
      301 aagcgcgagt acgaagcgcg gcttcccgcc gatcgcagcc aggccaagat cattcctgtg
      361 cccccccgtc tctttccgcc ggaactgggc gaccgcgatc tccttccact gccgccgagc
      421 cgcagcgcct tggcggtggt cctgtggacc gtcgtttcgc tggccggcat accctggttg
      481 ctctggcgct ggcggcaaag tcagaaaaca ggagagaagc tgttgacata gatcctttat
      541 ttccattatt ctggaaatat ggaaacatta tccgtgctgc gtgccttggc tgcgttggcc
      601 caggaaaccc gcctacgcgt attccgcttg ctggtcgagc acggacctct gggccttccc
      661 gcgggcgaga tcggccgccg gctcggcttg ccgcccgcaa ccctgtcgtt ccacatcaag
      721 gagttggcca cggcgggact ggtttcggcg cagcagcagg gtcggtctat ccgttacgcg
      781 actgactttt ccgcgatgcg cagcctggtc gcctacctca gcgagaactg ttgccgcgcc
      841 gcccttggcg acgacgacgg tcagcagacc gcctgcacct ccgattgctc gccgcggccc
      901 gcgaccgagc gaccggtgtt caccatgccc aagccgatgg tacgacccaa aatgagaacc
      961 ctatgaaacg atttcatgtc cacgtgtcgg taccggatct cgcagcgagc attcggttct
     1021 attccgcgtt gttcgccgcc gagcccacgc tgcgcaagga cgattacgcc aaatggatgc
     1081 tggacgatcc acgcgtcaac tttgccatat cgacccgcgg cggcagcgcg ggcgtcaatc
     1141 atctcggctt ccaggttgaa agctcgaccg agctcgacgc gatggaggct cagctcctgg
     1201 cagcggatcg gcgcctcgtt gcggaaaaga atgccgactg ctgctatgtg cgctccgaca
     1261 aatactgggt gaccgaccct gccggcgtcg cgtgggaaac ctttcataca ctgagcggcg
     1321 tccctgttgt cggtggcgcg gacgctgcgc cggtcgagac agcgcgaagc ggctgcggct
     1381 gcggctgcgc tccgggcgcc gtcgcgggcg ccaagtcctg ctgcaattga cgaacgcgcg
     1441 ccgatcgagc gcgacgcgat tcgcgacgct atgacggcaa tgcccgcgct tctcaccgac
     1501 cagaccttct gggtcaacgt tttttttgcc gccgaatggg cggtgcgcat tgcgatgatc
     1561 gtcgtggttc cattccggcg ctcgcccgaa gcggccaagg gctggctgct gctgatcttc
     1621 ttcgagccgg cggtcggcat cgcgctgtac ttgctcatcg gccggccgac tccgccggcc
     1681 tggcgcctgc agcgtcatgt cgagttcatg gagctggcca agccagtgta tgagcgactc
     1741 ggccgcgacc cgaacatctt ccatccgcag ctcggcccga cgctcgatca tgcagtgacg
     1801 ttggccgaaa atctcggacg gcttccgata ctgggcggca ataccgcgga aatcctcgcc
     1861 gactatgatg ggacgatcga ccggctgatc gccgacatcg atatggcgca cgaccacg
//
LOCUS       DATANS010000381         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_565308, whole genome shotgun sequence.
ACCESSION   DATANS010000381 DATANS010000000
VERSION     DATANS010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565308"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /gene="glmU"
                     /locus_tag="VHT22_05550"
     CDS             <1..151
                     /gene="glmU"
                     /locus_tag="VHT22_05550"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166067.1"
                     /note="forms a homotrimer; catalyzes the acetylation of
                     glucosamine-1-phosphate and uridylation of
                     N-acetylglucosamine-1-phosphate to produce UDP-GlcNAc;
                     function in cell wall synthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX3137052.1"
                     /translation="QLVAPVTVHAGATIGAGATITKDVPPGELTLTEKKQVVRPGWQR
                     PVKKR"
     gene            188..>1783
                     /gene="glmS"
                     /locus_tag="VHT22_05555"
     CDS             188..>1783
                     /gene="glmS"
                     /locus_tag="VHT22_05555"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX3137053.1"
                     /translation="MCGIIGAVAARNVVPVLLEGLKRLEYRGYDSCGVAVNNGGLKRA
                     RSVRRVQDLIAQVEETRLEGTTGISHTRWATHGAPVTMNAHPHFSRDSIALVHNGIIE
                     NYEELRRELQAAGFVFESQTDTEVITHLVNHLYTGDLLKAVQQAIKRLRGAYAIAVFC
                     RDEPQRVVAARFGSPLVVGLGDHENFVASDAMALAGTTDRIIYLEEGDVADIQQDAVR
                     IYDRTDAPVEREVRIIKALAGAAELGPYRHFMQKEIFEQPRAISDTLEGVDAVTPLLF
                     GERAEQIFGRIDSLLILACGTSYYAGLTAKYWLEEIAKLPTQVEIASEYRYRESVPNP
                     NSLVVVISQSGETADTLAALKHAQSLGMTETLAICNVASSAIVRLTKLNYLTRAGVEI
                     GVASTKAFTTQLAALFMLTLALAKLRGRLSAEAEAAALRNLRHLPVALQAVLALEPQI
                     IAWAGQFARKEHALFLGRGRHFPIALEGALKLKEISYIHAEAYAAGELKHGPLALVTD
                     DMPVVTVAPNDALLEKLESNLHEV"
BASE COUNT          347 a          571 c          566 g          299 t
ORIGIN      
        1 ccaattggtt gcgccggtca ccgtccatgc gggcgcaacc ataggtgccg gcgcgaccat
       61 taccaaggac gtgccgccag gcgaactcac gctgaccgag aaaaagcagg tcgtgcggcc
      121 gggctggcaa cggcccgtca aaaagcggta gccgccgcca tcgtcttgaa gaaagggtaa
      181 ccagcaaatg tgcggtatca tcggcgcggt cgctgcgcgc aacgtagtgc ccgttctgct
      241 cgagggcttg aaacggctgg agtaccgcgg ctacgattcg tgcggcgtag ccgtgaataa
      301 cggcggcctc aagcgcgcgc gctcggtgcg ccgtgtgcag gacttgatcg ctcaggtcga
      361 agaaactcgc ctggaaggca cgaccgggat ctcgcatacg cgctgggcca cgcatggcgc
      421 gccggtcacg atgaacgcgc acccgcattt ctcgcgcgac agcattgcgc tggtgcataa
      481 cggcatcatc gaaaattacg aagagctccg gcgcgagctg caggcggccg gcttcgtgtt
      541 cgaaagccag accgacaccg aagtcatcac ccacttagtc aaccatctgt acacaggtga
      601 cctgctcaaa gcggtgcagc aggcgatcaa gcggctgcgc ggcgcctacg caatcgcggt
      661 gttctgccgc gacgagccgc agcgcgtggt ggccgcgcgc tttggcagcc cgctcgtggt
      721 cggcttgggc gaccacgaaa acttcgtcgc gtccgatgcg atggcgctcg ccggcaccac
      781 cgaccgcatt atctatctcg aagagggcga cgtcgccgat atccagcagg acgcagtccg
      841 aatctacgac cgcaccgatg cgccggtcga gcgcgaggtg cgcatcatca aggcgctcgc
      901 cggcgcggcc gaactcggcc cctaccgcca cttcatgcaa aaggaaattt tcgagcagcc
      961 gcgcgcgatc tccgacacgc tcgaaggcgt cgatgccgtc acgccgttgc tgttcggcga
     1021 gcgtgcggag cagatattcg gccgcatcga ttcgctgctg atactggcct gcggcacgag
     1081 ctactatgcg ggcctcacgg caaaatactg gctcgaggaa attgcgaagc tgcccaccca
     1141 ggtcgaaatc gcgagcgaat accgttatcg cgaaagcgtg cccaacccga actcgctcgt
     1201 cgtcgtgata tcgcagtccg gcgaaaccgc cgacacgctc gctgctttga agcatgcgca
     1261 gtcgctgggc atgaccgaaa cgctcgcgat ttgcaatgtg gcgtcgagcg cgatcgtgcg
     1321 gttgaccaag ctcaattatc tgacccgcgc cggcgtcgag atcggcgtgg cgtcgaccaa
     1381 ggcgttcacc acccagctcg cggcgctctt catgctgacg ctggcattgg caaaactgcg
     1441 cggacgcttg tccgcggagg cggaagctgc ggccctgcgc aatctgcgtc atttgccggt
     1501 cgccttgcag gcggtgctcg cactcgagcc gcaaatcatt gcgtgggcgg gccagttcgc
     1561 gcgcaaggag cacgcgttgt tcctcggccg cgggcggcat tttccgatcg cgctcgaagg
     1621 cgcgctaaag ctcaaggaaa tttcgtatat ccacgctgaa gcgtacgcgg cgggcgaact
     1681 caagcacggc ccgctcgcgc tggtgaccga tgacatgccc gtggtcaccg tcgcgccgaa
     1741 cgacgcattg ctggaaaagc tcgagagcaa cctgcacgag gtg
//
LOCUS       DATANS010000382         1956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_523827, whole genome shotgun sequence.
ACCESSION   DATANS010000382 DATANS010000000
VERSION     DATANS010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523827"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VHT22_05560"
     CDS             <1..711
                     /locus_tag="VHT22_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX3137054.1"
                     /translation="LEVDVWRGGSDGSGRFQTYVVPRQEAQTILDVVTWIQRKLDPTL
                     AYRFACRVGMCGSCAMTVNGRPRWTCRTHVAKVASGKRLQIAPLANLTIVRDLVVDMR
                     EFFDKWAKAGGQFTPTASRRDDFARVAPESPARREVDAAIECIGCGVCYASCDVVAWN
                     PAYLGPAALNRAWTLQNDVRDGGRVARLAAVAGDAGCHACHTQAACTERCPKQLSPTA
                     AIAGLKRMTARAALKGEL"
     gene            708..1046
                     /locus_tag="VHT22_05565"
     CDS             708..1046
                     /locus_tag="VHT22_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HEX3137055.1"
                     /translation="MSARGETRLWLAQRASAAVLALCVLVHLATMIIAVNGGLTAADI
                     LGRTRGNPAWGAFYAVFVAAVAIHAPIGLRTIGAEWLHWRGRGADLCWLAIGVALFVI
                     GIRAVAAVAL"
     gene            1049..1393
                     /gene="sdhC"
                     /locus_tag="VHT22_05570"
     CDS             1049..1393
                     /gene="sdhC"
                     /locus_tag="VHT22_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX3137056.1"
                     /translation="MGWRNRDHPSYWAFLVHRISGLLLSLFLPLHFWVLGRALAGEAA
                     LDGFLRWTERPLVKASEVALVFLLAAHLAGGLRLLLVEFVGWRRQAQRSWVAVAAGVA
                     LFCALAFALNLY"
     gene            1398..>1956
                     /locus_tag="VHT22_05575"
     CDS             1398..>1956
                     /locus_tag="VHT22_05575"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX3137057.1"
                     /translation="MLRIDPGLVENVAKELYIRALKILPPDIKQGFDALAARETATTA
                     QNVLGTMIRNIAVAEATDNLLCQDTGIPIYNVTIGRGVQFGDGDGAALKAAIRKGCER
                     ATREHPLRSSIVHPLTRKNEHTSCGIGVPVIHIDHGDQVDGLKIEMIPKGSGSENNSW
                     LKMAVPAEGVDAVKTFVIDCVLDAGG"
BASE COUNT          290 a          646 c          686 g          334 t
ORIGIN      
        1 ctcgaggtcg acgtctggcg aggcggcagc gacggcagcg ggcgattcca gacttatgtg
       61 gtgccgcgcc aggaggcgca gacgatcctc gacgtcgtca cctggatcca gcgcaagctc
      121 gacccgacgc tcgcctaccg attcgcctgt cgcgtcggca tgtgcggttc gtgcgcgatg
      181 acggtcaacg ggcgtccccg ctggacctgc cgcacgcatg tcgccaaggt tgccagcggc
      241 aagcgcttgc aaatcgcgcc gcttgccaac ttgaccatcg tgcgcgacct ggtcgtcgat
      301 atgcgcgagt ttttcgacaa atgggcgaag gccggcggac agttcacgcc gaccgcgtct
      361 cgccgcgacg atttcgcgcg cgtcgcgccc gagtcgccgg cgcgtcgcga agtcgatgcg
      421 gcgatcgagt gcatcggctg cggcgtctgc tacgcgtctt gcgacgtagt cgcgtggaat
      481 cccgcgtacc tgggtccggc ggcgctcaac cgggcctgga cactgcagaa cgacgtacgc
      541 gacggcgggc gcgtcgcgcg gctggccgcg gtcgccggcg acgccggttg tcacgcctgc
      601 catacgcagg ccgcgtgcac cgagcgctgt ccgaagcagc tttcgccgac cgcggctatc
      661 gccgggctca agcgaatgac ggcgcgcgcc gcgctcaagg gcgagctgtg agcgcgcgcg
      721 gcgaaacgcg tctgtggctg gcgcagcggg cgagcgcggc ggtgctcgcg ctgtgcgtgc
      781 tggtccatct ggcgacgatg attatcgcgg tgaacggcgg cttgaccgcc gccgacattc
      841 ttggccgcac gcgcggcaat ccggcgtggg gtgcttttta cgcggtgttc gtcgcagcgg
      901 tggcgatcca cgcgccgatc ggattgcgca cgatcggtgc ggagtggctg cactggcgcg
      961 gacgcggcgc cgacctctgc tggctggcca tcggcgtcgc gctgttcgtt atcggaatcc
     1021 gcgccgtcgc ggccgtcgcg ctgtgagagt gggttggcgc aaccgcgacc acccgtcgta
     1081 ctgggcgttc ctggtccacc gcatctcggg gcttctgctg tcgctgttcc tgccgctgca
     1141 tttctgggtg ctgggacggg cgctcgccgg cgaggcggcg ctcgacggtt tcctgcgctg
     1201 gaccgaacgg ccgctggtca aggcgtccga agtggcgctc gtcttcctgc ttgcagcgca
     1261 tctcgctgga ggcttgcgcc tgctgctggt ggaattcgtc ggctggcgcc gccaggcgca
     1321 gcggagctgg gtcgccgtcg ccgcgggcgt ggcgctgttc tgtgcgctgg cattcgcgct
     1381 taacctatac tgagagcatg cttcgcatcg atccgggcct cgtcgagaac gtcgccaagg
     1441 aactctacat ccgggcgttg aagatcctgc cgcccgacat caagcaaggc ttcgacgcac
     1501 tggccgcgcg cgagaccgcg accacggcgc aaaacgtgct gggcacgatg attcgcaaca
     1561 tcgccgtcgc cgaggcgacc gacaacctgc tgtgccagga caccggcatt ccgatctaca
     1621 acgtgacgat cgggcgcggc gtccaattcg gcgacggcga cggcgcggcg ctgaaagccg
     1681 cgattcgcaa aggctgcgag cgcgcgaccc gcgagcatcc gctgcgctct tcgatcgtgc
     1741 atccgttgac gcgcaagaac gagcacacct cctgcggcat cggcgtgccg gtgatccaca
     1801 tcgaccacgg cgaccaggtg gacggactga aaatcgaaat gatccccaag ggcagcggct
     1861 cggagaacaa ttcctggctc aagatggcgg tgccggccga aggtgtcgac gcggtcaaga
     1921 ccttcgtgat cgactgcgtg ctcgatgccg gcggca
//
LOCUS       DATANS010000383         3057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_228944, whole genome shotgun sequence.
ACCESSION   DATANS010000383 DATANS010000000
VERSION     DATANS010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228944"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="VHT22_05580"
     CDS             complement(<1..1214)
                     /locus_tag="VHT22_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137058.1"
                     /translation="MTLALSLPPIDERPAHPPETRPAQVQAWIDETMKRESIEAAGVI
                     GDALAATNRAAISDSRRLELAEKFYAAAITLWPILERNFARAAHPLTGNALEAAKVAL
                     TLSAELATAYKRLLAHEADKRILLGGNRLLVALIHRCLQCTTRVLTNSYMSYAPVPAR
                     TWHDAHAIYVHSRSRGLHQTPISSDNPEATPERIYVQALLLALANPYGFLPTQLSHVI
                     QYLQEHSHWAKLTDVAPVHRLAKAVAIVPIGHDFPPFSSNKGGNLEGNKLFLLTFDLA
                     FQLQEQLRTLDTGGKAPAGFPADTAAVAQYMTLLRRLLRQWAIPPARQFNRLPSRARV
                     VMCAGLNAVWQYSPGAHLDIFHPPPGLPTMTACQVINHTPAGYALRQIDSVHAPLRVG
                     DLIALRVEGRAAL"
     gene            complement(1283..2137)
                     /locus_tag="VHT22_05585"
     CDS             complement(1283..2137)
                     /locus_tag="VHT22_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3137059.1"
                     /translation="MPPQQERHFASLSPHGFHRVAYRQWGDPDNPRVVVCVHGLTRNG
                     RDFDALAAAIGDRFRVLCPDMPGRGDSEWLADPNDYTFPTYLTTLTALLAHTSVERCA
                     WVGTSMGGLLGMVLAAQRNTPVVRLVVNDVGPVIESPALARIASYVGADPQFESFAAL
                     EAHIRAVSAPFGALSDSQWQTLAQTTARQVRDGSWRLKYDPGISVPFRVQAAQKGDLW
                     PVWDAISCPTLVLRGAESDLLAAETAAAMRVRGPKPRLIEWAGIGHAPMLLDAEQIAP
                     VADFLDAM"
     gene            complement(2286..2912)
                     /locus_tag="VHT22_05590"
     CDS             complement(2286..2912)
                     /locus_tag="VHT22_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX3137060.1"
                     /translation="MQLPLFQLSPAEQQAWLRERFERPAPTSATNLSDGFRLPGREGM
                     TTPAAVLVPLVNRPDGLTVLFTERSADLPDHPGQISFPGGRIEPEDADAVAAALREAE
                     EEIALPRDRVRPLGRLADYETVTGYRVTPVVGWIEPPIVLRPDPVEVASVFEVPLAFL
                     LEPGNQQRHFRMLGELRRDYHAIPYGERYIWGATAAMLLILDRTLRAE"
BASE COUNT          550 a          963 c         1062 g          482 t
ORIGIN      
        1 agcgccgctc ggccttcgac ccgcagcgcg atcaagtcac ccacgcgcag gggggcatgc
       61 accgaatcga tctgacgcag cgcgtagccg gcgggcgtgt gattgatgac ctggcacgcg
      121 gtcatcgtcg gaaggccggg cggcggatgg aagatgtcca gatgcgcacc ggggctgtat
      181 tgccagaccg cattcaagcc ggcgcacatg acgacgcggg cgcgcgacgg caaccggttg
      241 aattgccgcg ccggcggtat cgcccattgc cgcagcagac ggcgcagcaa agtcatgtat
      301 tgcgccaccg ccgcggtatc ggcgggaaat cccgccggcg ccttgccgcc agtatccaga
      361 gtacgcagct gctcctgcag ctggaaggca aggtcgaagg tcagcagaaa cagtttgttg
      421 ccctcgaggt tgccgccttt gttcgatgag aacggcggaa aatcgtgccc gatcggcacg
      481 atggccaccg ctttcgccag ccgatgcacc ggagccacgt cggtcagctt cgcccaatgc
      541 gaatgctcct gcaggtactg gatcacgtga cttagctgcg tcggcaggaa gccgtaagga
      601 ttggccagcg ccagcagcaa ggcctgcaca tagatccgct ccggcgtggc ttcgggattg
      661 tcgctggaga taggcgtctg gtgcaggccc cgcgaacgcg aatggacata gatcgcgtgc
      721 gcatcgtgcc aggttcgcgc cggtaccggc gcgtacgaca tatagctgtt ggtcaggacg
      781 cgggtcgtgc actgcagaca gcggtggatc agcgcgacca gcaagcggtt gccgccgagc
      841 aggatgcgct tgtcggcctc gtgggcgagc agccgcttgt acgcggtggc gagctcggcg
      901 gacagcgtca gcgcgacctt ggcagcttcg agcgcgttgc cggtcagcgg atgcgcggca
      961 cgcgcgaaat tgcgctcgag tatcggccag agcgtgatcg cggccgcgta gaacttctct
     1021 gcgagctcca ggcgacgcga gtccgagatc gcggcccggt tggtggcggc cagcgcatcg
     1081 ccgatcacgc cggcggcttc gatcgattcc cgcttcatcg tttcgtcgat ccacgcctgg
     1141 acctgcgccg gccgcgtttc gggcgggtgc gcggggcgtt cgtcgattgg cgggaggctg
     1201 agggcgaggg tcaagagcgt ggttggtgaa ggggttacca gtcgattgta gacgatgcgg
     1261 cgtgcggatc gcggcaaagg ccctacatgg cgtcgagaaa gtccgcgact ggtgcgatct
     1321 gctcggcgtc gagcaacatc ggcgcgtgtc cgattccggc ccattcgatc agccggggtt
     1381 tggggccgcg cacgcgcatc gccgcggcgg tctccgccgc gagcagatcc gactccgcgc
     1441 cacgcaggac cagcgtcggg cacgagatcg cgtcccatac cggccagagg tcgccctttt
     1501 gcgccgcctg gacacggaac ggaacggaga tgccgggatc gtatttgagc cgccagctgc
     1561 cgtcccggac ttgccgcgcc gtcgtctgcg ccagcgtctg ccactgcgaa tcggagagcg
     1621 cgccgaacgg cgcggatacc gcgcggatat gcgcctcgag cgcggcgaac gattcgaatt
     1681 gtggatcggc gccgacgtag gaggcgatcc gcgccagcgc cggcgactcg atcaccgggc
     1741 caacatcgtt gacgaccagc ctcacgacgg gcgtatttcg ttgcgcggca agcaccatgc
     1801 cgagcagacc gcccatcgag gtgccgaccc acgcgcagcg ctcgaccgat gtgtgcgcca
     1861 gcagcgccgt cagcgtcgtc aaataggtgg gaaacgtgta gtcgttcgga tcggccagcc
     1921 attcgctgtc gccgcgaccc ggcatgtcgg gacaaagtac gcggaaacga tcgccgattg
     1981 ccgccgccag agcatcgaag tctcgaccgt tgcgagtcag cccgtggacg cagacgacga
     2041 cacgaggatt gtccggatcg ccccattgcc tgtaggcgac gcgatggaag ccatgcggac
     2101 tgagacttgc aaaatggcgt tcttgctgag ggggcatgcg gatcgttgcg cgcgagggtc
     2161 gatgcgcggc gtcacggcat cgtcgagcaa aacgccgatc gcgtcaaatt ttggcagcat
     2221 gcctagcgaa acggcgcagc gggacatcgg cgcgaagccc gggtcgtcga gtatggccag
     2281 gctcgctact ccgcgcgcag cgtgcgatcg aggatcagca gcatcgccgc ggtcgcaccc
     2341 cagatgtaac gctcgccata gggaatcgcg tggtaatcgc ggcgcagctc tcccagcatg
     2401 cggaaatggc gttgctgatt gccgggctcg agaagaaacg ccagcggcac ctcgaagacg
     2461 gaggcgactt cgaccggatc gggcctcagc acgatcggcg gctcgatcca gcctacgaca
     2521 ggcgtcacgc gatatccggt cacggtctcg tagtcggcca ggcgccccag aggccgtacg
     2581 cgatcgcgcg gcaacgcgat ctcttcctcc gcttcgcgca gcgcggcagc gactgcgtcg
     2641 gcgtcctcgg gctcgatgcg gccgccggga aagcttatct gccccggatg gtcgggcagg
     2701 tcggcgctgc gctcggtaaa cagcactgtc aggccgtcgg gacgattgac caggggcacc
     2761 aacacggccg cgggcgtggt catgccttcg cggcccggca gccggaatcc gtcggaaaga
     2821 ttggtcgccg acgtcggcgc cgggcgctcg aaccgctcgc gcaaccatgc ctgttgttcg
     2881 gcaggcgaca actggaaaag aggtaactgc atcgggcgca agtgacccgg atccgggcgc
     2941 ggcgacaatt gtagcgcgcg ccgcaaactc gccagtacag cgaaatgtca gaccagaagc
     3001 ttgatcgcgg cgatgcccaa ggcaccgata agcagagtgc gaaggacgtg cgtcggt
//
LOCUS       DATANS010000384         1696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_356168, whole genome shotgun sequence.
ACCESSION   DATANS010000384 DATANS010000000
VERSION     DATANS010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356168"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1146)
                     /locus_tag="VHT22_05595"
     CDS             complement(97..1146)
                     /locus_tag="VHT22_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019296386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX3137061.1"
                     /translation="MRELRSAPGRLLVVDDNKVNRLLLTRSLELQGHSVASAENGRVA
                     LEMLRREGFDLLLLDMEMPEMDGFQVLEQLAGDRQLRDLPVIVTSSLEGVENVVRCIE
                     LGAEDYLSKPVNPGLLKARIGTSLEKKRLRDQQKELMRRFATPEVAQDLQTSGFSLGG
                     KLVRASVMFSDIRGFTSLAESQPPEETIELLNTYYELMFDAISGHGGVVTLMMGDGLM
                     SVFGAPVPLVDHGESAVRSAQEMVELVDLFNVERKAAGKPPIKIGIGIASGEMVAGYT
                     GTNKRATYTCIGDTVNLAARLETHTKVAQRVILIDEATRSSLSERIAVEPLGAVPFKG
                     KSNAVEVFAVDPAQR"
     gene            complement(1139..1489)
                     /locus_tag="VHT22_05600"
     CDS             complement(1139..1489)
                     /locus_tag="VHT22_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX3137062.1"
                     /translation="MMSAPAIDAATFEALKETTGAEFVVELVDTFLQEAPTMLEDLRR
                     ALAAKDAEKFRRTAHSLKSNSNTFGALTLGAMARELELTSAAKVSEGSAAPVDALAEE
                     YSRVAAALSALRHA"
     gene            complement(1486..>1696)
                     /locus_tag="VHT22_05605"
     CDS             complement(1486..>1696)
                     /locus_tag="VHT22_05605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3137063.1"
                     /translation="EMDGLEATRRIVAHWPAATRPRIIAMTANAMQGDRDMCLAAGMD
                     DYLTKPIRVERLVEALNQASARKDR"
BASE COUNT          285 a          573 c          521 g          317 t
ORIGIN      
        1 agatcttcgt cgttccggga accgtctgcc agtaacccgg atcggcgttc ggtcgcgact
       61 ggccagccgc tgggcgggat cacttcatgc acagccctaa cgctgtgccg gatcgactgc
      121 aaacacttcc actgcgtttg atttgccctt gaacggtacc gcgccgagcg gctcgaccgc
      181 gatgcgctcg ctcaacgacg agcgggtcgc ttcgtcgatc agaatgacgc gttgggcgac
      241 cttggtgtgc gtctcgaggc gcgcggcgag attgacggta tcgccgatgc acgtgtatgt
      301 cgcgcgcttg ttggtccccg tgtatccggc gaccatctcg ccggaggcga tcccgatgcc
      361 gatcttgatc ggcggctttc cggcggcctt gcgctcgacg ttgaacagat cgaccagctc
      421 gaccatctcc tgggcggacc gcaccgcgct ttcgccatgg tcgacaagcg gcaccggcgc
      481 gccgaacacc gacatcagtc cgtcacccat catcagcgtg accacgccac cgtgaccgct
      541 gatcgcatcg aacatgagct cgtaatacgt attgagcagc tcgatggttt cctcgggggg
      601 ctgcgattcc gccagcgaag taaagccgcg gatatcggaa aacatcaccg atgcacggac
      661 cagcttgccg cccagcgaaa aaccggaagt ctgcagatcc tgggccactt ccggagtagc
      721 gaagcggcgc atcagctcct tttgctggtc gcgcagacgt tttttctcga ggctcgttcc
      781 gatgcgcgcc ttcagcaacc ccgggttgac gggcttggac aggtaatctt cggcgccgag
      841 ctcgatgcag cggacgacgt tctcgacgcc ttcgagcgac gaggtcacga tgaccggcag
      901 gtcgcgcagc tgccgatcgc cggccagttg ctcgagcacc tggaacccgt ccatctcggg
      961 catttccatg tccagcagca acaggtcgaa gccttcgcgc cgcagcatct ccagcgcaac
     1021 gcggccgttc tcggccgagg cgacgctgtg tccctgcaat tccaggctgc gcgtcagcag
     1081 cagccggttg accttgttgt cgtcgaccac cagcaggcgg cccggcgcac tacgcagctc
     1141 acgcatgacg caatgcggac agggcggcgg caacgcgcga gtattcttca gccaacgcat
     1201 ccaccggcgc ggcgctgccc tcgctcacct tcgctgcgct cgtaagctcg agctcccgcg
     1261 ccatcgcgcc cagagtgagc gcgccaaagg tgttgctgtt ggacttgagc gaatgcgcag
     1321 tccggcggaa cttctcggcg tctttcgccg caagcgcgcg gcgcaggtcc tcgagcatgg
     1381 tcggcgcttc ctgcaggaag gtatccacca gctcgacaac gaactcggcc cccgtcgttt
     1441 ccttcagcgc ttcgaaggta gcagcatcga tcgccggcgc actcatcatc gatcctttcg
     1501 cgcagaggcc tggttcaacg cctcgaccag gcgttcgacg cggatcggct tggtcaggta
     1561 atcgtccatg ccggcggcca ggcacatgtc gcggtcgccc tgcatcgcgt tggccgtcat
     1621 cgcgatgatg cgcggccgcg tggctgcggg ccagtgcgca acgatgcgcc gcgtggcttc
     1681 caggccgtcc atctcg
//
LOCUS       DATANS010000385         2879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_19249, whole genome shotgun sequence.
ACCESSION   DATANS010000385 DATANS010000000
VERSION     DATANS010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19249"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1919
                     /locus_tag="VHT22_05610"
     CDS             <1..1919
                     /locus_tag="VHT22_05610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3137064.1"
                     /translation="CSLPRFCENNRWPASRDAEPMVRALELAAVPTYPDLPTGVGFCL
                     YIRRALIDAIGMFDPVFGLGYGEENDLCMRAAAAGYRNVLCEDAFVLHLGGSSFGVKR
                     ADLAERNMRILLDRHPQYLEQVHAYIAADPLRPLRELALSQYRVLNEPVPGVLHVIHG
                     HGGGTEYHVRALIAASGQAFRHYLLIAVGDEWQLEEHASDATRTYDFSRLPAEPWSDF
                     LGGICARFGVDLIHLHNISGCRDGLLQALSRLDTPFGYTVHDLNFACPTITFLNARHR
                     YCGAVTDAAVCTACLAAQPEFAGVDIDAWRAKHRALLARSAFLIAPSTWAASILGRYF
                     PEHEVTVIPHGNATGMSRPDAVRSTLPMADDGRPVVAVLGAIGPDKGARRLERLVELT
                     RERDLPLRWVLIGYLDRGREPFQSADGVFTMHGAYDSRALRDLIEHYRVRLVAYPSAG
                     PETFSFTLSEAWAAGRPAIVPPIGALADRVAETGAGWVFSDDEWNSEARMLDRIVSVL
                     DSSRAAELAEAARRANSAVQPTLDAMTDATIAIYRGVLPPAREARAALRPITASRCLT
                     ALRYVPWRPAQAAAAPAAPAQHATAPAANAARDPLGHVARVALSIRHTRTGRALYRLA
                     PASLLAALKARLPR"
     gene            1916..>2879
                     /locus_tag="VHT22_05615"
     CDS             1916..>2879
                     /locus_tag="VHT22_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137065.1"
                     /translation="MNASGEGEWLVRGQAHQRAGRTVDALLCYREALAVNRYAIQAHF
                     HLGEVLRELGRDGEAFDAWRMVLTLQPRHVPTLLAFGALARRIGKTAESAEAYRRAVT
                     FEPQSRAARHGLALTQLAAGEAEAYAEIQRSIDEGVAFDEPQELERLLLAAPASLRKQ
                     ALIGELAMRENDGVPGGVVAMAAKDAAVRGERERALAWLESAETHAATALPEALRLLA
                     LAAARAAPESPWPARYAARCMALHAAVAPLAWPRRTAGDVLRVAYLITPARPLRIGAV
                     EIDPARYLREVVAAHPRERIAAFVFDVGGPAPPEAAPTRAGMTAL"
BASE COUNT          412 a          970 c          999 g          498 t
ORIGIN      
        1 tctgctcgct gccccgcttt tgcgaaaaca accgctggcc ggcgtcgcgt gacgcggagc
       61 caatggtgcg agcgctcgaa ctggccgccg tacctaccta tcccgatctg ccgacgggcg
      121 tcgggttctg cctttacatc cgccgcgcgc tgatcgatgc gatcggaatg ttcgatccgg
      181 tgttcggcct gggctacggt gaggaaaacg atttatgcat gcgtgccgca gctgccggct
      241 atcgcaacgt gctgtgcgag gatgcattcg tcctgcacct gggcggcagc tcgttcggcg
      301 tcaagcgcgc cgatctggcc gaacgcaaca tgcgaatcct tctcgatcgc catccgcagt
      361 acctcgagca ggtgcacgcc tatatcgccg ccgatccgct gcgtccgttg cgcgagttgg
      421 cgctgtcgca gtaccgggtg ctgaacgagc cggtcccggg ggtgctccat gtgatccatg
      481 gccacggcgg cgggaccgaa taccatgtgc gcgcgctgat cgccgcctcg ggccaggcct
      541 ttcgccatta tctgctgatc gccgtcggcg acgaatggca gctcgaggag cacgcgagcg
      601 atgccacgcg gacctacgac ttcagccgct tgccagcgga gccgtggagc gatttcctgg
      661 gcggcatttg tgcgcgcttt ggcgtcgacc tgatccatct gcacaacatt tccggctgtc
      721 gcgacggtct gctgcaggcg ctctcccgcc tcgacacgcc tttcggctat acggtgcacg
      781 atctcaactt cgcctgcccg acgataacct tcctcaacgc gcggcaccgc tattgcggcg
      841 ccgtgacgga cgctgcggtc tgcaccgcat gccttgccgc gcagcccgag ttcgccggcg
      901 tcgacatcga tgcatggcga gcgaagcatc gggcgctgct ggcgcgttcg gcattcctga
      961 tcgcaccttc cacctgggcg gcgtcgattc tgggcaggta ttttccagag catgaggtca
     1021 cggtgattcc gcacggcaac gccaccggca tgtcgcgccc agacgccgtt cgatcgaccc
     1081 ttccgatggc cgacgacggc cggcccgtcg ttgcggtcct cggggcgatc ggaccggaca
     1141 agggggcacg gcggctcgaa cgtctggtcg aattgactcg tgagcgagat ctgccgttgc
     1201 gttgggtgct gatcggctac ctggatcgcg ggcgcgagcc gttccaatcc gccgacggcg
     1261 tgttcacgat gcatggcgcg tacgattcgc gcgcgttgcg cgacttgatc gagcattacc
     1321 gtgtccggct ggtcgcgtac ccgtcggcgg ggccggagac gttcagcttc acgctgtccg
     1381 aagcctgggc cgccggccgg cctgcgatcg ttccgccgat cggcgcgctc gccgaccgcg
     1441 tcgccgaaac gggcgccggc tgggtgttta gcgacgatga gtggaactcc gaagcacgca
     1501 tgctcgaccg catcgtttct gtgctcgact cgtcgcgcgc agcagagctc gcggaagcgg
     1561 cgaggcgcgc caacagcgcc gtccagccga cgctggacgc gatgaccgat gcgacgattg
     1621 cgatttatcg cggggtgctg ccacccgcgc gggaagcgcg cgccgcgttg cggccgatca
     1681 cggcgtcgcg ctgccttacc gcattgcgct atgtgccgtg gcgtccggcg caggctgccg
     1741 ccgcaccggc agcgcccgcg cagcatgcca cggcgcccgc tgcaaacgcc gcgcgtgatc
     1801 cgctggggca tgtggcgcgc gtcgcgctaa gcatccgaca cacgcgcact ggtcgtgcac
     1861 tgtaccgcct ggcgccggcg tcgctgctcg ccgcgctcaa ggcgcggctg ccgcgatgaa
     1921 cgcttccggt gagggcgagt ggctcgtgcg cgggcaggcg caccagcgcg ccggccggac
     1981 cgtcgatgcg ctgctgtgct atcgcgaggc gctcgcagtc aatcgctacg cgatccaggc
     2041 ccattttcat ctgggtgaag tgttgcgtga actcggtcgc gacggcgagg cattcgacgc
     2101 ttggcgcatg gtgctgacgc tgcagccgcg ccatgtgccg accttactcg ccttcggcgc
     2161 tttggcgcgg aggatcggca agacggccga gtctgccgaa gcctatcggc gcgccgtgac
     2221 attcgagccg caaagccgcg cagcaaggca cgggctcgcg ctgacgcagc ttgccgcagg
     2281 cgaggccgaa gcgtacgcgg aaattcagcg atcgatcgac gaaggcgtcg ctttcgacga
     2341 gccgcaggag ctcgagcggc tgctgctcgc cgccccggct tcgcttcgga agcaggcgct
     2401 gatcggcgaa ctcgcgatgc gcgaaaacga tggcgtgccc ggaggggtgg tggcgatggc
     2461 ggccaaagac gccgcagtgc gcggcgagcg cgagcgggca ttggcatggc tcgaatcggc
     2521 cgagacccac gccgcgacgg cattgccgga ggcactgcgt ctgctggcgc ttgccgccgc
     2581 gcgcgccgcg ccggagtctc catggcccgc gcgctatgcc gcgcggtgca tggcgcttca
     2641 cgcggcggtg gcaccgctcg cgtggccgcg gcgcacggcg ggcgatgtgt tgcgggtggc
     2701 ctatctgatc acgcctgcgc ggccgttacg cattggcgcc gtggaaatcg atccggcgcg
     2761 ctatttgcgg gaagtcgtag cggcacatcc tcgcgaacgg atcgcggcat tcgtgttcga
     2821 tgtcggtggg cccgcgccgc ccgaggcagc gcccacccgc gcggggatga cggcgctgg
//
LOCUS       DATANS010000386         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_314942, whole genome shotgun sequence.
ACCESSION   DATANS010000386 DATANS010000000
VERSION     DATANS010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314942"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VHT22_05620"
     CDS             <1..281
                     /locus_tag="VHT22_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX3137066.1"
                     /translation="LLAAHPSRQLAGLVLQDQGGILRGHQQVTTAHGDGEVTSGSYSP
                     TLSRSIALARLPARVTPGDIVHVDVRGKPLAARVVKPPFVRNGKSLID"
     gene            343..726
                     /gene="gcvH"
                     /locus_tag="VHT22_05625"
     CDS             343..726
                     /gene="gcvH"
                     /locus_tag="VHT22_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX3137067.1"
                     /translation="MNVPADLKYTASHEWVRSEPDGTITTGITDHAQQALGDLVFIEL
                     PQAGRTLKAGEACAVVESVKAASDVYAPVAGVVLAVNDAVVAAPEKVNADAYGNWLYR
                     LKPANPGDAAKLLDAAGYIAAAGES"
     gene            752..>2033
                     /locus_tag="VHT22_05630"
     CDS             752..>2033
                     /locus_tag="VHT22_05630"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433924.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HEX3137068.1"
                     /translation="MNEPGPAHGASSRLSLRELEQHRGFVDRHIGTDDADQAAMLRTL
                     GFETRDALIDAIVPAAIRSRKPLALPAAKGEEEALEELRSLAAKNRVYPTYIGQGYYG
                     TYTPGVILRNVLENPAWYTAYTPYQPEISQGRLEALVNFQTMICDLSAMAIANASMLD
                     EATAAAEAMALCRRVSQQSSTTLYVAADVLPQTIDVVRTRAGPLGIAVTVGTADAAAD
                     ADCFAVMLQYPGADGDVRDYRELTARLHAKGALVIAASDLLALTMLTPPGEWGADVVV
                     GSSQRFGVPMGFGGPHAAFLATRDEFKRSLSGRLVGVSVDANGDPAYRLALQTREQHI
                     RREKATSNICTAQVLLAVIASMYAVYHGPQGLSTIAARVHRLTGILRAGFKQLGLDSV
                     NETFFDTLTIATGARTGAIHELATAAHVNLRRIDS"
BASE COUNT          379 a          705 c          653 g          296 t
ORIGIN      
        1 cgttgctcgc cgcgcatccg tcgcggcagc tcgcgggcct cgtcttgcag gaccaagggg
       61 gcatactgcg cggccaccag caggtaacaa ccgcgcacgg cgacggggaa gtcaccagcg
      121 gcagctattc gccgacgctg agccggtcga tcgcgctggc ccgcttgccc gcccgcgtca
      181 cgccgggtga catcgtgcac gtcgatgtgc gcggcaagcc gctggccgcg cgcgtggtca
      241 agccgccgtt cgtccgcaac ggcaagagcc tgatcgactg agcccaatac cgatgacgca
      301 gcctgcgcat cgattcacca ccctggcatg gagccctatc cgatgaacgt acccgcagac
      361 ctcaagtaca ccgcaagcca cgaatgggtc cgcagcgaac cggacggtac gattacgacc
      421 ggcatcaccg atcacgcgca gcaggcgctg ggcgatctcg ttttcatcga attgcctcaa
      481 gccggcagaa cgctcaaggc aggcgaagcc tgcgcggtcg tcgaatcggt caaggcagcc
      541 tccgacgtct acgcgccggt cgcgggcgtc gtgctcgcgg tcaacgacgc cgtcgtcgcc
      601 gcgccggaaa aggtcaacgc cgacgcctac ggcaactggc tgtaccggct gaagcccgcc
      661 aatcctggcg atgcggccaa gctgctcgac gcagccggct acatcgcggc ggcgggcgaa
      721 tcctgatcga ccggagaacc aagcaatggc tatgaacgaa cccggaccgg cgcacggcgc
      781 gagctcgcgc ttgagcttgc gcgagctcga acagcaccgc ggcttcgtcg accggcatat
      841 cggcaccgac gacgccgacc aggcggcgat gctgcgcacg ctcggcttcg aaacgcgaga
      901 cgcgctgatc gacgcgatcg tcccggcggc gataagaagc cgcaagccgc tcgcgctgcc
      961 tgcggcgaaa ggcgaggagg aagcgctgga ggaattgcgc agtctcgcgg cgaaaaatcg
     1021 cgtctacccg acctacatcg ggcagggcta ctacggcacc tacacaccgg gcgtgatact
     1081 gcgcaacgtc ctcgaaaatc ccgcctggta caccgcgtat acaccctacc aaccggaaat
     1141 ctcgcaggga cgcctcgagg cgctggtcaa tttccagacg atgatctgcg acctttccgc
     1201 gatggcgatc gccaatgcct cgatgctcga cgaagcgact gccgcggcgg aagccatggc
     1261 gctgtgccgg cgcgtgagcc agcaatcgag cacgacgcta tacgtggctg ccgacgtgct
     1321 gccgcagacg atcgacgttg tgcgaacgag ggccgggccg ctgggcatcg ccgtcaccgt
     1381 cggaaccgcc gatgcggcgg cggacgccga ctgcttcgcg gtcatgctgc aatacccagg
     1441 ggcggacggc gacgtgcgcg actatcgcga gttgaccgcc aggctgcatg ccaagggcgc
     1501 gctggtgatc gccgcgtccg acttgctcgc gctgacgatg ctgactccac ccggcgaatg
     1561 gggcgccgac gtcgtcgtcg gctcgagcca gcgcttcggc gtgcctatgg gcttcggcgg
     1621 cccgcatgcg gcgttcctcg ccacgcgcga cgaattcaag cgaagccttt ctggacggct
     1681 ggtcggcgtc agcgtcgacg cgaacggcga cccggcatat cgattagcac tgcagacgcg
     1741 cgagcagcat atccgccggg aaaaagccac gtccaacatc tgcaccgcgc aggtgctgct
     1801 cgccgtgatc gccagcatgt acgcggtcta tcacgggccg cagggcctct cgacgatcgc
     1861 cgcgcgggtg caccgcctga ccggaatcct gcgcgccgga ttcaagcaac tcggtctcga
     1921 cagcgtcaac gagaccttct tcgatacgtt gacgatcgcc accggcgcgc gcaccggcgc
     1981 aattcatgag ctcgccaccg cggcgcacgt caatctgcgc cgcatagact cga
//
LOCUS       DATANS010000387         2257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_61751, whole genome shotgun sequence.
ACCESSION   DATANS010000387 DATANS010000000
VERSION     DATANS010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61751"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2230
                     /gene="leuS"
                     /locus_tag="VHT22_05635"
     CDS             <1..2230
                     /gene="leuS"
                     /locus_tag="VHT22_05635"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX3137069.1"
                     /translation="WNQWLFLRMLEKGIAYKKTGVVNWDPVDQTVLANEQVIDGRGWR
                     SDAPVEKREIPMYYLRITDYADELLDNLDRMDGWPERVRTMQANWIGKSEGVRFAFTH
                     DIKGDDGKPIGDGRMYVFTTRADTIMGVTFCAVAPEHPLADHAAKANPGLSSFIAECK
                     RGTVIEAELATMEKRGMPTGLVVRHPLTGDNVPVWVGNYVLMAYGDGAVMGVPAHDER
                     DFEFAKNYALPIRQVIAVAGETFSTDAWQPWYADKTRGRCTNSGKYDGLDYQHAVEAI
                     SADLEAMGLGEKKVTWRLRDWGISRQRYWGTPIPIVHCEACGDVPVPEADLPVILPED
                     CVPDGTGNPLRTRADFVNCTCPRCGRPAQRETDTMDVFVDSSWYYMRYACPDAATMVD
                     ARNEYWMPMDQYIGGIEHAILHLLYARFWTKVMRDMGLVHFDEPFTRLMTQGMLLNHS
                     YSRRGDKGGIDYFSPEDVEVRRDAEGRIVGATAKSDGLPVDYGGIGTMSKSKKNGIDP
                     EEIIARYGADTARLFVMFAGPPEASALWSNDGVEGAYRFLKRLWAYAQSRREALATAP
                     QTIAWPGAAPALRAVRRELYLQLKQADYDYQRIQYNTVVSAGMKMLKSLEDAPFDDSA
                     AAVELVREGLSLLLRVLNPVVPHITHVLWEDLGYAAIRGDIIDTPWPKVDALALAQDE
                     IELMLQVNGKLRGKLTVPSTADHAAIERAAAASPEVQKHGNGATPKKIVVVPGRLVNV
                     VV"
BASE COUNT          434 a          699 c          757 g          367 t
ORIGIN      
        1 ctggaatcag tggctgttcc tgcggatgct cgagaaaggc atcgcctaca aaaaaaccgg
       61 cgtggtgaac tgggatcccg tggatcagac cgtgctcgcg aacgagcagg tgatcgacgg
      121 ccgcggctgg cgctccgacg cgccggtgga aaagcgcgag attccgatgt actaccttcg
      181 gattaccgac tacgccgacg agctgctcga caacctcgac cggatggacg gctggccgga
      241 gcgcgtgcgc acgatgcagg cgaactggat cggcaagagc gaaggcgtgc gcttcgcgtt
      301 cacgcacgac ataaagggcg acgatggcaa gccgatcggc gacggccgga tgtacgtctt
      361 cacgacgcga gccgacacga tcatgggcgt gaccttctgc gcggtcgcgc ccgagcaccc
      421 gctggcagac catgcggcga aggcgaatcc cggactgagt tctttcatcg ccgaatgcaa
      481 gcgcggaacg gtaatcgaag ccgagctggc gacgatggag aaaaggggca tgccgaccgg
      541 acttgtcgtc cgtcatccgc tgaccggcga caacgttccg gtctgggtcg gcaactatgt
      601 gctgatggct tatggcgacg gcgccgtcat gggcgtgccc gcgcacgacg agcgggattt
      661 cgagttcgcc aaaaactacg cgctgccgat caggcaggtg atagccgtcg ccggtgaaac
      721 gttttcgacc gacgcatggc aaccgtggta tgcggacaag acccgcggcc gctgcaccaa
      781 ctccggcaag tacgacggac tcgactatca gcatgcggtc gaagctattt ccgccgacct
      841 cgaggcgatg gggctgggtg aaaagaaggt gacgtggcgg ctgcgcgact ggggcatctc
      901 gcgccagcgc tactggggca cgccgattcc gatcgtgcac tgcgaggcgt gcggtgacgt
      961 gccggtgccg gaagccgacc ttccggtcat cctgccggaa gactgcgtgc ccgacggcac
     1021 cggcaatcca ctgagaacgc gcgccgattt cgtcaattgc acctgtcccc gatgcggccg
     1081 gccggcgcag cgcgaaaccg acacgatgga cgtgttcgtc gattcgtcct ggtactacat
     1141 gcgctacgcc tgccccgacg cagcgacgat ggtcgacgcg cgcaatgagt actggatgcc
     1201 gatggaccag tacatcggcg gcatcgagca tgcgatcctg cacctgctct acgcacggtt
     1261 ctggaccaag gtgatgcgcg acatggggct cgtccatttc gacgagccgt tcacgcggct
     1321 gatgacgcag ggcatgttgc tcaaccacag ctactcgcgg cgcggcgaca agggcggcat
     1381 cgattatttt tcgcccgaag acgtcgaagt gcggcgcgac gccgagggcc gcattgtcgg
     1441 tgcgacggcg aagtcggacg ggctgccggt cgactacggt ggcatcggca cgatgtcgaa
     1501 aagcaagaag aacggcatcg acccggagga gattatcgca aggtacgggg cggataccgc
     1561 acggctgttc gtgatgttcg cagggccacc cgaggcaagc gcgctgtggt cgaacgacgg
     1621 cgtcgagggc gcctatcggt ttctgaagcg tctgtgggcg tacgcgcagt ctcggcgaga
     1681 ggcgctcgcg acagcaccgc agacgatagc ctggcccggc gccgcgcccg cgctgcgcgc
     1741 ggtgcgccgc gagctctacc tgcagttgaa gcaggctgac tacgactacc agcgcatcca
     1801 gtacaacacg gtcgtgtcgg ccggcatgaa gatgctcaag tctctcgagg acgcgccgtt
     1861 cgacgattcg gccgccgcgg tcgagctcgt ccgcgagggc ctttcgctat tgctgcgcgt
     1921 gctgaatccc gtcgtgccgc atatcacgca cgtgctatgg gaagacctgg ggtacgccgc
     1981 gatccgcggc gacatcatcg atacgccctg gcccaaggtc gacgcgctgg cgctcgcgca
     2041 ggacgaaatc gagctgatgc tgcaggtcaa cggcaagctg cgcggcaagc tgacggtgcc
     2101 ctctaccgcc gaccacgccg cgatcgagcg cgcggccgcc gcgagtcctg aagtgcaaaa
     2161 gcacggcaac ggcgcgacac ccaaaaaaat cgtcgtcgtt ccgggccgcc tggtcaatgt
     2221 cgtcgtctga cttttgcagg aaattgggtc ccggcct
//
LOCUS       DATANS010000388         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_481751, whole genome shotgun sequence.
ACCESSION   DATANS010000388 DATANS010000000
VERSION     DATANS010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481751"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VHT22_05640"
     CDS             <1..560
                     /locus_tag="VHT22_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137070.1"
                     /translation="VWTIVQFLNALSDAADFAGNHPATVMTSPVAAPDFSFELPQRGQ
                     QTIVRPDADEDVLVVLYSLPDSLARLRSLASQRHAFDDRRIRVLALAASATDAQIAVG
                     QIPGGDAIVAIVAPDVAVVYAMFAARNRAAARHAEFLIDRRGRLRAHWLGVPAPGDDR
                     DREIFAAAQELGGGGLPPPPSEHAH"
     gene            611..>1537
                     /locus_tag="VHT22_05645"
     CDS             611..>1537
                     /locus_tag="VHT22_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX3137071.1"
                     /translation="MNASRSTTLAGPAASRAADRRAPQAVAIWLLVCCALVFAIVVVG
                     GVTRLTHSGLSITEWQPIVGTLPPLSAAQWDEAFVKYQATPEYRQVNKGMSLEAFKSI
                     FWWEYAHRLLGRAIGAAFALPLAWFVMRRRLPSGLALPLLAIFALGGLQGALGWYMVQ
                     SGLVDDPRVSQLRLAAHLGLALLIFAAMFWLALSLLRPESEAPPVPSAPVSRRADALQ
                     RFAFLVVALVFVQALSGSLVAGIRAGFAYNTFPMMNGRLVPPEILALLPWYRNFVDNM
                     ATVQFDHRAIAWLLALLIPVFWLFVRSQTAAPR"
BASE COUNT          210 a          524 c          509 g          294 t
ORIGIN      
        1 aggtctggac catcgtgcag ttcctgaatg cgctgtccga cgccgccgat tttgccggca
       61 accacccagc gaccgtcatg acctcgccgg tggccgcgcc cgacttcagc ttcgagctgc
      121 cgcaacgggg ccagcagacc atcgtgcgac cggacgcaga cgaggacgtg ctggtcgttc
      181 tgtattcgtt gccggattcg ctcgcgcgcc tgcgctcgct cgcatcgcag cggcatgcgt
      241 tcgacgaccg gcgcattcgg gtgctggcgt tggcggcaag cgcgaccgac gcacagatag
      301 cggtggggca aattcccggc ggcgacgcga tagtggcaat cgtcgcgccc gacgtcgcag
      361 tcgtttacgc gatgttcgcc gcgcggaatc gcgccgccgc ccgtcacgcc gaattcctga
      421 tcgaccggcg cgggcgcttg cgcgcgcact ggctcggcgt gcctgcgcca ggcgacgatc
      481 gagatcgcga aattttcgcc gcggcgcaag agctcggcgg cggagggctg ccgccaccgc
      541 cgtccgaaca tgcgcactga ccgcagatgc gggccgcggc cccttccggc actcccggta
      601 gaatcgcaac atgaacgcta gtcggtcgac aacgctcgcc ggtcccgccg catcgcgcgc
      661 agccgatcgt cgcgcgccgc aagcggtggc gatctggcta ctggtctgtt gtgcgctggt
      721 cttcgcgatc gtcgtcgtcg gcggcgtgac ccggctcacc cattcgggac tgtcgatcac
      781 cgaatggcag ccgatcgtcg gcacgctgcc tcctctttcg gcggcgcagt gggacgaggc
      841 cttcgtcaaa taccaggcaa cgccggaata ccgtcaggtg aacaagggca tgagcctcga
      901 agcattcaag tcgatattct ggtgggaata cgcgcaccgg ttgctcggcc gcgccatcgg
      961 tgctgcgttt gcgttgcctc tcgcatggtt tgtcatgcgg cgcagattgc cgtcaggact
     1021 tgccctgccg ctgctagcga tcttcgcgct cggcggcctg cagggcgcgc tcggctggta
     1081 catggtgcaa agcgggctgg tcgacgaccc gcgcgtgtcg cagcttcggc tggccgcgca
     1141 cctcggactt gcgctgctga ttttcgcggc gatgttctgg ctggcgctgt cgctgctgcg
     1201 ccctgagtcc gaagcgccgc ccgtgccgag cgccccggta tcgcgccgtg ctgacgcgct
     1261 tcagcggttc gcgttcctgg tcgtcgcgct ggtgttcgtg caggcgctct cgggcagcct
     1321 tgtcgcggga atccgcgccg gcttcgcata caacacattt ccgatgatga acggccggct
     1381 ggtgccaccg gagatcctgg cgcttctgcc ctggtatcgc aatttcgtcg acaacatggc
     1441 gaccgtgcag ttcgatcacc gcgcgatcgc ctggctcctg gcgctgctga tccccgtttt
     1501 ctggcttttc gtgcgttcgc agacggccgc gccgcgc
//
LOCUS       DATANS010000389         6926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_229205, whole genome shotgun sequence.
ACCESSION   DATANS010000389 DATANS010000000
VERSION     DATANS010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229205"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /gene="argA"
                     /locus_tag="VHT22_05650"
     CDS             complement(<1..749)
                     /gene="argA"
                     /locus_tag="VHT22_05650"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEX3137072.1"
                     /translation="MQWIRSAAPYIHAFRGKTFVIAFGGEVVADETFLGIVHDLNLLQ
                     SLGIRLVVVHGSRPQIEAILKQQNIPSKYNRGLRVTDAETLDCVLEGTGHARSRIEAL
                     LSLGVANSPMAGARIRVVGGNFLTAKPIGVLDGVDMQWTGEVRRVDVSAIRQRLDDGD
                     VVLVSPLGYSPTGEIFNLALEEVATQVAVHLSAHKLIFLMETDGVRNGRRQLVSELST
                     HDAEALLGSERKLPADVKHYLPGAIRACENGV"
     gene            976..3138
                     /locus_tag="VHT22_05655"
     CDS             976..3138
                     /locus_tag="VHT22_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX3137073.1"
                     /translation="MASPLLENLNPEQLAAVTLPHVSALILAGAGSGKTRVLTTRIAW
                     LLATGQASPLSILAVTFTNKAAKEMMSRLSALTPVNTRGMWVGTFHGLCNRMLRAHYR
                     DAQLPQAFQIMDMQDQLAMIKRVYKAHNIDDERFPARQLQSLINASKEEGLRPNQVEA
                     HDEFTRRQVEHFALYEQACQRDGVVDFPELLLRTYELLTQHEGLREHYRRRFSHILVD
                     EFQDTNVLQYKWLKQLTSPQTSVFAVGDDDQSIYAFRGANVANMQHFERDFANAAPVK
                     LIKLEQNYRSHGRILDAANALIKHNQARLGKNLWTADAEGEPVRAFAAPSDIDEAAFI
                     VDVVNGLVADGVSPAEIAVLYRSNAQSRVVEHALFNAGMPYRVYGGMRFFERAEVKHA
                     LAYLRLIASPADDGAFLRVINFPPRGIGARSIETLQERAQATGSSLWQTACSQPVAGR
                     AGSSVAAFVGLIESMRNATHGLPLPEAVDHVVHASGLLAHYTGEKDGQERVDNLQELI
                     NAADGFLRETEREIGEGGDDPLMSFLSHAALEAGETQAAEGRPALQLMTVHSAKGLEF
                     HTVFVTGLEEGLFPHENSMSEFDGIEEERRLAYVAITRARRRLYLTHAQSRMLHGQLR
                     YNVASRFLEEFPDGLVQWLSARRRDFTMVEDAAPATRVVAREVPGWRIGQSVKHAKFG
                     LGIIIDAEGRGDDARVQVNFREAGVKWLALEYAKLQPA"
     gene            complement(3148..3963)
                     /locus_tag="VHT22_05660"
     CDS             complement(3148..3963)
                     /locus_tag="VHT22_05660"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HEX3137074.1"
                     /translation="MSDAPIAFELRPAARGLAWLSHAATMFRAAPLPWLLLLFVYYLL
                     VGLAEFGPWGSIGQFVAPLFRPVFAVGFLAAAWSQERGGVPKFAHLFRGFRSDLRALL
                     PLGVVFLGGMLVAVKATVLIDDGQLISLLSGTERPTEAALSSGRVQAAMLFGAACALP
                     TLLATWFAPALVVFHDAGARTALVTSLRASIANWRPIAVYGLAVFAFGGIVPVLALGI
                     AQLLGENAAGPVLLFVITPYMFVLIATLHISDYVGYRDIFPADETGETPAATS"
     gene            complement(3963..4916)
                     /locus_tag="VHT22_05665"
     CDS             complement(3963..4916)
                     /locus_tag="VHT22_05665"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX3137075.1"
                     /translation="MSVYTTVTPEELAGWLTRYAIGAVHEFEPISAGIENTNYFVTAD
                     KGRYVLTLYERLPAEELPFYLNLIAHLASAGVQAPAPEPDRSGALWSILNGKPAGLVA
                     RLEGAAVAQPDVAHCAAAGAVLAQMHVAAGSYRSRLSNRRGPGWWRQAARAVRPFLSA
                     EQNALIASELKFQSGLSKVRLPRGAIHGDFFCDNVLFEGARVSGVIDFGFAATDAYIY
                     DLAIAVNDWCLLADADRKGALDPGRVEAMVAAYTAIRPVTAEERSMWAPLLRAGALRF
                     WLSRLYDLHRPRPGEITSAHDPVDFESILRERIASPPRLPA"
     gene            complement(4982..5332)
                     /locus_tag="VHT22_05670"
     CDS             complement(4982..5332)
                     /locus_tag="VHT22_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HEX3137076.1"
                     /translation="MRLRPVTLFPIIAFVFALAAFAQKPPPANLEPLPEAPPPPPEIA
                     NDPELEAQVTIVRRPTETIEEYRVKGRLTMIKVTPKTGRPYYLVADGADGTFVRRDSL
                     DTGIKVPMWLLFSF"
     gene            complement(5412..6137)
                     /locus_tag="VHT22_05675"
     CDS             complement(5412..6137)
                     /locus_tag="VHT22_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX3137077.1"
                     /translation="MNQTDKIPELIDPEANFATNGQEAWRVLGIMAEFAVATERLRGI
                     RPAVSLFGSARIVPSHAYYQKAERIARLLSDAGFSVISGGGPGLMEAVNRGAHAGRSP
                     AIGLNIVLPHEQAGNAFQDVGINFRYFFARKMMFVRFASAYVVLPGGFGTIDELTECL
                     TLVQTGKTRKIPIILVGGDFWKGLIDWMRDKLVGEGLISPGDMDLMTLIDEPEAIVDA
                     IFDYYESRGFQPTRAERDRMLNL"
     gene            6136..>6926
                     /locus_tag="VHT22_05680"
     CDS             6136..>6926
                     /locus_tag="VHT22_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX3137078.1"
                     /translation="MPTLYLVDGSSYLYRAFHALPDLRTSRGEPTGAIRGVISMLRRL
                     VEDGKPDQFAVVFDAPGKTFRDDWYPQYKATRTAMPDDLAQQIEPLHELIRAYGWPLL
                     MVAGVEADDVIGTLVRRAETEGLDAVISTGDKDLAQLITPRVTLVNTMSNEKLDMQSV
                     VAKFGVRADQILDLLTLTGDAVDNVPGVAKVGPKTAAKWLAEYGTLDQVIAHAGDIGG
                     IVGENLRAALDWLPQGKKLLTVRTDCTLPVAPTGLVVQPVDSAKL"
BASE COUNT         1295 a         2215 c         2273 g         1143 t
ORIGIN      
        1 accccgtttt cgcaggcgcg gatcgcaccg ggcaggtagt gcttgacgtc ggccggcagc
       61 ttgcgttcgc tgccgagcag cgcctcggcg tcatgggtcg aaagctcgct caccagctga
      121 cggcgcccgt tgcgaacgcc gtcggtttcc atcaggaaga tgagcttgtg ggccgaaaga
      181 tggaccgcga cctgggtcgc gacctcttcc agcgcgaggt tgaatatttc cccggtgggc
      241 gaatatccga gcggcgaaac cagcacgaca tcgccgtcgt cgagacgctg tcgaatcgcc
      301 gacacgtcga cgcgacggac ttcgccggtc cattgcatat cgacgccgtc gagcacgccg
      361 atcggcttcg ccgtcaggaa gttgccgccg acgacgcgaa tgcgtgcgcc ggccatcggc
      421 gagttggcga caccgaggga gagcagcgcc tcgatgcggc tccgcgcatg gccggttccc
      481 tccagcacgc agtccagtgt ctcggcgtcg gtcacgcgca gtccgcggtt gtacttgctg
      541 gggatgttct gctgcttgag gatggcctcg atctgcggcc tcgatccgtg cacgacgacc
      601 agacggatgc ccaggctctg cagcaggttg agatcgtgga cgatgcccag gaacgtttcg
      661 tcggcgacga cctctccgcc aaaagcaatg acgaaggtct tgccccgaaa cgcgtggatg
      721 taaggcgcgg ccgagcggat ccattgcacg aatgcccgcg tcgtcgccga gttcgctgcg
      781 ggatttatac gcggaatgcg tggcatcgga tcgaaggtcg catggcggcg ggcgcggccg
      841 attataacgc ccggtggctt gaggcgagag gcgtggcaac gcggtatcgc ctataattgc
      901 gctttacgcg gtgccgacgc gcattgatcg ccacggtccg ccgaccgccg cctgcttgac
      961 ggcgcgcctc ttccgatggc ctcgccttta ctcgagaatc tcaaccccga acagctcgcc
     1021 gcggtcacgc tgccgcatgt gtcggcgctc atcctagcgg gcgccgggag cggcaagacg
     1081 cgcgtgctga cgacgcgcat cgcgtggctg ctcgccaccg gccaggcgag cccgctgtcg
     1141 atccttgcgg tgacgttcac caacaaggcc gccaaggaga tgatgtcccg gctttcggcg
     1201 ctgacgccgg tgaatacgcg cggcatgtgg gtgggcacct tccatggcct ttgcaaccgg
     1261 atgctgcggg cgcattaccg ggacgcacag ctgccccagg cattccagat catggacatg
     1321 caggatcagc tcgcaatgat caagcgggtg tacaaggcgc acaacatcga cgatgagcgc
     1381 tttccggcgc ggcagctgca gagcctgatc aatgcatcga aggaagaggg cctgcgcccg
     1441 aaccaggtcg aggcgcacga cgaattcacg cgacgccagg tcgagcactt cgcgctgtac
     1501 gagcaggcgt gccagcgcga cggtgtcgtc gattttccgg agctgttgct gcgcacctac
     1561 gagctgctga cgcagcacga agggctgcgc gagcattacc ggcgccgctt ttcgcacatt
     1621 ctggtcgacg agttccagga caccaacgtg ctgcagtaca agtggctgaa gcagctgacc
     1681 tcgccgcaga catcggtttt cgcggtagga gacgacgacc agtcgatcta cgcatttcgt
     1741 ggcgcgaacg tcgccaacat gcagcacttc gagcgcgatt tcgctaacgc cgcgccggtc
     1801 aagctgatca agctcgagca gaattaccgg tcgcacggcc gcatcctcga cgcggccaat
     1861 gcgctgatca agcataacca ggcaaggctg ggcaagaatc tatggaccgc cgatgccgaa
     1921 ggcgagccgg tgcgcgcttt tgccgcgcca tccgacatcg acgaggccgc gttcatcgtc
     1981 gacgtcgtca acgggctggt cgccgacggc gtctcgccgg cggaaatcgc agtgctctat
     2041 cgctccaacg cgcagtcgcg tgtggtcgag cacgcattat tcaacgcggg catgccgtac
     2101 cgcgtctacg gcggcatgcg cttcttcgaa cgcgccgaag tgaagcacgc gctcgcctat
     2161 ctgcgcttga tcgcctcgcc cgcggacgac ggcgcctttc tgcgcgtgat caattttccg
     2221 cctcgaggga taggcgcgcg ctcgatcgaa actctgcagg agcgcgcgca ggcaaccggc
     2281 agcagcctct ggcagaccgc gtgcagccag ccggtcgccg gcagggctgg gagcagcgtc
     2341 gccgcgttcg tcgggctgat cgagtcgatg cgaaacgcca cccacggcct gccgctgccg
     2401 gaggcggtcg accatgtcgt gcatgccagc ggacttcttg cgcattacac gggagaaaag
     2461 gacggccagg aacgcgtcga caacctccag gagttgatca atgccgcgga tggtttcctg
     2521 cgcgagaccg agcgcgagat cggcgaaggc ggtgacgatc cgctgatgag ctttctctcg
     2581 cacgctgcgc tcgaggccgg agaaacgcag gccgccgaag gacgtcctgc gctgcagcta
     2641 atgaccgtgc actcggccaa gggcctggag tttcacacag tattcgtcac cggtctcgag
     2701 gaggggctgt ttccgcacga gaacagcatg tccgaattcg acgggatcga ggaagagcgg
     2761 cggctcgcct acgttgcgat cacgcgggcg cggcggcgcc tgtatctgac ccacgcgcaa
     2821 agccggatgc tgcacggcca gcttcgctac aacgtcgcct cgcgcttcct cgaggagttc
     2881 cccgacgggc tcgtgcaatg gctgtcggcc aggcggcgcg acttcacaat ggtcgaggac
     2941 gcggcgccgg cgacgcgagt ggtcgcgcga gaggtgcccg gctggcgcat cggacagagc
     3001 gtcaagcacg ccaagttcgg tctgggcatc atcatcgatg ccgagggccg tggcgacgac
     3061 gcgcgagtgc aggtcaattt ccgcgaagcc ggcgtcaagt ggctcgcgct cgagtatgcc
     3121 aagctgcagc cggcctgact tggcccgcta gcttgttgcc gcgggcgtct ctcctgtctc
     3181 gtcggcgggg aaaatgtcgc gatagccgac gtagtccgag atgtgcaagg tcgcgatcag
     3241 cacgaacata tagggcgtaa tgacgaacaa cagcaccgga cctgcggcgt tctcgcccag
     3301 cagctgcgcg atgcccagcg cgaggacggg cacgatcccg ccgaaagcga acacggcaag
     3361 tccgtagacg gcgatcgggc gccaattggc gatcgaagcc cgcaggctcg tcaccagcgc
     3421 ggtccgggct ccggcgtcgt ggaatacgac cagcgccggc gcgaaccagg tcgcgaggag
     3481 cgtcggcagc gcgcacgccg cgccgaaaag catcgccgcc tgaactcggc cgctgctcaa
     3541 ggccgcttcg gtcggccgct cggtgcccga gagcagcgag atcagctggc cgtcgtcaat
     3601 gagcaccgtg gcctttaccg cgaccagcat gccgccgagg aagacgactc ccaacggaag
     3661 cagcgcgcga agatcgctgc gaaagccgcg gaacaggtgg gcgaacttcg gcacgccccc
     3721 gcgctcctga gaccaggcgg cggccagaaa tccgacggcg aagactggcc gaaacagcgg
     3781 cgcaacgaac tgtccgatcg acccccacgg accgaattcg gccaagccga ccagcagata
     3841 gtagacgaag agcagcagca gccagggcaa aggagcggcg cggaacatcg tcgccgcgtg
     3901 cgagagccac gcgaggccgc gcgccgccgg acgcaattcg aacgcgatcg gcgcgtcgct
     3961 catcaggcgg gcaaccgcgg cggcgacgca atgcgctcgc gcaggatgct ctcgaaatcg
     4021 accggatcgt gcgccgaggt gatctccccc gggcgggggc ggtgcagatc ataaagtcgc
     4081 gacagccaga agcgaagggc acccgcgcgc agcaacggcg cccacatcga ccgctcttct
     4141 gccgtcaccg ggcgaatcgc ggtataggcg gcgaccatcg cttcgacgcg cccggggtcg
     4201 agcgcgcctt tccgatccgc atctgcgagc aagcaccagt cgttgaccgc gatcgccagg
     4261 tcgtagatgt aggcgtcggt cgccgcaaaa ccgaagtcga tcacgcctga cacgcgcgcg
     4321 ccttcgaaca gcacattgtc gcagaagaaa tcgccgtgta tcgcgcctcg tggcagcctc
     4381 accttggaga gaccgctctg gaacttgagc tcggacgcga tcagcgcatt ctgctcagcg
     4441 gagaggaacg ggcggacggc gcgcgcggcc tggcgccacc agccggggcc gcggcgattc
     4501 gacaggcgag agcgatagct gccggccgcc acgtgcatct gcgccagcac cgcgccggca
     4561 gccgcgcaat gcgcgacgtc cggttgcgcg actgcggcgc cttcgagtcg ggcgaccaat
     4621 ccggcgggtt tgccgttgag gatgctccac agcgcgccgc tgcgatccgg ttcgggcgcg
     4681 ggagcctgga cgccggcact cgccagatgc gcgatcagat tcaggtaaaa cggcaattcc
     4741 tccgccggaa gacgctcgta gagcgtcagc acgtagcgac ccttgtccgc agtgacgaag
     4801 taattggtat tttcgatgcc ggccgaaatc ggctcgaatt cgtgcacggc gccaatcgca
     4861 taacgcgtga gccagccggc gagttcttcg ggtgtgacgg tggtatagac cgacatcggg
     4921 catcgccgcg cacttcggcg tgcacgaccg ctttcagcgc tgctaaagga ccgtgcctaa
     4981 atcaaaaaga gaacagcagc cacatcggca ctttgattcc cgtatccaga ctgtcgcgac
     5041 ggacgaacgt gccgtcggcg ccgtccgcaa cgagatagta gggcctgccc gtcttcggag
     5101 tcactttgat catcgtcagg cgtcctttga cgcggtattc ctcgattgtc tcggtcggcc
     5161 ggcgaacgat cgtcacctgc gcctcgagct cgggatcgtt ggcgatttcg ggtggcggcg
     5221 gtggcgcttc gggcagcggc tcgaggttcg ccggtggcgg cttctgcgcg aacgcagcaa
     5281 gcgcgaagac aaaagcgatg atggggaaca gcgttacggg gcgaaggcgc atgttgcgtg
     5341 gcgacgatcg atagttgctc attttagcag gaatcggcgc ctccggtccg gcgcgacgag
     5401 agcgaaagcc gtcagagatt gagcatcctg tcgcgctcgg cgcgtgtggg ttggaatccg
     5461 cggctctcgt agtagtcgaa gatcgcgtcg acgatcgctt cgggttcgtc gatcaaagtc
     5521 atcaggtcca tgtcgccggg agaaatcagg ccttctccga ccagcttgtc gcgcatccag
     5581 tcgatcagac ccttccagaa atccccgccg accagaatga tcggaatctt tcgggtcttc
     5641 ccggtctgca ccagtgtcag acattcggtc agctcgtcga tggtaccgaa gcctcccggc
     5701 aggaccacgt acgcggacgc aaagcgcacg aacatcatct tgcgggcgaa gaaatagcgg
     5761 aagttgatgc cgacatcctg gaatgcattg ccggcctgct cgtgcggcag cacgatgttg
     5821 aggccgatcg ccggcgagcg cccggcgtgc gcgccgcgat tgaccgcttc catcagtccg
     5881 ggtccgccac cggagatcac cgaaaagccc gcgtcggaca acaggcgcgc gatacgctca
     5941 gccttttggt agtaagcatg gctgggcaca atgcgcgcgc tgccgaacag gctgaccgcg
     6001 ggacgaatgc cgcgcagtcg ctcggtcgcg acggcaaact ctgccataat gcccagaaca
     6061 cgccacgctt cctgtccgtt ggtcgcgaaa ttcgcctcag gatcgatcaa ctcgggaatc
     6121 ttgtccgtct gattcatgcc tacgctgtac ctcgtcgacg gctcgtctta tctgtaccgg
     6181 gcgtttcacg ccttgccgga tctgcgcacc tcgcgcggcg agcccacggg cgcgattcgc
     6241 ggtgtcattt ccatgttgcg ccgattggtc gaagacggca agccggacca gttcgctgtc
     6301 gtattcgacg caccgggcaa gacgtttcgc gacgactggt atccgcaata caaggctacc
     6361 cgtacggcga tgcccgacga tctcgcgcag cagatcgagc cgctgcacga gctgattcgc
     6421 gcctacggct ggccgttgct gatggtagcc ggagtcgagg ctgacgatgt catcggcacg
     6481 ctggtgcgcc gcgccgaaac agaaggtctc gacgcggtga tttcgaccgg cgacaaggac
     6541 ctggcccagc tcattacgcc gcgggtgacg ctggtcaaca cgatgagcaa cgagaagctg
     6601 gatatgcaaa gcgtcgttgc caagttcggc gtgcgcgccg accagattct cgatctgctg
     6661 acgctcactg gcgatgctgt cgacaacgtt cccggcgtgg cgaaagtcgg cccgaagacc
     6721 gctgccaagt ggctggccga atacgggacg ctggaccagg tgatcgcgca cgccggcgat
     6781 atcggcggca tcgtcggcga gaatctgcgc gcggcgctcg actggctgcc gcaaggcaag
     6841 aagctcctga cggtccggac cgattgcacg ttgcccgttg caccgaccgg cctagtcgtg
     6901 cagccggtcg attccgccaa actgaa
//
LOCUS       DATANS010000390         2413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_565797, whole genome shotgun sequence.
ACCESSION   DATANS010000390 DATANS010000000
VERSION     DATANS010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565797"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="VHT22_05685"
     CDS             complement(<1..1287)
                     /locus_tag="VHT22_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX3137079.1"
                     /translation="MTKSIWAAAWAVGLCLASGPAAAQFNQFYFFGDSLSDAGSFKPV
                     LPPGTGKFTTNPGPIWAEVLAQRYGSTATPANQGGNDYAEGGARVTQLPGFPATPPTG
                     TATPVAVQVQHFLAKGAVDPRALYSVWAGANDLFVQLGLAQAGAITPTQLQTNLATAA
                     VQLVQQVGILNAAGARYIMVFNLPDVGNTPFGRGSGQGASITALSSFFNSTLSTGLDA
                     LHVDLIRLNVFSLLNEVVANPAAYGFTNASSPACTVPSLVCTSATLVAPNAAQTFVFA
                     DSVHPTTAGHQLIADYAASVLEAPQKIGLLAEAPLQAGQANFRAIDNRMWSSLSAPRA
                     QSKYEAYVVYDYGNYDHSGDFGGGSDHMNTVTIGGDTKLSERLLAGLAFGYSDDKASF
                     GDNGGGFKLNEASLTAYVGYGQGPWYVGATLGAGDLD"
     gene            1274..1627
                     /locus_tag="VHT22_05690"
     CDS             1274..1627
                     /locus_tag="VHT22_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137080.1"
                     /translation="MDFVTVRIEILLGQVVAPREHMHLNPIVRIPVLVAAGALYGPRN
                     KKPGLHCPGWTGGRCTLFGEQGESAADDFDSVALARWRVRSVGERAPGAVVELHIDDV
                     RNGRYRNLSAAPAAA"
     gene            complement(1629..>2413)
                     /locus_tag="VHT22_05695"
     CDS             complement(1629..>2413)
                     /locus_tag="VHT22_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3137081.1"
                     /translation="YLAAKKQKRIASATFMTALVDFTAAGELEVFIDESQVQALEKKM
                     NERGYLEGSEMANSFNMLRSNDLIWSFVINNYLLGRDPFPFDLLHWNCDSTRMPAKMH
                     SFYLRNMYLRNALKDPGGISLADVPIDVSRIVLPTYFVSAIEDHIAPWKTTYAGPRLL
                     GGKSRFVLSGSGHIAGMINPPSANKYGYWTNDKLAPTPDEWLAGAKQHEGSWWSDWLT
                     WLKPHLGPQVAARALGKSGGKGRLKIIEPAPGSYARLRADAR"
BASE COUNT          411 a          752 c          797 g          453 t
ORIGIN      
        1 atcgagatcg ccggcgccga gcgtcgcgcc gacataccac gggccctgcc cgtagcccac
       61 gtacgcggta agcgatgctt cgttgagctt gaatccgccg ccgttgtcgc cgaacgaggc
      121 cttgtcatcg gagtagccga acgcaaggcc ggcgagcagg cgctcggaca gcttcgtgtc
      181 gccgccgatg gtgaccgtat tcatgtgatc gctgccgcca ccgaagtctc cgctatggtc
      241 atagttgcca tagtcgtaga cgacataggc ttcgtacttg ctctgcgcgc gcggcgcgct
      301 gagcgacgac cacatccggt tgtcgatagc gcggaaattc gcctgcccgg cctgcagcgg
      361 cgcttcggcg agcaggccta ttttctgcgg cgcctcgagc accgacgctg cgtagtcggc
      421 gatcaactga tgcccggcgg tcgtcggatg aacgctgtcg gcaaagacaa acgtctgcgc
      481 ggcgttagga gcgaccagcg tcgctgaggt gcaaacgagc gatgggaccg tgcacgccgg
      541 cgagctggcg ttggtgaagc cgtacgccgc cggattggcg acgacttcat tcaacagcga
      601 aaaaacgttc agccggatca ggtcgacatg aagcgcatcg agcccggtgc tgagcgtgct
      661 gttgaaaaag ctcgacagtg cggtgatcga cgctccctgg ccggagccgc gtccgaacgg
      721 cgtgttgccg acgtcgggaa ggttgaatac catgatgtag cgtgcgccgg cggcgttcag
      781 gatgccgact tgctggacga gttgcaccgc cgcggtggcg aggttggtct ggagctgagt
      841 cggtgtaatc gcgccggctt gcgccagccc gagctggaca aacaggtcgt tggcgccggc
      901 ccacaccgaa tacagcgcgc gcggatcgac cgcgcctttg gcgaggaaat gttgcacctg
      961 cacggccacc ggcgtcgcgg tccctgtcgg cggcgtcgcg ggaaaaccgg ggagctgcgt
     1021 cacgcgggcg cctccctcgg catagtcgtt gcctccctgg ttggcgggag tcgcggtcga
     1081 gccgtaacgc tgcgcgagga cttcggccca tatcgggccg ggattggtcg tgaacttgcc
     1141 ggtgccgggc ggaagcaccg gcttgaacga gccggcgtcg ctcaggctgt cgccaaaaaa
     1201 gtagaactgg ttgaattgcg ccgctgccgg cccgctggcg aggcacagtc cgacggccca
     1261 cgccgcggcc caaatggatt tcgtcaccgt ccgcattgaa attctcctcg gtcaggtcgt
     1321 cgcaccgcgc gagcatatgc atcttaatcc aattgtgcgg attcccgtgc ttgtcgctgc
     1381 cggcgccttg tacggtccgc gcaacaaaaa gcccgggctg cattgcccgg gctggactgg
     1441 cggccgctgc acgctatttg gcgagcaggg cgagtctgcc gccgacgact ttgattcggt
     1501 cgccctcgcg agatggcggg ttcgatccgt gggtgaacga gcgcctggtg ccgttgtcga
     1561 gctgcacatt gacgacgtgc gaaatggccg gtaccggaac ttgagcgccg cacccgccgc
     1621 cgcgtgactc aacgtgcatc ggctcgcaat cgcgcgtagc ttccgggcgc gggttcgatg
     1681 attttcaggc ggcccttacc gccgctcttt ccaagcgcgc gggcggccac ctggggtccc
     1741 agatgcggct tgagccaggt cagccagtca ctccaccagg aaccttcatg ctgcttcgcg
     1801 cccgccagcc attcgtcggg cgtcggcgcg agtttgtcat tagtccaata gccgtacttg
     1861 ttcgcggacg gcggattgat catgccggcg atgtgtccgg accccgacag gacgaagcgc
     1921 gacttgccgc cgaggagccg cgggcccgca taggtcgttt tccagggcgc gatgtgatcc
     1981 tcgatcgccg agacgaaata ggtgggcagc acgatcctgg acacgtcgat cggcacgtcg
     2041 gccaggctga tgccacccgg atccttcagc gcattgcgca aatacatgtt gcgcaggtag
     2101 aagctgtgca tcttcgcggg catgcgcgtc gaatcgcagt tccagtgcag caggtcgaac
     2161 gggaacggat cgcgtccgag cagatagttg ttgatgacga acgaccagat caggtcgttg
     2221 ctgcgcagca tgttgaacga gttggccatc tccgatccct ccagatagcc gcgctcgttc
     2281 atcttctttt ccagcgcctg cacctggctc tcgtcgatga acacctcgag ctcgccggcc
     2341 gcggtgaagt cgacgagcgc cgtcatgaac gtcgcgcttg cgatgcgttt ctgtttcttc
     2401 gccgcgaggt aac
//
LOCUS       DATANS010000391         2367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_482106, whole genome shotgun sequence.
ACCESSION   DATANS010000391 DATANS010000000
VERSION     DATANS010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482106"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VHT22_05700"
     CDS             <1..203
                     /locus_tag="VHT22_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HEX3137082.1"
                     /translation="PIDADKKYKVAGWAPVSEDAKAAGGEAIWEVIERYLRDQKTIKP
                     PTLNLPTLAGVAGNPGIAAKNS"
     gene            complement(222..1424)
                     /gene="tyrS"
                     /locus_tag="VHT22_05705"
     CDS             complement(222..1424)
                     /gene="tyrS"
                     /locus_tag="VHT22_05705"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX3137083.1"
                     /translation="MPIDIPHQLAVFKRGADELIVEAELAAKLERGKPLRIKEGFDPT
                     RPDLHLGHTVQFNKLKQLQDLGHHIVFLIGDFTGMIGDPTGRNVTRPPLSREELEANA
                     KTYTDQAFLILDRDRTEVAFNAKWLSALGADGMIRLAARYTVARMLERDDFAKRYASG
                     QPIAVHEFLYPLAQGYDSVALQADVELGGTDQKFNLLVGRELQRHYGQEPQCILTLPL
                     LEGLDGVNKMSKSLDNYVGITEAPAEMFGKLMSISDELMWRYYELLSFRPLDEIARLR
                     RECADGRNPRDAKVELAQEIVARFHSREAAETALAEFESRFRDHAMPESMPEVTLHSG
                     GKGLALPQLCKQAGLTASTSEAMRLIEQRGLKLDGEVVSDKSLVVAPGTTVVVQAGKR
                     KFARVTIA"
     gene            1484..>2367
                     /locus_tag="VHT22_05710"
     CDS             1484..>2367
                     /locus_tag="VHT22_05710"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HEX3137084.1"
                     /translation="MSKLAILAQRAGAGRIAAVFVMMVIGGVAAFGFVPGMMETDMAT
                     RLVVRDLALPALAPAPIGEGYWREARIERGDTLGSVLARLSITDSAAQQFLRTDQGAR
                     PLYQLMPGKSVRVETDDDGRLISLRYLAQGGALLRVDRRTDGFVAASAPPGYGVRTEV
                     RAGEIRSSLFGAADAVGLPDAVTMQLAEVFSGDIDFYHDLRRGDRFAVVYEMREVDGA
                     PAGAGKLVAAEFVNKGVPYRAFLWRGADGSEAYYTEDGKNLRKAFLRSPVEFTRITSG
                     FSLARFHPFLQTWRAHKGV"
BASE COUNT          379 a          755 c          803 g          430 t
ORIGIN      
        1 agccgattga tgccgacaag aagtacaaag tcgccgggtg ggcgccggtg tccgaagacg
       61 cgaaggccgc gggcggcgaa gcgatctggg aagtgatcga gcgctacctg cgcgaccaga
      121 agacgatcaa gccgcccacg ctgaacctgc cgacgctcgc cggcgtggca ggcaatcccg
      181 gaatcgcggc gaagaattcc tgattgcagc ggcgcgagcc atcaggcgat cgtcacccgc
      241 gcgaactttc gcttcccggc ctggacgacg acggtggtgc cgggtgctac gaccagagac
      301 ttgtcgctga cgacctcgcc gtcgagcttc aggccgcgct gctcgatcag gcgcatcgcc
      361 tcggacgtgc tggccgtcag ccccgcctgc ttgcaaagtt gcggcagcgc caggcccttg
      421 ccaccgctgt gcagtgtgac ttcgggcatg ctctccggca tcgcgtggtc gcggaacctc
      481 gattcgaatt cggcgagcgc cgtttccgcc gcctcgcgcg agtggaatcg cgcgacgatt
      541 tcctgcgcga gctctacctt ggcgtcgcgc ggattgcgcc cgtcggcgca ttcgcgcctg
      601 agccgcgcga tttcgtcgag cggccggaac gacagaagct cgtagtagcg ccacatcaac
      661 tcgtcggaga tcgacatcag cttgccgaac atctccgcgg gcgcctcggt gatgccgacg
      721 tagttgtcga gcgacttcga catcttgttg acgccgtcga gcccttcgag cagcggcagg
      781 gtcaggatgc actgcggctc ctggccatag tggcgctgca gttcgcggcc gacgagcaga
      841 ttgaatttct gatcggtgcc gccgagctcg acgtcggcct gcaacgcgac cgagtcgtag
      901 ccttgcgcca gcggatagag gaattcgtgt acggcgatcg gctgcccgct ggcataacgc
      961 ttggcgaagt cgtcgcgctc gagcatccgc gccaccgtgt atctggccgc aagccggatc
     1021 atgccgtctg cgccgagtgc ggacagccat ttggcgttga acgccacttc ggtgcggtcg
     1081 cggtcgagaa tcaggaacgc ctggtcggtg taggtcttgg cgttggcttc gagctcctcg
     1141 cgcgacaacg gcggccgggt gacgttgcgc cccgtcgggt cgccgatcat gccggtgaag
     1201 tcgccgatca ggaaaacgat gtggtggccg agatcctgca gttgcttgag cttgttgaac
     1261 tgaaccgtgt gacccagatg cagatcggga cgggtcggat caaagccttc cttgatccgc
     1321 agcggcttgc cgcgctcgag cttcgcggcg agctcggctt cgacgatcag ctcgtcggcg
     1381 ccgcgcttga agacggccag ttgatggggg atgtcaatcg gcatggagga ggggtctgct
     1441 acaatccgcc ggtccgcgca caggcttggg aggcggttcg agcgtgagca agctcgcgat
     1501 tctagcgcaa agggcgggtg caggccgcat cgcggcggtg ttcgtcatga tggtcatcgg
     1561 cggcgttgcc gcgttcgggt tcgtccccgg aatgatggag acggacatgg cgacgcggct
     1621 ggtcgtccgc gatctggcgc ttccggcgct ggcgcccgcg ccgatcggcg aaggctactg
     1681 gcgcgaagcg cgcatcgagc gaggcgacac gctcggcagc gtgcttgccc gcctgtcgat
     1741 caccgatagc gccgcgcagc agttcctgcg caccgaccaa ggtgcgcgcc cactctacca
     1801 attgatgccc ggcaagtcgg tccgggtcga gaccgatgac gacgggcggc tgatctcatt
     1861 gcgctacctg gcgcaaggcg gcgcgctgct tcgcgtcgac cgccgtaccg acggcttcgt
     1921 cgccgcaagc gcgccgcctg gttacggcgt gcgcactgaa gttcgcgccg gcgaaatccg
     1981 ctcgtcgctg ttcggtgctg ccgacgccgt cggactgccc gatgcggtga cgatgcagct
     2041 ggccgaagtc ttttccggcg atatcgattt ctatcacgac ctgcggcgcg gcgatcgctt
     2101 cgccgtcgtc tatgaaatgc gcgaggtcga cggcgcgccc gccggtgcag gcaaactggt
     2161 tgccgccgag ttcgtcaaca agggcgtccc ctatcgcgct ttcctctggc ggggcgccga
     2221 cggcagcgaa gcgtactaca cggaggacgg caagaatctg cgcaaggcct tcctgcgctc
     2281 gccggtcgaa ttcacgcgta tcacgtccgg cttttcactg gcacggtttc atccgttcct
     2341 gcaaacctgg cgcgcgcaca agggtgt
//
LOCUS       DATANS010000392         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_189487, whole genome shotgun sequence.
ACCESSION   DATANS010000392 DATANS010000000
VERSION     DATANS010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189487"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..851
                     /locus_tag="VHT22_05715"
     CDS             9..851
                     /locus_tag="VHT22_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX3137085.1"
                     /translation="MPTSDGAGVKLQRSLGQSNWARLDPFLMLDHFSTENPDDYIAGF
                     PAHPHRGFETVTYMLDGHMRHQDHLGNTGDLKAGGVQWMSAGRGIIHSEMPQQEAGRM
                     RGFQLWINLPSKEKMKAAAYRDIQSNEIPVVELASGGRVKVIAGTLDSEGRRTPGPIQ
                     GLSTDPLYFDVELPSGARFRHALPAGHHAFIYPFEGSVAVGAEGAQKILATHHAGILS
                     DGDEVEFSGGPDGGRVILLAGRPLLEPIVQYGPFVMNTREEIEQAVRDYQSGKLTDTA
                     AASA"
     gene            complement(870..>1614)
                     /locus_tag="VHT22_05720"
     CDS             complement(870..>1614)
                     /locus_tag="VHT22_05720"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX3137086.1"
                     /translation="AITAAFIASLLAGVTLGYLYAVEAAAAGGVALFFYGLVTRSLTF
                     DVLRAVLRDTMAVTGALFALLVAATMFTLIVRAFGTDRWVAAMLQQLPGGGIAAVFVV
                     LPILALCALVLDAFELIFVVVPVVMPPLLTVVPAATWVAVLTLLILQASFLLPPFGYA
                     VLMVRSRSPRPVPMRALTRALSPYLVAQFAVLTLILAAPGLVWQRNPTTLSSPAASEP
                     ASGDIGDSAPDPQFDLPPDDAAEKPASKE"
BASE COUNT          321 a          531 c          524 g          238 t
ORIGIN      
        1 tcgaagccat tcccacctcg gacggcgccg gcgtcaagct tcagcgcagc ctcggccaat
       61 cgaactgggc ccgcctcgat ccattcctta tgctcgatca tttctcgacc gaaaatcccg
      121 acgactatat cgccggcttc cccgcgcatc cgcatcgcgg cttcgagacg gtgacctata
      181 tgctcgacgg gcacatgcga caccaggatc acctcggaaa taccggcgac ctaaaagcgg
      241 gcggcgtgca gtggatgagc gcgggccgcg gcatcatcca ttccgaaatg ccacagcagg
      301 aagccggccg catgcgcggt tttcagctct ggatcaatct cccgtccaag gaaaaaatga
      361 aggccgccgc ctaccgcgac atccagtcga acgaaattcc ggtggtcgag ctggccagcg
      421 gcggaagggt caaggtcatc gccggaacgc tggacagcga aggcaggcgc acgcccgggc
      481 ccatccaggg actgtcgacc gatccgctgt acttcgacgt cgagcttccg tcaggtgcga
      541 ggtttcgcca tgcgctgcca gccggccatc atgccttcat ctacccgttc gagggatcgg
      601 tcgctgtcgg cgcggagggc gcgcagaaga ttctcgcgac acaccatgcg gggattctat
      661 cggacggtga cgaggtcgaa ttctcgggtg gacccgatgg cggcagagtc atcctgctcg
      721 cgggccggcc gctgctcgag ccgatcgtgc agtacggacc gttcgtgatg aatacgcgcg
      781 aggaaatcga gcaggcggtg cgcgattacc agagcggcaa gctgaccgat acggcggcgg
      841 ccagcgctta aagcggccgc tcgagtcgct cattccttgc tcgcgggctt ttccgccgcg
      901 tcgtccggcg gcaggtcgaa ttgcgggtcg ggggctgaat cgccgatgtc gccgcttgcg
      961 ggctcgctcg ccgcgggcga cgacagcgtc gtcggattgc gctgccacac gagtcccggc
     1021 gccgccagga tcaacgtcag gacggcaaat tgcgcgacca ggtagggcga tagtgcgcgc
     1081 gtcagcgcgc gcatcggaac gggtcgaggc gatcggctgc gcaccatcag aaccgcatag
     1141 ccgaatggcg gcaacaggaa gctcgcctgc aggatcagca gcgtgagcac cgcgacccag
     1201 gttgcggcag gcaccacggt cagcagcggc ggcatcacga cgggcacgac gacgaagatc
     1261 aattcgaatg cgtcgagcac cagcgcgcac agcgcgagaa tcggcaggac gacgaagacc
     1321 gccgcgatgc cgccgcccgg gagttgctgc agcatcgccg cgacccaccg atcggtgccg
     1381 aaagcgcgca cgatcagcgt gaacatcgtt gccgcgacca gcaatgcgaa cagagcaccg
     1441 gtgaccgcca tggtatcgcg cagcacggcg cgcagcacgt cgaaggtcaa cgatcgtgtt
     1501 accagcccgt agaagaacag cgcgacgccg cccgccgccg ccgcttccac cgcgtagaga
     1561 taacccaatg tgactccggc cagcaggctc gcgatgaacg ccgcggtgat ggcc
//
LOCUS       DATANS010000393         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_693597, whole genome shotgun sequence.
ACCESSION   DATANS010000393 DATANS010000000
VERSION     DATANS010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693597"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VHT22_05725"
     CDS             complement(<1..368)
                     /locus_tag="VHT22_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3137087.1"
                     /translation="MRIVHTEASLGWGGQEIRILTEAAGMIARGHQVEIACPREARIF
                     AEAPRFGVPATALPIGRKRPRGVVALRRFLAARQIDAINAHSSTDAWLAALAGRSLSR
                     PLPLVRTRHISAPVPRDALTR"
     gene            complement(415..>1516)
                     /locus_tag="VHT22_05730"
     CDS             complement(415..>1516)
                     /locus_tag="VHT22_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3137088.1"
                     /translation="QAYERVRAVAGANRLRQSMSKQSSASAASSPFLQFVGACAVGLI
                     VYLALKQSETGGFDAARFTSYVIALLTLLDRLRSMSGVNANIQRGLAAAESIFGLIDE
                     RPEADNGTVEIARLRGELEFEHVSSRYAGADKDALEDVSLTIAPGESVALVGPSGSGK
                     TSLINLIPRFYQPSAGRILLDGHDIDALTLANLRSHLALVSQDVTLFDDTVAANIAYG
                     SMTGASREAIEKAALAAHALDFIRELPDGFETLVGENGLRLSGGQRQRLAIARAILKN
                     APILILDEATSALDSESERHVQAAMDALMRGRTTIVIAHRLSTIERVDRIVVLDSGRV
                     VEQGTHAELLARGGLYAKLHRIQFSAAAPVAG"
BASE COUNT          243 a          495 c          531 g          247 t
ORIGIN      
        1 cgtgtcagtg catcgcgcgg caccggcgcc gagatgtgcc gggtccgcac cagcggcaag
       61 gggcgggata gcgagcggcc ggcgagcgca gcgagccagg catcggtcga gctgtgcgcg
      121 ttgatcgcgt cgatttggcg cgcagcgaga aagcgacgca gcgcgacgac gccgcgcggc
      181 cgcttgcgtc cgatcggcag cgcggtcgcc ggcacgccga agcgcggcgc ctcggcgaag
      241 attcgcgcct cgcgcgggca ggcgatctcg acctgatggc cgcgagcgat cattccggcg
      301 gcttcggtca ggatacggat ctcctgtccg ccccagccga gcgaggcctc ggtatgaacg
      361 atgcgcacgg ctaggactgg gtcgcgcggg tacgcattta gcgtgaacag gcccttaacc
      421 agcgaccggc gccgccgccg aaaactggat ccggtgcagc ttggcgtaaa ggccgccgcg
      481 cgcgagcagc tctgcatgcg tgccctgctc gacgactctg ccggagtcga gcacgacgat
      541 gcggtcgact cgctcgatcg tcgacagacg atgcgcgatg acgatcgtcg tgcggccgcg
      601 catcagcgcg tccatcgccg cctgcacgtg gcgctccgat tcggagtcga gcgcggacgt
      661 cgcctcgtcg agaatcagga tgggcgcgtt tttgagaatg gcgcgtgcga tggcgagtcg
      721 ctgccgctgc ccgccggaca ggcgcaagcc gttctcgccg accagcgttt cgaacccgtc
      781 cggcaattcc cggataaagt ccagcgcgtg cgcggcgagc gccgcttttt cgatggcctc
      841 gcgcgatgcg ccggtcatcg agccgtaggc tatattcgcg gcgacggtat catcgaacaa
      901 ggtcacgtcc tggctgacca gcgccaggtg gctgcgcaga ttggccaggg tcaatgcatc
      961 gatgtcgtga ccgtcgagca gtatccggcc ggcgcttggc tgatagaagc gcggtatcag
     1021 gttgatcagc gaggtcttgc cgctgccgga cggaccgacc agcgccacgc tttcgccggg
     1081 tgcgatcgtc aggctcacgt cctccagggc gtccttgtcc gccccggcgt agcgcgacga
     1141 gacgtgctcg aactcgagct cgccgcgcag gcgcgcgatt tcgacggtgc cattgtcggc
     1201 ctccggtcgt tcgtcgatca ggccgaagat gctttcagcc gcggcgagcc cgcgctggat
     1261 gttggcattg acgcccgaca tcgaccgcag ccggtcgagc agggtcagca aggcgatcac
     1321 gtaggaggtg aagcgcgctg catcgaagcc gccggtttcg ctctgtttca acgcgagata
     1381 gacgatcaac ccgactgcgc acgcgccgac gaattgcaga aacggcgagc tggcggcgct
     1441 tgccgacgat tgcttgctca tcgattggcg cagcctgttc gcgccggcga cggcgcgcac
     1501 gcgctcgtac gcctgg
//
LOCUS       DATANS010000394         2816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_104171, whole genome shotgun sequence.
ACCESSION   DATANS010000394 DATANS010000000
VERSION     DATANS010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104171"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1311
                     /gene="queG"
                     /locus_tag="VHT22_05735"
     CDS             199..1311
                     /gene="queG"
                     /locus_tag="VHT22_05735"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX3137089.1"
                     /translation="MANRSSVATAPDFSALARDIRAWGLELGFGEIGIADTGLEAEEA
                     HLLAWLDAGRHGEMDYMARHGVRRARPAELVPGTLRVITARLDYLAPGARDAAATLAD
                     AGKAYISRYALGRDYHKVLRRKLGRLATRIADSVGDFRYRVFTDSAPVLEVALAAKSG
                     LGWRGKHTLLLTRDAGSFFFLGEIYTDLPLPITPAASAHCGSCSACIAACPTGAIVAP
                     YELDARRCISYLTIELKGSIPETLRPLIGNRVYGCDDCQLACPWNKYAQAASEGDFAV
                     RNGLDDADLVTLFAWTSDEFERRMEGSAIRRIGYASWLRNLAVGLGNANYDAAIVAAL
                     AARRDDPSPLVREHVAWALVQQEEKRTAAGVRQRIN"
     gene            complement(1296..2498)
                     /gene="gspF"
                     /locus_tag="VHT22_05740"
     CDS             complement(1296..2498)
                     /gene="gspF"
                     /locus_tag="VHT22_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HEX3137090.1"
                     /translation="MPSFRYEAVDGAGRAQRGLLDAASARAVRDQLRGDGLFPTAIEP
                     AGAGLSARAATTRLASPLLALLTRQLATLVASGMPLDQALAAVAEQSDHPRAARVLAA
                     IREQVAAGEPLAAALSRFPRSFSDLYRGLVAVGAESGQLGGVLSRLADYLEARQVLRA
                     QFTAALIYPALITVIALVVIATLLLYVVPQIVAVYQQSRQALPFLTRALIGTSDFLRA
                     TGVYWLAAIALLVAGAVLLLRNESWRDRWHGLLLRTPVAGSVLRGLDTARFASTLAIL
                     TGSGAPLLRALETAAAVVWARPMRRAAHAAAGLVREGVSLARALASQRVFPPLLVHLV
                     ANGEQSGQLPAMLDRAAREQELDVERRLTWLAALVQPLLIVFMGAIVLVLVLAVMLPI
                     VSMNQLIR"
     gene            complement(2512..>2816)
                     /locus_tag="VHT22_05745"
     CDS             complement(2512..>2816)
                     /locus_tag="VHT22_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137091.1"
                     /translation="RALVMANIEFSCESDSTVRGAATMNTIRIAPGMRCEVRFDPDFV
                     NESIIVTLRNVDRFEPVVFDFHPDALETPVLDDLVNLMLGKPSQFLLRAPLRGFGR"
BASE COUNT          502 a          955 c          943 g          416 t
ORIGIN      
        1 ccagcgtcgt tttccccgcg ccgagctcgc cggacagagt gacgatcatg ccgccgctca
       61 gcgcgggcgc gagcgccgcg ccggaatgca tcgtggcgtt ggcgtcgggc agatacttgc
      121 gcgacatttg cataatcgtc atcgttgaat gtcgctcgcg acacaatggc gagcccttag
      181 cgaaggtctg aacgcatctt ggccaataga tcgtcggtcg caactgcgcc cgatttcagc
      241 gcactcgcgc gcgacatccg cgcctggggc ctcgaactgg gattcggcga gatcggaatc
      301 gccgacaccg gtctcgaggc cgaggaggcg catctgctcg cttggctcga cgcgggccgc
      361 catggcgaga tggattatat ggcacgccat ggcgtgcgtc gggcgcggcc ggccgagctc
      421 gttcccggta ccctgcgcgt gatcaccgcg cggctcgact atctggcacc gggagcacgc
      481 gacgcagctg cgacgctcgc cgatgccggc aaggcgtata tctcgcgcta cgccctgggc
      541 cgcgactatc acaaggtgct gcggcgcaag ctcggtcgcc tggccacccg cattgccgac
      601 agcgtcggcg attttcgtta tcgtgtgttc accgacagcg cgccggtgct cgaagtcgct
      661 ctcgccgcca agtcgggctt gggctggcgc ggaaagcaca cgctgctgct gacccgcgat
      721 gccggctcgt tcttctttct aggtgagatc tacaccgacc tgccgctgcc gatcaccccc
      781 gcggcgagcg cgcattgcgg cagctgcagc gcgtgcatag ccgcctgccc gaccggagca
      841 atcgtcgcgc cttacgagct cgacgcgcgc cgctgcattt cgtatctgac tatcgagctc
      901 aagggcagca ttcctgagac cttgcggccg ctgatcggca atcgagtcta tggctgcgac
      961 gactgccagc tcgcctgtcc atggaacaag tacgcccaag ccgcgagcga gggcgacttc
     1021 gcggtgcgca acggcctcga cgacgccgac ctggtcacgt tgttcgcctg gacgtcggac
     1081 gagttcgaac ggcgcatgga aggcagtgcg atccgccgca taggctacgc aagctggctg
     1141 cgtaatcttg ccgtgggcct gggcaacgcg aactatgacg cggcgatagt cgcggcattg
     1201 gccgcgcgcc gggatgatcc ttcgccgctg gtgcgcgagc atgtcgcctg ggcgctggtg
     1261 cagcaggagg aaaagcgcac cgccgcggga gtccgtcaac ggatcaactg attcatcgac
     1321 acgatgggaa gcatgaccgc cagcaccagc accagcacga tcgcgcccat gaacacaatc
     1381 agcagcggct gcaccagcgc ggccagccag gtcaggcggc gttcgacatc gagctcctgc
     1441 tcgcgcgcgg cgcggtcgag catcgccggc aactggccgc tctgctcgcc attggctacc
     1501 agatgcacga gcagcggcgg aaacacgcgc tggctcgcca gcgcgcgcgc cagcgacacg
     1561 ccttctcgca ccagtcctgc ggcggcgtgt gccgcccgac gcatcggacg cgcccagacg
     1621 accgccgccg cggtttcgag cgcgcgcaac agcggagcgc cgctgccagt caatattgcc
     1681 aaggtgctgg cgaaacgcgc ggtgtcgagg ccgcgcagca cgctgccggc caccggtgtg
     1741 cgcaaaagca gcccgtgcca gcgatcgcgc caggattcat tccgcaacag caggacggcg
     1801 ccggcaacga gcagcgcgat ggctgcgagc caatagacgc cggtcgcccg gagaaaatcg
     1861 ctggtgccga tcagcgcgcg cgtcagaaac ggcaaggcct ggcggctttg ctgatagacg
     1921 gcgacgatct gcggcacgac atagagcaac agcgtcgcga tgaccacaag cgcgatgacc
     1981 gtgataagcg ccggatagat cagcgcggcg gtgaattgcg cgcgcagcac ctggcgcgcc
     2041 tcgagatagt cggcaagccg gctcaacacg ccgcccagct ggccgctttc ggccccgact
     2101 gcgaccaggc cgcgatagag gtcggaaaag ctccgcggga atcgcgacag cgcggccgcc
     2161 agcggctcac cggccgcgac ctgctcgcga atcgcggcaa gcacgcgcgc tgcgcgcgga
     2221 tgatcggatt gctcggcgac cgccgcgagc gcctgatcga gcggcatgcc ggacgcgacc
     2281 agggtcgcga gctggcgcgt cagcagggcg agcagcggtg acgcaaggcg ggtcgtagcg
     2341 gcgcgcgcgg agagccctgc cccggccggc tcgatcgccg tcgggaacag tccatcgccg
     2401 cgcagctggt cgcgaaccgc gcgcgcactg gcggcatcga gcaatccgcg ttgagcgcga
     2461 ccggcgccgt cgactgcctc gtagcgaaac gacggcaccg tttaggcgcg actagcgccc
     2521 gaagccgcgc aacggcgcgc gcagaagaaa ctggctcggc ttgcccagca tcaagttgac
     2581 caggtcgtcg agcaccggag tctccagcgc atcgggatgg aaatcgaaga ctacgggctc
     2641 gaaccggtcg acattgcgca acgtcacgat gatgctctcg ttgacgaagt cggggtcgaa
     2701 acgaacctcg cagcgcatgc ccggcgcgat gcgaatcgtg ttcatcgtcg ctgcgccgcg
     2761 gaccgtcgaa tccgactcgc agctgaactc gatattggcc atcaccagcg cgcgct
//
LOCUS       DATANS010000395         3054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_146796, whole genome shotgun sequence.
ACCESSION   DATANS010000395 DATANS010000000
VERSION     DATANS010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146796"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..1258)
                     /locus_tag="VHT22_05750"
     CDS             complement(302..1258)
                     /locus_tag="VHT22_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX3137092.1"
                     /translation="MSPIPTGQSALACLRAASIALLAGVVAACAPAVKREPPEPSIAA
                     PATASSSIAPPRPGAYYQDDGPGANPPDNLETIPDALPKLEPLHRFANRPYTVFGHDY
                     VPATRLTSYRERGLASWYGRKFHAQKTAIGEPYDMYAMTAAHPTLPLPSYARVTNLAN
                     GRSVVVRVNDRGPFHPGRIIDLSYAAAYRLGIAQKGSGEVEVESILPGAAPPLLAGPL
                     PPVAASEVPSALALPIGQEAGGYVTQLGAFANYANAQEFVARLANQTGSLGVEPRVRQ
                     DAGLFRVYVGPYSTREEARRAADRLRDALGLDSTVRIEPPAQ"
     gene            1340..1864
                     /locus_tag="VHT22_05755"
     CDS             1340..1864
                     /locus_tag="VHT22_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137093.1"
                     /translation="MKKTALITGIALAGAVAALVLALRAFGKADTPGFFGPGTLLADI
                     NIALELLLVAGLTFGMMLARSGNIEAHRVNQTAWVLVNAVLVALIMIASIRTFKLAHL
                     SDLANSGNLIIVIHAAVGALTLVAGLWLVLQMNDILPARWHIRGWKTLMRATLAGYWT
                     VALLGIATYRVWYA"
     gene            complement(1914..3026)
                     /gene="rodA"
                     /locus_tag="VHT22_05760"
     CDS             complement(1914..3026)
                     /gene="rodA"
                     /locus_tag="VHT22_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX3137094.1"
                     /translation="MEKLGALLGKVWQLIVRRIDSYLMAAALAIAGIGMITLVSASDQ
                     SVGRLTSQALSLGFALVLMWAIANIAPQRLARIALPLYLVAVALLVAVAVGGTLVNGS
                     RRWLSIGIARFQPSELMKIALPLMLAWYFQQRETRIHLLDYALALVLIAVPVVLVRRQ
                     PDLGTALIIGATGFYVLYLAGLSWKVIIGLVAVGAAAAPLIWPHLHDYQQERILTFLD
                     PSRDPLGAGYHSSQASIALGSGGVVGKGWLNGTQTHLDFLPERHTDFIFAVFGEEFGL
                     IGASVLLLLYVLLIGRALAITMNASTMFTRLVSGAITLMFFTYAFVNMAMVSGLLPVV
                     GVPLPLVSFGGTALISLFIGLGILMSVQANRKLVST"
BASE COUNT          561 a         1009 c         1005 g          479 t
ORIGIN      
        1 ccccgagaca cccagcggat agttcgactc gccggcgaag gggacgattg cgatcggaat
       61 cgtcgtcgcg ccgccgccga tgatctcgat cgtcagctgg gcatgggcgc tgaaagccgc
      121 cgcacaacag aacgcgaaca gggtgcgcat caacaatcgg gtcatcgggg tcgagatttt
      181 tggtatgtgg gggaaccgcg gcggcggtga tcgattcagg cactcgcgtc gcccgtcgac
      241 gcaaagcgtc ggggcagcgc aatgattata acgaacgtgt gggcgcaacc gggtcaacgc
      301 atcactgcgc cggcggttca atgcggaccg tcgaatcgag ccccaatgca tcgcgcagcc
      361 ggtcggcggc ccggcgtgcc tcctccctgg tcgaataggg accgacatag acacggaaaa
      421 gtcccgcgtc ctgccggact cttggttcga cgccgagact cccggtttgg ttcgccagcc
      481 gggcgacaaa ttcctgtgca ttcgcatagt tggcgaatgc gccgagctga gtgacatagc
      541 cgccggcttc ctgcccgatc ggcagcgcga gcgccgacgg gacctcgctc gcggcgaccg
      601 gcggcagcgg ccccgccagc aacggcggcg ccgcgccggg cagaatgctt tcgacctcga
      661 cttcgccgct gcccttttgc gcaataccca ggcggtaagc cgccgcgtac gacagatcga
      721 taatgcgccc cgggtgaaac gggccgcgat cgttgacgcg cacgaccacg cttctgccgt
      781 tagccagatt ggtgacgcgc gcgtacgaag gaagcggcag cgtcgggtgc gccgctgtca
      841 tagcgtacat gtcgtagggc tcgccgatgg ccgtcttttg cgcatggaac ttgcggccgt
      901 accacgacgc gagtccgcgc tcgcggtagc tggtcagtct cgttgccgga acgtagtcat
      961 ggccgaatac ggtgtacggc cggttggcga accgatgcag cggttcgagc ttcggcaacg
     1021 catcgggaat cgtttcgagg ttgtccggcg gattggcgcc cggtccatcg tcctgatagt
     1081 aggcgccggg ccgcggcggt gcgatcgacg acgacgctgt cgccggcgcc gcgatcgacg
     1141 gctccggcgg ctcgcgcttg accgcgggcg cgcacgccgc caccacgccg gccagcaacg
     1201 cgatcgacgc ggcgcgcaag caggcgagcg cgctttgacc ggtcggaatc ggcgacatat
     1261 agtcggggat tatacgtggg cgacgaatgc ccccgcgccg agcgcgcggt cgcccaatgt
     1321 tcttgcaccg ggaggtgggg tgaaaaaaac tgcgctcatt accggcatcg cgctggccgg
     1381 tgccgtggcg gcgctggtgc tggccctgcg cgccttcggc aaggcggaca cacccgggtt
     1441 tttcggtccc ggcacgctgc tcgccgacat caacatcgca ctcgaattgc ttctggtcgc
     1501 gggacttacc ttcggcatga tgctggcgcg cagcggcaat atcgaggcgc accgcgtcaa
     1561 ccagaccgcg tgggtactgg tcaatgcggt cctggtcgcg ttgatcatga tcgcctcgat
     1621 ccgcacgttc aagctcgcgc atctgtccga tctcgccaac agcggcaacc tgatcatcgt
     1681 catccacgca gcggtcggcg cgctgacgct ggtcgcgggc ctgtggctgg tgctgcagat
     1741 gaacgacatc ctcccggcgc gctggcacat ccgcggctgg aagacgctga tgcgcgcgac
     1801 gctggccggc tactggacgg tcgcgctgct cggcatcgcg acctatcgcg tctggtacgc
     1861 ctgaaggccc gacaacgagc gctggtgtcc gagcgaattt gccggacagg acactaggtg
     1921 ctgaccagct tgcggttcgc ctgcacgctc atcaggatgc ccagcccgat gaacaggctg
     1981 atcagcgcag tgccgccgaa cgagaccagc ggaagcggca cgccgacgac cggcagcagg
     2041 ccgctgacca tcgccatgtt gacaaaggcg taggtgaaga acatcaaggt gatcgcgccc
     2101 gaaaccagcc gcgtgaacat cgtggacgca ttcatcgtga tcgccagcgc ccggccgatc
     2161 agcagcacat agagcagcag caggaccgag gcgccgatca gtccgaactc ctcgccgaaa
     2221 accgcgaaga tgaagtcggt atgccgctcc ggcaggaaat cgaggtgtgt ctgcgtgccg
     2281 ttcagccagc ccttgccgac caccccgccc gaccccagcg cgatcgacgc ctgcgagctg
     2341 tggtacccgg caccgagcgg atcccggctc ggatcgagaa acgtcagtat gcgttcctgc
     2401 tgatagtcgt gcagatgcgg ccagatcagc ggcgcggcgg cggcgccgac tgccaccagc
     2461 ccgatgatca ccttccatga caggcccgcc agataaagga catagaagcc ggttgcgccg
     2521 atgatcagcg cggtgcccag atcgggttgg cgccgtacca ggacgaccgg caccgcgatc
     2581 aggaccagcg caagcgcgta atcgagcagg tgaatccgcg tctcgcgctg ctggaaatac
     2641 caggcgagca tcaatggcag cgcgatcttc atcagctcgg aaggctggaa gcgcgcgatg
     2701 ccgatgctca gccaccgccg cgagccgttg accagcgttc cgccgaccgc gaccgcgacc
     2761 agcaacgcca cggcgaccag atataacggc agcgcgatgc gcgccagccg ctgcggcgcg
     2821 atgttggcga tcgcccacat cagcaccagc gcgaagccga gcgacagcgc ctggctggtc
     2881 aagcggccaa cgctctggtc ggacgccgat accagcgtga tcatgccgat gccggcgatc
     2941 gccagcgccg cggccatcag atagctgtcg atccggcgca cgatcaactg ccagaccttg
     3001 ccgagcaatg cgccgagttt ctccatcaat cggactcgtc gtcggactcg cgcg
//
LOCUS       DATANS010000396         2625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_606138, whole genome shotgun sequence.
ACCESSION   DATANS010000396 DATANS010000000
VERSION     DATANS010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606138"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VHT22_05765"
     CDS             complement(<1..269)
                     /locus_tag="VHT22_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEX3137095.1"
                     /translation="MNLVDRIKNILLSPRTEWPKIAGETATVQSLYIGYILVLAAIAP
                     LALLIRTGGVAIAAAVAHYAIALAITYLMALIVDTLAPTFNGTKD"
     gene            complement(414..1544)
                     /gene="proB"
                     /locus_tag="VHT22_05770"
     CDS             complement(414..1544)
                     /gene="proB"
                     /locus_tag="VHT22_05770"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX3137096.1"
                     /translation="MSATGTTSIVATARRLVIKVGSTLVTDEGRGIDHAAVGRWAEQI
                     AALSTSGKELVLVSSGAIVEGMKRLGWTRRPVAIHELQAAAAVGQMGLVQAYETAFKR
                     FGLHTAQILLTHEDLSDRRRYLNARSTLLTLLRLGVIPVINENDTVTTDEIRLGDNDT
                     LGALVTNLIEADVLILLTDQSGLYSTDPRTDPGATMLRRARAGDPALEAIAGGAGALG
                     RGGMLTKVLAAKRAARSGATTVIAGGREDGVLTRLAAGEMLGTEIAAEMMTLAARKQW
                     LADHVRLAGRLTLDAGAVRALAREGKSLLPIGVTACAGQFERGELVGCFDADGHELAR
                     GLINYSAAETLRILGKPSAQIEKILGYVDEPELIHRDNLVVL"
     gene            complement(1548..2579)
                     /gene="obgE"
                     /locus_tag="VHT22_05775"
     CDS             complement(1548..2579)
                     /gene="obgE"
                     /locus_tag="VHT22_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX3137097.1"
                     /translation="MKFIDEAKIEVIAGDGGNGAVSFRREKYVPRGGPDGGDGGRGGS
                     IHAIADRNINTLIDYRFARIHRAKRGENGRGADQYGRGAEDIVLRMPVGTVITDAETG
                     ETIADLAAHGQSAQLAKGGQGGLGNLHFKSSTNRAPRQSTPGETGEHRLLRLELKVLA
                     DVGLLGMPNAGKSTLIRAISAARPKVADYPFTTLAPNLGVVRVDENRSFVVADVPGLI
                     EGAAEGAGLGHQFLRHLQRTRLLLHIVDLAPLDGDADPVAGARAIVRELERYDRALYD
                     KPRWLVLNKADLIDADERKARVAEFVKSYRWKGPVFTIAAISGEGCRDLVYAVQRWLE
                     AHPSEALTA"
BASE COUNT          418 a          868 c          866 g          473 t
ORIGIN      
        1 aagtccttcg tgccgttgaa cgtcggcgcg agcgtgtcga cgatgagcgc catcaggtag
       61 gtgatcgcca gtgcgatggc gtaatgggca accgcggccg cgattgcgac gccgccggtg
      121 cgtatgagca gcgccagcgg cgcgatggcg gcgagcacca ggatatatcc gatgtacagc
      181 gattgcaccg tcgccgtctc gccggcgatc ttcggccact cggtccgggg agacagcagg
      241 atgttcttga tgcggtcgac caggttcacg atgccctccc ttgatgtcga tcgaaaatcg
      301 gcgctcgctc gttcaccggc tcaatcggag cggcttcttg tgccgcgagt gtaggggaag
      361 gcgaagccca tgtcgatttt cgaccagcga ccggcgatcg cgcgatgcac gcttcagaga
      421 acgaccagat tgtctcggtg gatcagctcg ggttcgtcga cgtaacctag gatcttctcg
      481 atttgcgccg agggcttgcc caggatgcgc agcgtttccg ccgcgctgta attgatcagc
      541 cctcgcgcca gctcatggcc gtcggcgtcg aagcagccga ccagctcgcc gcgctcgaac
      601 tggccggcgc aggcggtgac accgatcggg agcagactct tgccttcccg cgcaagcgcg
      661 cgcactgccc cggcgtccag cgtcaaccgg cccgccagcc gaacgtggtc ggcgagccat
      721 tgctttcgcg cggccagcgt catcatttcg gcggcgattt cagtgccgag catttcgcct
      781 gcggcgagcc gggtcagcac gccgtcctcg cgaccgccgg cgatcaccgt cgtcgcgccg
      841 cttctggcgg cccgtttcgc tgccagcacc ttggtcagca tgccgccgcg gccgagcgcc
      901 ccggcaccgc cggcgatcgc ttccagcgcg ggatcgccgg cacgcgcacg gcgcagcatc
      961 gtcgcgccgg gatcggtgcg gggatcggtg ctgtagagcc cggactgatc ggtgagcagg
     1021 atcagcacgt cggcttcaat caggttggtg accagcgcgc cgagcgtgtc attgtcgccg
     1081 aggcggattt catcggtcgt caccgtgtcg ttttcattga tcacggggat gacgcccagc
     1141 cgcagcaagg tgagcagcgt cgagcgcgcg ttcagatagc ggcggcgatc ggaaagatct
     1201 tcatgcgtca gaaggatctg cgccgtatgc aggccgaagc gcttgaaagc cgtttcatac
     1261 gcctgcacca ggcccatctg tccgacggcg gccgcggcct gcaattcgtg gatcgccacc
     1321 ggacgcctgg tccagccgag acgcttcatg ccctcgacga tggcgccgga ggatacgagg
     1381 accagctcct tgccgctcgt cgacagcgcc gcgatctgct cggcccatcg gccgacggcg
     1441 gcgtgatcga tcccgcgacc ttcgtcggtg accagtgtcg atccgacctt gatcaccagc
     1501 cgccgcgcgg tggcaacgat cgatgtcgtg cctgtggcgc tcatggctta agcggtgagc
     1561 gcttcggaag gatgcgcttc cagccagcgc tggacggcat agacaagatc gcggcagcct
     1621 tcgccgctga tcgcggcaat tgtgaacacc ggacctttcc atcggtacga cttgacgaat
     1681 tcggcaacgc gcgctttgcg ttcgtcggcg tcgatcagat cggccttgtt cagcaccagc
     1741 cagcgcggct tgtcgtacag cgcgcggtcg tagcgttcga gctcgcgcac gatcgcacgc
     1801 gcgccggcga ccggatcggc atcgccatcg agcggcgcga gatcgacgat gtgcagcagc
     1861 aggcgcgtgc gttgcaggtg gcgcagaaac tggtggccga gtcccgcgcc ctccgccgca
     1921 ccctcgatca gtccgggaac atcggcgacg acaaagctgc ggttctcgtc cacgcgaacg
     1981 acgcccaggt tcggcgccaa ggtcgtgaac gggtagtcgg cgaccttcgg ccgggcggcc
     2041 gagatcgcgc gtatcagcgt cgacttgccg gcattgggca tgccgagcag cccaacgtcg
     2101 gcaagcacct tgagctcgag ccgcaacagc cggtgctcgc cggtttcgcc gggcgtcgac
     2161 tgccgcggcg cgcgattggt gctggatttg aagtgcaggt tgcccaggcc gccctggccg
     2221 cctttggcga gctgcgccga ctgaccgtgc gccgcgagat cggcgatcgt ttcgcctgtc
     2281 tccgcgtcgg tgatgaccgt gccaaccggc atgcgcaaga cgatgtcttc ggcgccgcgg
     2341 ccgtactgat ctgcgccgcg gccgttttca ccgcgcttgg ctcgatgaat gcgcgcgaag
     2401 cgatagtcga tcagcgtgtt gatgttacgg tcggcgatcg catgaatgct cccgccgcgg
     2461 ccgccgtcgc cgccgtccgg gccgccgcgg ggcacatact tttcgcggcg aaacgagacc
     2521 gcaccgttgc ctccatcgcc ggcaatgact tcgatcttcg cttcgtcgat gaatttcatc
     2581 tacgtctcac gcgatcgcag atattcgctc accacgatca ggagg
//
LOCUS       DATANS010000397         2401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_104324, whole genome shotgun sequence.
ACCESSION   DATANS010000397 DATANS010000000
VERSION     DATANS010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104324"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="VHT22_05780"
     CDS             <1..961
                     /locus_tag="VHT22_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3137098.1"
                     /translation="KQLLQSWLPQLLGREGNFEVVVFGILMILLLQRTRDGVWPWLAR
                     LLPGEVAHTVVAAAALPRRARPRDSAPLLTVEHARKEFGGLVAVDDLSFEIRPGEILG
                     LIGPNGAGKSTMFNLISGALSPSRGTITFNGQQIGGRQPFQIAQLGIGRTFQHVRLIA
                     GMSVLDNVALGAYLRGRAGVARAALRLDRREEARVRAEAAAQVARVGLADSLHAPAGS
                     LPLGRQRIVEIARALASDPLLLLLDEPAAGLRFLEKQQLATLLRSLREEGMTILLVEH
                     DMDFVMGLTDRLVVMDFGEKLAEGLPADIQRNPVVLEAYLGAV"
     gene            975..1715
                     /locus_tag="VHT22_05785"
     CDS             975..1715
                     /locus_tag="VHT22_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3137099.1"
                     /translation="MSALLEIVDLHVAYGKVEAVHGVSLTVAEGSIVTVIGPNGAGKT
                     TLLAAIMGLLPSRGDIVYAGARVSRTSVEERVAQRLCLVPERRELFGAMTVADNLELG
                     AFQRHRARDRTLGKTRDEVYQRFPRLAERRTQLAGTLSGGERQMLALGRALMAGPRLL
                     LLDEPSLGLAPLIVKEIFSIIAALKASGVSILLVEQNARAALQIASHGYVLETGTVAI
                     AGPSEAISRDARVAATYLGRTPESTSAS"
     gene            1793..>2401
                     /locus_tag="VHT22_05790"
     CDS             1793..>2401
                     /locus_tag="VHT22_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEX3137100.1"
                     /translation="MTDLVVPPVKPALLEQATRLLLAPAALELRQLSDVLAGIHGHRI
                     DHADLYFQYSRVESWSLEEGIVKSGSFSIDRGVGVRAISGEKTAFAYSDDISLDALTQ
                     AATATRAIGRQGQSAVAALDRIGGARALYSSNDPVTALTDEAKVALLERLERLARARD
                     PRITQVMASLAGEYEAILVARSDGSIAADVRPLVRISLNVIAE"
BASE COUNT          373 a          766 c          829 g          433 t
ORIGIN      
        1 gaagcaattg ctgcaaagct ggctgcccca attgctcgga cgcgagggca atttcgaggt
       61 ggtcgtcttc ggcatactga tgatcctgct gctgcagcgc acgcgggacg gcgtctggcc
      121 gtggctggcg cgattgctgc ctggcgaagt tgcccatacc gttgtcgcgg ccgcggcgtt
      181 accgcgacgc gcccgtccgc gcgactcggc gccgctgctg accgtcgagc acgcacgcaa
      241 ggaattcggc ggcttggtcg cggtcgacga tctctcgttc gagatccggc ccggcgagat
      301 tcttggcctg atcgggccga acggcgcagg caagagcacg atgttcaacc tgatcagtgg
      361 cgcgctgtcg ccaagccgcg ggacaatcac gttcaatggc cagcagatcg gcggccggca
      421 gcctttccag atcgcgcaat tgggtattgg ccgcactttc cagcatgtca ggctgatcgc
      481 gggcatgtcg gtgctcgaca acgtcgcgct cggcgcctac ctgcgcggca gggccggggt
      541 cgctcgcgca gcgctgcggc tggaccggcg cgaggaggcg cgcgtgcgtg ccgaagcagc
      601 ggcgcaggtc gctcgcgtcg gactggccga ttcgctgcat gcgccggccg gctcgctgcc
      661 gctgggtcgg cagcggatcg tcgagatcgc gcgcgcgctc gcctccgacc cgctgctgct
      721 cttgcttgac gagcccgcgg cggggcttcg cttccttgaa aagcagcagc ttgcgacctt
      781 gctgcgcagt ctgcgcgaag agggcatgac catcctgctg gtcgagcatg atatggattt
      841 cgtgatgggg cttaccgacc gcctggtcgt gatggatttt ggtgaaaagc tcgccgaagg
      901 attgccggcc gatatccagc gcaaccccgt ggtgctcgag gcgtatctgg gggccgtttg
      961 actgctcgac tgccatgagc gcattgctcg agatcgtcga cttgcacgtc gcctacggca
     1021 aggtcgaggc ggtccacggc gtttcgctga cggtcgccga aggcagcatc gtcacggtga
     1081 tcggccccaa cggcgccggc aagaccacgc tgctcgcggc gatcatggga ttgctgccgt
     1141 cgcgcgggga tatcgtgtac gccggcgcgc gggtgagccg gaccagcgtc gaagagcgcg
     1201 tggcgcaacg actctgcctg gtcccggaac gccgcgaatt gttcggcgcg atgacggtgg
     1261 cggacaacct ggagctcggc gcgtttcagc gccatcgggc gcgcgatcgg acgctcggca
     1321 aaacgcgcga cgaagtgtac cagcggtttc cgcggctcgc cgagcgtcgc actcagcttg
     1381 cgggcacgct gtcgggcggc gagcgccaga tgctggcgct cggccgcgcg ctgatggccg
     1441 ggccgcgact gctgttgctc gacgagccca gcctcggctt ggcgccgctg atcgtcaagg
     1501 aaatcttcag catcattgcc gcgctaaagg ccagtggcgt gtccattctg ctggtggagc
     1561 agaacgcgcg cgccgcgctg cagattgcca gccacggcta tgttctcgaa acggggacgg
     1621 tcgcgatcgc cgggcccagc gaggcaatat cgcgcgatgc gcgcgtcgcc gcgacgtatc
     1681 tcggacgtac gccggaaagc actagcgcgt cgtaacggcc tcgatggccc ttgtgccggc
     1741 aggcgtgcgc cccgacaatg caggtatccg ccgttttttg agtaaatccc ccgtgaccga
     1801 tctcgtcgtt cctcccgtca agcccgcact gctcgagcag gcgactcgct tattgctcgc
     1861 cccggcagcc cttgagcttc gccagttgtc cgacgtgctt gccggcatcc atggtcatcg
     1921 gatcgatcat gccgacctgt attttcaata ttcacgcgtc gaaagctgga gtctcgagga
     1981 ggggatcgtc aagtcgggct cgttctcgat cgatcgcggc gtgggcgttc gcgcgatctc
     2041 gggagaaaag accgcattcg cttattccga cgatatcagc ctcgatgcat tgacgcaggc
     2101 ggcgacggcg acgcgcgcga tcgggcggca gggtcagtct gccgtcgcgg cgctcgaccg
     2161 gatcggcggg gccagagcgc tctattcttc caacgacccg gtcaccgcgc tgaccgacga
     2221 ggccaaggtg gcactgctcg agcgcctgga acggctggcg cgagcccgcg atccgaggat
     2281 aacgcaggtg atggcatcgc tcgccggtga gtacgaggcg atcctggtcg cgcgcagcga
     2341 cgggtcgatc gccgcggacg tgaggccgct ggtccggatt tcactcaatg tgatcgccga
     2401 g
//
LOCUS       DATANS010000398         6116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_735959, whole genome shotgun sequence.
ACCESSION   DATANS010000398 DATANS010000000
VERSION     DATANS010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735959"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VHT22_05795"
     CDS             complement(<1..266)
                     /locus_tag="VHT22_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX3137101.1"
                     /translation="MDLLITTLLIVALFALLGSGVWIGIALAGVAWIGMELFSSRPAG
                     DAMAVTIWGSASSWTLTALPLFIWMGEILFRTRLSEDMFKGLAP"
     gene            complement(269..781)
                     /locus_tag="VHT22_05800"
     CDS             complement(269..781)
                     /locus_tag="VHT22_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX3137102.1"
                     /translation="MRRSLDALYNAAAFLAALFLVGTLVMVLLGIFGRLLSFQIRGTD
                     AYAGYLMAGAGFLALAHTLMRGEHIRVTLVLEHAGAVGKRWLERWALAVALVLSLAFA
                     WYSVRLAWQSYQFHDISTGNDATPLWIPQLSMAAGNVILAIAFVDLLLAAILGGRTPA
                     VRADEPAHVE"
     gene            complement(841..1812)
                     /locus_tag="VHT22_05805"
     CDS             complement(841..1812)
                     /locus_tag="VHT22_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX3137103.1"
                     /translation="MQRVQILAAAVGLSLALGAAAQTKWDMPTAYPATNFHTENITQF
                     VADVDKATGGRLKITVHANASLFKANEIKRAVQGNQAQAGEILLVNFENEDPMFGIDG
                     VPFLATGYGDAMKLAKASKKALEDRLAKQGIKLLYTVAWPPQGIFTNRTLNSVADLKG
                     LKWRAYSPATSRIAELVGAQPVTVQASELTQALATGVVESYMSSPSTGFDTKTYESIK
                     TFYDTQAWLPKNAVIVNQQAFGALDKATQDAVLKAAADAEVRGWKASEEKNGWYIDQL
                     KQKGMAIVKPSPQLSADLKKVGDTMLQEWLKKSGPDGQAAVDAFRKM"
     gene            2016..5774
                     /locus_tag="VHT22_05810"
     CDS             2016..5774
                     /locus_tag="VHT22_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX3137104.1"
                     /translation="MTDSPQARWQFWIDRGGTFTDIVARRPDGTLATHKLLSENPERY
                     SDAAIAGIRELLGLPPGAPIPVETIEAVKMGTTVATNALLERRGERTVLAITRGFGDA
                     LRIAYQNRPKLFVRRIVLPTMLYERAIEIDERIGAHGDVVRELDLAAAEVALRAAYAD
                     GMRACAIVLMHAYRYPQHEQRLAALARTIGYTQVSSSHEVSPLMKLVSRGDTTVVDAY
                     LSPILRRYVGEVERDLLGPSHSSSAEGPRLQFMQSSGGLTDAHRFQGKDAILSGPAGG
                     IVGAVQVSRQAGFDRIIGFDMGGTSTDVTHYAGEYERAFVTEVAGVRLRAPMMRIHTV
                     AAGGGSICTFDGARFRVGPQSAGANPGPACYRRGGPLTVTDCNLMVGKIDPALFPHVF
                     GPQGDLPLDASVVRERFAALAADVAARSGHRRTPEAIAEGFLQIAVDNMAHAIKHISV
                     ERGYDVTEYTLCCFGGAGGQHACAVADALGMTRVFIHPLAGVLSAYGMGLADVRALRQ
                     RAVEAPLSTAALADAEPVFDELGRVAREEVAAQGIAAGAIAVKRSLHLKYDGTDTTLE
                     IGFGADPSAAPATPGAGHRPDVAALVADFEQRYRTQYGFLMPGKALIIEAAAVEAIGA
                     AAKTDETAPSFPPRAGALRAATINRVYTGGAWHQAPVYARAELRPGDAISGPAVVAEK
                     NATTVIEPGWRATLTAGDHLVLERTTAIARAHAIGTSADPVLLEVFNNLFMAIAEQMG
                     VTLANTAYSVNIKERLDFSCALFDAEGNLIANAPHMPVHLGSMGESVRTVIDRRAGTM
                     QPGDVFVLNAPYNGGTHLPDVTVIAPVFLDEATGSGPDAIRPEFYVASRGHHADIGGT
                     TPGSMPPDSTHVDEEGVLLDNVQLVAQGRFLDDEMRAILGSGRYPSRNIDQNLADLRA
                     QVAACAKGIDELKQMVAHFGLAVVRAYMQHVQDNAEEAVRRVLDVLTDGHFEYEMDSG
                     AKIAVTISIDKAGRKAVIDFIGTSAQQPTNFNAPSAVCKAAVLYVFRTLVDDEIPMNA
                     GCLKPLDIVIPEGSMLAPRYPAAVVAGNVETSQAITDTLYGALGVLAAAQGTMNNFTF
                     GNDTYQYYETISGGSGAGPDFDGESVVQTHMTNSRLTDPEVLEWRFPVRLDEFRIRPG
                     SGGAGAHRGGDGGERRVRFLEPMTAVMLANRRRVPPFGLAGGSAGEPGRNWVERNGGD
                     KEEFGATFQVEMRAGDVFVVRTPGGGGFGAPQDRTDAAGARKPDAGSA"
     gene            5805..>6116
                     /locus_tag="VHT22_05815"
     CDS             5805..>6116
                     /locus_tag="VHT22_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX3137105.1"
                     /translation="MTGVYDFSMTDIHGKPQSLAQYKNKVLLIVNTASQCGFTPQYKG
                     LETLYKKMHARGLEVLGFPCNQFGAQEPGDEKAIAQFCELNYGVTFPLFSKVDVNGED
                     AA"
BASE COUNT         1059 a         2021 c         2025 g         1011 t
ORIGIN      
        1 caaggcgcca ggcctttgaa catgtcctcc gagagccggg tgcggaacag gatctctccc
       61 atccagatga atagcggcag cgcggtcagc gtccacgacg atgccgaacc ccagatcgtc
      121 accgccatcg catcgcctgc cggacgcgac gaaaaaagct ccatcccgat ccatgcgacg
      181 ccggccagcg cgatgcctat ccagacgccg ctgccgagca gcgcgaacag cgcgacgatc
      241 agcaaggtcg tgatcagcag gtccatcgct actcgacgtg cgccggctcg tcggcgcgca
      301 ccgcgggtgt gcggccgccc aaaatcgcgg cgagcagaag atcgacgaag gcgatcgcca
      361 ggatgacgtt gcccgcagcc atcgagagtt gcggaatcca cagcggcgtc gcgtcgttgc
      421 cggtggaaat gtcgtgaaac tgatacgact gccaggcaag ccgcacgctg taccaggcga
      481 acgccagcga cagcacaagc gcgaccgcga gcgcccagcg ctcgagccag cgcttgccga
      541 cggcgccggc gtgctcgagc accagcgtga cccggatatg ctcgccgcgc atcagcgtgt
      601 gcgccagcgc caggaagcct gcgccggcca tcagatagcc ggcgtacgca tcggtgccgc
      661 ggatctggaa actgagcagc cgcccgaaga tgcccagaag caccatcacc agcgtgccga
      721 cgaggaacaa cgccgccaga aaggcggcag cgttgtacag cgcgtcaagc gaacggcgca
      781 tcgttgctgg acgcaggagg atgccggatt actgcgcctg cggggacgga gccggagcgg
      841 tcacatcttc ctgaacgcgt cgacagcggc ctgtccgtct ggtccgcttt ttttgagcca
      901 ttcctggagc atcgtatcgc cgactttctt caggtcggcg ctgagctgcg ggctcggctt
      961 gacgatggcc atgccttttt gcttcagctg atcgatatac cagccgttct tttcctccga
     1021 agctttccag ccacgaactt cggcgtcggc agcggccttg agcacggcgt cctgcgtcgc
     1081 cttgtccagc gcaccgaacg cctgctgatt gacgatcacc gcattcttcg gcagccacgc
     1141 ttgcgtgtcg tagaaggtct tgatgctctc gtaggtcttg gtgtcgaacc cggtcgacgg
     1201 tgacgacatg tacgactcga cgacgccggt ggcgagcgcc tgcgtgagct cggaggcctg
     1261 cacggtcacc ggctgcgcgc cgaccagctc ggcaatgcgc gaggtcgccg gactgtaggc
     1321 gcgccacttc aagcccttca ggtcggcgac gctgttcagc gtccggttgg tgaagatgcc
     1381 ctgcggcggc caggcgaccg tatagagcag cttgatgccc tgcttggcca accggtcttc
     1441 cagcgccttc ttcgacgcct tggcgagctt catcgcatcg ccgtagccgg tggccagaaa
     1501 cgggacgccg tcgatcccga acatcgggtc ctcgttttcg aagttgacca gcaggatctc
     1561 gccggcctgc gcctggttgc cttgcaccgc tcgcttgatc tcgttcgcct tgaacagcga
     1621 cgcgttggcg tgcacggtga tcttgagcct gccgccggtc gccttgtcga cgtcggcgac
     1681 gaactgcgta atgttctccg tatggaagtt ggtcgccggg tacgcggtcg gcatatccca
     1741 tttggtttgc gccgccgcac ccagcgcgag cgacagcccg accgcggcgg cgaggatctg
     1801 aaccctttgc attgaaatct cctgcttgtc caagacgatg gcgagcgaac ttcgcgcgcg
     1861 ggaacgcgcg cccacgtgcg cgggctcaag aacgtgcatt tgtatcatat cggccgatgt
     1921 ggcgaaaccg cttagcgcta gctgaagcgc cgatataatg ggcttcggct tgacgccgtt
     1981 gccaagcgac caaccttgcc ggcaatccct ttccgatgac tgattccccg caggctcgct
     2041 ggcaattctg gatcgaccgc ggcggcacgt tcaccgacat cgtcgcgcgc cggccggacg
     2101 ggacgctcgc gacgcacaag cttttgtcgg aaaaccccga gcggtacagc gacgccgcga
     2161 tcgccggcat ccgcgagctg ctcggcttgc cgcccggtgc gcccattccg gtcgagacga
     2221 tcgaagccgt gaaaatgggc acgacggtcg caacgaatgc actgctcgag cgccggggcg
     2281 agcgcacggt gctggcaatc acccgcggct tcggcgatgc gttgcggatc gcctaccaga
     2341 accggccgaa gctgttcgtg cgccggatcg tcctgccgac gatgctctac gagcgagcga
     2401 tcgagatcga cgagcgcatc ggcgcgcatg gcgacgtcgt ccgcgagctc gatctcgcgg
     2461 cggcggaagt cgccctgcgc gccgcctatg cagacggcat gcgcgcctgc gcgatcgtgc
     2521 ttatgcacgc gtatcgctat ccgcagcacg agcagcggct cgcggcactg gcgcggacga
     2581 taggctacac acaggtctcg tcgagccacg aagtgagccc gctgatgaag ctggtctcgc
     2641 gcggcgacac gacggtggtc gatgcctatc tttcgccgat cctgcgccgc tatgtcggcg
     2701 aggtcgagcg cgatctgctc ggcccgtcgc actcttcgag cgccgaaggg ccgcggctgc
     2761 aattcatgca gtcgagcggc ggcttgaccg atgcgcaccg tttccagggc aaggacgcga
     2821 tcctgtcggg acccgccggt gggatcgtcg gcgccgtgca ggtttcgcgg caggcaggat
     2881 tcgaccggat catcggcttc gacatgggcg gcacgtcgac cgacgtgacg cattacgcgg
     2941 gtgagtacga gcgcgcgttt gtcaccgaag tcgccggcgt acggctgcgc gcgccgatga
     3001 tgcgcattca tacggtggcc gccggcggcg gctcgatctg cacgttcgat ggcgcgcgct
     3061 ttcgcgtagg accgcagtcc gccggcgcca atccgggacc ggcatgctat cgccgcggcg
     3121 ggccgctgac agtcaccgac tgcaatctga tggtcggcaa gatcgacccg gcattgttcc
     3181 cgcacgtgtt cggcccgcaa ggcgatctgc cgctcgatgc atccgtcgtg cgcgagcgtt
     3241 tcgccgcgct ggcggccgac gtcgccgccc ggtccggaca ccggcgaact ccggaagcga
     3301 tcgccgaagg cttcctgcag atcgcggtcg acaacatggc gcacgcgatc aagcacattt
     3361 cggtcgagcg cggttacgac gtcaccgagt acacgctgtg ttgcttcggc ggcgccggcg
     3421 gccagcatgc ctgtgccgtc gccgacgcgc tgggcatgac gcgggtattc atccatccgc
     3481 tggccggcgt gctgtccgcc tacgggatgg gtctcgccga cgtgcgggcg ctgcgccagc
     3541 gcgcggtcga ggcgccatta tcgacggcag cgctcgccga cgccgagccg gtgttcgatg
     3601 agctcgggcg ggtggctcgc gaagaggtcg ccgcacaagg catcgcagcc ggtgcaatcg
     3661 ccgtcaagcg ctcgctgcat ctcaaatatg acggcaccga tacgacgctg gaaatcggct
     3721 tcggcgccga tccctcggcc gccccggcga cgccaggtgc ggggcaccgc cccgacgttg
     3781 cagcgctggt cgccgacttc gagcagcgct atcggacgca atacggcttc ctgatgccag
     3841 gcaaggcgtt gatcatcgaa gccgccgcag tcgaagcgat cggcgccgcg gcgaagaccg
     3901 acgagaccgc gccatcgttc ccgccgcgcg ccggcgcatt gcgagccgcg acgatcaacc
     3961 gcgtatacac cggcggcgca tggcaccagg cgccggtata tgcccgcgcc gagctgcggc
     4021 ccggagacgc gatctccggt cccgcggtcg tcgccgaaaa gaacgcgacg acggtgatcg
     4081 agccggggtg gcgggcgacg ctgaccgccg gcgatcactt ggtgctcgag cggacgacgg
     4141 cgatcgcgcg cgcgcatgcg atcgggacca gcgccgaccc ggtgctgctc gaggtgttca
     4201 acaacctttt catggcgatt gccgagcaga tgggcgtgac gctcgccaat accgcgtact
     4261 cggtcaacat caaggagcga ctcgattttt cgtgcgcgct gttcgacgcc gaaggcaatc
     4321 tgatcgccaa cgccccgcac atgccggtgc acctgggctc gatgggagag agcgtcagga
     4381 cggtcatcga tcgccgcgcc ggcacgatgc agccgggcga cgtgttcgtg ctcaacgcgc
     4441 cgtacaacgg cggcacgcat ctgcccgacg tcacggtgat tgcgccggtt tttctcgacg
     4501 aggcgactgg gagcggacca gacgccattc gcccggagtt ctacgtcgcc tcgcgcggcc
     4561 atcacgccga catcggggga acgacgccgg gatcgatgcc gccggattcc acccacgtcg
     4621 acgaggaggg cgtgctgctc gacaacgttc agctggtcgc gcagggacgc tttctcgacg
     4681 acgaaatgcg cgcgatcctg ggctcggggc gctatccatc gcgcaacatc gaccagaacc
     4741 tcgccgacct gcgggcgcag gttgcggcgt gcgccaaggg catcgacgag ctcaagcaga
     4801 tggtcgcgca cttcggcctc gcggtcgtgc gcgcctacat gcagcatgtg caggacaacg
     4861 ccgaggaggc ggtgcgccgc gtgctcgacg tattgaccga cggccatttc gaatacgaaa
     4921 tggatagcgg cgcgaagatc gcggtcacga tctcgatcga caaggcagga cgcaaggcgg
     4981 tgatcgattt catcgggacc agcgcccagc agccgaccaa tttcaacgcg ccgtcggcgg
     5041 tgtgcaaggc ggcggtgctg tacgtctttc gcacgctggt cgacgacgag attccgatga
     5101 acgcaggttg cctgaagcca ctcgacatcg tcattccgga aggatcgatg ctcgcgccgc
     5161 ggtatccggc cgcggtcgtc gccggcaacg tcgagacgtc gcaggcgatc accgacacgc
     5221 tctacggcgc gcttggcgtc ctcgccgcgg cgcagggcac gatgaacaat ttcacgttcg
     5281 gcaacgacac ctaccagtat tacgagacga tctccggcgg ttcgggcgcc gggccggact
     5341 tcgacggcga gagcgtcgtg cagacgcaca tgaccaactc ccgtctgacc gatccggagg
     5401 tgctcgaatg gcgctttccg gtgcggctcg acgagtttcg cattcgtccc ggcagcggcg
     5461 gcgccggcgc ccaccgcggc ggcgacggcg gtgagcgccg cgtccgcttc ctcgaaccga
     5521 tgaccgcggt catgctcgcc aaccgcaggc gggtgccgcc gttcggcctg gccggcggca
     5581 gcgccggcga gccggggcgg aactgggtcg agcgcaacgg cggcgacaaa gaggagttcg
     5641 gcgcaacctt tcaggtcgaa atgcgcgccg gcgacgtctt tgtggtccgg acgccaggtg
     5701 gcggcggctt cggcgcgccg caggatcgga ccgacgcggc gggcgctcgc aagccggatg
     5761 ccggcagcgc ataattctcc catccatttt ttcgagagat tccgatgaca ggcgtctacg
     5821 atttttcgat gaccgatatc cacggcaagc cgcagtcgct tgcccaatac aagaacaagg
     5881 tgttgctgat cgtcaacacc gcgagccaat gcggattcac gccgcagtac aagggcctcg
     5941 agacgctgta caagaaaatg cacgcgcgcg ggctggaagt gctgggcttt ccctgcaacc
     6001 agttcggcgc gcaggagccc ggcgatgaaa aagcgatcgc gcagttttgc gagctcaact
     6061 atggcgtgac gtttccgctc ttctccaagg tcgatgtcaa cggcgaagac gcggcg
//
LOCUS       DATANS010000399         2438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_147149, whole genome shotgun sequence.
ACCESSION   DATANS010000399 DATANS010000000
VERSION     DATANS010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147149"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VHT22_05820"
     CDS             <1..761
                     /locus_tag="VHT22_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional glutamine synthetase
                     adenylyltransferase/deadenyltransferase"
                     /protein_id="HEX3137106.1"
                     /translation="ELGYASDLDLVFLYDDPAEAAPQAYARLAHRLTTWLTSTTAAGR
                     LYDTDLRLRPDGASGLPVSSLTAFRNYQRNTAWTWEHQALTRARYVAGDARIGAAFEA
                     ERDAVLRLPRDPVKLAADIVAMRNRMQAAHPNRSGLFDLKHDPGGMVDVEFAVQFLVL
                     AHSHAHPEMTRNAGNIALLALAASLALLPSDLASAVADAYREYRRLQHQVRLQGAPEA
                     RVEALPQSARRETVAILWKQIFGAPRSENAHEIS"
     gene            794..1720
                     /locus_tag="VHT22_05825"
     CDS             794..1720
                     /locus_tag="VHT22_05825"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX3137107.1"
                     /translation="MSMSDRDGFIWYDGKLVPWRDANTHVLTHSLHYGLAVFEGVRAY
                     KTVDGPAIFRLAEHTERLFNSARIYLMQLPYGKDVLMAAQREVVRANKLESCYLRPIA
                     FYGSEKMGVSPKGAKVHVAIAGWPWGAYLGPEALDKGIRVKTSSFARHHINVTMARAK
                     FAGTYANSILANQEATMDGYDEALLLDVDGFVAEGSGENLFIVKNGRLYEPELTSALV
                     GITRDSVITLARDIGLEVSAKRLTRDDVYIADEAFFTGTAAEVTPIRELDNRTIGSGE
                     CGPITRKLQSLFFDAVHGKNAKYRSWLTAVQS"
     gene            1720..1917
                     /locus_tag="VHT22_05830"
     CDS             1720..1917
                     /locus_tag="VHT22_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEX3137108.1"
                     /translation="MSVISPPQSAVEVTAADLPLYCPNPAMPLWSSHPRVFLDVAATG
                     EAMCPYCGTRYHMKGPPPRGH"
     gene            1978..>2438
                     /gene="waaF"
                     /locus_tag="VHT22_05835"
     CDS             1978..>2438
                     /gene="waaF"
                     /locus_tag="VHT22_05835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEX3137109.1"
                     /translation="MAEPILLVAPSWIGDAILSEPLLAILREPLQEPTVDVLAPPWCA
                     PVYARMRGVRNIIENPIGHRELRLGERRALARTLRAAGYTRALVLPNSFKSALIPWMA
                     RIPRRIGYAREARRILLTDARRLDSKALPRLVDRFVALAAQPGELVPTSAAP"
BASE COUNT          424 a          801 c          786 g          427 t
ORIGIN      
        1 aggagctcgg ctacgcttcg gacctcgacc tcgtgttcct gtacgacgat cccgccgagg
       61 ccgcaccgca ggcctacgcg cgtcttgcgc atcggctcac gacctggctg accagcacga
      121 ccgcggcagg gagactgtac gacaccgatc tgcggcttcg tcccgacggc gcgagcggcc
      181 tgccggtgtc gagtctgacc gcgtttcgca actatcagcg caatacagcg tggacctggg
      241 agcaccaggc gctgacccgc gcccgctacg tcgccggtga cgcgcgcatc ggcgcggcat
      301 tcgaggccga acgcgacgcg gtccttcgtc tgccgcgcga cccggtcaag ctcgccgccg
      361 acatcgtcgc gatgcgcaac agaatgcagg cagcgcatcc gaatcgaagc gggctcttcg
      421 acctcaagca cgatccgggc ggcatggtcg acgtggaatt tgccgtgcag ttcctggtcc
      481 tggcgcacag ccacgcgcat ccggaaatga cgcgcaacgc cggcaacatc gcgctgctcg
      541 cgctggccgc cagtctcgcg ctgctgcctt cggacctcgc ctcggcagtc gccgacgcgt
      601 accgcgaata tcggcggcta cagcaccagg tccgccttca gggcgccccc gaagcgcgcg
      661 tcgaggcgct tccgcagtca gcccgccgcg aaactgtcgc gatactttgg aaacaaatct
      721 tcggtgcgcc tcggtcggaa aacgcccacg aaatcagcta agatgttggt tttgccaagc
      781 gcggagctgc aggatgtcga tgtcggatcg tgacgggttc atctggtacg acggaaagct
      841 ggtgccgtgg cgcgacgcca acacgcacgt gctcacgcat tcgctgcact acggactcgc
      901 ggtgttcgag ggcgtgcgcg cgtacaagac ggtcgacggg ccggccatct tcaggctggc
      961 cgagcacacg gagcgtctgt tcaattcggc ccgcatttat ctgatgcagc ttccgtatgg
     1021 caaagacgtg cttatggcgg cgcagcgcga agtcgtgcgc gccaacaagc tcgagtcctg
     1081 ctatctgagg ccgatcgcgt tctacggctc ggaaaagatg ggcgtgtcgc cgaagggagc
     1141 caaggtccat gtcgccattg ccgggtggcc atggggcgcc tacctcggcc ccgaagcgct
     1201 ggacaagggg atacgcgtaa agacgtcgtc gttcgcgcgg catcacatca acgtgacgat
     1261 ggcgcgggcc aagttcgccg gcacctacgc caattcgatt ctggccaacc aggaagcgac
     1321 gatggatggc tacgacgagg ccttgctgct cgatgtcgac ggtttcgtcg ccgaaggctc
     1381 gggtgagaac ctgttcattg tcaagaacgg acgactctac gagcctgagc tgacctcggc
     1441 gttggtcggc atcacccgcg attccgtgat cacgctggcg cgcgacatcg ggctcgaggt
     1501 gagcgccaag cgcctgacgc gcgatgacgt atacatcgcc gatgaggcat ttttcaccgg
     1561 caccgccgcc gaggtgacgc cgatccgcga gctcgacaat cgcacgatcg gatccggcga
     1621 gtgtggaccg atcaccagga agttgcagag tctgttcttc gatgccgtgc acggcaagaa
     1681 cgccaagtat cgatcctggc tcaccgccgt ccagagctga tgagcgtgat aagcccgccg
     1741 caaagtgctg tcgaagtcac ggcagcagac ctgccgctgt attgtccgaa ccctgcgatg
     1801 ccgctgtggt cgagccatcc ccgtgtcttt ctcgatgtgg ccgctaccgg cgaggcgatg
     1861 tgtccgtatt gcggcacgcg gtaccacatg aagggcccgc cgccacgcgg acattgagag
     1921 agttcatcgt agcgatcgtg tgccgggggc gcgaacgatc gtgccgggac cgacaccatg
     1981 gctgaaccca tcctgctcgt cgcaccctcg tggatcggcg atgccatctt gtccgagcct
     2041 ctgctcgcta tcctgcgcga gcctctgcag gagccgaccg tagacgttct cgcgccgccg
     2101 tggtgcgcgc cggtctatgc gcgcatgcgc ggcgtgcgca atatcattga aaatccgatc
     2161 ggccaccgcg aattgcggct gggcgagcgt cgtgcgctgg cacgcacgct tcgcgctgcc
     2221 ggttacacgc gggcgttggt gttgccgaat tcgttcaagt cggcgctgat cccgtggatg
     2281 gcgcgcattc cgcgccgcat cggttacgcc agggaagcgc gccggatcct gctcacggat
     2341 gcccgacggc tcgacagcaa agccctgccg cgcctggtcg accggttcgt ggcactcgcg
     2401 gcgcaaccgg gcgagctggt gccgacctcg gcagctcc
//
LOCUS       DATANS010000400         1633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_356887, whole genome shotgun sequence.
ACCESSION   DATANS010000400 DATANS010000000
VERSION     DATANS010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356887"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1586
                     /gene="glgA"
                     /locus_tag="VHT22_05840"
     CDS             123..1586
                     /gene="glgA"
                     /locus_tag="VHT22_05840"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEX3137110.1"
                     /translation="MPLAPLRILYATPECAPLVKTGGLGDVAGALPIALRRIGLDARV
                     LLPGYRPVLAALPGARDAGRIESFARLPSARLLEAELPQGVPAWIVDCPALYDRDGGP
                     YQDTSGVDWEDNPLRFGLLSHVAARLASAQSPVAWRAQLVHANDWQTGLAPAYAALAL
                     GGATPSIITIHNLAFQGNFAPHWVADLGLPATSFAVDGVEYYGHLSFLKAGIFFANAI
                     TTVSPTYAQEIQTEAMGMGLHGLLTTRRDSLTGILNGIDTTVWDPESDASIPERYDAT
                     TLQRKAANKRALQIRFGLDPLGDVPLLGAVTRLTPQKGIDLLLDIVDAVLALPVQIVI
                     VATGDKGLEQQLRALTDARPGHLASFIGFDETLAHLVEAGADAFVMPSRFEPCGLNQM
                     YSQRYGTPPIVHATGGLVDSVVDCTPHSLAAGTATGFKFFAPNPEALKSAIARCVAAY
                     RDPAVWRALQRNGMARDFGWNTAAREYAALYERTVSR"
BASE COUNT          262 a          544 c          525 g          302 t
ORIGIN      
        1 agcattcagg tcgcccgaag accgattgcc gatctgttat attcgaacgg gttcggcacc
       61 gactcgaggt cgagcgggcg ttttcccgtg aggacgcgcc cagcgatcga acaagctcgc
      121 cgttgccatt ggctccgctt cgcatactct acgcaacgcc ggaatgcgcg ccgctggtca
      181 agaccggcgg tctcggtgac gtcgcaggcg cgcttccgat cgcgctgcgt cggatcgggc
      241 tggacgcccg cgtcctgttg cccggatacc ggcccgtgct cgctgcgctg cccggcgcgc
      301 gtgacgcggg acgcatcgaa tcgttcgcgc ggcttccgtc cgcgcgcctt ctcgaggccg
      361 agttgcccca gggcgtcccg gcgtggatcg tcgattgtcc ggcgctctac gatcgcgacg
      421 gcggcccgta ccaggacacg agcggtgtcg actgggaaga caatccgctg cgcttcggct
      481 tgctgtcgca cgtcgcggct cggctggctt cggcgcagag tcccgtcgcg tggcgcgcgc
      541 agctcgttca cgcgaacgac tggcagaccg gacttgcgcc ggcgtacgcc gcgcttgccc
      601 tcggcggcgc aacaccaagc atcatcacga tccacaatct tgcgttccag ggaaatttcg
      661 cgccgcattg ggtcgccgat ctcggattgc ccgcgactag cttcgctgtc gacggcgtcg
      721 aatactacgg ccacctttcc tttctcaagg ccggcatctt tttcgccaac gccatcacga
      781 cagtcagtcc gacttacgcc caggagatcc agacggaggc gatgggcatg ggcttgcacg
      841 gtctgcttac gacgcgtcgc gacagtctca ccgggattct gaacggtatc gatacgactg
      901 tctgggatcc ggagagcgat gcgtcgatcc ccgagcgata cgatgcgacg accttgcaac
      961 gcaaggccgc caacaagaga gcgttgcaga ttcgcttcgg cctcgacccg cttggcgacg
     1021 ttccactact gggcgcggtg acgcggctga ccccgcagaa gggaatcgat cttctgctcg
     1081 atatcgtgga cgccgttttg gcgctgccgg tccagatcgt catcgtcgcg accggggaca
     1141 agggcctgga acagcagctg cgcgcgctta cggacgctcg tccggggcac ctggcgagct
     1201 tcatcggctt cgacgaaacg ctcgcacacc tcgtcgaagc gggcgccgat gcgttcgtca
     1261 tgccgtcgcg cttcgagccc tgcggattga accagatgta cagccaacgc tacggcacac
     1321 cgccgatcgt ccacgcgact ggcgggctgg tcgattcggt cgtcgattgc acgccgcatt
     1381 cactcgccgc cggcaccgcg acgggattca agttttttgc accgaatccc gaggctctca
     1441 agagcgcgat cgcgcgctgt gttgccgcct atcgcgatcc cgcggtgtgg cgcgcgctgc
     1501 agcgaaacgg gatggcgcgc gacttcgggt ggaacaccgc cgcgcgggaa tacgccgcgc
     1561 tctatgaacg gaccgtgtcg cgctagcctg ttcgctcgac tgggcgagga aacgcttcgg
     1621 cgttagctag ggg
//
LOCUS       DATANS010000401         2060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_147171, whole genome shotgun sequence.
ACCESSION   DATANS010000401 DATANS010000000
VERSION     DATANS010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147171"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VHT22_05845"
     CDS             complement(<1..470)
                     /locus_tag="VHT22_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014724979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3137111.1"
                     /translation="MNKPASKLVVEDLHLQYGDNKILQGVSMTLNPGEVVALLGASGS
                     GKTTLLRSVAGLEQPSRGRISIGDTVLYDGANGVEMPVEKRNLGLVFQSYALWPHRSV
                     FDNIAYGLRLRGVPASEIDSRVNLALTNLGLGALATRLPHQLSGGQQQRVAIARA"
     gene            complement(463..>2060)
                     /locus_tag="VHT22_05850"
     CDS             complement(463..>2060)
                     /locus_tag="VHT22_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX3137112.1"
                     /translation="FWQSFLNAPFFNPQRHIGFDAYEFIFADADFWTALFNSVVIAAG
                     MVLIALPMGAALAFLLTRTDLPAQRVIEVLVLIPVFVSPMVLAFGYVVSMGPVGFYSL
                     WAKQLVGDVPWNVYSLTSIAVIAGLTHVPHVYLYSSAALKSLGSDVEEAARVVGANPL
                     KVALNVSLPMTMPALLYAGVLVFFLGFELFGLPLVLGDPEGHLVLSTYLYKLTNKLGT
                     PSYHLMAAVAVCIVAITFPLVLLQRWLLKTAGKYVSVKGKATRQKLLPLGAWKWLALA
                     IVALWLVFTIVVPISGIALRAFVEQWGEGINLGDVLTLANFRQVFDQSDQVRAIVNTF
                     LIGTIGGGLAVACYTAIGLAGHRQQGGVARVLDYLVLIPRAVPGLLAGLAFLWIFLFV
                     PGLKELRNTVFSVWLAYTVVWLAYGLRLISSALLQIGPELEEAASTAGASRAQVMREV
                     TLPLIRFGLLASWLLVFMIFEREYSTGVYLLGQGTEVIGSMLVSLWGAGAIDQVAALS
                     LINIALVAVGLAAALRFGVRIHE"
BASE COUNT          427 a          700 c          644 g          289 t
ORIGIN      
        1 gcgcgcgcga tcgcgacgcg ctgctgctgg ccgcccgaaa gctgatgcgg cagccgcgtg
       61 gcaagcgcgc cgaggccaag attggtcagc gccagattga cgcggctgtc gatttcactg
      121 gccggtacgc cgcgcaggcg caacccgtag gcgatgttat cgaacacgct ccgatgcggc
      181 cacagtgcgt acgattggaa gaccaggccc aggttgcgct tttccacggg catctcgaca
      241 ccgttggcgc cgtcgtagag cactgtgtcg ccgatcgaga tccgaccccg gctcggctgc
      301 tcgagaccgg caaccgagcg cagcagcgtc gtcttgccgc tgccggaggc gcccagcagc
      361 gcgaccacct cgcccgggtt cagggtcatc gaaacgccct ggagaatctt gttgtcgccg
      421 tattgcagat gcaaatcctc gacgacgagc ttgctggcgg gcttattcat ggatacgcac
      481 tccgaaacgc agggccgccg cgagaccgac ggcgaccagc gcgatattga tcagcgacag
      541 cgcggcgacc tggtcgatcg cgccggcacc ccacagcgaa acgagcatcg agccgatgac
      601 ttcggtgccc tgccccagca ggtacacacc ggtcgagtat tcgcgctcga agatcatgaa
      661 caccagcagc cagctcgcca gcaatccgaa ccggatcagc ggcaaggtca cctcgcgcat
      721 cacctgcgcg cgcgatgcgc ctgcagtgct cgccgcctcc tcgagctccg gcccgatctg
      781 cagcagcgcg ctggagatca gtcgcaatcc gtacgccagc caaacgaccg tgtaggcaag
      841 ccacaccgag aacaccgtgt tgcgcaactc cttcaggcct ggcacgaaca gaaagatcca
      901 gaggaaggcc agccccgcca gcaagcccgg caccgcgcgc gggatcagca ccagatagtc
      961 gagcacccgg gcgacgccgc cctgctggcg gtggccggcc agtccgatcg ccgtatagca
     1021 ggcgaccgcg agtccgccgc caatcgtgcc gatcaggaag gtattgacga tagcgcgcac
     1081 ctgatcgctc tggtcgaaca cctgccggaa gttggcgagc gtcagcacgt cgccgagatt
     1141 gatgccttcg ccccattgct cgacgaatgc gcgcaacgcg atgcccgaga tcggcacgac
     1201 gatggtgaac accagccaca gcgcgacgat cgccagcgcc agccacttcc acgcgccgag
     1261 cggcaagagc ttctgccgcg tcgccttccc cttgaccgag acgtacttgc cggcggtctt
     1321 gagcagccag cgctgcagca ggaccagcgg aaaggtgatc gcgacgatgc acaccgccac
     1381 cgcggccatc aggtgatacg agggcgtccc gagcttgttc gtcagcttgt acaggtaggt
     1441 cgacagcacc agatggcctt ccgggtcgcc cagcaccagc ggcaggccga acagctcgaa
     1501 gccgaggaag aagaccagca cgccggcgta gagcagcgcc ggcatcgtca tcggcaggct
     1561 gacgttgagc gccaccttca gcggattggc gccgacgacc cgcgccgcct cctcgacgtc
     1621 cgagcccagg cttttgagcg cggcggaaga atacaggtaa acgtgcggca cgtgcgtcag
     1681 cccggcaatg accgcgatgc tggtcagcga gtacacgttc cacggcacgt cgccgacgag
     1741 ttgcttggcc cacagcgaat agaacccgac cggacccatc gagacgacgt agccgaacgc
     1801 cagcaccatc ggcgaaacga acaccggtat cagcaccagc acttcgatga cccgctgcgc
     1861 gggaagatcg gtgcgcgtca gcaggaacgc cagtgcggcg cccataggca gcgcgatcag
     1921 caccatcccc gcggcgatca ccaccgaatt gaaaagcgcc gtccagaaat cggcgtcggc
     1981 gaatatgaac tcgtaggcgt cgaatccgat gtggcgctgc ggattgaaga acggcgcgtt
     2041 gaggaagctc tgccagaaga
//
LOCUS       DATANS010000402         2862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_104568, whole genome shotgun sequence.
ACCESSION   DATANS010000402 DATANS010000000
VERSION     DATANS010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104568"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="VHT22_05855"
     CDS             complement(<1..1153)
                     /locus_tag="VHT22_05855"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3137113.1"
                     /translation="MNIDHASWQRLSPLLDEALDLPAEQRTQWLVELRGRDPELAEEV
                     EALLDEARAIGDERFLEHTPEGPERATLAGQTIGAYTLEAPLGHGGMGSVWLARRSDG
                     RFEGKVAIKFLNAALVGRTGEERFRREGSILARLTHPNIARLIDAGVSATGQPYLVLE
                     LVDGKPIDQYCDDQRFDVAARLKLFLDVLAAVAHAHAHLVVHRDLKPSNVLVTNDGTV
                     KLLDFGIAKLLQDELHPSEATELTREAGRAMTPEYAAPEQLLGLPVTTATDVYALGVL
                     LYVLLGGQHPAGGSTRSSADLVKAIVETSPTRLSEAVTSAKTLDGQALLDNAAKRAAT
                     PERLAGVLRGDVDNIVGKALKKNPIERYASVTAFADDIRRYLDHEPVSAR"
     gene            complement(1226..1789)
                     /locus_tag="VHT22_05860"
     CDS             complement(1226..1789)
                     /locus_tag="VHT22_05860"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX3137114.1"
                     /translation="MELDLTALFAAAEKGDAAAREQLFQRLYAELHRLARTELWRSGA
                     GVSMGATTLLHEAFINMSERDATAFPDRARFMAYAARAMRGLIIDHARSRHAQKRGGQ
                     FDITALDTNICNETADQMDLPQVGDALDGLAKLEPDLAQVVDLKFFCGFSFAEIAAMR
                     GVSERTVQRDWEKARIYLRRSLREGSM"
     gene            complement(1900..2724)
                     /locus_tag="VHT22_05865"
     CDS             complement(1900..2724)
                     /locus_tag="VHT22_05865"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEX3137115.1"
                     /translation="MQVAGASPERDSPRAAFALLSWRVNLTVVVALFALSAFAWRSTI
                     QDAVSMRGMVMGFGQIGWLAQGDMSAGVFLAMWVTMMVAMMLPAVAPMVLAHLGVTRR
                     RGRGVVPTLVFIAGYLLVWSAIGAVPLLVYKAFAPLSDDPALLRWLPALAGTILIVAG
                     AYQFSAWKRICLDHCQTPFAFLASHDFAGGSLSALRAGLVHGTLCLGCCWAIMTVLLL
                     VGLMNLLWMAAIFALIVVEKNWKYGLVAAKVAGVALMVLGAAVIARPGLLAAISLP"
BASE COUNT          507 a          984 c          935 g          436 t
ORIGIN      
        1 cgcgcgcgct gaccggttcg tgatcgagat agcgccggat gtcgtcggcg aacgcggtca
       61 ccgacgcata gcgctcgatc ggattcttct tcagcgcctt gccgacgatg ttgtcgacat
      121 cgccgcgcag gacgccggcg aggcgctccg gcgtggccgc gcgttttgcc gcgttgtcga
      181 gcagcgcctg accgtcgagc gtcttcgccg acgtgacggc ttccgagagc cgggtcggcg
      241 aagtctcgac gatcgccttg acgaggtccg ccgaggaacg cgtgctgccg ccggctggat
      301 gctggccccc gagcagcacg tagagcaaca cgccgagggc gtagacgtcg gtcgcggtcg
      361 tgaccggcaa gcctaagagc tgctcgggcg cggcgtattc gggtgtcatc gcgcgccccg
      421 cttcgcgcgt gagttcggtg gcctcgctgg ggtgcaactc gtcctggagc agcttggcga
      481 tgccgaaatc gagcagcttc accgtgccgt cgttggtcac cagcacattc gacggcttca
      541 ggtcgcgatg cacgacgaga tgcgcgtggg catgcgcgac cgccgccaga acgtcgagaa
      601 agagcttcag ccgcgcagcg acgtcgaagc gctggtcgtc gcaatactgg tcgatgggct
      661 tgccatcgac gagctcgagc accagatacg gctggcccgt ggcggagacg ccggcgtcga
      721 tcaatcgcgc gatgttggga tgggtcagcc gcgcgagaat gctgccttcg cggcggaagc
      781 gctcctcgcc ggtgcggccc accagcgcgg cgttgaggaa cttgattgca accttgccct
      841 cgaagcggcc atcactgcgg cgggcgagcc agacgctgcc catcccgccg tgtcccagcg
      901 gcgcttcgag cgtgtacgcg ccgatcgtct ggccggcaag cgtcgcccgc tccgggcctt
      961 cgggcgtgtg ctcgagaaag cgctcgtcac cgattgcgcg cgcttcgtcg agcagcgcct
     1021 cgacctcttc ggcaagctcc gggtcgcgcc ctcgcagctc cacgagccac tgcgtccgct
     1081 gttccgccgg caaatccaga gcctcgtcga gcaatgggct cagccgctgc cagctcgcgt
     1141 gatcgatatt cactccggca tcctcggctt caaatatctg tatatcggaa ttcgaccgcg
     1201 aaaaccgaca aggccccgat catggctaca tcgaaccctc gcgcaggctc cgacgcagat
     1261 agattcgcgc cttctcccag tcgcgctgca cggttcgctc ggaaaccccg cgcatcgccg
     1321 cgatctccgc gaaggaaaag ccgcagaaga acttaaggtc aaccacctgc gccagatcgg
     1381 gctcgagctt ggctaggccg tcgagcgcgt cgccgacctg cggaagatcc atctggtccg
     1441 ccgtctcgtt gcagatgttg gtgtcgagcg cggtgatgtc gaactggccg ccgcgcttct
     1501 gcgcatggcg gctgcgcgca tggtcgatga tcaggccgcg catcgcccgc gcggcgtagg
     1561 ccatgaagcg ggcacggtcg ggaaaggcgg tcgcgtcgcg ctctgacatg ttgatgaacg
     1621 cctcgtgcag caaggtcgtc gcgcccatcg aaaccccggc gccgctgcgc caaagctcgg
     1681 ttcgggctag ccgatgcagc tcggcataga gccgctggaa gagctgctcc cgcgcggccg
     1741 cgtcgccttt ttccgcggcg gcgaagagcg cggtcaaatc gagttccacg tcgtgcgccc
     1801 ccggatgggc agtacgcccc gggaaccaaa aagtatatgc cctgcggcgc gagtcagctg
     1861 tcaggaaacc cagccaacgg gtggaatgta cggcgagtgc tacggcagcg aaatcgccgc
     1921 aagcaacccc ggacgggcga tcaccgccgc acccagcacc atcagtgcaa cgccggccac
     1981 cttcgccgcg accaggccgt acttccagtt cttctcgaca acgatcagcg cgaagatagc
     2041 ggccatccac aacaagttca tcagcccgac cagcagcagc acggtcatga tcgcccagca
     2101 gcagccgagg cacagcgtgc cgtggaccag tccggcgcgc agcgcgctga gcgagccgcc
     2161 ggcgaaatcg tgcgacgcaa gaaaggcgaa gggcgtctgg caatggtcga gacagatacg
     2221 cttccacgcg ctgaattgat acgcgccggc gacgatcaga atcgtgccgg ctaacgctgg
     2281 cagccaccgc agcagcgccg gatcgtcgct caacggcgcg aaggccttgt agaccaggag
     2341 cggcaccgcg ccgatcgccg accacaccag caggtagccg gcgatgaaca ccagcgtggg
     2401 aacgacgccg cggccgcgcc ggcgcgtgac gccgagatgc gcgagtacca tcggcgcgac
     2461 cgccggcagc atcatcgcga ccatcatcgt cacccacatc gcgagaaaga cgccggcgct
     2521 catgtcgccc tgggccagcc agccaatctg cccgaatccc atcaccatgc cgcgcatcga
     2581 gaccgcgtcc tggatcgtgc ttcgccacgc gaacgccgac agcgcgaaca gggcgacgac
     2641 gaccgtcagg ttgacccgcc acgacaacag cgcgaacgcc gcgcgtggcg agtctcgttc
     2701 gggcgatgcg ccggcaactt gcatcggtca atccgggccg cgccagctga acgcgaggtg
     2761 cttgctcgat ttgccgctgc ggctccactg gaagccgaac atgtcgaccc ggtccgcggt
     2821 ggcggtgccc caggtcgcca cggtgccgct ggggccgacc tc
//
LOCUS       DATANS010000403         1618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_229904, whole genome shotgun sequence.
ACCESSION   DATANS010000403 DATANS010000000
VERSION     DATANS010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229904"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VHT22_05870"
     CDS             <1..211
                     /locus_tag="VHT22_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137116.1"
                     /translation="GAITLYTVKDGALVPLAMNVGGKTEKLAAAPAAAAPAPDAMKSA
                     APKADAMKSEAPKADAMKSEAPAKK"
     gene            378..1373
                     /locus_tag="VHT22_05875"
     CDS             378..1373
                     /locus_tag="VHT22_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX3137117.1"
                     /translation="METFLQQIINGLTLGSVYALVALGYTMVYGIIGLINFAHGEVVM
                     IGAMVALSVITALVGSHAGLPAVVIILAGLLVAILICVAIAYSMEKIAYRPLRRAPRL
                     APLITAIGMSIVLQNVALMVWGRNYRTFPTLIQPTTHNILGAAMTDLQMLIVVLAALL
                     MGGLVLIVQRTRLGMAMRATAQNPQVAGLMGIDINVIISAAFVIGAALAAVAGLMVMA
                     YYGIAHYYMGFLLGLKAFTAAVLGGIGNLAGAMLGGILLGIIEALGAGYIGDVSNVCG
                     LSSLSDTLQQKCAASDGQVSLFGSNYQDVFAFFVLILVLIFRPSGLLGERVADRA"
     gene            1370..>1618
                     /locus_tag="VHT22_05880"
     CDS             1370..>1618
                     /locus_tag="VHT22_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3137118.1"
                     /translation="MNNAGRTGIPLQVWAGVALVAIALGVLPFVAALAGQAWVRILNF
                     AMVYTMLALGLNIVVGFAGLLDLGYIAFYAVGAYTYALL"
BASE COUNT          249 a          521 c          543 g          305 t
ORIGIN      
        1 cggcgcgatc acgctgtaca cggtcaagga cggcgcgctg gtgccgttgg cgatgaatgt
       61 cggtggcaag accgagaagc tcgcggcggc gccggcggcg gcagcaccgg ccccggacgc
      121 gatgaagtcc gcggcgccca aggccgatgc aatgaagtcc gaggcaccca aggcggatgc
      181 gatgaagtcc gaagcgccgg caaagaaata gtcgtcgcgg ctcgtccgct gtcgcaacac
      241 cgaaacggcg tccctcgggg cgccgtttcc atttcaaccg tccggcgtcg gcgcccgggg
      301 gattgccgcg gcgttggcca cagcggagaa tcggccacta gccgggggcc gccgcggcgc
      361 gacgggagat tcgcttcgtg gaaacctttc tgcagcagat catcaacggt ctcacgctcg
      421 gcagcgtcta tgcgctggtt gcgctcggct acacgatggt ctacggcatc attggcctga
      481 tcaatttcgc gcatggcgaa gtggtaatga tcggagcgat ggtcgcgctg tcggtcatca
      541 ccgcactggt tggctcgcac gccgggcttc ccgcagtcgt gatcatcctc gccggcctcc
      601 tggtcgcgat cctcatctgc gtggcgatcg cgtacagcat ggaaaagatc gcctaccggc
      661 cgttgcggcg cgccccgcgc ctcgcgccgc tgatcacggc catcggcatg tcgatcgtgc
      721 tgcagaacgt cgcgctgatg gtctggggcc gcaactaccg caccttcccg acgctgatcc
      781 agccgacgac gcataacatc ctcggtgcgg cgatgaccga tctgcagatg ctgatcgtcg
      841 tcctggcagc gctgctgatg ggcgggctcg tgcttatcgt gcaacgtact cgcctcggga
      901 tggcgatgcg ggccaccgcg cagaacccgc aggttgcggg cctgatgggc atcgacatca
      961 acgtgatcat ctcggcggcg ttcgtcatcg gggccgcgct ggctgccgtg gccggcctga
     1021 tggtgatggc ctactacggc atcgcgcact actacatggg cttcctgcta ggcctgaagg
     1081 cattcaccgc tgcggtgctg ggcggcatcg gcaacctcgc cggggcgatg ctgggaggca
     1141 tactgctcgg catcatcgag gcgctgggcg ccggttacat aggcgacgta tccaacgtct
     1201 gcggcctgtc gtcgttgagc gatacgctgc agcagaagtg cgcggccagc gatgggcagg
     1261 tcagcctgtt cggcagcaac taccaggacg tgttcgcgtt cttcgtgctg atcctggtgc
     1321 tgatcttccg cccctctggc ctgttgggcg aacgggtcgc cgatcgcgca tgaacaacgc
     1381 aggtcgcacc ggaataccgc tgcaggtctg ggccggcgtc gcgctggtcg cgatcgcgct
     1441 gggcgtgctg cccttcgtcg ccgcgctggc cggccaggca tgggtccgca ttctcaactt
     1501 cgcgatggtc tacacgatgc ttgcgctcgg gctcaacatc gtcgtcggat tcgccgggct
     1561 cctcgacctg ggctatatcg ctttctatgc ggtcggcgcg tatacctacg cgctgctg
//
LOCUS       DATANS010000404         1512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_229997, whole genome shotgun sequence.
ACCESSION   DATANS010000404 DATANS010000000
VERSION     DATANS010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229997"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /gene="dapE"
                     /locus_tag="VHT22_05885"
     CDS             complement(<1..672)
                     /gene="dapE"
                     /locus_tag="VHT22_05885"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEX3137119.1"
                     /translation="MSIDVVDLAKELIARHSVTPNDAGCIDLLCGYLEPLGFECEKIS
                     RGGVDNLWARRGTASPVVCFAGHTDVVPTGPLDQWHSDPFTPTIRDGVLYGRGASDMK
                     SSLASFVVAIDEFIRQHPDHPGSLAVLFTSDEEGPAVDGTVRVCEALKDRNEKLDYCI
                     IGEPTAVEKLGDMIKNGRRGSLSGVLTVKGVQGHVAYPQQVKNPIHLVAGAIAEMAAT
                     KWDDGN"
     gene            820..>1512
                     /locus_tag="VHT22_05890"
     CDS             820..>1512
                     /locus_tag="VHT22_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3137120.1"
                     /translation="MPGLLVSHQFNTRYGTDFVAAGKRNSVDIELLVLPPDPEARINE
                     AAAARVEIAYFSSDIIPLHTKQFFSATRKSPALKWMQVFNAGVDHPVFASVLERGVRL
                     TTSSGTAAEPIAQTAIAGMLILARNFPRWLAGQREHRWDPMPPADFPRDLRGQTMLVY
                     GLGAIGVEIARLARLLGLKIIGVRRSPARVEHVDEMHTPDQLGELLPRCDWLTIACPL
                     TAETRGMFNAALL"
BASE COUNT          259 a          531 c          460 g          262 t
ORIGIN      
        1 gttgccatcg tcccatttcg tcgcggccat ctcggcgatt gcgccggcca ccaggtggat
       61 cgggttcttc acctgctgcg gatacgcgac gtgcccctgc acgcccttca ccgtcagcac
      121 gccggacaag gagccgcgcc ggccgttctt gatcatgtct cccagcttct cgaccgctgt
      181 cggctcgccg atgatgcagt agtcgagctt ttcgttgcgg tctttcagcg cctcgcatac
      241 gcgcacggtg ccgtcgacgg ccgggccttc ctcgtcggac gtgaacagca cggcaagaga
      301 acccggatgg tcgggatgct ggcggatgaa ttcgtcgatg gcgacgacga acgaagcgag
      361 cgagcttttc atgtccgatg cgccgcgccc gtacagcacg ccgtcgcgaa tggtcggcgt
      421 aaacggatcg ctgtgccact gatcgagcgg cccggtcggc accacgtcgg tgtggcccgc
      481 gaagcacacc accgggctcg ccgtgccgcg ccgcgcccac aggttgtcga cgccacccct
      541 actgattttt tcgcactcga acccgagcgg ctcgagatac ccgcacaaaa ggtcgatgca
      601 gcccgcgtcg ttgggcgtca cggaatggcg ggcaataagt tctttggcga ggtcgacaac
      661 gtcgatggac acgggatggc tttcgcttaa cgcaacgccg catcttagca aatcggcgcg
      721 cgccggcagt gccgattacc cgctaaaata gcgcaaaacc cgcccgtcgc ccgcagtgcc
      781 cgcagacggc gtcccatcga ctgcagagga aatccccgcg tgcccggctt gctcgtctca
      841 catcagttca acacccgcta cggcacggat ttcgtcgccg ccggcaagcg caacagcgtc
      901 gacatcgaac tattggtcct gccgccggac cccgaggcac gcattaacga agccgctgcc
      961 gcgcgcgtgg aaatcgcgta cttcagcagc gatatcattc cgctccatac caagcagttc
     1021 ttctccgcca cgcgcaagtc cccggcgctc aagtggatgc aggtattcaa cgccggcgtc
     1081 gatcacccgg tgttcgcgag cgtgctcgag cgcggcgtgc ggcttacgac atcgtcgggc
     1141 accgcggccg agccgatcgc ccagacggca atcgccggca tgctgatact cgcgcgcaat
     1201 ttcccgcgct ggctcgccgg ccagcgcgag cacagatggg atccgatgcc gcctgcggat
     1261 ttcccacgcg acttgcgcgg ccagaccatg ctcgtctacg gtttgggcgc gatcggtgtc
     1321 gaaatcgcgc ggcttgcgcg cctgctcgga ctcaagatca tcggcgtgcg gcgcagcccg
     1381 gcgcgcgtcg agcacgtcga cgaaatgcat acccccgacc agctcggcga actcctgccg
     1441 cgctgcgact ggctcacgat cgcgtgcccg ctgaccgccg agacgcgcgg catgttcaac
     1501 gccgcgctgc tc
//
LOCUS       DATANS010000405         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_147401, whole genome shotgun sequence.
ACCESSION   DATANS010000405 DATANS010000000
VERSION     DATANS010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147401"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1833
                     /locus_tag="VHT22_05895"
     CDS             <1..1833
                     /locus_tag="VHT22_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3137121.1"
                     /translation="LSLIFGVTRVVNFAHGSLMMLGAYIAYSLIGRFGSGPLGYWAGV
                     LLAALAVGALGVLIERMLLRRLYGAPELLQLIATFGVVLLVKDAALAIWGAEDLLGPR
                     APGLAGAIKIFGQPVPQYDLLLIVLAPLVLAALSLALARTRWGLMVRAATEDRDMTAA
                     LGVNPSWLFSGVFFVGALLAGLAGALQLPREPANLNMDLTIIADVFVVTVVGGLGSIR
                     GAFVAALIIGIVRALCIGIGDVVLFGSLVSFAKLTLVAEFVVMAIVLIARPWGLYGRP
                     PASSPIPEQRDLQLPPGRRFALAAAFLFAALAAAPLIADDYALVLGSDMLIFALFAVS
                     LHFIMGPGGMASFGHAAYFGLGAYAAALAARVVPMELALIAAPLAAGIGALVFGWFCV
                     RLSGVYLAMLTLAFAQITWATVYQWDALTGGSNGLVGVWPAPWLSSRTAYYWLTLAIA
                     GGAIALLWRIVFAPFGYALRAARDSPRRTAAIGIDVRTVRWAGFVLAGLAAGLAGSLY
                     AFSKGSISPESLSIPRSVDALVMVLLGGLQTLLGPVAGAALLTWLQDTLARHTEYWRA
                     MLGAAILALVLLFPQGIVGALAGATRALRQRAAAAAPAPSVGGP"
     gene            1830..>2240
                     /locus_tag="VHT22_05900"
     CDS             1830..>2240
                     /locus_tag="VHT22_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3137122.1"
                     /translation="MTVPLLSVHDIAKAFGGVQALAGVSFDVAAGERVAVIGPNGAGK
                     TTLFNVLDGQLAPDRGSAALAGQALIGLSTQTIARLGVGRTFQVTATFASMTVRENVQ
                     LALLAQRGGTYALLSGLDARGAAEAQRLLEQVGIG"
BASE COUNT          258 a          756 c          781 g          445 t
ORIGIN      
        1 ctttcgctga tcttcggcgt cacccgcgtc gtcaacttcg cgcacggctc gctgatgatg
       61 ctgggtgcgt acatcgccta cagcctgatc ggacgcttcg gttccgggcc gctcggctac
      121 tgggccgggg tgctgctcgc tgcgctggcg gtcggcgcgc tgggcgtgct gatcgagcga
      181 atgctgctgc ggcgcctcta cggtgcgccg gaactgctgc agctgatcgc gaccttcggc
      241 gtcgtcctgc tggtcaagga tgcggcgctg gccatctggg gcgcggagga tctgctgggg
      301 ccgcgtgcgc cgggacttgc cggcgcgatc aagatcttcg gccagccggt gccgcaatac
      361 gaccttctcc tgatcgtgct cgcgccgctc gtgctggccg ccttgtcgct cgcactggcg
      421 cgcacgcgct ggggcctgat ggtgcgtgcg gcgaccgagg atcgggatat gaccgccgcg
      481 ctcggcgtca atccgtcgtg gcttttttca ggcgtgttct tcgtcggcgc gctgcttgcg
      541 ggactcgccg gcgcgctgca gctgccgcgc gagccggcga atctgaatat ggacctgacg
      601 atcatcgccg atgttttcgt cgtaaccgtc gtcggcggcc tcggcagcat tcgcggcgct
      661 ttcgttgccg cgctgatcat cggcatagtc agggcgcttt gcatcggcat tggcgatgtc
      721 gtgctgttcg gctcgctcgt atcgttcgcg aaattgaccc tggtcgcgga attcgtcgtg
      781 atggcgatcg tgctgatcgc tcggccgtgg ggcttgtatg gccgcccgcc agcctcctcc
      841 ccgatccccg agcagcgcga tctgcagttg ccgccgggac gacgcttcgc gctcgccgcg
      901 gcgtttttgt tcgcagcgct cgcggcagcg ccgctcattg ccgacgacta cgcgctggtg
      961 ctcggcagcg acatgctgat attcgcgctg ttcgccgtca gcctgcattt catcatgggt
     1021 cccggcggca tggcctcgtt cgggcacgcc gcgtacttcg gcctcggtgc gtatgccgcg
     1081 gcgctcgctg cacgggtggt gccgatggag ctcgcgctga tcgctgcgcc gctcgcggca
     1141 ggaatcggcg cgctggtctt cggctggttc tgcgtgcgcc tctcgggcgt ctaccttgcg
     1201 atgctgacgc tcgcattcgc ccagatcact tgggccaccg tctaccaatg ggacgcattg
     1261 accggcggca gcaacggtct ggtcggcgtc tggccggctc catggttgtc gtcgcgcacc
     1321 gcctactact ggctgacgct ggcgatcgcc ggcggcgcga tcgccttgtt gtggcggatc
     1381 gtattcgcgc cgttcggtta cgccctgcgt gcggcgcgcg actcgccgcg gcgcaccgcc
     1441 gcgatcggca tcgacgtccg caccgtgcgt tgggccggat tcgtgctcgc gggcctcgcc
     1501 gcagggctgg ccgggtcgct ttacgcattc tccaaaggca gtatctcacc cgaatccttg
     1561 tcgataccgc gctcggtcga cgcactggtg atggtgctgc tcggcgggtt gcagaccttg
     1621 ttaggaccgg tcgccggcgc ggccttactg acttggcttc aggacacgct ggcgcggcac
     1681 accgagtact ggcgcgcgat gctcggcgcg gcgatcctgg ccctggtgct gctctttccg
     1741 caaggcatcg tcggcgcctt ggctggcgcc acacgcgctc tgcggcaacg cgcggcggca
     1801 gcggcaccag ccccaagtgt cgggggccca tgaccgtgcc gctgctttcg gtgcacgaca
     1861 tcgccaaggc ctttggcggc gtgcaggcac tcgccggcgt gagcttcgac gtagcggccg
     1921 gcgagcgcgt cgcggtcatc ggtcccaacg gcgccggcaa aacgaccttg ttcaacgtcc
     1981 tcgacggcca gctcgcgccg gatcgcggca gcgcggcctt ggcggggcag gcattgatcg
     2041 ggctctcgac gcagacgatt gcacggctcg gcgtcggccg cacgttccag gtgacggcga
     2101 cgttcgcctc gatgaccgtc cgggaaaacg tccagctcgc gctgctcgcg cagcgcgggg
     2161 gaacctatgc gctgctgtcg gggcttgacg cgcgcggcgc ggccgaagcg caacggctgc
     2221 tcgagcaggt cggcatcggc
//
LOCUS       DATANS010000406         3921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_651963, whole genome shotgun sequence.
ACCESSION   DATANS010000406 DATANS010000000
VERSION     DATANS010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651963"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..214
                     /locus_tag="VHT22_05905"
                     /pseudo
     CDS             1..214
                     /locus_tag="VHT22_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013754847.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            212..898
                     /gene="pdsR"
                     /locus_tag="VHT22_05910"
     CDS             212..898
                     /gene="pdsR"
                     /locus_tag="VHT22_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018691332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteobacterial dedicated sortase system
                     response regulator"
                     /protein_id="HEX3137123.1"
                     /translation="MAIVEDEPAIRANYADALKKQGYEIATYANRSDALGALRTRLPD
                     LALIDIGLGDEIDGGFALCRELRALSESLPIIFLSARDSDFDVVAGLRLGADDYLTKD
                     VSLPHLSARIAALFRRSDLLAAPHAVEEVIERGPLKLDLKRLTAYWAGSRVDLTLTEF
                     WMVHTLAKHPGHVKDRDSLMRDASIVVDDSTITSHVKRIRRKFAALDPKFDCIATVYG
                     MGYRWNAAEQ"
     gene            895..2550
                     /locus_tag="VHT22_05915"
     CDS             895..2550
                     /locus_tag="VHT22_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009574774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3137124.1"
                     /translation="MSRFRIALGIRAQLLLVLMVFLTIPWLGYEYVRELERFLRDAQQ
                     KTLAGTAQAVATALHDRPRLFETSNAAGESLAAQRALDGAPEAEAAPQLLRASGPAAT
                     PSAEIGQIIQGLSRTSARIWVIDRQLDVLARAGSLKRPPLPETDAPDSAIPRVWRWIE
                     RETLHPLYTLILSQPTDDFVDELAAHVAMPAREVDGALSGILTVDRRLTGDGKAVVVS
                     AAHPIWVGDQVKGAVIVEETTNAVLAERNRAFERLFNIVLAALLIGSVALTLYASRLT
                     ARIRRLRDEAEAAIDLQGRIRGTVSPSAAGDEIGDLSRSFSSVVTRLSQYASYQQNMA
                     SRLSHELRTPVAVVRSSLDNLRAAALPDDARVYMERAQQGLTRLTHILTRMTEATRLE
                     ESLGDAQHERFDLASVVAGCVDGYRAAYAGRAFVLKLPPGPLPVDGAPDLIAQLLDKL
                     VANAVEFGIPGTPVVVALERDDDSVSLRVENEGTPLPAGMHERLFDSMVSIRGEQKSE
                     EPHLGLGLYIVRLIAGFHRGRVSAADRDRGDGVVISVNLPLAA"
     gene            2633..2992
                     /locus_tag="VHT22_05920"
     CDS             2633..2992
                     /locus_tag="VHT22_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEX3137125.1"
                     /translation="MPANLLQHPALVAALLLAASNLFMTFAWYAHLRNMQGRAWWVAA
                     LASWGIALFEYLLQVPANRIGYTVLSLGQLKIMQEVITLTIFVPFAFLYMDQPLKLDY
                     VWAAMCMVGAVYFMFRA"
     gene            2989..>3921
                     /locus_tag="VHT22_05925"
     CDS             2989..>3921
                     /locus_tag="VHT22_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3137126.1"
                     /translation="MTALRDLLASAARNAAAGRLDDALANYRGALRRSPALAEVHYNV
                     GVLLARKGELAGAEKSFDQALQLKPSWAQAYLALGHLYFRQARYADAERSFGRAATHA
                     PESVEALFNLATSRDRQRRWGDALPPLKAARGLAPDNEDIWFALRGHLLLFQREEEAF
                     DDFRRFEANAALSARIVAAGLLSARIAPGIDYEDKYLPLALDWPYQPGDAAYAAVAVA
                     QAQYFDVTREQLLRVYQAHDRVRQAERGSIADFAAPLLPAGAAGRRVRIGYLSADLRS
                     HVMGRLMLEVVRRHDTERFSVHGYSLAVREIEDEV"
BASE COUNT          616 a         1310 c         1317 g          678 t
ORIGIN      
        1 gcgcctgacc gcaaactgga atgcgcaaag ggtcgatctc acgctgaccg agttctggat
       61 ggtgcactcg ctcgcgcgtt tccccggtca cgtcaaggat cgcgatgcgc tgatgcgcga
      121 cgccagcatc gtcgtcgacg acagcacgat cacgtcgcac gtgaagcgaa tccggcgcaa
      181 gttccaggca gtcgatcccg cgttcgaccg catcgccatc gtcgaggatg agcccgctat
      241 ccgcgccaac tacgccgacg cactgaaaaa gcagggctac gagatcgcga cctacgccaa
      301 ccgcagcgac gctctcgggg cgctgcgcac tcggctgccc gaccttgcgc tgatcgacat
      361 cggcctgggc gacgaaatcg acggcggctt cgcgctgtgc cgtgagttgc gcgcgctatc
      421 ggaatcgctg ccgatcattt tcctgtcggc gcgcgattcc gacttcgacg tcgtcgccgg
      481 cctgcggctt ggcgccgacg actacctgac caaggacgtg agcctgccgc atctgtcggc
      541 gcggatcgcc gcgcttttcc ggcgcagcga cctgctcgcc gcgccgcatg cggtcgagga
      601 ggtgatcgag cgcggcccgc tcaagctcga cctcaagcgc ttgaccgcct actgggccgg
      661 tagcagggtc gacctgacgc tgaccgaatt ctggatggtg catacgctgg cgaagcatcc
      721 gggccacgtc aaggaccgcg acagcctgat gcgcgacgcc agcatcgtcg tcgacgacag
      781 cactatcacc tcgcacgtca agcgcatccg gcgcaagttc gccgcgctcg atcccaagtt
      841 cgattgcatc gcgacggtct acggcatggg ctatcgctgg aacgcggccg agcaatgagc
      901 cgctttcgca tcgcgctggg catccgtgcg caactgttgc tggtgctcat ggtgttcctg
      961 acgataccgt ggctcggtta cgagtacgtg cgcgagctcg agcgcttcct ccgcgatgcg
     1021 cagcagaaga cgctagccgg aaccgctcag gcggtggcga cggcgctgca cgaccggccg
     1081 cgcttgttcg agacgtcaaa cgccgctggc gagtcgctgg cggcgcagcg cgcactcgac
     1141 ggtgcgccgg aggccgaagc tgcgccgcag cttttgcgcg cgtccggccc cgcggccacg
     1201 ccgtcggccg aaatcgggca gatcatccag ggcttgtcgc ggacgagcgc gcggatctgg
     1261 gtgatcgatc gccagctcga cgtactggca agggcgggga gcctcaagcg gccgccgctg
     1321 ccggagaccg acgcgccgga ctcggcgatt ccacgcgtgt ggcggtggat cgagcgcgag
     1381 acgctgcatc cgttgtacac gctgatcctg agccagccga ccgacgattt cgtcgacgag
     1441 ctcgcggcgc acgtcgcgat gcccgcgcgt gaggtcgacg gcgcgctgtc gggtatcctg
     1501 accgtcgacc ggcggctgac cggcgacggc aaggcagtgg tcgtctcggc cgcgcacccg
     1561 atctgggtcg gcgatcaggt caagggcgct gtgatcgtag aggaaacgac caacgcggtg
     1621 ttggccgagc gcaaccgcgc gttcgagcgc ctgttcaata tcgtgctggc cgcgctgctc
     1681 atcggatcgg tcgcgctaac cctgtacgcg tcgcgcttga ccgcgcgcat ccggcggctg
     1741 cgcgacgaag cggaggccgc gatcgacctg cagggcagga tccgcggcac cgtctcgcct
     1801 tccgcggcgg gcgatgaaat cggcgatctg tcgcgaagct tttcgagcgt cgtcacgcgg
     1861 ctgtcgcaat acgcgagtta tcagcagaac atggcgagca ggttgtcgca cgaattgcgc
     1921 acgccggtcg cggtagtccg ctcgtcgttg gacaatctgc gcgcggcggc gctgccggac
     1981 gacgcgcgcg tctacatgga gcgcgcgcaa cagggtctga cgcggctgac ccatatcctg
     2041 acgcggatga cagaggcgac gcggctcgag gaaagcctgg gcgacgcgca gcacgagcgc
     2101 ttcgacttgg cttcagtggt cgcagggtgc gtcgatggct atcgcgccgc gtacgccggc
     2161 cgcgcgttcg tgctcaagct cccgcccggt ccgctgccgg tcgacggggc gcccgacctg
     2221 atcgcccagc tgctcgacaa gctggtcgcc aacgcggtcg agttcggcat cccgggaacg
     2281 cctgtcgtcg tcgcgctcga gcgggacgac gacagcgtca gccttcgcgt ggagaacgag
     2341 ggtacgccgc ttccggccgg catgcacgag cgcttgttcg actcgatggt gtcgatccgc
     2401 ggcgagcaga aaagcgaaga gcctcatctc gggctggggc tttatatcgt tcgcctgatt
     2461 gccggatttc accgcggccg ggtcagcgcg gccgatcgtg accgcgggga cggcgtcgtc
     2521 atcagcgtaa acctgccgct ggccgcatag cgctcgcgac gccggcatca gcgcgttctg
     2581 ccgtctcgta catcggcgat aatggcgctt tttcttcgcc cgccggactc tcatgcctgc
     2641 caacctgctg caacatccgg cgctcgtcgc tgctttgctg ctggccgcgt cgaacctctt
     2701 catgaccttc gcctggtatg cgcacctgcg caacatgcag ggccgcgcct ggtgggtcgc
     2761 cgcgctggcg agctggggca tcgcgctatt cgaatacctg ctgcaggtgc cggccaaccg
     2821 gatcggatac acggtgctat cgctggggca gctcaagatc atgcaggaag tcatcacgct
     2881 gacgatcttt gttccgtttg ccttcctgta catggatcag ccgctcaagc tcgactacgt
     2941 ctgggcggcg atgtgcatgg tgggcgcggt ctacttcatg ttccgcgcat gacggcgctg
     3001 cgcgacctgc tggcgagcgc tgcacggaac gcggccgccg gcaggctcga tgatgcgcta
     3061 gccaattatc gcggcgcgct caggcgctcg cctgcgctgg ccgaggtcca ttacaacgtc
     3121 ggcgtcctgc tggcgcgcaa gggcgagctc gccggcgccg aaaaaagctt cgaccaagcg
     3181 ctgcaactca agccctcgtg ggcgcaggcc tatctcgcgc tcggacatct ttatttccgg
     3241 caggcgcgat acgccgacgc cgagcgctcg ttcggccgcg cggccacgca tgcgcccgaa
     3301 tccgtcgagg ccctgttcaa cctggcgacc tcgcgcgacc ggcagcggcg ctggggcgac
     3361 gcgttgccgc cactcaaggc agcgcgcgga ctcgcaccgg acaacgagga catctggttc
     3421 gcactgcgcg gacatctgct gctgttccag cgcgaagagg aagccttcga cgattttcgc
     3481 cgcttcgagg cgaacgcggc gctgtcggcc cgcatcgtcg ccgccgggtt gctgtcggcc
     3541 cgaatcgctc ccggaatcga ttacgaggac aagtatctgc cgctcgcgct cgattggccg
     3601 taccagccgg gcgacgcggc gtatgcagcc gtcgcggtgg cgcaagcgca gtatttcgac
     3661 gttacgcgcg agcaactgtt gcgcgtctac caggcgcacg atcgcgtccg ccaggccgag
     3721 cgcggatcga tcgccgattt tgctgcgccg ctgcttcccg cgggcgctgc cggacggcgg
     3781 gttcgcatcg gctacctgtc ggccgatttg cgctcgcacg tcatgggtcg attgatgctc
     3841 gaggtcgtgc gacggcacga taccgagcga ttctccgtgc atggctattc gctggcagtg
     3901 cgcgagatcg aggacgaggt g
//
LOCUS       DATANS010000407         2279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_273991, whole genome shotgun sequence.
ACCESSION   DATANS010000407 DATANS010000000
VERSION     DATANS010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273991"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..851)
                     /locus_tag="VHT22_05930"
     CDS             complement(60..851)
                     /locus_tag="VHT22_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX3137127.1"
                     /translation="MTALSPLYAIVVACLAGGVLAILAAAGTLALHARWISKLVSFAV
                     GALLGAVFLELLPHALEHGSTGSVMATVLGGLLLFFLLEKLVLWRHSHLDAEALGEDG
                     QPAGAPHAHGDGGRSGLMIVIGNSVHNFCDGIVIAAAFLTDFALGTATALAIVAHAIP
                     QQVGDFAVLLHSGYARRRAFAYNLVTGVATLLGGLAGYAALASMQHVLPTVLAIASAS
                     LLYVAVADLIPSLHRHPAPAATVQQLLLIAIGIGLIAAVHRWLEG"
     gene            complement(887..1645)
                     /gene="pgeF"
                     /locus_tag="VHT22_05935"
     CDS             complement(887..1645)
                     /gene="pgeF"
                     /locus_tag="VHT22_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011707735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX3137128.1"
                     /translation="MRADATLAAQFVSAGVDWIVPDWPAPASVRAFSTTRNGVGGTTV
                     DFGRGGDAAAGALLKSFLPGEPLWLAQTHGTSVASADVSYGCAPEADVAVSRSHGKVC
                     AVLAADCLPVLLADRRGSVVAAAHAGWRGLASGVLETAVGSLQTEPSNVLAWLGPAIG
                     PSAFEVGPDVHAAFCDRDAAAGACFVATSQGKWHADLYGLARLALGRAGVRAAAISGG
                     GRCTYTERASFHSYRRGGPDRVARMATLIWLAPG"
     gene            complement(1635..>2279)
                     /locus_tag="VHT22_05940"
     CDS             complement(1635..>2279)
                     /locus_tag="VHT22_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX3137129.1"
                     /translation="VIDKPAGLVVHPGSGNRDGTLANALLHHAPQLAVVARAGIVHRL
                     DKDTSGLLVVAKTEIAQTDLVRQLQAKTVHREYLALATGDIARGGAIDAPIGRHPVRR
                     TSMAIVASGKAAVTRYEVRERFGDCTLLACRLETGRTHQIRVHLTSLGHPLVGDPTYG
                     KRRGIAFGRQALHAWRLGLIHPSTNKPVQWESPLPSDFASLLATLRRRAASDAR"
BASE COUNT          385 a          776 c          794 g          324 t
ORIGIN      
        1 tgccacggga gccacggacg ccggcctggt cttcgcgcgt ttgggcgtat tcatcgatat
       61 cagccttcga gccaacgatg cacggcggcg atcaatccga tgccgatggc gatcagcagc
      121 agttgctgca ccgtcgccgc cggcgccgga tgccggtgca ggctcgggat caggtccgcc
      181 acggcgacgt aaagcaggct cgcggatgcg atcgcgagca cggtgggcaa gacatgctgc
      241 atggaggcca gcgcggcgta gccggcaagg ccgcccagca gcgtcgcgac ccccgtgacc
      301 aggttgtacg cgaacgcgcg ccgtcgtgcg tacccggagt gcaacagcac cgcgaagtcg
      361 ccgacctgct gcggaatcgc atgcgcgacg atggccagcg cagtcgccgt gccgagcgcg
      421 aagtcggtca ggaacgcggc ggcgatcacg atgccatcgc agaaattgtg cacgctgttg
      481 ccgatcacga tcatcaaacc ggaacgaccg ccgtcgccgt gcgcgtgcgg cgcgccggcg
      541 ggctgcccat cttcgccaag cgcctccgcg tcgaggtgac tatgccgcca caggaccagc
      601 ttttccagca ggaagaacag cagcaggccg ccgagcacgg tcgccatcac gctccccgtc
      661 gacccatgct cgagcgcgtg cggaagcaat tccagaaaca cggcgcccag cagcgcaccg
      721 acggcgaagc tcaccagctt ggatatccag cgcgcgtgca gcgcaagcgt gccagcggcg
      781 gcgagtatcg ccagtacccc tccggcgaga caggcgacga cgatcgcgta aagtggagag
      841 agcgctgtca tcgccaacgg attatacgag agcgcccgtt tcggcttcag ccgggcgcga
      901 gccagatcag cgtcgccatc ctcgcgacgc ggtcgggccc gccgcggcga tacgagtgga
      961 agctcgcgcg ctcggtgtaa gtgcagcggc cgccgccgct gatcgccgcg gcgcggacgc
     1021 cagcgcggcc gagtgcaagt cgcgcgagtc catagagatc ggcatgccat ttgccttgac
     1081 ttgtcgcgac gaagcaagcg cccgcggcgg cgtcgcgatc gcagaatgcc gcgtggacgt
     1141 cggggcccac ttcgaatgcg ctcggcccga tcgccggtcc gagccatgcg agcacgttcg
     1201 agggctcagt ctgcagcgac ccgaccgcgg tttcgagcac gccggaggcg agcccgcgcc
     1261 atcccgcatg cgcggcggcg acgacgctgc cgcgccggtc ggcgaggagc accggcagac
     1321 agtccgccgc cagcaccgca cagactttgc cgtgagagcg cgaaaccgcg acgtcggctt
     1381 cgggtgcgca gccatagctg acgtcggcgc tcgcaaccga cgtgccgtgt gtctgcgcca
     1441 gccacagcgg ctccccgggc aggaagctct tcagtagcgc gcctgccgcg gcatccccgc
     1501 cgcgcccgaa gtcgacggtg gtgccgccga cgccgttgcg cgtggtcgag aatgcacgca
     1561 ccgacgcagg cgccggccag tccggtacga tccagtcgac ccctgcggag acgaactgcg
     1621 ccgcgagcgt tgcgtcagcg cgcatcggag gcggcgcgcc ggcgcaaagt cgcgagcagc
     1681 gaggcgaagt cggatggcag cggcgattcc cactgcacgg gtttgtttgt cgaaggatgg
     1741 atcagaccga ggcgccaggc gtggagggcc tgccgtccga acgcgatgcc gcgcctcttg
     1801 ccatacgtcg gatccccgac gagcggatgt ccgagcgagg tcaggtgaac gcggatctgg
     1861 tgggtgcgtc cggtttcgag ccggcaggcg agcagcgtgc agtcgccgaa tcgttcgcgc
     1921 acttcatagc gcgtgaccgc ggctttcccg ctcgcgacga tcgccatcga tgtgcgccgg
     1981 accggatgac gcccgatcgg cgcatcgatc gcgccgcccc gggcgatatc tcccgtggcg
     2041 agcgccagat actcgcggtg gacggtcttc gcctgcagct ggcgcaccag atcagtttga
     2101 gcgatttcgg ttttggccac gaccagcagt ccgctcgtat ccttgtccag gcgatgtacg
     2161 atgcccgcgc gcgcgacgac ggcgagttgc ggagcgtggt gcagcagtgc gttggcaagc
     2221 gtgccgtcgc gattgccgct gcccgggtga acgaccaggc cggcaggctt gtcgatcac
//
LOCUS       DATANS010000408         1866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_274006, whole genome shotgun sequence.
ACCESSION   DATANS010000408 DATANS010000000
VERSION     DATANS010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274006"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1712
                     /locus_tag="VHT22_05945"
     CDS             93..1712
                     /locus_tag="VHT22_05945"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX3137130.1"
                     /translation="MRVAIAQLNQMVGDLPGNAARILDAVDAGRRGGADLVVTPELSL
                     CGYPPEDLLLRPAFLEACALELGRLAMRIAGPAVVVGYPEMGHGGSRHNAVAIIRDGR
                     IVGRYRKHTLPNYTVFDEQRYFTSGTEPCVFDVAGIRVGVVICEDIWFPGPARGSRDA
                     GAELIVVPNGSPYHTRQQALRREVVTARAVETGCPVVYVNRVGGQDELVFDGASFIVD
                     ASGKVTQQLPAWQETIALAEFDGALPKPVRGDLDTRLEPHVYAALTMGVRDYVEKNRF
                     PGVLLGLSGGIDSALTLAVAVDALGRERVRAVMLPSTFNAAISLEDARTMAGILGVRY
                     DEIPIDGVLQAFLRSLAAEFKGLSEDATEENIQARIRGTLLMAMSNKHGSIVLTTGNK
                     SEMAVGYATLYGDMAGGFAVLKDIDKTLVYRLANYRNSLGRVIPERIITRAPSAELRA
                     NQTDQDSLPPYETLDAILEAYVEENLSPSEIIARGHAPAQVAQVVRLIKFAEYKRRQA
                     AVGIRITPRGFGKDWRYPITSAWADWKTGAD"
BASE COUNT          328 a          588 c          618 g          332 t
ORIGIN      
        1 aagcaccacg tgctcctgcg tcgtgatcag cgtgcggcga cgggtgcgcc gctctcggga
       61 cccgaaccgg tgcgcatggt aaaatttgaa ccatgcgcgt cgcgatcgcc cagctgaacc
      121 agatggtcgg cgatcttccc ggcaacgcgg cgcgcattct cgatgcggtg gatgctggca
      181 ggcgcggcgg cgcggacctg gtggtcacgc ccgaattgtc gctctgtgga tacccgcccg
      241 aagacctgct gctccgcccg gcgtttctcg aagcttgcgc acttgaactc ggacggctcg
      301 cgatgcgcat cgccggcccc gctgtcgtcg tcggatatcc agaaatgggc catggcggct
      361 cgcggcacaa cgccgtggcc atcatccgcg acggcaggat cgtcgggcgt taccgcaaac
      421 atacgctgcc caattacacc gtcttcgacg aacagcgtta tttcacgtcg ggcaccgagc
      481 catgcgtgtt cgacgtggcc ggcatcagag tcggcgtggt gatttgtgag gacatctggt
      541 ttcccgggcc ggcgcgcggt tcgcgcgatg cgggcgcgga actgatcgtc gtgcccaacg
      601 gctcgcccta ccatacccgc caacaggcgt tgcgtcgcga ggttgtcact gcgcgcgcgg
      661 tcgagaccgg ttgtccggtc gtctacgtca accgcgtagg cgggcaggac gagctcgttt
      721 tcgacggcgc ttcctttatc gtcgatgcca gcggcaaggt aacgcaacag ctcccggcat
      781 ggcaggagac gattgcgcta gccgagttcg acggcgcgct gccgaagccc gtgcgaggcg
      841 atctcgacac tcggctcgaa ccgcacgttt acgcggcgct gacgatgggc gtccgcgact
      901 acgtcgagaa gaaccgcttt ccgggcgtgc tgctcgggct ctccggcggc atcgattcgg
      961 cgctgaccct ggcggtggcc gttgacgcgc tcggccgcga gcgcgtgcgg gcggtgatgc
     1021 tgccttcgac gttcaacgcc gcgatcagcc tcgaggatgc ccgcacgatg gcgggcatcc
     1081 tgggcgtgcg ttacgacgag attccgatcg atggtgttct gcaggcgttt ctccggtcgc
     1141 tcgcggccga attcaagggc ctctccgaag acgccaccga agaaaatatc caggcacgca
     1201 tccggggcac actgctgatg gcgatgtcga acaagcacgg ttcgatcgta ttgacgaccg
     1261 gtaacaagtc ggaaatggcc gtcggctacg cgacgctcta cggtgacatg gcaggcggtt
     1321 ttgccgtcct caaggatatc gacaagacgc tggtctatcg gcttgcgaac taccgcaaca
     1381 gcctgggtcg cgtcattccc gagcgcatca tcacgcgcgc cccgtcagct gagctccgcg
     1441 ccaatcagac agaccaggat agtctgcctc cgtacgagac actcgacgcg atactcgagg
     1501 cctacgtcga ggagaatctg agtccatccg agatcattgc gcgggggcat gcgcccgctc
     1561 aagtcgcaca ggtcgtgcgg ctgatcaagt tcgccgagta caagcgacgc caggcggccg
     1621 tcggcattcg catcacgccg cgcggtttcg gcaaggattg gcgctatccg attacatcgg
     1681 catgggccga ttggaagacc ggcgcagact gacggtcggc gttcgcggcg tgccgccgct
     1741 cgctggttgc ggcggccggg ctggtgtaac ctaacggcgg caggcacggc ggcgcgaatt
     1801 cgcggcgaaa caggggcggg gaatgaaaaa ggtcgaagcg atcatcaagc cgttcaagct
     1861 cgacga
//
LOCUS       DATANS010000409         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_817055, whole genome shotgun sequence.
ACCESSION   DATANS010000409 DATANS010000000
VERSION     DATANS010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817055"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VHT22_05950"
     CDS             <1..544
                     /locus_tag="VHT22_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3137131.1"
                     /translation="GFAMLGASIPSFWFGLILMQIFAVALGWFPVAGYGDPGVPLADR
                     LHHLVLPAIVLGVVNSALILRFTRASMLDVLGEDHIRTARAKGLSEASVILRHALRNA
                     LIPVITVLGLTFALLIGGAIVTETVFGLPGVGNLVVSAVLRRDYPVIQGALLVIAGLY
                     VLINLAIDLLYLVVDPRVKY"
     gene            549..1433
                     /locus_tag="VHT22_05955"
     CDS             549..1433
                     /locus_tag="VHT22_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012653518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3137132.1"
                     /translation="MSDAVALPIARAPSRSPSAILIGKLFRRKLVLAGAAILLIVTLL
                     ALFAPWITPYGPMAMKISDRMQSPRLAHWFGTDELGRDVFSRVVFGARYSLMIGAAVV
                     LISVTGGVLLGLTAGFFRRLDGPIMRVVDAMMSFPDILLAIALVAVLGASMTNVILAL
                     AIVYTPRVARVVRASTLVVRELLFIEAARALGVSTWRILFIHILNNIASPILVQATFI
                     FAYAVLAEAGLSFLGVGVPPELPTWGTMIASGQQFAHQAIWLVVFPGVAIVLSALSLQ
                     MVGDGFRDLLDPKLRKAI"
     gene            1436..>2020
                     /locus_tag="VHT22_05960"
     CDS             1436..>2020
                     /locus_tag="VHT22_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016330028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3137133.1"
                     /translation="MANPARESSPLLQVRHLRTHFFTDAGVVKAVEDVSFSLDAGETL
                     AVVGESGSGKSVTSLSIMGLVPNPPGRIVGGEILFRTREGPVVDLAKLPVKALRQLRG
                     RDIAMIFQEPMTSLDPVFTVGDQIAEAAALHLGMNRAAAMKRALHMLELVEIPAAKQR
                     LTEYPHQLSGGMRQRVMIALALSCNPSLLIADEPT"
BASE COUNT          298 a          672 c          630 g          420 t
ORIGIN      
        1 gggattcgcg atgctcggcg caagcattcc gagcttctgg ttcggcctca tcctgatgca
       61 gatcttcgcc gtcgcgctcg gctggtttcc cgttgcgggc tacggcgacc cgggcgtgcc
      121 gctcgccgac cggctccatc acctggtgct gccggcgatc gtcctcggcg tcgtcaactc
      181 ggcgctgatc ctgcgcttca cgcgcgcgag catgctcgac gtcctcggcg aggaccacat
      241 ccgcacggca cgcgccaagg gactctcgga agcgagcgtc atcctgcgac atgcgctgcg
      301 caacgcattg attccagtca tcaccgtgct cggcctcacg ttcgccctgc taatcggcgg
      361 tgcgatcgtg accgaaacgg tcttcggtct gcccggtgtc ggcaatctgg tcgtttccgc
      421 cgtcctgcgc cgtgattatc cggtgatcca gggcgcactc ctggtcatcg ccggactcta
      481 tgtcctgatc aacctggcga tcgaccttct gtacctggtc gtcgatccgc gcgtgaagta
      541 ctgacaccat gagcgacgct gtcgcgctcc ccatcgcgcg agcgccatcg cgcagcccgt
      601 cagcgattct tatcggcaag ctcttccgcc gcaagctcgt cctggcgggt gccgcaatcc
      661 tgctgatcgt cacgctgctc gcgctcttcg cgccctggat cacgccatac ggcccgatgg
      721 cgatgaaaat aagcgaccgt atgcaaagcc cgcgtctcgc gcactggttc ggcaccgacg
      781 agctggggcg cgacgtgttc tcgcgcgtgg tgttcggtgc gcgctactcg ctgatgatag
      841 gcgcggcggt cgtgctgatc tcggtgaccg gtggcgttct gctggggctc accgccggat
      901 tttttcgccg gctcgacgga ccgatcatgc gcgtcgtcga cgccatgatg tcgttcccgg
      961 acatcctgct ggcgatcgcg ctggtcgcag tgctgggcgc atcgatgacc aatgtgattc
     1021 tcgcgcttgc aatcgtatac acgccgcgtg tggctcgcgt cgtgcgagcg tcgacgctgg
     1081 tcgtccgcga acttctgttc atcgaggccg cacgcgccct gggcgtctcg acgtggcgaa
     1141 ttctgtttat ccacatcctg aacaacatcg cctcgccgat cctggtgcag gcgacgttca
     1201 tcttcgccta cgcggtgctc gcagaagccg ggctgtcgtt tcttggcgtc ggcgttcccc
     1261 ccgagctgcc gacgtggggc acgatgatcg ccagcggcca gcagtttgcg catcaggcga
     1321 tctggctcgt cgtgtttccg ggcgtcgcga tcgtgctttc cgcgctatcg ctgcagatgg
     1381 tgggcgacgg cttccgcgac ctgctcgatc cgaagcttcg caaggcgatc tgatcgtggc
     1441 gaatcccgca cgcgagtctt ctccgctgct acaggtcaga catctccgga cgcatttctt
     1501 caccgacgca ggcgtcgtca aggcggtcga ggatgtctcg ttctctctgg acgctggcga
     1561 gacgctcgcc gtcgtcggcg aatccggctc cggcaagtcg gtgaccagcc tctccatcat
     1621 ggggctcgtt ccgaaccctc cggggcgaat cgtcggcggc gagatcctgt tccggacccg
     1681 cgagggcccg gtggtcgatc tcgcgaagct cccagtcaag gcgctgcggc agctccgcgg
     1741 acgcgatatt gcaatgatct ttcaggagcc gatgacaagc ctggaccccg tattcaccgt
     1801 cggcgaccag atcgccgaag ctgccgcatt gcatctcggg atgaatcgtg cggcggcgat
     1861 gaagcgtgcg ctgcacatgc tggaactcgt agaaatcccg gcggcgaaac agcggctgac
     1921 cgaatatccg catcaattgt cgggcgggat gcggcaacgg gtgatgatcg cgctggcgct
     1981 ctcttgcaat ccgtcgctgc tgatcgccga cgaaccgacc
//
LOCUS       DATANS010000410         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_62887, whole genome shotgun sequence.
ACCESSION   DATANS010000410 DATANS010000000
VERSION     DATANS010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62887"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VHT22_05965"
     CDS             complement(<1..355)
                     /locus_tag="VHT22_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEX3137134.1"
                     /translation="MKRVADIERFRANLVDELNGAALYNALAAAEPDANRRDIFLQLA
                     EAETRHARFWRDKLAAAGVTDVSFSPTLRTRIISALARRFGPRFVLPTVAAAEFNDRG
                     KYLSQPDAQSISAEER"
     gene            complement(352..1869)
                     /locus_tag="VHT22_05970"
     CDS             complement(352..1869)
                     /locus_tag="VHT22_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX3137135.1"
                     /translation="MRTALFVSIIVFMQGAHAGSLSADVARQAQQNWPEFLDLLAIPN
                     VADQPADIQRNATFLEQAFAKRGFKVRRIDNPAKRPAVFAELAGASPQARTVLLYAHF
                     DGQPVIPENWSQQDPFRPVVKRRDAQGKWQEVGRQQLQASPLDPELRVFARSASDDKA
                     PIMMLLTAVDLLRAQKKSPAINVKVLLDPEEEMGSPSLAGMIDQDRAAFAADAMVILD
                     GPQHDSGRSTLVFGNRGVTQATLTVFGPRAPLHSGHFGNYAPNPAMRLARLLASMKDD
                     NGRVLVKGYYDGVALSSAERASLKAVGDDEAALRKRIGIAQAEKVGDTYQEALQYPSL
                     NVRGMAAAGVGAKAANIVPSEAVAEIDIRTTPTTDGRKLFELLRRHVEQQGYHLVEQT
                     PTDADRATYDKLASLRLGSVEAAARTPMDAAVGQWAFTALKSPTAPNPAQDPVRIRMM
                     GGTVPTDVLVEALHLPFVLIPTVNGDNNQHAHDENLRVGHFLSGTEIVYSLLTTP"
     gene            1956..>2520
                     /locus_tag="VHT22_05975"
     CDS             1956..>2520
                     /locus_tag="VHT22_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX3137136.1"
                     /translation="MSSSTSTPETGSSPARTSFWSDVAHALKGEQHDYTAESLNRATL
                     LLAVPMVLEMLMESLFAVVDVFWVSRLGRDAVAVIGLTESVMSLVYAVAIGISFAATA
                     IVARRIGEKDPESAARSAAQIIMLGVTISAGLGVLLSCFAPDILRLMGVSPAIVELGA
                     DFARIMLGGNVTVFMIFLINAIFRGAGD"
BASE COUNT          392 a          822 c          834 g          472 t
ORIGIN      
        1 cgcgctcttc cgcggagatc gactgcgcat cgggctgact cagatatttg ccgcgatcgt
       61 tgaactcggc cgcggccacg gtcggcagta cgaagcgcgg cccgaagcgg cgggcgagcg
      121 cgctgatgat gcgcgtgcgc agagtcggcg agaacgacac gtcggtcacg cccgccgcag
      181 cgagcttgtc gcgccagaag cgtgcgtggc gcgtttcagc ttcggcgagc tgcaggaaga
      241 tgtcgcgccg attcgcgtcg ggctcggcgg ccgcgagcgc gttgtacagc gccgcgccgt
      301 tcagctcgtc gacgaggttg gcgcgaaatc gctcgatgtc ggctactcgc ttcatggcgt
      361 cgtgagcaga ctgtagacga tttccgtgcc gctgaggaaa tgaccgacgc gcaggttctc
      421 gtcgtgcgcg tgttgattgt tgtcgccgtt gacggtcggg atcagcacga acggcagatg
      481 cagtgcttcg accagcacgt ctgtcggcac cgtgccgccc atcatccgga tgcgcacggg
      541 gtcctgcgca ggattcggcg cagtcggcga cttcagtgct gtgaatgccc actgcccgac
      601 ggctgcatcc atcggcgtgc gtgccgctgc ttcgaccgag ccaaggcgca agctcgcgag
      661 cttgtcgtag gtcgcgcggt ccgcgtcggt tggcgtctgc tcgacgaggt gataaccctg
      721 ctgttcgacg tgacgcctga gtagctcgaa cagcttgcgg ccgtccgttg tcggggtcgt
      781 gcgaatgtcg atctcggcca ctgcctcgct cggcacgatg ttcgcggcct tcgcgccaac
      841 gccggccgca gccatgccgc gcacgttcaa cgacgggtac tgcaaggctt cctggtaggt
      901 gtcgccaacc ttctcggctt gcgcgatgcc aatccgcttg cgcagcgccg cctcgtcgtc
      961 gccaacagct ttcaggctcg cgcgctctgc ggacgagaga gccacgccgt cgtagtagcc
     1021 cttcaccaga acacgaccgt tgtcgtcctt catcgatgcg agcaatcgcg ccagccgcat
     1081 cgccgggttc ggcgcgtagt tgccgaagtg tccgctatgc agcggcgcgc gcggaccgaa
     1141 cacggtcagc gtcgcttgcg tcacgccgcg attgccgaac accagtgtcg agcgaccgga
     1201 atcgtgctgc gggccatcga gaatcaccat tgcgtcggct gcgaacgccg cgcggtcctg
     1261 gtcgatcatg cccgcgaggc tcggcgagcc catctcctct tcgggatcga gcagcacctt
     1321 gacgttgatc gccggagatt tcttctgtgc gcgcaacagg tcgaccgcgg tcaacagcat
     1381 catgatcggt gccttgtcgt cggacgcgga acgcgcgaac acgcgcagct ccggatcgag
     1441 cggacttgcc tgcagctgct gacgtccgac ctcctgccat ttgccctgcg catcgcgccg
     1501 cttcacgacc ggccggaacg ggtcctgctg cgaccagttc tcgggaatga ctggctgacc
     1561 gtcgaagtgc gcatacaaca agactgtgcg cgcttgcgga ctggcgcctg cgagctcggc
     1621 gaacacggcc ggtcgcttgg caggattgtc gatccgccgc accttgaaac cgcgcttcgc
     1681 gaacgcctgc tcgaggaagg tcgcgttgcg ctggatgtcg gccggttggt ccgcgacgtt
     1741 ggggatcgcg agcaggtcga gaaattccgg ccaattctgc tgcgcctggc gcgcgacgtc
     1801 ggcgctcagc gagcccgcgt gcgcgccctg catgaaaacg atgatcgaca cgaacagtgc
     1861 cgtgcgcatg gattgctccg ctgtggaatg gcgcacgaaa gctagcatag aatgcgcgct
     1921 tttcgcgtac cggctgccgg ccgcagccac acttcatgtc ttcctccacg tcaaccccag
     1981 agaccggctc gtcgccagca cgcacgtcgt tctggagcga cgtcgcgcac gcgttgaaag
     2041 gcgagcagca cgactacacg gcggaatcgc tcaatcgcgc gacgctgctg ctcgccgtgc
     2101 cgatggtcct ggaaatgctg atggagtcgc tgttcgcggt cgtcgacgtc ttctgggtgt
     2161 cgcgcctcgg gcgcgatgcc gtcgccgtga tcgggctgac ggagtcggtg atgagcctgg
     2221 tctacgcagt tgccatcggc atctcgtttg cagctaccgc gatcgtcgca cgtcgcatcg
     2281 gcgagaaaga tccagaaagc gctgcgcgct cggcggcgca gatcatcatg ctcggcgtga
     2341 cgatctccgc ggggctcggc gtgctgctca gctgcttcgc gccggacatc ctgcggctga
     2401 tgggcgtgag ccccgcgatc gtcgagctcg gtgcggactt cgcgcgcatc atgctcggcg
     2461 gcaacgtgac cgtgttcatg atcttcctga tcaacgcgat cttccgcggc gccggcgacg
//
LOCUS       DATANS010000411         3071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_694476, whole genome shotgun sequence.
ACCESSION   DATANS010000411 DATANS010000000
VERSION     DATANS010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694476"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1100
                     /locus_tag="VHT22_05980"
     CDS             174..1100
                     /locus_tag="VHT22_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX3137137.1"
                     /translation="MSHVIAMIQQYGLGFVFLNVLALQAGLPIPAYPTLIVAGAYAAI
                     GGDPLWALVLVGIAAALIADTGWYVAGRRFGLRILNTLCRVSLSQDSCVRQTETIFQR
                     FGPASMLFAKFVPGFASVATALAGALRLRYWVFLVFDAIGAGLWVSVGLGLGYLFRDA
                     ISDLMDKLASLGKYGSMLVAFGFVAWILLKWWRRRLFIKQLRMDRVSVDELRDLIDAN
                     KASAIVDVRSPLTQAATGRIPGALTIDIKNIAKGFDGVAKDDEVIVYCACPNEATAVK
                     VAQQLRKLGFKRIRPLHGGIDAWIGAGNEVEH"
     gene            1272..1706
                     /locus_tag="VHT22_05985"
     CDS             1272..1706
                     /locus_tag="VHT22_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX3137138.1"
                     /translation="MSKQAIHTSKAPTALGPYSQAIRAGYTVYLSGQVGIDPPSGDLV
                     KGIEAQTRQVFANLRAVAEAAGGSLDDIVKLTILMVDLADFAKVNDIMASCFNTPYPA
                     RATYQVAALPKAALIEVEGILELGRIAETTRAGARSQIPDKA"
     gene            1711..>3071
                     /locus_tag="VHT22_05990"
     CDS             1711..>3071
                     /locus_tag="VHT22_05990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX3137139.1"
                     /translation="MARKKAAAKEPRPANTLQDKLSRIGIGREQDLVLHLPLRYEDQT
                     HLCPLAALKPGQAWHVEGVVANTEIQYRGRRQLVCLLDDGAERSAQLVLRFFHFYPNQ
                     QKALAPGKRVRAFGEVRDGHFGLEMVHPSFQVVAPGTPLPDRLTPVYPTTAGLSQDAL
                     RKLVRRALLADPAFLGETLPPWTREPRRLWGFAEAVHFLHEPPPEAANATAQASLDER
                     THPAWTRLKFDELLAQQLSLKMHRRARAKRKAPRLPGDGSLTRELLARLPFKLTQAQQ
                     RVIAEIRRDLARGEPMQRLLQGDVGSGKTIVAALAALQAIESGYQVAFMAPTEILAEQ
                     HYRKLAAWLDGLPLQIAWLSGGLPAKDRKKALAAIVGGEAQLAIGTHALFQEEVVLPK
                     LGLAIVDEQHRFGVAQRLALRQKGIGDAHQLMMSATPIPRTLAMSFYADLDVSVIDQM
                     PPGR"
BASE COUNT          531 a          970 c         1036 g          534 t
ORIGIN      
        1 gggagtaaac cgggacggca ttagcttata accgccgcat cactctcggt gaattgcgac
       61 gtctgaccga cgcctgcgtc atgcgtgaat aggccccata gcttgatccg cgctcggccg
      121 ccaccatgta agctatccgc acgccgccgc gacaagaaca ggcgaggtac ccgatgagcc
      181 atgtcattgc gatgatccag cagtacggtt tggggttcgt gttcctgaac gtactcgcgc
      241 tgcaggccgg attgccgatt cccgcctatc cgacgctgat cgtggccggt gcgtatgcgg
      301 ccatcggcgg cgacccgctc tgggcgctgg tcctcgttgg catcgccgcg gcgctgatcg
      361 cggacactgg gtggtatgtc gccggccgcc gtttcgggtt gcgcattctg aacacgcttt
      421 gtcgcgtgtc gctgtcgcag gattcctgcg ttcgtcagac ggagacgatt ttccagcgct
      481 tcggacccgc gtcgatgctg ttcgccaagt tcgtgccggg cttcgcttcg gtggcgaccg
      541 cactcgccgg cgcgctgcgc ctgcgctatt gggtcttcct ggtgttcgac gcgatagggg
      601 cggggctttg ggtaagcgtc ggtctcggcc tgggctacct gttccgtgac gcgatcagcg
      661 acttgatgga caagctggct tcgctgggta aatacggctc gatgctggtt gccttcggct
      721 tcgtcgcctg gatcctgctc aagtggtggc ggcgccggct gttcatcaag cagctgcgca
      781 tggaccgcgt gtcggtcgat gagctccgtg atctgatcga tgcgaacaag gcgagcgcaa
      841 tcgtcgatgt ccgctcaccg ctgacgcagg cggcgaccgg acgcataccc ggtgcgctga
      901 cgatagacat caagaacatc gccaaaggct tcgacggcgt ggccaaggac gacgaagtga
      961 tcgtgtattg cgcctgcccg aacgaggcga ccgcagtcaa ggtcgcgcag cagctgagaa
     1021 agctcgggtt caagcggatt cgtccgttgc acgggggcat cgacgcctgg atcggcgccg
     1081 gcaacgaagt cgagcattag atccgacgcc tcggcacacc ggtcacgcgc gtgctagatt
     1141 tgcgcttcga cgagcgcgag agggcatgag cgaggcggga gcttcaagac ccgacgggcc
     1201 gtcgccgagg gattgcccgg aggccgacgc gtgagcgcgg gccgctcgcg gcatcgcaag
     1261 gaggccgagg catgagcaag caagccatcc acaccagcaa ggctccgacc gcgctgggtc
     1321 cgtattcgca ggcgattcgc gcaggctaca ccgtctacct ttcgggccag gtcggtatcg
     1381 atcctcccag cggcgatctg gtgaaaggca tcgaagcgca gacgcggcag gtgttcgcca
     1441 atctgcgcgc ggtcgccgaa gctgccggcg gttcgctcga cgatatcgtc aagctgacga
     1501 tactgatggt cgacctcgcc gacttcgcca aggtgaacga catcatggcg agttgcttca
     1561 acacgccata ccctgcccgc gcgacctacc aggtggcggc attgcccaaa gcggcgctga
     1621 tcgaggtcga gggcatcctc gagctgggac gcatcgcgga aacgacgcgg gcgggagcgc
     1681 gctcgcagat tccggacaaa gcctgaacga gtggccagga aaaaggcggc tgcgaaagag
     1741 ccccggccgg caaacacgct gcaggacaag ctctcgcgca taggcatcgg acgggagcag
     1801 gatctggtgc tgcacctgcc gctgcgctac gaggatcaga cccatttgtg tccgctggcc
     1861 gcgctgaagc ctggacaggc gtggcacgtc gagggcgtcg tcgccaacac cgagattcaa
     1921 tatcgcgggc ggcgccagct cgtctgtctg ctcgacgacg gcgcggagcg cagtgcgcaa
     1981 ctggtgctcc gtttttttca tttctacccg aatcagcaga aggcgcttgc gccgggaaag
     2041 cgcgtgcgcg cgttcggcga agttcgcgat ggacacttcg gactggaaat ggtccacccg
     2101 agcttccagg tggtcgcacc cggaactccg ttgccggacc ggctgacacc ggtctacccg
     2161 acgacagcgg gactctcgca ggatgcgttg cgcaagctcg tgcgtcgcgc gctgctcgcc
     2221 gatcccgcgt ttctgggcga gacattgccc ccgtggacgc gagagccgcg caggctctgg
     2281 ggcttcgcgg aggcggtgca ttttctgcac gagccgccgc cggaagcagc caacgcgacc
     2341 gcgcaggcga gcctcgatga acgcacgcat ccggcgtgga cccggctcaa gttcgatgag
     2401 ctgctggcgc aacagctgtc gctgaagatg caccgccgcg cccgcgccaa gcgcaaggcg
     2461 ccgcgattgc ccggcgatgg ttcactgacg cgcgagctgc tcgcgcgcct gccgttcaag
     2521 ctgacgcagg cgcagcagcg tgttatcgcc gagatccgcc gcgatctcgc gcgcggcgaa
     2581 ccgatgcagc ggctgcttca gggggacgtc ggcagcggca agacgatcgt cgccgcgctg
     2641 gccgcgctgc aggcgatcga atccggttat caggtggcgt tcatggcgcc gaccgaaatt
     2701 ctggccgagc agcattaccg caagctcgcg gcctggctcg acggcttgcc gctgcaaatc
     2761 gcctggttgt ccggcgggct gcctgcgaaa gaccgcaaaa aggcgcttgc ggccatcgtc
     2821 ggcggcgagg cgcagctcgc gatcggtacg catgcgctgt ttcaggaaga ggtcgtgctc
     2881 ccgaagctgg ggctggccat cgtcgacgag cagcatcgtt tcggggtcgc gcaaagactc
     2941 gcgcttcggc agaaaggcat cggcgacgcg caccagctga tgatgagcgc gaccccgatc
     3001 ccgcgcacct tggcgatgag tttctacgcc gatctcgacg tgtcggtgat cgaccagatg
     3061 ccgcccggcc g
//
LOCUS       DATANS010000412         1747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_567195, whole genome shotgun sequence.
ACCESSION   DATANS010000412 DATANS010000000
VERSION     DATANS010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567195"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VHT22_05995"
     CDS             <1..551
                     /locus_tag="VHT22_05995"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX3137140.1"
                     /translation="TVDQLTRYLRQGRDENHGTAAGPMSPVVHGLSEVPEADIRAMAT
                     YLTTVMAGAPAGSGRRAALSLDDAQVSSGAAIFAGTCASCHGEGASSLLSVHTVPLAL
                     TTSIHEPDPRNVLHIVLEGIWPEPGEKGEQMPGFEGAFTDEQLTSLLAYVRVHFANSP
                     PWVDVSRQARDIISTRMKTSDD"
     gene            544..1035
                     /locus_tag="VHT22_06000"
     CDS             544..1035
                     /locus_tag="VHT22_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014530734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX3137141.1"
                     /translation="MIELNVNGKTQSVDAAPDTPLLYVLRDHLAMNGAKFGCGLGQCG
                     ACTVMVDGDAVFSCLTPISVLAGRKIKTVEGLGTIENPGPLQRAFIDEQAAQCGYCIA
                     GMIMRAQALLEKNPSPSDAEIRRHMMPNLCRCGTQMRIFRAVRRAAQAMQAGGAAPDR
                     GRA"
     gene            1032..>1747
                     /locus_tag="VHT22_06005"
     CDS             1032..>1747
                     /locus_tag="VHT22_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX3137142.1"
                     /translation="MNPFDSGRHALSRREFLAASGALIIGFSLASSSLRAQKPPGPKL
                     PGSLDANPMLDSWIRIDADGSISVFTGKAELGQGVKTALIQIAAEQLYVPPQRIRLIT
                     ADTRQTPDEGYTAGSNSMKDSGTAIMNAAAQVREILIARASERLAVAADRLKAQDGAV
                     VTDDGRRLGFGELVGDSLTRVRADGQSKLKDPATYSVVGKAMPRVDIPAKVTGGVAYV
                     QDLRLPGMVHARVVRPPTLDS"
BASE COUNT          309 a          554 c          586 g          298 t
ORIGIN      
        1 ggacggtcga ccagttgact cggtaccttc gccagggtcg agacgaaaac catggcaccg
       61 ccgccggccc gatgtcgccg gtggttcatg ggttgtcgga ggtgcccgag gcagacatac
      121 gcgcgatggc gacctatctg acgacggtca tggcaggcgc tccggcggga tcgggccgcc
      181 gcgccgcttt gagcctcgac gacgcccagg tctcgagcgg cgcggcgatt ttcgccggca
      241 cctgcgcgag ctgtcacggt gagggcgcaa gctcgctatt atccgtgcat accgtcccgc
      301 ttgcattgac gacgtcgatc catgagcccg atccgcgcaa tgtcctgcat atcgtgctcg
      361 agggcatctg gccggagccc ggtgaaaagg gcgagcagat gccgggcttc gaaggcgcgt
      421 tcaccgatga acaactcacg tcgctgctgg catatgttcg cgtgcacttc gccaacagtc
      481 cgccatgggt cgatgtttcc cggcaggcac gagacatcat cagcacaagg atgaaaacga
      541 gcgatgattg agctcaacgt caacggcaag acgcagagcg tcgacgccgc cccggatacg
      601 ccactgctct atgtgctgcg cgatcatctg gcgatgaacg gcgccaagtt cggctgcggt
      661 ctgggtcagt gcggcgcctg cacggtgatg gtcgacggcg atgcggtgtt ttcctgcctg
      721 acgccgattt cagtgctggc cggacgcaag atcaagaccg tcgagggact gggcacgatc
      781 gagaatcccg gcccgctgca gcgtgccttc attgacgagc aggcggcgca atgcggttat
      841 tgcatcgccg gaatgatcat gcgcgcccag gcgctgctgg agaagaatcc ttcgccgtcg
      901 gatgccgaga ttcgccgcca catgatgccc aacctttgtc gctgcggaac acagatgcgt
      961 atctttcgcg cggttcgccg agcggcgcag gcgatgcagg cggggggcgc tgcccccgac
     1021 cggggccgcg catgaatccg ttcgattcgg ggcgccacgc gctgtcgcgg cgcgaatttc
     1081 tcgccgcaag cggcgcgctg atcatcggct tttcactggc gtcgtcgtcg ctgcgtgcgc
     1141 aaaaaccgcc gggcccgaag cttcccggca gtctcgacgc caatccgatg ctggactcgt
     1201 ggatccgcat cgatgccgat ggcagcatta gcgtgttcac cggcaaggcc gagctggggc
     1261 agggcgtcaa gaccgcgttg atccagatcg ctgccgagca attgtatgtg ccgccgcagc
     1321 ggatccgtct gatcaccgcc gacacgcggc aaacgccgga cgaaggttac acggcgggca
     1381 gcaactcgat gaaggacagc ggcacggcga tcatgaacgc ggccgcgcag gtgcgcgaaa
     1441 tcctgattgc tcgagcgtcc gagcgtctcg ctgtggctgc cgatcgactc aaggcgcagg
     1501 acggcgccgt cgtcaccgat gacggacggc ggctcggatt cggcgagctc gtcggcgaca
     1561 gtctgaccag ggtacgcgcc gacggacaat cgaagctcaa ggacccggcg acctattcgg
     1621 tcgtcggcaa ggcgatgccg cgcgtcgata tccctgccaa ggtgaccggc ggcgtggcct
     1681 atgtgcagga cctccgattg cccggcatgg tgcacgcgcg cgtcgtgcgg ccgccgacgc
     1741 tcgactc
//
LOCUS       DATANS010000413         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_776788, whole genome shotgun sequence.
ACCESSION   DATANS010000413 DATANS010000000
VERSION     DATANS010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776788"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VHT22_06010"
     CDS             <1..737
                     /locus_tag="VHT22_06010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3137143.1"
                     /translation="RWLYTKATAEIVTTGEAIRTQLIRDIGVAPSRVSSIPTGIDPVR
                     FGPANRDDARRSLGLPAGVPLIGIVATLRSWKGHRILIEAMTLLAHREAQLVIVGDGP
                     QRDALRAQIGALGLDARIRLAGNQNDVAPWFAALDVFALPSYANEGVPQALLQAMLMA
                     LPCVTTDAGAIGEAAIPDHTAVVVAKENAVALAAGIDRLLGDTALARRIAHAARERAL
                     SRFSLAAMLDRMEAVFQRAVDAHDDE"
     gene            724..1848
                     /locus_tag="VHT22_06015"
     CDS             724..1848
                     /locus_tag="VHT22_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX3137144.1"
                     /translation="MTTSSRGHRLAGRAKVVSRALARRALTLGRRSAPREPRRILIAH
                     HLLLGDTLMLTSLLAKLRGRHPAADIVMALPEPYAALYQPAPYGVRAIGWNPRVPSRS
                     PLWREREFDLAFVPGDNRFSWLAQALGSRWIVGFDGDRPARKSWLFDELRRYPDEPAA
                     WTDMVAALADGPPPAPYARRDWRDPAADPFELPRGRYAVLHVGASTPLKRWEPDKWRA
                     LAAWLAARGVEPVWSAGRGEEAIVRECDPGRRHLSYAGALTLAQLWRLLADAALLVAP
                     DTGIAHLGRVVAVPTVALFGPGSALISGAGLFWRDAPFRAVTVDPFACRDQRVLFRRE
                     IAWVRRCGRGIAECPRPRCMEAIDLAQSCAAIVDLGVVDA"
     gene            complement(1874..>2167)
                     /locus_tag="VHT22_06020"
     CDS             complement(1874..>2167)
                     /locus_tag="VHT22_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEX3137145.1"
                     /translation="IRVVFTGLRPGEKLYEEPLASGETTLPTPHPKLRIAQALPGHRE
                     AVEQMVSWLERDRATGDAEVRAWLKTWIPEYALPGEVPVQLAGAVPLFARRRG"
BASE COUNT          291 a          767 c          766 g          343 t
ORIGIN      
        1 cacgatggct gtacaccaag gcgaccgccg agatcgtcac caccggcgaa gcgatacgca
       61 cgcagctgat ccgcgacatc ggggtggcgc cttcgcgcgt cagctcgatt ccgaccggca
      121 tcgatccggt ccgcttcggc ccggcgaacc gcgacgacgc acggcgctcg ctcgggctgc
      181 ccgccggcgt gccgttgatc ggcatcgtcg caaccttgcg cagctggaaa gggcatcgca
      241 tccttatcga ggcgatgact ttgctcgcgc atcgcgaggc gcagctggtg atcgtcggcg
      301 acgggccgca gcgcgacgcg cttcgcgcgc agatcggcgc gcttggcctg gacgcgcgca
      361 ttcggctcgc cggcaaccag aacgatgtcg cgccctggtt cgcggcgctc gacgtgttcg
      421 cgcttccttc gtacgcgaac gaaggcgtgc cgcaggcgct gttgcaggcg atgctgatgg
      481 cgctgccctg cgtgactacc gacgcggggg cgatcggcga ggcggcaatc cccgatcaca
      541 cggcggtcgt cgtggccaag gagaacgcgg tcgcgctcgc cgcgggtata gaccgcctgc
      601 tcggcgacac tgcactcgcg cggcgcatcg cccacgccgc cagggagcgc gccctgtcca
      661 ggttcagcct cgccgcgatg ctcgatcgca tggaggcagt gtttcagcgc gccgtcgacg
      721 ctcatgacga cgagtagccg cggccatcgc ttggccggac gcgccaaagt cgtcagtcgc
      781 gcgctggccc ggcgtgcctt gacgctgggc cggcgcagcg cgccgcgcga gccgcgacgg
      841 atcctgatcg cgcaccatct gctgctcggc gatacgctga tgctcacctc gctgctggct
      901 aagctccgcg gccgacatcc ggcagccgat atcgtgatgg cactgccgga gccttacgcc
      961 gcgctctacc agccggcccc ttacggcgtg cgggccatcg gctggaatcc gcgcgtgccc
     1021 tcgcgttcgc cgctgtggcg cgaacgcgaa ttcgatctgg ccttcgttcc cggcgacaac
     1081 cgcttcagtt ggctagccca ggcactcggc agccgctgga tcgtcggctt cgacggcgac
     1141 cggccagcgc gcaagagctg gctgttcgac gagctgcgcc gctatccgga cgagcccgcg
     1201 gcgtggaccg acatggtcgc ggcgctggct gacgggccgc caccggcgcc gtacgctagg
     1261 cgcgattggc gcgatccggc cgccgatccg ttcgaattgc cgcgcggccg ctacgcggtg
     1321 ctgcacgtag gcgcgagcac gccgctcaag cgctgggagc cggacaagtg gcgtgcgctc
     1381 gcggcatggc tcgcggcgcg cggcgtcgaa ccggtctggt cggcgggtcg cggcgaggag
     1441 gcgatcgtgc gcgaatgcga tcccggcagg cgccacctct cgtacgccgg tgcgctgacc
     1501 ttggcgcaac tgtggcggct gctcgccgac gccgcgctgc tggtcgcgcc cgataccggc
     1561 atcgcccacc ttggacgcgt cgttgcggtc ccgacggtcg cgctgttcgg tccgggctcg
     1621 gcgttgatca gcggcgccgg ccttttctgg cgcgacgcgc cgtttcgcgc ggtgaccgtc
     1681 gatccgtttg cctgccgcga tcagcgagtg ctgttcaggc gcgagatcgc ctgggtgcgc
     1741 cgctgcggcc gcggaatcgc cgagtgcccg cgcccgcgct gcatggaggc gatcgatctg
     1801 gcgcaaagct gcgccgcgat cgtcgatctg ggcgtcgtgg acgcgtaatc gggtctgatc
     1861 gaaagcgcag cgatcagccg cggcgccgcg cgaacagcgg cacggcgcca gccaattgga
     1921 ccggcacctc gcccggcagc gcgtattccg gaatccacgt cttgagccac gcgcggacct
     1981 cggcgtcgcc ggtggcacgg tcgcgctcga gccagctgac catctgctcg acggcttcgc
     2041 gatgtccggg aagcgcctgc gcgatccgca atttcggatg cggcgtcggc aacgtggttt
     2101 cgccggaggc cagcggttcc tcgtagagct tttcgcccgg ccgcagcccg gtgaaaacga
     2161 cgcggat
//
LOCUS       DATANS010000414         1716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_736593, whole genome shotgun sequence.
ACCESSION   DATANS010000414 DATANS010000000
VERSION     DATANS010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736593"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /gene="rmuC"
                     /locus_tag="VHT22_06025"
     CDS             <1..1054
                     /gene="rmuC"
                     /locus_tag="VHT22_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX3137146.1"
                     /translation="IRADELEERLSEMLRAQSESTGRVDAMAQALVGRQSEMTRAVNE
                     RLDSVTHRVGQSMEATTRHTMDSLRVLHERLGIIDNAHKNLTDLTSQVTTLRDVLANK
                     QSRGAFGQARMEAIVQDGMPQGAYEFQYTLSTGKRPDCVVFLPDQRPLCIDAKFPLEA
                     MTALHDARSDEEKKFATQRLRADVMRHVSDIAEKYLISGETQDTALMFVPSESVYAEI
                     HDGFDDVIQKAYRARVVLVSPSLLMLAIQVMQQILKDARMRDAADQIRTEVLNLGDDL
                     GRLRERVLKLQKHFGDVNEDVRQVLISADKIEKRAGRIEELDFSKTDAPVEVPRFVKS
                     ETPELFPVPRKLQAGE"
     gene            1199..>1716
                     /locus_tag="VHT22_06030"
     CDS             1199..>1716
                     /locus_tag="VHT22_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3137147.1"
                     /translation="MTAPEATASPEKSPAPSWREAWAVYLQPRVLIVLMLGFSSGLPL
                     ALSGSTLLVWMRESGVDLGTIGLFALVGTPYTLKFIWAPLVDALHVPVFTRAFGRRRG
                     WLVFSQLLLIVAILLLALTDPTRSPLFVALGALLVATMSSTQDIVVDAFRVESLPESE
                     QAAGMASYVAAYR"
BASE COUNT          295 a          563 c          567 g          291 t
ORIGIN      
        1 gattcgggcc gacgagctgg aggagcgcct gagcgagatg ctgcgggcgc agagcgagtc
       61 caccggccgg gtcgacgcca tggcgcaggc gctggtgggc cgccagtccg agatgacgcg
      121 cgcggtcaat gagcggctgg attcggtgac gcaccgcgtc ggccagtcga tggaagcgac
      181 cacgcgccac accatggaca gtctgcgcgt gctgcatgag cggctcggca tcatcgacaa
      241 cgcgcacaag aacctcaccg acctgacgtc gcaggtgacg accttgcgcg acgtgctcgc
      301 caacaagcag tcgcgcggtg cgttcggcca ggcgcggatg gaagcgatcg tgcaggacgg
      361 catgccgcag ggcgcttacg aattccagta cacgctctcg accggcaagc ggcccgattg
      421 cgtggtgttc ctgcccgacc agcgcccgct gtgcatcgac gcgaaattcc cgctggaggc
      481 gatgaccgcg cttcacgacg cgcgcagcga tgaggaaaag aaattcgcca cccagcggct
      541 gcgcgccgac gtgatgaggc atgtcagcga catcgccgag aaatacctga tatcaggcga
      601 gacccaggac accgcgctga tgttcgtgcc gtcggaatcg gtctatgccg aaatccacga
      661 cggctttgac gacgtgatcc agaaggccta ccgcgcccgc gtcgtgctgg tgtcgccttc
      721 gctgctgatg ctggcgatcc aggtgatgca gcagatcttg aaagacgcgc ggatgcgcga
      781 cgccgccgac cagatccgca ccgaagtgct caacctcggc gacgacctcg gccgcctgcg
      841 cgagcgcgtg ctcaagctgc agaagcactt tggcgacgtc aacgaggatg tgcggcaggt
      901 cctgatctcc gccgacaaga tcgagaagcg cgccgggcgg atcgaggaac tcgatttcag
      961 caagacggat gcgccggtgg aagtgccccg cttcgtcaag agcgaaacgc ccgagctgtt
     1021 cccggtgccg cgcaagctgc aggcggggga gtaggggcaa aacggtagga tgggtagagc
     1081 gcagcgaaac ccgtcaatcg cgcccgctgc tgccgtgatg ggtttcgctg cgctctaccc
     1141 atcctacgat tgaggtcaag ccgtcttctt gtgcccgaat ttctgctaac accgctccat
     1201 gaccgcaccc gaagccaccg cctcgcccga gaaatccccc gcgccctcct ggcgcgaggc
     1261 ttgggccgtg tatctgcagc cgcgcgtgct gatcgtgctg atgctcggct tttcgtccgg
     1321 gctgccgctg gcactgtcgg gatcgacgct cctggtatgg atgcgcgaat ccggcgtcga
     1381 tctcggcacc atcggactgt tcgcgctggt cggcacgccc tacacgctga aattcatctg
     1441 ggcgccgctg gtcgacgcgc tgcatgtgcc ggtcttcacg cgcgccttcg gccgccgccg
     1501 cggctggctg gtgttctcgc aactgctgct gatcgtcgcg atcctcctgc tggcgctgac
     1561 cgatccgaca cgctcgccgc tgttcgtggc gctcggtgcg ctgctggtcg ccacgatgtc
     1621 gtcgacccag gacatcgtgg tcgatgcgtt tcgcgtcgaa agcctgcccg agagcgagca
     1681 ggccgccggc atggcgtcct atgtcgcggc ctaccg
//
LOCUS       DATANS010000415         1905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_20645, whole genome shotgun sequence.
ACCESSION   DATANS010000415 DATANS010000000
VERSION     DATANS010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20645"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VHT22_06035"
     CDS             complement(<1..754)
                     /locus_tag="VHT22_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HEX3137148.1"
                     /translation="MIIGIPRETRVGETRVAATPETVKKLAASGKHEIVVESGAGVAS
                     SITDADYQAAGARIADTAAALGADIVLKVRGPAADELPRLKRGALLIGLLDPFDTAAI
                     ASYAQAGVSGYALEWLPRISRAQSMDVLSSQANIAGYKAVIIAADEYGRFMPMLMTAA
                     GTVKAARVLILGVGVAGLQAIATAKRLGAVIEASDVRPSVKDQVESLGAKWVDVPYAN
                     DEERKIAEGVGGYARPMPQSWLDRQKVEVAKRI"
     gene            complement(842..>1905)
                     /locus_tag="VHT22_06040"
     CDS             complement(842..>1905)
                     /locus_tag="VHT22_06040"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEX3137149.1"
                     /translation="FHRIAVIPGDGIGKEVVPEGLRALEAAAAKFGIAFEFDEFPWSC
                     DYYAKHGRMMPEDWIAQIGGHDAIFFGAVGWPATVPDHVSLWGSLIQWRRHFDQYVNL
                     RPCRLMPGIPTPLAGRRSGDIDFVVVRENTEGEYSSVGGRMFEGTDRETVFQQTVFTR
                     KGVDRIMRFAFELALTRPAKHVTSATKSNGISITMPYWDERFKAMAAQYPAIRTDQYH
                     IDILSAQFVLHPDWFDVVVGSNLFGDILSDLGPAVCGTIGIAPSANINPERNFPSTFE
                     PVHGSAPDIAGKGIADPIGQIWSGALMLEHLGHADAAAAIVRAIEATLADRSAPRTPD
                     LGGKATTVELGKAIAAAIS"
BASE COUNT          309 a          630 c          635 g          331 t
ORIGIN      
        1 cgatgcgctt ggcgacctcg accttctggc ggtcgagcca gctctgcggc atcggccgcg
       61 cgtagccacc gacgccttcg gcgatctttc gctcctcgtc gtttgcatac ggcacatcga
      121 cccacttggc gcccagcgac tcgacctggt ccttgaccga ggggcgaacg tcggacgcct
      181 cgatcacggc gccgaggcgc ttggcggtgg cgatcgcctg caagccggcc acgccgacgc
      241 ccaggatcag cacgcgcgcc gccttcaccg tcccggcggc ggtcatcagc atcggcatga
      301 agcgcccgta ttcgtcggcg gcgatgatca ccgccttgta gccggcgata ttcgcctgcg
      361 aggacagcac gtccattgac tgcgcgcgcg agattcgcgg cagccactcg agcgcgtagc
      421 cgctgacgcc ggcctgcgcg tacgaggcga tcgcggcggt gtcgaacgga tcgagcaggc
      481 cgatcagcag cgcgccccgc ttgagccgcg gcagctcgtc ggcggccggt ccgcgaacct
      541 ttagcacgat gtcggcgccg agcgccgcgg cggtatcggc gatgcgggcg ccggcggctt
      601 gataatcggc atcggtaatg ctcgaagcta caccggcacc ggactcgacg acgatctcgt
      661 gcttgccgct ggccgcgagc ttcttgactg tctcaggcgt tgccgcgacg cgcgtctcgc
      721 cgacgcgcgt ttcgcgcgga atgccgataa tcatggtgat cgtccccttg cggcgatgct
      781 cgttcttgtc cgctatcttg ccacagcgga ccggccttta gcgaaggcgc aactcagtgg
      841 cctaggaaat cgccgcggcg atcgccttgc cgagctccac cgtcgtcgcc ttgccgccga
      901 ggtcgggtgt gcgcggcgcc gatcgatcgg ccagcgtcgc ctcgatcgcg cgcacgatcg
      961 ccgccgccgc atcggcatgg cccagatgct cgagcatcag agcacccgac cagatctgcc
     1021 cgatcggatc ggcgatgccc ttgccggcga tgtccggcgc agagccgtgg accggctcga
     1081 acgtcgatgg aaaattgcgc tcgggattga tgttggccga tggcgcgatc ccgatcgtgc
     1141 cacatacggc ggggcccaga tcggacagga tgtcgccgaa caggttcgag ccgacgacga
     1201 cgtcgaacca gtcggggtgc aagacgaatt gcgcgctcag gatgtcgatg tggtactggt
     1261 cggtgcggat cgccgggtat tgcgcggcca tcgccttgaa ccgctcgtcc cagtagggca
     1321 tcgtgatcga gatgccgttg gacttggttg ccgaggtcac gtgcttcgcc gggcgcgtca
     1381 acgcaagctc gaacgcgaat cgcatgatgc gatcaacgcc cttgcgggtg aaaaccgtct
     1441 gctggaacac cgtctcgcgg tcggttcctt cgaacatgcg gccgccgacc gacgagtatt
     1501 cgccttcggt gttctcgcgt acgacgacga aatcgatgtc gccggaacgg cgcccggcga
     1561 gcggcgtcgg gatgccgggc atcagccggc acggccgcag gttgacgtac tggtcgaaat
     1621 ggcgccgcca ttgaatcagc gagccccaga gcgagacgtg gtcgggaacc gtcgcgggcc
     1681 agccgacggc gccgaagaat atcgcgtcgt ggccgccgat ctgtgcgatc cagtcttcag
     1741 gcatcatgcg tccgtgcttg gcgtagtagt cgcagctcca tggaaattcg tcgaattcga
     1801 atgcgatgcc gaacttcgcc gccgcggctt cgagcgcgcg caagccttcc ggaacgactt
     1861 ccttgccgat gccgtcgccg ggaatcacgg caatgcgatg aaagg
//
LOCUS       DATANS010000416         2998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_442588, whole genome shotgun sequence.
ACCESSION   DATANS010000416 DATANS010000000
VERSION     DATANS010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442588"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="VHT22_06045"
     CDS             complement(<1..1192)
                     /locus_tag="VHT22_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3137150.1"
                     /translation="MLKTKRARMTLIAACAVLLVAAVVWRSSFHVTKAADATKAGPPA
                     VPVVTTTVKEEDVPTYLSGIGTVMPLYAVTVKARVDGQLDKVMFSEGQDVTAGTILAQ
                     IDPRPFQAQLELAQAQKARDSAQLKNSITDLERYTTLWKQDSIAQQTLTTQQATVDQL
                     KATVQADQATIDNAKVQLDYTTIRAPISGRTGVRLVDPGNIVRASDNTGIVSINQINP
                     IAVLFTLPEDRFQDVNRAMKEHGRTALEVRAFAREDNTLLGTGKLILVNNQIDTATGT
                     VQLKAQFPNNAHKLWPGQYVNVHMMMGTRQRAATVPAGTVQRGPNGLFAYVVNADDTV
                     AVRALRVGPIKDGTAIIEEGLEAGTRVVTEGQYKLKPGVKIVEAKAAEAAPGKGKGVP
                     KDKAG"
     gene            complement(1331..2299)
                     /locus_tag="VHT22_06050"
     CDS             complement(1331..2299)
                     /locus_tag="VHT22_06050"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX3137151.1"
                     /translation="MRPTCGKLSAAALLACASVCQAQDYPSRPIRIIVPSTAGGSVDT
                     LARTVGSNLSQRWGQQVVVDNRSGAGGVIGGEITAKAPPDGYTLIMATIAAMATNVSL
                     AKKLPYDPVRDFAPITLVASQQIVLLVNPAVAARTVQELIQLAKAKPGQLTFASAGNG
                     SGGHLSGELLKILAGIDLTHVPYKGISPAIVDVISGQVTMTFASIISGTPHVKTGRLR
                     ALAVTGARRSAALPELPTMMEAGVRGYESSTWYGLLAPKATARAIVTKLNREVVAIIN
                     LPEVKANLLAEGAEPVGNTPEQFGEFIKAEIAKWGKVIRTAGLRAE"
     gene            complement(2319..>2998)
                     /locus_tag="VHT22_06055"
     CDS             complement(2319..>2998)
                     /locus_tag="VHT22_06055"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEX3137152.1"
                     /translation="TGFGNAINVMRTVEEFIGAGVAAVHIEDQVAPKRCGHVAGKEVI
                     PLAEMAGKVRAADRVRRELEPEFLLIARCDARGVAGGSVDDLIKRANAYLDAGADMIF
                     PEALTSEAEVERCAREIKGPLHYNRTGVSPRLTLDQLNELGIAIVSNATGALRSSMIA
                     MWDYMNTFAREDVAFVKEFTERHRQHPAGDLHAFVGFADIKKFEQEFLPADDVAKKYA
                     GAVGFQP"
BASE COUNT          433 a          969 c          966 g          630 t
ORIGIN      
        1 tgccggcttt gtctttcggc acgcccttgc ccttgccggg cgccgcttca gccgccttcg
       61 cctcgacgat tttcacgccg ggcttgagct tgtactggcc ttcagtgacc acgcgggtgc
      121 ctgcttccag cccttcttcg atgattgcgg tgccgtcctt gatcgggccg acgcgcaacg
      181 cgcgcaccgc gaccgtgtcg tcggcattca cgacgtacgc gaacagcccg ttggggccgc
      241 gttgcaccgt gcccgccggc accgtcgccg cgcgctggcg cgtgcccatc atcatgtgca
      301 cgttcacgta ctggccgggc cacagcttgt gcgcattgtt cggaaactgc gctttcaact
      361 gcaccgtgcc cgtcgccgtg tcgatctggt tattgacgag gataagcttg ccggtgccca
      421 gcagggtgtt gtcctcgcgc gcgaacgcgc gcacttcgag cgcagtgcgg ccgtgctcct
      481 tcatcgcgcg gttgacgtcc tggaagcggt cctcgggcag ggtgaacagc acggcgatgg
      541 ggttgatctg attgatgctg acgatgccgg tattgtcgga ggcgcgcacg atgttgcccg
      601 gatcgacgag gcgcacgccg gtgcggccgc tgatcggcgc gcgtatggtc gtatagtcga
      661 gctgcacttt cgcgttgtcg atcgtcgcct gatcggcttg caccgttgct ttgagctgat
      721 cgaccgtcgc ctgttgcgtc gtgagcgtct gctgcgcgat cgaatcctgt ttccacaagg
      781 tcgtgtaacg ctccagatcg gtgatgctgt tcttgagctg cgcggagtcg cgcgcctttt
      841 gcgcctgcgc gagctcgagc tgggcctgga acggccgcgg atcgatttgc gcgaggatgg
      901 tgcccgcggt cacgtcctgg ccttcgctga acatcacttt gtcaagctgg ccgtcgacgc
      961 gcgccttgac cgtcaccgcg taaagcggca ttaccgtgcc gatgccggac agataggtcg
     1021 gcacgtcctc ttctttgact gtcgtcgtga ccaccggcac cgccggcggt ccggcttttg
     1081 tggcatccgc ggctttcgtc acgtggaatg aggagcgcca cacgaccgct gccaccagca
     1141 gcacggcaca tgcggctatc aacgtcatcc tcgcccgttt cgtcttcagc atgtaatggc
     1201 cgttcctttc gccgtcagcc ggcacttgcc cggacgtatg ttaattcgaa atgcttccgc
     1261 gtgcttttac gggaggccgg tgccccggat atccccgcct gcagcgcgct gccgcgtgat
     1321 tctccagcag ttattcggcg cgcaacccgg ctgtgcggat cactttgccc catttggcga
     1381 tttcggcttt gatgaactcg ccgaactgct cgggcgtgtt gccgacgggc tccgcgcctt
     1441 ccgcgagcag gttcgctttg acctccggca gattgatgat cgccaccact tcgcggttca
     1501 atttcgtcac gatcgcgcgc gccgtcgctt tcggcgcgag caggccgtac caggtgctcg
     1561 attcgtaccc gcgcacgcct gcttccatca tcgtcggcaa ttcgggcagc gccgccgagc
     1621 ggcgtgcgcc ggtgaccgcc agcgcgcgca gccggccggt cttgacgtgc ggcgtgccgg
     1681 aaatgatgct cgcgaacgtc atcgtgacct gcccgctgat cacgtcgacg atcgccggcg
     1741 agatgccctt atatggcacg tgcgttaagt cgatgccggc gagaattttc agcagctcgc
     1801 ctgacaaatg gccgcccgag ccgttgccgg cggacgcgaa cgtgagctgt cccggtttgg
     1861 ccttcgcgag ctggattagt tcctgcacag tcctggcggc gacggctgga ttcacgagga
     1921 gtacgatttg ctgggatgca acgagcgtga tcggcgcgaa gtcgcgcacc ggatcgtaag
     1981 ggagtttttt tgccaggctc acgttcgtgg ccatcgcagc gatggtcgcc atgatgagcg
     2041 tatagccgtc gggcggcgcc ttggccgtga tttctccgcc gatcacgccg cccgcgccgg
     2101 agcggttatc gacgacgacc tgctgtcccc agcgttggga gagattgctg ccgaccgtgc
     2161 gtgccagggt atcgacgctg ccgcccgcag tggatggcac gatgatgcgg atggggcgcg
     2221 acggatagtc ctgtgcctgg cacacgctcg cgcaggcgag caatgccgct gcgctaagtt
     2281 tgccgcaggt tggcctcacg cctgacgcct cacgccggtc atggctggaa accaaccgcg
     2341 cccgcgtact tctttgcgac gtcgtcggcc ggcaggaatt cctgctcgaa tttcttgatg
     2401 tcggcgaagc cgacgaacgc atgcaggtcc cctgcgggat gctggcgatg gcgctcggtg
     2461 aattccttga cgaacgcgac gtcttcgcgc gcgaatgtgt tcatatagtc ccacatcgca
     2521 atcatcgacg agcgcagcgc gccggtcgca ttgctgacga tcgcaatgcc gagctcgttg
     2581 agttgatcga gcgtcaggcg cggcgagacg ccggtccggt tgtaatgcag cggccctttg
     2641 atctcacgcg cgcagcgttc gacctccgct tcgctcgtga gcgcctcggg aaatatcatg
     2701 tcggcgccgg catcgaggta ggcgttcgcg cgcttgatga gatcgtcgac gctgccgccg
     2761 gcgacgccgc gcgcgtcgca gcgcgcgatc aggagaaact ccggctcgag ttcgcgccgc
     2821 acgcggtcgg ccgcgcgcac cttgccggcc atttccgcga gcggtatcac ttctttgccg
     2881 gcgacgtggc cgcagcgttt gggcgcgacc tggtcttcga tatggaccgc ggccacgcct
     2941 gcaccgatga actcctcgac ggtgcgcatc acgttgatcg cgttgccgaa gccggtgt
//
LOCUS       DATANS010000417         2101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_357789, whole genome shotgun sequence.
ACCESSION   DATANS010000417 DATANS010000000
VERSION     DATANS010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357789"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VHT22_06060"
     CDS             <1..247
                     /locus_tag="VHT22_06060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3137153.1"
                     /translation="AKVRLTVTDDGTGMPVDAVDAAGMGLKIMQYRARILGGEVRFER
                     AEPQGTRIVCECPLEPLAQPARAPRARRRSRLSTAAT"
     gene            300..1028
                     /locus_tag="VHT22_06065"
     CDS             300..1028
                     /locus_tag="VHT22_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3137154.1"
                     /translation="MTVGPSLFKPAHRPSGSGVSKDRKRRVLIVDDHPIVRQGLRRLI
                     EAESDLEVCGEAETAREAKSLIRELEPDAVIVDVSLKQGDGLELVKDARAHYPSLPLL
                     VLSMHDEAIYAERMLSAGANGYIMKQAASDQFLIALRRVLDGGIYVSEAVGNSMIEKF
                     ASGGAYISSNPVDSLTNRELQVLHMIGKGLSTRQTAEALNLSVKTVESHRQRIKRKLS
                     LRTSAQLMQYAVNWYAGREPGQKP"
     gene            1025..>2101
                     /locus_tag="VHT22_06070"
     CDS             1025..>2101
                     /locus_tag="VHT22_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX3137155.1"
                     /translation="MTEALQGLLTGSGIAGLVVGLLLASVPLVWLLLRRGQRSNGPSR
                     RSDAAATNRRNLAIVEGAGEGVLELDNVGQVRYANPAAAKLLGYEIEELPGLDYRVLI
                     NTQTDDDNRTDPVRRVRYTTDIMRGVGALLRRKNGQFRPVEYRIVPVTEQGATVGTVL
                     VFHDVGERVRLDNLLKDMQTTARIGGWEYDVDTHKVHWTEALYSIHDLPFGQTIELES
                     SRNMFHPEDKKKLIEASQHAIDTGASSDLHLRLTSARGRQLWVRMIIKAERRNGKIVR
                     LHGTLQDVTDRVVAERQLRETRDFFELTLNAVPTPINYFNRDFVMTYSNRAFEEWVGL
                     PSTKMIGKGLRELLPTDMFTVVMPY"
BASE COUNT          401 a          663 c          681 g          356 t
ORIGIN      
        1 cgctaaggtg cgcctgaccg taactgacga tggcaccggt atgccggtcg acgcggtgga
       61 tgccgccggc atgggactga agatcatgca ataccgtgcg cgaattctcg gcggcgaagt
      121 ccgcttcgag cgggccgaac cgcaaggcac gcgcatcgtg tgcgaatgtc cgctggaacc
      181 tctggcgcaa ccagcccgcg ccccgcgtgc ccgccgtcgt agccgactga gcacggcagc
      241 gacctgaatc aaccttgttg ccgatgtaac cgacaaggac tattgctagg agtgatgcaa
      301 tgaccgttgg cccctctctg ttcaagcccg ctcatcgacc gtcggggagc ggtgtgtcga
      361 aggaccgcaa gcgccgcgtg ctgatcgtcg acgatcatcc gatcgtccgg cagggcctgc
      421 gccgcctgat cgaggccgaa tccgatctgg aagtgtgcgg cgaggccgag acggcgcgcg
      481 aagcgaaatc tctgatccgc gagctcgagc cggatgccgt catcgtcgac gtgtcgctca
      541 agcagggcga cggcctggag ctggtgaagg atgcacgcgc gcactatccg tcattgccgc
      601 tgctggtgct gtcgatgcat gacgaagcga tctatgccga gcgcatgctg tcggccggcg
      661 cgaacggcta catcatgaag caggctgctt ccgatcagtt cctgatcgcg ctgcgccggg
      721 tgctcgatgg cggcatctac gtgtccgaag cggtcggcaa cagcatgatc gagaagttcg
      781 cgtccggtgg cgcgtacatt tcttccaatc ccgtcgacag cctgaccaac cgtgagctgc
      841 aggtgctgca catgatcggc aagggtctgt cgacgcgaca gacggccgaa gcgctgaacc
      901 tcagcgtcaa gaccgtcgag tcgcatcgcc agcgcatcaa gcgcaagctg tcgctgcgca
      961 cttcggcgca gctgatgcaa tacgctgtga attggtacgc gggccgggag cccggacaga
     1021 aaccttgacc gaagcgctgc aaggactgct caccggttcc gggatcgcgg gtctcgtcgt
     1081 cggactgttg ctggcaagcg tgccgcttgt ctggctgctg ctgcgtcgtg gccagcgcag
     1141 caacgggccg tcgcggcgga gcgatgcagc cgcgaccaat cgacgcaacc tggcgatcgt
     1201 cgaaggcgcg ggcgagggtg tgctcgagct cgataacgtc ggccaggtgc gttacgcgaa
     1261 tcctgccgcg gcgaagctgc tcggttacga gatcgaggag ctgcccggtc tcgactaccg
     1321 cgtcctgatc aacacccaga ccgacgacga caaccggacc gatccggtgc gccgcgtgcg
     1381 ctacacgacc gacatcatgc gcggcgtcgg cgcgctgctg cgtcgtaaga acggccagtt
     1441 ccgtcccgtc gaatatcgca tcgtgccggt caccgagcag ggcgcgacag tcggcaccgt
     1501 actcgtgttc cacgatgtcg gcgagcgcgt gcgtctcgac aacctgctga aggacatgca
     1561 gacgactgcg cgcatcggcg gttgggaata cgacgtcgac acgcacaagg tccactggac
     1621 cgaagcgctg tattcgatcc acgacctgcc gttcggccag acgatcgagc tcgaatccag
     1681 ccgcaacatg ttccatccgg aggacaagaa gaagctgatc gaagcttcgc agcacgcgat
     1741 cgacaccggt gccagcagcg atctgcatct gcggctcacc agcgcgcgcg gccggcagct
     1801 gtgggtgcgc atgatcatca aagcggaacg acgcaacggc aagatcgtgc gactgcacgg
     1861 cacgttgcag gacgtcaccg atcgcgtggt cgccgagcgc cagctgcgcg agacgcgcga
     1921 cttcttcgag ctgacgctga acgcggtgcc gacgccgatc aactatttca atcgcgactt
     1981 cgtcatgacg tactcgaacc gcgcgttcga agagtgggtc ggtctgccct cgacgaagat
     2041 gatcggcaaa ggtctgcgcg agctgctgcc cactgacatg ttcacggtcg tcatgccgta
     2101 c
//
LOCUS       DATANS010000418         1795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_525775, whole genome shotgun sequence.
ACCESSION   DATANS010000418 DATANS010000000
VERSION     DATANS010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525775"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..609
                     /locus_tag="VHT22_06075"
     CDS             76..609
                     /locus_tag="VHT22_06075"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX3137156.1"
                     /translation="MHDTPAPAAPPSAAPRIALIAAVARNGVIGLHGRLPWRLPDDLK
                     RFRALTSGHAIVMGRKTWESIGKPLPERQNIVVSRHLDFAAPGAVVAHSLPEAIGLAA
                     MPSPLFVIGGEAIYRDALPLADHLFLTEIHRQFDGDAKFPDFDRTQWRETARESRRLD
                     GDSGFVYDFASYSRLRP"
     gene            647..1216
                     /locus_tag="VHT22_06080"
     CDS             647..1216
                     /locus_tag="VHT22_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HEX3137157.1"
                     /translation="MSEDHFHVHGPHEHEAEHQAQHDPFAGRIAVMTAIFATIGALFG
                     YMAGHTQNEALLFKNEAAIRRTEASDQWNFYQAKSAKQNLAELGATLTTGEQQARYQK
                     EVDRYKQEKEEIMPKAKKLEDQSSEAEQKSEASMHVHHRWAQAMTLIQIAIALAAITI
                     LTRNRRLQYVSYAGAAISIALGALAVAHI"
     gene            complement(1233..>1795)
                     /locus_tag="VHT22_06085"
     CDS             complement(1233..>1795)
                     /locus_tag="VHT22_06085"
                     /inference="COORDINATES: protein motif:HMM:NF044969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEX3137158.1"
                     /translation="DMAETIAHFAPRIEALASRLPQLLDAAERSRVDAEIARYEAQAV
                     PHELAARVATFDTLYATLDIIEVAEATQRPVELVADVYFDLATRLGLAWLRDRIAALP
                     GDQHWQMLARGAMQDDLSGLQRTIAADVMGGADAATAALLIGAWQDRNRRSIERAVQL
                     LGELRMAAQPDAAMLSVALRELRNLG"
BASE COUNT          336 a          610 c          570 g          279 t
ORIGIN      
        1 cgcagcatca ggggcaaagc cgccgcgaac tatcgagcga atttctgact cagtacacca
       61 gccagcgagc cacgcgtgca cgatacacca gcccctgccg ccccgccaag cgcggcgccc
      121 cggattgcgc tgattgccgc cgttgcgcgt aatggcgtga tcggcctgca cggccgattg
      181 ccttggcggt tgcccgacga tctcaagcgc tttcgcgcgt tgaccagcgg tcacgcgatc
      241 gtcatgggtc gcaagacctg ggagtcgatc ggcaagccgc tgcccgaacg gcaaaacatc
      301 gtcgtgagtc gacacctcga cttcgccgcg cccggcgccg tcgtcgcgca ttcgctgccg
      361 gaagcaatcg gcctcgccgc tatgccctcg ccgctgttcg tcatcggcgg tgaagccatc
      421 tatcgcgatg cgctgccgct ggcggaccac ctgttcctga ccgagatcca tcgccaattc
      481 gacggcgatg cgaaattccc ggacttcgac cgcacgcaat ggcgtgaaac agccagggaa
      541 tcgcgccggc tggacgggga ttccggcttc gtctacgatt tcgcgtccta ttcgcggctg
      601 cgcccatagg gcgcgcgctt caactgcctt cgcttggaga actcgcatgt ccgaagatca
      661 ctttcacgta cacggtccgc acgagcatga agccgagcat caagctcagc acgatccgtt
      721 tgccggccgc atcgcggtga tgacggccat tttcgcgacc ataggcgcgt tgttcggcta
      781 tatggccggg cacacacaga acgaggcgct gctgttcaag aacgaagcgg cgatccgcag
      841 gacggaagcg tcggatcagt ggaatttcta ccaggccaaa agcgcgaagc agaacctcgc
      901 cgagctcggc gccacgctga ccaccggcga gcagcaggcg cgttatcaga aggaagtcga
      961 tcggtacaag caggaaaaag aagagatcat gcccaaggcc aagaagctgg aggaccagtc
     1021 gagcgaggcc gagcaaaaga gcgaggcgtc gatgcacgtc caccatcgct gggcccaggc
     1081 gatgacgctt atacagatcg ccatcgcgct cgccgcgatc acgatcctga cccggaaccg
     1141 gaggctgcaa tacgtttcgt acgcgggcgc cgcaatttcg atcgcgctgg gcgcgctcgc
     1201 ggtcgcgcac atctgaagcg gccggccgcc gcctagccga ggttgcgcaa ctcccgcagt
     1261 gcaaccgaga gcatcgcggc gtcgggctgc gcggccatgc gcagctcgcc cagcagctgc
     1321 accgcacgct cgatgctgcg ccggttgcga tcctgccagg caccgatcag cagagcggcc
     1381 gtcgcggcat cggcgccgcc catcacgtcg gcggcgatcg tccgctgcag gccggacaga
     1441 tcgtcctgca tcgcgcccct cgccagcatc tgccagtgct ggtctccagg aagcgcggca
     1501 atcctgtcgc gcagccacgc gaggccgagt cgcgtcgcaa ggtcgaaata gacgtcggcg
     1561 acgagctcga ccggccgctg cgtcgcctcc gcgacttcga tgatgtcgag cgtcgcatac
     1621 agggtgtcga acgtggcgac ccgcgccgcg agctcatggg gcaccgcctg cgcctcgtag
     1681 cgggcgatct cggcatcgac ccgcgagcgc tcggcggcat cgagcaactg gggcaggcgc
     1741 gaggccagcg cctcgatccg cggcgcaaaa tgagcgatcg tctcggccat gtcgt
//
LOCUS       DATANS010000419         1818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_695145, whole genome shotgun sequence.
ACCESSION   DATANS010000419 DATANS010000000
VERSION     DATANS010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695145"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1076
                     /locus_tag="VHT22_06090"
     CDS             156..1076
                     /locus_tag="VHT22_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX3137159.1"
                     /translation="MHLIRKFEETAEASYMRGLIHGTMHLSIGQEASAVGATLELARD
                     DYILSTHRGHGHCIAKGADPARMFAEFFGKENGYCRGRGGSMHIADIEGGNLGANGIV
                     AGGLPIAVGVGMSIKAQKQHRVCMVFFGDGAANEGAFHESLNMASIWRLPIVFVCENN
                     KYGMSMDIARAMAVANVADRAPAYAMPGVTVDGNDLPVVVAAARPAIERARQGGGPTL
                     LECKTYRLRGHSKSDRNLYRSKEEIEAWREKDAIRRLESELIAHGRFDEGELLAIEQA
                     AQKTIDDALEFAKASPNPDPAQLTRDVYAD"
     gene            1066..>1818
                     /locus_tag="VHT22_06095"
     CDS             1066..>1818
                     /locus_tag="VHT22_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX3137160.1"
                     /translation="MPTELVVPEAGTRELSYAEAIREGLAQAMQADERVFLFGEDVGV
                     YGGAFGVSGDLFHKFGPERVIDTPISELGIAGAAVGAAITGMKPVLELQFSDFVTLAM
                     EQIVNQAAKIHFMFGGKATVPMVVRLPGGSGTGAAAQHSQSLEAWFAHVPGLKVVQPS
                     TPHDAKGLLLAAIDDPNPVLIFEHKLLYKTKGPVPEEPYRVPIGKAAVRRAGRDLTIV
                     GSSIMAIRAVAAAARLAGENIDAEVIDLRSLRP"
BASE COUNT          325 a          599 c          622 g          272 t
ORIGIN      
        1 atgaccggcg gcagctgtcg cgcggcgctg tcgttcgccg cggcggactg atcgtggcta
       61 tcgctcccgg cagccgcacc atcgccgcgc aggcgaccga gcgtccggag ctcgccgcgc
      121 tctccgatga cgggctgcgc gtggcattgt cgcggatgca cctcatccgc aagttcgaag
      181 agaccgccga agcgagctac atgcgcgggc tcatccacgg cacgatgcac ttgtcgatcg
      241 ggcaggaagc gagcgcggtc ggcgcgacgc tcgagctcgc gcgcgacgac tacattctct
      301 cgacgcatcg cggccacggc cattgcatcg ccaaaggcgc cgatccggcg cgcatgttcg
      361 ccgagttctt cggcaaggaa aacggctatt gccgcgggcg cggcggctcg atgcacatcg
      421 ccgacatcga aggcggcaat ctgggcgcca acggcatcgt cgccggcgga ttgccgatcg
      481 ccgtcggcgt cggcatgagc atcaaggcgc agaagcagca tcgcgtctgc atggtgtttt
      541 tcggcgacgg cgccgccaac gagggtgcct tccacgagtc gctcaacatg gcgtcgatct
      601 ggcggctgcc gatcgtgttc gtctgcgaaa acaacaagta cggcatgtcg atggacatcg
      661 cccgcgcaat ggccgtggcc aacgtcgccg accgcgcgcc ggcgtacgcg atgccagggg
      721 tcaccgtcga cggcaacgac ctgcccgtag tcgtcgcggc ggcaaggccg gcgatcgaac
      781 gcgcccggca gggcggcggt ccgaccttgc tcgaatgcaa gacctaccgg ctgcgcgggc
      841 attccaagag cgatcgcaac ctctatcgca gcaaggagga aatcgaagcc tggcgcgaaa
      901 aggatgcgat tcgccgtctc gagagcgagc tgatcgcgca cggccgcttc gacgaaggcg
      961 aattgctcgc gatcgaacag gcggcgcaga agacgatcga cgatgcgctg gaattcgcca
     1021 aggcgagccc caaccccgac ccggcccagc tgacccgcga cgtctatgcc gactgagctt
     1081 gtcgtccccg aagccggaac gcgagaattg tcgtacgccg aggcgatacg cgaaggcttg
     1141 gcgcaggcga tgcaggcgga cgagcgcgtc ttccttttcg gcgaggacgt gggcgtgtat
     1201 ggcggcgcgt tcggtgtgtc cggcgacctg ttccacaagt tcggccccga acgggtgatc
     1261 gatacgccga tcagcgagct ggggatcgcc ggcgcggcgg tcggggccgc gatcacaggc
     1321 atgaagccgg tgctcgagct gcaattctcg gacttcgtga ccttggcgat ggagcagatc
     1381 gtcaaccagg cggcaaagat ccacttcatg ttcggcggca aggccacggt gcccatggtc
     1441 gtgcggctgc cgggcggatc gggcaccgga gcggcagcgc agcacagcca gagcctcgaa
     1501 gcctggttcg cccatgtccc gggcctgaaa gtcgtgcagc cgtcgacgcc gcacgatgcc
     1561 aaaggcctgt tgctggcggc gatcgacgat ccgaacccgg tgctgatctt cgagcacaag
     1621 ctgctttaca agacgaaggg cccggtgccg gaagaaccct accgcgtacc gatcggcaag
     1681 gcggcggtcc gccgcgccgg gcgcgacttg acgatcgtcg gcagctcgat catggcgata
     1741 cgcgccgtcg cagccgccgc gcgcctcgcc ggcgagaaca tcgacgccga ggtcatcgac
     1801 ctgaggtcgc tccgcccg
//
LOCUS       DATANS010000420         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_191271, whole genome shotgun sequence.
ACCESSION   DATANS010000420 DATANS010000000
VERSION     DATANS010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191271"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VHT22_06100"
     CDS             <1..251
                     /locus_tag="VHT22_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX3137161.1"
                     /translation="VQSQLDAAASAHFYAATPAARTMGAAEFTFGLNSTAKDPLCPDS
                     DFKIKDGHVDVPRGHGLGVTIDEALLKKNTLMQDSIGK"
     gene            341..607
                     /locus_tag="VHT22_06105"
     CDS             341..607
                     /locus_tag="VHT22_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137162.1"
                     /translation="MLCVKTTAVAVAALLFSPGAWAQSLAANYPNKVVRFICPFPPGG
                     ANDLLARLFAQKMTDAYGQQVIVDNRGGAGGQTRAVNRVPACGM"
     gene            730..1566
                     /locus_tag="VHT22_06110"
     CDS             730..1566
                     /locus_tag="VHT22_06110"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX3137163.1"
                     /translation="MSRAIGEKVGAAWGQQVVVDNRSGAGGNIGTETVARSAPDGYTV
                     MIGHVGTHAINVSLYPQVNFDPVRDFTPITLVATYPLALVIYPGVAAKSVPELIALAK
                     AKPGALNLASAGSGGPTHLAGEVLKSLAHVNIVHIPYKGNAAALTALVGGEAHMMFSN
                     LLTSMPHVRSGRLRVLAVSTARRSPQMPDLPTIAESGVPGYDVTPWYGILGPAHLPPA
                     IVAKWNREVARVVALPEMNERFVVQGVDLGASTPEAFANLIKAEVPRWRKIVRDSGAR
                     AD"
BASE COUNT          304 a          532 c          505 g          273 t
ORIGIN      
        1 ccgtgcagtc gcagctcgac gccgccgcca gcgcgcactt ctacgccgct acgcctgcgg
       61 cgcgcacgat gggcgcggcc gagttcacgt tcggcctgaa ctcgacggcg aaagacccgt
      121 tatgccccga cagcgatttt aaaatcaagg acgggcacgt ggacgtgccg cgcggtcacg
      181 gcctgggcgt caccatcgat gaagcgttgc tgaagaaaaa tacgctgatg caggacagta
      241 tcggcaaata accgtttacg taacgcggcg tcgaccaggc agccggctcc agaccggctc
      301 tgaccgaact gtgcgtagcc ccacgtgagg aggatcgacc atgttgtgcg tcaaaaccac
      361 ggccgttgcc gttgcggcac tgctgttctc gccaggcgcg tgggcgcagt ccctagccgc
      421 gaactatccc aacaaggtcg tccgcttcat ctgtccgttt ccgccgggcg gcgccaatga
      481 tctgctggcg cgcctgttcg cgcagaaaat gacagatgcg tacggacagc aggtgattgt
      541 cgacaatcgc gggggcgccg gcggccagac gcgagcagtg aacagggttc ccgcttgcgg
      601 gatgtgaacg tccgcgagtc gggatcggcc gccgggtttt atcgcgttgc cgatagcaaa
      661 tccacaccat atccgagcaa acccattcac tacatcgtcc ctttcgcacc cggcggcccg
      721 accgacatca tgtcgcgcgc gatcggcgaa aaagtcggcg cggcgtgggg acagcaggtc
      781 gtggtcgata atcgcagcgg cgccggcggc aacatcggca ccgaaactgt tgccagatca
      841 gcgccggatg gctacaccgt gatgataggg cacgtcggca ctcacgcgat caacgtgagc
      901 ctctatccgc aggtaaattt cgaccctgtc cgggatttca cgccgataac gctcgtcgcc
      961 acctatccgc tggcgctggt catctatccc ggcgttgccg cgaaatcggt gccggaactg
     1021 atcgcactcg ccaaggcgaa gccgggcgcc ctgaatttag cctcagccgg cagcggcggg
     1081 ccgacgcatc tcgccggcga agtcctgaag agtctcgcgc acgtaaacat cgtgcacatt
     1141 ccttacaagg gcaatgcggc agcgctaacc gccctcgtgg gcggcgaagc gcacatgatg
     1201 ttcagcaacc tgctgacgtc catgccgcac gtgcgcagcg gccggctgcg cgtgttggcg
     1261 gtatctaccg cccggcgctc gccgcaaatg cccgacttgc cgactatcgc cgagtccggg
     1321 gtgcccggct acgacgtcac accatggtac ggcatactcg gccccgcgca tctgccccca
     1381 gcgatcgtcg ccaagtggaa ccgcgaagtc gcgcgcgtgg tcgcgctgcc ggaaatgaac
     1441 gagcgcttcg tcgtgcaggg cgtggatctc ggcgccagca cgcccgaagc gttcgcgaac
     1501 ctgatcaagg ccgaagtccc gcgctggcgc aagatcgtgc gcgactccgg tgcgcgcgcc
     1561 gactgatttg cgttttccgc caggccgtga attgcagctg cgattaacgg cgca
//
LOCUS       DATANS010000421         2609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_274753, whole genome shotgun sequence.
ACCESSION   DATANS010000421 DATANS010000000
VERSION     DATANS010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274753"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VHT22_06115"
     CDS             <1..281
                     /locus_tag="VHT22_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3137164.1"
                     /translation="GMSATQILIRIELPLALPVIVAGLRIATVTVISVAVVAAYVNAG
                     GLGTLIFSGISNDHAPKIWTGALTACALAIVADLSLARLESWLRRRAR"
     gene            299..934
                     /locus_tag="VHT22_06120"
     CDS             299..934
                     /locus_tag="VHT22_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3137165.1"
                     /translation="MLVEAWQFIVAHPDRFFGALLTHVTLSAAALALATAIALPTGVW
                     LAGTRRAASIAIATANVGRTLPSLAVLALVMPLLGTGFLPSLFALTLLALPPILLNSY
                     TGIRQVEPEIVDAARGMGMTRGEIVRKVELPIATPVIFAGIRTAAVQVVSGAVLAAYI
                     GGGGLGDFITAGIAMMALPQLLVGAIPATLLALGADQLFGRLQRALTPKGL"
     gene            957..1931
                     /locus_tag="VHT22_06125"
     CDS             957..1931
                     /locus_tag="VHT22_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3137166.1"
                     /translation="MIDIIGLVKQFPGTARPAVDHLTLTIADGEVCVLIGPSGCGKTT
                     TMRVINRMIEPDAGTVKVRGRDIFDIDPVSLRRSIGYVIQQVGLFPHWTIAENVATVP
                     RLLGWKPAKIRTRTDELLTLVGMAPEIYRDRVPRELSGGQKQRVGVARALAADPPVML
                     MDEPFGALDPITRARLQGEFLNILRGLKKTIVFVTHDIDEAIRMGDRIAILKDGALVQ
                     YDTPERILAAPADSFVEAFVGADRALKRLSLIAVTSACEPWPGTPPPNTIAAEASLRD
                     ALARMLEADVDRLAVVDADERFVGALTLQAIRAHSADAAPVARIQRFP"
     gene            2108..2575
                     /locus_tag="VHT22_06130"
     CDS             2108..2575
                     /locus_tag="VHT22_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX3137167.1"
                     /translation="MIRYLEDFEPGQKFGSGRLSVEATRIKSFAAEFDPQPFHLDDVT
                     ARDTLFKGLVASGWHTAAMTMRLLVDSELAPAGGIIGAGFEEMRWPRPVRPGDELHVE
                     SEILDVRPSKSRPDQGLIKVKTTTLNQNGEPVQVFVGNLLVLRRSASSGEEHG"
BASE COUNT          429 a          852 c          852 g          476 t
ORIGIN      
        1 tggggatgag cgcgacgcag attctaatcc ggatcgagct gccgctggcc ttgccggtga
       61 tcgtcgcggg actgaggatc gccacggtca ccgtgatcag tgtcgccgtc gtcgccgcgt
      121 acgtgaacgc cggcggcctg ggcacgctca tcttcagcgg catcagcaac gatcacgcgc
      181 cgaagatctg gaccggcgcc ctcaccgcgt gcgcgctggc gatcgtcgcc gatctgagtc
      241 tcgcccggct cgagagctgg ctgcgacggc gcgcccgcta ggaagcgcta aagtcatgct
      301 gctggtcgaa gcctggcagt tcattgtcgc gcatccggat cgtttcttcg gcgcgctgct
      361 gacccacgtc acgctctcgg ccgccgcgct cgcgcttgcg accgccatcg cgctgccgac
      421 cggcgtgtgg ctcgccggta cgcgtcgagc cgcatcgatc gcgattgcga cggcgaacgt
      481 cggccgcacg ctgccgtcgc tggcggtgct cgcactcgtg atgcctctgc tcgggacggg
      541 tttcctgccg tcgctctttg ctttgacgct cctcgcgctg ccgccgatcc tccttaacag
      601 ctataccggg atcaggcagg tcgagcccga gatcgtcgat gccgcgcgcg gcatgggcat
      661 gactcgcggt gagatcgtcc gcaaggtcga gctgccgatc gcaacgccgg tgatcttcgc
      721 cggcatacga acggctgcgg tgcaggtcgt gtcgggcgcg gtcctcgcgg cgtacatcgg
      781 cggcggcggt ctgggcgact tcatcacagc ggggatcgcg atgatggcct tgccgcaact
      841 cctcgtcggc gcgattcccg cgacgctgct cgcgctcggc gccgatcagc tgttcggccg
      901 cttgcagagg gcgctcacgc ccaaaggcct ctgatggccg cgcccgtcga ccgcagatga
      961 ttgacatcat cggcctcgtc aagcagtttc ccggcaccgc gcgtccggcg gtcgatcatc
     1021 tcacgctgac gatcgccgac ggcgaggtat gcgtgctcat cggcccctcc ggttgcggca
     1081 agacaaccac catgcgcgtg atcaaccgga tgatcgaacc cgacgccggg acggtgaaag
     1141 tgcgcggtcg cgacattttc gacatcgatc cggtgagcct gcgccgcagc atcggctatg
     1201 tcattcagca ggttggtttg tttccgcatt ggacgatcgc cgaaaacgtg gccacggtgc
     1261 cgcggttact gggctggaaa ccggcgaaga tacgaacgcg caccgatgag ctgctgacgc
     1321 tggtcggcat ggcgcccgag atctaccgcg atcgcgtccc gcgcgagctg tcgggtgggc
     1381 agaagcaacg ggttggtgtt gcccgcgcgc tggcggccga cccgccggtg atgcttatgg
     1441 acgagccgtt cggcgcgctc gatcctatta cgcgcgcgcg gttgcagggc gagtttctca
     1501 acatcctccg cgggctgaag aaaacgatcg tcttcgtcac ccacgacatc gacgaagcga
     1561 tcaggatggg tgatcgtatc gctattctga aggacggagc gctcgtgcaa tacgacacgc
     1621 cggagcgaat cctggccgcg ccggccgact cgttcgtcga agcattcgtc ggcgccgatc
     1681 gggcactcaa gcgcctgtcg ctgatcgcag tgacgagcgc ctgcgagccg tggccgggca
     1741 ctccgccgcc gaataccatc gccgcggagg ccagcttgcg cgacgcactc gcgcgcatgc
     1801 tagaggcgga cgtcgacagg cttgccgtgg ttgacgcaga tgagcgtttt gtaggtgcgc
     1861 tcacgctcca agcgatccgg gcgcactctg ccgacgctgc gccggtcgcc cgcatccaac
     1921 gctttccgtg acgccgagtt tttatgttgt ggcgattgac caaggcgcgg aaagaattcg
     1981 agcaaaggtt cgacgccagg cgcgccgtcc atctgcgcgg ccttttgagc aaggtcgttg
     2041 cgtgcgatcc cgaaaagccg cacgtcgctt ccgcagcccg acgccagcca acgcaagcga
     2101 ggctcgcatg atccgatatc ttgaggactt tgaaccgggg cagaagttcg gctcgggccg
     2161 tctctccgtc gaagctacgc gcatcaaatc ctttgccgct gaattcgatc cgcagccctt
     2221 tcatctcgac gacgtcaccg cacgcgacac tttgttcaag ggactggttg cgagcgggtg
     2281 gcacacggcg gcgatgacga tgcgcctctt ggtcgacagc gagctcgcgc ccgccggcgg
     2341 catcatcggt gccgggttcg aggaaatgcg ctggccgcgc cccgtgcggc cgggcgacga
     2401 gctccacgtc gaaagcgaga tactcgacgt gcgtccttcg aagtctcgcc ccgaccaggg
     2461 tttgatcaag gtaaaaacga cgacgttgaa tcagaacggt gagcccgtgc aggtgttcgt
     2521 cggcaatctg ctcgtgctcc gccgttccgc ctcctcggga gaagaacatg ggtaagctga
     2581 aggcaaaacc gcgctcgtac tggcaagca
//
LOCUS       DATANS010000422         2954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_105564, whole genome shotgun sequence.
ACCESSION   DATANS010000422 DATANS010000000
VERSION     DATANS010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105564"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VHT22_06135"
     CDS             complement(<1..324)
                     /locus_tag="VHT22_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3137168.1"
                     /translation="MPQQKEILIVDDEVGIRELLSEILQDEGYQVSVAENAGEARNYR
                     LRTQPALVLLDIWMPDTDGVTLLKEWSTTGLLSMPVVMMSGHATIETAVEATKIGAFD
                     FLEKPI"
     gene            complement(324..2480)
                     /locus_tag="VHT22_06140"
     CDS             complement(324..2480)
                     /locus_tag="VHT22_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3137169.1"
                     /translation="MTRILAPRALRYVLLGCAVLGTVFLFLLATASANTELFASNYNL
                     LLVLNGTMVALLMGLVGYQLWRLRKNLKAGVFGSRLAIRLVLLFALVAVLPGALLYGV
                     SVQFLGKSIESWFDVRVDKALEGGLNLGRGALDYLLKETTNKASQLALTLQEGDAASM
                     PQRLNRASEQAGIYEAALFTSGGGVLGVAGISGSTLTPEPPPAQALRRARLQQPYSAI
                     DQTTDQGMLLRVVVPVNTSDPGEPLRLLQVVEPVPRQLQLDAEKVQAGYRDYQEISFS
                     RAALKRLYALTLTLTLLLALFSALGLAVVLSEQVSGPLGLLAEGTRAVAQGDFSRRHP
                     VQSRDELGVLTESFNTMTAQLAEAQQKTAESRNAIETTNAYLESILSNLSAGVLAFDS
                     AFRLRTANPSAAVILQQPLADLIDVRLSDWAGGLPPLAGFAELVADGFRGGRDGQWQR
                     QAEVRVSNHTRVLLMRGSRLPGEPAAGFVVVFDDVTELAEAQRDAAWAEVARRLAHEI
                     KNPLTPIQLSAERLAVKLGGKLAAGDEEALTRATRTIVAQVGAMKHMVDDFAIYARQS
                     GPGRMQPVDINALLLDVLGLYENLRPHVSLKLVEGDPVIHGEPTRLRQVIHNLLQNAI
                     DAQAESPDPAYDIGLEVRGDEVALSIGDRGGGFPDDVLRRAFEPYVTTKAKGTGLGLA
                     IVKKIAEEHHGRVTIANRSPHGAVVTLYLPFKGTNT"
     gene            complement(2477..>2954)
                     /locus_tag="VHT22_06145"
     CDS             complement(2477..>2954)
                     /locus_tag="VHT22_06145"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEX3137170.1"
                     /translation="IGVKSAELLPEDEDYVLNAQFDLAFNPTLEEALQKGVSLYFVLE
                     FELVRPRWYWLDQKLVQMSVQYRLTYNPLTRQYRVTSGLLGQQFDSLDEVERLLARVV
                     SRPVVRKDALSPGERYEAAVRLRLDVAQLPKPFQINALASRDWSLQSEWFRWSFTP"
BASE COUNT          525 a          981 c          938 g          510 t
ORIGIN      
        1 gatcggcttc tcgaggaaat cgaaggcgcc gatcttggtc gcctcgacgg cggtctcgat
       61 cgtcgcgtgg ccgctcatca tcaccaccgg catgctcaga aggccggtgg tcgaccactc
      121 cttcagcagc gtcacgccgt cggtgtccgg catccagatg tcgagcagca ccagtgcggg
      181 ttgcgtgcgc agccggtagt ttcgcgcctc tccggcattt tcagccacac tgacttgata
      241 tccctcgtcc tgcagaatct cggacaacag ttcgcggatg ccgacttcgt cgtcgacgat
      301 cagaatttct ttctgctgcg gcatcacgtg ttggttcctt tgaacggcaa gtacaaggtc
      361 acaaccgctc cgtgcggcga ccgattcgcg atggttaccc ggccatggtg ctcctcggcg
      421 atcttcttga cgatcgcgag cccgaggccg gtgcctttcg ccttggtcgt cacatacggc
      481 tcgaacgcgc gacgcaggac atcgtcggga aatccgccgc cgcggtcgcc gatcgacaac
      541 gcgacctcgt cgccgcgcac ctcgaggccg atatcgtacg cggggtcggg cgattccgcc
      601 tgtgcatcga tggcattctg gagcagattg tggatcacct ggcgcagccg cgtcggctcg
      661 ccgtgtatga cgggatcgcc ttccacgagc ttgagcgata catggggccg cagattttcg
      721 tacagaccga gtacgtcgag caacagcgca ttgatgtcga ccggttgcat ccttcccggt
      781 cccgattgcc gggcgtagat cgcaaaatcg tcgaccatgt gcttcattgc gcccacctgc
      841 gcgacgatcg tccgcgtcgc ccgggtcagg gcctcctcgt cgcccgcggc cagcttgccg
      901 ccgagcttga ccgcgaggcg ctccgccgag agctggatcg gcgtcagcgg attcttgatc
      961 tcgtgcgcga ggcggcgtgc aacctccgcc caggcagcat cgcgctgcgc ctcggccagt
     1021 tcggttacat cgtcgaagac gacgacaaac ccggcggccg gctcaccggg caacctcgac
     1081 ccccgcatca gcagcacgcg cgtgtgattc gatacgcgga cctcggcctg acgctgccac
     1141 tggccatcgc gcccgccgcg aaagccgtcg gccaccagct cggcaaagcc ggcgagcggc
     1201 ggcaggccgc ccgcccagtc ggaaagccgc acgtcgatca gatcggccag cggctgctgc
     1261 aggatcaccg cagcgctcgg gttcgcggtg cgcaggcgaa acgcgctgtc gaacgccagc
     1321 accccggccg atagattgga aagaatgctt tcgagataag cgttcgtcgt ttctatcgcg
     1381 tttcggcttt ccgcggtttt ttgctgcgct tcggcgagct gcgcggtcat cgtattgaac
     1441 gactcggtca ggacgcccag ctcgtcccgg ctttgcaccg gatgccgacg gctgaagtcg
     1501 ccttgtgcga cggcgcgcgt gccctcggcc agaagcccca acggtccgct gacctgctcg
     1561 gagagcacga cggccaagcc aagcgcggag aaaagcgcga gcagcaaggt caaggtgagt
     1621 gtgagtgcgt aaaggcgctt cagcgccgcg cgtgaaaagg agatctcctg gtagtcgcgg
     1681 tagccggcct gcactttttc ggcatcgagc tgcaattgtc ggggcaccgg ctcgacgacc
     1741 tgcagcaacc gcagcggctc gccgggatca gacgtattga ccggaacgac gacgcgcagc
     1801 agcatgccct ggtcggtcgt ctgatcgatc gccgagtagg gttgctgcaa cctggcgcgc
     1861 cgcagcgcct gcgccggcgg cggctccggc gtcagcgtcg agcccgaaat tcccgcgacg
     1921 ccaagcacgc cgcccccgga cgtgaacagc gcggcttcgt atattcccgc ctgctcactg
     1981 gcgcgattga gccgctgcgg catgctggct gcgtcgcctt cctgcaaggt cagcgcgagc
     2041 tgcgaagcct tgttggtcgt ttccttcagc agatagtcga gcgcgccgcg gccgagattc
     2101 aatcctcctt cgagcgcctt gtcgacgcgc acgtcgaacc atgattcgat gctcttgccg
     2161 aggaactgca ccgacacgcc gtaaaggagc gcgccgggaa gcaccgctac cagcgcgaac
     2221 aggagcacca gcctgatcgc gagccgcgac ccgaaaaccc ccgccttgag gttcttgcgc
     2281 aggcgccaaa gctgatatcc gacgagcccc atcagcaggg caaccatcgt tccgttgagc
     2341 accagcagca gattgtagtt gctggcgaag agctcggtat tcgcgctcgc cgtggcgagc
     2401 aggaacagga acaccgtgcc gaggacggcg cacccgagca gcacgtagcg cagcgcgcgc
     2461 ggtgcgagaa tcctggtcac ggcgtgaagc tccagcgaaa ccattccgac tgcagcgacc
     2521 agtcgcgcga ggccagcgca ttgatctgga acggcttcgg cagctgggca acgtccaggc
     2581 gaagacgcac ggcggcttca tagcgctccc cgggcgacag cgcgtccttg cggacgaccg
     2641 ggcgcgagac gacgcgggca agcaggcgct ccacctcgtc gagcgaatcg aactgctggc
     2701 cgagcagtcc gctggtcacg cggtactgtc gggtcagcgg gttgtaggtc aagcggtact
     2761 gcaccgacat ctgcaccagc ttctgatcga gccagtacca gcggggccgc acgagctcga
     2821 actcgaggac gaaatagagc gagacgccct tttgcagcgc ctcctcgagc gtcggattga
     2881 aggcgaggtc gaactgcgca ttcaggacat agtcttcgtc ctccggcagg agctcggccg
     2941 atttgacccc gatc
//
LOCUS       DATANS010000423         1710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_652678, whole genome shotgun sequence.
ACCESSION   DATANS010000423 DATANS010000000
VERSION     DATANS010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652678"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VHT22_06150"
     CDS             <1..502
                     /locus_tag="VHT22_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1134 domain-containing protein"
                     /protein_id="HEX3137171.1"
                     /translation="QRQAGPDTFEPGELVSAGHKFFGNVSRGLASVIERAVSQWGLPN
                     GYVLGEEGSGAVVAGLRYGEGTLYTKNAGDLRVYWQGPSVGFDWGGDGARTMTLVYNL
                     PATNAIYQRFVGIDGSAYIIGGFGMTALTANNIVLVPIRSGIGLRLGANVGYLKYTPR
                     ATWNPF"
     gene            632..1159
                     /locus_tag="VHT22_06155"
                     /pseudo
     CDS             632..1159
                     /locus_tag="VHT22_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600524.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1136..1513)
                     /locus_tag="VHT22_06160"
                     /pseudo
     CDS             complement(1136..1513)
                     /locus_tag="VHT22_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600524.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          322 a          552 c          548 g          288 t
ORIGIN      
        1 gcaacgccag gcggggccgg acacgttcga acccggcgaa ctggtttcgg ccgggcacaa
       61 gttcttcggc aacgtctcgc gcgggctggc gtccgtcatc gagcgcgcgg tcagccagtg
      121 gggccttccc aatggctacg tactcggcga ggaaggctca ggcgctgttg tggcaggcct
      181 gcggtatggc gaaggcacgc tctacaccaa gaacgccggc gacctgcggg tgtactggca
      241 gggaccgtcg gtcggcttcg actggggcgg cgacggcgcg cgcaccatga cgctggtcta
      301 caacctgccc gccaccaacg cgatctatca gcgcttcgtc ggcatcgacg gctccgccta
      361 tatcatcggc ggcttcggca tgaccgcgct gaccgccaac aacatcgtac tggtgccgat
      421 ccgctccggc atcggcctgc ggctcggcgc caatgtcggc tatctcaaat ataccccgcg
      481 cgcgacctgg aacccgttct gagctcatcc tcaggcatag acgcgcatct ccccaggcga
      541 attcaggtta acgcggcatt ggactggcgc agggccggcc gggcgtggca atgtgattga
      601 cgaatcctca tctcgtgcgg agtaaccatc gatgatcgaa ccgatcatgt acctggcgat
      661 cggttttctg gtctccatgc tgttcggtct gatgatcgtg ccgctggtgc ataaccgcgc
      721 ggtgcggctg acgacgcggc ggctcgaggc cgcaactccg ctgtcgatgg cggaaatcca
      781 ggccgacaag gaccagctgc gcgcggaatt cgcgatgtcg gcgcgacggc tcgaaatgaa
      841 cgtcgagcag ctcaagaaca agaccacgag ccagctcgcc gaactcggca agaagagcga
      901 cgcgatcaac cgcatgaaga tcgagctcgg cgaaaagaac gccacgatct tttcgctcga
      961 ggcgcgcgag aaggcagtga aggagcagct gcgcgccacc gaggaggaat tcgcggccaa
     1021 gaccgaactg ctgcgcggcg ccgaagcggc gctgaccgac aagcagggcg agctggcccg
     1081 gatcaatcac gaattgaacg atcgcgcgat gacggctgac agccgccagg tcgagctcgt
     1141 cctcggtcgc gcgcaactga tccttgatgc ctttgtcgcg cgcttcgagc gcgaagatgg
     1201 tcgcggcctt ttcgccgagc tcgatcttga gccggttgat gacgtcgctc ttcttgccga
     1261 gttcggcgag ctggccggtg ctcttgttct tcagctgatc gacgctcatt tcgagccgcc
     1321 gcgtcgacat cgcgaattcg gcgcgcaact ggtccttgtc ggcctggatt tccgccatcg
     1381 agagcggggt cgctgcctcg agccggcgca cggtcaagcg caccgcgcgg ccatgcacga
     1441 acggcacgat gatgagtccg agcaggctcg cggccaaaaa gccgagcccg aagaacatga
     1501 tcggttcgac catgaaagga agcctccgat cccgtccgct aagggatctt tccgacaatg
     1561 ccacgcgcga ccggccaact ccagcgcgcg cacgcgttaa cctaaattga cgagcgttcg
     1621 ctcgtcatca ccgggccggc gcgacgcgcc gtgacccggt gacctcgatc agcaggctcg
     1681 ttccccaagc cggatggccg ggcatcaggg
//
LOCUS       DATANS010000424         5222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_695420, whole genome shotgun sequence.
ACCESSION   DATANS010000424 DATANS010000000
VERSION     DATANS010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695420"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..796)
                     /locus_tag="VHT22_06165"
     CDS             complement(32..796)
                     /locus_tag="VHT22_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3137172.1"
                     /translation="MQSVFDLSGKVALVTGAYRGLGFAIAQGLARAGASVVLNGRRIE
                     AVEPAARALTAQSLKASTAIFDVTDRDAVRAAVTAIESEHGRIDVLVNNAGIQRRGAL
                     ADFSQQDWDAIIATNLTAPFVVSQAVLPGMIARKSGKIVHIASLMSEMARPSVVPYTA
                     AKGGVRQLTRGMAVELAPHNIQVNAIAPGYFATEMNRALIDNAEFNAWVCKRTPAGRW
                     GQPDELAGTAVFLASSASNYITGQLLIVDGGMSVAL"
     gene            complement(873..1649)
                     /locus_tag="VHT22_06170"
     CDS             complement(873..1649)
                     /locus_tag="VHT22_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3137173.1"
                     /translation="MDLAIRGLRVLITAGASGIGLATARAFAREGARVHVCDVDQAAL
                     KSVAASDPAIVWSVCDVADGKAIARMLDQVTATFGGLDALVNNAGIAGPTAACEDIAL
                     ADWERTLAVNLTGQFLCAQRAIPWLRKSSNPSIANLSSAAGRFGFPMRTPYAASKWAV
                     IGLSKSLAIELGRDGVRVNAICPGSVAGPRIDAVFANKAAARGVSAATVRDEALARTS
                     LRRLVSADDIANAIVFLASPLGANISGQALPIDADTQALF"
     gene            1843..3099
                     /locus_tag="VHT22_06175"
     CDS             1843..3099
                     /locus_tag="VHT22_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3137174.1"
                     /translation="MDRSPTSRRRRSLLKGAGAAAALGVAGFPAIIRAQSDAIRIGHL
                     TPRTGFLGPLGEYAVMGVNLAVDEINAAGGIMGRKLDLIAEDSVNPQTASTKAERLIE
                     RDKVVAIIGEISSASGLAIAQVAQRNRTLFFNTGCNSDELRGKSCNRYMFHTEAANSM
                     YVSACGQALLRDGMVKGKKWYSLTADYAFGHDLLKVAKRFMTANGGEFAADELVATDV
                     ADFSPYMLKIRNAKPDLVVSNLAGAQITNFMKQYLEFGLPFPVAGFGFDTAVAWGAGK
                     GNVLGIWPLVWDSQLKTPASQRFTDAFVKKYGKPPENQAWGDYMSTKHVAQAMNEIKS
                     TDSTKVVEHLEKGAKFDILKAREGYYRGWDHQLMQEMYTITFKKAADVKDRWDLFTLS
                     PPVPAASQSLEVIAPTREENNCTMPA"
     gene            3149..4021
                     /locus_tag="VHT22_06180"
     CDS             3149..4021
                     /locus_tag="VHT22_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX3137175.1"
                     /translation="MLIEVLEQVLNGLLAGAYYLLIALGLTLIFSLGGLVNLAHGAFY
                     ALGAYFAVKLADTLGFAGALVVSPLAVAGVGLVVERLLFRRFYREDPALGLLLTFGLA
                     LVAEQALRWIFGAAPIPFSIPPALKGQVFVGDFIYSKYRLLMLAIAAAAVAGLWLLLR
                     RTPFGRVVRAGVQNPDMVAALGISLAPYMASVVALAVALAALAGVLLAPISGVHPAMG
                     TDILTASFVVVVIGGLGSFWGVVWAALIVGVVRGLTVYFFPPAAEASMYLLMLLVLLF
                     RPRGLFGERIQRFE"
     gene            4018..>5222
                     /locus_tag="VHT22_06185"
     CDS             4018..>5222
                     /locus_tag="VHT22_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3137176.1"
                     /translation="MKLPRSPLAIAALVCAGLPFALLAGGLTLTSATDVVIFAIACMA
                     LNVLVGHTGLVSFGHGAWFGLGAYAAALAQRHWFPASVLWPALFALVFLLVASVLVGF
                     LVLRRRGVYFSLLTLAFSALTYAVAFRWTAFTGGENGLGGVSRATWLGVDLENPWVYY
                     AVVATLGLLVVHALSRFHASPIGSVLLAIRENEQRAQFIGYATQRYKQIAYAASATLT
                     GFAGVLFAFHHRFASADPTSISSSGELLAMVIIGGMRSLLGPALGALFYILFREYLSI
                     WTPNWLFFFGLLFVAFVVFSPTGLVGVWRRLTEPMRPRTVEAAAMADRSIEHGRPLPA
                     FLLPAARPAGTVLEAKGLAKAFGGIHAVEEASLAVADRTLHALIGPNGAGKTTLFNLV
                     SGMFMPDRGR"
BASE COUNT          814 a         1691 c         1715 g         1002 t
ORIGIN      
        1 ccaaaactgt gcttcgtgcg taacgggcta actaaagcgc tacgctcatt ccgccatcga
       61 cgatcagcag ctgcccggtg atgtagttcg atgcgctcga ggcgagaaat acggcggttc
      121 cggccagctc gtcgggctgg ccccagcggc ccgcgggcgt gcgcttgcac acccatgcgt
      181 tgaactcggc attgtcgatc agcgcgcgat tcatttcggt cgcaaaatag cccggggcga
      241 tcgcgttgac ctggatgttg tgcggtgcga gctcgacggc catgccgcgc gtcagctggc
      301 gcacgcctcc cttagctgcg gtgtacggca cgacgctcgg gcgcgccatc tcgctcatca
      361 gcgaggcgat gtgcacgatc ttgccgctct tgcgcgcgat catgcccggc agcacggcct
      421 gcgacacgac gaagggtgcg gtcagattgg tggcgatgat ggcgtcccaa tcctgctgtg
      481 aaaagtcggc cagcgcccct cggcgctgga tgcctgcatt gttgaccagc acgtcgatcc
      541 ggccgtgctc gctctcgatc gccgtcaccg cggctcgcac cgcatcacga tcggtgacgt
      601 cgaatatcgc ggtcgatgcc ttcagcgact gggccgtcaa cgcccgcgcc gccggttcga
      661 ccgcctcgat ccggcgcccg ttgaggacga ccgaggcccc ggcccgcgcg aggccttgcg
      721 cgatagcaaa gccgaggccg cgataggcgc cggtaaccag cgcgaccttg cccgacagat
      781 cgaacacgga ttgcatcggt tcgcctcctt ctaattacag cacgaggccg cctgcactcg
      841 gcgctcaccg cggccgatgc aaagcgagtg tctcagaaca gcgcttgcgt atcggcatcg
      901 atcggcaaag cctgtcccga aatgttggcg cccagaggcg aggcgagaaa aacgatcgcg
      961 ttggcgatgt cgtcggcgga taccagccgt ctgagtgatg tccgagcgag tgcttcgtcg
     1021 cgaaccgtcg cggcggagac gccgcgcgcg gcggctttgt tggcaaagac cgcatcgatg
     1081 cgcggaccgg cgaccgagcc gggacagatc gcgttgaccc ggacgccgtc gcggccgagc
     1141 tcgatcgcca gcgacttgga cagccctatc acggcccact tggacgcggc gtacggcgtg
     1201 cgcatcggga agccgaagcg gcctgcggcc gacgaaagat tggcgatgct gggattgctg
     1261 ctcttcctga gccacggaat ggcacgctgc gcgcacagga actgtccggt caggttgacc
     1321 gcgagcgtgc gttcccaatc ggccagcgcg atgtcttcgc aggccgcggt cggaccggcg
     1381 atgccggcgt tgttgaccag ggcgtcgagg ccgccgaagg tcgcggtcac ctggtcgagc
     1441 atccgcgcga tcgccttgcc gtcggcaacg tcgcagacac tccagacaat ggcggggtcg
     1501 cttgccgcga ccgactttaa cgccgcttgg tcgacgtcgc acacgtgaac tcgagcacct
     1561 tcgcgagcaa atgcccgcgc cgtcgcgaga ccgatgccgg acgcgccggc cgtgatgagg
     1621 acgcgcaatc cgcggatcgc gagatccatg ttcagctctc ctggaaacga agcgaatcgc
     1681 gcaatcgcgg accgctcgcg cgttggggtc gaggacgatc gcgcggcatg gacagcaata
     1741 gcgctttggg attataactg gcatcgcatt tcgccaagcg gtgccgaacg gccattgttt
     1801 aacgagccgt ggcccgcttt ctcgtcatcc cgaggaggag tcatggatcg ttcgccgaca
     1861 agccgtcgcc gtcgcagttt gctcaaggga gccggagcgg ccgccgccct gggcgtcgcc
     1921 ggatttcccg ccatcattcg cgcccagtcc gatgcgatac ggatcggcca cctgacgccg
     1981 cgcaccggct ttctcgggcc gctgggcgaa tacgcagtga tgggcgtgaa tctcgcggtc
     2041 gacgagatca atgccgccgg cgggatcatg ggccgcaagc tcgacctgat cgccgaagac
     2101 agcgtcaatc cgcagacggc atcgaccaag gccgagcgcc tgatcgagcg cgacaaggtc
     2161 gtcgcgatca tcggcgagat cagctcggcg tcgggactgg cgatagcgca ggtggcgcag
     2221 cgcaaccgaa cgctcttttt caacaccggt tgcaactccg acgaactgcg cggcaagtcg
     2281 tgcaaccgct acatgttcca caccgaggcc gccaacagca tgtacgtcag cgcctgcggg
     2341 caggccttgc tgcgcgacgg catggtcaag ggcaagaagt ggtacagcct gaccgcagac
     2401 tatgcgttcg ggcacgacct gctcaaggtg gccaagcgtt tcatgaccgc gaacggtgga
     2461 gagttcgccg ccgacgagct ggtcgcgacc gatgtcgccg atttctctcc gtacatgctc
     2521 aagatccgca acgccaagcc ggacctcgtg gtatcgaatc tcgccggcgc gcaaatcacc
     2581 aatttcatga aacagtatct cgaattcgga ttgccgtttc cggtcgccgg attcggcttc
     2641 gacactgccg tcgcgtgggg cgccggcaaa ggcaacgtgc tcggcatctg gccgctggtc
     2701 tgggactcgc agttgaagac gccggcgtcg cagcgattca ccgacgcgtt cgtgaaaaaa
     2761 tacggcaagc cgccggagaa ccaggcctgg ggcgactaca tgtcgaccaa gcacgtcgcg
     2821 caggcgatga acgagatcaa aagcactgat tcgaccaagg tcgtcgagca tctggagaag
     2881 ggcgccaagt tcgacatcct caaagcgcgc gaaggctatt accgcggctg ggaccatcag
     2941 ttgatgcagg agatgtatac gatcacattc aagaaggctg ccgacgtcaa ggatcgctgg
     3001 gatctgttca ccttgtctcc gccggtgccg gcggcgagcc agagcctcga agtgatcgcg
     3061 ccgacgcgcg aagagaacaa ctgcacgatg cccgcctgat caaaacggcg atgcgcgcgg
     3121 tgcgccgccg cgcgcggcgg cggtcacgat gctaatcgag gttctcgagc aggtattgaa
     3181 cggcctgctc gccggcgcct attacctgct gatcgcgctc ggcctgacgc tcattttctc
     3241 gctgggcggg ctggtcaacc tcgcgcacgg ggcgttctac gcactggggg cgtatttcgc
     3301 cgtcaagctc gccgacacgc tcggtttcgc cggcgcgctg gtcgtctcgc cgctggcagt
     3361 cgccggcgtc ggtctggtcg tcgagcgcct gctgttccgt cgcttttacc gcgaggatcc
     3421 cgcgctcggc ctgctgctga cctttggcct ggcgctggtc gccgagcagg cgctgcgctg
     3481 gatattcggt gcggcgccga ttccgttttc gattccgccg gcgctgaaag gtcaggtctt
     3541 cgtcggcgat ttcatctatt cgaagtaccg gctgttgatg ctcgccatcg ccgccgccgc
     3601 cgttgcgggg ctgtggctgc tgctgcggcg gacgccgttc ggaagagtcg tccgcgccgg
     3661 ggtgcaaaac cccgacatgg tcgccgcact cgggatttcg ctcgcgccgt acatggccag
     3721 cgtggttgcg ctggcagtcg ccctggctgc cttggccggc gtgctgctgg caccgatttc
     3781 cggtgtccat ccggcaatgg gcaccgatat cctgacggcg tccttcgtcg tcgtcgtcat
     3841 cggcgggctg ggctcgttct ggggtgtcgt ctgggcggcg ctgatcgtcg gtgtcgtgcg
     3901 cggcctgacc gtgtatttct ttcccccagc ggcggaagct tcgatgtacc tgctgatgct
     3961 gctggtgctg ctgttccggc cgcgcggctt gttcggcgaa cgcatccagc gcttcgaatg
     4021 aagctgccac gctcgccgct ggcaatcgcc gcactggtgt gcgcgggatt gccctttgcg
     4081 ctgctcgcag ggggcctaac cttgaccagc gcgaccgacg tggtgatctt cgccatcgca
     4141 tgcatggcgc tgaacgtcct ggtcggccat accggcctgg tttcgttcgg ccacggcgca
     4201 tggttcggcc tcggcgccta tgctgcggcg ctggcccagc gccattggtt tcccgcatcg
     4261 gttctctggc ccgcgctgtt cgcgctggtc ttcctgctcg tcgcctcggt gctcgtcggt
     4321 ttcctggtgc tgcgccgtcg cggcgtttac ttctcgctgc ttacgctggc attctcggcg
     4381 ctgacctatg cggtggcgtt tcgctggacc gcgttcaccg gcggcgagaa cggcctcggc
     4441 ggggtcagtc gcgcgacgtg gctcggcgtc gacctggaga acccgtgggt ctactacgcc
     4501 gtcgtcgcga cgctcggtct gctcgtcgtc catgcgctgt cgcgctttca cgcgtcaccg
     4561 atcggtagcg tcctgctcgc gattcgcgag aacgagcaac gcgcgcagtt catcggctat
     4621 gcgacgcagc ggtacaagca gatcgcctat gcggcatcgg cgacgctgac cggctttgcc
     4681 ggtgtcctgt ttgcgtttca ccatcgcttc gcctcggccg atccgacgtc gatctcctcg
     4741 tccggcgagt tgctggccat ggtgatcatc ggcggcatga gaagcctgct gggcccggcg
     4801 ctcggggcgt tgttctacat tctctttcgc gaatacctgt cgatctggac gccgaactgg
     4861 ttgttttttt tcggcctgtt gttcgtcgcc tttgtcgtgt tctcgccgac cggcctggtc
     4921 ggcgtgtggc ggcgtctcac cgagccgatg cgtccccgca ccgtcgaggc tgccgcgatg
     4981 gcggatcgct cgatcgagca tgggcgaccg ctgccggcgt ttcttttgcc ggccgcgcgt
     5041 ccggcgggga ccgtgctcga ggccaagggg ctggcgaagg cattcggcgg tattcatgcc
     5101 gtcgaggaag caagccttgc agtggccgat cgcacgctcc acgcgttgat cggacccaac
     5161 ggcgccggca agacgacgct gttcaatctg gtttccggga tgttcatgcc cgatcgcggc
     5221 cg
//
LOCUS       DATANS010000425         2036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_817883, whole genome shotgun sequence.
ACCESSION   DATANS010000425 DATANS010000000
VERSION     DATANS010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817883"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VHT22_06190"
     CDS             <1..367
                     /locus_tag="VHT22_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX3137177.1"
                     /translation="SAIMVAAIGGNLAIVKKLYARGAEINHPGWTPLIYAATGGHADV
                     ARYLIDAGANVNAASPNGTTALMMAVREGHAAVVDLLLERGADPGHRNENGATALAWA
                     KRGGFDAIERALRRRGAKD"
     gene            complement(389..1036)
                     /locus_tag="VHT22_06195"
     CDS             complement(389..1036)
                     /locus_tag="VHT22_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX3137178.1"
                     /translation="MQPDPDTLPKARATVTALRHAAIVALAAIALVLLPSAGLAAQPA
                     TTSGHARAVERPVARVLQSAQDLAIYALGLIGVDYRYGGSSPEQGLDCSGLVRYVFQQ
                     VVGVTLPRTAGEMARLGGRVAPGDLQAGDLVFFNTRSSPFSHVGIYLGDDRFIHAPRR
                     GGEVEIVALSQRYWQQRYDGARRLVAFVPELISSAQAAPAGPAPVRADAVADSHR"
     gene            complement(1088..1375)
                     /locus_tag="VHT22_06200"
     CDS             complement(1088..1375)
                     /locus_tag="VHT22_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEX3137179.1"
                     /translation="MKRIAVTVVYALPAGATQIELSLPSGASVAEALALSGIAGRHPE
                     IDLAAMAVGVYGVRVALDTRLSNGDRVEIYRPLQADPKDLRRRRAIRRGAD"
     gene            complement(1372..1812)
                     /locus_tag="VHT22_06205"
     CDS             complement(1372..1812)
                     /locus_tag="VHT22_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEX3137180.1"
                     /translation="MRSVHKSVLVPYSAQQMFDLVERVEDYPKFLPWCGGARILQQTT
                     EETLAQIDIDYRGVRAHFTTANRNQPPDRIVISLRDGPFRRLDGIWQFRALSDSACKV
                     EFELNYEFATAMLEGLIGPVFGRIADSFIDAFVKRAVAVHGRGA"
     gene            1851..>2036
                     /locus_tag="VHT22_06210"
     CDS             1851..>2036
                     /locus_tag="VHT22_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HEX3137181.1"
                     /translation="MSIVENKKAYHDYFVEEKFEAGLALEGWEVKAIRAGRAQLKEAY
                     VVVKGGEIVLLGAHVTPL"
BASE COUNT          365 a          643 c          705 g          323 t
ORIGIN      
        1 cagcgcgatc atggtcgctg cgataggcgg aaacctggcg atcgtgaaaa agctctacgc
       61 tcgcggcgcc gaaatcaatc accccggatg gacgccgctg atctacgccg caaccggcgg
      121 tcatgccgac gtcgcccgct atctgatcga tgccggcgcc aacgtcaatg cggcatcgcc
      181 gaacggcacg acggctctga tgatggctgt gcgcgagggg catgccgcgg tggtcgactt
      241 gctgctggaa aggggagccg accccggaca tcgcaacgag aacggtgcga cggccttggc
      301 atgggccaag cgcggcgggt tcgatgcgat cgaacgcgcg ttgcggcggc ggggggctaa
      361 ggactagaga gcggcgttgt gcggtgcttc aacgatgcga atcggcgacg gcgtccgcac
      421 gcaccggcgc agggcctgcc ggcgcggcct gcgccgagct tatcaactcg ggcacaaacg
      481 cgactagtcg gcgcgcgccg tcgtagcgct gctgccaata gcgctgcgac aacgcgacga
      541 tctcgacctc tccgccacga cgcggagcgt ggatgaagcg atcgtcgccg aggtaaatgc
      601 cgacatgcga aaacggcgag cttcgggtgt tgaagaacac gaggtcgccg gcctggagat
      661 cgccaggggc gacccggccg cccagccgtg ccatttcccc cgccgtgcgc ggcagagtca
      721 cgccgacgac ctgctgaaac acgtagcgga ccaggccgct gcaatcgaga ccctgctcgg
      781 ggctgctgcc gccgtagcga tagtcgacgc cgatcagacc caaggcgtag atcgcgaggt
      841 cctgggcgct ttgtagcacg cgagccaccg gacgctcgac ggcgcgggcg tgaccggagg
      901 tggtggcggg ctgcgctgcc agcccggcgc ttggcagcag caccagcgct atcgcggcca
      961 gcgcaacgat cgcggcgtgg cgcagcgcgg tcaccgttgc gcgagctttg ggcaaggtat
     1021 cggggtctgg ttgcattttg tacgttaatt cagcgacttg ccaccatacc cgggtcggcg
     1081 gaccgagtca atcggcgccg cggcggatgg cgcgccggcg ccggaggtcc ttcgggtcgg
     1141 cctgcagcgg ccggtagatt tcgactcggt cgccgttcga cagcctggtg tcgagcgcga
     1201 ctctcacgcc atacacgccg accgccatcg cggcgaggtc gatttccggg tggcgtcccg
     1261 ctattcccga cagcgccagt gcctcggcaa cgctggcgcc ggacggcagc gagagctcga
     1321 tttgggtggc gcccgcgggc agcgcgtaga cgacggtgac ggcgatccgc ttcacgcgcc
     1381 gcggccgtgg accgccaccg cgcgcttgac gaaggcgtcg atgaagctgt cggcaatgcg
     1441 gccgaacacc ggcccgatca atccttccag catcgccgtc gcaaattcgt agttgagctc
     1501 gaattcgacc ttgcatgccg aatcgctcaa cgcgcgaaac tgccatatgc cgtcgaggcg
     1561 gcgaaacggg ccgtcgcgca gcgagatgac gatccggtcg ggcggctggt tgcgattggc
     1621 ggtggtgaag tgcgcgcgca cgccccggta atcgatgtcg atctgagcca gcgtctcttc
     1681 ggtggtctgc tgcaggatcc tggctccccc gcaccatggc aggaacttcg gatagtcctc
     1741 gacgcgctcg acgagatcga acatctgttg cgcgctgtac ggcaccagca ccgacttgtg
     1801 tacgctgcgc attgctacaa tacgaaactg gaagtgacga gcacgtcggt atgagcatcg
     1861 tagaaaacaa aaaagcctat cacgactatt tcgtcgagga gaaattcgag gcgggcctcg
     1921 cgctggaagg ctgggaggtc aaggcgatcc gcgccgggcg cgcacagctg aaggaagcct
     1981 acgtcgtcgt caagggcggc gagatcgttc tgctcggcgc gcatgtgacg ccgttg
//
LOCUS       DATANS010000426         2511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_484387, whole genome shotgun sequence.
ACCESSION   DATANS010000426 DATANS010000000
VERSION     DATANS010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484387"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..>2511)
                     /gene="ppc"
                     /locus_tag="VHT22_06215"
     CDS             complement(178..>2511)
                     /gene="ppc"
                     /locus_tag="VHT22_06215"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX3137182.1"
                     /translation="PVLTAHPTEVQRKSILDAEREIARLLQWRDRSTLTPEEDEAMRT
                     GLYRQVLSLWQTAMVRLTTLQVSDEIDNGLAYYRYTFLDEIPRLYTALEDRFAREYGT
                     ALAAPPLLRMGSWIGGDRDGNPFVVAETLQYASRAQASLVLLHHLAEVHQLGAELSLS
                     NRLVTPTERLLALAAAAHDPNPHRGDEPYRQALTGIYARLAATARELADCAPPRAPHV
                     ALPSYRAPDEFLADLDVIAESLATHGAEQLAAGRLRLLRRAVSVFGFHLAALDLRQNS
                     DVHEQVVGELLARAGVHSDYAALDEAQRVRLLVAELGSPRLLDTPHVTRSPMLQSEIA
                     ILHAAADIHQRFGPAALPNYVISKCQSLSDLLEVAIMLKEVGLLTPNRLAVNIVPLFE
                     TIADLERCDEVMASAFRLPLYQRWLASRGGWQEVMLGYSDSNKDGGFVTANWALYQAE
                     LKLVDAFRSFDVKLRLFHGRGGTVGRGGGPSYEAILAQPSGSVNGAIRVTEQGEIIAS
                     KYSDPELGRRNLETLVAATLEATLVRDRSETDHSQDFHDAMHALSGFAFKAYRALVYE
                     TPEFLAYFRASTPIAEIAELNLGSRPASRRPSQRIEDLRAIPWVFSWSQCRLTLPGWY
                     GFGTAVEAWLDEEPATRDERIALLRDMHRRWPFFRSMLSNMSMVLAKTDLAIASRYAD
                     LVTDPAVREAIYPTLVAEHRRTMHWLLQVTGQSGLLDDNPTLARSIRNRFPYLDPLNH
                     VQVELLRRYRAGQTDIRTKRGIHLTINGLAAGLRNSG"
BASE COUNT          434 a          810 c          840 g          427 t
ORIGIN      
        1 cggtgtccag attgagaaaa agtctttgtc ctgattcatg ttttgttgca ctccagatga
       61 ggattttgct gccggaaccc gggcatacca ggttcgacag cggaattgcc ggcgggttgc
      121 gaacgcagag gcgctccggg ccgggcaggc gccggccgtc ggcccgtggc gcggcggtca
      181 gccactgtta cgcaagcccg cggccagtcc gttgatcgtc agatggatgc cgcgcttggt
      241 gcggatgtcg gtctggccgg cgcgataacg gcgcaaaagc tcgacctgaa catgattgag
      301 cggatcgagg tacggaaacc ggttgcggat gctgcgcgcc aacgtcggat tgtcgtcgag
      361 cagtccgctc tggccggtca cctgcagcag ccagtgcatg gtgcgcctgt gctcggccac
      421 tagtgtcggg tagattgctt cgcgcaccgc cggatcggtg accaggtccg cgtaacgcga
      481 cgcgatcgcg aggtcggtct tcgcgagcac catgctcatg ttcgacagca tgctgcggaa
      541 aaacggccag cgccggtgca tgtcgcgcag cagcgcaatg cgctcgtcgc gcgtcgcggg
      601 ctcttcgtcc agccaagcct cgaccgcggt gccgaaaccg taccagccgg gcaaggtcag
      661 ccggcactga ctccagctga atacccaggg aatcgcgcgc aggtcttcga tgcgctgcga
      721 cggccggcgc gacgcgggcc ggctgccgag attcagctcg gcgatttcag cgatcggggt
      781 cgaggcgcgg aaatacgcca gaaactctgg cgtttcgtag accagcgcgc ggtacgcctt
      841 gaacgcaaat ccggacagcg cgtgcatcgc atcgtgaaaa tcctgggagt gatcggtttc
      901 actgcgatcg cgcaccagcg tcgcctcgag cgtcgcggcg accagggttt cgagattgcg
      961 ccgtccgagc tccggatcgg aatacttgct ggcgatgatc tcgccctgct cggtgactcg
     1021 gatcgcgcca ttcacgctgc cggacggctg cgccagtatc gcttcgtagc tcggtccgcc
     1081 gccgcggccg accgttcccc cgcggccgtg gaacaggcgc agcttgacgt cgaagctgcg
     1141 aaacgcatcg accagcttga gctcggcctg gtacagcgcc cagttcgccg tcacgaagcc
     1201 gccatccttg ttgctgtccg aatagccgag catcacttcc tgccagccgc cgcggctcgc
     1261 cagccagcgc tgatacaagg gcagtcgaaa cgccgaagcc atcacttcgt cgcagcgctc
     1321 gagatcggct atcgtttcga acagcggaac gatattgacc gcgagccggt tcggcgtcag
     1381 caagccgact tccttcagca tgatggcaac ttcaagcaag tcggagagcg actggcattt
     1441 ggagatcacg taattgggca gcgctgccgg gccgaagcgc tggtggatgt cggctgccgc
     1501 atgcagaatc gcaatctcgc tctgcagcat cggcgagcgc gtcacatgcg gcgtgtcgag
     1561 caggcgcggg ctgccgagct cggcgacgag cagacggact cgctgcgctt cgtccagcgc
     1621 agcatagtcg gaatggacgc cggctcgcgc caggagctcg ccgacgacct gttcgtgcac
     1681 gtccgaattc tggcgcagat ccagcgccgc cagatgaaaa ccgaatacgc tgacggcccg
     1741 ccgaagcagc cgcagacggc cagccgccag ctgctcggcg ccgtgcgtcg ccagcgactc
     1801 ggcgatcaca tcgaggtcgg cgaggaattc atctggtgcc cggtaggatg gcagcgcgac
     1861 gtgcggcgca cgcggcggcg cgcagtcggc aagctcgcgc gcggtcgccg cgagcctcgc
     1921 atagattccc gtcagcgcct gccggtacgg ttcgtcgccg cgatgcggat tcggatcatg
     1981 cgcggcggcc gcaagtgcga gcagccgctc ggtgggggtt accaggcgat tcgacagcga
     2041 cagctcggcg ccgagctggt ggacctcggc caggtgatgc agcaggacca gcgacgcctg
     2101 ggcgcggctc gcgtattgca gcgtctcggc gaccacgaac gggttgccat cgcgatcacc
     2161 gccgatccac gagcccatcc gcaacagcgg cggcgccgcg agcgcggtcc catactcgcg
     2221 cgcgaagcga tcttcgagcg ccgtgtacag cctcggaatc tcgtcgagaa aagtgtaacg
     2281 gtagtaagcg agcccgttgt cgatctcatc gctgacttgc agcgtggtca ggcggaccat
     2341 cgccgtctgc catagcgaca gcacctggcg atagagtccg gtgcgcatcg cctcgtcctc
     2401 ttcgggcgtc agcgtgctgc ggtctcgcca ttgcagcagc cgcgcgatct cgcgctcggc
     2461 gtcgagaatg ctcttgcgct gaacttcggt cgggtgcgcg gtaagcaccg g
//
LOCUS       DATANS010000427         1671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_484409, whole genome shotgun sequence.
ACCESSION   DATANS010000427 DATANS010000000
VERSION     DATANS010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484409"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="VHT22_06220"
     CDS             <1..152
                     /locus_tag="VHT22_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEX3137183.1"
                     /translation="ALAMNDIGSVRLALTRPAFVDRYQESRATGSFILIDDATHNTVA
                     AGMVQ"
     gene            149..961
                     /gene="cobA"
                     /locus_tag="VHT22_06225"
     CDS             149..961
                     /gene="cobA"
                     /locus_tag="VHT22_06225"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX3137184.1"
                     /translation="MNTRSGIVYLVGAGPGAPDLLTLRAARLLERADIVFYDALVHPD
                     TIALAANARKIAVGKRCGKHSTAQQFINKRLVDAAGAHAVVVRLKGGDPMLFGRAQEE
                     IAALEAAGVRYEVVPGITAALAASAQIGISLTQRGLVRTVSLVTPRVGEGEAANEWAR
                     SIAAADAGAIYMGVGQADAIAAALIAAGKPGATPLAVVENASLPNARVIYSTIAALPR
                     IADADLTGPAVILIGAQFRAYHAAAANAPADIATEASDRRESDDLGRVRTGR"
     gene            1001..>1671
                     /locus_tag="VHT22_06230"
     CDS             1001..>1671
                     /locus_tag="VHT22_06230"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX3137185.1"
                     /translation="MTEFAWLALSGLGVGLMVGMTGVGGGSLMTPLLIAAFGIPAPIA
                     VGTDLACAAITKTAATFAHRAAGTIKVPVVVLLVAGSGPAAIATLVVLANVSVTPQGL
                     ARTIRLSVAIAVLISVALLLVRGSIRRWSLHSAALARLRRHRPAATVVAGALIGAAVA
                     LTSLGAGAIGAAILALLYPELEPAEIAGTDIAHAVPLTAIAAAGHAWLGNVDAGLLLA
                     LVTGGL"
BASE COUNT          234 a          580 c          592 g          265 t
ORIGIN      
        1 cggcgctggc gatgaacgac atcggcagcg tccgtcttgc gctgacgcgg ccggcattcg
       61 tcgaccgcta ccaggagagc cgtgccaccg gaagcttcat cctgatcgac gacgcgacgc
      121 acaacacggt agccgccggg atggtccaat gaacacgcgc agcggcatcg tctacctggt
      181 cggcgccggc cctggcgccc cggacctgct gaccttgcgc gctgcgcggc tgctcgagcg
      241 cgccgacatc gtgttctacg atgcgctggt gcatccggac acgatcgcgc tcgccgcgaa
      301 cgctcgcaag atcgcggtcg gcaagcgttg cggcaagcat tcgacggcgc agcaattcat
      361 caacaagcgg ctggtcgatg cggcgggcgc gcatgcggtc gtcgtgcggc tcaagggcgg
      421 cgatccgatg ctcttcggcc gcgcacagga ggaaatcgcc gcgctcgagg cggccggcgt
      481 gcgctacgag gtggtgccgg gcattacggc ggcgctggcg gcgagtgcgc aaatcgggat
      541 ttctttgacg cagcgcggcc tggtgcgcac agtcagcctg gtcaccccgc gcgtcggcga
      601 gggcgaggct gcaaacgaat gggcgcgaag catcgccgcc gccgatgccg gcgccatcta
      661 tatgggcgtc ggtcaggcgg acgccatcgc ggcggcgctg atcgccgccg gcaagccggg
      721 agcgacgccg ctggcagtgg tcgagaacgc gtcgctgccg aacgcgcgcg tcatctattc
      781 gaccatcgcg gcgttgccgc gcatcgccga tgccgatctg accgggcccg ccgtgatcct
      841 gatcggagca caatttcgcg cttatcacgc tgcggccgca aacgcgccgg ccgacatcgc
      901 caccgaggcg agcgaccgtc gcgagagcga tgatctcggc cgcgtccgca ccggccgcta
      961 ggcagccggc gaccgcgacg ccatttcagg attggtcgcg atgaccgaat tcgcgtggct
     1021 agcgttgtcc ggtctgggcg tcgggctgat ggtcggcatg accggcgtcg gtggcgggtc
     1081 gctgatgacg ccgctgctga tcgccgcatt cggcattccg gcccccatcg cggtcggtac
     1141 cgatctcgcc tgcgcggcca taaccaagac cgctgcgacg ttcgcgcatc gcgccgcggg
     1201 cacgataaag gtacccgttg tcgtcctgct cgtggccggc agcgggcccg cggcgatcgc
     1261 gacgctcgtg gtccttgcga acgtcagtgt cacgccgcaa ggattggcgc gaacgatccg
     1321 cctgtcggtg gctatcgcgg tgctgatcag cgtcgcgctg ttgctggttc gcgggagtat
     1381 tcggcgctgg tcgctccatt ccgcggcgct tgcccgcctg cgccgccacc gcccggcggc
     1441 gacggtcgtc gcgggggccc tgatcggcgc cgccgtggca ctgacgtcgc tcggcgcggg
     1501 agcgataggc gcggcgatcc tcgcgttgct ctaccccgag ctcgagcctg ccgagatcgc
     1561 gggcactgac atcgcgcacg ccgtcccgct gacggcgatc gcggcagcag gtcacgcctg
     1621 gctcggcaac gtcgacgccg gcctgctgct cgccctggtc actggcggcc t
//
LOCUS       DATANS010000428         6119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_695568, whole genome shotgun sequence.
ACCESSION   DATANS010000428 DATANS010000000
VERSION     DATANS010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695568"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VHT22_06235"
     CDS             complement(<1..548)
                     /locus_tag="VHT22_06235"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292126.1"
                     /note="Catalyzes the reversible hydrolysis of the amide
                     bond within dihydroorotate. This metabolic intermediate is
                     required for the biosynthesis of pyrimidine nucleotides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX3137186.1"
                     /translation="MTATNVEIRNGRLIDPRHGVDGKASLFVAAGRVAGVGSPPAGWR
                     AGRIIDAAGCVVCPGLIDLAARLREPGFEYMATLESEMNAAIAGGVTSLACPPDTDPS
                     LDEPGLVEMLKYRARSLNQAHVYPVGALTVGLKGENITEMGELAEAGCVAFSHADAPL
                     ADTQVLRRALQYAATFGYAVWLR"
     gene            complement(563..1522)
                     /locus_tag="VHT22_06240"
     CDS             complement(563..1522)
                     /locus_tag="VHT22_06240"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX3137187.1"
                     /translation="MRNPQLNGDGQLQHLLTLEGLPVTVIDRILDTALPFVSIAERDV
                     KKVPLLRGKTVFNLFFENSTRTRTTFEIAAKRLSADVINLNIGVSSTSKGETLLDTID
                     NLVAMNADMFVVRHAQSGAAHLIAQHLSAADRPHIHVINAGDGRHAHPTQGLLDLFTI
                     RHYKKDFHGLTVAIVGDLLHSRVARSLIHGLTTLGTTEVRVIGPQTLLPAAIESMGVR
                     VFHDMRAGLAGADVVVMLRLQSERMKGALLPSAGEYFKHYGLTTEKLALAKPDAIVMH
                     PGPMNRGVEIDSAVADGAHSVILPQVTFGIAVRMAVMSMLAGN"
     gene            complement(1515..1979)
                     /gene="pyrR"
                     /locus_tag="VHT22_06245"
     CDS             complement(1515..1979)
                     /gene="pyrR"
                     /locus_tag="VHT22_06245"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX3137188.1"
                     /translation="MGAAIAYDAKLVGIYSGGAWLAERLAEILPGEHPVGFIDVSFYR
                     DDFGKKGLRAKVATTTLPFEVGNGRIVLVDDVLYTGRSVRAAINELFDYGRPESIELA
                     VLIDRGGRELPIEAAYVGARVAVARDSSIVLQRHLDGRFELSVESIESAPGA"
     gene            complement(2038..2496)
                     /gene="ruvX"
                     /locus_tag="VHT22_06250"
     CDS             complement(2038..2496)
                     /gene="ruvX"
                     /locus_tag="VHT22_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX3137189.1"
                     /translation="MSLAATLLRPAARAGTILAFDLGTRRIGVAVGETTLGLAHPLAT
                     IAADRDDDRLAAIASLVREWQPALLVVGLPTHADGTPHAMTARAQEFARRLEARFRVA
                     VELVDERYTTEAASAALSDAGVGERGHKPLRDQVAAQIILQAYFDQRTRT"
     gene            complement(2493..3059)
                     /locus_tag="VHT22_06255"
     CDS             complement(2493..3059)
                     /locus_tag="VHT22_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX3137190.1"
                     /translation="MGESRVNLTHHFLIAMPSMADPHFAHTLIYVCEHNEKGALGIVI
                     NKPIDMTLSSLFEQIQVPLGDSDLRAAPVHYGGPVQIDRGFVLHRPLGSWQSTLAISD
                     DLGLTTSKDILEAVGHGEGPHDVLVSLGYAGWSADQLEQEIAQNAWLTVEADPDVMFA
                     LPAETRLSAAMKLLGIDFSRLSDAVGHA"
     gene            complement(3141..5192)
                     /gene="fusA"
                     /locus_tag="VHT22_06260"
     CDS             complement(3141..5192)
                     /gene="fusA"
                     /locus_tag="VHT22_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX3137191.1"
                     /translation="MAKFATPNIRTLALVGHGASGKTTLVEALLHKAGAIQSVGSVER
                     GTTVSDFEALERSWQHSLRSSVLHLETQDTRVHIIDTPGFPDFIGQSIGALDAVETAA
                     IVINAAAGIEMIASRMMEWAAARRLCRLLIVNKIDAENVDLPAILAAIRATFGKECLP
                     INLPAGNGKRVVDCFFNPAGEADFSSVADAHRALVDQVVEVDEELMSLYLDQGEIAPE
                     QLHAPFEKALREGHLVPVCFVSAKNGAGVGELLDIFAKLLPNPTEGNPPLFTKGEGES
                     AVEFHSEPDPKKHVLAHVFKVVMDPFVGKLGIFRVYQGTVTRDTSLFVGDGRKPFKVG
                     HLFMLQGGKNVEVDAAVPGDIAAVAKVDEIEFDRVLHDSHDEDHIHMQPLAFPTPMQG
                     VALRPKKRGDEQRIWEVLHRMTAEDPTLVVEHDPHSNETVLRALGELHLRSVLERMST
                     QFKLEIETRPPRIPYRETITAQADGFHRHKKQTGGAGQFGEVSLRVEPMARGTGFEFV
                     DSTKGGVIPHQFMPAVRKGVEQVLGSGAIAGFPMHDVRVVVYDGKHHPVDSKEVAFVA
                     AGRKAFLDAIDKARPIVLEPIVTVEVICPDVKTGDIAGDLSSRRGQVTGTKGLQPGIL
                     AVSGLAPLAELEGYSARLKSVTGGQGSWTMQLSHYEPAPPNLQQQLATEYKKSRKQEE
                     D"
     gene            complement(5408..>6119)
                     /locus_tag="VHT22_06265"
     CDS             complement(5408..>6119)
                     /locus_tag="VHT22_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3137192.1"
                     /translation="FHYLPRLLGDAAHAPETARHNSVLLLRAGQGVAAVHVDEMIGNQ
                     EVVVKNIGPQLARVSGISGATVLGNGEIVLIINPVQLAQRLDTAAEAPMVVKTPQAAA
                     AAAAAAAAAGPPLIMIVDDSLTVRKITSRLLTREGYMVIAAKDGLDALQLLGERTPDA
                     ILLDIEMPRMDGFEFAKTIKADHNLVDIPIIMITSRTAEKHRNRAAELGIDLYLGKPY
                     QEEELLRNLRELLAVHAN"
BASE COUNT         1024 a         2031 c         1978 g         1086 t
ORIGIN      
        1 cgaagccaga ccgcgtagcc gaacgtcgcc gcgtactgaa gcgctcggcg cagcacctgt
       61 gtatcggcga gcggcgcgtc ggcatgcgag aaggcgacgc agccggcttc ggccagctcg
      121 cccatttcgg tgatgttctc tcccttgagt ccgacggtca gcgcaccgac cggatagacg
      181 tgtgcctgat tgagcgatcg cgcgcggtac ttgagcattt cgaccagccc gggctcgtcc
      241 agcgacgggt cggtgtctgg cggacaggcg agactggtca cacccccggc gatcgcggcg
      301 ttcatttccg attcgagcgt ggccatgtat tcgaatcccg gctcgcgcag gcgcgccgcg
      361 agatcgatca ggcccgggca gacgacgcat ccggccgcat cgatgattct gccggcgcgc
      421 cagccggcgg gcgggctgcc gacgccggcg acccttcctg cagcgacgaa cagcgaggcc
      481 ttgccatcga cgccatgccg cgggtcgatc agacgtccgt tcctgatctc gacgttcgtc
      541 gcggtcatcg tcgtggcacc gctcaattgc ccgccagcat gctcatcacc gccatccgca
      601 ccgcaatgcc gaacgtcacc tgcggcagga tcaccgaatg cgcaccgtcg gcgaccgccg
      661 agtcgatctc gacgccgcga ttcatcgggc ccgggtgcat gacgatcgcg tcaggcttgg
      721 ccaacgccag cttttcggtc gtcaatccgt aatgcttgaa gtattcgccc gccgacggca
      781 gcagcgcgcc tttcatgcgc tcgctctgca ggcgcagcat gacgaccacg tcggcgccgg
      841 ccaggccggc acgcatgtca tggaacacgc gcacacccat gctctcgatc gcagccggca
      901 gcagcgtctg cggcccgatc acccgaactt cggtcgtgcc cagcgtcgtc aggccgtgga
      961 tcagcgagcg cgcgacgcgc gaatgcagca ggtcgccgac gatcgcgacc gtcaggccgt
     1021 gaaaatcctt cttgtaatga cggatcgtga acagatcgag caggccctgg gtcggatgcg
     1081 cgtggcgacc gtcgccggca ttgatcacgt ggatatgtgg ccggtctgcc gccgaaagat
     1141 gctgggcgat caagtgcgcg gcaccgcttt gcgcatggcg cacgacgaac atgtcggcgt
     1201 tcatcgcgac caggttgtcg atcgtgtcga gcagcgtctc gcccttgctg gtcgatgaca
     1261 cgccgatgtt caggttgatc acgtcggccg acaatcgctt ggcggcgatc tcgaaggtcg
     1321 ttcgcgtacg cgtactgttc tcgaagaaca ggttgaagac ggtcttgccg cgcagcagcg
     1381 gcaccttctt gacgtcgcgc tccgcgatcg acacgaacgg cagcgcggta tcgagtatgc
     1441 gatcgatgac ggtgacgggc agcccttcca gcgtcagcag gtgctgcaac tggccgtcgc
     1501 cattgagctg cgggttacgc accaggcgcg ctttcgatcg actccaccga caattcgaag
     1561 cggccgtcca gatggcgctg cagcacgatc gacgaatcgc gtgcgaccgc gacccgggca
     1621 ccgacgtaag cggcttcgat cggcaactcg cgcccgccgc ggtcgatcag gaccgcgagc
     1681 tcgatcgact ccggccgccc atagtcgaac agctcgttga tcgccgcccg gaccgagcga
     1741 ccggtataga gcacgtcatc gaccagcacg atgcggccgt tgccgacttc gaacggcaag
     1801 gtcgtggtgg cgaccttggc gcgcagcccc ttcttgccga agtcgtcgcg gtagaacgag
     1861 acgtcgatga agccgacggg gtgctcgccc ggcagtatct cggcaaggcg ctccgcaagc
     1921 catgcgcctc cggaatagat gcccaccagc ttggcgtcgt aggcaatagc ggcgcccatt
     1981 ttctccgcca gggcggcgat agtcgcttcg gcatccggca ggccgtcgcc gatcgggtca
     2041 ggtgcgggtg cgctgatcaa aatatgcctg caggatgatt tgcgccgcga cctggtcgcg
     2101 cagcggcttg tgcccgcgct cgccgacgcc cgcatcggag agtgcggccg acgcggcttc
     2161 agtcgtgtaa cgctcgtcga cgagctcgac cgcgacccga aaacgagcct cgagtcgtcg
     2221 cgcgaattcc tgcgcgcgcg ccgtcatcgc gtgcggcgtt ccgtcggcgt gcgtcggcaa
     2281 gccgacgacc agcagcgccg gttgccattc gcgaaccagc gatgcgatcg cggcaagccg
     2341 gtcgtcatcg cgatccgcag cgatcgtggc cagcggatgc gcgagcccga gcgtcgtctc
     2401 gccgaccgcg acgccaatgc gccgggtgcc caaatcgaat gcgagtatgg tgccggcgcg
     2461 cgcagcgggc cgcagcaagg tcgccgccaa gctcacgcgt gcccgacggc gtcggacagc
     2521 ctggaaaaat cgattccgag cagcttcatc gccgccgaca gccgcgtttc ggccggcagg
     2581 gcgaacatca cgtcgggatc cgcctcgacg gtaagccagg cattctgcgc gatttcctgc
     2641 tcgagctggt cggccgacca acccgcatag ccgagcgaga caagtacatc gtgcgggcct
     2701 tcgccatgac cgacggcttc gaggatgtcc ttcgacgtcg taaggcccag gtcgtcgctg
     2761 atcgccagcg tcgactgcca gctgccgagc ggccggtgca ggacgaaacc gcggtcgatc
     2821 tggaccggac cgccgtaatg cacaggcgcg gcccgaagat cggaatcgcc gagcggcacc
     2881 tggatttgct cgaacagcga ggaaagcgtc atgtcgatcg gcttgttgat cacgatgccg
     2941 agcgcgcctt tttcgttgtg ctcgcagaca tagatcaatg tatgcgcgaa atggggatcg
     3001 gccatgctgg gcatggcgat caggaaatgg tgggtcagat tgacccgcga ttcacccatg
     3061 cggctgccag tgcgggatag cccatgaacg caattgtagt cgccgccgcc cgctggcaca
     3121 aatcccgggc cgaccgtcgg tcagtcttct tcctgcttgc gcgacttctt gtattcggtc
     3181 gccagctgct gctgcagatt gggcggcgcc ggctcgtagt gcgacaactg catcgtccag
     3241 cttccctgcc ccccggtcac cgatttcagg cgcgccgaat agccttcgag ctctgccagc
     3301 ggcgccagcc cgctgacggc gaggatgccc ggctgcagcc ccttggtccc ggtaacctgg
     3361 ccgcggcgcg acgacagatc tcccgcgata tcgccggtct tcacgtcggg acagatcact
     3421 tcgacggtga cgatcggctc gagcacgatc ggccgcgcct tgtcgatggc gtcgagaaaa
     3481 gccttgcggc cggccgcgac gaacgcgact tccttcgaat cgaccgggtg gtgcttgccg
     3541 tcgtagacga cgacgcgcac gtcgtgcatc ggaaatccgg caatcgcgcc gctgccgagc
     3601 acctgctcta cgcccttgcg caccgcaggc ataaactggt gtggaatcac gccgcccttg
     3661 gtcgaatcga cgaattcgaa tccggtgcct cgcgccatcg gctcgacacg cagcgagacc
     3721 tcgccgaact gtccggcccc gcccgtctgc ttcttgtgcc gatggaagcc atcggcctgc
     3781 gcggtgatcg tctcgcgata gggaatgcgc ggcggccgcg tctcgatttc gagcttgaac
     3841 tgggtcgaca tgcgttcgag caccgagcgc agatgcagct cgcccagcgc ccggagcacc
     3901 gtctcgttgc tgtgcggatc gtgctcgacg accagcgtcg gatcctcggc ggtcatccgg
     3961 tgcagcactt cccagatccg ctgctcgtcg ccgcgctttt tcgggcggag tgctacgccc
     4021 tgcatcgggg tcggaaaggc gagcggctgc atgtgaatgt gatcctcgtc gtgcgaatcg
     4081 tgcagcacgc ggtcgaattc gatctcgtcg accttggcga ccgccgcgat gtcgcccggc
     4141 accgcggcgt cgacctcgac attcttcccg ccctgaagca tgaacaaatg gccgaccttg
     4201 aacggcttgc gcccgtcgcc gacgaaaagc gaagtgtctc gggtcaccgt tccctgatag
     4261 acgcggaaaa tgccgagctt gccgacgaac gggtccataa cgaccttgaa gacgtgcgcc
     4321 agcacgtgct ttttcgggtc gggctccgag tgaaattcca cggcggattc gccctcaccc
     4381 ttcgtgaaca gcgggggatt gccctcggtc ggattcggca gcagcttggc gaaaatatcg
     4441 agcagctcgc cgacgcctgc gccatttttg gctgacacaa agcacaccgg aacgagatgc
     4501 ccctcgcgca gagctttctc gaatggcgca tgcagctgct cgggcgcaat ctccccctgg
     4561 tccaagtaga gcgacatcag ctcctcgtcg acctccacaa cctggtcgac cagcgcgcgg
     4621 tgcgcatcgg cgaccgacga aaaatcggct tcgccggcag gattgaaaaa acaatcgacg
     4681 acgcgcttcc cgttgccggc cgggaggttt atcggcaggc actctttgcc gaatgtcgcg
     4741 cggatcgcgg cgaggatcgc cggcaagtcg acgttctctg catcgatctt gttgacgatc
     4801 agcaggcggc aaagtctgcg cgccgccgcc cattccatca ttcgcgacgc gatcatttcg
     4861 atgcccgccg ccgcgttgat gacgatcgcc gcggtctcga cggcgtcgag ggcgccgatc
     4921 gattggccga tgaagtcggg aaatccgggc gtgtcgatga tgtgcacgcg cgtgtcctgt
     4981 gtctcgaggt gaagcaccga ggaccgcagc gagtgctgcc agcttctttc gagcgcctcg
     5041 aagtcactga cggtcgtgcc gcgctcgacg ctgccgaccg actggatcgc tcccgccttg
     5101 tgcagcagcg cttcgaccag cgtggtcttg cccgatgcgc cgtgcccgac cagcgccagc
     5161 gtgcgaatgt tgggggtggc gaatttcgcc atgaatgtca tctcctcctg ggaacctgcg
     5221 ggatggccgt gctcgttgcc gcgacggcaa ttctaccttg cagcgccggg acggcgctgg
     5281 cggctttgcc gcggcgccat tccaatttac gcccgctgcc ggccgaggtc aaagcgtcgt
     5341 ttgccgcccg caagcagcga gggcgcatcg tcgacgcgcc ctcgcttgac tccggcccga
     5401 ctgcagatca gttcgcgtgc acggcgagca gctcgcgcag attgcgaagc aactcttctt
     5461 cctggtaggg cttgccgaga tacaggtcga tgccgagctc ggcggcccgg ttgcgatgct
     5521 tttccgccgt ccgcgaggtg atcatgatga tcgggatgtc caccaggttg tggtcggcct
     5581 tgatcgtctt ggcgaattcg aagccgtcca tgcgcggcat ctcgatatcg agcaggatgg
     5641 catcgggtgt gcgctcgccc agcagctgca aggcgtcgag accgtccttg gccgcgatca
     5701 ccatgtagcc ttcgcgggtg agcagtcggc tggttatctt gcgcaccgtc agcgagtcgt
     5761 cgacgatcat gatcagcggc ggacccgctg cggcggcagc ggccgcggca gcggccgcgg
     5821 cttgcggcgt cttgacgacc atcggcgctt cggcggcggt gtccagtcgc tgcgccagct
     5881 gcaccgggtt gatgatcagc acgatctcgc cgttgccgag cacggtggcg ccggagatgc
     5941 cggacacgcg cgcgagctgc gggccgatgt tcttcacgac gacttcctgg ttgccgatca
     6001 tctcgtcgac gtgcaccgcg gccacgccct gcccggcgcg cagcagcagc accgagttgt
     6061 ggcgcgccgt ttccggcgcg tgcgcggcgt cgccgagcaa ccgcggcaga tagtggaac
//
LOCUS       DATANS010000429         6867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_695602, whole genome shotgun sequence.
ACCESSION   DATANS010000429 DATANS010000000
VERSION     DATANS010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6867
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695602"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VHT22_06270"
     CDS             complement(<1..259)
                     /locus_tag="VHT22_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019864404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137193.1"
                     /translation="MSVRKDLADYGEATRKPIDRGAAASRPDKVGFVIAGTQKGGTTA
                     LASYLYEHPEICVAGAKEVHFFDTDSLFRAGTVPDYAAYHAR"
     gene            complement(265..1089)
                     /locus_tag="VHT22_06275"
     CDS             complement(265..1089)
                     /locus_tag="VHT22_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019864404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HEX3137194.1"
                     /translation="MAGRNEPCPCGSGKRYKDCHGSLAAQARVGFVIAGTQKGGTSAL
                     ASYLFEHPEIAMPTVKEVHFFDNEDNFQSAPVDYARYHAYFKPAERRKVLCDATPIYM
                     YWDAAPPRIWQYNPAMKLVLLLRNPITRAYSQWNMERERGSDPLPFEQAIRTEAERCR
                     EALPLQHRLYSYVDRGFYSDQLRRIWRYFPVEQTLVLKSEELQHAPEAALARITDFLG
                     IARFAAVAPRSVHARPYQGPMSAEARRYLCDVFEFEIRNLERTFGWNCAKWLEAGA"
     gene            complement(1206..2828)
                     /locus_tag="VHT22_06280"
     CDS             complement(1206..2828)
                     /locus_tag="VHT22_06280"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX3137195.1"
                     /translation="MEAFARLYAELDATTSTHAKLDALKRYFAVAAPADAAWAAYFLA
                     GGRPRQAVPARVLRQMAIERAGIDDWLFEECYQAVGDLAETIAHVLPPPRTKSSAGLA
                     EWIEQRLLPLRGMPPDEIGERLAGYWDELDWSGRFLLTKLIGGGFRVGVSRLSVIRAL
                     AEVAGVEPKAVAQRIVGYTGIAAIPSSASFSALIASGPDGVSDAGQPYPFFLAHQLND
                     APDTLGPVDQWMVEWKWDGIRGQLVKRAGAIWLWSRGEELVTERFPEFHALQARIPDG
                     TVIDGEIVVWKDGRVRPFAALQTRIGRKSLPAKILAEAPAAMLAYDLLEENGADLRML
                     PQHERRARLQLLVPSIGDEHLQISPLVSLPTWDAYARLRAESRSRGVEGFMLKRRDAR
                     YGVGRTKESGTWWKWKIDPMTIDAVLIYAQRGHGRRASLYTDYTFAVWSEEAESGRRL
                     IPFAKAYSGLSDEEIKEVDAIIRKTTIEKFGPVRSVTPTLVFELGFEGIARSPRHKSG
                     IAVRFPRILRRRIDKPVDEADTLDNLNALLRS"
     gene            complement(2878..3933)
                     /locus_tag="VHT22_06285"
     CDS             complement(2878..3933)
                     /locus_tag="VHT22_06285"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HEX3137196.1"
                     /translation="MDLVVVQPEGLYCPQGDFYIDPWRPVARAVITHAHGDHARPGHA
                     HYLAAQASERVLKARLGEITLQTLSYGDSVVQNDVTVSLHPAGHVLGSAQVRLEHRGE
                     VWVISGDYKLEAEATCAPFEPLRCHTFVSESTFGLPIYRWRPQAEILLDIAAWWRRNA
                     QAQRASVLFCYAFGKAQRILAGLRAADALDIGKLICHGAIEPLNRAYRESGVDLPPTH
                     NVGDVSKADISSALILAPPSAGGTPWMRRFGNFSDGFASGWMQLRGARRRRSLDRGFV
                     LSDHADWPGLNAAIAATGASRVIITHGQIAVMVRWLQQQGFDARAFATEFEDDGGDEE
                     AQPPEAAAQAEAVESAE"
     gene            complement(3961..4911)
                     /locus_tag="VHT22_06290"
     CDS             complement(3961..4911)
                     /locus_tag="VHT22_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX3137197.1"
                     /translation="MSGQAAAARRRPGTNPLEILRHPGSFVTCVLKGFRANQGLLLAG
                     AVAYYTLLSMIPLLILMLIALSNFIDEARLFATLSEYLQFVVPGQADALVNELRHFLR
                     NRSEVGGVLLVTMILFSALAFTVLENAMSVIFIHRVAVRRRRFIVSALLPYLFMFFLA
                     IGMVVVTVVAGKLAVLATRDITVFGVPHSLGDLSGYLLYLLGVVGEILMLTAIYLVMP
                     VGRLSFRHAFVGGISAGLLWEITRHILVWYYGTISQVRLLYGSLATAITVMLSVEIAA
                     IVLLLGAQVIAEYERIGRERAETPPKPMDLKEPSSGTPAG"
     gene            complement(4908..5468)
                     /locus_tag="VHT22_06295"
     CDS             complement(4908..5468)
                     /locus_tag="VHT22_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017444968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX3137198.1"
                     /translation="MRVAILATDGFEQSELLEPRKALEAAGAATVLIAPKAGSIAGWR
                     HGAWGQSVPVDLTLDKTQADDYDALMLPGGVMNPDRLRTNKAAVAFVRAFFDSEKPVA
                     AICHAPIMLIEADVLHDRKLTSWPSLQTDIRNAGGQWIDEEVVVDRWLVTSRKPEDIP
                     AFNRQMIETIAEGAPSARMDRGSVEA"
     gene            complement(5526..6494)
                     /gene="egtD"
                     /locus_tag="VHT22_06300"
     CDS             complement(5526..6494)
                     /gene="egtD"
                     /locus_tag="VHT22_06300"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX3137199.1"
                     /translation="MALTALLPQAERAFAADVRAGLMRSPQKELSSRYLYDTIGSGLF
                     EVITALPEYGVTRAEERIVRSHAAEMVSRLPAHGLVAELGSGSGRKTRWLLEAIGRRH
                     PVTYCPVDISPSALALCRHELADIPGVRVVGYERDYLDGLRQIVSERKGAMPLLVLFL
                     GSTIGNFRRLAATRFMREVRALLQPGDAFLLGVDLIKPEHQLLPAYDDALGVTAAFNL
                     NLLARINRELGGQFDLRRFAHEARFDADTRSVDMHLRSLDDQQVSIPGADLVIRFERN
                     ETIWTESSHKYLAEEVAAMGEIAGFRCDGQWIDRIWPFADTLFGIA"
     gene            complement(6598..>6867)
                     /locus_tag="VHT22_06305"
     CDS             complement(6598..>6867)
                     /locus_tag="VHT22_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEX3137200.1"
                     /translation="DVAGIEGVRRALSDWLDWMGLIDAGPKARLDRFIGYYEPYATSH
                     EALDRDEAVQDEVRNVAQALVEAVGELRAGRLSAPGADLAAPRPK"
BASE COUNT         1201 a         2291 c         2214 g         1161 t
ORIGIN      
        1 aacgggcgtg ataggcggca tagtcaggca cggtacctgc gcgaaacagc gagtcggtgt
       61 cgaaaaaatg gacctccttg gcgccggcaa cgcagatctc ggggtgctcg tacagatacg
      121 aagcaagcgc agtcgtgcct cctttctgcg tacctgcgat gacaaaaccg accttgtcag
      181 gcctggatgc tgcggcgccg cgatcgatcg gcttcctcgt ggcttcccca tagtcggcga
      241 ggtccttgcg cacgctcatc ggctttaagc tcctgcctca agccatttgg cgcaattcca
      301 tccgaatgtg cgctcgagat tcctgatctc gaactcgaag acatcgcaga ggtagcgccg
      361 cgcctcggcg ctcatcggcc cctgataagg ccgcgcatgc acgctgcgcg gcgcgaccgc
      421 ggcgaaccgg gcgatgccga gaaaatcggt gatgcgcgcc agcgcagcct cgggcgcatg
      481 ctgcaactcc tcgcttttga gcaccagcgt ttgctcgacc gggaaatagc gccagatccg
      541 gcgcaactgg tcgctgtaga agccgcggtc gacgtacgag tagaggcgat gctgcagggg
      601 cagcgcctcg cggcagcgct cagcctcggt gcggatcgcc tgctcgaacg gcaacggatc
      661 gctgccgcgc tcgcgttcca tgttccactg cgaataggcg cgcgttatcg gattgcgcag
      721 cagcaggacg agcttcatcg ccggattgta ttgccagatg cgcggcggcg cggcatccca
      781 gtacatgtag atcggcgtcg cgtcgcagag aacttttctg cgttccgccg gcttgaaata
      841 ggcgtgatag cgagcgtagt cgaccggcgc ggattggaag ttgtcctcgt tgtcgaagaa
      901 atgcacttcc ttgacggtcg gcatggcgat ctcggggtgc tcgaacagat acgacgcgag
      961 cgcggacgtg ccgccttttt gcgttcccgc gatgacgaat ccaaccctgg cttgcgccgc
     1021 caggctgccg tggcaatcct tgtagcgctt gccgctgccg cagggacatg gctcgttgcg
     1081 acccgccacc gtcgatacct cccgtcgttc cggccgataa cgtcagccgc caaggcgtgc
     1141 gctcggccag ggccggcgcc gcgaaaaagc cgttactata ccttcgcttg gcgcacgggc
     1201 gaccgctacg accgcagcaa cgcattgaga ttgtcgagcg tgtcggcctc gtcgacgggc
     1261 ttgtcgatgc gccggcgcag gattcgcgga aagcgcaccg cgatgcccga cttgtggcgc
     1321 ggcgaccgcg cgatgccttc gaaaccgagc tcgaacacca gtgtcggcgt gacgctgcgc
     1381 accgggccga atttctcgat cgtcgtcttg cggatgatcg cgtcgacctc tttgatctct
     1441 tcgtcgctca agcccgagta cgccttggcg aacggtatca gccgtctgcc gctctctgcc
     1501 tcttcggacc agaccgcgaa agtgtaatcg gtatagaggc tcgcgcggcg cccgtgcccg
     1561 cgctgcgcat agatcagcac ggcgtcgatc gtcatcgggt cgatcttcca tttccaccag
     1621 gttccggatt ctttggtccg gccgacgccg taccgcgcgt cgcgccgctt gagcatgaat
     1681 ccttcgacgc cgcgcgatcg cgactcggcg cgcagtcggg cataggcgtc ccacgtcggc
     1741 aacgacacca gcggcgagat ctgcagatgc tcgtcgccga tggacggcac gagaagctgg
     1801 agccgcgcgc ggcgctcgtg ctgcggcagc atgcgcaaat cggcgccgtt ctcttccagc
     1861 aaatcgtagg cgagcatcgc ggccggcgcc tcggccagga ttttcgccgg cagcgatttc
     1921 cttccgatgc gggtctgcag tgcggcaaac ggtcgaacgc ggccatcctt ccacacgacg
     1981 atctcgccgt cgatgactgt cccgtcggga atccgcgcct ggagcgcgtg gaattccgga
     2041 aagcgctcgg tcaccagctc ctcgccgcgc gaccagagcc agatggcgcc tgcgcgcttg
     2101 accagctgtc cgcggatgcc atcccatttc cattcgacca tccattggtc gaccggcccg
     2161 agcgtatccg gcgcatcgtt cagctggtgc gccagaaaaa aagggtacgg ctggccggca
     2221 tcggagacgc catccggtcc ggaagcgatc agcgcggaaa aacttgccga gctggggatc
     2281 gccgcgatgc cggtatagcc gacgatgcgc tgcgcgacgg ccttcggctc gacgccagcg
     2341 acttcggcga gggcgcggat caccgaaagc cgcgacacgc cgacgcggaa tccaccgccg
     2401 atcagcttgg tcagcaggaa tcgccccgac cagtcgagct cgtcccagta gccggcgagg
     2461 cgctcgccga tttcgtccgg tggcatgccg cgcagaggca gcaatcgctg ctcgatccat
     2521 tcggccagcc cggcgctgct cttggttcgc ggtggcggca gcacatgcgc gatcgtctcg
     2581 gccaggtcgc cgaccgcctg atagcattcc tcgaacagcc agtcgtcgat gccggcacgc
     2641 tcgatggcca tctggcggag cacccgcgcg ggcaccgcct gccgcgggcg gcctccggcc
     2701 agaaaatagg cggcccatgc cgcatcggcg ggtgctgcaa cggcgaaata gcgcttgagt
     2761 gcgtcgagct tggcatgggt cgacgtcgtc gcgtcgagct cggcgtagag tcgggcgaag
     2821 gcttccatca ggcgacccgg tcgctgtcgc gtctcgcgcg ccggaacgtc gcgccgctca
     2881 ttcggccgat tcgaccgcct cggcctgcgc ggctgcttcc gggggctgtg cctcctcgtc
     2941 gccgccgtca tcttcgaatt cggtggcaaa ggcacgcgca tcgaagccct gctgctgcag
     3001 ccagcgcacc atcaccgcga tctggccgtg cgtgatgatg acgcgcgatg cgccggttgc
     3061 cgcgatcgcg gcgttgaggc caggccaatc ggcgtggtcg gacagcacga agccgcggtc
     3121 gagcgaacgg cgacgccgcg cgccgcgcag ctgcatccag ccggacgcga aaccgtcgct
     3181 gaagttaccg aagcgccgca tccatggcgt gccgccggcc gaaggcggcg ccaggatcag
     3241 cgcgctggaa atgtctgcct tggagacgtc gccgacgtta tgcgtcggcg gcaggtcgac
     3301 gccgctctcg cggtaggcac ggttcagcgg ctcgatggcg ccgtggcaga tcagcttgcc
     3361 gatatcgagc gcatcggccg cgcgaagccc ggcaagaatc cgttgcgcct tgccgaacgc
     3421 atagcagaac agcacgctgg cgcgctgcgc ctgggcgttg cgccgccacc aggcggcgat
     3481 gtcgagcaga atctccgcct gcggccgcca gcggtagatc ggcaggccga acgtcgattc
     3541 gctgacgaac gtatggcagc gcagcggctc gaacggcgcg cacgtcgctt ccgcttcaag
     3601 cttgtaatcg cccgatatga cccagacttc gccgcgatgc tcgagccgca cctgcgccga
     3661 tcccagcaca tgcccggccg gatggagcga gaccgtcacg tcgttttgca cgacgctgtc
     3721 gccgtaggac agcgtctgca gcgtgatctc tcccagccgc gccttgagca cacgctccga
     3781 ggcttgcgcg gcgagatagt gagcgtgacc ggggcgcgca tggtcgccgt gcgcgtgggt
     3841 gattaccgcg cgcgccaccg gacgccacgg atcgatgtag aaatcgccct gcgggcagta
     3901 gagaccctcc ggctgaacga caaccaggtc catcgcgccc gccgtttcgc gacgccggcg
     3961 tcagccggca ggcgtcccgc tcgacggctc cttcaggtcc atcggcttcg gaggcgtctc
     4021 ggcccgctcc cgtccgattc gctcgtattc ggcgatcacc tgcgcgccca ggagcagcac
     4081 gatcgcggcg atctcgacgc tgagcatcac cgtgatcgcg gtcgctaacg atccgtacaa
     4141 caggcgcacc tgggatattg tcccgtaata ccagacgagg atgtgacgcg tgatctccca
     4201 gagcaatccc gcgctgatgc cgccgacgaa cgcgtgccgg aacgaaaggc ggccgaccgg
     4261 catcaccaga tagatcgccg tgagcatcag aatttcgccg acgacgccga gcaggtacaa
     4321 gagatatccc gacagatccc cgagcgaatg cggaaccccg aacaccgtga tgtcgcgggt
     4381 tgcgaggacg gccagcttgc ccgcaacgac ggtgacgacg accataccga tcgccagaaa
     4441 gaacatgaac agatacggca gcagtgccga cacgatgaat cgccggcgcc gcacggcgac
     4501 ccggtggatg aaaatcaccg acatcgcatt ttccagcacg gtgaaggcaa gcgcgctgaa
     4561 taggatcatc gtgaccaata gaacgccgcc gacttcactg cgattgcgaa gaaaatggcg
     4621 cagctcgtta accagcgcgt cggcctggcc gggaacgacg aactgcaggt attcggacag
     4681 cgtcgcgaac agccgggcct cgtcgatgaa attcgacagc gcgatcagca tgaggatcag
     4741 cagcggaatc atcgacagca gcgtgtaata cgcaaccgcc ccggccagca gaaggccctg
     4801 gttggcgcgg aaccccttga gcacgcacgt cacgaagctg ccggggtggc gcaagatttc
     4861 cagcgggttg gttcccggcc tccggcgcgc agccgcggct tgaccgctca tgcctcgacg
     4921 ctgccgcggt ccatgcgcgc gctcggcgcg ccctcggcga tcgtctcgat catttgccgg
     4981 ttgaacgccg ggatgtcctc gggcttgcgg ctggtgacca gccagcggtc gacgacgact
     5041 tcctcgtcga tccactgccc tccggcattg cgaatgtcgg tttgcagcga cggccacgag
     5101 gtcaacttgc gatcgtgcag cacatcggcc tcgatcagca tgatcggcgc atgacagatc
     5161 gcggcgaccg gtttttcgct gtcgaaaaac gcgcggacaa acgcgaccgc cgctttattg
     5221 gtccgcagcc gatccggatt cattacaccg cccggcagca tcagcgcgtc atagtcgtcg
     5281 gcctgggtct tgtcgagcgt caggtcgacc ggcacgctct gaccccatgc gccatggcgc
     5341 catccggcga tcgatcccgc cttcggggcg atcaagacgg tcgcggcccc ggccgcctcg
     5401 agcgctttgc gcggctcgag aagctcggac tgctcgaagc cgtcggtcgc gagaatcgcg
     5461 acccgcatgc ccttcagtgg gccagtgttc gcagtcgttg gcttagccat cgtcgcagct
     5521 ctcgatcacg cgatgccgaa caaggtgtcg gcaaacggcc agatgcgatc gatccattgt
     5581 ccgtcgcagc gaaatccggc gatttcgccc atcgccgcga cctcctcggc cagatacttg
     5641 tggctgctct cggtccagat ggtctcattg cgctcgaacc ggatcacgag gtccgcgccc
     5701 ggaatgctga cctgctggtc gtcgagcgat cggaggtgca tgtcgacact gcgcgtgtcg
     5761 gcatcgaagc gcgcttcgtg agcaaagcgg cgcaggtcga actgaccgcc aagctcgcgg
     5821 ttgatacgcg ccagcaagtt gagattgaac gccgcggtga cgcccagcgc atcgtcgtac
     5881 gctgggagca gctggtgttc cggcttgatc agatcgacgc cgagcagaaa tgcgtcgccc
     5941 ggttgcagca gtgcgcggac ttcgcgcatg aatcgcgtcg ccgcgagccg gcggaaattg
     6001 ccgatggtgc tgccgaggaa cagtaccagc agcggcatcg cgcccttgcg ctcggacacg
     6061 atctgccgca agccgtcgag ataatcgcgc tcatagccga cgacgcgcac gcccggaatg
     6121 tcggccagct cgtgccggca cagcgcaagc gcgctcggcg agatatcgac cgggcagtaa
     6181 gtcaccggat gtcgccggcc gatcgcttcg agcagccagc gcgtcttgcg tccgctgccg
     6241 ctgcccagct cggcgaccag cccgtgcgcc ggaaggcggg aaaccatctc ggccgcgtgg
     6301 ctgcgtacga tccgctcctc ggcgcgagtc accccgtatt cgggcagtgc cgtgatgacc
     6361 tcgaacagcc ccgaccctat cgtgtcatac agataccgcg aggaaagctc cttctgcggg
     6421 ctgcgcatca ggccggcacg aacgtcggcg gcgaaggcgc gttcggcttg cggaagcagt
     6481 gcggtcaggg ccatggtttc ctcgtgtgac tgcgttcaac gttcactgca tattgcgccg
     6541 gcggggccaa tcacaagacg gtgcgaatcg actgatccgg cgacggtcgg agctcttcta
     6601 tttcggtcgc ggggcagcga ggtcggcgcc cggcgcggaa agacgtcccg cgcgcaattc
     6661 gccgacggct tcgaccagcg cctgtgcgac attgcgcact tcgtcctgga ccgcttcgtc
     6721 gcggtccagc gcttcgtggc tggtcgcgta cggctcgtaa tagccgatga agcgatcgag
     6781 ccgcgctttc ggccccgcat cgatcaggcc catccagtcg agccaatcgg acagcgcgcg
     6841 tcgcacgccc tcgatgcccg cgacgtc
//
LOCUS       DATANS010000430         1624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_105871, whole genome shotgun sequence.
ACCESSION   DATANS010000430 DATANS010000000
VERSION     DATANS010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105871"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VHT22_06310"
     CDS             complement(<1..386)
                     /locus_tag="VHT22_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX3137201.1"
                     /translation="MADTAGLRERAEETALLWVLVAIVAILSLVPLARLLFEGVAPGG
                     RFSTAALSNVLSSATTWTAAQHSLVTACGGTALAVLIGATVAVLVALTDIRGRNAFVF
                     CFVLPLMIAPQVVALAWLQLFGPSSTL"
     gene            complement(410..1405)
                     /locus_tag="VHT22_06315"
     CDS             complement(410..1405)
                     /locus_tag="VHT22_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3137202.1"
                     /translation="MNYLLRGVLLAAALVSMLAPLSAQAQAPKGKLVLYTSQPERDAT
                     QTTAAFKQAYPGVEVEVFRSGTTEVMGKLAAEFAAGAPKADVLLIADAASMEALKKDG
                     RLLPYPEARVAGLAAGTYDADRTYFGSKLITTGIAVNTAAKSRPASWADLAKPENKGQ
                     IAMPSPLYSGAAAIMLGTMTARPDLGWAFFEKLKADEAVAVRGNGAVLTAVASGEKPY
                     GVLVDFMAFNAKAKGSPIEFIFPAEGVPAVTEPVAMLKTAQNPAAGRAFIDFILSDEG
                     QKLALSMGYIPAKASVGMPSWLPPGTVIKLMPTDIGNVVQNNSANLKRFSELFGN"
     gene            1426..>1624
                     /locus_tag="VHT22_06320"
     CDS             1426..>1624
                     /locus_tag="VHT22_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137203.1"
                     /translation="MPFDTGSVSSPVLIPSAVRHGNDCSAAGQACFHDHKDHDRPSGC
                     SIASRKSAMKRAAVTPSTIRWS"
BASE COUNT          283 a          558 c          515 g          268 t
ORIGIN      
        1 agcgtgctcg acggtccgaa caactgcagc cacgcgagtg ccacgacctg cggcgcgatc
       61 atcagcggca gcacgaagca gaagacaaag gcgttgcggc cgcggatatc ggtcagtgcg
      121 accagcaccg caaccgttgc gccgatcagc accgccagcg ccgtgccgcc gcatgccgtc
      181 accaggctgt gctgggccgc ggtccaggtc gtggcgctcg acagaacgtt cgacagcgcg
      241 gcggtcgaga accggccacc gggtgcgacg ccctcgaaca gcaaccgggc cagcggcacc
      301 agcgacagga tcgcgacgat cgcgaccagc acccacaata gcgcggtctc ctcggcgcgc
      361 tcgcgcaagc ctgcggtgtc cgccatcgtg gcgcgccggc ggatcggggt cagttaccga
      421 agagctcgga aaaacgcttc agattggcgc tgttgttctg caccacgttg ccgatgtcgg
      481 tcggcatcag cttgatgacc gttccgggcg gcagccacga cggcatgcct acgctcgcct
      541 tggccgggat gtagcccatc gacagcgcga gcttctgccc ttcgtccgac aggatgaagt
      601 cgatgaatgc gcgcccggca gcgggattct gcgctgtctt caacatcgcg accggctcgg
      661 tcaccgcggg cacgccttca gcggggaaaa tgaactcgat cggcgagccc ttcgccttgg
      721 cgttgaaggc catgaagtcg accagcacgc cgtagggctt ttccccactc gcgacggcag
      781 tcagcaccgc gccgttgccg cgaaccgcca ccgcctcgtc agccttgagc ttttcgaaaa
      841 aagcccagcc gagatccgga cgcgcggtca tcgtgccgag catgattgcc gcggcgcccg
      901 aatacagcgg gctcggcatc gcgatctggc ccttgttttc cggcttggcg agatcggccc
      961 aggaagccgg acggctcttg gccgcggtgt tgaccgcgat gcccgtcgtg atcagcttgg
     1021 agccgaaata agtgcgatcg gcgtcgtaag tgcccgccgc gagcccggcg acccgggctt
     1081 ccggatacgg tagcagccgg ccgtcttttt tcagcgcttc catgctagcc gcgtcggcga
     1141 tcagcagcac atcggccttc ggcgctccgg cggcgaactc ggcggcaagc ttgcccatca
     1201 cctcggtcgt gccggagcga aatacctcga cctccacgcc gggatacgcc tgcttgaacg
     1261 ccgcagtcgt ctgcgttgcg tcgcgctcgg gttgcgacgt gtacagcacg agcttgccct
     1321 tcggggcctg cgcttgcgcc gacaggggcg ccagcattga gaccagcgcc gcggcgagca
     1381 atacgccgcg caaaaggtaa ttcatatcgc tcctccagga tgaaaatgcc gttcgacacc
     1441 ggatcggtgt cgtcgcccgt tcttatacct tccgccgtcc gccacggcaa cgactgttcc
     1501 gccgccgggc aggcatgctt ccacgaccac aaagatcacg acaggccttc cggatgctcg
     1561 atcgcctcgc gcaagtcggc gatgaagcgc gccgcggtta cgccgtcgac gattcgatgg
     1621 tccg
//
LOCUS       DATANS010000431         3332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_695708, whole genome shotgun sequence.
ACCESSION   DATANS010000431 DATANS010000000
VERSION     DATANS010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695708"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1511
                     /locus_tag="VHT22_06325"
     CDS             <1..1511
                     /locus_tag="VHT22_06325"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137204.1"
                     /translation="AGEWFNLGLVLAERNDPVGARNAYGRARALDPADLRALFGEQLT
                     LPMVYADAKEVDDARAAFEAGLDRLEGELLPAIRAAGLDETQALDGLRWVNFFLAYQG
                     RDDRDLQRRYAAVAAAAVDAVAPRWRAPLAGRSLRGKRVRVGFASAFFHTGTCGRYFK
                     SWITDLDRERFEVFVYHLWPGMDDIARAIATRADHFRTFAANRSRPSVVAPAIRGDEL
                     DVLVYTELGMDVTTFGLAALRLAPRQYTAWGHPVTTGHATIDAFISCAAMESAEAQQH
                     YSEPLVLLPGIGTRYERPVLPTPGSREQYSLPADRALLLCPQSLWKIHPDNDRLFAQI
                     LAANSRAMLVFFGGWHPAPIDRFMQRLKTTLDAHGIPIRERTRVLPQVGHDDYLRINM
                     LCDAMIDTLHWSGGNSSLDALACSLPVVTLPGAFMRGRQSAGMLSLLGVPELIAADHA
                     GYLAIAARLAGDESWRQELAARIGAAQSRLFDVSDAIERLQLLLQADDIRSV"
     gene            complement(1503..>3332)
                     /locus_tag="VHT22_06330"
     CDS             complement(1503..>3332)
                     /locus_tag="VHT22_06330"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3137205.1"
                     /translation="DLAVGHYRRALLRAPGHAGVLNNLGLTYEAQGRSAEAEACYRVV
                     LAATPNHPDALANLANIQFGREDFAAAAESYTRAFAIRRDAPAWIWVKRGIAQDRVHD
                     MAGAEASFLEAARLAPDDLQIHDNLATHQMGLGHYAEAEPTLQRMLALDPGNPYALSM
                     LAHARQHRCIWTGLDDLFAQVRAALAAPPHGDGRYTVNPFPLLAMPCDARAQLRAAQR
                     WAATKAPPAPAARPRPTMPAGARLRVGFVSSDLREHPMMHLQLEHWERIDRARLETFG
                     YGLRATDPGPIGRRAARAFEHFADISAESNEQILRRIRADGIAILFDLNGYTAGGDSR
                     LMGLRPAPIQINSIGFPGTLGVDWYDYILVDRFGAPEAMQPFYTERLWHMPHASYPSD
                     TTRAPLGPPPSRAECGLPERGFVFCCFNKSAKILPAVFAIWMRLMHAVPASVLWLLAS
                     EAEATANLQREAAAAGVDPARLIFAPKVPQQQHLARHVAADLFVDTFPYGAHTTTNDA
                     LLAGLPVLTCAGETLVTRLAGSQLHAIGLPELVTGDLTAYETLALRLAREPQTLAGFR
                     TRLAANRATHPLFDMARYTRDFEDGLQAIWRDFLAEQTRTPSD"
BASE COUNT          520 a         1077 c         1179 g          556 t
ORIGIN      
        1 gcgccggcga gtggttcaac ctcgggttgg tcctggccga gcgcaacgat ccggtcggtg
       61 cgcgcaacgc ctatggccgc gcgcgtgcgc tcgatccggc cgacctccgc gcgctgttcg
      121 gcgagcagct gaccttgccg atggtttacg ccgatgcgaa agaggtcgac gacgcgcgcg
      181 cggcattcga ggccggactc gaccgcctcg agggcgagct cctgccggcg atacgtgccg
      241 cggggcttga cgagacacag gctctcgacg ggctgcgctg ggtcaatttc ttcctcgctt
      301 atcaggggcg cgacgatcgg gatctgcagc gacgttacgc cgcggttgcg gccgcggccg
      361 tcgatgccgt cgcgccgcgc tggcgggctc cgctggccgg acggagtttg cgcggcaagc
      421 gcgtgcgagt cggattcgcc tcggcgttct tccatacggg aacctgcggg cgctatttca
      481 agagctggat caccgatctc gatcgcgagc gcttcgaagt cttcgtctat cacctgtggc
      541 ctggcatgga cgacattgcg cgggcgatcg cgacgcgggc cgaccacttc cgcacgtttg
      601 ccgccaaccg ctcgagacct tcggtggtcg cacccgcgat ccgcggcgac gagctcgatg
      661 tgctggtcta caccgagctg gggatggacg tcaccacctt cggacttgcg gcactgcggc
      721 tggcgccgcg gcaatacacc gcgtggggcc atccggtcac gaccggacac gcgaccatcg
      781 acgcattcat aagctgtgcg gcgatggaaa gtgcggaggc gcagcagcac tactccgaac
      841 cgctggtctt gctcccgggg atcgggacac gttacgagcg gccggtcctg ccaacgcctg
      901 gcagccgcga gcaatattcg ctgcctgccg accgggcgct gctgctgtgt ccgcagtcgc
      961 tatggaagat ccacccggac aacgaccgct tgttcgcgca gatactcgcc gctaattcgc
     1021 gtgcgatgct ggtctttttc ggcggatggc atccggcgcc tatcgaccgc ttcatgcagc
     1081 ggctcaagac gacgctcgat gcgcacggca tcccgatccg cgagcggacg cgggtcttgc
     1141 cgcaggtcgg gcacgacgac tacctgcgca tcaacatgct gtgtgacgcc atgatcgaca
     1201 ccttgcactg gtcggggggc aacagcagtc tcgatgcgct ggcctgcagc ctgccggtcg
     1261 tcacgctgcc cggtgcgttc atgcgcggcc gccagagcgc gggcatgctg tccttgctcg
     1321 gcgtgccgga gctgatcgcc gccgaccacg ccggctatct ggcgatcgcc gcgcgcctcg
     1381 ccggagacga atcctggcgg caggagctgg cggcgcgcat cggtgcggcg caatcgcgcc
     1441 tgttcgacgt gtccgacgcg atcgagcggc tgcagctgct gctgcaggcc gacgatattc
     1501 gttcagtctg acggcgttcg agtctgctca gccaggaagt cgcgccagat cgcctgaagc
     1561 ccgtcttcga agtcccgcgt atagcgcgcc atgtcgaaca acgggtgcgt agcacggttc
     1621 gccgcgaggc gcgtgcgaaa tccagccagg gtttgaggct cgcgggccag gcgcagcgcc
     1681 agcgtttcgt atgcggtcaa gtcgccggtc accagctccg gcagcccgat cgcgtgaagt
     1741 tggctgccgg cgagtcgggt caccagcgtt tcaccggcgc aggtgagaac aggaagccct
     1801 gccagcagcg catcgttggt cgtcgtatgc gcgccgtacg gaaacgtgtc gacaaaaaga
     1861 tcggcggcaa cgtgccgcgc cagatgctgc tgttgcggca ctttgggcgc gaagatcaga
     1921 cgcgccggat cgacgccagc cgccgcggct tcccgctgca agttggcggt cgcctcggcc
     1981 tcgcttgcga gcagccacag aacgctcgcg ggcaccgcat gcatcaagcg catccagatc
     2041 gcgaacacgg caggcagtat cttggccgat ttgttgaagc agcagaacac gaagccccgc
     2101 tcgggcaaac cgcattcggc gcgcgatggc ggaggcccca gcggcgcgcg ggtggtatcg
     2161 ctgggatacg acgcgtgcgg catatgccag aggcgttcgg tatagaacgg ctgcatcgct
     2221 tcgggcgcgc cgaagcggtc gaccaggatg tagtcgtacc agtcgacgcc cagcgtgccg
     2281 ggaaatccga tcgaattgat ctggatcggc gccgggcgca atcccatcag gcggctgtcg
     2341 ccgccggcgg tatagccatt caagtcgaac aggatcgcaa tgccgtcggc gcggatgcga
     2401 cggaggattt gttcgttcga ctccgcgctg atgtcggcga agtgctcgaa ggcgcgggcg
     2461 gcgcgccggc ctatcggccc gggatcggtg gcccgcaaac cgtagccgaa ggtttcgagg
     2521 cgtgcgcgat cgatccgctc ccaatgctcg agctgcaggt gcatcatcgg atgctcgcgc
     2581 aggtcggacg agacgaagcc gacgcgcagc cgcgcgcctg ctggcatcgt cggcctgggg
     2641 cgcgctgccg gcgctggcgg ggccttggtt gcggcccagc gctgcgcggc gcgcagctgg
     2701 gcccgcgcgt cgcacggcat cgccagcagc ggaaacggat tgaccgtgta gcgtccgtcg
     2761 ccgtgcggcg gcgccgccaa tgcggcgcgg acttgcgcga acaagtcgtc gagtccggtc
     2821 cagatgcacc ggtgctggcg cgcatgagcg agcatcgaca gcgcatacgg attgcctggg
     2881 tcgagcgcga gcatccgctg caaggtcggc tcggcttcgg cgtagtgccc gagacccatc
     2941 tggtgggtcg cgaggttgtc gtggatctgg aggtcgtcgg gggcgagccg tgccgcttcc
     3001 aggaagctcg cctcggcgcc ggccatgtcg tggacgcggt cctgtgcgat gccgcgcttg
     3061 acccagatcc atgccggcgc gtcgcggcgt atggcaaacg cgcgcgtgta ggattcggca
     3121 gcggcggcga agtcttcgcg tccgaactgg atattggcga gattggccag cgcgtcggga
     3181 tgattggggg tggcagcgag cacgacgcgg tagcaggcct cggcttcagc gctacggcct
     3241 tgcgcttcgt aggtgagccc caggttgttg agcacgccgg catgaccggg agcacgcaac
     3301 agcgcgcgcc ggtaatggcc gacggcgagg tc
//
LOCUS       DATANS010000432         1977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_358372, whole genome shotgun sequence.
ACCESSION   DATANS010000432 DATANS010000000
VERSION     DATANS010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358372"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VHT22_06335"
     CDS             <1..507
                     /locus_tag="VHT22_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase"
                     /protein_id="HEX3137206.1"
                     /translation="IALPPRNAKFTNFPYRAYIHSPELAVRLQAMSDYGQWNTQQPAR
                     LSEFAILITARQWSSQWIWRDHYTLAIKGGLDPKVALDLAAGRRPAGMKEDEAILYDL
                     AMQIYRDKAVTDTMYDKAQIQFGERGILDLLGIMGYYDTVAMILIAARAIPPHDDVTP
                     QLKPLATK"
     gene            796..984
                     /locus_tag="VHT22_06340"
     CDS             796..984
                     /locus_tag="VHT22_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137207.1"
                     /translation="MVVAGSLLAGCVVEDSRYGYRDGQRYSGDRDRDGVPNRADRDRD
                     GDGVRNSQDRRPDDPRRY"
     gene            1018..1491
                     /locus_tag="VHT22_06345"
     CDS             1018..1491
                     /locus_tag="VHT22_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137208.1"
                     /translation="MAEAADWQKLANALRELHRTLVEAARREYERAHLVELSPGELLQ
                     RLTSDAEFAWLRGLSELMVDLDLVHDAAPPDQADIAPAVRAAVEHLLSNAQTPAGVFA
                     QRYWPCVHDHPQIAMAHGAVRQALSAFPPAPPDTSTMLQERHRLAEKARHLAKRK"
     gene            1714..>1977
                     /locus_tag="VHT22_06350"
     CDS             1714..>1977
                     /locus_tag="VHT22_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137209.1"
                     /translation="MYDHIVALFRQHNIAFALAIFIAAAPAHARITRIVIDETISPAF
                     CKGAVCAAYGSAGQYEQLAGRAFGELDPRDPLNALIQDVALGKD"
BASE COUNT          385 a          632 c          604 g          356 t
ORIGIN      
        1 attgcgctgc cgccgcgcaa cgccaaattc accaactttc cgtatcgcgc gtatatccac
       61 agcccggaac tggcggtgcg cctgcaggcg atgtcggatt acggccagtg gaacacgcag
      121 cagcctgcgc gtctctccga gttcgcgatt ctgatcacgg ctcgccagtg gtcgtcgcaa
      181 tggatctggc gcgaccatta cacgctggcg attaaaggcg gcctcgatcc gaaagtcgcg
      241 ctcgatctcg cggcgggcag gcgtcccgcg ggcatgaagg aagatgaagc tattctgtac
      301 gaccttgcga tgcagatata ccgcgacaaa gcggtgacgg atacgatgta cgacaaggcg
      361 caaatccagt tcggcgagcg cggcattctc gatctgctcg gcatcatggg ctattacgat
      421 acggtggcga tgatcctgat tgcggcgcgg gcaattccac cgcacgacga cgttactccg
      481 cagttgaagc cgcttgcgac taagtagcgg gcattacgca cctgcggatt aagaatccgc
      541 gactcgctcg taatccctat acctacgccg cggcattttc gccgcggttt tttttcgcgc
      601 gtgcgctcaa tttgtggtgc aacgcacata cctgcggcgc gcagcggagt aaaaagaaag
      661 tcggagcgtg acgcaattcg tagcggcaat gcacgttgtt ctgccgcgca atccgccgtg
      721 agttgtgccg ggttcaccgt taagcgacta cgcatgattt ctttttttgc aatgaccgat
      781 taacaaggag atactatggt ggttgcaggc agcctgctgg ccggatgtgt cgtggaagat
      841 tcaagatacg gctatcgcga tggtcagcgt tacagtggtg accgcgaccg cgatggcgtt
      901 ccgaaccgcg cggaccgcga tcgcgacggc gatggcgtgc gcaacagcca ggaccgccgt
      961 ccggacgacc cgcgccgcta ctaatatttc aagcggcggc gcgaaaggag ctcgcgcatg
     1021 gcagaagcag cggactggca gaaactggcg aacgccctgc gtgagctgca tcgaacgctc
     1081 gtcgaggccg cgcgccgcga gtacgagcgc gcacatctcg tcgagctcag ccccggcgaa
     1141 ttgctgcagc ggctgaccag cgacgccgag ttcgcatggc tgcggggtct gtcggagctg
     1201 atggtcgatc tcgatctcgt gcacgacgcc gcgccgcccg atcaagcgga tatcgcgccc
     1261 gcggtgcgcg ccgcggtgga gcacttgctg tccaacgcgc aaacgcccgc cggcgtcttc
     1321 gcgcaacgct actggccgtg cgtgcacgat catccgcaga tcgcgatggc gcacggcgcc
     1381 gtcaggcagg cgctaagcgc atttccgccc gcgccgcccg acaccagcac tatgctgcag
     1441 gaaagacatc ggctcgccga aaaggcgcgc catctcgcga aacgaaaata gcccgtttgc
     1501 cgctctctgc cggctatgct acgttgcgca ctccagcagc gtcgcacgtt cgtaccggta
     1561 ataaacccgc agcgccccgc tagattcagg aaacgcctgc gtgccgcgtg acaacatgcg
     1621 atcgtgcgca cggtggtcga acgcctggac tcgccaacac gcacgtcgcg cgggcccatt
     1681 aagtaccatc agcacctcga gggaggccgc agcatgtacg accacatcgt cgcattgttt
     1741 cgccagcaca acatcgcgtt cgcgctcgcc atttttatcg ccgcggcacc ggcgcacgcg
     1801 cgcatcaccc ggatcgtgat cgacgaaacc atttctcccg cgttctgcaa aggcgccgtg
     1861 tgcgcggctt acggcagcgc cggccagtac gagcagctcg cgggccgcgc tttcggcgag
     1921 ctcgatccgc gcgatccgct caatgcactg atccaggacg tggcgctcgg caaggac
//
LOCUS       DATANS010000433         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_737544, whole genome shotgun sequence.
ACCESSION   DATANS010000433 DATANS010000000
VERSION     DATANS010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737544"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1592)
                     /locus_tag="VHT22_06355"
     CDS             complement(<1..>1592)
                     /locus_tag="VHT22_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEX3137210.1"
                     /translation="KDIDVAIPLNRLVCVTGVSGSGKSTLVQDVLYAALLKHKGKPTE
                     TPGAHRSIQGHHLIGDIVMVDQSSIGRTTRSNPASYVGAFDAIRELYAQLPVAKERGY
                     TRGTFSFNSGNGRCPTCGGNGFEHVEMQFLSDVYLRCPDCDGKRFRPEVLEVTLENKS
                     IADVLELTVFEALNFFSANPAVLTSLKPLADVGLDYLRLGQPVPTLSGGEAQRLKLAG
                     HLATAALPKRPAAAPRGKTGVKALLKSLFKPAPAQKTVAPPSNNTLFLFDEPTTGLHF
                     DDVAKLLRAFRQLIAAGNSVVVIEHNLDVIAASDWIIDLGPEGGEAGGAVVCTGMPAE
                     VRQHATSHTGKALREYEQALTTPIPTFPRKRGKESKSLLLNEGEGQGEGNSIRVHNAR
                     EHNLKNIDVAIPRNQFTVVTGVSGSGKSTLAFDIVFAEGQRRYLESLNAYARQFVQSA
                     ARPDVDAIFGIPPTVAIEQRTSRGGRKSTVATLTEIYHFLRLLYVKLGVQYCPDCDVP
                     IEPQTADSIVANLMRDYRNERI"
BASE COUNT          267 a          486 c          528 g          311 t
ORIGIN      
        1 cgatgcgctc gttgcggtaa tcgcgcatga ggttcgcgac gatgctgtcg gcggtctgcg
       61 gctcgatcgg cacgtcgcag tcggggcagt actgcacgcc cagcttcacg tacagcaggc
      121 gcaggaagtg atagatctcg gtgagcgtcg cgaccgtgct cttccgtccg ccgcggctcg
      181 ttcgctgctc gatggcgacg gtgggcggaa tgccgaaaat cgcgtcgacg tccggacgcg
      241 cggcggactg cacgaactgg cgcgcgtacg cattcagcga ttcgaggtag cggcgctggc
      301 cttcagcgaa cacgatgtcg aatgcgagcg tgctcttgcc gctgccggaa acgccggtaa
      361 cgaccgtgaa ttggttgcgc gggatcgcga cgtcgatgtt cttgaggttg tgttcgcggg
      421 cgttgtgaac tcttattgag ttgccctctc cctggccctc cccctcgttg aggaggaggg
      481 acttagactc cttcccccgt ttgcgaggga aggttgggat gggggtagtc agcgcctgtt
      541 cgtattcgcg caacgccttc cccgtatgcg aagtcgcatg ctgccgcacc tccgccggca
      601 tcccggtaca tacgacagcg cctcccgctt cgccgccttc aggacccaga tcgatgatcc
      661 agtcggaggc ggcgataacg tcgaggttat gctcgatcac gaccacggaa ttgccggccg
      721 cgatcaactg gcggaacgcg cgcaagagtt tcgcgacatc gtcaaagtgc aggcccgtcg
      781 tcggctcgtc gaacaggaac agcgtgttat tcgacggcgg agcgactgtt ttttgcgctg
      841 gagcgggctt gaacagcgat ttcaacagcg ctttcacgcc tgttttgccg cgcggcgccg
      901 cggccggccg cttcggaagc gctgctgtgg cgaggtggcc cgccagtttc agtcgctgcg
      961 cttcgcctcc cgacagagta ggcaccggct ggcccaggcg cagatagtcg aggccgacat
     1021 cggcgagcgg ctttaacgag gtcagcacgg ccgggttcgc tgagaagaag ttcagcgctt
     1081 cgaacaccgt cagctcgagc acgtcggcga tcgacttgtt ttcgagggtt acttcgagca
     1141 cttccgggcg gaagcgcttg ccgtcgcagt cgggacagcg caggtagacg tcggacagga
     1201 actgcatctc gacatgctcg aagccgttgc caccgcaggt cgggcagcgg ccgttgccgg
     1261 aattgaagct gaaggtgccg cgcgtgtagc cgcgttcttt ggcgaccggc aattgcgcgt
     1321 aaagctcgcg gatagcgtcg aacgcgccga cgtagctcgc cggattggag cgcgtcgtgc
     1381 gcccgatcga cgactgatcg accattacga tgtcgcctat caggtgatga ccctgaatcg
     1441 agcggtgcgc gcccggcgtc tcggtcggct tgcctttgtg cttcagcagc gccgcataca
     1501 gcacgtcctg gaccagcgtc gacttgcctg agcccgacac gccggtcacg cacacgagcc
     1561 gattgagcgg gatcgcgacg tcaatgtcct tg
//
LOCUS       DATANS010000434         1816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_737570, whole genome shotgun sequence.
ACCESSION   DATANS010000434 DATANS010000000
VERSION     DATANS010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737570"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VHT22_06360"
     CDS             <1..620
                     /locus_tag="VHT22_06360"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX3137211.1"
                     /translation="GDAIMAFWGAPVDNPNHARDAVLAALAMQRRMSSLAHEFKKRRW
                     PELVIGVGINTGAMNVGDMGSEFRKAYTVLGDAVNLASRLEGLTKEYGVAVIIGEDTR
                     AAVPDIRCREIDRVRVKGRANPITIFEPLPETEESTAGELAAWEKALLQYRERRFADA
                     AASLAALASAHPATRLYAYFAQRCATYAAARPPADWDGATNFARK"
     gene            complement(640..>1816)
                     /locus_tag="VHT22_06365"
     CDS             complement(640..>1816)
                     /locus_tag="VHT22_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137212.1"
                     /translation="GFRTLTGQIGKLNRDQYQVLTTQATIGIRGTHYQVQICAAGQCR
                     NRGAEVGSGMYGGAYEGGVVVANGFGSGEFGADEFFYVPDGQAPLRWLGPPDFLSDKL
                     ERRARVARTAPTELRFAKVPETAPPPALPQAPFTFMATEDLGSRLLAPGQTIVVGSDR
                     YTLELDSTSNPGLQVAVVDGALHSFHNGSLSADLGSAALTDVGSDGSIPSGFAAGSGL
                     NWGRWSGPGSTITQALGNQLVHNDGGDLHYIYGNTATALPTSGQVSYAAVGGTRPTDS
                     GTGAVGTLVSGGTVNVNFTTAQLGLSGLTAGFNDANYTMGGTASIINGRFSTGGVGGA
                     LTGCAGGACQPLVAGNFTGFFAGPGGSGIGIDYYFNIRTGNVIEGVVGYRKCGPGGC"
BASE COUNT          319 a          605 c          603 g          289 t
ORIGIN      
        1 tcggcgatgc gatcatggcc ttctggggcg cgccggtcga caatccgaac cacgcgcgcg
       61 acgccgttct cgcagcgctc gcgatgcagc gcagaatgtc gtctctggca cacgagttca
      121 agaagcgccg ctggccggag cttgtcatcg gcgtcggcat caacaccggc gcaatgaacg
      181 tcggtgacat gggctctgaa ttccgcaagg cttacacggt gctcggcgac gcggtcaacc
      241 tcgcatcgag gctcgaggga ttgaccaagg agtacggcgt cgccgtcatc atcggcgagg
      301 acacgcgcgc cgccgtgccc gacatccgct gtcgcgagat cgatcgagtc cgcgtcaagg
      361 ggagggcgaa cccgatcacg atattcgagc cactgccgga aacggaagag tcgaccgccg
      421 gtgaactcgc ggcgtgggag aaagctctgc tgcaataccg cgagcggcgc tttgccgacg
      481 ctgcggcgtc gcttgccgcc cttgccagcg cgcatcctgc tacgcgtctg tatgcctatt
      541 tcgcccagcg ctgcgcgacc tacgccgcgg cacgaccgcc ggcggattgg gacggcgcca
      601 ccaacttcgc gaggaaatag ccttcgcccg cctagtgcac tagcagccgc cggggccgca
      661 tttgcgataa ccgacgacgc cctcgatcac gttgccggtg cggatgttga aatagtagtc
      721 gatgcctatg ccggatccac cgggaccggc gaagaatccg gtgaagttcc cggcgaccag
      781 cggttgacaa gcgccgccgg cgcagcccgt caacgcaccg ccgacgccgc cggtcgagaa
      841 gcggccattg atgatgctgg ccgtgccgcc catcgtatag ttcgcatcgt tgaagccggc
      901 ggtgagaccg gagagcccga gttgagcggt ggtgaagttg acgttgaccg tgccgccgga
      961 caccagcgta ccgacggcgc cggtgcccga gtcggtcggc cgcgtgccgc cgaccgccgc
     1021 gtacgaaacc tgtccggatg tcggcagcgc cgtcgccgta ttgccgtaga tgtaatgcag
     1081 gtcgccgccg tcgttgtgaa ccagctgatt gccgagcgcc tgcgtgatcg tcgatccggg
     1141 ccctgaccag cggccccagt taagcccgct gccggcggca aagcctgacg gtatcgaccc
     1201 gtcgctgccg acgtcggtca gtgctgcgga gccgagatca gcggagagcg agccgttgtg
     1261 gaacgaatgc agcgcgccgt cgacgacagc gacctgcagg ccaggattcg acgtagagtc
     1321 gagctcaagc gtgtagcgat ccgacccgac gacgatcgtc tgccccggcg cgagcaagcg
     1381 cgagccgaga tcctccgttg ccatgaacgt gaacggcgcc tgcggcaacg ccggcggcgg
     1441 cgcggtttcg ggcaccttgg caaaacgcaa ttcggtcggc gcagtgcgcg cgacgcgcgc
     1501 gcgccgctcg agcttgtcgg aaagaaaatc aggcggcccg agccagcgca gcggcgcttg
     1561 tccgtcgggc acgtagaaaa attcgtcggc gccgaactcg ccgctgccga aaccgttggc
     1621 gacgacgaca ccgccttcgt aggccccgcc gtacattccc gaccccacct cggcgccgcg
     1681 gttcctgcac tggccggcgg cgcagatctg gacctgatag tgcgtaccgc gaatgcctat
     1741 cgtcgcctgc gtggtcaaca cctggtactg gtcgcggttc agtttgccga tctgtccggt
     1801 caacgtgcgg aatccg
//
LOCUS       DATANS010000435         1878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_653016, whole genome shotgun sequence.
ACCESSION   DATANS010000435 DATANS010000000
VERSION     DATANS010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653016"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..992)
                     /locus_tag="VHT22_06370"
     CDS             complement(36..992)
                     /locus_tag="VHT22_06370"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX3137213.1"
                     /translation="MHSRIAGTGGYLPAQIVTNADLAQRIDTDDEWVRTRTGICQRHI
                     AAPGEQTSDLALVAAQRALEAAKLAADDVDLIIVATTTPDMVFPSTACILQDKLGAKS
                     GPAFDVQAVCSGFVYALSIADRMVASGGAQNALVIGAEIYSRILDWDDRGTCVLFGDG
                     AGAVVLVPSERPGILAAHLHADGSYRDILSVPGTVAGGAVSGRPLLKMDGHAVFKFAV
                     KVLAETAQEALMSAGMAPGAVDWVIPHQANIRIMEATMKKLGLPLERMVSTVGLHANT
                     SAASIPLALDVAVRDGRVKKGSNVLLQGVGGGFTWGSVLLKY"
     gene            complement(985..>1878)
                     /gene="plsX"
                     /locus_tag="VHT22_06375"
     CDS             complement(985..>1878)
                     /gene="plsX"
                     /locus_tag="VHT22_06375"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX3137214.1"
                     /translation="PARDRLTLHAASEVVEMHEPPADALRRKKDSSMRVAINLVKDEV
                     AQACVSAGNTGALMAISRFVLKTLPGIDRPAIASQLPTRTGQTTVLDLGANVHCTPEQ
                     LVQFAVMGSALASAVYQIDRPTVGLLNIGEEDIKGNDVVKQTGELLKASDLNYYGNVE
                     GNDIYKGTVDVIVCDGFVGNVALKTSEGLALMLSDFLREEFTRNPLRKLLVVFALPAI
                     KAFKRRVDPRRYNGATLIGLKGIVVKSHGSADVFAFQQALKKAYAEAANGVLERIARR
                     LAAMPVPAQASAGGAGSIVDA"
BASE COUNT          316 a          641 c          597 g          324 t
ORIGIN      
        1 gccctggccc gggaagacga tcgcgagaga catggtcagt atttgaggag cacggagccc
       61 catgtgaagc cgccgccgac gccctgcagc agcacgttgg agcccttctt cacgcggccg
      121 tcgcgcaccg cgacgtcgag cgccagcgga attgatgccg ccgacgtgtt ggcgtgcagg
      181 ccgacggtcg agaccatgcg ctcgagcggc aggccgagct ttttcatcgt cgcttccatg
      241 atacggatat tcgcctgatg cggaatcacc caatcgacgg cgcccggagc catgccggct
      301 gacatcagcg cttcctgcgc tgtctcggcg agaaccttca ccgcgaactt gaacacggcg
      361 tgaccatcca tcttcagcag cggccggccg ctgaccgcac cgccggcaac cgtgcccggc
      421 accgacagga tgtcgcgata gctgccgtcc gcgtgcaaat gcgccgccag gatgcccggc
      481 cgttccgacg gcacgagcac gaccgcgccg gcgccgtcgc cgaacaacac gcaggtcccc
      541 cgatcgtccc aatcgagaat gcgcgaatag atttcggcgc cgatgaccag cgcattttgc
      601 gcgccgccgc tggccaccat gcgatcggcg atcgacagcg catagacgaa accgctgcag
      661 acggcctgga catcgaacgc cgggccgctt ttcgcgccca gcttgtcctg aagtatgcag
      721 gccgtcgacg ggaataccat atcgggcgtc gtcgtcgcga cgatgatcag atcgacatcg
      781 tcggccgcaa gtttcgccgc ctccagcgcg cgctgtgccg ccaccagcgc cagatcgctg
      841 gtctgctcgc ccggcgcagc gatatggcgc tggcagatgc cggtgcgcgt tcgcacccat
      901 tcgtcgtcgg tgtcgatccg ctgcgcaaga tcggcattgg tcacgatctg cgccggcaga
      961 taaccgccgg tcccggcgat gcggctatgc atcgacgatc gatccggcgc caccggcgga
     1021 agcttgcgcg ggaaccggca tcgccgccag ccggcgcgca atgcgctcga gcacgccgtt
     1081 tgccgcctcg gcgtacgctt ttttcagggc ctgctgaaac gcgaagacgt ccgccgagcc
     1141 atgacttttg acgacgatgc ccttgaggcc gatcaaggtc gcgccgttgt agcgccgggg
     1201 gtcgactcgc cgcttgaatg ccttgattgc cggcagtgcg aacaccacca ggagcttgcg
     1261 cagcggattg cgcgtgaatt cctcgcgcag gaaatccgaa agcatcagcg ccagcccttc
     1321 cgaggtcttc agcgcgacat tgccgacgaa gccatcgcag acgatgacgt cgaccgtccc
     1381 cttgtagatg tcattgcctt cgacattgcc gtaatagttg aggtccgagg ctttcagcag
     1441 ctccccggtc tgcttgacca cgtcgttgcc cttgatgtcc tcttcgccga tgttgagcag
     1501 gcccaccgtc ggacggtcga tctggtacac cgcgctcgct agcgcgctgc ccatgacggc
     1561 gaactgcacg agctgctcgg gcgtgcagtg cacgttcgcg ccgaggtcga gcaccgtcgt
     1621 ctggcccgtg cgcgtgggaa gctgcgaggc gatcgcggga cggtcgatgc ccggcagcgt
     1681 cttcagcacg aagcgcgaga tggccatgag cgcgccggta ttgccggccg acacgcacgc
     1741 ctgcgcgacc tcgtccttca cgagattgat cgcgacgcgc atcgacgagt ctttcttccg
     1801 gcgcagtgcg tcggccggcg gctcgtgcat ctcgacgact tcgcttgccg cgtgaagcgt
     1861 caggcggtcg cgcgcggg
//
LOCUS       DATANS010000436         1649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_231223, whole genome shotgun sequence.
ACCESSION   DATANS010000436 DATANS010000000
VERSION     DATANS010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231223"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..104)
                     /locus_tag="VHT22_06380"
     CDS             complement(<1..104)
                     /locus_tag="VHT22_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005319797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-[acyl-carrier protein] methyl ester
                     esterase"
                     /protein_id="HEX3137215.1"
                     /translation="MQLAVTGAGPDLVLLHGWGMRASVWVDLAALLAA"
     gene            complement(104..1261)
                     /gene="bioF"
                     /locus_tag="VHT22_06385"
     CDS             complement(104..1261)
                     /gene="bioF"
                     /locus_tag="VHT22_06385"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEX3137216.1"
                     /translation="MSFDLAADLAALETRGLRRVRRVLDSPQSARVVVDGRDYVAFCS
                     NDYLGLAADPRLIAAACNGARTDGIGAGASHLILGHTAAHHRLETALAQFVQLPRALS
                     FSTGYMANLGTVTALVGRHDDVFADKLNHASLNDAVQLSRANFRRYAHSDLDALERLL
                     AASCAPRKLVVTDAVFSMDGDIAPVPQSLQLCEQFDAWLLLDDAHGFGVLGAGGAGVL
                     SHFKVASPRVVYMATLGKAAGVAGAFVAGAPDLIETLIQNARTYIYTTATPPLLAHAL
                     LASLEIMRREDGRRETLGRLIEQLKTGLEGMRWKLMASPTAIQPLIVGGNAKVLRVSA
                     ALAERGVLVPAIRPPTVPDGTARLRISLSAAHATEDVARLVDALRHVEKNS"
     gene            complement(1343..>1649)
                     /locus_tag="VHT22_06390"
     CDS             complement(1343..>1649)
                     /locus_tag="VHT22_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX3137217.1"
                     /translation="VPINNLVQVEGTPLHGTAQLDPFEFVRTIAAARITMPKAMVRLS
                     AGRQEMSEGIQALCFAAGANSIFYGDKLLTTGNPEAQRDRALLDKLGMHPMKVPREA"
BASE COUNT          295 a          535 c          556 g          263 t
ORIGIN      
        1 ctcgctgcca gcagcgccgc gaggtcaacc catacgctgg cgcgcatgcc ccagccgtgc
       61 agcagcacga gatcgggacc ggcgccggtg accgcgagct gcatcaggaa tttttttcga
      121 cgtggcgcaa cgcgtccacg aggcgcgcca catcttccgt cgcgtgcgcg gccgacagcg
      181 aaatgcgcag gcgcgcggtg ccgtccggga cggtgggcgg ccggatcgcc gggaccagca
      241 cgccgcgctc ggccagcgcg gcagacacgc gcagaacttt ggcgttgccg ccgacgatca
      301 ggggttggat cgcggtcggc gaagccatca atttccagcg catgccctct aggcctgtct
      361 tcagttgctc gatcagtcga ccgagcgtct cccgccgccc gtcttcgcgc cgcataattt
      421 ccaggctcgc gagcagcgca tgggccagca gcggcggtgt cgccgtagtg taaatataag
      481 tgcgcgcatt ctggatcagc gtttcaatca gatccggcgc gccggcgacg aatgcgcccg
      541 ccacgccggc cgccttgccg agcgtcgcca tgtaaacaac gcgcggcgag gccaccttga
      601 aatgactcaa tacgccggcc ccgcccgcgc cgagcacgcc gaagccgtgc gcgtcgtcga
      661 gcaacagcca ggcatcgaac tgctcgcaca actgcaggct ctgcgggacc ggcgcgatgt
      721 cgccgtccat gctgaatacg gcatcggtca ccacgagctt gcggggcgcg cacgacgctg
      781 cgagcaagcg ctcgagcgcg tccaaatcgc tgtgcgcgta acgcctgaaa ttcgcgcgcg
      841 acaattgcac ggcatcgttc agcgacgcgt gattgagctt gtcggcgaac acgtcgtcat
      901 ggcggccgac cagcgccgtc accgtcccga ggttggccat gtaaccggtc gagaatgaca
      961 gcgcgcgcgg cagctgaaca aactgcgcga gcgcggtttc gagccggtga tgcgccgctg
     1021 tatggccgag tatcaaatgc gatgcgcccg cgcctatgcc atcggtgcgc gcgccattgc
     1081 acgcggcggc aatgaggcgc gggtcggcag cgaggccgag atagtcgttg ctgcagaaag
     1141 caacgtaatc gcggccgtcg accacgacac gggcgctttg ggggctgtcg agcacgcggc
     1201 gcacgcggcg caggccgcgc gtctcgagcg cggcgagatc ggcggcgaga tcgaaggaca
     1261 tgtgacgcag aaagcgctag tcaggcggcg ggcggtcagt gaaataaagg cgctgcggcg
     1321 gcgcgatttt acgcctcact gcttacgcct cacgcgggac tttcattgga tgcatgccaa
     1381 gcttgtcgag cagcgcgcga tcgcgttgag cttccggatt gccggtcgtc aagagcttgt
     1441 cgccgtagaa aatggagttt gcgcccgccg cgaaacaaag cgcctgaatg ccttcggaca
     1501 tttcctggcg gccggcggac aggcgcacca tcgctttggg catcgtgatg cgcgcggcgg
     1561 ctatcgtgcg cacgaattca aacggatcga gctgcgcagt cccgtgcagc ggcgtgcctt
     1621 cgacctggac caggttgttg atcggcacg
//
LOCUS       DATANS010000437         4048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_777847, whole genome shotgun sequence.
ACCESSION   DATANS010000437 DATANS010000000
VERSION     DATANS010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777847"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VHT22_06395"
     CDS             complement(<1..289)
                     /locus_tag="VHT22_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014692455.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX3137218.1"
                     /translation="MPESLRRVAVVGSARIPFARSNTRYIDAGNPEMLTAAFAALVER
                     FGLRGVQLGEVAAGAVIKHPRDWNLTREAALGAGLHPHTPAYDVQRACGTSL"
     gene            600..1325
                     /locus_tag="VHT22_06400"
     CDS             600..1325
                     /locus_tag="VHT22_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX3137219.1"
                     /translation="MMTMASFVETGSDLYAHNLVSYFDDDEVVAGWLAESWEHEEVQH
                     GAALRAYVEHVWPDFDWQRTYDRFFDEYRQTCTVPELEPARALELAARCVVEMGTATL
                     YSAMGAYADEPVLKDLAAHIYADEVRHYKHFYRYFRRYQERERHGRWRIGRTMTHRLL
                     ETAEDDGRIAFRHLWQATHRGSGSVEDEYRAFRDRISEVARGFAPRDLPVEMFLRPLR
                     LPPVAARGAKSCAGTLYRWWLEA"
     gene            complement(1353..1925)
                     /gene="folE"
                     /locus_tag="VHT22_06405"
     CDS             complement(1353..1925)
                     /gene="folE"
                     /locus_tag="VHT22_06405"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018624563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX3137220.1"
                     /translation="MKPNSKKIESLITELLVEIGEDPHREGLLKTPQRVAQAMEFLTS
                     GTRVDAQKLINGAVFTQATNSMVIVKNIEVYSLCEHHMLPFFGRCHIGYIPSGRVFGV
                     SKLARLVDMYARRLQLQERLTEQISQEVMDSVAAKGVGVMIEARHLCMMMRGVEKQNS
                     VMITSSVLGTFRESQATREEFLALIGNRAT"
     gene            2039..2707
                     /locus_tag="VHT22_06410"
     CDS             2039..2707
                     /locus_tag="VHT22_06410"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX3137221.1"
                     /translation="MGTMIELSAADGFRLAAYRADPEGPPRGGLVVVQEIFGVNSHIR
                     SVCDGYAADGYRAIAPALFDRYEKGIDLGYTAADIARGREYKARANSDAAMLDVDAAR
                     AAIAAAGKVGVVGYCWGGLITWLAACRLKGFACAISYYGGGILEAAAEQPKCPVIAHF
                     GERDHVIPVAGVRAFGEAHPDVGVYIYAADHGFNCDQRGSYDPAAATLARQRTLDFLR
                     QHVG"
     gene            2804..3763
                     /locus_tag="VHT22_06415"
     CDS             2804..3763
                     /locus_tag="VHT22_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HEX3137222.1"
                     /translation="MPTTAANKAVREARIAALRRFNRFYTQRIGVLQEGLLDSPFSLA
                     QVRVLYELAHPESYGDDGPTASELSARLGVDEGYLSRIVHGFESKGMLCRRPSTADAR
                     RQLLALTREGRAAFAPLDRQSHDEAGALLDTLSAGDQRRLVAATDAIESLLDRSAVPR
                     RAIALRAPRPGDIGWVVQRHGELYAREYGYDAQFEALVAGIAARFVQRFDAGHERCWI
                     ADCGGTNLGSVFVVRQSAHVAKLRLLLVDPAARGRGLGARLVAECIRFARRAGYRSMV
                     LWTQSELAAARHIYEQAGFRRTRRQAHHSFGRDLVGETWRLML"
BASE COUNT          654 a         1333 c         1375 g          686 t
ORIGIN      
        1 taagactggt gccgcaggcg cgctggacgt cgtaggcggg cgtgtgcggg tgcagaccgg
       61 cgccgagcgc ggcctcgcgc gtcaggttcc agtcgcgcgg atgcttgatc accgcgccgg
      121 ccgcgacttc gcccagctgt actcctcgaa gcccaaagcg ctcgaccagt gccgcgaagg
      181 ctgcggtcaa catttccggg ttgccggcat cgatataccg ggtgttgctg cgcgcaaacg
      241 gaatccgcgc gctgccgacg accgcgactc ggcgcaagct ttcgggcatc gctgatccgg
      301 ggcgtaccaa aacggccatt ctaggcgtgg gaccctcgga ataggacaca ggactattgc
      361 aactttcgcg ccgcggaggc atgttgtggt ctgcaaccgc gggattttct gcccgggacg
      421 ctgtcttaca aaaaagaccg acagggagtc atacgatgct tgatgcacga aggccggaac
      481 cgcgcgccgg gggcgcgcca ggccagggtt ggcgtgcgcg gggcgcctgg cgccttactg
      541 acatcgatta cgccggtgcc gaccgggcgc gggcggcgga tgaccatacg ctcttccaga
      601 tgatgaccat ggcctcgttc gtcgaaacgg ggtcggactt gtacgcgcac aatctggtgt
      661 cctatttcga tgacgacgaa gtcgttgccg ggtggctggc cgagtcgtgg gagcacgagg
      721 aagtgcagca tggcgcagcg cttcgcgcct atgtcgagca cgtctggccc gacttcgact
      781 ggcagcggac ctacgaccgc ttcttcgacg agtaccgcca gacctgcacg gtccccgagc
      841 tggagcccgc ccgggcgcta gagctcgccg cgcgctgtgt cgtcgaaatg ggcacggcca
      901 cgctctacag cgcgatgggc gcgtacgcgg acgagccggt actgaaggac ctggcggcgc
      961 atatctatgc cgacgaagtc cgccactaca agcatttcta ccgctatttc cgccgctatc
     1021 aggagcgtga gcgccacggg cgctggcgca tcggccgcac gatgacccat cggctgctgg
     1081 agacggcgga ggacgacggc cgcatcgcct tccggcatct gtggcaggca acgcaccgcg
     1141 gatccggctc ggtcgaagac gaataccgcg cgttccgcga ccggatctcg gaggtggcgc
     1201 ggggctttgc gccccgcgac cttccggtag agatgttcct gcggccgctt cgactgcccc
     1261 cggtcgccgc ccgtggcgcg aaaagctgcg ccgggacgct ctaccgctgg tggctggaag
     1321 cctgagcgcc gcttagcagc ggtatcgttc tctcaggtcg cgcgattgcc gatcagcgcg
     1381 agaaactcct cgcgcgttgc ctgtgactcg cggaacgtgc ccagcaccga cgaggtgatc
     1441 atcaccgaat tctgcttctc gacgccgcgc atcatcatgc acaggtgccg cgcctcgatc
     1501 atcacgccga cgcccttcgc ggcaaccgaa tccatcactt cctgcgagat ctgctcggtc
     1561 agccgttcct gaagctgcag ccggcgcgca tacatatcga ccaggcgcgc gagcttggac
     1621 acgccgaaga cgcgtccgct ggggatgtag ccgatgtggc agcggccgaa aaacggaagc
     1681 atgtggtgct cgcacaggct gtagacctcg atgttcttga cgatgaccat gctgttggtc
     1741 gcctgggtga acaccgcgcc gttgataagc ttctgcgcat cgacccgggt gccggaggtg
     1801 aggaactcca tcgcctgcgc aacccgttgc ggcgtcttga gcaggccttc ccggtgcgga
     1861 tcctcgccga tttcgaccag taactcggtg atcagcgact cgattttctt gctgttcggt
     1921 ttcattgccc ggtcccgttg aacaaacgcc tcattatccc acgcgggcat gcgtcgattc
     1981 cggcgtcgcg ccgcgatgcg ataatggccg gtcgaattga ccggtaaagg aacgcgcgat
     2041 gggaaccatg atcgaactct ctgccgccga cggattccgg ctggccgcct accgcgccga
     2101 cccggagggc ccgccgcgcg ggggcctggt cgtcgtccag gaaatcttcg gcgtcaacag
     2161 tcacattcgc agtgtctgcg acggttacgc cgccgacggc tatcgagcca tcgcgccggc
     2221 actgttcgac cgctatgaaa aaggcatcga cttgggctat accgcggcgg atattgctcg
     2281 cggacgcgaa tacaaggcac gcgcgaactc cgatgcggcg atgctcgacg tcgacgccgc
     2341 ccgcgccgcg atagcggccg ccggcaaagt cggcgtcgtc ggttattgct ggggcggcct
     2401 gatcacctgg ctggcggcgt gccggctgaa gggttttgcc tgcgcaatct cgtactacgg
     2461 cggcgggatc ctcgaagccg ctgccgagca gccgaaatgt ccggtgattg cgcacttcgg
     2521 cgagcgcgac cacgtcattc ccgtcgccgg cgttcgcgcc tttggcgaag cgcacccgga
     2581 tgtcggcgtc tatatctatg ccgcggacca tggcttcaat tgcgatcagc gcggctcgta
     2641 tgatcctgcg gcggcgactc tggcgcgaca gcgcacgctc gatttcctgc gccagcacgt
     2701 cggctgaagc gttctggccc gagccgccga ttagttgaca aaagcaacta atcggccgga
     2761 cagtggcgtc gacggccgca tcctaaaggc cgaagggcga gcgatgccca caacggcggc
     2821 gaacaaggcg gtgcgcgagg cgcggattgc ggcgctgcgc cgattcaatc gcttttacac
     2881 gcagcgcatc ggtgtactgc aggaaggtct gctcgacagc ccgttctcgc tggcgcaggt
     2941 gcgcgtcctc tacgagctcg cgcatcccga atcgtacggc gacgacgggc cgaccgcgag
     3001 cgagctctcg gcaaggctgg gcgtcgacga aggctatctg agccgcatcg tgcacgggtt
     3061 cgagtcgaag ggaatgctgt gccgtcgtcc cagcacggcc gacgcccgac ggcagttgct
     3121 ggcgctgact cgcgaggggc gggcggcgtt cgcgccgctc gaccggcaat cgcacgatga
     3181 ggccggtgcc ctgctggata ccctttcggc cggcgaccag cgtcgcctgg tcgcggcgac
     3241 ggacgcgatc gagagcctgc tcgaccggag tgcggtgccg cgacgggcga ttgcattgcg
     3301 cgcgccgcgg ccgggcgaca tcggctgggt cgtgcagcgg catggcgagt tgtatgcgcg
     3361 cgaatacggc tacgatgccc aattcgaggc gctcgtcgcc ggcatcgccg cccggttcgt
     3421 gcagcgtttc gacgccgggc atgaacgttg ctggatcgcc gactgcggcg gcacgaacct
     3481 gggctcggtg tttgtcgtgc ggcagtcggc tcacgtcgcc aagctgcgcc tgctgctggt
     3541 cgaccccgcg gcgcgcggcc gaggtctcgg cgcgcgcctg gtcgcggaat gcatccgttt
     3601 cgcgcggcgc gccggttatc ggagcatggt gctgtggacg caaagcgagc tcgccgcggc
     3661 gcggcatatc tacgagcagg ccggcttccg acgcacccgt cgccaggcgc accacagttt
     3721 cggccgcgac ctggtcgggg aaacatggcg cctcatgctc tagcgcgccg ctggcggggg
     3781 tgggccgcga cagggcgctt ggcgccgggt tacacttgac cggcccaacc ggcggcaaac
     3841 cgccgcgcag caaccgtggg cggcatcgcc tgccgccttg tcgacggcca acggaggacg
     3901 gacgatgaaa tgcacgacct gcggttacga aacgcagccg ggcgccagat tttgcgtcca
     3961 gtgcgggacg accctgccgg ccgaaccggt gcccgtcgcg gcggctgccg ctgccgcggc
     4021 cacgcccgtc gccccatccg cgccggcg
//
LOCUS       DATANS010000438         1909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_526320, whole genome shotgun sequence.
ACCESSION   DATANS010000438 DATANS010000000
VERSION     DATANS010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526320"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VHT22_06420"
     CDS             <1..208
                     /locus_tag="VHT22_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX3137223.1"
                     /translation="NPLAEQKGSPIYGMPIIEAYKAKSVVVNKRSMASGYAGLDNPLF
                     YMDKTMMVFGDAKKIVEDLVKAVD"
     gene            259..1503
                     /locus_tag="VHT22_06425"
     CDS             259..1503
                     /locus_tag="VHT22_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX3137224.1"
                     /translation="MPGPLAHLTVVDLSRVLAGPWCTQLLADLGAEVIKIEKPGGGDD
                     TRRWGPPFLKDANGRDTAEAAYYLGCNRGKQSVAVDFTSTEGRRIVQDLCRQTDIVVE
                     NFKVGALARYGLDYPAIAAINPRLVYCSITGFGQTGPYADRAGYDFIIQGIGGFMSVT
                     GERDDRPGGGPQKAGIAIADLMTGMYATTAILAAIAHRAVAGVGQYIDTALLDAQVAM
                     MASMDMNYLITGTPPGRAGNAHQNIVPYQVFDCADAPLVLAVGNDGQFARFCEVAGQT
                     AWLDDPRFATNIERVRHRDFVVPLIAELMRTRAQRDWLTPLDAAGVPCGPINLLDQVF
                     ADPQVIARGMRIDMPHPLAGSVPQVGNPLKFSATPVAYTRAPPLLGEHTREVLSARLG
                     LADAALEELAGRGVIQLSELRT"
     gene            1565..>1909
                     /locus_tag="VHT22_06430"
     CDS             1565..>1909
                     /locus_tag="VHT22_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137225.1"
                     /translation="MNGITRKPAFWIVFAVISALSGVFAWRYFPAALPLINLDVKMTR
                     GDALAEAAAIANRLNLAPAGAQRAALFAHDGATQNFVELEAGGKPAFSQMLNGTLYSP
                     YWWEVRLFKPGET"
BASE COUNT          329 a          631 c          618 g          331 t
ORIGIN      
        1 caatccgctg gccgagcaga agggcagtcc gatctacggc atgccgatca tcgaggcgta
       61 caaggccaag tcggtggtcg tcaacaagcg ctcgatggcc tcgggctacg ccgggctcga
      121 caatccgctg ttctacatgg acaagacgat gatggtgttc ggcgacgcca agaaaatcgt
      181 cgaggatctg gtcaaggccg tcgactaggc cctggtcgcg gcgcgaccgc aacgactcca
      241 ccggcccgga gatcatcgat gccgggaccg ctcgctcacc tgaccgtcgt cgatctgtcg
      301 cgcgtgcttg ccggcccgtg gtgcacgcag ctgctggcgg acctcggcgc cgaagtgatt
      361 aaaatcgaaa agccgggcgg cggcgacgac acgcgccgct ggggtccgcc gttcctgaag
      421 gacgcgaacg ggcgcgacac cgccgaggcc gcctattatc tgggctgcaa tcgcggcaag
      481 cagtcggtcg ccgtcgactt cacgtcgacc gaaggccggc gcatcgtcca ggacctctgc
      541 cgccagaccg acatcgtcgt cgagaatttc aaggtcggcg cgctcgcccg ctacggcctc
      601 gattaccctg cgatcgccgc gatcaatccg cggctggtct actgctcgat caccggattc
      661 ggccagaccg gcccgtacgc ggaccgcgcg ggctacgatt tcatcatcca gggcatcggc
      721 ggcttcatga gcgtcaccgg cgagcgcgac gatcggccgg gcggcgggcc gcagaaagcc
      781 ggcatcgcaa tcgccgacct gatgaccggc atgtatgcga cgacggcgat cctggcggcg
      841 atcgcgcatc gcgcagtcgc cggtgtcggc cagtacatcg atacggcgct gctcgatgcg
      901 caggtcgcga tgatggcctc gatggacatg aattatctga tcaccggcac gccgccgggc
      961 cgcgccggca acgcgcatca gaacatcgtg ccgtaccagg tattcgattg cgccgacgcc
     1021 ccgctggtcc tggccgtggg caacgacggg cagttcgcgc gcttttgcga agtcgctggc
     1081 cagaccgcgt ggctggacga ccctcgtttc gcgaccaaca tcgagcgcgt gcgccatcgc
     1141 gacttcgtcg tgccgctgat cgccgagctg atgcgcacac gggcgcagcg cgactggctg
     1201 acgccgctgg atgccgccgg agtgccctgc gggccgatca atcttctcga ccaggtgttt
     1261 gccgatccgc aggtgatcgc gcgcggcatg cgcatcgaca tgccacatcc tttggccggc
     1321 tctgtgcctc aagtcggcaa tccgttgaaa ttctcggcga cgccggtcgc gtatactcgg
     1381 gcaccgcctt tgctcggcga gcacacgcgg gaggtgcttt cggcgcgact gggcctggcg
     1441 gatgctgctt tggaagaact cgccgggcgc ggcgtaatcc aactgagcga actgcgaacg
     1501 tgaccacgcc gtaagcccga tctcaagcgc tccggttttc ctcaatcagc gaccgaaacc
     1561 agcaatgaac ggaatcaccc gcaagccggc gttctggatt gtctttgccg tcatctcggc
     1621 cttgtcaggt gtatttgcct ggcgctattt tccggcggca ctgccgctga tcaatctcga
     1681 cgtcaagatg actcgaggcg atgcgttggc cgaggccgcc gcgatcgcaa accgtctgaa
     1741 tctcgcgccg gccggcgcgc aacgcgcggc gctgttcgcg cacgacggcg cgacgcagaa
     1801 cttcgtcgag ctggaggccg gtggcaagcc ggcgttttcg caaatgctca acggtacgct
     1861 ctactcgccg tactggtggg aggttcggct gttcaagccg ggcgaaacc
//
LOCUS       DATANS010000439         2749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_568131, whole genome shotgun sequence.
ACCESSION   DATANS010000439 DATANS010000000
VERSION     DATANS010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568131"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VHT22_06435"
     CDS             complement(<1..1029)
                     /locus_tag="VHT22_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HEX3137226.1"
                     /translation="MAKVQLRDVRKSYANLEVIHGVSIDIADGEFIVIVGPSGCGKST
                     LLRMVAGLEAITGGEIVIGDRVVNTLEPKDRDIAMVFQNYALYPHMSVFGNMAYGLKI
                     RGFKKADIDARVKRAAEILELGPMLERKPRQLSGGQRQRVAMGRAIVREPAVFLFDEP
                     LSNLDAKLRVQMRFEIQKLHRRLGTTSLYVTHDQVEAMTLAQRMIVMNAGRAEQIGTP
                     MEVYQNPATVFVAGFIGSPAMNFIEGKSEGDGRIQLEGSGVLVVPKAPPAGRKLTIGI
                     RPEHLTPCKPSEANLVGSVEMIEALGADTLIHVAVAGHTIIARLPNGTPAEIGEPIAL
                     AADRDRIYL"
     gene            complement(1042..1887)
                     /gene="ugpE"
                     /locus_tag="VHT22_06440"
     CDS             complement(1042..1887)
                     /gene="ugpE"
                     /locus_tag="VHT22_06440"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HEX3137227.1"
                     /translation="MIENRPLLTWISYIVLTIGVAIVAFPVYVTLVASTRTAQEVLQA
                     PMPLTPGTHMLENYAAVLAHGAGESGAGVGRMMVNSLISALVIAIGKIAISLLSAFAI
                     VYFRFPFRRFFFWMIFVTLMLPVEVRILPTYKVVADLGMLDTYAGLTIPLIASATATF
                     LFRQFFLTIPDELAEAARIDGAGPMRFFWDVVLPLSRPNIAALFVILFIYGWNQYLWP
                     LLVTTQEDMYTTVIGIKRMISGGDAATDWNLVMATAMLAMLPPALVVLLMQRWFVKGL
                     VDAEK"
     gene            complement(1900..2748)
                     /gene="ugpA"
                     /locus_tag="VHT22_06445"
     CDS             complement(1900..2748)
                     /gene="ugpA"
                     /locus_tag="VHT22_06445"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HEX3137228.1"
                     /translation="MPYALLLPQVIVTIVFFFLPAGQALIQSTQIQDAFGGSLQFVGL
                     DNFRDLFNDALYLESFKVTALFSILVAGLGLTISLILAVFADRVVRGAGVYKTLLIWP
                     YAVAPAIAAVLWLFLFNPTLGIVAHWLTSAGVDWNYLLYGNQALMLVVIAAVWKQVSY
                     NFLFFLAGLQSIPKSLLEAAAIDGAGPVKRFATIVFPLLSPTTFFLLVVNIVYAFFDT
                     FGIIDAATSGGPGQATEILVYKVYKDGFKGLDLGGSAAQSVVLMTIVITLTVIQFRYV
                     ERKVQY"
BASE COUNT          535 a          864 c          882 g          468 t
ORIGIN      
        1 caggtaaatg cggtcgcgat cggcggccag cgcgatcggc tcgccgattt cggccggcgt
       61 gccattcggc aggcgcgcga tgatcgtgtg tccggcgacg gcgacgtgga tcagggtgtc
      121 ggcgcccagc gcctcgatca tctcgacact gccgaccaga ttggcttcgc tcggcttgca
      181 cggcgtcagg tgttcgggcc ggatgccgat cgtcagcttg cgtccggcgg gaggagcctt
      241 cggcacgacc agcacaccgc tgccttcgag ctggatgcgt ccgtcgccct cgctcttgcc
      301 ctcgataaag ttcatcgccg gtgagccgat gaagcctgcg acgaagaccg tcgccggatt
      361 ctgatagacc tccatcggcg ttccgatctg ctcggcccgg ccggcgttca tcacgatcat
      421 tcgctgcgcc aaggtcatcg cctcgacctg gtcatgcgtg acgtacaggc tggtcgtgcc
      481 gagacggcga tgcagcttct gtatctcgaa gcgcatctgc actcggagct tggcgtccag
      541 gttcgacaag ggctcgtcga acaggaaaac cgcgggctcg cgcacgatcg cgcggcccat
      601 cgcgacgcgc tggcgctgtc cgccggaaag ctggcgcggc ttgcgctcga gcatcggccc
      661 gagctcgagt atttcggcgg cgcgcttgac cctggcgtcg atgtcggctt ttttgaagcc
      721 gcggatcttc aggccgtacg ccatgttgcc gaacacgctc atgtgcgggt acagcgcgta
      781 attctggaac accatcgcga tgtcgcggtc cttcggctcc agcgtgttga cgacgcggtc
      841 gccgatgacg atctcgccgc ccgtgatcgc ttccagcccg gcgaccatgc gcagcagggt
      901 cgatttgccg cagcccgatg ggccgacgat gacgatgaac tctccgtcgg cgatgtcgat
      961 cgacacgccg tgtatgacct cgagattggc gtaactcttg cgcacgtcgc ggagctggac
     1021 tttagccatt gttccctgcc gtcatttctc ggcgtcgacc agacccttga cgaaccagcg
     1081 ctgcatcagc agcacgacca gcgccggcgg cagcatcgcc agcatcgcgg tcgccatcac
     1141 caggttccag tcggtcgcgg cgtcgccgcc cgagatcatt cgcttgatgc cgatcaccgt
     1201 cgtgtacatg tcttcctgcg tcgtcaccag caatggccac aggtactggt tccagccgta
     1261 aatgaaaagg atcacgaaca gcgcggcgat attgggccgc gacagcggca ggacgacgtc
     1321 ccagaaaaag cgcatcggcc ccgcgccgtc gatgcgcgcc gcctcggcga gctcgtcggg
     1381 tatcgtcagg aagaactgcc ggaacaggaa cgtcgccgtg gccgaggcga taagcggaat
     1441 cgtgagcccg gcgtaggtgt cgagcatacc caggtcggcg acgaccttgt acgtcggcag
     1501 gatgcgcacc tcgaccggca gcatcagcgt gacgaagatc atccagaaga agaatctccg
     1561 gaatggaaag cggaaataga cgatggcgaa tgccgacagc agcgaaatcg cgattttgcc
     1621 gatcgcgatg accagcgccg agatcaggct gttgaccatc atccggccga cgccggcgcc
     1681 ggattcgccg gcgccatgcg ccagcacggc ggcgtaattc tccagcatgt gggtgcccgg
     1741 cgtcagcggc atcggcgctt gcagcacttc ctgcgcggtg cgggtcgatg caaccagggt
     1801 cacgtacacc ggaaacgcga cgatcgcgac gccgattgtc agcacgatgt agctgatcca
     1861 ggtgagcagc ggccggttct cgatcatgcg tttcagggcc tagtactgca ccttgcgttc
     1921 gacgtaacgg aactggatca ccgtcaaggt gatgacgatg gtcatcagca ccaccgactg
     1981 cgccgccgag ccgcccagat ccagcccttt gaagccgtcc ttgtacacct tgtagacgag
     2041 gatctcggta gcctgtcccg gcccgcccga cgtcgcggcg tcgatgatgc cgaacgtatc
     2101 gaaaaaggcg tagacgatat tgaccaccag caggaaaaac gtcgtcgggg acaacagcgg
     2161 aaagacgatc gtcgcgaagc gcttgaccgg ccccgcgccg tcgatcgccg cggcctcgag
     2221 cagcgacttg gggatcgatt ggaggccggc gagaaagaac aggaagttgt agctgacctg
     2281 cttccagacc gcggcgatga cgacgagcat cagcgcctgg ttgccgtaca ggaggtaatt
     2341 ccagtcgacg ccggcgctcg taagccagtg ggcgacgatg cccagcgtcg gattgaacag
     2401 gaacagccac aggacggcgg cgatcgccgg agcgacggcg tacggccaga tcagaagcgt
     2461 cttgtacacg cccgcaccgc ggacgacgcg atcggcgaac accgcgagga tcagcgatat
     2521 cgtcagcccg agtccggcca ctaggatcga aaatagcgcc gtgaccttga acgactcgag
     2581 atagagcgcg tcattgaaca gatcgcggaa attgtccagg ccgacgaact gcaggctgcc
     2641 gccgaaggca tcctggattt gcgtcgactg gatcagcgcc tgccccgcgg gcaggaagaa
     2701 aaatacgatg gtgacgatga cctgcggcag cagcagcgcg tagggcagc
//
LOCUS       DATANS010000440         1571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_526398, whole genome shotgun sequence.
ACCESSION   DATANS010000440 DATANS010000000
VERSION     DATANS010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526398"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1571)
                     /gene="prsT"
                     /locus_tag="VHT22_06450"
     CDS             complement(<1..>1571)
                     /gene="prsT"
                     /locus_tag="VHT22_06450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HEX3137229.1"
                     /translation="ISPLRALAALYMRTGRTAQGIETLEAALARAPDNPVLLQAAGEA
                     NLAAGNAAKAAQYYERASALDKGNMASKVRLAQVKYAAGDPGQAFKELESLSGADQSQ
                     YQADLALIAAHLRRGEFDQALTAVAALEKKQPNNPLTYNVKGAAYAGMHDNKNARASF
                     EKALDVQPGYFPAARNLALLDVQEQKAEDARKRYEAMLAKDPKNEQLLLGLAELLVLS
                     GRSPDEVRATIDRAIAAAPKSVRPRLALINYYGNLRDAKAGLEAAKAAQAAFPDDVQI
                     LEAVGAAQRAAGQADEALATFKRLVQLQPQNATASLRLADVQVAKKDYSAAIATLGNV
                     ISAEPDNNRALVALAKTYVMSGHPEDAIAEARKLQKDRPKRAIGYALEGEVLAAQQKW
                     QQAAAAYRDALAREPIPILAVRRYEALQKVGAADASAFAATWTREHPTDVTLVIFLAQ
                     QSLARKDYRAAISNYQAAVKLDPDNAMVLNNLAWALAETGDPKAREYASAAYRQAPFN
                     PNVIDTLGWAEVQIG"
BASE COUNT          209 a          520 c          517 g          325 t
ORIGIN      
        1 cgccgatttg cacttcggcc cagcccagcg tatcgattac attcggattg aacggcgcct
       61 gccggtacgc cgctgaggca tattcgcgtg ccttcggatc gccggtttcc gccagcgccc
      121 aggcgaggtt gttcaagacc atcgcgttgt cggggtcgag cttcacggcc gcctgatagt
      181 tgctgattgc cgcacggtaa tccttgcggg caaggctctg ctgcgcgagg aaaatgacca
      241 gcgtgacatc ggtcggatgc tccctcgtcc atgtcgcggc gaacgcgctg gcgtcggcag
      301 cgccgacttt ctgcaatgcc tcgtagcggc gcaccgcgag tatcgggatc ggctcgcgtg
      361 caagcgcatc ccggtacgcg gcggcagcct gctgccactt ctgctgcgcg gccagcactt
      421 ctccttcgag cgcgtagcct attgcgcgct tcggacggtc tttctgcagc ttgcgcgcct
      481 cggcgatggc gtcttcggga tgaccggaca tgacataggt cttggccagg gccaccagag
      541 cgcggttatt atcgggttcg gccgagatga cgttgcccag ggttgcgatg gcggcgctat
      601 agtctttctt cgcgacttgc acgtcggcca ggcgcagcga tgccgttgcg ttctgcggct
      661 gcagctgcac caggcgcttg aacgtggcca gtgcctcgtc ggcctggcca gcggcgcgct
      721 gcgcggctcc taccgcctcc aggatctgca cgtcatcggg aaaagccgcc tgtgccgcct
      781 tggcggcctc gagccccgcc ttggcatcgc gaaggttgcc gtagtagttg atcaatgcca
      841 gccgcggccg caccgacttg ggcgctgcgg caatcgcacg gtcgatcgtc gccctgactt
      901 cgtccgggct gcgccccgac aggaccagaa gctcggccaa gcccagcaac agctgctcgt
      961 ttttcggatc cttggccagc atcgcctcgt agcgcttgcg cgcgtcctcg gctttctgct
     1021 cctgcacatc gagcagcgcg aggttgcgcg ctgcgggaaa atatcctggc tgcacgtcca
     1081 gcgccttctc gaagcttgcc cgcgcattct tgttgtcgtg catgccggcg tatgccgcgc
     1141 ccttgacgtt ataggtcagc gggttgttcg gttgcttttt ttccagcgcg gcgaccgcgg
     1201 tcagcgcctg gtcgaattca ccgcggcgca aatgcgcggc gatcaacgcg aggtccgcct
     1261 ggtactgcga ttgatcggcg cctgacaggg actcgagttc cttgaacgcc tgtcccgggt
     1321 cgccggcggc gtacttgacc tgggccagcc gaaccttgct cgccatgttg cccttgtcca
     1381 gcgcgcttgc ccgctcgtag tactgcgcgg ccttggcggc gtttccggcg gcgaggttgg
     1441 cctcgccggc tgcctgcagc aggaccggat tgtccggcgc gcgcgccagc gccgcttcga
     1501 gcgtttcgat gccctgggcg gtgcgtccgg tgcgcatgta cagggcggcg agcgcgcgca
     1561 gcgggcttat g
//
LOCUS       DATANS010000441         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_443862, whole genome shotgun sequence.
ACCESSION   DATANS010000441 DATANS010000000
VERSION     DATANS010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443862"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VHT22_06455"
     CDS             <1..716
                     /locus_tag="VHT22_06455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3137230.1"
                     /translation="LGQGAEQMIRDSAALAQTPESRALRAHMVLRSRFAEDRLAAAVA
                     RGVTQYVILGAGLDTFAVRQPGWARNLRILEVDHAGTQELKRSHLAAAGLTVPPNAGF
                     ATIDFERESVREGLLRHAISPTQRTFFSWLGVTMYLREDAIDAVLRSVAEFPSGSEIV
                     LTFASTSNMPQGVADRAAQAGEPWLSYFEPEQIEDKLRAAGFTTVELLLPLVAKSRYF
                     NAPTSLPTPVRTTIVAAVR"
     gene            727..>1569
                     /locus_tag="VHT22_06460"
     CDS             727..>1569
                     /locus_tag="VHT22_06460"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX3137231.1"
                     /translation="MVAPVTHHSKPAADIEIDETLVRSLLRAQHPDLAHLPLQSMDSG
                     WDNAMFKLGDKLAVRLPRRVAVAKLIEHEQRWLPQLAPLLPIAVPAPVRVGEPAENYP
                     ARWSVVPWLHGRNADLSEPRSDQAERLAAFLCALHRPAPADAPFNPYRGVPLRERAEQ
                     IVERIRRLKQETTLLNDTIVRIYEDAIETPIDVEPTWLHGDLHGGNLLVDDNGVITAV
                     IDWGDMTRGDRATDLATLWLNLGNRKARENAMRACHGVSAATWRRAKGWAVFYGVTLG
                     TSGLA"
BASE COUNT          266 a          528 c          518 g          257 t
ORIGIN      
        1 tgctcgggca gggcgccgag caaatgatca gagactctgc cgcgctcgcg caaacgcctg
       61 aaagtcgagc cctgcgcgcg cacatggtct tgcgatcgcg ctttgccgaa gaccggctcg
      121 ccgccgccgt cgctcgcggc gtgacgcaat atgtcatcct cggcgccggc ctcgacacgt
      181 tcgctgtgcg tcagcccggg tgggcgcgga atctgcgcat cctcgaagtc gatcacgccg
      241 ggacgcagga actgaagcgc tcgcacctcg cggcggccgg gctcacggtg ccgccgaatg
      301 cagggtttgc gaccatcgac ttcgagcgcg agtcggtgcg cgagggactg ctgcgtcatg
      361 caatctcgcc gacgcagcgc acgttctttt cctggctcgg cgtcaccatg tatctgcgcg
      421 aagacgccat cgatgcggtg ttgcgctcgg tcgcagagtt tccgtcgggc agcgagatcg
      481 tgctgacgtt cgcgtccacc agcaacatgc cacaaggggt cgcggaccgg gccgcgcaag
      541 cgggcgagcc gtggctgagc tacttcgagc cggagcagat cgaggacaag ctccgcgcgg
      601 cgggcttcac gaccgtggag ctgctgttgc cgctcgtcgc gaagagtcgc tacttcaacg
      661 cacccacctc gctgccaacg ccagtgcgaa cgaccatcgt cgcggccgtg cgttagaatc
      721 cgcgcggtgg tcgcgccagt cacccatcac agcaagcccg cagcggacat cgagatcgac
      781 gagacgttgg tccgctcgct gctgcgcgca caacatccgg acctcgcgca cttgccgctg
      841 cagtcgatgg actcgggctg ggacaacgcg atgttcaagc tcggcgacaa actcgccgtg
      901 cgcctgccgc ggcgggtcgc agtcgcgaag ctgatcgagc acgagcaacg ctggctgcca
      961 cagctcgcac cgctgttgcc gatcgctgtg ccggcgcccg tgcgcgtcgg cgagcctgcc
     1021 gaaaactacc cggcgcgctg gagtgtcgtg ccgtggctgc acggacgcaa cgccgatctg
     1081 agcgagcctc gatccgatca agccgagcgg cttgctgcgt ttctctgcgc gctccatcgc
     1141 cccgctccgg ccgacgcgcc cttcaatccc tatcgcggcg tgccgctgcg ggaacgggcc
     1201 gaacagatcg tggaacggat ccgtcgcttg aagcaggaga cgacgctgct caatgacacc
     1261 atcgttcgca tctacgagga tgcaatcgag acgccgatcg acgtcgagcc gacgtggctg
     1321 cacggcgacc tgcacggcgg caacctgctc gtcgatgaca acggcgtgat cactgcggtg
     1381 atcgattggg gcgacatgac tcgcggcgat cgcgcgacgg atctcgcaac actgtggctg
     1441 aatctcggca atcgcaaagc gcgcgagaac gcgatgcgtg catgccacgg cgtcagcgct
     1501 gcgacgtggc gacgtgcgaa aggttgggcg gtgttctacg gcgtaacgct cggtacttcc
     1561 ggcctcgcc
//
LOCUS       DATANS010000442         7432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_696161, whole genome shotgun sequence.
ACCESSION   DATANS010000442 DATANS010000000
VERSION     DATANS010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7432
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696161"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1152
                     /locus_tag="VHT22_06465"
     CDS             187..1152
                     /locus_tag="VHT22_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX3137232.1"
                     /translation="MPTARGRLIALAAGLCLAGCGPPAERALQGYVEGEYVRVAAPFA
                     GTLQKLAVQRGAQVASGAPLFALESDNEIAARRQAEQQLQAAEARLANLMTGKRAPEV
                     EMVAEQLRQAQAARELSAANLRRQEALFKSGFISKAALDDVRTQLQRDDAAVSQLQAS
                     VATARLPARPDEIRAAEADAAGARQALAQADWRLSQRAVAAPTAAHVSDTYYVVGDWV
                     PAGGVVASLLPAANVKIRFFVPETELGRLKPGQTVNFNCDGCGVPMSATVNFISDRAE
                     FTPPVLYSKENRAKLVYLVEAKPAADVAAKLNAGQPVDVVLPRSN"
     gene            1156..1833
                     /locus_tag="VHT22_06470"
     CDS             1156..1833
                     /locus_tag="VHT22_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX3137233.1"
                     /translation="MNAGITIEATFCFVDIAGYTALTDTHGEHAAADLVDEFGAMVRA
                     SIEPSGRLQSLTGDCAFLVFPDPLVANDALSRLYMAIADRRNFPVVRAGLHHGAALLR
                     EDRHFGSTVNLAARVAAHATGGQILCTRQVADALIEAQTQGVEIEHQGSFSMKNLPHP
                     VDLYEVVLSGCAREYAIDPVCKMQVDKRRAAGDLHFEKRTYWFCSLACVERFARQPLS
                     YVSTEKQ"
     gene            1830..2825
                     /locus_tag="VHT22_06475"
     CDS             1830..2825
                     /locus_tag="VHT22_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3137234.1"
                     /translation="MTTGGQGTTNSDVVIDVRGLNKHFGANHAVKDLTLTVRRGEIFG
                     FLGPNGSGKTTSIRMLCGLLTPDSGEGTCLGYDIRQQTREIKRHVGYMTQRFSFWEDL
                     TIRENLRFIARMYGMPDGAARVDQALEHLGLASRAKQLAGSLSGGWKQRLALAACLLH
                     DPQLLLLDEPTAGVDPKARRDFWEELHALAARGITVLVSTHYMDEAERCHKLGYILYG
                     KLLVQGTAEEVIASQSLTTWEVTGGDLPALAERLRSIAAVEQVAAFGNALHVTGSNRD
                     ALKSALAPLMNEAGHEWREVEPGLEDVFIHLMRDADDAPAAAETSTTAAPKVQAK"
     gene            2822..3961
                     /locus_tag="VHT22_06480"
     CDS             2822..3961
                     /locus_tag="VHT22_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016726817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3137235.1"
                     /translation="MNGGLFSFSRWLGIVGKEFIQLKRDRLTFGMIVGIPVVQLVLFG
                     FAINSDPKRLPTALLVADRSEFSRSILAGLQNSSYFDFIGEAKDEDDADRMLAVGKAQ
                     FVVSIPADFSRRLVRGERPAMLIEADATDPTATGNALSAVNQLAQTVLSRDLAGSLSP
                     LAGTPSAFSIIVHPRYNPEAVTQYNIVPGLMGVILTMTMVMMTGLAITRERERGTMEN
                     LLATPATVLEVMTGKIVPYILIGLLQVTIVLVLARALFSVPIQGSITVLYLSVLLFIA
                     ANLMLGLTISSIARNQLQAMQMTFFFFLPSMLLSGFMFPFRGMPGWAQLIGEALPLTH
                     FLRIVRGVLLKANGWAEIWPEAWPIALFGFVIMAIGLRTFRSTLD"
     gene            4026..4544
                     /locus_tag="VHT22_06485"
     CDS             4026..4544
                     /locus_tag="VHT22_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3137236.1"
                     /translation="MTEPLHESIPAAGDAAYRHGDSGTGFPPRYEGPRMLAQALTRLA
                     ISEQGFTRDGPRADVEYRDLGWAAASGGTIGAKHIRAVLPFEAETGWHWHDMSAHFVY
                     VLRGWIEFRYAGVVETVRVSAGSSLSQPAGVAHNVVDRSDDLELIEINLPAGYGTWAL
                     RAEPGAADGPRE"
     gene            4541..5401
                     /locus_tag="VHT22_06490"
     CDS             4541..5401
                     /locus_tag="VHT22_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3137237.1"
                     /translation="MSLDPVAPVVADASATPVAAAAGDQLAVAAPPPTKARDRISAVL
                     LPIATFAVLLVAWQFLVRLFGVPEYILPLPTEFLSKLVESRALIWQHTLVTASEVLLG
                     FLFAAAIGVPLGLMIVSFKWLERSLYPLIVFFQLIPKIAVAPLFVVWFGFGPFPKVLL
                     TFLLCFFPTLVASMTGFRALDERVLYLTRSMGANPGQTFRYIRLPSAIAHIFGGFKVS
                     IVFAATGAIVAEFVGSNSGLGYLLLRATSYLDMPLIFAVLVALSAIGIVFSYAVQLLE
                     RLCMPWLHGN"
     gene            5436..6425
                     /locus_tag="VHT22_06495"
     CDS             5436..6425
                     /locus_tag="VHT22_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3137238.1"
                     /translation="MIKKLFAAALAAVLASAALPAAAQQAKPMRLTLNFLAGGPQAGF
                     MLAKKLGYYKDVGIDLTIEEGKGSATTAQQVATGQTDVGFADAPAAMQIRSKGAPLKI
                     IAPILQTNGFAIISIEGSGIKSPKDLVGKKLAVQPGTAQTTLLDAILAENKLDKTKLD
                     IVNIDPAALVGALLEKKVDAILAGADFQSVQMRDRGFKVNDIFYRDVGVPTVGLSIVA
                     RDDRLKESPDLYRNFVAASLKGWDEARKNPDAAAAAVVEQFPSASKDQILKQLNVDLL
                     LVCAPGATSLGRVPDKNWQTTFDLLTMYLNLPKDKPIGDYYSNDFLPASAPKC"
     gene            6488..7249
                     /locus_tag="VHT22_06500"
     CDS             6488..7249
                     /locus_tag="VHT22_06500"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3137239.1"
                     /translation="MSRAVGKRFAGKGGMFTALEDVSFDIRGGEFVSLIGPSGCGKST
                     LMLITAGLVAASTGELIVGGEVLQRPLTDVGIVFQDHLLLEFRSALDNVLLQAQIRRL
                     PLAAAREEAARILGKLGIPEPARGRYPRQLSGGMRQRVSIARALIHQPSILLMDEPFG
                     ALDAITRTQIRHDLELLWMETRKTVLFITHSIEEAIGLSDRVLVMSPNPGRIVDEIAI
                     DLPRPRPAVLGEDASFNAYAERIHRAFLRMGVIKY"
     gene            7252..>7432
                     /locus_tag="VHT22_06505"
     CDS             7252..>7432
                     /locus_tag="VHT22_06505"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX3137240.1"
                     /translation="MRTLVTGGAGFLGQAFAVRARRAGHQVTTVDLGGEADVQCDVTD
                     VPALAAAMAQVSPEVV"
BASE COUNT         1255 a         2397 c         2445 g         1335 t
ORIGIN      
        1 gcgccgctgc tgttgtcgga gctcctgagc aagcaggaag tgctggccgc gatcggcacg
       61 ctgcattcga gttagcatcc tcgctccggc gggagcgagg tggcggactg gccggtcggc
      121 gttcgctggc gttttccggg ccctgcggta tgcttcgccg gccacaacga ctcaacggag
      181 acaacgatgc cgaccgctcg cggccgcctg atcgcgctcg ccgccggcct gtgtctcgcg
      241 ggctgcggac cgccggcgga gcgtgcgctg caagggtatg tcgagggcga atacgtgcga
      301 gtcgcggcgc cgttcgcggg cacgctgcag aagctggcgg tgcagcgagg tgcgcaggtc
      361 gcttccggcg cgccgctgtt tgcgctggaa agcgacaatg aaatcgcggc acgccgccag
      421 gccgagcagc agctgcaggc ggcggaggcg cggcttgcca atctgatgac cggaaagcgc
      481 gcgcccgaag tcgagatggt cgccgagcaa ttgcgtcagg cccaggcggc acgcgagctt
      541 tccgcggcga acttgcggcg tcaggaagcg ctgttcaagt cggggttcat cagcaaggca
      601 gcgctcgatg acgtgcgcac gcaattgcag cgcgacgatg ccgcggtttc gcaactgcag
      661 gcgtcggtcg cgacggccag gctgccggcg cgtccggacg agatccgcgc cgccgaagcc
      721 gatgccgccg gcgcgcgtca ggcactggcc caagccgact ggcgcttgtc gcagcgcgcg
      781 gtcgcggcac ccacggcggc gcacgtcagc gatacctact acgtcgtcgg cgactgggtt
      841 ccggcgggcg gtgtcgtcgc cagcctgctg cccgcggcca acgtcaagat ccgtttcttc
      901 gtccccgaaa cggagctggg acgactcaag ccggggcaaa ccgtcaattt caactgcgac
      961 ggctgtggcg tcccgatgag cgcaacggtg aacttcatct ccgaccgcgc cgaattcacg
     1021 ccgccggtgc tgtactcgaa ggagaatcgc gccaagctgg tctacctggt cgaagcgaag
     1081 ccggcggcgg acgtggcggc gaagctcaac gcgggccagc cggtcgatgt ggtgctgccc
     1141 aggtccaact agacgatgaa cgcaggcatc accatcgaag cgaccttctg cttcgtcgac
     1201 atcgccggct acactgcgct gaccgatacc cacggcgagc acgcagcggc ggatcttgtg
     1261 gacgagttcg gtgcaatggt ccgggcttcg atcgaaccct cgggtcgact gcagtcgctg
     1321 acgggcgact gcgcgttcct cgtgtttccc gacccgctgg tcgccaacga tgcgctgtcg
     1381 cgactctaca tggcgatcgc cgatcgccgg aacttcccgg tggttcgcgc cggcctgcat
     1441 catggcgccg cgttgctgag agaagatcgt cacttcggat cgacggtgaa cctcgcggct
     1501 cgggtcgccg cgcacgcgac cggcggccag atcctgtgta ccaggcaggt tgccgatgcg
     1561 ctgatcgagg cgcaaacgca gggcgtcgaa atcgagcatc agggctcgtt ttcgatgaaa
     1621 aacctgccgc atccggtcga tctgtatgaa gtcgttctgt ccggctgcgc gcgcgaatat
     1681 gcgatcgatc cggtttgcaa gatgcaggtc gacaagcgtc gggccgccgg cgaccttcat
     1741 ttcgaaaagc gaacctactg gttctgttcg ctagcctgcg tcgagcgatt cgccagacag
     1801 cctctgtcgt acgtatcgac agagaaacag tgacgaccgg cgggcaaggt acgaccaaca
     1861 gcgacgtcgt catcgacgtg cgcggcctga acaaacattt cggcgccaac cacgcggtca
     1921 aggatctgac cttgacggtg cgccgcggcg aaatcttcgg ttttctcggc ccgaacggca
     1981 gcggcaagac gacgtcgatc cgcatgctgt gcggcctgct gacgcccgac agcggcgaag
     2041 gcacctgcct cgggtacgac atccggcagc agacccggga gattaagcgc catgtcggct
     2101 acatgacgca gcgcttttcg ttctgggaag acctgacgat tcgcgagaac ctgcggttta
     2161 tcgcgcgcat gtacggcatg cccgacggcg ctgcgcgggt cgaccaggcg ctcgagcatc
     2221 tggggctcgc gagtcgcgcc aagcagctag cagggtcgct gtcgggcggc tggaagcagc
     2281 gtctcgcgct cgccgcatgc ctactgcacg acccgcagct gctcctgctc gacgagccga
     2341 ccgccggcgt cgatccgaag gcccggcgcg atttctggga ggagttgcac gcgctcgccg
     2401 cgcgcggcat caccgtgctg gtcagcacgc actacatgga cgaagccgag cgctgccaca
     2461 agcttggcta catcctgtac ggcaagctgt tggtgcaagg gaccgcggag gaagtcatcg
     2521 ccagccagtc gctgacgaca tgggaggtca ctggaggcga cttgccggcg ctggccgagc
     2581 ggctccgcag catcgcggcg gtcgagcagg tcgccgcatt cggcaacgcg ctccacgtca
     2641 ccgggtcgaa tcgcgacgcg ctcaagagcg cgctcgcgcc gctgatgaac gaagcggggc
     2701 atgaatggcg cgaagtcgag ccgggtctcg aggatgtgtt catccacctg atgcgcgacg
     2761 cggacgacgc gccggcagcg gccgaaacgt cgacgacggc ggcgccgaag gtccaggcca
     2821 aatgaacggc ggcttgttct cgttttcgcg ctggctgggc atcgtcggca aggagttcat
     2881 ccagctcaag cgcgatcgcc tgacattcgg catgatcgtc ggcatcccgg ttgtgcagct
     2941 ggtcctgttc ggtttcgcca tcaattcgga tcccaagcgc ctgccgaccg cactgctggt
     3001 tgccgaccgc agcgaattct cgcgcagcat tctcgccggc cttcagaaca gcagctactt
     3061 cgacttcatc ggcgaggcaa aggacgagga cgatgcggac cggatgctcg ccgtcggcaa
     3121 ggcgcagttc gtcgtgagca taccggccga tttttcgcgc cggctggttc ggggagagcg
     3181 cccggcgatg ctgatcgagg ccgatgcgac cgaccctaca gcgacgggaa acgcgctgtc
     3241 cgctgtcaac cagctcgcgc agacggtgct gagccgcgat ctcgccggat cgctctcgcc
     3301 gctggccggg acgccgtccg cgttcagcat catcgtgcat ccgcgctaca accccgaggc
     3361 ggtcacccag tacaacatcg ttcccggcct gatgggcgta atcctgacga tgacgatggt
     3421 gatgatgacg gggctcgcga tcacgcgcga gcgcgaacgc ggcacgatgg agaatctgtt
     3481 ggcgaccccg gcgacggtgc tcgaggtgat gaccggcaag atcgtgcctt acatcctgat
     3541 cggcttgctc caggtgacga tcgtgctggt gctggcgcgc gcactgttca gtgtgccgat
     3601 ccagggcagc atcaccgtgc tctatctttc ggtgctgctg ttcatcgccg cgaatctgat
     3661 gctcgggctg acgatatctt cgattgcgcg caatcagttg caggcgatgc agatgacttt
     3721 tttcttcttc ctgccttcga tgctgctgtc gggattcatg tttcccttcc gaggcatgcc
     3781 cggctgggcc cagctgatcg gcgaagcgct gccgctgacg cacttcctgc gcatcgtgcg
     3841 cggcgtcctg ctcaaggcga acggctgggc ggaaatctgg cccgaggcat ggccgatcgc
     3901 gctgttcggc ttcgtcatca tggcaatcgg actcaggact ttccgcagca cactcgatta
     3961 gcgccattgc gccgccgctt gaccgctcgc ggcggggcgg cttattgtga tccggagttt
     4021 ccgcaatgac cgaacccctg cacgaatcga ttcccgctgc cggcgacgcc gcctatcgtc
     4081 atggcgacag cggcaccggc ttccctccgc gctatgaggg gccgcggatg ctcgcacagg
     4141 cgctgacccg tctcgctatc agcgagcagg gcttcacccg cgacggcccg cgcgccgacg
     4201 tcgaatatcg tgatctgggc tgggctgcgg cgagcggcgg cacgatcggc gccaagcaca
     4261 tccgggctgt cctccccttc gaggccgaaa ccggttggca ctggcacgac atgagcgcgc
     4321 atttcgtcta cgttctccga ggctggatcg agtttcgcta cgctggcgtg gtcgaaacgg
     4381 tgagggtgag cgccgggtcc tcgctgtcac agccggcagg cgtagcgcac aacgttgtcg
     4441 accggtcgga cgacctcgag ttgatcgaga tcaacctgcc cgccgggtac ggcacatggg
     4501 cgttgcgcgc cgagcccggc gccgccgacg gaccgcgcga gtgagcctgg atccggtcgc
     4561 gccggttgtg gccgacgcat cggcaacgcc ggtcgcggca gcggccggag accagctggc
     4621 ggtcgcggcc ccgccaccga ccaaggcgcg cgatcgcatt tccgccgtgc tgcttccaat
     4681 cgcgaccttt gccgtgcttc tggtggcgtg gcagttcctg gtgcggctgt tcggcgtccc
     4741 ggaatacatc ctgccgctgc ccaccgagtt cctgtccaag ctggtggagt cacgggcact
     4801 gatctggcag cacacgctgg tgacggcgag cgaggtcctg ctcggatttc tttttgcggc
     4861 tgcgatcggc gtcccgctcg ggctgatgat cgtgtcgttc aagtggctgg agcgatcgct
     4921 gtaccctctg atcgtttttt tccagctgat tccgaagatc gcggttgccc cgctattcgt
     4981 cgtctggttc ggattcggtc cgtttccgaa ggtgctgctc actttcctgc tctgcttttt
     5041 cccgacgctt gtcgccagca tgactggttt ccgggctctg gacgaacgcg tgctgtacct
     5101 tacgcgctcg atgggggcca accccggaca gacgttccgc tatatccgcc ttccgtcggc
     5161 aatcgcgcac attttcggcg gcttcaaagt ttcgatcgtc ttcgccgcca ccggcgccat
     5221 cgtcgccgag ttcgtcggat cgaactctgg cctgggctac ctgctgctgc gcgccaccag
     5281 ctatctcgac atgccgctga ttttcgcggt tctcgtagcg ctgtcggcga tcggcatcgt
     5341 gttcagctac gccgtacaat tgctcgagcg cctgtgcatg ccctggctgc acgggaattg
     5401 agcggaattc tcgataacca gaaccggagg agacgatgat caaaaaacta ttcgctgccg
     5461 cgcttgcggc ggtgcttgcc agcgctgcgt tgccggcggc ggcgcagcaa gccaagccga
     5521 tgaggctgac gctcaacttt ctcgccggcg gcccgcaggc cgggttcatg ctcgccaaga
     5581 agctcggcta ctacaaggac gtgggcatcg atttgacgat cgaagaaggc aagggctccg
     5641 cgaccaccgc gcaacaggtc gcgaccgggc agaccgacgt cggttttgcc gacgcgccgg
     5701 cagcgatgca gatccgttcc aaaggcgcgc cgctgaagat catcgcgccg atactgcaaa
     5761 ccaacggctt cgcgatcatc tcgatcgaag gctcgggcat caagtcgccc aaggatctgg
     5821 tcggcaagaa gctggccgtt cagccgggca ccgcgcagac gacgctgctc gacgcgatcc
     5881 tggccgaaaa caagctcgac aaaacgaagc tcgacatcgt caatatcgac ccggcagcac
     5941 tggtgggggc gctgctggaa aagaaagtcg atgcgatcct tgccggtgcg gattttcaga
     6001 gcgtccagat gcgcgatcgc ggattcaagg tcaacgatat cttctatcgc gacgtcggcg
     6061 tgccgaccgt cggcttgtcg atcgtggccc gcgacgaccg gctgaaggaa agccccgatc
     6121 tctaccggaa tttcgtcgca gcgagcctca agggctggga cgaagcgcgc aagaaccccg
     6181 atgcggcggc ggcagcagtc gtcgagcaat tcccgtccgc gagcaaggat cagatcctga
     6241 agcagctcaa tgtcgacctg ctgctggtct gcgcgcccgg cgcgacatct ttgggacggg
     6301 ttcccgacaa gaactggcag acgacgttcg acttgctgac gatgtatctc aatctgccga
     6361 aggacaagcc gataggcgat tattacagca acgatttcct gccggccagc gcgccgaagt
     6421 gctgagagtg caggctccga ccgcgatcga agcgcggccg tccgcggcgg tcggcggcgc
     6481 ttcgatcgtg agccgtgccg tcggcaagcg gttcgccggc aagggaggaa tgttcaccgc
     6541 actcgaggac gtgtcgttcg acatacgggg cggcgaattc gtcagcttga tcggcccctc
     6601 gggctgcggc aaaagcacgc tgatgctgat cacggcagga ctcgttgccg ccagcaccgg
     6661 cgagcttatc gtcggcggcg aagtgctgca gcggccgctg accgacgtcg gcatcgtctt
     6721 ccaggatcac ctgttgctcg agtttcgtag cgcgctggac aatgtgttgt tgcaggcgca
     6781 gattcgccgg ttgccgctcg cagccgctcg cgaggaggca gcgcgcattc tgggcaagct
     6841 cggcattccc gagcccgccc gcggacgcta tccgcggcaa ttgtccggcg gcatgcggca
     6901 gcgcgtgtcg atcgcccgcg ccctgatcca ccagccgtcg atcctgctga tggacgagcc
     6961 gttcggcgcg ctcgacgcga tcacgcggac ccagatccgg cacgacctcg agctcctctg
     7021 gatggagacg cgcaagaccg tgctgttcat cacccatagc atcgaagaag cgatcggcct
     7081 gtccgaccgg gtgctggtca tgtcgccgaa cccgggccgc atcgtcgacg agattgcgat
     7141 cgacttgccg cgtccacgtc cggcggtgct cggcgaggac gcgagcttca acgcctatgc
     7201 ggagcgcatc caccgcgcgt ttctgcgcat gggcgtcatc aagtactgac agtgcgaaca
     7261 ctggtaaccg gaggcgccgg cttcctcggc caagccttcg ctgtccgcgc gcggcgcgcg
     7321 ggacaccagg tgacgaccgt cgatctgggc ggcgaggccg acgtccaatg cgatgttacc
     7381 gacgttccgg cgctggcggc agcgatggcg caggtcagcc ccgaagtcgt cg
//
LOCUS       DATANS010000443         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_64190, whole genome shotgun sequence.
ACCESSION   DATANS010000443 DATANS010000000
VERSION     DATANS010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64190"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VHT22_06510"
     CDS             <1..917
                     /locus_tag="VHT22_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX3137241.1"
                     /translation="GIAALLRIAGRDAARASTYDLGFVVGPRLNAAGRLDDMAVGIEC
                     LITNDHGTAERIARQLDDLNRQRRAIESDMHENALASLAAITPEDGCSLCMFEPGWHQ
                     GVIGIVASRLKERFHRPVIAFARGDEGEIKGSGRSIGALHLRDALDLVAKREPDLILR
                     FGGHAAAAGLTLRESEFARFRAAFEIVANSLLSAADLERVIETDGALPLEHCTLEAAR
                     VLEHQVWGQGFAAPTFDDEFTVARQRVVGQRHLKLRLVKDNREVEAMLFAHATPLPDR
                     IHAVYRLAVNEWNGAQTLQLTIEHWQNA"
     gene            1011..2115
                     /gene="prfB"
                     /locus_tag="VHT22_06515"
     CDS             join(1011..1091,1093..2115)
                     /gene="prfB"
                     /locus_tag="VHT22_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293703.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX3137242.1"
                     /translation="MEAEQLNAIARRLSDLSQRNAELRRYLDFDAKQLRLAEVIKLAE
                     DPAVWSDPKRAQDLGRERKTLESLVTTMAKLDQDLRDTSELFTMARGEDDDATLKTIE
                     ADALALEKTVADVEFRRMFSNPMDPNNCFVDIQAGQGGTEAQDWTQTLERMYMRYCER
                     KGFRVELLEESPGDVAGLKSAALKVTGDYAFGHLRTETGVHRLVRKSPFDSNNRRHTS
                     FASVFVYPEIDDTIEIEINPADLRIDTFRASGAGGQHVNKTDSAVRITHLPTNIVVAS
                     QNDRSQHRNRAEAFAMLKSKLFELEVRKKNEQKQKLEDTKTDIGWGHQIRSYVLDQSR
                     IKDLRTNVEEGNTQKVLDGDLDEFITASLKQGV"
BASE COUNT          443 a          704 c          647 g          325 t
ORIGIN      
        1 ccggcatcgc cgcgttgctg cgcatcgcgg gtcgcgacgc agcgcgcgca tcgacgtacg
       61 atctcgggtt cgtcgtcggc ccgcgcctga acgccgccgg caggctcgac gacatggcgg
      121 tcggcatcga atgcctgatc accaacgacc atggcacggc ggagcgcatc gcgcgccagc
      181 tcgacgacct gaatcggcag cggcgcgcga tcgagtccga catgcacgaa aacgcgctgg
      241 cgagcctggc cgcaatcaca cccgaagacg gctgcagcct gtgcatgttc gagcccggct
      301 ggcaccaagg cgtgatcggc attgtcgctt cgcggctgaa ggaacgcttc caccggcccg
      361 tcatcgcgtt tgcgcgcggc gacgaaggcg aaatcaaagg ttcgggccgc tcgatcggcg
      421 cgctgcacct gcgcgacgcg ctcgatctgg tcgccaagcg cgaacccgat ctaattctgc
      481 gctttggcgg ccacgcagcc gcagccgggc tcacgctgcg cgaaagcgaa tttgcgcgtt
      541 tccgcgcggc attcgaaatc gtcgccaact cgctgctctc cgcggccgac ctcgagcgcg
      601 tgatcgaaac cgacggcgcg ctgccgctcg agcattgcac gctcgaagcc gcgcgcgtgc
      661 tcgagcacca ggtctggggc cagggattcg ccgcaccgac gttcgacgat gaatttacgg
      721 tcgcgcgcca gcgcgtcgtg ggtcagcgcc atttgaagct gcgcctcgtg aaagacaatc
      781 gtgaggtcga agcgatgctg ttcgcgcatg ccacgccgtt gcccgaccgc atacatgccg
      841 tttaccgcct tgcggtgaac gaatggaacg gcgcgcaaac gctacaactc acgatcgagc
      901 attggcaaaa cgcgtgagca gcgccggata tgcggcgaaa tgcgcggatt tcgcagccac
      961 cgtcctgtat aatcgcgctt ttttcgccgg acactgccgc agaaaccgcc atggaagcag
     1021 aacaactaaa cgccatcgcc cggcggctct cggacttgtc ccagcgcaat gccgagctgc
     1081 ggaggtatct ttgacttcga cgccaagcag ctgcgcctcg ccgaagtcat aaagctcgcg
     1141 gaagacccgg ccgtctggag cgaccccaag cgcgcgcagg atctcggccg cgagcgcaaa
     1201 acgctcgaat cgctcgtcac caccatggcg aagctcgacc aggacctgcg cgacacgagc
     1261 gagctcttca cgatggcgcg cggcgaggac gacgacgcga cgctcaagac catagaggcc
     1321 gatgcgctcg cgctggaaaa aaccgtcgcc gacgtcgaat tccggcgcat gttctcgaat
     1381 ccgatggatc cgaacaactg cttcgtcgac atccaggccg gccagggcgg caccgaagcg
     1441 caggattgga cgcagacgct cgagcgcatg tacatgcgtt attgcgagcg caaaggattt
     1501 cgcgtggagc tgctcgaaga atcgccgggc gacgtcgccg gacttaaaag cgcggcgctg
     1561 aaggtcaccg gcgattatgc gttcggccac ctgcgcaccg aaaccggcgt gcaccgactc
     1621 gtgcgcaaat cgccgttcga ttccaacaac cggcgccaca cgtcgttcgc gagcgtgttc
     1681 gtctatcccg aaatcgacga cacgatagaa atcgagatca accccgccga tctgcgcatc
     1741 gacacgtttc gcgcgtccgg cgccggcggc cagcacgtga acaagaccga ttccgccgtg
     1801 cgcatcacgc acctgccgac gaacatcgtg gtcgcctccc agaacgaccg gtcgcagcac
     1861 aggaaccgcg cggaggcttt tgcgatgctc aagtcgaagc tcttcgagct cgaggttcgc
     1921 aagaagaacg agcagaagca gaagctcgag gacaccaaga ccgacatcgg ctggggccac
     1981 cagatccgct cgtacgtcct cgatcaatcg cgcatcaagg acctgcgcac caatgtcgaa
     2041 gagggcaata cgcagaaggt cctcgacggc gacctcgacg aattcatcac cgcaagcctc
     2101 aaacagggag tctagaaag
//
LOCUS       DATANS010000444         4764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_778295, whole genome shotgun sequence.
ACCESSION   DATANS010000444 DATANS010000000
VERSION     DATANS010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778295"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1729
                     /locus_tag="VHT22_06520"
     CDS             <1..1729
                     /locus_tag="VHT22_06520"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEX3137243.1"
                     /translation="VVFYIAIAILYAHRGRFEARAPVDGLLVFGVPLVGFGLQAALVR
                     DVEYGAAWSALTLASGYGLLFLLLRARAEAGLALLARAFLALAAIFATIAVPLAFDYR
                     VTAALWSVAAAGAFWLGVRQSTPLLRGFALLVEFGAGGVFLWSRAARGDDSLFANAFF
                     VGAMLIAVSGIVTAAVADRAGDKLSAPERGFVPLLLLWGAAWWLAGGAFELKRHLNRI
                     DTPHAALAWVVVSIAAALLVSRAVRWPRLSLVGIALLPAMALVAWSDWQIARTTLENF
                     GALIWPLAWVVQWSALYAADAPQRTGAPSASVPVLTRGQLYAAHAVSAIALTAQIAWE
                     ASEWVDRIALPGTVWLACAAVLPLVAFLFLAYALAGAQRWPLNVHREAYAIGAGGPIA
                     ALAAAWFAIATVVSPGTASPLAYVPLFNPLELTLALTLVALFLWSGRFSGLSDTTRFS
                     CLGVGLFGLLNGAALRTAHHWGAIPWQLNALLASKLLQAGLTLAWTATAVMLMFFATR
                     RALRSLWMTGAGLLALSIGKLFLIDLGALSGLPRVIAFLGVGTLLLLIGYLSPLPPAA
                     DAQKRTDGG"
     gene            complement(1726..2463)
                     /locus_tag="VHT22_06525"
     CDS             complement(1726..2463)
                     /locus_tag="VHT22_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX3137244.1"
                     /translation="MTIPQSLAGAIARARSVAVLTGAGVSAESGVPTFRDAQTGLWAQ
                     FDPQKLATPDAFRRNPKLVWDWYAWRRKLVAKAEPNAGHRALAVLEGRVSDFALITQN
                     VDGLHRRAGSRNVVELHGNIGRVKCSRENTIVEHWTEVGDEVPRCAGCGALLRPDVVW
                     FEETLPPDALQAAEDAARRCQILLVVGTSAEVYPAAALPAYAKSAGATIVEINPNPTP
                     LSDAADYVLRAPAGAVLPALVAAAWPV"
     gene            2572..3735
                     /gene="aroC"
                     /locus_tag="VHT22_06530"
     CDS             2572..3735
                     /gene="aroC"
                     /locus_tag="VHT22_06530"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX3137245.1"
                     /translation="MSGNTFGRLFCVTSFGESHGPAIGCIVDGCPPGLELAAADIQHD
                     LDRRKPGTSRHVTQRRESDTVEILSGVFDGRTTGTPIALLIRNEDARSKDYAAIADTF
                     RPGHADYTYWQKYGIRDHRGGGRQSARETAVRVAAGAVARKWLREHHGLVVRGYLAQI
                     GERTVPFKSWEHVGTNPFFAADDAIGPELEAFMDALRKSGDSCGARITVVASGVPVGW
                     GEPVFDKLDADIAHAMMGINAVKGVEIGAGFASIAQAGTVHSDEMTPQGFASNHAGGI
                     LGGISTGQDIVVSIAVKPTSSIRLDRRSIDKSGAPVTVNTHGRHDPCVGIRAAPIAEA
                     MLLLVLIDHALRHRAQNADVICPTPKIAAAAPADVIARLKSAGAIDNPDPDEA"
     gene            complement(3751..>4764)
                     /gene="xseA"
                     /locus_tag="VHT22_06535"
     CDS             complement(3751..>4764)
                     /gene="xseA"
                     /locus_tag="VHT22_06535"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX3137246.1"
                     /translation="GALYERFARLKARLEAAGWFASERKRALPAFPLALGIVTSPIAA
                     ALRDVLTALAGRFPGLPIILYPAAVQGTGAADEIAAAIAAANAHAQVDVLIVCRGGGS
                     IEDLWAFNEEVLARAVFESRIPVISGVGHETDFTICDFVADVRAATPTTAAALAVPDR
                     AALRRHAQAVFARWQHALARALELRMQRIDLVSRRLIHPAARLERQRADLMALGERLA
                     RAATQAQDARQSRLGQQAAAVARLLKIPPPSRADLERRRERWQRAAAERTAATAQRID
                     ACAQSLRHLNPLGVLDRGYSIVTDDKGAVVQDAGTLAIGDTLNVRFARGQADSKVTRT
                     QSN"
BASE COUNT          665 a         1609 c         1663 g          827 t
ORIGIN      
        1 tgtcgtcttc tatatcgcga ttgcgatcct gtatgcgcat cgcggccgct tcgaggcgcg
       61 cgctccggtc gatggcttgc tggtcttcgg cgtgccgctg gttggtttcg gactccaggc
      121 cgctctggtg cgcgatgtcg agtacggcgc agcctggagc gcactgacgc tggcgagcgg
      181 ttacggcttg ctgttcctgc tgttgcgtgc acgcgccgag gcgggcctgg cgttgctcgc
      241 gcgcgcgttc cttgcgctgg cggcgatctt cgcgacgatc gccgttccgc tggcgttcga
      301 ctatcgcgtc accgccgcgc tgtggtcggt cgcggccgcc ggagcattct ggctcggtgt
      361 gcggcagagc acgccgctgc tgcgcggctt cgcgctgctg gtcgaattcg gcgcgggcgg
      421 agtctttctc tggtcgcgcg cggcgcgcgg cgacgattcg ctgttcgcca acgccttttt
      481 cgtcggggcg atgctgatcg cggtctccgg gatcgttacg gctgcggtgg ccgaccgcgc
      541 cggcgacaag ctgtcggcac cggagcgcgg cttcgttccg ctgctgttgc tgtggggcgc
      601 tgcctggtgg ctcgccggcg gtgcgttcga gctgaagcgg catctgaacc ggatcgacac
      661 gccccatgcg gcgctggcgt gggtcgtggt cagcatcgcg gcggcgctgc tcgtctcgag
      721 ggcggttcgc tggccgcggc tctcgctggt cggcatcgcg ctgctgccgg caatggcgct
      781 ggtggcgtgg agcgattggc agatcgcacg cacgacgctg gagaacttcg gcgctctgat
      841 atggccgctc gcctgggtcg ttcaatggag cgcgttgtac gcggccgatg cgccgcagcg
      901 aaccggcgcg ccgtccgcat cggtgccggt cctcacgcgg ggccagctgt acgccgcgca
      961 cgcggtgtcg gcgatcgcgc tgaccgcgca gatcgcctgg gaagcgagcg agtgggtcga
     1021 ccgcatcgcg ctgcctggga ccgtgtggct tgcctgcgcc gcggtgttgc cgctggtcgc
     1081 cttcctgttc ctggcctatg ccttggcggg cgcacaacgc tggccgctga acgtgcaccg
     1141 cgaggcctac gcgatcggcg ccggcgggcc gatcgccgcg ctggccgcgg cctggttcgc
     1201 gattgccacc gtcgtgtcgc ccggcacggc atcgccgctc gcctatgtgc cgcttttcaa
     1261 tccgctcgaa ctgacgctcg cactgacgct agtcgccctg ttcctgtgga gcggccgttt
     1321 cagcgggctc agcgacacga cgcgattttc ctgcctgggc gtcggcctgt tcggtctgct
     1381 caatggcgcc gcattgcgca cggcgcacca ctggggagcg attccgtggc agctcaacgc
     1441 actgctggcg tcgaagttgc tgcaggcggg attgaccctc gcctggaccg cgactgccgt
     1501 catgctgatg tttttcgcga cgcggcgcgc gctgcgatcg ctgtggatga cgggcgcggg
     1561 cttgctcgcg ctgtcgatcg gcaagctgtt tctgatcgac ctcggagcgc tttctggact
     1621 gccgcgcgtg atcgcgtttc tcggcgtcgg cacgctgttg ctgctgatcg gatacctgtc
     1681 gccgctgccg ccggcggccg acgcgcaaaa gcgcaccgac ggcggctaga ccggccacgc
     1741 ggcggcaacc aacgcaggaa gcacggcgcc tgccggggcg cgcaacacat agtctgcagc
     1801 gtccgacaac ggtgtcggat tcggattgat ctcgacgatc gtcgcgccag cggacttcgc
     1861 gtaggccggc agcgcggctg cgggatagac ctcggccgac gtgcctacga caagcaggat
     1921 ctgacatcgc cttgccgcgt cctcggcggc ctgaagcgca tccggtggca gggtctcctc
     1981 gaaccagacg acgtcgggcc gcagcagcgc gccgcacccg gcgcagcgag gaacctcgtc
     2041 gccgacctcg gtccaatgtt cgacaatcgt attctcgcgg gagcacttga cgcggccgat
     2101 gttgccgtgc agctccacga cgttgcgact gccggcgcgc cggtgcaagc cgtcgacgtt
     2161 ttgcgtgatc agcgcgaaat cggaaacgcg cccttcgagc acggcaagcg cgcgatgacc
     2221 ggcattgggc tccgcctttg cgacgagctt gcgccgccac gcgtaccaat cccacacgag
     2281 cttggggttg cggcggaatg catccggcgt ggcgagcttc tgcggatcga actgcgccca
     2341 caagcccgtc tgggcatcgc gaaacgtcgg cacgccggat tcggcggaga cgccggcgcc
     2401 cgtcaacaca gcgacgctgc gcgccctggc gatcgcgccg gcgagtgatt gcggtatcgt
     2461 catcgatgaa cttcccggta tccgctgcgc gcatcgatgc gggtctgcgt cgtgcgcaag
     2521 tgcatggtgt cgcatgctaa actcgccgca cgtcgacgtc acggatcagc gatgtccggc
     2581 aacaccttcg gcaggctttt ttgcgtcacc tcgttcggcg agtcgcatgg gccggcgatc
     2641 ggctgcattg tcgatggctg tccgccgggg ctggagctgg ctgccgccga catccagcac
     2701 gatctcgacc ggcgcaagcc cggcacgtcg cgacacgtaa cccagcgccg cgaatccgac
     2761 acggtcgaaa tcctgtccgg cgttttcgac ggacgcacga ccggcacgcc gatcgcgctc
     2821 ctgattcgca acgaggacgc gcgcagcaag gattacgcag cgatcgccga tacctttcgc
     2881 cccggtcacg ccgactacac ctattggcag aaatacggca ttcgcgatca tcgcggcggc
     2941 ggccggcagt cggcgcgcga aacggcggtg cgcgttgctg ccggcgcggt cgcgcgcaag
     3001 tggctgcgcg agcatcatgg cctcgtcgtt cgcggttacc tggcgcagat cggcgagcgc
     3061 accgttccgt tcaagtcctg ggaacacgtc ggcaccaatc cgtttttcgc cgccgacgat
     3121 gcgatcggcc cggagctcga agccttcatg gatgccttgc gcaagtcggg cgattcctgc
     3181 ggtgcgcgca tcaccgtcgt ggcttccggc gtgccggtcg gctggggcga gccggtcttc
     3241 gacaagctcg acgcggacat cgcgcacgcg atgatgggta tcaacgcggt caagggcgtg
     3301 gagatcggtg ccggctttgc cagcatcgcg caggcgggga ccgtgcactc ggacgaaatg
     3361 actccgcaag gcttcgcttc gaatcacgcc ggcggcattc tcggcggcat ctcgaccggc
     3421 caggacatcg tcgtctcgat cgccgtcaag ccgacgtcgt cgatacggct cgaccgccgc
     3481 tcgatcgaca aatcgggcgc gccggtgacc gtcaatacgc acggccgcca cgatccctgc
     3541 gtcggcatcc gcgcggcgcc gatcgccgaa gcgatgttgc tgctggtgct gatcgaccat
     3601 gcgctgcgcc accgtgcgca gaacgccgac gtcatctgtc caacgccgaa gatagcggcg
     3661 gctgcgccgg cggacgtgat cgcccggctc aagagcgccg gcgcaatcga caatccggat
     3721 ccggacgagg cctgaagctt ccccgggggc tcagttcgat tgcgtgcgag taaccttgct
     3781 gtcagcttgg ccgcgagcga agcgcacgtt gagcgtgtct ccgatggcga gcgtcccggc
     3841 gtcctgcacg accgcgccct tgtcgtcggt gacgatgctg tagccccgat cgagcacgcc
     3901 cagcgggttc agatgccgca gactttgggc gcaggcgtcg atgcgctgcg cggtcgccgc
     3961 ggtgcgctcg gccgccgcgc gctgccagcg ttcgcggcgc cgctccagat cggcgcgtga
     4021 cggcggcgga atcttgagaa ggcgcgcgac cgccgccgcc tgctgcccca gccggctttg
     4081 ccgagcgtcc tgcgcctgtg tagccgcacg cgcgagccgc tcgcccagag ccatgaggtc
     4141 ggcccgctgt cgctcgaggc gcgctgccgg atggatcagg cggcgcgaca ccagatcgat
     4201 gcgctgcatg cgcagctcca gcgcgcgggc gagggcatgc tgccagcgag cgaagaccgc
     4261 ctgcgcgtgg cggcgcagcg cggcgcggtc cggcactgcc agcgcagcgg ctgtggtcgg
     4321 cgtcgcggcg cgcacgtcgg cgacgaaatc gcatatggtg aagtcggtct cgtgtccgac
     4381 gccgctgatg accgggatgc gcgattcgaa caccgcgcgg gcgaggacct cttcgttgaa
     4441 ggcccacaag tcctcgatcg agccgccgcc gcggcacacg atcaggacat cgacttgggc
     4501 gtgagcattc gcggccgcaa tcgccgcggc gatctcgtcg gcggcgccgg tgccctgtac
     4561 tgccgccggg tacaggatga tcggaagacc tgggaaacgg ccggccagcg cggtcaggac
     4621 gtcgcgcagt gcggcggcta tgggcgatgt gacgataccc agcgccagcg gaaaggcggg
     4681 aagcgctcgc ttacgctctg acgcaaacca gcccgctgct tcaagccgtg ctttcagccg
     4741 ggcaaaacgc tcgtacagcg cacc
//
LOCUS       DATANS010000445         1747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_318439, whole genome shotgun sequence.
ACCESSION   DATANS010000445 DATANS010000000
VERSION     DATANS010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318439"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /locus_tag="VHT22_06540"
     CDS             <1..1358
                     /locus_tag="VHT22_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family metallocarboxypeptidase"
                     /protein_id="HEX3137247.1"
                     /translation="ARPSPGDGGDLAKAGKPTVLIIGQQHGNEPAGGEAALALAAELA
                     DATQAGILEQINVLIVPRANPDGAFHFVRGLKNGGDVNRDHLLEGTPEGRALGKVFVE
                     YRPDVVLDCHEFGVKLRWYEKFQALQAYDALIQYATVSNLPAAITEASERMFRQPLLG
                     ALDGAGLRTSWYYTTAYDVNDRKVSMGGVVPDTGRNIAGLRNAVSFLIETRGVGIGRA
                     HFKRRVQTHLIAMRSVLASTARERDALLSLGRRVRQEVAAAAGKGDLVVTGVAAPGRH
                     VLRMIDPETGADKDVDVEWRSALEIQAKLVRPRPFGYLLPASEATAANRLRDLGVSVL
                     RLNEDADRDVERYRIVAASEAKKDDVRRNDEEGSVDILKLETRTESAMVSFHRGDFYV
                     PLDQPLANVVIAALEPEPQSSYASNRLLAIARPEGASAFLRLYRIAAPLRAAATVWDG
                     K"
     gene            complement(1468..>1747)
                     /locus_tag="VHT22_06545"
     CDS             complement(1468..>1747)
                     /locus_tag="VHT22_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX3137248.1"
                     /translation="SLSPGVNQGTFYVTNDFGNTTTVAAANALIAGLPASGTYVIHLL
                     HNADSQDFVYGVNATLRKVPEPGPLSLVAVALIALLAVRRRGGAKPAA"
BASE COUNT          310 a          539 c          602 g          296 t
ORIGIN      
        1 ttgcgcgtcc ctcgcccggt gacggcggcg atctcgccaa ggccggcaag cctacagtgc
       61 tgatcatcgg ccagcagcac ggcaacgaac cggccggtgg cgaagccgcc cttgcgctgg
      121 cggcggagct ggcggatgcg acgcaggcag gcattctcga gcagatcaac gtgctgatcg
      181 tgcctcgcgc aaatcccgac ggggcgtttc acttcgttcg cggtttgaag aacggcggcg
      241 acgtcaaccg cgaccacctg ctcgagggga cccccgaggg acgcgcgctg ggcaaggtct
      301 tcgtcgaata ccgacccgac gtcgtcctcg attgtcacga gttcggcgtc aagctgcgct
      361 ggtatgaaaa attccaggcc cttcaagcct acgatgcgct gatccagtat gcgacggtat
      421 ccaatcttcc tgccgccatc accgaagcat cggagcgcat gtttcgtcag ccgcttctcg
      481 gcgcgctcga cggcgcggga ttgcgaactt cctggtatta cacgacggct tacgacgtca
      541 acgatcgcaa ggtgtcgatg ggaggggtcg tgcccgatac cggacgcaac attgccggcc
      601 tccggaatgc ggtcagcttc ttgatcgaga cgcgcggcgt aggaatcggc agggcgcatt
      661 tcaagcggcg tgtccagacg cacctgatcg caatgcgttc ggtgctggct tcgactgcgc
      721 gcgagcgcga tgcgctgttg tcgcttggca ggcgggtgcg ccaagaagtg gctgcggccg
      781 cgggcaaggg cgacctggtc gtcaccgggg tcgcggcacc cggtcgtcac gtgcttcgaa
      841 tgatcgatcc ggaaacgggt gccgacaagg atgtcgacgt cgaatggcgc tcggcgctgg
      901 aaatccaggc caagctcgtg cggccgcgcc cgttcggcta tctgctgccg gcgtcggaag
      961 cgacggcagc gaatcggttg cgcgacctgg gagtttcggt gctgcgcttg aacgaagacg
     1021 ccgatcgtga cgtcgagcgc tatcgcatcg tcgcggcgag cgaagcgaag aaagacgacg
     1081 tccgccgcaa cgacgaggag gggagcgtcg acattctgaa gctcgagacc cggaccgaga
     1141 gtgcgatggt ctcgtttcat cgcggcgact tttacgtgcc gctggaccag cctctggcca
     1201 acgtcgtcat cgcggcgctt gagcccgagc cgcaaagcag ttatgcgtcc aatcgcctgc
     1261 tcgcgatcgc caggccggaa ggcgcgtctg cgtttttacg tttgtaccgg atcgccgcgc
     1321 cgctgcgggc ggcggcgacg gtatgggacg ggaagtgagg cgaggacgcg gttgatcgcc
     1381 gccggtccgg gaatcaccgc aagggcaggt cggccccacc cggcaagcgg cgaccgccgg
     1441 ctagccaggt ggaggagggc ggttggatca ggccgcgggt ttcgcgccgc cgcgacgacg
     1501 tacggcaagc agcgcaatca gcgccaccgc gaccagcgac agcggtccgg gttccggcac
     1561 cttacgaagg gttgcgttca cgccgtagac gaaatcctga gagtccgcgt tgtgcagcag
     1621 atgaatcaca tacgtcccgc tagcgggaag gccggcgatc agcgcgttgg ccgccgcgac
     1681 cgtcgtcgta ttaccgaagt cattggtcac gtagaacgtg ccctggttga cgccgggact
     1741 caagctg
//
LOCUS       DATANS010000446         1565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_318448, whole genome shotgun sequence.
ACCESSION   DATANS010000446 DATANS010000000
VERSION     DATANS010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318448"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VHT22_06550"
     CDS             complement(<1..517)
                     /locus_tag="VHT22_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3137249.1"
                     /translation="MFLRWVTAGYLAYLIVPIALLLVGSFGELWLNTLLPTGFTTQWY
                     RNVASDPSFRRAFAASLGVVAIACSACALIGLPLAYAVFHTESRRIRALTRVLYQLPV
                     ALPPLVLAFGFILVFSSDTLPWLGSIWLLAAGHVVLTLPYFLQAVVSDMQRLGLRALE
                     DAAESLGGSAWQ"
     gene            630..>1565
                     /locus_tag="VHT22_06555"
     CDS             630..>1565
                     /locus_tag="VHT22_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEX3137250.1"
                     /translation="MTRIAVIDDWQKIARRSADWSVLTARADVQFFETPFADENDAAR
                     RLAEFDIVLAIRERTPFPASLVARLPKLKMFGQTGARAALIDSAAMIAQGITVCYTGG
                     GPSGTSTAELALGLMLAAARRIPAADAGVRDGRFQSGTEAGQILEGKTVGLIGLGRIG
                     SRMAAYCKALGMTVLAWSQNLSAETATAAGAVRVGKEELLSAADVVSLHLQLSDRTRG
                     ILGAADLARMRPGAILVNTARAQLVDEAALMAAVSSGRLFAALDVFYREPLPAGHPLA
                     RAANVVLTPHLGYSVVEVYREYYQHAVENALAFLDG"
BASE COUNT          281 a          485 c          556 g          243 t
ORIGIN      
        1 gctgccatgc gctgccaccc agggactcgg cggcgtcttc gagcgctcgc aagcccaggc
       61 gctgcatgtc ggacacaacc gcctgcagga agtagggcaa cgtgaggacg acgtggccgg
      121 ccgcgagcag ccagatgctt cccagccagg gcagcgtatc cgacgaaaag accaggatga
      181 agccgaaggc aagcaccagc ggcgggagcg ctaccggaag ctggtagagc acgcgggtga
      241 gcgcacgtat gcgacggctt tccgtgtgga acactgcgta cgcgaggggc aatccgatca
      301 gcgcgcatgc ggaacatgcg atggccacga cgccgaggct cgccgcaaac gcgcggcgaa
      361 aactggggtc ggaggcgacg tttcggtacc actgcgtcgt aaagcccgtc ggcagcagcg
      421 tgttgagcca tagctcgccg aacgagccga ccagcagcag cgcgatcggc acgatcaggt
      481 aagcgaggta accggcggtg acccagcgca ggaacatcgg tcgcatgctc gggaaagggg
      541 gggcggcctg gttcgccgtc gagacgaatg atagacggga tttgctacca tcgcgaacag
      601 cgagtcaggc gaaagggagc ggcagatcgg tgaccaggat tgcggtaatc gacgattggc
      661 agaagattgc gcggcgcagc gctgactggt cggtacttac cgcacgtgcc gacgtgcaat
      721 ttttcgaaac gccgttcgcc gatgagaacg acgcggctcg gcggctcgcc gagttcgaca
      781 tcgtgcttgc catccgggag cgaacgccgt ttccggcctc gcttgtcgcg cgactgccga
      841 agctgaagat gttcgggcag actggcgcgc gggccgcgct gatcgatagc gcggcgatga
      901 tcgcacaggg catcaccgtc tgctacaccg ggggcgggcc gtcgggaact tcgacggcgg
      961 agctcgcact cggcctgatg ctcgccgctg cccgacggat tcccgccgcc gatgccggtg
     1021 tgcgtgacgg ccgctttcag tcggggaccg aagcgggcca gatactcgag ggcaagacgg
     1081 tcggtctgat cggcttgggc cggatcggct cgcgcatggc ggcgtattgc aaagcgctgg
     1141 ggatgactgt cctcgcctgg agccagaatc tgagcgccga gaccgcgact gcggccggcg
     1201 cggttcgggt cggcaaggag gaactgctgt ctgccgccga cgtcgtcagc ctgcatctgc
     1261 agctttcgga ccgcacgcgg gggatccttg gcgcggcgga cctcgctcgg atgcgaccgg
     1321 gtgcaatcct ggtcaatacc gcgcgcgctc aactggtcga cgaagccgcg ctgatggccg
     1381 ccgtctcgtc agggcggctg ttcgccgcgc tcgacgtttt ctaccgcgag ccgctgcctg
     1441 ccggtcatcc gctcgcgcgc gctgccaacg tcgtgctgac gccgcacctg ggatacagtg
     1501 tggtcgaggt gtaccgcgag tattaccagc acgccgtcga aaacgcgctg gcgtttctcg
     1561 acggc
//
LOCUS       DATANS010000447         5170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_22135, whole genome shotgun sequence.
ACCESSION   DATANS010000447 DATANS010000000
VERSION     DATANS010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22135"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..1732
                     /gene="rpsA"
                     /locus_tag="VHT22_06560"
     CDS             11..1732
                     /gene="rpsA"
                     /locus_tag="VHT22_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX3137251.1"
                     /translation="MTTANTNPAAPAQENFATLFEESLARQGMRQGELITAEVTRVDY
                     NIVVVNAGLKSESFIPIEEFKNDKGEVEVKAGDFVTVAIESLEDGYGETRLSRDKAKR
                     LKAWHDLEAAMEQGTIVSGVVSGKVKGGLTVMVNGIRAFLPGSLVDIRPVKDTSPYEF
                     KTLEFKVIKLDRKRNNVVVSRRAVLEASLGEEREKLLSTLQEGATVKGIVKNITDYGA
                     FVDLGGIDGLLHITDLAWRRVKHPSEVLAVGDEVTAKVLKFDQEKNRVSLGMKQLGED
                     PWVGLSRRYPPGTRLFGKVTNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNIHPTKVVQ
                     LGDEVEVMILEIDEERRRISLGMKQCMPNPWEEFSINHKKGDKVKGLIKSITDFGVFI
                     GLPGGIDGLVHLSDLSWSATGEAAVRDYKKGQEVEAVVLAIDVERERISLGIKQLDGD
                     PFSNYVATHDKGSLVSGTVKSLDGKGAVIALDGDVEGYLRASEVARDRVEDIRSHLKE
                     GDSVNAMIINIDRKNRSINLSIKAKDSADESEALQRMTAENSGTTAGTTNLGALLKAK
                     LDNKNQG"
     gene            1745..2041
                     /locus_tag="VHT22_06565"
     CDS             1745..2041
                     /locus_tag="VHT22_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEX3137252.1"
                     /translation="MTKSELIDRLAAQFPQLVAKDAELAVKMILDAMAESLAKGERIE
                     IRGFGSFGLNYRPPRTGRNPKSGERVQVPEKHVPHFKAGKELRERVDFKEMKKG"
     gene            2081..2353
                     /locus_tag="VHT22_06570"
     CDS             2081..2353
                     /locus_tag="VHT22_06570"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HEX3137253.1"
                     /translation="MRFVRWLVGLVVFAGLLLLALQNAEPVTLHVYDGVGWRTPLIFL
                     LLIVFAAGVAAGLLAGSIRSVRLKRQLNRLRHQQRRAPVTRPRDGG"
     gene            2369..3547
                     /gene="lapB"
                     /locus_tag="VHT22_06575"
     CDS             2369..3547
                     /gene="lapB"
                     /locus_tag="VHT22_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEX3137254.1"
                     /translation="MEIEIWWLLALTLLFFALGWIAARIDIKHLLRESRALPLSYFRG
                     LNFLLNEQPDKAIESFIEVVKVDPQTIDLHFALGNLFRRQGEIDRAIRMHQNLLDRPD
                     LPPDKRETAVFELAQDFHRAGLLDRAEDLFTKLDGSTFELQSLGSLLQIYEQEKDWPK
                     AIGATRRMETLAKHPYFKEIAHYHCEMAQGSLLRSRGDEARRFIDQALAEYKLCTRAT
                     MLLGDLEAQQGNDAAAITAWQRIESQNPAFLGLVADRLADAYRRTGDVAQGIRVLRNY
                     EDQYPSLDLITTLFNQILAHDGPEAGTQLIKDELSRNPTLLGLDKLLEAQLMEAPAER
                     RHDLELVKTLVSQHIKRLGMYRCEQCGFRAKQYYWRCPGCGKWETYSPRRTETPDGFA
                     "
     gene            3634..4356
                     /gene="pyrF"
                     /locus_tag="VHT22_06580"
     CDS             3634..4356
                     /gene="pyrF"
                     /locus_tag="VHT22_06580"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX3137255.1"
                     /translation="MNAADPRVIVALDFPDMLSAFGLAARLDPAVCAVKVGKELFTVA
                     EAGLVRELVRRGFRVFLDLKFHDIPNTVAQACAAATRLGVWMIDVHAAGGAAMLAAAR
                     DAVARTAAEERRPRPLLIAITVLTSLDASDLAAIGVGGTPEAQALRLARLAKANGLDG
                     VVCSAQEAAMLRSACGPAFKLVTPGIRPEGAAAHDQARVMTPEAAIAAGADYLVIGRA
                     ITAAADPVRALREINASLGIAA"
     gene            4353..>5170
                     /locus_tag="VHT22_06585"
     CDS             4353..>5170
                     /locus_tag="VHT22_06585"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX3137256.1"
                     /translation="MKITMIGTGYVGLVSGTCLAEVGNDVLCLDVDARKIDMLKAGGV
                     PIHEPGLQEMIHRNVEAGRLRFTTDADAAVAHGSLQFIAVGTPPDEDGAADMQHVLAA
                     AAAIGRRMTDYKVIVDKSTVPVGTADRVRETIAAELSARGAKIPFAVVSNPEFLKEGA
                     AVEDFMRPDRIVIGADDDRAIASLRAVYAPFQRNRERLLVMDVRSAELTKYAANAMLA
                     TRISFMNEMANLADELGADIEHVRQGIGSDPRIGYHFLYPGAGYGGSCFPKDVKA"
BASE COUNT          999 a         1641 c         1691 g          839 t
ORIGIN      
        1 accacgatac atgacaaccg caaacaccaa ccccgctgct ccggcccagg agaacttcgc
       61 caccttgttc gaggagtcgc tcgcaaggca gggaatgcgg cagggcgaac tgatcacggc
      121 cgaagtgacg cgcgtcgatt acaacatcgt cgtcgtcaac gcgggcctca agtccgagag
      181 cttcatcccg atcgaggaat tcaagaacga caagggcgaa gtggaagtca aggccggcga
      241 tttcgtcacc gtcgcgatcg agagcctcga agacggctac ggcgaaaccc gcctgtcgcg
      301 cgacaaagcc aagcggctga aggcctggca cgatctcgaa gccgcgatgg agcaggggac
      361 tatcgtttcc ggcgtcgtca gcggcaaggt caagggcggc ctgacggtca tggtcaacgg
      421 catacgcgcg ttcctgccgg ggtcgctggt cgacatccgg ccggtcaaag acacttcgcc
      481 gtacgaattc aagacgctcg aattcaaggt catcaagctc gaccgcaagc gcaacaacgt
      541 cgtcgtctcg cggcgcgccg tgctcgaagc gagcctcggc gaggagcgcg agaagctcct
      601 gtcgacgctg caggaaggcg cgacggtcaa gggcatcgtc aagaacatca ccgactacgg
      661 tgcgttcgtc gatctgggcg gcatcgacgg cctgctgcac atcaccgacc tggcgtggcg
      721 ccgggtcaag catccgtcgg aggtgctcgc cgtgggcgac gaggtcaccg ccaaggtgct
      781 caagttcgac caggagaaga atcgcgtttc actgggcatg aagcagctcg gcgaggaccc
      841 atgggttggc ctgtcgcgcc gctacccgcc cggcacacgc ctgttcggca aggtcaccaa
      901 cctgaccgac tacggcgcat tcgtcgagat cgagcagggc atcgaaggcc tggtccacgt
      961 ctccgaaatg gactggacga acaagaacat ccatccgacc aaggtcgtgc agctgggcga
     1021 cgaggtcgag gtgatgatcc tggaaatcga cgaggagcgc cgccggatct cgctcggcat
     1081 gaagcagtgc atgccgaatc cgtgggaaga gttctcgatc aaccacaaga agggcgacaa
     1141 ggtcaaagga ctgatcaagt cgatcaccga cttcggcgtt ttcatcggac tgccgggcgg
     1201 catcgacggc ctggtgcacc tttccgacct gtcgtggtcg gcgaccggtg aggctgcggt
     1261 gcgcgactac aagaaggggc aggaagtcga ggcagtcgtg ctggcgatcg acgtcgagcg
     1321 cgagcgtatc tcgctcggca tcaagcagct cgacggcgac ccgttctcga actacgtcgc
     1381 gacgcacgac aagggcagcc tcgtcagtgg aacggtgaag tcgctcgacg gcaaaggagc
     1441 ggtcatcgcc ctcgatggcg acgtcgaagg ctatctgcgc gcatcggaag tcgcgcgcga
     1501 ccgggtcgag gatatccgct cgcacctgaa ggaaggcgac tcggtcaacg cgatgatcat
     1561 caacatcgac cgcaagaatc ggtcgatcaa tctgtcgatc aaggcgaagg acagcgccga
     1621 cgaatcggaa gcgctgcagc gcatgaccgc agagaattcc ggcaccaccg ccggaacgac
     1681 caacctgggg gcgctgctca aggccaagct cgacaacaag aaccaagggt aggcggctca
     1741 cgtgatgacc aaatcggagc tgatcgaccg cttggcggcg cagttcccgc aattggtggc
     1801 gaaggacgcc gagcttgcgg tgaagatgat cctcgacgcg atggccgaaa gtctggccaa
     1861 gggcgagcga atcgaaatcc gcgggttcgg cagcttcggc ctcaactatc ggccgccgcg
     1921 caccggcagg aaccccaagt cgggcgagcg tgtccaggtg ccggagaagc acgttccgca
     1981 cttcaaggcc ggcaaggaat tgcgcgaacg cgtcgacttc aaggaaatga aaaagggttg
     2041 atgatgaccc ggggtgaaat attctcattg gactcgcagc atgagattcg tccgctggct
     2101 cgtcgggctc gtcgtcttcg caggcctgct gctgcttgcg ctgcagaacg cggaaccggt
     2161 cacgctccat gtatatgacg gcgtcggttg gcggacgccg ctgatcttcc tgcttctgat
     2221 cgtgttcgcc gccggggtcg cggcaggcct gctggccggt tcgatccgct ctgtgcgtct
     2281 gaagcggcaa ttgaaccgcc tgcgccacca gcagcggcgc gcgccggtga cgcgtccgcg
     2341 cgacggaggc tgaagcgaag tgccgaccat tgaaatcgag atctggtggc tgctggcact
     2401 gacgctgctg tttttcgcgc tgggctggat cgccgcccgc atcgacatca agcatctgct
     2461 gcgcgaatcg cgcgcgctgc ccttgtcgta cttccgcggc ctgaacttcc tgttgaacga
     2521 gcagcccgac aaggcgatcg agtcgttcat cgaagtcgtc aaggtcgatc cgcagacgat
     2581 cgatctgcat ttcgcgctcg gtaacctgtt ccgccgccag ggtgagatcg atcgcgcgat
     2641 ccggatgcat cagaacctgc tcgaccggcc cgacctgccg ccggacaagc gcgagaccgc
     2701 ggtcttcgag ctcgcccagg actttcatcg cgccggcctg ctcgaccggg ctgaggatct
     2761 gttcaccaag ctcgacggca gcacgttcga gctgcagtcg ctgggctcgc tgctgcagat
     2821 ctatgagcag gaaaaggact ggccgaaggc gatcggcgcg acccgccgca tggaaacgtt
     2881 ggccaagcat ccgtacttca aggaaatcgc ccactaccac tgcgagatgg cgcaaggctc
     2941 gttgctgcgc tcgcgaggcg acgaagcccg gcgcttcatc gaccaggcgc tggccgaata
     3001 caaactgtgc acgcgggcga cgatgctgct gggcgacctc gaggcgcaac agggcaacga
     3061 tgcggcggcg atcaccgcat ggcagcgcat cgagtcgcag aacccggcgt ttctcggcct
     3121 ggtggccgac cggctcgccg acgcataccg caggaccggc gacgtcgcgc agggcattcg
     3181 cgtgctgcgc aactacgagg accagtatcc ttcgctcgat ctgatcacga cgctgttcaa
     3241 ccagatcctg gcccacgacg ggccggaagc gggaacgcaa ctgatcaagg atgagctctc
     3301 gcgcaatccg accctgctgg ggctcgacaa gctgctcgaa gcgcaactga tggaagcgcc
     3361 ggcggagcgc cgtcacgacc tggagctggt caagacgctg gtgagccagc acatcaagcg
     3421 gctgggcatg tatcggtgcg agcaatgcgg ctttcgcgcc aagcagtact attggcgttg
     3481 cccgggctgc ggcaaatggg aaacgtactc gccgcgacgg accgagacgc cggatggatt
     3541 cgcgtgaagc ggctccgcgc tgaaccgggg gcgccgcggg cggctcctga cgcgcagagt
     3601 ttcagctagg atgcgatcga ggacaccgaa ccggtgaacg ccgctgatcc ccgcgttatc
     3661 gtcgcgctgg actttcccga catgctgagc gccttcggcc tcgctgccag gctcgacccg
     3721 gcggtgtgcg cggtcaaagt cggcaaggag ctgttcaccg tcgccgaagc cggcctggtg
     3781 cgcgagctcg tccgccgcgg ctttcgcgtg ttcctggatc tcaagtttca cgacatcccg
     3841 aatacggtgg cgcaggcctg tgcggcggcg acgcggctcg gcgtctggat gatcgacgtt
     3901 cacgccgctg gcggtgcagc gatgctcgcc gcagcgcgcg acgccgtcgc gcggacggcg
     3961 gccgaagaac gtcgaccgcg cccgcttttg attgcgatca ccgtgctgac cagcctcgat
     4021 gcgtccgatc tggccgcaat cggcgtcggc ggtacacccg aagcgcaggc cttgcggctc
     4081 gcccgactcg ccaaggcgaa cggcctcgac ggcgtcgtct gctcggcgca ggaggctgcg
     4141 atgttgcgct ccgcatgcgg cccggcattc aagctggtca cccccggcat ccgcccggag
     4201 ggcgcagcgg cgcacgacca ggcgcgggtg atgacaccgg aagccgctat cgcagcgggc
     4261 gccgattacc tggtcatagg ccgcgcgatc accgcggcgg cggacccggt gcgcgccttg
     4321 cgcgagatca acgcctcgct gggcatcgcc gcttgaagat cacgatgatc ggtaccggat
     4381 acgtgggact cgtctccggg acgtgcctcg cggaagtcgg caacgacgtg ctctgcctcg
     4441 acgtcgatgc acgcaagatc gacatgctga aggccggcgg cgtgcctatc cacgagccgg
     4501 gactgcagga aatgatccat cgcaacgtcg aagccgggcg cttgcgcttc acgaccgatg
     4561 ccgatgccgc ggtggcgcac ggcagtcttc agttcattgc cgtcggcacg ccgcccgacg
     4621 aggacggcgc cgccgacatg cagcatgtcc ttgccgccgc ggccgcgatc ggccggcgca
     4681 tgaccgacta caaggtcatc gtcgacaagt cgaccgttcc cgtcggcacc gccgaccggg
     4741 ttcgagaaac gatcgctgcc gaactgtcag ctcggggcgc gaaaattccg tttgccgtcg
     4801 tgtcgaatcc ggaattcctg aaggaaggcg ccgcggtcga agacttcatg cggcccgacc
     4861 gcatcgtcat cggcgccgac gacgatcgtg cgatcgcgtc gctgcgtgct gtgtacgcgc
     4921 cattccagcg caaccgggag cggctgctgg tcatggacgt tcgctcggcc gagctcacca
     4981 aatacgcggc caatgcgatg ctcgcgaccc gcatctcgtt catgaatgaa atggcgaatc
     5041 tcgccgacga gctaggcgcc gacatcgagc acgtccgcca gggtatcgga tcggatccgc
     5101 gcatcggcta tcatttcctc tatccgggcg ccggctatgg cggatcatgt ttccccaagg
     5161 acgtgaaagc
//
LOCUS       DATANS010000448         5834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_778410, whole genome shotgun sequence.
ACCESSION   DATANS010000448 DATANS010000000
VERSION     DATANS010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5834
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778410"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..791)
                     /locus_tag="VHT22_06590"
     CDS             complement(18..791)
                     /locus_tag="VHT22_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3137257.1"
                     /translation="MGIIVGMEASSTAPASVSTGTMAAWFATPLGRHLLAREQTYFDT
                     TLADIFGYHALQIGLPETPLLRASRIATHCTVDLEEPAQVLADPHWLPFRENSFDLVV
                     LPHALEFTDEPHQLLREVYRVIRPEGQLVVSGFNPFSLFGAKRYFGREQTMPWTGNFI
                     SLYRVKDWLALLGFEVSGGRLDAYVPPVALEKWLRRFEFFERAGDRWWPIAGGVYYLR
                     ATKRVAGMRVITPAWTRRVRKKALAPVARQAKEYVDAID"
     gene            772..1551
                     /gene="gloB"
                     /locus_tag="VHT22_06595"
     CDS             772..1551
                     /gene="gloB"
                     /locus_tag="VHT22_06595"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEX3137258.1"
                     /translation="MPTIIPIPAFADNYIWLLREGRAAAVVDAGDAAPVIDYLDREQV
                     ELTAIICTHHHGDHVGGNRALLERWQVPVYGPARETIPGRTHAMRENDEVVVAGIGLA
                     LSVLDIPGHTAGHIAYVARSDAAPLVFCGDTLFAVGCGRLFEGTPTDMVASLDKLAAL
                     PANTLVYCGHEYTLSNLRFALAVEPGNAALREREITAKGQRARGEPTLPSTIGDERAT
                     NPFLRTGETTIRAAASAHADRELADRIGVFAEIRAWKNAFR"
     gene            1834..3225
                     /locus_tag="VHT22_06600"
     CDS             1834..3225
                     /locus_tag="VHT22_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX3137259.1"
                     /translation="MLARAVVAPSVDEPAVSIEDPTAITVPRPPANGNLWERIRAGFA
                     MPDLDTPQVKKAADWYAANPEYVGRMVERSRRYLYYIVGQVEKRGMPMEIALLPMIES
                     AFNPMAYSRSRASGIWQFMPSTGKIYGMQQTWWFDERRDIVAATDGALDYLEKLYQEF
                     GDWQLALAAYNWGEGSVRRAIAANAKRRLPTDYLSLKMPAETRGYVPKLQAIENIVRD
                     PKAYGLKFADIPDAPYFSVVRTSRKMDVKIAAELADMPLEEFLSLNPQHNRPVIAGAD
                     EASILLPYDKAGLFAAKLELSDQPMVTWQAYRLKTGETLQQVATRFGLPLETLRAVNG
                     IGPRAQVPVNHTLLVPSQLPSSATAATLQSAVFTTVPEGRTFYHRVRNGETLYGIAAR
                     YHVSAQELKAWNPDVKGKLVRGQRLRVIGDAAPAGGKSKHGGKSHKSATATAPSAGKP
                     PGSASSAALAAPR"
     gene            complement(3332..3682)
                     /locus_tag="VHT22_06605"
     CDS             complement(3332..3682)
                     /locus_tag="VHT22_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137260.1"
                     /translation="MMTMSTPSAPAAAPKKKAKRKAAPRKKAAKKAAPKKKAAKKKKA
                     APKKKAAKRKPAKKKAAKRKATKKKAAPKRKAAKRKPAKKKAAKRKVAKKKAAPKKKA
                     AKRKPAKRKAAKKK"
     gene            complement(3927..5171)
                     /gene="lysA"
                     /locus_tag="VHT22_06610"
     CDS             complement(3927..5171)
                     /gene="lysA"
                     /locus_tag="VHT22_06610"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX3137261.1"
                     /translation="MTAFDYRDGELAAEGVALAEIAAKHGTPCFVYSRAAIEGAFATF
                     DRAFAGVPHLVCYAMKANSNLAIINLLARLGSGFDIVSGGELARAIAAGADPARIVFS
                     GVGKTDAEMASALELGILCFNLESASELEHLNRVAGSLGRRAPISFRVNPDVDPKTHR
                     YISTGLKGDKFGVAFHDALPLYRRAAGLPHVAVRGIDMHIGSQITTLEPHCEAAAKAF
                     DLVDRLGAEGIALEHIDLGGGIGIRYRDEETIAPADYAAAIRALLGKRPLKLLFEPGR
                     MLVGNAGALLTRVLYLKPGADRNFAIVDAAMNDLLRPALYDAWHEVRAVAPRSGPPTV
                     WQIVGPVCESADFLAHDRVLDLAEGDLLAILSAGAYAMAMSSNYNSRPRAAELVVDGK
                     TAHLVRPREVAEELFAREILLP"
     gene            5501..>5834
                     /gene="cyaY"
                     /locus_tag="VHT22_06615"
     CDS             5501..>5834
                     /gene="cyaY"
                     /locus_tag="VHT22_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEX3137262.1"
                     /translation="MFSASADAVLAAVGAALDAAGDASDADLDWSLNDGVLTIDCGRD
                     GKLIVNRHVPNREIWVAAKSGGFHFRAAGGAWRDSRGGEELGAALTRLLRDQAGTTID
                     LTALPPPPE"
BASE COUNT          981 a         1907 c         1903 g         1043 t
ORIGIN      
        1 cgccggcgcg cgcatcctca atcgatcgca tctacatact ctttcgcttg ccgcgcgacc
       61 ggtgcgagcg ccttcttgcg cacgcggcgt gtccacgccg gcgtgatcac ccgcatgccc
      121 gccacccgct tggttgcacg caggtaatac acgcctccgg caatcggcca ccagcgatcg
      181 ccggcccgtt cgaagaactc gaagcgccga agccactttt ccagggcgac cggcggcaca
      241 taggcatcga gcctgccccc gctgacctcg aatccgagca gtgcgagcca gtccttcacc
      301 cgatacagcg atatgaagtt gccggtccag ggcatcgtct gctcgcggcc gaaataacgc
      361 ttggcaccga acaggctgaa cggattgaat ccgctgacga ccagctgacc ctcgggacgg
      421 atcacgcgat acacttcgcg cagcagctga tgcggctcgt cggtgaattc cagcgcgtgc
      481 ggaagcacga ccagatcgaa gctattttcc cgaaacggaa gccaatgcgg gtcggccaga
      541 acctgcgccg gctcctcgag gtcgacggtg cagtgggtcg cgatgcgcga ggcccgcagc
      601 agcggagttt ccggcagtcc gatctgcaac gcgtgatagc cgaagatgtc ggccagcgtc
      661 gtgtcgaaat acgtctgctc gcgagccagc aggtgtcggc ccagcggagt ggcgaaccac
      721 gccgccatcg tgccggttga cacgctggcc ggtgctgtcg aagatgcttc catgccgacg
      781 attatcccca tccccgcgtt cgccgacaac tacatatggt tgctgcgcga aggccgagcc
      841 gcagccgtcg tcgatgccgg cgacgctgcg ccggtgatcg actatctcga ccgcgagcag
      901 gtcgagctca ctgcgatcat ttgcacgcat catcacggcg atcatgtcgg tggcaaccgc
      961 gcgctgctcg agcgttggca agtccccgtc tacggaccgg cgcgcgagac gatacccggg
     1021 cgcacgcatg cgatgcgcga gaacgacgag gtcgtcgttg cgggcatcgg gctcgcgctg
     1081 tcggttctcg atattcccgg ccacaccgcc ggccatatcg cttacgtcgc ccggtccgat
     1141 gccgcaccgc tggtgttttg cggcgacacg ttgtttgccg tcggctgtgg ccgactgttc
     1201 gaggggacgc cgaccgacat ggtggcctcc ctcgacaagc ttgccgcgct gccggccaat
     1261 acgcttgtct actgcggaca cgaatacacc ctttccaacc ttcgcttcgc tttggcggtc
     1321 gaaccgggga atgctgcact gcgcgaacgc gaaatcacgg ccaaaggaca gcgcgcgcgg
     1381 ggcgagccga cgctgccgtc gacgatcggc gacgaacgcg cgaccaaccc gtttttgcgc
     1441 actggagaaa cgacgattcg ggccgcggcc tcggcgcatg ccgaccgcga gctcgccgac
     1501 cgaatcggcg tatttgccga aatacgcgcg tggaagaatg cgttccggta gcgggccggg
     1561 gacttcgcgc gcttgacggt agctgcgcct gccccctacc atcgcggcac attgcgcgct
     1621 atcgaactgc ctacgcctgc cgtcgccaga gcagccgcca tcgccgcggt ggcattcgca
     1681 ttggcgtcct gtgccacgat gccaggctcg gcgcccggcg acgcgacggc ctccgttgca
     1741 tcctccgcag tggagccagc cgaatcctcg gttgacctcg gtcccgaacc ggccgcaacg
     1801 tggcgaccaa cgccggagat ggaggacgcg acgatgcttg cgcgcgcggt agtggcgccg
     1861 agcgtcgacg agccggcggt cagcatcgag gatccgaccg cgatcaccgt accccgaccg
     1921 ccagccaacg gcaacctgtg ggagcgcatc cgcgccgggt ttgcgatgcc cgatctggat
     1981 acgccgcaag taaagaaggc ggccgactgg tatgcggcca atccggagta cgtcgggcgg
     2041 atggtcgagc gcagccggcg ctacctgtac tacatcgtcg ggcaggtaga aaagcgcggg
     2101 atgccgatgg agatcgcgct gctgccgatg atcgaaagcg cattcaatcc gatggcgtat
     2161 tcgagaagcc gcgcctcggg tatctggcag ttcatgccgt cgaccggcaa gatctatggc
     2221 atgcagcaga cctggtggtt cgacgagcgg cgcgacatcg tcgccgcgac cgatggcgcg
     2281 ctcgattatc tggaaaagct ttaccaggaa ttcggcgact ggcagctcgc cctcgccgcg
     2341 tacaactggg gcgagggatc ggtgcggcgg gcgatagccg ccaacgcgaa gcgcaggctg
     2401 ccgaccgatt atctgagcct caagatgccg gcggagacgc gcggctatgt gcccaagctg
     2461 caggcgatag aaaacatcgt ccgggacccg aaggcctatg ggctcaagtt cgccgacatt
     2521 cccgacgcgc cgtacttcag cgtcgtacgc accagccgca agatggacgt caagatcgcg
     2581 gccgagcttg cggacatgcc gctggaggaa ttcctttcgc tcaatccgca gcataatcgc
     2641 ccggtgatcg ccggcgccga cgaggcatcg atcctgctgc cttacgacaa ggccggcttg
     2701 ttcgccgcca agctggagct ctcggaccag ccgatggtca cttggcaggc gtaccggctc
     2761 aagacggggg aaacgctgca gcaggtcgcc acacggttcg gcctgccgct ggagacgctg
     2821 cgcgccgtca atggcatcgg tccacgcgcg caggtgccgg tcaatcacac gctgctcgtg
     2881 ccgtcgcagc tgccctccag cgcgaccgcg gcgaccttgc agagcgcagt cttcacgacg
     2941 gtcccggaag ggcggacgtt ctatcatcgc gtccgcaacg gcgagacgct ctacggcatc
     3001 gccgcgcgct atcacgtcag tgcgcaggag ctcaaagcct ggaatcccga cgtcaagggc
     3061 aagctggtgc gcgggcagcg actgcgcgtg atcggcgatg ccgcgcccgc cggaggcaag
     3121 tccaagcacg ggggcaagag ccacaagagc gccactgcca cggcgcctag cgccggaaag
     3181 ccgccaggca gcgccagcag cgcggcattg gcggctcccc gctagcgccg ctgtcgggcg
     3241 atttcgtcgc ccccaaacaa aacaggcgac acttgcgcgt cgcctgtttt gaggtccatc
     3301 gcagctgcga cggacggatt gccgcgtaag cttatttctt cttggcggcc tttctcttgg
     3361 ccggtttgcg cttggcggcc ttcttcttcg gggcagcctt ctttttcgca accttgcgct
     3421 tcgctgcctt tttcttggcg ggcttgcgct ttgcggcctt gcgcttcggg gcggccttct
     3481 tcttcgttgc cttgcgcttc gctgccttct tcttggcggg cttgcgcttg gcggccttct
     3541 tcttcggggc agccttcttc ttctttgcag ccttcttctt cggggcagcc ttcttcgccg
     3601 ccttcttcct cggcgccgct tttcgcttcg ccttcttctt cggcgccgcg gccggagccg
     3661 acggtgtcga catcgtcatc atgtcagcca tcatttgcat ttctcctgtc tgagttgcta
     3721 aaactgttat gagtgcttct cttcgtcgca ggtttcgccg gaatcggccg tcgagattta
     3781 ctgcgcctct tgacaaaccc attgacacct gctgcctgaa ttctagccaa tttttttcga
     3841 aatacaagat ttaattgctg ttttaggtga cgctgtacgt cagtgttgcg cttttgcaca
     3901 ggcgatttgc gggctcgcgc aagtgatcaa ggaagcaaaa tttcgcgcgc gaatagttcc
     3961 tctgcgacct cgcgcggacg gacgagatgc gccgtttttc cgtcgacaac cagctcggcg
     4021 gcgcgcggcc gcgagttgta attcgagctc attgccatcg cgtaggcgcc ggccgaaagg
     4081 atggcgagca ggtcgccttc cgcaaggtcg agcacacggt cgtgcgccag gaaatccgcg
     4141 ctctcgcata ccggtccgac gatctgccag accgtgggcg gtccggagcg tggcgcgacc
     4201 gcgcgcacct cgtgccaggc gtcgtaaagc gcggggcgca gcaggtcgtt catcgccgcg
     4261 tcgacgattg cgaaattgcg atccgcgccc ggcttcagat agagcacgcg ggtaagcagc
     4321 gcaccggcat tgccgaccag catccggccg ggctcgaaca gcagtttcag cgggcgcttt
     4381 cccagcaggg cgcggatagc tgcagcgtaa tcggccggcg ctatcgtctc ctcgtcgcgg
     4441 tagcggatgc cgattccgcc acccaggtcg atgtgctcga gcgcgatacc ctcggcgccc
     4501 agacggtcga ccagatcgaa cgccttggcg gccgcctcgc aatgcggttc cagcgtcgtg
     4561 atctgcgagc cgatgtgcat gtcgatgccg cgcacggcga cgtggggcag gcctgcggcg
     4621 cgtcggtaaa gtggcagcgc gtcgtgaaat gcaacgccga acttgtcccc cttgaggccg
     4681 gtcgagatgt accgatgcgt cttcggatcg acatccggat tgacccggaa gcttattggc
     4741 gcccgcctgc ccagggagcc ggcgacccgg ttcaaatgct cgagctcgct cgccgattcg
     4801 aggttgaagc agagtatccc gagctctagc gcgctggcca tttcggcgtc ggttttgccc
     4861 acgccggaga aaacgatccg cgcgggatcc gcgccggcag cgatcgcgcg cgcaagctcg
     4921 ccgcccgaca cgatgtcgaa gccgctgccc aggcgcgcga gcaggttgat gatcgccaga
     4981 ttcgagttgg ctttcatcgc gtagcagacc aggtggggca cgccggcgaa tgcgcgatcg
     5041 aacgtcgcaa aggcaccttc gatcgcggcg cgggaataga cgaagcaggg agtgccgtgc
     5101 tttgcggcga tttcagcgag cgcaacgccc tcggcggcga gctcgccgtc gcgatagtcg
     5161 aacgcggtca ctgcttgccg gcgccggatg atccgggcgc ggtcggaggc tcggccggcg
     5221 gcggggaaga cggagtggac gacggcggcg gcgtggtcag cggagcgccc ggttcagtcg
     5281 tcggagccgc ggcggggctt gcggcgggtt tggccggcgg cagtttcagc ggccccttga
     5341 tgccgcacga tgtcagtgca agcgccgcga tgcatgcgat ggcgacggcg taacggcgta
     5401 acattcccgg ctccaggtgc aagaaagtca tcgctatgcg gccttcattg gcggaaagtc
     5461 tagcacgcac ccgcgggtcg atccgatgag cagcgagagc atgttcagcg ctagcgccga
     5521 cgcggttctc gccgccgtcg gcgcggcgct cgatgccgcc ggcgacgcca gcgacgccga
     5581 tctcgactgg tcgctgaacg acggggtgct gacgatcgat tgcgggcgcg acggcaagct
     5641 gatcgtcaat cggcatgtgc cgaatcgcga gatatgggtg gcggcgaaat ccggcggctt
     5701 tcactttcgt gccgccggcg gggcctggcg cgattcgcgc ggcggcgagg agcttggcgc
     5761 cgcactcacg cggctgctgc gggaccaggc cggcacgacc atcgacctga ctgcgctgcc
     5821 gccaccgccg gagg
//
LOCUS       DATANS010000449         4528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_275860, whole genome shotgun sequence.
ACCESSION   DATANS010000449 DATANS010000000
VERSION     DATANS010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275860"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /gene="dapB"
                     /locus_tag="VHT22_06620"
     CDS             <1..811
                     /gene="dapB"
                     /locus_tag="VHT22_06620"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX3137263.1"
                     /translation="VRVAIAGCGGRMGQTLIAAILGQQDMKLAAALEVAESPLLGHDA
                     GGPLGQVTGVVTGSDLAAALREADVLIDFTRPTATVAHVHACAGAGVGAVVGTTGLSE
                     ADKKALAEAGRTIPLVYAPNMSVGVNVLLKLVALAAQRLGGDYDVEIVEMHHRHKVDA
                     PSGTALQLGEAAAAAMGRDLKNDGVFARDGVIGERRPGAIGFAALRGGDVIGDHTVIF
                     AGPGERIELAHKASSRTTFAHGALRAARFVAAQRAANAPGLYDMRDVLGLD"
     gene            819..1598
                     /locus_tag="VHT22_06625"
     CDS             819..1598
                     /locus_tag="VHT22_06625"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX3137264.1"
                     /translation="MPAAHPFDLTGRLALVTGGGSGLGLAIARGLAAAGARIVLNGRN
                     RAKLEAAAASLERDGRANGGTEIAPFDVADADAVAAGIAAIERDFGALDIVVNNAALN
                     KRAPLDEFTPDDWHRLLAANLDGPFYVARSVMTGMKARRRGKIINICSLASEIGRPNI
                     VPYATSKGGLQMLTRALAVELAPFNVQVNGIAPGFFKTEMNTALIADAKFSAWVASRT
                     PTGRWGEPPEIAGAAVFLASGAADYVTGHVLFVDGGFSVAY"
     gene            1713..2888
                     /gene="carA"
                     /locus_tag="VHT22_06630"
     CDS             1713..2888
                     /gene="carA"
                     /locus_tag="VHT22_06630"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX3137265.1"
                     /translation="MTSASVPAILALADGSLFRGRAFGATDSTEGSIGEVVFNTAMTG
                     YQEILTDPSYAGQIVTLTYPHVGNTGVNPEDVESRRVFAAGLVIRDLPRLVSNWRSSQ
                     DLSSYLRDAGVLGICDIDTRKLTRMLRENGAQNGCVVVAGSSGRGDAAAEAIARARAA
                     PSMSGLDLAKVVSCDAPYDWNGGSWALGSGYHARQGGRFHVVAYDYGIKHNILRLLAD
                     RGCRLTVVPAQTPARDALALQPDGIFLSNGPGDPEPCTYAIDAIREFLAVGMPTFGIC
                     LGHQLLGLACGARTVKMKFGHHGANHPVLDKDTGQVLITSQNHGFAVEASTLPRNVRI
                     THVSLFDGSLQGIEFTDKPAFGFQGHPEASPGPHDLAFLFDRFTRMMERHAVKAVSA"
     gene            2991..3611
                     /locus_tag="VHT22_06635"
     CDS             2991..3611
                     /locus_tag="VHT22_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX3137266.1"
                     /translation="MSALTQDEAARVVNAALSESADALQALRADAPALAAIARAAALI
                     VDAFGRGGRVFSCGNGGSMCDAMHLAEELSGRFRGDRKGLPAQAISDAGYLSCVANDF
                     GYDAVFARYLESHARAGDILVAISTSGTSKTVLAAASAAAAIGMQTIVLTGRSGSPLA
                     AAADCTICTPAGRYADRVQELHIKVIHILIELIERDLFPENYGAAR"
     gene            3625..>4528
                     /gene="carB"
                     /locus_tag="VHT22_06640"
     CDS             3625..>4528
                     /gene="carB"
                     /locus_tag="VHT22_06640"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986498.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEX3137267.1"
                     /translation="MPKRTDIESILIIGAGPIIIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPDMADATYIEPITWPMVAKIIEKERPDALLPTMGGQTALNCALDL
                     AREGILTRFDVELIGATKKAIDKAEDREKFKAAMTRIGLGSARSGIAHDLEQALEVQA
                     DIGFPAVIRPSFTLGGTGGGIAYNKDEFVEMCKRGLASSPTHELLIEESLLGWKEFEM
                     EVVRDRKDNCIIVCSIENLDPMGVHTGDSITVAPAMTLTDKEYQRLRDAARAVMSEIG
                     VETGGANVQFAVRPGDGAVRVIEMN"
BASE COUNT          779 a         1503 c         1535 g          711 t
ORIGIN      
        1 ggtccgcgtc gcgatcgcgg gctgcggtgg ccgcatgggc cagacgctga tcgcggcgat
       61 cctcggtcaa caggacatga agcttgccgc cgcgctggag gtggctgaga gcccgttgct
      121 cggccacgat gccggcgggc cgctcggaca agtcaccggc gtggtgaccg gttcagatct
      181 cgccgctgcg ctccgcgaag ccgacgtcct gatcgacttc acccgaccga cggcgacggt
      241 tgctcacgtc cacgcctgtg ccggcgccgg cgtcggtgca gtggtgggca cgacgggcct
      301 gagcgaagcc gacaagaaag cgctggccga ggccggccga acgatcccgc tggtctatgc
      361 gccgaatatg agcgtcggag tcaacgtact gctcaagctg gtcgcgcttg ccgcgcagcg
      421 tctcggcggc gactatgacg tcgagatcgt cgaaatgcac caccggcata aggtggacgc
      481 gccctcaggc actgcgctgc agctcggtga agcggcagcc gcggcgatgg gccgcgacct
      541 gaagaacgac ggtgttttcg cgcgcgatgg tgtgatcggc gaacgcaggc cgggcgcgat
      601 cgggttcgcc gccttgcgcg gcggcgatgt catcggggat cacacggtga tattcgccgg
      661 gcccggcgag cgcatcgagc tcgcgcacaa ggcgtcgtcg cgcacgacgt tcgcgcatgg
      721 cgcgctccgc gcggcacggt ttgtcgcagc gcagcgcgcg gcgaacgcgc cgggtctgta
      781 cgacatgcgg gacgtcctcg gtctcgactg acggcgccat gcccgcggcg catcccttcg
      841 acctgaccgg gcggctggcg ctcgtcaccg ggggcggcag cgggctcggc ctcgcgatcg
      901 cacgcgggct cgccgccgcc ggcgcgcgca tcgtcctcaa cgggcgcaat cgcgccaagc
      961 tggaggcggc cgctgcttcg ctcgagcgcg atggccgtgc gaacggtggc accgaaatcg
     1021 cgccattcga tgtcgccgac gccgatgccg tcgccgccgg catcgccgcg atcgagcgcg
     1081 acttcggtgc gctggacatc gtcgtcaaca atgccgcgct gaacaagcga gcgccgctgg
     1141 acgaattcac gcccgacgac tggcacagac tccttgccgc gaatctcgat ggtccgtttt
     1201 acgttgcgcg cagcgtgatg accggaatga aagcgcgccg gcgcggcaag atcatcaata
     1261 tctgttcgct ggcgagcgaa atcggccgcc ccaatatcgt tccgtacgcg accagcaagg
     1321 gcggtctcca gatgttgacc cgcgcgctcg cggtcgagct cgcgccgttc aatgtgcagg
     1381 tcaatggcat cgctccgggc tttttcaaga ccgagatgaa tacagcgctg atcgccgacg
     1441 caaagttttc cgcctgggtc gcgtcgcgca cgccgaccgg acgctggggc gagccgcccg
     1501 aaatcgctgg agctgcggta ttcctcgcat cgggcgccgc agattacgtg accgggcacg
     1561 tgctgttcgt cgacggcgga ttcagcgtcg cgtattagcc accgcattga acgacgcgcg
     1621 ctgctagggt ataatttcca gccttcggag cggggaaggc tgcaggcctt tcccgcttcg
     1681 cacgtctgga cccgttttcg agaaactgtg ccgtgacttc cgcctccgtc cccgcgatcc
     1741 tggcgcttgc cgacggcagc ctgtttcgcg gccgcgcgtt cggcgcgacc gactcgaccg
     1801 agggcagcat cggcgaagtc gtgttcaaca ccgcgatgac cgggtaccag gagatcctga
     1861 ccgacccgtc gtatgccggc cagatcgtta cgctgactta tccgcatgta ggcaatacgg
     1921 gcgtcaaccc cgaagacgtc gaatcccggc gcgtttttgc cgccggactg gtgatacgcg
     1981 acctgccgcg tctggtcagc aactggcgca gcagtcagga cctgtcgtcc tatttgcgcg
     2041 acgcgggcgt cctcggcatc tgcgacatcg acacgcgaaa gctcacccgc atgctgcgcg
     2101 aaaacggcgc acagaacggc tgcgtggtcg tggcaggaag cagcggtcgc ggagacgccg
     2161 cggccgaggc gatcgcgcgc gcccgcgcgg cgccctcgat gtcgggactc gatctggcca
     2221 aagtcgtcag ctgcgacgcg ccgtacgact ggaacggcgg cagctgggcg ctcggcagcg
     2281 gctatcacgc gcgtcagggc ggtcggttcc acgtcgtcgc ctacgactac ggcatcaaac
     2341 acaacatcct gcgcctgctg gccgatcgcg ggtgccggct caccgtcgtc cctgcgcaga
     2401 ctcccgcgcg cgacgccttg gcgctgcaac ccgacggcat cttcctgtcg aacggccccg
     2461 gcgatcccga gccgtgcacc tacgcgatcg atgcgatacg cgaattcctc gccgtgggta
     2521 tgccgacgtt cggcatctgc ctgggtcatc agctgctcgg gctcgcctgc ggcgcgagaa
     2581 cggtcaagat gaagttcggc caccacggcg ccaaccatcc ggtgctcgac aaggacaccg
     2641 ggcaggtgct gatcaccagc cagaatcacg gcttcgccgt cgaagcgtcg actttgccgc
     2701 gcaacgtgcg aatcacgcat gtgtcgctgt tcgacggcag ccttcagggc atcgaattca
     2761 ccgacaagcc ggcgttcggc ttccagggcc atccggaagc aagcccggga ccgcacgatc
     2821 tggcgtttct gttcgatcgc ttcacgcgga tgatggagcg gcacgcggtg aaggcagtca
     2881 gcgcatgagc gaggcgagag cttgaagacc cgaagggccg tcgccgagcg agtgcgcgta
     2941 ggccgatccg cagatgcggt ccgttcgcga agcggcatgg aggccggcgc atgagcgccc
     3001 tcacgcaaga cgaggccgcg cgcgtggtga atgcggcgct gtcggaaagc gccgacgcgc
     3061 tgcaggcctt gcgcgccgac gcgccggcgc tggcggcgat agcgcgcgcc gcggcattga
     3121 tcgtcgacgc attcggccgc ggcggccgcg tgttcagttg cggcaacggc ggctcgatgt
     3181 gcgatgcgat gcatctggcc gaagagctgt cgggccgttt ccgcggagac cgcaagggct
     3241 tgcccgcgca ggcgatcagc gacgccggct acctttcctg cgtggccaac gacttcggct
     3301 acgacgcggt gttcgcccgc tatctcgaaa gccacgcccg cgcgggcgac attctggtcg
     3361 cgatcagcac cagcggaacc agcaagactg tgctggcggc ggccagcgct gcggcagcga
     3421 tcggaatgca gacgatcgtt ctgaccgggc gcagcgggtc gccgcttgcg gctgccgcgg
     3481 actgcacgat atgcacgccg gcgggccgct acgccgaccg cgtgcaggag ctgcacatca
     3541 aggtgataca catcctgatc gagctgatcg agcgcgatct cttccccgag aattacggcg
     3601 ccgcgcgctg actccaaagc tacgatgccc aagcgcactg acatcgagtc gatcctgatc
     3661 atcggcgccg gtccgatcat catcggccag gcctgcgaat tcgactattc aggagcacag
     3721 gcgtgcaagg cgctgcgcga agaaggctat cgcgtgatcc tggtcaattc gaatccggcg
     3781 acgatcatga ccgacccgga catggccgat gcgacgtaca tcgagccgat cacctggccg
     3841 atggtcgcga agatcatcga gaaggagcga ccggacgcac tgctgccgac gatgggcggc
     3901 cagacagcgc tcaattgcgc gctcgacctc gcgcgcgaag gcatcctgac ccggttcgac
     3961 gtcgagctga tcggcgcgac caagaaggcg atcgacaagg ccgaggaccg cgaaaaattc
     4021 aaggcggcga tgacgcgcat cggcctcggc tcggcgcgat caggcatcgc gcacgatctc
     4081 gagcaggcgc tggaagtgca agccgacatc ggcttcccgg cggtgatccg gccgtcgttc
     4141 acgctgggcg gcaccggcgg cggcatcgcc tacaacaagg acgaattcgt cgaaatgtgc
     4201 aagcgcgggc tcgccagctc gccgacgcac gagctgctga tcgaggaatc gctgctcggt
     4261 tggaaagagt tcgagatgga ggtcgtccgc gaccgcaagg acaactgcat catcgtctgc
     4321 tcgatcgaga acctggatcc gatgggcgtc cacaccggcg actcgatcac cgtcgcgccc
     4381 gcgatgacgc tcaccgacaa ggagtaccag cgcctccggg acgccgcgcg cgcggtcatg
     4441 agcgagatcg gcgtcgagac cggcggcgcg aacgtgcagt tcgcggtgcg gcccggcgac
     4501 ggcgcagtgc gcgtcatcga gatgaatc
//
LOCUS       DATANS010000450         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_192692, whole genome shotgun sequence.
ACCESSION   DATANS010000450 DATANS010000000
VERSION     DATANS010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192692"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VHT22_06645"
     CDS             <1..690
                     /locus_tag="VHT22_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HEX3137268.1"
                     /translation="NRSWNCGVEGPTDDPAIRALRERQKRNFLTTLLLSQGVPMLLGG
                     DEMGRTQQGNNNAYCQDSELSWIDWKLGPDAQNLIDFTHRLVALRNGHPLFRRRTFFH
                     GRSIAAADAKDIAWLNPDGQEITGDDWHKAFARCLGVFLSGHGLAESDAHGDPLDDDD
                     LLLLLNAHHEAIPFVLPKPRLTHWIVLLDTAIAADAPSPEPLPFGAPYPLQGRSMAVL
                     CSTPGDDETPA"
     gene            674..>1585
                     /locus_tag="VHT22_06650"
     CDS             674..>1585
                     /locus_tag="VHT22_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX3137269.1"
                     /translation="MKRRHEMGFGAMVLDDGAVRFRLWAPAARQVGLYLDGASHSLPL
                     AAGSDGWFELTTREAAHGTRYRYRIDDELSVPDPASRHNPQDVDGPSAVVDPFAFDWD
                     DEDWLGRRWEDAVVYELHVGTFTGAGTFAAIEAKLDHLCDLGVTALELMPIGDFSGTR
                     GWGYDGVLPFAPDASYGDPVALKQLVLAAHRRGLMVFLDVVYNHFGPVGNYLHRYAPQ
                     FFSSRHLTPWGHAINFDGEDSRVVRDFFIDNALYWLDEFHFDGLRLDAVHAIRDVDSA
                     PHFLDELAATVRRRCAGGRHVHLILEND"
BASE COUNT          260 a          525 c          516 g          284 t
ORIGIN      
        1 aaccgcagct ggaactgcgg cgtcgaaggc ccgaccgacg atccggcgat ccgtgcgctg
       61 cgagagcggc aaaagcgcaa tttcctgacg accctgctgc tgtcgcaagg cgttccgatg
      121 cttctgggcg gcgacgagat gggccgcaca cagcaaggca acaacaacgc gtattgccag
      181 gactcggagc tgtcgtggat cgactggaag ctcgggccgg acgcgcagaa cctgatcgat
      241 ttcacccatc ggctggtcgc gctgcgcaac ggccatccgc tgtttcgtcg gcgaacgttc
      301 tttcatggcc gctcgatcgc cgctgccgac gccaaggaca ttgcctggct caatcccgac
      361 ggccaggaaa tcaccggcga cgattggcac aaggcgttcg cgcgctgcct gggcgtgttc
      421 ctgtcggggc acggcctggc cgaaagcgat gcgcacggcg acccgctcga cgacgacgac
      481 ctgctgctgc tgttgaacgc gcatcacgaa gcgattccgt tcgtgctgcc gaagccgcgc
      541 ctgacgcatt ggattgtgct gctagacacc gctatcgccg ccgacgcgcc gtcacccgag
      601 ccgctgccgt tcggcgcgcc gtacccgttg cagggccgct cgatggcggt gttgtgcagc
      661 acgccgggcg acgatgaaac gccggcatga aatgggcttc ggggcgatgg tcctcgacga
      721 tggggcggtg cgcttccgcc tctgggcgcc ggcggcgcgc caagtcgggc tgtacctcga
      781 cggtgcatcg cattcgttgc cgctagccgc cggctcagac ggatggttcg agctcacgac
      841 gcgcgaagcg gcgcacggca cgcgctatcg ctatcggatc gacgacgaat tatcggtgcc
      901 cgatccagca tcgcggcaca acccgcagga cgtcgacgga ccgagcgccg tcgtcgatcc
      961 gtttgccttc gactgggacg atgaagattg gctcggccgg cgctgggaag acgcggtcgt
     1021 ctacgagctc catgtcggca cgttcaccgg cgccggcacg ttcgcggcaa tcgaggcgaa
     1081 gctcgaccac ctgtgcgacc tgggcgtgac cgcgctcgaa ttgatgccga tcggcgattt
     1141 cagcggcacg cgcggctggg gttacgacgg cgtcctgccg ttcgcacccg atgcgagcta
     1201 cggcgacccc gttgcgttga agcaactggt actcgcggcc caccgccgcg ggctgatggt
     1261 gttcctcgac gtggtctaca atcatttcgg gccggtcggc aactacctgc atcgctacgc
     1321 accgcaattc ttcagctcgc gccacctcac tccgtgggga catgcgatca atttcgatgg
     1381 cgaggacagt cgcgtcgtgc gcgatttctt catcgacaac gcactgtatt ggctcgacga
     1441 gtttcacttc gacggcttgc gcctcgatgc tgtgcatgcc attcgcgatg tcgactcggc
     1501 gccgcatttc ctcgacgagc tggctgcgac ggtgcgccgg cgctgcgccg gcggccgtca
     1561 cgtccacctg atcctggaga acgac
//
LOCUS       DATANS010000451         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_192744, whole genome shotgun sequence.
ACCESSION   DATANS010000451 DATANS010000000
VERSION     DATANS010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192744"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VHT22_06655"
     CDS             complement(<1..735)
                     /locus_tag="VHT22_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX3137270.1"
                     /translation="MRKLTYLLCCAALVGACASPPQKANEPAKKSEFAAVPPEPVNPY
                     PSTYKADTATATLIRGATVLTGTGTRLENADVLIVNGKIEAVGPNLSAPAGARVVDAS
                     GRWVTPGLIDVHSHLGVYASPGVNASSDGNEATDPVTANVWSEHGVWPQDPGFETALE
                     GGITSLQILPGSANLIGGRAVTLKNVPATTYQAMKFPDAPQGLKMACGENPKRVYGGQ
                     SRAPSTRMGNVAGYRQAFADAQDYREQ"
     gene            complement(763..1293)
                     /locus_tag="VHT22_06660"
     CDS             complement(763..1293)
                     /locus_tag="VHT22_06660"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX3137271.1"
                     /translation="MSAPTLTFTLATLPSPLGGMLIVTDSEQRLRALNWEDREDRMRR
                     ELVRIYGRKAVQLERGAAPTPIRRALQRYFAGKLQAIDEIDVETGGTPFQSNVWRGLR
                     TIPAGTTLSYGRMAQRLKCPLAVRAVGFANGSNPISVVVPCHRLVGADGSLTGYGGGL
                     ERKRWLLEHEGVRFGE"
     gene            1396..>1581
                     /locus_tag="VHT22_06665"
     CDS             1396..>1581
                     /locus_tag="VHT22_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019830832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137272.1"
                     /translation="MTAPSEPVGTLDTALDHTQRLMTSEPRLAAEQATEILKVAPGHP
                     LATLLLGVANRRAGDAAT"
BASE COUNT          260 a          528 c          514 g          279 t
ORIGIN      
        1 ctgctcgcga taatcctgcg catccgcgaa ggcctggcga tagcctgcga cgttgcccat
       61 gcgagtgctc ggcgcgcgcg attggccgcc gtagacgcgc ttcggatttt cgccgcacgc
      121 catcttcagt ccctgcggcg cgtccggaaa cttcatcgcc tgataagtcg tcgcgggtac
      181 gttcttcaac gtcaccgcgc ggccgccgat cagattggcg gagccgggca gaatctgcag
      241 cgaggtgatg ccgccttcga gcgcggtctc aaagcccgga tcctgcggcc acacgccgtg
      301 ctcggaccag acgttcgccg tgacgggatc ggtcgcctcg ttgccatcgc tgctggcgtt
      361 cacaccggga ctggcataga cgccgagatg cgaatgcacg tcgatgagac cgggagtgac
      421 ccagcgaccg ctcgcatcga cgacgcgtgc acctgccggc gcgctgagat tcgggccgac
      481 cgcttcgatc ttgccgttca cgatgagcac gtcggcgttc tcgagccgcg tacctgtgcc
      541 ggtcagcacg gttgcgccgc gaatcaacgt cgccgttgcc gtgtcagcct tgtacgtcga
      601 cggataagga ttgactggct cgggcgggac cgccgcaaac tcggacttct tcgctggttc
      661 gttggctttt tgcggcgggc tcgcgcatgc gccgaccagg gccgcacagc agagcaggta
      721 ggtgagtttg cgcatggccg cggaatgtat cagacctcac cgctactcgc cgaagcggac
      781 accttcgtgt tccagcaacc aacgcttgcg ttcgaggccg ccgccgtagc cggtcagcga
      841 accatctgcg ccgacaaggc gatgacacgg gaccacgacg ctgatcgggt tcgagccgtt
      901 cgcgaagccg accgcgcgca cggccagcgg acacttgagt cgctgggcca tgcgcccgta
      961 gctcagagtc gtgcccgccg gaatcgtgcg cagcccgcgc catacgttgc tttgaaatgg
     1021 cgtgccgccg gtctcgacgt cgatctcatc gatggcctgc agcttgcccg caaagtagcg
     1081 ctgcaaggcg cggcggattg gagtcggcgc cgcacctcgt tcgagttgca cggctttgcg
     1141 gccatagatc cgcacgagct cgcgccgcat gcggtcttcg cgatcctccc aattcagcgc
     1201 gcgcaggcgc tgctcgctgt cggtgacgat gagcatcccg ccgagcgggc tcggcagcgt
     1261 cgcgagggta aaagtcaacg tgggcgcgga catgggagga actctgcgcg cgccaggaca
     1321 ctggcggctc gccattttcg gacctcagtt cggacagggc gctatcatgc ccgcgcccga
     1381 cgtcgaagtt tgcgcatgac tgcaccctca gaacccgttg gaacgctcga taccgcgctg
     1441 gatcacacgc agcgcctgat gacctccgag ccgcgactcg cggccgaaca ggctacggaa
     1501 atcctgaagg tcgcgccggg ccatccgctg gcgacgcttt tgctcggcgt ggccaatcga
     1561 cgcgcgggtg atgcagccac t
//
LOCUS       DATANS010000452         1613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_653669, whole genome shotgun sequence.
ACCESSION   DATANS010000452 DATANS010000000
VERSION     DATANS010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653669"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..573
                     /locus_tag="VHT22_06670"
     CDS             55..573
                     /locus_tag="VHT22_06670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3137273.1"
                     /translation="MNIRRLTRIEARDLDGLCELLIDCVEGGASVSFMLPMTRGKALA
                     YWRDVAASATRCERIVLAAYDGTRNIVGTVQVVFAQPENQLHRADIAKMLVHRRGRRR
                     GVGAALLIAAERHALRSGKTVLVLDTASDDAERLYVRQGWRLCGRIPDYAFMPDGAPC
                     ATAVYYKSLRGP"
     gene            818..>1613
                     /locus_tag="VHT22_06675"
     CDS             818..>1613
                     /locus_tag="VHT22_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137274.1"
                     /translation="MKRGSIVALLLAHLTVASARPELPAAGLDALYPAYLRIPGSAGR
                     MLIFGGEGADAKANDAIYELAFARATASVTWTARGVLAVARREPEIAVFPSTNVVAIV
                     GGLSTQGSPLNAIETFDYATGKRTSIAARLPAGLVHHQVVPCGTGARVLVIGGDDGHA
                     AVASLTVVALDAATVEPLRDTAGRKVTLKAARSFVSAASIDDANSKLLVVGGETRGGA
                     ASNIGEIIEVTAECVAQRIRATSPLPERRTRGALTRTGNDSAIYYAG"
BASE COUNT          280 a          519 c          546 g          268 t
ORIGIN      
        1 cacgttccgc aaccgcacgc gcaaggtcgc gcgttacgtc gtggtgctgt ccgcatgaac
       61 atccggcgtc tcacgcggat cgaagcccgc gacctggatg gcctgtgcga gctgttgatc
      121 gattgcgtcg aaggcggcgc ctcggtgagc ttcatgctgc cgatgacgcg cggcaaggcg
      181 ctggcctact ggcgcgacgt ggccgcaagt gcgacgcgct gcgaacgcat cgtgctggca
      241 gcttacgatg ggacgcggaa catcgtcggc acggtgcaag tcgtgttcgc gcagccggag
      301 aaccagctgc atcgagctga cattgcgaag atgctggtgc atcggcgcgg gcgacggcgc
      361 ggagtcggtg cagcgttgct gatagccgcg gaacgtcatg cgctgagatc cggcaaaacg
      421 gtgctcgtgc tggacacggc ctcggacgat gccgagcggc tgtacgtgag gcagggctgg
      481 cgactctgcg gccgcatccc cgactacgcc ttcatgccgg acggcgcgcc ctgcgccacc
      541 gcggtctact acaaatcact gcgcggcccg tgaggcgcga cgacggccca attgcgtcgc
      601 gccacccagg ccgcgcgtga catccattgc ggggtcgcga aagtacggca gagcaaaaat
      661 ttggccatac gcgaacccca agcacgggag gtatggtgcg ctcagccgcg cgctgccgaa
      721 tcggcagcta ctacccaatt tacgcgtccg gcctacccat atttcgggag tcgggactac
      781 cgtggcgcga gcacgttcgg gccgccatga tgggcccatg aaacgtggat cgatcgttgc
      841 gctgctgcta gcgcatttga cagtcgcgag tgcccgtccc gagctgccgg cggcgggcct
      901 cgatgcgctc tacccggcgt acctgcgaat tccagggtct gcgggccgca tgctgatctt
      961 cggcggcgaa ggcgcggatg cgaaggcgaa cgacgccatt tacgagctcg cgttcgcacg
     1021 tgcgaccgcg tctgtgacat ggacggcgcg aggtgtgctc gctgtcgcga ggcgcgagcc
     1081 ggaaattgcg gtctttccca gcaccaatgt tgtcgccatc gtcggcggct tgagcacgca
     1141 gggctcgccg ctgaatgcga tcgaaacgtt cgactacgcg acgggcaaga ggacgtcgat
     1201 cgcggccagg ctgccggccg gtctcgtgca ccaccaggtc gtgccctgcg gtacgggtgc
     1261 acgcgtgctc gtcatcggcg gcgatgacgg acacgccgcg gtcgcaagcc tgactgtcgt
     1321 cgcactcgac gccgcgactg tcgagccact tcgcgacacc gctggccgca aggtcacgct
     1381 caaagccgca cgcagttttg tcagtgctgc ctcgatcgac gacgcgaaca gcaagctgct
     1441 cgttgtcggt ggagagacgc gcggtggtgc cgcctcgaac atcggcgaga tcatcgaagt
     1501 cacagcggag tgcgtagcgc aacggatcag agcgacgtct cctcttcccg agcgacgaac
     1561 acgtggcgcc ctgacccgaa ccggcaacga ttcggcgatc tactacgccg gct
//
LOCUS       DATANS010000453         7212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_569013, whole genome shotgun sequence.
ACCESSION   DATANS010000453 DATANS010000000
VERSION     DATANS010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569013"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VHT22_06680"
     CDS             <1..345
                     /locus_tag="VHT22_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX3137275.1"
                     /translation="RCRAALPSQHPLWSYVDRGFYCGQLRRIWRYFPVEQTLILKSEE
                     LRTAPEHLLASIAAFLGIESFGPVERREVYARDYDEPMPDEARNYLRDIFEYEIRSLE
                     RMLGWDCSDWLA"
     gene            complement(355..1149)
                     /locus_tag="VHT22_06685"
     CDS             complement(355..1149)
                     /locus_tag="VHT22_06685"
                     /inference="COORDINATES: protein motif:HMM:NF039976.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6065 family protein"
                     /protein_id="HEX3137276.1"
                     /translation="MSPSPDGCLPTSMKLSCYALRPDPLTIRPAPLTRPWMDRIADNH
                     AYRCLPLNIANSHGWEVLSPCAFSATWSGGIHARDLKLTANDGYPRLADFAVTHFAYG
                     IVTFHLSYLFRTEPGWDLFATGPLNGGKDGIAPLTGVIETDWLPYPFTMNWQLTRPGT
                     VRFDKDEPICMLFPVPHGALQTFEPEIMNLDDDPELKAQTFALKERRDEFMKKFNDND
                     AQTLKQAWQRFYFVGKMPDGSQPEQHLHKLRLAAPVDRRKPPATKA"
     gene            1288..2985
                     /gene="gspE"
                     /locus_tag="VHT22_06690"
     CDS             1288..2985
                     /gene="gspE"
                     /locus_tag="VHT22_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEX3137277.1"
                     /translation="MNAPERKRLGEILIERGKLDAAGLDRALRIQSDSGEKLGVLLVT
                     IGVVAQRDVCEALANQLGLPLVEAASYPELPILEERVSARFLRESRALPLAEDETELA
                     LAMADPTDEYTVNAFEMVTGRKVRPQVAIPMELEAALERLYGAGKTALGQILGDVEQR
                     DDLAFDADVQQLKDLASEAPVIRMVSLIITNALDTRASDIHIEPFENRLIVRYRIDGV
                     LHEVESPPRRLSAAVISRIKIMANLDIAERRLPQDGRIRLRVQGKEIDLRVSTVPTMH
                     GESVVMRILDKGGVALDFKRLGFEEDTLKKFLNVLMEPHGILLVTGPTGSGKTTTLYT
                     ALDRLNQPDVKILTVEDPVEYQMAGINQIQVKPQIDLTFANALRSIVRQDPDVIMIGE
                     IRDLETAQIAVQSALTGHLVLSTVHTNDAASTVNRLLDMGVEDYLLTSTVIGILAQRL
                     VRTLCPACKTSYTALPEVVAELGLHKFAPPGDITLYHAKGCKECAQTGFIGRISIMEM
                     LPMSDPLRTHIMRHANSAELRTEAIREGMVTMYEDGMRKALNGSTTFEEVLRVTREN"
     gene            3050..4282
                     /locus_tag="VHT22_06695"
     CDS             3050..4282
                     /locus_tag="VHT22_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018175272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX3137278.1"
                     /translation="MALYKYRAVNAAGDVAAGELEAANESEIVGRLRDQGLMPMRVEA
                     AIGGKMAGTAPKSKRRRWFAARRVTRDNLLSITRELATLLRAGLPLDRALEVLIGLAP
                     TPSVASLLQTIRDDVRGGKALSQALDAHRTVFSRFYVNIVRAGEAGGALGTVLTRLAD
                     TMERTKDLRDSVQSALIYPTILIGVAVTSVMVLLVFVVPQFQQTFSQAGKALPLPTQI
                     VILFGTALRKYWWLAILIVVGLIWLIRRRLQKPEVRFRWDGRLLRLPLLGDLLGKVEV
                     ARFSRTLATLLANGVTLLSGLAIVRETMTNTVLARALDGVITRLREGKGFGRPLVETG
                     LYPKLATQMILVGEESGRLEEMLNRVADVYDREVQISIKRFLAILEPAMILGLAVLIG
                     GIVFSILLGVMGMSELVS"
     gene            4418..4888
                     /gene="gspG"
                     /locus_tag="VHT22_06700"
     CDS             4418..4888
                     /gene="gspG"
                     /locus_tag="VHT22_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019570495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEX3137279.1"
                     /translation="MFESSVAHRRAPGRARRASASARGMTLIEILVVLVLIGIVMGIV
                     GGNYIGQGEKAKARAARIEIEQIGQTLDLFKLEVGRYPTSQEGLQALVTAPAGMTNWN
                     GPYWKKSSVPKDPWGNEYQYASPGSHGPYDIISYGADGKEGGEGGNKDVTSWDQ"
     gene            4885..5388
                     /locus_tag="VHT22_06705"
     CDS             4885..5388
                     /locus_tag="VHT22_06705"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEX3137280.1"
                     /translation="MTAVQGFAYRFADTPSAGVDAMRTASGFTLIEMLVVLTLIGLLV
                     AVVIPMFGPGVSTTELKSDARAVAAGLRQARGQAIAQRGEAVLMLDLGAHSFSVPPDP
                     RVHKLAQELELKLFTAQSDLINDNVGAIRFFPDGGSNGGRITLASGERKFNVDVDWLT
                     GRVAILE"
     gene            5397..5822
                     /locus_tag="VHT22_06710"
     CDS             5397..5822
                     /locus_tag="VHT22_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX3137281.1"
                     /translation="MFSSAPRGFSLIEVLAAFVILALVGTALFRIFAGALGNASLSDE
                     YSRATLFAESRLASPGVETPLREGAQQGTSDDGKYAWTSRIEPYRPPGTTPDLDAATD
                     LMPMRLWRIAVTVSWPGVAGSDRSIALATVRLAAKELTP"
     gene            5819..6517
                     /locus_tag="VHT22_06715"
     CDS             5819..6517
                     /locus_tag="VHT22_06715"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX3137282.1"
                     /translation="MTELQVTTAEPKRRIAGFTLIELMVALLLLALMSSVLYGSLSLS
                     ATSWDRGEAKAEQAGDMRQTAEFLRQALGAEHPLRLHKAVDQPLVFAGASDSLAFAGA
                     TPGRVGGGIYYFRIALAGGGESSKLLLSRTIPDYSALKAPSFDGTDASVLADGIAQVK
                     FSYFGRDADANDAVEPTWRDRWDDPQVLPLLIRMDVKTAQGASWPSLVVEPRIAPEAG
                     CRGWDPNRRRCVGP"
     gene            6522..>7212
                     /locus_tag="VHT22_06720"
     CDS             6522..>7212
                     /locus_tag="VHT22_06720"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137283.1"
                     /translation="MNARIVISESAAPPAQRGIALVLVLWLTALLTVIAGGFAYAMRN
                     EAQAARNTMSQAQARALADGAIARTAFELMRPRTIPGVWAADGLVHVWDEDGMRLAAS
                     AVDESGKIDLNTVPDALLNSLLQTAGQVDADTAARLVDVIDDWKDADDFRRPNGAEAA
                     EYQAAGLPYKPANAAFETVADLQRVLGMTPALFARLADSLTVYSGAPGISPQFASRTV
                     LMALPGATDAIV"
BASE COUNT         1244 a         2278 c         2426 g         1264 t
ORIGIN      
        1 cgttgccggg cggctctccc gtcgcaacac ccgctctggt cttatgtcga tcgcggcttc
       61 tactgtgggc agctgcgacg gatctggcgc tatttcccgg tcgagcagac tctgatcctc
      121 aagagtgaag agctgcggac cgcgccggaa cacctgctcg cgagcatcgc cgcatttctg
      181 ggaatcgaat ctttcgggcc ggtcgagcgc cgcgaagtct acgcgcggga ctatgacgag
      241 ccaatgcccg acgaagcgcg caattacctc cgagatattt tcgaatacga aatccggtcg
      301 ctggagcgaa tgctcggctg ggattgctcg gactggctgg cgtaggcgcg cccgctaagc
      361 cttggtcgcc gggggtttgc gccgatcgac cggtgcggcg agacgaagct tgtgcagatg
      421 ctgctcgggc tgcgatccgt cgggcatctt gccgacgaaa tagaatcgct gccacgcctg
      481 cttcaatgtc tgcgcgtcat tgtcgttgaa cttcttcatg aactcgtcac ggcgctcctt
      541 caaggcaaac gtctgcgcct tgagctccgg atcgtcgtcg agattcatga tttccggctc
      601 gaacgtctgc agcgcaccgt gcggcaccgg aaagagcatg cagatgggct cgtccttgtc
      661 gaagcgcacg gtgcccggac gcgtcagctg ccagttcatc gtgaacggat aaggcagcca
      721 atcggtttcg atcacccccg tcagcggcgc gatgccgtcc ttgccgccgt tcagcggtcc
      781 ggttgcgaac agatcccagc ccggctcggt gcgaaacagg tacgacaggt gaaaggtgac
      841 gatgccgtag gcgaaatgcg tgaccgcaaa atcggcgagc cgcggataac cgtcattggc
      901 cgtcagtttc aggtcgcgtg cgtgaatgcc gcccgaccac gtcgcgctga acgcgcacgg
      961 cgagagcact tcccagccat ggctgttggc gatgttgagc ggcagacagc gataggcatg
     1021 gttgtcagcg atccggtcca tccacggccg ggtcagcggc gcgggccgga tggtcaacgg
     1081 atcgggtctg agcgcataac agctgagctt catcgaagtc ggcaggcaac cgtcggggga
     1141 aggcgacatt ctactcggcg cacgtcgtcc gacggcgaca gtcggtgctg acaaacggct
     1201 gcaaaccatt gattcgaccg gcttcgccga cttctgccct cgctcgacat gctatgatcg
     1261 cgcgttgatt tttatgcggc ggcaggcgtg aacgcacccg aaagaaagcg actcggcgag
     1321 atcctgatcg agcgcggcaa gctggatgcc gccggcttgg accgcgcctt gcgcatccag
     1381 agcgactccg gcgagaagct cggcgtgctg ctggtcacga tcggcgtggt cgcccagcgc
     1441 gacgtgtgcg aggcgctggc caatcagctt ggattaccgc tggtcgaggc ggccagctac
     1501 cccgagcttc ctatcctcga ggagcgcgtg tcggcgcgat tcctgcgcga atcgcgcgcg
     1561 cttccgctcg ccgaagacga aaccgagctg gcgctggcga tggccgaccc gaccgacgag
     1621 tacacggtca acgcgttcga gatggtcacc gggcgcaagg tgcgaccgca ggtcgcgatt
     1681 ccgatggagc tcgaggcggc gctagagcgg ctgtatggtg cgggcaaaac ggcgctcggc
     1741 cagatcctgg gcgatgtcga gcagcgcgac gatcttgcct tcgatgccga cgtccagcaa
     1801 ctgaaggacc tcgcctccga ggcgccggtg atacgcatgg tgtcgctgat catcaccaat
     1861 gcgctcgaca cgcgagcgtc ggacatccac atcgagccgt tcgagaaccg gctgatcgtg
     1921 cgctaccgga tcgacggcgt gctgcatgag gtcgagtcgc cgccgcgtcg cctttccgca
     1981 gcggtgatct cgcgcatcaa gatcatggcg aatctcgaca tcgccgagcg gcgtctgccg
     2041 caggatgggc gcatccggct gcgcgtgcag ggcaaggaga tcgacctgcg cgtatcgacg
     2101 gtgccgacga tgcacggcga gtccgtagtg atgcgtatcc tcgacaaggg cggcgtcgcg
     2161 ctcgacttca agcggctcgg cttcgaggaa gacacgctga agaaattcct caacgtgctg
     2221 atggaaccgc acggcatcct gctcgtcacc gggccgaccg gcagcggaaa gacgacgacg
     2281 ctgtatacgg cgctcgatcg cctgaaccag cccgacgtca agatactcac cgtcgaggac
     2341 ccggtcgaat accagatggc cgggatcaac cagatccagg tcaaaccgca gatcgatctg
     2401 acgttcgcca atgcgctgcg gtcgatcgtg cggcaggatc ctgacgtcat catgataggc
     2461 gagatccgcg acctcgaaac cgcgcaaatc gcagtgcagt cggctctgac cggtcacctg
     2521 gtgctatcga ccgttcacac caacgatgcc gcgagcaccg tcaaccgcct gctggacatg
     2581 ggcgttgagg actatctgct gacatcgacg gtgatcggca tcctggcgca gcgactggtc
     2641 cggacgctgt gcccggcgtg caagacctcg tacacggcgc tgccggaggt cgtcgccgag
     2701 ctgggcctgc acaagttcgc gccgccgggc gacatcacgc tatatcacgc caaggggtgc
     2761 aaggagtgcg cgcaaacggg attcatcgga cggatcagca tcatggaaat gctgcccatg
     2821 agcgatccgc tgcgcacgca catcatgcgc cacgccaatt cggcggagct tcgcaccgag
     2881 gcgatcaggg aaggcatggt tacgatgtac gaggacggca tgcgcaaggc gctcaacggc
     2941 tcgacgacgt tcgaagaggt tcttcgcgtc acgcgcgaaa actagtgtcg ggttgcggca
     3001 acatggtctc gtgcttacgg tggcggctgc acacggccgc ggtccgctga tggcgctgta
     3061 caaataccgg gcggtcaatg ccgccggcga cgtcgccgcc ggcgagctgg aagcggccaa
     3121 cgagtcggag atcgtcggcc ggctgcgcga ccagggactg atgccgatgc gggtcgaggc
     3181 cgcgatcggc ggcaagatgg ccggtactgc gccgaagtcg aaacgccggc gctggttcgc
     3241 agcgcggcgc gttacgcgcg acaatctgct gtcgatcacc cgcgagcttg cgaccttgct
     3301 tcgcgcgggg ctgccgctcg atcgcgcact cgaagtgctg atcggcctgg cgccgacccc
     3361 ctcggtggcg agcctgctgc agacgatccg cgacgacgtc cgcggcggca aggcactgtc
     3421 gcaggcgctc gacgcgcatc gcacggtgtt ctcacgcttt tacgtcaaca tcgtccgtgc
     3481 cggagaggcg ggcggcgcac tcggcacggt gctgacgcgg ctggccgaca cgatggagcg
     3541 gaccaaggat ctgcgcgaca gcgtgcagtc ggcgctgatc tacccgacga tactgatcgg
     3601 agtcgcggtc acttcggtga tggtgctcct ggtgttcgtc gtgccgcagt tccagcagac
     3661 attctcgcaa gccggcaagg cgctgccttt gccgacgcag atcgtcatcc tgttcgggac
     3721 ggcgctgcgc aagtactggt ggctcgcgat cctgatcgtc gtcggcctga tctggctgat
     3781 ccgccgccgg ctgcagaagc ctgaggtgcg cttccgctgg gacgggcgcc tgctgcgact
     3841 gccgctgctc ggcgatttgc tcggcaaggt ggaggtcgcg cgattttcgc gcacgctggc
     3901 gacgctgctt gccaacggcg tgacgctgct gtcaggcctc gcgatcgtgc gcgaaacgat
     3961 gaccaacacc gtgctcgcgc gtgcgctcga tggcgtcatc acgcgactgc gcgaaggcaa
     4021 gggcttcgga cggccgctgg tcgagaccgg tctctatccg aagctcgcga cgcagatgat
     4081 cctggtcggc gaggagtcgg gacggctgga ggaaatgctc aaccgcgtcg ccgacgtgta
     4141 cgaccgtgaa gtgcagattt cgatcaagcg gttcctcgcg atactcgagc cggcgatgat
     4201 actgggtctt gccgtgctga tcggcggcat cgtgttctcg atcctgctcg gcgtcatggg
     4261 gatgagcgaa cttgtcagct agacctgcct ctaatcgaac cgcccgcctg cgggcggagg
     4321 agaagtaacc atgcagttga atcatcatcg atcccaatcc cgcgtgctcg ctgcacgatc
     4381 acgcgtgctc cctccaccac cggaggagaa agctttcatt ttcgagtcgt cggtcgctca
     4441 tcggcgcgct ccgggtcgcg cccgtcgggc ctccgcctcc gcacgcggta tgaccttgat
     4501 cgaaatcctt gtcgtgctgg tgctgatcgg catcgtgatg ggcatcgtcg gcggcaatta
     4561 catcggccag ggcgagaagg cgaaggcgcg ggcggcgcgg atcgaaatcg agcagatcgg
     4621 ccagacgctg gacctgttca agctcgaagt cggccgttat ccgacgtcgc aggaaggttt
     4681 gcaggcgttg gtcaccgcac cggctggaat gacgaactgg aacgggccgt actggaagaa
     4741 atcgtcggtg ccgaaggatc cgtgggggaa cgaataccag tacgcgtctc cgggctcgca
     4801 cgggccgtac gacatcatct cgtacggcgc cgacggcaag gaaggcggcg agggtggcaa
     4861 caaggacgtc accagctggg atcagtgacc gccgtgcaag gattcgctta tcgctttgcg
     4921 gacacgccat cggccggcgt cgatgcgatg cgcaccgcat ccggcttcac gctaatcgag
     4981 atgctggtcg tgctgacgct gatcggcctg ctggtcgcgg tcgtgatccc gatgttcggc
     5041 cccggtgtat cgaccaccga gctcaagagc gatgcgcgcg ccgtcgccgc cggtctgcgc
     5101 caggcgcgcg gacaggcgat cgcgcaacgc ggcgaggcgg tgctgatgct cgatctcggc
     5161 gcgcacagct tcagcgttcc gcccgacccg cgcgtgcaca agctcgccca agagctcgag
     5221 ctcaagctct ttaccgcgca gagcgacctg atcaacgaca atgtcggcgc gatccgtttt
     5281 ttccccgatg gcgggtcgaa cggcggccgc atcacgcttg cctcggggga acgcaagttc
     5341 aacgtcgacg tcgattggct gaccggccgc gtcgcgatac tcgaatgaag cggccggtgt
     5401 tttcgtcggc accgcgaggc ttttcgctga tcgaggtcct ggcggcattc gtcattctgg
     5461 cgctggttgg aacggctctg ttccgcatct ttgccggtgc gctgggcaat gcgtcgctgt
     5521 cggacgaata cagccgcgcc acgctcttcg ccgaaagccg gctcgcgtcg cccggcgtcg
     5581 agacgccgtt gcgcgaaggc gcgcagcaag gcacgtccga cgacggcaag tatgcgtgga
     5641 cctcgcgcat cgaaccgtac cggccgccgg gcaccacgcc cgatctcgac gcggcgaccg
     5701 atctgatgcc gatgcgcctg tggcgtatcg cagtgaccgt cagctggccc ggcgtcgcag
     5761 gcagcgatcg ctcgatcgcg ctggccaccg ttcgtctcgc ggccaaggag cttacgccat
     5821 gactgagctc caggtgacga ccgccgaacc gaagcgccgt attgccggct tcacgctgat
     5881 cgagctgatg gtcgcgctgc tgctgctcgc gctgatgtcg agcgtccttt acggctcgct
     5941 gtcgctgtcg gcgaccagct gggaccgcgg cgaggcgaag gccgagcagg cgggcgatat
     6001 gcggcagacc gccgagttcc tgcgccaggc actcggcgcc gagcatccgc tgcgcctgca
     6061 caaggcagtc gatcagccgc tggttttcgc cggcgccagc gactcgctgg cgttcgccgg
     6121 ggcaacaccg ggaagagtcg gcggcggcat ctactacttc cgtatcgcgc ttgcgggggg
     6181 cggcgaatcc tccaagctgc ttttgtcgcg cacgattccc gattacagcg cgctcaaggc
     6241 gccgtcgttc gatggcaccg acgcctcggt gctcgccgac ggtatcgcgc aggtcaaatt
     6301 ttcgtatttc ggccgcgatg ccgatgcgaa cgatgcggta gagccgacct ggcgcgatcg
     6361 ttgggacgat ccgcaggtcc tgccgctgct gattcgcatg gatgtcaaga cggcgcaggg
     6421 cgcgagctgg ccgtcactgg tggtcgagcc caggatcgcg ccggaggcgg gttgccgcgg
     6481 ctgggatccc aaccgcagac gatgcgtggg gccctaaggc gatgaacgcg cgtatcgtca
     6541 tttcggaatc ggccgcgccg ccggcgcagc gcggcatcgc gctggtgctg gtcctgtggc
     6601 tgacggcgct gctgacggtg attgccggcg gcttcgccta tgcgatgcgc aacgaagcgc
     6661 aggcggcgcg caacacgatg tcgcaggcgc aggcgcgtgc cctcgccgat ggcgcgatcg
     6721 cgcgcacagc ctttgaattg atgcggcctc ggacgatacc cggcgtctgg gccgccgatg
     6781 ggctggtgca cgtctgggac gaggatggga tgcggctcgc ggccagcgcg gtcgacgagt
     6841 ctggtaaaat cgacctcaac actgtaccgg acgcgttgct caacagtttg ctgcagacag
     6901 ccgggcaggt cgacgccgat accgcggcgc gcttggtcga cgtgatcgac gattggaagg
     6961 acgccgacga tttccggcgg ccgaacggag ccgaagcggc cgagtatcag gccgccggcc
     7021 tgccgtacaa gcccgcgaac gccgccttcg agacggttgc cgatctacag cgcgttctgg
     7081 gcatgacgcc ggcgctgttc gcgcgacttg cggacagcct gaccgtctac agcggggcgc
     7141 cggggatcag tccgcagttc gcgtcacgca ccgtcctgat ggcgctgccg ggagcgaccg
     7201 acgccatcgt cg
//
LOCUS       DATANS010000454         1617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_401883, whole genome shotgun sequence.
ACCESSION   DATANS010000454 DATANS010000000
VERSION     DATANS010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401883"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1617
                     /locus_tag="VHT22_06725"
     CDS             <1..>1617
                     /locus_tag="VHT22_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HEX3137284.1"
                     /translation="ERLDATNPCGEQPLPAYGCCCLGSINLTLFVNDAFRPKARFDFA
                     AFARVIHPAVRMLDNVLDVTVWPLPQQHAEARAKRRVGLGFTGLGDTLIMLGLKYDTD
                     EARRMGAAIAEFMRDESYAASAAIAQEKGAFPLFDAKQFLAAPRFASRLPAELQKKIA
                     AHGMRNSHLLSIAPTGTISLAFADNASNGIEPPYSWTYQRKKRMPDDTHRTYDVEDHA
                     WRLFRHSGGEMDKLPAHFVTALEISALDHMRMVAAIAPFVDSAISKTVNVPEDYPYPE
                     FEDLYFQAWKAGLKGIATYRPNSVLGSVLSVTSTPATPEPNDLDTSDTDRRIRLDATP
                     EPALLSLRWPGRPVLPAGNAAWTYMVESPFGRFAIFVGHVENGAPYPFEVWINGNEQP
                     RGLGAIAKTMSMDMRALDAKWLRMKLDLLARTGGDAFDCAMPPEGKPLRVPSIVAAFA
                     RIVRYRVDKLGALVEAGGKSPVLDALFARKEPKTGTDGTMSWTVDVYNPSSGDDFVLM
                     LKELVLPNGQRRPYSMWMAGMYPKALDGLCK"
BASE COUNT          285 a          527 c          525 g          280 t
ORIGIN      
        1 gagcggctcg atgcgactaa cccttgcggc gagcagccat tacccgccta cggctgctgc
       61 tgcctcggca gcatcaatct gacgctgttc gtaaacgacg cgttccggcc gaaggcgcgt
      121 ttcgatttcg ccgcgttcgc gcgggtcatc catccggccg tgcgcatgct cgacaatgtg
      181 ctcgacgtca ccgtgtggcc gctgccgcag cagcatgccg aagcgcgcgc caagcggcgg
      241 gtcggcctag gtttcacggg cctcggcgac actctgatca tgctcgggct caagtacgac
      301 accgacgaag cgcgccgcat gggggccgcg atcgccgagt tcatgcgcga tgaatcttac
      361 gcggcgtcgg ccgcgatcgc gcaggagaaa ggcgcgtttc cgctgttcga tgcaaagcag
      421 tttctcgcgg cgccgcggtt cgcctctcgg ctgccggcgg aactccagaa gaaaatcgcc
      481 gcgcacggca tgcgcaacag ccacctgctg tcgatcgcgc cgaccggcac catttcgctc
      541 gcgttcgccg acaacgcatc caacggcatc gagcctccgt actcgtggac ttaccagcgc
      601 aagaagcgca tgcctgacga cacgcaccgt acgtacgacg tcgaggatca cgcgtggcgg
      661 ctcttccgtc atagcggcgg cgagatggac aagctgccag cccattttgt gacggcgctg
      721 gagatttccg cgctcgatca catgcgcatg gtcgcggcca ttgcgccgtt cgtcgattcg
      781 gcgatcagca aaaccgtcaa cgtgccggaa gactatccgt acccggaatt cgaagacctt
      841 tatttccagg cctggaaggc aggcctgaaa ggcattgcga cttaccgccc gaactcagtg
      901 ctgggcagcg tgctgtccgt cacttcgacg ccggcgaccc cggagccgaa cgatctagac
      961 acgtcggata ccgaccgccg cattcgcctc gatgctacgc ccgagcccgc gttgttgagc
     1021 ctgcgctggc cgggccggcc ggtgctgccg gcgggcaatg cggcgtggac ttacatggtc
     1081 gaatcgccgt tcggccgctt cgcgattttc gtcggacacg tggaaaacgg cgcgccgtat
     1141 ccgttcgaag tctggatcaa cggcaacgag cagccgcgcg gactgggcgc gattgccaag
     1201 acgatgtcga tggacatgcg cgcgctcgac gcgaaatggc tgcgcatgaa gctcgacctg
     1261 ctcgcgcgga ccggcggcga cgcgttcgat tgcgcgatgc cgcccgaggg caagccgctg
     1321 cgcgtgccga gcatcgtcgc ggcatttgcc cgcatcgtgc gctatcgcgt cgacaagctg
     1381 ggcgcgctgg tcgaagccgg cggcaaatcg ccggtgctcg acgcgttgtt cgcgaggaag
     1441 gaacccaaga ccggcaccga cggcacgatg tcgtggacgg tcgacgtcta caatccgagc
     1501 tcgggagacg atttcgtgct gatgctgaag gagctcgtgc tgccgaacgg ccagcgccgt
     1561 ccgtactcga tgtggatggc cggcatgtat ccgaaggcgc tcgacgggct gtgcaag
//
LOCUS       DATANS010000455         2985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_526806, whole genome shotgun sequence.
ACCESSION   DATANS010000455 DATANS010000000
VERSION     DATANS010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526806"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..114)
                     /locus_tag="VHT22_06730"
     CDS             complement(<1..114)
                     /locus_tag="VHT22_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX3137285.1"
                     /translation="MRILLSNDDGYFAPGLERLAADLAPYADITVVAPERDR"
     gene            complement(342..974)
                     /locus_tag="VHT22_06735"
     CDS             complement(342..974)
                     /locus_tag="VHT22_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016983989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX3137286.1"
                     /translation="MYTLYGKKGSGSATIEAALTMAGAPYRMVETASWETNDAFRDLL
                     KINPIGQIPTLALPDGSALSESAAILIHLDAAHPGAGLLPRDESARAQAIRGLVFIAA
                     NCYSAITVLDFPERFYQDIDDAARDHLRAGTKARLHRHWEIFADMFPARPFLAGNDMG
                     ALDLYAAVVTKWSGSRAHLAQHRPPFHAALLKVEAHPKVASVFARNWPAK"
     gene            complement(1106..1576)
                     /locus_tag="VHT22_06740"
     CDS             complement(1106..1576)
                     /locus_tag="VHT22_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011398549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3137287.1"
                     /translation="MVQPIPAGYAGVTSYLIIRDAASAIDYYKKAFGATEVMRFPGPD
                     GKVGHAELKIAGGNIMLADESPEMGHRSVQSYGGSPVSLMFYVPDVDKSFAQAIAAGG
                     KVKNPVKDQFYGDRSGTLTDPFGLMWTIATHKEDVSVADMQKRMEKMMADGQNA"
     gene            complement(1638..2330)
                     /gene="lexA"
                     /locus_tag="VHT22_06745"
     CDS             complement(1638..2330)
                     /gene="lexA"
                     /locus_tag="VHT22_06745"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008988213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX3137288.1"
                     /translation="MPHRPLANLRRSPAAPATPLAPRPTARQQQILDWIRGFIESRGM
                     PPTRAEIAAGLGFSSASSAEDHLQALAKKGALELVPGSSRGLRLKDIAGVPVQGTLPL
                     VGRVAAGQPILAAENVEARYRVDAALFSPRADYLLRVRGMSMKDVGILDGDLLVVHRT
                     ADARSGQIVVARLSGAAGDEVTVKRLRRRGREIVLHPENSEFEPIVVNPAHTDLAIEG
                     IAVGLLRTAALK"
     gene            2418..2969
                     /locus_tag="VHT22_06750"
     CDS             2418..2969
                     /locus_tag="VHT22_06750"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX3137289.1"
                     /translation="MSNPVLSFFRSLGDARVNASEDAMDVGSAVRRLLITAGTTALFA
                     SSPFAIADAYTDRIVMRGALPYDRSYEQLSPEQKAVVRAQYDSVGPNDEPPYPKYGLS
                     DVADAVARMPRREPLEGEVILTVSVDERGNAKSVSIYKTPDAKLSNVVAATLTRSKFK
                     PGLCERQPCAMDYVFKYYFKISA"
BASE COUNT          504 a          942 c          975 g          564 t
ORIGIN      
        1 gcggtcgcgc tcgggcgcta cgacggtgat gtcggcgtac ggagcgagat ccgcggcgag
       61 gcgctccagt ccgggggcaa agtagccgtc gtcgttgctg agcaggatgc gcatcgcgtg
      121 ggttggcgga ttcgcgccgg ccgtttcagg caccggcacg ctgcgtccat tatatgcgaa
      181 ggctgcgcgg aatcacgcga gcgatcgatg ccgctcgctc cggaccgtcg gctcgccgcg
      241 cggagcgcat cggcgtcacc gcgcttttgt tcggacccgc gctttctctt gcgtccggac
      301 ttgcgtacgc atttgcgtcc gggtctgggt tcgcgtttgc cttatttggc cggccagttg
      361 cgcgcaaaca cggaagccac tttcggatgc gcttcgacct tgagcagcgc cgcgtgaaac
      421 ggtggccgat gctgggcgag gtgtgcgcgc gaacccgacc acttggtcac gaccgccgca
      481 tagagatcca gcgcgcccat gtcgttgccg gcaaggaacg gccgcgccgg aaacatgtcg
      541 gcgaagattt cccagtgccg gtgcaggcgc gccttggtgc cggcgcgaag gtgatccctg
      601 gcggcgtcgt cgatgtcctg atagaagcgc tcgggaaaat cgagtacggt gatcgccgaa
      661 tagcagttag cggcgatgaa taccagtccg cggatcgctt gtgcgcgcgc cgactcgtcg
      721 cgcggcagca ggccggcgcc tggatgcgcg gcgtcgagat ggatcaggat tgccgcactt
      781 tcggacagcg cgctgccgtc gggcagcgcc agcgtcggta tctggccgat cggattgatc
      841 ttgagcagat cgcgaaaggc gtcgttagtc tcccacgacg ccgtttcgac catgcgatac
      901 ggcgcgcctg ccatcgtcag cgcggcctcg atcgtcgccg atccggagcc tttcttgccg
      961 tacagcgtat acatgcgtcc cccttttttt ctgccgcgga tcggcgatcg cgcgcacgac
     1021 ggaatcacgg gccggatcgc tcgcgctcac cggccgatga tcggatgccg ctatcgatac
     1081 ggtctattct gctcgtgcgc cgcgactagg cattctgccc gtctgccatc atcttttcca
     1141 ttcgcttttg catgtcggcc accgagacgt cttccttgtg cgtcgcgatc gtccacatca
     1201 agccgaacgg atcggtcaac gtgccgctgc ggtcgccata gaactgatcc ttgaccggat
     1261 tcttgacttt gccgcccgct gcaatcgcct gcgcgaaact cttgtcgacg tcgggcacgt
     1321 aaaacatcag gctgaccggt gacccgccgt atgactgtac gctgcgatgg cccatttcgg
     1381 gcgactcgtc ggccagcatg atattgccgc cggcgatctt gagctcggcg tggccgacct
     1441 tcccgtccgg gccggggaag cgcatcacct cggtcgcgcc gaacgccttc ttgtagtagt
     1501 cgatcgcgct ggccgcgtcg cggatgatga gataggaggt gacgccggcg tagccggcgg
     1561 gaattggctg gaccatgaga aatctcctcg agtattcaat atggttccgg cgaatgccgg
     1621 aaccggtctg ctgcctgcta cttcaacgcg gccgtgcgca acagtcccac cgcgatgccc
     1681 tcgatcgcca aatccgtgtg ggcaggattt acgacaatcg gttcaaactc cgagttctcc
     1741 gggtgcagca cgatttcgcg gccgcggcgt ctcaggcgct tgacggtcac ctcgtcgccg
     1801 gcagcgccgg acaggcgggc gacgacgatc tggcccgagc gcgcgtcggc agtccggtgc
     1861 acgaccagca aatcgccgtc gaggatgccg acatccttca tgctcatgcc gcggacgcgc
     1921 agaaggtagt cggcccgtgg cgagaacagt gcggcatcga cccggtagcg tgcctcgacg
     1981 ttctccgccg ccaggatcgg ctggccggcg gcgactcttc cgaccaacgg caacgtgccc
     2041 tggaccggaa ccccggcgat atccttcaac cgcagccccc gggacgagcc cggcaccagt
     2101 tcgagcgcac ccttcttggc cagcgcctgc agatggtcct cggccgagct ggcgctggaa
     2161 aatcccaggc ctgcggcgat ctcggcgcgc gtcggcggca tgcctctcga ctcgatgaag
     2221 ccgcggatcc aatcgagaat ctgttgctgg cgggcggtcg ggcgaggcgc gagcggcgtt
     2281 gcgggcgccg ccggactgcg gcgcaggttg gcaagcgggc ggtgtggcat cggcagatac
     2341 cgtatgtatg atcggtaccg atattataac gggtattcgc ctcggtcaag cggatgcgct
     2401 gactgcgccg acgagatatg tcgaacccgg tgctatcctt ctttcgttca ctgggtgacg
     2461 cccgggtaaa tgcctcggag gatgcgatgg acgttgggtc cgctgttcga cggctgctga
     2521 taacggcggg aacgaccgcg ctgtttgcga gcagcccctt cgcgatcgcc gatgcctata
     2581 ccgaccgcat agtcatgcgc ggggcgctcc cctatgaccg gtcttacgag cagctgagcc
     2641 cggagcaaaa ggccgtggtc cgggcgcaat acgatagcgt cggcccaaac gacgaaccgc
     2701 catacccgaa gtacggcctg agcgacgtgg ccgacgcagt ggcacggatg ccgcggcgcg
     2761 aaccgctcga gggagaggtg attcttacgg tgagcgtcga cgagcgtggt aacgccaaat
     2821 cggtgtccat ttacaagacc cccgacgcca agctgtccaa tgtggtcgcg gcgacgttga
     2881 cgcgaagcaa gttcaagccg ggactttgcg aacgccagcc atgcgcgatg gattacgtgt
     2941 tcaagtacta tttcaaaatc agcgcctgac tctctttcgg atgaa
//
LOCUS       DATANS010000456         1998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_778757, whole genome shotgun sequence.
ACCESSION   DATANS010000456 DATANS010000000
VERSION     DATANS010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778757"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..1037
                     /locus_tag="VHT22_06755"
     CDS             42..1037
                     /locus_tag="VHT22_06755"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3137290.1"
                     /translation="MHQPIKSPGEDLANGGLKLDRETEALLYWASSSTSTPLWHLSPE
                     AARNDYRRGLAKTEIVPPEVGETSDFDVPSDAGALRLRKYIPADPNIDAEAAILFVHG
                     GGCVIGDLETHDVFCRALCHDTRATVLSLDYRLAPEHPFPAAVEDTVAALEWLSHEAR
                     ALGLDPARIAVAGDSAGGGLAAVALHETKGKLAAPVRGQALIYPALDLRGRLPSRKEL
                     ADQFPIPRDMIDWFFDHYFGNAWPFTDPRAIPSLYEDCTGLPPALVITAGYDPLRDEG
                     AEYAETLAAAGVPVDYECCEGTVHGFMNMGRVLRRAHGKTRRRIAGWLAERLRKA"
     gene            1069..1813
                     /locus_tag="VHT22_06760"
                     /pseudo
     CDS             1069..1813
                     /locus_tag="VHT22_06760"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
BASE COUNT          335 a          642 c          649 g          372 t
ORIGIN      
        1 cgaagtgaag cagagagcgg gcggcggcgc cgagtgccaa tatgcaccag ccgattaaat
       61 ctcccggcga ggatttggcg aacgggggtc tcaagctcga ccgcgagacg gaagcgctgc
      121 tttattgggc gtcgtcctcg acttcgacgc cactctggca tctcagcccc gaggccgcgc
      181 gtaacgacta tcgccgcggc ctcgctaaaa ccgaaatcgt gccgccggaa gtcggagaga
      241 caagcgattt cgatgtaccg agtgatgccg gagcgttgag gctacggaaa tacattcccg
      301 ctgatcccaa tatcgatgcc gaagcagcaa tcctgttcgt gcatggcggc ggttgcgtga
      361 tcggcgatct cgagacgcat gacgtgttct gccgcgcgct ctgccacgac acgcgcgcga
      421 cggtactctc gctcgattac cggcttgcgc cagagcatcc gttccccgcg gcagtggagg
      481 acaccgtcgc cgcgctcgaa tggctgtcgc acgaagcgcg cgcgcttggc ctcgatcctg
      541 cgcgtattgc cgttgccggc gacagcgcgg gaggcggctt ggcggccgtc gcgctgcatg
      601 agaccaaggg caagcttgcc gcccctgtgc gcggccaggc tctgatctat cccgcgctcg
      661 acttgcgcgg ccggctgccc tcgcggaaag agctcgccga ccagtttccc atcccgcgtg
      721 atatgatcga ctggttcttc gatcactatt tcggcaacgc ctggccattc acggacccgc
      781 gcgctatccc gtcgctttat gaggattgca caggattgcc gccggccctc gtcattactg
      841 ctggctacga tcccctgcgc gacgagggcg ccgaatatgc cgagacgctc gcggccgctg
      901 gcgttcccgt cgattacgaa tgctgtgagg gaaccgtcca cgggttcatg aatatgggcc
      961 gcgtgctgcg ccgcgcgcac gggaagacac gccgccgtat cgcgggctgg ctggcggagc
     1021 gcctgcgcaa ggcatagaac gggaccggat agagggaggt ttttgtgggt ggacgccctg
     1081 tccgacgttc ttgccgccat caggctggat ggcgcggtct atatcaaagc cgagttcaag
     1141 gcgccgtggt gtgtcgagac gcaatacggc ctgcatacgg cggcgacccg cctgccgcgc
     1201 gcggaccaca tcgtattctt ccatcttctc actgacggct cgtgcaaagc ccggctcgtg
     1261 gacggcaacg aggatgtcga ggtcgcagcc ggcgatctgc tcctgtgata accgccatct
     1321 gctcggcagc gatcttatgc tgtcgcctgc accggtcggc tcagaggcga ttgaaggcct
     1381 gatcgagatg cgctggggcg gcgacggcga agcgacgcgg ttcatctgcg gccgtcttgc
     1441 ctgcgatcgt cgcgcctgcc gggcgctgct cggacctctg ccggcgatgc tacgcatacc
     1501 actcggcgac atctcgaaaa gcggctggct cggcgatctg cttcacattg gcgtgcagga
     1561 atcgctcgcc aagcggcctg gctcccagtc gttgctcgca aagctttcgg aacttgcttt
     1621 catcgaagcg ttgcgccgct atgcgcagtc gaggccgccc gatctcaaag gctggcttgc
     1681 gggattgcag gatccacatg tcggccgcgc actcgctcta ctgcatggcg atccgaaacg
     1741 cgcctggacg gtcgatgagc ccgtgcgatg gcattgtcac gctcggcgct tgcggagcgc
     1801 ttcaccgaga tgatcggttc gccgccgata cagtaattga cccagtggag gcttacgctt
     1861 gcggcgcagg cggcgctccg gtagcgacgg cattgcgcgc atcgccgaga gcagcggcta
     1921 tcactccgag gccgccttca cccgcgcatt caagcgcgag tttggcgtgc cgccgagcgc
     1981 gggtccagcc acgcagag
//
LOCUS       DATANS010000457         2328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_485714, whole genome shotgun sequence.
ACCESSION   DATANS010000457 DATANS010000000
VERSION     DATANS010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485714"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VHT22_06765"
     CDS             <1..911
                     /locus_tag="VHT22_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3137291.1"
                     /translation="TEAIVTIGSKEGIAHLTLATLERGDVVLVPNPSYPIHIYGPVIA
                     GADIRQVQLTPDVDFFAELDRTIKSSFPKPKMLIINFPANPTAQCVELPFFEKIIALA
                     REHGIYVVHDLAYADICFDGWKAPSIMQVPGAREVAVEFFTLSKSYNMAGWRIGFMVG
                     NQDLVHALSRIKGYHDYGTFTPIQVASIAALEGPQDCVAEIAMTYQKRRDVLVKGLHE
                     AGWKVDIPKASMYVWAAIPEAYRQLGSLEFTKQLLARAKVSVSPGIGFGEFGDGHVRF
                     AMIENEARTRQAIRGVKQMFREDGLA"
     gene            935..2263
                     /locus_tag="VHT22_06770"
     CDS             935..2263
                     /locus_tag="VHT22_06770"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX3137292.1"
                     /translation="MKPLQVGLLGMGTVGRGTWDVLRRNEEEIARRAGRPIRITHIAT
                     RTLSRAQSATRDVDGVRLDTDPQTVVDDRDIDIVVELIGGIEPAKAYVLRAIANGKHV
                     VTANKALLALHGNEIFAAAQKAGVMVAFEAAVAGGIPIIKALREGLTANRIEWIAGII
                     NGTSNFILSEMRATGATFAAALKDAQAKGYAETDPTFDVDGIDAAHKLTIMSAIAFGV
                     PMQFDKAYTEGISRLAREDIRYAEELGYRIKLLGITRRARSNGRDGIELRVHPTLVPH
                     KRLIANVEGAMNAVLVKGDAVGPTLYYGPGAGAEPTASAVIADLVDVTRMHTADPEHR
                     VPHLAFQPDQLSDTPVLAIGDVETSYYLRMRVLDRPGVLADITRILADREISIDAMIQ
                     KEPSEGAEQTDIILLTHRSVERGVVDAIERIESLPTVRGSVVRIRLEDLG"
BASE COUNT          448 a          751 c          757 g          372 t
ORIGIN      
        1 aaaccgaagc gatcgtcacg atcggctcga aggaagggat cgcccacctg acgctggcga
       61 cgctggagcg cggcgacgtc gtgctggttc cgaatccgag ctacccgatc cacatctacg
      121 gcccggtgat cgcgggcgcc gatatccgcc aggtccagtt gacgccggac gtcgacttct
      181 tcgccgagct cgatcgcacg atcaagtcga gctttcccaa gcccaaaatg ctgatcatca
      241 atttcccggc gaatccgacc gcgcaatgcg tcgagctgcc gttcttcgag aagatcatcg
      301 cgctggcccg cgagcatggc atctatgtgg tccatgatct ggcgtatgcc gacatctgct
      361 tcgacggctg gaaagcgccg tcgatcatgc aggtgcctgg agcgcgcgaa gtcgccgtcg
      421 agtttttcac cctgtccaag agctacaaca tggcgggctg gcgaatcggc ttcatggtcg
      481 gcaaccagga tctggttcac gcgctgtcgc ggatcaaggg ataccacgac tacggcacgt
      541 tcacgccgat ccaggttgcc tcgatcgcgg cgctcgaagg accgcaggat tgcgtcgcgg
      601 aaatcgcgat gacctaccag aagcggcgcg atgtgctggt caaggggctg cacgaggccg
      661 gatggaaggt cgacatcccg aaggcgtcga tgtacgtgtg ggcggcgatt cccgaggcgt
      721 atcggcagct cgggtcgctc gaattcacca agcagctgct ggcaagggcc aaggtgtcgg
      781 tatcgcccgg catcggcttc ggcgaattcg gcgacggtca cgtgcggttc gcgatgatag
      841 aaaatgaagc ccggacgaga caggcgatcc gaggcgtcaa acagatgttc cgcgaagacg
      901 gactggctta gggcgcgcaa tggccgatgc cgcgatgaag ccgctgcagg tgggattgct
      961 cggcatgggc acggtggggc gcggcacctg ggacgtcttg cggcgcaacg aagaagagat
     1021 cgcgcgccgc gccggccgtc cgattcggat cacccacatc gccacgcgca cactctcccg
     1081 tgcgcagtcg gcgacgcgcg acgtggacgg ggtgcggctc gacaccgatc cgcagaccgt
     1141 cgtcgacgac cgcgacatcg acatcgtcgt cgagctgatc ggcggcatcg agccggcaaa
     1201 ggcctatgtc ctgcgggcga tcgccaacgg caagcatgtc gtcaccgcga acaaggcact
     1261 gctggcttta cacggcaacg aaatcttcgc cgctgcgcaa aaggcaggcg tgatggtcgc
     1321 attcgaagcc gccgtcgccg gcggcatccc gatcatcaag gcgctgcgcg aaggcctgac
     1381 ggcgaaccgc atcgagtgga tcgccgggat catcaatggc accagcaact tcatcctgtc
     1441 ggagatgcgc gctaccggtg cgacgttcgc cgccgcgctc aaggacgccc aggcgaaggg
     1501 ctacgccgag accgatccga ccttcgacgt cgacgggatc gatgccgcgc acaagctgac
     1561 gatcatgtcg gcgatcgcat tcggcgtgcc gatgcaattc gacaaagcat acaccgaagg
     1621 catctcgcgg ctcgcgcgcg aggacatacg ctacgccgag gagctcggct accggatcaa
     1681 gctgctcggc atcacgcgcc gcgccagatc gaacggccgc gacggcatcg aattgcgcgt
     1741 gcatccgacg ctggtcccgc acaagcgtct gatcgccaac gtcgagggcg cgatgaacgc
     1801 ggtgctggtc aagggcgatg ccgtcgggcc gacgctctac tacggccccg gcgccggcgc
     1861 cgagccgacg gcgagcgcgg tcatcgccga cctggtcgac gtcacgcgca tgcacacggc
     1921 cgatcccgag caccgcgtgc cgcatctggc gttccagccc gaccagcttt ccgacacgcc
     1981 ggtgctggca atcggcgacg tcgagaccag ctattacctg cgcatgcggg tgctggaccg
     2041 cccgggcgtg ctggccgaca tcacgcgcat cctcgccgac cgcgaaatct cgatcgacgc
     2101 gatgatccag aaggagccgt ccgaaggcgc cgagcagacc gacatcatcc tgctgacgca
     2161 tcgctcggtc gaacgcggtg tcgtcgacgc gatcgagcgg atcgaaagcc ttcccacggt
     2221 gcgcggtagc gtcgtgcgca tccgcctcga agatctcggc tgatcgatgt tagtcacgtt
     2281 gcgcgagcca agcattccgg cgtcgagcgg agcacattga tgcgttac
//
LOCUS       DATANS010000458         1972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_696671, whole genome shotgun sequence.
ACCESSION   DATANS010000458 DATANS010000000
VERSION     DATANS010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696671"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..403
                     /gene="rpsJ"
                     /locus_tag="VHT22_06775"
     CDS             92..403
                     /gene="rpsJ"
                     /locus_tag="VHT22_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX3137293.1"
                     /translation="MQNQRIRIRLKAFDYKLIDQSALEIVDTAKRSGAVVRGPVPLPT
                     KIERYNVLRSPHVNKTSRDQLEIRTHLRLMDIIDPTDKTVDQLMKLDLPAGVDVEIKL
                     Q"
     gene            461..1117
                     /gene="rplC"
                     /locus_tag="VHT22_06780"
     CDS             461..1117
                     /gene="rplC"
                     /locus_tag="VHT22_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041929744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX3137294.1"
                     /translation="MSLGLVGRKVGMTRIFTDDGAAVPVTVLDVANNRITQIKTQEAD
                     GYTAVQLTYGKRRASRVSKPQAGHLAKAGVEAGELLKEFPVSAEQLASLKLGETVSVE
                     TFAVGQLVDVSGTTKGRGFSGAIRRHHFSSNRASHGNSRSHNSPGSISMAQDPGRVFP
                     GKRMAGQYGNVNRTVQTLKVVRVDAARGLLLVKGAVPGADGGHIVVRPAAKAPLKKGA
                     "
     gene            1120..1743
                     /gene="rplD"
                     /locus_tag="VHT22_06785"
     CDS             1120..1743
                     /gene="rplD"
                     /locus_tag="VHT22_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX3137295.1"
                     /translation="MDLKLIDDSGKSAATHAGSDALFGREYNEALIHQLVTAYQANAR
                     SGNRKQKGRADINKSHKKPWAQKGTGRARAGQANSPLWRGGGKIFPNSPDENFSQKIN
                     RKMFRAGIAAILSQLVRENRLSVVEQFALDAPKTKLFAQKIKGFGLTGSVLVVTDKFD
                     DNVFLSSRNLPGVLVLEAREVDPVSLVRFDNVLLTKAAVAQFEEILG"
     gene            1740..>1972
                     /locus_tag="VHT22_06790"
     CDS             1740..>1972
                     /locus_tag="VHT22_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137296.1"
                     /translation="MSPTKATATTGKSAKAAAKTAAKKAAKTSAKTAAKATDKTAAKA
                     TDRKTAKTSAKTPAKTPGPKYKPERLLTLLLAPT"
BASE COUNT          426 a          631 c          627 g          288 t
ORIGIN      
        1 gaataactcg gcgcgacgtg gttgcagttt tcgcaggaca gtgaaatgaa acaccgcgcg
       61 cgtcacgagg cgcggtgcaa agggtgaggc gatgcagaac cagagaatca gaattcggct
      121 gaaggcgttc gattacaagc tgatcgacca gtcggcgctg gaaatcgtcg acaccgccaa
      181 gcgctcgggc gccgtggttc gtggtccggt gccgctgccg accaagatcg agcgctacaa
      241 cgtcctgcgc tcgccgcacg tcaacaagac gtcgcgcgac cagctcgaga ttcgtacgca
      301 tctgcggctg atggacatca tcgatccgac ggacaagacc gtcgaccagc tgatgaagct
      361 cgatctgccg gcgggagtcg acgtggagat aaagcttcag taacgcaact taatgtcgct
      421 gccggtcaat cgtaatcggc ggctttgaag gaagacgaaa atgagtctag gactagtcgg
      481 ccgcaaagtc ggcatgacgc ggatcttcac cgacgacggc gccgccgttc cggtgacggt
      541 gctcgacgtc gccaacaatc gcatcaccca gatcaagacg caggaagccg acggctacac
      601 cgccgtccag ctcacctacg gcaagcgtcg cgccagccgc gtcagcaagc cgcaggccgg
      661 acatctggcc aaggcgggcg tcgaggcggg cgagctcctg aaggagtttc cggtttccgc
      721 cgagcagctc gcgagcctca agctcggcga gacggtcagc gtcgagacct ttgccgtcgg
      781 gcagctggtc gacgtttccg gcacgaccaa gggccgcggc ttttccggcg cgatccggcg
      841 tcaccatttc agctcgaatc gcgcctcgca cggcaattcg cgctcgcaca attcgcccgg
      901 ctcgatcagc atggcgcagg atccgggccg cgtgtttccg ggtaagcgca tggccggcca
      961 gtacggcaac gtcaaccgca ccgtgcagac attgaaggtc gtccgggtcg atgcggcccg
     1021 cggcctgctg ctggtcaagg gcgcggttcc cggcgccgac ggcggccata tcgtcgtccg
     1081 tccggccgca aaggccccgc tgaagaaggg ggcctgaaca tggatctgaa actgatcgac
     1141 gacagcggaa agtccgcggc gacgcacgcc ggctccgacg ccctgttcgg ccgcgagtac
     1201 aacgaagcgc tgattcatca gctcgtgacc gcataccagg ccaacgcacg cagcggcaat
     1261 cgcaagcaga agggccgcgc cgacatcaac aagtcgcaca agaaaccctg ggcgcagaaa
     1321 ggcaccggcc gcgcgcgcgc cggccaggca aacagtccgc tgtggcgcgg cggcggcaag
     1381 atcttcccca actcgcccga cgagaacttc tcgcagaaga tcaaccgcaa aatgtttcgc
     1441 gccggcatcg ccgcgatact ttcgcaactg gttcgcgaga accgcctttc ggtggtcgag
     1501 cagtttgccc tcgatgcgcc gaagacgaag ctgttcgcgc aaaagatcaa gggcttcggc
     1561 ctgaccggca gcgtgctggt cgtcaccgac aagttcgacg acaacgtatt cctgtcgtcg
     1621 cgcaacctgc ccggcgtgtt ggtgctcgag gcgcgggaag tcgatccggt cagcctggtg
     1681 cgcttcgaca atgtgctgct gaccaaggcc gcggtcgcgc aattcgagga gattctggga
     1741 tgagcccgac caaggcaacg gccacaaccg ggaagtccgc caaggcggcc gcaaaaaccg
     1801 cggcgaaaaa ggctgcgaag acctccgcga aaacggcggc gaaggcgacc gataagaccg
     1861 cggcgaaggc gaccgacagg aagaccgcga agacgtcggc caagacgccg gcgaaaacgc
     1921 ccggaccgaa atacaagccc gaacggcttc tcacgctgct gctcgcaccg ac
//
LOCUS       DATANS010000459         1925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_444405, whole genome shotgun sequence.
ACCESSION   DATANS010000459 DATANS010000000
VERSION     DATANS010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444405"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1235)
                     /locus_tag="VHT22_06795"
     CDS             complement(153..1235)
                     /locus_tag="VHT22_06795"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3137297.1"
                     /translation="MERKQIGLAVVGSGRIGTLRAKLASSHPAVNFIAVSDTNLGAAQ
                     KLAQDVGAQFASANNDEIIARPEVTAVIVSTSEGEHLAPVLKAIELGKAVLVEKPIAL
                     NIADADRIIAAARKANVDLRVGYSRRFKDRYLIAKEQIIQGRVGKIIGGAARVFNSRS
                     QALAMMKRNPEATPVVDALTYYVDLMNWLLEGNAPVEVFARGQTGVLKAAGHNVDDVN
                     WSIVTYADGAVVNLGVSYALPEKYPALGHAARVEILGTEGVIILDDDHTDQLMYSNKG
                     VPHVYLPDHNVNMVFLSSGTPGDWALGDFWGPIANETRAWLDHLATGKACVHATAQDA
                     RCNLEVTLAIQLAAASGNTVKLPLAG"
     gene            complement(1246..>1925)
                     /locus_tag="VHT22_06800"
     CDS             complement(1246..>1925)
                     /locus_tag="VHT22_06800"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX3137298.1"
                     /translation="INQSLYSQMPYDSNTAFAPITNMVKTTNVLIVTPSLPVKSVQDL
                     VAYGKANPGKLTYASAGAGGTIHLSGELFKSMTGITMEHIAYKSSPLAHIDMIAGQVH
                     AMFDAMPTALPQIKANRLRAIAVTTAKRSPQLPELPTIAEAGVPGFEAAGWFGYAAPA
                     RTPKEIVATLNREVVRILALPDVRERLIAQGAEPVGDTPEQFAQYIKAEAAKWGKVIK
                     TLNLKIE"
BASE COUNT          320 a          613 c          597 g          395 t
ORIGIN      
        1 gtgccgccgc cgggcggata agcgacgatc atgcggatgg gtttggttgg ataactttgc
       61 gcaagtgcgc ccgttgccgg caccgcaatc gcacaacaca aaacgaatcg caccgcttta
      121 ctcatcattc atcactcatc atttatcatt ggtcaccctg ccagcggcag cttgacggtg
      181 ttgccgctcg ccgccgccag ctggatcgcc agcgtcactt cgagattgca gcgcgcgtcc
      241 tgcgccgtcg cgtgcacaca ggccttgccc gtcgcgagat ggtcgagcca cgcgcgcgtc
      301 tcgttcgcga tcggccccca gaagtcgccg agcgcccagt cgccgggcgt gccgctcgac
      361 aggaacacca tgttgacgtt gtgatcgggc aggtagacgt gcggcacgcc tttgttgctg
      421 tacatcagct gatcggtgtg atcgtcatcg aggattatca cgccctcggt gccgagaatc
      481 tcgacgcgcg cggcgtgacc caacgcggga tatttttccg gcagcgcgta gctcacccct
      541 agattgacta ccgcaccgtc cgcgtaggtg acgatcgacc agttgacgtc gtcaacgttg
      601 tggccggcgg ccttcaatac gcccgtttgt ccgcgcgcga acacttcgac cggcgcattg
      661 ccttcgagca gccagttcat gaggtcgacg taatacgtga gcgcgtccac caccggcgtg
      721 gcctcgggat tgcgcttcat catggccagc gcctgcgagc gcgaattgaa cacgcgcgcc
      781 gcgccgccga tgatcttgcc cacgcggccc tggatgatct gctccttcgc gatcaggtag
      841 cggtctttga agcggcggct gtagccgacg cgcagatcga cattagcttt cctcgccgcc
      901 gcgatgatgc gatccgcatc cgcgatattg agagcgatcg gcttctcgac gagcaccgct
      961 ttgccgagct cgatcgcctt cagcaccggc gcgagatgct cgccctcgct ggtcgacacg
     1021 atgaccgcgg tgacttcggg ccgcgcgatg atctcgtcgt tgttcgccga ggcgaactgc
     1081 gcgccgacgt cctgcgcgag tttttgcgca gcgccgagat ttgtgtccga caccgcaatg
     1141 aagttgaccg ccgggtgaga cgaggcgagc ttcgcccgca gcgtcccgat gcggcccgaa
     1201 ccaacgacgg cgagcccgat ttgcttgcgt tcgatgaccg gctccttact cgattttcag
     1261 attcagcgtc ttgatgacct tgccccactt cgcggcctcg gccttgatgt actgcgcgaa
     1321 ttgttccggc gtatcgccga ccggttccgc accctgcgcg ataagacgct ctctaacgtc
     1381 gggcagtgcg aggatgcgca cgacctcccg gttgagtgta gcgacgattt ctttcggcgt
     1441 gcgcgcgggc gccgcgtaac cgaaccagcc ggcggcctcg aaccccggaa cgccggcctc
     1501 cgcaatcgtc ggcagctcgg gtagttgcgg cgagcgtttc gcggtggtca ccgcgatggc
     1561 gcgcaaacgg ttggccttga tctgcggtaa tgcggtcggc atcgcgtcga acatcgcgtg
     1621 cacctggccg gcgatcatgt cgatatgcgc gagcgggctc gatttgtacg cgatgtgctc
     1681 catggtgatg ccggtcatcg acttgaacaa ctcgccggag aggtggatgg tgccgcccgc
     1741 gcccgccgac gcgtaagtga gcttgcccgg attggctttg ccgtaagcga cgagatcctg
     1801 caccgatttc acaggcaacg aaggcgtgac gatcagcacg ttggtcgtct tgaccatgtt
     1861 cgtgatcggc gcaaacgcgg tattgctgtc gtacggcatc tgtgaataca ggctttggtt
     1921 tatcg
//
LOCUS       DATANS010000460         2655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_738548, whole genome shotgun sequence.
ACCESSION   DATANS010000460 DATANS010000000
VERSION     DATANS010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738548"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VHT22_06805"
     CDS             complement(<1..738)
                     /locus_tag="VHT22_06805"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3137299.1"
                     /translation="MNQEMQSFVASDGLKLSYGIDDYTDPWRTPDTLVMVHAAMGSSR
                     RFYAWVPHLARDYRVVRLDQRGHGQSGIPAPEQMTFKRLGKDVVELLDHLGVQSAHLA
                     GSSAGGIVSQSVAIDYPKRVKSLASFAAPPGMKMGRQDYNGWLAKIGSKGLATFLRET
                     IADRMDLSQVEPGFVDWFIEESARNKVESLARFVPTVAVIDHTDELHKIQCPTLIVAP
                     GGDPIHLIDEYELLREKIKRCEFVVYDK"
     gene            1351..2202
                     /locus_tag="VHT22_06810"
     CDS             1351..2202
                     /locus_tag="VHT22_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3137300.1"
                     /translation="MRFTSVIMLLAGLACTGHVGAADTLAKIKANGEIRLGYFSASAP
                     FSFAGTDKSPQGYSVELCQRIAAGVQRELGLKQLKTTWVELGLGDRIEAVRSGRIDIE
                     CGTTSWSFSRQQLVDFSLMTFIDGASLLATIDSGIKRITDAGGKRISVIRGTTTERAL
                     VAALAKEKVQAELVRVDTREQGFAMLTEGRVDAFASDRFLLLNAFTSLRSNKPLRLAD
                     EDFSVEPYALALPRDDAHFRIAVNRALAEVFRSGEISGIYDRWFGQFGQPSLLLSALY
                     YLQQVPE"
     gene            2208..2639
                     /locus_tag="VHT22_06815"
     CDS             2208..2639
                     /locus_tag="VHT22_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137301.1"
                     /translation="MARAYFAAALFALAALPDEAPAQPAGSTPIPDSVVMRCRQCGVI
                     NSIRETQQQREGQTPGLGPDSPVGLVLYIPMGPGRKQGDAFAGSVGNREWQNRISSTR
                     YEYTVRMDDGDFRLVSKDGVSDLQVGDRVRLEGNRIERWGS"
BASE COUNT          481 a          852 c          831 g          491 t
ORIGIN      
        1 cttatcgtag acaacgaatt cgcagcgctt gatcttctcg cgcagcagtt cgtattcatc
       61 gatcaggtgg atcgggtcgc cgcccggcgc gacaataagc gtcgggcact ggattttgtg
      121 cagttcgtcc gtatggtcga tgacggccac cgtcggcacg aagcgtgcga gcgattcgac
      181 tttgttgcgc gccgattcct cgatgaacca gtcgacgaag cccggctcga cctggctcaa
      241 atccatgcgg tcggcaatcg tctcgcgcag gaacgtggcg agccctttgg agccgatctt
      301 cgcgagccat ccgttgtaat cctggcggcc cattttcatg ccgggcggcg cggcgaagct
      361 cgcgagcgac ttcacgcgct tgggataatc gatcgcgacg ctttgcgaca cgatgccgcc
      421 cgcggacgag cccgccagat gggccgactg aacaccgaga tgatcgagca gctcgacgac
      481 gtccttgccc agccgcttga acgtcatctg ttccggcgcc ggtatgcccg actgcccgtg
      541 gccgcgctga tcgaggcgca cgacgcgata gtcgcgcgcg aggtgcggca cccacgcgta
      601 gaagcggcgc gagctgccca tcgcggcgtg gaccatcacc agcgtatccg gcgtgcgcca
      661 cgggtcggtg taatcgtcga ttccatagct caacttcaag ccgtcgctcg cgacaaagct
      721 ttgcatttcc tggttcatcg gctcatgcct ggcgggaatc gaaacggagc aatacgttaa
      781 catattcccc tgtcgggaat ttcgcatccc gccccatgcg gcgcatgcgc gctcgcgatt
      841 ttctgcatcg gcaattcgac ttcgatacgc cgcattgatc ctgatcaaag agccgtgccg
      901 ccggccgcgc acaatgaacg cacgttaaat caccggcaag gatttcatca tgtggcgttt
      961 taattatctg cccggctggg cgaccccgaa tgcgttgcgc agcggctacg ccgcgctcgc
     1021 caacgccttg catcatcgtg acacgcgcgg ccggtgtctg cggcgccggc agggccggtg
     1081 cccgggtctc gaatactgcg cggactgtgc gtggaaggaa ggcacgtcgc gcgaacgcgg
     1141 ctagcgcatt gactcccgcc ggctgcgtga tctcgcagcg aaaatccgcg gcacggcggc
     1201 ggctggcgaa tcattgtcgg ccaggattgt catcacttcg cgcaagcgga gcctattata
     1261 atggctgggc gtggtcgtag aatcccgcgc cgacaaaaat tgcggtaacc tcgcaccgca
     1321 ggtcgcgttc agacaaggga gatttccgca atgagattca cgtccgtcat catgctgctc
     1381 gccggacttg catgcaccgg tcacgtcggc gcagccgata cgctcgccaa aatcaaggcc
     1441 aacggcgaaa tccggcttgg ctatttctcg gcatccgcgc cgttctcgtt cgcaggcacg
     1501 gacaagtcgc cgcaaggcta ttcagtcgag ctgtgccagc gcattgccgc cggcgtccaa
     1561 cgcgagctcg ggctcaagca actgaagacg acgtgggtcg agctcgggct cggcgaccgc
     1621 atcgaagccg tgcgcagcgg acgcatcgat atcgaatgcg gcaccacgag ctggtcgttc
     1681 tcgcgccagc agctggtcga cttcagcctc atgacgttca tcgacggcgc gagcctgctg
     1741 gcgacaatcg actcgggcat caagcgcatc accgacgcag gcggcaagcg catttcggtg
     1801 atccgcggca cgacgaccga aagagcgctc gtggcggcgc ttgccaagga aaaagtgcag
     1861 gcggagctcg tcagagtgga cacgcgcgaa cagggcttcg caatgctgac tgagggccgc
     1921 gtggacgctt ttgcatccga ccgctttttg ctgctcaacg cgttcacgtc gcttcgctcg
     1981 aacaagccgc tgcggctcgc ggacgaagat ttttcggtcg agccttacgc gctcgcactg
     2041 ccgcgcgacg acgcgcactt ccgcatagcg gtcaatcgtg cgcttgccga agtattccgc
     2101 tcgggtgaga tttcggggat atacgaccgc tggtttggcc agttcgggca gccgagcctg
     2161 ctgctctccg cgctctacta tctgcagcag gttccggagt aagcgccgtg gcgcgtgctt
     2221 actttgccgc tgcgctcttc gcgctcgcgg ccctgccgga cgaggcgccg gcgcagccgg
     2281 cgggcagcac cccgataccc gacagcgtcg tcatgcgctg ccggcagtgc ggcgttatca
     2341 attcaatccg cgaaacccaa cagcagcgcg agggccagac gcccggactc ggtcccgatt
     2401 cgccggtcgg gttggtgctg tatatcccga tgggccccgg acgcaaacag ggcgatgcat
     2461 tcgcgggctc ggtcggcaac cgcgagtggc agaaccgcat cagcagcacg cgctacgaat
     2521 atacggtacg catggacgac ggcgacttcc gcctcgtgag caaggacggc gtttccgacc
     2581 tgcaggtcgg cgaccgcgtg aggctcgagg gcaaccgcat cgagcgctgg ggttcctagc
     2641 tccgctagtc ctgac
//
LOCUS       DATANS010000461         4574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_232011, whole genome shotgun sequence.
ACCESSION   DATANS010000461 DATANS010000000
VERSION     DATANS010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232011"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..502)
                     /locus_tag="VHT22_06820"
     CDS             complement(59..502)
                     /locus_tag="VHT22_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX3137302.1"
                     /translation="MACIAPIAHAALSDRASDAGCSSKPTLVEGSMYKCTTRTGNIAY
                     FNVPGAVASTTRSPTPAGFPKVDAGTQKGRDILRNKVLTDELATEEKLLAEARAAYGD
                     GTPPPLPDEKANAQKYADRLARLRQTVALHEKNIEALKRELAASR"
     gene            complement(631..2046)
                     /gene="glnA"
                     /locus_tag="VHT22_06825"
     CDS             complement(631..2046)
                     /gene="glnA"
                     /locus_tag="VHT22_06825"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX3137303.1"
                     /translation="MAASADVLKMIKDKDVKFVDLRFTDTRGKEQHVQVPAKQFTQDK
                     FDSGHAFDGSSIAGWKGIEASDMLLMPDADSVRIDPFTEEITLNVTCDVVEPTDGKGY
                     DRDPRSLAKRAEAYLKSTGLGDTAYFGPEPEFFIFDSVTWHVDMSGCAVKIESEEAPW
                     STGRTDFEGGNSAHRAPVKGGYFPVPPVDSLQDIRNAISLSLEEQGVEVEVFHHEVAA
                     AGQCEIGTKFNSLVKRADWMQILKYTVHMVAHTYGKTATFMPKPIVGDNGSGMHVHQS
                     VWKGGQNLFAGNGYAGLSEFALFYIGGVIKHAKSLNAITNPGTNSYKRLVPGFEAPIN
                     LAYSARNRSAACRIPYVSNPKGRRVEVRFPDPTANPYLCFAALLMAGLDGVQNKIHPG
                     DPIDKNLYDLPPEVAAKVPHPCASLDEALDNLDRDREYLTRGGVFSNDMIDAYIALKM
                     EEVTRFRMTTHPIEFDMYYSS"
     gene            2397..2855
                     /locus_tag="VHT22_06830"
     CDS             2397..2855
                     /locus_tag="VHT22_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX3137304.1"
                     /translation="MSAAADSVLTAGHRRAADASLPYFGAVTPAEAMALLQSDPAARL
                     IDVRTRAEWDYVGRVPGSVLIEWTTYPDGTRNPQFVDELRRAVGETGAPVLFLCRSGH
                     RSDSAARAATAAGYTRAFNVLEGFEGNKDADGHRGNLGGWRKAGLPWVQG"
     gene            complement(2846..3694)
                     /locus_tag="VHT22_06835"
     CDS             complement(2846..3694)
                     /locus_tag="VHT22_06835"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HEX3137305.1"
                     /translation="MAEEKYTRCPGCKTIFRVSSQQLAMREGQVRCGHCRTVFDGELA
                     LVSLAPRPRGPDDEAQLSEAELGPPTVTLRNAQALSPVDEDTHDRREPAAGDRANPAA
                     SIDPELAYASRFSASEARQRLRLPAWAYGLLIPVLVIVLGAQALFHFRDAIAAHWPQT
                     KPALIRLCALADCQLKPLQDISGLSIEASDLQADPAHKGLLILSATIRNRAAHALGYP
                     YLELTLSNAQDQIVVRRAFAPGEYLSGAANLDGGIAGNAELTVKLFIDASTTSQAGYQ
                     VYLFYP"
     gene            complement(3687..>4574)
                     /gene="prmA"
                     /locus_tag="VHT22_06840"
     CDS             complement(3687..>4574)
                     /gene="prmA"
                     /locus_tag="VHT22_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX3137306.1"
                     /translation="PAATADAWSDAMLAAGAASVDAADAQAGTGDETPRYGEPGDAGG
                     LWSLCRLTALFAADADVEDSVRRAAAALGLATPTFTLETLSDQDWVRRTQAQFGPLQI
                     ADRLWVIPSWCEPVDPQAINLELDPGLAFGTGSHPTTRLCLRWLAAHLETGQSVLDYG
                     CGSGILAIAAAKLGAAAVTGTDVDPQAIEASRANAARNGVHAQFMLPDSLAPDKVDVV
                     VANILANPLRLLAPVLAARVRDGGAIVLSGILEDQVAVVSEAYRRWFKLDAWGTDDGW
                     VALTGRRDMRAMRSRRDRG"
BASE COUNT          762 a         1491 c         1533 g          788 t
ORIGIN      
        1 atagtagcaa gttccgcgcc cgcgctggcc gagtgggctc gggctcggcg acactcggtc
       61 accgtgacgc agccagctct cttttcaaag cctcgatgtt cttctcgtgc agcgccacgg
      121 tctggcgaag gcgagcgagc cgatcggcgt acttctgcgc gttggccttt tcatcgggca
      181 gcggcggcgg cgtgccatcg ccgtacgctg ctcgcgcttc agccagcagc ttttcctcgg
      241 tagcaagctc gtcggtcagg accttgttgc gcagaatgtc gcggcccttt tgcgttccgg
      301 cgtcgacctt cgggaaccct gcaggcgtcg gcgacctggt cgtcgaagcg accgcgcccg
      361 gaacgttgaa gtaagcaatg tttcccgtgc gtgtcgtgca cttgtacatg ctgccttcga
      421 ccagcgtcgg cttggacgag cagccggcat cgctcgcgcg atcggaaagc gcggcgtgcg
      481 cgatcggggc gatgcaggcc aacgcggagc agcagatagc ggcgacgcgt gcgagcgcag
      541 gggggaacga gcaccgggtc atcggcgcag tgtagcaaaa accgagggcg ggccgccgcc
      601 ccgcccccgt ccagccggtc tagtccgctt tcaggagctg taatacatgt cgaattcgat
      661 cggatgggtg gtcatgcgga aacgcgtgac ctcctccatc ttgagcgcga tgtaagcgtc
      721 gatcatgtcg ttggaaaaca cgccgccgcg cgtcaggtac tcgcggtcgc ggtcgaggtt
      781 gtcgagcgct tcgtcgagcg acgcacaagg atgcgggacc ttggcggcga cttccggcgg
      841 cagatcgtag aggttcttgt cgatcgggtc gcccggatgg atcttgttct gcacgccgtc
      901 gagccccgcc atcagcagcg cggcgaagca gaggtacgga ttcgcggtcg gatcgggaaa
      961 gcgcacttcg actcgccgcc ccttcggatt gctgacgtac ggaatccgac acgctgccga
     1021 gcggttgcgc gccgaatacg caaggttgat cggcgcctcg aatccgggca ccagccgctt
     1081 atacgaattg gtgccgggat tggtgatcgc gttcagcgac ttcgcgtgct tgatcacgcc
     1141 gccgatgtag aacagcgcga actcggacaa tccggcgtag ccattgccgg caaacaaatt
     1201 ctggccgccc ttccacaccg actgatgcac atgcatgccc gagccgttgt cgccgacgat
     1261 gggcttcggc atgaacgtcg ccgtcttgcc gtaggtgtgg gcgaccatgt gcaccgtgta
     1321 cttgagaatc tgcatccagt cagcgcgctt gaccaggctg ttgaacttgg tgccgatttc
     1381 acactggccc gcggcggcga cttcgtggtg gaacacctcg acctcgacgc cctgctcctc
     1441 gagcgaaagc gagatcgcgt tgcggatgtc ctgcagcgag tcgaccggcg ggaccggaaa
     1501 atagccgccc ttgaccggcg cgcgatgcgc cgagtttccg ccttcgaaat cggtccttcc
     1561 cgtcgaccac ggcgcttctt ccgactcgat cttgaccgcg caacccgaca tgtccacgtg
     1621 ccaggtcacc gagtcgaaaa tgaagaattc cggctcggga ccgaaatatg cggtgtcacc
     1681 cagcccggtc gacttcagat acgcctcggc gcgcttggcc agcgagcgcg gatcgcgatc
     1741 gtagcccttg ccatcggtgg gctcgacgac gtcgcaagtg acgttcagcg tgatctcttc
     1801 agtgaacgga tcgatgcgca cgctgtcggc atcgggcatc aacagcatgt ccgaggcttc
     1861 gatccctttc cagccggcga tcgaggagcc gtcgaacgcg tggccgctgt cgaacttgtc
     1921 ctgcgtgaat tgcttcgccg ggacctgcac atgctgctcc ttgccgcgcg tgtcggtaaa
     1981 gcgaagatca acgaacttga cgtccttgtc cttgatcatt ttcagtacgt cggcggatgc
     2041 agccatgggt tctccttgga atgggcgaag cgattgaatt tgctgcgccg aagcggcgtg
     2101 tttcgcagcg catcaaaaag cagaaactat gccataagca gccgcccttg gcaccgggcg
     2161 gaacaagggt ttgcggggct ggaccgtccg gtcgccggcg tcgccgcacc aaatcgtttc
     2221 tcgccggggc tcgcgtgcca cggtatggtg caacgcgcgc cgcggtggtt cgctccggtc
     2281 agaagggacc gaccgcgcta tacttttcgg atgcgccaga tcacttcccc ctgccccgag
     2341 cgcggggccc gcggttgccg gcagacacgc cgcgtcgcgc ccggcgagcg ctcccgatga
     2401 gcgccgccgc cgactcggtg ctgacggccg ggcatcgtcg tgcggccgat gcctcgttgc
     2461 cctatttcgg tgccgtgacg ccggccgagg cgatggcatt gctccagtcg gaccccgcgg
     2521 cgcggctgat cgacgtgcgc acgcgcgcgg aatgggacta cgtcggccgt gtcccgggca
     2581 gcgtgctgat cgaatggact acctatcccg acggaacgcg caatccccag ttcgtcgatg
     2641 agctgcgccg ggcggtcggc gaaaccggcg cgccggtcct gtttctgtgc cgcagcggcc
     2701 atcgttcgga cagcgccgcg cgcgcggcga cagcggccgg atacacgcgc gcgttcaatg
     2761 tattggaagg attcgagggc aacaaggacg ccgacggaca ccgcggaaac ctgggcggct
     2821 ggcgcaaggc cggcctgcct tgggttcagg gatagaacag gtagacctgg tagccggctt
     2881 gcgaggtcgt gctcgcgtcg atgaacagct tgacggtcaa ctcggcgttg ccggcgatgc
     2941 cgccgtcgag gttcgcggcg ccgctcaagt attcgccggg cgcaaacgcc cggcgaacca
     3001 cgatctgatc ctgcgcattg gacagggtca gctccagata cgggtagccc agcgcatgcg
     3061 cggcacggtt gcgtatcgtt gccgacagaa tcagcaagcc cttgtgcgca ggatccgcct
     3121 gcaggtcgga ggcctcgatc gacagcccgg agatgtcctg cagcggcttc aactggcaat
     3181 cggcgagggc gcaaagccgg atcagcgcgg gcttggtctg cggccagtga gcggcgatgg
     3241 catcgcggaa atggaacagg gcctgcgcgc cgaggacgat caccagcacc ggaatcagca
     3301 ggccatacgc ccaggccggg agccgcaggc gctgccgcgc ttcggacgcc gagaagcgcg
     3361 aggcgtaggc aagctcggga tcgatcgagg ccgctggatt cgcacggtcg cccgccgccg
     3421 gctcgcggcg atcgtgggta tcctcgtcga cgggtgagag cgcctgggcg ttgcgcaagg
     3481 tcaccgtcgg cggcccgagc tccgcttcgg agagttgcgc ctcgtcgtcg ggcccacgcg
     3541 ggcggggtgc cagcgaaacc aacgcgagct cgccgtcgaa taccgtgcgg caatgcccgc
     3601 agcggacctg cccttcgcgc atcgcgagct gttgcgagct aacgcggaaa atcgtcttgc
     3661 agccggggca gcgcgtgtat ttttcctcag ccacgatcac gcctcgacct catcgccctc
     3721 atgtcccgcc gaccggtcaa tgccacccat ccgtcgtcgg tcccccatgc gtcgagttta
     3781 aaccatcggc gatacgcttc gctgacgaca gcgacctggt cttcgagtat gcccgacaac
     3841 acgatcgcac cgccgtcccg gacgcgcgcc gccagcaccg gcgcgagcag tcgcagcgga
     3901 tttgcaagga tattggcgac gacgacgtcg accttgtccg gcgcaagcga atcgggaagc
     3961 atgaactgcg cgtggacgcc attgcgtgcg gcgttggcgc ggctggcttc gatcgcctgc
     4021 gggtcgacgt cggttccggt aaccgccgcc gcgccgagct tggcggcggc gatggcgaga
     4081 atgcccgagc cgcaaccgta atcgagcacc gactgtcccg tctcgagatg cgcggcgagc
     4141 cagcgcaggc agagccgcgt cgtcgggtga ctgccggtgc cgaacgccag gccggggtcg
     4201 agctcgagat tgatcgcctg cggatcgacc ggctcgcacc acgagggtat gacccacagc
     4261 cgatcggcaa tctgcagcgg cccgaactgc gcctgtgtgc gtcgcaccca atcctggtcg
     4321 gacaaggttt ccagcgtgaa tgtcggcgtc gcgagaccga gcgccgccgc ggcgcggcga
     4381 acgctgtctt cgacatcggc gtctgcggcg aacagcgcgg tcaaccggca cagcgaccac
     4441 agaccgccgg catctcccgg ctcgccgtag cgcggcgtct cgtcgccggt gccggcctgc
     4501 gcatcggcgg cgtcgaccga tgccgctccg gcggcgagca tcgcgtcgga ccaggcgtcg
     4561 gccgtcgcgg cggg
//
LOCUS       DATANS010000462         1979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_193162, whole genome shotgun sequence.
ACCESSION   DATANS010000462 DATANS010000000
VERSION     DATANS010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193162"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="VHT22_06845"
     CDS             complement(<1..1039)
                     /locus_tag="VHT22_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEX3137307.1"
                     /translation="MSTALVFALVCAVAALVYGGWSITWILQKPAGNARMQEIAAAVQ
                     QGAQAYLNRQYATIAVVGIILLVIIGVFLSWTTAIGFAVGAVLSGLAGFIGMNVSVRS
                     NVRTAEAARTGLNEALAVAFRGGAITGLLVVGLGLLGVAGFYWFLVQSAHYAPNATKE
                     GLHGIIEPLVGLAFGGSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAV
                     IADNVGDNVGDCAGMAADLFETYAVTIVATMLLGALLLPAYAEAAVTYPLALGGFSIL
                     ASIAGCFFVKAREGGKIMNALYRGLMVAGGLSAIGFWFITDWLMSPVVATNPELSTWH
                     LWAAGLIGLALT"
     gene            complement(1170..1772)
                     /locus_tag="VHT22_06850"
     CDS             complement(1170..1772)
                     /locus_tag="VHT22_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX3137308.1"
                     /translation="MPRNVEYFFTPVSPFTYLGHDRFVAIAGRHGVSIDVKPVDLGPV
                     FAVSGGLPLKQRAPQRQAYRLVELGRWSKHLGVPLNLQPRHFPVPPALASKWILAALS
                     QQDAGGALKLTSALLRAVWAEERNIAESDTLAAIASEQGFDAASLAERAAAADIRERY
                     DALTQEAIDRQVFGAPTYIYRDEPFWGQDRLDFLDRALAK"
BASE COUNT          364 a          662 c          643 g          310 t
ORIGIN      
        1 cggtcagcgc aaggccgatc agacccgccg cccacagatg ccaggtcgac aactcgggat
       61 tggtcgctac gaccgggctc atcagccagt cggtgatgaa ccagaaaccg atcgccgaca
      121 ggccaccggc gaccatcagc ccacggtaga gcgcattcat gatcttgccg ccctcgcgcg
      181 ccttgacgaa gaaacatccg gcgatcgagg ccaggatcga gaagccgcca agcgccagcg
      241 gatacgtcac cgcggcctcg gcgtacgccg gcagcagcag cgcacccagc agcatcgtcg
      301 cgacgatcgt caccgcgtag gtttcgaaca ggtcggccgc catgccggcg cagtcgccga
      361 cgttgtcgcc gacgttgtcg gcgatcaccg cgggattgcg cggatcgtct tccggaatgc
      421 cggcctcgac cttgccgacc aggtcggcgc cgacatcggc acccttggtg aatatgccgc
      481 cgcccaggcg cgcgaatatc gatatcagcg aaccgccgaa cgcgagcccg accagcggct
      541 cgatgatgcc gtgcaggcct tccttggtcg cgttcggcgc gtagtgcgcc gattgcacca
      601 ggaaccagta gaagcccgcc accccgagca gacctaggcc gacgaccagg aggccggtga
      661 tcgcaccgcc gcgaaaggcg accgccagcg cctcgttcag gccggtacgc gccgcctccg
      721 cggtgcgcac gttggagcgc accgagacgt tcatgccgat gaatccggca agtcccgaca
      781 gcactgcgcc gacggcaaag ccgatggcgg tggtccagga gaggaacaca ccgatgatca
      841 cgagcagaat gatgccgacg accgcgatcg tcgcgtactg gcgattcagg taggcctgcg
      901 cgccctgctg gacggcggcg gcgatctcct gcatgcgcgc gttgccggca ggcttttgca
      961 ggatccaggt gatcgaccag ccgccataga ccagcgcggc gaccgcgcaa acgagcgcga
     1021 agacgagtgc ggttgacatg tatgtcccct aaatagtggc gagcgaatct ttgcgggtct
     1081 ttgttattgg cccggccgaa catgcgccgg gaggtggttc tcgtcggcgc tcgcgtcggg
     1141 gcaagcggac ggcgaaccgc caaaacatgc tattttgcca gcgcccggtc caaaaagtcg
     1201 agccgatcct gaccccagaa cggctcgtct cgatagatgt acgtcggcgc cccgaagacc
     1261 tggcggtcga tcgcttcctg tgtcagagcg tcataacgct cgcggatgtc cgcggcggcg
     1321 gcgcgttcgg ccagcgacgc ggcgtcgaat ccctgctcgg atgcgatcgc ggccagcgtg
     1381 tcgctttccg cgatattccg ctcctctgcc cacaccgcgc gcaacagcgc actggtcagc
     1441 ttgagcgcgc cgccggcgtc ctgttgcgac aacgcggcca gaatccattt cgaagcgagc
     1501 gccggaggaa ccggaaaatg gcgtggctgc agattgagcg gcacccccag atgcttcgac
     1561 cagcgtccga gctcgaccag ccgatacgct tggcgctgcg gcgcccgctg cttcaacggc
     1621 aggccgcccg acaccgcgaa caccggacct aggtccaccg gcttgacgtc gatcgagacg
     1681 ccgtgccggc cggcgatggc gacgaatcgg tcgtgtccca ggtaagtgaa cggcgagacc
     1741 ggcgtgaaga aatattcgac gttgcggggc acgggcttcc tcttcgacgc agttgggtct
     1801 gttcgcctgg ctgtcgtcag actgaaaacg atttcgccag ctttttcgcg accgcgacat
     1861 tcttcagccg cacgtattgc ggcaggccgt cgcggaacgg gggaaacgct tcgccgcgcg
     1921 tcagcggcga caggtaggcg cgcgccttgg cggtgatgcc gtagccgtcc ttcgcgatg
//
LOCUS       DATANS010000463         1543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_402255, whole genome shotgun sequence.
ACCESSION   DATANS010000463 DATANS010000000
VERSION     DATANS010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402255"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1543)
                     /locus_tag="VHT22_06855"
     CDS             complement(<1..>1543)
                     /locus_tag="VHT22_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEX3137309.1"
                     /translation="VRFATVERDRVIEALESALRIGRGRVALHVLDDAERVVRVWRFS
                     SDLHCADCDIHYREPTPSLFSFNSPLGACESCRGFGRIIGIDFGLIVPDEAKSLRGGA
                     VRPWQTESYRECQDDLVRVARKRGVPLDTPWRELGAEHRKWVLEGEGSWEDKLWYGVR
                     RFFAWLETKAYKMHIRVLLSKYRAYTPCTACGGARLKTDALLWRLGTQQDAAIVLTDA
                     ARFAPSGTRWNAQTMAKLAGVGIRELMLLPIERCAAFFERLVLPAPLDEASDLVLREI
                     RSRLKFLLDVGLGYLTLDRQSRTLSGGEVQRINLTTALGTSLVNTLFVLDEPSIGLHP
                     RDMGRVIDVMKRLQESGNSLLVVEHDPQVMQAADRILDVGPGPGEKGGQIVFFDRPAQ
                     LPGAQRSLTADYLTGRKQVNGHVPIRNWEGATSLYVRGARQHNLKGIDVVLPLSRLVC
                     ITGVSGSGKSTLVQDVLYPALLKRFGKPTEVPGAHDRIEGADALGAVVMVDQAQIGRT
                     TRSNPA"
BASE COUNT          274 a          531 c          489 g          249 t
ORIGIN      
        1 cgctggattc gagcgcgtcg tgcgtccgat ctgcgcctga tcgaccatca cgactgcgcc
       61 gagcgcgtcg gccccttcga tgcgatcgtg cgcgccgggc acttcggtcg gcttgccgaa
      121 acgcttgagc aatgcaggat agagaacgtc ctgcaccagc gtcgacttgc cggagcccga
      181 tacgccggtg atgcagacga gccggctcag cggcagcacg acgtcgatac cttttagatt
      241 gtgttggcgc gcgccgcgga cgtacagcga ggtcgcgccc tcccagttcc ggatgggaac
      301 gtgtccgttt acttgtttcc tgcccgtcag gtaatccgca gtcagcgagc gctgcgcgcc
      361 cggcaactgc gcgggccgat cgaagaacac gatctgcccg cccttctctc ccggccccgg
      421 cccgacgtcg agaatgcgat ccgccgcctg catcacctgc gggtcgtgct cgaccacgag
      481 cagcgaattg cccgactcct gcaagcgctt catgacgtcg atcacgcgcc ccatgtcgcg
      541 cggatgcagg ccgatcgacg gctcgtcgag cacgaacagg gtgttgacca gcgacgtgcc
      601 gagcgccgtc gtcagattga tccgctgcac ttcgccgccg gacagcgtgc gcgactgccg
      661 atcgagcgtg agatagccga gaccgacgtc gagcaggaac ttcaggcggc tgcgaatctc
      721 gcgcagcacg agatcgctcg cttcatcgag cggcgcgggc agcacgagcc gttcgaagaa
      781 tgcggcacag cgttcgatcg gcaacagcat cagctcgcga atgccgacgc cagccagctt
      841 cgccatcgtc tgcgcattcc agcgcgtgcc gctgggcgcg aagcgcgctg cgtcggtgag
      901 aacgatcgct gcatcctgtt gcgttcccag tcgccacagc agtgcgtcgg ttttcaaacg
      961 cgctccgccg caggcggtac acggggtata cgcgcgatac ttcgacagca gcacgcggat
     1021 gtgcatcttg taagccttgg tctcgagcca ggcgaagaag cgccgcacgc cgtaccagag
     1081 cttgtcttcc cagctgccct cgccttccag cacccacttg cgatgctcgg caccgagctc
     1141 gcgccacggc gtatcgagcg gcacgccgcg cttgcgcgcg acgcgcacca gatcgtcctg
     1201 acactcgcgg tacgactcgg tttgccacgg ccgcacggcg ccgccgcgca gcgacttcgc
     1261 ttcgtcgggg acgatgagtc cgaagtcgat accgatgatg cggccgaagc cgcggcaact
     1321 ctcgcaggcg ccgagcggcg agttgaagga gaacaggctg ggcgtcggct cgcggtaatg
     1381 aatgtcgcaa tcggcgcagt gcaggtccga agagaatcgc cagacgcgca ccacccgttc
     1441 cgcatcgtcg agcacgtgga gcgcaacgcg cccacggccg atgcgcaatg ccgactccaa
     1501 cgcctcgatc acacgatcgc gctcgaccgt ggcgaaacgc acg
//
LOCUS       DATANS010000464         2059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_654016, whole genome shotgun sequence.
ACCESSION   DATANS010000464 DATANS010000000
VERSION     DATANS010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654016"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VHT22_06860"
     CDS             <1..238
                     /locus_tag="VHT22_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX3137310.1"
                     /translation="MSDLCAAALAGKLKEAREVNARLMPLHAKLFVEANPIPVKWALA
                     EMRMIAPDLRLPLTPLAAQYHDTVRAALKRAACL"
     gene            250..1398
                     /gene="bamC"
                     /locus_tag="VHT22_06865"
     CDS             250..1398
                     /gene="bamC"
                     /locus_tag="VHT22_06865"
                     /inference="COORDINATES: protein motif:HMM:NF018509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEX3137311.1"
                     /translation="MHLFSPLRPWPRPALAAAIAAAVAAVLLSGCETMTFNMGKKIDY
                     KSAGSAPALEVPPDLTTPTYDDRYQSTTASGAVAARAAPRPSEVLPTNPDARVARAGS
                     ERWLVVKASPEAAWATLKDFWMKNGFVLAVEQPALGIMETDWAEGRADKPEGWLQQFA
                     NKYVSFAVDTYKRDKFRTRIERGSEPGTVEIYVSHRGSEQKPMKTGSGGSPEDWNWTP
                     TPPNPQLEAEFLERLMVALGTPAPAAAQAVAADSKTPDRARIDTGADGVRLVVDDTFD
                     RAWRRVGLALDRTGFTVVDRDRSKGLYFVRYANPELEKKKSEGWLGDTLTKMQFWKTT
                     PPDKPEQYRVVVTQADPLSVVTVQDPNGVVDRTANSEKILALLKDQLK"
     gene            1416..>2059
                     /locus_tag="VHT22_06870"
     CDS             1416..>2059
                     /locus_tag="VHT22_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3137312.1"
                     /translation="MRFACLGSGSEGNGLLVECGDTRVLIDCGFGVRDAVARLARLGV
                     EAESVTAIIVTHEHSDHVGGVAAFAARYGTAVWLTFGTLATVAERFAELPRVYGFDSH
                     DRFAIDDIEVRPFPVPHDAREPVQFVCSDGRWRIGVLTDLGVSTAHVEASLSGCDALV
                     LECNHDVDMLANGDYPYPLKQRIAGKQGHLDNAAAAALLGRLDNSRLKHLFAAH"
BASE COUNT          360 a          675 c          693 g          331 t
ORIGIN      
        1 gatgtccgat ctgtgcgcgg cggcactggc cggaaagctc aaggaggcgc gcgaggtcaa
       61 cgcgcggctg atgccactgc acgccaagct gttcgtcgag gccaacccga tcccggtcaa
      121 atgggcgctc gccgaaatgc gcatgatcgc gccggatttg cggctcccgt tgacgccgct
      181 cgccgctcaa tatcacgaca ccgtccgcgc ggcgctcaag cgcgcggcct gcctgtagga
      241 gaagttcgta tgcatctctt ttccccgttg cggccctggc cgcgccccgc tctcgccgcc
      301 gccattgccg ccgctgtcgc cgccgtgctg ttgtccggct gcgaaacgat gacgttcaat
      361 atgggcaaga agatcgatta caagtccgca gggtcggcgc cggcgctcga ggtgccgccc
      421 gacctgacga cgccgaccta cgacgatcgc tatcagtcga cgaccgcctc gggcgcagtc
      481 gcggcgcgcg cggcgccgcg cccgagtgag gtgttgccga ccaatccgga cgcccgcgtc
      541 gcgcgcgccg gcagcgagcg ctggctggtc gtcaaggcga gcccggaggc cgcctgggcg
      601 acgttgaagg acttctggat gaagaacggc ttcgttctcg ccgtcgagca gccggcgctg
      661 ggaatcatgg aaaccgactg ggcggagggc cgcgccgaca agcccgaggg ctggctgcaa
      721 cagttcgcga acaagtacgt cagcttcgcg gtcgatacct acaagcgcga caaattccgg
      781 acgcggatcg agcgaggcag cgagccaggc acggtcgaga tctacgtcag ccaccgcggc
      841 tccgagcaga agccgatgaa aacggggtcg ggcggatcgc cggaagactg gaactggacg
      901 ccaacgcctc ccaacccgca gctcgaagcc gaattcctcg aaaggctgat ggttgcgctc
      961 ggcacgccgg cgccggcggc ggcgcaggcg gttgcggccg acagcaagac tcccgaccgg
     1021 gcgcgcatcg acacgggcgc cgacggcgtt cggctggtcg tcgacgatac gttcgatcgc
     1081 gcctggcgcc gcgtcggcct ggcgctcgat cgcaccgggt tcaccgtcgt cgaccgcgat
     1141 cggtcgaagg gcctgtattt cgtccgctac gccaaccccg agctcgagaa gaaaaaatcc
     1201 gaagggtggc tgggcgatac cctgaccaag atgcagttct ggaaaacgac gcctccagac
     1261 aagcctgaac agtatcgcgt cgtcgtgacc caggccgatc cgctgagcgt agtcaccgta
     1321 caggatccga acggcgtcgt cgaccgcacc gccaacagcg agaaaattct tgcgctgctc
     1381 aaagaccagt tgaagtaaag cgggaacagg ccccagtgcg tttcgcctgt ctcggcagcg
     1441 gcagcgaagg caacggcctg ctggtcgaat gcggcgacac gcgcgtgttg atcgactgcg
     1501 gcttcggcgt tcgcgatgcc gtcgcgcgtc ttgcccggct gggcgtggaa gcggagagcg
     1561 tcaccgcgat catcgtcacg catgagcaca gcgatcatgt cggcggcgtg gcggcgttcg
     1621 ctgcgcgtta cggcacggcg gtgtggctga ccttcggcac tctggccacc gtcgccgagc
     1681 gcttcgcgga gctcccgcgc gtgtacggat tcgacagcca tgatcgtttt gcgatcgacg
     1741 acatcgaagt gcggccgttt ccggtgccgc acgatgctcg cgagccggtc caattcgtct
     1801 gttctgatgg ccggtggcgg atcggcgttc tgaccgacct gggggtctcg accgcgcacg
     1861 tggaggcgag cctgtcaggc tgcgacgcgc tggtgctcga atgcaatcac gacgtcgaca
     1921 tgctggccaa tggcgattac ccctatccgc tgaagcagcg catcgccggc aagcagggac
     1981 atctggacaa tgcggccgcg gcggcgctgc tgggacgtct ggacaactcg cggctcaagc
     2041 atctgttcgc cgcgcattt
//
LOCUS       DATANS010000465         2238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_738724, whole genome shotgun sequence.
ACCESSION   DATANS010000465 DATANS010000000
VERSION     DATANS010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738724"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="VHT22_06875"
     CDS             complement(<1..1311)
                     /locus_tag="VHT22_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3137313.1"
                     /translation="MSAYHAPLTEIRFVLRELAGLDQVGKLPGFEDATHDTVDAILEE
                     AARFATEVLDPLNAAGDREGAQWQEGGIVRTPAGFKEAYRHFAENGWNGLTKNPEHGG
                     QGLPQLVATAVEEMWHGANMAFALCPLLTQGAIEALELCGSDALKAMFLDKMVAGVWT
                     GTMNLTESQAGSDLAAVRTRALPQPDGSFRVYGQKIFITYGEQDYTDNIVHLVLARTP
                     TAPEGVKGISLFVVPKFLVNADGSLGERNDVHCVSIEHKLGIHASPTAVLAYGDHGGA
                     VGYLVGEENRGLEYMFIMMNLARFSVGIEGIGIAERAYQRARAYARDRVQGRAPGLDR
                     ATPTATILQHPDVRRMLLTMKAQTEAMRAVAYVTGAAIDNARRHPDAEARKQHQAFVD
                     LMIPIVKGWSTELAQEVTYLGVQVHGGMGFIEETGAAQDYRDARI"
     gene            complement(1311..2237)
                     /locus_tag="VHT22_06880"
     CDS             complement(1311..2237)
                     /locus_tag="VHT22_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX3137314.1"
                     /translation="MILVVAEHDHAALKSATLNTVAAAQKIGGDVHLLVAGHNAASAA
                     AAAAQVGGIAKVLHADAAQLAAQTAENLAALVVGLAAPYAHILLPATGFGKNAAPRIA
                     ALLDVAQISDIVAVEGPDTFVRPIYAGNAFATVQSKDAKKVVTVRTTGFDPVASSGGT
                     APVESVAAPADTGLTQVLGQELTRSSRPELTSARVVISGGRGMQNGENFKMLEALADK
                     LHAAIGASRAAVDAGYVPNDYQVGQTGKIVAPDLYIAIGISGAIQHLAGMKDSKVIVA
                     INKDADAPIFQIADYGLVGDLFEIVPALTAEL"
BASE COUNT          341 a          760 c          727 g          410 t
ORIGIN      
        1 gattcgcgcg tcgcgataat cctgcgctgc gccggtttcc tcgatgaagc ccatgccgcc
       61 gtgtacctgg acgcccaggt aggtgacttc ctgcgcaagc tcggtgctcc atcccttgac
      121 gatcggtatc atcagatcga cgaatgcctg gtgctgcttg cgggcttcgg cgtccggatg
      181 ccggcgcgcg ttatcgattg cagcgccggt gacgtaggcg acggcgcgca tcgcctccgt
      241 ttgcgccttc atcgtcagca acatgcggcg aacgtccgga tgctgcagaa tcgtcgccgt
      301 cggcgtcgcc cggtcgaggc ccggcgccct gccctgcacg cgatcgcgcg cataggcgcg
      361 cgcgcgctga taggcgcgct cggcgatgcc gatcccttcg atgccgaccg aaaagcgcgc
      421 gagattcatc atgatgaaca tgtattcgag gccgcgattc tcctcgccga ccagatagcc
      481 gacggcgccg ccatggtcgc cgtaagcgag caccgccgtc gggctcgcgt ggatgcccag
      541 cttgtgctcg atcgaaacgc aatgtacgtc gttgcgctcg ccaaggctgc cgtcggcgtt
      601 gaccaggaat ttcggcacga caaaaagcga tatgcccttg acaccctcgg gcgccgtcgg
      661 tgtccgcgcc agcaccagat gcacgatatt gtcggtgtag tcctgctcgc cgtaggtgat
      721 gaagatcttc tggccgtaga cgcggaagct gccgtcgggc tgtggcagcg cgcgcgtgcg
      781 cacggcggca agatcggagc ctgcctgcga ctcggtcagg ttcatcgtcc ccgtccagac
      841 gcccgcgacc atcttgtcga ggaacatcgc cttcagcgcg tccgagccgc aaagctccag
      901 cgcctcgatc gcgccctggg tcagcagcgg gcacaacgcg aacgccatat tggcgccgtg
      961 ccacatttcc tcgaccgccg tggcgaccag ttgcggcaag ccctggccgc cgtgctcggg
     1021 gttcttggtc aggccgttcc agccgttttc agcaaagtgc cggtaggcct ccttgaatcc
     1081 cgctggcgtc ctgacgattc cgccttcctg ccactgcgcg ccttcgcgat cgccggcggc
     1141 gttcaaagga tcgagcacct cggtcgcaaa cctggccgct tcctcgagga tcgcgtcgac
     1201 ggtgtcgtgt gtcgcgtcct cgaacccggg cagtttgccg acctgatcga gcccggcgag
     1261 ctcgcgaagg acgaagcgga tctcggtgag cggcgcgtga taggcgctca tcagagctcg
     1321 gcggtcagcg ccggaacgat ttcgaacaaa tcgccgacca ggccgtagtc ggcgatctgg
     1381 aatatcggcg catcggcgtc cttgttgatc gcgacgatca ccttgctgtc cttcatgccg
     1441 gccagatgct ggatcgcgcc ggagatgccg atcgcgatat acagatccgg ggcgacgatc
     1501 ttgcccgtct ggccgacctg gtagtcgttg ggcacgtatc cggcatcgac cgccgcgcgc
     1561 gaggcgccta tcgcagcgtg caacttgtcg gccagcgcct cgagcatttt gaaattctcg
     1621 ccgttctgca tccctcggcc gccggaaatg acgactcgcg cgctggtaag ctcgggccgg
     1681 ctggatcggg tgagctcctg acccagcacc tgggtcaggc ccgtatccgc cggcgcggcc
     1741 acgctttcga ccggcgcggt gccgccgctg gacgcgaccg gatcgaatcc cgtcgtccgc
     1801 acggtgacga cctttttcgc gtccttcgac tgaacggtgg cgaaggcgtt gccggcatag
     1861 atcggacgca cgaaggtgtc cggcccttcg accgcgacga tgtcggagat ctgcgcgacg
     1921 tcgagcagcg cggcgatccg cggagctgca ttcttgccga agcccgtcgc cggcagcagg
     1981 atgtgcgcat acggcgccgc cagcccgacc accagcgcgg ccaggttctc cgccgtctgc
     2041 gcggcgagct gcgccgcatc ggcgtgcagc accttggcga ttccgccgac ctgcgccgcg
     2101 gcggctgccg cgctcgcagc gttgtgcccg gctaccagca ggtgcacgtc gccgccgatc
     2161 ttttgcgcag cggcgaccgt attgagtgtc gcgctcttca gcgccgcgtg gtcgtgctcg
     2221 gcgacgacga ggatcatg
//
LOCUS       DATANS010000466         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_193263, whole genome shotgun sequence.
ACCESSION   DATANS010000466 DATANS010000000
VERSION     DATANS010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193263"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /gene="trpD"
                     /locus_tag="VHT22_06885"
     CDS             <1..798
                     /gene="trpD"
                     /locus_tag="VHT22_06885"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX3137315.1"
                     /translation="MVGTGGDGSGSFNLSTGAALLVAAMGIRVAKHGTSSVSSRSGSA
                     DCVRALGLNLPLDEKAAGECLAATGFTFFFAPHYHPAMKAVMPIRQALGVRTIFNILG
                     PLSNPAEPPFHLIGAYHVDIAALMAETLAGMPIERAFVVHGEPGWDEATPIGPFTLFD
                     VRPGEVKRTRRSAEEFALPACTADELKGGDAAYNAEHLRAVLEGRDRGAHRNAIVLQA
                     ALVLELMNKASSATAAASMAEDAIESGAGRRILDKIATFGRREALAG"
     gene            803..1681
                     /locus_tag="VHT22_06890"
     CDS             803..1681
                     /locus_tag="VHT22_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HEX3137316.1"
                     /translation="MSGLLNEMAQSSLARLEQARARESEQALWTRVCSAPTAPRLKLS
                     PEGFDIIAECKLASPSAGDLSQHTSDIDRRVQAYAQGGACAVSVLTEPTRFSGSLEHL
                     AQTARTLAPLGVPVMRKDFLVDPYQVMEARAAGAGGVLVIVRMLDRSRIKALLDVAAM
                     LKMFVLIETFDAADLAVAAETLKERKAHDEQMLVGVNCRDLDTLSVELSRLGELAGQL
                     PPDFASVAESGVGSLEDVKTVVDVGYDVALVGTTLMNSNDPGKLLGDMLAAGRERAMA
                     VRTRKMRITGASMDDE"
     gene            1683..>2180
                     /locus_tag="VHT22_06895"
     CDS             1683..>2180
                     /locus_tag="VHT22_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-(5'-phosphoribosyl)anthranilate isomerase"
                     /protein_id="HEX3137317.1"
                     /translation="MALWVKICGLTTRDAVEAAVAAGADAVGFVFAPSKRQVTAALAT
                     QLAQGVPRRILRVAVMLHPTQSELDDVWSEFRPDVLQTDVGDLQTLRVPMGLGVMPVV
                     RGGGGVGLKPDLQPKHSRLLFEGPVSGVGSTSDWSAAAQLARTTQLVLAGGLNATNIA
                     DAIAAV"
BASE COUNT          366 a          661 c          766 g          387 t
ORIGIN      
        1 atggtcggca ccggcggcga tggctcgggc agcttcaatc tttcgacggg agcggcgctg
       61 ctggttgctg cgatgggcat tcgcgtcgcc aagcacggca ccagttcggt ctcgagccgc
      121 agcggcagcg ccgattgcgt ccgcgcgctc ggcttgaatc tgccgctcga cgagaaggcg
      181 gccggcgagt gtctcgcggc caccggcttt acgttcttct tcgcgccgca ttaccacccg
      241 gcgatgaagg cagtgatgcc gatccggcaa gcgctcggtg tgcgcacgat cttcaacata
      301 ctgggtccgc tgtcgaatcc tgcggagccg ccgtttcact tgatcggcgc gtaccacgtc
      361 gacatcgcag ccctgatggc cgagacgctc gcgggcatgc cgatcgagcg cgccttcgtc
      421 gtgcacggcg agccaggctg ggacgaagcg acgccgatcg gccccttcac gttgttcgac
      481 gtgcggccgg gcgaggtcaa acgcacgcgt cgtagtgcag aggaatttgc gctgcctgcc
      541 tgcactgccg atgagctcaa gggcggcgac gctgcttaca atgccgagca tctgcgtgca
      601 gtactcgaag gtcgcgaccg cggcgctcat cgcaacgcca tcgtgttgca ggcggcgctc
      661 gtgctggagc tcatgaacaa ggcgtcgtca gcgacggctg ccgccagcat ggccgaagac
      721 gcgatcgagt cgggtgcggg acgtcggata ctggacaaga tcgccacgtt tggacgcaga
      781 gaagcactag caggatagct ctatgtcagg acttctcaat gagatggcgc agtcgagcct
      841 tgcgaggctc gaacaggcgc gcgcacggga atccgagcag gcgttgtgga cgcgcgtgtg
      901 ctcggcccca acggcaccgc gcttgaagct ttcgccggaa ggcttcgaca tcattgccga
      961 gtgcaagctc gcgtcccctt cggctggcga tctgtcgcag cacacgtccg acatcgacag
     1021 gcgcgtgcag gcgtacgcac aaggcggcgc ctgcgccgtt tccgtgctga ccgagccgac
     1081 gcgattcagc ggctcgctcg agcatctcgc gcagacggcg cggacgctcg cgccgcttgg
     1141 cgtgccggtg atgcgcaagg attttctcgt cgatccgtat caagtcatgg aagcgcgggc
     1201 tgcgggtgca ggcggcgtgc tcgtgatcgt gcgcatgctg gatcgcagtc gcatcaaggc
     1261 gctgctcgac gtcgccgcga tgctgaagat gttcgtcctc atcgaaacgt tcgacgctgc
     1321 cgacctggcg gtcgcggcgg aaacgctcaa ggagcgcaag gcgcacgacg agcagatgct
     1381 ggtcggcgtc aattgccgag atctcgacac actcagcgtc gagttgtcgc ggctcggtga
     1441 gctggcgggt cagttgccgc ctgacttcgc ttcggtagcc gaaagcggcg tcggctcgct
     1501 ggaagacgtc aagactgttg tcgatgtcgg ttacgacgtc gcgctcgtcg gcacgacgct
     1561 catgaattcg aacgatcccg gcaagctgct cggcgacatg ctcgcagccg gacgcgagcg
     1621 cgcgatggca gtgcgcactc gcaagatgcg gatcaccggc gcttcgatgg acgacgagta
     1681 gcgtggcgct ttgggtcaag atctgcggcc tcaccacacg cgatgcagtc gaagcagcgg
     1741 tcgctgcggg cgccgatgcg gtcggcttcg tgttcgcgcc gtccaagcga caggtgacgg
     1801 cagcgctggc gacgcagctc gcccaaggag tcccgcggag aattctgcgg gtcgcggtga
     1861 tgctgcatcc gacgcagagc gagctggatg acgtgtggtc ggagtttcgg ccggatgtgc
     1921 tgcagacgga cgtgggggat ttgcagacgt tgcgggtgcc gatggggttg ggagtgatgc
     1981 cggtggtgag agggggaggg ggagtcgggc tgaagcccga cctacagccc aaacattccc
     2041 gtctgctctt tgaaggaccc gttagtggcg tcgggagcac gtcggattgg agcgcggcag
     2101 cgcagctcgc gcgcacgacg cagctcgtgc tcgctggcgg actcaacgcg accaacattg
     2161 ctgacgcgat cgctgcggtt
//
LOCUS       DATANS010000467         2535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_527093, whole genome shotgun sequence.
ACCESSION   DATANS010000467 DATANS010000000
VERSION     DATANS010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527093"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            358..558
                     /locus_tag="VHT22_06900"
     CDS             358..558
                     /locus_tag="VHT22_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HEX3137318.1"
                     /translation="MARYGKKAQSKVEKTMRERKRGTLRSGRSGKKVTSRKQAIAIGL
                     SEARRAGAKVPKRRSSLSSRGS"
     gene            complement(646..1545)
                     /locus_tag="VHT22_06905"
     CDS             complement(646..1545)
                     /locus_tag="VHT22_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006749029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEX3137319.1"
                     /translation="MDAWPSLSYAAWKDTYATLLLWTQIVGKIRLVQTPWVNHSWHVV
                     YYVTARGLSTSPIRHGERVFQIDFDFIDHQLVIQTGEGERRTLRLEPRTVADFYQALM
                     ALLAELDLRVAINTTPNEQVEAIPFDKDQKNRSYDADAVRRFFAALLQADRLLKQFRA
                     GFNGKCSPVHFFWGGFDLAVTRFSGRPAPEHPGGVPHLPDRVAREAYSHEVSSCGFWP
                     GNEALPEAAFYAYAYPEPDGFRNADVRRPAYYHPDLREFILSYDEVRKAPSPDAVVLD
                     FLQSTYEAAATLGRWDEVGRQPT"
     gene            complement(1555..>2535)
                     /locus_tag="VHT22_06910"
     CDS             complement(1555..>2535)
                     /locus_tag="VHT22_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX3137320.1"
                     /translation="LQAQGIAHARCAGMIETCEESGSYDLPAYLEALAPRMGNVDFVV
                     GLDSGCGDYERLWATTSLRGLAGGTLSVEVLTEGVHSGDASGIVPSSFRIARKLLDRI
                     EDASTGRVLPPEFHAPLPAERAEQARRAADVLGDIVIGKYPFAGATKPMTSDRAEALL
                     NRTWRPALSITGADGLPPIADGGNVLRPQTALKLSLRLPPTVDGAAAERAMQRLLEAD
                     PPYGATVRFTGESGATGWNAPPTDAWLARAIDEASSHFYGKPSAAMGEGGTIPFMAML
                     GKHFPDAQFLITGVLGPHSNAHGPNEFLHVPYASKLTACVAYVLAAHAGR"
BASE COUNT          452 a          814 c          860 g          409 t
ORIGIN      
        1 gccgccggtc ggcatcagga cgagcgcgtc gccgccgccg acgacgtggt cgacgacttc
       61 gccctggccg gcgcggaagc tcgagttgcc gaaggtctgc cgcagcagct cgcgcgccgc
      121 gtcggcaggt gacatgttgc ccatgaacgc aattgtagtc gatggttgcg acgccaaatc
      181 caacccgttc ggacgagtcc tacttgcgtc tgcgtcagct cctaccgctg cacggcgtgt
      241 caaccgacgc gatccccttc gggccgcgat tcgcggaaca atcgccgatg cgcgcggttc
      301 caaggtatac gcgttgcaga cagattgctg ctgcgcagtt gccaaaggag atccatcatg
      361 gccagatacg gcaagaaggc tcaaagcaag gtcgaaaaga cgatgcgcga gcgcaaacgc
      421 ggaaccttgc gcagcggacg gtcgggcaag aaggtgacca gccgcaagca ggcgatcgcg
      481 atcggcctgt cggaagcgcg ccgcgccggc gccaaggtgc cgaagcggag atcgtcttta
      541 tcgtcgaggg gatcgtagcg gtcgcgagac accggccggc gagttcgctg gaaccgctgg
      601 ccggcaatac aacaagtcgg ccgttagctc ggcgcgaggc gctactcagg tcggttgacg
      661 tccgacttca tcccaccgtc cgagcgtagc ggcggcttcg taggtgctct gcaggaaatc
      721 aagcaccacc gcatccggtg agggcgcctt gcgcacctcg tcgtaggaca ggatgaattc
      781 acgcaagtcg ggatggtaat aggcagggcg ccgcacgtcg gcattcctga atccgtcggg
      841 ctcgggatac gcatacgcgt agaacgccgc ttcaggcagc gcttcgttgc ccggccagaa
      901 tccgcagctg ctgacttcgt gcgagtacgc ctcgcgcgcg acacggtccg gcaaatgcgg
      961 caccccgccc gggtgctcgg gggcgggacg gccggaaaat cgcgtcactg cgaggtcgaa
     1021 gccgccccag aaaaagtgca cagggctgca tttgccgttg aagccagccc ggaactgctt
     1081 gagcaggcga tcggcctgca gcagcgccgc aaaaaatctg cgaacggcgt ccgcatcgta
     1141 cgagcgattc ttctggtctt tatcgaacgg gatcgcttcg acctgctcgt tgggcgtcgt
     1201 attgatggcg actctcaggt ccagctccgc cagcagcgcc atcagcgcct ggtagaagtc
     1261 cgccaccgtg cgcggctcga gccgcagggt tcgacgctcg ccttcgccgg tctggatgac
     1321 cagctggtgg tcgatgaagt cgaaatcgat ctggaagacg cgctcgccgt gccggatggg
     1381 cgaagtcgaa aggccgcgcg cggtgacgta atagacgacg tgccacgagt gattgaccca
     1441 cggcgtctgc accagtcgga tcttgccgac gatttgcgtc cacagcagca acgtcgcgta
     1501 ggtgtctttc cacgcagcgt aggaaaggct gggccaggcg tccatcgcgg tcgactagcg
     1561 cccggcgtgc gccgccagca cgtacgcgac gcaggcggtc agcttgcttg cgtagggcac
     1621 atgcaggaac tcgttcgggc cgtgggcgtt ggagtgcggg cccagcacgc cggtgatcaa
     1681 aaactgcgcg tcgggaaagt gcttgccgag catcgccatg aaggggatcg ttccgccttc
     1741 gcccatcgcc gccgacggct tgccgtaaaa gtgcgacgag gcttcgtcga tcgcgcgcgc
     1801 aagccaggca tccgtcggcg gtgcgttcca tccggtcgcg cccgattcgc cggtaaagcg
     1861 caccgtggcg ccatacggcg gatcggcttc gagcagtcgt tgcatcgcgc gctcggcggc
     1921 cgcgccatcg acggtcggag gcagccgcag cgagagtttc agcgcggttt gcggccgcag
     1981 cacgttgccg ccgtcggcga tcggcggcaa gccatcggcg ccggtgatcg acagggcagg
     2041 gcgccaggtc cggttgagca acgcttcggc acggtcgctg gtcatcggct tcgtcgcgcc
     2101 ggcgaacgga tatttgccga tcacgatatc gccgaggaca tcggctgcgc gccttgcctg
     2161 ctcggcgcgc tcggcgggaa gaggcgcgtg gaattcgggc ggcagcacgc ggccggtcga
     2221 cgcatcctcg atccggtcga gaagcttgcg cgcaatgcga aacgacgaag gcacgatgcc
     2281 gctggcgtca cccgaatgca caccctcggt cagcacctcg acgctcaagg tgccgccggc
     2341 caggccgcgc agcgacgtcg tcgcccagag gcgttcgtag tcgccgcagc ccgaatcgag
     2401 gccgacgacg aaatcgacgt tgcccatgcg cggcgccagc gcctcgagat aggcgggcag
     2461 gtcgtagctg ccgctttcct cgcaggtttc gatcatgccg gcgcatcgcg catgcgcgat
     2521 cccctgcgcc tgcag
//
LOCUS       DATANS010000468         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_149783, whole genome shotgun sequence.
ACCESSION   DATANS010000468 DATANS010000000
VERSION     DATANS010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149783"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="VHT22_06915"
     CDS             complement(<1..1253)
                     /locus_tag="VHT22_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3137321.1"
                     /translation="MSTFAPHRRGAAWTLAVLLSSAIAAAVAPAHAADSAKVLHVAIP
                     VAETGFDPQAAGDAYSNYINRVIFDALYKYDYLARPFKLVPNTAAALPDISADRLTWT
                     IKVRPGIYFADDPVFKGRRRELTAADYVYGLKRTLDPAMRSNSLNVFEGRFVGADAVV
                     AKARQSGKFDYDAPIEGLRVLDRYTLRLQLNFPDAELLSNLSITGSAAVAREVIEAYG
                     ETNGWTMANPVGTGPYRLAEWRRGQRIVLEANSGFREERYPASSDGADGAPAKGLAGK
                     RMPMIGRIEISIIEEANPRLLSFEQDLQDYLLVPPELASNVLDPGNKLKQRLAQKGIR
                     LERDAQPVITGLAFNMEDPVVGGYDKSRIALRRAIAMAYDAEEDARVIRHGQARRATQ
                     FIPPNVSGYDAKIDKPARRDVAGARA"
     gene            complement(1255..>1520)
                     /locus_tag="VHT22_06920"
     CDS             complement(1255..>1520)
                     /locus_tag="VHT22_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013145288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEX3137322.1"
                     /translation="GGEEEIESFCEVNYGVTFPMFAKIDVNGKNAAPLYEYLKDAKPG
                     LLGSEAIKWNFTKFLVDRKGKVVERYAPKVEPEAIADDIEKLL"
BASE COUNT          259 a          487 c          486 g          288 t
ORIGIN      
        1 gcgcgagcac ccgcgacgtc gcgtcgcgcc ggcttgtcga ttttcgcgtc gtagccgctg
       61 acgttggggg gaatgaactg cgtcgcacgg cgggcctggc cgtgcctgat cacccgtgcg
      121 tcctcctccg cgtcataagc catcgcgatc gcgcggcgca gtgcgatcct gctcttgtca
      181 tagccgccga cgaccgggtc ttccatgtta aaggcgagac cggtgatcac cggctgcgca
      241 tcgcgctcga ggcggatgcc tttttgagcg agcctctgtt tgagcttgtt gccggggtcg
      301 aggacattgc tcgcaagctc gggcggaacg agcaggtaat cctgaagatc ctgctcgaac
      361 gaaagcaggc gcggattggc ttcctcgatg atgctgatct cgattcgccc gatcatcggc
      421 atccgcttgc ccgcgagccc tttcgctggc gcgccgtcgg cgccgtcgct gctcgccggg
      481 tagcgctctt cgcgaaagcc ggaattggct tcgagcacga tccgttggcc gcgacgccac
      541 tcggccagcc ggtacggacc ggtgccgacc ggattcgcca tcgtccatcc gttggtctca
      601 ccgtaggctt cgatgacttc gcgcgctacc gcggcgctgc cggtgatgga cagattcgac
      661 agcagctcgg cgtcgggaaa attgagctgc aggcgcaacg tatagcggtc gagaaccctc
      721 aagccttcga tcggagcgtc gtagtcgaac ttaccgctct gcctcgcctt ggcgacgacc
      781 gcgtcggcgc cgacgaagcg cccttcgaaa acgttcaacg agttcgagcg catcgcggga
      841 tcgagcgtgc gcttgagccc gtagacatag tccgcggcgg tgagctcgcg gcggcggccc
      901 ttgaacaccg gatcatcggc aaagtaaatg cccggcctga ccttgatcgt ccacgtcaag
      961 cgatcggctg agatatcggg caaggccgcc gcggtattcg gaaccagctt gaacggccgc
     1021 gccaggtaat cgtacttgta cagcgcatcg aaaatcacgc ggttgatgta gttcgaatag
     1081 gcgtcgcccg cggcctgcgg gtcgaagccg gtttcggcga ccgggatcgc cacgtgcaga
     1141 accttggcgg aatcggcggc gtgcgccggc gcgaccgccg ctgcgatcgc cgaggaaagc
     1201 aacaccgcga gggtccacgc ggcgccgcgc cggtgcggcg cgaacgtgga cacgctaaag
     1261 cagcttttcg atgtcgtcgg cgatcgcctc tggctcgacc ttcggggcat agcgctcgac
     1321 gaccttaccc ttgcggtcga ccaggaactt ggtgaagttc cacttgatcg cctccgagcc
     1381 cagcaagccg ggcttggcgt ccttcaagta ctcgtacaag ggcgcggcgt tcttgccatt
     1441 gacgtcgatc ttggcgaaca tcggaaaggt cacgccatag ttgacttcgc agaacgactc
     1501 gatctcttcc tcacctccgg
//
LOCUS       DATANS010000469         1665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_276679, whole genome shotgun sequence.
ACCESSION   DATANS010000469 DATANS010000000
VERSION     DATANS010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276679"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..612
                     /locus_tag="VHT22_06925"
     CDS             91..612
                     /locus_tag="VHT22_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX3137323.1"
                     /translation="MSRAFVKESDEDSAAAELPERPLSTAPNYVTPRGLEALRARLKE
                     LQNERERLAAAAEPMAQQRLLEIKRDIRYFTAQIDRATLVDMDGQPHDEVHFGARVTI
                     SDEQGKDHTFQIVGDDEADVAAGRISWASPLAKAMLGARVGDTVKWQRPAGPSEVEIV
                     AIDYSTASATKRT"
     gene            668..1591
                     /locus_tag="VHT22_06930"
     CDS             668..1591
                     /locus_tag="VHT22_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017508160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HEX3137324.1"
                     /translation="MTFLSLIGALLLEQWRPLRTGNRLYVAFARYVNRLGQNFNAGQY
                     RDGVISWVLAVLPVALITFIVYSVSRHVNGLLALAWNIVVLYLALGFRQFSHFYNDVM
                     LALRAGDLPRAREILTWWRGESARELTAGEVARVAIELGLIRSHRHVFGVMAWFIVLG
                     PVGAILYRLAALLADRWSRGRDAGADSGAFGHFARSAYAIIDWLPVRLTAFSFAMTGN
                     FTDAIECWREQAASWRARAQGILLAAAAGALGVKLGGVLHEEGGIAYRPQLGDGDDAD
                     VDYMQAAVGLIWRALVLWLFLILLATLASWF"
BASE COUNT          276 a          526 c          565 g          298 t
ORIGIN      
        1 tgacatatag ttgaacaatc tgagttgagc cgtctgagtc gagcaatcta agtattgccg
       61 atactgccgg aaaacggaac gggaaaaacc atgagccgcg catttgtcaa agagtccgac
      121 gaggattcgg ctgccgccga gctgcccgag cgcccgctga gcaccgcgcc gaattacgtg
      181 acgccgcgcg gcctggaagc gttgcgcgcg cgcctgaagg aattgcaaaa cgagcgcgag
      241 cggctggccg ccgccgccga gcccatggcc cagcagcggc tgctcgaaat caagcgcgac
      301 atccgctatt tcaccgcgca gatcgaccgg gcgacgctgg tcgatatgga cggccagccg
      361 cacgacgaag tgcatttcgg cgcccgcgtc acgatcagcg acgagcaggg caaggaccat
      421 acgtttcaaa tcgtcggcga tgacgaggcc gacgttgctg ccggccgcat cagctgggcc
      481 tcgccgctcg caaaggcgat gctgggcgcg cgggtcggcg acaccgtgaa gtggcagcgc
      541 ccggcgggcc cgtccgaggt ggagatcgtc gcgatcgact attcaacagc atcggcgacc
      601 aagcgtacat gacgcgcttg aatgcgcggt tggccgctga cggggcggcc gcggcagtgc
      661 aggcgccatg acctttttat cgctgatcgg cgcgctgctg ctcgagcaat ggcggccgct
      721 gcgcacgggc aaccgcctgt acgtcgcctt cgcgcgctac gtcaaccgcc tcgggcaaaa
      781 cttcaacgcc ggccagtatc gcgacggcgt tatctcgtgg gtgctggcgg tcttgccggt
      841 ggcgctgatc acgttcatcg tctacagcgt atcgcgccac gtcaacgggc tgctcgcgct
      901 cgcctggaac atcgtcgtcc tgtatcttgc gctcggcttt cgtcagttca gccatttcta
      961 caacgacgtg atgctcgcgc tgcgtgccgg cgatctgccg cgcgcgagag agatcctgac
     1021 ctggtggcgc ggcgaatcgg cacgcgaact aaccgcgggg gaggtcgcgc gcgtcgcgat
     1081 cgagctgggc ctcatccgct cgcatcgtca cgtgttcggc gtgatggcct ggttcatcgt
     1141 gctggggccg gtcggcgcaa ttctctatcg gctggccgcg ttgctggcgg atcgctggag
     1201 tagagggcga gacgccggcg ccgactccgg cgcgttcggc catttcgcgc gcagcgcgta
     1261 cgcgatcatt gactggctgc cggtgcgcct gaccgcattc agttttgcca tgaccggtaa
     1321 ttttacggat gcgatcgaat gctggcgcga acaggcagcg tcctggcgcg cgcgcgcgca
     1381 gggaatacta ctcgccgccg cggcgggcgc gctcggcgtc aagctcggcg gcgtgctgca
     1441 tgaggagggc ggcatcgctt atcggccgca actcggcgac ggcgacgacg ccgacgtcga
     1501 ttacatgcag gccgcggtcg ggctaatctg gcgcgcgctc gtgctctggc tgttcctgat
     1561 cctgctggcg acgctcgcga gctggttcta aaggcgagcg atcttaagcc gcgagccggt
     1621 tctaagtgag cgttcctcgt ctccgagtag gcgagcgccg gctat
//
LOCUS       DATANS010000470         1746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_193502, whole genome shotgun sequence.
ACCESSION   DATANS010000470 DATANS010000000
VERSION     DATANS010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193502"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /locus_tag="VHT22_06935"
     CDS             complement(<1..1312)
                     /locus_tag="VHT22_06935"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3137325.1"
                     /translation="MRALRAWLLRLGASLWGRARTGDVADELNSHLGFHIDDNIRAGM
                     TPDEARRAALLELGGFTQTVESIRDRRGLPFLDTLWQDLVYAVRVLRRSRGFTATAVL
                     TFALGVGANSAIFSIVNATLLRPLPLAEPSRLVMIYATEARRSLQFDGVSYPAFIDWQ
                     EQNRSFESMAAFANQSLPLGTGDEVVAARGKVVTANLFDVVGVQPAIGRAFREWRLQS
                     PDVVVLSDGFWKRHFGGSPAAIGQTVRIDERPHTIVGVMPSRFTLDGDSEDFFQPLGV
                     DANRNRGFLRTVGRLRRGVTLEQARDDLSTITRRLERLYPDSHAGVGANLVPMADAVA
                     RRVRMGLLTIMSVVGVVLLIACANVASLMLARGATRQQELALRAALGAGRFRLVRQLL
                     TESAVIAAAGGILGLLVAHWTASALAAAVADQFRVPGIESVRTDG"
     gene            complement(1309..1653)
                     /locus_tag="VHT22_06940"
     CDS             complement(1309..1653)
                     /locus_tag="VHT22_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX3137326.1"
                     /translation="MADKADVWQGTLALMVLKTLETLGPLHGYGIARRIEQTSAHHLS
                     LNYGTLYPALLKLEQEGYVASEWGVSDNNRKAKFYRLTRAGRKQLEREAREWERTTAI
                     LARFLTPGEEAP"
BASE COUNT          307 a          580 c          572 g          287 t
ORIGIN      
        1 tcccgtcggt ccgcacgctc tcgatgccgg gaacgcgaaa ttgatcggcc accgcggcgg
       61 cgagcgccga tgcggtccaa tgtgcgacca gcaggccgag tatcccgccg gctgccgcaa
      121 tcacggcact ctcggtgagg agctgcctca ccaggcgaaa gcgtccggcg cccagcgcgg
      181 cgcgaagcgc cagctcctgc tggcgtgtgg cgccgcgcgc gagcatcagg ctggcgacat
      241 tcgcgcacgc gatcagcagt acgacgccga cgaccgacat gatcgtcaac aggcccatcc
      301 tgacccttcg cgcgacggcg tcggccatcg gcaccaggtt ggcgccgacc ccggcgtgcg
      361 aatcggggta gagccgttcg agccgcctcg tgatcgtcga caggtcgtcg cgcgcttgct
      421 cgagcgtgac gccacggcgg agccgaccca ccgtgcggag gaaaccgcgg ttgcggtttg
      481 catcgacccc cagcggctgg aagaaatcct cggaatcgcc gtcgagcgta aaacgcgatg
      541 gcatgacccc gacgatcgtg tgcggacgct cgtcgattcg caccgtctga ccgatggctg
      601 caggactgcc gccaaaatgg cgtttccaga agccatcgct caacacgacg acgtcgggcg
      661 actgcagtcg ccattcacgg aacgcgcggc cgatcgccgg ctggacaccg acgacgtcga
      721 acaggttcgc ggtgacgact tttccacgcg cggcgacaac ttcatcgccg gtgccgagcg
      781 gaaggctttg attggcgaac gccgccatcg attcaaagct ccgattctgt tcctgccagt
      841 cgatgaacgc cggatacgag acaccatcga actgcagcga tcgccgggct tccgtcgcgt
      901 agatcatgac cagccgcgac ggctcggcga gcggcaatgg ccgcagcagc gtcgcgttga
      961 cgatgctgaa gatggcgctg ttggcgccga cgccgagggc gaacgtgagg accgcggttg
     1021 cggtaaagcc tcggcttctg cgcagtacgc gtactgcgta aacgagatcc tgccagaggg
     1081 tgtcgaggaa aggcaggccg cgccggtcgc gaatcgactc gacggtttgg gtgaaaccgc
     1141 ccagctcgag cagcgccgcg cggcgggcct cgtccggagt catgccggcg cggatgttgt
     1201 cgtcgatatg gaagccaagg tgactgttga gctcgtctgc aacgtcaccg gtccgcgcgc
     1261 gtccccacag agacgcgcca agccgcagca gccaggcgcg aagcgctctc atggggcctc
     1321 ctcgcccggc gtcaggaagc gggccaggat ggccgtcgtg cgctcccact cgcgcgcttc
     1381 ccgttcaagt tgcttccggc cggcccgcgt caggcggtag aacttcgcct tgcggttgtt
     1441 gtccgacacg ccccattcgg acgcgacata gccctcctgc tcgagcttga gcagagcggg
     1501 gtagagggtt ccgtagttga gcgagaggtg gtgtgcgctg gtctgctcga tccgccgcgc
     1561 gatgccgtag ccgtgcagcg ggccgagcgt ttcgagcgtc ttaagtacca tcaatgccag
     1621 cgtgccctgc cagacgtccg ctttgtcagc catcgccatg tttccaatcc gctagccgcc
     1681 aagccagccc cgacccctgt cgtatgggat cgccatacaa agtatccgcg atctcgtatt
     1741 tgaaag
//
LOCUS       DATANS010000471         1750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_359658, whole genome shotgun sequence.
ACCESSION   DATANS010000471 DATANS010000000
VERSION     DATANS010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359658"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VHT22_06945"
     CDS             complement(<1..1028)
                     /locus_tag="VHT22_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX3137327.1"
                     /translation="MRFDKFTTKLQQALADAQSLALSNDNGFVEPAHLLASLLNQEDG
                     GTASLLSRAGVNVPALKAATEKAIARLPKVEGQGGEISVSRELGNLLNLTDKIATKRG
                     DQFIASELFLLALADDKGETGRLVKEHGLTKKALEAAIEAVRGGAAVDSQEAEGQREA
                     LKKYTIDLTERARQGKLDPVIGRDDEIRRTIQILQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGDVPESLKNKRLVSLDLGALIAGAKFRGEFEERLKSVLKEVAQDEGQTIVF
                     IDEIHTMVGAGKAEGAIDAGNMLKPALARGELHCIGATTLDEYRKYIEKDAALERRFQ
                     KILVGEPSV"
     gene            complement(1186..1638)
                     /locus_tag="VHT22_06950"
     CDS             complement(1186..1638)
                     /locus_tag="VHT22_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEX3137328.1"
                     /translation="MNAILRNLGIPAMLGMVLLLGACQNMGMMPAQQKSLYDRLGGKD
                     AIVAVVDDFVGNVAADNRINRYFAKTDIPRLKRNLVDQICAGTGGPCTYTGRDMKTAH
                     AFMGVTNADFDALVGDLVKTLDKFKVPDKEKGELLGILGPMRPSIVGH"
BASE COUNT          258 a          558 c          564 g          370 t
ORIGIN      
        1 acgctcggct cgccgacgag gatcttctgg aagcgtcgct ccagcgccgc atctttctcg
       61 atgtacttgc ggtattcgtc cagcgtggtc gcgccgatgc agtgcagctc gccgcgtgca
      121 agcgccggct tgagcatgtt gcccgcatcg atggcgcctt cggccttgcc cgcgcccacc
      181 atggtgtgga tctcgtcgat gaagacgatg gtctggcctt cgtcctgcgc cacttccttg
      241 agcacggact tgagccgctc ttcgaattcg ccgcggaact tcgcgccggc gatgagtgcg
      301 cccaggtcga gtgagacgag gcgcttgttc ttgagactct ccgggacgtc gccgttgacg
      361 atgcgctgcg cgagcccttc gacgatcgcg gtcttgccca cacccggctc gccgatcagc
      421 accgggttgt tcttggttcg ccgctgcagg atctggatcg tgcggcggat ctcgtcgtcg
      481 cgtcctatca ccgggtcgag cttgccctgg cgcgcgcgct cggtgaggtc gatcgtgtac
      541 ttcttgagcg cctcgcgctg tccttcggct tcctgcgaat cgacggccgc gccgccgcgc
      601 accgcctcga tcgccgcttc gagcgccttt ttggtcaggc cgtgctcctt aacgagcctt
      661 ccggtctcgc ccttgtcatc agcaagcgca agcaggaaca actcgctggc gatgaattga
      721 tcgccgcgct tggtggcgat tttgtcggtc aggttgagca ggttgccaag ctcgcgcgaa
      781 acgctgatct cgccgccctg cccttcgacc ttcggcaggc gggcgatcgc cttctcggtt
      841 gcggccttga gcgccggaac gttgacaccg gcgcgtgaga gcagcgaagc ggtgccgccg
      901 tcctcctgat taagcaggct cgcgagcagg tgcgccggct cgacaaagcc gttgtcgttg
      961 gagagcgcca ggctctgcgc gtccgcgagg gcctgttgca gcttggtggt gaatttgtcg
     1021 aaacgcatga ccccgccctt gcggcaatga acctagtatg aacatgggga agattgtgcc
     1081 gtattcaagc ggtctgaggg gttcaagcag ttgattcggc gcggattgcg cgcgcctgca
     1141 cgccccggcc gagtcgctcg gtccggagcg cttaagcagc aggtcttaat ggccgacgat
     1201 agacgggcgc atcggtccga ggatgcccag aagctcgcct ttttccttgt ccggcacctt
     1261 gaacttgtcc agcgtcttga ccaggtcgcc gaccagcgcg tcgaagtccg cgttggtgac
     1321 ccccatgaag gcgtgcgcgg tcttcatgtc gcggccggta taggtgcagg ggccgccggt
     1381 gccggcgcaa atctggtcga ccaggttccg cttcagtcgc ggaatgtcgg tcttggcgaa
     1441 ataacggttg atgcgattgt cggccgcgac gttgccgaca aaatcgtcga ccacggccac
     1501 gatcgcgtct ttgccgccga ggcgatcgta cagcgacttc tgctgcgccg gcatcatgcc
     1561 catgttctgg caggcgccga gcagcaggac catgcccagc atcgccggga tccccaagtt
     1621 gcgcagtatt gcgttcatct tgtctctcct ctaccgtcgt ggcagcgcgg gagcgctgta
     1681 ccggccatat acgatcagac gaggctgccg gatgcgggtc ccgacggctg tgttaggctt
     1741 cgccgtcgcg
//
LOCUS       DATANS010000472         6445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_319493, whole genome shotgun sequence.
ACCESSION   DATANS010000472 DATANS010000000
VERSION     DATANS010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319493"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..774
                     /locus_tag="VHT22_06955"
     CDS             10..774
                     /locus_tag="VHT22_06955"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3137329.1"
                     /translation="MIHARGVAKSYQTGDGEVESLRPLEFEVGEGEFVSVVGPSGCGK
                     STLLKLIAGLLPASSGSIEIGGASVDGPPPGIGIVFQSHVLLPWRTVLANILLQAEVR
                     GLPREQSLVRARQLVAMVGLEGFENKFPWQLSGGMQQRASICRALVHDPAILLMDEPF
                     GALDAMTREKMNLELQRIWAAAKKTVLLITHSIPESIFLSDRVLVMSERPGSIAAIYD
                     VALPRPRTLAMMGSPEFAVLAQTIRAHFYSKGLLDV"
     gene            808..2259
                     /locus_tag="VHT22_06960"
     CDS             808..2259
                     /locus_tag="VHT22_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012067535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase"
                     /protein_id="HEX3137330.1"
                     /translation="MQPTFSIVAIELYERSVRLRLPFRFGAATVTEAPQAFVRARIRL
                     AGGARNVDTTGAAAELMIPKWFDKSPQRSNADNIADLRASLAAAAGAYTSQRRPSTAF
                     GHAAEHYHALIDSGAGAGLNPLTASYGAALIDRALLDALCRALDVSFADAIRANLAGI
                     DARLTPDLAGFDLGRFLRTLKATPAIAARHTVGILDPIDAGDDLQRPNDSQPATLGEV
                     IATYGNRYFKIKLGGNAESDVARLLRIATVLDRLPWYAVTLDGNEQFETVAAVDDFWR
                     RVGATRSLDRLAAVALYLEQPLPRATTLVTDVSTLDSSLPLLIDESDATLEAFPAARR
                     YGYRGVSSKSCKGFYKSLLNAARCAQANALPGPRTFLSGEDLTAQAGLAVQQDLALVG
                     LLGLTHVERNGHHYVDGFAGQRAGASEQQAFLAAYPDLYRDDGGNVRLAIRDGMLDLS
                     SLGAPGFGSRAEPDWTSLAPLLADPPIASSIAR"
     gene            2294..3460
                     /locus_tag="VHT22_06965"
     CDS             2294..3460
                     /locus_tag="VHT22_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3137331.1"
                     /translation="METVRLGIIMNGVTGRMGTNQHLVRSICAIRAQGGVALADGRRV
                     MPDPILVGRNRDKVEQLAREHGIARWTTDLAAALADRDDTLYFDSASTGLRPQLIEQA
                     IAAGKHIYTEKPVAPTLAQALSLCAEARRAGVKHGVVQDKLWLPGLLKLRMLIASGFF
                     GRILSVRGEFGYWVFEGDWQPAQRPSWNYRKEDDGGIIIDMLCHWRYVLDNLFGKVKS
                     VSCLGATHIPKRWDERGRPYEATADDAAYATFELSGTGGPVIAQINSSWTTRVRRDDL
                     VTFHVDGTLGSAVAGLTDCWTQARANTPKPVWNPDVPQPIDFFAGWQKVPNNQVYDNA
                     FKVEWELFIRHLFEDTPFQWDLLEGAKGVQLAELGLKSWAERRWIDVPELEVQR"
     gene            3466..4635
                     /locus_tag="VHT22_06970"
     CDS             3466..4635
                     /locus_tag="VHT22_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX3137332.1"
                     /translation="MQRIRLPRGDGSLHEYAMRAASVWTPPAPGTGFNRVAYAAAHVV
                     ADPLSTADPWLQPAIDWDATLAYRDYLWSLGLGVAEAMDTAQRGMGLDWPASLELIDR
                     SLTAARATPGAVIACGAGTDHLEPRTATTVADVIAAYELQCEAVESRGGRIILMASRA
                     LAACARSPDDYGRVYEHILSGLRQPAILHWLGEMFDPALAGYWGHADLDRATEVCLDV
                     IRNNAAKIDGIKVSLLDKHREIALRRRLPAGVRMYTGDDFNFAELIAGDAAGHSDALL
                     GIFDPIAPAAAAALAALAAGNRAEFDAILAPTVPLSRHVFAAPTRFYKTGIVFLAWLN
                     GRQTHFVMVGGQQSARNLTHLVELFRLADAAGLLTDPPLARTRMQTLLALHGYDG"
     gene            4678..5532
                     /locus_tag="VHT22_06975"
     CDS             4678..5532
                     /locus_tag="VHT22_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX3137333.1"
                     /translation="MSSPDSNLLSINTATVRAQWSLPEIVAGCARRGIGRIAPWRDQV
                     AATGLKESAQRIRDAGVALSGYCRGGMFPALDRGGRRAALDENKRAVDEALVLGAPCL
                     VLVVGGLPKDSAGRPASKDLASAREMVRDGIGELLEYARPAGMPLAIEPLHPMYAADR
                     ACVNTISHANDLCDELGAGVGIAVDVYHVWWDPALRSEIERAGGHSERLLAFHICDWL
                     VPTTDLLLDRGMMGDGVIDIPLIRSWMESAGYRGAHEVEIFSAGNWWKKDPDEVLVTC
                     IERHRTVC"
     gene            5618..>6445
                     /gene="fba"
                     /locus_tag="VHT22_06980"
     CDS             5618..>6445
                     /gene="fba"
                     /locus_tag="VHT22_06980"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589515.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX3137334.1"
                     /translation="MPLVSMRQLLDHAAEHDYGLPAFNVNNLEQVQAIMSAAAETDSP
                     VIMQASAGARKYAGEIFLRHLIEAAVESYPNIPIVMHQDHGQSPAVCESAMRLGFSSV
                     MMDGSLKEDGKSIADYDYNLNVTKKVVEEAHALGVTVEGELGCLGSLETMKGDKEDGH
                     GAEGTMTREMLLTDPDQAADFVKSTQLDALAIAIGTSHGAYKFSRKPTGDILAIDRIK
                     EIHARIPNTHLVMHGSSSVPQDLQDIINKFGGKMKPTWGVPVSEIQRGIKHGVRKINI
                     "
BASE COUNT         1080 a         2170 c         2111 g         1084 t
ORIGIN      
        1 gcacggacgc tgatccatgc gcgcggcgtc gccaagtcgt atcagaccgg cgacggcgaa
       61 gtcgaatcgc tgcggccgct cgaattcgag gtcggcgaag gcgagttcgt ttcggtcgtc
      121 ggtcccagcg gctgcggtaa aagtacgctg ctcaaactga tcgcgggcct gctgcctgca
      181 tccagcggct cgatcgagat cggcggcgcg agcgtcgacg gtccgccgcc agggatcggc
      241 attgtgttcc agagccacgt actgctgccg tggcggacgg tgctggccaa catcctgctg
      301 caggcggagg tgcgaggcct gccccgcgag cagagccttg tccgcgcccg ccagttggtt
      361 gcgatggtcg gcctcgaggg tttcgagaac aagtttccgt ggcagttgtc cggcggcatg
      421 cagcaacgcg cctcgatctg ccgcgcactg gtgcacgatc cggcgatcct gctgatggac
      481 gagcccttcg gcgccctcga cgcgatgacg cgagaaaaga tgaacctcga attgcagcgt
      541 atctgggccg cggcgaagaa gacggttctg ctgattacgc acagcattcc ggaatcgatc
      601 ttcctgtcgg accgggttct ggtgatgagc gagcgccccg gatcgatcgc cgcaatctac
      661 gacgtcgcgc taccgcgccc gcgcacgctg gcgatgatgg gaagcccgga attcgccgtt
      721 cttgcgcaga cgatccgggc gcacttctat tcgaaagggc tgctcgacgt ctaggccgtt
      781 ggcggtcgac gcgagccctc gcctttcgtg cagccgacct tcagcatcgt cgcaatcgag
      841 ctgtacgagc ggtcggttcg cctgcgcctg ccgttccgct tcggcgccgc gaccgtgaca
      901 gaggcgccgc aggctttcgt ccgcgcgcgc atacgccttg cagggggcgc gcggaacgta
      961 gatacgaccg gcgccgccgc cgagctgatg attcccaaat ggttcgacaa gtcgccgcag
     1021 cggtcgaatg ccgacaacat cgccgatttg agagcgtcgc tggccgccgc ggccggcgcc
     1081 tacacgtcgc agcggcgtcc atcgaccgca ttcggtcacg ccgccgagca ttatcacgcg
     1141 ctcatcgaca gcggagcagg cgcgggactc aacccgctga cggcaagcta tggagcggca
     1201 ctgatcgacc gcgcgctgct cgatgcgctt tgccgggcac tcgatgtttc atttgcagac
     1261 gccatccgcg ccaatcttgc cggcatcgac gcgcgcctga ctcccgatct cgccgggttc
     1321 gacctcggcc gatttctgcg cacccttaaa gcgacgccgg cgattgccgc gcggcacacg
     1381 gtcggcatcc tcgacccgat cgacgccggc gacgatttgc agcgcccgaa cgacagccag
     1441 ccggcgacgc tcggcgaagt catcgccaca tacggcaatc gctatttcaa gatcaagctc
     1501 ggcggcaacg ccgagagcga cgttgcgcga cttctgcgga tcgcgacggt cctcgatcgc
     1561 ctgccctggt acgcggtgac gctcgacggc aacgaacaat tcgaaaccgt ggcggccgtc
     1621 gacgatttct ggcgccgcgt cggcgcgacg cgctcactcg atcgccttgc cgccgtcgcg
     1681 ctttacctcg agcagccgct gccgcgcgcc acgacgctgg tgaccgacgt gtcgacgctg
     1741 gattcgtcgc tgccgctgct gatcgacgaa tccgatgcga cgctcgaagc tttcccggcc
     1801 gcgcggcggt acggctatcg gggcgtctcc agcaagagct gcaagggatt ctacaaatcg
     1861 ctgctcaacg ccgcgcgctg cgcgcaggcg aacgccctcc ccggcccgag gacttttctg
     1921 tccggcgagg acctgaccgc gcaggccggg cttgccgtcc agcaggacct ggcgctggtc
     1981 ggcctgctcg gtctcacgca tgtcgagcgc aatggtcatc actacgtcga cggcttcgcc
     2041 ggccaacgcg ccggcgcgtc cgagcaacag gcgttcctcg ctgcgtaccc ggacctctac
     2101 cgcgacgacg gcggcaatgt ccgtctggcg atacgcgacg gaatgctcga cttgagctcg
     2161 ctgggcgcgc ccggcttcgg ctcgcgcgcc gagcccgact ggacgtcgct ggcgccgctg
     2221 ctggcggatc cgccgatcgc atcttcaatt gcgcgatgaa cggcgcagaa cctcagtctc
     2281 ggggggattg aacgtggaaa ccgtacgact cggcatcatc atgaacggcg tcaccggccg
     2341 gatgggcacc aaccagcatc tcgtacgctc gatttgtgca atccgcgcac aaggcggtgt
     2401 cgcgctggcc gatggccgcc gcgtcatgcc cgatccgatc ctggtaggac gcaatcgcga
     2461 caaggtcgag cagctcgcgc gcgagcacgg catcgcgcgc tggacgaccg acctcgccgc
     2521 ggcgctggcc gatcgcgacg acacgcttta tttcgattca gcctcgaccg gcttgcggcc
     2581 gcagctgatc gagcaggcga tcgccgccgg caagcacatc tacaccgaaa agccggtggc
     2641 gccgacgctg gctcaggcac tctcgctctg cgctgaggcg cgccgcgccg gcgtcaagca
     2701 cggtgtcgtg caggacaagc tctggctgcc gggtttgctc aagctccgga tgctgatcgc
     2761 gtccggtttt ttcggacgca tcctgtcggt ccgcggcgag tttggctact gggtgttcga
     2821 aggagactgg cagccggcgc agcgtccgtc ctggaactat cgcaaggaag acgacggcgg
     2881 gatcatcatc gacatgctct gccactggcg ctacgtgctc gacaacctgt tcggcaaggt
     2941 caagagcgtc tcttgcctcg gcgcgaccca tatcccgaag cgctgggacg aacgcggacg
     3001 cccgtacgaa gcgacggccg acgacgccgc ttatgcgacg ttcgagctct ccggcaccgg
     3061 cggtccggtg atcgcgcaga tcaacagctc gtggaccacg cgcgtccgac gcgacgatct
     3121 ggtcaccttt cacgtcgacg gcacgctcgg ctccgcggtc gcgggcctga ccgactgctg
     3181 gacgcaggcg cgcgccaata cgccgaagcc ggtatggaac ccggacgtgc cgcagcccat
     3241 cgattttttc gccggctggc agaaggttcc gaacaaccag gtctacgaca acgcgttcaa
     3301 ggtcgaatgg gagctgttca tccgccacct gttcgaagac acgccgttcc agtgggacct
     3361 gctcgaaggc gccaagggtg tgcagctggc cgagctcggc ctcaaaagct gggccgagcg
     3421 ccgctggatc gacgtgcccg agctcgaggt ccagcgttga gcgcgatgca gcgcatccgg
     3481 ctgccgcgcg gcgacggctc gctgcacgag tacgcgatgc gtgccgcatc ggtgtggaca
     3541 ccgccggcgc cgggcacggg cttcaatcgc gtcgcctacg cggccgcgca cgtcgtcgcc
     3601 gatccgttgt cgacggccga tccctggctg caaccggcga tcgactggga tgcaacgctc
     3661 gcttaccggg attatctgtg gtcgctgggc ctcggcgtcg ccgaggcgat ggataccgca
     3721 cagcggggga tggggctcga ctggccggcc tcgctcgagc tgatcgaccg gtcgctgacc
     3781 gcggcgcggg ccacgcctgg tgcagtgatc gcttgcggcg ccgggaccga ccatctggag
     3841 ccgcgtaccg cgacgacggt ggccgacgtc atcgccgcgt acgaattgca gtgcgaagcc
     3901 gtcgaaagcc gcggcggccg catcattttg atggcgagtc gtgcgctggc cgcgtgtgcg
     3961 cgctccccgg acgactacgg tcgggtgtac gagcacattc tgagcgggtt gcgccagccg
     4021 gcaatcctgc actggctggg cgagatgttc gaccccgcgc tcgccggcta ctggggccat
     4081 gcggatctgg accgcgccac cgaagtctgt ctcgacgtga tccggaacaa tgcggccaag
     4141 atcgacggta tcaaggtttc gctgctggac aagcaccgcg aaatcgcgct gcgccggcgc
     4201 ctgcccgctg gcgtgcggat gtacaccggc gacgatttca acttcgccga attgatcgcg
     4261 ggcgacgcgg ccggccactc ggatgcgctg ctcggcatct tcgacccgat cgcgcctgcg
     4321 gccgcggcgg cgctggccgc gctggccgcg gggaatcgcg cggaattcga tgcgatcctc
     4381 gcgccgaccg tgcccttgtc gcgccacgtg ttcgccgcgc cgacgcgctt ctacaagacg
     4441 ggcatcgtct ttctcgcctg gctcaacggc aggcagacgc atttcgtcat ggtcgggggt
     4501 cagcaaagcg cgcgcaatct cacgcacctg gtcgagcttt tccgccttgc cgacgccgcg
     4561 ggactcctca ccgatccgcc gctcgcgcgt acccggatgc agaccttgct cgcactgcac
     4621 ggttacgatg gctgaccgga ccccggcgtt cgatcgccgt ttcggacgca tcggacgatg
     4681 agctcgcccg attcgaacct gctttcgatc aacaccgcga cggtgcgcgc gcagtggagc
     4741 ctgcccgaga tcgtcgccgg ttgcgcgcgc cgcggtatcg gccgcatcgc gccttggcgc
     4801 gaccaggtcg ctgccaccgg cctgaaggaa tcggcccagc gcatacgcga tgccggtgtt
     4861 gcgttgtccg gctattgccg gggcggaatg tttccggcgc tggatcgcgg cggcaggcgc
     4921 gccgcgctcg atgaaaacaa gcgcgcggtc gacgaggcgc tcgtcctcgg cgcgccttgt
     4981 ctggttctgg tcgtcggcgg cctgccgaag gacagcgccg gccggccggc gtcgaaggat
     5041 ctggctagcg cgcgcgaaat ggtgcgcgac ggaatcggcg agttgctcga gtacgctcgc
     5101 cccgccggca tgccgctggc gatcgagccc ttgcacccga tgtatgcggc cgaccgcgcc
     5161 tgcgtcaaca cgatctcgca cgccaatgac ctgtgcgacg agctgggcgc aggcgtcggc
     5221 atcgccgtcg acgtctacca cgtctggtgg gacccggcgc ttcgaagcga aatcgagcgc
     5281 gccggcgggc actcggagcg cctgctcgcg ttccacatct gcgactggct ggtaccgacc
     5341 accgacctcc tgctcgatcg cgggatgatg ggcgacggcg tgatcgacat tccgctgatc
     5401 cggtcctgga tggagtcggc cggctaccgc ggtgctcacg aagtggagat cttctcggcc
     5461 ggcaactggt ggaagaaaga ccccgacgag gtgctggtca cctgcatcga acggcaccgg
     5521 accgtttgct gaactgctcg gccgggcggg gctccggcat gacggaatcc gcggtaaaat
     5581 aatcgatcca acggaacgtt cctttcagga gtccgccatg ccactcgtct ccatgcgcca
     5641 gctgctcgac cacgctgccg agcacgatta cggactgccc gctttcaacg tcaacaacct
     5701 ggagcaggtc caggcaatca tgtctgccgc ggccgaaacc gacagcccgg tgatcatgca
     5761 ggcgtcggcc ggagcgcgca aatatgccgg agagattttc ctgcgccacc tgatcgaagc
     5821 cgctgtcgag agctatccga acatccctat tgtcatgcac caggatcacg gccagtcgcc
     5881 ggcggtgtgc gaatccgcga tgcggctggg cttttccagc gtgatgatgg atggctcgct
     5941 caaggaggac ggcaagtcga tcgccgacta cgactacaac ctcaacgtga cgaagaaggt
     6001 cgtcgaggaa gcccatgcgc tgggcgtcac cgtcgaaggc gagctcggct gtctcggttc
     6061 actcgaaacg atgaaaggcg acaaggagga cggacacggc gccgaaggca cgatgacccg
     6121 cgaaatgctg ctgactgatc ccgatcaggc ggccgatttc gtcaagtcga cccaactcga
     6181 cgcgctggcg atcgcgatcg gcaccagcca cggtgcatac aagttttcgc gcaagcccac
     6241 cggcgacatc ctggcgatcg atcgcatcaa ggaaatccac gcccgcattc ccaataccca
     6301 cctggtgatg cacggctcgt cgtcggtgcc gcaggacctg caggacatca tcaacaagtt
     6361 cggcggcaag atgaagccga cctggggcgt gccggtctcc gaaatccagc gcggcatcaa
     6421 gcatggcgtg cgcaagatca acatc
//
LOCUS       DATANS010000473         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_697526, whole genome shotgun sequence.
ACCESSION   DATANS010000473 DATANS010000000
VERSION     DATANS010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697526"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VHT22_06985"
     CDS             complement(<1..309)
                     /locus_tag="VHT22_06985"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3137335.1"
                     /translation="MRLFLNQASPYARLARVVVHEKCLTDQVELVWTDPWASPPELLA
                     VNPAAKVPALVTDGGQAIVDSACICLFLDAAGQGATLFPKGIPALAKYGLGRSVIDAA
                     F"
     gene            complement(329..1513)
                     /locus_tag="VHT22_06990"
     CDS             complement(329..1513)
                     /locus_tag="VHT22_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HEX3137336.1"
                     /translation="MAVMNHVSGNQVAGAGMLALAVAMGIGRFAFTPLLPLMQDEYGL
                     TVAGGTWLATANYAGYLAGALVALAVRVPAPAAIVGGMVAIGLATLGMGYTHDYPLWL
                     ALRALAGMASAWVMIYVSSWSLERLVAAGSPALNGRIFAGVGLGIAGAGLATLAMMHW
                     RWSAGAAWIALGAGSLVVVVCVLPVFLVRIPGAARAAGAAPRQPIRWNGESMRLVGCF
                     ATSGFGYIIPATFLPAMAKQVIYDPAIFGWSWPVFGAAALLSTLAVSALRPVGNRRLW
                     AASQLLMAAGVALPALWHSMTAIILAGLLVGGTFMVITMAAVQAARAIAGPQPVGLLS
                     AMTAAFAIGQLLGPLSVRALLDTGGGLASALLIASGVLAAGAYALNGFTPAGRGLGPQ
                     QI"
BASE COUNT          318 a          561 c          477 g          217 t
ORIGIN      
        1 gaacgcggca tcgatcaccg agcgcccgag gccgtacttc gccagcgccg gaatcccttt
       61 gggaaagagc gtggcgccct gccccgctgc atcgagaaac aggcagatgc acgcgctgtc
      121 gacgatagcc tgcccgccgt ctgtgaccag tgccgggact ttcgccgcgg gattgaccgc
      181 gagcaattcc ggcggcgacg cccatggatc ggtccatacc agttccacct gatcggtgag
      241 acatttttcg tgcaccacca cccgcgcgag ccgcgcgtac ggagaagcct ggtttaaaaa
      301 tagccgcatg caagtgctcc ggtaattttc aaatctgttg agggccgagg ccgcgtccgg
      361 cgggtgtgaa tccattcaac gcgtaggcgc cggcggcgag cacgccgctg gcaatcagca
      421 gcgcggacgc aaggccgccg ccggtatcca gcagcgcgcg cacgctcagc gggcccagca
      481 gctggccgat cgcgaacgcc gcggtcatcg cgctcagcag gccaacgggt tgcggtcccg
      541 cgatcgctct ggcggcctgc acggctgcca tcgtgatcac catgaatgtg ccgccgacga
      601 gcagtcccgc caggataatc gccgtcattg aatgccacag cgcgggcaac gccacgccgg
      661 cggccatgag caactggctg gcggcccata agcgacgatt gccgaccgga cgaagtgcgc
      721 tcacggccag agtcgagagt agcgcggcgg cgccgaatac aggccacgac cacccgaata
      781 tcgccgggtc gtaaatgacc tgcttcgcca tcgcgggcag aaacgtcgcg ggaatgatgt
      841 agccgaatcc gctcgtcgca aagcagccga cgaggcgcat cgactcgccg ttccagcgta
      901 tcggttggcg cggcgcggct cccgcggcgc gcgcggcccc cggaatgcgc acgagaaaaa
      961 caggcagcac gcacacgacg acgaccagcg agcccgcgcc caacgcgatc cacgccgccc
     1021 ccgcgctcca gcgccagtgc atcatcgcga gcgtcgcgag acccgcaccc gcgatgccga
     1081 gccccacgcc cgcgaatatg cgcccgttca atgccgggct gcccgccgcc acgaggcgtt
     1141 cgagcgacca cgacgacaca taaatcatca cccacgcgct ggccatgccg gcgagcgcgc
     1201 gcagcgccag ccacagcgga taatcgtggg tgtaccccat gccgagcgtc gccaggccga
     1261 tcgcgaccat gccgccgacg atcgccgccg gcgcgggaac ccgcaccgcc aaagcgacca
     1321 gcgcacccgc gagatacccg gcgtaattgg cggtcgcaag ccaggtgccg cccgcgaccg
     1381 tcaacccgta ctcgtcctgc atcaggggca gcagcggcgt gaacgcaaac cgccctattc
     1441 ccatcgcaac tgccagtgcg agcatgcccg caccggcaac ctggtttccc gacacgtgat
     1501 tcatgaccgc catcttagga ttgaccatcc ataattaaaa atgaataatt atgatcaatt
     1561 ccatctttaa tcg
//
LOCUS       DATANS010000474         2361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_359831, whole genome shotgun sequence.
ACCESSION   DATANS010000474 DATANS010000000
VERSION     DATANS010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359831"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /gene="hflX"
                     /locus_tag="VHT22_06995"
     CDS             complement(<1..1162)
                     /gene="hflX"
                     /locus_tag="VHT22_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX3137337.1"
                     /translation="MFERPRSGERALLVRIGLGGPPGKDELSEFEALARSAGADVLRV
                     VTGTRRAPDPRLYIGSGKAEEIRGLVAELGADLVVFDHALSPSQERNLEALLTCRVLD
                     RTGLILDIFAQRARSFEGQLQVELAQLKHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLGGRIKTLNARLDKVVTQRETTRRERKRAEIPTVALVGYTNAGKTTLFNVLT
                     RADAEASNALFVTLDPVAAAAGTAAPQTFAESRNRDAQPIKDLDFRRGPDAAGRVMVE
                     LPNNQVGVDIRQQGQTLVVEFLKSALPEGLRRRMDVTDFGTPIQTVTTFQAGDRVRVV
                     IEPKGAWEHSAYQSDNQFVVEVRTIKVDPSKLTQGRGFEGEKLSLNFQNIEVR"
     gene            complement(1169..1426)
                     /gene="hfq"
                     /locus_tag="VHT22_07000"
     CDS             complement(1169..1426)
                     /gene="hfq"
                     /locus_tag="VHT22_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX3137338.1"
                     /translation="MARGQSLQDPFLNTLRKERVPVSIYLVNGIKLQGQIDSFDQFVV
                     LLKNSVSQMVYKHAISTVVPARAIRLPAGEEEGAESAVTTE"
     gene            complement(1610..>2361)
                     /gene="miaA"
                     /locus_tag="VHT22_07005"
     CDS             complement(1610..>2361)
                     /gene="miaA"
                     /locus_tag="VHT22_07005"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX3137339.1"
                     /translation="PHHLVDILDPLQAYSAGQFVRDARQAIDEIRARGKLPLLVGGTM
                     LYFRALRRGLADMPAANPVVRAAIDAEAALRGWPALHAELAALDPVAAKRIQRNDAQR
                     IQRALEVHRLTGKTLTELHATPTTDADLTFAAYAWVPSDRERLYAAIEQRFHAMMHAG
                     LLTEVRKLYARGDLNADLPAIRAVGYRQLWEHLSGRDTLDSAIQRAIFATRHLARRQL
                     IWLRAEPEIRWLDALDSSAAAHMEHAITQLQ"
BASE COUNT          392 a          767 c          753 g          449 t
ORIGIN      
        1 cgcgcacctc gatgttctgg aagttcagcg agagcttctc gccttcgaag ccgcggccct
       61 gcgtgagctt ggacgggtcg accttgatgg tgcgcacttc cacgacgaac tggttgtcgg
      121 attggtacgc gctgtgctcc caggcgccct tgggctcgat caccacgcgc acgcggtcac
      181 cggcctggaa ggtggtgacg gtctggatcg gcgtgccgaa gtcggtcacg tccatccggc
      241 ggcgcaggcc ctcgggcagc gccgacttca ggaattcgac gaccagggtc tggccctgct
      301 ggcggatgtc gacgccgacc tggttgttcg gcagttcgac catcacgcgg ccggccgcat
      361 cgggcccacg gcggaagtcg aggtccttga tcggctgggc gtcgcggttg cggctctcgg
      421 cgaaggtctg cggcgccgcg gtgcccgcgg cggcggccac cgggtcgagc gtcacgaaca
      481 gcgcgttgga cgcctccgcg tccgcccgcg tcagcacgtt gaagagggtc gtcttgccgg
      541 cgttggtgta gccgaccagc gcgacggtgg gaatctcggc gcgcttgcgt tcgcggcgcg
      601 tggtttcgcg ctgagtgacg accttgtcga gacgagcgtt gagcgtcttg atgcgaccgc
      661 cgagcaagcg ccggtcggtt tccagctgcg tttcgccggg accgcgcaga ccgataccgc
      721 ccttctgccg ctcgaggtgc gtccagcctc gcaccagtcg cgtcgcgagg tgcttcagct
      781 gcgcaagctc cacctgcaac tggccttcga agctgcgcgc gcgctgcgcg aagatgtcga
      841 gaatgagacc ggtgcgatcg agcacgcggc acgtgaggag agcctcgaga ttgcgttcct
      901 ggctcggtga cagcgcatga tcgaacacga cgagatctgc gccgagttcg gcgacgagcc
      961 cgcggatctc ctccgcctta ccgctgccga tatacagccg cgggtccggc gcgcgccgcg
     1021 tgccagttac gacgcgcagc acatccgccc cagcagagcg cgcgagcgct tcgaactcgc
     1081 tcagctcgtc tttgccggga ggacctccca gcccaatgcg caccaggaga gcacgttcgc
     1141 cactgcgggg acgctcaaac aacgtcgttc attctgtggt gacggccgac tccgcgcctt
     1201 cttcctcgcc cgccggcaag cgaatcgctc gcgccgggac gacagtggaa atcgcgtgct
     1261 tgtagaccat ctggctgacg ctgttcttca gcaggaccac gaactggtcg aacgaatcga
     1321 tctggccctg cagcttgatg ccgttcacca ggtaaatgga gacaggtaca cgctccttgc
     1381 gcagggtatt caggaacgga tcctgtaatg attgccctct ggccatcatt tatctccttg
     1441 ttatcacttt gaattcttgg ttgagacgct gccaggccgc tgcttccctg cgcaccgaca
     1501 gcgggcggca cctctccggt gcctcgttgc agtgccgcag tctaacacag cgaatactcg
     1561 ctcgaagccg cctttcgtct aatacggagc ccactttgtc gtgagtcgct tactgcagtt
     1621 gcgttatcgc gtgctccatg tgggcagcag cgctggaatc aagagcgtcg agccaccgaa
     1681 tttcaggctc ggcgcgcagc cagatcaatt gccgtcgcgc cagatgtcgc gttgcgaaaa
     1741 ttgcccgctg tatcgctgaa tccaaagtgt ctcgacctga caaatgttcc cagagctggc
     1801 ggtatccgac agcgcgtatc gcgggcaaat ccgcattcaa atcgccgcgc gcatacagct
     1861 tgcgaacttc tgtgagcaga ccggcatgca tcatcgcgtg gaaccgctgt tcgatcgctg
     1921 cgtacagccg ctcgcgatcg ctcggtaccc acgcataggc agcgaacgtc aaatctgcat
     1981 cggtcgtcgg cgtcgcgtgc aactcggtca gtgtcttgcc cgtcagccga tgcacttcca
     2041 gtgcgcgctg aatacgttgc gcatcgttgc gttggatgcg cttggcggca accggatcga
     2101 gcgcggcgag ctcggcgtgc agtgcaggcc agccgcgcag cgcagcttcg gcgtcgattg
     2161 ctgctcgcac caccgggttg gccgccggca tatccgccaa tccacggcgc aatgcacgga
     2221 agtagagcat cgtgcccccg accaggagcg gcagcttgcc gcgagcacgg atctcatcga
     2281 ttgcctgccg cgcgtcgcgc acgaactgcc cggctgagta cgcttgcagc ggatccagga
     2341 tgtcgaccag gtgatgcgga t
//
LOCUS       DATANS010000475         3068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_359987, whole genome shotgun sequence.
ACCESSION   DATANS010000475 DATANS010000000
VERSION     DATANS010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359987"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VHT22_07010"
     CDS             <1..468
                     /locus_tag="VHT22_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012212969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEX3137340.1"
                     /translation="LQGRQQQRLDRDVGDFVVKRSDGLFAYQLAVVVDDALQGITDVV
                     RGADLLASTPRQIWLQRQLGFATPAYLHHPVAIDRSGRKLSKETGAAQLPDDPLPALW
                     RAWRFLGQGAPEHRPPSVAAFWRLAHRAWDARALPPVAMLPADADAASQPFAR"
     gene            576..1142
                     /locus_tag="VHT22_07015"
     CDS             576..1142
                     /locus_tag="VHT22_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3137341.1"
                     /translation="MTTLVVVRKNKEIAIAADSLTTFGDTRLSAYFDRAYEKIVHHRG
                     TYIGLCGSAAHQLVFESLLAKHEDLDFSTRRAIFETFRKLHPILKEQHFLNPKEEEDD
                     PYESTQITALIANANGIFGVYSMREVFEYNHFWAVGSGREFALGAMHSMYGRLRSAEA
                     VAKVGIDAGTTFDKNSALPMTIYTLPLR"
     gene            1139..1771
                     /locus_tag="VHT22_07020"
     CDS             1139..1771
                     /locus_tag="VHT22_07020"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEX3137342.1"
                     /translation="MSLYRPAAFASDDQAAIARLFEEYPFATLITAGPDEPQISHLPL
                     LHESEPAPHGSLIGHMARANPHWSHFGSGASLAVFHGPHAYVSPSWYAQPAIQVPTWN
                     YAVVHVRGRAEIVADRTATLATLQKMVARFEGSRPAPWRLQLEGARLDAMVGAIVAFR
                     IDIERVDAKFKLSQNRDEADRRRVATALRSEAYADATATADWMDREAGSE"
     gene            1752..2162
                     /locus_tag="VHT22_07025"
     CDS             1752..2162
                     /locus_tag="VHT22_07025"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEX3137343.1"
                     /translation="MRPAASDPVAPARVRFDKWLWAARFYRTRSLAALAITAGHVRVE
                     DQRIKPSRVAATGERVVLRKDALVWNVVVTALSDRRGNAADAAGLYREAPESIAARAE
                     EVARRKAAAAAAPTRLGRPTKRERRKLKAFFEES"
     gene            complement(2292..>3068)
                     /locus_tag="VHT22_07030"
     CDS             complement(2292..>3068)
                     /locus_tag="VHT22_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137344.1"
                     /translation="VARLKVIKQLLSEGWRAGAVVPLAEPALQSMLGIAVDAPAPLPD
                     EIETAVTLLGEHRVGELQNHLSKLLVGQGLRRFLEQTLIPLNEAVHERVVRGEMQNFQ
                     ELRFADLAQRLLRDVTRLVRPTRDARQILLATPPNDPNQLGLAILELLLFTEGINCLT
                     LGSGVPPQEVAGAARAYKVCLAILLFDRGISGKIAGQEIRSLRAALPEDLPLVVSGRA
                     VNLLAKPIADVRTAADFSSVMVAMRELGAVGGVAQVPQRA"
BASE COUNT          526 a         1028 c         1023 g          491 t
ORIGIN      
        1 ctccagggca gacagcagca acgtctcgat cgcgatgtcg gcgatttcgt cgtcaagcgc
       61 tccgacggct tgttcgccta ccagctcgcc gtcgtcgtcg atgatgcact tcagggaatc
      121 accgatgtcg tgcgcggcgc cgacctgctc gcatcgacgc cgcggcagat ctggctgcag
      181 cgtcagctcg gattcgcgac gccggcttat ctgcatcatc cggttgccat cgaccggtcc
      241 gggcgaaagc tttcgaagga aaccggcgcg gcgcagctgc ccgacgatcc gctgccggcc
      301 ttgtggcgcg catggcggtt tctcggacaa ggcgctcccg agcaccgtcc gccatcggtg
      361 gcagcgttct ggcgcttggc gcaccgcgcc tgggatgcgc gcgcgctgcc tccggtggcg
      421 atgctgccgg ccgatgcgga cgcggcaagt cagccgttcg ctcgatagcg gcgctcggcg
      481 accgctgccc gggccgcaac ggtcgccgcg ttataatcgg gccatgcgcg gtcgctttcc
      541 gcggcgcggc gcttgccttt cgatccgatc cagtcatgac cacgctcgtc gtcgtccgca
      601 agaacaagga gattgccatc gccgccgact cgctgaccac gttcggcgac acgcggctgt
      661 ctgcgtattt cgaccgcgcc tacgaaaaga tcgtccatca tcgcggcacc tacatcggct
      721 tgtgcggcag cgccgcgcac cagctggtgt tcgaaagcct gctggcgaaa cacgaagacc
      781 tcgacttttc gacgcggcgc gcgatattcg aaaccttccg caagctgcac ccgatcctca
      841 aggagcagca cttcctcaat ccgaaggagg aggaggacga tccgtacgaa tcgacgcaga
      901 tcacggcgct gatcgccaat gcaaacggca ttttcggcgt ttactcgatg cgtgaggtct
      961 tcgagtacaa ccacttctgg gcagtgggct cggggcgcga attcgcgctc ggcgcgatgc
     1021 acagcatgta cggacgactg cgctccgccg aggccgtcgc caaggtggga atcgacgcgg
     1081 gcacgacctt cgataagaat tccgcactgc cgatgacgat ctatacgttg ccgctgcgat
     1141 gagcctgtac cggccggcgg cgtttgcgag cgatgatcaa gcggcgatcg cgcggctctt
     1201 cgaagagtac ccgttcgcca cgctgatcac cgccgggcct gacgagccgc agataagcca
     1261 cctgccgctg ctccacgaga gcgagccagc accgcacggc agcctgatcg gccacatggc
     1321 gcgcgcgaat ccgcattgga gtcatttcgg cagcggcgcg tcgctggctg ttttccacgg
     1381 cccgcacgcg tatgtgtcgc cgtcctggta tgcgcagccg gcgatacagg taccgacgtg
     1441 gaactacgcg gtcgtccacg tccgcggtcg cgccgagatc gtcgccgacc gcacggcgac
     1501 cctggcgacc ttgcagaaaa tggtcgcgcg gttcgagggt agccggcctg cgccgtggcg
     1561 gctgcaactc gagggcgcgc ggctcgatgc gatggtcggc gcgatcgtcg cattccgcat
     1621 cgacatcgag cgcgtcgatg ccaaattcaa gctgtcgcag aacagggacg aagcggaccg
     1681 ccggcgggtg gcgacggcgc tgcgaagcga agcctatgct gacgcgacgg ccacggcgga
     1741 ctggatggat cgtgaggccg gcagcgagtg acccggtcgc gccggcgcgc gtccgcttcg
     1801 acaagtggct atgggcggcg cgcttttacc gcacgcggag cctcgcggcg ctggcgatca
     1861 cggcaggaca cgtgcgcgtc gaggatcaac ggatcaagcc ttcgcgcgtt gccgcgaccg
     1921 gggaacgggt cgtgctgcgc aaggacgcac tggtgtggaa cgtcgtggtg acggcgcttt
     1981 cggaccggcg cggcaacgcc gctgatgctg cgggactcta ccgggaggcg ccggagagca
     2041 tcgccgcccg ggccgaggag gtcgctcgcc gcaaggccgc ggcggcggcg gcgccaacgc
     2101 gtcttggccg accgaccaaa cgcgagcgcc gcaaactcaa ggcatttttc gaggaaagct
     2161 aagcgcccgt tcggccagcg acgcctgctt gcgaacgagc ctcggctcag ccggccgact
     2221 cgcgccggca agccgggcgc ggcgatcgcc accggcgcga ttgcacgccg ggccggaccg
     2281 gaatcaacgc gtcaggcgcg ctgcggaact tgcgctaccc cgccgactgc gcccagctca
     2341 cgcatcgcga ccatcaccga actgaaatcg gcggccgttc gcacatcggc gatcggcttg
     2401 gccagcagat tgaccgcgcg gccgctgacc accagcggca gatcctcggg cagcgcagcg
     2461 cgcagcgacc ggatttcctg gcccgcgatc ttgccggaaa tgccgcgatc gaacagcaat
     2521 atcgccagac agactttgta cgcacgtgcc gcgccggcga cttcctgcgg cggtaccccg
     2581 ctgcccaggg tcaggcagtt gatgccctcg gtgaacagca gcaactcgag aatcgcgagg
     2641 ccgagttgat tggggtcgtt aggcggtgtc gccagcagta tctgccgcgc atcgcgcgtc
     2701 ggacgtacca gccgtgtgac gtcgcgcagc agccgctgcg cgagatcggc gaagcgcaac
     2761 tcctgaaaat tctgcatttc cccgcggacg acgcgctcgt gcacggcttc gttcaacgga
     2821 atcagcgttt gctcgagaaa gcggcgcagg ccctgtccga ccagcagctt ggacaggtgg
     2881 ttttgcaact cgccgacgcg atgctcgccg agcagggtga cagcggtctc gatttcatcg
     2941 ggaagcgggg cgggtgcgtc gactgcgatg cccagcatcg actgcagcgc cggttcggcc
     3001 agcggcacga cggcgccggc acgccagcct tcggacaaca actgcttgat aaccttgagc
     3061 cgcgcaac
//
LOCUS       DATANS010000476         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_739676, whole genome shotgun sequence.
ACCESSION   DATANS010000476 DATANS010000000
VERSION     DATANS010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739676"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VHT22_07035"
     CDS             complement(<1..631)
                     /locus_tag="VHT22_07035"
                     /inference="COORDINATES: protein motif:HMM:NF016827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX3137345.1"
                     /translation="MKPTLQLKLSQHLTLTPQLQQSIRLLQLSTLELNAEVERLLQEN
                     PLLEKAEDDEHVAPPPEFAQSAATSATVDEPLERADGTLQSDFDSMTSAAGDGDWGSS
                     TGGTDDDDENFSPQQVATSSLRDHLNSQLAELNLPLRDRQIVSALIDALNDDGYLTTS
                     LEELLEFFPEELGIDADELQIALRHLQNFEPAGVGARDASECLALQLKVM"
     gene            complement(774..1496)
                     /gene="lptB"
                     /locus_tag="VHT22_07040"
     CDS             complement(774..1496)
                     /gene="lptB"
                     /locus_tag="VHT22_07040"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX3137346.1"
                     /translation="MSTLSVAALNKRYKSRIVVHDVSLAVGSGEVVGLLGPNGAGKTT
                     CFYMIVGLVASDGGAIELDGDDLTRMPIHARARLGLSYLPQEASIFRKLSVADNVQAI
                     LELQGIDADEIANRLDALLEDLSISHLRDASAVSLSGGERRRVEIARALATRPRFILL
                     DEPFAGIDPIAVLDIQKIIGFLKEREIGVMITDHNVRETLGICDRAYIINEGRVLASG
                     TPDEIVYNENVRKVYLGEHFRL"
     gene            complement(1513..2193)
                     /gene="lptA"
                     /locus_tag="VHT22_07045"
     CDS             complement(1513..2193)
                     /gene="lptA"
                     /locus_tag="VHT22_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEX3137347.1"
                     /translation="MIDKTMRTLLARGLARVCCALVCLGSIASPAWAEKADRDKPIHF
                     QGDTGGGNAETKNGELVGNVVVTQGTLSIHADRITFRQNPDNSLSATAYGNPVTFREK
                     RDGVDEYYEGFAQRIVYDGQKRFVELFDNALLKKGSDQIRSNYISYDAATESFKAEGR
                     PDSGAAASGEVPLGARVRGVFQPESKDAKDGKTAKGATAAPAASAPKPDPPLPLKPDD
                     KIAPPAGK"
BASE COUNT          369 a          723 c          756 g          461 t
ORIGIN      
        1 acatcacctt gagctgcagc gccaggcact ccgaagcatc gcgggcgccg acgccggcgg
       61 gctcgaagtt ttgcaggtgc ctgagcgcga tctgcaactc gtcggcgtcg atgcccagct
      121 cctcgggaaa gaactcgaga agctcttcca gcgacgtcgt caggtagccg tcgtcattca
      181 gcgcgtcgat cagcgccgat acgatttgcc ggtcgcgcaa cggaaggttg agctcggcca
      241 gctgcgaatt gagatggtcg cgcagcgagc tggtcgcgac ctgttgcggc gagaaatttt
      301 cgtcgtcgtc gtcggttcca ccggtcgagc tgccccagtc gccgtcgccc gcagcgctgg
      361 tcatcgaatc gaaatccgac tgcagcgtgc cgtccgctcg ctccagcggt tcatcgaccg
      421 tcgccgacgt cgccgccgac tgcgcgaatt ccggcggtgg agcgacgtgc tcgtcgtcct
      481 cggctttttc gagcagcgga ttttcctgaa gcagccgctc gacctcggca ttgagctcca
      541 gcgtcgacaa ttgcagcagg cggatcgatt gctgcagctg cggcgtcagc gtcagatgct
      601 gactgagttt gagctggagc gtcggcttca ttgcgattgc acgccggcgc ggcggcagcg
      661 gggcggcagc cggtgttgca gtgcggaaac ggacgcggcc cggcctccgg cggtgcggca
      721 gggccgcgtg gccggcaggg acccacacct gcccggaact atcgtcgtca tggctaaagc
      781 cggaaatgct ctcccagata gaccttcctg acgttttcat tataaacaat ttcgtctggc
      841 gttcccgagg ccagcacccg cccctcgttg atgatgtagg cgcggtcgca tattcccagc
      901 gtttcgcgga cgttgtgatc ggtgatcatc acgccgattt cgcgctcctt cagaaagccg
      961 atgattttct ggatgtcgag caccgcgatc ggatcgatgc cggcaaacgg ctcatcgagc
     1021 agaatgaagc gcggtcttgt cgccagcgcc cgcgcgatct cgacgcgcct gcgctcgccg
     1081 ccggaaaggc tgacggcgga cgcgtcgcgc aaatgagaga tcgagaggtc ctcgagcagc
     1141 gcgtcgagac gattggcgat ttcgtcagcg tcgattccct gcagctcgag gatcgcctgc
     1201 acgttgtcgg ccactgaaag cttgcggaaa atcgacgctt cctgcggcag atacgacagg
     1261 cccaggcgtg cgcgcgcatg aataggcatt cgcgtcaggt cgtcgccgtc gagctcgatc
     1321 gcgccgccgt ccgaggcgac gagcccgacg atcatgtaga agcaggtggt cttgccggcg
     1381 ccgttcggac ccagcaaccc gacgacctcg ccgctgccga ccgccagcga gacatcgtgg
     1441 acgacgatgc gcgatttgta gcgcttgttc agcgcggcga ccgatagcgt gctcattgca
     1501 ttcgccgacg gctcatttgc cggcgggcgg tgcgatcttg tcgtcgggct tcagcggcag
     1561 cggcgggtcg ggcttggggg cggacgcggc cggcgcggcg gtcgcgccct tggcggtctt
     1621 gccgtccttg gcgtccttcg attccggctg gaatacgccg cgaacccgcg cgccgagcgg
     1681 tacttcgccg gaggcggcgg ccccgctgtc cgggcgacct tcggccttga acgactcggt
     1741 cgccgcgtcg taggaaatgt aattgctgcg aatctggtcg ctgccttttt taagcagcgc
     1801 gttgtcgaac agctcgacga accgtttctg tccatcgtag acgatgcgtt gcgcgaagcc
     1861 ctcgtaatac tcgtcgacgc cgtcgcgctt ttcgcggaat gtcaccggat tgccataggc
     1921 agtcgcggac agcgagttgt ccgggttctg gcgaaatgtg atccgatcgg cgtgaatcga
     1981 cagcgtgccc tgagtgacga cgacgttgcc gacgagctcg ccgttcttgg tctcggcgtt
     2041 gccgccgccg gtgtcgccct gaaagtggat cggtttgtcg cggtcggcct tctctgccca
     2101 cgccggcgac gcgatgctgc cgagacagac aagggcgcag caaacgcgcg cgaggccccg
     2161 ggcgagcagc gtgcgcatcg ttttatcgat catcggctat ttcttctggg gctcgatttg
     2221 tccgctgaca cgggacttaa gcttgaccgt cttgctcttg ttgtcgtatt ccatcccggt
     2281 cgcattgatt atcccacggg ggtcggcga
//
LOCUS       DATANS010000477         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_65337, whole genome shotgun sequence.
ACCESSION   DATANS010000477 DATANS010000000
VERSION     DATANS010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65337"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VHT22_07050"
     CDS             complement(<1..758)
                     /locus_tag="VHT22_07050"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011154330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX3137348.1"
                     /translation="MMIKVAINGYGRIGRNILRAHYETGRKHPLAIVAINDLGSPETN
                     AHLTRHDTVHGKFPGKVDVDGDAMVVNGDRIKVFAQRNPAELPWSALGVDVVLECTGL
                     FTTKEKASAHLKGGAKKVIISAPGGKDVDATIVYGVNHQSLKAAHTVISNASCTTNCL
                     APLVKPLHAKIGLVNGLMTTIHAYTNDQVLTDVYHEDLRRARSATHSMIPTKTGAAAA
                     VGLVLPELNGKLDGYAIRVPTINVSLVDLSFIAAR"
     gene            complement(755..1357)
                     /locus_tag="VHT22_07055"
     CDS             complement(755..1357)
                     /locus_tag="VHT22_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3137349.1"
                     /translation="MGDAPGIVIRRAAPRDAEAIARVRIDGWRTSYRGLIPAAYLDGM
                     QVDASTALWDKVLAAGPNPASVFVASHGDEVIGFAAGSLLAEPKFGLNTELTAVYLRR
                     EFQRVGLGRRLIAAVVDAQRALGAAGMIVWVIAANKPARAFYEALGAELLVEQAYQWD
                     GMDLLEAAYGWRDLDALAAACRAGQLLPETESTREDRRAG"
     gene            complement(1350..>2190)
                     /locus_tag="VHT22_07060"
     CDS             complement(1350..>2190)
                     /locus_tag="VHT22_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX3137350.1"
                     /translation="TNWSGSRPVSAHSPGNYINFGVREFAMTAIASGLTLHGGFIPYV
                     GTFLTFSDYARNAVRMAALMRIRSILVYTHDSIGLGEDGPTHQSIEHAAALRLIPHLD
                     VWRPCDTVETALAWTAALEHRHGTSCLLLTRQNVVFNPRRADQITAIRRGAYVLVDTE
                     GARATIIATGSEVALAVGAQKALADAGVPVRVVSMPCCSVFDRQDAAYRDTVLPRGLP
                     RVAVEAGVTDYWRKYVGAIGDPTGAVVGIDRFGESAPAAVLFKHFGFTVDHVVAAVRN
                     VLG"
BASE COUNT          361 a          712 c          743 g          374 t
ORIGIN      
        1 cgcgcggcga tgaacgacag gtcgacgagc gagacgttga tcgtcggaac ccggatcgca
       61 taaccgtcta gcttgccgtt gagctcgggc agcaccagcc cgacggcagc cgcggcgccg
      121 gtcttggtcg ggatcatgct gtgcgtcgcg ctgcgcgcac gccgcaggtc ctcgtggtag
      181 acgtcggtca gcacctggtc gttggtgtac gcgtggatcg tcgtcatcag cccgttgacg
      241 agaccgatct ttgcgtgcag cggcttgacc agcggagcga ggcaattggt cgtgcacgag
      301 gcgttcgaga tcaccgtatg cgccgccttc agcgactggt gattgacgcc atagacgatc
      361 gtcgcgtcga cgtctttgcc gccgggcgcc gagatgatca cctttttggc gccgcccttg
      421 aggtgagccg acgctttttc cttggtggtg aaaagccccg tgcattcgag cacgacgtcg
      481 acgcccagcg cgctccatgg cagctcggcc ggattgcgct gcgcgaaaac cttgatgcgg
      541 tcgccattga cgaccatcgc gtcgccgtcg acgtcgacct tgcccggaaa cttgccgtgc
      601 acggtgtcgt gccgggtcag atgcgcgttc gtctcggggc tgcccaggtc gttgatcgcg
      661 acgatggcca gcggatgctt gcgtccggtc tcatagtgcg cgcgaaggat attgcgaccg
      721 atgcgtccgt agccgttgat ggcaaccttg atcatcatcc tgctctcctg tcttctcttg
      781 tcgattcggt ttcgggcaat agctgtcctg cgcggcacgc cgccgccagc gcatccagat
      841 cgcgccagcc gtaggccgct tccagcaaat ccatcccatc ccattggtac gcctgctcga
      901 ccaaaagctc cgcacccagc gcttcataga aagcccgcgc cggcttgttg gcagcgatga
      961 cccagacgat catgccggcg gcgccgagag cacgctgcgc atcgacgacg gccgcgatca
     1021 ggcgccgtcc gagtccgacg cgctgaaatt cgcggcgcag atacacagcg gtgagctcgg
     1081 tgttgagtcc gaatttgggc tcggcgagca ggctgcccgc ggcgaagccg atgacctcgt
     1141 caccgtggct cgcgacaaac acactggccg ggttcggtcc cgcggcaagt accttgtccc
     1201 acagcgcggt gctggcatcg acctgcatgc cgtcgaggta ggcggccgga atcaggccgc
     1261 gatacgacgt ccgccagcca tcgatgcgca cgcgagcgat cgcctcggcg tcgcgcggcg
     1321 cggcgcggcg gatgacgatg ccgggcgcgt cacccaagga cgttgcggac ggcggcgacg
     1381 acatggtcga cggtgaatcc gaaatgcttg aacaggacgg ccgccggtgc cgattcgccg
     1441 aagcggtcga taccgacgac ggcgccggtg ggatcgccga tggctccgac gtatttgcgc
     1501 cagtagtcgg tgacgcctgc ctcgacagcg acccgcggca ggccgcgggg cagcacggtg
     1561 tcacgataag cggcgtcctg gcgatcgaag acgctgcagc atggcatcga cacgacccgc
     1621 accggcacgc cggcgtcggc gagcgccttc tgcgcgccga cggcgagcgc gacctccgag
     1681 ccggtggcga tgatggtcgc gcgcgcgcct tcggtgtcga ccaggacata ggcgccgcgg
     1741 cggatcgcgg taatctggtc ggcgcggcgc ggattgaaca cgacgttctg ccgcgtcagc
     1801 agcaggcagc tcgtgccgtg ccggtgctcg agcgccgccg tccaggccag cgccgtctcg
     1861 acggtgtcgc agggccgcca cacatcgaga tgcgggatca gccgcagcgc ggccgcatgc
     1921 tcgatcgact gatgcgtcgg tccgtcttcg ccgagcccga tcgaatcgtg cgtgtagacc
     1981 agaatcgagc gaatccgcat cagcgccgcc atgcgcacgg cgtttcgcgc gtagtcggaa
     2041 aaggtgagga aggtgccgac gtacgggatg aatccaccgt gcagggtgag gccgctggcg
     2101 atcgcggtca tcgcgaattc gcggacgcca aaattgatgt agttgcccgg agaatgcgcg
     2161 ctcaccggcc tgctgcccga ccagttggtg
//
LOCUS       DATANS010000478         1695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_779891, whole genome shotgun sequence.
ACCESSION   DATANS010000478 DATANS010000000
VERSION     DATANS010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779891"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VHT22_07065"
     CDS             <1..475
                     /locus_tag="VHT22_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137351.1"
                     /translation="DIGVARIFEVRHQWKFAELQGKRGADAIMLTFRDDYTGDRVLAF
                     VAGLGSMMQTAFNDKTEFFVLDDLDPQRLYNAARNVEIAVWKLSNTRAAQGEFFLLSN
                     ETAGPLRNLSFEREFGKIIGSLDTLSKIIADKENRTIVKVIQSVATAVFLPLKIP"
     gene            complement(498..>1695)
                     /locus_tag="VHT22_07070"
     CDS             complement(498..>1695)
                     /locus_tag="VHT22_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX3137352.1"
                     /translation="VRAAIREQSDRLAFAHSRFFTTEAAEKLADDLVAGAPGELSKVW
                     LTCGGSESVEAALKLTRQYFVESGQAQRKFVIARRQGYHGNTFGALSASGNIWRRRQF
                     EPVLIDSIQHIAPCYAYRDKAAAETPEAYGLRMANELETKIIELGTDKVAAFIAETVV
                     GATLGAVAAAPGYFKRIREICDRYGVLLILDEVMCGMGRTGTRYACEQEGIAPDMLTV
                     AKGLGAGYQPIGALLVGEKIHRAIERGGNVVMHGHTFTGHPVACAAALATQNEIRKHD
                     LLANVVRQGKALAAALTDRFGNHPNVGDIRGRGLFMALEFVADRRTKAPFDPALKINA
                     KIKEVGMENGLICYPAGGLVDGKSGDHVMIAPPFIVEAQHIAEIVDKLGRTVDAVLRD
                     AMKKAA"
BASE COUNT          285 a          542 c          546 g          322 t
ORIGIN      
        1 cgacatcggc gttgcccgca tcttcgaggt ccggcaccag tggaaattcg ccgagctcca
       61 gggcaaacgc ggcgctgatg cgatcatgct cacgtttcgc gatgactaca ccggcgaccg
      121 cgtgctcgcg ttcgtcgcgg ggctgggcag catgatgcag accgcgttca acgacaagac
      181 cgagttcttc gtgctcgacg atctcgaccc gcagcggctc tacaacgcgg cgcgcaacgt
      241 cgaaatcgcg gtgtggaaac tatcgaatac ccgcgcggcg caaggcgaat ttttcctgct
      301 gtcgaacgaa accgcggggc cgctgcgcaa cctgtcgttc gagcgcgaat tcggcaagat
      361 catcggcagc ctcgacaccc tgtcgaaaat catcgccgac aaggaaaacc gcaccatcgt
      421 caaagtgatc caaagcgtgg cgaccgcggt gttcctgccg ttgaaaattc cctagcggga
      481 acgcggttgc gcggcagtca ggccgctttc ttcatcgcgt cgcgcagcac tgcgtccacc
      541 gtgcggccga gcttgtcgac gatttccgcg atgtgctgag cctcgacgat aaacggcggc
      601 gcgatcatca cgtgatcgcc gcttttgccg tcgacgagtc cgcccgccgg ataacagatc
      661 agcccgtttt ccatgccgac ctctttgatc ttcgcgttga tcttgagcgc gggatcgaac
      721 ggtgctttgg tgcggcggtc ggcgacgaat tcgagcgcca tgaaaaggcc gcggccgcgg
      781 atgtcgccga cgtttggatg gttgccgaag cgatcggtga gggccgccgc gagcgctttg
      841 ccctggcgca ccacgttcgc aagcagatcg tgcttacgga tttcgttttg cgtcgcgagc
      901 gcggccgcgc aggcgaccgg atggccggtg aacgtgtgcc cgtgcatcac gacattgccg
      961 ccgcgttcga tcgcgcggtg aattttctcg ccgacgagca gcgcgccgat cggctggtag
     1021 ccggcgccga gccccttcgc gaccgtcagc atgtcgggtg cgatgccttc ctgctcgcat
     1081 gcatagcgcg tgccggtgcg gcccatgccg cacatcactt cgtcgaggat cagcagcacg
     1141 ccgtagcggt cgcagatttc gcggatgcgc ttgaagtagc cgggtgccgc ggcaaccgcg
     1201 ccgagcgtcg cgccgacgac cgtctcggca atgaacgcgg cgaccttgtc ggtgccgagc
     1261 tctatgattt tcgtttcgag ctcgtttgcc atgcgcagcc cgtacgcctc cggcgtttca
     1321 gcggcggctt tgtcgcggta cgcgtagcac ggcgcgatat gctggatcga gtcgatcaga
     1381 accggctcga actggcggcg ccgccagatg ttgccgctcg cggacagcgc gccaaacgtg
     1441 ttgccgtgat aaccctggcg gcgcgcgatc acgaattttc tttgcgcctg gccgctctcg
     1501 acaaaatact gacgcgtgag cttcagcgct gcttcgaccg attcggaccc gccgcaggtg
     1561 agccagactt tggaaagttc gccgggcgcg cccgccacga ggtcgtcggc gagcttttcg
     1621 gctgcttcgg tcgtgaagaa gcgcgagtgc gcgaatgcga gccggtcgga ctgctcgcgg
     1681 atcgccgcgc gcacg
//
LOCUS       DATANS010000479         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_107918, whole genome shotgun sequence.
ACCESSION   DATANS010000479 DATANS010000000
VERSION     DATANS010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107918"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VHT22_07075"
     CDS             complement(<1..450)
                     /locus_tag="VHT22_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3137353.1"
                     /translation="MAAVTLAGVRKLFGSTEVLHGVDVAVDDGEFCVLVGPSGCGKST
                     LLRMIAGLEEISDGVVTIGGRTVNTMPPKERDIAMVFQNYALYPHMTVFDNMAFSMKL
                     AGEPAAAMRKRVNDAAQILGLTEYLERYPRQLSGGQRQRVAMGRAIVR"
     gene            651..>1581
                     /locus_tag="VHT22_07080"
     CDS             651..>1581
                     /locus_tag="VHT22_07080"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3137354.1"
                     /translation="MLEPFGVDRANFPGPVVITGAGGCIGSWVLALLARAGVPACAFD
                     LSADKRRPRLLVPESDLAAIQWRTGDIADSKSVIQMLEAVRPCAVIHLAALQVPFCKA
                     DPVAGAKVNVVGTVNVFEAARQLGIKRLAYASSVAAHGAIEEGLGTMRTLYGAYKYCD
                     EQIARVYSDDWGVHSVGIRPGVVYGVGRDQGLTSTTTVAILAAAAGKSYTVPFAGGVS
                     WLYGAEVASAFIRAVSRERAGAPVFEMNGVYAPVEDGLRILKELAPSAAITCTGQPLA
                     FPMHLPDTPLREFLGDYGTMPLADGIRQTFDAFR"
BASE COUNT          261 a          515 c          510 g          295 t
ORIGIN      
        1 gcgcacgatg gcgcgaccca tcgcgacgcg ttgacgctgg ccgccggaca gctggcgcgg
       61 atagcgctcg agatactccg tgagcccgag gatctgcgcc gcatcattga cgcgtttgcg
      121 catcgcagcc gcgggctcgc ccgcgagctt catgctgaac gccatgttgt cgaagaccgt
      181 catgtgcgga tatagcgcgt agttctggaa caccatggcg atgtcgcgct ccttcggggg
      241 catggtattg accgtgcgcc cgccgatcgt gaccactcca tcggaaattt cctccagccc
      301 cgcgatcatg cgcagcagcg tggatttgcc gcagcccgag gggccgacca gcacacagaa
      361 ctcgccgtcg tcgactgcta cgtcaacgcc atgcagaacc tcggtcgagc caaataattt
      421 gcgcacgccg gccaaggtca ctgctgccac gctacccctc cacattcagc gccggattgc
      481 ggcgtttttt atccggcagg cgcgtatacg atgcatgccg ggttgagcga agcaggttag
      541 ccgcgcctgc cggagcggtc aagttatcgc gaatcgcgcc atggtggcag tcaggccagg
      601 ggttgtatga tgtatttctc gagcaaccga tatcaacggg gcatgccggc atgttagagc
      661 cttttggcgt cgatcgtgca aattttcctg gtccggtcgt gatcaccggc gctggcggct
      721 gcatcggcag ctgggtgctg gcgctgcttg cgcgcgccgg cgttcccgcc tgtgcgttcg
      781 acctctccgc tgataaacgg cgcccgcgcc tgctcgtccc cgaatccgac ctcgcggcga
      841 tccagtggcg gaccggcgac attgcggaca gcaagtcagt catccagatg ctggaagccg
      901 tgcgtccgtg cgccgtcatc cacctcgccg ctttgcaggt gccgttctgc aaggccgacc
      961 cggtcgccgg cgcgaaggtc aacgtcgtcg gcaccgtcaa tgtgttcgaa gcggcgcgcc
     1021 agctcggcat caagcggctt gcgtacgcaa gctcggtggc ggcgcacggc gcgatcgagg
     1081 aaggcctcgg caccatgcgg acactctacg gcgcatacaa gtactgcgac gaacagatcg
     1141 cccgcgtata ttcggacgac tggggcgtgc acagcgtcgg catccggcct ggtgtcgtgt
     1201 acggcgtcgg ccgcgaccag gggctgactt ccacgacgac ggtcgcgatt cttgccgcgg
     1261 ccgcgggcaa atcgtacacg gtgcctttcg ccggcggcgt atcctggttg tacggcgcgg
     1321 aggtggcaag cgcgttcatc cgcgccgttt cccgcgagcg cgccggcgca ccggttttcg
     1381 agatgaacgg agtctacgcg ccggtcgaag acggcctgcg catactcaag gagctcgcgc
     1441 cttctgctgc gatcacgtgc accggacagc cgctggcttt tccgatgcac ctgccggaca
     1501 cgccgctgcg cgaatttctc ggcgactatg gaacgatgcc gctggcggat ggcatccggc
     1561 aaacatttga cgcgtttcgc g
//
LOCUS       DATANS010000480         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_65589, whole genome shotgun sequence.
ACCESSION   DATANS010000480 DATANS010000000
VERSION     DATANS010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65589"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..929)
                     /locus_tag="VHT22_07085"
     CDS             complement(33..929)
                     /locus_tag="VHT22_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX3137355.1"
                     /translation="MRIGAIGLFAVPSAIWGSTWLAIKYQLTGVAPEVSVAYRFALAA
                     ALLAAWCLGTGRSLRFSRRDHAYLVAVGVTLFGLNYVGVYWAEHFVTSGLVAVLFSTI
                     VFLNPIGMRLFFGTPLTARTFVAAAMGVAGVALLFLPELVEARGGGTAAYGIAFGLGA
                     TIIASGGNLIAQRNQRAGIPTLAGNAWGMTYGAVVAAVAASVHGVDWTFDAHPGYLLS
                     LAYLALFGSVIAFGAYLTLLRRIGAARAAYTAVATPVIALALSTLFEGYRWTWVGALG
                     VVPAIAGNTLGLRRPPVRRKAE"
     gene            1250..>1567
                     /locus_tag="VHT22_07090"
     CDS             1250..>1567
                     /locus_tag="VHT22_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137356.1"
                     /translation="MRISTLIAAVAGLITGALMLLAAPCALALSLGAAEPQSSLGQPL
                     RVVVPIDAAKAQSLTAACVHVIADAASGSGAPRLLTGRVTIDRTAAGLQLVVTTARAV
                     NEPV"
BASE COUNT          296 a          536 c          511 g          224 t
ORIGIN      
        1 agcgtgccct ggcgagtcgc gtcgcccgtc gttcattcgg ctttccgccg cacgggtggt
       61 cggcgcagcc ccagcgtgtt gcccgcgatc gccggcacga cgcccagcgc gccgacccaa
      121 gtccagcggt agccttcgaa cagggtcgat agcgccagcg cgatcaccgg cgtcgccacc
      181 gccgtgtaag cggcgcgcgc cgcaccgatg cgtctcaaca aggtcagata cgcaccgaac
      241 gcgatgacgc tgccgaacag cgccagatag gcgagcgaca acagatagcc cgggtgcgcg
      301 tcgaacgtcc agtcgacgcc gtggaccgac gccgccacgg cggcgacgac agcgccgtaa
      361 gtcatgcccc aggcgttgcc ggccaacgtc ggtatgccgg cgcgctggtt gcgctgggcg
      421 atcaggttgc cgccgctggc gatgatggtc gcgcccagcc cgaacgcgat gccgtaagcc
      481 gcggtgccgc cgccgcgcgc ctcaacgagc tccggcagga acaacagcgc gacgccggca
      541 acacccatcg ccgcagcaac gaaagtccgc gcggtcagcg gtgtgccgaa gaacaggcgc
      601 atgccgatcg ggttcaggaa cacgatcgtc gaaaacagca ccgcgaccag gcccgaagtc
      661 acgaagtgct cggcccagta aacgccgacg taattcaagc cgaagagcgt tacgcctacc
      721 gcgaccagat atgcgtggtc ccgccgcgaa aaacgcagcg agcgtcccgt ccccagacac
      781 caggccgcca gcaaggccgc cgcgagcgcg aagcgatacg cgaccgagac ttcgggagcg
      841 acaccggtga gctgatactt gatcgcaagc caggtcgatc cccagatggc cgatggaacg
      901 gcaaacaggc cgatggctcc gattcgcatg tgcttcaacg tcggctcaac gcgaccggcc
      961 gctgcctgtg tcgatggttg tacgcaacaa gccgaatgcg acgacgccga cgtccgcgag
     1021 cgccgctcgc agcgtgtgat gcattgcatt cgtgatggcg cgacggaaca gaagatacac
     1081 ggtaacgaat ggatggcgca gcgggcgcga cccggaagct taccgcaccc caccatcgcc
     1141 ggttttcgcc tatcggcatg gaacttgctc aatccagggc gaaaaccgga ccgggtcggc
     1201 agctggcagg cgcacgaccg gaatcggtta ctttttgctc gaggctgtca tgcggatttc
     1261 gaccttgatc gctgcggtag ctggactgat caccggcgcg cttatgttgc tggcggcgcc
     1321 ctgcgcgctc gccctttcgc tcggtgctgc cgagccacaa tcgtcgcttg gccagccgct
     1381 gcgagtcgtc gtcccgatcg acgccgccaa ggctcagtcg ctgaccgccg catgcgtcca
     1441 cgtcatcgcc gacgcggcga gcggcagcgg tgcaccgcgg ctactgacgg gcagagtcac
     1501 tattgatcgc accgcggccg gactgcagct cgtggtgacc actgcgcggg cggtgaacga
     1561 gccggtg
//
LOCUS       DATANS010000481         4907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_739971, whole genome shotgun sequence.
ACCESSION   DATANS010000481 DATANS010000000
VERSION     DATANS010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739971"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VHT22_07095"
     CDS             complement(<1..301)
                     /locus_tag="VHT22_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX3137357.1"
                     /translation="MNPAKRREIYERLRQQNPHPRSELTYRTPFELLIAVVLSAQATD
                     KSVNLATAKLFQVARTPQAIVKLGEAGLIPYIQTIGLFRTKAKNVVALCRQLIAEH"
     gene            complement(298..921)
                     /locus_tag="VHT22_07100"
     CDS             complement(298..921)
                     /locus_tag="VHT22_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEX3137358.1"
                     /translation="MRNTAALADRIDDLLPQTQCGRCAHPGCRPYAEAIAAGTAPINR
                     CAPGGTATIAALAALLDVPAPSPDPAFGVAEPLTLASIDETVCIGCTLCIQACPVDAI
                     VGAAKRMHTVLADHCTGCGLCLPPCPVDCIVMTSAGRAWTEDDAKAARTRFESRKLRL
                     AQETAKRTPRPDVKTRPRVQNATPDARRQAVAAALARARARRARESR"
     gene            1053..1949
                     /locus_tag="VHT22_07105"
     CDS             1053..1949
                     /locus_tag="VHT22_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX3137359.1"
                     /translation="MRERRALRLAVALTVGFGLIEAAGGWLAGSLALLADAGHMVTDA
                     AALGIALLAQHVARLPPSRRASYGYARAEVIAAFINALLMLAVVVGISVEAVRRLLAP
                     SPVAGGMVMAIAAIGLLINMFAAWSLSHGHSLNSRAALLHVSGDMLGSVAAIVAGAVV
                     ASTGWMPIDPILSLLVAGLVLRSTWLLLKASTHVLMEGVPAHLPYEEIGRALTRVQGV
                     EAVHDLHVWQMTSERAALSAHLLIRDPQAWPQILAAAQRLLADRYQIDHVTLQPAWHA
                     PPADKRVIPLKPIAGDDKPHLH"
     gene            complement(1971..2936)
                     /gene="corA"
                     /locus_tag="VHT22_07110"
     CDS             complement(1971..2936)
                     /gene="corA"
                     /locus_tag="VHT22_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX3137360.1"
                     /translation="MLVNCVAYQEGKKLADIPKEAISDYLKRPDTFVWVALKDPPPDE
                     LEEMREEFDLPGLAVEDARHGHQRPKIEEYGDSLFAVLHAVEMRDNELHVGEIDIFVG
                     PNYVLSIRRDTERGFADVRARSEHEPDLLKHGAAYVLYALMDKVVDRYFPVLETLETE
                     LEAVEEQIFVRNAARSNIEALYDLKRKLMTLKHAVDPLMEAVGKLYGGRVPRICAGMG
                     EYFRDVFDHLQRLHASIEGIRDMQATAIQVNLGMINLNETEVTKKLAAWAAIIAVPTL
                     IAGIYGMNFKNMPEYDLQYGYQISLAVMAAIDVGLYLWFKRIRWL"
     gene            3111..4319
                     /gene="pncB"
                     /locus_tag="VHT22_07115"
     CDS             3111..4319
                     /gene="pncB"
                     /locus_tag="VHT22_07115"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011544920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX3137361.1"
                     /translation="MIIESLLDTDLYKFTMMQVVLHHFPAAQVEYRFKCRTPDVDLTP
                     YLDQIEREIIGLCALRFTHEELEYLRRWRFLKSDFVDLLGLFQLDRRFISLTPAAAPG
                     EIDITIKGPWLHTILFEVPVLAIVSEVYFRETIARPDLAEGRRRLDAKIAQINAVPDP
                     AFRIADYGTRRRFSREWQDEVVRTLKEGIGPKFVGTSNVKLAFEHNLTPLGTMAHEYM
                     QACQAVGPRLRDSQVFAFNTWAREYRGDLGIALSDICGMDAFLRDFDLFFCKLFDGVR
                     HDSGDPFEWGEKMIAHYQKMRIDPRSKVMVFSDSLNAPLAIRLFEYFKGRSQTAFGVG
                     TNLTNDLGYDALQIVIKMTRCNGQPVAKISDEPSKAMDYDPSYVAYLREVFEVPEAVA
                     TAGGDGLDAD"
     gene            complement(4316..4825)
                     /locus_tag="VHT22_07120"
     CDS             complement(4316..4825)
                     /locus_tag="VHT22_07120"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX3137362.1"
                     /translation="MHPEIIAVRADITTLTVDAIVNAANTSLLGGGGVDGAIHRAAGP
                     ELLAECRALRGCATGDAKITRGYRLPARHVIHTVGPVWHGGNAGEPQLLASCYRHSLE
                     LAVGHGLASIAFPAISCGAYGYPLAKAVPIAVATVRAFDRLDALQRVVFVCAGADVLA
                     AYRAGLAEP"
BASE COUNT          801 a         1611 c         1623 g          872 t
ORIGIN      
        1 cgtgctcggc gatcagttgc cggcatagcg cgaccacgtt cttcgccttg gtgcgaaaga
       61 gcccgattgt ctggatatac ggaatcagcc ctgcttcgcc gagcttgacg atcgcctgcg
      121 gcgtgcgagc gacctgaaaa agcttcgccg tcgccagatt gacgctcttg tcggtcgctt
      181 gagcggatag caccaccgcg atcagcagtt cgaacggcgt gcgataggtg agttcgcttc
      241 gcggatgcgg attctgctgg cgcaatcgct cgtagatctc gcgccgcttc gccggattca
      301 ccggctttcc cgagcccggc gggcgcgggc tcgggcgagc gctgccgcga cggcctgccg
      361 gcgcgcgtcg ggcgtcgcat tctgcacgcg aggcctggtc ttgacgtccg ggcgcggtgt
      421 gcgcttggcg gtttcttgcg cgagccgaag cttgcgcgat tcaaaccggg tgcgcgccgc
      481 cttcgcgtcg tcttcggtcc acgcgcgccc cgcgctcgtc atgacgatgc aatcgaccgg
      541 acacggcggc aggcaaagtc cgcagccggt gcagtgatcg gccagaacgg tatgcatccg
      601 cttggcagcg ccgacgatcg cgtcgaccgg acaggcctgg atgcacagcg tgcagccgat
      661 acagacggtc tcgtcgatgc tcgccagtgt cagcggctcg gcgacgccga aggcggggtc
      721 gggtgatggc gccggcacat cgagcagcgc ggcgagtgcc gcgatcgttg ccgtgccgcc
      781 cggtgcgcag cggttgatcg gcgcggtgcc ggcggcgatc gcctcggcat acggacgaca
      841 cccggggtgc gcgcagcggc cacactgcgt ctggggcagc aggtcgtcga tgcggtcggc
      901 cagcgcggcg gtattgcgca tgccgacatt ctactggcgc taagcgttgc ccgcgatgcg
      961 ggcttggtcg acaatgaggc acgcgagcca tcatgcctgc ccaccaccat tcccctgcaa
     1021 ggccgcgcgg cgacacggcg acaagcgcag cggtgcgcga acgccgcgcg ctgcgtctgg
     1081 ccgtcgcgtt gacggtcggc tttgggctga tcgaagcggc cgggggctgg ctcgcaggct
     1141 ccctggcgct gctcgcagac gccggccaca tggtcaccga tgccgcggcg ctgggcatcg
     1201 cactgctggc ccagcacgtg gcgcgactgc cgccctcgcg tcgcgcgagc tatggctacg
     1261 cccgggccga ggtgatcgcc gcgttcatca atgcgctgct gatgcttgcc gtggtggtcg
     1321 ggatatcggt cgaggccgtg cgccgcctgc tggcaccttc acccgttgcc ggcggcatgg
     1381 tgatggcgat cgccgccatc ggcctgctga tcaacatgtt cgcggcctgg agcctgtcgc
     1441 acgggcacag cctgaacagc cgggccgcat tgctgcacgt cagcggcgat atgctgggtt
     1501 cggtggcggc aatcgtcgcc ggcgccgtcg tcgcctcgac cggatggatg ccgatcgatc
     1561 cgatcctgtc gctgctggtg gctgggctgg tgctgcgctc gacctggctc ttgctcaagg
     1621 cttcgaccca cgtgctgatg gagggcgtgc ccgcccatct tccgtatgag gaaatcgggc
     1681 gcgcactgac gcgggtccag ggcgtggaag ccgtgcacga tctgcatgtc tggcagatga
     1741 ccagcgaacg ggccgcgttg tctgcgcatc tgctgatccg cgacccgcag gcatggccgc
     1801 agatcctggc ggcggcgcag cgcctgctgg ccgaccggta ccagatcgat cacgtcacgc
     1861 tgcaaccggc ctggcacgcg ccgccggcgg acaagcgcgt gattccgctg aagccgatcg
     1921 ccggcgacga caagccccac ctgcactaaa cgcgcgaggc tgcccggctg ctacagccag
     1981 cggatcctct tgaaccagag gtagagtccg acatcgatcg cggccatcac cgcgagcgat
     2041 atctgatagc catactgaag atcgtactcg ggcatgttct tgaagttcat gccgtagata
     2101 ccggcgatca gcgtgggcac cgcgatgatg gcggcccagg ccgcgagttt tttggttacc
     2161 tcggtttcgt tcaggttgat catgcccaag ttgacctgga tcgccgtcgc ctgcatgtcg
     2221 cggatgccct cgatcgacgc atgcaggcgc tgcagatggt cgaacacgtc gcggaaatac
     2281 tcgcccatgc cggcgcagat gcgcggcacg cggccgccgt agagcttgcc gacggcctcc
     2341 ataagcgggt cgacggcgtg ctttagcgtc atcagcttgc gcttgaggtc gtacagcgcc
     2401 tcgatgttgg agcgcgccgc gttgcggacg aaaatctgct cctcgactgc ttccagctcg
     2461 gtctccagcg tttcgaggac cggaaagtag cgatcgacca ccttgtccat cagcgcatag
     2521 aggacatacg cagccccgtg cttgagcagg tcgggttcgt gttcgctgcg tgcgcggacg
     2581 tcggcgaacc cgcgctcggt atcgcggcga atcgacaaca cgtaattggg cccgacgaat
     2641 atgtcgattt cgccgacgtg gagctcgttg tcgcgcattt cgacggcgtg cagaacggcg
     2701 aacagcgagt ccccgtactc ctcgatcttg ggccgctgat gtccgtgccg cgcgtcctcg
     2761 accgccagcc cgggcaggtc gaactcctcg cgcatttcct cgagctcgtc cggcggcgga
     2821 tccttcagcg ccacccagac gaacgtatcg ggacgcttca agtaatcgct gatcgcttcc
     2881 ttcggaatgt cggcgagctt cttgccttcc tgataggcga cgcagttgac cagcatggat
     2941 cgttgccccg atggcgaatg atcgatgata gcggccccgg cccgcgattg ccaccttgcg
     3001 ccgacccgtc ggacgccttg tcagcccgga tgcttcgccg tccggcgaaa gtggataccc
     3061 tcgccggcag cgggtaccat ggctgccttt ccgccgctcg cgaaacccgc atgatcatcg
     3121 agtcgctgct cgataccgac ctgtacaagt tcacgatgat gcaggtcgtg ctgcaccatt
     3181 tccccgccgc gcaggtcgaa taccgcttca aatgccggac acccgacgtc gacctgactc
     3241 cgtacctgga tcagatcgaa cgggagatca tcgggctgtg cgcccttcgc ttcacgcacg
     3301 aggagctcga atacctgcgg cgctggcgct tcctcaagag cgacttcgtc gatctgctcg
     3361 gtctgttcca gctcgaccgg cgcttcatca gcctgacacc cgcggcggcg ccgggcgaaa
     3421 tcgacatcac gatcaagggg ccgtggctgc acacgatcct gttcgaggta ccggtgctcg
     3481 cgatcgtaag cgaagtgtat ttccgcgaga cgatagcccg gcccgacctc gccgaggggc
     3541 gccgccgcct cgacgccaag atcgcgcaga tcaatgcggt tcccgacccc gcgttccgga
     3601 tcgccgacta cggcacgagg cgcaggttct cgcgcgagtg gcaggacgaa gtcgtgcgca
     3661 cgctgaagga ggggatcggc cccaagttcg tcggtaccag caacgtcaag ctcgcgttcg
     3721 agcacaacct caccccgctg ggcacgatgg cgcacgaata catgcaggct tgccaggcag
     3781 tgggaccgcg gctccgcgat tcgcaggtgt tcgcgttcaa cacttgggcg cgcgaatacc
     3841 ggggcgacct gggaatcgcg ctgtcggata tctgcggcat ggacgcgttc ctgcgcgatt
     3901 tcgacctttt cttctgcaag ctgttcgacg gcgtgcgaca cgactcgggc gatccgttcg
     3961 aatggggcga gaagatgatc gcgcattacc agaagatgcg catcgatcca cgctcgaagg
     4021 tgatggtctt ttccgattcg ctcaacgcgc cgctggcgat ccggctgttc gaatacttca
     4081 agggtcgcag tcagacggcg ttcggcgtcg gcaccaacct gaccaacgac ctcggctacg
     4141 acgcactgca gatcgtgatc aagatgacgc gctgcaatgg ccagccggtc gccaagatca
     4201 gcgacgagcc atcgaaggcg atggattacg atccgtcgta cgtcgcctat ctgcgcgagg
     4261 tgttcgaagt gccggaagcc gtggcaaccg ctggcggcga tgggctcgac gccgattagg
     4321 gttcggcgag accggcccgg tatgcggcca gcacgtccgc gccggcgcaa acgaacacga
     4381 ccctctgcag cgcatcgagc cggtcgaagg ctcgcaccgt cgcgactgcg atcggcactg
     4441 cctttgccag cggataccca tacgcgccgc agctgatcgc cgggaatgcg atcgacgcga
     4501 gtccatggcc gacggcaagc tccagcgaat gccggtagca cgaagccagc aattgcggct
     4561 cgccggcatt gccgccatgc cacaccgggc caaccgtgtg gatgacgtgg cgcgcgggca
     4621 ggcgatagcc gcgcgtaatc ttggcgtcgc cggtggcgca gccgcgcaag gcccggcatt
     4681 cggcgagcaa ttcgggtccg gccgcgcgat gaatcgcgcc atcgacgccg ccgccgccca
     4741 agaggctcgt gttggccgca ttgacgatcg cgtcgacggt gagcgtcgtg atatcggcgc
     4801 gaacagcgat gatttccgga tgcatggcgt cattttcgca ttccgccggg ccgacagcgg
     4861 gtcgttcgcg gcgacggcgg ccggcctagg cctgctgtcg cgccacg
//
LOCUS       DATANS010000482         4883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_780177, whole genome shotgun sequence.
ACCESSION   DATANS010000482 DATANS010000000
VERSION     DATANS010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780177"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VHT22_07125"
     CDS             complement(<1..433)
                     /locus_tag="VHT22_07125"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX3137363.1"
                     /translation="MITRPARQAAQLAQQIAAVGGTPLIFPTIAILPPDDTRALDDVH
                     RRLAQFDYAIFVSANAVEFGIGDPAAWPAALPAFGPGPGTAAALAGAGIGDVRQPRTT
                     MDSEGLLELDEFTAPSGKRVVIFRGGTGRDLLADTLTERGAH"
     gene            complement(460..1407)
                     /gene="hemC"
                     /locus_tag="VHT22_07130"
     CDS             complement(460..1407)
                     /gene="hemC"
                     /locus_tag="VHT22_07130"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX3137364.1"
                     /translation="MSAATALPARLVIATRRSALAMWQAEHVRSRLAVLYPYCAIELL
                     GLTTQGDRVTDQPLADIGGKGLFIKELEQAMHEDRADLAVHSLKDVPMDMPQGFTLAA
                     IMTREDPRDALVSRRCRSLSELSPASVVGTSSLRREAQLRERYPQLAVEPLRGNVNTR
                     LDKLDAGQYAAIILAAAGLRRLGLAQRIAALLDPAESLPAIGQGALALECRTDRADVL
                     AALAPLSDAATTRATTAERAFGRVLAGNCRTPLAAYAMDRGPQLWLRGLVASRDGSQV
                     LRGERVAATSTVEEAAALGEALGAEFLQRGAAAMLAENV"
     gene            complement(1523..2230)
                     /locus_tag="VHT22_07135"
     CDS             complement(1523..2230)
                     /locus_tag="VHT22_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007252734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX3137365.1"
                     /translation="MRIRDSVSPEENDAALWMARREDRIIRWGFCFGTLAMPHPTHLR
                     ICAAIEAHGWAAEAAFLPEQAIAALRAEARRRDAAGEFHAAGVGRGPARVERSDIRGD
                     RILWLDEHARSSAEVPLWQALAALQAALNRALFLGLDSFEGHYALYPSGAFYRRHRDR
                     FRDDDTRVISCALYLNEDWSADDGGALRLHMPEGLRDVVPEGGVLACFLADRFEHEVL
                     PAARERLAVSGWFRRRA"
     gene            2223..3197
                     /gene="denD"
                     /locus_tag="VHT22_07140"
     CDS             2223..3197
                     /gene="denD"
                     /locus_tag="VHT22_07140"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HEX3137366.1"
                     /translation="MRIVITGGAGFLGTRLARKLLERGSLVDAAGQCRPLRQLVLLDV
                     AAAAVNDPRVTAVAGDLADPAVIERVVTIDTDSIFHLAAVVSGQAEAEFDTGMRVNLD
                     ATRALLERCRKLNKPPKFVFTSSLAVFGGKLPDPVPDDAPLTPQTSYGAQKAIGELLV
                     YDMTRKGYIDGRSLRLPTVTVRPGKPNKAASSFASGIIREPLSDVDAVCPVAPETELW
                     VTSPRQVIDNLIVGHDVAASKFGQTRSVNVPGLRISVGAMVDALRRVAGDAVAARVKW
                     QIDPVIDRIVQTWPPNFAPRLGPALGMGADTDFDEVVRAYIADGMLAG"
     gene            complement(3219..3758)
                     /locus_tag="VHT22_07145"
     CDS             complement(3219..3758)
                     /locus_tag="VHT22_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX3137367.1"
                     /translation="MYLAILLLLKGMKAVANLVRPFTALLPDWIPAEDLLSLLLVLAI
                     CAVIGATVGTRIGRGARDWLERSVFERIPGYGVIRSLTRQMAGKSTESTWRAALIEIE
                     DALVPGFIIEEFEDGRYTVFVPSIPTPFAGAVYVLDRQRVHPVDVPFTDAIRVISRWG
                     SGAKNLVAAMERRESVAPR"
     gene            3948..4871
                     /locus_tag="VHT22_07150"
     CDS             3948..4871
                     /locus_tag="VHT22_07150"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX3137368.1"
                     /translation="MKPDWRGVFPAVTTQFREDLSLDVDATARVIEGLIRDGVSGLIV
                     CGTVGENCSLSKDEKIEVMETAKSVARGRVPVISGIAEFTSAFAIDMAREAARIGLDG
                     VMVMPALVYSSKPHETAAHFRSVAKATDLPVMVYNNPPIYKNDVTPEILASLADCDTI
                     VCFKESSGDTRRFVDLKNMVGDRFVLFAGLDDVVVESILVGAVGWVSGLSNAFPREGE
                     TLFRLASTGRYDEAMSLYRWFMPLLHLDARPDLVQCIKLCEHLVGRGTHRTRPPRLPL
                     SPKETAEIEAMMKHALATRPTLPDVGLRRAA"
BASE COUNT          829 a         1652 c         1621 g          781 t
ORIGIN      
        1 cgtgcgcgcc gcgctcggtc agcgtgtcgg ccaacaagtc gcgcccggtg ccgccgcgaa
       61 aaatcacgac gcgcttgccg gaaggcgcgg tgaactcatc cagctcgagc aggccttcgc
      121 tgtccatcgt cgtgcgcggc tgacgcacgt cgccgatccc ggccccggcg agcgccgccg
      181 ccgtgcccgg cccgggcccg aacgcgggca gtgccgccgg ccaggcggcc ggatcgccga
      241 taccgaattc cacggcattt gcgctgacga aaatcgcata atcgaattgc gcgagccgcc
      301 ggtgcacgtc gtcgagtgct cgagtgtcgt cgggcggaag aatcgctatc gtcggaaaga
      361 tcagaggcgt gccgccgacg gcggcgattt gctgcgccag ctgggcggcc tgccgcgcgg
      421 ggcgcgtgat cacgatgccc gcgccgcgaa gcggcccgct catacatttt ctgccagcat
      481 cgcggcggcg ccgcgctgca ggaattcggc gccgagcgcc tcaccgagcg cagctgcctc
      541 ctcgacggtg ctagtcgctg cgacgcgctc gccgcgcagc acctggctgc cgtcgcggct
      601 agcgaccagc ccgcgcagcc acaattgcgg gccgcggtcc atcgcatacg ctgcgagcgg
      661 cgtgcggcag ttgcctgcaa gcacgcgccc gaatgcccgt tctgcggtgg tcgctctcgt
      721 cgtcgctgca tccgaaagcg gcgccagcgc ggcgagcacg tcggcgcggt ccgtgcggca
      781 ctcgagcgcg agcgcgccct gcccgatcgc gggaagactc tctgccggat cgagcagcgc
      841 cgcgattctt tgcgcgaggc ccaggcgccg caagcccgcg gcggcaagaa tgatcgccgc
      901 atactgtccg gcatcgagct tgtcgagccg ggtgttgacg ttgccgcgca gtggctcgac
      961 ggcaagctgc ggatagcgct cgcgcagctg cgcttcgcgt cgaaggctcg atgtgccgac
     1021 gacgctcgcc ggcgacagct cggaaagcga ccggcatcgt ctcgatacca gggcgtcgcg
     1081 cggatcctcc cgcgtcatga tcgccgccag ggtaaagccc tgcggcatgt ccatcggcac
     1141 atccttgagc gaatgcacgg ccagatcggc gcggtcctcg tgcatcgcct gctcgagctc
     1201 cttgatgaac agccccttgc cgccgatgtc ggcgagtggc tgatcggtca ccctgtcccc
     1261 ttgtgtcgtc aggccgagca actcgattgc gcagtaggga tagagcaccg cgagacgcga
     1321 gcgcacgtgc tcggcctgcc acatcgcgag cgccgaccgg cgcgtcgcga tcaccaatcg
     1381 tgccggcagt gcggtcgcgg cggacatctt caccaaggct ggggatggaa gtacggcggg
     1441 aggtcggcca tcgaagcggc aaggcttcga tcgaccgtat cgattctagc atgggcgaga
     1501 gcgtcgattt cgcgcgccac ggttacgccc ggcggcgaaa ccagccgctc accgccaggc
     1561 gctcgcgcgc ggccggcagg acctcatgct cgaagcggtc ggcgagaaag caggccaaca
     1621 cgccgccttc cggcacgaca tcgcgcagcc cttctggcat gtgcaggcgc agggcgccgc
     1681 cgtcgtcggc cgaccaatcc tcgttcagat agagcgcgca cgaaatgacg cgcgtgtcgt
     1741 cgtcgcgaaa tctgtcgcga tgccggcgat agaacgcgcc ggacggatac agcgcgtagt
     1801 ggccctcgaa cgaatcgagg ccgaggaaca acgcccggtt gagcgcggcc tgcagtgccg
     1861 ccagcgcctg ccagagcggc acttcggccg aggagcgcgc atgctcgtcg agccaaagga
     1921 tgcgatcgcc gcgaatgtcg ctgcgctcga cccgcgctgg gccgcggccc acgcccgcgg
     1981 catgaaattc gcccgcggcg tcgcgccggc gcgcctcggc gcgaagcgcg gcgatcgcct
     2041 gctccggcag gaacgcggct tccgcggccc agccgtgcgc ttcgatcgct gcgcaaatcc
     2101 taaggtgggt cgggtgcggc atcgcgaggg taccgaagca aaaaccccat cttataatgc
     2161 ggtcttcgcg ccgcgccatc caaagtgcgg cgtcgttttc ctctggagac acggagtccc
     2221 tgatgcgcat cgtgatcacc ggcggcgccg gtttcctcgg cacccgcctc gcccgcaagc
     2281 tgctcgagcg cggcagtctc gtcgacgcgg cgggccagtg tcgcccgctg cggcaactgg
     2341 tgctgctcga cgtcgcagcg gcggcggtaa acgacccgcg ggtcacggcg gttgccggcg
     2401 acctggccga tccggcggtc atcgagcgcg tcgtgacgat cgacaccgat tcgatatttc
     2461 atctcgccgc cgttgtcagc ggacaggcgg aagccgagtt cgataccggt atgcgcgtca
     2521 acctggacgc gacgcgggcg ctgctcgaac gctgccgcaa gctgaacaag ccacccaagt
     2581 tcgtgttcac cagctcgctc gcggtcttcg gcggaaaatt gcccgacccg gtgcccgacg
     2641 atgcgccgct gacgccgcaa acctcgtatg gcgcgcagaa ggcgatcgga gagctgctgg
     2701 tctatgacat gacgcgcaag gggtacatcg acggccgcag cctccgcctg cccacggtga
     2761 cagtgcggcc cggcaagccg aacaaggccg cgtcctcgtt cgcgagcggc atcattcgcg
     2821 agccactgtc ggacgtcgat gccgtatgtc cggtcgcgcc ggagaccgag ctgtgggtaa
     2881 cctcgccgcg ccaggtcatc gacaacttaa tcgtcggcca cgatgtcgcg gcatcaaagt
     2941 tcgggcagac gcgctcggtc aatgtccccg ggcttcgcat ttcggtcggt gcgatggtcg
     3001 acgcattgcg tcgcgtcgcc ggcgatgccg tggcggcccg ggtcaaatgg cagatcgatc
     3061 cggtaatcga caggatcgtg caaacctggc cgccgaactt cgcgccgcgg ctcggccctg
     3121 cgctcggcat gggagccgac actgacttcg acgaagtcgt gcgcgcttac atcgccgacg
     3181 gcatgctcgc cggctagcgt cgttcagaaa gggctgcgtc agcgcggcgc gacggactcg
     3241 cggcgctcca tcgccgcaac caggttcttc gcacccgatc cccagcgcga gatgaccctg
     3301 atcgcatcgg tgaacggcac atccaccggg tgcacccgct gccgatcgag aacgtagacc
     3361 gcacccgcga acggcgtcgg gatcgacggg acgaagacgg tgtagcgccc gtcctcgaac
     3421 tcttcgatga tgaaccctgg gacaagggca tcctcgatct cgatcagcgc tgccctccag
     3481 gtgctctcgg tgcttttccc agccatctgg cgcgtgaggc tgcggatgac cccatagcca
     3541 gggattcgct cgaagaccga ccgttcgagc cagtcccgag cccctcgtcc gatgcgagta
     3601 ccgacggtgg ctccgatgac ggcgcagatc gcgagaacca gcagcagcga caggaggtct
     3661 tcggcgggaa tccaatcggg gagaagcgcc gtgaacggcc gcaccagatt ggcgaccgcc
     3721 ttcatcccct tgagcagcag caggatcgcc agatacaccg gaacgacaat aagcaaacct
     3781 cgaatcagcg ctttcgagac ggctacgaat gcctgcttca tgagtgtctt ctccgttgtg
     3841 gaatcgactg gcgtactcgg ttcgttgtgg agtcgccaag agttccctat gatgctcgct
     3901 attcgggaag tcatggaagt gttggccaaa aagggaagga gggatgattg aagcctgatt
     3961 ggcgcggcgt atttcccgcg gtgacgaccc agtttcgcga ggacctctcg ctcgacgtcg
     4021 acgccaccgc gcgcgtcatc gagggcctga tccgcgacgg cgtttcgggc ctgatcgtct
     4081 gcggcacggt tggcgagaac tgctcgctgt ccaaggacga gaagatagag gtcatggaga
     4141 ctgcgaagtc ggttgcgcgc gggcgcgtcc cggtcatcag cggcatcgcc gaattcacgt
     4201 cggccttcgc gatcgacatg gcgagagagg cggcgcgcat cggcctcgac ggcgtgatgg
     4261 tcatgccggc actggtctac tcgtcgaagc cgcacgagac cgccgcgcac ttccgcagcg
     4321 tcgccaaggc gaccgatctg ccggtgatgg tctataacaa cccaccgatc tacaagaacg
     4381 acgtcacgcc cgagatcctg gcctcgctgg ccgattgcga tacgatcgtc tgcttcaagg
     4441 aatcgtccgg cgacacccgg cgcttcgtcg atctgaagaa catggtcggc gatcggttcg
     4501 tgctcttcgc cggactcgac gacgtcgtcg tggagagcat cctggtcggc gccgtcggtt
     4561 gggtgtccgg gctgtcgaat gccttcccgc gcgaaggcga gacactgttc cggctggcca
     4621 gcaccggccg ctacgacgaa gcgatgagcc tctaccggtg gttcatgccg ttgctccatc
     4681 tcgatgcccg ccccgacctc gtccagtgca tcaagctttg cgagcacctc gtcgggcgcg
     4741 gcacgcatcg cacgcgtccg ccgcggctgc cgctcagtcc gaaggaaacc gcggaaatcg
     4801 aagcgatgat gaaacacgcg ctggcgacgc ggcccacgct gccggacgtc ggcctcagac
     4861 gagcggcctg acgagcgagt ccg
//
LOCUS       DATANS010000483         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_403450, whole genome shotgun sequence.
ACCESSION   DATANS010000483 DATANS010000000
VERSION     DATANS010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403450"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="VHT22_07155"
     CDS             complement(<1..151)
                     /locus_tag="VHT22_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX3137369.1"
                     /translation="MTDRNIPVTEDELHAYVDNELPAERRSDVEAWLASHPDDAARVQ
                     SWRTMA"
     gene            complement(210..698)
                     /locus_tag="VHT22_07160"
     CDS             complement(210..698)
                     /locus_tag="VHT22_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX3137370.1"
                     /translation="MTAFRQSVEAMIPALRRYARALARDADIADDLVQDTLVRALRSE
                     RLFLGGDVRSWLYTILTNLNKNRRRSLARRPQFMPLLDNNPDASGTEAEGRDIARALA
                     TLVEEQRSVLLLVMLEGLSYREVADIQGVPIGTVMSRLARARAHVKASLEGERTALRR
                     VK"
     gene            1006..>1503
                     /locus_tag="VHT22_07165"
     CDS             1006..>1503
                     /locus_tag="VHT22_07165"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX3137371.1"
                     /translation="MPAPVIYYIRHGETSWNAEGRLQGTQDVALNDLGRKQAAHAGNV
                     LADLLTRDGRDKATLPFVASPLQRARATMELVRGALELPPEKYALDDRLREIGYGVWE
                     GLTLAESEAADPEIYARRLADKWTVGPAGGETYADVQVRVRAWYDQLRTDTVAVAHGG
                     TCRALM"
BASE COUNT          278 a          495 c          477 g          253 t
ORIGIN      
        1 ccgccatcgt gcgccacgac tgcacccgcg cggcatcgtc gggatgcgag gcgagccacg
       61 cctcgacgtc gctgcggcgt tcggccggca gctcgttgtc gacgtaagca tgcagctcgt
      121 cttcggtgac gggaatgttg cgatcggtca ttgtcgtcgt ctctgcctat tcctggtctg
      181 tctgtgcatc acgcatgcca ctcgcacctt catttcaccc gccgcagcgc cgtacgctcg
      241 ccctcgagcg aagctttgac atgcgcgcgt gcgcgggcaa gccgggacat tacggtgccg
      301 atcggcacgc cctggatgtc ggcgacctcg cggtagctca gtccttccag catcaccagc
      361 agcagcaccg agcgctgttc ttccaccagc gtcgccagcg cgcgggcgat atcgcgaccc
      421 tcggcctcgg tgccgctggc gtcagggttg ttgtcgagca gcggcatgaa ctgcggacgc
      481 cgcgccagcg accgccgccg gttcttgttc aaattggtca ggatcgtata gagccagctc
      541 ctgacgtcgc cgccgagaaa cagccgctcc gaacgcaacg cacgcaccag cgtatcctgc
      601 accagatcgt cggcgatgtc ggcatcgcgc gcgagcgcac gtgcatagcg acggagcgcc
      661 ggaatcatgg cttcgacact ttgacgaaac gcggtcatcg gcaccagatt gcaaagggtt
      721 gtccggcgcg aacgccttac ctaacataac acccaatcgg cgcgtctatt ccagccattg
      781 aggcgcatcg aaaacagcgt taatccggaa gccgattcgg gctcgaccgc gctggagtgc
      841 tggtgtacct ccaagctcac agggacgcgc gattggaaag caaggtgtcg caaaaatcag
      901 gtctgcaggg caaacgcagg gtgatcctcg gcgtcgccaa agcgctccga cgcgcccgac
      961 aattcgctct ccaatttgcc gagcaggaaa tagcctgaca gttcgatgcc agcgcccgtg
     1021 atctactaca ttcgccatgg cgagacgtcg tggaacgcgg aaggcaggct gcaaggcacg
     1081 caggacgttg cgctgaacga tcttggccgc aagcaggcgg cccatgccgg caatgtcctc
     1141 gccgatctcc tcacgcgcga tggccgcgac aaggcgacgc tgccatttgt cgcaagcccg
     1201 ctgcagcggg cgcgggcgac gatggaattg gtgcgcggcg cgctggaact gccgccggaa
     1261 aaatatgcgc tcgacgatcg cctgcgcgag atcggctacg gcgtctggga agggctgacg
     1321 ctggccgaga gcgaggccgc cgatcccgaa atctatgcca ggcgcctcgc cgacaaatgg
     1381 acggtgggcc ccgcgggcgg ggagacctat gccgatgtgc aggtccgcgt ccgcgcctgg
     1441 tacgatcaac tccggaccga caccgtcgca gttgcccatg gcgggacctg ccgggccctg
     1501 atg
//
LOCUS       DATANS010000484         3797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_780441, whole genome shotgun sequence.
ACCESSION   DATANS010000484 DATANS010000000
VERSION     DATANS010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780441"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1136
                     /locus_tag="VHT22_07170"
     CDS             156..1136
                     /locus_tag="VHT22_07170"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX3137372.1"
                     /translation="MRTTALFFWRTCCAIVLLTCLNGAHAQPYPSKSIRLIVPYAAGG
                     STDALARAVGQKLGDAFGHQVIVDNRTGANGNIGTDMVAKASADGYTLLMAFDATMVI
                     NPAVYAKLPFDPVKDFAPITKVAALPLILVAHPSFPPNNTKELIPYAKSKPGLNYSSS
                     GHASTPHLAILLFEQRTGTQFTHIAYKGGGQAVIDVLAGQIPLLATAIPTVQAHIRAG
                     KLKGIALTSAKRHASLPDVGTFAEAGIAGFDISAWYGLLAPAGTPRVVVMRVHDETVK
                     ILATPEMKDRFQNTLGGDAVGNTPEQFAANIRDDIERWGKIVKQTGIKLE"
     gene            1209..2198
                     /locus_tag="VHT22_07175"
     CDS             1209..2198
                     /locus_tag="VHT22_07175"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX3137373.1"
                     /translation="MNYRAVGCTIVALALSSAAHHVLAQAPKDAAASYPSRPIRLIVP
                     VAPGGSSDGMARILGQKLTDSWNQQVLVDNRPGASEIIGTDLVAKGVPDGQTIVLVSL
                     RFSVNPSLLKLPYDSVKDFEPVSMTAAVGNVLVVNVKSPIMSVKDLIAAAKQKPGELV
                     FASSGLGGAPHLVGEFFALTTGVKLTHVAYKGGGPAVADLLGGNVAMSFASMTSALQF
                     VKAGRLRALAVSSKERSALLPDVPTMAEAGVPNIVVRDWQGVLAPHGTPKPIVDKLSG
                     EIRRILRDPASQERFAVLGLEIIASTPQEFRAAIESEIQRWAKVVKEANIKAE"
     gene            complement(2207..3169)
                     /locus_tag="VHT22_07180"
     CDS             complement(2207..3169)
                     /locus_tag="VHT22_07180"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX3137374.1"
                     /translation="MKSALYFAMLAATAAGAQTAPQYPAKPIRMIVTAAAGSGPDITA
                     RIVGQKLTAALGQPVIIDDRPGAGGSIAAELAAKAPPDGYTLVMASAGSHAVSPALYT
                     KLPWDPVKDFVPITIVALAPNILIVHPSLPVKSVRELIALAKARPGQLSFGSGGSGST
                     AHLSGELFRTMANINIVHIPFKGAPSAALGVIGGQVEIALLNLPPTLPQVKSGRLKGL
                     AVTTAKRTSAIPELPTIAEAGVPGYEASTWYGVMAPAGTPGEITGRLYTAIIADLRTD
                     DTRARIAADGGEVVGSTPEEFAAVLKRDIAKWTRVVKDSGARAD"
     gene            complement(3171..>3797)
                     /locus_tag="VHT22_07185"
     CDS             complement(3171..>3797)
                     /locus_tag="VHT22_07185"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3137375.1"
                     /translation="LITEALTSRAFDLRAVAVVAEDISDRELETMHAAGYRGIRINTA
                     SATPGLKLAQAPKLAARIKPLGWHLQLFVNLRDMPQMEDELAQLKIDIVIDHFARIAT
                     ADGPAAPPYQALLRLLRRSNVWAKLMGPYFISDAAPHYPDIAPFARGMIAAAPDRIVW
                     GSDWPHPSAREKMPDDGDLADLINEWAPDMAQRRKILVDNPRRLYGFE"
BASE COUNT          708 a         1231 c         1181 g          677 t
ORIGIN      
        1 cgtgcgcgca aggcgcctgc tttggatgtc ataggtggtt cctgtcggta cgcgcggccg
       61 cgtgtgccat actatcggca gccaatggct ttctgcgcgt gaaaccggat gatagaccaa
      121 tcggcagccg cgcatcgccg cgacgaggag aaaacatgcg cactaccgct ctttttttct
      181 ggcgcacttg ctgcgccatc gttctgctga cgtgcctcaa tggcgcccac gcgcaaccct
      241 atccgagcaa gtccatccgg ctgatcgttc cctatgcagc cggcggatcg accgatgcgc
      301 tggcgcgcgc cgtgggccag aaactcggcg atgcattcgg ccaccaggtc attgtcgaca
      361 atcgcacggg cgccaacggc aacatcggca ccgacatggt cgcgaaagcc agcgccgacg
      421 gttacacgct cttgatggcg ttcgacgcga ccatggtcat caatccggcg gtgtacgcga
      481 aactgccgtt cgacccggtc aaggatttcg caccgatcac caaagtcgcg gcgctgccgc
      541 tgatcctcgt cgcgcatccg tcgtttccgc cgaacaacac gaaagaattg attccgtacg
      601 cgaagagcaa gcccgggctg aattattcgt cgtcgggcca cgcgagcacg ccgcatttag
      661 cgatcctgct gttcgagcag cgcaccggca ctcagttcac gcatatcgcg tacaaaggcg
      721 gcggccaggc ggttatcgac gtgcttgcgg gccaaattcc gctgctcgcg accgcgattc
      781 caaccgtcca ggcgcacatc agggccggca agctgaaagg cattgcgctg accagcgcaa
      841 agcgtcatgc ctcgctgccc gacgtcggga cgttcgcaga ggcgggcatc gccggcttcg
      901 acatttccgc atggtatgga ttgctcgccc ctgccggcac gccccgcgtg gtcgtgatgc
      961 gcgtgcacga cgaaaccgtg aagatcctcg cgacgcctga aatgaaagac cgcttccaga
     1021 atacgctcgg cggggacgcg gtcggcaaca cgcccgagca gtttgccgcg aacatccgcg
     1081 acgatatcga gcgctgggga aaaatcgtga aacagacggg gatcaagctc gaatagcggg
     1141 catcgtcttt gctaggtgag gaccgtgaaa acgcgttttt aaaaaatccg cacgaagaca
     1201 gggcaaacat gaactatcgc gccgttggtt gcaccattgt tgcgctcgcg ctgtcgagcg
     1261 cagcccatca tgtactcgcg caggcgccga aagatgcggc cgcctcctat cccagccgcc
     1321 cgatccgttt gatcgtgcct gtcgcaccgg gcggcagcag cgacggcatg gcgcgcatcc
     1381 tcggccagaa actgaccgac agctggaacc agcaggtgct cgtcgacaac cggcctggcg
     1441 cgtccgaaat catcggcacc gatctggtcg cgaaaggcgt gcccgacggc cagaccattg
     1501 tgctggtgtc gctgcgcttc tcggtaaatc cgagcctgtt gaagttgccg tacgactcgg
     1561 tgaaagactt cgaaccggtc agcatgaccg cggcggtcgg caacgtgcta gtcgtcaacg
     1621 tgaaatcgcc gatcatgtcg gtgaaagacc tgatcgccgc cgcgaagcaa aaacccggcg
     1681 agctcgtgtt tgcgtcgtcg ggcctgggcg gcgcgccgca cctggtaggt gaattctttg
     1741 cgctgacgac aggcgtcaag ctcactcacg tcgcttacaa aggcggcggc cctgcagtcg
     1801 cggacctgct cggcggcaac gtcgccatga gtttcgcgtc gatgacttcg gcgctgcagt
     1861 tcgtcaaggc cggccgcttg cgtgcgcttg ccgtatcgtc gaaggaacgg tcggcgctat
     1921 tgcccgacgt gccgacgatg gcggaagccg gcgtgcccaa catcgtcgtg cgcgactggc
     1981 agggcgtact cgcgccgcac ggcacgccca aaccgatcgt cgataaactt tcaggagaga
     2041 ttcgccgcat cctcagggac cctgcgagtc aggagcgctt cgctgtcctc gggctcgaaa
     2101 tcattgcgag cactccgcag gaattccgcg cggctatcga atccgaaatt cagcgctggg
     2161 cgaaagtcgt caaggaagcg aacatcaagg cggagtgacg gcgggttcaa tcggcacgcg
     2221 cgccggaatc cttaacgacg cgcgtccact tggcgatatc gcgctttaac accgccgcaa
     2281 actcctccgg cgtgctgccg acgacttccc cgccgtcggc cgcaatgcgc gcacgcgtat
     2341 cgtcggtgcg caggtccgcg atgatggccg tatagagtcg tccggtgatt tcacccggcg
     2401 tgcccgccgg agccatcacg ccgtaccacg tcgaggcttc atagccgggc acacccgctt
     2461 cggcgatggt tggcaattcc ggaatcgcgc tcgtgcgctt cgccgtcgtc actgcgagcc
     2521 ctttcaaacg accgctcttc acctgcggca gcgtcggcgg cagattgagc agcgctatct
     2581 cgacctgccc gccgatcacg cccagcgcgg cggaaggcgc acccttgaac ggaatgtgca
     2641 cgatgtttat attggccatc gtgcgaaaca attcgcccga cagatgcgcg gtgctgccgc
     2701 tgccgccgga gccgaatgac aactggcccg gacgcgcttt ggcgagcgcg atcaattcac
     2761 gcaccgactt gaccggcagc gacgggtgca cgatcaggat attcggcgcg agcgcgacga
     2821 tggtgatcgg cacgaagtcc ttcaccggat cccacggcaa cttcgtgtac agcgcggggc
     2881 tcacggcatg cgatccggcg ctcgccatca cgagcgtata accgtccggc ggggccttgg
     2941 cagcgagttc cgccgcgatc gaaccgcccg cgcccggacg gtcgtcgatg atgaccggtt
     3001 gacccaaagc ggcggtcagt ttctggccga cgatgcgcgc cgtgatgtcg gggccgctgc
     3061 cagcggcggc ggtcactatc atgcgaatcg gcttggcggg atattgcggc gctgtttgcg
     3121 cgcctgccgc ggtcgcggcg agcatcgcga aatacagcgc cgacttcatg tcactcgaaa
     3181 ccgtacagcc ggcgcggatt gtcgacgagt attttccgtc gctgcgccat gtccggcgcc
     3241 cactcgttga tgagatctgc cagatcgccg tcgtcgggca ttttctcgcg cgcgctcggg
     3301 tgcggccagt cggaacccca cacgatgcgg tcgggcgcag ccgcaatcat gccgcgcgca
     3361 aacggcgcga tatccgggta atgcggcgcc gcgtccgaaa tgaaataagg gcccatcagc
     3421 ttcgcccaca cgttgctacg gcgcaaaagc cgcagcagcg cctggtacgg cggcgcggct
     3481 ggcccatcgg ccgtcgcaat gcgcgcgaaa tggtcaatca cgatgtcgat cttcagctgt
     3541 gcgagctcgt cttccatctg cggcatgtcg cgcaggttca caaataactg cagatgccag
     3601 cccagcggct tgatgcgcgc ggcaagtttt ggcgcctgcg cgagcttgag tcccggcgtc
     3661 gccgacgcgg tgttgatgcg gatcccgcga tagcccgctg cgtgcatggt ttcgagctcg
     3721 cgatcgctga tatcctccgc caccactgcg accgcgcgca aatcgaacgc acgcgacgtc
     3781 aacgcttcgg tgatgag
//
LOCUS       DATANS010000485         1749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_360888, whole genome shotgun sequence.
ACCESSION   DATANS010000485 DATANS010000000
VERSION     DATANS010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360888"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VHT22_07190"
     CDS             complement(<1..407)
                     /locus_tag="VHT22_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137376.1"
                     /translation="MRAFIAVIALMMAAAFAEAASLGTAIITQDRVALRASPRDSAKQ
                     QAQLWQGEMVEIRSERLDYLQVYDYGRERGGFVRANQVRRLTLTAEEAPAPARRRRRP
                     RRRSAKARRPRNERRRRAKCGVGPEGQAPLASGG"
     gene            complement(410..1657)
                     /locus_tag="VHT22_07195"
     CDS             complement(410..1657)
                     /locus_tag="VHT22_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX3137377.1"
                     /translation="MLQRLKVMVQSIRERFGRPAAEFGSRAADATSAGMTRAASGIAG
                     AKVVLVVTGVTVIAAYALYQHPPLENIGRGEVGVRINRVTGDISEWREGNVLVLPGLH
                     DIRVFALRDQTYQPEQIRRAEGAAPLQSVEGLSFGVDLSVRYALDAAKLRATSKTLPD
                     NISGEIVEPAVQGVIYKVFARYTVREIFSTKRVEIQQAIEKELTDKLAADGLVLRAVQ
                     MGKVDLPADYRRGMNGLLAEELATEKMRYTLELKDKRVKEIALDGEAEKVRREKAAEA
                     SAREQIIAAKGQEEAMKHVLPFKQRQIEQRQLEAEADKQSRIRVAEGSAQARRVEAGG
                     EADARQKLADAEAYRVERVGKSNAEQMAREGALVTQHPLLIQKTLADKLSDKVQVIIA
                     SPPADGGFIGANLIGTPKRTAEK"
BASE COUNT          249 a          607 c          544 g          349 t
ORIGIN      
        1 cctccgcttg caagtggggc ctggccttca ggcccgaccc cgcatttcgc ccgtcggcgg
       61 cgctcattgc gcggcctcct cgccttcgcg ctgcggcgcc gcggccgccg tctgcgtcgc
      121 gccggcgcgg gcgcctcctc cgccgtcagt gtcaggcgac ggacttgatt cgcgcgcacg
      181 aatccgccgc gctcgcggcc gtaatcgtag acctgcagat agtcgaggcg ctcgctgcga
      241 atctcgacca tttcgccctg ccacagttgc gcctgctgtt tcgccgaatc gcgcggtgaa
      301 gcgcgcagcg ccacgcgatc ctgcgtgatg atcgcggtgc cgagcgacgc cgcctcggca
      361 aaggccgcgg ccatcatcaa agcaattact gcaataaagg cgcgcatgat tatttctccg
      421 cggtacgctt cggcgtgccg atgaggttcg cgccgatgaa accgccgtcc gcgggaggcg
      481 acgcgatgat gacctggacc ttgtcggaga gcttgtcggc gagcgtcttc tggatcagca
      541 gcggatgctg cgtcacgagc gcgccttcgc gcgccatctg ctcggcgttg cttttgccga
      601 cgcgctcgac gcgatacgct tcggcgtccg ccagtttctg gcgcgcatcg gcttcgccgc
      661 cggcttcgac gcgccgcgcc tgcgcgctgc cttccgccac gcggatgcgc gactgcttgt
      721 cggcctcggc ttccagctgg cgctgctcga tctggcgctg cttgaacggc agcacgtgct
      781 tcatcgcttc ctcctggccc ttggcagcga tgatctgctc gcgcgcagag gcctcggctg
      841 ctttctcgcg ccgcactttc tcggcttcgc cgtcgagggc aatctctttt acgcgcttgt
      901 ccttcagctc cagcgtgtag cgcatttttt cggtggccag ctcttccgcg agcaagccgt
      961 tcatgccgcg ccggtagtcc gccggcagat cgaccttgcc catctgcact gcgcgcaaca
     1021 cgagcccgtc cgcggccagc ttgtcggtga gctccttctc gatcgcctgc tggatttcca
     1081 cgcgcttggt cgagaagatt tcgcgcaccg tgtagcgcgc gaataccttg tagatcacgc
     1141 cctgcaccgc cggctcgacg atttcgccgc tgatgttgtc gggcagcgtc ttcgaggtcg
     1201 cgcgcagctt cgcggcatcg agcgcatagc gcaccgacaa atcgacgccg aacgacaggc
     1261 cttcgacgga ttgcaacggc gccgccccct cggcgcggcg gatttgctcg ggctgatagg
     1321 tctgatctcg cagcgcgaaa acgcgtatgt cgtgcaggcc gggcaggacg agcacattgc
     1381 cctcgcgcca ttcgctgatg tcaccggtga cccggttgat gcgcacgccg acctcgccgc
     1441 ggccgatgtt ttcgagcggc gggtgctgat acagcgcata ggcggctatt accgtaacgc
     1501 cggtcacgac aagcacgact ttcgcgcccg caattcccga cgccgctcgc gtcattccgg
     1561 cgctggtcgc atctgccgcc cggctgccga attccgcggc cgggcggcca aagcgctcac
     1621 gaatcgattg caccattact tttaagcgtt gcagcatgac gaactccctt tcaaattaga
     1681 tggttgatac ggatgggtgt atcggtgcgc cggccaggcg ctccgggcca gaaactgggg
     1741 accggcgat
//
LOCUS       DATANS010000486         2744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_610192, whole genome shotgun sequence.
ACCESSION   DATANS010000486 DATANS010000000
VERSION     DATANS010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610192"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1407
                     /locus_tag="VHT22_07200"
     CDS             73..1407
                     /locus_tag="VHT22_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX3137378.1"
                     /translation="MSSANDLFAVVNPATPLAERLRPKSIAEVIGQRHLLGPGKPLAV
                     AFASGKPHSMILWGPPGVGKTTLARLMADAFNAEFIALSAVLSGVKDIRDAIAQAEAT
                     LAQSGRHTILFVDEVHRFNKAQQDAFLPFVEKGLVTFIGATTENPSFEVNSALLSRAT
                     VYVLEALSADELSALLDRALPAAMLGVALNAAARDAVIAYADGDGRRLLNLVEQLAVA
                     AQEAGRREIDSEFVETAITRSLRRFDKGGEAFYDQISALHKSVRGSDPDAALYWFCRM
                     LDGGADPLYVGRRMIRMASEDIGLADPRALQLTLDAVATYERLGTPEGELALAQAVIY
                     LACAAKSNAVYVAYGAARAFIAEDGSRPVPLHLRNAPTRLMKNLGYGKDYRYAHDEED
                     AYAAGESYLPEGMARPGWYRPTDRGLEARIAERLAELRHRDAKARSGGEGNS"
     gene            1455..>2744
                     /gene="serS"
                     /locus_tag="VHT22_07205"
     CDS             1455..>2744
                     /gene="serS"
                     /locus_tag="VHT22_07205"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX3137379.1"
                     /translation="MIDIQLLRNDLDDVVARLKTRGYQLDGPAFVELEAERKHIQMRT
                     QELQARRNTVSKEIGIAKSRGEDASATLREVAGIGDELTSLERNLEAVQQRLRDWLLT
                     MPNVPHASVPVGTSEADNAEVRRWGSPRKFDFAPRDHVDIGAAVGGLDFDVSAKISGA
                     RFYALRGAVARLHRALVQLMLDTHTTEHGYAECYVPYLVNADALIGTGQLPKFEEDLF
                     WAAKGGATDEERWYLIPTAEVPLANFVREQIVAATALPIRLTAHSPCFRSEAGSYGKD
                     TRGMIRVHQFDKVEMVQIVEPDRSYDALEEMVQHAEAILQKLELPYRVVTLCTGDMGF
                     SAAKTYDLEVWLPGQGKYREISSCSNCETFQARRMQARFRNAQGKPELVHTLNGSGLA
                     VGRTLVAILENYQRADGGVDVPAALRSYMGGASIIAAA"
BASE COUNT          485 a          874 c          925 g          460 t
ORIGIN      
        1 cgttcggttc gttcgagcgc aacgcagccg tcgacaccgc cttgttccga ttcgtgccgc
       61 cgccaggcgc cgatgtcgtc ggcgaatgac ctttttgccg tagtcaatcc ggcgactccg
      121 ctggccgagc gcctgcggcc gaaaagcatc gccgaggtca tcgggcagag gcacctgctc
      181 ggaccgggca agccgctggc ggtggccttc gcgtccggca agccgcattc gatgattctc
      241 tggggcccgc cgggcgtcgg caagacgacg ctcgcgcgcc tgatggccga tgcgttcaac
      301 gcggaattca tcgcgttgtc ggcggtgctt tcaggcgtca aggacattcg cgacgcgatc
      361 gcgcaggcgg aggcgacgct tgcgcaatcg ggccgtcaca cgatcctgtt cgtcgacgag
      421 gtgcatcgct tcaacaaggc acagcaggac gcctttcttc cgttcgtcga aaaaggcctg
      481 gtcactttca tcggcgcgac gaccgagaat ccgtcgttcg aggtcaacag cgcgttgctc
      541 agtcgcgcca ccgtctatgt gctcgaggcg ctgtcggccg acgagctctc ggcgctgctc
      601 gatcgagcgt tgccggcggc tatgctcggc gtcgcgctca acgccgccgc acgagacgcg
      661 gtaatcgcct atgccgacgg cgacggccgg cgcctgttga atctggtcga gcaactggcc
      721 gtcgccgcgc aggaagcggg tcggcgcgag atcgacagcg aattcgtcga gacggcgatc
      781 acgcggagtc tgcggcgctt cgacaagggc ggcgaggcgt tttacgacca gatatcggcg
      841 ttgcacaagt cggtccgcgg atccgatccc gatgcggcgc tctactggtt ctgccggatg
      901 ctcgacggcg gcgccgaccc gctgtatgtc gggcggcgga tgatacgaat ggccagcgaa
      961 gacatcggac ttgccgatcc gcgcgcgctg cagctgactc tggacgccgt cgcgacctac
     1021 gaacgcctcg gcacgcccga gggcgagctc gcgttggcgc aggccgtgat ctatctcgcc
     1081 tgcgcggcca aaagcaatgc agtgtacgtc gcgtacggcg ccgcgcgcgc gttcatcgcc
     1141 gaagatggct cgcgcccggt gccgctgcac ttgcgcaatg cgccgaccag gctgatgaag
     1201 aatctgggtt acgggaagga ctatcgctac gctcacgacg aggaagatgc ctacgccgcc
     1261 ggcgaaagtt atctgcccga gggcatggca cggcccggct ggtatcgacc gaccgatcgc
     1321 ggcctcgaag cgaggatcgc ggaaaggctt gccgagttga ggcatcgcga cgccaaggcg
     1381 cgctcgggcg gcgaaggcaa ctcctgaccg cgggtgctgc ctggcggtgt gcagccctct
     1441 gctaaaattc gcgcatgatc gatatccagc ttctgcgcaa cgacctcgat gacgtcgtcg
     1501 cgcgtctgaa gacgcgtgga tatcagctcg atgggcccgc gttcgtcgag ctcgaggctg
     1561 agcgcaagca tatacagatg cgcacccagg agctccaggc gaggcgcaac acggtctcga
     1621 aagagatcgg cattgccaag agcagaggcg aagacgcgtc tgcgacactg cgcgaggtcg
     1681 ccggcatcgg cgacgagctg acttcgctcg aacgcaatct cgaggccgtg cagcagcgcc
     1741 tccgcgattg gctgctaacg atgcccaacg tgccgcatgc gagcgttccg gtcgggacct
     1801 ccgaggccga caacgccgaa gtccgccgct ggggtagccc gaggaagttc gactttgcgc
     1861 cgcgcgatca cgtcgacatc ggcgcggcgg ttggcggact cgacttcgac gtgtcggcga
     1921 aaatttccgg cgcccgtttt tatgcgctgc gcggagcggt cgcgcgcctg caccgggcgc
     1981 tggtgcagct gatgctcgat acgcacacga ccgagcacgg ctacgcggaa tgctatgtgc
     2041 cctatctggt caatgccgac gcactgatcg gcaccggcca gctgccgaaa ttcgaggagg
     2101 acctgttctg ggcggcgaag ggcggcgcaa ctgacgagga gcgctggtac ctgatcccga
     2161 ctgccgaggt gccactggcc aatttcgtgc gcgagcagat cgtcgcggct acggcgctgc
     2221 caatccggct caccgcgcac tcgccgtgct tccgctcgga ggcgggaagt tacgggaagg
     2281 acacccgagg catgatccgc gtccaccaat tcgacaaggt cgagatggtg cagatcgtcg
     2341 aaccggaccg ctcgtacgac gcactcgagg aaatggtgca gcacgctgaa gcgatcctgc
     2401 aaaagctcga gctcccgtat cgcgtcgtga ccctgtgcac cggcgacatg ggcttctccg
     2461 cggcgaagac ctacgatctg gaagtatggc tgccggggca gggcaagtat cgggagattt
     2521 cgtcgtgctc gaattgcgag acatttcagg cgcgccgcat gcaggcgcga tttcgcaacg
     2581 cgcaaggcaa gcctgagctc gtgcacacgc tgaacggatc gggtctggcc gtcggcagga
     2641 cgctggtcgc gatcctcgaa aactaccagc gcgccgacgg cggcgtcgac gtccccgcag
     2701 cgctgcggtc gtatatgggt ggcgccagca tcatcgcggc ggcg
//
LOCUS       DATANS010000487         2225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_66211, whole genome shotgun sequence.
ACCESSION   DATANS010000487 DATANS010000000
VERSION     DATANS010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66211"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2038
                     /locus_tag="VHT22_07210"
     CDS             <1..2038
                     /locus_tag="VHT22_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX3137380.1"
                     /translation="FVVGDPMQSVYGFREAEVGLYLDAQRQRRLGEVALEPLVLQCNF
                     RSQCDLVGWVNRVFAQAIDESPRPTEGVVAFRTADPVLPAEPPPAVTVEACASDADEA
                     AKVVSHVRTALADRAEVAILVRKRGDLAEILPALRGAGIPFAAVDLDRMADRQTILDL
                     ASLTHALIQPDDRLAWLAVLRAPWCGLTLPDVFALDGAAGGPSLCEGIVEHQASAPGV
                     PASMSPDGARRLQRLAAAIAPAVADRGRMPLAAAVRGVWLALGGPACIGEASDLEAAE
                     RFFTLLAEHAPGADVPDWNAFLDALAATFIETGAGADAKVRIMTLHRAKGLEFDVVVM
                     PGLARAPGSGAPQLMRWRRRAGGLLLAPIAARHVDLAEDDPVYGYLNKLAASEEAAEL
                     VRLLYVGCTRARKRLHLTTVLGIEPGDNARLRWRKPARGTALSALWAAIEADLPPPPS
                     VAPGATPSSASNGVPLLRLPLDWRPPPLPPALPIASRAAAADEEVVEFDWVRETARQI
                     GIVAHRLLRQCADEGLERWSPERIAAQRARVVRELAGLGFTGAEAEAAADQVLGAVAS
                     TLADARGRWLFDPRHTDAHSEYALTGIRDGAPVHVVLDRSFVDADGVRWIVDFKLSQH
                     QGAGRDLFLDSEQARYRAQLDGYAEVVRGLGTQSIRLGLYFPLLAGWREWEGPR"
BASE COUNT          327 a          747 c          757 g          394 t
ORIGIN      
        1 gtttgtcgtc ggcgatccga tgcagtcggt ctacggcttt cgagaagcgg aggtcggctt
       61 gtatctggac gcgcagcggc agcggcggct gggcgaagtc gcgctggagc cgctggttct
      121 gcagtgcaat tttcgctcgc aatgcgatct cgtcggatgg gtcaaccggg tcttcgctca
      181 ggcgatcgac gagtcgccgc gtccgaccga aggcgtcgtc gcgtttcgca ccgccgaccc
      241 ggtgctgccg gcagagcctc cgcccgccgt tacggtcgag gcttgcgcta gcgacgccga
      301 tgaagcagcg aaggtagtct cgcatgttcg caccgcgctg gccgaccgcg ccgaggtcgc
      361 gattctcgtg cgcaagcgtg gcgacctcgc ggaaattctt cccgctttgc gcggagccgg
      421 cattccgttc gctgcggtcg atctcgatcg gatggcggat cgccagacga tcctcgatct
      481 cgcatcgctg acgcacgctc tgatccagcc cgatgaccgg ctcgcatggc ttgcggtgct
      541 gcgcgcgcca tggtgcgggc tgacccttcc cgatgttttc gcgctcgacg gcgcggcagg
      601 cgggcccagc ctgtgcgaag ggatcgtcga gcaccaggcg tcagcgccgg gggtacccgc
      661 cagcatgtct cccgatgggg cacgtcgctt gcaaagattg gctgccgcga tcgcacccgc
      721 cgtcgccgat cgcggccgca tgccgctcgc tgcagccgtc cggggagttt ggctcgccct
      781 cggcggaccg gcctgcatcg gggaggcaag cgatctggaa gctgccgagc gatttttcac
      841 actgctggct gaacacgctc ctggcgccga cgtccccgac tggaacgcct ttctcgatgc
      901 gctggccgcc acgttcatcg agacaggggc tggcgccgac gcgaaggtgc gcatcatgac
      961 cttgcatcgt gcgaaagggc tggaattcga cgtagtagtc atgcccggtc tggcgagggc
     1021 gccgggtagc ggggcaccgc aactgatgcg ctggcgccgt cgggccggcg gactgctgct
     1081 ggcgccgatc gccgcaaggc acgtcgacct cgccgaggac gacccggtct acggctacct
     1141 gaacaaactc gctgccagcg aggaagccgc cgaactcgtt cggctgctct acgtcggttg
     1201 cacgcgcgcc agaaagcgat tgcacttgac cacggtgctc ggcatcgaac cgggcgacaa
     1261 tgcgcgactg cgctggcgaa agcctgcgcg cggcaccgcg ctttccgcgc tttgggccgc
     1321 gatcgaagcc gatcttccgc cgccgccttc ggtcgcgcct ggcgcgacgc cttcgagtgc
     1381 gtccaacggc gtgccactgc tgcgcctgcc gctcgactgg cggccgccac cgctgccgcc
     1441 cgcgctgccg atcgcatcgc gcgcggcagc cgccgacgag gaagtggtcg aattcgactg
     1501 ggtccgcgaa accgcgcgcc agatcggcat cgttgcccat cggctgttgc gccaatgtgc
     1561 ggacgagggc ctcgagcgat ggtcgcccga gcgcatcgcc gctcagcgcg cgcgcgtggt
     1621 gcgcgagctc gcaggtctgg gatttaccgg cgccgaagcc gaagccgccg ccgatcaggt
     1681 gctcggcgcg gtcgcctcga ccttggccga tgcgcgcggt cgctggctgt tcgatccgcg
     1741 acacaccgat gcgcacagtg aatacgcgtt gaccggcatt cgcgacgggg cgccggtcca
     1801 tgtcgtgctc gatcggagct tcgtcgacgc cgatggcgtg cgctggattg tcgatttcaa
     1861 gctttcgcaa caccagggtg cgggccgcga tctttttctc gacagcgagc aggctcgcta
     1921 ccgggcgcag ctggacgggt atgccgaagt cgtgcggggc ttgggcacgc aatcgattcg
     1981 tctcggcctt tatttccccc tgctcgcagg ctggcgggaa tgggaaggcc cgcgctgacc
     2041 caaaagcgcg gagctccgag cgctggaccg gaaccggtcg cacaggctaa aatcaaggtt
     2101 tttcaaggtc ttccgacctt tggacgggaa ctgaagtgca gttatttcgt gggctcccgg
     2161 attccgccga ccgccaccgc cccatcgcgc tgaccatcgg caacttcgac ggcgtccatc
     2221 gtggc
//
LOCUS       DATANS010000488         1826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_403876, whole genome shotgun sequence.
ACCESSION   DATANS010000488 DATANS010000000
VERSION     DATANS010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403876"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VHT22_07215"
     CDS             complement(<1..915)
                     /locus_tag="VHT22_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX3137381.1"
                     /translation="MKVSSGVTTFLFTDIEGSTRLWDREHERMQIALARHDAIVRAAM
                     EDNHGIVVKMSGDGAHGAFDDPLDALGASLQLQQGLADPAATHGIALPVRCGLHAGVT
                     ERRDNDFFGGVVNRAARIMSVAHGGQVLLSQAVATLLGERLPPDAALRDLGTVRLRGL
                     ESPERVFQLMHPCLRQDFPSLRSLEVTPNNLPQQLSSFVGRERELAEIAKLLGSTRLL
                     TLTGAGGLGKTRLTLHIAADLLDQFSDGVWFVELAPLSDARLAPQAVASALGVTEEPG
                     RPVIEALIKLVADRRMLLILDNCEHLIDA"
     gene            complement(951..>1826)
                     /locus_tag="VHT22_07220"
     CDS             complement(951..>1826)
                     /locus_tag="VHT22_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3137382.1"
                     /translation="WWFVAFAPLAYLLPVLLLASVWFTLSWIWRTPRAPEYRLGPAAC
                     ARLFLGEVSAVALSWPLMVAHRLLIADPVAAPSRLPLLLVHGVAVNDGVWFALRRDLA
                     RRGLGPVYTINYGPPLAGIEHFAGQLASRIDAVCAATGAQRVALIAHSMGGLVSRAYL
                     RRFGAARIEQLITIGTPHHGSMLAWTFVGQCLAQMRPGSRWLVEINRAESEPPPVPIT
                     SIRSRHDSMVAPQASADLACAHNIALTGIGHNALLNHRAVMDEVARALASGREGGSQP
                     SPEPSARLGKPMLRR"
BASE COUNT          332 a          622 c          604 g          268 t
ORIGIN      
        1 ggcatcgatc agatgctcgc agttgtccag gatcagcagc attctgcggt ccgcgaccag
       61 cttgatcagg gcctcgatca ccggccggcc cggctcttcg gtcacgccca gcgccgatgc
      121 cactgcctga ggcgccagcc gcgcatccga caaaggggca agctcgacga accaaacgcc
      181 gtcggaaaac tggtcgagca agtcggcggc gatatgcaat gtgagccgcg tcttgccgag
      241 acctccggca ccggtcagtg tcaagagccg cgtgctgccg agaagctttg cgatctcggc
      301 gagctcccgc tcgcggccga cgaaggaact cagctgctga ggcaggttgt tcggcgtaac
      361 ctcgagtgaa cgcagcgacg gaaagtcttg tcgcaggcac gggtgcatga gctggaacac
      421 gcgttcggga ctttccaggc cgcgcaggcg cacagtaccc aagtcgcgca atgcggcgtc
      481 cggcggaagg cgctcgccga gcagcgtcgc gaccgcctgc gacaacagga cctggccgcc
      541 gtgcgcgacg ctcatgatgc gcgcggcgcg gttgacgact ccgccgaaga agtcattgtc
      601 gcgtcgctcg gtcaccccgg cgtggagtcc gcagcgcacg ggtaatgcaa taccgtgcgt
      661 cgctgccgga tcggccagcc cctgctgcag ctgcagcgag gcgccgagcg catcgagcgg
      721 atcgtcgaac gcgccgtgtg cgccgtcgcc gctcattttc accacaatgc cgtggttgtc
      781 ctccatcgcg gcgcgcacga tcgcatcgtg gcgcgcaagc gcgatttgca tgcgctcgtg
      841 ctcacgatcc cacaggcgcg tgctgccctc gatgtcggtg aacaggaatg tcgtgacgcc
      901 ggatgagact ttcacgtcgg tgcgcctcga atagcctggc tggcgaaagt ctagcgtcgc
      961 agcatcggct tacccagccg tgcggatggc tccggcgacg gttgggagcc gccctccctc
     1021 ccgctcgcaa gagcgcgtgc gacctcgtcc atcacggcgc gatggttcag caaggcattg
     1081 tggccgatcc ctgtcaacgc gatgttgtgc gcgcaggcga gatcagcact cgcctgcggt
     1141 gccaccatgc tgtcgtgacg cgaccggatc gaagttatcg gcaccggcgg cggctcgctt
     1201 tccgcacgat tgatctcgac cagccagcgg ctgcccggcc gcatctgcgc caagcattgt
     1261 ccgacgaagg tccaggccag catgctgccg tgatgcggcg tgccgatggt gatcagctgc
     1321 tcgatgcgcg ccgcgccgaa acgtcgcagg tatgcgcggc tgaccagccc gcccatgctg
     1381 tgcgcgatca gcgcgacgcg ctgcgcgccg gtggccgcgc agaccgcatc gattcgggaa
     1441 gcgagctggc cggcgaaatg ctcgatgccg gcaagcggcg gcccgtagtt gatcgtgtac
     1501 accggtccca agcctcgtcg cgcgagatcg cgacgcaagg cgaaccagac gccatcgttg
     1561 accgccacgc cgtgcaccaa caacaacggc agccgcgagg gcgcagcgac cggatcggcg
     1621 atcagcaagc gatgcgcgac catcagcggc cacgacagcg cgacggcact gacctcaccc
     1681 aggaacagcc gagcgcacgc ggccgggccg aggcgatatt caggcgcccg cggcgtgcgc
     1741 cagatccagc tcagcgtgaa ccacaccgac gccagcaaca acaccggcag cagataggcg
     1801 agcggggcga aggcgacgaa ccacca
//
LOCUS       DATANS010000489         1689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_108517, whole genome shotgun sequence.
ACCESSION   DATANS010000489 DATANS010000000
VERSION     DATANS010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108517"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /locus_tag="VHT22_07225"
     CDS             <1..1353
                     /locus_tag="VHT22_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137383.1"
                     /translation="AHDPELFSKLCDGVRHEDEQICLTYARMLSNTFPDKAPEIIFER
                     MQHASAPVCDQLVRLIGPKLPEELLHRLEATRERGDAHERATVLATRFEAQDPRMKPE
                     VDKCLASDNPRLIACGVQGVLSYGMNDRLDGAFARWRGLLEGPQTSSIYAGLHLGPRV
                     AMPAPFLPILCGLLEHVEDGVKKSALAALQRGAFTADARLTALLESLVQSPDQQMRMA
                     SISCYRLLPAADRERLAFAALTDRHPNVVGAALAVLEECIENLPERLFAWLDESCAPP
                     RQQQQVVCYLSRHGALRQPFEEFAGRRLQAAAQMAQALHVLEAAEAGRKDAGWTLMKI
                     VLAERFTQTLEVALLAMENLGDSHSIRIVYLALKSKDKRQVARAREALTNIANTELGT
                     QLGQLLIVASDHQAVIDTVPGATAFASAADALQWCANHVDYWLKECAQHALAAAAARG
                     "
     gene            1361..>1689
                     /locus_tag="VHT22_07230"
     CDS             1361..>1689
                     /locus_tag="VHT22_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX3137384.1"
                     /translation="MADFFDRIVILKRTPIFSEVATDDLRVVVEEMQEEAYFKGERVF
                     EINDPSDRMYIVLTGKIGISINPDPKVEDFVSIVEEGGCFGEMGPLDGSPRSGTAHVI
                     EDSQLLYL"
BASE COUNT          316 a          525 c          544 g          304 t
ORIGIN      
        1 gcgcacgatc cggaactttt ctcgaagctt tgcgacggcg ttaggcacga agacgagcag
       61 atctgcctca cttacgcacg catgctgtcg aacacatttc ccgataaagc gcccgagatc
      121 attttcgagc gcatgcagca cgcgagcgcg ccagtgtgcg atcagctggt ccgtttgatc
      181 ggcccgaaac tgccggaaga attgctgcac cggctcgagg cgacacgcga gcgcggcgat
      241 gcgcacgagc gcgcgacagt cttggcgacg cgcttcgaag cgcaagaccc gcgtatgaaa
      301 cccgaggtcg acaaatgtct ggcttcggac aacccgcgcc tgatcgcctg cggcgtgcag
      361 ggtgtgctga gttacggcat gaacgaccgg ctggatggcg cgttcgcgcg ctggcgcggc
      421 ctgcttgagg gcccccagac ctcgtcaatt tatgcagggc tgcatctcgg cccaagggtt
      481 gcgatgccag cgccgttcct gccgattctg tgtgggctgc tcgagcatgt cgaagatggc
      541 gtcaagaagt cggcgctcgc ggccttgcag cgcggagcct tcaccgcgga cgcgcggctg
      601 accgcgctgc tcgaatcgct ggtccaatcc ccggaccagc aaatgcgcat ggcgtcgatc
      661 tcgtgctatc gcctgttgcc ggcagcggac cgcgaacggc tcgcctttgc cgccttgacc
      721 gaccgccatc ccaatgtcgt cggcgcggcg ctcgctgtgc tcgaggaatg catcgaaaat
      781 ctgcccgagc ggctgtttgc atggctagac gaatcgtgcg cgccgccgcg ccagcagcag
      841 caggtggtct gttatctctc gcgtcacggg gcgctgcgcc agccgttcga ggagttcgca
      901 ggacgtcgcc tgcaggctgc tgcccagatg gcgcaggcgc tgcacgtgct tgaagcggcc
      961 gaagcggggc gcaaagatgc gggctggacg ctgatgaaga tcgtgctcgc cgagcgcttc
     1021 acgcagacgc tcgaagtcgc attgctcgcg atggaaaatc tcggcgactc acacagcatc
     1081 cgcattgtct acctcgcgct gaagagcaaa gacaagcgcc aggtcgcgcg cgcgcgcgaa
     1141 gcgctgacca atatcgcaaa taccgagttg ggcacgcagc tcggccagct cttgattgtg
     1201 gcgagcgacc atcaagccgt gatcgacacg gtgcccggcg cgacggcgtt tgcgagcgcc
     1261 gcggatgcac ttcaatggtg cgccaatcat gtagactact ggcttaaaga gtgcgcgcag
     1321 cacgcgctcg cggccgcagc cgccagggga taagcagcgc atggccgatt ttttcgatcg
     1381 tatcgtcatc ctgaaacgaa cgcccatatt ttctgaagtc gccaccgacg acctgcgcgt
     1441 ggtggtggag gagatgcagg aggaggccta cttcaagggc gagcgcgtgt tcgagatcaa
     1501 cgatccctcc gaccgcatgt atatcgtgct gaccggcaag atcggcatct caatcaatcc
     1561 cgatccgaaa gtcgaggact tcgtaagcat cgtggaggag ggcggctgct tcggcgagat
     1621 gggcccgctc gacggctcgc cgcgctcggg caccgcccac gtgatcgagg attcgcagct
     1681 tctgtatct
//
LOCUS       DATANS010000490         1644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_66460, whole genome shotgun sequence.
ACCESSION   DATANS010000490 DATANS010000000
VERSION     DATANS010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66460"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="VHT22_07235"
     CDS             <1..1079
                     /locus_tag="VHT22_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3137385.1"
                     /translation="IFRKLYAEDRDLTIRRDTWGFFLGLIATATLYAAYAWIAISAIA
                     TRITLGQMTMYLMLFRQGQSAVSAILSAVGGMYEDNLYLSTLYEYLETPVDSTPGTVV
                     HGPHPEDGVRFEQVSFRYPGAEQLSLVDIDLHVKPGESLALVGENGSGKTTLIKLLTR
                     LYQPTAGRILLDGKDLREWDEAALRQRVGVIFQDFARYQLLLGENIGAGDVQHFEDEP
                     RWREAGEKGMAQPIVDTLPDGYRTPLGKWFNDGRELSGGQWQKVALSRAFMRSRADIL
                     VLDEPTAAMDAGAEATVFEHFRTLTRGKIAILISHRFSTVRMADQIVVIQEGRIVERG
                     SHEELMRVNGHYAHLFSLQAKGYR"
     gene            complement(1084..>1644)
                     /locus_tag="VHT22_07240"
     CDS             complement(1084..>1644)
                     /locus_tag="VHT22_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137386.1"
                     /translation="HREGSMKRAAIAVVLLVVLGACHAGDGQTSSDASALTGELWVYL
                     EDAWQRPPPQSGLIGFVPARVVRFDADGTMSLISCALLRTERETFISPGDGLAFYFGK
                     WHAAKDGFVVDYVKTSETIHASSTGLPFERKLSATVVLGGGGGLVFDGTRFERKDAPS
                     MESYNEFRDAQRREHAGEIDQFLNEH"
BASE COUNT          297 a          529 c          516 g          302 t
ORIGIN      
        1 ccatctttcg caagctctac gccgaggatc gcgatctgac gatccgccgc gacacgtggg
       61 gcttctttct cggtctcatc gcgaccgcga cgctgtacgc cgcgtacgca tggatcgcga
      121 tctcggcaat cgccacgcgc atcacgctcg gccagatgac gatgtacttg atgctgttcc
      181 gtcaaggtca gtcggccgtc tcggcgattc tctctgcggt cggcggcatg tacgaagaca
      241 atttgtattt gtcgacgctc tacgaatatc tcgagacacc tgtcgacagc acgccaggca
      301 ccgtcgtgca tggaccgcat cccgaagatg gtgtgcgctt cgagcaggtg agctttcgct
      361 atcccggcgc ggaacaactg tcgctcgtcg atatcgatct gcacgtcaag ccaggcgaat
      421 cgctcgcgct ggtcggcgag aacggctccg gcaagacgac gctgatcaag ctgctcacgc
      481 ggctctatca gccgactgcg ggccgcatcc tgctcgacgg caaagacctg cgcgagtggg
      541 acgaggcggc gctgcgtcag cgcgtcggcg tgatcttcca ggacttcgcg cgctaccagc
      601 tgctgctcgg cgagaatatc ggcgccggcg acgtgcagca cttcgaagac gagccgcgtt
      661 ggcgcgaggc cggcgagaaa ggcatggcgc agccgatcgt cgacacgttg ccagacggct
      721 atcgcacgcc gctcggcaaa tggttcaacg acggccgcga gttgtcgggc ggtcagtggc
      781 agaaagtcgc gctgtcacgc gcgttcatgc gttcgcgtgc cgacattctc gtgctcgatg
      841 agccaacggc ggcgatggac gcaggcgcgg aggccacggt gttcgagcat ttccgcacgc
      901 tcacgcgcgg caagattgcg attctgatct cgcatcgatt ctcgacagtg cggatggcgg
      961 accagatcgt cgtgatccag gagggtcgca tcgtcgagcg cggcagccac gaggagctga
     1021 tgcgcgtgaa cgggcactat gcgcatctat tctcgctgca ggcgaagggg tatcggtagg
     1081 ccgctagtgc tcgttcagga actggtcgat ctccccggca tgctcccgcc tctgcgcatc
     1141 cctgaactcg ttgtagctct ccatggacgg tgcgtccttg cgctcgaagc gagtcccgtc
     1201 gaacaccaga ccgccgccgc cgccgagaac gacggtcgcc gatagttttc gctcgaacgg
     1261 caagccggtc gacgacgcgt ggatggtctc gctggtcttc acatagtcga cgacaaagcc
     1321 gtccttcgct gcgtgccatt tgccgaaata gaaggccagc ccgtcgccgg gactgatgaa
     1381 tgtctcgcgc tccgttcgca gcagggcaca tgagataagc gacatcgtgc catccgcatc
     1441 gaaacgcacc acccgcgccg gcacgaagcc gatcagcccg ctctgcgggg gcggacgctg
     1501 ccacgcgtct tccaggtaga cccacagctc gccggtcaag gcgctggcat cgctgctcgt
     1561 ctgcccgtcc ccggcgtgac aggcgccgag gacgaccaat agcacgacag ctatggcagc
     1621 gcgtttcatg gatccttcac gatg
//
LOCUS       DATANS010000491         1600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_24227, whole genome shotgun sequence.
ACCESSION   DATANS010000491 DATANS010000000
VERSION     DATANS010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24227"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VHT22_07245"
     CDS             <1..807
                     /locus_tag="VHT22_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3137387.1"
                     /translation="PEQIENIRRQLGVDQPYYVQLWIFLKQIATGDFGVSWATNERVS
                     AIFATRLGPSLTVLLPLLVIDTLLAMAAAMLVAYMRGSLTDRLVMIGCTVGQSVSILV
                     YIIVLQYVLAYQLGWFPVQGWGNNLAENLFRFSLLPIIVGVVVSLAPDTRLYRSFFLD
                     EINQDYVRTARAKGMGENRVMWVHVLRNASIPIITNVMIQLPGLLAGAFLIERFFSIP
                     GIGREVILAVERSDFPVIKAITIYVAIATMVINLLADLLYKAVDPRVQLK"
     gene            804..>1600
                     /locus_tag="VHT22_07250"
     CDS             804..>1600
                     /locus_tag="VHT22_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3137388.1"
                     /translation="MSELVIAGRNVPRARSPGLWVLAWRRFGRDRVGVASLAVVVFFC
                     ALMTASGLNLIAADWAQEAGVNYAPPTFMGPDAVEAEAVTPPVAQPAGGERAESSDIV
                     DPLADVLAEIRRDPAAVAPPAPKAATLPFGGDKWGRDVLKKVIKGTQTSMFVGIAAAL
                     LATLLGTVFGALAGYYGRAIDDFFNWFYSIFTSVPYLLLILAIAAVLNQKGTLTVVLI
                     LGLTGWPGIFRLVRAEYLKHKAREYIQAADAIGASHRRRMFVHILPNV"
BASE COUNT          256 a          505 c          517 g          322 t
ORIGIN      
        1 cccgagcaaa ttgaaaacat ccggcgccag ctcggcgtcg atcagccgta ttacgtgcag
       61 ctgtggatat tcctgaagca gatcgcgacc ggcgacttcg gcgtgagctg ggcaaccaac
      121 gagcgagtgt cggcgatatt cgcgacccgg ctcggtccat cgctgaccgt gctgctgccg
      181 ctgctcgtga tcgatacgct gctggccatg gctgctgcca tgcttgtcgc ctatatgcgc
      241 ggctcgttga ccgatcgcct ggtgatgatc ggctgcacgg tcggccagtc ggtcagcata
      301 ctcgtgtaca tcatcgtgct ccagtacgtg cttgcgtacc aacttggctg gtttccggtc
      361 cagggttggg gcaacaacct cgccgagaac ctattccggt tcagcctgct gccgatcatc
      421 gtcggcgtgg tggtgtcgct cgcgccggac acgcggctct accgcagctt ctttctcgac
      481 gaaatcaacc aggattacgt gcgcacggcc cgcgccaagg gcatgggtga aaatcgcgtg
      541 atgtgggtgc acgtgctgcg caacgcgtcc atccctatta tcaccaacgt gatgatccag
      601 ttgccggggc tgctcgcggg tgcattcctg atcgagcgct ttttctcgat tccgggcatc
      661 ggtcgcgaag tcatcctggc ggtcgagcgc agcgattttc cggtgatcaa ggcgatcaca
      721 atctacgtcg cgatcgcgac catggtcatc aatctgctgg cggaccttct gtacaaggcg
      781 gttgacccgc gggtgcagct caaatgagcg aactggttat tgcaggccgc aacgtgccgc
      841 gcgcccggtc gccgggcctg tgggtgctcg cgtggcggcg tttcggccgc gaccgcgtcg
      901 gcgtagcgtc gctcgcggtg gtggtgtttt tctgcgcgct gatgaccgcg tcgggactga
      961 atttgatcgc cgccgactgg gcgcaggaag ccggcgtcaa ctatgcgccg cccaccttca
     1021 tggggccgga cgcggtagaa gcggaggcgg ttacgccgcc ggtcgcgcaa cccgccggcg
     1081 gcgaacgcgc agagtcatcg gacatcgtcg atcctttggc cgacgtgctc gccgaaataa
     1141 ggcgcgaccc cgctgcggtg gcgccgccgg cgcccaaagc cgcgacgctg ccgtttggcg
     1201 gcgacaagtg gggccgcgac gtgctgaaga aggtgatcaa gggcacccag acctcgatgt
     1261 tcgtcggcat cgcggcggcg ctgctggcga ccctgctcgg caccgtgttc ggcgcgcttg
     1321 ccggctacta cggtcgcgca atcgacgatt tcttcaactg gttctacagc atcttcactt
     1381 ccgtgccgta cctgctgctc atactcgcga tcgccgcggt gctgaaccag aaaggcacgc
     1441 tgacggtggt gctcatcctc ggtttgacgg gctggccggg cattttcagg ctggtgcgcg
     1501 ccgagtatct caagcacaag gcgcgcgaat acatccaggc ggccgatgcg atcggcgcct
     1561 cgcaccggcg gcgcatgttc gtgcacatcc tgcccaacgt
//
LOCUS       DATANS010000492         1797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_194728, whole genome shotgun sequence.
ACCESSION   DATANS010000492 DATANS010000000
VERSION     DATANS010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194728"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VHT22_07255"
     CDS             complement(<1..820)
                     /locus_tag="VHT22_07255"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX3137389.1"
                     /translation="MSAIVDVIAREILDSRGNPTIESDVVLESGISGRAAVPSGASTG
                     SKEAIELRDGDAARYGGKGVQKAVEHVNTEICEAIIGLDASEQAFIDRTMIELDGTDN
                     KARLGANAILAVSCAVAKAAAEESTLPLYRYLGGAGEMALPVPLMNVINGGAHANNSV
                     DMQEFMLVPLGAPTFREALRYGAEVFHALKKLIDAKGMATTVGDEGGFAPNLPSNEAA
                     LQLLLEAIDKAGYTPGTDIALALDCAASEYYKDGMYELESEGAKLTRSQMVDYLA"
     gene            complement(855..1700)
                     /gene="kdsA"
                     /locus_tag="VHT22_07260"
     CDS             complement(855..1700)
                     /gene="kdsA"
                     /locus_tag="VHT22_07260"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX3137390.1"
                     /translation="MNLCGFEVGLNRPLFLIAGPCVVESEQLQIDVAGQLKEICAALS
                     IPFVFKSSYDKANRSSSQSFRGVGMQEGLRILSEVKRQIGVPVLTDVHAEDEIAEAAA
                     VVDVLQTPAFLCRQTDFIHAVAAAGKPVNIKKGQFLAPEDMKNVVDKAREASGRDNIL
                     VCERGASFGYHNLVSDMRALAIMRSTGCPIVFDATHSVQLPGGQGATSGGQREFVPVL
                     ARAAVAAGVAGVFMETHPDPAQALSDGPNAWPLGRMRELLTTLAELDATVKRRGFSEA
                     SLLAQ"
BASE COUNT          292 a          606 c          566 g          333 t
ORIGIN      
        1 ccgccagata gtcgaccatc tgcgatcggg tgagcttcgc gccttccgat tcgagctcgt
       61 acatcccgtc cttgtagtac tcggacgcgg cacaatcgag cgccagcgca atgtcggtgc
      121 cgggggtgta gccggccttg tcgatcgcct cgagcagcag ctgcagcgcg gcctcgttcg
      181 atggcaggtt cggcgcgaat ccgccctcgt cgccgaccgt cgtcgccatg cctttggcgt
      241 cgatcagttt cttcagcgcg tggaaaacct cggcgccgta gcgcagcgcc tcgcgaaatg
      301 tcggcgcgcc aagcgggacc agcatgaatt cctgcatgtc gacgctgttg ttcgcgtgtg
      361 cgccgccgtt gatcacattc atcaacggca ccggcagcgc catctcgccg gcaccaccca
      421 gatagcgata gagcggcaac gtcgattcct cagccgccgc cttggccacc gcgcaggaga
      481 cggcgagaat cgcgttggcg ccaagccttg ctttgttgtc ggtgccgtcg agttcgatca
      541 tcgtgcgatc gatgaacgcc tgctcggagg cgtcgagccc tatgatcgct tcgcagattt
      601 cggtgttgac gtgctcgact gccttttgca cgcccttgcc gccgtagcgc gcggcgtcgc
      661 cgtcacgcag ctctatcgct tccttcgacc cggtcgacgc gcccgaggga accgccgccc
      721 ggccgcttat gcccgattcg agcaccacgt ccgactcgat cgtggggttg ccgcgcgaat
      781 ccaggatctc gcgggcgatg acgtcgacga ttgcgctcat gctctgatcc gatggttgtc
      841 gttggagttc tgggtcactg cgccagcagt gacgcttcgg agaagccccg ccgcttgacg
      901 gtcgcatcga gctcggccag cgtcgtcagc aattcgcgca tccgtccgag cggccacgca
      961 ttcgggccat ccgacaacgc ctgcgccggg tccggatgcg tttccatgaa taccccggcg
     1021 acgccggcgg cgacggcggc ccgcgccagc accggcacga actcgcgctg cccgccggag
     1081 gtcgcgccct gcccgccggg aagctgcacc gaatgggtcg catcgaacac gatcgggcag
     1141 ccggtcgagc gcatgatcgc gagcgcgcgc atatccgaga ccaggttgtg ataaccgaag
     1201 ctcgcgccgc gctcgcaaac gaggatgttg tcgcggccgc tggcctcccg ggccttgtcg
     1261 acgacgttct tcatgtcctc gggggcgaga aactggccct ttttgatgtt gaccggcttg
     1321 cccgctgccg ccaccgcgtg gatgaagtcg gtctggcggc agaggaatgc cggcgtctgc
     1381 agcacatcga cgaccgcagc ggcttcggcg atctcgtctt cggcgtgcac atcggtcaac
     1441 accggcacgc cgatctgccg cttgacctcg gagaggatgc gcaggccctc ctgcatgccg
     1501 acgccgcgaa aagattgcga cgagcttcga ttggccttgt cgtacgacga tttgaaaacg
     1561 aatggaatgc tcaatgccgc gcagatttcc ttcaactgtc cggcgacgtc gatctgcagc
     1621 tgctccgact cgacgacgca cggcccggca atgaggaaca gaggccggtt cagcccgacc
     1681 tcgaatccgc acagattcac gagagataac gcctacgcga cgagttgcgc gcgcgtgcgc
     1741 ggcgtttcgc tttgcgccgc acgctgccgt tcgagcgccg ccttgacgaa ggcgatg
//
LOCUS       DATANS010000493         2316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_233472, whole genome shotgun sequence.
ACCESSION   DATANS010000493 DATANS010000000
VERSION     DATANS010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233472"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VHT22_07265"
     CDS             complement(<1..227)
                     /locus_tag="VHT22_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX3137391.1"
                     /translation="MFVRSAPASAFLLAGVAAACISIIATVANAAPSASQQEGLASVY
                     AKHFNGGNTASGERYDSRALTAAHRTLPLGT"
     gene            complement(293..1432)
                     /locus_tag="VHT22_07270"
     CDS             complement(293..1432)
                     /locus_tag="VHT22_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3137392.1"
                     /translation="MGANSPLGKTLALEPVEGSTPTVDEARPVAGASPAKADPQRELR
                     MTTADEFLAAAAKEYEAGTIDRVLWRQAADQHGRDVSLVIAAYLRARATALQARHKPA
                     ESAEIKLRGAGSNQDAMVRKVEMEPAVDIEPTELPRMGLRGVNPKILYSAGGVVAFFA
                     AAAIVYLVVSLRQSESVRPPVAAAATSSAAQPASPILIKSQQPRGATSSRAGTNEGGV
                     DALAATVRELKIAGNWNVLVLYANEWTRKEPKNPIAWRELSVGYANLQQFNDALDAAT
                     KAVELSPEDALSWRNLGQINLTVDRLHEADIAFGRALALNPDDADARCGAALVAQRQE
                     RPKDPNAFTRRVGPADGGCFDVSFASAAVVSTGGAARKPGSSAGR"
     gene            complement(1709..2194)
                     /locus_tag="VHT22_07275"
     CDS             complement(1709..2194)
                     /locus_tag="VHT22_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3137393.1"
                     /translation="MNPPQGSATSRNVSYADPRASDLVIHHMPGHFIRRLQQVAVKLF
                     FARVGLDMTPVQFAALAAVAHQPRIDQARLSDLIGYDRATIGGVIGRLESRGWLARSA
                     SRADRRVRLVRITPSGRKALARALPAVTSMQQVLLESLDAAERRHFERLCLKILAHHL
                     G"
     gene            complement(2191..>2316)
                     /locus_tag="VHT22_07280"
     CDS             complement(2191..>2316)
                     /locus_tag="VHT22_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HEX3137394.1"
                     /translation="IYHAKGVERLVRNSLWAGRTPDRFYDALEWLYGWTVEKCLA"
BASE COUNT          411 a          739 c          759 g          407 t
ORIGIN      
        1 tccgttccca atggcaaggt tcggtgcgcg gccgtcaggg cgcggctgtc gtagcgttct
       61 ccgctcgccg tgtttccgcc gttgaagtgc ttggcataga cggacgccaa gccctcctgt
      121 tgcgatgccg acggggcggc attggcaacg gtcgcgataa tggagataca ggcggcggct
      181 accccggcca atagaaaagc ggatgccggc gcacttcgga cgaacatggc cgacgttctc
      241 actatcgggg tggcacggca agaccgggtc tcggccggtt agacgctgca acttagcggc
      301 cggcggaaga ccctggcttt cgcgcggcgc cgccggtgga tacgacggcg gcgctggcaa
      361 agctcacgtc aaagcagccc ccatcggcag gtcctacccg ccgcgtaaac gcattgggat
      421 ctttcgggcg ctcctggcgt tgcgcgacca aagccgctcc acaacgcgca tctgcatcgt
      481 cggggttcaa cgccagagcc ctgccgaagg cgatatcagc ttcgtgcagg cgatcgacag
      541 tcaggtttat ttgtccaagg ttgcgccacg aaagcgcgtc ttccggcgac agctcgaccg
      601 cttttgtcgc ggcgtcgagg gcgtcgttga attgctgcag gttggcgtat ccgacactga
      661 gctcacgcca cgcgatcggg tttttcggtt ccttgcgggt ccactcgttt gcatagagta
      721 cgagcacgtt ccagttaccg gctatcttga gctcccgaac cgtcgcagcg agcgcgtcta
      781 caccgccttc gttggtgccg gcgcggctcg aagtcgcgcc ccgcggttgc tggctcttga
      841 tcagaatggg cgacgcaggt tgcgcagcag aggacgtcgc ggccgccgcg accggaggcc
      901 ggaccgactc gctttgccgc agtgaaacca ccaagtacac gattgcggca gcggcgaaga
      961 acgcgaccac accgccggcc gagtacagaa tcttcggatt gacgccgcgg agacccatgc
     1021 gcgggagttc ggtcggctcg atatctaccg caggttccat ctcgaccttt cgaaccatgg
     1081 cgtcctggtt cgagccggct ccgcgcaact tgatttccgc gctttccgct ggtttatgcc
     1141 gcgcttgcaa cgctgttgcg cgtgcgcgca gataagcggc aataaccaac gatacatccc
     1201 tgccatgctg gtccgcggct tggcgccaga gaacccggtc gatggttccc gcttcgtact
     1261 ctttggccgc ggcggcgaga aattcgtccg ccgtcgtcat ccgcagttcg cgttgaggat
     1321 ctgccttggc cggggacgcg ccggcgaccg gacgagcttc gtccaccgtg ggcgtcgagc
     1381 cttcgacagg ctcgagcgcc agcgttttgc cgaggggtga attggctccc atccgcgaga
     1441 cgacgcaagt gccgtgccag gcgagctgat gcgaacgaag ccgtcgtcta cagatgcgcc
     1501 cagaactcag caaaacgcct tggcgacttc cgctgcgcgg agtgaagcgc tcggcgcttg
     1561 cccgaggctt gacgaacctc aggtcaagag ctacaaaatg taatctacgc tgcgcacgat
     1621 gcgatggtga aacattgagc gtccgccggc caccagacgg caggccccgt gcggaaagcg
     1681 cagcgaaaag gcgcgattcg agccgccgct agcccaggtg atgcgcgagg attttcaggc
     1741 aaagacgctc gaagtgccgg cgctcggcgg catcgagcga ttccagcaag acttgttgca
     1801 tcgacgtgac ggcaggcagc gcccgggcga gcgccttgcg gcctgacggc gtgatgcgca
     1861 cgagcctgac ccgccggtcc gcgcgcgagg ccgagcgcgc gagccagccc cgcgattcga
     1921 gcctgccgat cacgcctcct atggtcgcgc ggtcgtagcc gatcaggtcg ctcaggcgtg
     1981 cctggtcgat gcgcggctgg tgcgcaaccg ccgcgagcgc cgcgaactgg accggcgtca
     2041 tgtcgagacc gacgcgagcg aagaaaagct tgaccgcaac ctgctgcagc cggcggataa
     2101 aatggcccgg catgtgatgg atgaccaggt cgctcgcgcg cggatcggcg tagctgacgt
     2161 tccggctagt ggcggaacct tggggcggat tcaagccagg catttctcga ccgtccagcc
     2221 gtagagccat tcgagcgcgt cgtaaaaccg atccggggtg cggcctgccc aaagcgaatt
     2281 gcgcaccagc cgctcgacgc ccttggcgtg atagat
//
LOCUS       DATANS010000494         3374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_320871, whole genome shotgun sequence.
ACCESSION   DATANS010000494 DATANS010000000
VERSION     DATANS010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320871"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VHT22_07285"
     CDS             <1..473
                     /locus_tag="VHT22_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137395.1"
                     /translation="DLKRQDDAAGKALTSLPEEREGKVVYDFLQSPLGAKLLELQNEA
                     WGRIVANVFTGEIDAWTLANKASLAKAVRAAAPGTPPPSDKTYLGKVTMDAGRGFTVT
                     VEHYATLRPVGSYVLNFSPNDLHWTDIAAAVPGIEPGQTRVLYRDTSGHLGDRP"
     gene            509..1147
                     /locus_tag="VHT22_07290"
     CDS             509..1147
                     /locus_tag="VHT22_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137396.1"
                     /translation="MTGAPLVLMFASLPACGWEDKSGADMPWLGGGGRAHLYHQRSSC
                     MGCPTGYQDEERWGGSAWSGKSPYATVGKAMEFLAENAGALRSARSIGAGAIGGAGGK
                     DGIIEPRALPYRMTVVMIEPTVVVMNFDLGAMIHEGEPQTGQIVGAAYIDGVVGYGTR
                     VPATGSLLWFSPEAKAAPAPVANQSSGKGEIRVGGVTLSLERGANYWTVTRR"
     gene            1269..2348
                     /gene="ada"
                     /locus_tag="VHT22_07295"
     CDS             1269..2348
                     /gene="ada"
                     /locus_tag="VHT22_07295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HEX3137397.1"
                     /translation="MKTTSKSTARAAATMADARWAAVIARDPAADGGFVYSVRTTGVY
                     CRPSCGARQARPEHVVFHLTAADAERAGFRPCLRCRPDQPPLAEQHAATVARLCRFIE
                     SAEQAPSLETLAVEAGLSVYHLHRVFKAVTGLTPKAYAAAHRARRVRAELDRSGTVTE
                     AIYGAGYNSNGRFYAQSNDVLGMTPSRYRAGGADTNIRFAIGECSLGAILVAASEVGV
                     CAILIGDDPDALARDLQDRFPRAELIGGDAGFEELVAKVVGLVEAPGLGLDLPLDVRG
                     TAFQQRVWQALREIPAGNTVSYADVAQRIGAPKSVRAVAQACGANALAVAIPCHRVVR
                     NDGALAGYRWGVERKRALLERESRR"
     gene            2345..3100
                     /locus_tag="VHT22_07300"
     CDS             2345..3100
                     /locus_tag="VHT22_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX3137398.1"
                     /translation="MNARDRAVAHVADIAAADAQTRLVSADWGEIASDLDARGSAVIE
                     RVISPAHSRSIAALYGADERFRGKVVMERHGFGRGEYKYFSYPLPPLVADLRTSLYPH
                     LAAIANRWNAALGIDIRYPAAHSAFLARCHAAGQTKPTPLMLRYGAGDYNCLHQDLYG
                     EHVFPLQATILLSDPDRDFTGGEFVLTEQRPRMQSRPEVVPLCQGDAVIFAVQHRPVQ
                     GSRGAYRVNMRHGVSRVRRGHRQTLGVIFHDAA"
     gene            3111..>3374
                     /locus_tag="VHT22_07305"
     CDS             3111..>3374
                     /locus_tag="VHT22_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEX3137399.1"
                     /translation="MSGDLFDLDGVEARQHQLAPGAVLLGGFAGAASAVLAADLQHVI
                     ARAPFRQMVTPGGLSMSVAMTNCGACGWVSDRTGYRYDPIDPDS"
BASE COUNT          533 a         1112 c         1171 g          558 t
ORIGIN      
        1 ccgacttgaa gcgccaggat gacgccgcgg gcaaggcgct gacctcactg cccgaggagc
       61 gcgagggcaa agtcgtctat gacttcttgc agtcgccatt gggcgccaag ctgctcgagc
      121 tgcaaaacga agcctgggga cggatcgtcg ccaatgtgtt taccggcgag atcgacgcct
      181 ggacgttggc gaacaaggct tcgctcgcga aggcggtgcg cgcggcagct cccggcactc
      241 cgccgccttc cgacaagacc tacctgggca aggtgacgat ggacgcgggc cgcggattca
      301 ccgtcacggt cgagcactat gcgaccttgc gccccgtggg gagctacgtg ctgaattttt
      361 cgccgaacga tttgcactgg accgacatcg ccgctgcggt acccggcatc gaacccggac
      421 agacgcgcgt gctctatcgc gatacctccg gtcacttggg cgaccggccg tgacgatgcg
      481 accggccgca cgcgcgctgc gaatgttgat gaccggcgcg ccgctcgtgc tgatgttcgc
      541 ctcgctgccc gcctgcggat gggaagacaa aagcggcgcc gacatgcctt ggctgggcgg
      601 cggcggccgc gcgcatctgt accaccagcg ttcttcgtgc atgggttgcc caaccggcta
      661 tcaggatgag gagcggtggg gcggctcggc ctggtccggg aagagcccat acgccaccgt
      721 tggaaaagcg atggagtttc ttgccgaaaa tgcgggcgcg cttcgcagcg ctcgatcgat
      781 cggcgccggt gccatcggcg gcgccggcgg caaagacggc atcatcgagc cgcgcgctct
      841 gccttaccgg atgacggtag tgatgatcga gccgaccgtg gtggtgatga atttcgatct
      901 cggcgcgatg atccatgaag gcgagccgca aacgggacag atcgtcggcg ccgcgtatat
      961 cgacggcgtg gtcggctacg gcacgcgcgt gccggcaacc gggtcgctgc tctggttctc
     1021 gcccgaggcc aaggcggcgc ctgcgcccgt cgcgaaccag agctctggca aaggcgagat
     1081 ccgggtcggc ggcgtgacct tgtcgctcga gcgcggcgcc aactactgga cggtgacgcg
     1141 gcgctgatcg ttttctgctc gggcttgcgt cggtcgaacc ggattcgaaa gcaaggcgcg
     1201 gagtgcgcag gacttgggcc ggaggttaaa tctcagccat cgataacgat gagctgaaag
     1261 gattcaacat gaagacgact tcgaaaagca ctgcacgggc cgccgcgacg atggcggacg
     1321 cacgttgggc ggcggtcatc gcgcgcgacc ctgccgccga cggcggcttt gtgtattcgg
     1381 tccgcacgac cggcgtctac tgccggcctt cgtgcggcgc ccgccaggcg cggccggaac
     1441 acgtggtgtt ccacctgacg gccgccgacg ccgagcgcgc gggtttccgg ccgtgcctgc
     1501 gctgccggcc cgaccagccg ccgctggccg agcagcacgc ggcgacggta gcgaggcttt
     1561 gccgtttcat cgaaagcgcc gagcaggcgc ccagtctgga aacgctggca gtagaggccg
     1621 ggttgagcgt ctatcacctg catcgcgtgt tcaaggcggt gaccggcttg acgccgaagg
     1681 cctatgccgc cgcacaccgc gcccggcgcg tgcgtgccga gctcgatcgc agcggcaccg
     1741 tgaccgaagc gatctatggg gcaggataca actccaatgg acgcttttac gcgcaatcca
     1801 acgacgtgct gggcatgacg ccttcgcgct atcgcgccgg cggcgccgat accaatatac
     1861 gattcgcgat aggtgagtgc tcgcttggcg cgatcctcgt ggccgccagc gaggtcggcg
     1921 tgtgcgcgat cctgatcggc gacgatcccg atgcgctggc gcgcgacctg caggatcgat
     1981 tcccgcgcgc cgagctgatc ggcggcgatg ccggtttcga ggagctggtc gcgaaagtgg
     2041 ttggcctggt cgaggcgccg ggccttggcc tcgacctgcc tttggacgtg cgaggcaccg
     2101 cgttccagca gcgggtctgg caggcgctgc gcgagatccc cgccggcaac acggtcagct
     2161 atgccgacgt cgcccagcgg atcggcgcgc cgaagtcggt gcgggctgtc gcgcaggcct
     2221 gcggtgccaa cgcactggcc gtggcgattc cctgccatcg ggtcgtacgc aacgacgggg
     2281 cgctggccgg ttatcgctgg ggcgtcgagc gcaagcgcgc gctgctcgag cgcgagtcgc
     2341 gccgatgaac gcgcgcgacc gcgccgtcgc gcacgttgcc gacatcgccg cggccgatgc
     2401 ccagacacgg ctggtctcgg ccgactgggg ggagatcgcc agcgatctcg atgctcgcgg
     2461 cagcgccgtc atcgagcgcg tgatcagccc ggcgcactcc cggtcgatcg ccgccctgta
     2521 cggggccgac gagcgctttc gcggcaaggt cgtcatggag cgtcacggct tcggccgcgg
     2581 cgagtacaag tacttcagct atccgctgcc gccactggta gccgacttgc gcacttcgct
     2641 ctacccgcac cttgccgcga tcgccaaccg ctggaacgct gcgctcggca tcgatatccg
     2701 gtatccggcc gcacattcgg cgttcctcgc gcgctgccac gcggcgggcc agaccaagcc
     2761 gacgccgctg atgcttcgct acggcgccgg cgactacaac tgcctgcatc aggacctcta
     2821 cggcgagcac gtgtttccgc tgcaggcgac gatcctgctg tcggatccgg atcgcgattt
     2881 caccggcggt gagttcgtgc ttaccgagca gcgaccgcgc atgcagtcgc ggccggaagt
     2941 cgtaccgcta tgtcagggcg acgccgtgat tttcgccgtc cagcaccgtc cggtgcaggg
     3001 ctcgcgcggc gcctatcgcg tcaatatgcg gcacggcgtc agccgcgtgc gtcgcggcca
     3061 tcgccaaacg ctgggcgtca tcttccacga tgccgcgtga cgacagcacc atgagcggcg
     3121 atctgttcga cctcgatggg gtcgaagcgc gacagcatca gctcgcgccg ggagcggtgt
     3181 tgttgggtgg gttcgccggc gcggcatcgg cggtgcttgc ggccgatctg caacacgtga
     3241 tcgcgcgagc gccgtttcgc cagatggtca cgccgggcgg actgtcgatg tcggtagcga
     3301 tgaccaattg cggcgcctgc ggctgggtca gcgatcgcac cggctatcgg tacgacccga
     3361 tcgatcccga cagc
//
LOCUS       DATANS010000495         1761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_194758, whole genome shotgun sequence.
ACCESSION   DATANS010000495 DATANS010000000
VERSION     DATANS010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194758"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VHT22_07310"
     CDS             complement(<1..224)
                     /locus_tag="VHT22_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX3137400.1"
                     /translation="MFRIPHLPLALIAGALGVLGFAPFTFPPILIAALALLIVLWRRA
                     ATRRQAALIGFVFGLGYFLTGVSWVYVSLH"
     gene            complement(256..1089)
                     /locus_tag="VHT22_07315"
     CDS             complement(256..1089)
                     /locus_tag="VHT22_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEX3137401.1"
                     /translation="MADSPAKPSWLERVGALLMREPEDRDQLVELLRSAYDRNLLDSD
                     ALSMIEGVLQVAEMQARDIMIPRAQMDVINISEPPDKFIPLVIETNHSRFPVIEHDKD
                     HVIGILLAKDLLRYYAGEEEFNVREMLRPAVFIPESKRLNVLLKDFRANRNHIAIVVD
                     EYGGVAGLVTIEDVLEQIVGDIEDEYDFDETEDDIIADKSGAWRVKAVTEIAKFNTTF
                     GTRFDDEDHDTIGGLVLKQFGRMPKRGDQLTVDNLIFKVLRADSRRLHLLLVEKKPGT
                     K"
     gene            complement(1079..1606)
                     /gene="ybeY"
                     /locus_tag="VHT22_07320"
     CDS             complement(1079..1606)
                     /gene="ybeY"
                     /locus_tag="VHT22_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX3137402.1"
                     /translation="MSGTPKRKPGRKPELALTVQYATRCAELPTPAKLRRWARSALRK
                     AARVTLRLIGEREARELNRIYRGRDYATNVLTFIYSKRSPLEGDIAICAPVVAREASR
                     QRTRREAHYAHLTVHGMLHLQGYDHARAPAAKRMENLETRILARLGFADPYASEAGGV
                     RRAAKEPTPYRKHGR"
     gene            complement(1566..>1761)
                     /locus_tag="VHT22_07325"
     CDS             complement(1566..>1761)
                     /locus_tag="VHT22_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX3137403.1"
                     /translation="GDVTQVDLAKGQASGLRDARSVLEGVRGIAVTEFQSDDVVRHPL
                     VQRIVNAYERHTKAQAGPEA"
BASE COUNT          290 a          560 c          573 g          338 t
ORIGIN      
        1 tcgtgcaggc ttacatagac ccagctgacc ccggtcagga aataaccgag gccgaatacg
       61 aatccgatca gcgccgcctg gcgccgcgtc gccgcccgcc gccacagcac gatcagcagc
      121 gcgagcgccg caatcaatat cggagggaag gtgaacgggg cgaacccgag cacgccgaga
      181 gccccggcaa tcaatgccag cggcaggtgg gggatgcgaa acagcgcgcc gtgggagact
      241 gggctcaagc cggccttatt tggtgccggg cttcttttcg accagcaaca aatgcagccg
      301 gcggctgtcg gcgcgcagca ccttgaaaat caggttgtcg acggtcagct gatcgccgcg
      361 cttgggcatg cggccgaatt gcttgagcac gagaccgccg atcgtatcgt gatcttcgtc
      421 gtcgaagcgc gtgccgaaag tcgtgttgaa cttggcgatt tcggtcaccg ccttgacgcg
      481 ccacgcgccg ctcttgtcgg cgatgatatc gtcctcggtc tcgtcgaaat cgtattcgtc
      541 ctcgatatcg ccgacgatct gctcgagcac gtcctcgatc gtcaccaggc cggcgacgcc
      601 gccgtactcg tcgaccacga tcgcaatgtg attgcggttc gcgcggaaat ccttgagcag
      661 cacgttgagc cgcttcgatt cgggtatgaa cacggcgggg cgcagcatct cgcgcacgtt
      721 aaattcctct tcgcctgcgt agtagcgcaa cagatctttg gcgagcagga tcccgatcac
      781 gtggtccttg tcgtgctcga tcaccgggaa ccgcgagtgg ttggtctcga tcaccagcgg
      841 aatgaatttg tcgggcggct cgctgatatt gatcacgtcc atttgggcgc gcgggatcat
      901 gatgtcgcgg gcctgcatct ccgccacttg cagcacgccc tcgatcatcg acagcgcgtc
      961 gctgtcgagc agattgcggt cataggccga gcgcagcaac tcgacgagct ggtcgcggtc
     1021 ctcgggttcg cgcatgagca gcgcgccgac gcgttcgagc cagctcggtt tggcgggact
     1081 atcggccatg tttgcgatag ggcgtaggct ctttcgccgc tcgcctcact cctcctgcct
     1141 cgctcgcgta agggtccgca aatcccaagc gtgcgaggat gcgcgtttcc aggttctcca
     1201 tgcgttttgc cgccggcgcg cgcgcgtgat cgtagccctg cagatgcagc atgccgtgca
     1261 cggtcaggtg cgcgtaatgc gcttcgcggc gcgtgcgttg gcggcttgct tcacgcgcga
     1321 ctaccggtgc gcagatcgcg atatcgccct cgagcgggct gcgtttcgaa tagatgaatg
     1381 tcagcacgtt cgtcgcgtag tcgcgcccgc gataaatgcg gttgagctcg cgcgcttcgc
     1441 gctcgccgat gagacgcagc gtgacgcgcg cagctttgcg cagcgccgag cgcgcccagc
     1501 gccgcagctt cgcgggcgtc ggcagttctg cgcagcgagt ggcatattgg accgtgaggg
     1561 cgagctcagg cttccggccc ggcttgcgct ttggtgtgcc gctcataagc attgacgatg
     1621 cgttgcacga gcgggtggcg caccacgtcg tcggactgga actcggtgac ggcgatgccg
     1681 cgcacgccct ccagcaccga gcgcgcgtcg cgcagccccg aggcctggcc tttggcgagg
     1741 tcgacctgcg tcacgtcgcc g
//
LOCUS       DATANS010000496         1813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_320944, whole genome shotgun sequence.
ACCESSION   DATANS010000496 DATANS010000000
VERSION     DATANS010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320944"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="VHT22_07330"
     CDS             <1..1074
                     /locus_tag="VHT22_07330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide chain length
                     determinant"
                     /protein_id="HEX3137404.1"
                     /translation="IDTRTAVKFVDEQIKQYEMTLKNTESRLKDFKLKYMGVAGQGNQ
                     DYFVKLAALSNSINAAKLDLRAAEESRDSYKRELAGEAPVLLPEGPAAPVAVPEIDAR
                     LATLKAQLDALLRQYTDEHPDVIGTQRVIQQLEEQRRQYIQSRAKASAKSGAPGEGTV
                     SRNPVFQQLRVSLADAEAEVASLQAKVNSYQAQYAQLKAEAQLVPQVEAEFAQINRDY
                     DIQKKTYETLLQRRQSAAIGEGVQDAGGTQFRVVDPPRVAPQPVPPTRIALLGIALAA
                     ALAAGLITSFVVSEVMPTFHDARSLREVTQRPILGMVTMLPSEALNRSRRRNALYFAG
                     GASGLVAAFAAVFAIALLFGRWA"
     gene            1085..>1813
                     /locus_tag="VHT22_07335"
     CDS             1085..>1813
                     /locus_tag="VHT22_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning ATPase"
                     /protein_id="HEX3137405.1"
                     /translation="MSLIEQAAKRLEELRRAGAEVPGASSSDGAEVASLDGVERLPTP
                     EAAIRGLAARGAVPEPVAPSPNQAPLSRDIRRSDGHEQNRIEIDLERLKARGFVTPDA
                     PKSQIADEFRVIKRPIIHNARRTAASAIRNGNLIMVTSALPGEGKTFTAVNLALSVAM
                     ELDTTVLLVDGDVAHPELPRVLGTPASPGLLELLTTNELDVSDAIVRTNVDNLSILPA
                     GAHHRRATELLASEQMAIVLHELGT"
BASE COUNT          347 a          600 c          578 g          288 t
ORIGIN      
        1 atcgatacgc gcactgcagt caaattcgtc gacgagcaga tcaagcaata cgaaatgacg
       61 ctgaagaata ccgaaagccg cctgaaggac ttcaaactga aatacatggg cgttgccggt
      121 cagggcaatc aggactattt cgtcaagttg gcggcgctca gcaattcgat caatgccgcc
      181 aagctcgacc ttcgcgcggc ggaagagtcg cgtgattcgt acaagcgcga gctcgccggc
      241 gaggcgccgg tcctgctgcc cgagggtccg gcggcgccgg ttgcggttcc agagatcgat
      301 gcgcgcctgg ccacgctgaa ggcgcaactc gacgctctgc tgcgacagta cacggatgaa
      361 catccggacg tgatcggcac ccaacgggtc atccaacagc tcgaggagca gcgccgccag
      421 tacatccagt cgcgcgccaa agcctccgcc aaatccggcg cgcccggcga aggcacagtg
      481 agtcgcaacc cggttttcca gcagttgcga gtctcgcttg ccgacgccga ggccgaagtc
      541 gcgtcgcttc aggcgaaagt caactcctac caggcgcagt acgcgcagct caaggccgaa
      601 gcgcaactcg tgccccaggt cgaagccgag ttcgctcaga tcaatcgcga ctacgatatc
      661 cagaagaaga cctacgagac cctgttgcag cgtcggcaat ccgcggcgat cggagagggg
      721 gtgcaggacg ccggcggcac gcaattccgc gtcgtcgatc cgcctcgcgt ggcgccgcag
      781 ccggtcccgc cgacgcggat cgcgttgctc ggcatcgcgc tcgcggccgc cctggcggcc
      841 gggctgatca cgagctttgt cgtcagcgag gtcatgccga cgtttcacga tgcgcgctcg
      901 ctgcgcgaag tcacgcagcg gccgatcctg ggcatggtaa cgatgttgcc aagcgaagca
      961 ctcaatcggt cgcgccggcg caacgcactc tatttcgccg gtggcgccag cggactggtc
     1021 gccgcgttcg ccgccgtctt tgcaatcgcc ttgctgttcg ggcgttgggc gtgaggggca
     1081 atcgatgagc ctgatcgagc aagcggccaa gcggctggaa gaattgcgcc gagccggggc
     1141 cgaagtgccc ggtgcgtctt cgtccgacgg cgcagaagtg gcttccctcg acggcgtcga
     1201 gcgtctgccg acgccggaag cggcgatccg cggcctagct gctcgcggcg ccgtgccgga
     1261 gccggttgcg ccttcgccca accaggcacc gctctcccgc gacatccggc gatccgatgg
     1321 ccacgaacag aaccggatcg agatcgacct ggagcggctg aaggccaggg gcttcgtcac
     1381 gcccgatgcg ccgaaatcgc aaatcgccga cgaatttcgc gtcatcaagc ggccgatcat
     1441 tcacaacgcc cgccgtaccg cggcatcggc catccgcaac ggcaatctga tcatggtcac
     1501 cagcgctttg ccgggggaag gcaagacatt cacggcggta aacctagcct tgagcgtggc
     1561 gatggaactc gatacgaccg ttcttttggt cgacggcgac gtcgcgcacc ccgaattgcc
     1621 aagggtgctc ggcacgcccg cctcgcccgg actgctcgag ctgctgacga ccaacgaact
     1681 cgacgtcagc gacgccatcg tgcgcactaa cgtcgacaac ctgtcgatcc tccccgccgg
     1741 ggcgcatcat cgtcgggcga cggagttgct tgccagcgag cagatggcga tcgtgctgca
     1801 cgagctcggg acg
//
LOCUS       DATANS010000497         2047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_278579, whole genome shotgun sequence.
ACCESSION   DATANS010000497 DATANS010000000
VERSION     DATANS010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278579"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..573)
                     /locus_tag="VHT22_07340"
     CDS             complement(28..573)
                     /locus_tag="VHT22_07340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3137406.1"
                     /translation="MAAADTRPAIFVRDARDADMHAVTKIYAAHVLHGLASFEEIPPS
                     LEEMQSRRASVLQLGLPYLVAEMDGRVVGYSYATFYRTRPAYRYTVEDSVYVEDGLNG
                     RGIGTLLLAELIARCERGPWRQMLAVIGDSANAGSIALHRRMGFTPAGNLTSVGFKFG
                     RWVDSVLMQRALGSGDATSPS"
     gene            complement(654..1043)
                     /locus_tag="VHT22_07345"
     CDS             complement(654..1043)
                     /locus_tag="VHT22_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEX3137407.1"
                     /translation="MKVETFTPANVMKPIGPYSHIAKAGSFIAISGTAGVDAATGDFA
                     GPDAYSQARQILKSLRSMLEGVDSDLDHVLHVNVFLKNAADFEEMNRAYREAFGTHLP
                     ARTVVAVAELPKKQALMTMNLTAVTKN"
     gene            complement(1075..1389)
                     /locus_tag="VHT22_07350"
     CDS             complement(1075..1389)
                     /locus_tag="VHT22_07350"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX3137408.1"
                     /translation="MLDDPHSHVMRIAEEVETEWEGRARRPWTRGSEGAIPAPSAPAR
                     NAIQAAVLALQQRQRELLLLHVDGLTYRQIASRNGLPEDVVLRELVSAYCALRRAVPD
                     SR"
     gene            complement(1540..>2047)
                     /locus_tag="VHT22_07355"
     CDS             complement(1540..>2047)
                     /locus_tag="VHT22_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX3137409.1"
                     /translation="IEIAVLLPSVYNELRRIALVNMARMPSGSTLQPTALVHEAWLRL
                     GGSEKSDWKGRAHFLAAAAEAMRHILIDRARQRSAVRHGGTQQRVDIDHIDIPIDTED
                     DEQLLAVNAALEKLAIDHPQAAELVKLRCFVGLEISEAARVLQIPRATAYRRWQFART
                     WLYQELHP"
BASE COUNT          357 a          624 c          688 g          378 t
ORIGIN      
        1 gcaataccag ggcggcgacg ccgagactca tgacggcgac gtcgcgtcgc cggagccgag
       61 cgcgcgctgc atcagcacgc tgtcgaccca gcggccgaac ttgaagccga ccgaggtcag
      121 gttgccggcg ggcgtgaagc ccatccgccg atgtaaggcg atggagccgg cgttggcgct
      181 gtcgccgatc accgcgagca tctggcgcca cgggccgcgc tcgcaacgtg cgatgagctc
      241 ggcgagcagt agcgtgccga tgccgcgtcc gttcagtccg tcttcgacgt agacggaatc
      301 ttccacggtg tagcgatagg caggccgcgt gcgatagaac gtcgcgtagc tgtagccgac
      361 gacgcgtccg tccatctcgg cgacgagata aggcaagccg agttgcaaca cgctcgcgcg
      421 ccgcgactgc atttcttcga gcgacggtgg aatctcttcg aacgatgcga ggccgtgcag
      481 gacgtgcgcg gcgtagattt tcgtgaccgc atgcatgtct gcgtcgcgcg catcgcgcac
      541 gaagattgcg gggcgggtat cggcggctgc cacgcgagca gtttcatcca acgcgcaacg
      601 ttcggcaatc gccgaacgct gtccaatttg gtgcaaagag gctcgagcgc tgctcagttt
      661 ttggtcacgg ccgtgagatt catcgtcatc agcgcttgct tcttcggcag ctcggcgacg
      721 gcgaccacgg tgcgcgccgg cagatgagtg ccgaacgcct cgcgatacgc gcgattcatc
      781 tcttcgaagt ccgcagcgtt cttgaggaac acgttcacgt gcaggacgtg gtcgagatcc
      841 gagtcgacgc cttcgagcat cgagcggagg gacttcagga tctgtcgcgc ctggctatac
      901 gcgtccggcc cggcgaagtc gccggtcgcg gcatcgacgc cggcagtgcc ggagatcgcg
      961 atgaatgagc cggctttggc gatgtgactg tacgggccga tcggcttcat cacattcgcg
     1021 ggcgtaaagg tttcgacttt catgagcgca ctccagcagg gcgagtgcgg aattctagcg
     1081 cgagtccggt actgcccggc gcagtgcgca gtacgcgctg accagctcac gcaagaccac
     1141 gtcttccggc aagccgttgc gactcgcgat ctgccgatag gtgagcccat cgacatgcag
     1201 cagcaacaac tcgcgctgac gctgttgaag cgcgagcacg gcggcctgga tcgcgttgcg
     1261 agcgggtgca gaaggagcgg ggattgcacc ttcgctgccg cgcgtccagg gccgccgtgc
     1321 gcgcccctcc cattcggtct cgacctcctc ggcgatgcgc atgacatggc tgtgcggatc
     1381 gtcgagcaag ctcgcgtggt tcgcggcaca cagtcggcgg gagacctcct gtgccagagc
     1441 gtctacgttt gccatcgtca gaaatcgagt ccgctgagac ctgttgtctt gttgcgagtc
     1501 gtcttcgtca atgaatacga aattcgcaag cgcagcgtct cagggatgca gctcctggta
     1561 gagccaggtg cgcgcgaact gccagcgtcg gtacgcagtc gctcggggaa tctgcagcac
     1621 tcgggctgct tccgagatct ccaggccgac gaagcaacgc agcttcacga gctcggcggc
     1681 ctgcggatga tcgatcgcca gtttctccag cgcagcgttg acggccagga gctgctcgtc
     1741 gtcttccgtg tcgatgggaa tgtcgatgtg atcgatgtcg acgcgttgct gcgtgccgcc
     1801 gtggcgcacg gcactgcgct gcctcgcgcg atcgatgagg atgtgacgca tcgcttcggc
     1861 cgccgccgcg aggaaatgcg cacggccttt ccagtcggat ttctcggagc cgccgaggcg
     1921 cagccatgct tcgtgaacga gcgccgtggg ttgcagcgtg ctgcctgaag gcatgcgcgc
     1981 catgttgacg agagcaatgc ggcgcagttc attgtagacg gaaggcagga gtacggcgat
     2041 ttcgatg
//
LOCUS       DATANS010000498         1810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_488065, whole genome shotgun sequence.
ACCESSION   DATANS010000498 DATANS010000000
VERSION     DATANS010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488065"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VHT22_07360"
     CDS             <1..396
                     /locus_tag="VHT22_07360"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HEX3137410.1"
                     /translation="LTYQQNKVYAWSEEGTSSTAMSFGHQKRDSVVSSLGWQASGTFG
                     ALRPFGRVTWDKEYDNGERTVRAGLVSTGGIGFGLPALRIDDNYVLFEIGASADLGNR
                     LIGFASINATASKDDGNFQAVTVGVRLPL"
     gene            complement(439..1266)
                     /locus_tag="VHT22_07365"
     CDS             complement(439..1266)
                     /locus_tag="VHT22_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEX3137411.1"
                     /translation="MSDDRARRDALGVVHPVARADARIVSLVPSLTELLFTLGLGDQV
                     VGRTGFCIHPRDALRRVPKVGGTKDVALDKVLALKPTHLIVNIDENRRETVDALAAQV
                     PHVIVTHPLVPDDNLQLYRMFATIFGRPAEAEALAARYVAARDTLLDAVSTLPHQNVL
                     YLIWRKPWYTVARSTYVSAMLALACWRTVPAISAPRYPTLDDDAPAWREAERILLSTE
                     PYAFREKDASALALMLASRGQPTPVTLIDGEMTSWYGARAVAGLEYLAQMRRNLAGR"
     gene            complement(1277..>1810)
                     /locus_tag="VHT22_07370"
     CDS             complement(1277..>1810)
                     /locus_tag="VHT22_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3137412.1"
                     /translation="DVVIANAGISVGTSTQYAADIASFRAVLETNVLGVVHTFQPFVA
                     GMMAARHGTLVGIASVAGFRGLPGAGAYSASKAAAINYLESLRVELVGSGVEVVTICP
                     GFIATPMTANNPYRMPFLLAPEKAARLIAGAIARGRRFYVLPWQMAWLGRTLRVLPRP
                     VFDRVLAKRPRKPRASA"
BASE COUNT          328 a          597 c          601 g          284 t
ORIGIN      
        1 ctaacctatc agcagaacaa ggtctatgcc tggtcggagg aaggcaccag cagcacggcg
       61 atgtcgttcg gtcaccaaaa gcgcgactcg gtggtctcca gcctgggctg gcaggcaagc
      121 ggcacctttg gagcgctgcg gccgttcggg cgagtcacct gggacaagga atacgacaac
      181 ggcgagcgca cagtacgcgc cggactcgta tcgaccggcg gcatcggctt cggcctgccg
      241 gcgcttcgta tcgacgacaa ctacgtgctg ttcgagatcg gcgccagcgc cgatttgggc
      301 aacaggctca tcggcttcgc cagcatcaat gcgacggcat ccaaggacga cgggaatttc
      361 caggcggtga cggtgggcgt gcgcctgcct ctttgagctg accgcgcacg aaaaaggccg
      421 cctcatgcgg cctttttctt aacgccccgc caggttccgg cgcatctgcg cgaggtactc
      481 cagcccggcg acggcgcgag cgccgtacca cgaagtcatc tccccgtcga tcagggtgac
      541 cggcgtcggc tggccgcggc ttgcaagcat caacgcgagc gcgcttgcgt ctttctcgcg
      601 gaatgcataa ggctcggtcg agagcaggat tcgttcggcc tcgcgccacg ccggcgcatc
      661 gtcgtcaagc gtcggatagc gcggcgcaga aatcgcaggc accgttcgcc agcacgccag
      721 agccagcatc gccgacacat aggtcgatcg cgccaccgtg taccacggct tgcgccagat
      781 caggtaaagc acgttctggt gtggaagagt gctgactgcg tcgagcaagg tgtccctggc
      841 cgccacgtag cgcgccgcca aggcttcggc ctccgcgggc cggccgaaga tcgtcgcgaa
      901 catccggtac agctgcaagt tgtcgtccgg caccagcgga tgagtgacga tcacatgcgg
      961 gacttgcgcg gccagcgcat cgacggtctc gcggcgattt tcgtcgatat tgacgataag
     1021 gtgcgtcggc ttgagggcga gaaccttgtc cagggcgaca tccttggtgc cgccgacctt
     1081 gggcacgcgc cgcaacgcgt cgcgcgggtg gatgcaaaaa ccggttcgac ccacgacctg
     1141 gtcgcctagg ccgagcgtga acagcaactc ggtcaggctc ggcaccaggc tgacgatgcg
     1201 cgcgtcagcc cgggcgaccg ggtgcacgac gccgagggcg tcgcgccgcg ctctgtcgtc
     1261 cgacatcggt tcggacctag gccgacgcgc gcggcttgcg cggccttttc gccaggaccc
     1321 tgtcgaaaac cgggcgcggc agcacgcgca gcgtgcggcc cagccatgcc atctgccatg
     1381 gcagcacgta aaagcggcgc ccgcgggcga tcgcaccggc gatcagccgc gcggcttttt
     1441 ccggcgcgag caggaaaggc atgcgatacg gattgttcgc ggtcatcggc gtggcgatga
     1501 agccgggaca gatcgtgacg acttcgaccc cgctgccgac gagctcgacg cgcaggcttt
     1561 cgagatagtt gattgccgcg gccttcgaag ccgaatacgc ccccgcgccc ggcagcccgc
     1621 gaaagcccgc cacgctcgcg attccgacca gcgtcccgtg gcgggccgcc atcatgcctg
     1681 caacaaacgg ctggaacgtg tgcacgacgc cgagcacatt ggtctcgagc accgcgcgga
     1741 acgacgcaat atcggccgca tactgggtcg aggtgccgac cgagatgccg gcgttggcga
     1801 taacgacgtc
//
LOCUS       DATANS010000499         1773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_361491, whole genome shotgun sequence.
ACCESSION   DATANS010000499 DATANS010000000
VERSION     DATANS010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361491"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1349
                     /locus_tag="VHT22_07375"
     CDS             <1..1349
                     /locus_tag="VHT22_07375"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX3137413.1"
                     /translation="TGLERRLVPWRATARHRLLEVIIEEFVRIVLGRVEKLWGERRGS
                     GTLVAGTNRRRCPMPQSNDLSRSLTALDQDSTLIAVVEMSQSSWLVGAIIPGVERRPA
                     KKLEASEGMLLALLHRWRDEAAKAGCTIDRITVAFEAGRDGFWLARWLRQRGIEAHVI
                     HATSIAVSREHRRAKTDRLDAALLQRAFLGWLRGERGHCTMVAIASLAEEDAKRPSRE
                     REALVGERTRIGNRMKSTLARLGIRGIRPGLRKAAERLEQLRTPEGAPLPPNTLAELR
                     RDLARLRLINDQIGEIEAARLERLRQQPDEARHPMILLLARVVGIGIETADMLVQEVL
                     SRKLRDRRAVARYGGLTGSPEESGARRREKGLARAGNARVRRGMLQLAWRFLMFQKDS
                     ALAQWYRARTADARGATRKTMIVALARKLLVALWKLVTTGELPNGVVLRPAIQAVL"
     gene            complement(1658..1773)
                     /locus_tag="VHT22_07380"
                     /pseudo
     CDS             complement(1658..1773)
                     /locus_tag="VHT22_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          297 a          564 c          634 g          278 t
ORIGIN      
        1 agaccggact tgagcgccgc cttgtcccgt ggcgtgcgac ggcgcgccat cgcctgcttg
       61 aggtcattat tgaggaattc gtccggattg tgctcgggcg cgtagagaag ctatggggcg
      121 agcggcgggg atccggcacc ctcgtcgctg gaaccaaccg acggaggtgc cccatgccgc
      181 agtcgaatga cctgagccga tcgctcactg ctctggacca ggatagcacg ctgattgccg
      241 tggttgagat gagccagtcg agctggctgg ttggcgcgat cattccgggc gtcgagcggc
      301 gtccggccaa gaagctggag gcgagcgagg ggatgctgct ggcgctgctg catcgctggc
      361 gcgacgaggc cgcgaaggca ggatgcacga ttgaccgcat cacggtggcc tttgaggccg
      421 gccgcgacgg tttttggctg gcgagatggc tgcgacagcg cggcattgag gcgcatgtga
      481 tccacgccac cagcatcgcg gtctcgcgcg agcatcgccg ggcgaagacc gaccgtttgg
      541 acgccgcgct gctgcaacga gcgtttctcg gctggctgcg cggcgagcgc gggcactgca
      601 cgatggtggc gattgccagc cttgcggagg aggacgccaa acggccgagc cgtgaacggg
      661 aggctttggt cggcgagcgc acgcggatcg gcaaccggat gaagagcacg ctggcccggc
      721 tgggtattcg cggcatcagg ccgggtctgc gcaaggcagc cgagcggctc gaacaattgc
      781 gcacgcccga gggcgccccg ctgccgccca acacgctggc cgagctgcgt cgcgatctcg
      841 cccggctgcg cttgatcaac gatcaaatcg gcgagattga agccgcccgc ctcgaacggc
      901 tgcggcagca acccgatgag gctcgccatc cgatgatcct gttgctggct cgcgtcgtcg
      961 gcattggcat cgagacggcc gatatgctgg tccaggaagt actctcgcga aagctgcgcg
     1021 accgccgcgc cgtggcccgt tatggcggac tgaccggatc gcccgaggaa agcggcgccc
     1081 gccggcgaga aaagggcttg gcccgggcgg gcaatgcccg ggtgcgccgc ggcatgctgc
     1141 agctggcctg gcgcttcctg atgttccaga aggacagcgc gctggcgcaa tggtatcggg
     1201 cacgcaccgc tgacgcgcgc ggcgccaccc gcaagacgat gattgtggcg ctggcccgca
     1261 agctgctcgt cgccttgtgg aaactcgtga cgaccggcga gctgccgaac ggcgtggtgc
     1321 tgcgccccgc gatccaagcg gtgctctgac ggtaaccggt tatcgaacga gattgggcgc
     1381 gccgtcgaca atccgtctcg gcgctgctca tgacgatccg aggcggcggt gacccgaggc
     1441 aggcatggct gcgacgccgt cctagagaat gggcctgccg ccccggagct gcgccaccga
     1501 tgcgcatcac tgcatcatgg tcgaggtccg cacggacctg ccgaatacaa gggtgtagcg
     1561 cgctcttgcg cgcgccacgg cgagctcccc tcggatcctg cgatgaggct tgacatgcaa
     1621 atgccccata caagcctgcc ccggcgcagg ccggggacgg caggtagaag agttcgatct
     1681 gatcggcatg gtcctgcacc caggcggtga cccggtgcgc ccgatggacc ggcagattgt
     1741 cgacgatcac gaacagcttg cgcgcggcct cgc
//
LOCUS       DATANS010000500         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_488165, whole genome shotgun sequence.
ACCESSION   DATANS010000500 DATANS010000000
VERSION     DATANS010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488165"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VHT22_07385"
     CDS             complement(<1..560)
                     /locus_tag="VHT22_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX3137414.1"
                     /translation="MADHEFGFETLCLHAGHIPDAATGARALPIYQTTSFVFDSADHA
                     ASLFNLQTFGNVYSRISNPTVAALEERVAALEGGRAGLAAATGMAAQMLALLTLAQNG
                     DHIVAARTLYGGTYSQFAVTFAQFGIDTTFVDADDPEAFRQAIRPNTKCIYAETIGNP
                     QLNVLDIAAVAGIAHEAQVPLVIDNTL"
     gene            complement(646..1113)
                     /locus_tag="VHT22_07390"
     CDS             complement(646..1113)
                     /locus_tag="VHT22_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX3137415.1"
                     /translation="MALVDDVTGLRRILLQSRTIAVIGLSVNWYRPSFFAAKYMQDHG
                     YRIVPVNPTCAEVLGQKCYPTLREIPYKVDIVDCFRKPGEIPPIARDAVAIGAKVLWM
                     QLGIRNDQAAKIALDAGLDVVMNRCVKIEHARILGGLNWAGVNTGIVSARRPG"
BASE COUNT          261 a          458 c          515 g          268 t
ORIGIN      
        1 agcgtgttgt cgatcaccag cgggacttgc gcctcgtgcg caatgccggc gaccgccgcg
       61 atgtcgagga cattgagctg cggattgccg atcgtctcgg catagatgca tttggtgttc
      121 ggccggatcg cttgacggaa cgcctccggg tcgtcggcgt cgacgaaagt cgtgtcgatg
      181 ccgaattgcg caaacgtcac cgcgaactgc gaataggtgc cgccgtagag cgtgcgcgcg
      241 gcgacgatgt ggtcgccgtt ctgcgccagc gtcagcaggg cgagcatctg agcggccatg
      301 ccggtcgccg cggccaggcc ggcgcggccg ccttcgagtg cggcgacccg ctcctccagc
      361 gcggcgactg tcggattcga gatgcgcgaa tagacgttgc cgaatgtctg caggttgaac
      421 aggctcgctg cgtgatcggc gctgtcgaag acgaaggacg tcgtctggta gatcggcaac
      481 gcccgcgcgc cggtcgcggc atccggaata tgtccggcat gcaggcaaag cgtttcaaag
      541 ccgaattcgt gatcagccat cggaaccggt caaggcatgg cgctcgcgag gcgatgcgca
      601 aagcgcgcgc cgcacatcgt ggtcataccg ggtcgccgcg gcttctcatc ccggccgcct
      661 ggccgagacg atgccggtgt tgacgccggc ccaattgagt ccgccgagaa tgcgggcgtg
      721 ctcgattttc acgcagcggt tcatcaccac atcgagcccg gcgtcgagtg cgatttttgc
      781 cgcctggtcg ttgcggatgc caagctgcat ccacagcacc ttggcgccga tagcgaccgc
      841 gtcgcgggcg atcggcggaa tctctccggg tttacgaaag cagtcgacga tgtcgacctt
      901 gtatggaatc tcacgcagcg tcggatagca tttctggccg agcacttcgg cgcaggtcgg
      961 attgaccgga acgatgcggt acccgtgatc ctgcatgtat ttggccgcaa aaaaactcgg
     1021 gcggtaccag ttgacggaca ggccgatcac ggcgatggta cgcgattgca gcaggatgcg
     1081 gcgcagtccg gtgacgtcgt cgacgagcgc catccgagcc ttacctcgcg cgccccgcgc
     1141 tcggcgccgg gtcgctacta ccggtgcacg cgctggagtt gccgacgtgg ggtcggtaga
     1201 atccgggcgg catggcgatt ggcgggcggg tgtcgcggtg ggttgggcgc cgcaagtcgg
     1261 catctgcggc gaaacaggga cattttaatg catatccgac cctgtcccgc tgcgccggtc
     1321 gattgcgctg taagctggat gccgccagcg tcgaccaacg tgcgcggaaa cctttggtgc
     1381 ggaaaagccg tccaaacgcg cgacagcatg gagatctcac gatgaaacga aggcacttat
     1441 tggcatcgct cgccgcatgg ccgctggccg cggccggctt cggcaaggca atggccgccg
     1501 gc
//
LOCUS       DATANS010000501         7542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_820544, whole genome shotgun sequence.
ACCESSION   DATANS010000501 DATANS010000000
VERSION     DATANS010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820544"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VHT22_07395"
     CDS             <1..844
                     /locus_tag="VHT22_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3137416.1"
                     /translation="EVNAEISDAPGAPPLTAGGGEVRFEHVDFHYEANRQILFDVSFV
                     IPAGRTIAVVGPSGSGKSTLARLLYRFYDVSSGRITIDGQDLRSVQQTSVRAAIGIVP
                     QDTVLFNDSIEYNIAYGNPGATHEQIVIAARLAQIHDFVSTLPEGYRTPVGERGLKLS
                     GGEKQRVAIARAILKDPRILIFDEATSALDSKSEKLIQAELEEISAGCTTLTVAHRLS
                     TIVGADQILVLEHGRITERGTHAELLAAGGSYARMWALQQQRREGAEDGSPDPRNPSA
                     QVIA"
     gene            922..1893
                     /gene="pbpG"
                     /locus_tag="VHT22_07400"
     CDS             922..1893
                     /gene="pbpG"
                     /locus_tag="VHT22_07400"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF008668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HEX3137417.1"
                     /translation="MNSRLLIVLIGLFLVATGAGAAEDLKPIGSTLLIDASKLKLASA
                     NALIFDAQTGQPIYSKDADAVTAIASVTKLMTAMVMLDAQQPMDEALTIDIGDLDMLK
                     ASSSRLRLGSTMTRREMLQLALMSSENRAASCLARNYPGGTQAFVEAMNRKAASLGML
                     QTHFNDATGLSSENVATARDLARMVQAAAAYPLIREFTTTASHSVEIGPSGRVLGFNN
                     TNSLVRGGQWDITLSKTGFIREAGKCLVMLANIVNRPVVIVLLDSYGKLSRIADAQRV
                     KYWLETGESLALPTASHASAKAKRATHRVRGSFAAVSTADKAKARHL"
     gene            complement(2000..3682)
                     /gene="recN"
                     /locus_tag="VHT22_07405"
     CDS             complement(2000..3682)
                     /gene="recN"
                     /locus_tag="VHT22_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX3137418.1"
                     /translation="MLRLLSIRNFAVVEALEVEFRPGFTVLTGETGAGKSILLDALSL
                     LLGDRFETRQLRASTDRAEIAAEFDGDETPDLRAWLAAQDLADEGPLLLRRVLDAQGR
                     SRAYINGRPATLAQLADIGERLIDLHGQHAHQSLGRPEAQRQLLDSFGSVEPLAREVA
                     AAWRAWREAIERRDRGGREAEARQAERDALAARNAELAALGATADEWQTLSQAQSRLA
                     HAATLLETATTAEAEFTEGDAALGARLAVLAHRLRQAAQHDQALSDIVQLADEAKIRV
                     DEAGRSLRAYRERLDLDPGELSRVETRLAAFHDLARKHRVRPEALPALADETARRLAE
                     LVADSDAGALERAADQARQSFDALARELSRKRKAAALSLGKKVTAMMKELAMAGGRCE
                     ITLTTLAEPASFGAETVEFRVATHPKQPLGPLARVASGGELSRLALAIQVVLAEVGRV
                     PTLIFDEVDVGIGGAVAVTVGRMLRELGLRRQVLCVTHLPQVAACADEHYRVTKIGKG
                     DAVASELARLSAGARVEELARMLGGHEITAKTRAHAGELLAQQREATTRRAR"
     gene            complement(3810..4706)
                     /locus_tag="VHT22_07410"
     CDS             complement(3810..4706)
                     /locus_tag="VHT22_07410"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEX3137419.1"
                     /translation="MGGPAQRFATVALIGRYASAGIAEPLKRLAAFLADRGHDVILDA
                     ETAQCTPLPGYRTAPAEELGRNAQLAIAVGGDGTMLSIARRLAPFDVPLIGINQGRLG
                     FLTDIALADMESALSAMLDGRYVEETRTLLDATLTLATGGSERTLALNDVVVGRGAMG
                     GMIDCAVTIDGKFVYAMRADGLIVATPTGSTAYALSAQGPIVDPIVAALLLVPVAPHA
                     LTNRPIAVRDSAVIEITLLRGKDASLHCDGQSHFALVEGDRVVVRRAAHRLRLLHPQD
                     HDHFAMLRQKLHWSETPERLRP"
     gene            4801..5829
                     /gene="hrcA"
                     /locus_tag="VHT22_07415"
     CDS             4801..5829
                     /gene="hrcA"
                     /locus_tag="VHT22_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX3137420.1"
                     /translation="MVDPRSQHLLKTLIERYITDGQPVGSRALSRQSGLELSPATIRN
                     VMADLEEMGFIASPHTSAGRIPTAKGYRFFVDTLMVVKPLEQIEIRRLEEELHADHPQ
                     QIVNAAAVMLSQLTQFAGVVMTPKRREATFRHLEFLRLSERRVLLIIVTAENDVQNRI
                     LHTNRPYTQAQLIEATNFFNHNFAGSPLGAIRGRLADELRALGDDIAGLMRAAVDVGE
                     GALSRDESLVVSGERNLLNAGDFSSNMQRLRLLFDLFEQKTSLLHLLDASQQAEGVQI
                     YIGGESGLLPLDECSVVTAPYEAGGEVIGTLGVIGPTRMAYERVVPIVDVTAKLLSSA
                     LSQQIADD"
     gene            5929..7047
                     /gene="hemH"
                     /locus_tag="VHT22_07420"
     CDS             5929..7047
                     /gene="hemH"
                     /locus_tag="VHT22_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX3137421.1"
                     /translation="MLLMSFLPEPAFAHDRPRRIGVLLVNLGTPDAPTIPAVRAYLAE
                     FLSDERVIEIPRAAWLPILHGVILRVRPAKSARKYATIWGKDGSPLLVHSVKQKTLLQ
                     GYLGQRLKEMGLPSDHAQIELGMRYGRPTIDSAMARLKAGGCDRVLVLPLYPQYAAST
                     TASALDAVFATASRMRAVPALRVVAPYHDDPGYIKAVAQSINDYWVKNGRPNKLLLSF
                     HGVPRRSLDLGDPYHCHCHKTARLVASELGLTSEQYAVSFQSRFGRARWLEPYTLPTL
                     LALAKEGMRRVDIACPGFVSDCLETLEELGHDAQAAFLGAGGQELHCIPCLNERPGWI
                     AALADLAMRNLQGWLELPADAAAREMSQARAKALGADR"
     gene            7192..>7542
                     /gene="grpE"
                     /locus_tag="VHT22_07425"
     CDS             7192..>7542
                     /gene="grpE"
                     /locus_tag="VHT22_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX3137422.1"
                     /translation="MGENPDHNSQVSGEVEAGVPIPTDAPAGGPAARPGDPAPDLATL
                     LKQAEDEASRLKDAWLRAMAETDNVRKLAQNDVTKAHKYAIERFAQELLVVKDALEQT
                     LAAPAASIEALRDGV"
BASE COUNT         1273 a         2499 c         2532 g         1238 t
ORIGIN      
        1 cgaggttaac gccgaaatca gcgacgcgcc cggcgcgccg cctctcacgg ccggcggcgg
       61 cgaggtccgc ttcgagcacg tcgatttcca ctacgaggcc aaccggcaga tcctgttcga
      121 cgtgtcgttc gtgattcctg cgggccgcac gatcgccgtc gtcgggccga gcggctcggg
      181 caaatcgacg ttggcccggc ttttgtaccg gttctacgac gtcagcagcg gccgcatcac
      241 catcgacgga caggacttgc gctcggtgca gcagaccagt gtgcgcgcgg cgatcggcat
      301 cgtgccgcag gacacggtgt tgttcaacga ttcgatcgaa tacaacatcg cctacggcaa
      361 cccgggcgcg acgcacgagc agatcgtcat cgcggccagg ctcgcgcaga ttcacgattt
      421 tgtctcgaca ctgcccgaag gctatcgcac accggtcggc gagcggggcc tgaagctttc
      481 gggcggcgaa aagcagcgtg tggcgattgc gcgcgcgatc ctcaaggatc cgcgcattct
      541 gatcttcgac gaagcgacct cggcgctcga ttccaagtcg gaaaagctga tccaggcgga
      601 gctcgaagag atctcggcag gctgcacgac cctgaccgtc gcccaccgcc tgtcgacgat
      661 cgttggcgcc gatcagattc tggtactcga gcatgggcgt atcaccgagc gcggcaccca
      721 cgccgagttg ctggccgccg gcggctcgta tgcccgcatg tgggcgctcc agcagcagcg
      781 ccgcgaaggt gccgaagacg gcagccccga tccgcggaac ccgtcggccc aggtcatcgc
      841 ctgagggggt gggcgcgtta agccgttggc atagcgccaa agctgtgcta taaacttgcg
      901 tccctgctga gaactacctt aatgaactca cggttactta ttgttttaat tggtttattt
      961 ctggttgcta caggtgccgg cgccgccgag gacctgaagc cgatcggatc gacgctgctg
     1021 atcgacgcca gcaagctcaa gcttgcgtcg gccaacgcgt tgattttcga cgcccagacc
     1081 gggcagccga tctattccaa ggacgccgat gcggtaacgg cgatcgcctc ggtcaccaag
     1141 ctgatgactg cgatggtcat gctcgacgcc cagcaaccga tggacgaggc gctgacgatc
     1201 gacatcggcg atctggacat gctcaaggcg agcagctcga ggctgcggct gggcagcacg
     1261 atgacgcggc gggaaatgct gcagctggcg ctgatgtcgt cggaaaaccg cgcggcttcc
     1321 tgtctcgcac gcaactatcc tggcggaacg caagcttttg tcgaggcgat gaaccgcaag
     1381 gcggcgagcc tcggcatgct gcagacgcat ttcaatgacg ccaccggact gtcgagcgaa
     1441 aacgtcgcga ccgcgcgcga tctggcgcgc atggtacagg cggctgccgc ctatccgctg
     1501 atccgcgaat tcacgacgac cgccagccac tctgtcgaga tcggcccgag tggccgcgtg
     1561 ctgggtttca acaataccaa ttcgctggtg cgcggcgggc agtgggacat cacgctcagc
     1621 aagaccgggt ttattcggga agcggggaaa tgcctggtga tgcttgccaa tatcgtcaat
     1681 cggccggtcg tcattgtcct gctggactcg tacggcaagc tttcgcgcat cgccgatgcg
     1741 cagcgggtca agtattggct cgaaaccggc gaatcgctgg cgctgccgac cgcttcgcat
     1801 gcatcggcga aggccaagcg cgcgacccat cgggtgcgcg gcagcttcgc cgccgtgtcg
     1861 accgcggaca aggccaaggc gcgccacctg tagtcggttc gaacgtccaa tgacggccgc
     1921 gaaagcggcc gtttttcatt gctcgacggc cccgactggc ggcctcattg cgcgggcgcc
     1981 gcccgggcgg tcgccaagac tagcgcgcgc gtcgcgtcgt cgcctcgcgc tgctgcgcca
     2041 gcaattcgcc ggcgtgtgcc cgcgtcttgg cggtgatttc gtggccgccg agcattcggg
     2101 caagctcctc gacccgcgcg ccagcggaaa gtcgcgccag ctcggacgcg accgcgtcgc
     2161 cctttccgat cttggtgacg cggtagtgct cgtccgcgca tgccgccacc tgcggcagat
     2221 gggtgacgca gagcacctgc cgccttagcc cgagctcgcg cagcattcgg ccgacagtca
     2281 ccgccacggc gccgccgatg ccgacgtcga cctcgtcgaa gatcagcgtc ggaacacgac
     2341 cgacttcggc cagcaccacc tggatcgcca gcgcgagtcg cgacagctca ccgcccgacg
     2401 cgactcgcgc caacgggccg agcggctgtt tcggatgggt cgcgacacgg aattcgacgg
     2461 tttcggcgcc gaagctcgcc ggctcggcca gtgtcgtcaa tgtgatctcg cagcggccgc
     2521 cggccatcgc cagctccttc atcatcgcag tgaccttctt gcccagcgac agcgccgccg
     2581 ctttgcgttt gcgggagagc tcgcgtgcca gcgcatcgaa tgattgcctg gcttgatcgg
     2641 cggcgcgctc cagtgcgccg gcgtcggagt ccgcgacgag ctcggcgagg cggcgcgccg
     2701 tttcgtcggc cagcgccggc agtgcctcgg gacgcacgcg gtgcttgcgc gcgagatcgt
     2761 ggaacgccgc aagccgtgtt tcgacgcgcg agagctcgcc cggatcgagg tcgaggcgct
     2821 cgcgataggc gcggaggctc cggcccgctt cgtcgacgcg aatcttggcc tcgtcggcaa
     2881 gttgaacgat gtcggaaagt gcttgatcat gttgtgcggc ctgccgcaga cgatgcgcga
     2941 ggacggcgag ccgcgcgccg agggcggcgt cgccttcggt gaattcggcc tccgcagtcg
     3001 tcgccgtctc gagcaaggtc gccgcatgcg cgagccggga ttgagcctgc gacagcgtct
     3061 gccactcgtc ggccgtcgcg ccgagggcgg cgagctccgc attgcgcgcg gcaagcgcat
     3121 cgcgctcggc ttgacgcgcc tcggcctccc ggccgccgcg gtcgcggcgc tcgatcgctt
     3181 cgcgccaggc gcgccaggca gcagcgactt cgcgcgccaa gggctcgaca ctgccgaaag
     3241 aatcgagcag ctggcgctgc gcttcgggcc ggcccagcga ctggtgcgcg tgctggccgt
     3301 gcaggtcgat caggcgttcg ccgatgtccg ccagctgagc cagcgtcgcc ggccgtccgt
     3361 tgatgtaagc acggcttcgt ccctgcgcat cgagcacgcg gcgcagcaac agcggtcctt
     3421 cgtcggcgag atcctgcgct gcgagccacg cgcgcaagtc cggtgtctcg tcgccgtcga
     3481 actcggcggc gatttcggcg cggtcggtgc tcgcacgcaa ctgccgcgtc tcgaagcggt
     3541 cgccgagcag cagcgacaag gcatcgagca ggatcgattt cccggcgccg gtttcgccgg
     3601 tcaacacggt gaagccgggc cggaactcga cctcgagcgc ctcgacgacg gcgaaattac
     3661 gaatggaaag caggcgaagc atcgattcga ttttccggcg caaagcgcgc gcccccgatt
     3721 atcgccggaa gcaagcctcg ccgcgctccg acctaagcct taggcgactt aaggtaactg
     3781 aggcagccgg gggaatgcgc ccttttcgcc taaggacgaa gccgctccgg cgtctcgctc
     3841 cagtgcagct tctggcgaag catcgcgaaa tgatcgtgat cctgcggatg cagcaggcgg
     3901 agtcgatgcg cggcgcgccg gacgacgacg cgatcaccct cgaccagcgc gaaatgcgac
     3961 tggccgtcgc aatgcaggct cgcatccttg ccgcgcagca gcgttatctc gatcaccgcg
     4021 gagtcgcgca ccgcgatcgg gcggttggtg agcgcgtgtg gcgcgaccgg gaccagcaac
     4081 agggcggcga cgatcggatc gacgatcgga ccctgtgccg acagcgcgta tgcggtcgat
     4141 ccggtgggcg tggcgacgat cagtccgtcg gcgcgcatcg catagacgaa cttgccgtcg
     4201 atcgtcaccg cgcagtcgat cattccaccc atcgcgccgc gaccgacgac gacatcgttc
     4261 agcgccagcg tgcgctcgct gccaccggtg gccagcgtca gcgtcgcgtc gagcagcgtg
     4321 cgtgtctctt ccacgtaacg cccgtcgagc atcgcggaca gcgcgctttc catatcggcg
     4381 agcgcgatgt cggtcaggaa tccgaggcgg ccctgattga tgccgatcag cggcacatcg
     4441 aacggcgcga gccggcgagc gatcgacagc atcgtgccgt cgccaccgac agcgatcgca
     4501 agttgggcgt tgcgcccgag ctcttccgcc ggcgcggtgc ggtagcctgg caacggcgtg
     4561 cactgcgcgg tctccgcatc gaggatgaca tcgtgaccgc ggtccgccag aaacgccgcc
     4621 aggcgcttga gcggctcggc gatgccggcg ctcgcgtagc ggccgatcag cgcgactgtt
     4681 gcgaagcgtt gtgccggacc ccccatggca gcgattaaag cacatgcgcg cgggcgggcc
     4741 aaacgcgcgc acagggtgtg gtcgtcgcct tgccgacaag gccggcggta caatagaccg
     4801 atcgtggatc ctcgctcgca acacctgtta aagacgctga tcgagcgcta catcaccgac
     4861 ggccagccgg tgggttcgcg tgcgctgtcg cggcaatcgg gcctggagct gtcgccggcg
     4921 acgatacgga acgtgatggc cgacctcgag gagatggggt tcatagccag cccgcacaca
     4981 tcggcgggca ggattcctac agccaagggt tatcgttttt ttgtcgatac gctgatggtc
     5041 gtgaagccgc tggagcaaat cgagatccgc cgtctcgaag aagagctcca cgccgatcac
     5101 ccgcagcaga tcgtcaatgc cgcggccgtc atgctgtcgc agcttaccca gttcgccggc
     5161 gtcgtaatga cgccgaagcg gcgcgaagcg acatttcgtc acctcgaatt cctgcgtctg
     5221 tccgagcgcc gggtcctgtt gatcatcgtg accgccgaaa acgacgtgca gaatcgcata
     5281 ctgcacacca atcgtccgta cacgcaggcg cagctgatcg aggcgacgaa tttcttcaac
     5341 cacaatttcg ccggctcgcc gctgggggcg atacgcggcc ggctggccga cgagctgcgc
     5401 gcgctgggcg acgacatcgc cgggctgatg agggcggcgg tcgatgtcgg cgaaggcgca
     5461 ttgagccgcg acgagtcgct ggtcgtcagc ggcgagcgga acctgttgaa cgccggcgac
     5521 ttctcgtcga acatgcagcg cctgcgcctg ctgttcgacc tgttcgagca gaagacgtcg
     5581 ctgttgcacc tgctcgacgc ctcgcagcaa gcggagggcg tgcagatcta tatcggcggc
     5641 gagtcgggtc tgctgccgct cgacgaatgc agtgtcgtca cggcgccgta cgaggcgggc
     5701 ggggaggtca tcggaacgct gggcgtcatc ggtccgactc ggatggccta tgagcgcgtc
     5761 gtgccgatcg tcgacgtcac ggccaagctg ctgtcgagcg cgctgtcgca gcagattgcc
     5821 gacgattgac cgcggccagc cgacgcgccg tctcgtgaac ggcatcgggc catcgcgctg
     5881 ccacgcgggc acacggtccc ttgcgcatcg cgagcgcgag ccggaggcat gctactgatg
     5941 tccttcctgc ccgagcccgc attcgcccat gaccggccgc gccggatcgg cgtgctgctg
     6001 gtcaacctcg gcacgcccga tgcgccgacg atccccgccg tgcgcgccta cctcgccgaa
     6061 ttcctgtccg atgagcgcgt aatcgagata ccgcgcgcgg cgtggctgcc catactgcac
     6121 ggggtgatcc tgcgcgtgcg gccggcgaag tcggctcgaa agtacgcgac catctggggc
     6181 aaggacggtt cgccattgct ggtccacagc gtcaagcaga agacgctgct gcagggctac
     6241 ctggggcagc ggctgaagga gatgggcctt ccgtccgatc acgcccagat cgagctcggc
     6301 atgcgctacg ggcggccgac gatagacagc gcgatggcca ggctcaaggc tggcggctgc
     6361 gaccgcgtgc tggtcctgcc gctttatccg cagtacgcgg cgagcaccac cgcgtccgcc
     6421 ctcgatgcgg tgttcgcgac ggcgtcccgg atgcgtgccg tcccggcgct gcgcgtggtc
     6481 gcgccttacc acgacgaccc cggctacatc aaagcggtcg cccagagcat caacgattac
     6541 tgggtgaaaa acggcaggcc gaacaaattg ctgctgtcgt tccacggcgt gccgaggaga
     6601 agcctcgacc tgggcgaccc gtaccattgt cactgccaca agaccgcgcg cctggtcgcc
     6661 agtgagctcg gtctgaccag cgagcagtac gcggtctcgt tccagtcgcg cttcggccgc
     6721 gcccggtggc tggagccgta tacgctgccg accctgctcg cgctggcgaa ggaggggatg
     6781 cgccgggtcg acatcgcgtg ccctggcttc gtcagcgact gcctggaaac cctcgaggag
     6841 ctgggccacg acgcgcaggc cgcctttcta ggcgccggcg gccaggaatt gcactgcatt
     6901 ccctgcctga acgagcgacc cgggtggatc gccgccctgg ccgacctggc gatgcgaaat
     6961 ctccagggct ggctggagtt gccggctgac gccgccgcgc gcgagatgag ccaggcgcga
     7021 gccaaagcct tgggcgcgga ccgctaggac gattcgcgtc cgggaccgtc tctcggggtc
     7081 caggcgggct cccgcgccgg gattacgctc ggattcgcgt cttttcggtc cgatcgcggt
     7141 tgaagcggat cgaaacagcc ccatatcccc ggtgttggca taggagcaac gatgggcgaa
     7201 aatcccgacc ataacagtca agtttccgga gaagtcgagg ccggcgttcc gatcccgacc
     7261 gatgcgccgg caggcgggcc ggcggcccgg ccgggcgatc ccgcgccgga tctggccacg
     7321 ctgctgaagc aggccgagga cgaagcttcc cggctcaagg atgcgtggct gcgcgcgatg
     7381 gcggaaaccg acaatgtgcg caagcttgcc cagaacgacg taaccaaggc gcacaagtac
     7441 gcgatcgagc gctttgccca ggagctcttg gttgtcaagg acgcgctcga gcagacgctg
     7501 gccgcgccgg ctgcatcgat cgaagcgctc agggatggcg tg
//
LOCUS       DATANS010000502         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_820579, whole genome shotgun sequence.
ACCESSION   DATANS010000502 DATANS010000000
VERSION     DATANS010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820579"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VHT22_07430"
     CDS             complement(<1..818)
                     /locus_tag="VHT22_07430"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX3137423.1"
                     /translation="MAGGPLKDIRVIDMTSVVVGPMVTLMLADYGADVIKIEPPSGDI
                     VRNLAGRAISPNMSPKFLHLNRNKRSLAIDLKKPDAYDAVKKLLATADVLLWNVRPSA
                     MARLKLSYEDVKAINPKIIYCGIVGFGQTGRYKNKPAYDSIIQGAGGIAALHHRATGE
                     PRYVPIVMADKTTGLIATQMVLMALYHRERTGEGQSIEVPMFENIARYVLEEHMYHQT
                     FDPPIGTTGDPRLFDPWARPVPTSDGYIGISANTDAQAFAVFDAIGKPEFKTDPR"
     gene            complement(1031..>1544)
                     /locus_tag="VHT22_07435"
     CDS             complement(1031..>1544)
                     /locus_tag="VHT22_07435"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX3137424.1"
                     /translation="LSYGSAGVGSGGHLAGELFRTTTGINATHVPYKGAAPASIDVAG
                     GQLAFQFASQITIQPLVAGKRVRMLAVTSAKRAASLAEVPSLAESGLAGFEVLNWFGV
                     AAPARTPPAVVTRLNAAIVKSLQLPGVREKLTSEGSEIVGNSPQEFTTFLKRDIARWA
                     QVIKDSGVKL"
BASE COUNT          252 a          477 c          533 g          282 t
ORIGIN      
        1 cgcggatcgg tcttgaattc cggcttgccg atggcgtcga acaccgcgaa cgcctgcgcg
       61 tcggtattgg ccgagatgcc gatgtagcca tcgctggtcg gcacgggccg cgcccatgga
      121 tcgaacaggc gcgggtcgcc ggtcgtgccg atcggcggat cgaaggtctg gtggtacata
      181 tgctcttcga gcacgtagcg cgcaatgttt tcgaacatcg gcacttctat cgactggcct
      241 tcgccggtgc gctcgcgatg atagagcgcc atcaatacca tttgcgtcgc gatcaaaccc
      301 gtggtcttgt cggccatcac gatcggcacg tagcggggct cgccggtcgc gcggtgatgc
      361 agcgccgcga ttccgccggc gccctggata atcgaatcgt aggcgggctt gttcttgtag
      421 cggccggtct ggccgaagcc gacgatgccg cagtaaatga ttttcgggtt gatcgctttg
      481 acgtcttcgt acgagagttt caaccgcgcc atcgcggacg gccgcacgtt ccacaggagc
      541 acgtcggcgg tcgcgagcag cttcttcacc gcgtcgtagg cgtcgggttt cttcaggtcg
      601 atcgcgagcg agcgcttgtt gcggttcaga tgcaggaact tcggcgacat gttgggcgaa
      661 atcgcgcgcc cagcgaggtt gcgcacgatg tcgccggacg gcggctcgat tttgatcacg
      721 tcggcgccgt aatcggcgag catcagcgtg accatcggcc cgacgacgac cgaggtcatg
      781 tcgatgacgc ggatgtcttt gagcggtccg ccggccatta gcgagactcc gcgcaggcca
      841 cgggtgcagc gtgagaatga tccgcagccg gcgcgcttgc gctgatctgg gcgggatagg
      901 cgaagttctg aatcactgcg gcgtctcctt gggggcggtg cgggacgggc ggaacgggcg
      961 gaacgggaaa aaagcggcga ggcgtgattc tatcaacttc acgcgtccgc cgtaccggcg
     1021 cgcggcgcgg ctaaagcttc acgccgctgt ctttgatgac ctgcgcccag cgcgcgatgt
     1081 cgcgcttgag aaacgtcgtg aattcctgcg gcgagttgcc gacgatctcc gagccttcgc
     1141 tcgtgagctt ctcgcgcacg ccgggcagtt gcagcgattt gacgatggcc gcgttaagcc
     1201 gggtcacgac ggcgggcggc gtgcgcgcgg gcgccgcgac accgaaccag ttcagcactt
     1261 cgaaccccgc cagccccgac tcggccagcg acggcacctc ggcgagcgat gccgcacgct
     1321 tagcgctcgt gacggcgagc atacgcactc gcttgcccgc gacgagcggc tggatggtga
     1381 tctgcgaggc gaactgaaac gcgagctggc cgccggcaac gtcgatcgat gccggcgcgg
     1441 cgcctttata gggcacgtgc gtcgcgttga tgccggtcgt cgtgcgaaag agctcgcccg
     1501 cgagatgacc gccgctgccg acgccggccg agccgtagct gagc
//
LOCUS       DATANS010000503         3364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_109022, whole genome shotgun sequence.
ACCESSION   DATANS010000503 DATANS010000000
VERSION     DATANS010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109022"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /gene="der"
                     /locus_tag="VHT22_07440"
     CDS             <1..1172
                     /gene="der"
                     /locus_tag="VHT22_07440"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX3137425.1"
                     /translation="PESLSGRMRAQTEAAIDQADIIFFMIDARAGPTPVDHDFADVAR
                     RSGKPIVLIANKSEGTAGEAGRLEAYALGLGEPIPISAEHDEGMSDLYDALHALVPDE
                     PAQEPVEDASDPEHPIRVAIVGRPNAGKSTLINRLIGEERLLTGPEAGITRDAIAVEH
                     DWQGHKFRIYDTAGLRRRSRVAEKLEKLSVADALNAVRFAEVVVVLLDVDSAFEEQDQ
                     RIADLIEQEGRAIVIAVNKWDLKEKTAGAIGKLREQAGEKLTQLKGVPLVAVSGLTGE
                     GLDRLMQAIIDAYEVWNKRIPTSALNRWFEHAVNANPPPAVAGRSLRLNYITQPKSRP
                     PSFVVFCTRADAVPDAYKRYLLNSMRENFDLPGTPIRLALREKDNPFKGRAKRKH"
     gene            complement(1178..1927)
                     /locus_tag="VHT22_07445"
     CDS             complement(1178..1927)
                     /locus_tag="VHT22_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3137426.1"
                     /translation="MADNSKPLSDRIALVTGASRGIGAAIALELAHAGAHVIAVSRTV
                     GGLEDLDDRIRAAGSSATLVPLDVKDSEGIARLALALNERYQRLDVLVGNAGILGPLS
                     PLSHVEPKDWDNLLAVNVTANWQLIRCMDVLLQRSQAGRAVFLTSGVAHMGRAYWGPY
                     AASKAALELLARTYASECATTPVRANLFSPGPTRTRMYASAFPGIDPLTLPTAEDVAK
                     EMLPLCLPECSETGRLYSFREKKFIDFHAPA"
     gene            complement(2031..>3364)
                     /gene="purF"
                     /locus_tag="VHT22_07450"
     CDS             complement(2031..>3364)
                     /gene="purF"
                     /locus_tag="VHT22_07450"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX3137427.1"
                     /translation="QEAAGIVSFDGARFHSERRMGLVGDTFSRREVIDRLPGRAAIGH
                     NRYSTTGETILRNVQPLFCELDGGGFAAAHNGNLTNGLTLRKQLVRDGAIMQSTSDSE
                     VILHLVARSQRNRFIDRFIDGLRQLEGAYSWVALTNKKLIGARDPLGIRPLVIGELDG
                     HPILASETCALDIINARYVRDVENGEVVVFDESGVQSHKPFPLLQPRPCIFEYVYFSR
                     PDSIVGGRAVYDVRKTMGAELAREAPAQADVIVPVPDSGVPAAIGFAQEAKIPYELGI
                     IRNHYVGRTFIQPTQAIRDQGVRLKHSANRAVVEGRRIVLVDDSIVRGTTSRKIVQMM
                     RDAGAKEVHFRIASPPITHPDYYGIDTPERDKLLAATHDLEGMRKFIGADSLAFLSVD
                     GIYRAMGFQGRDPARPQFTDHCFTGDYPTALTDQAAQEASPRQLSLLAEAI"
BASE COUNT          617 a         1061 c         1116 g          570 t
ORIGIN      
        1 caccggaaag tctttccgga cgcatgcggg cgcagacgga agcggcgatc gatcaggccg
       61 acatcatatt tttcatgatc gatgcacgcg cgggcccgac gccggtcgat cacgacttcg
      121 ccgatgtcgc gcgcagatcc ggcaaaccga tcgtgctgat tgcgaacaaa agcgaaggaa
      181 cggccggtga agcaggccgc cttgaagcct atgccctcgg cctcggggag ccgatcccga
      241 tctctgccga gcacgacgag ggcatgtccg acctttacga cgccctgcac gctttggtgc
      301 cggacgaacc cgcgcaggag ccggtcgaag acgcatccga tccagagcac ccgatccgcg
      361 tcgccatcgt cggccgtccc aacgccggga aatcgacgtt gatcaaccgg ctgatcggcg
      421 aggagcggct gctgaccgga ccggaagccg gcatcacccg cgatgccatc gcggtcgagc
      481 acgactggca gggtcacaag ttccggattt acgacaccgc cggattgcgt cgccgctcgc
      541 gggtcgcgga aaagctggag aagctttccg tcgcggacgc gctcaatgcg gttcgcttcg
      601 ccgaagtcgt cgtcgtgctt cttgacgtag acagcgcctt tgaagagcag gaccagcgca
      661 ttgccgatct gatcgaacag gaagggcgcg ccatcgtcat cgcggtcaac aaatgggact
      721 tgaaggaaaa gaccgcggga gccatcggca agctgcgcga gcaggccggc gaaaagctga
      781 cccagctcaa gggtgtgccc ttggtggcgg tctcaggcct caccggcgaa ggcctcgacc
      841 ggctgatgca ggcgatcatc gatgcctacg aggtctggaa caagcgcatc ccgaccagcg
      901 cgctcaatcg ctggtttgaa cacgccgtca acgcgaatcc accgccggcg gtggccggcc
      961 gctcgctgcg tctcaactac atcacgcagc cgaaaagccg tccgccgagt ttcgtcgtgt
     1021 tctgcacgcg cgcggatgcc gtgcccgacg cgtacaagcg atatttgctg aattcaatgc
     1081 gtgagaattt cgatttgccg ggtacgccca ttcgtctcgc cttgcgcgag aaggacaacc
     1141 cgttcaaggg acgggcaaaa agaaagcact gagcgcgtca ggccggcgcg tggaaatcga
     1201 taaatttctt ttcgcggaaa ctgtagagcc tgcctgtttc actgcattcg ggcaggcaca
     1261 gggggagcat ttccttcgcg acgtcctccg ccgtcggcaa ggtcagcgga tcgatgcccg
     1321 ggaaggcgct cgcatacatg cgcgtgcgcg tcggcccggg gctgaacagg ttcgcgcgca
     1381 ccggtgtcgt cgcgcactcc gatgcgtagg tgcgcgcgag gagttccagc gccgccttcg
     1441 acgccgcgta cggcccccaa taggcccgtc ccatatgcgc gacgccggac gtcagaaata
     1501 cagcgcgacc ggcttgcgac cgttgcagaa gcacatccat gcagcggatc aattgccagt
     1561 tcgcggttac gttgaccgcg aggagattgt cccaatcttt cggttcaaca tggctcagcg
     1621 gggacagtgg ccccagaatc ccggcattgc cgaccagtac atcgaggcgc tgatatcgct
     1681 cattgagcgc cagcgcgagg cgcgcaatgc cttcgctatc cttgacgtcg agcggtacca
     1741 gcgtggcgga gctgccggcc gccctgatcc tgtcatcgag gtcttcaagc ccgccaaccg
     1801 tgcggctcac cgcaatcacg tgtgcgccgg catgcgccag ctcaagcgcg atagcggcgc
     1861 cgatgccgcg cgaggcgccg gtgacaagcg cgatacggtc ggaaaggggc ttggaattat
     1921 ctgccattca gcagtcaaac ccggccggcg ccgggtaccc acgtcttttc aatgcaggct
     1981 ttagaagacg tgcgccgcgg gaacaagccc ggtcacggcc tgggttgggt tcagatggct
     2041 tcggccagga gcgatagctg tcgcgggctg gcttcctggg cggcctgatc ggtgagggcc
     2101 gtcgggtagt cgccagtgaa gcagtgatcg gtgaactgcg gccgcgccgg gtcgcgtccc
     2161 tggaaaccca tcgcgcggta gatgccatcg accgacagga aggcgaggga atccgcgccg
     2221 ataaatttgc gcatgccctc gaggtcgtgc gtggcggcga gcaatttgtc gcgctcgggc
     2281 gtatcgatcc cgtagtagtc cgggtgcgtg atcgggggcg acgcaatgcg gaagtgcact
     2341 tctttcgcgc ccgcatcgcg catcatctgc acgatcttgc gtgaggtggt gccgcgcacg
     2401 atcgaatcgt cgacgagcac gatccgccgg ccttccacca ccgcccggtt ggccgagtgc
     2461 ttgaggcgaa ccccctgatc gcgaatagcc tgtgtgggct gaatgaaggt tcgaccgaca
     2521 tagtgattgc ggatgatgcc gagctcgtag ggaatctttg cttcctgagc gaaaccgatc
     2581 gccgccggca cgccggagtc aggcacgggc acgatgacgt cagcctgcgc gggtgcctcg
     2641 cgtgcgagtt cggcgcccat ggtcttgcgc acgtcgtaga cggcgcggcc gccgacgatc
     2701 gagtccggcc gggagaaata gacgtattcg aagatgcacg ggcgcggctg cagcagcgga
     2761 aacggcttgt gcgactgcac gccgctctcg tcgaacacca cgacttcgcc gttctcgacg
     2821 tcgcgcacat agcgcgcatt gatgatgtcg agcgcgcagg tctccgaggc gaggatcgga
     2881 tggccgtcga gctcgccgat caccagcggg cggatgccga gcgggtcgcg cgcgccgatc
     2941 agcttcttgt tggtcagcgc cacccaggag taggcgccct cgagctggcg cagcccgtcg
     3001 atgaagcgat cgatgaagcg gttgcgctgt gagcgcgcca ccagatgcag gatcacctcc
     3061 gagtcgctgg tcgactgcat gatcgcgccg tcgcgcacca gttgcttgcg cagcgtgagg
     3121 ccgttggtga ggttgccgtt gtgcgcggcc gcgaagccgc cgccgtccag ctcgcagaac
     3181 agcggctgga cgttgcgcag gatcgtctcg ccggtggtcg agtagcgatt gtggccgatc
     3241 gcggcgcgcc ccggcaggcg gtcgatcacc tcgcggcgcg agaacgtatc gccgaccagg
     3301 cccatgcgcc gctcggaatg gaaccgggcg ccgtcgaacg acacgatgcc ggccgcctcc
     3361 tggc
//
LOCUS       DATANS010000504         4576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_151680, whole genome shotgun sequence.
ACCESSION   DATANS010000504 DATANS010000000
VERSION     DATANS010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151680"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VHT22_07455"
     CDS             <1..494
                     /locus_tag="VHT22_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HEX3137428.1"
                     /translation="DFDAPLAAAVRAGRLKEFARFAHFADAASVAAIPDPQAPTTFAQ
                     SKLDWDSSTDSEHARWLDRYRELLKVRRDAVIPMLAKLDPQRCGFAITGPRSLAAHWS
                     TDDGARLLLFANLGGAPDAGPALPPGKLVYTTGPTAAQALVSTWSTTWLLIDERTAET
                     RDA"
     gene            487..2697
                     /gene="malQ"
                     /locus_tag="VHT22_07460"
     CDS             487..2697
                     /gene="malQ"
                     /locus_tag="VHT22_07460"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX3137429.1"
                     /translation="MPERAILEHLCERYGIAAGYEDAWGVSRQTPEDTKLALLRAMGI
                     DVAAAANDASGADPDARASGEPAPAIVVDEREGRITLRLGPSSSVDWRIDLETGECLE
                     GVAAPASVADGDGRSAFEIPLPQATGYHTLTIRDHATGARLASSPLIVTPSRCYSPPL
                     LAGGSRIWGLALQLYALRSHRNWGMGDFTDLRNAVGVAAATGADFVGTNPLHALFPAR
                     PEAASPYSPSNRAALNVLYIDVEAVHDFVDCKAARARVYSPTFQSTLARLRAFDHVDY
                     VAVARLKFEILALLYDHFREHQLGSSPGERGHAFREFQRERGPALKLHGLFDALHEHI
                     AGITGDRGGWRSWPAEYRPPSATGDDELYRRHAERAEYFQYLQWQADLQLGAASEHAR
                     TCGLAIGLYGDFAVGADSGGAETWIQSAVYAPDMNIGAPPDDFNLGGQDWGLPPMLPQ
                     RLKAAGYAPFITALRAAMRHAGALRIDHVMALMRLFWVPQGTVPVDGAYVAYPFSELL
                     GIVALESSRNHCLVIGEDLGTIAPGVRAALGTAGVLSYRPLYFERTGDAEFAPPESYP
                     AQALVTIGTHDLPTLSGFWAGRDIAARRRLRLYPDENAPVEQLAARSRDRGRLMRALE
                     RAGVLAAADAPRRMTVDLMIAVHRFLARTPAAVMAVQLEDVFGQDTQVNLPSTTEDQY
                     PNWRRKLNIPLESWASNRRFAALTRMLRKERRRRAPHDPHRPAGDGWSLGRDAG"
     gene            2694..>4576
                     /gene="treY"
                     /locus_tag="VHT22_07465"
     CDS             2694..>4576
                     /gene="treY"
                     /locus_tag="VHT22_07465"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX3137430.1"
                     /translation="MNIPRATYRLQLHRDFGFADATALVPYLAELGISHIYLSPWLKA
                     RPGSTHGYDIVDHGRLNPELGSDDDFAALAASLAAHAMGQMADIVPNHMGVMGSDNAW
                     WLDVLENGRASRFAGYFDIDWTPPLAALSDKVLVPVLADNYGEVLLRGELQLGFDSET
                     GEFSIRYFQHRFPLDPRSYAPILHDAAAPGAIPRGQAAYRMVDLAHAFAALPGHDHAT
                     PRRLARRWRDKERLKQQLAALAAGTPEIRKRIGDAVARLNGRPGDAASFDALHALLEV
                     QAFRLTGWRVAADDINYRRFFDINELAALRAEDPQVFTESHRLVLRLVGERKIDALRI
                     DHPDGLYDPKAYFERLQTAVRSALLPSGAKRAQRESPSPAAALYTTVEKILAEHEALP
                     ADWPVQGTTGYRFANLVGGLWVDGAAKSRFDRVYAAFIGGWVDFDQILREAKLLIMSG
                     ALASDLNLLAHALTRIAEADRNTRDLSFNVLRRTVMLYTAALPVYRTYIDGRGPGASD
                     LRYIDWAIGVARQWRPAPDRIAFDFLRRVLTGEDAAAPGPLRADIDRFARRLQQFTGP
                     VMAKAMEDTSFYVYNRLVALNEVGGDPDVFGVSPAEFHATAGRLARRWPATMTTLSTH
                     DTKR"
BASE COUNT          727 a         1618 c         1478 g          753 t
ORIGIN      
        1 gcgatttcga cgcgccgctt gctgcggcag tgcgcgccgg cagactcaag gagttcgcgc
       61 gcttcgcgca ctttgccgac gccgcctccg tggcggcgat tcccgatccg caggcgccga
      121 cgacgttcgc tcagagcaaa ctcgactggg actccagcac ggattccgag catgcacgct
      181 ggctcgaccg gtatcgcgag ctgctgaagg tcaggcgcga cgccgtgatc ccgatgctgg
      241 ccaaactcga tccacagcgg tgcggtttcg cgatcaccgg tccgcgcagc cttgccgcgc
      301 actggtcgac cgacgatggc gcccgcctgc tgcttttcgc aaacctgggc ggcgcacccg
      361 acgccggacc ggcgctgcct cccggcaagc tcgtctacac gacaggccct acggccgcgc
      421 aggcgctcgt ctcgacctgg tcgacaacct ggttgctgat cgacgagcgc accgcggaga
      481 cacgcgatgc ctgagcgcgc gatcctcgag cacttgtgcg agcgttacgg catcgcggcc
      541 ggctacgagg atgcgtgggg cgtctcgcgc cagacgccgg aagacaccaa gcttgcgctg
      601 ctgcgggcga tgggaatcga cgtcgccgcg gcggccaacg atgcctcggg tgccgacccc
      661 gatgcccgcg caagcggcga accggcgccg gccatcgtcg tcgacgaacg cgagggcaga
      721 atcacgctgc ggctggggcc atcgagctca gtcgactggc ggatcgatct ggaaaccggc
      781 gaatgcctgg aaggcgttgc cgcgccagca tcagtggccg atggcgacgg tcggagcgcc
      841 ttcgagatcc cgctgccgca ggccaccgga tatcacacgc tgacgattcg cgaccacgcg
      901 acaggcgcgc ggcttgcatc gtcgcctttg atcgtcacgc cttcgcgttg ctacagcccg
      961 ccgctgctcg ccggcggctc ccggatctgg ggattggcgc tgcagcttta cgcgctgcgt
     1021 tcgcatcgca actggggcat gggtgacttt accgacttac gcaacgccgt cggcgtcgcg
     1081 gccgccacag gcgccgactt cgtcggcacc aatccgctgc acgcactgtt tcccgcccga
     1141 ccggaagcgg cgagccccta cagcccatcg aatcgcgccg ccttgaacgt gctctatatc
     1201 gacgtcgagg cggtccacga cttcgtcgac tgcaaggccg ctcgggcgcg cgtctacagt
     1261 cccaccttcc agagcacgct cgcacgcttg cgagcgttcg accatgtcga ctacgtcgcg
     1321 gtcgcgcgac tgaagttcga gattctcgcg ctgctatacg atcacttccg cgagcatcag
     1381 ctcggctcga gcccgggcga gcgcggtcac gccttccgcg aatttcagcg cgaacgcggg
     1441 ccggcgctca agctgcatgg gctgttcgat gcactgcacg agcacatcgc ggggatcaca
     1501 ggcgatcgcg gcggctggcg gagctggccg gccgagtatc gcccgccgtc ggccaccggc
     1561 gatgacgagc tctatcgccg ccatgccgag cgcgccgagt atttccagta cctgcagtgg
     1621 caggcggact tgcagctcgg cgcagcgagc gagcacgccc gcacctgcgg ccttgcgatc
     1681 ggactgtacg gcgattttgc cgttggcgcg gactcaggcg gcgcggaaac gtggattcag
     1741 tccgccgtct acgcgccgga catgaacatc ggcgccccgc cggatgactt caatctgggc
     1801 ggacaggact ggggcttgcc gccgatgctg ccgcagcgtc tgaaggccgc aggctacgcc
     1861 ccgtttatca ccgccttgcg cgcggccatg cgccatgcgg gcgcattgcg catcgatcac
     1921 gtcatggcgc tcatgcgctt gttctgggtg ccgcaaggga ccgtgcccgt cgacggcgcc
     1981 tacgtcgcct atcctttttc cgaactgctg ggcatcgtcg cgctcgaaag cagtcgcaat
     2041 cactgcctcg tcatcggcga ggacctgggg acgatcgcgc ccggcgttcg cgccgcgctc
     2101 ggaacggcgg gagtgctctc ctaccggccg ctgtatttcg agcgcaccgg cgacgccgaa
     2161 ttcgcgccgc ccgaaagtta tccggcgcag gcgctcgtca ccatcggcac gcacgacctg
     2221 ccgactctat ccggtttctg ggccgggcgc gacatcgccg cgcgcaggag gctgcgcctg
     2281 tatcccgacg agaatgcccc ggtggaacag ctggctgcgc gatcgcgcga tcggggacgg
     2341 ctgatgcgcg cgctcgagcg cgccggggtt ctcgccgccg ccgatgcgcc gcggcgaatg
     2401 accgtcgatc tgatgatcgc cgtgcatcgt ttcctggccc gcacgccggc ggcggtcatg
     2461 gccgtgcaac tcgaggacgt tttcggccag gacacgcagg tcaatctccc cagcaccacc
     2521 gaagaccaat atccaaactg gcggcgcaag ctgaacatcc cgctcgaatc ctgggcgtcg
     2581 aaccgacgct tcgccgcgct cacccggatg ctgcgcaagg aacggcgacg gcgggcaccg
     2641 cacgacccgc atcgccctgc tggcgacggc tggtcgctcg gccgcgatgc gggatgaata
     2701 tccctcgagc gacctatcgc ctgcagctgc atcgcgactt cggattcgcc gacgccaccg
     2761 cactggtccc gtatctcgcc gaattgggca tcagccacat ctatctgtcg ccctggctca
     2821 aggcgcgccc cggaagcacg cacggctacg acatcgtcga tcatggccga ctcaatcccg
     2881 aactgggcag cgacgatgac tttgccgcgc tcgccgcttc gcttgccgcc cacgccatgg
     2941 ggcagatggc cgacattgtg cccaatcaca tgggcgtcat gggcagcgac aacgcctggt
     3001 ggctcgatgt gctcgagaac gggcgcgctt cgcgattcgc cggctacttc gacatcgact
     3061 ggacgccgcc gctggccgcg ctttcggaca aggttctggt tccggtgctg gccgacaatt
     3121 acggcgaggt gctgctccga ggcgagctgc agcttggatt cgattcggag accggcgaat
     3181 tcagcattcg ctatttccag catcgctttc cgctcgatcc acgctcgtac gcgcctatcc
     3241 tgcacgatgc ggcggcgccg ggtgccatcc cccgcggcca ggcggcgtat cggatggtcg
     3301 atctggcgca tgcattcgcg gcgttgcccg ggcatgacca tgccacgcca cgccgactcg
     3361 cccgacgatg gcgtgacaag gagcgcctga agcagcagct cgcggcgctg gcggccggca
     3421 ccccggaaat ccgcaagcgt atcggcgacg ccgtggcgcg gctcaatggc cgcccgggcg
     3481 acgcagccag cttcgacgcg ctgcacgcgc tgctcgaagt ccaggcgttc cgcctgaccg
     3541 gctggcgagt cgcagcggat gacatcaact accgccgatt cttcgacatc aacgagctcg
     3601 ccgcgttgcg cgccgaagat ccgcaggtct tcaccgaatc gcatcggctg gtgctgcgac
     3661 tggtgggcga gcgcaagatc gacgcgctgc gcatcgacca tcccgatggc ctgtacgatc
     3721 cgaaggcgta tttcgaacgg ctgcagaccg ccgtgcgctc cgcgctgctg ccaagcgggg
     3781 caaagcgcgc gcagcgcgaa agcccctcgc ctgcggccgc gctgtacacc accgtggaaa
     3841 aaatcctggc cgagcacgaa gccttgccgg cggattggcc ggtacagggg acgaccggct
     3901 accggttcgc caacctggtc ggtgggctgt gggtcgacgg cgcggcaaag tctcgattcg
     3961 atcgtgtcta cgccgccttc atcggcggat gggtcgattt cgatcagatc ctgcgtgaag
     4021 cgaaattgct gatcatgtcc ggcgccctcg catccgacct caacctgctc gcgcacgcgc
     4081 tgacccgcat cgccgaggcc gaccgcaaca cacgcgatct ctcgttcaat gtcctgcgtc
     4141 gcacggtaat gctctacacc gcggcgctgc cggtctaccg cacctacatc gacggccgcg
     4201 gccctggcgc gagcgatttg cgctacatcg actgggcgat cggtgtcgcc cggcaatggc
     4261 ggcctgctcc cgatcggatc gccttcgatt tcctccgccg ggtgctgacc ggcgaggatg
     4321 ccgcggcgcc gggcccgctg cgcgctgaca tcgatcgctt cgcgcgccgg ctgcagcaat
     4381 tcaccgggcc ggtcatggcg aaggcgatgg aagacacgag cttctatgtg tacaaccggc
     4441 tggtcgcgct gaatgaggtc ggcggcgacc ccgacgtctt cggcgtcagc ccagccgagt
     4501 tccacgccac cgccgggcgc ctggcccggc gctggcccgc caccatgacc acgctgtcca
     4561 cccacgacac caagcg
//
LOCUS       DATANS010000505         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_781385, whole genome shotgun sequence.
ACCESSION   DATANS010000505 DATANS010000000
VERSION     DATANS010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781385"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..>2126)
                     /locus_tag="VHT22_07470"
     CDS             complement(201..>2126)
                     /locus_tag="VHT22_07470"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX3137431.1"
                     /translation="CAPRVKPCMENPAPDPGSTRSGDAAAQTCARIRKHLEQGAPWDA
                     CDLFREEIAKHPGDAMLLYWGALAHARSGGSKRAHALLDQAETADNGKEHRADILSLR
                     GRLWKDDYQREPDQPEGRTKAERARQEYLAAYALKHDVYPGINGATLSLLLGDRPAAL
                     TLAQQIVERLAGQASRRTFWDDATLGEAQLVLGQFDQAAQSYASAYAQSAGDTGSVAT
                     MRRQLHLLARAVPQAADALRQLPAPDIVAFAGHMIDAPDRALPRFPAALVPAVQAAVR
                     DHVARLNTPIVYTSAACGADLIFIEAALEAGAEVNVVLPFDRDDFVQTSVAVGGEGWV
                     DRFDAALAQATRVIMATDERYLGDDVLFEHAALLLEGLSILRGAQLQTIPSLVCVIDA
                     AAPGRVGGTQASFDRWTRHVGPPEVLDLAKMRSNAALAGPAVTPVAPHPDEATPGPPQ
                     SPAGYSRPIAAKRPQRSLKTLLFADFAGFSRVHDAVAPFFQESFWKIAAGQIAASPAK
                     PLLASTWGDALYVVFDAPRNGAAFALSFLDNMLEVDWASLGLSEASPIRIALHAGPVF
                     CGFDPIIGRDNYFGSSVTKAARIEPVTPPGMVYASEAFAATLAATERDEFSLEYIGRM
                     ALAKSYGESRIYRLDRR"
BASE COUNT          369 a          726 c          686 g          345 t
ORIGIN      
        1 cgaacgtcca ccgatcgccg accgaccaga caggtaacgc accctcctct gcagcggccg
       61 gcactaaagc cattgtcaaa agcagcgcca tccaggcgcc tcgtcgttgc ctcttcactg
      121 cactctccag aacgagacgc gatggtagta tgaaaatcat tgcgacaccg gccaatcctt
      181 gcatgcgggc tcttccgtct tcagcgccgg tccagccggt aaatgcgcga ctcgccataa
      241 ctcttggcca gcgccattcg tccgatgtat tcgagcgaaa attcatcgcg ttccgtcgca
      301 gccagcgtgg cggcgaatgc ctcgctggcg tacaccatgc ccggaggcgt caccggctcg
      361 atgcgcgcag ctttggtgac actcgagccg aaatagttgt cgcggccgat gatcggatcg
      421 aaaccgcaga ataccggtcc cgcgtgcagc gcaatccgga tcgggctggc ctccgacaag
      481 ccgagagacg cccagtcgac ctcgagcatg ttgtcgagaa aggacagcgc gaacgccgcg
      541 ccgttgcgcg gggcgtcgaa cacgacgtac agcgcatcgc cccaggtgct ggccagaagt
      601 ggcttggccg gcgaggccgc gatctgcccg gccgcaatct tccaaaagct ttcctggaag
      661 aacggcgcca ccgcgtcgtg cacgcggctg aacccggcga agtcggcaaa cagcaaggtc
      721 ttgagcgaac gctgcggtcg cttggccgcg atcggcctcg aatatccggc aggtgattgc
      781 ggtgggccag gcgtcgcctc gtcggggtgc ggcgcgacgg gcgtgacagc cggtccggcg
      841 agcgcggcgt tggaccgcat cttggccaga tccaacacct ccggcggccc gacatggcgc
      901 gtccaccggt cgaaagaggc ctgcgtgcca ccgacccgcc ccggcgccgc ggcgtcgatg
      961 acgcaaacca acgagggaat cgtttgcagc tgcgcgccac gcagtatcga aagtccctcg
     1021 agcagcagcg cggcatgctc gaacagaacg tcgtctccca gatagcgctc atcggtcgcc
     1081 atgatcacgc gcgtcgcctg ggccagcgcg gcatcgaagc gatccaccca tccttcgccg
     1141 ccgacggcca cgctggtctg cacgaagtcg tcgcggtcga acggcaacac gacgttgact
     1201 tcggcgccgg cctcgagcgc agcttcgatg aaaattagat cggcgccgca ggccgccgag
     1261 gtgtagacga tcggcgtatt cagccgggcg acatggtcgc ggaccgccgc ctggacggcg
     1321 gggactagcg ccgccgggaa ccgcggcagc gcccgatccg gcgcgtcgat catatggccg
     1381 gcaaacgcaa cgatgtcggg cgcgggcagc tgccgaagcg cgtcggccgc ttgcggaacc
     1441 gcgcgagcga gaaggtgcag ttgacgccgc atcgtagcca cgctccccgt atcgccggcg
     1501 ctttgggcat aggccgacgc atagctttgc gcggcctggt cgaattgacc gaggacgagc
     1561 tgcgcctcgc ccagcgtcgc atcgtcccag aatgtgcgcc ggcttgcctg ccctgccagc
     1621 cgctcgacga tctgttgtgc cagcgtcaac gccgccggac ggtcgccgag cagcaacgag
     1681 agcgtcgccc cgttgatgcc gggatagacg tcatgtttca gcgcataggc ggccagatac
     1741 tcctgacgcg ctcgctcggc cttcgtcctt ccttcgggct gatcgggctc gcgctgataa
     1801 tcgtctttcc agaggcgccc gcgcaagctc aggatgtcgg cccgatgctc ttttccgttg
     1861 tcggctgtct ccgcctgatc cagcaaggca tgcgcgcgct tcgaaccgcc tgatcgcgcg
     1921 tgcgccagtg cgccccagta gagcagcatc gcgtcgcccg ggtgcttcgc tatctcttcg
     1981 cggaaaagat cgcaggcgtc ccacggggct ccctgctcga ggtgcttgcg gatgcgggcg
     2041 cacgtttgag cggccgcatc gccggaccgc gtcgagcctg ggtcgggagc cgggttctcc
     2101 atgcagggct tcacacgcgg cgcaca
//
LOCUS       DATANS010000506         2715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_279091, whole genome shotgun sequence.
ACCESSION   DATANS010000506 DATANS010000000
VERSION     DATANS010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279091"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="VHT22_07475"
     CDS             complement(<1..1053)
                     /locus_tag="VHT22_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEX3137432.1"
                     /translation="MSVLLLLPAVAGAAPVEPPAIAGVPIDFLLFGLTLIGVALFHRH
                     TLPIAVGGMVVISLFKIAFSPFAEGPGVAGWLDHLGHEWVLLTNLLGLLLGFALLSKH
                     FEASRIPQWLPKLLPDDWKGGLVLLFMIFVLSSFLDNIAAALIGGTIAGAVFRGKIHI
                     GYLAAIVAASNAGGSGSVVGDTTTTMMWIDGVHPLQVLEAYIAAGAAMLVFGIPAAIQ
                     QQRYSPITREAPAGVVIDWARVFIVALILVTAIVVNVVVNVHYNDIAGSFPFIGAGVW
                     VALLICVPLRRPAWSELPGALRGSIFLLSLVLAASMMPVHKLPDASWHVALGLGFVSA
                     VFDNIPLTALALRQGGY"
     gene            1403..>2715
                     /locus_tag="VHT22_07480"
     CDS             1403..>2715
                     /locus_tag="VHT22_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX3137433.1"
                     /translation="MALPRSFTRLDLPTQQTVGEGLDRESLLALSQRKLNALGLPVCY
                     IDGDQRYRFVNRAFLDWSGKPQNEVIGREVAEVDGRELYQLYHAYLEAALNGERVTFE
                     RQLSSVKRNAFWVRVDYYPDRGPRGDIRGVLATYTDVDNIKRMELEAGEREHRLRIVT
                     DSVSLPIFYFDRSFRLRFANKPYGEYIGVAVDDLLGQPLKNFIAADALDEMMGYVERA
                     FAGATVSYDRRERPASGDLRWVRITLFPDREPGRKTGGAFAVINDIEDDVRIREALKS
                     QESQLRLFADNIPGPIAYLDRSLKYTFVNQAFANWVCRPQAEIYGKTPYQVMPHDVNA
                     FLRPIIKRALEGENVEYERVGASADGKRRWMHGRIAPDLDVAGSVRGLYCTEYDIHDL
                     KLTEQALATREQQLRLFTDNIPEPVVYLDAERRYVFVNEAFLRLYG"
BASE COUNT          533 a          908 c          855 g          419 t
ORIGIN      
        1 gtagccgccc tggcgcaacg cgagcgcggt cagcggaatg ttgtcgaaca ctgccgacac
       61 gaagcccagt cccagcgcga catgccacga agcgtcgggg agcttgtgca ccggcatcat
      121 cgacgccgcg agtaccaaag acagcaggaa aatgctgccg cgaagcgcac cgggaagctc
      181 gctccaggcc ggacgccgca gaggcacgca gatcagcagc gcgacccaca cgccggcgcc
      241 gataaaggga aagctgccgg caatgtcgtt gtaatgcacg ttgacgacga cgttgacgac
      301 gatcgcggtc acgagaatca gcgcgacgat gaacacccgg gcccaatcga tgacgacacc
      361 ggccggcgct tcgcgcgtga tcggcgagta gcgctgttgc tggatcgccg ccggtatgcc
      421 gaagacgagc atcgcggcgc cggcggcaat gtaggcttcg agcacctgca gtggatggac
      481 gccgtcgatc cacatcatcg tcgtcgtcgt gtcgccgacg acgctgcccg acccgccggc
      541 gttggacgct gcgacgatcg ccgccaggta gccgatgtgg atcttgccgc gaaacaccgc
      601 gccggcgatc gtgccgccga tcagcgctgc ggcgatgtta tccaggaagc tcgacagcac
      661 gaaaatcatg aaaaggagga ccaagccgcc tttccagtcg tcgggcagca gtttgggcaa
      721 ccattgcgga atgcggctgg cctcgaagtg cttggacagc agggcaaagc cgagcagcag
      781 gcccaacagg ttggtgagca gcacccactc gtgcccgagg tgatcgagcc agccggcaac
      841 gccgggtcct tccgcgaacg gcgaaaacgc aatcttgaac agcgatatca cgaccatccc
      901 gccgacggcg atcggcagcg tatggcggtg aaacaacgcg acgccgatca aggtcagccc
      961 gaacaggaga aaatcgatcg gcacaccggc aatcgccggc ggttcgaccg gcgccgcccc
     1021 ggccacggca ggcagcagca acagcacgga catcgccagc cagcgggctg ccaagctcaa
     1081 gacgcggggc atcgacttcc tcctgaattc cggcggccgt cgcgacgttg cgtggcgcgg
     1141 aagggcctgg aagcacttct ggctgaccgc cgaaagcagc agtagctgac aaaaagtgcc
     1201 gcccggcggg tttttgtgcc gctgattgta gcccgtcgcc gtcgacccgc caattcgagg
     1261 gccaaaaacg ctgattttgg cccattttca ctcgcgctcc ccggaatggc agactacttg
     1321 cttgtaagtg gcggattcga cacttagact gattgaccgc cggtctcccg ggagtggagg
     1381 caggcggtcc gaccccggtg ccatggcgct accccgctca ttcactcgcc tggacctgcc
     1441 gacgcagcaa acagtcggcg aaggtctgga cagggagtcc ctgctagcgc tgtcccagcg
     1501 caagctgaac gcgctcggcc tgccggtctg ctacatcgac ggcgaccagc gctaccgctt
     1561 cgtcaatcgc gctttcctcg actggtctgg caagccgcaa aacgaggtga tcggccgcga
     1621 ggtggccgag gtcgacggcc gcgagctcta ccagctctat cacgcctatc tcgaagccgc
     1681 gctcaacggc gagcgagtga ccttcgagcg ccagctttcg tcggtcaagc gcaatgcctt
     1741 ctgggttcgc gtcgactact accccgatcg cgggccgcgc ggcgacattc gcggcgtgct
     1801 cgcgacttac accgacgtcg acaacatcaa gaggatggag ctcgaggcgg gcgagcgcga
     1861 gcatcgcctg cgcatcgtca ccgacagcgt cagcctgccg atcttctatt tcgatcgctc
     1921 gttccgcctg cgctttgcca acaagcccta cggcgaatac atcggcgtcg cggtcgacga
     1981 tcttctgggc cagccgctga agaatttcat tgcggccgac gcgctcgacg agatgatggg
     2041 atatgtcgag cgcgccttcg ccggcgcgac cgtcagctac gatcgccgcg aacgcccggc
     2101 cagcggcgac ctgcgctggg tacgcatcac gctgttcccc gatcgcgagc ccggccgcaa
     2161 aaccggcggc gcgttcgcgg tgatcaacga catcgaggac gacgtccgca ttcgcgaggc
     2221 gctgaagtcc caggaatcgc agctgcggct gttcgccgac aacattcccg gcccgatcgc
     2281 ctatctcgat cgctcgctca agtacacgtt cgtcaaccag gcgtttgcca attgggtctg
     2341 ccggccgcag gcggagatct acggcaagac gccgtaccaa gtcatgccgc acgacgtcaa
     2401 cgcgtttttg cggccgatca tcaagcgcgc gctcgagggc gagaacgtcg agtacgagcg
     2461 ggtaggagcc agcgccgacg gcaagcgccg ctggatgcac ggccgcatcg cgcccgacct
     2521 cgacgtcgcg ggatcggtgc gcgggctcta ctgcaccgag tacgacatcc acgacctgaa
     2581 gctcaccgag caggcgctcg cgacgcgcga gcagcagctg cgtctgttca ccgacaacat
     2641 ccccgagccg gtcgtctacc tcgacgccga gcgccgctac gtgttcgtca acgaggcatt
     2701 cctgcgcctc tacgg
//
LOCUS       DATANS010000507         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_24721, whole genome shotgun sequence.
ACCESSION   DATANS010000507 DATANS010000000
VERSION     DATANS010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24721"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..1456
                     /locus_tag="VHT22_07485"
     CDS             197..1456
                     /locus_tag="VHT22_07485"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX3137434.1"
                     /translation="MSSDAGVLLLREVERRLGIAARLAACVPDRRDPERISHGLEEML
                     RLRMFAIAAGYEDADDCDSLRHDPAFKMAVGRLPESGDPLCSQPTMSRLENTPSRIAI
                     ARMMLAMIEVFCTSYARAPAVIVLDIDDTLDRVHGQQQLALFNAHYNERCFLPIHIYE
                     GTSGKLVTTILRAGKTPGGEEVRTVLKHVIKRIRQHWPRVSVLVRGDSHYGRDAAMTW
                     CEANKVAYIFGLAGNATLAYMTQPVAEELRVRRAAYDAEKLRRCCALRYGAKSWPVQR
                     HVVARVEATRLGLDVRYIVTSLAGTPEHLYETVYCARGQAENYIKLHKAQLASDRTSC
                     RDPRANQVRLILHGAAYWLLYMLRAAAPKRSPLRRAEFTTLRLRLIKIAARVVEGAAR
                     IRVWLPTVCLDRATFALLAGRFAAAGP"
BASE COUNT          280 a          561 c          580 g          263 t
ORIGIN      
        1 tctttattca cgccaatagg ggagcggagc gacgggcgtc gcggaaagcc gcaattcgat
       61 tcatgattct ggttgcgaga cagcgtcatg gtcggattga ggagcgccac acccgtcatg
      121 gatcaggata acgagctccc ttcgagttgc caacggtagc gcgcaagaag gtgagcgtgg
      181 gcttcgacgc gggctgatgt cgtcggacgc cggcgtgctg ctgctgcgcg aggtggagcg
      241 gcggctggga atcgcagcgc ggctggcggc atgcgtgccg gatcggcgcg acccggaacg
      301 catcagccac ggcctggagg aaatgctgcg gctgcggatg ttcgcgatcg cggcgggcta
      361 cgaggacgcc gacgactgcg acagcttgcg ccacgaccct gcgttcaaga tggcggttgg
      421 tcggctgccg gagagcggcg atcccctgtg ctcgcagccg acgatgtcgc ggctggagaa
      481 cacgccgagc cggatcgcga tcgcgcggat gatgctggcg atgatcgagg tgttctgcac
      541 cagctacgcg cgagccccgg ccgtgatcgt gctcgacatc gacgacacgt tggatcgcgt
      601 gcacgggcag cagcagctgg cgctgttcaa cgcgcactac aacgagcgct gcttcctgcc
      661 gatccacatc tacgagggga caagcggcaa gctggtgacg acgatcctgc gtgcgggcaa
      721 gacgccgggc ggggaagagg tgcgcacggt gctcaagcac gtgatcaagc gcatccggca
      781 gcactggccg cgcgtgtcgg tcctggtgcg cggcgacagc cattatgggc gcgacgcggc
      841 gatgacgtgg tgcgaggcca ataaagtggc ctacatcttc ggcctcgccg gcaacgcgac
      901 gctcgcctac atgacccagc cggtggccga ggagttgcgc gtgcgtcggg cggcgtacga
      961 cgccgagaag ctgcggcgct gctgcgcgct gcgttacggc gccaagagct ggccggtgca
     1021 gcgccacgtg gtcgcgcggg tcgaggcgac gcggctcggt ctcgacgtcc gctacatcgt
     1081 gacctcgctc gccggcacgc ccgagcatct ctacgagacc gtctactgcg cacgcgggca
     1141 agcggagaac tacatcaagc tgcacaaggc gcagctcgcc tccgatcgca cctcgtgccg
     1201 tgatccgcgc gccaaccagg tacggctgat cctgcacggc gcggcctatt ggctgctcta
     1261 catgctgcgc gccgcagcac ccaagcgctc gcccctacgc cgtgccgagt tcaccacgct
     1321 gcgccttcgg ctcatcaaga tcgcagcgcg cgtcgtcgag ggtgccgcgc gcatccgcgt
     1381 ctggctgccg acggtctgtc tcgatcgcgc gaccttcgcg ctgcttgccg gccgcttcgc
     1441 tgccgcagga ccgtgagccg cgggcgcgtg tgcccgtcga acccgttccg aaacccccag
     1501 ccgcagaaaa atcagaccgc cgactcgcgc cgctcgtcgt gccaccgaca ctcgcgcccg
     1561 cgcaccaaga cgaccccgcc gctgccgatc ggctcgcgtc atgaatagat cgggctagta
     1621 gccaagcgtg cccggcgccg gcccgaacac gggatcgtcc gattcacggt agaggttttg
     1681 cgtc
//
LOCUS       DATANS010000508         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_67368, whole genome shotgun sequence.
ACCESSION   DATANS010000508 DATANS010000000
VERSION     DATANS010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67368"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="VHT22_07490"
     CDS             <1..1410
                     /locus_tag="VHT22_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3137435.1"
                     /translation="PKMRSPALAVFEGKLSGIAAPIAAAKKPGARFDYDAPIEGLQAI
                     DRYTIRLKFDQSEYSLLSDLTTINTAAVAREVIEAYGDGNGWAMANPVGSGPYRLKEW
                     RRGQRITLEANPGFRDQRYPDSTDPADKSLLATMRGKKIPMIGRIEISIMEESNPRLL
                     AFQGGDLDYLQIPNELIWNVLSPDQKLKPELAKRGIRVGTGIQPAVVYTYFNMEDPVV
                     GGLTTDRIALRRAISMAYNVDEDIRIARQGQAEPSTQPLPPNVEGHDPNFQGYVRYDV
                     RGAKALLDKFGYVDRDGDGWRDLPDGKPLVLSLASPPSSSDRLYDELWQKSLAAVGLR
                     VEFVKQKWPDLYKMGRAGKLQMWTLANTSSTPDGVGFFDLLYGPNAGDQNLARFRLPE
                     FDALFARARLMPEGPERVKMFHRLSELVTAYAPWQLSVYRRETILVQPWLQGYKYNTF
                     YVHPWQYYDIDMKRRAAGG"
BASE COUNT          372 a          661 c          646 g          354 t
ORIGIN      
        1 ccgaagatgc gctcgccggc gctggcagtg ttcgagggca aattaagcgg catcgcggcg
       61 ccgatcgcgg ccgccaaaaa gcccggagcc cgattcgatt acgatgcgcc gatcgaaggc
      121 cttcaggcga tcgaccggta cacgatccgg ctcaagttcg atcaatccga gtattcgctg
      181 ttgtccgacc tgacgacgat caacaccgcc gcggtcgcgc gcgaggtcat cgaagcctac
      241 ggcgacggca atggctgggc gatggccaac ccggtcggct cggggccgta ccggttgaag
      301 gagtggcgcc gcgggcagcg catcactttg gaagcgaacc cgggctttcg cgaccagcgc
      361 tatcccgaca gcaccgatcc ggccgacaag tcgttgctcg cgaccatgcg cggcaagaaa
      421 atcccgatga tcggccggat cgaaatcagc atcatggagg aatcgaatcc gcgcctgctc
      481 gctttccagg gcggggacct cgactacctg cagataccga acgagctgat ctggaacgtg
      541 ctcagtccgg accagaagct caagcccgag ctcgccaagc gcggcatccg cgtcggcacc
      601 ggcatccagc ccgcggtcgt atacacctac ttcaacatgg aggacccggt ggtcggcggg
      661 ctgaccaccg acaggatcgc gctgcgccgc gcgatcagca tggcttacaa cgtcgacgag
      721 gacatccgca tagcgcgcca gggccaggcc gagccgtcga cccaaccgtt gccaccgaat
      781 gtcgaaggcc acgatccgaa ttttcagggc tacgttcgct acgacgtccg tggcgcaaag
      841 gcgctgctcg acaagttcgg ctacgtcgat cgcgacggcg acggctggcg cgatctgccg
      901 gacggcaagc cgctggtttt gtcgctggcc tcgccgccgt cgagcagcga ccgcctctac
      961 gacgagctgt ggcaaaagag cctcgcggcg gtgggcttgc gggtcgaatt cgtcaagcag
     1021 aagtggccgg atctgtacaa gatggggcgc gccggcaagt tgcagatgtg gacgctggcg
     1081 aatacgagct cgacgccgga tggtgtcgga ttcttcgatc tgctctacgg cccgaacgcc
     1141 ggcgatcaga atctcgcacg cttccgcctg cccgagttcg acgcgctgtt cgcccgcgcc
     1201 cgcttgatgc cggagggccc ggagcgcgtg aaaatgtttc accgtttgtc cgagctcgtt
     1261 accgcctacg cgccgtggca gctgagcgtc tatcggcgcg aaaccatcct cgtgcagccc
     1321 tggctgcagg gctacaaata caacacgttc tacgtgcatc cctggcagta ctacgacatc
     1381 gacatgaaac gcagggcggc aggcggctga ccagttgtca ttccggcgca agcggtaacc
     1441 cggtggcgcc gggccgaacg aacgcggatc cccgcgttcg cgggaaggac gtttcgactg
     1501 tgcagcggcg cagcgattcg tcgcacaaat acgacactgt tcgcggcagg cgcacgcacg
     1561 atgcgcgatc gtgattgcgc cgccgatgcg atctcgcgta cactggcgtc gacttcaacc
     1621 actcttcagg gatgatcctg ctatgaccag aacgccgacg accttcgtca ccgcgattgc
     1681 gatcgcggcg cgcgcgttcg caggagagtt tccttccggc gcgcatttcc ccggcttctg
     1741 gtcggggacg ccgctgccgc gaccttagcc cgggcgttcc cgccgggctc ggcgggaatc
     1801 gggatccggg gttttcccgc gtttcgtttc tcgccggtgg cgattgaccg gcggccgcgc
     1861 ttttacaagc cgcggccgga ggaaacgaag atgaacgata ccgacaaatt acagcactgg
     1921 tttcgccgca acttcgacgg cctttggctt tccgcgagct tccggaggct ttggctctcg
     1981 ctgaccgtca cgtcgttcgg cgcgcagatc accaatctgg cgctgccgtt gac
//
LOCUS       DATANS010000509         1949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_655870, whole genome shotgun sequence.
ACCESSION   DATANS010000509 DATANS010000000
VERSION     DATANS010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655870"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1284)
                     /locus_tag="VHT22_07495"
     CDS             complement(34..1284)
                     /locus_tag="VHT22_07495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX3137436.1"
                     /translation="MTVRASQLGALTDALAAVLPFKEPADSVLRGFFRHHRGLGQDDR
                     AFIAEGVFAALRRLASLRVQAGSSSPRRLAIAATVRELGASLREIEGALSIAETAWAK
                     EFKAAKPVLSPAEAAELPDWLWDKLTAALPAGERDALVRSWLAPAPLDLRINSMKITR
                     DQAQAQLKATGIDAEPTPYSPLGLRVAGKPALQSHPLFTSGAIEVQDEGSQLLGLLVA
                     PRRTDMVADFCAGAGGKTMLLGALMRSQGRVYAFDVSRARLARFKPRLARSGLSNVQP
                     TLIENERDTHIKRLAGKIDRVLVDAPCSGFGTLRRNPDLKWRQAAEAIPELAIKQARI
                     LAAAATLVKPGGRLVYATCSVLPDENEAIVESFVGAHPEFALGDAAAELRRAGVALDT
                     GRTLKLSTARHGCDGFFAAVLERS"
     gene            complement(1281..>1949)
                     /locus_tag="VHT22_07500"
     CDS             complement(1281..>1949)
                     /locus_tag="VHT22_07500"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX3137437.1"
                     /translation="AAPSLAPPRELPPRIDLAYRAFLGTRGFLIGDAVYRLEHSGSQY
                     RISTVGEARGLAALLFPGEARAKSEGAITADGLQPTNYSVERTNRREAASFDWESGMV
                     QLNGDQSAPLELPTFDPLVVLWQFYFSPPAQDQIEFNIATTRRIYHVTFRRIGTEKIT
                     LPFGDFDTEIWKRVDADSTVQAQIWLAPSLRYVAVKLRLSNARATVEGLLDSIRVDDT
                     VAQQ"
BASE COUNT          297 a          672 c          674 g          306 t
ORIGIN      
        1 tcgccagcgc gccggccgcg gcggttgcgg ctctcatgag cgctcgagca ctgcggcgaa
       61 gaatccgtcg cagccgtggc gcgccgtcga cagcttgagc gtgcgtccgg tatcgagcgc
      121 gacgccggcg cgccgcaatt ccgctgccgc gtcgccgagc gcgaattcgg gatgggcgcc
      181 gacgaacgat tcgacgatcg cctcgttttc gtcgggcaga acgctgcagg tcgcgtacac
      241 cagccggccg cccggcttga ccagcgttgc cgccgccgcg aggatcctcg cctgcttgat
      301 cgcgagctcg gggatcgctt ccgccgcctg tctccacttc aggtccggat tgcggcgcag
      361 cgtgccgaag ccgctgcacg gcgcatcgac cagcacccgg tcgatcttgc cggccagccg
      421 cttgatgtgc gtgtcgcgct cgttctcgat cagcgtcggc tgcacgttcg acagtcctga
      481 acgtgccagc cgcggcttga accgggcgag ccgcgcgcgc gagacgtcga acgcgtagac
      541 ccttccctgc gagcgcatca gcgcccccag cagcatggtc ttgccgccgg cgccggcgca
      601 gaagtccgcg accatgtcgg tgcgcctggg cgccaccaga agcccgagca actggctgcc
      661 ttcgtcctga acttcgatcg cgccggaagt gaacaacgga tgcgattgca acgcgggctt
      721 gccggcgacg cgaagaccca gcggcgagta cggcgtcggc tcggcgtcga tgccggtggc
      781 cttgagctgg gcttgcgcct gatcgcgcgt gatcttcatc gagttgatgc gaagatccag
      841 cggcgccggc gcgagccacg agcgcaccag cgcgtcgcgc tcgccggcgg gaagcgcggc
      901 ggtgagcttg tcccagagcc agtcgggcaa ctcggcggct tcggcgggcg agagtaccgg
      961 ctttgccgcc ttgaattcct tcgcccacgc cgtttcggcg atcgacagcg cgccttcgat
     1021 ctcgcgcagg ctcgcgccga gttcgcgcac cgtggcggcg atggcgaggc ggcgcggtga
     1081 actgctgccg gcctgcaccc ggagcgacgc cagccggcgc agcgcagcga acacgccctc
     1141 ggcgatgaac gcgcgatcgt cctgcccgag tccgcgatgg tgccgaaaaa aaccgcgcaa
     1201 cacgctgtcg gcaggttcct tgaacggcag cacggccgcc agcgcgtcgg tcagcgcgcc
     1261 gagctggctc gcgcgaacgg tcattgctgc gcgacggtat cgtcgacgcg gatgctgtcg
     1321 agcaggcctt cgacggtcgc gcgcgcgttg gacagccgca gcttgaccgc gacatagcgc
     1381 agcgacggcg ccagccatat ctgcgcctgg accgtgctgt cggcgtcgac ccgcttccag
     1441 atttcggtgt cgaagtcgcc gaacggcaac gtgatttttt cggtgccgat gcggcggaag
     1501 gtcacgtgat agatcctgcg cgtggtcgcg atgttgaact cgatctggtc ctgcgccggc
     1561 ggcgagaaat agaactgcca gagcacgacc agcggatcga acgtcggcag ctccagcggc
     1621 gccgactggt cgccgttaag ctggaccatg ccggattccc agtcgaagct ggctgcctcg
     1681 cgccggttag tccgctcgac gctgtagttg gtcggctgca atccgtcggc ggtaatcgcg
     1741 ccctcgctct tcgccctcgc ctcgccggga aacaaaagcg ccgcgagccc gcgcgcctcg
     1801 ccgacggtcg agatgcggta ctgactgccc gagtgctcga gccggtagac ggcgtcgccg
     1861 atcaggaaac cgcgcgtgcc gaggaacgcg cgatacgcaa gatcgatccg tggcggcaat
     1921 tcgcgcggcg gcgccagcga cggcgcggc
//
LOCUS       DATANS010000510         2805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_611003, whole genome shotgun sequence.
ACCESSION   DATANS010000510 DATANS010000000
VERSION     DATANS010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611003"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VHT22_07505"
     CDS             <1..661
                     /locus_tag="VHT22_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX3137438.1"
                     /translation="MYEFDNGLALIELSDAQKLYRLNDAVSGVRLKLDDMFAAPAVAR
                     ELAQVMPVDAEVRDWTMDHANFFRAVAIEKRVMFIILTLIVAVAAFNIVSAQVMVVTD
                     KQADIAILRTLGASPASILTIFVIQGALVGFIGTLVGVVGGIALALNIGVVVPFIERT
                     FSIQFLDKSVYYISEVPSDLQRADIVLIAAIALLLALLATLYPSWRAAKVNPAEALRY
                     E"
     gene            654..1352
                     /gene="lolD"
                     /locus_tag="VHT22_07510"
     CDS             654..1352
                     /gene="lolD"
                     /locus_tag="VHT22_07510"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEX3137439.1"
                     /translation="MSKVGTAPVLECRGLRKTYRSGPLDVPVLLGIDLAVAPGERLAI
                     VGASGSGKSTLLHLLGGLDAPSAGEVLVQGAPFAQLSEAERGRVRNRALGFIYQFHHL
                     LPEFSALENVAMPLYIRRDARPAARASAAALLERVGLAHRAQHRPAELSGGERQRVAL
                     ARALVTAPACVLADEPTGNLDRRTAGGVFDLMLELNRSAGTSLVIVTHDPDLAARTDR
                     TLHLLDGVLTSSED"
     gene            complement(1416..2435)
                     /locus_tag="VHT22_07515"
     CDS             complement(1416..2435)
                     /locus_tag="VHT22_07515"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX3137440.1"
                     /translation="MDDASAAPYAGLTPDCVLDALDAVGLFCDGRLLALNSYENRVYQ
                     AWLDERASGVDTVSSVVVKFYRPSRWSDEQIGEEHAFVSELAVREIPVVAPIACGGAT
                     LHRFAGFRYAVYPQCGGRAPELEDRATLEWIGRFVGRIHSVGGTAPFRVRPSLDIASF
                     GDEPREWLLANGFIPPDLVPAWESVAAQAIERVRHCYERAGAVRTIRLHGDCHAGNVL
                     WRTGGPMQGPHFVDFDDCRSGPAVQDLWMLLSGDRDDMRRQLRAVLAGYEDFCDFDDR
                     ELHLIEALRTLRLIHYSAWLARRWHDPAFPAAFPWFNTQRYWQDRVLELREQIAAMDE
                     PQLSI"
     gene            complement(2504..>2805)
                     /locus_tag="VHT22_07520"
     CDS             complement(2504..>2805)
                     /locus_tag="VHT22_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX3137441.1"
                     /translation="KLDPKRWRKLDDESVIAQLIDVKGIGRWTAEMFLMFHELRPDVL
                     PVDDLGLQKAMARLFLKDARPTRDAMLALAEPWRPWRSVATWYLWRSLEPVPVEY"
BASE COUNT          479 a          942 c          926 g          458 t
ORIGIN      
        1 aatgtacgag ttcgacaacg gtctcgcgct gatcgagctg tccgatgcgc agaagctgta
       61 ccgcctcaac gacgcagtca gcggcgtgcg gctcaagctg gacgatatgt tcgcggcacc
      121 cgcggtcgcg cgcgagctcg cgcaagtcat gccggtcgac gcggaagtgc gcgactggac
      181 gatggatcac gccaatttct ttcgcgccgt cgcgatcgag aaacgagtga tgttcatcat
      241 ccttacgctg atcgtcgcgg tcgctgcatt caatatcgtt tccgcacagg tgatggtggt
      301 caccgacaag caggccgaca tcgccatcct gcgcacgctg ggcgcgtcgc cggcgagcat
      361 cctgacgata ttcgtcatcc agggcgcgct ggtcggcttc atcggaacgc tggtaggcgt
      421 ggtcggcggg atcgcactgg cgctcaacat cggcgtcgtc gtgccgttca tcgagcgcac
      481 gttcagcatc cagttcctgg acaagagcgt ctattacatc agcgaggtgc cgtccgacct
      541 gcagcgcgca gatatcgtgc tgatcgccgc gatcgcgttg ctgctcgcgc tgctcgcgac
      601 gctgtatccc agctggcggg cggccaaggt caatcccgcg gaggcgctgc gctatgagta
      661 aggtcggcac agcgcccgtg ctcgagtgcc ggggcctgcg gaagacgtat cgcagcggac
      721 cgctcgacgt gccggtgctg ctgggcatcg atctggcggt ggccccgggt gagcgcctgg
      781 cgatcgtcgg cgcgtcgggc tcgggcaaga gcacgctttt gcatctgctc ggcgggctgg
      841 acgcgcctag cgccggcgaa gtgctcgtgc aaggcgcacc gttcgcgcag ttgtccgaag
      901 ccgagcgcgg ccgggtgcgc aatcgcgcgc tgggctttat ctaccagttt catcatctgc
      961 tgccggaatt ctcggcgctt gaaaacgtcg caatgccgct ctacatccgc cgcgatgcga
     1021 gaccggcggc acgggcaagt gccgccgcgc tgctggagcg tgtcggcctg gctcatcgcg
     1081 cgcagcatcg tccggccgag ctctcgggcg gcgaacgaca gcgcgtcgcg ctggcacgcg
     1141 ctctggttac ggcaccggcg tgtgtgctcg ccgacgagcc gaccgggaac ctcgaccggc
     1201 gcaccgccgg aggcgtgttc gatctgatgc tcgagctcaa tcgcagcgca ggaacgagcc
     1261 tcgtgatcgt gacgcatgat cccgatctgg cggcgcgcac cgatcgaacc ttgcacctgc
     1321 tcgacggcgt gctgacgtcg tcggaggact aagcccgcgc gccgggccgg gtaggacacg
     1381 cacggcccag cggcgtcctg ccggatgcct tcccgctaga tcgacaattg cggctcgtcc
     1441 attgccgcga tctgctcgcg caattccagc acgcgatcct gccagtagcg ctgggtgttg
     1501 aaccagggga acgccgccgg gaaagcggga tcgtgccagc ggcgcgccag ccatgccgaa
     1561 taatggatca gccgcagcgt gcgcagcgct tcgatcagat gcagctcgcg gtcgtcgaag
     1621 tcgcagaaat cctcgtaccc cgccagcacg gcccgcaact ggcgccgcat gtcgtcgcgg
     1681 tcgcccgaca gcagcatcca caaatcctgc accgcgggac cgctgcggca atcgtcgaaa
     1741 tcgacgaaat gcggaccttg catcggcccg ccggtgcgcc acagcacatt gccggcatgg
     1801 cagtcgccgt gcaggcgaat agtgcggact gcgcccgcgc gctcgtagca atgccgcacg
     1861 cgttcgatcg cctgcgcggc gacgctctcc catgcgggga ccaggtcggg cgggatgaaa
     1921 ccgttcgcca gcagccactc gcgcggctcg tcgccgaagc tcgcgatgtc gagcgacggc
     1981 cgcacgcgaa acggcgcggt cccgcccacg ctgtggatgc gcccgacgaa acggccgatc
     2041 cattcgagcg tcgcacgatc ctcgagctcg ggagcgcggc cgccgcattg cggatagacc
     2101 gcatagcgga agcccgcgaa acgatgcagg gtcgcgccac cgcatgcaat cggcgcgacg
     2161 accggaatct cgcgcaccgc cagttcggag acgaatgcgt gctcctcgcc gatctgctca
     2221 tcgctccacc gcgacggacg atagaacttg acgacgaccg aagacaccgt atccacgccg
     2281 ctggcgcgct cgtcgagcca tgcctggtag acgcgattct cgtagctgtt cagcgcaagc
     2341 agcctgccgt cgcagaagag tccgacggca tcgagcgcat cgagcacgca gtcgggcgtg
     2401 aggcccgcat aaggtgcagc cgaagcatcg tccatgcagg cattgtacgt agggccgcgg
     2461 cgactactgc cggcgccacg cgccaggagt tcgcgccgcc gggctaatac tcgactggaa
     2521 ccggctcgag gctgcgccac aagtaccagg tggcgaccga gcgccatggc cgccagggct
     2581 cggccagcgc aagcatcgcg tcgcgggtcg ggcgcgcatc cttcagaaat agtcgtgcca
     2641 tcgccttttg caggccgagg tcgtcgaccg gcagcacatc ggggcgcagc tcgtgaaaca
     2701 tcaggaacat ctccgccgtc cagcgcccga ttcccttgac gtcgatcagc tgtgcgatta
     2761 ccgactcgtc gtcgagcttg cgccagcgtt tcggatcgag cttgc
//
LOCUS       DATANS010000511         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_571885, whole genome shotgun sequence.
ACCESSION   DATANS010000511 DATANS010000000
VERSION     DATANS010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571885"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..597
                     /gene="msrB"
                     /locus_tag="VHT22_07525"
     CDS             142..597
                     /gene="msrB"
                     /locus_tag="VHT22_07525"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX3137442.1"
                     /translation="MTRRVHGDAALAAAEKTGSFEVAKTDAEWKAQLTPAQYDVLRKE
                     GTERPFTSPLNDEKRRGLFVCAGCALPHFTSEMKFDSGTGWPSFFTSIPGHLATRQDY
                     LLIVPRTEYHCVRCGGHQGHVFDDGPPPTGQRWCNNGVAQRFIPATGKA"
     gene            complement(572..2119)
                     /locus_tag="VHT22_07530"
     CDS             complement(572..2119)
                     /locus_tag="VHT22_07530"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX3137443.1"
                     /translation="MTKLAFSARQRGAALLMAAMLTVSPIAAAQQNLPELGDPSLVAM
                     SAPQERKLGENVMRNVRSSGAYLNDPEVNGYLNELGNRLVGAIPGAPFDFEFFGIADP
                     AINAFALPGGFIAVHTGLILLAQSESELASVLGHEITHVTQHHIARSIGNQRDTMLLT
                     IAALAAAVLASQSRSSSSGDLTQAAIAGAQGLAIQSQINFTRQNEEEADRLGFERTYA
                     AGFDPSAMATFFGRLQRAATWFEGDSPSYLRDHPETYQRIAEAQARSFTKPFRQVPDS
                     LDFHMVRALLKSYEGTPRDAVFFFDDALAEKKYNNEIAAHYGLVASLLRAQNFKRAQT
                     ELATLEKLSPPHPMIDAMAGQVLMQSGQLVPAISRYETALARYPEKKQLIYDYPEALL
                     KNDQTAKAATFVSRQLDRYPSDGQLHQIAARIYAALGKPMLEHQHQAEYYAWLGNLQG
                     AIIQFELATKARDADFFQSSVVETRLRELRNELEEQKKAAAKSKPGLARLGSAACGPV
                     MPCRSPE"
     gene            2166..>2345
                     /locus_tag="VHT22_07535"
     CDS             2166..>2345
                     /locus_tag="VHT22_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX3137444.1"
                     /translation="MAETRAQFTHFDAAGQAQMVDVGGKDVSRRVARAAGRILMRPET
                     LALIREGKASKGDVLG"
BASE COUNT          400 a          739 c          797 g          409 t
ORIGIN      
        1 tggcgcaaca ccgaggacgc cgacgtgggg cgcttcctgc gcttcttcac cctgctgtcg
       61 cgcggggaga tcgagtcgtt ggagacgtcg accgccgcgg cgccggagcg gcgcgccggc
      121 cagctggccc tcgcccgcga cgtgacgcgc cgcgtccacg gcgacgcggc gctcgccgcg
      181 gcggagaaga cggggtcgtt tgaagtcgcc aagaccgacg ccgaatggaa ggcgcagctg
      241 acgccggcgc aatatgacgt gctgcgcaag gaaggcaccg agcgcccttt caccagcccg
      301 ctgaacgatg aaaagcggcg cggccttttt gtctgcgccg ggtgcgcgct cccgcatttc
      361 acgtcggaaa tgaagttcga cagcggcacg ggctggccga gctttttcac cagcatcccg
      421 ggacatctgg cgacccggca ggattacctg ctgatcgtcc cgcggaccga gtaccactgc
      481 gtgcgctgcg gcggacacca gggtcacgta ttcgacgacg gcccgccgcc caccggtcag
      541 cgctggtgca acaacggcgt cgcgcagcgc tttattccgg cgaccggcaa ggcataaccg
      601 gtccgcaggc agcggagccg agccgggcca gccccggctt cgacttcgcc gccgcctttt
      661 tctgctcctc cagctcgttg cgcaactcgc gcaggcgggt ttcgacgacc gacgactgaa
      721 agaagtcggc atcgcgcgcc ttggtcgcca gctcgaactg gataatcgcg ccctgcagat
      781 tgcccagcca tgcgtagtac tcggcttgat gctgatgctc gagcattggc ttgccgagcg
      841 ccgcgtagat gcgggcggcg atctggtgca gctgtccgtc ggacgggtaa cggtccagct
      901 gccgagacac gaacgtggcg gccttggccg tctgatcgtt cttcagaagc gcctccggat
      961 agtcgtagat cagctgcttt ttctccgggt agcgggcaag cgcggtttcg taccggctga
     1021 tcgccgggac cagctgtcct gactgcatca gcacctggcc cgccatcgcg tcgatcatcg
     1081 ggtgcggcgg cgagagcttc tcgagggttg cgagctcggt ctgcgctcgc ttgaagttct
     1141 gcgcgcgcag cagggaggcg accagtccgt agtgcgccgc gatctcgttg ttgtattttt
     1201 tctcggcgag cgcgtcgtcg aagaagaaga ccgcatcacg cggcgtgccc tcgtagcttt
     1261 tcagcagggc gcgcaccatg tggaagtcaa gcgaatcggg gacctggcgg aacggcttgg
     1321 tgaacgagcg cgcctgcgct tcagcgatgc gctggtacgt ctcggggtga tcgcgcaggt
     1381 atgagggcga gtcgccctcg aaccaggttg cagcccgttg cagccttccg aaaaaggtcg
     1441 ccatcgcgct cggatcgaag ccagctgcat aagtccgctc gaaccccagg cggtcggctt
     1501 cctcctcgtt ctggcgggtg aaattgatct ggctctggat cgccaggcct tgcgcgccgg
     1561 cgatcgcggc ctgggtcagg tcgccgctgg acgaggaacg ggactgcgag gccaataccg
     1621 cggcggcgag cgcggcaatc gtgagcagca tcgtatcgcg ctggttgccg atgctgcgcg
     1681 cgatatggtg ttgcgtgacg tgcgtgattt cgtggccgag taccgacgcc agctcggatt
     1741 cggactgcgc cagcaggatg agcccggtat gaacggcgat gaagccgccc ggcagcgcga
     1801 acgcattgat cgccggatcg gcgatgccaa agaactcgaa gtcgaacggc gcgccgggaa
     1861 tcgcgccgac aaggcgattg cccaactcgt taaggtaacc attgacttcg ggatcgttca
     1921 ggtacgcgcc tgaactgcgg acattgcgca tcacgttctc gccgagcttg cgctcctgcg
     1981 gggcggacat cgccaccaac gacggatcac ccagctctgg cagattctgc tgggccgctg
     2041 cgatcggcga caccgtcagc atcgcagcca tcagcagtgc ggcgccgcgc tgccgggcgg
     2101 aaaaggcgag cttggtcatg gtgctatgat tgccacggat cgcgcaaagt ttcaggagca
     2161 ggccgatggc ggagaccaga gcgcagttta cccattttga cgccgcgggt caggcacaaa
     2221 tggttgatgt cggcggcaag gacgtaagca ggcgtgtcgc ccgcgccgcc ggccggattc
     2281 tgatgcggcc tgaaacgctg gcgctcattc gcgaaggcaa ggccagcaaa ggcgacgttc
     2341 tcggc
//
LOCUS       DATANS010000512         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_24837, whole genome shotgun sequence.
ACCESSION   DATANS010000512 DATANS010000000
VERSION     DATANS010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24837"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1155)
                     /gene="pyrC"
                     /locus_tag="VHT22_07540"
     CDS             complement(118..1155)
                     /gene="pyrC"
                     /locus_tag="VHT22_07540"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX3137445.1"
                     /translation="MPDMLTITRPDDWHLHVRDGAPMRSVVGTTARVFGRAVLMPNLD
                     PPVTTVAAAGAYRDRVVAALPPGSRFTPLLTLYLTDDTSPVEIERARAAGFVIGVKYY
                     PAGATTNSASGVTELARVYPALAAMEKCGLPLLVHGEVTDPDIDVFDRERVCVERVLS
                     RIVRDFPALRLVVEHITTREAAQFVAAAPAHVGATITPQHLLWSRNALFAGGLRPHAY
                     CLPVLKREAHRRALVDAATGGDAKYFLGTDSAPHARDAKESACGCAGCFSAPFAIELY
                     AEVFDAAGKLDKLEGFASFHGADFYGLPRNAGTITLERAPWTVPASYPFGAGTVVPVF
                     AGETLRWRVTD"
     gene            1506..1865
                     /gene="dksA"
                     /locus_tag="VHT22_07545"
     CDS             1506..1865
                     /gene="dksA"
                     /locus_tag="VHT22_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEX3137446.1"
                     /translation="MSEEQLAFFRQRLLELRAQLLNNADDTGQHLRENEVTTDPSDRA
                     TLEEEYTLELRTRDRERKLLKKIEKSLKMIDDGTYGYCEETGEPIGIPRLLARPTATL
                     SLEAQERRERVQKLYGD"
BASE COUNT          383 a          626 c          683 g          313 t
ORIGIN      
        1 cccagcatcc tgctctgcgg agcccggact ttcctctgcc agcttcgggc ttgcgccgtt
       61 agcggacagc gacggtctgg ccgacttcga tggtcagttt agcacgccga aaacgcatta
      121 gtcggtgaca cgccagcgca gggtctcccc ggcgaagacc ggcacgaccg tcccggcgcc
      181 gaacggatag ctcgccggca ccgtccaggg cgcgcgctcg agcgtgatcg tgccggcatt
      241 gcgcggcagg ccgtagaaat ccgcaccgtg aaaactggcg aagccctcga gcttgtcgag
      301 cttgcctgcg gcatcgaaca cctcggcata gagctcgatc gcgaacggcg ccgaaaaaca
      361 tccggcacag ccgcacgcgc tctcctttgc atccctcgcg tggggagcgg aatcggtgcc
      421 gagaaaatat ttcgcatcgc cgccggtcgc cgcatcgacc agcgcgcggc gatgcgcttc
      481 acgtttcagc accggcagac aataggcgtg cgggcgcagt ccgccggcga atagcgcatt
      541 gcgcgaccac aacaagtgct gcggcgtgat ggtcgcgccg acatgcgccg gcgcggcggc
      601 gacgaactgg gctgcttcgc gcgtcgtgat gtgctcgaca accagcctca aggccgggaa
      661 gtcgcgcacg atgcgcgaga gcacccgctc gacgcagact cgttcgcgat cgaacacatc
      721 gatatccggg tcggtgactt cgccatgcac gagcagcggc agtccgcact tttccatcgc
      781 agcaagcgcc ggatagacgc gcgcgagctc ggtaaccccg gatgccgaat tggtcgtcgc
      841 gccggctgga tagtatttga cgccgataac gaaaccggca gctcgagcgc gctcgatttc
      901 gaccggactg gtgtcgtcgg tgaggtacag cgtcaaaagc ggcgtgaaac gcgatcccgg
      961 cggcagcgcc gcgacgactc tgtcgcggta ggcgcccgcg gcggcgacgg tggtgacggg
     1021 gggatcaaga ttcggcatca gcaccgcgcg accgaatacg cgggccgttg tgccaacgac
     1081 cgagcgcatc ggcgcaccat cgcgcacgtg gaggtgccag tcgtcggggc gggtgatcgt
     1141 cagcatgtca ggcattgttt ttgcatgcat tatacggggc tgcccgtccg ccggtcgggt
     1201 tgccgaaccg tgcgaattga ccttggcttg cagcgtcggg ttcgcgatat aatgcgcggt
     1261 ttagtgcggt ggctcatcgg agcttgatgc gtccccgtgc ttctcacggg gccctgcctc
     1321 cggcaaagcc gccggtggga gcgtctggat gggcacgccg gccaaagctt ggcgggctcg
     1381 tcgtgcgaag cgatcaggtg cgggcatacg gcggggaagg tggaaacgtg gcgacaaaaa
     1441 acgtagcgaa aaaggtcagg gtgctgaccg agccagatct cctcaaggcg agcgacaagg
     1501 agtacatgag cgaggagcaa ctggcgttct tccgccagcg gctactcgag cttcgcgcgc
     1561 aactgctgaa caatgccgat gacaccggcc agcacctgcg cgaaaacgag gtgacgaccg
     1621 acccgtcgga tcgcgcgacg ctcgaagagg aatacacgct ggagctgcgc acccgggatc
     1681 gggagcgcaa gttgctcaag aagatagaaa agtcgctgaa gatgatcgac gacgggacct
     1741 acggctattg cgaggagacc ggcgagccga tcgggattcc gcggcttctg gcccggccga
     1801 cggcgacctt gtcgctcgag gcgcaggagc gccgcgagcg cgtgcaaaag ctctacggcg
     1861 attgagctcg agcattgcgt cggcggcggc gggcagtgcg cccgtgccgc ggcgacgggg
     1921 aggtccggcc gcagcgttcc cgccgcggcc ggtaaattct acaaaccgaa aattgcgcgg
     1981 tgcgaccacc ctcccgccat aaaga
//
LOCUS       DATANS010000513         3467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_24933, whole genome shotgun sequence.
ACCESSION   DATANS010000513 DATANS010000000
VERSION     DATANS010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24933"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1021)
                     /gene="rpoS"
                     /locus_tag="VHT22_07550"
     CDS             complement(68..1021)
                     /gene="rpoS"
                     /locus_tag="VHT22_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HEX3137447.1"
                     /translation="MRAHSAADDDESPAAPEPDTQTRDLPLDMPVVGVGADFLNDVTQ
                     IYLNEIGQSPLLTPAEELEYARANRDGDFEARQKMIEHNLRLVVSIAKHYLNRGLTLA
                     DLIEEGNLGLIHALEKFDPERGFRFTTYATWWIRQSIERAIMNQSRTIRLPAHVVKEL
                     NIVLRAMRHLETHGMLGQGRDPTMDDVAHLLGKPVEQVRKVLGYNEHVTSLDAAYDVD
                     SGLSIGDQLSDEDAPGPELLLHNSEIESWVKQWLDELSERQRLVIERRYGLNGCDVAT
                     LEHLARELGVTRERVRQIQAEALEKLRARLRRKGLSRDALL"
     gene            complement(1040..2035)
                     /locus_tag="VHT22_07555"
     CDS             complement(1040..2035)
                     /locus_tag="VHT22_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX3137448.1"
                     /translation="MTRPPAPVSERAAPPPETIEGAPPAAASAPPPEAPPPTHTVKRG
                     ETLYQIALDHGLDYRELAAWNNIENVNVIRVGRVLVLAPPGEAGAAVAAPGAAVTTPL
                     PAAPAIAPVGSETRTVPFTGGAPSGRGNTAAYKSEPKALKVPYSEQALAQMQQSSAAA
                     PPAPPPAAPPIVAPSAAASPATAPAATPGTVAAAPARTESAHVPEIDGGNIDWAWPAA
                     GKVISGFSETANLKGIDIAGKSGQPVLASGPGKIVYAGSGLRGYGKLIIIKHNNTFLS
                     AYAHNREILVKEGQQVARGQKIAEMGDSDADQVKLHFEIRRFGKPVDPAKFLPPA"
     gene            complement(2107..2790)
                     /locus_tag="VHT22_07560"
     CDS             complement(2107..2790)
                     /locus_tag="VHT22_07560"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX3137449.1"
                     /translation="MSAGVEKFGGIGMTSARTRTRMIERLREQGIRDEAVISAMTAVP
                     RHIFVEEALAIRAYDDSPLPIGAGQTISQPYVVARMTELLRAGGHLHRVLEIGTGCGY
                     QTAVLAHLADEVYTVERIGSLVAKARQNLRALKLNNVRIKHGDGSADLGEELEVDGII
                     VTAAATQVPTALLRYLKIGGRMVLPLAIHDDDGRGAQRLTVIEKAPAGIREQSFDAVR
                     FVPLLPGLA"
     gene            complement(2852..>3467)
                     /gene="surE"
                     /locus_tag="VHT22_07565"
     CDS             complement(2852..>3467)
                     /gene="surE"
                     /locus_tag="VHT22_07565"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX3137450.1"
                     /translation="NSLTLDRPLTVRRAPSGFLFVNGTPTDCVHLAVTGLLDELPDMV
                     ISGINHGANMGDDTIYSGTVAAATEGYLLGIPSIAVSLTSKTGHHFETAAKVVADLIS
                     RHRRKPSGAWLLNVNVPDVPVTDIKGVRVTRLGKRHKAESVVKTETPRGETCYWVGAA
                     GAAADAGEGTDFHAVGLGFVSVTPLQVDLTNHAEMPRIAEWIEA"
BASE COUNT          525 a         1144 c         1169 g          629 t
ORIGIN      
        1 tgcccgcctc cagggtcgca tccccgcgcg cttcgcgcgc cgggcccctg ctccgccttc
       61 cggcgtgcta aagcagcgcg tcgcgcgaca agcccttgcg acgcaacctc gcccgtagct
      121 tttcgagcgc ctcggcctgg atctggcgca cgcgctcgcg agtgacgccc agctcgcggg
      181 cgagatgctc gagcgtcgcg acgtcgcagc cattcaatcc ataacggcgc tcgatcacca
      241 ggcgctgccg ctcggagagc tcgtccagcc attgcttgac ccagctttcg atctcggaat
      301 tgtgcagcag cagctcgggg cccggcgcat cctcgtcgct caactggtcg ccgatcgaca
      361 ggccggagtc gacatcgtag gccgcatcga gcgaggtgac gtgctcgttg tagcccagga
      421 ctttgcgcac ctgctcgacc ggcttgccga gcagatgcgc gacgtcgtcc atcgtcggat
      481 cgcgtccctg gccgagcatg ccgtgcgttt cgaggtggcg catcgcgcga agtacgatat
      541 tgagctcctt gaccacgtgc gccggcagcc ggatcgtccg cgactggttc atgatcgcgc
      601 gctcgatcga ctggcgtatc caccaggtcg cgtaggtcgt gaagcggaag ccgcgctccg
      661 gatcgaactt ctccagcgcg tgaatcaggc cgagattgcc ttcctcgatc agatcggcaa
      721 gcgtcaggcc gcgattcaga taatgcttgg cgatgctgac caccagccgc aggttgtgct
      781 cgatcatctt ctgccgcgct tcgaagtcgc cgtcccggtt ggcgcgcgcg tactcgagct
      841 cctcggcggg cgtcagcagc gggctctggc cgatctcgtt caggtagatc tgggtgacgt
      901 cgttgaggaa gtcggcgccg acgccgacga ccggcatgtc cagcggcaaa tcgcgcgtct
      961 gggtatcggg ctccggtgcc gcgggcgact cgtcgtcatc tgccgccgaa tgcgctctca
     1021 tgcgtggccg gacccgccgc taagcgggcg gcaggaattt cgccgggtcg accggcttgc
     1081 cgaagcgacg gatttcgaag tggagcttca cctggtcggc gtcgctgtcg cccatttccg
     1141 cgatcttctg gcccctcgcg acttgctggc cttccttgac caggatttcg cgattgtgcg
     1201 cgtaggccga cagaaacgta ttgttgtgct tgatgatgat cagcttgcca tagccgcgca
     1261 agccgctgcc cgcgtagacg atcttgcccg gaccgctggc aagcaccggc tgaccggatt
     1321 tgccggcgat gtcgatgcct ttgaggttcg ccgtttcgga aaagccggaa atgactttgc
     1381 cagctgcggg ccacgcccaa tcgatgttgc cgccgtcgat ttcgggcacg tgcgcggatt
     1441 cggtgcgcgc cggcgctgcc gcgacggtgc ctggcgtcgc ggcaggagcg gttgcgggcg
     1501 acgcggcggc agatggcgcg acgatcggtg gcgcggccgg cggcggcgcg ggaggcgcgg
     1561 ctgcgctgct ctgctgcatt tgcgccagcg cctgctccga atagggcacc ttcaaggcct
     1621 tcggctcgct tttgtacgcc gcggtgttgc cgcggccgga aggcgccccc ccggtgaacg
     1681 gtacggtccg cgtttcgctc ccgaccgggg cgattgctgg cgcggccggg agcggcgtgg
     1741 tcacggcagc gcccggcgct gcgaccgcgg caccggcttc acccggcggc gcgagcacga
     1801 gcacgcggcc gacgcggatg acgttgacgt tttcgatgtt gttccatgcg gcaagctcgc
     1861 ggtaatcgag cccgtggtcg agtgcgatct ggtagagggt ctcgccgcgt ttgacggtat
     1921 gggtcggcgg cggagcttcc ggcggcggcg ccgatgccgc ggcgggtggc gcaccctcga
     1981 tcgtttcagg cggcggcgcg gcgcgctcgc tgaccggcgc cggtggacgg gtcatgcatg
     2041 atacgagcag cagtgcggct gcaatgggcg caagcacgcg gcatgaatgc gcgaaaccga
     2101 agtgagtcat gcgagtccgg gaagcaatgg tacgaatctt accgcgtcga agctttgctc
     2161 gcggatgccg gccggcgcct tttcgataac ggtcagtcgc tgcgctccgc gcccgtcgtc
     2221 gtcatggatg gcgagcggca gcaccatgcg accgccgatt ttcaaatagc gcagcaatgc
     2281 ggtcgggacc tgcgttgccg cggcggtgac gatgatcccg tcgacttcga gctcctcgcc
     2341 cagatccgcc gagccgtcac catgtttgat gcgcacgttg ttcagcttca gcgcgcgcag
     2401 gttctgccga gccttggcga ccagcgagcc gatgcgctcg accgtgtaga cctcgtcggc
     2461 caggtgcgcc agcaccgccg tctggtagcc gcaaccggtg cctatctcca gcacgcgatg
     2521 cagatggccg ccggcccgca acagctcggt catgcgagcg acgacatacg gctgcgaaat
     2581 ggtctgtccg gcgccgatcg gcaaaggact gtcgtcgtag gcgcggatcg ccagcgcttc
     2641 ctcgacgaaa atgtggcgcg gcaccgcggt catcgctgaa atcacggctt cgtcgcgaat
     2701 gccctgttcg cgcaggcgct cgatcatgcg cgtgcgcgtg cgcgccgaag tcatgcctat
     2761 gccgccgaat ttttccacgc cggcgctcat cgcttcgccc gtcccgcgag cccgccgacc
     2821 cgatgcgaag acgatcgacc gtaacacggc atcatgcttc gatccattcg gcgatacgcg
     2881 gcatttcggc gtggttggtc aggtcgacct gcagcggcgt caccgaaacg aaacccagtc
     2941 cgaccgcatg gaaatcggtt ccttcgccgg catcggcggc agcgcccgcc gcgccgaccc
     3001 agtagcaggt ctcgccgcgc ggcgtctcgg tcttcaccac cgattcggcc ttgtgtcgct
     3061 tgcccagacg tgtgacacgc acacccttga tgtcggtgac cgggacgtcg ggcacgttga
     3121 cgttgagaag ccacgccccc gacggcttgc ggcgatgtcg cgagatgaga tcggcgacga
     3181 ccttggccgc ggtctcgaag tggtggccgg tcttgctggt cagcgacacc gcaatcgaag
     3241 gaatgcccag cagataaccc tcggtcgcgg cggccacggt gcccgagtag atcgtgtcgt
     3301 cgcccatgtt ggcgccgtga ttgatgcccg agatcaccat gtccggcagt tcgtccagca
     3361 gcccggttac cgccaggtga acgcaatcgg tgggcgtgcc gttgacgaac aggaaaccgc
     3421 tgggcgcgcg gcgcaccgtc agcggccgat cgagcgtcag ggaattg
//
LOCUS       DATANS010000514         3437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_447441, whole genome shotgun sequence.
ACCESSION   DATANS010000514 DATANS010000000
VERSION     DATANS010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447441"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /gene="pheA"
                     /locus_tag="VHT22_07570"
     CDS             complement(<1..365)
                     /gene="pheA"
                     /locus_tag="VHT22_07570"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX3137451.1"
                     /translation="MNNDDSEQEFQRHRAAIDGLDREILRVLNQRATHAQAIGKLKGE
                     SAAYRPEREAQVLRAMRDANPGPLPGDAVAGIFRQVMSACLALERRLRIAYLGPAGTF
                     SHGAVARHFGEFVDAEPCAS"
     gene            complement(433..3000)
                     /gene="gyrA"
                     /locus_tag="VHT22_07575"
     CDS             complement(433..3000)
                     /gene="gyrA"
                     /locus_tag="VHT22_07575"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX3137452.1"
                     /translation="MEQFAKETLPVSLEAEMRHSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMHESNTVWNRPYVKCARVVGDVLGKYHPHGDSATYEALVRMVQDFSLRYPL
                     IDGQGNFGSVDGDAPAAYRYTECRLEKITAEMLADIDKETVDFAPNYDGKELEPTVLP
                     TRIPNLLVNGSSGIAVGMATNIPPHNLTEIITACLTLLDNPDTTVDELIEHVPAPDFP
                     TAGIIFGTEGVREGYRTGRGRVVIRARTHIEDLEKGGRQAIIVDELPYQVNKATLLTR
                     IGELVREKKLDGISDLRDESDKSGMRVVIELKRGEVTEIILNNLYKLTQLQDSFGMNM
                     VALVDGQPRLLNLKQFLEFFLQHRREIVVRRTRFELRKARDRGHILEGLAVALSNVDE
                     IIALIKAAATPAEAKAALMARTWRSALVEEMLNRSAADAARPEGLAPEYGWQRHGSPQ
                     GYRLSEAQAQAILELRLQRLTGLEQEKITGEYREVMVQIVDLLDVLAKPARVTTVIGD
                     ELKAIRDQFGDKRRSEIVAHSADLSIEDLIAPEEMVVTLSHGGYMKAQPVAEYRAQRR
                     GGRGKQATGTKEDDFIDHLFIANTHDFILCFSNRGRVYWLKVYNVPQGGRISRGKPIV
                     NLVPLLEHEKITAILPVKEFVSDRFVFMATTKGTVKKTPLSDFSRPRPSGIIAVDLDD
                     GDFLVGAALTDGKHDVMLFSSGGKAVRFEEGDVRSMGRTAHGVRGMMLDKGQRVISML
                     VAEDEQQSVLTATENGYGKRTPIVEYTRHGRGTKGMIAIQQTARNGKVVAAALVLPDD
                     EVMLISTGGVLIRTKVSSIREMGRSTQGVTLINLGEGEKLAGLERVVETELVSDEPE"
BASE COUNT          578 a         1120 c         1059 g          680 t
ORIGIN      
        1 gaggcgcagg gctcggcatc gacgaactcg ccgaaatgcc tggcgacagc gccgtggctg
       61 aaggtgccgg ccggaccaag gtaagcgatc cgaagccggc gctccagcgc gagacaggcg
      121 gacatcacct ggcggaatat ccccgcgacc gcatcgcccg gcagcggccc cggattggca
      181 tcgcgcatcg cgcgcaacac ctgcgcctcg cgctccggac gataggcggc gctttcgccc
      241 ttgagcttgc cgatcgcctg cgcatgcgtg gcgcgctggt tgagtacgcg caggatctcg
      301 cggtcgaggc cgtctatcgc cgcgcggtgg cgctggaact cctgctcgct gtcgtcgttg
      361 ttcatcgcct gcccgggtcg tcgcccattt cggcgccggc agcgcgccgg cgactgtcgc
      421 cctgttgccg atttattccg gctcgtcgct gacgagctcg gtttcgacga ctcgctcgag
      481 gccggcgagc ttttcgccct cacccaggtt gatcagcgtc acaccctgtg tcgagcggcc
      541 catctcccgg atcgacgaaa ccttggtccg aatcagcacg ccgccggtcg agatcagcat
      601 cacctcgtcg tcgggcaaga ccagcgccgc agcgacgacc ttgccgttgc gcgcggtctg
      661 ctggatggcg atcatgccct tggtgccgcg gccatgtcgc gtgtactcga cgatcggcgt
      721 gcgcttgccg tagccgttct ccgtcgccgt cagcaccgat tgctgctcgt cctcggcgac
      781 cagcatcgag atgacgcgct ggcccttgtc cagcatcatt ccgcgcacgc cgtgcgcggt
      841 ccgccccatc gagcgcacgt cgccttcctc gaagcgcact gccttgccgc ccgagctgaa
      901 cagcatgacg tcgtgcttgc cgtcggtcag cgctgcgccg accaggaagt cgccgtcgtc
      961 cagatcgacc gcgatgatgc ccgacgggcg cggccgcgag aaatcggaca gcggcgtttt
     1021 cttgaccgtg cccttggtcg tcgccatgaa tacgaaccga tcgctgacga actccttgac
     1081 cgggaggatc gcagtgatct tttcgtgctc cagcagcggc accagattga cgatcggctt
     1141 gccgcgcgaa atgcggccgc cctgtggaac gttgtacacc ttcagccagt acacccgtcc
     1201 ccggttcgaa aagcacaaga tgaaatcgtg cgtgttggcg atgaacaggt ggtcgatgaa
     1261 atcgtcttcc ttggttccgg tcgcctgctt gccccggccg ccgcggcgct gcgcgcgata
     1321 ctcggcgacc ggctgcgcct tcatgtagcc gccgtgcgac agcgtgacca ccatctcttc
     1381 gggcgcgata aggtcctcga tcgaaaggtc ggcgctgtgc gcgacgatct cggatcgcct
     1441 cttgtcgccg aactgatcgc ggatcgcctt gagctcgtcg ccgatcaccg tcgtgactcg
     1501 tgccggcttg gccagaacgt cgagcaagtc gacgatctgc accatcacct cgcggtattc
     1561 accggtgatc ttttcctgct ccagcccggt caggcgctgc aggcggagct cgagtatcgc
     1621 ctgcgcctgc gcttccgaca gccgatagcc ctgcggagag ccgtgccgct gccagccgta
     1681 ctccggggcc aggccctcgg gcctggcggc gtcggccgcc gaacggttca gcatctcctc
     1741 gaccagcgcc gagcgccagg tcctggccat caacgccgcc ttggcctcgg ccggcgtcgc
     1801 cgccgccttg atcagcgcga tgatctcgtc tacgttcgat aacgcaacgg ccagcccttc
     1861 gagaatatgg ccgcggtcgc gcgccttgcg gagctcgaag cgcgtgcgcc gcacgacgat
     1921 ctctcgccgg tgctgcagga agaactcgag gaattgcttg aggttgagca ggcgcggctg
     1981 gccgtcgacc agcgcgacca tgttcatgcc gaagctgtcc tgcagctgcg tcagcttgta
     2041 cagattgttg agaatgattt cggtgacttc gccgcgcttg agctcgatga ccacgcgcat
     2101 gcccgatttg tcggactcat cacgcagatc ggagattccg tcgagctttt tctcgcgcac
     2161 cagctcgccg atccgcgtca gcagcgtcgc cttgttgacc tgatacggta gctcgtcgac
     2221 gatgatcgcc tgccgtccgc ccttctccag gtcctcgatg tgcgtgcggg cgcgtatgac
     2281 gacgcggcca cggccagttc gatagccttc ccggacacct tcagtgccga aaatgatgcc
     2341 cgcggtgggg aagtccggcg cgggaacgtg ttcgatcaac tcatcgactg tcgtgtccgg
     2401 attgtcgagc agcgtcaggc acgcggtgat gatctcggtg agattgtgcg gcgggatatt
     2461 ggtcgccatc ccgacggcga tgccggatga gccgttcaca agcagattcg gaatgcgcgt
     2521 cggcaatacc gtcggctcga gctccttgcc gtcgtagttg ggcgcgaaat cgacagtttc
     2581 cttgtcgatg tcggccagca tctcggcggt gatcttctcc agccggcatt cggtatagcg
     2641 gtatgccgcg ggtgcgtcgc cgtcgaccga gccgaagttg ccctggccgt cgatcagcgg
     2701 atagcgaagc gaaaaatcct gcaccatccg gaccagcgcc tcataggtcg cgctgtcgcc
     2761 gtgcggatga tatttgccga gcacgtcacc gacgacccgt gcgcatttga cgtagggccg
     2821 gttccagacg gtgttggatt cgtgcatcgc gaacagcacg cggcggtgca cgggtttcag
     2881 gccgtcgcgc acatccggca gtgcccgtcc gacgattacg ctcattgcgt aatcgaggta
     2941 ggaatgccgc atttctgctt cgaggcttac cggcagggtt tctttagcga actgctccat
     3001 cgtggaccgg ctctttatct agtgaaatgg ttacaatcgg cggatcgcaa atgaccgccg
     3061 cgcgagcctg aaatcgcccc gcgcagcccc gcgcggagcg gtgcgggcgt tcatagcgcg
     3121 aaacaatgaa ttttagcatg accgttgtat tgctgcgaca cttgccgata ttgcctttac
     3181 cgaatggccc atttcgcttt gcagcgtcaa tttccctatg ctatattggc cgggaagtcg
     3241 gcatttccag acgaacgcga aaaaaagttg ccgttacgct ccaaaatgaa cgggtagaac
     3301 tcaacaacta caggcagaga ggtcaaagtg atgaaaaatc gtctgatcgc aaccctcgtg
     3361 accggcgcgc tcgccgccac gatggccagt gcggccttcg gtcaagcgga tccgaaaaac
     3421 tccgcttatt ggctcga
//
LOCUS       DATANS010000515         1843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_781864, whole genome shotgun sequence.
ACCESSION   DATANS010000515 DATANS010000000
VERSION     DATANS010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781864"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VHT22_07580"
     CDS             <1..334
                     /locus_tag="VHT22_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX3137453.1"
                     /translation="LKHALKAALQDILPPDILERKKRGFGTPMGAWLKGDLAPLMREL
                     LSPASIEVRGLFRPPEVAALIAAHRANRLDGTDRLLTLINLEIWARMYLDGREPTDVA
                     DELKAVTS"
     gene            331..1563
                     /locus_tag="VHT22_07585"
     CDS             331..1563
                     /locus_tag="VHT22_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HEX3137454.1"
                     /translation="MKILYVCHRFPFPPKRGGKIRPFNMIRHLSARHEVHVASIARSM
                     EEAREGEGLAAHCASYDIGRVNNPVQTMRMLVRLPTPNPSSIGYFYSPQLAQRIDALL
                     AGTRFDLIFVHCSSVAPYVENVRGTPKILDFGDMDSQKWLEYARFKPFPLSLGYRLEG
                     AKMERAEKRLARHFDLCTATTRAEWETLESYRTGVASDWFPNGVDGEYFAPSGEAYDA
                     DTIVFVGRMDYYPNQECMFDFCARTLPLLHARRPSLKLMIVGADPSPRVRRLGELPGV
                     SVTGSVADVRPYLQRSALMIAPLNIARGTQNKILEAMASGVPVVTSRVAAGGVDAADP
                     EHFLAASSPDEYAAAILRIVDDPVERQRLSRAGRARMLSHHSWDKSMQRLDAIIDRCL
                     ASQGRTSRDTRATAGALA"
     gene            1560..>1843
                     /locus_tag="VHT22_07590"
     CDS             1560..>1843
                     /locus_tag="VHT22_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose dehydrogenase"
                     /protein_id="HEX3137455.1"
                     /translation="MNISIFGLGYVGAVSLACLARDGHRVVGVDVDQAKLDLIAAGRT
                     PVVEEGMVQLMADTVASGRVSVSRDTRRAVLETDVSLICVGTPSAPNGSQ"
BASE COUNT          318 a          601 c          598 g          326 t
ORIGIN      
        1 gctcaagcac gcgctcaagg ctgcgctgca ggacatcctc ccgccagaca tcctcgagcg
       61 caagaaacgc ggcttcggca cgccgatggg ggcttggctg aagggcgatc tcgcgccgct
      121 gatgcgcgag ctgctgtcgc cggcatcgat cgaggtgcgc ggattgttcc ggccgccaga
      181 agtcgccgcg ctgatcgcgg cgcatcgcgc caaccgcctg gacgggacgg accggctatt
      241 gactctgatc aacctcgaaa tctgggcgcg catgtatctc gacggccgcg agccgaccga
      301 tgtcgccgac gagctcaagg cggtgacgtc gtgaagattc tctacgtctg ccatcgcttc
      361 ccgtttccgc cgaagcgagg cggcaagatc cgtccgttca acatgatccg ccacttgtcg
      421 gcccggcacg aagtccacgt cgcgtcgatc gcacgctcga tggaggaagc gcgggaaggt
      481 gagggcttgg cagcgcactg cgcaagctac gacatcggaa gagtcaacaa tccggtacag
      541 acgatgcgga tgctggttcg ccttccaacg ccgaatccgt cgtcgattgg atatttttat
      601 tcgccgcagc tggcgcagcg catcgatgca ctgctcgccg gcacacgctt cgacctgatc
      661 ttcgtgcatt gctcgtcggt cgcgccgtat gtcgagaatg tccgcggcac gcccaagatc
      721 ctcgacttcg gcgacatgga ctcgcaaaag tggctcgagt acgcgcgctt caagcctttc
      781 cccttgtcgc tgggctaccg gctggaaggc gccaagatgg agcgcgccga aaagcggctt
      841 gcccggcatt tcgacctgtg cacggcgacg acgcgagccg aatgggaaac cctcgaaagc
      901 tatcgcaccg gtgttgcctc ggactggttc ccgaacggcg tcgacggcga atatttcgca
      961 ccgagcggcg aagcctacga tgctgatacg atcgtcttcg tcggccgaat ggactactac
     1021 ccgaatcagg agtgcatgtt cgatttctgc gccaggacac tgcctttgct gcacgcacgc
     1081 cgcccatccc tcaagctgat gatcgtcggc gccgatcctt cgccgcgcgt gcgacggctt
     1141 ggagagctcc ccggtgtcag cgtgaccgga tcggtggccg acgttcggcc gtacctgcag
     1201 cgctcggcgc tgatgatcgc accgctaaat atcgcgcggg ggacacagaa caagatcctc
     1261 gaagcgatgg cgagcggcgt tccggtcgtg accagccgcg tcgctgccgg cggcgtcgac
     1321 gccgccgatc ccgaacattt cctcgcggcg tcatcgcctg acgagtacgc ggcggcgatc
     1381 ctgcgcatcg tcgacgatcc ggtcgagcgt cagcgcctgt cgcgcgctgg gcgtgctcgc
     1441 atgctgtcgc atcactcctg ggacaagtcg atgcagcgcc tcgacgccat catcgatcgc
     1501 tgtctggcaa gccagggccg gacatcgcgg gacacgcgcg cgaccgctgg agcactggca
     1561 tgaacatcag catcttcggc ctgggctacg tgggcgcggt atcgctcgcc tgtctggcgc
     1621 gcgatggcca tcgcgtcgtc ggcgtcgacg tcgaccaggc gaagctcgac ctgatcgccg
     1681 ccggccggac accggtggtc gaggagggca tggtgcagct gatggccgat acggttgcca
     1741 gtggacgtgt ttcggtcagc agggacactc gccgggcggt gctggagacc gacgtctcgc
     1801 tgatctgcgt cggcacgcca tcggcgccga acggcagcca gga
//
LOCUS       DATANS010000516         2901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_279457, whole genome shotgun sequence.
ACCESSION   DATANS010000516 DATANS010000000
VERSION     DATANS010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279457"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VHT22_07595"
     CDS             complement(<1..300)
                     /locus_tag="VHT22_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3137456.1"
                     /translation="MSTVSPARTRLPSGRDTDAAYLAAIGERVREIRARRGMSRRTLA
                     HDSGVSERYLASLESGRGNVSILLLKQIAQAIDVPVEQLTRNGPEASTEVALLMQW"
     gene            433..951
                     /locus_tag="VHT22_07600"
     CDS             433..951
                     /locus_tag="VHT22_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEX3137457.1"
                     /translation="MLNVNILVGTMTGTAQLVAQEIELAFADAQTGIDVTFMDALDRR
                     VFERPGVFLICTSTYGQGDVPDNAKTFYADLAACRESLAHVHYGVFALGDSTHVGTYC
                     FGGRRFDEALAARGARRIGEVMQHNASGGTLPEDVALEWFPEWLRQARTTLEAAESDP
                     AVAAQDARPASD"
     gene            1007..2605
                     /locus_tag="VHT22_07605"
     CDS             1007..2605
                     /locus_tag="VHT22_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEX3137458.1"
                     /translation="MTARTPGFAQADHSASPPRLSIPRAYNAAVDLVERNLAAGRSGH
                     TAFIDDAGRYTYGELAQRVNRCANAWKRLGLRTEDRVVLLLHDSIDFPTAFLGAIRAG
                     VVPIPCNTLLTAADYEYLLADSRARAIVVSAPLASVIAPLAARVDALEHVIVSGGAVD
                     GTVAFADWIASESADAPVAATTADDVCFWLYSSGSTGSPKGAVHLHADLIQTAELYAR
                     PVLGIAPDDVVFSAAKLFFAYGLGNALTFPLSVGATSVLMAERPTPAAVFRRLVGERP
                     TIFYGVPTLYAAMLASAEFPGRDRLGLRRCVSAGEALPADLGKRWRDRTGVDILDGLG
                     STEMLHIFLSNRPDDVCYGTTGRPVPGYALRVVDEQGDPVATGEIGELQVNGPTSSPY
                     YWNNRAKSLATFLGPWTRSGDKYIVDADGRYVYCGRTDDMLKVGGVYVSPFEVEAALA
                     THEAVLESAVVGCADASELIKPKAFVVLKPGHTPGPALAAALQEHVKSLLAPFKYPRW
                     IEFVADLPKTATGKIQRFKLRAPA"
     gene            2685..>2901
                     /locus_tag="VHT22_07610"
     CDS             2685..>2901
                     /locus_tag="VHT22_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX3137459.1"
                     /translation="MTDRRQFIQQAGLLASAAAIGGLTPTIARAQNAKIRVGMMLPYT
                     GTFAALGTAITQGFNLAVEENGGMLGGR"
BASE COUNT          487 a         1006 c          934 g          474 t
ORIGIN      
        1 ccattgcatc agcagcgcaa cctcggtcga cgcctcgggg ccatttcgcg tcaactgctc
       61 gaccggcaca tcgatcgcct gcgcgatctg cttcaacagc aggatcgaaa cattgccccg
      121 ccccgattcc agcgatgcga gatagcgctc ggacacgccc gaatcatgcg ccagcgtgcg
      181 gcgcgacatc ccgcgccgtg cgcggatctc gcgaacgcgc tcaccgattg cggccagata
      241 ggcggcatca gtgtcgcggc cggaaggtag ccgcgtacgc gccggcgaga ccgtgctcat
      301 gtccggcgcg agcccccggg ttcgcgccgg gctcggctgg tgcgataatg cattttattg
      361 catccagatt tctggatatg tactatagtt catgaccgtc ccgatgacaa gcccgatgac
      421 tagcctcgac cgatgctgaa cgtcaatatc ctggtcggca cgatgaccgg taccgcgcag
      481 ctggtcgcgc aggagatcga gctcgccttc gccgacgcgc aaacaggaat cgacgtcacg
      541 ttcatggatg cgctcgatcg acgcgtgttc gagcggcccg gcgtattcct catctgcacg
      601 tcgacctacg gccagggcga cgtgcccgac aacgccaaga ccttctacgc cgatctcgcc
      661 gcgtgccgcg agtcgctggc gcacgtgcat tacggcgtgt ttgcgctcgg cgacagcacg
      721 cacgtcggca cctactgttt cggcggcagg cgtttcgacg aagcgctggc ggcgcggggg
      781 gcgcggcgca tcggcgaagt gatgcagcac aacgccagcg gcggcacgct gccggaagac
      841 gtggcgctgg aatggtttcc ggaatggctg cggcaggcgc gcaccaccct cgaggcagcg
      901 gaatccgacc ctgcggtcgc ggcgcaagac gcgcggcccg caagcgattg acgaggcccg
      961 gcatgcgccg ccacaatcgc cagtcccgct tccagccgac gcgactatga ccgcacggac
     1021 acctggcttt gcccaggcag accattcggc gtcgccgccg cgcttgagca ttccccgcgc
     1081 ctacaacgcc gccgtggacc tggtcgagcg caatctcgcc gccggtcgca gtgggcacac
     1141 tgcgttcatc gacgatgccg gacgctatac ctacggcgag ctcgcgcagc gcgtcaaccg
     1201 ctgcgccaac gcctggaagc gtctgggcct tcgcaccgag gatcgagtcg tcctgctgct
     1261 gcatgacagc atcgattttc cgacggcgtt tctgggcgcg atccgcgccg gcgtcgtgcc
     1321 gatcccctgc aatacgctgc tcaccgccgc cgactacgaa tatctgctgg ccgacagccg
     1381 cgcgcgggcc atcgtcgtct ccgcgccgct cgcgtcggtg atcgcgccac tcgccgcccg
     1441 cgtcgatgcg ctcgagcacg tcatcgtctc cggcggcgcc gtcgacggca ccgtggcctt
     1501 tgccgactgg atcgcgtccg agtcggccga tgcgccggtc gcggcgacga cggcggacga
     1561 cgtctgcttc tggctgtatt cgtcgggatc gaccggcagc ccgaagggcg ccgtgcatct
     1621 gcatgccgac ctgatccaaa ccgcggagct ctatgcgcgc ccggtactcg gcatcgcacc
     1681 ggacgacgtg gtgttctccg cggccaagct ctttttcgcc tacgggctgg gcaatgccct
     1741 gacttttccg ctttcggtcg gcgcgaccag cgtgctgatg gccgagcggc cgacgccggc
     1801 ggcggtgttc aggcgcctgg tcggcgagcg gccgacgatt ttctacggcg tgccgaccct
     1861 gtacgcggcg atgctcgcca gcgcggaatt tccggggcgc gaccggctcg gtctgcgccg
     1921 ctgcgtctcc gccggagagg cgctgccggc ggacctcggt aagcgctggc gcgacaggac
     1981 cggcgtcgac atcctcgacg gcctgggctc gaccgagatg ctgcatatct tcctgtccaa
     2041 tcgtcccgat gacgtctgct acggcacgac cggcaggccg gtgcctggct acgccctgcg
     2101 cgtcgtcgac gaacaaggcg atccggtcgc gaccggcgag atcggcgagc ttcaggtcaa
     2161 cggaccgacg agctcgccgt actactggaa caatcgcgcc aagagcctgg ccacgtttct
     2221 cgggccgtgg acccgcagcg gcgacaagta catcgtcgac gccgacggcc gctacgtcta
     2281 ttgcggccgc accgacgaca tgctcaaggt cggcggcgtg tatgtctcgc cattcgaggt
     2341 cgaggcggcg ctggcaacgc acgaggccgt tctcgaatcc gcggtcgtcg gctgcgccga
     2401 tgcgagcgag ctgatcaagc cgaaggcctt tgtcgtgctc aagcccggac atacgcccgg
     2461 gcccgccctc gctgcggcgc tgcaggagca cgtcaagtcg ctgcttgcgc cgttcaaata
     2521 tccgcgctgg atcgaattcg tcgccgactt gccgaagacc gcgaccggca agatccagcg
     2581 cttcaagctg cgcgcaccgg cgtaggacac cagaacatga cggaatgccc acccgctgct
     2641 accatcgcag cgccgacaag atcaaaacct cggaggagaa gcaaatgaca gaccggcgtc
     2701 agttcatcca gcaggcagga cttcttgcat cggcagccgc aatcggcggg cttacgccaa
     2761 cgatcgcgcg agcacagaac gccaagatca gagtcggcat gatgctaccg tacacgggaa
     2821 ccttcgcggc gctcggcacg gcgatcactc agggcttcaa tctcgcggtc gaggaaaacg
     2881 gcggcatgct cggcgggcgc g
//
LOCUS       DATANS010000517         1931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_234526, whole genome shotgun sequence.
ACCESSION   DATANS010000517 DATANS010000000
VERSION     DATANS010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234526"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VHT22_07615"
     CDS             <1..620
                     /locus_tag="VHT22_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX3137460.1"
                     /translation="EKAPEAFRTISEVASDLDLPQHVLRFWETRFQQIKPLKRGGGRR
                     YYRPDDIDLLRGIRYLLYSEGYTIRGAQRILKDHGVKFVQNVWRAGAPQPTRAMEAAE
                     PEIPEAPPLRPQPREARRGDADSAHRRLFGLLPSFLSSDPDEERLAESRRADADTMIL
                     PSPLVPRVSEAASLPAEGMRKLQETLRELQECRRLLDAALAEASA"
     gene            789..>1931
                     /locus_tag="VHT22_07620"
     CDS             789..>1931
                     /locus_tag="VHT22_07620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEX3137461.1"
                     /translation="MATSKNPEPRATPTPAKPESDDRRVEPPQQPAADEERYARALES
                     ISHGVYDWDIARGTVYYSPLMRSIFGMAADATLTPAESGSRIHRDDLEQYRRAIVEHL
                     KGVTPRLLVEYRYLANDNTWRWARQSGIAQRGPDGRATRLVGATSDITDLKQRERDLA
                     AARAEIEATREDMRTVLDNMSDGITLIDENFNWKFSNKQFSTFLDVPPEITKPGISCY
                     DVIRYQALRGDFGEQSDIEQVVQERAALMRTPGGCRYERLTKSGRYIEFTHRPLADGT
                     LLGVYRDITELKRRESEQVDARDAAEMALAEAERERAEAEAANQAKSTFLATMSHEIR
                     TPMNGVLGMMEVLDRQGLSASQQRTVATMRDSAQALLRIIDDVLDFS"
BASE COUNT          379 a          651 c          619 g          282 t
ORIGIN      
        1 cggaaaaggc acccgaggcg tttcgtacga tcagcgaagt ggcgagcgat ctcgacttgc
       61 cgcaacatgt gttgcgcttc tgggagactc gcttccagca gatcaagccc ttaaagcgcg
      121 gtggcggccg ccgctactat cggcccgacg atatcgatct gctgcgcggc atccgctacc
      181 tcttgtacag cgagggttac acgatccgcg gcgcgcagcg gatcctgaag gatcacggcg
      241 tcaagttcgt ccagaacgtc tggcgggccg gcgcgcccca gcctacgcgc gcaatggaag
      301 ctgccgagcc cgagattccc gaggcaccgc cgctgcggcc gcagccgcgc gaggcgcggc
      361 gcggcgatgc ggattccgcg catcggcggc tgttcggctt gctgccttca ttcctcagca
      421 gcgatcccga cgaggaacgt ctggcagaga gccgccgggc cgatgcggac accatgattc
      481 ttccctcgcc tttggtgccg cgggtgagcg aagcagcctc tctgccggcg gagggcatgc
      541 gcaagctgca ggaaacattg cgggaactgc aggaatgccg gcggctgctt gacgcagcgc
      601 tcgcggaagc ttcggcctga ccgggccggt tcccgccatc atgacggcaa ccgttcccgc
      661 cacatggcgg caccaaccgg cgcctccccg cgttgacccc ggaccctcag acgatacaca
      721 cgatacaaat tggtaagccc gcgatacgcg aacgatacag ctaggcccca caatgccgtg
      781 gggaacgaat ggcaacgtca aaaaatcccg agccacgcgc tacgccgaca ccggccaagc
      841 cggaatctga tgatcgccgc gtcgaaccgc cgcagcagcc tgcagcggac gaggagcgtt
      901 acgcgcgcgc gctggagtcg atcagtcacg gcgtttacga ctgggatatc gccaggggca
      961 cggtctacta ctcgccgctg atgcgctcga ttttcggcat ggcggccgac gcgacgctga
     1021 cgccggccga gtccggcagc cgcatccatc gtgacgacct cgagcagtat cgcagggcga
     1081 tcgtcgaaca cctcaagggc gtcaccccgc gcctgctcgt cgagtatcgc tacctcgcca
     1141 acgacaatac ctggcgatgg gcccgccaga gcggcatcgc gcaacgcggt ccggacggac
     1201 gggcgacgcg cctggtcggc gccaccagcg acatcacgga tctcaagcag cgcgagcgcg
     1261 atctcgcggc tgcccgcgcc gagatcgagg cgacgcgcga ggacatgcgc acggtgctcg
     1321 acaatatgag cgacggcatc acgctgatcg acgagaactt caattggaaa ttcagcaata
     1381 agcagttcag cacttttctg gacgtgccgc cggagatcac caagcccggc atctcctgtt
     1441 acgacgtgat ccgctaccaa gcgctgcgcg gcgactttgg cgagcagagc gacatcgaac
     1501 aagtcgtgca ggaacgcgcc gcgctgatgc gcacgcccgg cggctgccgc tacgagcggc
     1561 tgaccaagag cggacgctac atcgagttca cgcaccggcc gctcgccgac ggcacgctgc
     1621 tcggggtgta ccgcgacatc accgagctga agcggcgcga gagcgagcag gtcgacgcga
     1681 gagacgctgc cgaaatggcg ctcgccgaag ctgaacgtga gcgcgccgag gcggaagccg
     1741 ccaaccaggc gaaatcgacc ttccttgcca cgatgagtca cgagatccgc acgcccatga
     1801 atggcgtgct gggcatgatg gaggtgctcg accgccaggg cttgagcgcc agccagcagc
     1861 gcaccgtcgc aaccatgcgc gattcagcgc aggcgctgct gcggatcatc gacgacgtcc
     1921 tcgacttctc c
//
LOCUS       DATANS010000518         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_109585, whole genome shotgun sequence.
ACCESSION   DATANS010000518 DATANS010000000
VERSION     DATANS010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109585"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VHT22_07625"
     CDS             <1..820
                     /locus_tag="VHT22_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX3137462.1"
                     /translation="YGISTPVTVRLKWAGTKSAPVDDKPVLYVLSIGVSEYIDPENRL
                     KFAAKDAVDFAAIVQSQKGKLYRDVVVKLLTDAKASRADVLAGFEWLQKQVTPKDVGI
                     VLIAGHGINDDRGNYYYLPVNADIDKLETTGVPFSAIKAHLSSLQGKGLLFVDTCHSG
                     NVMGRKRGFSTDTTAILNELSSPEYGLVVIASSTGKQYSFENTDWGNGAFTKALVEGL
                     SGRADLKKRGRITHKMLDFYVSDRVEELTQGRQTPVNTSPLGVPDYTIAVIGPG"
     gene            complement(833..>1574)
                     /locus_tag="VHT22_07630"
     CDS             complement(833..>1574)
                     /locus_tag="VHT22_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3137463.1"
                     /translation="RTTYPNAHWISPHWHARAQFIFAVAGTMRVRTARHVWIVPPTRA
                     LWVPARTVHEVQMYGEVKMRSLYVDDAAGAGMPASCVVLNVTPLLKELVVRAVALPES
                     YDEHGDDGLVMQLLMAEVRRLPRCAFDLPLPESPDLMRLCERLLDDLSARRPCNLDAR
                     ELKTSTRTLYRRFLRETGITFARWKQQARLLESIRRLAEGVAVTTVALDLGYASPSAF
                     SSMFRRAVGAPPRVFTAGEISRDTPRAN"
BASE COUNT          314 a          512 c          495 g          253 t
ORIGIN      
        1 atacggcatc agcacgccgg tcaccgtgcg gcttaagtgg gcgggcacga aatccgcgcc
       61 ggtcgacgac aagccggtgc tgtacgtgct gtctatcggc gtgtcggaat atatcgaccc
      121 ggaaaaccgg ctcaaattcg ccgccaagga cgcggtcgac ttcgcggcca tcgtgcaaag
      181 ccagaagggc aagctctatc gcgacgtcgt cgtcaagttg ctgaccgacg cgaaagcgag
      241 ccgtgcggac gtgctcgccg gcttcgaatg gctgcaaaaa caggtcacgc ccaaagacgt
      301 cggcatcgtg ctgatcgccg gccacggcat caacgacgat cgcggcaatt attactatct
      361 gccggtcaac gccgatatcg acaagctcga gacgaccggc gtgccgttca gcgcgatcaa
      421 ggcgcatctg tcgagcctgc aaggcaaagg cctgctgttc gtggatacct gccactcggg
      481 caacgtaatg ggcaggaagc gcgggttttc aaccgacacg accgcgatcc tcaacgagct
      541 gtcgagtccc gagtacgggc tcgtcgtgat cgcatcatcg accggcaagc aatactcgtt
      601 cgagaatacc gattggggca atggcgcgtt taccaaggcg ctggtcgaag gcttgagcgg
      661 acgcgccgat ctcaagaagc gcggccgcat cacgcataag atgctcgact tctacgtgtc
      721 ggaccgcgtc gaagagctca cgcaaggccg ccagacgccg gtcaacacga gcccgctcgg
      781 cgtgcccgac tacacgatcg cggtaatcgg ccccggctaa actaactggc gttcagttcg
      841 cgcggggcgt atcccgcgaa atttcacccg cggtgaatac gcgcggcggc gcgcccaccg
      901 cgcgccggaa catggacgaa aacgcgctgg ggctcgcata gccgagatcg agcgcgacgg
      961 tcgtgaccgc cacgccttcg gccagccggc ggattgattc gagcaaacgg gcctgctgct
     1021 tccagcgcgc aaacgtaatg cccgtctcgc gcaggaagcg ccggtaaagc gtgcgcgtgc
     1081 tggtcttgag ttcgcgcgcg tcgagattgc acggacgccg cgcggataga tcgtcgagca
     1141 gccgctcgca taagcgcatg agatccggac tctccggcag aggcaggtca aacgcgcagc
     1201 gcggcagccg ccgcacttcg gccatcagaa gttgcataac gagcccgtcg tcgccgtgct
     1261 cgtcgtagct ttcgggcaag gccaccgcgc gcaccacgag ctctttcagc agcggcgtca
     1321 cgttcaacac cacgcacgat gccggcatgc ccgcgccggc ggcatcgtcc acgtacagac
     1381 tgcgcatttt cacttcaccg tacatctgca cttcgtgcac ggtgcgcgcc ggcacccaca
     1441 gggcgcgcgt cggcggcacg atccatacat gccgcgcggt gcgcacgcgc atcgtgccgg
     1501 ccaccgcgaa tatgaattgg gcgcgcgcgt gccagtgcgg tgaaatccaa tgcgcgttcg
     1561 ggtacgtcgt gcgg
//
LOCUS       DATANS010000519         1808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_699590, whole genome shotgun sequence.
ACCESSION   DATANS010000519 DATANS010000000
VERSION     DATANS010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699590"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VHT22_07635"
     CDS             <1..241
                     /locus_tag="VHT22_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX3137464.1"
                     /translation="IFVAAGFEWREPGCSMCLGMNEDQLSPGERCASTSNRNFEGRQG
                     AGGRTHLVSPAMAAAAAVAGHFVDVRTLAQEKNHG"
     gene            234..872
                     /gene="leuD"
                     /locus_tag="VHT22_07640"
     CDS             234..872
                     /gene="leuD"
                     /locus_tag="VHT22_07640"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX3137465.1"
                     /translation="MDKFISREGLVAPLDRANVDTDAVIPKQFLKSIKKTGYGPNLFD
                     EWRYLDHGEPGMDNAKRPLNPDFVLNQPRYQGAQILLARENFGCGSSREHAPWALLQY
                     GFQAVIAPSFADIFFNNSFKNGLLLIKLDAKTVDRLFKEAAAQQGYRLAIDLADQSVT
                     TPGGEAIKFEIDPFRKHCLMNGLDEIGLTLEHADKIKAFEAKRREQQPWLFN"
     gene            935..>1808
                     /gene="leuB"
                     /locus_tag="VHT22_07645"
     CDS             935..>1808
                     /gene="leuB"
                     /locus_tag="VHT22_07645"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX3137466.1"
                     /translation="MKIAVLAGDGIGPEIIAQAVKALDVLRRDGLKLELEEAPYGGAG
                     YDAQGDPLPESTLKLARAADAVLCGAVGGPQYDALPRDKRPEQGILRIRKELQLFANL
                     RPAMLFDELVSASSLKPELVKGLDILILRELTGDIYFGEPRGRRTNASGEREGYDTMH
                     YSEPEIRRIADVGFKAAMKRGKKLCSVDKENVLETSRFWREVVKDVAKEYPQVELSHM
                     YVDNAGMQLVRNPKQFDVIVTGNMFGDILSDEASMLTGSIGMLPSASLDANNKGLYEP
                     SHGSAPDIAGKNIAN"
BASE COUNT          362 a          552 c          585 g          309 t
ORIGIN      
        1 gattttcgtt gccgcgggtt tcgaatggcg cgagcccggg tgctcgatgt gcctcggcat
       61 gaacgaggac cagttgtcgc ccggcgagcg ctgcgcgtcg acgtccaatc gcaatttcga
      121 ggggcgccag ggtgcgggcg ggcgcacgca tctcgtgagc ccggcgatgg ccgccgcggc
      181 tgcggttgcc ggtcattttg tcgatgtgcg cacgctggcg caggaaaaaa atcatggata
      241 aattcatctc acgcgaaggc ctggtggcgc cgctcgaccg cgccaatgtc gacactgatg
      301 cggtgattcc gaagcagttc ctgaaatcga tcaagaagac cggttacggc cccaatctct
      361 tcgacgagtg gcgctacctc gatcacggcg agccgggcat ggacaacgca aagcggccgc
      421 tcaatcccga cttcgtgctg aaccagccgc gttaccaggg cgcgcagatt ctgctcgcgc
      481 gcgaaaattt cggctgcggc tcgtcgcgcg agcacgcacc gtgggcgctg ctgcagtatg
      541 ggtttcaggc ggtgatcgcc ccgagcttcg cggacatatt tttcaacaac agcttcaaga
      601 acgggctgct gttgatcaag ctcgacgcca agaccgtcga ccgcttgttc aaggaggccg
      661 cggcgcagca gggttaccgt ctcgcaatcg atctggcgga ccagagcgtc accacgccgg
      721 gcggcgaagc gatcaagttc gagatcgatc cgttccgtaa gcattgcctg atgaatggcc
      781 tcgatgaaat cgggctcacg ctcgagcacg cagacaagat caaggccttc gaagcgaagc
      841 gacgcgagca gcagccctgg ttgttcaatt gagcccgcat atggcggcgc acgctgatgc
      901 gtaccgtcaa gtgagcggct attaagggta gtcgatgaag atcgcagtgt tggccggcga
      961 tgggatcggc cctgaaatca tcgcgcaggc ggtaaaagcg ctcgacgtgc tacggcgcga
     1021 cggcctcaag ctcgagctgg aagaagcgcc gtacggcggc gccgggtacg acgcgcaagg
     1081 cgatccgctg ccggaatcca cgctcaagct tgcgcgcgcc gcggacgcgg tgctgtgcgg
     1141 cgcggtcggc ggcccgcaat acgacgcgtt gccgcgcgac aagcgccccg agcagggcat
     1201 cctgcgcatc cgcaaagaat tgcagctctt cgccaacctg cgtcccgcga tgctgttcga
     1261 cgagctggtc agcgcatcct cgctaaagcc ggagctggtc aaaggcctcg atatcctgat
     1321 cctgcgcgag ctgaccggcg atatttactt cggcgagccg cgcggccggc gcacgaacgc
     1381 gagcggcgag cgcgaaggct acgacacgat gcattattcg gagcccgaga tccggcgcat
     1441 cgcggacgtc ggcttcaagg cggcgatgaa gcgcggcaaa aagctctgct cggtcgacaa
     1501 ggaaaacgtg ctcgagacga gccggttctg gcgcgaagtc gtcaaagacg tcgccaaaga
     1561 gtatccacag gtcgagctgt cgcatatgta cgtcgacaac gcgggaatgc agctcgtgcg
     1621 caatccgaag cagttcgacg tgatcgtcac gggcaacatg ttcggcgaca tcctctcgga
     1681 cgaggcctcg atgctgaccg gctcgatcgg catgctgccg tcggcgtcgc tcgatgcgaa
     1741 caacaagggt ctgtatgagc cttcgcacgg ctccgccccc gacatcgccg gcaagaatat
     1801 cgccaatc
//
LOCUS       DATANS010000520         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_489145, whole genome shotgun sequence.
ACCESSION   DATANS010000520 DATANS010000000
VERSION     DATANS010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489145"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /gene="phnE"
                     /locus_tag="VHT22_07650"
     CDS             complement(<1..776)
                     /gene="phnE"
                     /locus_tag="VHT22_07650"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009360654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEX3137467.1"
                     /translation="MNGPAVDVRAAAIAAGRRRGRALRSLTYLAIFGAVLWSIQVIMI
                     ADTDWARLAASSAGKTLASFLEFDYRLLPELVDPTIDTALMATLATLAGLLLAIPVAW
                     LGAANISPLGRASYVVGRFLMTVSRSVHEIVWGLIFVSAVGLGALAGILAMGVRSVGF
                     ISKIIAEAIEDVQPGPIEAMRAVGGNRFQVLWYGILPQIVPVVLGTMIFEWDINIRRS
                     TIMGLVGAGGLGLVLFRQMAMLNYGGVATVILAVLCLIILG"
     gene            complement(773..>1504)
                     /gene="phnC"
                     /locus_tag="VHT22_07655"
     CDS             complement(773..>1504)
                     /gene="phnC"
                     /locus_tag="VHT22_07655"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEX3137468.1"
                     /translation="VRNLRAGYAGREILKGVSFQVDADDFFAIIGPSGAGKSTLIRCV
                     NRLVEPTGGQVLFHGVDLLQLDQVRLREARRNIGMIFQEFNLIERLSVMDNVLTGRLG
                     YTGTLRSLMRMFTAEDIRQALALLDRVGLGEYVDKRADRLSGGQRQRVGIARALIQNP
                     RMLLVDEPTSSLDPKISREVMGLIREIAREFHIPVLCNIHDVELATEFCTHVIGLQDG
                     VKKFEGRPAELNAQALHEIYAMEVL"
BASE COUNT          291 a          501 c          476 g          236 t
ORIGIN      
        1 ccgagaatga tcaagcacag cacggcgagg atcaccgtcg cgacgccgcc gtagttgagc
       61 atcgccatct ggcgaaagag cacgagcccg agaccgccgg cgcctaccag ccccatgatc
      121 gtcgagcggc ggatgttgat gtcccactcg aagatcatcg tgccgagcac caccggcacg
      181 atctgcggca agatgccata ccacagtacc tggaaccggt tgccgccgac ggcgcgcatc
      241 gcctcgatcg gcccgggttg aacgtcttcg atcgcctcgg cgatgatctt ggagatgaag
      301 ccgaccgacc gcacgcccat cgcgaggatg ccggcgagcg ctcccagccc gaccgcggaa
      361 acgaaaatca gcccccacac gatttcatgc accgaacgcg atacggtcat caggaaccgg
      421 ccgacgacat acgaagcgcg gccgagcgga ctgatgttcg ccgcgccgag ccaggcgacg
      481 ggaatcgcga gcaacagccc ggccaatgtc gcgagcgtcg ccatcaacgc ggtgtcgatc
      541 gtcgggtcga cgagctccgg cagcaagcgg tagtcgaact cgaggaaact cgcgagcgtc
      601 ttgccggcgg agcttgcggc gagccgtgcc caatcggtgt cagcgatcat gatgacctgg
      661 atgctccaca gcaccgcgcc gaatatcgcg agataggtca gtgaacgcag cgcccggcca
      721 cgccggcggc ctgcggcgat cgcggcggcg cgcacgtcga ccgccggccc gttcacagga
      781 cctccatcgc gtagatctcg tgcagcgcct gcgcattcaa ttcggccgga cggccctcga
      841 atttcttgac gccgtcctgc aggccgatca cgtgcgtgca gaattcggtc gcgagctcga
      901 cgtcgtggat gttacacagg acggggatat ggaattcccg cgcgatctcg cggatcaatc
      961 ccatgacttc tcgcgagatc ttcggatcga ggctcgacgt cggctcgtcg accagcagca
     1021 tgcgcggatt ctggatcagc gctctggcga tgccgacgcg ctgccgttgc cccccggaga
     1081 ggcgatccgc acgcttgtcg acgtattcgc ccaggccgac gcgatcgagc agcgcaagcg
     1141 cctgccggat gtcctcggcg gtgaacatgc gcatcaggct gcgcagcgtc ccggtatagc
     1201 cgagcctgcc ggtaagaacg ttatccatca ccgacagccg ctcgatcagg ttgaattcct
     1261 ggaagatcat gccgatattg cgccgcgcct cgcgcaggcg cacctggtcg agctggagaa
     1321 ggtcgacgcc gtggaacagc acctggccgc cggtcggttc gaccaggcgg ttcacgcatc
     1381 ggatcaaagt cgatttcccc gcgccgctgg ggccgatgat cgcgaaaaaa tcgtcggcgt
     1441 caacctggaa gctgacgccc ttgaggattt cacgcccggc gtagccggcg cggaggttcc
     1501 tgac
//
LOCUS       DATANS010000521         1565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_782204, whole genome shotgun sequence.
ACCESSION   DATANS010000521 DATANS010000000
VERSION     DATANS010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782204"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /gene="rpoD"
                     /locus_tag="VHT22_07660"
     CDS             <1..868
                     /gene="rpoD"
                     /locus_tag="VHT22_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX3137469.1"
                     /translation="QFIKHEKRQIDTIRDDIRTLASETGLEITEFRRIVKAVQKGERE
                     ARQAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFST
                     YATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQILQETGQEPDPALLAEKM
                     EMPEEKIRKILKISKEPISMETPIGDDDDSHLGDFIEDASTVAPIDAAVNASLRDVCK
                     DVLDTLTPREAKVLRMRFGVEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSR
                     SEKLRSFIEGEG"
     gene            complement(926..>1565)
                     /locus_tag="VHT22_07665"
     CDS             complement(926..>1565)
                     /locus_tag="VHT22_07665"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3137470.1"
                     /translation="LDPVVVSPIAAVVARASVNVDPSRIRLADGYRHMAIHPYPVELE
                     TSLHLRSGEALKLRPIRPEDADLECAFVAGMSEQSRYRRFMQHLPTLTPQMLARFTQV
                     DYDRELALIALDGDPAAEAIVAVARYVGNPDHESAEFAIAVADAWHGRGLGRAMMQAL
                     IACATKRGFRRLVGVVLASNEPMLAMCRKLGFAVERDPEAPEQVVVTLELGR"
BASE COUNT          326 a          499 c          505 g          235 t
ORIGIN      
        1 ccagttcatc aagcacgaaa agcgccagat cgacacgatc cgcgacgaca tccgcacgct
       61 ggcctccgaa acgggtctcg agatcaccga gttccgccgt atcgtcaagg ccgtgcagaa
      121 gggcgagcgc gaggcgcgcc aggccaagaa ggagatggtc gaagccaatc tgcggctcgt
      181 gatctcgatc gccaagaaat acaccaaccg cggcctgcag ttcctcgacc tgatccagga
      241 aggcaacatc ggcctgatga aggcggtcga caagttcgag taccgccgcg gctacaagtt
      301 ctcgacctac gccacgtggt ggatccgcca ggcgatcacg cgctcgatcg ccgaccaggc
      361 gaggacgatc cgcattccgg tgcacatgat cgagacgatc aacaagatga accgcatctc
      421 gcggcagatc ctgcaggaga ccgggcagga gcccgatccg gcgctgctgg cggaaaagat
      481 ggaaatgccc gaggagaaga tccgcaaaat cctgaagatc tccaaggagc cgatctcgat
      541 ggaaacgcct atcggcgacg acgacgattc gcatctgggc gatttcatcg aggacgcgag
      601 cacggtggcg ccgatcgacg cggcggtcaa cgcaagcctg cgcgacgtgt gcaaggacgt
      661 gctggacacg ctgaccccgc gcgaagcgaa ggttttgcgc atgcgcttcg gcgtcgagat
      721 gaacaccgac catacgctgg aggaagtcgg caagcagttc gacgtcacgc gcgagcgcat
      781 ccgccagatc gaggccaagg cgctgcgcaa gctccgccac ccgtcgcgct cggaaaagct
      841 gcgcagcttc atcgagggcg agggctaggc agtttcggca agtggatgta acggaaaaac
      901 gccgcctcgc gcggcgtttt ttcttctacc tgcccagctc cagggtcacc accacctgct
      961 cgggagcttc gggatcgcgc tcgacggcga agccgagctt ccggcacatc gcgagcatcg
     1021 gctcgttgct cgcgagcacg acaccgacga gacgacgaaa gccacgcttg gtcgcgcatg
     1081 cgatcagcgc ctgcatcatc gcgcggccga gcccgcgccc atgccaggcg tcagcgaccg
     1141 cgattgcgaa ctccgcgctc tcgtggtcgg gattgccgac atagcgcgcg acggcgacga
     1201 tcgcttccgc ggcggggtcc ccgtctagcg caatcagcgc gagctcgcgg tcgtaatcga
     1261 cctgcgtgaa ccgagccagc atttgcggcg tcagtgtcgg caggtgctgc atgaagcggc
     1321 gatagcggct ttgctcggac atgccggcga cgaacgcgca ctcgagatca gcatcctcgg
     1381 gccgtatcgg gcgaagcttc agcgcttctc cggaacgcaa atgcaagctc gtctccagct
     1441 ccaccggata ggggtgaatc gccatgtggc ggtagccgtc cgctagtcgg atgcgcgaag
     1501 ggtcgacgtt gacgctggca cgcgcgacga ccgcggcgat cgggctgacg acgaccgggt
     1561 cgagc
//
LOCUS       DATANS010000522         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_489193, whole genome shotgun sequence.
ACCESSION   DATANS010000522 DATANS010000000
VERSION     DATANS010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489193"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2500)
                     /locus_tag="VHT22_07670"
     CDS             complement(<1..2500)
                     /locus_tag="VHT22_07670"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEX3137471.1"
                     /translation="MVAIAEARIGNGTLRISDEAVSPAFKVTLTNVALDGRKIASNAG
                     LGTLDAAFDSDEGAHFGAHADIDLAGKSARGHFSLTGFHLAKLYPYYAEALNLDVRRG
                     TLDLTGDFDTAAAKQPMQFTLAQGGATLNDLEMAVRGERDPLWRIPRMDLNGVAFDLA
                     KRSVTIERTECRQAAIRVVRQADGVVNFARLVRTTGSTSTRDAGSRPSSTPDAGWSVV
                     TRKLLLERVTADVEDRVQEPAVKLRVNVASLSGDDISNERNAKGTIDATAKIGTGGRL
                     HMRGALATNPVNADLRIDANGIDLVPLRPYFEAQTNIIVTSGAVAAKGRVIYASTGPE
                     SPRASYAGDLTVSDFDSLDRPTSQELVRWKTLTLTGVDAASAPFKIALGAVALDQFYA
                     RLILDSDATLNLHRLLAPDAATPAPAPTVTSTLSGTATAELPPPRSDKQELPVSIGRI
                     QVSKGEVQFSDFFVKPNYSAHLTNVSGSVSALSATQAGDVDISASVEGTAPVQIGGTV
                     NPFARELALDLTAKARDIDLPPLTPYSVKYAGYGIQKGKLSLEVHYRIDNRKLAATNK
                     LVLDQLTFGEHVESPTATKLPVLLVLALLKDRNGVINLDLPIQGTLDDPKFSIWGVIV
                     QIVANLFTKAATAPFALLGALGGRGGEQLAYVEFAPGRADLSSPEEEKLGSLAKALTD
                     RPGLKLDAAGRAVADIDREGLKRVALDRAVRAQKQKALVAEGASAPSLDALTVDAAEY
                     PKYLAAVYRDTKLPDKPRNVLGFAKEIPPAEMEALLLASYGADEEALRGLANRRAQTV
                     KEWLVEKGSVPSERIFVVAAKLSGEGITDKGAVTR"
BASE COUNT          412 a          822 c          817 g          473 t
ORIGIN      
        1 cacgggtcac cgcgcctttg tcggtaatgc cttcacccga gagcttggcc gccacgacga
       61 agattcgctc cgacggcacg ctgcccttct cgactagcca ttcctttacc gtttgcgcgc
      121 ggcgattggc cagcccgcgc aacgcttctt cgtcggcacc gtagctcgcc agcagcagcg
      181 cctccatttc cgccggtggg atttccttgg cgaagcccag gacattgcgg ggcttatccg
      241 gcagcttggt atcgcgatac accgcagcca ggtacttcgg gtattcggct gcatcgaccg
      301 tcaaggcatc gagcgacggc gccgacgcgc cttcggcgac caaagccttt tgcttctggg
      361 cgcgcacggc ccggtcgagc gcgacgcgct tcagtccttc gcgatcgatg tcggccaccg
      421 cgcgtcccgc ggcgtcgagc ttcagtcccg gccggtcggt gagcgctttc gccagcgacc
      481 cgagcttctc ttcctccggt gacgacaagt ccgcgcgacc cggcgcgaac tcgacatagg
      541 ccagttgttc gccacccctg ccgcccaatg cgccgagcaa cgcgaaaggc gcggtggccg
      601 ccttggtgaa aaggttggcc acaatctgga cgatcacgcc ccagatcgag aacttggggt
      661 cgtcgagcgt gccttggatc ggcagatcga gattgatcac gccgttgcga tccttcagca
      721 gcgccagcac cagcagcacc ggaagcttcg tcgcggtcgg actctcgacg tgctcgccaa
      781 aggtaagctg gtcgagtacg agcttgttgg ttgcggcgag cttccggttg tcgatccggt
      841 aatgaacctc gagcgacagc ttgcctttct gaatcccgta gcccgcatat ttgaccgaat
      901 agggcgtgag cggcggcagg tcgatgtcgc gggccttggc ggtgaggtcc agcgcgagct
      961 cgcgcgcgaa agggttgacc gtgccgccga tctgcacagg cgcggtgccc tcgacgctcg
     1021 ccgagatgtc gacgtcgccc gcctgcgtcg ccgagagcgc tgaaacgctg cccgagacgt
     1081 tcgtaagatg cgcggaataa ttgggtttga cgaaaaagtc cgaaaactgc acctctccct
     1141 tcgacacctg gatgcggccg atcgacaccg gcaattcctg tttatcgctt cggggaggcg
     1201 gcaactcggc agttgcggtt ccggataacg tcgaagtcac cgtcggtgcc ggggcgggcg
     1261 tcgcagcgtc cggggcgagc agccggtgca ggttgagcgt cgcgtccgaa tccaggatga
     1321 gtcgcgcgta gaactgatcc agggcgacgg cgccgagcgc aatcttgaac ggtgccgacg
     1381 ctgcgtcgac gccggtgagg gtcaacgtct tccagcgcac gagttcctgc gaggtcggac
     1441 gatcgagcga atcgaaatcg ctgaccgtca ggtcgccggc atagcttgcc cgcgggcttt
     1501 cggggccggt ggatgcgtag atcacccgtc ccttggcggc gacggcgccg ctggtgacga
     1561 tgatgttcgt ttgcgcctcg aaatacggcc gcagcggcac cagatcgatg ccgttcgcat
     1621 cgatgcgcaa atcggcgttg acagggttgg ttgcgagtgc gccacgcata tgcagtcgcc
     1681 cgcccgtgcc gattttcgcg gtggcgtcga tcgtgccctt ggcgtttcgc tcattgctga
     1741 tgtcgtctcc cgagaggctc gcgacgttga cccggagttt cacggccggc tcctgcaccc
     1801 ggtcctcgac gtccgctgtc acgcgttcga gcagcaactt gcgggtgaca acgctccagc
     1861 ccgcatcggg ggtgctcgag ggccgcgagc cggcgtcgcg cgtgctggtg ctaccggttg
     1921 tgcgcaccag tcgcgcgaaa ttgacgacgc cgtccgcttg tcgcacgacc cggattgcag
     1981 cctgccggca ctcggtccgt tcgatcgtca cgcttcgctt cgccaagtcg aacgccacgc
     2041 cgttcaggtc catccgcgga atgcgccaca aggggtcgcg ctcgccgcgg accgccatct
     2101 cgagatcatt gagcgtcgcg ccaccctgtg cgagcgtgaa ttgcatcggt tgcttcgccg
     2161 ccgcagtatc gaaatctccc gtcaggtcga gcgtgccccg gcgaacgtcg aggttcagcg
     2221 cctcggcgta gtacgggtac agctttgcca ggtgaaaccc ggtcagcgaa aaatggccgc
     2281 gggccgattt ccccgccaga tcgatatcgg catgggcgcc aaaatgcgcg ccctcgtccg
     2341 agtcgaaggc cgcgtccaaa gtgcccaggc cggcgttcga tgcgatcttc ctgccgtcga
     2401 gcgccacgtt ggtcaaagtc actttgaacg caggtgagac cgcttcatca gagatccgca
     2461 gcgtgccatt gccgatccgg gcttcggcga tcgcgacaat ccacggcggg gcgggtggac
     2521 tggc
//
LOCUS       DATANS010000523         1780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_25636, whole genome shotgun sequence.
ACCESSION   DATANS010000523 DATANS010000000
VERSION     DATANS010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25636"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1499
                     /locus_tag="VHT22_07675"
     CDS             <1..1499
                     /locus_tag="VHT22_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX3137472.1"
                     /translation="SRVLSVSSTTTLAILAGTELGLVVPDGDPAKLVAATATLTALVG
                     AILILARMMRLGFVANFISAPVLTGFKAGIGLVIVLDQVPKLLGIHITKEGFFVDLLR
                     VVQHIPETSLMTLAVAAATFVVLIGMERLWPRAPAPLIALGGGIAAAWFFGLQALGVS
                     TVGLIPQGFPSLTIPDPALIGQLLPGALGIALMSFTETIAVGRAFARPGDPRIDANRE
                     LIATGAANLGGALLGAMPGGGGASQTAVVRAAGGRSQLASLVTAGVALATMLILAPLL
                     GLLPHATLAAIVIVYSVGLIQPAEFRAIRKVRTMEFRWAILAALGVLMLGTLKGIVVA
                     IILSLIGLASQSANPRVHVIGRKRGADVLRPLSPEHPDDETFEGLLIVRPEGRLYFVN
                     AQNVAEKIDALVMQYKPRVLALDMSRVQDIEYSALQAMMDGETRATGRGAIVWLAGLN
                     PGVLEVVRNSGLAERLGSERLLFNARAVIERFQALQRAAGGAAQSALF"
BASE COUNT          291 a          552 c          618 g          319 t
ORIGIN      
        1 cgtcgcgcgt gcttagcgtc agctctacca ccacgctggc gatccttgcc ggcaccgagc
       61 tgggcttggt tgtgcccgac ggcgacccgg cgaagcttgt cgcggcgacg gcgaccctca
      121 ccgcgctggt cggtgccata ctgattctgg cgagaatgat gcgactggga ttcgtcgcca
      181 acttcatttc cgcgccggtg ctgaccggct tcaaggccgg aatcggactc gtgatcgtcc
      241 tggaccaggt gccgaagctc cttggcatcc acattaccaa ggaggggttt ttcgtcgact
      301 tgctgcgagt cgtgcaacat attccggaga cctcgctgat gacactcgcg gtggccgccg
      361 cgactttcgt cgtgctgatc gggatggagc gcctttggcc gcgtgctcca gcgccgctga
      421 ttgcactcgg cggcggcatc gccgcagcct ggttcttcgg cctgcaagcg ctgggcgtct
      481 cgacggtcgg cctgattccg caagggttcc cgtcactcac catacccgac ccggcgttga
      541 tcgggcagtt gctgcccggc gcgctgggga tcgcgctgat gagtttcacc gaaacgatcg
      601 cggtgggtcg cgcttttgcc cgccccggag atccccgcat cgatgcgaat cgcgagttga
      661 tcgcgaccgg ggctgccaat ctcggcggcg cgctgctcgg cgccatgccg ggcggcggcg
      721 gcgcctcaca gaccgcagtc gtacgcgcgg cgggcggacg gtcgcaactg gcgtcgctcg
      781 ttaccgccgg cgtcgcgctc gccacgatgc tgattcttgc gccgttgctc ggtttgctgc
      841 cccacgccac gctcgcggcg atcgtcatcg tctactcggt gggactgatc cagccagcgg
      901 aattccgcgc cattcgaaaa gtgcgcacca tggagttccg ctgggcgatc ttggccgcac
      961 tcggcgtgct catgctcggc acgctcaagg gcatcgtggt ggcgatcatc ctgtcgctga
     1021 tcggtctggc cagccaaagt gccaacccgc gtgtgcacgt catcggccgc aagcgcggcg
     1081 ccgatgtgct gcgaccgttg tcgccggagc acccggacga tgagacgttc gagggcctgc
     1141 tgatcgtgcg gccggaggga cgcctctact tcgtcaacgc ccagaatgtg gcggaaaaga
     1201 tcgacgcgct ggtcatgcag tacaagccgc gcgtgctcgc gctggacatg agccgggtgc
     1261 aagacatcga atactcggcg ctccaggcga tgatggacgg cgagacccgc gcgaccgggc
     1321 gtggcgccat tgtatggctg gcaggcttga atcccggcgt cctggaggtc gtccggaatt
     1381 ccgggctggc cgaacgactg ggcagcgagc gcctgctgtt caacgcgcgt gcggtgatcg
     1441 agcgcttcca ggcgctgcag cgagctgcgg gcggcgcggc gcagtctgct ctcttttgac
     1501 cgctcgtgtg aggcggcgca cggcgaccgc cggtggcaca gcgaaattgt gcgaggatgc
     1561 gaagcggtgc aacggattca ttcgcggtga ctgacgaaca ctcgtagcat taattctctg
     1621 gggacgacga tggcaaagaa aagcgcagca aggaagggcg agccgaagag caaggagaag
     1681 aagggcgatg gggctgctgt caaagcgcgt gcggagaaaa ggcgcgcagc gccgcccaag
     1741 ggggagattt cggggaagga ctacgcgcgg gagatgaaga
//
LOCUS       DATANS010000524         1938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_572469, whole genome shotgun sequence.
ACCESSION   DATANS010000524 DATANS010000000
VERSION     DATANS010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572469"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VHT22_07680"
     CDS             <1..255
                     /locus_tag="VHT22_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEX3137473.1"
                     /translation="GEDALGAALAGLKLAGTTVVLVSHRPGLMRHADTLAILRNGALD
                     IAGPRDEVLARLNGQTVHPLRCAAPGLAAAIQHTAQGAQA"
     gene            252..1604
                     /locus_tag="VHT22_07685"
     CDS             252..1604
                     /locus_tag="VHT22_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEX3137474.1"
                     /translation="MSTHSLPAAAATSTTFASLLACSQRSAEQELALHQTLRHLCMPL
                     IAAAVLIALWCVWAPLSGAVVASGQVQAALGRKVVQHQEGGIVRELRVVQGQAVKQGD
                     ALLVVGDVRSDAQLDLLNKQMRDERLRAARSGAELALAMSFAVPADVAVTPELLARER
                     QLFDSRRRTLNEQLAAMQSQAHEAQSRIVALNAQLQAAQRSTALTRDELGINAELVAS
                     GFMQKTRLLTLERSVADNEARAAATRGQIAEAQMQVGALGNAMAQARGAYQQRAADEH
                     KDAVARVRELEDRLRPSQDQVDRQIVRAPVDGTVMALRIGAAGTVVGPREPLLEIAPS
                     EERLIVELRIDTHDIEHVRVGDAAEVRLSAFDTRRTPLLRATVTAVSPDAVSDTAGKQ
                     SWYAAQVEVERSELAGNPQLNLQAGMPAEVFVTTPPRSLLQYLLEPLGIFARRALREP
                     "
     gene            1719..>1938
                     /locus_tag="VHT22_07690"
     CDS             1719..>1938
                     /locus_tag="VHT22_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig family protein"
                     /protein_id="HEX3137475.1"
                     /translation="MAIITITGDNNANVLTGTSATDQYNINGLGGNDNMHGNALNDTL
                     DGGAGDDHMGGGAGSDVYIVDSTNDFVHE"
BASE COUNT          340 a          667 c          663 g          268 t
ORIGIN      
        1 ggcgaagacg cgcttggcgc ggcgctggcc gggctcaagc tggccggcac cacggtggtg
       61 ctggtatcgc accgtcccgg cttgatgcgc catgccgaca ccctggccat cctgcgcaat
      121 ggcgcgctcg acatcgccgg cccgcgcgat gaagtgctgg cgcggctgaa cggccagacc
      181 gtgcacccgc tgcgctgcgc cgcgccgggt ctcgcagccg ccatccagca caccgcccaa
      241 ggagcacaag catgagcacc cattccctgc cggcggccgc ggccaccagc acgacattcg
      301 ccagcctgct ggcgtgcagc cagcgcagcg ccgaacagga gctggcgctg catcagacgc
      361 tgcgccacct gtgcatgccg ctgatcgccg cggccgtgct gatcgcgctg tggtgtgtct
      421 gggcgccact gtcgggcgcg gtggtggcca gcggccaggt gcaggctgcg ctgggccgca
      481 aggtggtgca acaccaggaa ggcggcatcg tgcgtgagct gcgggtggtg cagggccagg
      541 ccgtgaagca gggcgacgcg ctcctggtgg tcggcgatgt gcgcagcgac gcccagctgg
      601 atctgctgaa caagcagatg cgcgacgagc ggctgcgggc tgcacgcagt ggcgccgagc
      661 tggcgctggc gatgtccttt gccgtgccag cggacgtggc tgtgacgcct gagcttctgg
      721 cgcgcgagcg gcagctcttc gactcgcgcc ggcgcacgtt gaacgaacag cttgccgcga
      781 tgcagtcgca ggcccacgaa gcacagtcgc gcatcgtcgc gctaaatgct cagctgcagg
      841 ccgctcaacg atcgaccgca ctgacgcgcg acgaactcgg catcaacgcg gagctggtgg
      901 ccagcggctt catgcagaag acccggctgc tgacgctgga gcgcagcgtc gccgacaacg
      961 aagcccgggc cgccgcaacc cgcggccaaa tcgccgaggc gcagatgcag gtaggcgcgc
     1021 tgggcaatgc gatggcacag gcgcgcggcg cctaccagca gcgcgccgcc gacgagcaca
     1081 aggatgccgt cgcacgggta cgcgaactcg aagaccggct gcgcccgtcg caggaccagg
     1141 tcgaccggca gatcgtgcgc gcgccggtgg atggcacggt gatggcactg cgcatcggcg
     1201 ccgccggtac ggtcgtgggg ccgcgtgagc cgctgctgga aatcgcgccg tccgaagagc
     1261 ggttgatcgt cgagctgcgc atcgacaccc acgacatcga acacgtgcgc gtcggcgacg
     1321 ccgccgaagt gcgcctgtcg gccttcgaca cgcgccgcac gccgctgctg cgcgccacag
     1381 tgacggcggt atcgcccgac gcggtgagcg acaccgccgg caagcagtcc tggtatgccg
     1441 cgcaggtcga ggtcgagcgc agcgaactgg cgggcaaccc gcaactgaat ctccaggcgg
     1501 gcatgccggc cgaggtcttt gtgacgacgc cgccgcgcag cctgctgcag tacctgctcg
     1561 agccgctggg catcttcgcg cgccgcgcgc tgcgcgagcc ctgagcggcg agaaccacag
     1621 atcccattcg agaaaggagg tgccgaacac gcgacccaca aggaccgttt cctgagtttt
     1681 cttttcgact ttcacttgac taccggagct tcatcatcat ggccatcatt accatcactg
     1741 gcgacaacaa cgccaatgtg ctgaccggca catcggctac cgaccaatac aacatcaacg
     1801 gtctgggcgg caacgacaac atgcatggaa acgccctcaa tgacacactc gacggcggtg
     1861 ccggcgacga ccacatgggc ggcggcgccg gcagcgacgt ctacatcgtc gactcgacca
     1921 acgacttcgt tcacgagg
//
LOCUS       DATANS010000525         1507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_109824, whole genome shotgun sequence.
ACCESSION   DATANS010000525 DATANS010000000
VERSION     DATANS010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109824"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VHT22_07695"
     CDS             complement(<1..215)
                     /locus_tag="VHT22_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137476.1"
                     /translation="MQTIDHVVAGKQAWRRADIRRDDWFFPLTPDCLAELRAIGLAAR
                     ANPRPVEQIDPAQFTLTACRAFMRRVQT"
     gene            complement(238..1116)
                     /locus_tag="VHT22_07700"
     CDS             complement(238..1116)
                     /locus_tag="VHT22_07700"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX3137477.1"
                     /translation="MMAVTFKKLHPHIGAEVSPLDLRQVHDRATLDALVAGMDEHGVL
                     VFRDQPFTDAEQMAFAQRFGGPLHSKPGIAALGKNRFNSDAIADISNVDEEGRIRKAD
                     DRRRAYSLANRLWHTDASFQDPPGRYSTLSARVLPPVGADTEFADMQAAYDALDAATK
                     KQIEGLHVHHSIAYSRQLLGFEFSAEELERLKGAVHPLVHVNPRTQRKSLYLASHATR
                     VIEMLVPEGRLLLRDLTEHATQRQFVYRHQWRERDLVIWDNQASMHRSTPFDDAHHRR
                     ELRRITTIDVEVPAYA"
     gene            complement(1173..>1507)
                     /locus_tag="VHT22_07705"
     CDS             complement(1173..>1507)
                     /locus_tag="VHT22_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX3137478.1"
                     /translation="AGNADTLEEGERLANLIGDKQVMFMKNHGVLVVGETVPEAYRRL
                     YMLERVCRAQVLAMSTGRELEIISDKIVAKVHTPMQSDRHSRADRERMYFDAMMRVLD
                     RELPGYAE"
BASE COUNT          225 a          461 c          530 g          291 t
ORIGIN      
        1 gtttgcacgc ggcgcatgaa cgcgcggcac gcggtcagcg tgaattgcgc ggggtcgatc
       61 tgctcgacgg ggcgcggatt ggcgcgcgcg gcgaggccga tcgcgcgcag ttcggcgagg
      121 caatcgggcg tgagcgggaa aaaccagtcg tcgcgccgga tgtcggcgcg ccgccacgct
      181 tgcttgccgg cgacgacgtg atcgatggtc tgcatcggct gagtgcgcgc tgacgcgtca
      241 ggcgtacgcg ggaacctcga cgtcgatggt cgtgatgcgg cgcagttcgc gccggtggtg
      301 cgcgtcgtcg aacggcgtcg agcgatgcat gctcgcctgg ttgtcccaga tcacgagatc
      361 gcgctcgcgc cattggtggc ggtacacgaa ttggcgctga gtcgcgtgtt cggtcaggtc
      421 gcgcagcagg aggcggcctt cggggacgag catctcgatc acgcgcgtcg cgtgcgacgc
      481 gaggtacagc gatttgcgct gcgtgcgcgg attgacgtgt acgagcggat gcaccgcgcc
      541 tttcagccgt tcgagctctt cagcggaaaa ttcgaagccg agcagctggc gcgagtaggc
      601 gatcgagtgg tgcacgtgca acccttcgat ctgctttttg gtcgcggcgt cgagcgcgtc
      661 gtacgcggcc tgcatgtcgg cgaattcagt gtcggcaccg acgggtggca gcacccgtgc
      721 cgacagcgtc gaataccggc cgggcggatc ctggaacgac gcgtcggtgt gccacaggcg
      781 gttggcgagc gaatacgcgc ggcgccggtc gtcggccttg cggatgcggc cttcttcgtc
      841 gacattcgaa atgtccgcga tcgcgtcgct gttaaagcgg ttcttgccga gcgcggcaat
      901 gccgggcttg gaatgcagcg gaccgccgaa tcgctgcgcg aacgccatct gttcggcgtc
      961 ggtgaatggc tggtcgcgaa acaccagcac gccgtgttcg tccatgcccg cgaccagcgc
     1021 gtccagcgtg gcgcggtcgt ggacctggcg caggtcgagc gggctcactt ccgcgccgat
     1081 atgcgggtgc agctttttga atgtgacggc catcatcggt ctcctcggcg ggaacgctgc
     1141 ccgccagcat tgatttcgaa tgcctgtgtt atctattcag cgtagcccgg caactcgcgg
     1201 tcgagcacgc gcatcatcgc gtcgaagtac atgcgctcgc ggtcggcgcg cgagtggcgg
     1261 tcgctttgca tcggggtatg caccttggcg acgattttgt cggagatgat ttcgagctcg
     1321 cgcccggtgc tcatcgccag cacttgcgcg cggcatacgc gctccagcat gtacagccgc
     1381 cgataggctt caggcaccgt ctcgccgacc accagcacgc cgtggttctt catgaacatg
     1441 acctgcttgt cgccgatcag gttggccagg cgctcgcctt cttcgagtgt gtccgcgttg
     1501 cccgcat
//
LOCUS       DATANS010000526         1600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_489339, whole genome shotgun sequence.
ACCESSION   DATANS010000526 DATANS010000000
VERSION     DATANS010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489339"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VHT22_07710"
     CDS             <1..580
                     /locus_tag="VHT22_07710"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HEX3137479.1"
                     /translation="KAYLALYSLVAFATLGWMIWAFYHAPFVNLWYAVALRPVPLIVM
                     PFALVLIVCALATPNPTLVGRARLLKSADAARGILRITRHPLMWGFALWALSHIVARG
                     DAAAAIFFGTFVVLAVSGTWLIDRRKAATLGDNWRRFAAVTSNVPFAAIAAGRNRFRL
                     GEIGWWKIALGLVLYAVLLVFHHRFFGAHALI"
     gene            647..1279
                     /gene="pdxH"
                     /locus_tag="VHT22_07715"
     CDS             647..1279
                     /gene="pdxH"
                     /locus_tag="VHT22_07715"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX3137480.1"
                     /translation="MKIADLRQEYMRESLDEHDVARDPLVQFNRWFDEAVAAKMPMLN
                     SMTLATVSAAGQPSARIVLLKGVDARGFVFFTNYASRKGVDLAANPAAALLFHWVELE
                     REVRIEGRVEKVSARESDDYFASRPLGSRHAAIASPQSEVVPDRAALEKSFAAAEKSH
                     GSAPVRPPQWGGYRVQPSSLEFWQGRPNRLHDRVLYTRVGDSWKIERLAP"
BASE COUNT          268 a          501 c          493 g          338 t
ORIGIN      
        1 caaggcctac ctcgcgcttt attcgctggt cgccttcgcg acgctcgggt ggatgatctg
       61 ggcgttctat cacgcgccgt tcgtgaacct gtggtacgcc gtggcgctgc ggccggtgcc
      121 gctcatcgtg atgccgttcg cgctggtgtt gatcgtgtgc gcgctggcga cgccgaatcc
      181 gacgctggtc gggcgtgcac gcctgctcaa atccgccgac gcggcgcgcg gcatattgcg
      241 gatcacgcgc catccgctga tgtggggttt cgcgttgtgg gccttaagtc atatcgtggc
      301 gcgcggcgat gctgcggccg cgatcttttt cggcacgttc gtcgttcttg cagtgtccgg
      361 cacgtggctc atcgaccggc gcaaagccgc cacgctcggc gacaattggc gtcgctttgc
      421 cgcggtcacg tcgaacgtgc cgttcgcggc gatcgccgct ggccgcaacc ggtttcgcct
      481 cggcgaaatc ggctggtgga aaattgcgct gggactcgtc ttgtatgcag tcctgctcgt
      541 gtttcatcac cgcttcttcg gcgcgcacgc gctcatctga gcccggcgcc gcgcccgctc
      601 cccgcagtgc ggctaaaata gtgccgttgc tctcccggta aaactcatga aaatcgccga
      661 tctgcgccag gaatacatgc gtgaaagtct ggacgagcac gacgtcgcgc gcgatccgct
      721 ggtgcaattc aatcgctggt tcgatgaagc cgtcgccgcg aagatgccga tgctgaactc
      781 gatgacgctc gccaccgtgt cggcagccgg tcagccgtcg gcccgcatcg tgctcctgaa
      841 aggcgtcgat gcgcgcggtt tcgtcttctt caccaactat gcatcgcgca aaggcgtgga
      901 tcttgcggcg aatcccgccg cggcgctgct tttccactgg gtcgagctcg aacgcgaagt
      961 gcgcattgaa ggccgcgtcg aaaaagtgtc ggcgcgcgaa tccgacgatt acttcgccag
     1021 ccgcccgctc ggcagccggc acgccgcgat agcatcgccg caaagcgagg tcgtgcctga
     1081 ccgcgcggcg ctcgaaaaaa gtttcgcggc agcggaaaaa tcacatggat ccgcgcccgt
     1141 gcgtccgccg caatggggcg gctatcgggt gcagccttcg tcgctcgaat tctggcaggg
     1201 ccggcccaac cggctgcacg atcgcgtgct ttatacgcga gtgggcgaca gctggaagat
     1261 cgaacgtctc gcgccttagg tgccgctgtt ctacggcgat cgctcgccgc tttgcagccc
     1321 ctctcgccga atgggttgcg caactcgtgt gttgcaccaa ctgcgagcgc aatgtctttg
     1381 ttgcacaaaa taatttcgcc tgcgaattag ccattattgg tgcatcgcaa caatttatga
     1441 acaggcgcgg tgcgaaatta tagttttgcg gcccggaact aaacattaag ccattgaatt
     1501 aaagacgaga gctgacctgg catcgtcctt gcgaccctcc ccgcatgagc ctcagtcacg
     1561 tttcggtgca gggtcgctct gacgcggcaa tattgcagcg
//
LOCUS       DATANS010000527         2478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_196498, whole genome shotgun sequence.
ACCESSION   DATANS010000527 DATANS010000000
VERSION     DATANS010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196498"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VHT22_07720"
     CDS             complement(<1..323)
                     /locus_tag="VHT22_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137481.1"
                     /translation="MSDTLSRFRTGRRIFLATLLLCGLFAAPLPAADAPDDSLAGPAL
                     LSALRAGGLILYFRHTSTDFGQNDDQMSGYEDCARQRNLTSRGRDEARAIGAAIQRLR
                     IPIGEV"
     gene            complement(346..1326)
                     /gene="ttcA"
                     /locus_tag="VHT22_07725"
     CDS             complement(346..1326)
                     /gene="ttcA"
                     /locus_tag="VHT22_07725"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEX3137482.1"
                     /translation="MTDSTAAPAASPETTFPTLLEHADTAPAATSSARQAQRQAFEAN
                     KLKKRLRRLVGQAIGDFSMIGRGDRVMVCLSGGKDSYAMLDVLLSLRDRAPLPFELIA
                     VNLDQKQPGFPADVLPRYLASRGVPFHIVEQDTYSVVKRVIPEGGTMCSLCSRLRRGV
                     LYRVAGELGATRIALGHHRDDILATFFLNLFFGGKLKTMPPKLVSDDGKHVVIRPLAY
                     VRERDLARYAEVMAFPIIPCTLCGSQENLQRKQVAAMLCDWQKRYPGRAESIFNALSN
                     VVTTHLLDRSLQDFAAIRASGMAVPDGDIAFDDDPCIDAGARSVRFAPGD"
     gene            1454..2098
                     /locus_tag="VHT22_07730"
     CDS             1454..2098
                     /locus_tag="VHT22_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX3137483.1"
                     /translation="MQTLLVANPKGGSGKTTLATNIAGWLAAKKQRVVLADFDPLRSA
                     TEWLARRPPLFPAISGWTPNAGKEDLKEHHPQWLVLDTPAGLHGDALRDAVRRADILL
                     VPVSPSAFDTAATQHFLDQIGEYKAIKKGDIAVGLVAMRVDPRTNSAAELEEFLKSFD
                     FPLVAHLRATQVYVHCARDGLTIADLPHSRAEQDLEQWRPLTRWIARHSTAKNG"
     gene            complement(2123..>2478)
                     /locus_tag="VHT22_07735"
     CDS             complement(2123..>2478)
                     /locus_tag="VHT22_07735"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX3137484.1"
                     /translation="ESVHTTLFYSALVGMLGLSLVLPWEIGAAAPTLKDTVLFVLLGL
                     FAGVGHWSIIGAFQHAPAALLAPFAYLQMIWATSYGFVLFGQLPDRWSALGMTIIVAS
                     GLVLALRERARAPRR"
BASE COUNT          465 a          787 c          837 g          389 t
ORIGIN      
        1 acttcgccga ttggaatgcg cagccgttga atcgccgcac cgatcgcccg cgcctcgtcg
       61 cggccgcggc tggtcaggtt gcgctggcgc gcgcaatctt cgtagccgct catctggtcg
      121 tcgttctggc cgaaatcggt cgaggtgtgg cggaaataga ggatcaatcc gccggcgcgc
      181 aaggcggaga gcagcgccgg cccggcgagc gaatcatctg gcgcgtcggc tgccggcaga
      241 ggtgccgcga acaacccgca caagagcaag gtcgcgagaa agatccttcg tccggtccgg
      301 aatcgcgaaa gcgtgtcgct catagaggtg cggggcgggg gtgcgtcagt cgcccggcgc
      361 gaagcgcacg ctgcgggcgc ccgcgtcgat gcagggatca tcgtcgaatg cgatatcgcc
      421 gtccggcacc gccatgccgc tggcgcggat cgccgcgaaa tcctgcaggc tgcgatcgag
      481 cagatgcgtg gtcacgacgt tcgacagcgc gttgaaaatg ctctcggcgc ggcccggata
      541 ccgcttctgc cagtcgcaca gcatcgcagc gacctgcttg cgctgcaggt tctcctgcga
      601 gccgcacagc gtgcatggaa tgatcgggaa cgccatcacc tcggcatagc gcgcgagatc
      661 gcgctcgcgc acgtaagcga gcggacggat gacgacgtgt ttgccgtcgt cggacaccag
      721 cttcggcggc atcgtcttca gctttccgcc aaaaaacaga ttcagaaaga aggtcgccag
      781 gatgtcgtcg cgatgatggc cgagagcgat ccgggtcgca ccgagctcgc cggcgacccg
      841 gtatagcacg ccgcgccgca atcgcgagca caacgagcac atcgttccgc cttcgggaat
      901 cacgcgcttg acgacactgt aggtatcctg ctcgacgatg tggaacggca cgccgcgaga
      961 cgcgagatag cgcggcagca cgtcggcggg gaagcccggc tgtttctggt cgaggttgac
     1021 cgcgatcaat tcgaacggca gcggcgcacg atcgcgcaag ctgagcagga cgtcgagcat
     1081 cgcgtagctg tccttgccgc cggacaggca caccatcacg cggtcgccgc gaccgatcat
     1141 cgaaaaatcg ccgatcgcct gtccgaccag ccggcgcagg cgctttttga gcttgttggc
     1201 ttcgaacgcc tgacgctggg cctgacgtgc gctggaagtc gccgcgggcg ccgtgtctgc
     1261 gtgctccagc agggtcggga acgtcgtctc gggcgaggcg gccggcgcgg cagtcgagtc
     1321 ggtcatcgcg gtgtcgggaa tcattgcgcc aacgtagcgc gagtcgcgct cgaacgcaac
     1381 ccgctctaca atgcagggct ttaaagccag gccgaccggc cggatcgtct cgggggaggg
     1441 gaacgcgatg gcaatgcaga cgctactggt ggccaatccg aagggcggca gcggcaagac
     1501 gacgctggcc accaacatcg ccggctggct cgcggcaaag aagcagcgtg tcgtgctggc
     1561 cgacttcgat ccactgcgtt cggcgaccga gtggctggct cgccgcccgc cactgtttcc
     1621 ggcgatttcg ggctggacgc cgaatgcggg aaaggaagat ctgaaggaac atcatccgca
     1681 gtggctggtg ctcgatacgc cagcaggcct gcatggcgat gcgctgcgcg atgcggtgcg
     1741 ccgcgccgac atcctgctgg tgcctgtgtc gccgagcgcg ttcgacaccg ccgcgaccca
     1801 gcacttcctc gatcagatcg gcgaatacaa ggcgatcaag aaaggcgata tcgccgtggg
     1861 gctggtcgcg atgcgcgtcg atccgcgcac gaatagcgcg gcggagctcg aggagtttct
     1921 gaagtcgttc gactttccgc tggttgctca ccttcgcgca acacaggtct atgtgcattg
     1981 tgcccgcgac ggcttgacga tcgccgatct gccgcactcg cgcgccgagc aggatctcga
     2041 gcagtggcgg ccgctgacgc ggtggatcgc gcgccacagc acggcgaaaa acggctgatc
     2101 gcatagactt tacgcacggg atctatcgcc gcggtgcgcg agcccgttcc cgcagtgcga
     2161 gcaccaggcc gctggcaacg atgatcgtca tgcccagcgc cgaccagcga tcgggcagct
     2221 ggccgaacag cacgaagccg taactggtcg cccagatcat ctgcagatag gcgaaaggcg
     2281 ccagcagcgc ggcgggcgca tgctggaatg cgccgatgat cgaccagtgg ccgacgccgg
     2341 cgaaaaggcc gaggagcaca aacaacacgg tgtccttcaa cgtcggcgcg gcggcgccga
     2401 tctcccacgg aagcacgaga ctcaggccga gcatgccgac cagcgcgcta tagaacaagg
     2461 tcgtgtgcac actttcat
//
LOCUS       DATANS010000528         3200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_322783, whole genome shotgun sequence.
ACCESSION   DATANS010000528 DATANS010000000
VERSION     DATANS010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322783"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..953)
                     /gene="htpX"
                     /locus_tag="VHT22_07740"
     CDS             complement(87..953)
                     /gene="htpX"
                     /locus_tag="VHT22_07740"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX3137485.1"
                     /translation="MLGNWFKTGLLMAAIMALFGIAGAAIGGGQGMLIALLFGLGTNL
                     WAYWFSDAMVLRLYNAREVEATTAPQLYGAVRDLAARAGLPMPRVYLIDEAQPNAFAT
                     GRNPEHAAIAATRGIVELLSARELRGVLAHELAHVRNRDMLTSTITASIAGAISALAH
                     FGMFFGHRDDGERNPLMALLVMILAPIAAVLIQLAISRGREYEADRSGAEISGDPGAL
                     ADALAKMERYTKGVPLPPAEEHPATAQMMIVNPLHDGGLAGLFSTHPPIEERIRRLLA
                     LASQFQRGLQPR"
     gene            complement(1022..1963)
                     /gene="fmt"
                     /locus_tag="VHT22_07745"
     CDS             complement(1022..1963)
                     /gene="fmt"
                     /locus_tag="VHT22_07745"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX3137486.1"
                     /translation="MPDALRVAFAGTPPFAASALAAILDAGHDVALVLTQPDRPQGRG
                     LKLAPSAVKTLAVERGLTVLQPPTLKGLPPPTGARPDILIVAAYGLILPQAMLDWPRH
                     GAINIHASLLPRWRGAAPIQRALLAGDEETGISIMQMDPGLDTGPVIARTVVPIAPRD
                     TAGTLTARLTAVGAEAVVRTLAQLTKAGVLASQPQDAPAATYAGKIGRTEAAIRWNED
                     AVTIDRVVRALNPAPGAFATLQGATIKIWAAEPTEGFGPAGEILSADGNGLLVACGRG
                     ALRVRELQRGGGRRLSAASFLDGHPVAAGATFEGRSD"
     gene            complement(1988..2491)
                     /gene="def"
                     /locus_tag="VHT22_07750"
     CDS             complement(1988..2491)
                     /gene="def"
                     /locus_tag="VHT22_07750"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX3137487.1"
                     /translation="MMVLDILEYPDPRLRRHAAPVAAVTPEIRKLVADMAETMYAAPG
                     VGLAATQVDVHKRIIVIDVSEARNDLKVFINPTIVAAEGEAECEEGCLSVPGYYDNVT
                     RAARITVRAQDERGDPFELSTDGMLAVCIQHEMDHLVGKVFVDYLSPLKRARLAAKLK
                     KKQRLAG"
     gene            2599..>3200
                     /locus_tag="VHT22_07755"
     CDS             2599..>3200
                     /locus_tag="VHT22_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX3137488.1"
                     /translation="MSLNSRLNSIIATLLFSAVPLVLMLASPAAGAEDLQLQDSRPDR
                     YTVQKGDTLWGISGKFLKQPWRWPEIWRMNREQIKNPHWIYPGDVVVLDKVDGQWRLS
                     LNRTAGPRPDARLSPSIRVENLEGEAIPTIPAGDLAPYLSKPLIATTRDGFPGAARII
                     AAHDERVVRGEGDYVYAVDVEEKAGTQWLIYRPGKVLRSY"
BASE COUNT          533 a         1073 c         1062 g          532 t
ORIGIN      
        1 ccgctgcgcg cgctccatcg cggggcgcgc ggctagcgtt ctgtcccgca aattcgctcg
       61 atagtcgtaa cttgcggcac aggaccctag cgcggctgca atccgcgctg gaattgcgaa
      121 gccagtgcaa gcagacggcg gatgcgctcc tcgatcggcg ggtgcgtgct gaacagtccg
      181 gcgagtcctc cgtcatgcaa cggattgacg atcatcattt gcgcggttgc aggatgctct
      241 tcggcgggtg gcagcggcac gccctttgtg tagcgctcca ttttcgccag cgcatcggcc
      301 agcgcgcccg ggtcgccgga aatctcggcg ccgctgcgat cggcctcgta ttcgcgtccg
      361 cgggaaatgg cgagctggat aagcaccgcg gcgatcggag ccaggatcat caccagcaac
      421 gccatcagcg ggttgcgttc accatcgtcg cggtggccga agaacatgcc gaaatgcgcg
      481 agcgccgata tggcgccggc gatcgatgcg gtgatcgtcg aggtgagcat gtcacggttc
      541 cggacatggg ccagctcgtg cgcaagtacc ccgcggagct cgcgcgcgct cagcaattcg
      601 acgatgccgc gagtcgcggc gatcgcggcg tgctccggat tgcgaccggt ggcgaacgcg
      661 ttcggctgcg cctcgtcgat caggtagacc ctgggcatcg gcaggccggc gcgcgcggcc
      721 aagtcgcgca ccgcgccata gagctgcggt gcggtcgtcg cttcgacctc gcgagcgttg
      781 tagagccgta gcaccatcgc gtccgaaaac cagtacgccc aaagattggt gcccaggccg
      841 aacagcagcg cgataagcat tccctgcccg ccaccgatcg cggcgccggc gatgccaaac
      901 agcgccatga tggccgccat caacaaacct gtcttgaacc agttgcccaa catgatcgtc
      961 atctccgcct cgaaatcagc gaggccttca gcgccttaga tgcccgcgat gcgcccgtaa
     1021 ttcaatcgga tctcccctcg aaagtcgcgc cggccgcgac tggatgtccg tcgagaaacg
     1081 aggcggcgga cagccggcgg ccacccccgc gctgcagttc gcgcacccgt agcgcgccgc
     1141 ggccgcaggc gaccagcagg ccgttgccgt cggcgctgag aatctcgccg gccgggccga
     1201 atccttcggt cggctcggcc gcccagatct tgatcgtcgc gccctgcagc gtcgcaaacg
     1261 ccccgggggc cgggttaagg gcgcgaacga cacggtcgat cgtcaccgcg tcctcgttcc
     1321 agcgtatcgc ggcttcggtc cggccgatct tgcctgcata ggttgcggct ggcgcgtcct
     1381 ggggctgcga ggcgaggacg ccggctttcg tcaactgagc gagcgtccgg acgaccgcct
     1441 cggcacccac cgcggtcagc ctcgccgtca gcgtgcccgc ggtgtcgcgc ggtgcgatcg
     1501 gcacgaccgt gcgggcgatg accggaccgg tgtcgaggcc gggatccatc tgcatgatgc
     1561 tgatgccggt ctcctcgtct cccgccagca gcgcgcgctg gatcggcgcc gcgccgcgcc
     1621 agcgcggcaa cagcgaggcg tggatgttga tcgcaccgtg gcgcggccag tcgagcatgg
     1681 cctgcgggag tatcagcccg tacgcggcca cgatcagtat gtcgggtctt gcgccggtcg
     1741 gcggcggcag gcccttcagc gtcggcggtt gaagcactgt caggccgcgc tcgacggcca
     1801 gcgtcttgac tgcggacggc gcgagcttga ggccgcgccc ctgcggccgg tccggctgag
     1861 taagcaccag cgccacgtcg tggccggcgt ccagaatcgc cgccagcgcc gaggcggcga
     1921 acggtggggt tccggcgaaa gctacgcgca gcgcatcggg cacgcccgat tatggcctgt
     1981 gcgagcccta gcccgcgagg cgctgcttct tcttcagctt ggccgcaagc cgcgcccgct
     2041 tcagcggcga caggtagtcg acgaagacct tgccgaccag atgatccatt tcatgctgaa
     2101 tacatacggc gagcatcccg tcggtggaaa gctcgaatgg atcgcctcgc tcgtcctggg
     2161 cgcggacggt aatgcgcgcg gcccgcgtca cgttgtcgta atagccgggc accgacaggc
     2221 aaccctcttc gcactcggct tcgccctcgg cggcgacgat ggtcgggttg atgaaaacct
     2281 tgaggtcgtt gcgcgcctcg ctcacgtcga tgacgatgat ccgcttgtgc acgtcgacct
     2341 gggtggcggc caggcctacg cccggggcgg catacatggt ttcggccatg tcggcgacca
     2401 gtttacggat ttcgggggtc accgcggcga ccggggcggc gtgccgccgc agccggggat
     2461 cggggtactc caggatgtcc agaaccataa ttacttgcaa aatgaatact cgggatgcag
     2521 aatccgacct aaacccttat tttgcgtcgg tttaggacgt ttgcaagtcc cgcacaacac
     2581 ctgtaccgga gaggccccat gagcctaaac tcgcggctga attctatcat agcgaccctg
     2641 ctgttttcgg cggtcccgct cgtgctcatg ctcgcgtcgc cggccgccgg cgccgaagac
     2701 cttcagctgc aggacagccg gccggaccgg tacacagtgc aaaaaggcga cactctgtgg
     2761 ggcatctccg ggaagttcct gaagcagccg tggcgctggc ccgagatctg gcggatgaac
     2821 cgcgagcaga tcaagaatcc gcattggatc taccccggtg acgtcgtcgt gctcgacaag
     2881 gtcgacggcc aatggcggct ctcgctcaat cgcacggccg gcccccgccc ggacgctcgc
     2941 ctgtcaccgt cgatccgggt cgaaaacctc gaaggcgagg cgattccgac cataccggcc
     3001 ggcgacctcg ctccgtacct cagcaagccg ctcatcgcca cgacccgcga tggcttccca
     3061 ggcgcggccc ggatcatcgc ggcgcacgac gaacgcgtcg tgcgaggcga gggtgactac
     3121 gtttacgcgg tggacgtcga ggaaaaggcg ggaacgcagt ggcttatcta ccgtcctggc
     3181 aaggtgctgc gctcgtacga
//
LOCUS       DATANS010000529         1797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_235217, whole genome shotgun sequence.
ACCESSION   DATANS010000529 DATANS010000000
VERSION     DATANS010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235217"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..144
                     /locus_tag="VHT22_07760"
     CDS             22..144
                     /locus_tag="VHT22_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3137489.1"
                     /translation="MKKIGRVDDIAMACVYLAGDSGSYISGQVIHLNGGQFMSY"
     gene            153..593
                     /locus_tag="VHT22_07765"
     CDS             153..593
                     /locus_tag="VHT22_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX3137490.1"
                     /translation="MVEFKTISYDVVRVGEEFRSDDFLIKPEDVETFAYAVDDHHPWY
                     FDESPYGGPIAHPVLLGNQALMMRHSRYIIPAGLHAKIQFEFLEPIRAGMRVRSYGKV
                     IDKFEKRGRYYMVTEFATKDEESGAVLVRGQFTQNIFKGETHGA"
     gene            583..999
                     /locus_tag="VHT22_07770"
     CDS             583..999
                     /locus_tag="VHT22_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX3137491.1"
                     /translation="MGLKAADSIEIGHELAPRVKPIAQRQIDCYSGVRPKSIHTDAEW
                     AKKKGFAAPLAQGLMSTAYVSQMMVDFLGEGFVRGGKISASFIKPVLAGDTLTMHGAV
                     KSKEAEGPHTRVTVEFWCVSQRGEKTMVGTASGLAA"
     gene            1017..>1797
                     /locus_tag="VHT22_07775"
     CDS             1017..>1797
                     /locus_tag="VHT22_07775"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX3137492.1"
                     /translation="MDNGIPPALETDLTRLFAPRSVALVGATDHPTNFGGRVFRAMLN
                     FGYAGKVYPVNPRLKEIYGLACYPGIKELPETPDHVGIIVATERVFDILADCAARRVP
                     FATVYTAGFAETATPEGRERQARLIAFARASGMRIMGPNCNGVINFIDSFAMTSTGAI
                     GRARGAAGNVGVISHSGGLGQITVMWRAQMAGLGVSYEASCGNEADLDTLDFARFMLR
                     SDTTDIILMAIEGFKDGAKLEAVAREALEREKPIVVLKLGRT"
BASE COUNT          341 a          542 c          601 g          313 t
ORIGIN      
        1 atgcgtttgg gccagattcc gatgaagaaa atcggccgcg ttgacgatat cgcgatggcc
       61 tgcgtgtacc tggccggcga tagcgggtct tatatctcgg ggcaggtgat ccatctcaac
      121 ggcgggcagt tcatgtccta ctagaggcaa gcatggtcga gttcaagaca atttcgtatg
      181 acgtggtgcg ggtcggcgag gagttccgct ccgacgattt tctgatcaag ccggaagatg
      241 tcgaaacgtt cgcctatgcg gtcgacgatc accatccgtg gtatttcgac gagtcgccgt
      301 acggcgggcc gatcgcgcac ccggtgctgc tgggcaacca ggcgctgatg atgcgccaca
      361 gccgctacat cattccggcg ggcctgcacg ccaagatcca gttcgagttc ctcgaaccga
      421 tccgggcggg catgcgcgtg cgctcgtacg gcaaggtcat cgacaagttc gaaaagcgcg
      481 gtcgctatta catggtgacc gagttcgcga cgaaggacga ggagtccggc gccgtgctcg
      541 tgcgcggcca gttcacgcag aatattttca agggtgagac ccatggggct taaagcggcg
      601 gattccatcg aaatcgggca tgagctcgcg ccgcgcgtaa agccgatcgc gcagcggcag
      661 atcgattgct attcgggcgt gcgcccgaaa tccatccaca ccgacgccga gtgggccaag
      721 aaaaaaggct ttgcggcgcc gctcgcgcag ggcctgatgt cgaccgccta cgtgtcgcag
      781 atgatggtgg attttctggg cgagggattc gtgcgcggcg gcaaaatcag cgcgtcgttc
      841 atcaagccgg tgctcgcggg cgacacgctg accatgcacg gcgcggttaa aagcaaggaa
      901 gcggaaggcc cccatacgcg ggtcaccgtc gagttctggt gcgtcagcca gcgcggcgaa
      961 aaaaccatgg tcggcacagc gagcggactc gcagcctgac gccagtgaat gtatcagtgg
     1021 ataacgggat ccccccggcg ctggaaaccg acctcacgcg cctgtttgcg ccgcgctcgg
     1081 tcgcgctggt cggcgccacc gaccatccaa cgaatttcgg cggccgcgtg ttccgcgcga
     1141 tgctcaattt cggttatgcg ggcaaagtct atccggtcaa tccgcgactg aaggaaatct
     1201 acggcctcgc gtgctatccg ggcatcaagg aattgccgga gacgcccgac catgtcggca
     1261 tcatcgtcgc gaccgagcgc gtgttcgata tcctggcgga ctgcgccgcg cgccgcgtgc
     1321 cgttcgcgac cgtttacacc gcgggattcg cggagaccgc gacccccgag ggccgcgagc
     1381 ggcaggcgcg cctgattgcg ttcgcgcgcg cgtcgggcat gcgcatcatg gggccgaact
     1441 gcaacggcgt gattaatttc atcgacagct tcgcgatgac gtcgaccggc gcaatcggcc
     1501 gcgcgcgcgg cgccgcgggc aacgtcggag tgatcagcca cagcggcggg ctcggccaga
     1561 tcaccgtgat gtggcgcgcg caaatggcgg ggctcggcgt gagctatgag gcgagctgtg
     1621 gcaacgaagc cgacctcgac acgctcgact tcgcgcgctt catgctgcgc tccgatacga
     1681 ccgacatcat cctgatggcg atcgagggct tcaaggacgg cgccaagctc gaggcggttg
     1741 cgcgcgaagc gctcgagcgc gagaagccga tcgtggtgct gaagctggga cgcaccg
//
LOCUS       DATANS010000530         1991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_110056, whole genome shotgun sequence.
ACCESSION   DATANS010000530 DATANS010000000
VERSION     DATANS010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110056"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..>1991)
                     /locus_tag="VHT22_07780"
     CDS             complement(41..>1991)
                     /locus_tag="VHT22_07780"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137493.1"
                     /translation="NCIAEVLGIQEHVAEPLMRTLCRHLSARQVLLVLDTCEHVIAAI
                     AALVAALLAEAPNTHVLATSREALNVDGEQQIPLQPLSLPTAGASLKEIAGAEAVQLF
                     VERARLQRPGFALTQDSAMPVSAICSRLEGIPLAIELAAARLYSLSIEEINQRLDDRF
                     GLLAEGPRASPRRQKTLRATLDWSYDLLAEEEQRALRRLAVFAGGFTLGAARRVVGER
                     TMKDATMLGVLSPLVARSLVIADVTDAGTRYRLLDTMRAYCMEKLDVARERALASRRH
                     ARYFRDRFRYAFEEWLQGSDLHWNAVYLAERDNVHAALDWAFSPQGDAAIGMSITADS
                     GPAWLLWSLRSEGVARVELALTRCTSRTPQRIRARLWLWLGLLDQFTDPVKWIRALRR
                     AVTLHRRAGDALGTGYSLMRLGSALARTGRLDRAQQALDAARPLLARSPVPAVMAPYY
                     HAAGFVRKAVGDLPGARTHYEKGLSLYRSAGLERDAAQLAGALADTNWALGALDEAAT
                     GFREVIEVMRGSNKGTKLVLGVNLTNLAGVLVERGDFEEALGTAREGIELRKTAGYAW
                     GALDHLALRAAQVGRCADAVRIAGYVDAAFAARGIVRQANEARARARLDGVLGDRLDA
                     NEREALMAEGAAMTEEDACRLALAN"
BASE COUNT          353 a          691 c          616 g          331 t
ORIGIN      
        1 ccgtgtaatc ggcgctcctc gctgcatgtc gactgaacgc tcaattcgca agcgccaatc
       61 gacaggcgtc ttcttcggtc attgctgcgc cttctgccat aagcgcttcg cgctcgttgg
      121 cgtccagcct atcgccgagc acgccatcga ggcgcgcgcg ggccctcgcc tcgttcgcct
      181 gccgcacaat ccccctcgct gcgaacgcgg catcgacgta gccggcgatg cgtacggcgt
      241 ctgcgcatct gccgacctgc gcagccctca gcgcgagatg atccagtgca ccccaggcgt
      301 atcctgcggt cttgcgcaac tcgatgccct cccgcgctgt acccagtgcc tcctcgaagt
      361 cgccgcgctc gacgagcacg ccggccaggt tggtgaggtt cacgccaagg accaacttgg
      421 ttcccttgtt cgacccgcgc atcacctcga tgacctcgcg aaatcccgtg gccgcctcat
      481 cgagcgcgcc gagcgcccaa ttcgtgtcgg ccagcgcccc ggccagctgc gccgcgtcgc
      541 gctccaagcc cgcggaccga tagagtgaaa gccctttctc atagtgcgtt cgcgcgccag
      601 ggagatcgcc gacagccttg cgcacgaatc cggccgcatg gtaatacggt gccatgacgg
      661 ccggcacagg gctgcgtgcg agcaatggcc gggccgcgtc gagcgcctgt tgcgcgcgat
      721 cgagccttcc cgtccgcgcc aacgcgctgc ccaggcgcat gagcgagtag cccgtgccga
      781 gtgcgtcgcc ggcccggcga tgaagggtga ctgcacggcg gagcgcgcga atccatttca
      841 caggatcggt gaactggtcc aacagcccga gccagagcca caagcgtgcg cggatccgtt
      901 gcggtgtgcg cgaggtgcag cgcgtcaatg caagctcgac gcgagcgacc ccctcgctgc
      961 gcaacgacca caagagccaa gccggaccgg agtccgctgt gatgctcatg ccgatcgcgg
     1021 catcgccctg gggcgaaaac gcccaatcga gcgctgcgtg cacattgtcg cgctccgcca
     1081 ggtataccgc attccagtga aggtcggaac cctggagcca ttcctcaaac gcgtacctga
     1141 agcgatcccg aaagtagcgc gcatgacgcc gggatgcgag cgcccgctcg cgggcaacat
     1201 cgagcttctc catgcaatac gcacgcatcg tgtcgagcag gcgatagcgg gtgcctgcat
     1261 cggtcacgtc ggctatcaca agcgagcgcg cgacgagcgg ggaaagcacg cccagcatcg
     1321 ttgcgtcctt catcgtccgc tcaccgacga ctcggcgagc tgctccgagc gtgaagccgc
     1381 ccgcaaatac cgccaagcgt cgcagcgcgc gctgctcttc ttcggcgagc agatcgtagc
     1441 tccagtcgag cgtggcgcga agcgtcttct ggcgccgtgg cgatgcgcgc ggcccctcgg
     1501 cgagcaaccc aaagcgatcg tctaggcgct ggttgatctc ttcgatcgat agcgagtaca
     1561 ggcgtgccgc ggccagctcg attgctaacg gtatgccttc gaggcgcgaa caaatcgcac
     1621 tcaccggcat ggcactgtct tgcgtcagcg cgaagccggg ccgctgcaac ctcgcgcgct
     1681 cgacgaagag ctgcacagct tcagcgccgg caatctcctt gaggctggcg ccagcggttg
     1741 gcaacgacaa cggctgcaac ggaatctgct gttcgccatc gacgttcagc gcctcccggc
     1801 tggtcgccag gacatgcgtg ttcggcgcct cagctagcag agcagcaacg agtgccgcga
     1861 tggcggctat cacgtgctcg caagtatcga gcacgagtag gacttgccgc gcactcaggt
     1921 gacgacatag cgttcgcatc aacggctctg cgacgtgttc ctggataccg aggacctccg
     1981 caatgcaatt g
//
LOCUS       DATANS010000531         6480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_152743, whole genome shotgun sequence.
ACCESSION   DATANS010000531 DATANS010000000
VERSION     DATANS010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6480
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152743"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VHT22_07785"
     CDS             <1..737
                     /locus_tag="VHT22_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008057078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX3137494.1"
                     /translation="VLLMPDQMMLQIHESIGHPLELDRILGDERNFAGTSFVTLDMFG
                     TYRYGSDLLNVSFDPTRPEELASYGFDDDGAPAEKAYLIRRGILERPLGGTISQARAK
                     TLGVANSRADGWNRPPIDRMANLNIEPGDADLEAMIAGIEHGVLMATNSSWSIDDSRN
                     KFQFGCEWGQLIDRGRLTTVVKNPNYRGISANFWRSLTRVGDAATVEVLGTPYCGKGE
                     PSQLIRVGHASPACVFTGVDVFGGDN"
     gene            737..2074
                     /locus_tag="VHT22_07790"
     CDS             737..2074
                     /locus_tag="VHT22_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX3137495.1"
                     /translation="MAISTMETYFNELAVFLDRALLPGEVYTCTFDAEVSDFVRMNRG
                     KIRQPGTVEQRYVRLHLIVGRKHAQHCMTVCGDLARDREPLLQALAALRDMVTDLPDD
                     PHLHYATEVHSSRVVRSNPLPAPEGVVATVLDSAQGLDLVGIYAAGPVYRGFANSLGQ
                     RNWHEVTSFNLQWSLYHRADKAVKTAYGGFDWSDEAFAAKMRGARERLALLAVPAKSL
                     EPGSYRAYLTPTAMEEIGAMLCWGGFSGRALATRQSCLFRMQEGAAALDASVSFSENT
                     ASGVAPAFQDEGFARPASVPLVTNGRLVGSLVSPRTAKEFELATNGANASESPESLDM
                     AGGQLGGDDALAALDTGLYIGNLWYLNFSDRPACRVTGMTRFASFWVEHGRIVAPVNV
                     MRFDDSVYRLFGEHLVGLTSESELVVSDQSYRARSVSSMRLPGAVVSEMAFTL"
     gene            complement(2361..4052)
                     /locus_tag="VHT22_07795"
     CDS             complement(2361..4052)
                     /locus_tag="VHT22_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005041039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3137496.1"
                     /translation="MTAPLLSIRNLRVNFRLDKDTTFEAVKGISFDIPMNSTVALVGE
                     SGSGKSVTSLSILGLLPKENATVLPGSEIIYGGRNLVELGQDELRRIRGADISMIFQE
                     PMTSLNPVFTVGFQLTEVLRMHMGMRPAEARKRAIALLAEVGIPDPKFKINAYPSQMS
                     GGQQQRVMIAMAIACEPKLLIADEPTTALDVTIQKQILDLIAALQKKHQMSVLFITHD
                     LAVVGEIADYVVVMRNGEIREQGPAKAVFENPQDAYTKALLLCRPRLDRRPARLPVIE
                     DFMNGHGQAMHLTERKRGIDRVDPIILDVRHLGKDFYSREGLFGRRKFEAVKDVSFRL
                     PKGKTLGLVGESGSGKTTVGLTLMRLHEATTGEALFEGKDILSMSPRQFMDYKRRIQI
                     IFQNPYASLNPRMIVGQILTEPMQIHGIGKSVQERTELAFELLQKVGLPEVSFYKYPH
                     EFSGGQRQRIAIARCLTLKPDILICDESVSALDVSVQAQVLNLLQDLQDEFRLSYIFI
                     SHDLAVVKYISDEIMVMNNGAIVEIANSDDIYLHPQHQYTKKLLSSIPKGWHDAS"
     gene            complement(4099..5241)
                     /locus_tag="VHT22_07800"
     CDS             complement(4099..5241)
                     /locus_tag="VHT22_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005251093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3137497.1"
                     /translation="MPYDAPHAVSPGLWALAWRRLRSDRVAMISLAIVAAFMLMIVLS
                     ATGLIARDWAREEGVNYAPPTFVGPDAATAPAGAVPGAPVAGEPGTAENESDLQIADP
                     LADALAAIKAGATKPGEASPPAPDTKSVDPLADVMADIKAKGAGRADAVATRAATLPF
                     GGDKWGRDVLKKVIKGSQTSIFVGIAAAIVAVFLGSVFGAVAGYYGKWLDDVFNWFYS
                     VFTAIPYLLLILAVAAVLQQKGTLTIILILGLTGWTGVFRLIRGEYLKHKGREYVQAA
                     DAIGASNARRMFVHILPNVSHVVLVQLSILTVAFIKSEVILSFLGFGVPVDVVSWGSM
                     LNEAQNELILGKWWQLAAAGTFMALLVTAFSLFTDALRDALDPKLK"
     gene            complement(5252..6190)
                     /locus_tag="VHT22_07805"
     CDS             complement(5252..6190)
                     /locus_tag="VHT22_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3137498.1"
                     /translation="MTAYIIRRVWQMIPTLLGVILLVFILFNWVGGDPAYVLAGKISS
                     AEQIENIRRQLGVDQPFYVQLWIFIKQVLTADFGASWSTNEAVSHIMATRLGPSLTVL
                     IPLLIISTLVAIGLALAVAYVRGSLTDRVVMIVCTVALSVSILVYIIVFQYFFAYKLG
                     WFPVQGWGDGFWHNLIVYASLPIIILLVVSIAPDLRLFRTFVLDEINQDYVRTARAKG
                     VSENRIMWVHVLRNASIPIITHVMAQLPGLLIGAFLVERFFSIPGIGREVILAVERSD
                     FPVIKAITVYVAVATMVVNLLTDLMYKAVDPRVQLK"
     gene            complement(6240..>6480)
                     /locus_tag="VHT22_07810"
     CDS             complement(6240..>6480)
                     /locus_tag="VHT22_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX3137499.1"
                     /translation="TAGYYGYMWSQVLALDMLSKFDGNLMDPATGKRFRTTVLAQGSQ
                     KPAAQLVREFLGREPSNEAFIAEITGTRSTAMLAH"
BASE COUNT         1152 a         2083 c         2115 g         1130 t
ORIGIN      
        1 acgtgctgct gatgccggac cagatgatgc tgcagatcca cgagagcatc ggccacccgc
       61 tggagctgga ccgcatcctg ggcgacgagc gcaacttcgc cggcaccagc ttcgtcacgc
      121 tggacatgtt cggcacctac cggtacggat ccgatctgct caacgtcagc ttcgatccga
      181 cgcgaccgga agagctggcg agctacggct tcgacgacga cggcgcgccc gccgagaaag
      241 cgtacctgat ccgccgcggg attctcgagc ggccgctggg cggcacgatc tcgcaggcgc
      301 gcgccaaaac gctgggcgtg gccaactcgc gggccgatgg ctggaaccgg ccgccgatcg
      361 accgcatggc caacctcaat atcgagccgg gcgatgccga cctcgaggcg atgatcgctg
      421 gcatcgagca cggcgtcctg atggcgacca acagctcctg gtcgatcgac gactcgcgca
      481 acaagttcca gttcggctgc gagtggggac agctgatcga tcgcggccgc ctcaccaccg
      541 tcgtcaagaa cccgaactac cgcggaattt cggccaactt ctggcgcagc ctcacccggg
      601 tcggcgatgc cgcgacggtc gaggtgctcg gcacccctta ttgcggcaaa ggcgagccgt
      661 cacagctgat ccgcgtcgga catgcctcgc ccgcctgcgt gttcaccggc gtcgacgtct
      721 tcggggggga caactgatgg cgatctcgac gatggagacc tacttcaacg aactggccgt
      781 ctttctcgac cgcgcgcttc tcccgggaga ggtttacacc tgcacattcg atgccgaggt
      841 atcggatttc gtgcgcatga accgcggcaa gatccgccag ccgggaacgg tggagcagcg
      901 ttatgtccgg ctgcacctga tcgtcggtcg caagcatgcc cagcattgca tgaccgtttg
      961 cggggacctg gcgcgcgatc gcgagccgct gctgcaggcg ctggccgcgt tgcgagacat
     1021 ggtgacggat ttgcccgacg atccgcattt gcattatgcg accgaggtcc attccagccg
     1081 ggtcgtccgg agcaatccgc tgcccgcgcc cgaaggcgtc gtcgcgaccg tcctcgactc
     1141 ggcgcaaggg ctcgacctgg tcggcatcta cgccgcgggg ccggtctatc gcggctttgc
     1201 caactcgctc gggcagcgca attggcatga agtgacgagc ttcaatctgc agtggagtct
     1261 ctatcaccgc gcagacaagg cggtgaaaac ggcttacggc ggcttcgact ggtcggatga
     1321 ggcgttcgcc gcgaaaatgc gcggtgcccg cgaacgtctg gcgctgcttg ccgttccggc
     1381 aaaatcgctc gagcccggaa gctatcgcgc gtatctgacg ccgacggcga tggaggagat
     1441 cggtgccatg ctctgctggg gcggattctc cggacgcgcg ctggcgacgc ggcaaagctg
     1501 cctgttccgg atgcaggaag gcgcggcggc gctcgacgct tcggtgtcgt tcagcgaaaa
     1561 caccgccagc ggcgtcgcgc ccgcgtttca ggacgagggg tttgcgcgcc ccgcctcggt
     1621 gcccctggtc acgaacggaa ggctcgtcgg ctcgctggtc agcccgcgga ccgcgaagga
     1681 attcgagctt gctaccaacg gcgccaatgc atcggagagc ccggaatcgc tcgatatggc
     1741 gggcggccag cttggcggcg acgacgcgtt ggcggcactc gacaccgggc tttacatcgg
     1801 caatctctgg tacctgaact tttccgaccg ccccgcctgc cgcgtgaccg ggatgacgcg
     1861 cttcgcctcg ttctgggtcg agcatgggcg gatcgtcgcg ccggtcaacg tcatgcgctt
     1921 cgacgacagc gtgtaccggc tgttcggcga gcacctggtc ggattgacgt cggagagcga
     1981 gctcgtcgtc agcgaccaga gctaccgcgc gcgcagcgtc agcagcatgc gtctgccggg
     2041 cgcggtggtc agcgagatgg cgtttacctt gtaggacgcc gcagggcgga gcaagcaggg
     2101 gccccgcgcg tagcgcgggg atgcgacccc ggagacggaa ggccggcgaa aacgagggtc
     2161 gcactcgtcg cggtgctgtt gcggaagccg gcgacgccgc agggcggagc agggatgcga
     2221 ccccggaggc ggaaggccgg cgaaaacgag ggtcgcactc gtcgcggtgc ttttgcggaa
     2281 gccggcgacg ccgcagggcg gagttccccg cacgtagcgc ggggatgcga ccccggaggc
     2341 gcgagctggc cggcgaagcc ctacgaggcg tcgtgccagc ccttcggtat cgacgacaac
     2401 agcttcttcg tgtactggtg ctgcggatgc aggtagatgt cgtcggagtt ggcgatctcc
     2461 acgatcgccc cgttgttcat caccatgatc tcgtcggata tgtatttgac gacggcgagg
     2521 tcgtgtgaaa tgaagatgta ggacagccgg aattcgtcct gcaggtcctg cagcagattc
     2581 agcacctgcg cctgcactga cacgtcgagc gccgataccg attcgtcgca gatgagtatg
     2641 tccggcttga gcgtcaggca gcgcgcgatc gcgatgcgct gccgctgccc gccggagaac
     2701 tcgtgcgggt atttgtagaa cgagacttcg ggcaggccga ccttttgcag cagctcgaag
     2761 gccagctcgg tgcgttcctg caccgacttg ccgatgccgt ggatctgcat cggctcggtc
     2821 aggatctggc cgacgatcat ccgcggattg agcgacgcgt acgggttctg gaagatgatc
     2881 tggatccggc gcttgtagtc catgaactgg cgcggcgaca tcgacaggat gtccttgccc
     2941 tcgaacagcg cctcgcctgt cgtcgcctcg tgcagacgca tcagcgtcag tcccaccgtc
     3001 gtcttgcccg atccggactc gccgaccagc ccgagcgtct tgcctttcgg caaccggaat
     3061 gaaacgtcct tgaccgcctc gaacttgcgc cttccgaaca ggccctcgcg cgaatagaag
     3121 tccttgccga ggtggcgcac gtccaggatg atcggatcga cgcgatcgat gccgcgcttg
     3181 cgctcggtca ggtgcatcgc ctggccgtgg ccgttcatga aatcctcgat caccggcagg
     3241 cgggcgggcc ggcggtccag gcgcggccgg catagcagca gcgccttcgt atatgcgtcc
     3301 tgcggatttt cgaacacggc cttcgccggc ccctgctcgc ggatttcgcc gttgcgcatc
     3361 accacgacgt agtctgcgat ctcgccgacg acggccagat cgtgcgtgat gaacagcacc
     3421 gacatctggt gctttttctg cagcgccgcg atcagatcga ggatctgctt ctggatcgtc
     3481 acgtccagcg ccgtcgtcgg ctcgtcggcg atcagaagct tgggctcgca ggcgatggcc
     3541 atcgcgatca tcacccgctg ctgctggcca cccgacatct gcgacggata cgcattgatc
     3601 ttgaatttcg ggtcgggaat gccgacctcg gccagcaacg cgatcgcgcg ctttctcgcc
     3661 tccgccggtc gcatgcccat gtgcatgcgc agcacctcgg tcagctggaa cccgacggtg
     3721 aacaccgggt tcagcgatgt catcggctcc tggaagatca tcgagatgtc ggcgccccgg
     3781 atcctgcgca attcatcctg gccgagctcg acgagattcc gtccgccgta aatgatctcg
     3841 ctgcccggca gcaccgtcgc gttctccttc ggcagcaagc cgagaatcga cagcgacgtc
     3901 accgacttgc cgctgcccga ttcgccgacc agcgcgaccg tcgagttcat cggaatgtcg
     3961 aagctgatgc ctttgacggc ttcgaaggtc gtgtctttgt cgagtcggaa attgacccgg
     4021 aggttacgga tcgaaagcag cggcgcggtc atcgtgtttt tccggtcgca aattgcacgg
     4081 gcagcggggc gatcttggtc atttcagttt cgggtccagt gcgtcgcgca aagcgtcggt
     4141 gaacaggctg aacgcggtca ccagcagcgc catgaaggtc ccggctgccg cgagctgcca
     4201 ccattttccg aggatcagct cgttctgcgc ctcgttcagc atcgagcccc acgagacgac
     4261 gtcgaccggg acgccaaagc cgaggaacga aaggatcacc tcggacttga tgaaggcgac
     4321 cgtcagtatc gaaagctgga ccagcacgac atggctgacg ttgggcagga tgtgcacgaa
     4381 catgcggcgg gcgttcgacg caccgatcgc gtcggcggcc tgcacgtact cgcgcccctt
     4441 gtgcttcaga tattcgccgc ggatcagacg gaacaccccg gtccagccag tcaggccgag
     4501 gatgaggata atggtcagcg tgcctttttg ctgcagcacc gcggcgacgg ccaggatcag
     4561 cagcaggtag ggaatcgccg tgaacacgct gtagaaccag ttgaaaacgt cgtcgagcca
     4621 cttgccgtaa tagccggcga ccgcgccgaa caccgagccc aggaacaccg ccacgatcgc
     4681 ggcggcgatg ccgacgaaga tcgaggtctg cgagcccttg atcacttttt tcagcacgtc
     4741 gcggccccac ttgtcgccgc caaaaggcaa ggtcgccgcg cgggtggcga ctgcatcggc
     4801 gcgccctgcg cctttcgcct tgatgtcggc catcacgtcg gcgagcgggt cgacgctctt
     4861 ggtgtcgggc gccgggggcg acgcctcgcc cggcttggtc gctccggcct tgatcgcggc
     4921 cagcgcgtcg gccagcgggt ccgcgatctg caggtcggat tcgttttcgg ccgtcccggg
     4981 ctccccagcc accggtgcgc ctggcactgc tccggccggc gcagtcgccg cgtcgggtcc
     5041 gacgaacgtg ggcggcgcgt agttgacgcc ctcctcccgg gcccagtccc tggcgatcag
     5101 cccggtggcc gacaggacga tcatgagcat gaaggcggcg acgatcgcca gcgaaatcat
     5161 cgccacgcgg tccgagcgca gccggcgcca ggccagcgcc cagaggccgg gagagacggc
     5221 gtgcggagca tcgtagggca tcgtccgtct cctatttgag ttgcacgcgc ggatcgaccg
     5281 ccttgtacat caggtcggtc aacagattga cgaccatcgt cgccaccgcg acatagacgg
     5341 tgatcgcctt gatcacagga aagtcgctgc gctcgaccgc gagaatcacc tcgcgaccga
     5401 tgcctgggat cgagaaaaag cgttcgacca ggaacgcgcc tatcagcaag ccgggcagct
     5461 gcgccatcac gtgcgtgatg atgggaatcg acgcattgcg cagaacgtgc acccacatga
     5521 tgcgattttc cgacacgccc ttggcacgcg cggtgcggac gtagtcctgg ttgatctcgt
     5581 cgagcacgaa cgtgcggaac agccgcaggt cgggcgcgat gctgacgacc agcaggatga
     5641 tgatcggcag cgacgcgtag acgatcaggt tgtgccagaa gccgtcgccc cagccttgca
     5701 ccgggaacca gccgagcttg taggcgaaga agtactggaa cacgatgatg tagaccagga
     5761 tcgagaccga cagcgccacc gtgcacacga tcatcacgac gcgatcggtc agcgagccgc
     5821 gcacgtaggc gaccgcgagt gcgaggccga tcgcgaccag cgtgctgatg atcagcagcg
     5881 gaatcagcac cgtcagcgat gggccgaggc gcgtcgccat gatgtgcgac accgcctcgt
     5941 tggtgctcca gctggcgccg aaatcagcgg tcaggacctg cttgatgaat atccacagct
     6001 gcacgtagaa cggctgatcg acgccgagct gacgccggat gttctcgatc tgctcggcgc
     6061 tggaaatctt gccggccagc acataggcgg gatcgccgcc aacccagttg aacaggatga
     6121 acaccagcag gatgaccccg agcagggtcg ggatcatctg ccatacccgc cggatgatgt
     6181 acgccgtcat gtttcctcct gctcgcgcct gaagcaccgc cccggcgctt tgcgccgctt
     6241 tagtgtgcca gcattgccgt cgaccgtgtg ccggtgattt cggcgatgaa cgcctcgttc
     6301 gacggctcgc gaccgagaaa ctcccgaacc agctgcgccg cgggcttctg gctgccctgg
     6361 gcgaggaccg tcgttcggaa ccgcttgccg gtcgccggat ccatgaggtt gccgtcgaat
     6421 ttcgacaaca tgtcgagtgc cagcacctgc gaccacatgt atccgtaata gccggccgtg
//
LOCUS       DATANS010000532         2892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_489702, whole genome shotgun sequence.
ACCESSION   DATANS010000532 DATANS010000000
VERSION     DATANS010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489702"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VHT22_07815"
     CDS             complement(<1..504)
                     /locus_tag="VHT22_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004682881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3137500.1"
                     /translation="MLGIAQRGLISLLAAAIALAPTAAPAALAADPAKVLRIEFYVAE
                     TGFDPVKVQDYYSQTVCEAIFESLMTYDYLARPAKLAPRAAEAMPVISDQARTYVFKI
                     RKGIYFAPDPVFKGKPRELTAEDYAYTIKRFRDPANKSPYESFLGGVLGLDEVKKDAE
                     KTGKFDYD"
     gene            complement(563..2365)
                     /locus_tag="VHT22_07820"
     CDS             complement(563..2365)
                     /locus_tag="VHT22_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3137501.1"
                     /translation="MGRPFLRFVIVGGCLLTLSGVPAQIASAAADPNKVLHVAIEAGD
                     DGFDPSRSTNYYSGLIEEVIFERLLTYDYLAEPVKLAPMLAEALPEVADGGKTYTFHL
                     RRGVYFTPDPAFKSDKRELVAEDVAYSIKRFLDPKNRSPWRFLFDGKIVGLNEQEALA
                     KKNGDRFDYDARIPGLEVVDRYTIRFHLIDVDFNFAYILASPTNSIVAREVIERYGDD
                     TKGHPVGSGPYMLAEWQRGARILLHANPGYRGFVWDFQPSGNPWDQDVVAAMKGKQMP
                     QIGVVDVRLMEEEQSRWLAFQGAEIDYIDRFGSFAPMAIPNNKVAPDLAKRGIKLYRY
                     LEPEMTYYYFNMVDPVVGGFDKEKIALRRAIAMSYDIDEEIRIIRKNQAIRAESPIPP
                     GVVGYSPNYRSGYHYDPALANKLLDYFGYKRGDDGYRTLPDGKPLLITLWSTPEAIAR
                     EYDELWKKSLDVIGVHFATSKIKFADQIQKGRACQMQFAGAAWTADWPTADNFLQLSY
                     GPNVGESNYACYKSAAFDEFYRQAQRLPDSPERNRLYRAMARQMEVDTTWVMGATRYK
                     NTLFYPWMLGYKKHPILHAPWPFMDIDNSRRPAK"
     gene            2509..>2892
                     /locus_tag="VHT22_07825"
     CDS             2509..>2892
                     /locus_tag="VHT22_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX3137502.1"
                     /translation="MKPPFWDEAARELSRRDRTLRRLIRAYPGIHLKRRADPFTTLAR
                     AIVGQQISVKAADAIWWRFVDVADNAPAHKFRRLPAAKVAAADMAALRGCGLSQRKAE
                     YLSDLARHFASGKLDPKRWRKLDDES"
BASE COUNT          512 a          923 c          909 g          548 t
ORIGIN      
        1 gtcgtagtcg aacttgcctg tcttttcggc gtccttcttg acttcgtcga gcccgagcac
       61 gccgccgaga aaactctcgt acggcgactt gttggccgga tcgcggaagc gcttgatcgt
      121 gtaggcgtaa tcttcggccg tcaattcacg cggcttgccc ttgaacaccg gatccggcgc
      181 gaagtagatg cccttgcgga tcttgaacac gtacgtccgc gcttgatcgc tgatcacggg
      241 catcgcttcg gcagcacgcg gcgcgagctt cgcgggccgc gccaggtagt cgtaagtcat
      301 cagcgattcg aagatcgctt cgcagaccgt ttgcgaataa tagtcctgca ccttgaccgg
      361 gtcgaagccg gtctcggcga cgtagaactc gatgcgcagg accttggccg gatcggccgc
      421 gagcgcggcc ggagccgcgg taggcgcaag cgcgatcgcc gcggcgagca gcgatatcag
      481 gccgcgctgc gctattccga gcatcggtgc agcatcgccg cgagcatcgg tgtcagaccg
      541 aacaagcgac acgggactcg cactatttgg ccggacgcct ggaattgtcg atgtccatga
      601 acggccaggg cgcgtgcagg atcggatgct tcttgtaacc cagcatccag gggtagaaaa
      661 gcgtgttctt gtagcgcgtc gcgcccatca cccaggtcgt gtcgacttcc atctgccgcg
      721 ccatcgcccg gtagaggcga ttccgctccg gcgaatccgg cagcctctgc gcctgccgat
      781 agaattcgtc gaatgccgcc gacttgtagc aggcatagtt ggattctccg acgttcggac
      841 cgtaggacag ctgcaggaag ttgtccgcgg tcggccagtc cgccgtccaa gccgcaccgg
      901 cgaattgcat ctggcaggcc ctgccttttt gtatctgatc ggcaaacttg atcttcgacg
      961 tcgcgaaatg gacgccgatc acgtcgaggc tttttttcca gagctcgtcg tactcgcggg
     1021 cgatcgcttc gggcgtcgac cacagcgtga tcagcagcgg cttgccgtcc ggcagcgtgc
     1081 ggtatccgtc gtcgccgcgc ttgtatccga aataatcgag cagcttgttc gccagcgcgg
     1141 gatcgtagtg atagcccgag cggtagttcg gactgtagcc gacgacaccg ggcggtatcg
     1201 gcgactcggc tcggatcgcc tggttcttgc ggatgatgcg tatttcctcg tcgatgtcgt
     1261 acgacatcgc gatcgcgcgc cgcagcgcaa tcttctcctt gtcgaatccg ccgacgaccg
     1321 gatcgaccat gttgaagtag taataggtca tctccggctc gagatagcgg tagagcttga
     1381 tgccgcgctt ggccagatcc ggtgcaacct tgttgttcgg aatggccatc ggcgcgaacg
     1441 agccgaagcg atcgatgtag tcgatctccg cgccctggaa cgccagccag cgcgactgct
     1501 cctcctccat caggcgcacg tcgacgacgc cgatctgcgg catctgcttg cccttcatcg
     1561 ccgcgacgac gtcctggtcc cacggattgc ccgacggctg gaagtcccag acgaagccgc
     1621 gataccctgg gttggcgtgc agcagaatgc gggcgccccg ctgccactcc gccagcatgt
     1681 acggcccgga gccgaccggg tgccccttgg tatcgtcgcc ataacgctcg atgacttcgc
     1741 gcgcgacgat gctgttcgtc ggcgaggcca atatgtaggc gaagttgaag tcgacgtcga
     1801 tcagatgaaa gcggatcgta tagcgatcga cgacttcgag tccgggaatc cgggcgtcgt
     1861 agtcgaaccg gtcgccgttc tttttcgcca gcgcctcctg ctcgttcagg ccgacgatct
     1921 tgccatcgaa cagaaatcgc cagggcgagc gattcttcgg atcgaggaag cgcttgatcg
     1981 aataggcgac atcttccgcc accagctcgc gcttgtcgct cttgaacgca gggtcgggcg
     2041 taaaatacac gcccctgcgc agatgaaacg tataggtctt gccgccatcg gcgacttcgg
     2101 gcagggcttc ggccagcatc ggcgcgagct tgaccggctc agccaggtag tcgtaggtga
     2161 gcagtcgctc gaaaatgact tcttcgatca gtccggaata gtaattggtg cttcgcgacg
     2221 gatcgaagcc gtcgtcgccg gcttcgatcg caacgtgcag caccttgttc ggatcggcgg
     2281 cggctgacgc gatctgcgcg ggaacgccgg acagcgtcag caggcaacct ccgacgatga
     2341 cgaagcgcag aaacggacgg cccatcgatc cctctccggc gaaaggcgcg taatgtacca
     2401 cttccagccc cggctcgacg cccgttgcga cggagcgcgg cgtgcgggcg cgagcttggc
     2461 gcccgcggcg atcggacgcc tgcccgcgcc gcgcacgccg ccggcccgat gaagccgccg
     2521 ttctgggacg aggccgcgcg cgaactctcg cgccgcgacc gcaccctccg ccggctgatc
     2581 cgcgcttacc ccggcatcca tctgaagcgc cgcgccgatc cattcacgac gctggccagg
     2641 gcaatcgtcg gccagcagat atcggtcaag gcagccgacg ctatctggtg gcgttttgtc
     2701 gatgtggcgg acaatgcgcc ggcacacaag ttccggcgtc ttcccgcggc aaaagtcgcc
     2761 gcggccgaca tggcggcatt gcgcggctgc ggattgtcgc aacgcaaggc ggagtacctg
     2821 tcggacctcg cgcggcattt cgcctccggc aagctcgatc cgaaacgctg gcgcaagctc
     2881 gacgacgagt cg
//
LOCUS       DATANS010000533         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_530419, whole genome shotgun sequence.
ACCESSION   DATANS010000533 DATANS010000000
VERSION     DATANS010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530419"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1611
                     /locus_tag="VHT22_07830"
     CDS             <1..>1611
                     /locus_tag="VHT22_07830"
                     /inference="COORDINATES: protein motif:HMM:NF044699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEX3137503.1"
                     /translation="SFIHIHLASVIDDAARDQLMRGLEGVLGEVRVAVQDWKTMRERV
                     DAIVTELKNNPPPLPVGEVAEAIQFLQWLLADNFTFLGLRDYRVDGQALEPDFESTLG
                     IMRSQELRVLKRGNELLEFTPEIMAFLKEPRPLIVAKANIHSRVHRRAYLDYIGVKRF
                     DSAGNLIGEHRIIGLFTSTAYTRTAHSIPYLRRKIAAVEARAGFDPGSHSGKALANVL
                     EHYPRDELFQIDEDTLYSFATAILQLDERPRVRVLARHDRFDRFVSVLVFVPRERYDS
                     DIRAKIGVYLARAFIGRVSAFDPYFPGEGSLVRVHFIIGRSGSGASPDIDRATLEREV
                     SAIIRTWTDGLSRALELVNPPEKAREIFNLYRDAFSEGFHEAYAPTVAAGDIRVVEGL
                     SEQRPLGVDFHHRLEEEQRSVGLKVWSFARPLPLSERVPVLENMGFRVVDERTYHIAP
                     QGEAGAWLHDMLLERADGGMIDLDSTKGRLEAAFLMVMRGIAENDGYNALTLQGGLAW
                     RDVALVRAISRFLRQIRVPYSQDYMWATL"
BASE COUNT          282 a          503 c          515 g          311 t
ORIGIN      
        1 agtttcattc atattcatct ggcttcggtc attgatgatg ccgcgcgcga ccagctcatg
       61 cgcgggctcg agggtgtgct cggggaagtg cgcgtcgcgg tgcaggactg gaagacgatg
      121 cgcgaacgcg tggacgccat cgtcaccgaa ctgaagaaca atccgccgcc gctgccggtc
      181 ggtgaggtcg ctgaagccat tcaattcctg caatggctcc tcgccgacaa tttcactttc
      241 ctcggcctgc gcgactatcg cgtggacggg caggcgctcg aaccggattt cgagagcacg
      301 ctcggcatca tgcgctcgca ggaattgcgc gtgctcaagc gcggcaacga gcttctcgag
      361 ttcacgccgg agatcatggc gttcctgaaa gagccgcggc cgctgatcgt cgccaaggcg
      421 aacatccatt cgcgcgtgca ccggcgcgcg tatctcgact atatcggcgt caaacgtttc
      481 gattccgccg gcaatctgat cggcgagcac cggatcatcg ggctttttac atcgacggcg
      541 tatacgcgca ccgcgcacag cattccgtac ctgcggcgga agatcgcggc cgtcgaggcg
      601 cgcgccggct tcgatccggg cagccattcc ggcaaggcgc tggcgaatgt gctcgagcac
      661 tatccgcgcg atgaactttt ccagatcgat gaggacacgc tctacagttt cgcaacggcg
      721 atcctgcagc tcgacgagcg tccgcgcgtg cgcgtgctcg cgcgacatga tcgcttcgat
      781 cgtttcgtct cggtgctggt gttcgtgccg cgcgaacgtt acgacagcga catccgcgca
      841 aagatcggcg tctatctggc gcgcgccttc atcggccgcg tctcggcgtt cgatccgtat
      901 ttccccggcg aaggatcgct ggtgcgcgtg cactttatta tcggccggtc cggcagcggt
      961 gcctcgccgg acatcgatcg cgcaacgctc gaacgcgaag tctcagcgat catccgcacc
     1021 tggaccgatg gactgagccg cgcgctcgaa ctggtcaatc cgccggagaa ggcgcgcgag
     1081 attttcaatc tttaccggga cgcgttctcg gaaggattcc acgaggccta cgcgccgacc
     1141 gttgcagccg gcgacattcg cgtggtcgaa gggctttcgg aacagagacc gctcggcgtt
     1201 gatttccacc atcggctcga ggaagagcaa cgctcggtcg gcctgaaagt gtggagtttc
     1261 gcgcggcctt tgccgctgtc agaacgcgtg cccgtgctgg aaaacatggg ctttcgcgtg
     1321 gtcgacgagc gcacctatca catcgcgccg cagggcgagg caggcgcctg gctgcacgac
     1381 atgctgctcg agcgtgccga cggcggcatg atcgatctcg acagcaccaa gggacggctc
     1441 gaagcggcgt tcctcatggt gatgcgcggc atcgccgaga acgacggcta caacgcgctg
     1501 acgctgcagg gcggccttgc ctggcgcgat gtggcgctgg tgagggcgat atcgcgcttc
     1561 ctgcgccaga ttcgcgtgcc ttattcgcaa gattacatgt gggccacgct g
//
LOCUS       DATANS010000534         1637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_612106, whole genome shotgun sequence.
ACCESSION   DATANS010000534 DATANS010000000
VERSION     DATANS010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612106"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VHT22_07835"
     CDS             complement(<1..707)
                     /locus_tag="VHT22_07835"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX3137504.1"
                     /translation="MRSDSQPGRSDSVPKIGDVLDDVDTPALIVELDALERNVAAMAA
                     AVEDRGVRLRPHAKSHKSPDIARRQLAAGAVGICCQKVGEAEAFVAAGVTDVLITNEI
                     VGAAKLARLALLSRNARLGVLVDDRVNVGSLAAAMRAAGASIDVLVEVDVGAHRCGVL
                     PGAPALELARAIAGESGLRFAGLHAYQGAAQHLRRPAERRAAIAQAAELASETKALIE
                     QAGIECTTVTGAGTGTFT"
     gene            complement(710..1498)
                     /locus_tag="VHT22_07840"
     CDS             complement(710..1498)
                     /locus_tag="VHT22_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018142018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3137505.1"
                     /translation="MRAAIVIPAFNEARTIRDIARRSLQQCDTVIVVDDGSTDGTGDA
                     IKGLPVTLLRHEVNRGKAAALWTGFTSALAGGTDVVVTLDGDGQHRPEDIPLLIAAAH
                     IYPHRLIVAARLRGRDKYPRARYFANRLADFCVSWAAGHAVADSQSGQRLYPAALLEG
                     ISLRHDEKAAFTLESELIIEAARRGYTCVGVPILAIYDPAGRRSHFRPVRDIARIGWM
                     IALRLLGTGMNPSGLWRCLREPPCIADLAEGVGAERVEPVAESA"
     gene            complement(1498..>1637)
                     /locus_tag="VHT22_07845"
     CDS             complement(1498..>1637)
                     /locus_tag="VHT22_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3137506.1"
                     /translation="KLIKELVPMQRAGTPEDVAQLVGFLVSDNAAYISGQVLSVNGAM
                     Y"
BASE COUNT          281 a          561 c          534 g          261 t
ORIGIN      
        1 gtgaaggtcc cggtgccggc gccggtcacc gtggtgcatt cgattccggc ctgctcgatc
       61 agtgccttgg tctcggaggc cagctcggcc gcctgcgcga tcgccgcgcg gcgctcggcc
      121 gggcgccgca agtgttgcgc cgctccctga taagcgtgca ggccggcaaa gcgaaggccg
      181 gactcgccgg caatggcgcg cgccagctcg agcgcgggcg cgccgggcag cacgccgcag
      241 cgatgggcgc cgacgtcgac ttcgaccagc acatcgatcg acgcgcccgc cgcccgcatc
      301 gccgcggcca gcgaaccgac attgacgcgg tcgtcgacca gcacgccgag ccttgcgttg
      361 cgtgagagca acgcgagacg cgcgagcttc gcggcaccga cgatttcatt ggtgatcagc
      421 acgtcggtta cgccggcagc gacaaacgcc tcggcctcgc cgaccttctg gcaacagatg
      481 ccgaccgcgc ccgcggcgag ctgacgcctg gcaatgtccg gagacttatg gctcttggcg
      541 tgcggtcgca ggcgcacgcc gcggtcttcg actgccgcag ccatcgccgc gacgttgcgt
      601 tccagcgcgt cgagttcgac gatcagcgcg ggagtgtcga cgtcgtcgag tacgtcgccg
      661 atcttgggaa cagaatcgga gcggccaggt tgcgaatcgc tccgcattgt caggcgcttt
      721 ccgcaacggg ctccacgcgc tcggccccga ccccctccgc aagatcggcg atgcacggcg
      781 gctcgcgcaa gcaacgccac aggccggaag gattcatccc ggttccaagc aaccggagcg
      841 cgatcatcca cccgatgcgc gcgatatcgc ggaccggccg aaagtggctg cggcgccccg
      901 ccgggtcgta aatggccaga atcggcacgc ccacgcaggt ataaccgcga cgcgcggcct
      961 cgatgatgag ttcgctttcg agcgtgaatg cggccttttc gtcatgccgc agcgagatgc
     1021 cttcgagcag cgcagccgga tagaggcgtt gccccgactg cgaatcggcc actgcgtgcc
     1081 ccgcggccca cgacacgcag aagtccgcga gccgatttgc aaaatagcgg gcgcgcggat
     1141 acttgtcccg gccgcgcaga cgcgccgcca cgatcagccg atgcggatag atgtgcgccg
     1201 ccgctatcag cagtggtata tcctcggggc gatgctgtcc gtcgccgtcc agcgtgacga
     1261 cgacatcggt accgccggcg agcgcggaag taaaaccggt ccacaacgcc gctgccttgc
     1321 cgcggttgac ctcatgtcgc agcaaggtca ccggcagtcc tttgatcgcg tcgccggttc
     1381 cgtcggtcga gccatcgtcg acgacgatga ccgtgtcgca ctgttgcagc gaacgccggg
     1441 caatgtcccg tatcgtccga gcttcgttga aagcggggat gacaatggct gcgcgcatca
     1501 gtacatcgcc ccgttgaccg acaaaacctg tcccgaaata taggctgcgt tgtcggatac
     1561 gagaaacccg accagctgcg ccacgtcctc gggcgttcct gcgcgttgca tcggaacgag
     1621 ctccttgatt agcttgg
//
LOCUS       DATANS010000535         3057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_405565, whole genome shotgun sequence.
ACCESSION   DATANS010000535 DATANS010000000
VERSION     DATANS010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405565"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VHT22_07850"
     CDS             <1..589
                     /locus_tag="VHT22_07850"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3137507.1"
                     /translation="AIAEEFPTTKIIALEVDAIQHARNLAITDLPNVRFVAGGAEALP
                     LDDDSVDLVLMFKSLHHVPNHLLDAALEEVRRVLVPGGLAYISEPVFAGEYNEIMRIF
                     HDEERVRGAAFSAITRSVERGALEFVTERFFNTPLRFDDFAHFEERVLGVTHTQHRLS
                     ESQLSAVRDRFARHVTPQGVKFLQPMRVDLLRKPA"
     gene            676..2109
                     /locus_tag="VHT22_07855"
     CDS             676..2109
                     /locus_tag="VHT22_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX3137508.1"
                     /translation="MQHEKVRTQDEPATVVRELVRRARAAQRVADGWSQERTDEVVMA
                     AGWAIVEPARNRALAEIAVRDTNLGNVADKIAKNRRKTMGLLRDLQHARSVGVIAEDR
                     ECGIVEIARPVGVVAAITPSTNPGATPANNIINALKGRNAIVVAPSPKGYSTLALLLD
                     YVHAELDRVAAPRDLVQALPAPIRREQTFELMRQADLVVATGSQTNVKAAYSSGTPAL
                     GVGAGNVSVIVDETADVNAAAERIASSKTFDNATSCSSENAVIAVEAVYGRLVDALAA
                     QGGALLSPDETRALERTMFAGGKLSPAFIAQSAPAIAALAGFARPALEQARFLMVAET
                     GVGPEHPFSGEKLAPVLALYRVPDFAAAATLARRLLDYQGAGHSVGLHSRDENRALEL
                     GLTLPVCRVIVNQAHCFATGGSFDNALPFSLSMGCGTWGGNILSDNLNYRHFLNITSV
                     VRPLPAGAVHEPTEDELFGAWRRKFGT"
     gene            2106..>3057
                     /locus_tag="VHT22_07860"
     CDS             2106..>3057
                     /locus_tag="VHT22_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3137509.1"
                     /translation="MSPGAARFQTFRQAVDQLADEQPDATFLLAPESGTAVTYAELRR
                     MTRRFGALLRGLRVPEQGVVAFMLPNGLAAASIFLGSMTAGRIVSPINLLAQDAVLEY
                     TLGHAAPHAIFVAPEFFDRLSALAARIGLESAILSTDPDRLVLPDDETYDVASLESQR
                     PAMLMYTSGTTGKPKGVLLSHANMLHAGRTVSAAHALTPDDRVLSSLPLYHINGQCIG
                     VVSPLVSGGSIALPHRFSVSRWWPLVEHYRPTWLNVVPTIIAYLLHGPVPTPSQATAI
                     RGVRFARSASAPLPPEQHRAFEERFGVPVIEAMGLTECASV"
BASE COUNT          485 a         1028 c         1012 g          532 t
ORIGIN      
        1 ggcgatcgcc gaagagttcc cgaccacgaa gatcatcgcg ctggaggtgg acgccatcca
       61 gcatgcgcgc aatctcgcga tcaccgatct gcctaacgtg cgcttcgtcg cgggcggcgc
      121 agaagcgctg ccgctcgacg acgacagcgt ggacctggta ttgatgttca agtcgttgca
      181 tcacgtaccg aaccatcttc tcgatgccgc gctcgaggag gtgcgccgcg ttctcgtgcc
      241 gggcgggttg gcctacatct cggagccggt gttcgccggc gagtacaacg aaatcatgcg
      301 catctttcac gacgaggagc gcgtgcgcgg ggcggctttc tctgcgatta cgcgcagcgt
      361 ggaacgcggt gcgctggaat tcgtcaccga gcgttttttc aacacgccgc ttcgcttcga
      421 tgacttcgcc catttcgagg aacgcgtcct gggcgtgacc catacgcagc ataggctgtc
      481 ggaatcgcaa ctgagcgcgg tgcgcgatcg cttcgcgcgc cacgtaactc cgcagggcgt
      541 caaattcctg cagccgatgc gcgtcgatct gctgcgcaag ccggcgtgag tcgcgttcgc
      601 gactgctagc ttccgagcga gacggttgcg tcgggcgcag cgctgtggaa cacttcatcg
      661 gtgaaggcaa cgtcgatgca gcacgagaaa gtccggacac aagatgagcc ggcgacggta
      721 gttcgcgagc tcgtccggcg cgcgcgcgcc gcgcagcgtg tcgccgacgg ctggtcgcag
      781 gaacgcaccg atgaggtcgt catggccgcc ggctgggcga tcgtcgagcc agcccgcaat
      841 cgtgcgctcg cggagatcgc ggtccgcgac accaatttgg gcaacgtggc agacaagatc
      901 gcaaagaacc ggcgcaagac gatgggcctg ctgcgcgatc tgcagcacgc gcggtccgtc
      961 ggcgtgatcg cagaggatcg cgagtgtgga atcgtcgaga tcgcccgccc cgtcggggtc
     1021 gtggctgcaa tcacgccttc caccaacccg ggcgcgacgc cggcgaataa catcatcaac
     1081 gcgctcaagg gccgcaatgc gatcgtggtg gcgccgtcgc ccaagggcta ttcgaccctt
     1141 gcgctgctgc tggactacgt ccatgccgag ctcgaccgcg tggcggcgcc gcgcgatctg
     1201 gtgcaggcgc tgccggcgcc gataaggcgc gagcagactt tcgagctgat gcgccaggcc
     1261 gatctggtcg tcgcgacggg ctcgcagacc aacgtcaagg cggcgtactc gagcggaacg
     1321 ccggcgctgg gcgtcggcgc cggcaacgtc agcgtgatcg tcgacgagac ggctgatgtc
     1381 aatgccgccg ccgagcgcat cgcgagctcg aagactttcg acaacgccac cagctgctcg
     1441 tcggagaacg ccgtaatcgc ggtggaggcg gtctacggcc ggctcgtgga cgcgctcgcg
     1501 gcgcaaggcg gagctctgtt gtctcccgac gaaacgcggg cgctcgagcg caccatgttt
     1561 gccggtggca agctctcgcc ggcgttcatc gcccagtccg ccccggcgat tgcggccctg
     1621 gccggcttcg cgcggcccgc gctcgagcag gcgcgcttcc tgatggttgc agaaacgggc
     1681 gtcgggcccg aacatccctt ttcgggcgag aagctggcac cggtgctcgc gttgtaccgg
     1741 gtgcccgatt tcgccgcggc ggcgacgctc gcgcggcggc tgctcgacta tcaaggggct
     1801 ggccactcgg tcggcctcca ttcgcgcgac gaaaaccgcg cgctcgagct cgggctcacg
     1861 ctccccgtat gccgtgtcat cgtcaaccag gcgcactgct tcgcaaccgg aggcagcttc
     1921 gacaacgcac tgccgttctc gctgtcgatg ggttgcggta cctggggggg caatatcctc
     1981 tcggacaacc tgaactaccg ccacttcctc aacatcacga gcgtcgtgcg tccgctcccc
     2041 gccggggcgg tccacgagcc gaccgaagac gagctgtttg gcgcatggcg gcgcaagttc
     2101 gggacgtgag cccgggcgct gcgcgctttc agacgttccg gcaggccgtc gatcagcttg
     2161 cggacgagca gccggacgcg acattcctgc ttgccccgga gtccggcaca gcggtgactt
     2221 atgccgagct gcgccgtatg acgcgtcgct tcggcgcgct cctgcgcggc ttgcgcgtgc
     2281 ccgagcaggg cgtcgtggca ttcatgctgc ccaacgggct cgccgcggcc agcatcttct
     2341 tgggttcgat gacggccggt cgtatcgtat cgccgatcaa cctgctcgct caggatgccg
     2401 tgctcgagta caccctcggg cacgcggcgc cgcatgcgat cttcgtggcg cccgaattct
     2461 tcgatcggct gtccgcgctt gccgcgcgca tcggactcga gtcggcgatt ctatcgaccg
     2521 atcccgaccg cctcgtcctg ccggacgacg agacatatga tgttgcctcg ctggaaagcc
     2581 aaagaccggc gatgctgatg tacacctccg gcacgaccgg aaagcccaag ggcgtcctgc
     2641 tctcgcatgc aaacatgctg catgccggtc gtacggtcag cgcggcgcac gcattgacac
     2701 cggacgatcg ggtcctctcg tcgctgcctc tctatcacat caacggccag tgcatcggcg
     2761 tcgtcagccc gctggtttca ggcggcagca tcgcgctccc gcaccgcttc agcgtctccc
     2821 gatggtggcc tctggtcgag cattaccgcc cgacctggct caacgtcgtg ccgacgatca
     2881 tcgcgtacct gctgcacggg cctgtgccga cgccttcaca ggcgacggcg atccgtggcg
     2941 tgcgcttcgc gcgttcggcc tccgccccgc taccaccgga gcagcatcgc gcgttcgagg
     3001 aacggttcgg ggtaccggtg atcgaggcga tgggcctcac cgagtgcgcg tcggtgg
//
LOCUS       DATANS010000536         4744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_489994, whole genome shotgun sequence.
ACCESSION   DATANS010000536 DATANS010000000
VERSION     DATANS010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489994"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VHT22_07865"
     CDS             complement(<1..456)
                     /locus_tag="VHT22_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX3137510.1"
                     /translation="MTVRKGIILAGGSGSRLYPATQVLSKQLLPVYDKPMIYYPLSTL
                     MLAGIRDILLISTPEDTPRFTQLLGDGKRWGIDLRYAVQQTPNGLAQAFVIGRDFVGG
                     ASSALVLGDNIFYGHDLQPMLARANTRTAGATVFAYPVADPERYGVAEFD"
     gene            complement(453..1511)
                     /gene="rfbB"
                     /locus_tag="VHT22_07870"
     CDS             complement(453..1511)
                     /gene="rfbB"
                     /locus_tag="VHT22_07870"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX3137511.1"
                     /translation="MILVTGGAGFIGANFVLDWRAEGHEPVVNVDKLTYAGNLGSLAA
                     LKDDSGHHFVRADIGDRATMAALLAAHRPRAIVNFAAESHVDRSIHGPAAFIDTNIVG
                     TFALLETARAHWSSLPEPERSQFRFLHVSTDEVYGSLAPDADGFCETTRYAPNSPYSA
                     SKAASDHLVRAYHHTYGLPTLTTNCSNNYGPLQFPEKLIPLMIVNAIAGKPLPVYGDG
                     KNVRDWLYVGDHCAAIRTVLARGAPGATYNIGGNAEKTNIEVVHTLCRILADQRPGHD
                     YAAQISFVRDRPGHDRRYAIDSAKIRSELGWMPRETFESGLARTVRWYLDHAAWLQAV
                     SSGEYHKWISLNYTGEPA"
     gene            complement(1567..2562)
                     /locus_tag="VHT22_07875"
     CDS             complement(1567..2562)
                     /locus_tag="VHT22_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009303362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX3137512.1"
                     /translation="MTMLVTGAAGFIGMHLALRLARDGADVVGVDNFDPYYDVALKRA
                     RADQLARAGVRCIELDLADHAATEALFRDGRYTRVAHLAAQPGVRYSLQNPAAYIRNN
                     IDAFAAVIEACRHTGVEHLAYASSSSVYGANHTLPYSEDQNVDHPMSLYAATKKADEL
                     IAHSYSHLYRLPTTGLRFFTVYGPWGRPDMAAALFTRAIVDGTPVKVFNFGRMRRDFT
                     YIDDVVEGVVRVLSLPPVAADAGAAPYAIYNIGNHTAIDLESFIGELEQLLGKRAIRE
                     YLPPQPGDVPATFAAIDRIATATGFAPTTSLAEGLRRFVAWYLDYYRPGGPARAT"
     gene            complement(2559..3314)
                     /locus_tag="VHT22_07880"
     CDS             complement(2559..3314)
                     /locus_tag="VHT22_07880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020410675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3137513.1"
                     /translation="MPELSLVVITRDAGAQLAACLDSVRFAAETLVVDSGSKDDTVEI
                     ARARGARVIEQPWLGFGPQRRHAVAQAAHDWVLCLDADERLSPELAIAIGGALREPGH
                     NAFEMARRNRFFGRWLRHGEGYPDWTIRLFDRRHARWTDDVVHEHVVADGPVGRLAGD
                     LLHASAESLDAYLAKQNRYSTLQAEAMHARGEHFSWVRLIGSPLVRFVRFYLLRAGFL
                     DGSAGLVHIAIGCFTSFCKYAKLRGLERPADLR"
     gene            3496..4464
                     /gene="waaC"
                     /locus_tag="VHT22_07885"
     CDS             3496..4464
                     /gene="waaC"
                     /locus_tag="VHT22_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEX3137514.1"
                     /translation="MSRALIIRPSSLGDIVHALPIVHDIRRHRPGIAVDWVAEEAFVT
                     LLAMHRGVDRVIPVALRRWRHQVLDRATWREAAAFRVALTRDRYDLVLDLQEQVKGAL
                     LAWLARGPVHGPDRASIREPAASFSYRRRHRIDPAQHLIDRCRQLAAAAFGYPLDGPP
                     HFDLVPPPLVAPPETPYAIFVHASSRADKLWPEAHWRALIAHFTQAGLAVLLPWGTRE
                     EAERSGRLADGIPGAAVGARRELPEIAALLARAEIVCGVDTGLVHLAAALGAPTVALF
                     VATDPGLAGVARAGPRCRDLGGTGIVPKPSEVIAAVGALLRTLPDC"
     gene            4464..>4744
                     /locus_tag="VHT22_07890"
     CDS             4464..>4744
                     /locus_tag="VHT22_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HEX3137515.1"
                     /translation="MDFARALYTLAGATLLPLLPLRLWWRGRREPGYRHAWGERFGRY
                     RGEPPSSAVWWVHAVSVGETRAALPLLNHLRRAYPEVAIVLTHMTATGR"
BASE COUNT          766 a         1570 c         1619 g          789 t
ORIGIN      
        1 gtcgaattcc gcgaccccgt agcgctcggg atcggccacc ggataggcga acaccgtcgc
       61 cccggccgtg cgcgtgttgg cgcgcgcgag catcggctgg agatcgtgac cgtagaagat
      121 gttgtcgccc agcaccagcg ccgagctcgc gccgccgaca aagtcgcggc cgatgacgaa
      181 cgcctgcgcc agcccgttcg gcgtctgctg aactgcgtag cgcaagtcga ttccccagcg
      241 ctttccgtcg ccgagcagct gtgtgaaacg cggggtgtcc tccggcgtcg agatcagcag
      301 gatgtcgcga atgccggcca gcatcagcgt cgacagcgga tagtagatca tcggcttgtc
      361 gtacaccggc agcagctgct tggacagcac ctgcgtcgcc ggatagaggc ggcttcccga
      421 gccgccggcc aggatgatgc ccttgcggac tgtcatgccg gctcaccggt gtagttgagc
      481 gagatccact tgtggtattc gccgctgctc accgcttgca gccatgccgc atggtcgagg
      541 taccaccgca cggtgcgggc gagtcccgat tcgaatgttt cgcgcggcat ccagccgagc
      601 tcgcttcgga tttttgccga atcgatcgcg tagcggcggt catgccccgg gcggtcgcgc
      661 acgaagctga tctgcgcggc gtagtcgtgc cccgggcgct ggtcggcgag tatccggcaa
      721 agcgtgtgca cgacttcgat attggtcttt tcggcattgc cgccgatgtt gtacgttgcc
      781 cccggcgcac cgcgtgcgag caccgtgcgt attgccgcgc aatgatcgcc gacgtacagc
      841 cagtcgcgga cgttcttgcc atcaccgtag accggcagcg gcttgcctgc gatcgcattg
      901 acgatcatca gcgggataag cttttcggga aactgcagcg gtccatagtt gttcgagcag
      961 ttggtcgtaa gcgttggcag gccataggtg tgatggtaag cgcgcaccaa atgatcggat
     1021 gccgctttcg acgccgaata cgggctgttg ggcgcgtagc gggtcgtctc gcagaatccg
     1081 tccgcatcgg gcgcgagcga accgtagacc tcgtcggtcg aaacgtgcag aaagcgaaac
     1141 tgcgagcgct ccggttccgg cagtgacgac cagtgggcgc gcgccgtctc gagcagcgcg
     1201 aacgtgccga cgatgttggt atcgatgaag gcggccgggc catggatcga gcggtcgaca
     1261 tggctctcgg ccgcgaaatt gacgatcgcg cgcggccgat gcgcggccag caacgcggcc
     1321 atggtcgcgc gatcgccaat gtcggcgcgt acgaaatggt gcccggagtc gtccttgagc
     1381 gcggccagac tgccgagatt gccggcatag gtcagcttgt cgacgttgac cacgggctcg
     1441 tggccttccg cgcgccagtc gagcacgaaa ttggcgccga taaatccggc gccgccggtg
     1501 accagaatca tgcgagctcc ctgcgcccga aggcgagcga ggtgcttgcc cggggcgcgg
     1561 attctatcat gtcgcgcgag ctggtccgcc ggggcgatag tagtcgagat accacgccac
     1621 aaagcgccgc aatccctccg ctagcgatgt cgtcggcgcg aatcccgtcg ccgtcgcgat
     1681 gcggtcgatt gcggcgaacg tcgctggcac atcgcccggt tgcggcggca ggtattcgcg
     1741 gatcgcgcgc ttgccgagca attgctcgag ctcgccaatg aacgattcca ggtcgatcgc
     1801 tgtatggttg ccgatgttat agatcgcgta aggcgcggcg ccggcgtcgg cagcaacggg
     1861 tgggagcgag agtacgcgca cgacgccttc gacgacgtcg tcgatatagg tgaagtcgcg
     1921 acgcatccgg ccgaagttga acaccttgac cggcgtcccg tcgacgatcg cgcgcgtgaa
     1981 cagtgcggcc gccatgtcgg gccggcccca cgggccgtag acggtgaaga agcgcaagcc
     2041 ggtcgtaggc agccggtaca aatgactgta gctgtgcgcg atcagctcgt cggccttctt
     2101 ggtcgccgcg taaaggctca tcggatgatc gacgttctga tcctcggaat acggcagcgt
     2161 atggttggcg ccgtacacgc tcgagctcga tgcatacgcc aggtgctcga cgccggtatg
     2221 gcggcacgcc tcgatgactg cggcgaacgc atcgatgttg ttgcggatgt aggctgccgg
     2281 attctgcagc gaataccgca cgcccggctg cgcggccaga tgcgcaacgc gcgtatagcg
     2341 tccgtcgcga aacagcgcct ccgtcgccgc gtgatcggcc aggtcgagct cgatacaccg
     2401 cacgccggcg cgcgccaatt gatcagcccg cgcccgcttc agcgcgacgt cgtaataagg
     2461 atcgaagttg tcgacgccga cgacgtcggc gccgtcgcgc gccagccgca gcgcgagatg
     2521 catgccgata aatccggccg cgccggtaac cagcatcgtc atcgaaggtc cgccggccgc
     2581 tcgagccccc gcagcttggc gtacttgcag aagctggtga agcagccgat agcgatatgg
     2641 accagaccgg ccgagccgtc gagaaaaccg gcgcgcagca gatagaatcg cacgaagcgg
     2701 acgagcggcg agccgatcag ccgaacccag gagaagtgct caccgcgcgc gtgcatcgcc
     2761 tcggcctgta gcgtcgaata gcgattctgc ttggcgagat aggcatcgag cgactcggcc
     2821 gacgcgtgca gcaagtcacc ggcgagccgt ccgaccggcc cgtcggcgac gacgtgctcg
     2881 tggacgacgt cgtcggtcca gcgcgcgtgg cgacggtcga acagacggat cgtccagtcg
     2941 ggatagcctt cgccatggcg aagccagcga ccgaaaaagc ggttccgtcg cgccatctcg
     3001 aacgcgttgt ggcccggctc acgcaaggcc ccgccgatgg cgatcgcgag ctccggcgag
     3061 agccgctcgt cggcatcgag gcacagcacc cagtcgtgcg ccgcctgcgc caccgcgtgg
     3121 cgccgctgcg ggccgaatcc caaccacggc tgctcgatca cgcgcgcgcc gcgggcgcgg
     3181 gcgatttcta cggtatcatc cttgctgccg gaatcgacga ccagcgtttc cgcggcgaag
     3241 cgcacggaat cgaggcaggc agcaagctgc gcaccggcgt cgcgcgtgat caccacgagg
     3301 ctcaattcag gcaagggcaa gcggggctaa agatcggaca acgcgtcggg cgtcggggca
     3361 gaagagtctc cgatgcgaca tgcggcggag ggcgcggccc attctacgtg caacgcaccg
     3421 gcaagtcatc cgccggcctg ccacaatcaa cgcaccggcg gcattgcttg ctgcgatcct
     3481 cgtgaggcgt atcgggtgag tcgcgcgctt atcatcaggc cctcgtcgct cggcgacatc
     3541 gtgcatgcct tgccgatcgt gcacgacatc cggcgacatc gtcccggtat cgcggtcgac
     3601 tgggtcgccg aggaagcctt tgtcacgctg cttgccatgc atcgcggtgt cgaccgtgtc
     3661 attccggttg cgctgcgccg ctggcgccac caggtgctcg accgcgcgac ctggcgcgag
     3721 gccgcggctt tccgcgtcgc gctgactcgc gatcgttacg atctggtgct cgacctgcag
     3781 gagcaggtca agggggcgct gctggcgtgg ctcgcgcgcg gcccggtgca tgggcccgac
     3841 cgcgccagca tccgcgagcc tgcggcgagc ttcagctacc gtcgccgcca ccggatcgat
     3901 ccggcacagc atctgatcga tcgctgccgg cagctcgccg cggccgcgtt cggctaccct
     3961 ctcgacggac cgccgcactt cgacctggtg ccgccgccac tggtcgcgcc gcctgagacg
     4021 ccctacgcga tcttcgtgca cgcgagcagt cgagccgaca agctctggcc cgaggcgcac
     4081 tggcgcgcgt tgatcgcgca tttcacgcag gcgggcctcg cggtgctgct gccgtggggc
     4141 acccgcgaag aagccgagcg cagtggccgc cttgccgatg gcatcccggg cgccgccgtt
     4201 ggcgcgcgac gcgagctgcc ggagatcgcc gcgctgctgg cccgcgccga aatcgtctgc
     4261 ggcgtcgaca ccggcctggt acatctggcc gcggcgttgg gcgcgccgac ggtagcactg
     4321 ttcgtcgcca ccgatccggg gcttgccggc gttgcgcgcg ccggtccgcg ttgccgcgac
     4381 ctgggcggga cgggcatcgt tcccaagccg tccgaagtga ttgccgccgt cggcgcgttg
     4441 ctgcgcacac tgccggactg ctgatggact tcgcacgcgc gctgtacacg cttgccggcg
     4501 cgacgctgct gccgctgttg ccgctgcgcc tgtggtggcg cgggcgccgc gagcccggtt
     4561 accggcatgc gtggggcgaa cgtttcggtc gctatcgcgg cgagccgccg tcttctgcgg
     4621 tctggtgggt gcacgcggtg tcggtcggcg aaacgcgggc tgcgttgccg ctgctcaacc
     4681 atctgcgtcg cgcctacccc gaggtcgcca tcgtgctgac ccacatgacc gcaaccggcc
     4741 gcga
//
LOCUS       DATANS010000537         4022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_657433, whole genome shotgun sequence.
ACCESSION   DATANS010000537 DATANS010000000
VERSION     DATANS010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657433"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VHT22_07895"
     CDS             complement(<1..706)
                     /locus_tag="VHT22_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX3137516.1"
                     /translation="MLSVEDNELLCRVEGDAAMGQLMRRHWIPACLSEEVAERDGTPV
                     RVRLLGEDLVAFRDTAGRLGVLDEHCPHRRASLALGRNEECGLRCLYHGWKIDVEGNV
                     VDRPCEGHEAVLAKKIVHKTYPCREAGGFVWVWMGPPEQMRDFEPPAWAPSPSVRTSI
                     VKIHVGCNWAQVLEGAIDSAHSSSLHSTDMVPAQVASAGATEGEWLRPSTDKAPRLQV
                     QRTDFGFRYVAIRRPIE"
     gene            866..2065
                     /locus_tag="VHT22_07900"
     CDS             866..2065
                     /locus_tag="VHT22_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3137517.1"
                     /translation="MTEPRLRVGAAGLGRAFTLMLPTLTADPRVELVAGADPRPEARA
                     RFAAEFGGRIYASVEALCADAEVDVVYVATPHQFHADHVEAAAAGGKHVLVEKPMAIT
                     LDECRRMIDAVERANVRLIVGHSHSFDAPILRAREIIASGTLGAVRMINAQYYTDFMY
                     RPRRPEELVTAQGGGAVFNQAAHQVDIVRLLGGGLVRSVRAQTGAWDPARPTEGAYAA
                     LLVFDDGSYASLAYSGYGHFDGDELCGGVGELGTVKTDVDYGIARRKLRRAAGRDEEL
                     AFKNARNYGGVDYAEAGTGPETAPPSHQHFGLVLVCCERGDLRPMPDGVMIYGDGERH
                     LEPLPKPQVPRTEVIDEIYAAVVSGRTPLHGGRWAMATLEVCLSMLRSAREQRDVVLG
                     HQVGLPS"
     gene            2152..2727
                     /locus_tag="VHT22_07905"
     CDS             2152..2727
                     /locus_tag="VHT22_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3137518.1"
                     /translation="METADTDTLRLLKHWRDAVPDDRFAHLVKDATRALVRALQRRLA
                     PFGVPFGHWAFLRILWESDGLTQRELSREAGVMEPTTFGALKAMEIRGYIARRQLAGN
                     RRKIHIFLTRKGRALKNKLVPLAEEVNRIAVRGVPARDVASTRRTLLAVLESLAQDEA
                     RTAETPPPEGAASRPAARARRSRGKPRSALA"
     gene            2774..3766
                     /locus_tag="VHT22_07910"
     CDS             2774..3766
                     /locus_tag="VHT22_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3137519.1"
                     /translation="MARLQLSLACWHYDRTHALADGSVRPEGIDLNYLDMHVEETFFR
                     MLRNREFDVAEMSLSSYAVSVARDSPPFVAIPVFPSRFFRQSCIFVSEKSAIREPRDL
                     IGKRIGTPEYQMTAPVWIRGILQDEYGVDPASVEYCTGGEEEPGREEKLKLDLPAKFR
                     VTRIGAGKTLAAMLADGEIDALHAARIPSTFRSRAGTVRRLFENYVEVERAYYRKTRI
                     FPIMHTVAIRRDVYEANPWIAQSLTKAFVQAKQRVYENLYTTSALTTMLPWQIAQVEE
                     VRSDMGDDWWPYGFEANRHVLDTFLRYHHEQGLSPRRLQPEELFAPETLSSFRI"
     gene            complement(3779..>4022)
                     /locus_tag="VHT22_07915"
     CDS             complement(3779..>4022)
                     /locus_tag="VHT22_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020642649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3137520.1"
                     /translation="NALAPGIVATPLTRQIMSNAEWNRAYASRCALGRWAEPEEMAGP
                     IVFLASDAGSYVTGTIIFADAGWTAIDGRFEPRLNA"
BASE COUNT          669 a         1334 c         1375 g          644 t
ORIGIN      
        1 gctcgatcgg ccggcgtatc gcaacatagc ggaagccgaa gtcggtgcgc tgcacctgca
       61 gcctgggcgc cttgtcggtc gacgggcgca accactcgcc ctcggtcgcg ccggcgctcg
      121 ccacctgggc cggcaccatg tcggtcgaat gcaggctcga gctgtgcgcg gaatcgatcg
      181 cgccttcgag cacctgcgcc cagttgcaac cgacgtggat cttgacgatg ctggtgcgga
      241 ccgagggcga aggggcccac gccggcggct cgaaatcgcg catttgttcg ggcggcccca
      301 tccagaccca gacgaagccg ccggcctcgc ggcagggata ggtcttgtgc acgatctttt
      361 tggccagaac ggcctcgtgc ccttcgcagg ggcgatcgac gacgttgcct tcgacgtcga
      421 ttttccagcc gtggtacagg cagcgcaggc cgcattcctc gttgcggccc agcgcgagcg
      481 acgcgcgccg atgcgggcaa tgctcgtcga gcacgccgag gcggccggcg gtgtcgcgaa
      541 acgccaccag atcctcgccc agcagccgca cgcgcaccgg cgtcccgtcg cgctcggcga
      601 cctcctcgga cagacacgcc ggtatccagt ggcggcgcat cagctggccc atcgccgcat
      661 cgccctcgac ccggcaaagc aactcgttgt cttcgacgct caacatgctt acctccggca
      721 gcgggttcga gccgacgcgg ttgcccgagt cggcgcaacg ggcataatat tagggcccta
      781 agtaaataat agggaatcgg atgcgcgatg tcgagcattg cagcccggtc gggcgatgcc
      841 ggcccatacc gccgggcgat ttcgcatgac tgagccgaga ttgcgggtcg gcgccgcagg
      901 actcggtcgc gcgttcacgc tgatgctgcc gacgttgacc gcggacccgc gggtcgagct
      961 ggtcgccggc gccgatcctc gcccggaggc gagagcgcga tttgccgcgg aattcggcgg
     1021 ccgcatctat gcgtcggtcg aagcgctgtg cgccgatgcc gaagtcgatg tcgtgtacgt
     1081 cgccacgccg caccagttcc acgccgacca cgtcgaggcg gcagccgccg gcggcaagca
     1141 cgtgctggtc gaaaagccga tggcgatcac gctcgacgag tgccgccgca tgatagacgc
     1201 ggtcgaacgc gccaatgtcc gcctgatcgt cggccacagc cacagcttcg acgcgcctat
     1261 cctgcgcgcg cgcgagatca tcgccagcgg aacgctcggc gccgttcgca tgatcaacgc
     1321 gcagtactac accgacttca tgtaccggcc gcgccggccg gaagaactgg tcacggcaca
     1381 gggcggcggc gcggtcttca accaggccgc ccatcaggtc gacatcgtgc ggctgctcgg
     1441 cggcggactg gtgcgtagcg tgcgcgccca gaccggtgcg tgggacccgg cgcgcccgac
     1501 cgagggtgcg tatgccgcgc tgctggtgtt cgacgacggc agctatgcgt cgctggcgta
     1561 cagcgggtat gggcatttcg acggcgacga gctttgcggc ggcgtcggcg aactggggac
     1621 ggtgaagacc gacgtcgatt acggcatcgc gcgccggaag ctgcgccgcg ccgccggtcg
     1681 cgacgaggaa ttggcattca agaatgcgcg caactacggc ggcgtcgatt acgccgaagc
     1741 cgggaccggg ccggagaccg cgccgccctc gcaccagcat ttcggcctgg tgctggtctg
     1801 ttgcgagcgc ggcgatctgc gtccgatgcc ggacggcgtg atgatctacg gtgacggcga
     1861 gagacacctg gagccgctgc cgaagccgca ggtgccgcgc accgaagtca tcgacgagat
     1921 ctacgccgcc gtcgtttccg ggcggacgcc gctacacggc ggccggtggg cgatggccac
     1981 gctggaagtg tgtctgtcga tgctgcgatc ggcgcgcgaa cagcgcgatg tcgtgctcgg
     2041 tcatcaggtc ggtctgccgt cgtgactggc gttcgctcac gcgcgcgccc ggcgccgctg
     2101 gcggtgaagc ggcgcgccgg ccgcatccga gcgcagccgg ccggtgcaac gctggaaacc
     2161 gccgacaccg atacgctgcg cctgctcaag cattggcgcg atgccgttcc cgacgatcgc
     2221 ttcgcacacc tggtaaagga tgcgacccgc gcgctggttc gggccttgca gcggcggctt
     2281 gctccgttcg gcgtgccgtt cggccattgg gcgttcctgc gcattctctg ggagtccgac
     2341 ggactgaccc agcgcgaact cagtcgcgag gcgggggtga tggagccgac aacgttcggt
     2401 gcgctgaagg cgatggaaat ccggggctat atcgcgcgac gccagctcgc aggcaatcgg
     2461 cgaaagatac atatttttct cacccgcaaa gggcgcgcgc tcaaaaacaa actggtgccg
     2521 ctggcggaag aggtgaaccg catcgccgtg cgcggcgtac cggcgcgcga cgtcgcgtcg
     2581 acgcggcgaa cgctgctcgc cgtgctcgaa agccttgcgc aggacgaagc gcggaccgcc
     2641 gagactccgc cgcccgaggg cgctgcatcc cgcccggcgg cgcgagcgcg gcgttcgcgc
     2701 ggcaagcccc gaagcgcgtt ggcgtagagt gtcgccggcg cggtccgact gcggatttca
     2761 cccaaggaac accatggccc gactgcagct ctccctggcg tgctggcact acgatcgcac
     2821 tcacgcgctc gcggacggca gcgtgcggcc cgaggggatc gatctcaact atctcgacat
     2881 gcacgtcgag gagacgttct tccgcatgct gcgcaaccgc gaattcgatg tcgccgaaat
     2941 gtcgctgtct tcgtatgcgg tgtcggtcgc gcgcgattcg ccgccgttcg tcgcgatccc
     3001 ggtctttccc tcgcgtttct tccggcagtc gtgcatcttc gtgtccgaaa agagcgcgat
     3061 acgcgagccc cgcgacctga tcggcaagcg catcggcacg cccgaatacc agatgacggc
     3121 gccggtatgg atccgcggca ttctgcagga cgaatacggc gtcgatccgg ccagcgtcga
     3181 atactgtacc ggcggcgagg aagagccggg acgcgaggaa aagctcaagc tcgatctgcc
     3241 ggcaaaattc agggtgacgc gcatcggcgc gggaaaaacg ctggcggcga tgctcgccga
     3301 cggcgagatc gacgccttgc atgcggcgcg catcccatcg accttccgca gccgggcggg
     3361 caccgtccgg cgcctgttcg agaactacgt cgaggtcgag cgggcctact accgcaagac
     3421 ccggatcttc ccgatcatgc acacggtggc gatacgccgc gacgtgtacg aggccaatcc
     3481 ctggattgcg caatcactga ccaaggcgtt cgtgcaggcg aaacagcggg tctacgagaa
     3541 tctttataca acgtcggcgc tgacgacgat gcttccctgg cagatcgcgc aggtcgagga
     3601 ggtgcggagc gacatgggtg acgactggtg gccttatggc ttcgaggcga accgccatgt
     3661 gctcgacacg ttcctgcgct accaccacga gcagggattg tcgccgcgcc ggctgcagcc
     3721 ggaagagctg ttcgctccgg agaccctgtc ctccttccgg atctaggatg ccggcacttc
     3781 aggcgttgag ccgcggctcg aagcggccgt cgatcgcggt ccagccggca tcggcgaaaa
     3841 tgatcgtgcc ggtcacgtag ctgccggcgt cggaggcgag gaagacgatc ggcccggcca
     3901 tttcctcggg ttcggcccag cggccgagcg cgcatcggct ggcataggcg cggttccatt
     3961 cggcattcga catgatttgg cgtgtcaacg gcgtcgcgac gatacccggc gccaatgcat
     4021 tg
//
LOCUS       DATANS010000538         1856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_196918, whole genome shotgun sequence.
ACCESSION   DATANS010000538 DATANS010000000
VERSION     DATANS010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196918"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /locus_tag="VHT22_07920"
     CDS             <1..117
                     /locus_tag="VHT22_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX3137521.1"
                     /translation="DEVAYVRFASVYRSFQDVADFRDALQEVEPAPTRRKPR"
     gene            164..1255
                     /gene="ribD"
                     /locus_tag="VHT22_07925"
     CDS             164..1255
                     /gene="ribD"
                     /locus_tag="VHT22_07925"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX3137522.1"
                     /translation="MSRFDDHDRAFMTRALELAKLGLYTTTPNPRVGCVLVHDGYIIG
                     EGWHQRAGGPHAEVHALADAAARGADARGATVYVTLEPCNHQGRTPPCTDALLAAGVA
                     RVVAAMADPYPPAAHGAERLRAAGIEVDIGLFEEDARELNPGWLMRLEHGRPWVRVKI
                     AASLDGRTALANGASQWITGEEARADGHRWRARACAILTGIGTVQQDDPQLTVRGVAT
                     PRQPLKVIVDRHGELPPNARVLADGEVLVVTASTPRAVWPPAVRTMNLPDRDGRIDLA
                     AMMAALAAEGINEVHVEAGARLNGALLGAGLVDELLVYLAPCLLGDGARGMFALPAPL
                     ERLADRTPLRIVGVDPVGSAWRVLARIER"
     gene            1255..1854
                     /locus_tag="VHT22_07930"
     CDS             1255..1854
                     /locus_tag="VHT22_07930"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX3137523.1"
                     /translation="MFTGIVQAVGLIATAQAMSDGLKLTLDAGTLGLSDLGVGDSVAV
                     SGCCLTIVSIDQTKLTFDVSAETLRCTARLDGPGPVNLEKALRLSDRLGGHLMAGHVD
                     GVGIVTRFERAASDGENVELEIEAPADLARFIASKGSIAVDGVSLTINAVAGRRFSVN
                     LIPHTLAVTTLRSLVPGRRVNLEVDLIARYVARYAKATT"
BASE COUNT          277 a          641 c          651 g          287 t
ORIGIN      
        1 gacgaggtcg cctacgtgcg gtttgcgtcg gtgtaccgca gcttccagga cgtcgccgac
       61 tttcgcgacg cgttgcagga agtcgagccc gcgccgacgc ggcgcaagcc ccgctgagca
      121 cgtcgccgca gcggcggcga ctcgcgcaga ttttgaccca ggcatgagcc gtttcgacga
      181 tcacgatcgc gcgttcatga cgcgcgcgct cgagctcgcc aagctcggcc tctacacgac
      241 tacgcccaac ccgcgcgtcg gttgcgtgct cgttcacgac ggctacatca tcggcgaagg
      301 ctggcatcag cgcgccggcg ggccgcacgc cgaagtccac gcactggccg atgctgccgc
      361 gcgcggtgcg gatgcgcgcg gcgcgactgt ctacgtcacg ctggaaccct gcaaccacca
      421 aggcagaacg cctccctgca ccgacgcctt gctcgctgcc ggcgtggcgc gggtggtcgc
      481 cgcgatggcc gacccgtatc cgccggcagc gcacggcgcc gagcgcctgc gcgcagccgg
      541 aatcgaagtc gacatcggtt tgttcgagga agatgcgcgc gagctcaatc ccggctggct
      601 gatgaggctc gagcacggcc gcccctgggt gcgggtcaag atcgcggcca gcctcgatgg
      661 ccggaccgcg ctcgcgaatg gcgccagcca atggatcacc ggcgaggaag cgcgtgccga
      721 cggccatcgc tggcgggcga gggcgtgcgc gatcctgacc ggcatcggca ccgtccagca
      781 agacgacccg cagctcacgg tgcgcggggt cgccacgccg cgccagccgt tgaaagtcat
      841 cgtcgaccgc catggcgagc ttccgccgaa tgcgcgggta ttggccgatg gcgaggtgct
      901 cgtggtcacc gcatcgacac cgcgggcggt ctggccgccg gcggtccgca cgatgaatct
      961 gcccgatcgc gacggccgca tcgacctcgc ggcgatgatg gcggcgcttg ccgcggaagg
     1021 catcaacgag gtccacgtcg aggccggcgc ccggctgaac ggcgctttgc tcggcgccgg
     1081 gctggtcgac gagctgctgg tctacctggc gccgtgcttg cttggcgacg gcgcgcgcgg
     1141 catgttcgcg ctgccggcgc cgctcgagcg cctggccgat cgcacgccgc tgcggatcgt
     1201 cggcgtcgac ccggtcggca gcgcctggcg cgtcctcgcc aggatcgaac gctgatgttc
     1261 accggcatcg ttcaggcagt cggcctcatc gccacggcgc aggcgatgtc ggatgggctc
     1321 aagctcacgc tggacgccgg tacgctcggc ctgtcggacc tcggcgtggg cgacagcgtt
     1381 gcggtcagcg gctgctgtct gacgatcgtc tccatcgacc agaccaagct cacctttgac
     1441 gtgtcggcgg agacattgcg ctgcacggca cggctcgatg gccccggccc ggtcaatctg
     1501 gaaaaagcgc tgcgactttc ggaccggctc gggggtcatc tgatggctgg ccacgtcgac
     1561 ggggtaggca tcgtgacgcg cttcgagcgc gcggcgagcg atggcgagaa cgtcgagctc
     1621 gagatcgagg cgccggcgga ccttgcgcgc tttatcgcca gcaagggctc gatcgccgtc
     1681 gacggcgtca gcctcacgat caatgcggtc gcggggcggc gcttttcagt caatctgatc
     1741 ccgcatacac tggctgtcac gacgctgcgc tccctcgttc cgggacggcg ggtcaacctc
     1801 gaggtcgacc tgatcgcgcg gtacgtcgcg cgatacgcga aagccacgac gtagga
//
LOCUS       DATANS010000539         3032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_405915, whole genome shotgun sequence.
ACCESSION   DATANS010000539 DATANS010000000
VERSION     DATANS010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405915"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VHT22_07935"
     CDS             complement(<1..192)
                     /locus_tag="VHT22_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137524.1"
                     /translation="MLILALIAVVAIAALTGYAYGLRRRQLPAGTPTGSTVTGAVDPA
                     GAFSGPDAERIGAEKARIIR"
     gene            449..1396
                     /locus_tag="VHT22_07940"
     CDS             449..1396
                     /locus_tag="VHT22_07940"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX3137525.1"
                     /translation="MIAAAITAAWLDARHGQRELRVDLAKRLAEVESAQQATRAIVKD
                     AQDSVRDAQAKIALLENRLGESQAQQSALEALYRELSPSRDEWALTEVEQVLMLASQQ
                     LQLAGNVRAALAALQVADTKLQRLDRPQFVPLRRALARDMDRLKAVPYVDVAGVSLRL
                     DEAIAQIDALPLAIDERLAPPPPPAPQQGESGWRRLLASAWQQIRQLVRIENIDRPEV
                     PLLLPQEQFFLRENLKLRLLSARFALLFHDQASFRADLTAADAWLAKYFDTRSKPVQA
                     LQTLFKQLKATDMAAELPGLAASLDAVRVLQIARDKPQR"
     gene            1433..2623
                     /locus_tag="VHT22_07945"
     CDS             1433..2623
                     /locus_tag="VHT22_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HEX3137526.1"
                     /translation="MRFLFWFLLLAAAAVAAALAVKLNAGYALLVTPPYRIELSLNLL
                     LLIAVVGFAALYAAIRLAVRTARLPQTVRAWRRRQKLERARSAQDSAVVALLEGRYGK
                     AQQRALEALAIPGSSGLNAIIGARAAIDVRNFDSAEAMLSRPDAQATSLAVARLMLLA
                     EIALEQGQPQHALDTLRELRREAGMHTAALRLELRCLQAARRWTDIAPLLDQLVRRKV
                     FDSTQAEHVRVTAQSEQLKVLTHDAAGLREYWNRLPDAAKRHPKVARAAAQSFLQLGG
                     DREAVDILTESLEREWNSELVEIYGECRPADATRQLEQAERWLLRHNQDPVLLRVLGT
                     LCQRRQLWGKAQTYFEASLALDDGWRAHLALGEMLGRLNRDDEANAHFVAALKLATGE
                     LRRF"
     gene            complement(2637..>3032)
                     /locus_tag="VHT22_07950"
     CDS             complement(2637..>3032)
                     /locus_tag="VHT22_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137527.1"
                     /translation="HQPNLAFIRASRVADLVRYYREEPPTDARTWLHDLDAVSAWVEK
                     IQRRGGRVVFFREPAADESLALDEANFPRDRYWDAYARRAPATMIDFRDVPEMKAFVL
                     PDTSHIDATDVPRFTIALANLLKSMKLVD"
BASE COUNT          505 a         1001 c         1038 g          488 t
ORIGIN      
        1 gcgtatgatc cgcgcttttt ctgcgccgat acgctcggcg tcaggcccgg aaaacgcgcc
       61 cgctggatcg actgcaccgg ttaccgtaga gccggtcggc gtgccggccg gcagctgacg
      121 ccggcgcaat ccatacgcgt aaccggtcag cgcggcgatg gcaacgacag cgatgagtgc
      181 aaggattagc atcggatcgg aaaaggcggg ggccgcggga cgataaacgc ctggcggacg
      241 tgcgaacatt ctagtggagc gtggcgcttc cggccagccc ggcagggagc gaccggccgt
      301 cctgctacaa tcgactgcgt ctgcgctcgc ctttactacg atgcccgaac cggcggccga
      361 ccgcccagct tcctcctcgc cgccgccccg aatcccggcc actgcggccg cgccgcggct
      421 tgcactctgg gcgtgggttg cagcgctggt gatcgctgct gcgatcacgg cagcgtggct
      481 ggacgcacgc cacggccagc gcgagttgcg cgtcgatctc gcgaagcgtc tcgccgaagt
      541 cgaaagcgcc cagcaggcaa cgcgcgccat cgtcaaggat gcccaggaca gcgtgcgtga
      601 cgcgcaagcc aagatcgcgc ttctggaaaa ccgtctcggc gaatcgcagg cgcagcagtc
      661 ggcactcgag gcgctgtacc gcgagctttc gccgtcgcga gacgaatggg cactgaccga
      721 ggtcgagcag gtgctgatgc tggccagcca gcagctgcaa ctggccggga acgtccgcgc
      781 tgcgcttgca gcgctgcagg tcgccgacac caaattgcag cggctcgacc gcccgcaatt
      841 tgtccctctg cgccgcgcgc tggcgcggga catggaccgg ctgaaggccg tgccgtacgt
      901 cgacgttgcg ggagtgtcgt tgcgactcga cgaggccatc gcgcagatcg atgcgctgcc
      961 gctggcgatc gatgaaaggc tggccccgcc gccgccgccc gcgccgcaac agggcgagtc
     1021 cggatggcgc cgactgctgg cgagcgcatg gcagcagata cggcaactgg tccgtatcga
     1081 gaatatcgac cggcccgagg tccccctgct gctgccgcag gagcagtttt tcctgcgcga
     1141 gaatctgaag ctgaggctcc tgtcggcccg attcgcgctg ctgtttcacg atcaggcgag
     1201 cttccgcgcc gatctcactg ccgccgacgc gtggctcgcc aagtatttcg atacgcgatc
     1261 caagccggtt caggcgttgc agacgctgtt caaacagctg aaggcgaccg acatggcggc
     1321 cgagctgccg ggactcgccg ccagcctcga tgcggtgcgc gtgctgcaaa tcgcgcgtga
     1381 caagccgcag cgctagagcg gcaacaggcc actagcgccg cgcccgccgc caatgcgctt
     1441 tcttttctgg tttttgctgc tcgccgcggc ggcggtggcc gccgcgctgg cggtcaagct
     1501 gaatgccggt tacgcgctgc tggtcacgcc gccgtaccgg atcgagcttt cgctcaacct
     1561 gttgctgttg atcgccgtcg tcggcttcgc cgccttgtat gctgcgatcc gcctggccgt
     1621 gcgcaccgcg cgtcttccgc agacggtgcg cgcgtggcgc cggcgccaga agctcgagcg
     1681 ggcccgttcg gcgcaggact ccgccgttgt cgcattgctc gaagggcgtt acggcaaggc
     1741 acagcagcgt gccctggaag cgctggccat accgggttca agcggactca acgcgattat
     1801 cggcgctcgc gccgccatcg acgttcgcaa tttcgattcg gccgaggcga tgctcagtcg
     1861 cccggacgcg caagcgacga gcctggcggt cgcccggttg atgctgcttg ccgaaatcgc
     1921 gctcgagcaa ggacagccgc aacacgcgct cgacacgttg cgcgagctgc ggcgcgaagc
     1981 cggcatgcat acggcggcgt tgcgcctgga gctgcgctgc ctgcaagcag cgcggcgctg
     2041 gaccgacatc gcgccgctgc tcgatcagct ggtccggcgc aaagtgttcg acagcacgca
     2101 agccgagcat gtgcgagtga cggcgcaaag cgagcagctg aaggtgctga cgcatgacgc
     2161 cgctgggttg cgcgaatact ggaacaggct gccggatgca gcgaagcggc atccgaaagt
     2221 cgcgcgcgcc gcagctcaga gcttcctgca gctcggcgga gatcgggagg ccgtcgacat
     2281 cctcaccgag agtctggagc gcgaatggaa ctccgagctg gtcgagatct acggcgaatg
     2341 ccgtccggcc gacgccaccc gccagctcga acaggctgaa cgctggctgc tgcggcacaa
     2401 ccaggatcca gtgctgttga gagttctcgg cacgctgtgc cagcggcggc aactctgggg
     2461 caaggcgcaa acctatttcg aggccagcct cgcgctcgac gatggctggc gcgcgcatct
     2521 ggcgctcgga gaaatgctcg gacgcctgaa ccgcgacgac gaggcgaatg cgcatttcgt
     2581 cgcggcgctc aagctggcga ccggcgaact gcggcggttc tgacggttgt cgggctttag
     2641 tcgacgagct tcatgctttt caggaggttc gccagcgcga tcgtgaagcg aggcacgtcg
     2701 gtggcgtcga tgtgcgaggt gtccggcagc acgaacgcct tcatctcggg gacgtcgcga
     2761 aagtcgatca tcgttgccgg cgcgcgccgc gcatacgcgt cccagtagcg gtcgcgcgga
     2821 aaattggcct cgtccagcgc caggctctcg tcggccgccg gctcgcgaaa aaagacgacg
     2881 cggccgccgc gccgctggat tttctcgacc cacgccgata cggcgtccag gtcgtgcagc
     2941 caggtccggg catcggtcgg cggctcctcg cgatagtagc gaaccaggtc ggcgacgcgc
     3001 gatgcccgga tgaaggcaag attgggctgg tg
//
LOCUS       DATANS010000540         3018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_612466, whole genome shotgun sequence.
ACCESSION   DATANS010000540 DATANS010000000
VERSION     DATANS010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612466"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..>3018
                     /locus_tag="VHT22_07955"
     CDS             145..>3018
                     /locus_tag="VHT22_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX3137528.1"
                     /translation="MNSALERPVAIDGRLKRAFDSSLLARRLREACRGDVLFDAASRG
                     RYATDASIYQIDPIGVVVPCDEDDVRDALAVARELAVPVLPRGAGSSQCGQTVGDALV
                     IDHSKSLCRILAFDDDTMTATVQPGVVLDQLNAFLRPHGVWFPVDVSTSAQATIGGMA
                     GNNSCGSRSIEYGNMVHNVAGIDAILADGSEIAFGPIDRMRRDGRCGEIVAGLRRIAA
                     AERDEIERSVPKVLRRVGGYNLDLFAPQSVRPYTADGSVNLAHLLVGSEGTLAWSSRL
                     TLALAPLPRHKALGVVNFPSFYQAMAAAQHIVRLKPTAVELVDRTMVDLARANPAFRP
                     VIERALIGAPAAILLVEFSGEDAAAQAASLDRLAELSGDLGLPDAVVRIESEQAQKAL
                     WEVRKAGLNIMMSMKGDGKPVSFIEDCAVPLEHLAEYTDRLTEVFRKHGTTGTWYAHA
                     SVGTLHVRPILDMRRDGAAKMRAIAEEAAAMVREYKGAYSGEHGDGLCRGEWVAWQFG
                     PRLNAAFGAVKVLFDPDNRMNPGKIVATPKMDDTSLFRFRPQYGRIPIQPKLDWSAWN
                     VERDPATGDESAPGTGSDVTQGLAKAVEMCNNNGHCRKFDAGTMCPSFRVTRDEQHTT
                     RGRANTLRLALSGQLGEEDLGGEAVHAALDLCVSCKACRRECPTGVDMAKMKIEALAA
                     YNQRHGVALRDRLIAHLPRYAPMVSRIAWLANLLTRLPGAAQLAESWLGLAARRRLPP
                     WRGGFLRRTRSDDAHDASEREAVLFVDTFSNYFAHAAAADARRVLEVAGYTVHTNAVP
                     GERPLCCGRTFLAAGMIDEAKAEARRTLAALVPYARRGVAIVGLEPSCLLGMRDEFLN
                     YGLGEDAELVARHAMLFEEFLVRERAAGRLTLPLKPLPQSEALLHGHGHQKAFAVMTP
                     IQTALGWIPGLATTTIASGCCGMAGAFGYHAEHHPLSMQMAEQA"
BASE COUNT          509 a          977 c         1044 g          488 t
ORIGIN      
        1 tggtcgtcga gtttcagcat gcgcctggtc gattgcgctg gaatccgatc gctggaaatc
       61 gccatggcca ttatcgaagc catcgccggc gaggtatagt cgcattgtac aactgcgatc
      121 ccgattctaa ggcaaatcct caagatgaac tccgcgctgg aacgaccggt cgcgatcgac
      181 ggccggctca agcgtgcctt cgactcgtcg ttgctcgcga gacggctgcg cgaggcgtgc
      241 cgcggcgatg tgctgttcga cgcggcctcg cgggggcgct atgcgaccga cgcttcgatc
      301 taccagatcg acccaatcgg ggtcgtcgtg ccgtgcgacg aggacgacgt ccgggacgca
      361 cttgccgtcg cgcgcgagct tgccgttccg gtgttgccgc gcggcgcggg ctcatcgcaa
      421 tgcgggcaga cggtcggcga tgcgctggtg atcgatcaca gcaagtcgct gtgccgcatt
      481 ctggcgttcg acgacgacac gatgacagcc accgtgcagc ccggcgtcgt tctcgatcag
      541 ctgaacgcct tcctgcggcc gcacggcgtc tggttcccgg tcgacgtcag cacgtcggcg
      601 caggccacga taggcggcat ggcgggcaac aactcctgcg gctcgcgatc gatcgaatac
      661 ggcaacatgg tgcacaacgt cgccggcatc gatgcgatcc tggccgacgg cagcgagatc
      721 gcattcgggc cgatcgatcg gatgcgccgc gacggccgct gcggcgagat cgtcgccggg
      781 ctcaggcgaa tcgccgcggc cgagcgcgac gagatcgagc gcagcgtgcc gaaggtgctg
      841 cgccgggtcg ggggctacaa cctcgatctc tttgcgccgc aaagcgtgcg tccgtacacc
      901 gccgatgggt cggtcaatct cgcccatctt ctggtaggct ccgaaggcac gctcgcctgg
      961 tcgtcgcgac tgacgctggc gctcgcgccg ctgccgcggc acaaggcgct cggcgtggtc
     1021 aactttccgt ctttctacca ggcgatggcg gcggcgcagc acatcgtgcg cctcaagccg
     1081 actgcggtcg agctggtcga tcgcacgatg gtcgatctgg cgcgcgcgaa tcccgcattc
     1141 cgtcctgtga tcgagcgcgc attgatcggt gcgcccgcgg cgatcctgct ggtcgagttc
     1201 tccggcgaag atgccgccgc gcaggcggcg agtctcgatc gtctggccga gctgagcggc
     1261 gatctcggcc tgccggatgc ggtcgtgcgg atcgaaagcg agcaggcgca gaaggcattg
     1321 tgggaagtgc gcaaggcggg cctcaacatc atgatgtcga tgaaaggcga cggcaagccg
     1381 gtctcgttca tcgaggactg cgcggtgccg ctcgagcatc tggccgaata caccgacagg
     1441 ctgaccgaag tgttccggaa acacggcacc accgggacct ggtacgcgca cgccagtgtc
     1501 ggcaccttgc acgtgcggcc gatcctcgac atgcgtcgcg acggcgcggc caagatgcgc
     1561 gcgatcgccg aagaagccgc ggcgatggtg cgcgaataca agggtgcgta ttcgggcgag
     1621 catggcgacg gcctgtgccg aggcgaatgg gtggcctggc agttcggacc gcgcttgaac
     1681 gccgcgttcg gcgcggtcaa ggtgctgttc gacccggaca accggatgaa ccccggcaag
     1741 atcgtcgcta cgccaaagat ggacgatacc tcgctgttcc gcttccgacc gcagtacggc
     1801 aggattccga tccagccgaa gctcgactgg tcggcatgga acgtcgagcg cgatccggcg
     1861 accggcgacg aaagcgcgcc cgggaccggc agcgacgtca cgcagggtct cgccaaggcc
     1921 gtcgagatgt gcaacaacaa cggtcactgc cgcaagttcg acgccggaac gatgtgcccg
     1981 agctttcgtg tcacgcgcga cgagcagcat acgacgcgcg ggcgcgccaa cacgctgcgc
     2041 ctcgcactgt cgggacagct tggcgaggaa gacctgggcg gcgaagccgt gcatgcggcg
     2101 ctcgacctgt gcgtctcgtg caaggcctgc cgccgcgaat gcccgaccgg cgtcgacatg
     2161 gcgaagatga agatcgaagc gctggcagcc tacaaccaac ggcacggcgt cgcgctgcgc
     2221 gaccggctga tcgcgcatct gccgcggtat gcgccgatgg tctcgcgcat cgcatggctt
     2281 gcgaacctgc tgacgcgtct tccgggcgcc gcgcagctcg ccgaatcctg gctcgggctt
     2341 gcggcgaggc gccgcttgcc gccgtggcgc ggcggcttcc tgcgccggac gagatcagac
     2401 gacgcccacg acgcgagcga acgtgaagcc gtgctctttg ttgacacgtt cagcaattac
     2461 ttcgcgcacg cggccgcggc cgacgcgcgt cgcgtgctcg aggtcgctgg atacacggtg
     2521 cacaccaatg cggtgccggg cgaacgcccg ctgtgctgcg gacgaacctt tctcgctgcg
     2581 gggatgatcg acgaggcgaa agccgaggcc cgccgcacgc tggcggcgct ggtcccgtac
     2641 gcgcgtcgag gcgtggcgat cgtggggctc gagccgtcgt gcctgctcgg aatgcgcgac
     2701 gaattcttaa actacggcct aggggaggat gcggagctcg tcgcgcgcca cgcgatgctg
     2761 ttcgaagagt tcctggtgcg ggagcgcgct gccggacgcc tgaccttgcc attgaagccg
     2821 ctgccgcaaa gcgaggcgct gctgcacgga catggccatc aaaaagcctt cgccgtaatg
     2881 acgccgatcc agacggcgct cggctggata ccgggtctgg cgacgacaac gattgcctcg
     2941 ggttgctgcg gcatggccgg cgcgttcggc taccacgcgg agcatcaccc gctgtcgatg
     3001 caaatggcag aacaggcg
//
LOCUS       DATANS010000541         3626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_657651, whole genome shotgun sequence.
ACCESSION   DATANS010000541 DATANS010000000
VERSION     DATANS010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657651"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1904)
                     /locus_tag="VHT22_07960"
     CDS             complement(54..1904)
                     /locus_tag="VHT22_07960"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX3137529.1"
                     /translation="MAEAIALAIKTRGQLLAEAGTGTGKTFAYLVPALLSGGKVIIST
                     GTKTLQDQLYQRDLPLVRDALRIPITVALLKGRGNYVCHHHLARTAAQPTFASRADIG
                     YVKRIESFVRTSASGDKSEVADVPENAAIWADVTSTRENCLGAECGHYNECFVMNARK
                     QALAADVVVINHHLFFADVMLKDEGIAELLPASNTVILDEAHQLPETATLFFGEEVTG
                     GQLGELARDAELEGLKAARDYAPLPAAAQALGLALRRFRLAAGETPGKLPRALAMRRP
                     GFDEALDALDAALAAVAVELGKQAERSDDLAQCAHRADDALARLARWRDHDDPEWIRW
                     LDVSASGWQLRASPLSVAEIFAKQVTDHARAWIFTSATLAIGSDFSLYQRELGLGEAA
                     TGYWESPFDYARQALLYLPRELPEPNSFEHTDAVVAAALPVLKASGGRAFLLFTTLRA
                     LARARESLEALFKREGIDFPLLVQGEGSRTELLTRFRRLGNAVLLGSQSFWEGVDVQG
                     EALSVVIIDKLPFAPPDDPLLAARLEHIARQGGNPFFDYQLPQAAISLKQGAGRLIRS
                     ETDRGVLMICDRRLVDKPYGRRIWQALPPMRRTRELADVRAFFAPVAVEG"
     gene            complement(2056..2595)
                     /locus_tag="VHT22_07965"
     CDS             complement(2056..2595)
                     /locus_tag="VHT22_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137530.1"
                     /translation="MLRLALLALLAVSAVLPAASVYALDNVNPYICPTAFNGSGTDCF
                     FEAVPHTYAMCHLVKSIEIMEFGLAGAQQGVNGAKTEGCIEKNKRLIARPYQAALREA
                     ARDKRKVQGLQELYATWTRSLAELTPGLDEIDAGYKQRVTHPYGAFEKQIQALREPTP
                     VAAAKPATKPATKKKSASQ"
     gene            complement(2677..3417)
                     /gene="fabG"
                     /locus_tag="VHT22_07970"
     CDS             complement(2677..3417)
                     /gene="fabG"
                     /locus_tag="VHT22_07970"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017510080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX3137531.1"
                     /translation="MRLKGKVSIITGAGQGIGQATAVKFAQEGAKVAVCDINEATIQE
                     TIAKIEEFGGESTGFVIDVTDKQSIARMVEGVMAKWGRIDTLVNNAGIVQDAQFRKMS
                     DDQFDRVVDVNLKGVYNCTKAVVDIMLEQNSGCILNASSIVGIYGNFGQTNYAATKFG
                     VIGMVKTWARELGRKGIRANAICPGFIHTPILASMPDKVVKMMEDRVPLGRLGQPEEI
                     ANTYAWLASDEASYINGAVIEVSGGATI"
BASE COUNT          620 a         1182 c         1151 g          673 t
ORIGIN      
        1 ctcatcaccc ccgaagccct ggtggcagtt ctggtaacct ttacccgcgg gagttagcct
       61 tctaccgcca ccggcgcgaa aaaagctcgg acatcagcca actcgcgggt gcgccgcatc
      121 ggcggcagcg cctgccagat gcgccggcca tacggcttgt cgaccagccg ccggtcgcag
      181 atcatcagga cgccgcggtc ggtctccgag cggatcaacc tgcccgcgcc ctgcttgagg
      241 ctgatcgccg cttgcggcaa ttgataatcg aagaacggat tgccgccctg gcgcgcgata
      301 tgctcgagcc gcgcagcaag cagcggatcg tcgggcggag cgaacggcag cttgtcgatg
      361 atcaccaccg acaaggcttc gccttgcaca tcgacgccct cccagaagct ctggctgccc
      421 aacaagacgg cgttgcccag gcggcggaaa cgggtaagca attcggtgcg tgaaccctcg
      481 ccttgcacca gcagcggaaa atcgatccct tcgcgtttga agagagcctc cagcgattcg
      541 cgcgcgcggg ccagtgcgcg gagcgtcgtg aacagcaaaa aggcgcgacc gccgctggcc
      601 ttcagcacgg gtagcgcggc cgcgacgacc gcatcggtgt gctcgaagct gttcggttcg
      661 ggcaattcgc gcggcaaata cagcagggcc tgtcgcgcat agtcgaatgg gctctcccag
      721 tagccggtcg cggcctcgcc caagcccagc tcgcgctgat agagcgagaa gtcgctgccg
      781 atcgccaagg tcgccgacgt gaagatccag gctcgggcat ggtcggtgac ctgcttggcg
      841 aatatttcgg ccaccgatag cggcgacgcc cgcagttgcc accccgacgc gctgacgtcg
      901 agccagcgga tccactccgg atcgtcgtga tcgcgccacc gcgcgagccg cgcaagcgca
      961 tcgtcggcgc gatgcgcaca ttgcgccaga tcgtcgctgc gctcggcctg cttgccaagc
     1021 tcgaccgcga ctgccgccag cgccgcgtcc agcgcgtcga gcgcctcatc gaaccccggg
     1081 cgccgcatcg caagtgcgcg cggcagtttg cccggcgttt ctcctgcggc caaccggaac
     1141 cggcgcagcg ccaagccgag cgcctgcgcg gcagcgggca gcggggcgta gtcgcgagcc
     1201 gccttcaacc cttcgagctc ggcgtcgcgc gcaagctcac ccagttgacc gccggtaact
     1261 tcctcgccga aaaacagcgt cgcggtctcg ggcagctggt gcgcctcgtc gagaatcacc
     1321 gtattcgacg cgggcagcag ctcggcgatg ccctcatcct tgagcatgac gtcggcgaag
     1381 aacagatggt ggttgatgac gacgacgtcg gcggcgagcg cctgcttgcg cgcattcatc
     1441 acaaagcact cgttgtaatg tccgcattcg gcgccgagac agttctcgcg cgtcgaggtc
     1501 acgtcggccc agatcgcggc attctcgggc acgtcggcga cctcgctctt gtcgccgctc
     1561 gccgacgtgc gcacgaagga ttcgatccgc ttcacatagc cgatgtcggc gcgcgacgcg
     1621 aacgtcggct gcgccgccgt ccgcgccaga tgatggtggc agacatagtt gccgcggccc
     1681 ttgagcaggg caacggtgat cgggatgcgc agcgcatcgc gcaccagcgg cagatcacgc
     1741 tgatagagct ggtcctgcag cgtcttggtc ccggtcgaaa tgatcacctt gccgccggaa
     1801 agcagggcgg gaaccagata ggcgaaggtc ttgccggtgc cggtcccggc ttcggcgagc
     1861 agctggccgc gcgtcttgat ggcgagcgcg atcgcctcgg ccatcgcgat ttgctgcggc
     1921 cgcggccggt agccgggaac attgcgcgca agcgctccgt cggcgttgaa aaccgaaccg
     1981 agctcggtgt cgctcagcgt tgacctctta gcgcgtcggc ctcgcacgcc gcgtcaccat
     2041 tgcggcggcc tgtcgtcact gcgaagccga ttttttcttg gttgccggct tggttgcagg
     2101 tttggcggcg gcgaccggcg tcggttcgcg cagcgcctgg atctgcttct cgaacgcgcc
     2161 gtacggatgg gtgacgcgct gcttgtagcc ggcatcgatt tcgtcaagcc ccggtgtcag
     2221 ctcggcaagg cttcgggtcc aggtcgcata aagttcctgc agtccctgca ctttcctctt
     2281 gtcgcgcgcg gcttcgcgca atgccgcctg gtaggggcga gcgatcagac gcttgttctt
     2341 ttcgatgcag ccctcggtct tggcgccgtt gacgccttgc tgcgcgccgg ccagcccgaa
     2401 ttccatgatc tcgatgctct tgaccaggtg gcacatcgcg taggtatgcg ggaccgcctc
     2461 gaaaaagcag tcggtgccgc tgccgttgaa agccgtcggg cagatgtatg gattgacgtt
     2521 gtccagggcg tacaccgatg cggccggcaa caccgcgctc acggcaagca gggcaagcaa
     2581 tgctaatcgg agcatgatgg atcgcccagt caaaaggtcg tccatgctac caagcgcggc
     2641 acgcgcgctc aagcgccgcc gcgagcaacg cgccgatcag atcgtggcgc cgcccgagac
     2701 ttcgatgact gcgccgttga tgtagctcgc ctcgtcggag gcgagccagg cgtaggtgtt
     2761 cgcgatttcc tccggctgcc cgaggcggcc gagcggaacg cggtcttcca tcatcttgac
     2821 gaccttgtcc ggcatcgagg ccaggatcgg cgtgtggatg aagcccggac agatcgcatt
     2881 ggcgcgaatg cccttgcggc cgagctctcg cgcccaggtc ttgaccatgc cgataacgcc
     2941 gaatttggtc gccgcgtaat tggtctggcc gaaattgccg tagatgccga cgatcgatga
     3001 cgcattgagg atgcatccgg aattttgctc gagcatgatg tcgacgacag ccttggtgca
     3061 gttgtagaca cccttcaaat tgacgtcgac gacgcgatcg aactggtcat cgctcatttt
     3121 cctgaattgc gcgtcctgca cgatgccggc gttattcacc agcgtgtcga tccggcccca
     3181 tttggccatc accccttcga ccatgcgcgc gatgctttgc ttgtcggtga cgtcgatcac
     3241 gaagccggtc gactcgccgc cgaactcctc gatcttcgca atggtttcct ggatggtcgc
     3301 ctcgttgatg tcgcaaacgg ccaccttggc gccttcctgg gcaaatttga ccgcggtcgc
     3361 ctgcccgatg ccctggccgg cgccagtgat gatcgaaact ttgcctttga gccgcatgat
     3421 cttccctgat cgttatgggc aggagtgccc cgcttgagtg cggcgactct atcccttttc
     3481 attgcattgc acaacctcgg cggcgaccgc gataggacag gcgcggcgca ccagaagcag
     3541 ccataataaa caatggctta gtcaggatct ggcacggggc tacttcgggc agcgatgcgt
     3601 ggcgatcgcg tcattctgat gcattg
//
LOCUS       DATANS010000542         1844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_822480, whole genome shotgun sequence.
ACCESSION   DATANS010000542 DATANS010000000
VERSION     DATANS010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822480"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1698
                     /locus_tag="VHT22_07975"
     CDS             136..1698
                     /locus_tag="VHT22_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX3137532.1"
                     /translation="MAIRPEALPTDTAALIEMVLALDAENEKLRVAMQTLKEMIFGKR
                     SERLAVLVAEQLALDLDDLETGVTPPVAANDDTPAAKPFGRPPRKKARRNIGALPKHL
                     PRCEQVLEPDTTACPCCRGELHKIGADVSEVLDVIPAILRVLRTIRPKYGCRSCTDGV
                     VQAKVLSRLIESGMASTALVTHVVVSKFAWYLPLYRQVQILAGQGVHLDRATLAGWVK
                     RAAWWLKSLYDLQLRTIQASPRVFCDETPMPVLDPGRRRTRICQFWAHAMDDRPWGGP
                     SPPAVAYVFADSRGTDEIAGQLANFSGILQVDGYAAYKALARGHGGTIRLAFCLAHAR
                     RKFVEVYKTTGSPFAHEVIERLQAIYAIEAEIRGSSAEQRLAVRRTRTASLMDALKAR
                     LTEMVGQLFSQSKLTEAINYALNHWDGLTLFLSDGRVEVDSNTVERSMRPIAMGRRNS
                     LFSGSEGGAESWAILASLVNTAKLHELDPQAYLADVLERIVSGQNKSHQLHELLAWNW
                     KAARERTAQDAA"
BASE COUNT          313 a          585 c          620 g          326 t
ORIGIN      
        1 gacgcagctc gcgatgctgc tcgacgggct cgagtggacg cgggtttcgc caaagcctgt
       61 gaagcagccg gcgctggtcg ggtgagaggt gcggattttg ctggagcata ggatgcgcgc
      121 atgtatcgtc tggttatggc gattcgcccc gaagctcttc cgaccgacac tgcagctctg
      181 atcgagatgg tgctcgcgct cgacgccgag aatgagaagc tgcgcgttgc gatgcagacg
      241 ctcaaggaga tgatcttcgg caagcgctcg gagcggcttg cggtcctggt cgccgagcag
      301 ctcgcgctcg acctggacga tctcgagact ggcgtcacgc cgcctgtggc tgccaacgac
      361 gatacgcctg cggcgaagcc gttcggcagg ccgccgcgca agaaggcgcg ccgcaacatc
      421 ggcgcgttgc ccaaacacct gccgcgctgc gagcaggtgc tggagcccga tacgacggcc
      481 tgcccgtgct gccggggtga gcttcacaag atcggcgcgg acgtcagcga ggttctggac
      541 gtgatcccgg cgatcctgcg ggtgctgcgc acgatccgtc ccaaatatgg ctgccgcagc
      601 tgcaccgatg gtgtggttca ggcgaaggtg ctgtcgcgcc tgatcgagag cggcatggcc
      661 tcgacggcgc tcgtgaccca cgtggtggtc tcgaagttcg cctggtatct gccgctgtac
      721 cggcaggtgc agattctggc cggccagggc gtccatctcg accgggcaac gcttgcgggc
      781 tgggtgaagc gtgcggcttg gtggctcaaa agcctctatg atcttcagct gcgcacgatc
      841 caggcctcgc cgcgggtgtt ctgcgacgag acgccgatgc cggtgctcga ccctggacga
      901 cggcgcaccc ggatctgcca gttctgggcg catgcgatgg acgatcgccc gtggggtggg
      961 ccttcgccgc cggcggtcgc ctatgtgttt gccgacagcc gcggcaccga tgagatcgcc
     1021 ggacaattgg caaacttctc cgggatcctg caggtggacg gctatgccgc ctacaaggcg
     1081 ctcgctcgcg gccatggcgg cacgatccgg ctcgctttct gtcttgctca cgcccgacgc
     1141 aaattcgtcg aggtctataa gacgacgggc tcgccgtttg ctcacgaggt gatcgaacgc
     1201 ctgcaggcca tctatgccat cgaggctgag atccgcggca gcagtgcaga gcagcggctg
     1261 gctgtccgcc gcacccggac tgcatcgctg atggacgcgc tcaaggcgcg actgaccgag
     1321 atggtcggcc agctcttctc ccagtcgaag ctcacggagg ccatcaacta tgcgctcaat
     1381 cactgggatg ggctgacgct gttcctcagc gacggccggg tcgaggttga ttccaacacg
     1441 gtcgagcgtt caatgcgccc tattgctatg ggaagacgca actcattgtt cagcggcagt
     1501 gaaggcggcg ccgaaagctg ggcgatcctt gcctcgctcg tgaacacagc aaagctccat
     1561 gaactcgacc cgcaggccta cctggccgac gtactggaac gcatcgtctc cgggcaaaac
     1621 aagagccatc agctgcacga gctgctggcc tggaactgga aggctgcgcg cgagcgcacc
     1681 gcgcaggacg cagcatgagc gcgcatcgcc attcggaatc gccgccgcta gcggcggctg
     1741 cgatgccgct tccggagctc gagcgatggc tgcaggaccg cgccacccgg cgtcccgtcg
     1801 ccaccaacgt gccgatgctg gacggctacg tggctgctat cgtg
//
LOCUS       DATANS010000543         2599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_490591, whole genome shotgun sequence.
ACCESSION   DATANS010000543 DATANS010000000
VERSION     DATANS010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490591"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2554
                     /locus_tag="VHT22_07980"
     CDS             <1..2554
                     /locus_tag="VHT22_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3137533.1"
                     /translation="DVPRDYITIFSGLGGASPQNILVIPVIFEGQVKGVLELASFERF
                     SPTHEAFLDQLAESIGIVINTIEANTRTEDLLTQSQSLAQELQSRQEELQQTNQELED
                     KAGLLAHQNQEVERKNQEVEQARKALEEKAEQLALTSKYKSEFLANMSHELRTPLNSL
                     LILSDQLSRNTESNLSSKQVEFAKTIHASGNDLLTLINDILDLSKIESGTVLVDPTDL
                     MLEELRRYVERTFRHVAEAKNVDFFIRLGPKLPKSMFADSKRLQQVLKNLLSNAFKFT
                     HEGHVTLSIESAKSGWNADNENLNRAPDVISFSVSDTGIGISSEKQQIIFEAFQQADG
                     STNRKYGGTGLGLAISRELSRLLGGEIRLVSAPRVGSTFTLYLPQIYTSPRPRKAAAP
                     RPGADSSVYGKTAIVSPELSAERAEERPAAEYRGNGGDPTQADVEDAAQLAPQLINEV
                     GDDRDSISLGDKTLLIVENDLAFARFLLDAAREQGFKGIVTSQGVAALALASQFHPSA
                     ISLDLFLPDINGWRVLDRIKNDPATRHIPVAIISTDESREQALNAGAYSFVAKPIQSK
                     DVLDSLLASLNQFAQRPEKRLLVATADPEMRDQVVAYLNADDVRIMSVGDRSAAADLV
                     EREGIDCLVLDAKLSLGHGASSIAESEGEPRLDSLPILLYGGEKGAMELDGWRSLARN
                     VVVRDVQSLDRLLDQASLFLHRDPARIPETHRTVLRRLHDSNHVLKDKRVMIVDDDVR
                     NIFALSSLLEDQGMAVVSHDNGRDAIRHLQAQPDIDVVLMDIMMPELDGVDTIREIRK
                     IPACRDLPIIAVTAKAMKGDREKCMEAGAWDYLSKPVDTELMVGVLRAWLSR"
BASE COUNT          505 a          840 c          826 g          428 t
ORIGIN      
        1 cgacgtgccg cgcgactaca tcactatctt ctccggtctc ggaggcgcgt cgccgcagaa
       61 catcctggtg atcccggtta ttttcgaggg ccaggtcaag ggcgtgctcg agctcgcctc
      121 gttcgagcgc ttcagtccga cgcacgaagc ctttctcgac cagctcgcgg aaagcatcgg
      181 catcgtgata aacacgatcg aggccaacac gcgtaccgag gacttgctga cgcaatcgca
      241 gtcgctggcg caggagctgc agagccggca ggaggaactg cagcagacca atcaggagct
      301 cgaggacaag gccggcctgc tggcccacca gaaccaggag gtcgagcgca agaaccagga
      361 agtcgagcag gcgcggaagg cgctggagga aaaggccgag cagctggcgc tcacctcgaa
      421 gtacaagtcc gagttcctcg ccaacatgtc gcacgagctg cgcacgccgc tcaacagcct
      481 gctgatcctg tccgatcagc tgtcacgcaa caccgagagc aatctttcgt cgaagcaggt
      541 cgagttcgcc aagacgatcc acgcctcggg caacgacctc ctgacgctga tcaacgacat
      601 cctcgacctg tcgaagatcg aatccggcac ggtgctggtc gatccgaccg acctgatgct
      661 cgaggagttg cggcgctacg tcgagcgcac cttccgccac gtggccgagg cgaagaatgt
      721 cgatttcttc atccgtctcg gtccgaagct gcccaagtcg atgttcgccg acagcaagcg
      781 cttgcagcag gtgctgaaga acctgctgtc gaacgccttc aagttcactc acgaaggcca
      841 tgtgaccttg agcatcgagt cggccaagag cggctggaat gcggacaacg agaacctcaa
      901 ccgcgcgccc gacgtcatct cgttctcggt cagcgatacc ggcatcggca tctcgtccga
      961 gaagcagcag atcatcttcg aggcattcca gcaggctgac ggcagcacca accgcaagta
     1021 tggcgggacc ggactggggc tcgcgatcag ccgcgagctt tcgcggctgc tcggcggcga
     1081 gatccggctg gtcagcgcac cgcgcgtcgg cagcacgttc accctgtacc tgccgcagat
     1141 ctacacgtcg ccgcgcccgc gcaaggccgc tgcgccgagg ccgggcgccg atagctcggt
     1201 ttacggcaag accgcgatcg tctcgcccga gctttccgcc gagcgggcgg aagaacggcc
     1261 ggccgccgaa taccgcggca acggcggcga tccaacgcag gcggatgtgg aggacgccgc
     1321 gcaattagcg ccccagctga tcaatgaagt cggcgacgac cgcgattcga tcagtctcgg
     1381 cgacaagacg ctgctgatcg tcgagaacga tctggcgttt gcgcgcttcc tgctcgatgc
     1441 ggcgcgcgag cagggcttca agggcatcgt gacctcgcag ggcgtggcgg cgcttgcgct
     1501 ggccagccag ttccatcctt ctgcgatcag tctcgacctc ttcctgcccg atatcaacgg
     1561 ctggcgggtg ctcgaccgga tcaagaacga ccccgcgacg cggcacattc cggtcgctat
     1621 catttcgacc gacgaatccc gcgagcaggc gctcaacgct ggcgcgtatt cgttcgtcgc
     1681 caagccgatc cagagcaagg acgtcctcga ttcgctgctg gcgagcctaa accagttcgc
     1741 gcagcgtccc gagaaacggc tgctggtggc cacggcggat cccgagatgc gcgaccaggt
     1801 cgtcgcctat ctcaatgccg atgacgtgcg catcatgtcg gtcggcgacc gcagcgccgc
     1861 ggccgacctg gtcgagcggg aaggtatcga ctgtctggtg ctcgacgcca agctgtcgtt
     1921 gggacacggc gcttcgtcga tcgcagagag cgaaggcgag ccccggctcg acagcctgcc
     1981 gatcctgctc tatggcggcg aaaagggcgc gatggagctc gacggctggc ggagcctcgc
     2041 ccgcaacgtc gtcgttcgcg acgtgcagtc gctcgatcgt ctgctcgatc aggccagcct
     2101 gttcctgcat cgcgacccgg cgcgaatccc ggaaacgcac cgcacagtgc tgcgccggct
     2161 gcacgactcg aaccacgtcc tgaaggacaa gcgcgtgatg atcgtcgacg acgacgtgcg
     2221 caacatcttc gcgctgtcct cgctgctcga ggatcagggc atggccgtcg tatcgcacga
     2281 caacgggcgc gacgctattc ggcacctgca agcgcagccc gatatcgacg tcgtgctgat
     2341 ggacatcatg atgcccgagc tcgacggcgt ggacaccatc cgggagatac ggaagattcc
     2401 ggcctgtcgg gacctgccga tcatcgcggt caccgcgaag gcgatgaagg gcgaccgtga
     2461 aaagtgcatg gaggcaggtg cgtgggatta tctgtcgaag ccggtcgata ccgagctgat
     2521 ggtcggcgtg ttgcgcgcct ggctgtcgcg ctgaccggaa tgcggctgtc atcgccaggg
     2581 attcggaaaa tcagcgccg
//
LOCUS       DATANS010000544         2045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_448700, whole genome shotgun sequence.
ACCESSION   DATANS010000544 DATANS010000000
VERSION     DATANS010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448700"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VHT22_07985"
     CDS             <1..788
                     /locus_tag="VHT22_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX3137534.1"
                     /translation="WIWRLRPGPDSGLTAKLHARLVDNAVSIGRGDLAPLCSAAREHR
                     VTIVCGMTERDDDLSRGTIYNTAVIIGDDGSLRNKHRKLMPTNPERMVWGFGDGSTLN
                     VVDTPCGRIGTMLCWESYMPLARYALYAQGVEVYIAPTYDSGDGWIGTMQHIAREGCC
                     WVVGSGTLLNARDLPADFPDQARLYPDAGEWINDGDSVVVAPGGKIVAGPLRREAGIL
                     YADIDLEAVAAARRRIDVAGHYARPDVFRLHVNRQRQSPVSFD"
     gene            796..1233
                     /locus_tag="VHT22_07990"
     CDS             796..1233
                     /locus_tag="VHT22_07990"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX3137535.1"
                     /translation="MAARQQRAAVPREIPAAMLTAMKQGAVEAAHRAYAPYSHFAVGA
                     AVLSDDGSIHTGANVENASFGLSICAERNAIFRAVADGARRIEAMVVYTPTADATPPC
                     GACRQVLAEFGADALIVCCSDGAADRRYHLADLLPEAFGPKHL"
     gene            1273..2031
                     /locus_tag="VHT22_07995"
     CDS             1273..2031
                     /locus_tag="VHT22_07995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3137536.1"
                     /translation="MSSSTKNAIVTGAGSGIGRAVALALLNDGFSVALAGRRRDALDQ
                     TVSAAGAAANRALAVPTDVSDPASVRALFAKVKETFGRLDLLFNNAGVGAPGINLEDL
                     SVEQWKTVVDINLTGVFLCMQEAFRMMKSQEPRGGRIINNGSISAHAPRPNSAPYTAT
                     KHAITGLTKSGSLDGRKYDIACGQIDIGNAATELAARMAKGVPQASGQVAVEPLMDVG
                     HVASAVLYMASLPLDANVQFMTIMATKMPFVGRG"
BASE COUNT          363 a          687 c          677 g          318 t
ORIGIN      
        1 cctggatctg gcgcttgcga cccggtcccg acagcggcct caccgcgaaa ctgcacgcgc
       61 ggctggtcga caacgcggta agcatcggcc gaggcgacct cgccccgctg tgcagcgccg
      121 cgcgcgagca tcgggtgacg atcgtctgcg gcatgaccga gcgcgacgac gatttgagcc
      181 gcggcacgat ctacaacacc gccgtcatca tcggcgacga tggcagcctg cgcaacaagc
      241 accgcaagct gatgccgacc aacccggaac gcatggtctg gggtttcggc gacggctcga
      301 cgctcaacgt cgtcgacacg ccctgcggcc gcatcgggac gatgctctgc tgggagagct
      361 acatgccgct cgcgcgctac gcgctgtacg cgcagggcgt cgaagtctac atcgcgccga
      421 cctacgacag cggcgacggg tggatcggaa cgatgcagca catcgcacgc gaaggctgtt
      481 gctgggtcgt cggcagcgga accctgctca atgcgcgtga cctgccggcc gattttccgg
      541 accaggcgcg actctatccg gatgccggcg agtggatcaa cgacggcgat tcggtcgtcg
      601 tcgcgcccgg agggaaaatc gtcgccggac cgttgcgcag ggaagccggc atactgtacg
      661 ccgacatcga cctcgaggcg gtcgctgcgg cgcgccggcg catcgacgtt gccggacatt
      721 acgcgcgccc cgacgtcttt cgcctgcacg tcaaccggca acggcagtcg ccggtcagct
      781 tcgattgacg cgagcatggc tgcccgacaa cagcgcgccg cggtgccgcg cgagattccg
      841 gcggcgatgc tcaccgcaat gaagcaaggc gccgtcgagg ccgcgcatcg cgcctatgcg
      901 ccgtattcgc atttcgcggt cggggcggcg gtgctctccg acgatggcag catccacacc
      961 ggcgccaatg tcgagaacgc gtcgttcgga ttgtcgatat gcgccgagcg caacgcgatt
     1021 tttcgcgccg tggccgacgg cgcgcgaagg atcgaggcga tggtcgtcta tacgcccact
     1081 gccgacgcga cacctccctg cggagcctgc cgccaggtcc tcgccgagtt cggcgccgac
     1141 gcgctgatcg tctgctgcag cgacggcgcc gccgaccgcc gttaccatct cgccgacctg
     1201 ttgccggagg ccttcggccc gaagcatctg taacaccttc gaccatcgac cacgccaata
     1261 ggagacctgc cgatgagctc gtccacgaag aacgcgatcg tcaccggagc gggcagcggc
     1321 atcggccgcg ccgtcgcgct tgccctgttg aacgacggct tttccgtggc gctcgccggc
     1381 cggcgacgcg acgcgctcga tcagacggtg agcgcggcgg gcgccgcggc aaatcgcgca
     1441 ctggccgtgc cgaccgacgt cagcgatccg gcgtcggtgc gggcgctgtt tgccaaggtc
     1501 aaagagacgt tcggccgtct agatctgctg ttcaacaacg ccggcgtcgg cgcccctgga
     1561 atcaacctcg aggacttgag cgtcgagcag tggaagacgg tcgtcgacat caatctgacc
     1621 ggggtattcc tctgtatgca ggaagcgttc cggatgatga aaagccagga gccgcgcggc
     1681 ggacgcatta tcaacaacgg ctcgatttcg gcgcatgcac cgcgaccgaa ctccgccccg
     1741 tacacggcga ccaagcatgc gatcaccggg ctgacgaagt cgggctcgct cgacggaaga
     1801 aagtacgaca tcgcgtgcgg ccagatagac atcggcaatg cggcgaccga gctggcggcg
     1861 cgcatggcca aaggggtgcc gcaggcaagc ggtcaggtcg ccgtcgagcc gctgatggat
     1921 gtcggccacg tagccagcgc cgtgctctat atggcgagcc tgccgctgga tgccaacgtt
     1981 cagttcatga ccatcatggc gaccaaaatg ccttttgtcg ggcgcggcta gcgtcctgcg
     2041 tagac
//
LOCUS       DATANS010000545         2879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_573371, whole genome shotgun sequence.
ACCESSION   DATANS010000545 DATANS010000000
VERSION     DATANS010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573371"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VHT22_08000"
     CDS             <1..404
                     /locus_tag="VHT22_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137537.1"
                     /translation="AIRFEEIKPGDKLGVTSMPGADGMPVAVEVHYLPPGVPEGQGPW
                     DLQPGSTMTNAIVAAMVTATGNRELILQFQGTTQKIAVPENAALARAVPGSRSDLVVG
                     EYVFTVAQGVNDQNLSAARVQVSKDGVKPPL"
     gene            complement(440..751)
                     /locus_tag="VHT22_08005"
     CDS             complement(440..751)
                     /locus_tag="VHT22_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137538.1"
                     /translation="MDPVAPGARGQLRPTVYPGGNHYLGVEDQRAFTTAVARQGYWPD
                     EFLLDDRRLPGKRPAHAPTATLSITVWHLPTGRGATYFGGPGRVWVAEFLVDLMGGTF
                     G"
     gene            1013..1486
                     /locus_tag="VHT22_08010"
     CDS             1013..1486
                     /locus_tag="VHT22_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX3137539.1"
                     /translation="MSLTISSTAFAHNAEIPSLYTCEGKDISPPLAWSGAPAGTKSLA
                     LIVDDPDAPDPAAPKTTWVHWVLYAIPPTADALPEAVASSALPAGTREGVNDWKRTGY
                     GGPCPPIGRHRYFHKLYALDVLPDLQRADKAALEKAMQGHILAQAVLLGTYRKRK"
     gene            1766..2725
                     /locus_tag="VHT22_08015"
     CDS             1766..2725
                     /locus_tag="VHT22_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3137540.1"
                     /translation="MDSVIALADLQSIYSVAEVDRAAEDGAARRNEGLKSWYDRMREL
                     GGSRYIIKPSTAAAVDELHESSPNFTNVVDDLRKYLALAIAGNEAVQFTPLLLLGEPG
                     LGKTYFAKKLAQALGTGFEFVSMSSLTAGWILTGASAQWNNARPGKVAHTLIDGEFAN
                     PVVVLDEIDKAGGDSRYDPMGALYTLLERDTAAHFKDEFIDVDMDASHILWIATANDE
                     SMIPEPILNRMNVYAIDRPDAAGSRTIALAVYHEILDAHRWPFPPEPTESVLERLAAV
                     PARDMRKLLLDAFGTARLANRDYLVPEDIDAKKLCGRKARVGF"
BASE COUNT          544 a          936 c          920 g          479 t
ORIGIN      
        1 aggcgattcg attcgaggaa atcaagcccg gggacaagct cggcgtgaca tcgatgcccg
       61 gcgcggacgg catgccggtg gcggtcgagg tgcattacct tccgcctggc gttccagagg
      121 gacagggtcc ttgggatctt cagccgggat cgacgatgac caatgcgatt gtcgcggcga
      181 tggtcacagc caccggcaat cgcgagctca tcttgcaatt ccagggcacg acgcagaaga
      241 tcgccgtccc cgaaaacgca gcccttgcgc gcgcggttcc cggcagccgc agcgatcttg
      301 tcgtcggtga gtacgtgttc accgtcgccc agggggtgaa cgaccagaac ttgagcgccg
      361 cgcgcgtcca ggtcagcaag gatggcgtga agccgccgtt gtaggcggct tggcgccggg
      421 gcgtgctagt taagacgaac tagccgaacg tcccgcccat cagatcgacc agaaactcgg
      481 cgacccaaac cctgcccgga ccgccgaagt aggtagcgcc gcgcccggtc ggaagatgcc
      541 agacggtgat cgacaacgtt gcggtcggcg catgcgccgg ccgcttgcca ggcaggcgcc
      601 tgtcgtcaag caggaactcg tcgggccaat aaccctgccg cgcgacagct gtcgtgaagg
      661 cgcgctgatc ttccacgccc agataatgat tgccacccgg gtataccgtc ggccgcagct
      721 gcccccttgc cccaggcgcg accggatcca ttagtgcttt gattttggct ctcccttgca
      781 cgcttcgtcg cgcccggacc aagcgcaatg cgcggcccgg accgacgcgc ttgagttagc
      841 aacgtccgcg ccacggcttg cgaatcagca gcttagtatt cgatcacgcc ttcgcgggtg
      901 accgattgta agaaagcgcg gcggtgagca gtttgtagcg cacgttggca ttccgggcaa
      961 tgaactcggg tggccgacga acgatcacat cttcgagaga gaggagacgc cgatgagcct
     1021 gacgatcagt tcgaccgcgt tcgcgcacaa cgccgaaata ccctccctct atacgtgcga
     1081 gggcaaggac atctcgccgc cgctagcctg gagcggcgcg ccggcaggta cgaagagcct
     1141 ggcgctgatc gtcgacgatc ccgacgcgcc cgatccggcc gcccccaaaa cgacctgggt
     1201 gcactgggtg ctctacgcta tcccgcccac tgcggacgcc ctgccggaag ccgtagcatc
     1261 gtcggcgctg ccggccggca cacgcgaggg cgtcaacgac tggaagcgca ccggctacgg
     1321 cggcccctgt ccgccgatcg gccgccatcg ctacttccac aagctgtacg ccctcgacgt
     1381 gctgcccgac ctgcagcgcg ccgacaaggc ggcgctggaa aaagcgatgc agggacacat
     1441 cctcgcccaa gcggttttgc tgggcaccta tcggaagcgc aaatagttgc actttgcggc
     1501 gggtggcgcg cggccggcgc tccttcccgg ggcgctatcg tgcctcatcg gcgtgcgccc
     1561 gcccggcttc gcgcgagtcg gcccatccat ccggcgtcag cgcgcattgc atcgcaacat
     1621 ctattggttg atcccgccgg ctgcttgaga gtagagtccg cggagtggac gaggctcgct
     1681 ggaggatgct gcaaaatcgg gccgcggctg tcattcggtg gcacggttag tgcatgtggc
     1741 ttatcacgga ggctagataa ccaccatgga ttccgtcatc gcgctcgcgg acctgcaatc
     1801 gatctactcg gtcgccgaag tcgaccgggc ggccgaggac ggcgcagccc ggcgcaatga
     1861 aggactgaaa agctggtacg accgcatgcg cgagctcggc ggctcgcggt acatcatcaa
     1921 gccgtcgact gcggccgcgg tcgacgagtt gcatgagtca tcgccgaatt tcaccaacgt
     1981 cgtcgacgat ctgcgcaaat atctggcgct ggccatcgcc ggcaacgaag cggtgcagtt
     2041 cacgccgctg ctgttgctgg gcgagcccgg cctcggcaag acctatttcg cgaaaaagct
     2101 ggcccaggca ctgggcaccg ggttcgagtt cgtctcgatg agctcgctga ctgccggctg
     2161 gatcctgacc ggcgcatcgg cgcagtggaa caacgcgcgg ccgggcaagg tcgcgcacac
     2221 cttgatcgat ggtgaatttg ccaatccggt cgtcgtgctc gacgagatcg acaaggccgg
     2281 cggcgattcg cgttacgacc cgatgggcgc gctgtatacg ctgctcgagc gcgacaccgc
     2341 ggcgcatttc aaggacgagt tcatagacgt cgacatggat gcctcgcaca tcctgtggat
     2401 cgcgaccgcg aacgacgagt cgatgattcc cgagccgatc ctcaatcgaa tgaacgtata
     2461 cgcgatcgac cggcccgatg cagcaggctc ccgcacaatc gcgctggccg tttatcacga
     2521 gattctggac gcgcatcgtt ggccgttccc gcccgagccg acagagtcgg tgctcgagcg
     2581 gctggccgcg gtgccggcgc gcgacatgcg aaagctgctg ctcgacgcat tcggcacggc
     2641 ccggctcgcc aaccgcgatt acctggtacc cgaagacatc gacgcgaaaa agctctgcgg
     2701 ccggaaggct cgcgtaggat tctaaaaacg aaccgcaacg tccggcgcgt tggaaattca
     2761 ggctcggcgg gcgtatccta gtcgcatgac atcgcgccag cgcaaggcgg tcaagctcgt
     2821 ccatccgacg ccgcaacagg aagaagcgtc gttcatccgc agtctggaaa agcacggcc
//
LOCUS       DATANS010000546         1739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_490704, whole genome shotgun sequence.
ACCESSION   DATANS010000546 DATANS010000000
VERSION     DATANS010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490704"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VHT22_08020"
     CDS             complement(<1..786)
                     /locus_tag="VHT22_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3137541.1"
                     /translation="MVVTRRVCLFALLALAAFAAEAQQRFITVASTTSTEQSGLFGYL
                     LPIFETKTGIKVHVVALGTGQALDVARRGDADVVFVHARSAEEKFLAEGEGVKRFPVM
                     YNDFVLIGPKSDPAKIGGGKDIVVALTKLETAHAPFVSRGDRSGTHMAELALWNTAGI
                     DIDKSKGAWYRDVGQGMGPALNTAAAMNAYILADRGTWLAFKNRLDLTILVEGDKRLF
                     NQYGVILVNPVKHPNVKRELGQQFIDWLISPEGQAAIAGYKIGG"
     gene            complement(780..1493)
                     /locus_tag="VHT22_08025"
     CDS             complement(780..1493)
                     /locus_tag="VHT22_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3137542.1"
                     /translation="MSLLPLRLDHVTFTAGGRRIIDDLSLAIDAGPRTVIVGPNGAGK
                     SVLLRLCHGLLAPSSGSIAWNSAEPAGAPRRQAMVFQRPVLLRRSALANVAHALGIAG
                     VPARIRSQRAREALSKVGLERHAAHPARVLSGGEQQRLALARAWALQPEIIFLDEPTA
                     SLDPGATHEVENVILAMVAAGTKIVLVTHNFGQARRMGDEILFLHQGKIAERASAQTF
                     FKHPRSVEAARFLEGELPW"
     gene            complement(1517..>1739)
                     /locus_tag="VHT22_08030"
     CDS             complement(1517..>1739)
                     /locus_tag="VHT22_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014872701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3137543.1"
                     /translation="AAFGRAVSEVGAVMVVGGNIDGFTRVMTTAIALETSKGDLPLAL
                     GLGVILVTIVVALNAAAYLVKETAQRRYG"
BASE COUNT          308 a          596 c          541 g          294 t
ORIGIN      
        1 gccgcctatc ttgtagccgg cgatggcagc ttgcccctcg ggtgagatca gccagtcgat
       61 gaactgctgg ccaagctccc ttttcacgtt cgggtgcttg acggggttca ccagtatcac
      121 gccgtactgg ttgaatagcc gcttgtcgcc ctcgaccagg atcgtcaggt cgagacggtt
      181 cttgaatgcg agccaggtcc cccggtcggc aaggatgtaa gcattcatcg ccgcagcggt
      241 attgagcgct ggccccatgc cctggccgac gtcgcgatac caggctccct tgctcttgtc
      301 gatgtcgatg cccgcggtat tccatagcgc gagctccgcc atatgcgtgc cgctgcgatc
      361 gccgcgcgac acgaacgggg catgggcggt ctccaatttc gtcaatgcga cgacgatgtc
      421 cttgccgccg ccgatttttg ccggatcgct tttcgggccg atcagcacga agtcgttgta
      481 catcactgga aagcgcttca ccccttcgcc ctcggccaga aatttctcct cggcggagcg
      541 cgcgtggacg aagacgacgt cggcgtcgcc gcgccgcgcg acgtcgagcg cctgccctgt
      601 gccgagcgcc acgacgtgca ccttgatgcc cgtcttggtt tcgaagatcg gcaacaggta
      661 gccgaacaga cccgattgct cggtcgacgt ggtcgaggcg accgtaatga atcgctgctg
      721 cgcctcggcc gcaaatgcgg cgagcgccag cagagcgaac aggcaaacgc gacgagtcac
      781 taccatggca actctccttc gaggaaacgt gcagcttcga cagaccgcgg atgcttgaaa
      841 aaggtctggg cgcttgcgcg ctccgcgatc tttccctgat gcagaaagag gatctcgtcg
      901 cccatgcgcc gcgcctgtcc aaagttgtgg gtgaccagca cgatcttggt acccgcggcg
      961 accatcgcca ggatcacgtt ctctacctcg tgggttgcgc ccggatcgag gctcgctgtc
     1021 ggttcgtcga ggaaaatgat ctcaggctgc aatgcccagg cgcgggccag cgcgaggcgc
     1081 tgctgctcgc cgccggacaa aacgcgtgcc ggatgcgcgg cgtgccgctc gaggcccacc
     1141 ttggacagcg cctcgcgcgc acgctgcgag cggatccggg ccggaacgcc cgcgatgccg
     1201 agcgcgtgcg cgacgttggc cagcgccgag cgacgaagca gcaccggccg ctgaaacacc
     1261 atcgcctgac gccgcggcgc cccggccggc tccgccgaat tccaggcgat cgagcccgag
     1321 gacggtgcca gcaagccgtg acacaggcga agcaatacgc tcttgcccgc tccgttgggc
     1381 ccgacgatca ccgtgcgcgg cccggcatcg atcgccaacg acaggtcatc aataatgcgc
     1441 cggccgccag cggtgaacgt cacgtggtcg agacgcaacg gcaggagcga catcggcgac
     1501 cgcatatcca tcaccgtcac ccgtaacgcc gttgcgcggt ttccttgacc aggtacgccg
     1561 ccgcgttcag tgccaccacg atcgtgacca gaatgacccc gagccccagc gcgagcggaa
     1621 gatcgccctt gctcgtctcc agcgcgatcg cggtggtcat cacgcgcgtg aagccgtcga
     1681 tgttgccgcc caccaccatc acggcaccga cctccgagac cgcgcggccg aacgctgcg
//
LOCUS       DATANS010000547         3293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_573541, whole genome shotgun sequence.
ACCESSION   DATANS010000547 DATANS010000000
VERSION     DATANS010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573541"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VHT22_08035"
     CDS             <1..429
                     /locus_tag="VHT22_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX3137544.1"
                     /translation="KDYKPIFSGKHDQSVMGVGSGDYDAGAVASDVFHRMAVRGQIKE
                     ADYRIIYRSQRFPTSSFAYAHDLDPKLVDKMLGCFYDYRFPAEMQKAFDGADRFFPIN
                     YKTTWQVVRDVAKGSGGGFDKATYDKETAREAAEAAKKKN"
     gene            484..1233
                     /gene="phnC"
                     /locus_tag="VHT22_08040"
     CDS             484..1233
                     /gene="phnC"
                     /locus_tag="VHT22_08040"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEX3137545.1"
                     /translation="MLSIKRLSKAYVAGKPVLIDIDLEIASRGITAIIGASGTGKSTL
                     IRCINRLVEPTSGAIEFEGRDLVKLSRASLRQARRHIGMVFQEYNLVERLTVMENLLS
                     GRLGYVSAWRAWRRKFPPADIARAFELLDVVGLAGFANQRADSLSGGQRQRVGIARAV
                     MQQPSLLLADEPTSSLDPKTSVEVMQLLTDIARERGIPVVINMHDVELAKRFSDRIVG
                     MAGGKVVFDGPPGELTDSMLKSIYGGEDWLH"
     gene            1252..2118
                     /gene="phnE"
                     /locus_tag="VHT22_08045"
     CDS             1252..2118
                     /gene="phnE"
                     /locus_tag="VHT22_08045"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014524368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEX3137546.1"
                     /translation="MNTAAARPDPFPVDGWKRAGWLLLAVYVVYAAAQMDVSWDRVVS
                     GLHNARHFFERLFPPNFSRWDLLVKGLGESLQIAVLASALGIALSLPIGLAASRNLVP
                     AWMSWPARLLIVAARSFHPVIVAILFVKAVGFGALAGILALALASIGFTAKLFAEAIE
                     EISLKQVEAVRATGAPFGSVIGIGVLPQVFARFIGFATYQLDSNLRNSTMVGIVGAGG
                     IGGTLFAAFQRFDYDFVCAILISIIALIMLGELLATGVRAIFIDGASIGDLFRRGPRA
                     PGGRHERLLEDD"
     gene            2124..2936
                     /gene="phnE"
                     /locus_tag="VHT22_08050"
     CDS             2124..2936
                     /gene="phnE"
                     /locus_tag="VHT22_08050"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEX3137547.1"
                     /translation="MAELAHDRIWRRFTPAQKLARFAVYLVIVAAIVASIRTVEVIPE
                     FLADAPEQVVDLLTRMWPPDTAYYPKGVHAALIETIHIASLGTLLSLLLALPVGLFAA
                     HNICPYLPLNLAAKLILVSSRSVNSLVWALLFVGVFGPGALAGTLAIAFRSIGFVGKL
                     VAEALEDSQPGPIEALSAAGAPGASLLSFGYWPQIKPAFWSIALFRWDINVRESAVLG
                     LVGAGGIGMALDSALNLFQWNRVAVILLAIFAVVIAAEIVVTVARKRMIQPQ"
     gene            2933..>3293
                     /locus_tag="VHT22_08055"
     CDS             2933..>3293
                     /locus_tag="VHT22_08055"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3137548.1"
                     /translation="MTIIAVFNQKGGVGKTTTALNLLAGLAQRKQRPLAIDLDPQAHL
                     SGIFGVRPRLADDSVYSFFVRQRALADVTEIAASGVMVCPGHLDLSKLDTMLGKGVNV
                     VTRLRTALHAPGVVPGPV"
BASE COUNT          500 a         1101 c         1075 g          617 t
ORIGIN      
        1 aaggactaca agccgatctt ctccggcaag cacgaccagt cggtcatggg cgtaggttcg
       61 ggcgactacg acgcgggcgc ggtcgcgtcg gacgtgttcc atcgcatggc ggtacgcggc
      121 caaatcaagg aagccgatta ccgcatcatc tatcgcagcc agagattccc gacgtcgtcg
      181 ttcgcgtacg cgcacgacct cgatccgaag ctggtcgaca agatgctcgg ctgcttctac
      241 gactaccggt tccccgccga gatgcagaag gcgttcgacg gcgccgaccg cttcttcccg
      301 atcaactaca agacgacctg gcaggtcgtg cgcgacgtcg ccaagggctc gggcggaggg
      361 ttcgacaagg cgacctacga caaggagacc gcgcgcgaag cggcggaagc ggcgaaaaag
      421 aagaattgac gcggaaaggc ctcggacgat tccggcccgg gccgaccgcg gacaccgcct
      481 ccagtgctct cgatcaaacg tctgtccaaa gcctatgtcg ccggcaagcc ggttctgatc
      541 gacatcgatc ttgagatcgc ttcgcgcggc attacggcaa tcatcggcgc gtcgggcacc
      601 ggcaagagca cgctgatccg ctgcatcaac cggctggtcg agccgacctc cggcgcaatc
      661 gagttcgagg gccgcgacct ggtcaagctg tcgcgcgctt cgctgcggca ggcgcgccgt
      721 cacatcggca tggtgtttca ggaatacaac ctggtcgagc ggcttacggt gatggagaac
      781 ctgctgtccg ggcggttggg ctacgtgtcg gcgtggcggg cgtggcggcg caaatttccg
      841 ccggctgaca tcgcgcgcgc attcgaactg ctcgacgtcg tggggctggc cggcttcgcc
      901 aatcaacgcg ccgattcgct ctccggcgga caacggcagc gcgtcggcat cgcgcgcgcg
      961 gtgatgcagc agccgtcgct gctgcttgcg gacgagccga cctcgtcgct cgatccgaaa
     1021 acctcggtcg aggtgatgca gctgttgacc gatatcgcgc gcgaacgcgg catcccggtc
     1081 gtcatcaaca tgcacgacgt cgagctggcg aagcgatttt ccgaccgcat cgtcggcatg
     1141 gccggcggca aggtggtttt cgacggcccg ccgggcgagc tcaccgacag catgctgaaa
     1201 tcgatctatg gcggcgaaga ctggctgcac tgaaccgcgg ccgcgctcgc gatgaacact
     1261 gcggccgcgc ggcctgatcc gttcccggtc gacggctgga aacgcgccgg ctggctgctg
     1321 ctcgccgttt atgtcgtcta cgcggccgcg cagatggatg tgtcgtggga ccgcgtcgtg
     1381 tccgggctgc acaatgccag gcactttttc gagcggctgt tcccgccgaa cttcagccgc
     1441 tgggatcttc tggtcaaagg ccttggcgag agcctgcaga tcgcggtcct cgcctcggcc
     1501 ctgggcatcg cgctgtcact gccgatcggg ctcgccgcct cgcgcaacct ggtaccggcc
     1561 tggatgagct ggcccgcgcg actgttgatc gtcgccgcgc ggtcgttcca cccggtcatt
     1621 gtcgcgatcc tgttcgtcaa ggcggtcgga ttcggcgcgc tggccggcat cctcgcgctc
     1681 gcgctcgcct cgatcggctt caccgccaag ctgttcgccg aagcgatcga ggaaatctcg
     1741 ctgaagcagg tcgaggcggt gcgcgccacc ggcgcgccct tcggcagcgt gatcggcatc
     1801 ggcgtgctgc cgcaggtgtt cgcccgcttc atcgggttcg ccacctacca actcgattcc
     1861 aacctgcgta actcgacgat ggtcggcatc gtcggagcgg gtggaatcgg gggaacgctg
     1921 ttcgccgcat tccagcgctt cgactatgac tttgtctgcg ctatcctgat ctcgatcatc
     1981 gcgctgatca tgctgggtga gctgcttgcc accggcgtgc gcgcgatatt catcgacggc
     2041 gcttccatcg gcgatctgtt ccgccgcggc ccccgcgcgc cgggcgggcg ccacgagcgc
     2101 ctcctcgagg acgactgaag ggcatggccg agctcgccca cgatcgcatc tggcgacggt
     2161 tcacgccggc gcagaagctt gcccgtttcg ccgtctacct cgtcatcgtc gcggccatcg
     2221 tcgcatcgat ccgtaccgtc gaggtgatcc cggagttcct cgctgacgcg ccggagcagg
     2281 tagtcgacct gctgacccgc atgtggccgc cggacaccgc ctattatccg aagggcgtcc
     2341 atgccgcgct gatcgagacg atccatatcg cctccctcgg cactctgctc tcgctgctgc
     2401 tggcgttgcc ggtgggcctg ttcgccgcac acaacatctg tccgtacctg ccattgaatc
     2461 tcgccgccaa gctgatcctg gtgtcgtcgc gctcggtcaa ctcgctggtg tgggcgctgc
     2521 tctttgtcgg cgtcttcggt cccggtgcgc tggccggcac cctggcgatc gcgtttcgct
     2581 cgatcggctt cgtcggcaag ctggtggccg aggcgctcga ggacagtcaa cccggtccga
     2641 tcgaggccct ctccgcggcc ggggcgccgg gggcctcgct gctgtctttc ggctactggc
     2701 cgcagatcaa gcccgcgttc tggtcgatcg cgctgtttcg ctgggacatc aacgtgcgcg
     2761 aatccgcggt gctgggcctg gtcggcgccg gcggcatcgg catggcgctc gattcggcgc
     2821 tcaacctgtt ccagtggaac cgggtggcgg tcatcctgct ggccattttt gcggtggtga
     2881 tcgcggcgga gatcgtcgtc accgtggcgc gcaagcgcat gatccagccg caatgaccat
     2941 catcgcggta ttcaaccaga agggcggtgt cggcaagacg acgactgcgc tcaaccttct
     3001 ggccggcctc gcgcagcgca aacagcggcc gcttgccatc gacctcgatc cgcaggcgca
     3061 cctttccggg atattcggcg ttcggccgcg actggccgac gattcggtgt attcgttttt
     3121 cgtgcggcaa cgcgcgcttg ccgacgtcac cgagatcgcg gccagcggcg tcatggtatg
     3181 tccgggccat ctcgacctgt ccaagctcga cacgatgctg ggcaagggcg tcaacgtggt
     3241 gacgcgcctg cggaccgcgc tgcatgcacc gggcgtcgtc cccggccccg tcg
//
LOCUS       DATANS010000548         2159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_153940, whole genome shotgun sequence.
ACCESSION   DATANS010000548 DATANS010000000
VERSION     DATANS010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153940"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="VHT22_08060"
     CDS             complement(<1..1357)
                     /locus_tag="VHT22_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX3137549.1"
                     /translation="MTDRSRDDHTDALRRRLLQAGAGAAALGALPGASLLGAQAQGTF
                     DWKRFKGEKIEVLLVKSPRGDLLSKYHKEFQDMTGIEVGSEMVPEQQQRQKAVIEFNS
                     GNPSFDVIALSYHVQKRQFAKNNWLTDLRPMINDRLMADPNLDFADFAQGGLNYAVEA
                     DGRVMSLPLNLDPWVVYYNKELFAAKGVAFPKTFAQMIDAAAKLNDTGKGVSGFVGRG
                     LKNANVPVWTSFLLGYGGGFVDKSGKLMTDSPEAVESAKMYQTLLAKYGPQGVAGYNW
                     NEAQSLFLQGKAAMWLDGIGFAQPLEDPTKSRIVGKVGYGVMPAGPKQQVSALFADGE
                     GISTFSKKKGPAWFYLQWASNKANQTRMLQAAAGAPVRTSAYAAAQASSDFKSPKEWV
                     ECMLTSAKIAQPGLPIIVPVTEFRDTFGIALTNMINGADAATELKKATAEFQPVLDKS
                     EK"
     gene            complement(1481..2152)
                     /locus_tag="VHT22_08065"
     CDS             complement(1481..2152)
                     /locus_tag="VHT22_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX3137550.1"
                     /translation="MAAFDTPTICNALEVVLPATRARGYTTRPAHCGFPAMRPIVGYA
                     RTARIRARAAPTLPAGKLLELRHGYYRYIDAGRKPSLVLIEDLDGPDAGYGAFWGEVQ
                     SAIHRGLGALGLVTNGSVRDIDQWAPGFQFLAGSIGPSHAYAHVVEFGGEIEVLGMRV
                     RSGDIVHADRHGAVVVPAAAVAGLPAAARQVAEREARILEVARAPGSSAERLIEVFSR
                     LDAIH"
BASE COUNT          355 a          732 c          670 g          402 t
ORIGIN      
        1 ccttctcgct cttgtccaga accggctgga actccgccgt cgccttcttg agctcggtcg
       61 cggcgtccgc gccgttgatc atgttggtga gcgcgatacc gaacgtgtcc cggaactcgg
      121 tgaccggcac gatgatcggc aggcccggct gcgcgatctt agccgatgtc agcatgcatt
      181 cgacccactc cttgggcgac ttgaaatcgc tcgacgcctg cgcggccgca taggcagatg
      241 tgcgcaccgg cgctccggcg gcggcctgca gcatgcgcgt ctggttggcc ttgttcgacg
      301 cccattgcag atagaaccag gccggaccct ttttcttgct gaaggtcgaa atcccctcgc
      361 catcggcgaa cagcgccgag acctgctgct tcggacccgc cggcatcacg ccgtaaccga
      421 ccttgccgac gatgcgcgac ttggtcggat cctcgagcgg ttgcgcgaag ccgatgccgt
      481 cgagccacat cgccgccttg ccctggagaa acaggctttg tgcctcgttc cagttgtagc
      541 ccgcgactcc ctgcgggccg tacttggcga gcagcgtctg gtacatcttc gccgattcga
      601 ccgcttcggg gctgtcggtc atcagcttgc cgctcttgtc gacgaagcct ccgccgtagc
      661 cgagcaggaa gctcgtccat accggcacgt tggcgttctt caggccgcgg ccgacaaacc
      721 cggacacgcc cttgccggta tcgttgagtt tggcggcggc gtcgatcatt tgggcaaacg
      781 tcttcgggaa cgcgacgccc ttggcggcga acagctcctt gttgtaatag acgacccagg
      841 ggtcgaggtt gagcggcagg ctcatcacgc gcccgtcggc ctcgaccgcg taattgagcc
      901 ccccctgcgc gaaatcggcg aaatcgaggt tcggatcggc catcaggcga tcattgatca
      961 tcggccgcag atcggtcagc cagttgtttt tggcgaactg gcgcttttgc acatgatacg
     1021 aaagcgcgat cacgtcgaag ctaggattgc cggaattgaa ttcgatgaca gccttctgcc
     1081 gctgctgctg ctcggggacc atttcagagc cgacctcgat gccggtcatg tcctggaatt
     1141 ccttgtggta cttggagagc agatcgccgc gcgggctctt gaccagcagg acttcgatct
     1201 tttcgccctt gaatcgcttc cagtcgaacg ttccctgggc ctgcgcgccg agcagcgagg
     1261 cgccgggtaa cgcgcccagc gccgcggcgc cggcaccggc ttgcaaaagg cggcgccgca
     1321 aggcgtcggt gtgatcatcg cggctacggt cggtcatcgt aattctcctc cggtggcgtt
     1381 atggcacaac atgatcggag cgttcgcggc gccaccacct gcgcgccgcg cgctttgccc
     1441 tcaaatcata cccgacggca tggcgcctcg tgtcgtgtca ctagtggatg gcgtcgagcc
     1501 gcgaaaacac ttcgatcaat cgctcggcgc tgctgcccgg cgctcgcgct acctccagga
     1561 tacgggcctc gcgttccgcg acttgccgcg ccgccgcggg caggccggcg accgctgccg
     1621 cgggcacgac cactgcgccg tgacggtcgg catgaacgat atcgcccgac cgaacccgca
     1681 tgcccagcac ctcgatttcg ccgccgaact cgaccacatg cgcataggcg tgcgacggtc
     1741 cgatgctacc ggccagaaac tggaatcccg gagcccactg gtcgatgtcg cgcaccgagc
     1801 cattggtcac gagccccagt gcgcccaacc cgcgatgaat cgccgattgc acttcgcccc
     1861 agaacgcgcc gtagcctgcg tcgggaccgt cgagatcctc gatcagcacc agcgacggct
     1921 tgcgcccagc gtcgatgtag cgatagtagc cgtggcgaag ttccagcaac ttcccggcgg
     1981 gtagagtcgg cgcggctctc gcccggattc tggccgtgcg ggcgtatccg acaatcggcc
     2041 gcatcgccgg aaacccgcaa tgcgccgggc gcgtcgtgta gccacgtgcc cgtgtcgccg
     2101 gcagcaccac ctcgagcgcg ttgcaaatgg tgggcgtatc gaacgccgcc agcgcggcg
//
LOCUS       DATANS010000549         1508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_364309, whole genome shotgun sequence.
ACCESSION   DATANS010000549 DATANS010000000
VERSION     DATANS010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364309"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VHT22_08070"
     CDS             complement(<1..981)
                     /locus_tag="VHT22_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX3137551.1"
                     /translation="MTGILLSRGFVATYGKLLDESAQRAGLTLELVHLPDDPQSRLAP
                     ADPARIEVTMLTRDIRFSDHYKSYGDTLLAAANLKFAHFDSTAIEQHPFVAPLARRGV
                     KLTTATGSNGVPVAQTAITTLLMMSRGLPHFIDAQRRKSWEPVRASAQPPDLQGQTII
                     IVGMGTIGTTVARFAQTLGMHVIGIRRKPRSDDDPVDEMHTLAELPKLLPRCDWLVLA
                     CPLVPETRGLVNAQSLALMKKSAGLINVARGALCDEDALIAALRSGQIRCAHLDVYAS
                     EPLPAGSPLWEMPNVIMTPHSAGASAGNELRSVRIFLDNLERWARGEPLLN"
     gene            complement(1028..>1508)
                     /locus_tag="VHT22_08075"
     CDS             complement(1028..>1508)
                     /locus_tag="VHT22_08075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3137552.1"
                     /translation="TIALNLLHPFLTCRHAIPHMMKAAGGSIINVTSHLGLMGSIKPA
                     YAATKGGIISFTKTLAAQYADHGIRANGIAPGSVRTERQIKRYENKEWMLAEKLSPAA
                     RAREIAKKLYPFASGDPGDIAAIAVFLASDESRMLTGTTIAADGGRSSYLKLYTGDE"
BASE COUNT          251 a          465 c          510 g          282 t
ORIGIN      
        1 attgagcagc ggctcgccgc gcgcccagcg ctctagattg tccagaaaga tgcgcacgct
       61 gcgcaattcg ttgccggccg acgcgcccgc gctgtgcggc gtcatgatca cgttcggcat
      121 ctcccacaac ggagagccag cgggcagcgg ctcgctcgcg tagacgtcga gatgcgcgca
      181 gcggatctgc ccgctgcgca gcgcggcgat cagcgcgtct tcatcgcaca acgcaccacg
      241 tgcgacatta atgaggcctg ccgacttttt catcaacgcg agcgattgcg cattgacgag
      301 accgcgcgtt tcgggcacca gcgggcacgc gagcacgagc cagtcgcagc gcggcaatag
      361 tttgggcaat tccgcgagcg tatgcatttc gtcgaccggg tcgtcatcgc tgcgcggttt
      421 gcgccggatc ccgatcacgt gcataccgag cgtttgcgcg aaacgcgcga ctgtcgtgcc
      481 tatcgtgccc atgccgacga tgatgatcgt ttgtccttgc aggtcaggcg gctgcgccga
      541 cgcgcgcacc ggctcccagc ttttgcgccg ctgcgcgtcg atgaagtgcg gcaacccgcg
      601 gctcatcatg agcaacgtcg tgatcgcggt ctgcgcgacg ggcacgccat tgctgccggt
      661 cgcggtcgtc agcttgacgc cgcgcctggc gagtggcgcg acgaaaggat gctgctcgat
      721 cgccgtgctg tcgaaatgcg cgaacttgag attggccgcc gcgagcagcg tgtcgccgta
      781 agacttgtaa tggtcggaaa aacggatgtc gcgcgtgagc atcgtcactt cgatgcgcgc
      841 ggggtccgcc ggcgcaaggc gtgactgcgg atcgtcgggc agatgcacga gctcgagcgt
      901 aaggcccgcg cgctgcgcag actcgtcgag cagcttgccg taggtagcga caaatcctct
      961 ggacagcaat ataccggtca tcgatggttc tctgtgcgac cggtctagcc gcgcaacgac
     1021 gcgcgcatca ttcgtcgccg gtgtaaagct tgagatagga cgagcgtccg ccgtcggcgg
     1081 cgatggtcgt gccggtcagc atgcgcgatt cgtcggacgc cagaaacacc gcgatggcgg
     1141 cgatgtcgcc gggatcgccg gacgcgaacg gatagagttt tttggcgatc tcgcgcgcgc
     1201 gggcggccgg cgagagtttt tcagccagca tccattcctt gttctcgtag cgcttgatct
     1261 ggcgctcggt gcgcaccgag ccgggcgcga tcccgttggc tcggatgccg tggtccgcgt
     1321 actgcgcggc gagcgtcttg gtgaatgaga tgatgccgcc cttggtcgcc gcgtatgcgg
     1381 gcttgatcga acccatcagc cccaggtgcg atgtcacgtt gatgatcgac ccgccggccg
     1441 ccttcatcat gtgcgggatc gcgtgacgac aggtcaggaa cggatgcagg aggtttagcg
     1501 cgatggtg
//
LOCUS       DATANS010000550         5461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_701374, whole genome shotgun sequence.
ACCESSION   DATANS010000550 DATANS010000000
VERSION     DATANS010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701374"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..2488)
                     /locus_tag="VHT22_08080"
                     /pseudo
     CDS             complement(1..2488)
                     /locus_tag="VHT22_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646450.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
     gene            2890..3819
                     /locus_tag="VHT22_08085"
     CDS             2890..3819
                     /locus_tag="VHT22_08085"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3137553.1"
                     /translation="MLDPDIQRLLDTVFNVPVGAEAPDIAQLRAAAETAPVLLGGEPE
                     PVASITDAFAPGENDPVPVRIYRPAAAELLPLLVYAHGGGWVTGSLDSHDRLCRILAN
                     RLRVVLVAVAYRCAPEYVYPAALDDLEAAWRWARAEAKTLGADGMRFAVGGDSSGGGL
                     AAALTLRLRSDGAAQPNLLLLLYPALDATCSRPSYREFATGYSLSAGLMAWYWDAYRA
                     GAAKSAVGLSPLAAADLSGLPPAVIAVAEFDVLRDDALDYAARLEAAGIPVRMIRCTG
                     MIHGFLRWTGAVPAAHRWIDAITEAGRDSLGGG"
     gene            3877..4629
                     /locus_tag="VHT22_08090"
     CDS             3877..4629
                     /locus_tag="VHT22_08090"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3137554.1"
                     /translation="MPAPSDGALRAAFETAPSLAAHCVAAAWYVSRLPRDAGTLLDAM
                     TGSGHLLVPLLEAGFNVHGADSSETALRRCSARLEAAGRSTQLFRQSITALNLPFRYG
                     AAFVSGESFQSLTERVVALDALLRIRAHLIDPGLLLLDLFVPDEADHPPGAPIVELSM
                     LALADGSQIARRSETVIDVARRRSIVHSRFEQRDRTGVLAREDDVREITWYAEDEIAT
                     LVDDAGYHDIRFEVLPWGGGARARHYAVSART"
     gene            complement(4635..>5461)
                     /locus_tag="VHT22_08095"
     CDS             complement(4635..>5461)
                     /locus_tag="VHT22_08095"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX3137555.1"
                     /translation="TMVSKGGVLASAYYGDLGLRPMNDFDVLVPRTQARDAVRVLLGA
                     GWASTQPMAEMLPDAYHSACFQSPDRLDFDLHWHLLPESCFAGAEDPAWQAAEPYTVD
                     GVATQAPCATDLLVIVCAHAAHWSPVSPARWVADALTILRRAPERIDWTRTAMLAQRW
                     HVAPHLADTLSYLAQRWSAGIPPSLLSELRHIPVGRVDRRAYALLGRMPGRLTYLGRP
                     WFRYRLRTRNSSGVAALPGFTEYLKITLGLADDRALTGEIFRRYRRWRADRARGKR"
BASE COUNT          865 a         1806 c         1803 g          987 t
ORIGIN      
        1 gcgccggcac catgacgccc agatcgatga catcgaaatt gttgcactgg aggacgacgc
       61 cgacgatgtt cttgccgatg tcgtgaacgt cgcccttgac cgtcgccatc acgatcgttc
      121 ccttcggctt gcccgcgtcc cccgacctcg ccttctccgc ctcgatgaac gggatcagat
      181 gggcgaccgc ctgcttcatc acgcgcgcgc ttttcacgac ctgcggcagg aacatcttgc
      241 cggcgccgaa caggtcgccg acgacgttca tgccggccat cagcggccct tcgatcacct
      301 gaatcggctt gccgccctgc gcctcgatct tgcgtcgcgc ctcctcggta tcggcgacga
      361 tgtaagtggt gatgcccttg accagcgcat gcgacaggcg cgcctcgacg tcggtgtcgc
      421 gccacgccag gtcggcgacg acatgcttgc cggcggtctt gtacgtctcg gcgaacgtga
      481 ccagccgctc ggtcgggctt tcgccgtcgg cgcgtttgcg attgagcacc acgtcctcga
      541 cgcgctcgcg gagctccggg tcgatgtcgt cgtacacgcc gagctgtccg gcattgacga
      601 tgcccatcgt catgcccgcc ttgatcgcgt gatagaggaa aaccgtgtgg atcgcctcgc
      661 gcaccgggtc gttaccgcgg aacgaaaagc tgacgttgga tacgccgccc gataccttgg
      721 cgtgcggcag atgctgcttg atccagcgcg tcgcattgat gaagtcgacc gcgtagttgt
      781 tgtgctcctc gatgccggtc gcaatggcga agatgttcgg gtcgaagact atgtcttccg
      841 ggggaaaatc ggcttgccgc gtcaacaact cgtaggcccg gcggcagatg tcgaccttgc
      901 gctcgaaggt gtcggcctgg cctttttcgt cgaaggccat gacgatcgcc gcggcgccgt
      961 agcgccgcac cagtcgcgcc tggcgcagga actcggcctc gccctccttc atcgaaatcg
     1021 agttgacgac gggcttgcct tgcacgcatt tgagtccggc ctcgatcacc gtccacttcg
     1081 acgagtcgat catcaccggc acccgcgcga tatcgggctc gccggcgata agattcagga
     1141 acctgaccat cgccgccttg gagtcgagca tcgcttcgtc catgttcacg tcgatcatct
     1201 gggcgccgtt ctggacctgc tggcgggcga cttcgaccgc acccgcgtag tcgccggcga
     1261 gaatcagctt ggcgaacgcg cgcgagccgg tgacgttggt gcgctcacca acgttgacga
     1321 acagcgcatc gtcgccgatc gtcagcggct cgaggccggc caggcgcatc gcgcggggca
     1381 tgggaagttt ggctggtctt cttgttcgtc tccccgccgt cggggagcat cggtttcaca
     1441 ccgccgcgtc ggcggtttcc tcgacgggaa cgatgcgcgg tggcagattc ttcactgctt
     1501 cggcgatcgc gcggatgtgt gccggcgtgg tgccgcagca gccgccgacg atgttgacaa
     1561 agccgttagc cgcgaaatcg gcaacgagcg ccgccgtcgt ctccggcgct tcgtcatagc
     1621 cggtatcgct catcgggttg ggcaggccgg cgttcggata gcacgaaatg aacgtgtccg
     1681 ccacgcgcga gagctcctcg atgtaaggac gcatcagcgc ggcgcccagc gcgcagttga
     1741 ggccgaccgc gagcggacgc gcgtgccgca ccgaattcca gaacgcctcg gtcgtctggc
     1801 cggacagcgt tcgccccgat gcatcggtga tcgttcccga cacgatgatg ggcaggcgga
     1861 ccccgacgcg ctcgaatgac tggtcgatcg cgaaaagcgc ggccttggca ttgagggtgt
     1921 cgaaaacggt ctcgaccagc agcaggtcgg cgccaccgtc gaccaggccg tcgatcgctt
     1981 ccccgtaagc ggcgaccagc tcgtcgaagc tgacgttgcg aaagccggga tcgttcacgt
     2041 cgggcgagat cgtggccgtc cggttggtcg gacccagcgc gccggcgacg aatctcggct
     2101 tgtggggcgt tttcgcggtc caggcgtcgg cacgttcgcg cgcaatcctg gccgccgcga
     2161 aattgatgtc gcgcactttc gcctccagcc cgtagtcgga ctgcgcgatg cgcgtcgcgt
     2221 tgaaagtgtt ggtttcgatg atgtcggcgc cggcttcgag aaacgcgtcg tggatcgccg
     2281 cgacgacggc gggctgggtc aggaccagca ggtcgttgtc gcccttcaga tcgcgcgtat
     2341 ggtcgcagag tccgcaggcc ggcgcgccgc gaaagtcggc ctcgccgagc ttttcagcct
     2401 ggatcatagt gcccatcgcg ccgtcgagga tgaggatgcg ttcccgcagc aggcgctgca
     2461 acagttcggt cttgtcggtc gtcgtcatag gtgtgctgag tcagaaattc gctcgatagt
     2521 tcgcggcggc ttatttggtt ggcaaatgag ccgccatgct gcgttgctca gcctcagcgt
     2581 agcgcgagct acggtttcgg cctcgcgcct tgcctggcgg ccaaatccat ccgctcctct
     2641 atcgtcggaa cttctgactc agcacaccag ctgccagaaa acaaaaaacc cgttcagctt
     2701 gccgccgagc gggtttcagg cgaaagccgc tttagctgga tttgtatagc gcccgcaagc
     2761 tgcggggtca cccccgaatc aaatcggcgc tggctttaag gtaagggttg gccgcaccgt
     2821 tgtcaactgc gtatcaaccg tacgcagcga ttcgggtaat atcttcgcac tgggtcaccg
     2881 atggcgacga tgctcgaccc cgatatccag cgtctgctcg acacggtctt caacgtcccg
     2941 gtcggcgccg aagctcccga catcgcgcag ctgcgtgcgg ctgcggagac cgcgcctgtg
     3001 ctcctgggcg gcgagcccga gccggtggcc tcgattaccg atgcatttgc tcccggcgaa
     3061 aacgacccgg tgccggtgcg catctaccgg cccgccgcag cggagctgct gccgcttctc
     3121 gtctacgcgc acggcggtgg gtgggtaacc ggctcgctgg actcccacga ccggctctgt
     3181 cgaatcctcg ccaatcgatt gcgcgtcgtg ctggtcgccg tcgcttaccg ctgcgcaccg
     3241 gagtacgtct atccggcggc gctggacgat ttggaagccg cctggcgttg ggcccgcgcc
     3301 gaagcgaaga cgctcggcgc cgatggcatg cgcttcgccg tcggaggcga cagctcgggc
     3361 ggtggcctcg ccgcggcgct gacgttgcgc ttgcgctccg acggcgccgc tcagccgaac
     3421 ctgctgctgt tgctctatcc tgcgctggat gcgacctgct cgcggccgtc gtatcgcgaa
     3481 ttcgccaccg gctacagcct ttcggcaggg ctcatggctt ggtactggga tgcttatcgc
     3541 gccggcgcgg cgaaaagcgc cgtcggactg tcgccgttgg cggccgccga cctgtcgggg
     3601 ttgccgccgg cagtgatcgc cgtcgccgaa ttcgatgtat tgcgcgacga cgcgctcgac
     3661 tacgcggcga ggctcgaagc cgcaggcatc ccggttcgca tgatccgttg caccggcatg
     3721 atccacggct ttctacgctg gaccggcgcg gtgccggccg cccatcgctg gatcgacgcg
     3781 atcaccgagg ctggacgcga ctcgctcggg ggaggctaaa gccgattgcg acagcagcag
     3841 ccacgccgaa atctccgcca acgcggcgcg ccggctgtgc cggcgccgag cgacggcgcg
     3901 ctccgcgcgg cgttcgaaac ggcgccatcg ctggccgcgc actgtgtcgc agccgcgtgg
     3961 tacgtgtcgc gcctgccgcg cgacgccggc acgctgctcg acgcgatgac cggcagcgga
     4021 catctgctgg tcccgctgct cgaagcggga ttcaatgtgc acggtgccga ctcgtcggaa
     4081 acggcgctca ggcgttgcag cgcgcggctc gaggcggccg gccgaagcac gcaattgttc
     4141 cggcagagca tcactgcgct caatctgcca ttccggtacg gcgccgcgtt cgtctccggt
     4201 gagtcgttcc agtcgctcac cgagcgcgtc gtggcactgg acgcgctgct gcgcatccgc
     4261 gcgcacctga tcgatccggg cctgttgctg ctggatctgt tcgtacctga cgaagccgac
     4321 cacccgccgg gcgcgcccat cgtagagctc tcgatgctgg cgctggccga cgggtcgcag
     4381 atcgcccgtc gctccgagac cgtgatcgac gtggcgcgcc ggcgcagcat cgtccacagc
     4441 cgtttcgagc agcgtgatcg taccggcgtc ctcgcccgcg aggacgatgt acgcgaaata
     4501 acatggtacg cggaggatga gatcgccacg cttgtcgacg acgccggata tcacgacatc
     4561 cgcttcgagg tgctgccttg gggcggcggt gcgcgagcgc gacactacgc agtatcggca
     4621 agaacttaac ggcgttaccg cttgcctcgg gcgcggtcgg cgcgccagcg ccggtaccgg
     4681 cgaaagatct ctccggtcag tgcgcggtcg tcagcgaggc cgagcgtgat cttcaggtac
     4741 tcggtgaagc ctggcaacgc ggccaccccg gagctatttc gcgttcgcaa cctgtagcgg
     4801 aaccagggtc ggccgagata tgtcagccgg cccggcatgc ggccaagcag ggcgtaggcg
     4861 cggcgatcga cacgaccgac cggaatgtgc cggagctcgc tcagcagcga tggcgggatg
     4921 ccggcactcc atcgttgcgc gaggtaggaa agcgtgtcgg ccaggtgcgg ggcgacatgc
     4981 cagcgttgcg ccagcatcgc ggttctcgtc cagtcgatgc gctccggcgc gcgacgcagg
     5041 atcgtcagag cgtcggcgac ccagcgtgcc ggcgataccg gcgaccaatg cgcggcgtgc
     5101 gcgcagacga tgacgagcag atccgtcgcg caaggcgcct gcgtcgccac cccgtcgacc
     5161 gtatacggct ccgcagcctg ccaggcaggg tcctcggcgc cggcgaagca tgattccggc
     5221 agcaaatgcc aatgcaggtc gaaatcgaga cgatccggcg actggaagca tgcgctgtga
     5281 tacgcgtccg gcagcatttc ggccatcggc tgagtactgg cccatccggc accaagcaat
     5341 acgcgaacgg cgtcgcgcgc ctgtgtgcgc gggaccagca cgtcgaagtc gttcatcggt
     5401 cgtaagccga ggtcgccgta ataggcgctg gcgaggacgc ctcccttcga caccattgtc
     5461 g
//
LOCUS       DATANS010000551         3739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_364406, whole genome shotgun sequence.
ACCESSION   DATANS010000551 DATANS010000000
VERSION     DATANS010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364406"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VHT22_08100"
     CDS             <1..556
                     /locus_tag="VHT22_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX3137556.1"
                     /translation="AHMGFVTLGFFLFSELGVEGGLIQMLSHGFVSAAMFLCVGVLYD
                     RMHSRQIADYGGVVNTMPRFAALAMLFAMANAGLPGTSGFVGEFMVILAAVKANFWVG
                     FLAAVTLILGAAYTLWMYKRVWFGAVVSEQVAALKDLSRREFWLLATLAAVVLVMGVY
                     PKPFTDVMHASVTDLLAHVAKSKL"
     gene            556..2007
                     /gene="nuoN"
                     /locus_tag="VHT22_08105"
     CDS             556..2007
                     /gene="nuoN"
                     /locus_tag="VHT22_08105"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEX3137557.1"
                     /translation="MTGTPANFDFLPALPEITLLVAACVVLMVDLVLPETRRKWSYWL
                     TQLGVVATAWLSLYVFHESPAHTFGNTFVADALADVLKFLSCVTVALTLAYSRSYLTE
                     RGLFRGETFALMMFALLGMMVMISANHFLLLYLGLELMSLSLYSMIALQRESGQAIEA
                     AMKYFVLGALASGLLLYGMSMIYGATGTLDVARVATTVFAGGVNKTMLAFGLVFVVSA
                     VAFKLGAVPYHMWLPDVYEGAPTAMTLFVGAAPKLAAFAFMTRLLVGSLAAMAFDWQG
                     MLLILSLLSMIAGNIIAISQTNIKRMLAYSTIANMGYMLMGFLAANINGYSAAMFYTI
                     TYVMTTLVSFGMVLLLSRAGFEADQLDDFKGLNQRSPWYAFLMLLTMFSLAGIPPTVG
                     FYAKFAVIEAAVDVGLVWLAVVAVMASLIGAYYYLRIVKLMYFDDPVDSAPLESRSDT
                     KALLSINGLALLLLGIFPQWLLGMCVVALTRSY"
     gene            2057..2779
                     /locus_tag="VHT22_08110"
     CDS             2057..2779
                     /locus_tag="VHT22_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX3137558.1"
                     /translation="MRVGFFVTCLVDLMRPSIGFAAIRLLEAGGAEVFVPPTQTCCGQ
                     PGYNSGDRAGAIALARKLVGEFEACDYLVAPSGSCTGMIKTHYADLFRDDPAMLARAG
                     ALAERTFELTDFLANVLTLDAVPGTYAGTITYHDSCAGLREMGVKQQPRALLARMPGV
                     RLAEMTECETCCGFGGTFSIKYGEIATRMADNKCANIAASGADAVVLGDLGCMLNIEG
                     RLRRRGDSRTRVLHIAELLAGD"
     gene            2797..>3739
                     /locus_tag="VHT22_08115"
     CDS             2797..>3739
                     /locus_tag="VHT22_08115"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEX3137559.1"
                     /translation="MHIASMHFKELAHDNMGNAQLQGTLQKIKGKFVAKRRESLVELD
                     DFEATREAARAIRQRALDNLDVWLQMFERNATARGATVLWAETPADVNRHVLAIAERH
                     GAAKIIKSKSMVSEESALDRAIEAAGKEVVETDLGEYILQINDYEPPSHIIGPALHKS
                     KEEVSDLFAKVHGTAKKMEINELCLEARQVLRKHYLAADMGISGGNFFVAETGSVVLV
                     TNEGNATLTTTLPDVHVAISGIEKIVPTLEDVATLMRLLPRSATGQSIANYVDLLTGV
                     RGEGEFHGARHMYFILVDAGRSAVLGSEVSEALRCIRC"
BASE COUNT          630 a         1188 c         1198 g          723 t
ORIGIN      
        1 cgcgcacatg ggcttcgtca cgctgggatt ctttctgttc agcgagctcg gcgtcgaggg
       61 cgggctcatt cagatgctct cgcatggctt cgtctcggcg gcgatgtttc tttgcgtcgg
      121 cgtgctgtat gaccgcatgc acagtcggca gatcgccgat tacggcggcg tcgtcaacac
      181 aatgccgcga ttcgccgctc tcgcgatgct gttcgcgatg gccaatgccg gtctgcccgg
      241 caccagcgga ttcgtcggcg agttcatggt gatcctcgcc gcggtcaagg cgaatttttg
      301 ggtgggattc ctcgccgcgg tcacgctgat cctcggcgcc gcctacacgc tctggatgta
      361 caagcgggtc tggttcggcg cggtggtaag cgagcaggtc gccgcgctga aggacctgtc
      421 gcggcgagag ttctggctgc tggccacact cgccgcggtg gtcctggtca tgggcgtcta
      481 tccgaagccg ttcaccgacg tcatgcacgc gtccgtcacc gacctgctgg cgcacgtcgc
      541 caagagcaaa ctgtgatgac cggcacaccg gcgaatttcg actttcttcc ggcgttaccg
      601 gagatcacgc tgctggtcgc agcgtgcgtc gtgctgatgg tcgatttggt gctgcccgaa
      661 acccggcgca aatggagcta ctggctgacc cagctcggcg tggtcgcgac cgcgtggctg
      721 tcgctgtacg tattccacga gtcgccggca cacacgttcg gcaatacctt tgtcgccgac
      781 gcgctggccg acgtgctcaa gttcttgagc tgcgtcaccg tcgcgctgac gctcgcgtac
      841 tcgcgcagct acctgaccga gcgcgggctg tttcgcggcg agaccttcgc gctgatgatg
      901 ttcgcgctgc tgggcatgat ggtgatgatt tcggccaatc acttcctgct gctgtatctc
      961 ggactggagc tgatgtcgct gtcgctgtac tcgatgattg cgctgcaacg ggaatcgggc
     1021 caggcgatcg aagcggcgat gaaatacttt gtcctcggcg cgctcgcttc gggcctgctg
     1081 ctctacggca tgtcgatgat ctacggcgcg accgggaccc tcgacgtcgc gcgcgtggct
     1141 acgactgttt tcgccggcgg cgtcaacaag acgatgctgg cattcggcct ggtgttcgtc
     1201 gtgtccgcgg tcgcgttcaa gctgggcgcg gtgccgtatc acatgtggct gcccgacgtg
     1261 tatgagggcg cgccgacggc gatgaccctg ttcgtcggcg cggcccccaa actcgccgcg
     1321 ttcgccttca tgacgcggct tctggtcgga tcgctcgcgg cgatggcgtt cgactggcag
     1381 ggaatgctgc tgatcctttc gctgctgtcg atgatcgccg gcaatatcat cgcgatctcg
     1441 cagaccaaca tcaagcggat gctggcgtac tcgacgatcg ccaacatggg ctacatgctg
     1501 atgggctttc ttgccgcaaa catcaacggc tacagcgcgg cgatgttcta cacgatcacc
     1561 tatgtaatga ccacactggt cagcttcggc atggtcctgc tgctgtcgcg cgccggcttc
     1621 gaagccgacc agctggacga tttcaaggga ctcaaccagc gcagcccttg gtatgcgttc
     1681 ctgatgctgc tgacgatgtt ctcactggcc ggtattccgc cgacggtcgg cttttacgcc
     1741 aagttcgccg tgatcgaggc tgccgtcgac gtaggtctcg tctggcttgc ggtcgtcgcg
     1801 gtgatggcgt cgttgatcgg cgcctactac tatctgcgta tcgtcaagct gatgtacttc
     1861 gacgacccgg tcgacagcgc tccgctggag tcgcgaagcg ataccaaggc attgctgtcg
     1921 atcaacggac tcgcgttgct gctactgggc atctttccgc aatggctgct gggcatgtgc
     1981 gtcgtcgccc tgacgcggtc ctactgattc cgctcggtcg cgagctgtca acgtcgaatt
     2041 gaattccgga tcaaagatgc gcgtcggatt cttcgtcacc tgcctggtcg acctgatgcg
     2101 tccgtcgatc ggcttcgccg cgatcaggct gctcgaagcg ggcggcgccg aagtgttcgt
     2161 gccgccgacg cagacctgct gcggccagcc gggctacaac tccggcgatc gcgccggagc
     2221 gatcgcactg gcgcgaaagc tggtcggcga attcgaagcc tgcgactacc tggtcgcccc
     2281 gtccggctcg tgcaccggca tgatcaagac ccactacgcc gacctctttc gcgacgatcc
     2341 ggcgatgctc gcgcgggccg gagcgctggc ggaacggact ttcgagctga ccgatttcct
     2401 ggcgaacgtg ctcacgctcg acgcggtgcc cggaacgtat gccggcacga tcacctatca
     2461 cgacagttgc gccggactgc gcgagatggg cgtcaagcaa cagccgcgcg cgctgctggc
     2521 gcggatgccc ggcgtcaggc tcgccgagat gaccgagtgc gagacctgct gcggcttcgg
     2581 tggaaccttc tcgataaagt atggcgagat cgcgacgcgc atggccgaca ataaatgcgc
     2641 gaacatcgcg gcctcgggcg ccgacgcggt cgtgctcggt gatctgggct gcatgctcaa
     2701 catcgagggg cggctgcgcc ggcgcggcga cagccgcacc cgcgtactgc acatcgccga
     2761 attgctggcc ggcgactagc ctcaccagga cgcgtaatgc acatcgcctc gatgcacttc
     2821 aaggaactcg cgcacgacaa catgggcaac gcccagctgc agggcaccct gcagaagatc
     2881 aagggtaaat tcgtcgccaa gcggcgcgaa tcgctggtcg agctcgacga cttcgaggcg
     2941 acgcgcgaag ccgcgcgcgc gatccgccag cgcgcgctcg acaacctcga cgtctggctg
     3001 cagatgttcg agcgcaacgc gactgcgcgc ggcgcgacgg tcctctgggc cgagacgccg
     3061 gccgacgtca accggcacgt gctggcgatc gcggagcgtc acggcgccgc caagatcatc
     3121 aagtccaagt cgatggtcag cgaagagtcg gcgctcgatc gcgcgatcga ggctgcgggc
     3181 aaggaagtcg tcgaaaccga cctcggtgaa tacatcctgc agatcaacga ctacgagccg
     3241 ccgtcgcaca tcatcggccc cgcgctgcac aagagcaagg aagaggtgtc cgacctgttc
     3301 gccaaggtgc acgggaccgc gaaaaagatg gagatcaacg agctctgcct cgaggcgcgc
     3361 caggtgctgc ggaagcacta tctggccgcc gatatgggca tctcaggagg caattttttc
     3421 gtcgccgaga ccggctcggt cgtgctggta accaacgagg gcaacgcaac gctgacgacg
     3481 accttgcccg acgtccacgt cgcgatctcc ggcatcgaga agatcgttcc gacactcgag
     3541 gacgtggcga ccctgatgcg cctgctgccg cgctcggcga ccggacagtc gatcgccaac
     3601 tatgtcgacc tcttgaccgg cgtccggggc gaaggcgaat ttcacggcgc ccggcacatg
     3661 tacttcatac tcgtcgacgc cggacgaagc gcggtgctcg gcagcgaagt cagcgaggcc
     3721 ctgcgctgca tacgctgcg
//
LOCUS       DATANS010000552         1530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_701526, whole genome shotgun sequence.
ACCESSION   DATANS010000552 DATANS010000000
VERSION     DATANS010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701526"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VHT22_08120"
     CDS             <1..614
                     /locus_tag="VHT22_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3137560.1"
                     /translation="AGKSTLFNLIAGVLPPTQGSILFDGEDVTALPAAERCQRGIART
                     FQVVKSFETMTVIDNVIVGALIRTTVMREARRKAHEVLEFAGLAGRADALASELIPSE
                     KRRLEVARALATEPKLLLLDEVLTGLTPIEAQSGVELVRKVRATGVTVLMVEHVMEIV
                     MPLVDRAIVLDLGKVLVEGKPADIVRDPKVITAYLGDRHAVGA"
     gene            598..1302
                     /locus_tag="VHT22_08125"
     CDS             598..1302
                     /locus_tag="VHT22_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3137561.1"
                     /translation="MLSVREVTTAYQGLVAISAVSIEVAKGEIVCVAGANGAGKSTLL
                     KSIAGAERPRSGTITFDGARIDGLAQHMITARGIAYVPENRRLFPRLSVRDNLRLGSY
                     LYRSEADRDQPLALVFKLFPRLSERLEQRAETLSGGEQQMLAIGRALMTRPRLLMLDE
                     PSQGIMPKLVDEIFAAVKRIRDAGMTVLIVEQRMAECLDIADRAYILQTGRVLMQGAA
                     SEIKGNPDVRKADLGL"
BASE COUNT          259 a          498 c          521 g          252 t
ORIGIN      
        1 gcgccggcaa gtcgacgctg ttcaatttga tcgcgggcgt gctgccgccg acccagggtt
       61 cgatcctgtt cgacggcgag gacgtcaccg cgctgccggc ggcggagcgc tgccagcgcg
      121 gcatcgcgcg caccttccag gtcgtgaaaa gcttcgagac catgacggtc atcgataacg
      181 tcatcgtcgg cgcgctgatc cgcaccactg tcatgcgcga ggcgcgccgc aaggcccacg
      241 aagtgctgga atttgcaggc ctggccgggc gcgccgatgc gctcgccagc gagctgatcc
      301 cgtcggaaaa gcgccggctc gaggtcgcgc gcgcgctcgc gaccgaaccg aaactgctgc
      361 tgctcgacga ggtgctcacc ggcctgacgc cgatcgaggc gcaaagcggc gtcgaactgg
      421 tgcgcaaagt gcgcgctacc ggcgtcaccg tcctgatggt cgagcacgtc atggagatcg
      481 tgatgccgct ggtcgaccgc gccatcgtgc tcgacctcgg caaggtgctg gtcgagggca
      541 agcctgccga catcgttcgc gatccgaaag tcatcaccgc ctatcttggg gaccgccatg
      601 ctgtcggtgc gtgaagtcac caccgcctat caggggctgg ttgcgatctc ggcggtctca
      661 atcgaggtcg ccaagggcga gatcgtctgc gtcgccggcg ccaacggggc cggcaagtcg
      721 acgctgctga aatcgatcgc gggcgccgag cggccgcgct ccggcaccat caccttcgat
      781 ggcgcgcgca tcgacggcct ggcgcagcat atgatcaccg cgcgcggcat cgcctatgtg
      841 ccggagaacc gccggctgtt tccgcggctg tcggtgcgcg acaatctgcg gctcggcagc
      901 tatctctatc gcagcgaggc cgaccgcgac cagccgctgg ccctggtgtt caagctgttt
      961 ccgcgattgt cggagcggct ggagcagcgc gccgaaacgc tctcgggcgg cgagcagcag
     1021 atgctggcga tcgggcgcgc gctgatgacg cggccgcggc ttttgatgct ggacgaaccg
     1081 tcgcagggca tcatgccgaa gctggtcgac gagatctttg ccgcggtaaa gcgcatccgc
     1141 gacgccggca tgaccgtgct gatcgtcgag cagcgcatgg ccgaatgtct cgacatcgcc
     1201 gaccgcgcct acatcctgca gaccggccgc gtgctgatgc agggcgcggc cagcgagatc
     1261 aagggcaatc ccgacgtgcg caaggcggat ctggggctgt gatctcaacg gtatcgccgc
     1321 atacactagc gtcatcctgg ggtgcgcgct cttgcgcgcc tcgaaggatg gacggcagac
     1381 acggacccca atcatccttc gaggctcgcc gaagtcggcg agcacctcag gatgacgctg
     1441 gtgggtgagg ctcgacggta gccccaccgt cgtcattgcg aggagcgcag cgacgaagca
     1501 atccattctt tcttgcgcgg agagatggat
//
LOCUS       DATANS010000553         2517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_449129, whole genome shotgun sequence.
ACCESSION   DATANS010000553 DATANS010000000
VERSION     DATANS010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449129"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VHT22_08130"
     CDS             <1..600
                     /locus_tag="VHT22_08130"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020809832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016993 - precorrin-8X methylmutase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HEX3137562.1"
                     /translation="AIYERSFAIIRAEADLSRFTPEEAEVAVRMIHAAGQVEVAGLIE
                     FAPEFVSAARKALRGGAPILCDSEMVAHGVTRARLPAKSEVICTLRDPRVPDLAAKLG
                     TTRSAAALELWRDKLAGAVIAIGNAPTALFRLLEVLEAGAAKPAAIIGIPVGFVGAAE
                     SKEALAANVLGVPYLIVRGRIGGSAMTAAAVNALAKEGI"
     gene            597..1325
                     /locus_tag="VHT22_08135"
     CDS             597..1325
                     /locus_tag="VHT22_08135"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HEX3137563.1"
                     /translation="MTTGRLIGVGVGPGDPGLLTLNAIAALNAADVIAHFAKAGNASN
                     ARTIAAKHLKPGVEELSLLYPLTTEIPKEDAAYRETLREFYDSAAAQIAGHLDQDRTV
                     AVIAEGDPLFYGSYMHLHVRLAPRYPTEVVAGVTGMSGCWSAIGEPIAQGNDVFTVLP
                     GTLPEYELERRLADADAAVVMKIGRHLAKVRRALDRAGRLERAIYVERGTMTNAAVMP
                     LVDKLDTYAPYFAIVLVPGWEARP"
     gene            1322..2086
                     /locus_tag="VHT22_08140"
     CDS             1322..2086
                     /locus_tag="VHT22_08140"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007695080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HEX3137564.1"
                     /translation="MSGKLSIVGLGPGDDRYLTREALDALAKAEALYGYGPYLDRVPS
                     RKGQSRHASDNREEAARARAALSRAASGANVAIVSGGDPGVFAMAATVCEEIAVGPDS
                     WRALDVAIVPGITAMLAVAARAGAPLGHDFCALSLSDNLKPWELIERRLDAAAGAGFV
                     IALYNPVSRARPLQLSKALARLARHLPAATPVIFGHAIGRSDESLAVTTLAHAGDTLA
                     DMATLIIVGSRETRVIAREGRAPLVYTPRAVEEAIA"
     gene            complement(2050..>2517)
                     /locus_tag="VHT22_08145"
     CDS             complement(2050..>2517)
                     /locus_tag="VHT22_08145"
                     /EC_number="1.3.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A/cobalt-precorrin-6A reductase"
                     /protein_id="HEX3137565.1"
                     /translation="RKAWTKVDGDSWVEVATMDEAVRALGQAPRRVFLALGRKELQPF
                     TAAPQHVYLIRSVDPVDPPLAVPHAIYIAARGLFSEAEDRALLKRHRIDVVVAKNSGG
                     SATYGKIAAARTLQLPVIMLARPALPEVSSVATVEEALVWLDHAIASSTARGV"
BASE COUNT          410 a          874 c          812 g          421 t
ORIGIN      
        1 gccatctatg agcgctcctt cgcaatcatc cgcgcagagg ccgacctctc gcgcttcacg
       61 ccggaagaag ccgaggtcgc agtgcgcatg atccacgccg caggtcaggt cgaagtcgcc
      121 ggcttgatcg aatttgcgcc cgagttcgtg tcagcagccc gcaaggcgtt gcgcggcggc
      181 gcaccgatcc tgtgcgactc cgagatggtt gcgcacggcg tcactcgcgc gcggctccct
      241 gccaagagcg aagtcatctg cacgttgcgc gacccacgcg tcccggatct tgccgcgaaa
      301 ctcggcacta cgcgctctgc cgccgcgctc gaactgtggc gcgataagct tgcgggcgct
      361 gtgatcgcca tcggcaatgc gccgaccgcg ctatttcgcc tgctcgaagt gttggaggcg
      421 ggtgccgcca agccggctgc gatcatcggc attcccgtag gcttcgtcgg cgcagccgaa
      481 tcgaaagagg cgctcgcggc gaacgtgtta ggcgtgcctt acctcatcgt gcgtggccgc
      541 atcggcggca gcgctatgac ggcggccgca gtcaatgcac tcgccaagga gggaatatga
      601 ccacggggcg cctcatcggc gtgggcgtag ggcccggcga tccagggctg ctcacgctga
      661 acgctatcgc tgcgctcaat gcagccgacg tgatcgccca tttcgccaag gcaggaaacg
      721 cgagcaacgc gcgaaccatc gcggcaaagc acctcaaacc tggcgtcgag gaactgtcgt
      781 tgctctatcc gctgacgacc gagatcccca aggaggatgc agcctatcgt gaaacgctgc
      841 gcgagttcta tgatagtgcg gctgcacaaa tcgccgggca tctcgaccaa gaccgcacag
      901 tcgccgtgat cgccgagggc gatcccctgt tctacggctc ctacatgcat ctccatgtgc
      961 ggctcgcccc gcgctatccg accgaggtgg tcgccggggt gaccggcatg tccggctgct
     1021 ggtcggccat cggcgagcca attgcgcaag gcaacgacgt gttcaccgtg ctgcccggta
     1081 cgctgcccga gtacgagctc gagcgccgcc tcgccgatgc cgacgccgcg gtggtgatga
     1141 agatcggccg ccatctcgcc aaggtgcgac gcgcgctcga tcgcgccggc cggctcgaac
     1201 gcgcgatcta cgtcgaacgc ggcaccatga ccaacgccgc tgtcatgcct ctcgtcgaca
     1261 agctcgatac ctatgcgccc tacttcgcca tcgtgctggt gccgggctgg gaggcgcggc
     1321 catgagcggc aagctctcaa tcgtcgggct cgggcccggc gatgaccgct acctcacgcg
     1381 cgaggcgctc gatgcgctcg ctaaagctga agcgctctac ggctatgggc cttatctcga
     1441 ccgcgttccg tcgcggaaag gccaatcgcg gcatgcttcc gacaatcgcg aggaagcggc
     1501 acgcgcccgc gccgcgctaa gccgcgcggc gtcaggcgcc aatgtagcca tcgtgtcagg
     1561 cggggaccct ggcgtattcg ccatggcggc tacggtgtgc gaggagattg cggtcggccc
     1621 cgactcgtgg cgcgcgctcg atgtcgccat cgttcccggc attactgcca tgcttgcggt
     1681 cgctgcccgc gcaggagcgc cgctcgggca cgatttctgc gcgctgtcgc tgtcggacaa
     1741 tctcaagccg tgggagctga tcgagcgtcg cctcgatgcg gccgccggcg ccggcttcgt
     1801 catcgcgctt tacaatcctg tctcccgcgc gcgaccgtta cagctcagca aggcgctcgc
     1861 gcggctcgcc cgccatctgc cggctgcgac gcccgtcatc ttcggtcatg cgattgggcg
     1921 cagcgatgaa agcctcgcgg taaccacgct cgctcacgca ggcgacacgc ttgccgacat
     1981 ggcgacgctc atcatcgtgg gatcgcgcga gacgcgcgtc atcgcccgcg agggccgtgc
     2041 gccgctcgtc tacacgccgc gtgccgtcga ggaggcgatc gcatgatcga gccagacgag
     2101 cgcctcctct accgtcgcga cggaggaaac ctctggcagc gccgggcgcg ccagcatgat
     2161 caccggaagc tgaagcgtcc gcgccgcggc gatcttgccg taggtcgcgc tcccgccgct
     2221 gttcttcgcc accaccacat caatgcggtg gcgcttgagc agcgcgcggt cctcggcctc
     2281 gctaaacagt ccgcgcgcgg cgatatagat tgcgtgcggc acggcgagcg gcgggtcgac
     2341 aggatcgacg ctgcggatga gatagacgtg ctgcggcgct gccgtgaaag gctgaagctc
     2401 tttccggcca agcgcgagga acacgcggcg cggcgcctgc ccgagcgcgc gcaccgcctc
     2461 atccatcgtc gcaacttcga cccagctatc tccatcgacc ttggtccacg ccttacg
//
LOCUS       DATANS010000554         1914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_823162, whole genome shotgun sequence.
ACCESSION   DATANS010000554 DATANS010000000
VERSION     DATANS010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823162"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="VHT22_08150"
     CDS             complement(<1..1300)
                     /locus_tag="VHT22_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX3137566.1"
                     /translation="MTDPMVPEAIARDSRSQGEGFDWSLPYRSRRQPIFARNVVATSQ
                     PLATQAGIAMLQRGGNAVDAALATAIALTVVEPCSNGLGSDLFAIVWDGAALSGLNAS
                     GRAPEAWTPERFAGLDAMPRAGWDTITIPGAVSGWVALSRRFGKLPFADLFTPAIRYA
                     GEGYAVSPVVAEKWAKAVPILGQVEGFAEHFLPRGRAPAPGEIFSSAAMARSLQKIAA
                     SGGEAFYRGELAEAMVAHARANRGAHTLADFDAHRCDWVTPLAHDYHGHTIHELPPNG
                     QGIAALMALGIIENFDLGAHPVDAVATQHLEIEAMKLAFADVYRYVSDPASMRVTPAQ
                     LLDRGYLSARARLIDLGRAQDFGYGTPPPGGTVYLAAADESGMMVSLIQSNYMGFGSG
                     IVVPGTGISMQNRGTGFSLKPDHPNLVGGGKRPFHTIIPGF"
     gene            complement(1329..>1914)
                     /locus_tag="VHT22_08155"
     CDS             complement(1329..>1914)
                     /locus_tag="VHT22_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX3137567.1"
                     /translation="NAGAQTSFIPLLTLGIPENAVMALMVGAMTIHNIQPGPQVMTSN
                     PSLFWGLVASMWVGNLMLVVLNLPMIGIWIKLLQVPYRLLYPAILLFCGIGVYSTNNA
                     SFDVVMTAIFGFLGVIFVKLECEPAPLLLGFVLGPLMEENLRRAMLLSRGDPTVFLTR
                     PISLAMLIVAAGLLLLIILPNFRKTRAAAFQEET"
BASE COUNT          365 a          622 c          624 g          303 t
ORIGIN      
        1 cgaagccggg gatgatcgtg tggaacggac gcttgccgcc gccgaccagg ttcggatgat
       61 ccggcttcag tgaaaatccg gtgccgcgat tctgcatgct gatgccggtg ccgggcacga
      121 cgatccctga accgaagccc atgtagttcg actggatcag cgacaccatc atgccgcttt
      181 cgtccgccgc agcgagatag acggttccgc cgggcggcgg cgtgccgtac ccgaagtcct
      241 gcgcgcggcc gagatcgatc agacgcgccc gcgccgacag atatccgcga tcgagcagct
      301 gggctggtgt cactcgcatc gacgccgggt cgcttacgta gcgatagaca tcggcgaagg
      361 ctagcttcat cgcctcgatt tccagatgct gcgtcgcgac agcatcgacc gggtgcgcac
      421 cgagatcgaa attctcgatg atgccgagcg ccatcagcgc cgcgatgccc tggccattgg
      481 gcggcagctc atggatcgta tggccatggt aatcgtgcgc cagcggcgtt acccagtcgc
      541 accgatgcgc atcgaaatcg gcaagggtgt gggcgccccg gttcgcgcgg gcgtgcgcga
      601 ccatcgcttc cgccagctcc ccgcgataga acgcctcgcc gccgctggcg gcgattttct
      661 gcagcgatcg cgccatcgcc gcggaagaaa aaatctcccc gggcgccggc gcccgcccgc
      721 gcgggagaaa gtgctcggcg aagccttcca cctggccgag tatcggcacg gccttggccc
      781 acttctcggc aacgaccggc gaaaccgcat aaccttcgcc ggcgtaacgg atcgcaggcg
      841 tgaacaggtc ggcaaaaggg agcttgccga accggcgcga cagcgcgacc cagccggaca
      901 cggcgccggg aatggtgatc gtgtcccaac cggcgcgagg catcgcgtca aggccggcga
      961 agcgctccgg cgtccacgcc tcgggtgcgc gtcctgaagc gttgagcccg gagagcgcgg
     1021 cgccgtccca tacgatcgcg aacaggtcgc tgcccaggcc gttgctgcag ggctcgacga
     1081 cggtgagcgc gatcgcggtt gccagcgccg cgtccacggc gttgccgcct ctttgcagca
     1141 tcgcgatccc tgcctgcgtc gcaagcggct gcgaggtcgc cacgacattg cgcgcgaaaa
     1201 tcggctgccg gcgcgaccgg tatggcaggc tccagtcgaa gccctctccc tggctgcggg
     1261 aatcgcgtgc gatcgcttcc ggcaccatcg gatcggtcat cgtcgtctca cttgcgcgtt
     1321 tgcgcacatc aggtctcttc ctggaacgcg gcggcgcgcg tcttgcggaa gttcggaaga
     1381 atgatcagca gcagcaagcc cgccgcaacg atcagcatcg ccagcgaaat gggccgggtc
     1441 aaaaagaccg ttggatcgcc gcgcgagagc agcatcgcgc gacgcagatt ttcctccatc
     1501 aggggtccga gcacaaaccc gagcagcaac ggggcgggct cgcattcgag cttgacgaag
     1561 atgaccccga ggaagccgaa aatcgcggtc atcaccacgt cgaagctcgc attgttggtc
     1621 gagtagacac cgatgccgca aaaaagcagg atcgccggat aaagcagacg atacggcacc
     1681 tgcagcagct tgatccagat gccgatcatc ggcaggttca ggacgaccag catcagattg
     1741 ccgacccaca tcgatgcgac caatccccag aacagtgagg ggttggaggt catcacctgg
     1801 ggccctggct ggatgttgtg gatggtcatc gcgccgacca tcagggccat cacggcgttc
     1861 tccgggatgc cgagggtaag caaggggatg aacgacgtct gcgcaccggc gttg
//
LOCUS       DATANS010000555         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_197715, whole genome shotgun sequence.
ACCESSION   DATANS010000555 DATANS010000000
VERSION     DATANS010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197715"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VHT22_08160"
     CDS             complement(<1..641)
                     /locus_tag="VHT22_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX3137568.1"
                     /translation="MTPGSLPPEHTPLPGGVVRGHAQQRQQLTLQHYGWIIGVTVIVA
                     LLLHLLGDILTPFLIGAILAYIGNPAVSWGETHRVSRTIGTLLVVGVTGLAVFGLILV
                     LTPLVHSEFSQLVQRLPTLAVLLQTKFAPWLQQTFGIELQLDMDSIKAMISDNLAGAE
                     ALTPKVLSSVKAGGTLILAILINLALIPVVMFYLLRDWNIILARFEELTPRRWL"
     gene            736..1800
                     /gene="purM"
                     /locus_tag="VHT22_08165"
     CDS             736..1800
                     /gene="purM"
                     /locus_tag="VHT22_08165"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX3137569.1"
                     /translation="MNAPKSVPGRPLSYRDAGVDIDAGDRLVEAIKPFAKRTLRPEVL
                     AGIGGFGALIELPKRYREPVLVAGTDGVGTKLKLAFAMNKHDTIGIDLVAMSVNDILV
                     QGAEPLFFLDYYVCETLDVAIASDVIRGIARGCELAGCALAGGETAEHPKAFVPGEYD
                     LAGFAVGVVEKSRAIDGRGIAAGDVLLGLASSGPHSNGFSLIRGILERGHADLAQKIA
                     GVTGERTLGETLLEPTRIYVKPVLALLGECEIKGMAHITGGGLSENTHRMFPEGLAAR
                     IDAQSWPRPPIFDWLQRQGNVAAAEMFRVFNCGIGFVLVVAPEAAAAATARLSASGET
                     VYAIGTVVPRAADAPGTVVA"
     gene            1931..2599
                     /gene="purN"
                     /locus_tag="VHT22_08170"
     CDS             1931..2599
                     /gene="purN"
                     /locus_tag="VHT22_08170"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX3137570.1"
                     /translation="MKRLVILISGRGSNMAALIDAVRAGEIDAAIGTVISNRPDAAGL
                     GIAAAAGVATSVVDHRAFADRGEFERSLIAAIDAKAPDLVVLAGFMRILSPAFVTRYA
                     GRLLNIHPSLLPAYPGVDTHRRALADGVRIHGCTVHFVTSDVDCGPIVAQAAVTVRQD
                     DDEAALAARVLAVEHRLLVGAVRWFCSDRLSIEGSRVRIRGEIADPAVLQAPSDKATN
                     SPPS"
     gene            2720..>2990
                     /locus_tag="VHT22_08175"
     CDS             2720..>2990
                     /locus_tag="VHT22_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX3137571.1"
                     /translation="MASLAVHIVLLQSTIRLPDRIEESPPLLATITELPPPPAPVATP
                     SPVKPKHRRVVRAAPPVPVETPAPEVVAAATPVPEPVREPVAPPPL"
BASE COUNT          478 a         1050 c          970 g          492 t
ORIGIN      
        1 agccagcgcc gcggggtcag ctcctcgaac cgcgcgagga tgatgttcca gtcgcgaagc
       61 agatagaaca tcaccaccgg gatcagcgcc aaattgatca ggatggcgag gatcagcgtg
      121 cctccggcct tgacgctcga cagcaccttc ggggtgagcg cttccgcgcc cgccagattg
      181 tccgaaatca tcgccttgat cgagtccata tcaagttgca gttctatccc gaacgtctgc
      241 tggagccacg gcgcgaactt ggtttgcagc agcacggcga gggtgggcaa ccgctgcacc
      301 agctgtgaaa actcggagtg cacgagcggc gtcagcacca ggatcagccc gaacaccgcg
      361 aggccggtga cgccgaccac cagcagggtc cctatcgtgc gcgatacgcg atgcgtttcg
      421 ccccagctta cggcgggatt gccgatgtac gccaggatcg cgccgatcag gaacggtgtg
      481 aggatgtcgc ccagcaggtg cagcagcagc gccacgatca ccgtgacgcc gatgatccat
      541 ccgtagtgct gcagagtcag ctgctggcgc tgctgcgcgt gaccgcgaac gactccgccc
      601 ggcaacggcg tatgttcggg cggcaggctc cctggtgtca tttccggtaa aatctccccc
      661 gcgcacttta cagggcgcga cgccgcgact caatccaatt cgccttccta caccgccgcg
      721 acggccgaga ccgcattgaa cgcccccaaa agcgtccccg gccgtccgct ttcgtatcgc
      781 gacgcgggcg tcgacatcga tgccggcgac cggctggtcg aggcgatcaa gccatttgcc
      841 aagcgcacgc tgcggcccga ggttctggcc ggcatcggcg gttttggtgc gctgatcgag
      901 ctgccgaagc gctaccgcga gccggtgctg gtcgccggga ccgacggcgt cggcaccaag
      961 ctcaagctcg cattcgcgat gaacaagcac gacaccatcg gtatcgacct ggtcgcgatg
     1021 agcgtcaacg atatcttggt ccagggcgcc gagccgctgt tctttctcga ttactacgtc
     1081 tgcgaaacgc tcgatgtcgc gatcgccagc gacgtcatcc gcggcattgc tcgaggctgc
     1141 gagctggccg gttgcgcact cgccggcgga gaaaccgccg agcacccgaa ggcattcgtt
     1201 cccggcgaat acgatctcgc cggattcgcc gtcggcgtcg tcgaaaaatc gcgcgcaatc
     1261 gacggccgcg ggatcgctgc cggcgacgta ttgctcgggc tcgcctccag cggaccgcat
     1321 tcgaacggct tttcgctgat ccgaggcatt ctggagcgcg ggcacgccga cctcgcgcaa
     1381 aagatcgccg gcgtcaccgg cgagcgcacg ctcggcgaga cgctgctcga gccgacgcgc
     1441 atctatgtca agccggtgct ggcacttctc ggcgagtgcg agatcaaggg aatggcgcac
     1501 atcaccggcg gcggcttgag cgagaacacg caccggatgt tccccgaagg cctcgcggcg
     1561 cgcatcgacg cgcaatcgtg gccgcggcca ccgatcttcg actggctgca gcggcaaggc
     1621 aacgtcgctg ccgccgagat gttccgcgtg ttcaattgcg gcatcggctt cgtgctggtc
     1681 gtcgcgcccg aggcagccgc cgcggcgacc gcgcgactgt ccgcatcggg cgagacggtg
     1741 tacgccatcg gcaccgtcgt gccgcgcgcc gccgatgcgc cgggaacagt cgtcgcgtga
     1801 gacttgagtc cgtgcgctcg cgcagtggag atgacaccaa cgtcatcccg gtggaggccg
     1861 ggatccaggc tccgatttcg tgctcggcgc gcgtcgctgc ctgggccccg gcctgcgccg
     1921 gggcgacgat gtgaaacgcc tcgtcatcct catctccggc cgcggctcca acatggccgc
     1981 cctgatcgac gcggttcgag cgggcgagat cgacgcggcg atagggaccg tgatcagcaa
     2041 ccggcccgat gcggcgggac tcggaatcgc cgctgcggcc ggagtcgcta cgtcggtcgt
     2101 cgatcatcgc gctttcgccg accgcggcga gtttgagcgc tcgcttatcg cagccattga
     2161 tgcaaaagcg cccgacctgg tcgtgctcgc gggcttcatg cggatcctct cgcctgcgtt
     2221 cgtcacgcgt tacgcgggcc ggctgctcaa catccaccct tcgctgctgc cggcctatcc
     2281 gggtgtcgac acccatcgcc gggcgctggc agacggggtg cgcatccacg gctgcaccgt
     2341 gcacttcgtc acctccgacg tcgattgcgg gccgatcgtc gcgcaagccg cggtaacggt
     2401 ccgacaggac gacgacgagg ctgcgctggc cgcgcgcgtg ctggcggtcg agcaccgact
     2461 gctggtcggc gcggtgcgct ggttctgctc cgaccggcta tcgatcgagg gctctcgcgt
     2521 gcgcatccgg ggcgagatcg ccgacccggc agtgctgcag gcgccctcgg acaaggcgac
     2581 gaactccccg ccgtcctgac cgtccaaccc ggcaataccc ggccaaagcc ggtaagctcc
     2641 cactgacatg tcctcccgca tcgcatcttt tgcgtctttc gacccgcgac ctggctcgcg
     2701 cgtcctcgcg gtcgcggcgt tggcgtcgct tgcggtgcac atcgtgctgc tgcagtcgac
     2761 gattcggctg cccgaccgca tcgaagagtc gccaccgctt ctggcgacga tcaccgagct
     2821 gccgccaccc ccggcgccgg tcgcaacgcc ttcgccggta aagcccaagc atcgccgcgt
     2881 cgttcgcgcc gcgccgccgg tcccggtcga gactcccgcg cccgaagtcg tcgcagccgc
     2941 gacgccggtg cccgagcccg tgcgagagcc cgtcgctccg ccgccgctcg
//
LOCUS       DATANS010000556         2479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_613406, whole genome shotgun sequence.
ACCESSION   DATANS010000556 DATANS010000000
VERSION     DATANS010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613406"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VHT22_08180"
     CDS             complement(<1..883)
                     /locus_tag="VHT22_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX3137572.1"
                     /translation="MPKCSDGTIGFGRLGRRVIEANFDGGDISSDGGVLLLRRADERI
                     GLSRAAAAVLSDPRDPRRITHALRDLLAQRIYGLCCGYEDLNDHDALRDDLLMQTAVG
                     RVDALASSPTLSRLETRATRAQAVALHGVLVEQFIASHKTAPAELVLDIDASDVALHG
                     NQELSEFHAYYDSHCYLPLYVFCGQAMLACVLRRSRIDGAKNAAAVIKLLVQRLRRAW
                     PDTRIIVRADSGFCRQRLLRWCERSGVSYIIGLARNARLEARVRLAELMLADEYERSG
                     AKQRLIDEFAYAADSWNT"
     gene            complement(974..2053)
                     /locus_tag="VHT22_08185"
     CDS             complement(974..2053)
                     /locus_tag="VHT22_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX3137573.1"
                     /translation="MRADAEIELTEEQRSELEQVARSRRTPQAVALRARIVLLTADGF
                     APSAIAEELGTSQPTVRKWRARYVEDGMAGLRNEPRPGRPRSLDDQRVADLLNQALQT
                     RPAKQTHWSVRSFAAEAHISKDMAHRLFRTAGIAPHRSRSFKLSNDPAFVEKVRDITA
                     LYLNPPDHALVLCVDEKSQIQALERTQPLLPMGFGYVEGVTHDYVRHGTTTLFAALDV
                     ANGQVISRVRTQHRHQEFLDFLRQIDKQTPPDLDLHLIADNYITHKHAKVKAWLARNP
                     RVHMHFTPTYSSWINQVERWFALITERAIRRNSFTSVRELKQQIELFVQRHNADTTPF
                     KWVATADSILQKVEKIAKRISATAH"
BASE COUNT          432 a          813 c          794 g          440 t
ORIGIN      
        1 gggtgttcca gctgtcggcc gcgtaggcga actcgtcgat cagccgctgc ttggcgccgc
       61 tgcgctcgta ctcgtcggcg agcatcaatt cggccaagcg aacccgtgct tccagccgcg
      121 cgttgcgagc cagcccgatg atatagctca cgcccgagcg ctcacaccag cgcagcaatc
      181 tctggcggca gaaccccgag tcggcgcgca cgatgatgcg tgtatcaggc caggcccggc
      241 gcagccgctg caccagcagc ttgatcacgg ccgcagcgtt cttggccccg tcgatgcgac
      301 tgcgcctgag cacgcacgcc agcatcgcct ggccgcagaa cacgtacagc ggcaagtagc
      361 agtgagagtc gtaataggcg tggaactccg agagctcctg gttgccgtgc agcgccacgt
      421 cggaggcgtc gatgtcgagc accagttcag ccggcgccgt cttgtggctg gcgatgaact
      481 gctcgacgag cacgccgtgc agcgccaccg cctgcgcccg cgtggcgcgc gtctccaggc
      541 ggctcagcgt gggcgagctc gccaacgcat cgacccggcc gaccgcggtt tgcatcagca
      601 agtcgtctcg cagcgcgtcg tggtcgttga gatcttcgta gccgcagcac agcccgtaga
      661 tgcgctgcgc cagcaggtct cgcaacgcgt gcgtgatgcg cctcggatcg cgcggatcgc
      721 tcagcaccgc cgcagccgcg cgactcagcc cgatgcgctc gtcggcgcgt cgcaacagca
      781 gcacaccgcc atccgagctg atgtcgccgc catcgaagtt ggcctcgatc acgcgtcgac
      841 caagccgtcc aaaacctatc gtcccgtcgc tacactttgg catcggcagt cctcggttgt
      901 cacaggcgtc aagtacctat gacaacgcgc gcaagcacga ggctgccgac cctcttctca
      961 tgaaatatcc gggctagtgt gctgtcgcag aaatacgttt cgctattttc tcgaccttct
     1021 gcagtatcga gtccgcggtg gcgacccact tgaacggcgt ggtgtcggcg ttgtgacgct
     1081 gaacgaatag ctcgatctgt tgcttgagtt cgcgcacgct ggtgaaggag ttgcgccgga
     1141 tggcgcgctc ggtgatgagg gcgaaccagc gctccacttg gttgatccac gagctgtagg
     1201 tgggcgtgaa gtgcatgtga acgcgtgggt tgcgtgccag ccacgccttg accttggcat
     1261 gcttgtgggt gatgtagttg tcggcgatca ggtgcaggtc caaatcgggc ggcgtttgct
     1321 tgtcgatctg gcgcaggaag tcgaggaact cctgatgccg gtgctgagtt cgaactctcg
     1381 agatcacctg cccgtttgca acatcgagtg cagcaaacaa tgtcgtggtg ccatggcgaa
     1441 cgtagtcatg ggtgacgccc tcgacgtagc caaagcccat cggcagtagc ggttgcgtgc
     1501 gctccagcgc ctggatctgg ctcttctcgt ccacgcacaa caccagcgcg tgatccggcg
     1561 ggttcaggta cagcgcggtg atgtcgcgca ccttctccac gaacgccggg tcgttcgaca
     1621 gcttgaagct gcgtgaacga tgcggagcga tcccggccgt gcgaaacagg cggtgcgcca
     1681 tgtccttgct gatgtgggct tcggccgcga agctgcgaac gctccaatgg gtttgcttgg
     1741 ccggtcgggt ctgaagagcc tggttcaaca agtccgccac gcgctgatca tccagcgacc
     1801 tcggtcgacc cgggcgcggt tcgttgcgca atcccgccat gccatcctcc acatacctgg
     1861 ctcgccactt gcggaccgtc ggctgcgagg tcccaagctc ctcggcgatc gcgctggggg
     1921 caaacccatc agcggtcagc aagacgatcc gagcacgcaa cgcgacagcc tgcggcgtgc
     1981 gcctcgagcg cgcgacctgc tccagctcgc tacgctgctc ctcggtgagt tcgatctcgg
     2041 cgtccgcacg catctggcgc atggtcccct ccggtcatca atgtaccgga ggacaatgca
     2101 agaatcatgc aacgtactta tgcgacggga cactagctcg ccctggcctt ggcgactgag
     2161 ttcatggtcg cagccgagac gaagaaccgc cgagtggagc accaccgaag ggcagctgat
     2221 ctccttcggc tggagatgaa cgagtcgacc aagagttccg ccgcgccgcc cggccggaac
     2281 cgagcggcgt ctggtgacga accgcgcctg cccgccgcgt cacgatggtc gccgcggcga
     2341 cgggggcgcg tcgcagcgag cgcaaggcga ctactgagtg acgcaggcaa acgacgcggc
     2401 ggcgttgacg ctaccaccca cgtagcgcgg ccacttcggg tagtcgcaca gcggccgact
     2461 gcggcccggc acgccgacc
//
LOCUS       DATANS010000557         1702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_531906, whole genome shotgun sequence.
ACCESSION   DATANS010000557 DATANS010000000
VERSION     DATANS010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531906"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1702
                     /locus_tag="VHT22_08190"
     CDS             <1..>1702
                     /locus_tag="VHT22_08190"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX3137574.1"
                     /translation="AVKPRSSKPPMMRQYDLIERVKRYNPATSEALLNRAYVYAMKAH
                     GEQKRASGDPYFSHPLEVAAILTDLKLDDATIAAALLHDTIEDTAATRAEIDQMFGQD
                     IGTLVEGLTKLKRLDLVTKEAKQAENLRKLLLAIADDVRVLLVKLADRLHNMRTLSHM
                     PPEARRRSAEETLDIYAPLAGRMGMHEMREELEDLAFHELNPEAYTVVSERLDALEAK
                     NVGLVTEIEQQLKRKLLDRGIDAEVSGRRKRAYSIWRKMERKSIGFEQLSDIFGFRVI
                     VKTLPECYQALGIVHTTWPVVPGRFKDYVSTPKQNDYRSIHTTVIGPSQQRVELQIRT
                     KEMHQIADYGIAAHALYKDDVDSPTELLSRESSAYAWLRRTIELLAEGSNPEEFLEHT
                     KLELFHDQVFCFTPKGKLIALPRHANLIDFAYAVHTDVGNMAVGGKINGKIAPLVSEL
                     TNGDEVEIITSRAQHAPPAAWESLVTTGKARAAVRRATRVAVRNQYAGLGRRIVERLC
                     QRAKIAYSDEKLQGALPRLARASIEEVLSSVGRGEMRAADVVRAMYPDYKEERAATIA
                     Q"
BASE COUNT          335 a          572 c          537 g          258 t
ORIGIN      
        1 gcagtaaagc ctaggtcgtc aaagccgcca atgatgcggc aatacgatct gatcgagcgc
       61 gtgaagcgct acaaccccgc gaccagcgag gcgctgctca accgcgccta cgtctacgcc
      121 atgaaggcgc acggcgagca gaagcgtgcg tccggcgatc cctacttctc gcatccgttg
      181 gaagtcgcgg cgatcctgac cgacctgaag ctcgacgacg ccaccatcgc tgcggcgctc
      241 ctgcacgaca ccatcgagga taccgccgct acccgggccg agatcgacca gatgttcggg
      301 caggacatcg gcacgctggt ggaaggcctc accaagctca agcggctcga cctcgtcacc
      361 aaggaggcga agcaggccga gaacctgcgc aagctgctgc tggcgatcgc cgatgatgtc
      421 cgggtgctgc tggtcaagct cgccgaccgc ctgcacaaca tgcgcaccct gtcccacatg
      481 ccgccagagg cccgacggcg cagcgccgag gaaaccctcg acatctacgc tccgctcgcc
      541 ggccgcatgg gcatgcatga gatgcgggag gagctcgagg acctggcgtt ccacgagctc
      601 aatcccgaag cctatacggt cgtcagcgaa cgcctcgacg cgctcgaggc gaaaaacgtc
      661 ggcctggtga ccgagatcga gcaacagctc aagcgaaagc tgctggaccg cggcatcgat
      721 gccgaagtgt cggggcggcg caagcgcgcc tattccatct ggcgcaagat ggagcgcaaa
      781 tcgatcggtt tcgagcagct ctccgacatc ttcggcttcc gcgtcatcgt gaagacgctg
      841 cccgagtgct accaggcgct cgggatcgtt catacgactt ggccggtggt gccgggccgc
      901 ttcaaggatt acgtctcgac cccgaagcag aatgattacc gctccattca caccaccgtg
      961 atcggcccga gccagcagcg cgtcgaactg cagatccgta ccaaggagat gcaccagatc
     1021 gcggactacg ggatcgccgc gcacgcgctg tacaaggacg acgtcgactc gccgacggag
     1081 ctgctgtcgc gcgaatccag cgcctatgcc tggctgcggc ggaccatcga gctgctcgcc
     1141 gagggctcca atccggaaga gttcctcgag cacaccaagc tcgagctgtt tcacgaccag
     1201 gtgttctgct tcacccccaa gggcaagctg atcgcgctgc cgcggcatgc gaacctcatc
     1261 gactttgcct atgcggtgca caccgacgtg ggcaacatgg cggtcggcgg caagatcaac
     1321 ggcaagatcg cgccgctggt gtccgagctc accaacgggg acgaggtcga gatcatcacc
     1381 tcaagggcgc agcacgcacc tccggcggcc tgggaatccc tggtgaccac cggcaaggcg
     1441 cgcgctgccg ttcgccgcgc cacccgcgtc gcggtgcgca accaatatgc cgggcttggg
     1501 cggcgcattg tcgagcgcct gtgccagcgc gcgaagatcg cctattccga cgaaaagctg
     1561 caaggcgcgc tgccgcggct ggcgcgcgcg tcgatcgaag aggtgctgtc ctcggtcggg
     1621 cgcggcgaga tgcgtgcagc cgacgtcgtg cgtgccatgt atcccgatta caaggaggag
     1681 cgcgctgcca cgatcgcgca gc
//
LOCUS       DATANS010000558         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_111869, whole genome shotgun sequence.
ACCESSION   DATANS010000558 DATANS010000000
VERSION     DATANS010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111869"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VHT22_08195"
     CDS             <1..824
                     /locus_tag="VHT22_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX3137575.1"
                     /translation="IVYGVAANVSISHLFIAGVIPGLLLASLFSGYIVVWALLHREQV
                     PIIAERTTIAQKLHASRHLIPIVLLIALVLGSIYTGIATATEAAAFGVVGALLISAVQ
                     GSLSRATFGASLMGATRLYCMIALILSGAAFLTLAMGYIGLPRHLAEWIGGLGLTRLE
                     LIVALMIFYIILGCFLDGISMVVLTMGVILPTVEKAGIDLVWFGIFIVLVVEMAQITP
                     PVGFNLFVLQGMTQKQITWIARQALPMFFLLIAATLLIYFVPQLVGYLPQQMKFR"
     gene            841..1335
                     /locus_tag="VHT22_08200"
     CDS             841..1335
                     /locus_tag="VHT22_08200"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX3137576.1"
                     /translation="MRAIVSHDYAAKLDAPFAVLGIRTSGGAVTGIEYLPKDERATAP
                     ANALAERACRQLQRYFADPGFHFSLPLAPAGSAFRRKVWDALAGIPVGESRTYGEVAR
                     RLHTAPRAVGGACGANPIALVIPCHRVVGSQGSLGGFMGTTDGDPIAIKRWLLTHEGY
                     RFGK"
     gene            1340..>2115
                     /locus_tag="VHT22_08205"
     CDS             1340..>2115
                     /locus_tag="VHT22_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX3137577.1"
                     /translation="MQTSAELIDAFCDQVWLQDGLAASSLASYRRDLSAWSAWLERHA
                     HRSLLAAERTDVEAFLAAQFQSKAKIASINRRLSTLRRFYRLQAAQATIAADPTLRVK
                     SPKLPRSLPRNLSEDQVAALLGAPDTETPLGLRDRAMLETLYATGLRVSELVGLKLSQ
                     VSLDMGVVRVLGKGNKERLVPLGEEAIDWLKRYCTTARTELGGDAKAEAVFVTARRGP
                     MSRQAFWQLIKRHAATAGIARAALSPHTLRHAFATHLLNHG"
BASE COUNT          319 a          701 c          691 g          404 t
ORIGIN      
        1 tgatcgtcta cggcgtcgcc gccaacgtct cgatctccca cctctttatc gccggcgtga
       61 tacccggtct gttgctggct tcgctctttt ccgggtacat cgtcgtctgg gcgctcttgc
      121 atcgggagca ggtgccgatc atcgccgaac ggacgacgat cgcacagaag ctccacgcgt
      181 cgcggcacct gattccgatc gttctcctga tcgcgctggt gctgggctcg atctacacgg
      241 gcatcgcgac cgcgaccgag gccgccgcgt tcggcgtcgt cggcgcgctg ctgatttccg
      301 ccgtacaggg atcgcttagt cgcgccacgt tcggcgccag cctgatggga gcgactcgcc
      361 tgtactgcat gatcgcgctg atcctttcgg gtgccgcgtt cctgaccttg gcgatgggtt
      421 acatcgggct gccgcggcac cttgccgagt ggatcggcgg tctcggcctc accaggctag
      481 agctgatcgt cgcattgatg atcttttaca tcatcctcgg ctgcttcctc gatggcattt
      541 cgatggtcgt gctgacgatg ggcgtgatat tgccgacggt cgaaaaggcg gggatcgacc
      601 tggtctggtt tggcatcttt atcgtgctgg ttgtcgagat ggcgcagatc accccaccgg
      661 tcggcttcaa cctgttcgtg ctgcagggga tgacgcaaaa gcagatcacc tggatcgcac
      721 gccaggcgct gccgatgttc ttcctgttga tcgcggcgac gctgctgatc tactttgtcc
      781 cgcagctggt aggctatctg ccgcagcaga tgaagtttcg ttgacgatgg ctaccggcgt
      841 atgcgtgcga tcgtaagtca cgactatgcg gccaagctcg acgcgccgtt cgcggtgctc
      901 ggtatccgca cgtccggcgg cgcggtcacc ggcatcgaat acctgccgaa ggatgagcgc
      961 gcgacggcgc ccgccaatgc gctcgccgag cgagcgtgcc gccagctcca gcgctatttc
     1021 gccgatcccg gattccattt cagcttgccg ctcgcgcctg ctggcagcgc gttccggcgc
     1081 aaggtgtggg acgcactggc gggcattccg gtcggcgagt cgcgcaccta cggcgaggtg
     1141 gcccgcaggc tccatacggc gccgcgcgcg gtgggcggag cctgtggcgc aaaccccatc
     1201 gctctggtca ttccctgcca tcgcgtcgtc ggcagccagg gctcgctcgg cggcttcatg
     1261 ggaacgaccg acggcgaccc gatcgcgatc aagcgatggc tgctgacgca cgaaggttat
     1321 cgcttcggaa agtaagcgga tgcagacctc cgccgagctg atcgacgcgt tctgcgacca
     1381 ggtctggctg caggacgggc tcgccgcgtc gagcctcgct agctaccggc gcgatctcag
     1441 cgcctggtcg gcgtggctcg aacgccacgc gcacaggagc ctgcttgcgg ccgagcgcac
     1501 cgacgtcgag gctttcctcg ccgcgcaatt ccagagcaag gcgaagatcg cctcgatcaa
     1561 tcggcggctg tcgaccttgc gccgctttta tcggctgcag gcggcgcagg caacaatagc
     1621 cgccgacccg accttgcgcg tcaagtcgcc gaagctgccg cgcagcctgc cgcgcaacct
     1681 gtccgaggat caggtggccg cgctgctcgg cgcgccggat acggagacgc ctctcggctt
     1741 gcgcgaccgt gcgatgctcg agacactcta cgccacgggg ttgcgcgtct ccgagctggt
     1801 cgggctcaag ctttcgcagg tgagcctcga catgggcgtc gtgcgcgtgc tgggcaaggg
     1861 caacaaggag cggctggtcc cgctcggcga agaggcgatc gactggctga agcgctactg
     1921 cacgaccgcg cgcaccgagc tcggcggtga tgcgaaggcg gaggcggtat tcgtcaccgc
     1981 gcgccgaggc cctatgtcgc gccaggcgtt ctggcaattg atcaagcgcc acgccgccac
     2041 tgccggcatc gcgcgcgccg cgctgtcgcc gcatacactg cgccacgcgt tcgccacgca
     2101 tcttctgaac cacgg
//
LOCUS       DATANS010000559         3302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_154516, whole genome shotgun sequence.
ACCESSION   DATANS010000559 DATANS010000000
VERSION     DATANS010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154516"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2223
                     /locus_tag="VHT22_08210"
     CDS             <1..2223
                     /locus_tag="VHT22_08210"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:NF002837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX3137578.1"
                     /translation="TIAECETEMAGDITIRTSLLEHRQIAGSRALYQRFQRSIAATID
                     ARAFYEAKVLEQQQRHLRYQDTAYNLEPNVKESPGGLRDLQTIVWIARAAGLGRSWHD
                     FAARGLMTGPEAREVVRQERLLCDLRIRLHYLAGRREDRLVFDVQTALARDFGLADTA
                     ERRASELLMQRYYRAAKSIRQISVVLLQNVHSRLFPAELAARPIDADFQAVDELLDVV
                     DEQLFARRPAAIFDSFLTLQQHPELKGMSARTLRALWRNRNRVDAAFRRDPANRARFI
                     DILRQPRGVTHELRRMNQYGILGRYLPVFGRIVGQMQHDLFHVYTVDEHILMVIRNLR
                     RFTEAQHAHEYPICSRLIADFARPELLYIAGLFHDIAKGRGGDHSTLGSTDARRFCRA
                     HQLSREDTELVAWLVEQHLMMSATAQKQDIADPAVVAAFAGRVGTARRLAALYLLTVA
                     DIRGTSPRVWNAWKAKLLEGLYHAASAVLDGGSARKRAPDSLETRRAEAARQLRLYAV
                     PEGAEDSLWQRLDTVYFQRHSAGDIAWHARQLYYRVEGKQPVVKARLARDGAGLQLMV
                     YLPDQKELFARICGFLGRAQLSILEAKIHTTRHGYALDTFVVHDPANPNAGYREAISY
                     IEFELQRVLSDRLPLELPAEGRVSRHLRHFPLSPEVSIFADDKGTHFILEIVAGDRPG
                     LLARIAYTLAKANVNVESAKINTLGERAEDVFLLDGARLHDEAARLRLETALYEQLRV
                     "
     gene            complement(2240..2770)
                     /gene="def"
                     /locus_tag="VHT22_08215"
     CDS             complement(2240..2770)
                     /gene="def"
                     /locus_tag="VHT22_08215"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX3137579.1"
                     /translation="MIRDILRMGDPRLLQRSTEVGRFGTPELETLVSDMRETMIAATG
                     AGLAAPQIGVGLRVVIFGFEHNDRYPDAEPVPYTELVNPVLTPLSDETEEGWEGCLSV
                     PGLRGRVRRYTRLRYKGFDPQGRPIEREANGFHARVVQHECDHLDGILYPMRMEDMRM
                     FGYTDILFPDADPRDE"
     gene            complement(2767..>3302)
                     /locus_tag="VHT22_08220"
     CDS             complement(2767..>3302)
                     /locus_tag="VHT22_08220"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HEX3137580.1"
                     /translation="LKNRGVGTILAVASVGGIAPAYRPGDLVLPHQLLDYTSGRETTF
                     FDGSDRVVIHVDFTEPYSLALRVHCLAAATAGGIPLRDGGVYAAVSGPRLETAAEIDR
                     LERDGATMVGMTGMPEAALARELGVDYAAIAVVVNQAAGRGASAHAISMASIAEVLET
                     AMDQVRALIDRVVERVP"
BASE COUNT          563 a         1114 c         1079 g          546 t
ORIGIN      
        1 acgattgccg agtgcgagac cgagatggcc ggcgacatta cgatacgcac cagcttgctc
       61 gaacatcggc agatcgccgg gtcgcgcgcg ctctaccagc gctttcagcg cagcatcgcg
      121 gcaaccatcg acgctcgcgc cttttacgaa gcgaaggtgc tcgagcagca gcagcgccac
      181 cttcgctacc aggacaccgc ctacaatctc gagcctaacg tcaaggaaag cccggggggt
      241 ctgcgcgatc tgcagacgat cgtgtggatc gcgcgcgccg cgggtctcgg tcgatcgtgg
      301 cacgactttg ccgcgcgagg cctgatgacg ggcccggagg cgcgcgaggt cgtccggcag
      361 gagcggctgc tgtgcgacct gcgcatccgc ctgcactatc ttgccggtcg tcgcgaggat
      421 cggctggtgt tcgatgtgca gaccgcactc gcccgcgact tcggcctggc cgacactgcc
      481 gagcgacgcg cgtccgaact gctgatgcag cgctattacc gggcggccaa gtcgattcgc
      541 cagatcagcg tcgtgctgtt gcagaacgtg cattcccggc tgttccccgc cgagctcgcc
      601 gcgcggccga tcgacgcgga ttttcaggcc gtcgacgagc tgctcgacgt cgtcgacgaa
      661 cagttgttcg ctcgccggcc ggccgcgata ttcgacagct ttctcacgct gcagcagcat
      721 ccggagctca agggaatgtc ggcccgcacg ttgcgcgcgc tgtggcgaaa ccgcaatcgc
      781 gtcgatgccg cctttcgccg cgacccggcg aatcgggcgc ggttcatcga catcctccgc
      841 cagccgcgcg gcgtcacgca cgagctgcgc cggatgaacc agtacggcat cctggggcgc
      901 tatctgccgg tgttcggccg gatcgtcggc cagatgcagc acgacctgtt ccacgtctac
      961 accgtcgacg agcacatcct gatggtgatc cgcaatctgc gccgattcac cgaggcacaa
     1021 cacgcgcacg agtatccgat ttgctcgcga ctgatcgccg acttcgcgcg gccggaattg
     1081 ctctatatcg ccggactctt tcacgatatc gccaagggcc ggggcggcga tcattcgacg
     1141 ctgggcagca ctgacgcgcg gcgattctgc cgcgcccacc agctttcgcg cgaagatacc
     1201 gagctcgtcg cgtggctggt cgagcagcac ctgatgatgt cggcaactgc acaaaagcag
     1261 gacattgccg atccggcggt cgtcgcagca ttcgccggca gggtcggaac cgcccggcgg
     1321 ctagcggcgt tgtatctgct gacggtggcc gacatccgcg gcaccagccc gcgggtttgg
     1381 aacgcctgga aggcaaagct gctcgagggc ctgtaccacg cggccagcgc ggtgctggac
     1441 ggaggcagcg cgcgcaagcg cgcgcccgac agcctggaaa cgcggcgcgc cgaggccgcg
     1501 cgccagttgc gcctttatgc ggttccggaa ggcgccgaag acagcctgtg gcagcgcctg
     1561 gacactgtct atttccagcg gcacagcgcc ggcgacatcg cctggcatgc gcggcagctt
     1621 tattaccggg tggaaggcaa gcagcccgtg gtcaaggcga gactcgcgcg cgacggcgcc
     1681 gggttgcagc tgatggtcta cctgcccgac cagaaggagc tgtttgcgcg catttgtgga
     1741 tttctcggcc gggcccagct gtcgattctc gaagccaaga ttcatacgac gcggcacggc
     1801 tatgcgctcg acacctttgt cgtgcacgac ccggcgaacc ccaacgccgg ttatcgcgag
     1861 gcgatcagct acatcgaatt cgagctgcag cgggtgctga gcgatcggct gccgctcgag
     1921 ctgccggcgg aaggcagggt cagccgccac ctcaggcatt ttcccctgtc gcccgaagtc
     1981 agcatctttg ccgacgacaa gggaacgcac ttcattctcg agatcgttgc cggcgaccgg
     2041 ccggggctgc tggcgcgcat tgcctatacg ctcgccaagg cgaacgtcaa cgtcgaaagc
     2101 gccaaaatca atacgctggg cgagcgcgcg gaggatgtct tcctgctcga cggcgcccgt
     2161 ctgcacgacg aggcggcgcg gctgaggctc gagacagcgc tctacgagca gctgcgggtg
     2221 tagcgcacgc cggccggctt cactcgtcgc ggggatcggc gtcgggaaac aggatgtcgg
     2281 tatagccgaa catgcgcatg tcttccatgc gcatcggata caggatgccg tccaggtggt
     2341 cgcactcgtg ctggaccaca cgcgcgtgga agccgttcgc ctcgcgttcg atcggcctgc
     2401 cctgcggatc gaagcccttg tagcgcaggc gcgtgtagcg gcgcacgcgg ccgcgcagac
     2461 ccggcaccga caggcagccc tcccatcctt cctcggtctc gtcggaaagc ggcgtcagca
     2521 ccggattgac cagctcggtg tagggcaccg gctcggcatc cggatagcgg tcgttgtgct
     2581 cgaatccgaa aatgacgacg cgcagtccga caccgatctg cggcgctgcg aggccggcgc
     2641 cggtcgccgc aatcatcgtt tcgcgcatat cggacacgag cgtctcgagc tcgggggtgc
     2701 cgaagcgtcc gacctcggtc gaacgctgaa gcagcctcgg atctcccatg cggagaatgt
     2761 ctcgaatcac ggcacgcgct ccacgacccg gtcgatcagc gccctgactt ggtccatcgc
     2821 ggtctccagg acttctgcaa tgctcgccat cgaaatcgcg tgggcgctcg ccccacggcc
     2881 cgcggcttgg ttgacgacga ccgcaatggc cgcgtagtcg acgccgagct cgcgcgccag
     2941 cgcggcttcc ggcatccccg tcataccgac catcgtcgcg ccatcgcgtt ccaggcgatc
     3001 gatctcggcg gcggtttcga gcctcgggcc ggataccgcg gcatagacgc cgccgtcgcg
     3061 cagcgggatc cctccagccg tcgcggcggc gagacaatgg acccgcagcg cgagcgaata
     3121 cggctccgtg aaatcgacat ggatcacgac ccggtcgctg ccatcgaaga acgtcgtttc
     3181 ccgcccgctg gtgtaatcga gcaactgatg cggcagaacc aggtcgcccg gccggtacgc
     3241 cggggcgatg ccgccgaccg aggcgacagc gagtatcgtg ccgacgccac ggttcttgag
     3301 cg
//
LOCUS       DATANS010000560         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_532084, whole genome shotgun sequence.
ACCESSION   DATANS010000560 DATANS010000000
VERSION     DATANS010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532084"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..>1921
                     /gene="putA"
                     /locus_tag="VHT22_08225"
     CDS             28..>1921
                     /gene="putA"
                     /locus_tag="VHT22_08225"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEX3137581.1"
                     /translation="MLTELVPSLTALAKIAKSHGIGMTIDAEEAERLELSLEIFARLR
                     RDPALAGWEGLGLAVQAYQKRALAVIDWLIALGTECRMRIPVRLVKGAYWDTEIKRAQ
                     VLGLDSYPVFTRKAHTDVSYLACVQRMLDSAGKNGALFPMFATHNAHSAAAIVERATH
                     GGIAPDAFEFQRLHGMGEALYDEIVSADKLDIACRVYAPVGSHQDLLPYLVRRLLENG
                     ANTSFVNRIGDTTVSIADIVADPAAKVRAQGGAPNPALPLPGQLFAPERRNSQGLSLA
                     DQPAMAALDAAFATRAQTRWTAAPTIARQATRRAREVVDPSDHRRAIGTVADADLADV
                     DTALSRAAAAQPTWDAVGGDGRADVLERAADGIEGAREELVVLLAREAGKNRADAIGE
                     VREAADFCRYYAMLARRHFARPQSLPSPTGESNELALHGRGVFACISPWNFPLAIHVG
                     QIAAALAAGNAVIAKPAEQTPLLAQCAAGILAAAGVPAGVYAMLPGAGETVGAALVDD
                     PRTAGVAFTGSTEVARAIALALARRTTLVPLIAETGGQNALIVDSSALPEQLVVDAVT
                     SAFNSAGQRCSALRVLFLQQEVAPRIVELLIGAMNELAIGDPASLATDVGPVIDDEAR
                     KTLETHV"
BASE COUNT          303 a          664 c          670 g          284 t
ORIGIN      
        1 cgctacgagc atccgcaaca tcgacgcgtg ctcaccgagc ttgtcccgtc gctgacggcg
       61 ctcgcgaaaa tcgccaagag ccacggcatc ggcatgacca tcgatgcgga agaagccgaa
      121 cggctcgagc tttcgctgga aatcttcgcg cggctgcggc gcgatccggc gctcgccggc
      181 tgggagggcc tgggccttgc cgtgcaggcg tatcaaaagc gcgcgctggc ggtgatcgac
      241 tggctgatcg cgctgggcac cgaatgccgc atgcgcattc cggtgcggct ggtcaagggc
      301 gcgtattggg acacggagat caagcgcgcg caggtcctgg gcctcgactc ctatccggtg
      361 ttcacgcgca aggcgcacac cgacgtctcg tacctcgcct gtgtccagcg catgctcgac
      421 agcgccggca agaacggcgc gctgtttccg atgttcgcca cccacaacgc gcacagtgcc
      481 gcggcgatcg tcgagcgggc cacgcatggc ggcatcgcgc cggatgcgtt cgaattccag
      541 cgcctgcacg gcatgggcga ggcgctgtac gacgagatcg tgtccgccga caagctcgat
      601 atcgcctgcc gcgtctacgc gccggtcggc agccaccagg atttgctgcc ctacctcgtc
      661 cggcggctgc tcgaaaacgg cgccaatact tcattcgtca atcgcatcgg cgacacgacg
      721 gtgtcgatcg ccgatatcgt tgccgacccg gcggcaaaag ttcgcgcgca gggcggtgcg
      781 ccgaatcctg cgttgccgtt gcctggccag ctgttcgcgc ccgagcgccg caattcgcag
      841 ggcttgagcc tcgccgacca gccggcgatg gcggcgctgg acgcggcatt cgcgacgcgg
      901 gcgcaaacac gatggaccgc ggcgccgacc atcgcacggc aagcaacgcg acgcgcgcgc
      961 gaagtcgtcg atccgtccga ccatcgccgt gcgataggga ccgtcgccga cgccgatctc
     1021 gccgacgtcg acacggcgct atcgcgggcg gcggccgcgc agccgacgtg ggacgcggtc
     1081 ggcggcgatg gccgcgccga cgtgctcgag cgcgcggccg acggcatcga aggcgcgcgc
     1141 gaggagttag tcgtgctgct tgcgcgcgaa gcaggaaaga accgcgccga tgcgatcggc
     1201 gaagtgcgcg aggcggccga tttctgccgc tactacgcga tgctcgcccg ccggcacttc
     1261 gcgaggccgc agtcgctgcc gtcgccgacc ggcgaatcga acgagcttgc gttgcacggc
     1321 cgcggcgtgt tcgcgtgcat cagcccgtgg aattttccat tggcgatcca tgtcggccag
     1381 atcgccgcgg cgctcgccgc cggcaatgcg gtcatcgcca agcccgccga gcagacgccg
     1441 ttgctcgcgc agtgcgcagc cgggatcctg gccgcggccg gcgtgccggc cggcgtctac
     1501 gcaatgctgc ccggcgcggg tgaaaccgtc ggcgccgcgc tggtcgacga tccacgcacc
     1561 gccggcgtgg cgttcaccgg atcgaccgag gtcgcgcgcg cgatcgccct cgcgctggcg
     1621 cggcgaacga cgctggtgcc gctgatcgcc gaaaccggcg gacagaacgc gctgatcgtc
     1681 gacagctccg cgctgccgga gcagctggtt gtcgacgcgg tgacgtcggc attcaacagc
     1741 gccggacagc gctgctcggc gttgcgcgtg ctgttcctgc agcaggaagt ggcgccccgc
     1801 atcgtcgaac tgctgatcgg cgcgatgaat gagctcgcga tcggcgatcc ggcatcgctc
     1861 gcgaccgacg tcggcccggt gatcgacgac gaagcgagga agacgctgga gacgcacgtc
     1921 g
//
LOCUS       DATANS010000561         5589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_783668, whole genome shotgun sequence.
ACCESSION   DATANS010000561 DATANS010000000
VERSION     DATANS010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783668"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..366
                     /locus_tag="VHT22_08230"
     CDS             154..366
                     /locus_tag="VHT22_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEX3137582.1"
                     /translation="MNQPDKRRRGARCPQCGKTAALTSDNPFRPFCSERCKLIDLGAW
                     AAESYRVPVAEDPADPDADQAKSERS"
     gene            complement(387..1343)
                     /locus_tag="VHT22_08235"
     CDS             complement(387..1343)
                     /locus_tag="VHT22_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HEX3137583.1"
                     /translation="MSERTIVRVAAAILHRGDGKVLLAQRLPGTPYPDYWEFPGGKLE
                     PGESARDALLRELEEELGIVVTRAAPWLTQRYDYPHAHVQLEFFRVFEWQGEPHGRDG
                     QAIAWQQPTAIEVAPLLPANATVLRALALPAVYAISMAEDLGEAAFLARARVAFDAGL
                     KLVQLREKSFAPERLRALADELLAISAPYGARVLLNGDANTARELAFAGVHWSAARLL
                     AAPARPDDMLCAASTHDATELAQAAKLGVDFVVLGPVSETPTHPNARPLGWQRFAELA
                     AGSPMPVYALGGLSPADLDIAIAHGAHGVALRRAAWPALPRS"
     gene            complement(1340..2230)
                     /locus_tag="VHT22_08240"
     CDS             complement(1340..2230)
                     /locus_tag="VHT22_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3137584.1"
                     /translation="MARAESLLGRLEALLPAAPAPPDWKARAFRWRKRGQRGYLQSVS
                     RPHAISLDDLVAIDAQKAAIDANTRQFVAGLPANNVLLTGSRGTGKSSLVKAMLGKYA
                     AKGLRLIEVDKGDLVDLPDIVEPIAERPERFIVFCDDLSFDANEPGYKALKVMLDGTI
                     AAASSNVLIYATSNRRHLLPEYFSENLETRHIGEEVHPGESVEEKISLSERFGVWISF
                     YPFTQDDYFAAVHQWLRFYGVPVPDEGAALERLHREAVQFALQRGSRSGRIAWQFARA
                     QAGARALDGGKGKGRNKSTK"
     gene            complement(2264..3493)
                     /gene="argJ"
                     /locus_tag="VHT22_08245"
     CDS             complement(2264..3493)
                     /gene="argJ"
                     /locus_tag="VHT22_08245"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX3137585.1"
                     /translation="MPVNYHPPTPEQLLPVAGVTLGTAAGRIKNWSRDDVILVSLDQG
                     SRASGVFTRNRFCAAPVIVCREHLANGQVTRALLINAGNANAGTGQRGVDDARTCCAA
                     VARALDCAEYEVLPFSTGVIMERLPVERVVAAVPKCVAALSPSGWDAAMRAIMTTDTV
                     PKAASCRLDIDGVPVTITGIAKGAGMIAPNMATMLGFVATDAPVEGLLLGELAASLAA
                     ASFNRITIDGDTSTNDSFVLLATGKAAMAPIVRSDDPRLRSLHDALELVAVQLAQAIV
                     RDGEGATKFITIVVDGGRDDGECDRVARAIADSPLVKTAFFASDPNLGRIVCAIGNAA
                     VEDLDPGCVSFWLDDVLVVADGGRAEGYREEDGKRVMEQAEIGIRVVLGRGGATATVW
                     TCDLSHGYVTINADYRS"
     gene            complement(3603..>5589)
                     /gene="secA"
                     /locus_tag="VHT22_08250"
     CDS             complement(3603..>5589)
                     /gene="secA"
                     /locus_tag="VHT22_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX3137586.1"
                     /translation="NSIIPQLVRGEELEENKKTGDYVVDERNHSAVLTEDGVDKAERL
                     LGVGNLYDPDNMELLHCVEQALKAHTLYRLDHQYVVQDGEVIIVDDFTGRLMKGRRWS
                     DGLHQAVEAKEGVSVQRENQTLASITFQNYFRMYTKLAGMTGTADTEAFEFQQIYHLE
                     TVVIPTNVAMIRKDENDLVFRTFQEKVDAIIADIKDCYQRGQPVLVGTTSIENSELLA
                     QFLVKEKLPHEVLNAKQHAREAVIVAQAGRPKAITIATNMAGRGTDIVLGGAIEPTLL
                     AIREDESRAQTDRDLEVAKLRREWQVLHDQVIAAGGLHIVGTERHESRRIDNQLRGRS
                     GRQGDPGSSRFYLSLEDPLLRIFGGERLSGIMQRLKMPEGEPIENPIVNRSIAKAQNR
                     VESRNFDIRKQLLEYDDVANDQRRVIYQQRNELLETEDISETIRNMIAGQIEDTVRQY
                     VPPESVEEQWDVPALESALAGEYQLRIPAGEWLKNEPELSDEALRERAREFATKQYEQ
                     KLGLVGPELFHQFERNLMLQTLDQHWREHLGSLDHLRQGIHLRGYAQKNPKQEYKRES
                     FELFSDMLDRIKHDVVKIVLTVQVRSEQDMQAVEEAPAVSNVQYQHTDYDTALAAPPD
                     QPPAALPPFTRAGQKVGRNDPCPCGSGKKFKHCHGAI"
BASE COUNT          883 a         1885 c         1824 g          997 t
ORIGIN      
        1 tgatcgtggc cgacggtttc agctgccggc accagatcag ggacggtgca cagcgcgatg
       61 cgctgcacgt ggcgagcgtg ctggcgatgg cgctggacta gcgcggcgag cgcggactgg
      121 cgcattgcgc gcggttcgcg caaaattatc ggcatgaacc agcccgataa acgccggcgc
      181 ggcgcacgct gcccgcaatg cggcaagacc gccgctctga cgtccgacaa tccatttcgc
      241 cccttctgct cggaacgctg caagctgatc gatctcgggg cctgggcagc cgagtcgtat
      301 cgcgtgccgg tcgcggaaga cccggccgat cctgacgccg accaggcgaa gtcggagcgg
      361 tcgtagccag tcctagctag tcgtacctag ctgcgcggaa gcgccggcca cgcggcgcgc
      421 cgcagcgcga caccgtgcgc gccgtgcgcg atcgcgatgt cgagatcggc gggcgagagc
      481 ccgcccagcg cgtacaccgg catcggcgat ccggcagcga gctcggcgaa acgctgccag
      541 cccagcggac gcgcgttggg atgcgtcggc gtctcggaca ccgggccgag cacgacaaag
      601 tcgacgccga gcttcgccgc ctgcgcgagc tcggtcgcgt cgtgtgtcga cgcagcgcat
      661 agcatatcgt cgggacgcgc cggcgccgcg agcaggcgcg ccgcactcca gtgcactcct
      721 gcgaaagcga gctcgcgcgc ggtgttcgcg tcgccgttga gcagcacgcg tgcgccgtac
      781 ggcgcactga tcgccagcag ctcgtcggca agcgcgcgca ggcgttccgg cgcaaacgat
      841 ttctcccgca gctgaaccag cttcagccct gcgtcgaatg cgacgcgcgc ccgcgcgaga
      901 aacgccgcct cgcccagatc ctcggccatc gagatcgcat acacggccgg caaggcgagc
      961 gcgcgcagca ccgtcgcatt ggccggcaac agcggtgcca cctcgatcgc ggtcggctgt
     1021 tgccatgcga tcgcctggcc gtcgcggcca tgcggctcgc cctgccattc gaacacccgg
     1081 aagaactcga gttggacgtg tgcgtgcgga tagtcatagc gctgggtgag ccacggggcg
     1141 gcccgcgtca cgacgatgcc gagctcctcc tcgagctcgc gcagcagcgc gtcgcgggcc
     1201 gattcgcccg gctcgagctt gccaccggga aattcccagt aatccggata cggcgttccg
     1261 ggcagacgtt gcgccagcag caccttgccg tcgccccggt gcagtatcgc cgcggcgacg
     1321 cgcacgatcg tgcgctcgct cacttggtgc tcttgttcct gcccttgccc ttgccgccgt
     1381 cgagcgcgcg ggcgccggcc tgcgcccgcg cgaactgcca ggcaatgcgg ccgctgcgcg
     1441 atccgcgctg cagcgcgaat tgcaccgctt cgcgatgcag gcgttccagc gcagcgccct
     1501 cgtccggcac cggcacgccg tagaagcgca gccattggtg gaccgcggca aagtagtcgt
     1561 cctgagtgaa cggatagaac gagatccaca cgccgaagcg ttccgacagc gatatctttt
     1621 cctcgaccga ttcgccggga tgcacttctt cgccgatgtg ccttgtctcg aggttctcgc
     1681 tgaaatactc gggcagcaga tgccgccggt tcgacgtcgc atagatcagc acgttcgacg
     1741 aagccgccgc gatcgtgccg tcgagcatga ccttgagcgc cttatagccc ggctcgttgg
     1801 cgtcgaacga caagtcgtcg caaaacacga tgaaccgctc gggccgctcg gcgatcggct
     1861 cgacaatgtc cggcaggtcg accaggtcgc ccttgtcgac ttcgatcagg cgcaggccct
     1921 tcgccgcgta cttgcccagc atcgctttca ccagcgacga cttgcccgtg ccgcgcgatc
     1981 cggtgaggag cacgttgttc gccggcaggc ccgcgacgaa ctgccgggta ttggcgtcga
     2041 tcgcggcctt ttgcgcatcg atcgccacca ggtcgtcgag gctgatcgca tgcggacgcg
     2101 acaccgactg caggtagccg cgctgtccac gcttgcgcca gcgaaacgcc cgcgccttcc
     2161 agtccggcgg cgccggtgcg gcgggaagca gcgcctcgag gcggcccaac aggctttcgg
     2221 cgcgcgccat caggcggtcg aggtcgcgtt cgttcacgat tcgctagctc cggtaatcgg
     2281 cgttgatcgt gacgtagccg tgcgacagat cgcaggtcca taccgtcgcc gtcgcgccgc
     2341 ctcgcccgag cactacgcga atgccgatct cggcctgttc catcacgcgc ttgccgtctt
     2401 cctcgcgata gccttcggcg cggccgccgt ccgcgacgac gagcacgtcg tcgagccaga
     2461 acgacacgca gcccgggtcg agatcctcca ccgccgcgtt gccgatcgcg cagacgatac
     2521 ggcccagatt gggatcggag gcgaaaaacg ccgtcttgac cagcggcgaa tcggcgatcg
     2581 cgcgcgcgac tcgatcgcat tcgccgtcgt cgcgtccacc gtcgacgacg atggtgatga
     2641 atttggtcgc tccctcgccg tcgcgcacga tcgcctgcgc cagctgcacg gcgacgagct
     2701 ccagcgcatc gtgcaaggac cgcaggcggg gatcgtcgga tcgaacgatc ggcgccatcg
     2761 ccgccttgcc ggtggccaag aggacgaagc tgtcgttggt cgacgtatca ccgtcgatgg
     2821 tgatgcgatt gaacgacgcg gcggcgaggc ttgccgcgag ctcgccgagc agcaagcctt
     2881 ccaccggcgc gtcggtggcg acgaagccca gcatcgtcgc catgttgggt gcgatcatgc
     2941 ccgcgccctt ggctatgccg gttatggtga cgggcacgcc gtcgatgtcg aggcggcacg
     3001 atgccgcttt gggcactgtg tcggtagtca tgatcgcgcg catcgccgcg tcccatcccg
     3061 aaggcgacag cgcggccacg catttcggca ccgcagccac gacccgctcg accggcaagc
     3121 gctccatgat gacgccggtc gagaaaggca ggacttcgta ttcggcgcaa tccagtgcgc
     3181 gcgccacggc ggcgcagcag gtgcgcgcat cgtcgacacc gcgctggcct gttcccgcat
     3241 tggcgttgcc ggcgttgatc agcagcgcgc gcgtgacctg tccgttcgcc aggtgctcgc
     3301 ggcagacgat caccggggcg gcgcaaaacc ggttccgggt gaacacgccc gaggcgcgcg
     3361 atccctgatc gagacttacc aggatgacgt cgtcgcgcga ccagttcttg atgcgaccgg
     3421 cggcggtgcc caaagtgacg ccggcgaccg gcaacagctg ctcgggcgta ggcggatggt
     3481 agttgaccgg catgggtgcg cgagcagaag cgtcggtcgg attctaccgc agcgttcgcg
     3541 ggcgccgcag ggcggccgcg tcatggacta tcgagccttg ggccggcgcg cgggctgggc
     3601 cgctagatcg cgccgtggca gtgcttgaac ttcttgcccg atccgcaggg acacggatcg
     3661 ttgcggccga ctttctgtcc ggcgcgcgtg aacggcggca gcgccgccgg aggctggtcg
     3721 ggcggcgcgg ccagcgccgt atcgtagtcg gtgtgctggt attgcacatt ggagaccgct
     3781 ggcgcctcct cgactgcctg catgtcctgc tcggagcgaa cctgcacggt cagcacgatc
     3841 ttgaccacgt cgtgcttgat gcggtcgagc atgtcggaaa aaagctcgaa cgactcgcgc
     3901 ttgtattcct gcttggggtt cttttgcgcg tagccgcgca gatgtatccc ctgccgcaga
     3961 tgatcgagcg agcccaggtg ctcgcgccag tgctggtcga gcgtctgcag catcaggttg
     4021 cgctcgaact ggtggaagag ctctggcccg accaggccaa gcttctgctc gtattgcttg
     4081 gtcgcgaact cgcgcgcgcg ctcgcgcagc gcctcgtcgg agagctcagg ctcgttcttc
     4141 aaccactcgc ctgccgggat gcgaagctgg tactcgccgg ccagcgccga ttcgagcgcc
     4201 ggcacgtccc actgctcttc gaccgactcg ggcgggacgt actgccgcac cgtgtcctcg
     4261 atctgtccgg cgatcatgtt gcggatcgtc tccgagatgt cctcggtctc gagcagttcg
     4321 ttccgttgct ggtagatcac tcgccgctgg tcgttcgcga cgtcgtcgta ttcgagcaat
     4381 tgcttccgga tgtcgaagtt gcgcgactcg acgcggttct gcgccttggc gatcgagcgg
     4441 ttgacgatcg gattctcgat cggctcaccc tcgggcatct tcagccgctg catgatgccg
     4501 gacaggcgct cgccgccgaa gatgcgcagc aaaggatcct cgagcgacag gtagaagcgc
     4561 gacgagcccg gatcgccctg gcgcccggaa cggccgcgca actggttgtc gatgcgccgc
     4621 gattcgtggc gctcggtgcc aacgatatgc aggccgccgg cggcgatcac ctgatcgtgc
     4681 agcacctgcc attcgcggcg gagcttcgcg acctccaggt cccggtcggt ctgcgcgcgc
     4741 gactcgtctt cgcgaatcgc gagcaaggtc ggttcgatcg cgccgccgag cacgatgtcg
     4801 gtgccgcggc ctgccatgtt ggtcgcgatg gtgatcgcct tcggccgccc ggcctgcgcg
     4861 acgatcaccg cctcgcgtgc gtgctgcttg gcgttcagca cctcgtgcgg aagcttttcc
     4921 ttgaccagga attgcgcgag cagctccgaa ttctcgatcg acgtggtgcc gaccagcacc
     4981 ggctgtccgc gctggtagca gtccttgatg tcggcgatga tggcgtcaac tttttcctgg
     5041 aacgtgcgga acaccagatc gttctcgtcc ttgcggatca tcgccacgtt ggtcggtatc
     5101 acgaccgtct cgaggtggta gatctgctgg aactcgaacg cctcggtgtc cgccgtgcct
     5161 gtcatgccgg cgagtttcgt gtacatgcgg aagtagttct ggaacgtgat cgacgcgagc
     5221 gtctgatttt cccgctggac cgaaacgcct tccttcgctt ccaccgcctg gtgcaaacca
     5281 tcagaccagc ggcggccctt catcaggcgg ccggtgaagt catcgacgat gatcacctcg
     5341 ccgtcctgca caacgtactg gtggtcgagt ctgtaaagcg tgtgcgcctt caacgcctgc
     5401 tcgacacagt gaagcagctc catgttgtcc ggatcgtaga gattgccgac gcccagcagc
     5461 cgttccgctt tgtcgacacc atcttccgtc aacactgccg aatgatttct ctcatcaact
     5521 acgtaatcac ccgtcttctt attctcttca agctcttcac cgcgcacgag ctgcgggatg
     5581 attgagttc
//
LOCUS       DATANS010000562         4473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_702307, whole genome shotgun sequence.
ACCESSION   DATANS010000562 DATANS010000000
VERSION     DATANS010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702307"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VHT22_08255"
     CDS             complement(<1..1056)
                     /locus_tag="VHT22_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3137587.1"
                     /translation="MFSQFFINRPIFAAVIAIIITLAGLIASQVLPVAQYPEIAPPTI
                     VITANYPGASAETLALTVAAPIEEQLSGVENLIYFSSTAATNGTLTIACTFEVGSNAD
                     KAVIDVNNRVAIALPRLPDVVRQTGVVAQKRSTDILLVIALGSNDPRYDTLYLSNYAT
                     INLLDELKRIPGVADATIFGARDYSMRVWLNPAKMSQLGVTTTDVAQAIATQNNQYAA
                     GKIGAEPAPPGQSLVYTVTARGRLVEPEDFGNIVLRSTGPGGALRIREVARIELGAVS
                     YDAFTNLDGKPTVGIAVFLQSGGNALKVADSVRAGIARLEQSFPQGVTQVIPFDTTRF
                     VRSSIHEVIITLSEAAAL"
     gene            complement(1069..2268)
                     /locus_tag="VHT22_08260"
     CDS             complement(1069..2268)
                     /locus_tag="VHT22_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3137588.1"
                     /translation="MSFLRLLSSYAVKRCAALVVLGCLAACGKDAAAPAATVAAPIAV
                     RTLTVEPRKVPIRFDIVGQIEGSKQVEVRARVSGILQKQFYNEGDAVKDGAPLFEIDR
                     APYEIALSQARAQLAQATAQVDQARREEARLKPLVQDRAVSRKEYDDATSSRQLAEAA
                     IQQANASIRQAELNLSYTRVVAPLPGITGRAEHSIGTLITTDANGSLLTTINQVSPVW
                     VRFSLAEGDLAKLPDGRLNRATPVVMELLLPDGSQYPLKGRLNFAATAIDTRLGTQQL
                     RAEFDNPRQQLLPGQFVTVRITAGERDKVFLVPQAAVVQTEKGNLVFTVDAEGKAQAR
                     PVKTGDWIGKDWTILSGLNSGDKVVVDNLLKLQPGAPVTEAPPAAATAPAAAPTAQTA
                     PAAPGKK"
     gene            2390..3367
                     /locus_tag="VHT22_08265"
     CDS             2390..3367
                     /locus_tag="VHT22_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX3137589.1"
                     /translation="MFVRAVTATLAALLLSLSLPLCAAEQGYPSKPIRLVVPFPAGGS
                     LDAVARAIGQKLSIAWGQPVIIDNRPGAGGNIGADLVAKSAPDGYTILEGALSTHAVN
                     VSLYSKMPYDPIKDFAPITLVAVTPNVLVLNPSVPANSVAELISYAKANPGKLAFGSG
                     SNGSAGHLAGELFKTEAKVDMVHVPYKGGAPAMQDLLGGQIQLMFDNLANSMQQVRAG
                     KLKALAVTTAKRSPLVPDLPTLAEAGLPGFDIYTWWGFMAPAGTPKEIIAKWNAEVTR
                     ILNTPEMQAFFAQQGAEPTPTTPEQFMALIQGEIPKYAKIIKASGAKVD"
     gene            3412..4380
                     /locus_tag="VHT22_08270"
     CDS             3412..4380
                     /locus_tag="VHT22_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX3137590.1"
                     /translation="MRILRRAALGALALLLAGGAWAQPYPTKSIRLVVPFPAAGTTDI
                     LAREVAQRLSVSFGQSVVVDNRPGAAGNIGSDMVAKSAPDGYTLLMGTVGTHAINPSL
                     YAKMPYNHVKDFVPIVLVAGVPNVLEVTPSLPVNSVADLIKLAKEKPGQLNFASSGSG
                     TSIHLSGELFKTMTGVDMMHVPYKGSAPALTDLMGGQVQLMFDNLPSSLAQIKAGKLR
                     AIAVTSAQRSPALPNIPTIAESGLPGFEASSWFGLLAPAGTPPAIVARVNADVNEWLQ
                     TAEAKEKLLAQGAIPAGGTPEQFAAHIRVETEKWAKVVTVSGAKVD"
BASE COUNT          799 a         1455 c         1443 g          776 t
ORIGIN      
        1 cagggctgcc gcctccgaca gggtgatgat cacttcgtgg atcgacgatc ggacgaagcg
       61 ggtcgtatcg aacggaatga cctgcgtcac accctgcgga aagctctgct ccagccgggc
      121 gatgcccgcg cgcaccgaat cggcgacctt gagcgcgttg cctcccgatt gcagaaacac
      181 ggcgatgccc accgtgggct tgccgtcgag attggtgaac gcgtcgtagc tgaccgcgcc
      241 gagctcgatg cgggcgacct cgcgaatgcg cagcgccccg cccggtccgg tcgagcgcag
      301 gacgatgttg ccgaaatcct cgggctcgac cagccggccc cgtgcggtca ccgtgtacac
      361 cagcgattgc ccgggaggcg ccggctctgc gcctatcttt cccgccgcgt actggttgtt
      421 ctgcgtcgcg atcgcctgcg cgacgtcggt cgtggtgact cccagctgcg acatcttcgc
      481 aggattgagc cacacccgca tcgagtaatc gcgcgcaccg aagattgtcg cgtcggcgac
      541 tcccgggatg cgcttcagct cgtcgagcag gttgatcgtc gcgtagttgg acagataaag
      601 cgtgtcgtac cgcggatcgt tcgaacccag cgcgatcacc agcagaatgt cggtcgagcg
      661 cttttgcgcg acgacgccgg tctgccgcac gacgtcgggc agccgcggca gcgcgatcgc
      721 gacgcggttg ttgacgtcga tcaccgcctt gtcggcgttc gacccgactt cgaacgtgca
      781 agcgatcgtc agcgtgccgt tggtcgccgc cgtcgagctg aaatagatca ggttctcgac
      841 gccggagagc tgctcctcga tcggggccgc caccgtcagc gccagcgtct cggcgctcgc
      901 gcccggatag ttggcggtga tgacgatcgt cgggggagcg atttccggat attgcgcgac
      961 cggaagcact tgcgaggcga tcaatccggc cagcgtgatg atgatcgcga tgacggcggc
     1021 gaaaatcggc cggttgatga agaactgcga aaacaacgtc gcctcgcctt atttcttgcc
     1081 gggcgccgct ggcgcggttt gagcggtggg cgccgcagca ggcgcggtgg ccgccgccgg
     1141 aggcgcctcc gttaccggag cgcccggttg caatttcaga agattgtcga cgaccacctt
     1201 gtcgcccgaa ttgaggccgg acaggatcgt ccagtccttg cctatccaat cgcctgtctt
     1261 caccggtcgc gcctgcgctt tgccttcggc gtcgacggtg aaaacgagat tgcctttttc
     1321 ggtctggacg acggcggcct gcggcacgag aaacaccttg tcgcgctcgc cggcggtgat
     1381 cctgaccgtg acgaactgcc ccggcagcaa ttgctggcgc ggattatcga actcggcgcg
     1441 cagttgctgc gtgccgagcc gtgtatcgat cgcagtggcg gcaaaattga gccgcccttt
     1501 caacgggtat tgggacccgt cgggaagcag caactccatc acgaccggcg tcgcgcgatt
     1561 caggcgtccg tcaggcagct tggccaaatc cccctcggcc aggctgaagc gaacccagac
     1621 ggggctcacc tggttgattg tcgtcagcag gctgccattg gcatcggtcg tgatcagcgt
     1681 gccgatcgaa tgctctgcgc gtccggtgat gccgggcagc ggcgcgacca cgcgcgtata
     1741 cgagagattc agctcggcct gcctgatcga cgcattggcc tgttgaatcg ccgcttccgc
     1801 gagctgccgc gacgatgtcg cgtcgtcgta ttccttccgg ctcacggcac ggtcctgtac
     1861 cagaggcttc aatcgcgcct cttcgcgccg cgcctggtcg acttgcgcgg tcgcttgcgc
     1921 gagctgtgcc cgcgcctgcg agagcgcgat ttcgtacgga gcgcgatcga tctcgaatag
     1981 aggcgcgccg tccttcaccg catcgccttc gttgtagaac tgcttttgca gaatgcccga
     2041 cacacgggcg cgcacctcga cctgcttcga tccttcgatt tgtccgacga tgtcgaatcg
     2101 gatcggcacc ttgcgcggct cgacggtaag cgtgcgcacg gcgatcggcg cggctacggt
     2161 tgcggcgggc gccgcggcat ccttgccgca cgcggcgagg caacccagca ccaccagcgc
     2221 tgcacagcgt ttcactgcgt atgaagacaa caggcgcaaa aaggacatcg atacattccc
     2281 ggactgcggc atgacaggga tcgaaaacgg caaacgcgct actggctcaa gcgcgcgagc
     2341 ggacgattat atacaatcgc cgagaggcta tcggatgagg atcagaccca tgttcgttcg
     2401 cgcggttacc gctacgctgg cggcacttct tctgtcattg tcactgccgc tgtgcgctgc
     2461 ggagcaaggc tatccgtcga aaccgatccg cctggtcgtc ccgtttccgg ccggcgggtc
     2521 gctcgacgcg gtcgcccgcg cgatcggaca gaagctctcg atcgcctggg gacagccggt
     2581 gatcatcgac aaccgtccgg gcgccggcgg caacatcggc gccgacctgg tcgcgaaaag
     2641 cgcgcccgac ggctacacga tcctcgaggg cgcactgtcg acgcacgcgg tcaacgtcag
     2701 cctgtacagc aagatgccgt acgacccgat caaggacttc gcgccgatca ccctggtcgc
     2761 ggtcacgccg aacgtgctgg tgctgaatcc ttcggtgccg gccaattcgg tcgccgagct
     2821 gatcagttac gccaaggcga atccgggcaa gctcgcgttc ggctcgggca gcaacggcag
     2881 cgccggtcat cttgccggcg agctgttcaa aaccgaggcc aaggtcgaca tggtgcacgt
     2941 gccctacaag ggcggcgcgc ccgcgatgca ggatctgctc ggcggtcaga tacagctgat
     3001 gttcgacaac ctggccaatt cgatgcagca ggtccgcgcc ggcaagctca aggcgctggc
     3061 ggtcacgact gccaaacgat cgcctctggt ccccgatctg ccgacgctcg ccgaagccgg
     3121 attgcccggc ttcgatatct atacctggtg gggcttcatg gcgccggcgg gcacaccgaa
     3181 ggaaatcatc gccaagtgga acgccgaggt gacgcgcatc ctgaacacgc cggaaatgca
     3241 ggcgttcttc gctcagcagg gcgccgagcc gactccgacg acgcccgagc agttcatggc
     3301 gctgatccag ggcgagattc cgaagtacgc aaagatcatc aaggcgtcgg gcgcgaaggt
     3361 cgattgattc ggacacgggg acatagaacc attctgggcg gaggaaacat gatgcgaata
     3421 ctgcgacgag cagcgctggg cgcgctcgcc ttgctgctcg cgggcggcgc ttgggcgcaa
     3481 ccctatccga ccaagtccat ccggctggtc gtccccttcc ccgcggccgg aaccaccgac
     3541 atcctagcgc gcgaagtcgc gcagcgcctc tccgtgtcgt tcggccaatc ggtcgtcgtc
     3601 gataatcgcc caggggcagc cggcaatatc gggtcggata tggtggccaa gagcgcaccg
     3661 gacggctaca ccctgctgat ggggacggtc gggacgcacg cgatcaatcc cagcctgtac
     3721 gccaagatgc cctataacca cgtgaaggat ttcgtcccga tcgttctggt cgccggcgtg
     3781 cccaacgtcc tcgaggtcac gccgagcctg ccggtcaatt cggtagccga cctgatcaag
     3841 ctcgccaagg aaaaacccgg tcagctcaac ttcgcttcgt cgggaagcgg cacatcgatc
     3901 catctttcgg gcgagctctt caagacgatg accggcgtcg acatgatgca cgtgccgtac
     3961 aagggcagcg ctcccgccct caccgacttg atgggcggac aggtgcagct gatgttcgac
     4021 aacctcccgt cgtcactggc gcagatcaag gccggcaaac tgcgcgcgat cgcggtcacc
     4081 tcggcgcagc gctcgccggc gctgccgaac attccgacga tcgccgagag cggattgccg
     4141 gggttcgagg ccagctcgtg gttcggcctg ctggcgccgg cagggacgcc gccggcgatt
     4201 gtcgcgcggg tcaatgctga cgttaacgag tggttgcaga ccgccgaggc gaaggagaag
     4261 ctgcttgccc aaggcgctat cccggcgggc ggcacgcccg agcagttcgc cgcgcacatc
     4321 cgcgtcgaaa ccgaaaaatg ggccaaggta gtgacggttt cgggcgcgaa ggtcgattga
     4381 tggctcaacg cagccatgca acccaggcga cgaccagcca cgcgacgatc gagaggacgc
     4441 cgcccagcgg cgtgatcgcg ccgagccagc gcg
//
LOCUS       DATANS010000563         2085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_27890, whole genome shotgun sequence.
ACCESSION   DATANS010000563 DATANS010000000
VERSION     DATANS010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27890"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VHT22_08275"
     CDS             <1..832
                     /locus_tag="VHT22_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3137591.1"
                     /translation="GQTPFDSISPAVRAALLAACAVVAATILYFYALLLYASLVQALG
                     ANHALTLRHYRVIFTEGLRVIRDTLIIAGLAMPLGGLYGILVGYLVARKPFVGRRTME
                     LVSMINYALPGTIVGIAYLIAFNDPPIELTGTALIIIACYVFRYGATGIRATVAMLQQ
                     IDKSLEEASQGLGAGSGTTFRRVTLPLILPAFFTGLGVVFIRSMTAISATIFLVSISW
                     TLITVRILENITELSLGPAAAYSVLVVGIVFVVIALIGRGLRWFRAPGAIPVTSMFGG
                     "
     gene            837..1934
                     /locus_tag="VHT22_08280"
     CDS             837..1934
                     /locus_tag="VHT22_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3137592.1"
                     /translation="MAIETIGVQVDGVSKRFSHRVKGEIYAARDVHLDVAPGEFLTLL
                     GPSGCGKTTTLRMIAGFEHPDAGSIRLGGQDVTGLPANQRNMGFVFQNYALFPHLSVF
                     DNVAYGLQVRGQAAAVIAKSVGDVLALVGLSGYEHQFSGQLSGGEQQRVALARAIVIR
                     PRVLLFDEPLSNLDAKLRVQMRTEIRDLQRSLAITTIYVTHDQEEAMAVSDRIAVMSQ
                     GSVVQEGTAEDLYYRPASEFVATFVGRVNLVPGTLIERSGDTVTVAALGTTIRAGTAH
                     AAVTGGAAVKLVMRPEVLELVEVGAGDFRADVISCTFLGEKTEYLLRCGDVELQCVRH
                     NAGAVGAIREGSAVGVRVAASAVTVLPEAAR"
BASE COUNT          323 a          674 c          694 g          394 t
ORIGIN      
        1 cgggcagaca ccgttcgaca gcatctcgcc ggccgttcgt gctgcgctgc tcgccgcctg
       61 cgcggtggtc gctgcaacga tcctctactt ctacgccttg ctgctgtacg cgtcgctggt
      121 gcaggcgctg ggcgccaacc acgcgctgac gctgcggcac tatcgcgtga tattcaccga
      181 gggactgcgg gtgattcgcg acacgctgat catcgccggc ctggcgatgc ccctcggcgg
      241 gctctatggc atcctcgtcg gctacctcgt tgccagaaag ccgttcgtcg gacggcgcac
      301 gatggagctc gtgtcgatga tcaactacgc gcttccgggc acgatcgtcg gcatcgctta
      361 cctgatcgcg ttcaacgatc cgccgatcga gctcaccggc acggcgttga tcatcatcgc
      421 gtgctatgtc tttcgctacg gcgccaccgg tatccgcgcc accgtcgcga tgctgcagca
      481 gatcgacaag agtctcgaag aggcttcgca gggcctgggc gctggcagcg gcacgacgtt
      541 tcgccgcgta accttgccgc tgatcctgcc ggcattcttc accggcctcg gcgtggtctt
      601 catccggtcg atgaccgcga tcagcgcaac gatttttctg gtgtcaatca gctggacgct
      661 gatcaccgtc agaatcctcg agaacatcac cgaattgtcg ctagggccgg cggcggcgta
      721 ctcggttctg gtcgtcggca tcgttttcgt cgtaatcgcc ctgatcggcc gcgggctgcg
      781 ctggttccgc gcacccggcg cgataccggt gaccagcatg ttcggaggtt gacgcaatgg
      841 cgatcgaaac catcggcgtc caggtcgacg gcgtatcgaa acgcttttcg caccgcgtga
      901 agggtgaaat ctacgcggcc cgggacgtgc acctggacgt ggcgccgggc gagttcctga
      961 ccttgctggg cccgtccggc tgcggcaaga ccacgacctt gcggatgatc gcgggattcg
     1021 agcatcccga cgccggcagc atccgtctgg gcggccagga cgtcaccggc ctgccggcga
     1081 accagcgcaa tatgggtttc gtcttccaga actatgcgct gttcccgcat ctgtcggtgt
     1141 tcgacaatgt cgcctacggt ttgcaggtcc gcgggcaggc cgctgcggtg attgcgaaga
     1201 gcgtcggcga cgtgctggcg ctggtaggct taagcggcta tgagcatcag ttctccggac
     1261 agttgtcggg cggcgaacag cagcgggtcg ctctcgcgcg cgcgatcgtg atccgaccgc
     1321 gcgtgctgct gttcgacgag ccgctgtcca acctcgacgc caaattgcgc gtgcagatgc
     1381 gcacggaaat tcgcgatctg caacgcagcc tggcgattac gacgatctat gtcacccacg
     1441 atcaggagga agcgatggcg gtctctgacc ggatcgcggt gatgagccag ggcagcgtcg
     1501 ttcaggaagg gaccgcggag gatctctatt accgtccagc ttcggaattc gtcgcgacct
     1561 tcgtcggtcg cgtcaatctc gtgcccggaa ccctcatcga gcgcagcggc gacaccgtga
     1621 ccgtggccgc gctggggacg acgatccgag ccggaaccgc gcacgccgcc gtgaccggcg
     1681 gagccgcggt caagctggtc atgcgacccg aggttctcga actggtcgag gtcggcgccg
     1741 gagacttccg cgccgatgtg atctcgtgca ccttcctggg agagaagacc gaataccttc
     1801 tgcgctgcgg ggacgtcgag ctgcagtgcg tccgccacaa cgccggcgcc gtcggcgcaa
     1861 ttcgggaggg ctcggccgtc ggcgtgcgcg tcgccgcgag cgccgtgacg gttctgccgg
     1921 aagctgcccg atgaaggcct gttggctgct cgcagcgctt tgctgttgtg cgggcgcggc
     1981 tttcgcgcag gcgccgcgcg aatcgcacgg cagccacgat gcctacgccg cgccgggggt
     2041 cgcgctggcc tggggggtgc tgcgcggatc agacgaagct tcgac
//
LOCUS       DATANS010000564         6548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_69909, whole genome shotgun sequence.
ACCESSION   DATANS010000564 DATANS010000000
VERSION     DATANS010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6548
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69909"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            409..927
                     /locus_tag="VHT22_08285"
     CDS             409..927
                     /locus_tag="VHT22_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX3137593.1"
                     /translation="MTTRVAPITPGTRPELAAVEAAILAERGEILLLYRVLLNSAPLA
                     AGWEQMLTAVRNRSSLPADMRELVILRVAVLNRAPFEFDAHLPLARKAGIGEAKIAAL
                     QAEEIGDGFTALERAVLTLTDTMTRSVQVPDAQFEPLRTHFDPRGLVELVATVAAYNM
                     VSRFLEALNIGH"
     gene            complement(977..2239)
                     /locus_tag="VHT22_08290"
     CDS             complement(977..2239)
                     /locus_tag="VHT22_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137594.1"
                     /translation="MLLSMLLHATIVILFGTSHGGGAGRGEAMLDVLDVTFRREPSPP
                     GAGLLAGPGAETATGRALLQRPEPQPAPIGPGRGEAMPAIEPAKTSEPTLVEPTMPAS
                     GPTQPEAALPPAAEPLPRIDLRAPQEVDKPLIAPVAPPTIERLAPPPNPPALAAPLDI
                     APPAIPLAPAAPIERLSAPTIQHELAPPIELPPRFVPAPPPSVPAPIERAAVPAPPKA
                     VAPPVEVPPSEAPIVPPPIDRLTAPRIDHELAPSPNLVAPTPTPSVAPLAAPAGTPSP
                     AERAAPSAGAKPAPASEAAAPAAVPGARAAPTAAEPPRLRLGAPGPDEDMFKPRRDVA
                     APPGESGAPRIDMETARQRAREIASEATSTRGILPALPPPPERLSKESHALEKAVKPD
                     CRTAYAGMGLLAVPVLVASTIGDGSCRW"
     gene            complement(2941..3654)
                     /locus_tag="VHT22_08295"
     CDS             complement(2941..3654)
                     /locus_tag="VHT22_08295"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEX3137595.1"
                     /translation="MSTTPAVHAARRWIGLVALASLAATAGSAFAQSEQDQLVRDAQT
                     TFSNFVRDPDMTWFQRHLGEAKAVLIAPKIVRAGWIFGGSGGRAVLFSRNAQSGRWSG
                     PAFYNIATASVGFQAGIDVSETVTLVMTDKGLNSLMASSVKLGGDASVAAGPIGAGAK
                     SDVTADLIAFSRAKGVYGGLNLDGTVVHVADNWNQAYYGRSVQPPDILMTARAHNPNA
                     DPLADTISRAAGGAKTSLR"
     gene            complement(3899..4603)
                     /locus_tag="VHT22_08300"
     CDS             complement(3899..4603)
                     /locus_tag="VHT22_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX3137596.1"
                     /translation="MASSTITVATYNIHDAVGGDGKFSPERIVAVLAEIDADIIALQE
                     VGSDHDGVDTLERLRHATGLKAVSGPARWRAAGAHGNCLLSRHPIVECTHLDLTYQRR
                     EARSAIDAVIDCEGTLLRVLATHLGLRPAERRAQVRSLLKALEAETPHPTLLMGDLNE
                     WFLWGRPLRWLHRHFRKTPSPRSFPARAPLLALDRIWVEPRVLLQRLWVHASPAARAA
                     SDHVPVVADISLPDAL"
     gene            complement(4611..>6548)
                     /locus_tag="VHT22_08305"
     CDS             complement(4611..>6548)
                     /locus_tag="VHT22_08305"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX3137597.1"
                     /translation="LNALLNEHPQLQIYVLSWDFALLFALEREWLGRLKFGWPKRHRL
                     HFELDDHHPTAASHHQKLVVVDDALAFVSGFDLTRNRWDTPAHLPADPARRNADGSSY
                     GPFHDVGALVDDEAARALGELARERWYRATGAHAREREGTPGAAAWPAQAEIALGDVD
                     VGIVRTEPAFDGAPAVAEVRQLHLDAIAAAKTSIFAENQYFTSPLIADAFESRLVEPA
                     GPEIVVVSPMTQSGWLEVSTMGVLRARVHRRLVQADARGRYRLYCPWLGDDAPPQARC
                     LNVHSKVLVIDDEIVSVGSANLSTRSMSLDTECNLVIAANGEPRVRAAIAQLKSRLLG
                     EHLHREPSMVAAAIEKHGSLIDAIEALRDKGRSLRPLGVTDEDEWSTVLADQPMIDPA
                     EPVDVDSLLSDVVARHERKPASGRAVAIALIVFALAALALAWRYTPLREWINVESLVH
                     LSDRFEASPFAPLIVIGGFVLGGLLVIPLTMLIAATGIVFGPWLGMLYSLLGATLSAV
                     FVYGIGREVGRDAVRRVAGRRINDLSRRIAKRGLLAMLFVRIVPIAPFSIINLVAGAS
                     HLGFRDFLLGTILGLLPGTILISFFVDRILAAVRHPGPVTFALLALVAGVAIAGTLAV
                     RARLKRGEAADASDQRAPGAN"
BASE COUNT         1086 a         2143 c         2268 g         1051 t
ORIGIN      
        1 cgacgattca ccgcgacatc atgttgaact ctccgttatc gcgaagagat tcggcagcag
       61 gtcgtagcgc attcctcctc ccatcgaagc gcaactcggc agcatcccta cggcatagac
      121 cattgctacc gcgataaccc atctcgacat gcggcggatc ggttccatga gagttctccc
      181 gcaagctggt gagggcgccg tcgatcgctc gcgatagcgc atcgtagttg ccaacagcgg
      241 ccctgcagcc gctattccag ccgtaagaac gtggtggcgt ggttcgtgtc tttgggcgaa
      301 ctgctagcat cggccttgca gcagcggtcg gagcggacaa tcttccgcgc aatcgaccgg
      361 ttgcgatttc gcctatggcg gcattaacaa cgttggaatc aaacgaaaat gacgacacgc
      421 gttgcgccga tcacccccgg gacccgcccc gagcttgcgg cggtagaggc cgctatcctc
      481 gccgagcgcg gcgagattct tctgctctac cgggtcctgc tgaacagcgc gccactcgcc
      541 gcgggctggg agcagatgct gacggcggtg cgcaaccgca gctcacttcc cgccgatatg
      601 cgcgagttgg tgattcttcg ggtggccgtg ctcaatcgtg cgccgttcga attcgacgcg
      661 catttgccgc tggcgcgcaa ggccgggatc ggcgaagcga aaatcgccgc gttgcaggcg
      721 gaggaaatcg gcgacggatt cacggctctc gaacgcgcgg tgttgacgct gaccgacacg
      781 atgacccgta gcgtgcaggt tcccgatgcg caattcgagc cgttgcgcac tcacttcgat
      841 ccgcgtggat tggtcgagtt ggtggcgacc gtggccgcat acaacatggt ctcgcgcttc
      901 ctggaagcgc tcaatatcgg gcactgactc ttagccgcgc gcgtgagcgc ggggcgctgg
      961 ccggtcagcg ccgaggctac cagcgacagc tgccgtcgcc gatggtgctc gccaccagca
     1021 ccgggaccgc gagcaggccc atgccggcat aagcggtgcg gcagtcgggc ttgaccgcct
     1081 tttccagcgc gtggctttcc ttagacagcc tttccggcgg cggcggcagc gcaggcagga
     1141 tgccgcgcgt gctcgtcgct tcgctcgcta tttcgcgcgc gcgttggcgc gcagtctcca
     1201 tgtcgatgcg aggggcgccc gactcgcccg gcggcgccgc gacatcgcgc ctcggtttga
     1261 acatatcttc gtcggggccg ggcgcaccca gccgcagtcg aggcggctcc gcggccgtag
     1321 gcgcggcgcg cgcgccgggg accgccgccg gcgcggcggc ctccgaggcg ggcgccggct
     1381 tggcccccgc ggatggcgct gcccgctcgg ccggcgaagg cgtcccggca ggcgccgcca
     1441 gcggtgcgac ggatggagtt ggagttggtg cgaccaggtt cggcgatggc gccagctcat
     1501 ggtcgatccg cggggcggtg agacgatcga tgggcggcgg cacgatcggc gcctcactgg
     1561 gtggaacctc gacgggcggc gctacggcct tcggtggtgc cggcaccgcc gcgcgctcga
     1621 tcggcgcagg gacagaaggc ggaggagccg gcacgaaccg aggcgggagc tcgatcggcg
     1681 gggcgagttc gtgctgaatc gtgggcgccg acagtcgttc gatcggcgcc gcgggggcca
     1741 gcggaattgc gggcggcgcg atgtcgagcg gcgcggcgag cgcaggcgga ttcggcggcg
     1801 gggccaggcg ctcgattgtc ggcggcgcaa ccggcgcgat cagcggctta tccacctcct
     1861 gcggcgcgcg caagtctatg cgcggcaagg gctcggccgc tggcggcaga gcggcttcgg
     1921 gctgcgtcgg ccccgatgcg ggcatagtgg gctcgaccag ggtcggctcg ctggtcttcg
     1981 ccggttcgat ggcgggcatc gcctcgccgc ggccgggccc gattggcgcc ggctggggtt
     2041 cggggcgctg cagcaaagcc cgtccggtcg ccgtctctgc gcccggtccc gccagcaggc
     2101 cggcaccggg cggactcggc tcgcgccgga aggtcacgtc aagcacatcg agcattgctt
     2161 cgccgcggcc ggcgccgccg ccatgcgagg tgccgaaaag aatgacgatg gtggcatgca
     2221 gcagcatcga gagcagcacg aaccgcacga gggccggttg gggcgcgtcg gctcgccatt
     2281 gggcccgctt tgccgaactt cgtgacgtga tcactcgttt gtttcgcttg cgatgccgga
     2341 cagaacggct gttagcttac ctcgttcgcc gaccgggcga ccgcggtccc gccagcaccc
     2401 cgggcaaagc ccgcgtatgt cgggcacggg atttgcgggg taaccggtgt agacacgccc
     2461 gggcgagtgg cagcgagcac ccgatgccgg aaggcgagtc gaaacgattg ccaggcgctt
     2521 tggtcagcaa tatcaagggc gaatcgaaac aggggcgccg ttgaaagtcc ggacgaggcg
     2581 aggctgggcc agtgacgcaa tgcgtcgcaa tgcagcgtat ttggtgcagt gcgggccggg
     2641 tcctggcgac gagatacggc tagaacgcgg tgggttggat caggagatgc tcgatgcgga
     2701 attcgctcta tagacggcac gcgcagtcgc tcgaccgctg gggaggcggc ggcaacggca
     2761 ggactgcgat ggcgcgcgtc cttggcacga tgggctcgct caagtcggcg cgcgaccgcg
     2821 cgctgccggc gcggccgcgc gagctcaccg gcgccaccgt ccaaacaaag cggctcgaag
     2881 ctgaagctcc gagccgcctt cgttacttgc gccgcggcga tcgccggcgc atgacttgcg
     2941 tcaacgcaga cttgtcttgg cgccgccggc cgcgcggctg attgtatcgg ccagcgggtc
     3001 cgcgttcgga ttgtgcgccc tcgcggtcat cagaatgtca ggcggctgaa cgctgcgtcc
     3061 gtaataagcc tggttccaat tgtcggcgac gtggacgacg gtaccatcca ggttcaagcc
     3121 tccgtagaca cccttggcgc ggctgaacgc gatcaagtcg gcggtcacat cgctcttcgc
     3181 accggctccg atcggacccg cggcaacgct cgcatcaccg cccagcttca ccgaacttgc
     3241 catcagcgaa ttcaggccct tgtcggtcat gaccagagtg acggtttccg aaacgtcgat
     3301 accagcctgg aaacctacgc tcgcggtagc gatgttatag aacgccggac ctgaccagcg
     3361 gccgctttgc gcattgcgcg aaaacagcac cgcgcgtccg cccgaaccgc cgaatatcca
     3421 gcctgcgcgc acgatcttcg gcgcgatcag caccgccttc gcttctccga ggtgccgctg
     3481 gaaccacgtc atgtccggat cgcggacgaa attcgagaat gtcgtctgtg cgtcgcggac
     3541 caattgatcc tgctccgatt gcgcgaacgc cgatccggcc gtcgccgcca gcgatgcgag
     3601 agcgaccagc cctatccaac gtcgtgcagc gtggactgct ggagttgtac tcatatgcct
     3661 tcctttcgag attgattgcc cgcgcgcgca ccgtgatccg cgcggcgcgg ctgctgcatc
     3721 gcgctgccga gacggccacg cgatgaccgg cgcttgcact atcgcgatgc agaagcagca
     3781 cgcgaaagtg ttggccccgg tattcaagca tgagcgaatg agccccgcga cggtatcggc
     3841 ggggcgctga tttggctgta ggaccggcgg cgacagcgcg cggctatgca aacgccgatc
     3901 aaagcgcgtc gggcagactg atgtcggcga cgaccggaac gtggtcggat gccgcacgcg
     3961 cagcgggact ggcgtgcacc cacaatcgct gcaacagcac gcggggctcg acccagatgc
     4021 gatccagcgc cagcagcggt gcgcgcgcgg gaaagctgcg cggggatggc gtctttcgga
     4081 aatggcggtg cagccagcgc agcggacggc cccacagaaa ccactcgttc aaatcgccca
     4141 tcagcaaggt gggatgcggc gtttccgctt ccaacgcctt gaggaggctt cgcacctgcg
     4201 cgcgccgctc ggcgggacgc aagccgagat gcgtcgccag tacacgcagc agcgtacctt
     4261 cgcaatcgat caccgcatcg atcgcgctgc gcgcctcgcg gcgctgatag gtcagatcga
     4321 ggtgcgtgca ttcgacgatc gggtgccgcg acagcaggca attgccgtgt gcgcctgcgg
     4381 cgcgccagcg cgcggggcct gagacggctt tcagcccggt ggcgtggcgc agtcgctcca
     4441 gcgtgtcgac gccgtcgtgg tccgagccga cttcctgcag cgcgatgatg tcggcgtcga
     4501 tctcggcgag cacggcgacg atgcgctcgg gtgaaaattt tccgtcgccg ccgaccgcgt
     4561 catggatgtt gtaggtcgcg acggtgatgg tcgaggacgc catcgcgcgg tcagttggcg
     4621 ccgggtgcgc gttgatcgct cgcgtcggca gcttcgcccc gcttgaggcg cgcgcgaacc
     4681 gccagcgtgc cggcgatcgc gacgccagcg acaagcgcca gcagcgcgaa tgtgaccggt
     4741 cccgggtgcc gcactgccgc gaggatgcgg tcgacaaaaa agctgatcag aatcgtgccg
     4801 ggcaaaaggc ccaggatcgt gccgagcaga aagtcgcgaa agcccaggtg cgatgcgccg
     4861 gccaccaggt tgatgatcga aaaaggcgcg atgggcacga tgcgcacgaa cagcatcgcg
     4921 agcagcccgc gcttggcgat gcggcgggag agatcgttga tccgccgccc ggcgacccgc
     4981 cgcaccgcgt cgcgcccgac ctcgcgcccg atgccgtaga cgaataccgc actgagcgtc
     5041 gcgcccagca gcgaatagag catgcccagc catgggccga acacgatgcc ggtcgcggct
     5101 atcagcatcg tcaacggaat caccagaagt ccgccgagga cgaagccgcc gatgacgatc
     5161 agcggtgcga acgggcttgc ttcgaaccgg tcgctcaagt gcaccagtga ctccacgttg
     5221 atccattcgc gcagcggcgt ataccgccac gccagcgcca gcgcggcgag cgcgaacacg
     5281 atcagcgcga tcgccacggc gcgccccgaa gcgggcttgc gttcatggcg ggcgacgaca
     5341 tcggacaaca ggctatcgac gtcgaccggc tccgcgggat cgatcatcgg ctgatcggcc
     5401 agcacggtgc tccactcgtc ctcgtctgtg acaccgagcg gacgcagcga gcggcctttg
     5461 tcgcgcagcg cttcgatcgc gtcgatcagc gatccgtgct tttcgatcgc cgccgcgacc
     5521 atcgatggtt cgcgatggag atgctcaccg agcaggcggg atttgagctg cgcaatcgcc
     5581 gcgcgcactc gcggctcgcc gtttgccgcg atcaccagat tgcattcggt gtcgaggctc
     5641 atcgagcggg tactgaggtt ggccgagccg accgagacga tttcgtcgtc gatgaccagc
     5701 accttgctgt gtacgttgag gcagcgtgcc tgcgggggcg catcgtcgcc cagccatgga
     5761 caataaagcc ggtagcgccc gcgggcgtcg gcttgcacca ggcgccggtg cacgcgcgcc
     5821 cgcagtacgc ccatcgtcga gacctcgagc cagccgcttt gcgtcatcgg cgaaacgacg
     5881 acgatttccg gacccgccgg ctcgaccagc cgcgactcga atgcgtcggc gatcagcggc
     5941 gaggtgaaat actggttttc cgcaaagatc gacgtcttcg cggcggcgat cgcatccaga
     6001 tgcagctgtc gcacctctgc gacggcaggc gcgccatcga acgctggctc ggtgcgcacg
     6061 atcccgacgt cgacgtcgcc gagcgcgatc tcggcttgcg ccggccatgc ggcggccccg
     6121 ggggtgcctt cgcgctcgcg cgcgtgtgct ccggtggcgc ggtaccagcg ctcgcgcgcc
     6181 agctctccca gcgcacgcgc ggcctcgtcg tcgaccagcg cgccgacgtc gtggaatgga
     6241 ccgtagctgc tgccgtcggc gttgcgccgt gccggatcgg ctggcaggtg ggccggcgtg
     6301 tcccagcggt tacgcgtgag gtcgaagccg ctgacgaacg ccagcgcatc gtcgacgacg
     6361 accagcttct gatggtgcga agcggcggtc ggatgatgat catctagctc gaaatgcagg
     6421 cggtggcgct tgggccaacc gaacttgagg cgacccagcc attcgcgctc gagcgcgaac
     6481 agcagcgcga agtcccagct cagcacgtag atctgaagct gcggatgctc gttgagcagc
     6541 gcgttgag
//
LOCUS       DATANS010000565         2917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_69915, whole genome shotgun sequence.
ACCESSION   DATANS010000565 DATANS010000000
VERSION     DATANS010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69915"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1175)
                     /locus_tag="VHT22_08310"
     CDS             complement(30..1175)
                     /locus_tag="VHT22_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3137598.1"
                     /translation="MQLSDSQQMVRETMRDFAQQRLAPFAAAWDREHAFPRDALRELA
                     ALGACGIVVPESYGGAGLDYVSLALALEEIAAGDGATSTVISVQNSVVCGPIFAFGTA
                     AQKDRYLKPLASGAKIGCFCLTEPHTGSDAAAIATRAEFIRGDDGGHYVLNGTKQFIT
                     SGKNADVAIVFALTDKAAGKKGMSAFIVDTALPGYDAARIEDKLGQRASDTAQIVLSD
                     CRVPAANLLGREGEGYRIALANLEAGRIGIAAQAVGMARAAFEAALSYARERESFGKP
                     IIEHQAISFRLADMATQIEAARQLTWHAASLRDAGLPCLKEASMAKLFASEMAERVCS
                     DAIQIHGGYGYVTDFPVERIYRDVRVCQIYEGTSDIQRLVIGRALAG"
     gene            complement(1188..1820)
                     /locus_tag="VHT22_08315"
     CDS             complement(1188..1820)
                     /locus_tag="VHT22_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX3137599.1"
                     /translation="MPTAQNLALFALASMLLALTPGPNLLYLVSRTLCQGRRAGIISL
                     AGTSLGFVFHVLAAALGLSAIFLTVPVLYDAVRYAGAGYLLWLAWDALRPGGRDVFAP
                     RALPQDSPWRLFRMGLVTSLLNPKVAMFYLALFPQFIDAARGSVLAQGLVLGLLQIVI
                     SSASDLVFVLAAGAITGWLARRPLWSAVQRWVLGVVLAGIAVRLAFDSRR"
     gene            complement(1894..2856)
                     /locus_tag="VHT22_08320"
     CDS             complement(1894..2856)
                     /locus_tag="VHT22_08320"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX3137600.1"
                     /translation="MADKLVFESPRVTLRIGGKPAPSAAPRGARDPFPVIVGCPRSGT
                     SLLAVMLDAHSQLAIPPETAFLPNVMGLVGDSASLRRRFFDIVTADRMTISNWSDFGL
                     DKDLFWRRLQAISAFTVAAGTRAFYALYGETQRKPRSGDKTPGYVFTMQHIQALLPEA
                     RFIHVIRDPRDTAMSWRKTWFAPSQDYATLGSFWRQNVEVGRHAASSLHHYQEIRFED
                     LVLHPEAELRRLCSYLSLAYEPAMLDSGARGQERLARLQGRAHVDGRMIARDERTRIH
                     VNLARPPMAERVEVWRREMSDAERRQVESGAGPLMRELGYEAGA"
BASE COUNT          521 a          989 c          970 g          437 t
ORIGIN      
        1 cgatggcggc agtcatgcga tcctttcggt tagccggcga gagcgcgccc gatcaccaga
       61 cgctggatgt cgctggtgcc ctcgtagatc tggcagacgc gcacgtcgcg atagatgcgt
      121 tcgaccggga agtcagtcac atagccatag ccgccatgga tctggatcgc gtccgagcac
      181 acacgctcgg ccatttccga tgcaaaaagc ttcgccatcg aggcttcctt caggcacggc
      241 aggcccgcgt ctcgcaagct cgccgcatgc caggtgagtt gccgggcggc ttcgatctgc
      301 gtagccatgt cggcgagccg aaaactgatc gcctgatgct cgatgatcgg cttgccgaag
      361 ctttcccgct ctcgcgcata ggacagcgcc gcctcgaaag cggcgcgtgc catgccgacg
      421 gcctgcgcgg cgatgccgat gcggccggcc tcgagattgg ccagcgcgat cctgtacccc
      481 tcgccttcgc ggcccagcag gttggcggcc ggaacccggc agtcggagag cacgatttgt
      541 gcggtatccg aggcgcgctg tccgagcttg tcctcgatgc gcgccgcgtc gtatcccggc
      601 aacgcggtat cgacgatgaa cgccgacatg ccttttttgc cggcggcctt gtcggtcagc
      661 gcaaacacga tcgcgacgtc ggcattcttg cccgaggtga tgaactgttt ggtgccgttc
      721 agcacatagt gcccgccgtc gtcgcctcga atgaactccg ctcgcgtggc gatggccgcc
      781 gcgtccgagc cggtatgcgg ctcggtcagg cagaagcagc cgatcttggc gccgctcgcc
      841 agcggcttca gataacggtc tttctgcgcc gcggtgccga acgcgaatat cgggccgcag
      901 acgacggagt tctgcacgct gatgacggtc gaggtcgcgc cgtcgccggc ggcgatttcc
      961 tccagcgcca aggcaagcga tacgtagtcc aggccggcgc caccgtacga ctcgggcacg
     1021 acgatgccac aggcgccgag cgccgcgagc tcacgcaacg cgtcgcgcgg aaacgcgtgc
     1081 tcgcgatccc atgccgcggc gaacggcgcc agccgctgtt gcgcaaagtc gcgcatcgtc
     1141 tcgcgcacca tctgctggga atcgctgagc tgcatcgaaa aggccgccta ccgtcgcgag
     1201 tcgaaggcga gccgtaccgc gatgccggcc agcacaacgc cgagcaccca gcgctgcacc
     1261 gccgaccaca gcgggcgtct ggcaagccag ccggtaatcg cgccggcggc gaggacgaag
     1321 acgaggtcgc tcgcggagct gatcactatt tgcagcagcc cgagcaccaa gccctgggcc
     1381 agcacgctgc cgcgagcggc atcgatgaac tgcggaaaca gcgccagata gaacatcgcc
     1441 actttcggat tcaacagact ggtcacgagg cccatgcgga agagccgcca cggcgagtcc
     1501 tgcggcagcg cgcgcggcgc gaacacgtcc cgccctccgg gtcgcaacgc gtcccaggcg
     1561 agccacagca ggtagccggc gccggcatag cgaacggcat cgtagaggac cggcacggtc
     1621 aggaaaatcg ccgacaggcc gagcgcggcg gccaagacgt ggaacacgaa gccgagcgag
     1681 gtgccggcga gcgagatgat gccggcgcgc cgtccctggc agagggtccg cgacaccagg
     1741 taaagcaggt tcgggcccgg cgtcagcgcc agcagcatgc tcgccagcgc gaacagcgcg
     1801 aggttctgcg ccgtcggcat cagccgcttt ccgcttcgcc cggacgagcg agaccgacgg
     1861 cagcgccggc aacctggccc cgcgcgagcg gcctcaagcg ccggcctcgt agcccaattc
     1921 gcgcatcagc ggtccggcgc cgctttccac ctgacggcgt tcggcatcgc tcatttcgcg
     1981 ccgccacacc tcgacgcgct cggccatcgg cggccgcgcg agattgacat gaatgcgcgt
     2041 gcgctcgtcg cgcgcgatca tccggccgtc gacgtgcgca cgaccttgca gtctggccag
     2101 ccgttcctgc ccgcgcgcgc cggaatcgag catcgccggc tcataggcca gcgacaggta
     2161 cgaacagaga cgcctgagct cagcttccgg gtggagcacc aggtcctcga agcggatctc
     2221 ctggtagtgg tgcagcgagg acgccgcgtg ccggccgact tcgacgttct gccgccagaa
     2281 cgatccgagc gtcgcgtaat cctggctggg cgcgaaccag gtcttgcgcc atgacatcgc
     2341 ggtatcgcgc ggatcgcgta tcacgtgaat gaaacgcgct tcgggaagca gtgcctggat
     2401 gtgctgcatc gtgaagacgt aaccgggggt cttgtcgccg ctgcgtggtt tgcgctgggt
     2461 ttcgccgtac agcgcgtaga acgcccgggt gcccgcggca acggtgaaag cggagatggc
     2521 ctgcagcctg cgccagaaca ggtccttgtc gagaccgaag tcagaccagt tcgaaatagt
     2581 catccggtcg gcggtgacga tgtcgaaaaa gcgccgtcgc agcgacgccg aatcgccgac
     2641 cagccccatc acgttgggga ggaacgcagt ctccggcgga atcgccagct gcgaatgcgc
     2701 gtcgagcatc actgccagca acgaggtgcc cgagcgcgga catccgacga tcaccggaaa
     2761 aggatcgcgc gcgccgcgcg gcgctgccga cggcgccggc ttgcccccga tgcgcaaggt
     2821 gacgcgaggc gactcgaaga cgagcttgtc ggccatcgcc ttgtcgtcag gcgagctcta
     2881 tcgcgagcgc ggtcgcttcg ccgccgccga tacaaag
//
LOCUS       DATANS010000566         1760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_658957, whole genome shotgun sequence.
ACCESSION   DATANS010000566 DATANS010000000
VERSION     DATANS010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658957"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VHT22_08325"
     CDS             <1..485
                     /locus_tag="VHT22_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012252530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3137601.1"
                     /translation="RIYSEVSAGTTVKLYLPRYRGAVEAAAASEVAEGAAKAHDGETV
                     LVIEDEDSVRGLVVEVLHDLGYRALEARDGPSGLKVLRSRERIDLLVSDIGLPGMNGR
                     QVAEAARETRPDLKVLFITGYAENAAMSHGFLEPGMEMVTKPFAVDSLAARIREMIGT
                     "
     gene            593..1366
                     /locus_tag="VHT22_08330"
     CDS             593..1366
                     /locus_tag="VHT22_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX3137602.1"
                     /translation="MDIATAAGLVAGAIVIMTLILMGGDLRMFYDIHAIIIIFGGSFA
                     ATLIRFPLAAMLHGLPLGIKFAFTLRSISTRELVDELASIAEVARKSGPVGLEKITPE
                     DPFLAKGVRFVADGYDADFIRDNLERDRDNFLTHLDEGQKIYRAIGDCAPAFGMIGTL
                     IGMVQMFANMTDPSKLGPFMAVALLATLYGALVANLFCLPIADKLHVKLVEEEVNRTL
                     IIDGILMIRESKSPTLVREMLLAYLPEKHRHDEAELAPA"
     gene            1387..>1760
                     /locus_tag="VHT22_08335"
     CDS             1387..>1760
                     /locus_tag="VHT22_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEX3137603.1"
                     /translation="MAKKRGAAHGGHGWFVTFADLMGLLMSFFVMLVAFSTQDKQKLQ
                     IVAGSMRDAFGVQSEARYAGIVESDGLPTRPRLKNVDHIQPEESSNTPTPDQEDRDKT
                     SGAKIKVDRNFALAAASLRQAL"
BASE COUNT          323 a          565 c          551 g          321 t
ORIGIN      
        1 tccgcatcta ttcggaagtc agcgctggca cgacggtgaa actctatctc ccccgctatc
       61 gcggtgccgt cgaggcagcg gccgcaagcg aagtcgccga gggcgccgca aaagcccacg
      121 acggcgaaac ggtgctggtc atcgaggatg aagattcggt gcgtgggctc gtggtcgaag
      181 ttctgcacga tctcggctac cgcgcgctcg aagcacgcga tggcccttcg ggtctgaaag
      241 tcctgcgctc ccgcgagcgc atcgatctcc tggtcagcga catcggattg ccgggcatga
      301 acggacgcca ggtcgctgag gcggcgcgcg agacgcgccc cgatcttaag gttctgttca
      361 taaccggcta cgccgagaat gccgcgatgt cgcacggctt tcttgagccc ggcatggaaa
      421 tggtcacgaa acctttcgct gtcgattcac tggcggcgcg catccgcgaa atgatcggca
      481 cctgacggga cgtcaggtct caacccttca cgagccctta agacccgccg cgctagggtt
      541 tctcatcttg ccggtggggt gtcgcgggtc gcgttggggt ttggggaagt tcatggatat
      601 cgccaccgcc gcagggctgg tcgcaggcgc gatcgtcatc atgacgctca tcctgatggg
      661 cggcgacctg cggatgttct acgacatcca tgcgatcatc atcatttttg gcggttcgtt
      721 cgccgcgacc ctgatccgct tcccgctcgc cgcgatgctg catgggctgc cgctcggcat
      781 caaattcgcc ttcacgttgc gcagcatcag cacgcgcgag ctggtggacg aacttgcgag
      841 catcgccgaa gtcgcgcgca agtcgggccc tgtggggctc gagaagatca cgcctgaaga
      901 tccgttcctc gccaagggcg ttcgcttcgt ggcggacggc tacgacgccg atttcatccg
      961 cgacaacctg gagcgcgacc gcgacaactt tctcacccat ctcgacgaag gacagaagat
     1021 ctatcgcgcg atcggcgact gcgcgccggc gttcggcatg atcggaacgc tgatcggcat
     1081 ggtgcagatg ttcgccaaca tgaccgaccc gtccaagctc ggcccgttca tggcggtcgc
     1141 gctgctcgcg acgctgtacg gcgcgctggt cgcgaacctg ttctgcctcc cgatcgccga
     1201 caagctgcac gtcaagctgg tcgaagaaga agtgaatcgc acgctgatca tcgacggcat
     1261 cctgatgatc cgcgagtcca agagcccgac gctcgtgcgt gagatgctgc tcgcctatct
     1321 gcctgaaaag catcgccacg acgaagccga acttgctccg gcctgagcag gaacggagcg
     1381 cttgcgatgg cgaaaaaacg cggagcagcg cacggcggtc acggctggtt cgtgactttc
     1441 gcggacctca tgggcctgct gatgagcttc ttcgtgatgc tcgtcgcgtt ctccacgcag
     1501 gacaagcaga agctccagat cgtcgccggc tcgatgcgcg acgccttcgg cgtgcagagc
     1561 gaggcgcgct acgccggtat cgtcgagtcc gacggcctgc cgacccgccc gcggctgaag
     1621 aacgtcgacc acatccagcc tgaggaatcc tccaacacgc cgacgccgga tcaggaggat
     1681 cgcgacaaga cgtccggcgc aaagatcaag gtcgaccgca acttcgcgct cgccgcggcc
     1741 tcgttgcgac aggcgttgca
//
LOCUS       DATANS010000567         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_365196, whole genome shotgun sequence.
ACCESSION   DATANS010000567 DATANS010000000
VERSION     DATANS010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365196"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VHT22_08340"
     CDS             complement(<1..353)
                     /locus_tag="VHT22_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX3137604.1"
                     /translation="MISRPAADPPSDTLAVDVISDVVCPWCYIGKRKLEAALSEIKAR
                     EPALDIAVRWHPFQLNPDMPSGGMPRTSYLAAKFGGTDRAAEVYSRVRAVGAEVGIPF
                     AFERIVMQPNTLEAHR"
     gene            490..>1610
                     /gene="earP"
                     /locus_tag="VHT22_08345"
     CDS             490..>1610
                     /gene="earP"
                     /locus_tag="VHT22_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEX3137605.1"
                     /translation="MTSPQRWDIFCSVVDNYGDAGVAWRLARQLAAEYRCDARLFVDT
                     LPVLARIAPDVDPSRERQKVQGVDILRWNGADRPISPTAPGAAVIEAFGCGLPSSYLD
                     AMIALAPRPVWINLEYLSAESWIEGSHALTSRHPRLPLQRHFYFPGFTTASGGLLREA
                     GLLSRRDAFQADPAARAAFWRSLGVAPPEGALAISLFCYPNRHLPALLDAWADGDLPV
                     WCIVPEGVATGELDRWTGGAVPHAGQVLARGRLSLAGIPFLAQDIYDRLLWACDVNLV
                     RGEDSFVRALWAARPLIWQPYPQPDDAQLAKLDAFLARYGSGMPQPSAASFNAFHRDW
                     NDPVEGNASVDRTWEAFAAELPATGGHARRWTAELATQP"
BASE COUNT          246 a          525 c          551 g          288 t
ORIGIN      
        1 cggtgcgcct cgagcgtgtt cggctgcatg acgattcgct cgaacgcgaa cggtatgccg
       61 acctcggcgc ctaccgcgcg tacccgcgaa tagacttcgg cggcgcgatc ggtgccgccg
      121 aacttggccg caagatagct ggtgcgcggc atgccgcccg aaggcatgtc cggattgagc
      181 tggaatggat gccaccgcac ggcgatatcg agcgcaggct cgcgagcctt gatctcgctg
      241 agtgccgctt cgagcttgcg tttgccgatg tagcaccacg gacaaacgac gtccgaaatg
      301 acgtcgaccg caagcgtgtc cgaaggcggg tcggcggcgg ggcggctgat caaggattcg
      361 aaccgggaaa cgaggagttg ggcgcgacca ttgtagtacc gccgccgcga ttcgtgcgcg
      421 acgcactcga cacgacgacg gcaataggtt caactgtcat cccgagccga tgcgtttttc
      481 ctgccgatcg tgacctcgcc gcagcgctgg gacattttct gctcggtcgt cgacaactac
      541 ggcgacgcgg gcgtcgcgtg gcggctcgcg cggcagctgg ccgcagaata tcgatgcgac
      601 gcgcgcctgt tcgtcgatac tctaccggtg ctggcgcgga tcgcgccgga tgtcgatccc
      661 tcgcgcgaac gacaaaaagt gcaaggagtc gacatcctcc gctggaacgg cgcggatcgt
      721 ccgatctcgc cgacagcgcc cggcgcggcg gtcatcgaag cgttcggctg cggccttccg
      781 tcgtcatatc tcgacgcgat gatcgctctg gcgccgcggc ccgtctggat caacctggag
      841 tacctgtcgg ccgagagctg gatcgaaggc tctcacgccc tgacatcgcg tcatccgcgc
      901 ttgcctttgc aacggcattt ctacttcccg ggattcacga cggccagcgg aggtctgctg
      961 cgcgaggcgg gcctcctttc gcgccgggac gcgttccagg ccgatcccgc cgcgcgcgcc
     1021 gcgttctggc gctcgctggg cgtcgcgccg ccggaaggtg cgctcgcgat ctcgctgttc
     1081 tgctatccga accgacatct gccggcgctg ctcgatgcgt gggccgacgg cgatctgccg
     1141 gtgtggtgca tcgtgcccga aggtgtcgcc acgggcgagc tcgatcgatg gacaggcggt
     1201 gccgttccgc atgcgggaca ggttctcgcg cgcggccgcc tgtcgctggc cggcattcct
     1261 ttcctcgctc aggacatcta tgaccgtttg ttgtgggcct gcgatgtcaa cctggtgcga
     1321 ggcgaggact ccttcgtccg cgcgctatgg gcggcgcgcc cattgatctg gcagccgtac
     1381 ccgcagcccg atgacgcgca gctggcgaag ctcgatgcgt ttctcgcgcg ctacgggtcg
     1441 ggaatgccgc agccaagcgc ggccagcttc aacgcgtttc atcgggactg gaacgatccg
     1501 gtggagggca acgcgtcggt cgatcggacg tgggaggcgt ttgccgccga attgccggcg
     1561 acgggcggcc acgcgcgacg gtggacggcc gagctggcaa cccaaccgga
//
LOCUS       DATANS010000568         1753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_236675, whole genome shotgun sequence.
ACCESSION   DATANS010000568 DATANS010000000
VERSION     DATANS010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236675"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VHT22_08350"
     CDS             complement(<1..1100)
                     /locus_tag="VHT22_08350"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HEX3137606.1"
                     /translation="MKPAGAAHGRRRAVIENISPCVDHGRFAAKRCAGDLVTVEADIF
                     ADGHDRLRALLLVRKSGSAKWTEFDMVALVNDRWCGAFPVPDIGSYEYTIIAWVDRFA
                     SWRHDLARWVAPEDIVLALQAGAALITGLSRRARGADAKALREWAHRIGGDEPVDARR
                     AEALDAALAEIAARHADRSLATTFEPALKLTVDPPRARFSSWYEMFPRSAGDGRTHGT
                     FADCEARLPHIVDMGFDVLYFPPIHPIGLTRRKGKNNALVASLNDCGSPWAIGGSNGG
                     HTAVHRELGTIEDFRQLLAAARTVGIDIALDIALQCSPDHPYISAHPDWFRQRPDGTL
                     QFAENPPKKYEDIYPFDFENEDWRGLWREVRA"
     gene            complement(1097..>1753)
                     /locus_tag="VHT22_08355"
     CDS             complement(1097..>1753)
                     /locus_tag="VHT22_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HEX3137607.1"
                     /translation="FSLWYAFTENFVLPLSHDEVVHGKGSLVNKMPGDEWRQHATLRA
                     VFGYMFAHPGKKLLFMGGEFGQRREWTHDAALEWFVLQHAEHRGIQAWVRDLNRCYRA
                     TPALYQRDFDPAGFAWSDADDAQNSVVGFLRFPAGEGRTLLVACNFTPVPRRNYVLGV
                     PGGGFWREVLNSDATVYGGSGQGNLGGVHAAPLPSHGRPYSLTLSLPPLATVILEQTP
                     "
BASE COUNT          296 a          576 c          600 g          281 t
ORIGIN      
        1 atcgctctga cttcgcgcca caggccgcgc cagtcctcgt tctcgaagtc gaacggatag
       61 atgtcttcgt atttcttcgg cggattctcg gcgaactgca gcgtgccatc cggccgctgc
      121 cgaaaccagt cgggatgagc gctaatgtac ggatggtcgg gcgagcattg cagcgcgatg
      181 tcgagcgcga tgtcgatgcc gacggtgcgt gcggcggcaa gcaactggcg aaagtcctcg
      241 atcgtgccaa gctcgcgatg cacggccgtg tggccgccat tggagccgcc aatcgcccag
      301 gggctgccgc agtcgttgag cgaagcgacc agtgcgttgt tcttgccctt gcgccgcgtc
      361 agtcctatcg gatgaatcgg cggaaagtaa agcacgtcga agcccatgtc gacgatgtgc
      421 gggagccgcg cttcgcaatc ggcgaaggtg ccgtgcgtgc gtccgtcgcc ggccgagcgc
      481 gggaacattt cgtaccagct gctgaagcgg gcgcggggcg ggtcgaccgt cagcttcaac
      541 gccggctcga acgtggtcgc gaggctgcga tcggcgtggc gcgcagcgat ttccgccagc
      601 gccgcatcga gcgcctcggc gcgcctcgca tccacgggct catcgccgcc gatgcgatgc
      661 gcccattcgc gcagcgcctt cgcatcggcg ccacgcgcgc ggcgcgacag tccggtgatc
      721 agtgcggcgc cggcctgcag tgcgaggacg atatcctccg gtgcgaccca gcgggcgaga
      781 tcgtggcgcc atgacgcgaa gcggtcgacc caggcgatga tcgtatattc gtagctgccg
      841 atatccggga ccggaaaggc gccgcaccag cggtcgttga ccagcgcgac catgtcgaac
      901 tcggtccatt tggcggagcc agatttgcgc acgagcagca gggcgcgcaa gcgatcgtgc
      961 ccgtcggcga agatgtcggc ctcgactgtg accaggtcac cggcgcagcg cttggccgcg
     1021 aagcgtccat gatcgacgca gggcgagatg ttttcgatga ccgcgcgccg gcggccgtgg
     1081 gcggcacctg ccggcttcac ggcgtctgct ccagaatgac cgttgccagc ggcggcagcg
     1141 acaaggtcag cgagtacggt cggccgtgcg agggcagcgg cgcggcatgg acaccgccca
     1201 gattgccctg gccgctgccg ccgtagaccg tcgcgtcgct gttcagcact tcgcgccaga
     1261 atccgccgcc cggtacgccg agcacgtagt tgcggcgcgg cactggtgtg aagttgcagg
     1321 cgaccagcaa cgtgcggcct tcgccggcgg gaaagcgcag aaagccgact acgctgttct
     1381 gggcgtcgtc ggcatcgctc caggcaaacc cggcgggatc gaaatcgcgc tgatacagcg
     1441 cgggcgtcgc gcggtagcag cgattgaggt cgcgcaccca ggcctgaatg ccgcggtgct
     1501 cggcgtgctg cagcacgaac cactcgagcg cggcgtcgtg cgtccattcg cggcgctggc
     1561 cgaactcgcc gcccatgaac agcagcttct tgcccggatg cgcgaacata taaccgaaca
     1621 ccgcccgcag cgtggcgtgc tgccgccact cgtcgcccgg catcttgttc acgagcgaac
     1681 ccttgccgtg cacaacctca tcgtgcgaca gcggcaggac gaaattctcc gtgaacgcgt
     1741 accagaggct gaa
//
LOCUS       DATANS010000569         2189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_198472, whole genome shotgun sequence.
ACCESSION   DATANS010000569 DATANS010000000
VERSION     DATANS010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198472"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1602)
                     /locus_tag="VHT22_08360"
     CDS             complement(<1..1602)
                     /locus_tag="VHT22_08360"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX3137608.1"
                     /translation="MHVSSFFLSTLKEAPAEAELASHRLMLRAGMIKRLSAGIYTWMP
                     LGLRVLRKVEAVVREEMNRAGAVELLMPVVQPAELWQESGRWDRYGPELLRLKDRHQR
                     DFILQPTSEEVITDIARKDLKSYRQLPVNLYHIQTKFRDEVRPRFGVMRSREFIMKDA
                     YSFDVDNAGMLKSYRLMYDAYARIFTRLGLEFRAVAADTGQIGGSASDEFQVLADSGE
                     DAIAWCPGSDYAANVELAEAVAPHAHRAAPAEPMQRVPTPGMSTCEDVAALLHLPLGR
                     TVKCIMLHAGGRVHMLLLRGDHTLNEVKVGKLDGLEGFRWASDAEITAATGCAPGYLG
                     PVGVAADMPLIADRTVAVMSDFVCGANETDYHLRGVNFGRDCREPDRVADIRKVVAGD
                     PSPDGNGDLAIARGIEVGHVFALGTVYSVAMGATFLDAAGQPQPMQMGCYGIGITRVV
                     AAAIEQNCDDKGIIWPEPLAPFTIAIAPIGYDRSEAVRAAADRLHDELESAGIEVLLD
                     DRGERPGVMFADLELIGIPHRVTVGD"
     gene            complement(1696..2139)
                     /locus_tag="VHT22_08365"
     CDS             complement(1696..2139)
                     /locus_tag="VHT22_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX3137609.1"
                     /translation="MYKNILVATDGTRLSGKAITHAIELAKALGAGLTAFYASPDYPL
                     PVYAEGAIVEPMTRREYAALCKDEADRILGAVAAKAKSARVAFNAVHTINSTPWRAIL
                     AAARKHRCDVVVMASHGRRGVSAMLLGSETQKVLTHSKIPVIVVR"
BASE COUNT          376 a          713 c          714 g          386 t
ORIGIN      
        1 atcgccgacg gtgacgcggt gcggaatgcc gatcagctcg agatcggcga acatcacgcc
       61 cggccgctcg ccgcgatcgt cgagcagcac ctcgatgccg gccgattcga gctcgtcgtg
      121 caaccgatcg gcggcggcgc gcaccgcctc gctgcggtcg tatcctatag gcgcgatggc
      181 gatagtgaac ggggcgagcg gttcgggcca gatgatgccc ttgtcgtcgc aattctgctc
      241 gatggcggcg gcaacgacgc gagtgatgcc gatgccgtaa cagcccatct gcatcggctg
      301 cggctgaccc gcggcgtcga gaaaggtcgc gcccatcgcc accgaatata cggttcccag
      361 cgcaaagacg tggccgacct cgataccgcg cgcgatcgca agatcgccgt tgccgtcggg
      421 cgacggatca ccggcgacca ctttgcggat atctgcgact ctatccggct cgcggcaatc
      481 gcgtccgaag ttgacgccgc gcaggtgata gtcggtttcg ttggcgccgc agacgaaatc
      541 gctcatcacg gcgacggtcc tgtcggcgat cagcggcatg tcggcagcga ctccgaccgg
      601 cccgagatag ccgggggcgc agcccgtggc ggcggtgatc tccgcgtcgc tggcccagcg
      661 aaatccttcg agcccgtcga gttttccaac cttgacctcg ttgagggtgt ggtccccgcg
      721 cagtaaaagc atatggaccc ggccgccagc gtgcagcatg atgcacttga ctgtgcggcc
      781 cagcggcaga tgcaataacg cggcgacgtc ctcgcaggtg gacatgcccg gtgtcggcac
      841 tctctgcatc ggctcggccg gcgccgcgcg atgcgcgtgc ggcgcgaccg cctcggctag
      901 ctcgacattg gccgcgtaat cggaacccgg gcaccaggcg attgcgtcct cgcccgaatc
      961 ggccagcacc tggaactcgt ccgatgcgct gccgccgatc tggccggtgt cggcggcgac
     1021 cgcgcggaat tcgagcccga gccgagtgaa gatcctggcg tacgcgtcgt acatcagccg
     1081 gtacgacttc agcatgccgg cgttgtcgac atcgaacgag tacgcatcct tcatgatgaa
     1141 ttcgcgcgat ctcatcaccc cgaagcgcgg acgcacctcg tcgcggaact tggtctggat
     1201 gtggtagagg ttcaccggca gctgccggta gctcttcaga tccttgcgcg cgatgtcggt
     1261 gatcacttcc tcggatgtcg gctggagaat gaaatcgcgc tggtgacggt ccttcaaacg
     1321 cagcagctcg ggcccatacc tgtcccagcg tcccgattcc tgccacagct cggccggctg
     1381 gacgaccggc atcaggagct cgacagcgcc ggcccgattc atttcttcgc ggacgacggc
     1441 ttcgaccttg cgcagcacgc gcaatccgag cggcatccag gtgtatatcc cggcggaaag
     1501 ccgcttgatc attccggcgc gcagcatcag gcgatggctg gcgagctcgg cttccgcggg
     1561 agcttccttg agcgtcgaaa gaaaaaaact cgatacgtgc atcggctgcc cggtttttgg
     1621 caaaccatgc attttacgtg aaaggcgcga tcccttcgcg ccggggccgg caatgcagcg
     1681 gcggccctct cggcatcagc gcacgacgat gaccgggatc ttcgagtggg tcagcacctt
     1741 ttgcgtctcg ctgccgagca gcatcgccga gacgccgcgg cgaccgtgcg atgccatcac
     1801 caccacatcg cagcgatgct tgcgggcggc ggcgagaatt gcgcgccacg gcgtcgaatt
     1861 gatcgtatgc acggcattga acgcaacccg cgccgacttc gccttggccg cgaccgcgcc
     1921 gaggatcctg tcggcctcgt ccttgcagag cgccgcgtac tcgcgacgcg tcatcggttc
     1981 gacgatcgca ccctcggcgt acacgggtag ggggtaatcg ggcgaagcat agaaggcggt
     2041 gagcccggcg ccgagcgcct tggccagctc gatggcatgg gtgatggcct tgccggaaag
     2101 ccgcgtgccg tcggtcgcga caagaatgtt cttgtacatt gtggggcctc ctgtcgaaaa
     2161 tgcgccccgc gtgcaggtag gttgcggta
//
LOCUS       DATANS010000570         2552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_112352, whole genome shotgun sequence.
ACCESSION   DATANS010000570 DATANS010000000
VERSION     DATANS010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112352"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VHT22_08370"
     CDS             <1..478
                     /locus_tag="VHT22_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX3137610.1"
                     /translation="GHYVAPNATIIGDVVLESESSIWFNVVIRGDNDTITIGARSNVQ
                     DGSVLHTDAGVPLTLGSSVSVGHLVMLHGCTIGDGSLIGIKAVILNGARIGRDCLIGA
                     NTLIAEGKTIPDRSLVLGSPGRVMRTLDDGEVAGIRAIADHYVDNARRYRADFKPI"
     gene            557..1273
                     /locus_tag="VHT22_08375"
     CDS             557..1273
                     /locus_tag="VHT22_08375"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEX3137611.1"
                     /translation="MKNLLIAVATVWTTLAATGGYAADVVRETRAVSGFSRIEVEGQA
                     DITLRQGTTEALTLEATAQGLKQILTEVRGRTLRISLGGQSHWWQWTPGGSATRTPRI
                     AIDLIQIERIEAAGAVNIVADTLKANELRLDFAGACKLKIGDLQATRLRLDGAGAIKA
                     EIAGKVAEQSIDLSGAGSYLAVDLISDNVAVQVSGAGKAIVNAKTLLKADISGAGLVE
                     YTGNPRLEQEISGIGKIRRR"
     gene            complement(1300..1677)
                     /locus_tag="VHT22_08380"
     CDS             complement(1300..1677)
                     /locus_tag="VHT22_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3137612.1"
                     /translation="MRKLHGNTDPAERCHITLAEAAPLLPAPDGKRSVALFEHGTLQV
                     KLYAPRGVDPQSPHTRDEIYVIARGTGWFVNGEARHRFAPDDVMFVPAGIVHRFEEFS
                     NDFAVWVFFYGPEGGELQEPERA"
     gene            complement(1684..2502)
                     /locus_tag="VHT22_08385"
     CDS             complement(1684..2502)
                     /locus_tag="VHT22_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX3137613.1"
                     /translation="MYKNILVATDGTRLSGKAITHAIELAKALGGSETFALASPLVTS
                     DAFDLARLPQLLAWQRQAADGGVDHLDLHPTPIATKVGQEGVHLRFLAGVALAAPQAD
                     LLRETESAAGAMALAQELAHQLAGPGYSVLALPRAPQSPLAALQQGRAAQRDIGAQLF
                     ASNAIRRLRASVGEPSAVISAHRCVGAPGGGELRLSLSSPFDPRRAEGFRCPLFAGDR
                     AVDVARMLADLMRDCRVTDVQALPGVHADRDSATGLTLLFKADAVAAARQSVVH"
BASE COUNT          467 a          836 c          864 g          385 t
ORIGIN      
        1 cggacattat gtcgcgccga acgccaccat catcggcgac gtcgtgctcg agagcgagtc
       61 gtccatctgg ttcaacgtcg tcatccgcgg tgacaacgac acgatcacga tcggagcgcg
      121 gtcgaatgtc caggacgggt cggtgctgca caccgacgcc ggcgtgcccc tgaccctggg
      181 ctcgtcggtt tcggtcggcc acctggtgat gctgcacggg tgcacgatcg gcgatggctc
      241 gctgatcggc atcaaggcgg tgatcctcaa cggcgcccgg ataggccgcg actgcctgat
      301 cggcgccaat acgctgatcg ccgagggcaa gacgattccc gaccgctcgc tggtgctggg
      361 ctcgccgggc agggtaatgc gcacgctcga tgacggcgag gtcgccggga tccgcgcgat
      421 cgccgatcat tatgtcgaca acgcccgccg ctatcgggcg gatttcaagc cgatttgacg
      481 ggccgccagc gaaaatcatg aatccgccgt cgccggccag cacatccaag cggtattgcc
      541 gccattggga agtcgcgtga aaaacctgct gatcgctgtt gccactgtct ggacgacgct
      601 cgccgcgact ggcggctacg ccgctgacgt cgtccgggaa acgcgggccg taagcggctt
      661 ttcgcggatc gaagtcgaag ggcaagccga tatcacgctg cggcaaggga cgaccgaggc
      721 ccttacgctg gaggcaacgg cacaaggcct taagcagatt ctcaccgagg tgcgcggccg
      781 aacgctgcgt atcagcctgg gcggacaaag ccactggtgg caatggacgc ccgggggcag
      841 cgccacgcgc acgccgcgga tcgcgatcga cctgatccag atcgagcgca tcgaggcggc
      901 cggcgcggtc aacatcgtcg ccgacaccct gaaggccaac gagctgcgac tcgatttcgc
      961 cggcgcctgc aagctcaaga ttggcgactt gcaggcaacc aggctgcgtc tggacggtgc
     1021 gggcgcgatc aaggccgaga tcgccggcaa ggtggccgag cagagcatcg acctgtcggg
     1081 cgccggatcg tatctggccg tcgaccttat cagcgacaac gtcgcggtcc aggtcagcgg
     1141 tgcgggcaag gcgatcgtca acgccaagac gctgctcaag gcggacatct cgggcgcggg
     1201 actggtcgaa tacaccggca atccacggct cgagcaggaa atcagcggca tcggcaaaat
     1261 caggcgccgg tagccggaaa ccgcaccggc gccgacgctc taagcgcgct caggttcctg
     1321 aagctccccg ccttcggggc cgtagaagaa cacccacacc gcgaagtcgt tcgaaaattc
     1381 ctcgaaacga tgcacgatgc ccgccggcac aaacatcacg tcgtcgggcg cgaagcgatg
     1441 acgagcctcg ccattgacga accatccggt gccgcgcgca atgacataga tttcatcgcg
     1501 cgtgtgcggc gactgcgggt cgactccgcg cggcgcgtag agcttcacct gcaatgtccc
     1561 gtgctcgaac agcgccacgg agcgcttgcc gtccggtgcg ggcagcagcg gcgccgcctc
     1621 cgccagcgtg atgtggcagc gctctgccgg atcagtattg ccgtggagct tgcgcatcgc
     1681 tgatcaatgc acgaccgact gacgcgccgc ggcgacagcg tcggccttga acagcagggt
     1741 cagaccggtt gccgaatcgc gatcggcgtg caccccgggg agcgcctgca cgtcggtgac
     1801 gcggcaatca cgcatcaggt cggcgagcat tctcgccacg tcgacggcgc gatcgccggc
     1861 aaacaacgga cagcgaaagc cttcggcccg tctcggatcg aacgggctgg acagcgacag
     1921 gcgcaattcg ccgccgcccg gcgcgccgac gcagcggtgc gcactgatca ccgccgacgg
     1981 ctcgccgacg ctggcgcgca gtcggcgaat cgcgttgctc gcgaaaagct gcgccccgat
     2041 gtcgcgctgc gctgcgcgcc cctgctgcag cgcagccaga ggcgactgtg gtgcgcgagg
     2101 cagcgcgagc accgaatagc cggggccggc aagctggtgc gccagttcct gcgcgagcgc
     2161 catcgcaccg gcagcgcttt cggtttcgcg cagcagatcg gcctgcggtg cggccagtgc
     2221 gacgccggcg agaaagcgca agtgcacgcc ttcctgcccg accttggtcg caatcggcgt
     2281 cggatgcaga tcgagatgat cgacgccacc gtcggcagcc tgccgttgcc aggcgaggag
     2341 ctgcggcagg cgcgccaagt cgaatgcatc ggacgtgacc agcggtgagg cgagggcgaa
     2401 tgtctcgctg ccgccgagcg ccttggccag ctcgatggca tgggtgatgg ccttgccgga
     2461 aagccgcgtg ccgtcggtcg cgacaagaat gttcttgtac attgtggggc ctcctgtcga
     2521 aaatgcgccc cgcgtgcagg taggttgcgg ta
//
LOCUS       DATANS010000571         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_365388, whole genome shotgun sequence.
ACCESSION   DATANS010000571 DATANS010000000
VERSION     DATANS010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365388"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VHT22_08390"
     CDS             complement(<1..514)
                     /locus_tag="VHT22_08390"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3137614.1"
                     /translation="MIPTGGGALPGDAYQQRIAAERENFDACEEVHALPPIYHYWSNR
                     YLRPRLEMFGVSHPDAFFAKYLADCYGRGSGTRRFVSIGAGHCDTEVRLARALIDAGC
                     DDFAIECLELNDTMLERGRQLAISEGLADKVVPSQADFNEWQPVGWYDGVIANQSLHH
                     VVALEHLFDVV"
     gene            complement(627..1463)
                     /locus_tag="VHT22_08395"
     CDS             complement(627..1463)
                     /locus_tag="VHT22_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3137615.1"
                     /translation="MSARHRALHWLRTLAGKPVGRDRPSADAADLVAFRCNICGAACR
                     VAPALLSRETPSCITCGSTVRMRAIVHLLACELFGRSMALPDLPHRPDLIGIGLSDSP
                     AYAVPLAKKLGYTNTYFHTEPRLDIAAVPERRAAEADFIIASDVFEHVAPPVARAFSN
                     ARRLLKPGGVLIFSVPFSLAADTVEHFPALHDYRLIDAGGSWRLENRTSDGREEVFTD
                     LVFHGGPGTTLEMRLFSRAGLERAFTEAGFARMRIADEACPEHGIVWPEPWSVPMVAY
                     AP"
     gene            complement(1503..>1648)
                     /locus_tag="VHT22_08400"
     CDS             complement(1503..>1648)
                     /locus_tag="VHT22_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX3137616.1"
                     /translation="THLLNHGADLRVVQLLLGHSDITTTTIYTHVARERLKQLHHRHH
                     PRG"
BASE COUNT          312 a          530 c          559 g          247 t
ORIGIN      
        1 cgacgacatc gaacagatgc tcgagcgcga ccacgtggtg cagcgactgg ttggcgatga
       61 ccccgtcgta ccatccgacc ggctgccatt cgttgaagtc cgcctgcgac ggaacgacct
      121 tgtcggcgag gccttcgctg atcgccagct gcctgccccg ctccagcatc gtgtcgttca
      181 gttcgagaca ttcgatcgca aaatcatcgc agcccgcgtc gatcaatgcg cgcgccaggc
      241 ggacctcggt atcgcaatgg ccggcgccga tgctgacaaa gcgtcgtgtg ccgctgccgc
      301 ggccgtagca atcggccaga tatttggcaa agaaggcgtc ggggtggctt acgccaaaca
      361 tttcgaggcg cggtcgcaga taacgattgg accagtagtg gtatatcggc gggagggcgt
      421 ggacttcttc gcacgcatcg aaattctcgc gctctgcggc aatgcgctgc tgataagcgt
      481 ccccgggaag cgcgccgccg ccggttggaa tcacgtctct tgagcgagcg acagacatcg
      541 gcgacaacgc gcgcctgaga cgatcgcgaa cccgcccggc gagtgccctc gtacgcgtac
      601 cgatcgccgc tctgcgcgaa gcgccgtcag ggcgcatacg ccaccatcgg caccgaccac
      661 ggctccggcc agacgatgcc atgctcggga caagcctcgt cggcaatgcg catgcgcgcg
      721 aacccggcct cggtgaatgc ccgctccagc ccggcgcgtg agaaaagccg catctccagc
      781 gtcgtgccgg gcccgccgtg aaagaccaga tcggtgaaca cttcctcgcg tccgtccgag
      841 gtgcggtttt ccagccgcca agagccgccg gcgtcgatca agcgatagtc gtgcagcgcc
      901 gggaagtgct cgacggtgtc ggcggccagc gaaaacggca ccgaaaaaat cagcacgccg
      961 ccgggcttca gaagccgccg tgcattggaa aacgcacgcg ccaccggcgg cgccacgtgc
     1021 tcgaagacgt cgctcgcgat gatgaagtcc gcctcggcag cacgacgctc gggcacagcg
     1081 gcgatgtcga gccgaggctc ggtatggaaa taggtgttgg tatagccgag cttcttcgcc
     1141 agcggcaccg catacgcggg cgagtcggac agcccgatgc cgatcaggtc tggccggtgc
     1201 ggcagatcgg gcagggccat gctgcggccg aagagctcgc aggccagaag atggacgatg
     1261 gcccgcattc gcaccgtcga gccgcaggtg atgcacgacg gcgtctcgcg cgaaagcaac
     1321 gcgggggcga cacggcaggc cgcgccgcaa atattgcagc gaaacgcgac caggtcggcc
     1381 gcgtcggcgg aaggccgatc cctgcccacc ggcttgccag cgagggtgcg cagccagtgc
     1441 aaggctcggt ggcgagcgga catcggatgg gcgcgctgtc ggaaatgcga gcgatgcggc
     1501 gatcagccgc gcggatggtg gcggtggtgc agctgcttca accgctcgcg cgccacatgg
     1561 gtgtagatcg tcgtcgtcgt gatgtccgaa tgcccgagca gcaactgcac gacacggaga
     1621 tcggcgccgt ggttcagaag atgcgtgg
//
LOCUS       DATANS010000572         1587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_784095, whole genome shotgun sequence.
ACCESSION   DATANS010000572 DATANS010000000
VERSION     DATANS010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784095"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VHT22_08405"
     CDS             complement(<1..409)
                     /locus_tag="VHT22_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX3137617.1"
                     /translation="MSAPNPFPNPALRAAQQGGQRRREKRDVHGWIVLDKPVGMTSTH
                     AVSVIKRLFTAKRAGHAGTLDPLASGLLPLALGEATKTVPFVMDGRKRYRFTVRWGEE
                     RDTDDAEGRVVATSDQRPTAEAIRGCLPAFTGTI"
     gene            complement(406..819)
                     /gene="rbfA"
                     /locus_tag="VHT22_08410"
     CDS             complement(406..819)
                     /gene="rbfA"
                     /locus_tag="VHT22_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX3137618.1"
                     /translation="MPRHHHRDHSGPSQRQLRVGELVRHALAEMLSRGEVHDPVIEGH
                     MITVPEVRMSPDLRLATIYVMPLGGKDEQEVVAALERNKKFLRGEIAHRVNLKFAPEI
                     RFRVDERFDEAERIEKILRTPEVRRDLESKPDDES"
     gene            complement(940..>1587)
                     /locus_tag="VHT22_08415"
     CDS             complement(940..>1587)
                     /locus_tag="VHT22_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEX3137619.1"
                     /translation="EQMMAQVKTAGRKDFPLIIKGDVQGSIEAIAGALEKIGNDEVGA
                     RIIHSGVGGITESDVTLAESSGAAIIGFNVRAHKEAREAAERTGTEIRYYNIIYNLVD
                     DVKKAMSGLLTPELRETMLGNAQILEIFVVSKVGKIAGCRVTDGKVERGANVRLIRDN
                     VVIHEGKLSQLKRFKDDAREVLAGQECGMAFENYQDMRQGDVIECYRVETVQRSL"
BASE COUNT          251 a          511 c          517 g          308 t
ORIGIN      
        1 cgatcgtgcc ggtgaaggcc gggagacagc cgcggatcgc ctcggcggtc ggacgctggt
       61 cggaggtcgc cacgacgcgg ccttccgcat cgtcagtgtc gcgctcctcg ccccagcgca
      121 ccgtgaaacg atagcgcttg cggccgtcca tcacgaacgg gaccgtcttg gtagcctctc
      181 cgagcgcgag cggcaggagg ccggaggcga gcggatcgag cgtgccggcg tggccggcgc
      241 gcttggcggt gaacagccgc ttgatgaccg agaccgcgtg cgtagaggtc atgccgaccg
      301 gcttgtcgag cacgatccag ccgtgcacgt cgcgcttctc gcgccggcgc tggccgccct
      361 gttgcgccgc ccggagcgcc gggttgggaa acggattcgg cgcgctcatg attcgtcgtc
      421 cggctttgat tcgagatcgc gccggacctc aggcgttcgc aaaatcttct cgatgcgttc
      481 tgcttcgtcg aatcgctcgt cgacgcggaa gcgaatttca ggtgcaaact tcaggttaac
      541 gcgatgcgcg atctcgccgc gcaggaattt cttgttgcgc tcaagcgccg cgacaacttc
      601 ttgctcgtcc ttgccgccga gcggcatcac gtagatcgtc gcgagccgca ggtccgggct
      661 catgcgcact tccggcacgg tgatcatgtg gccctcgatc acgggatcat gcacctcgcc
      721 gcgcgagagc atctcggcga gcgcgtggcg aacgagctcg ccgacacgca gctggcgctg
      781 cgagggaccg gaatggtcgc ggtgatggtg gcgaggcatt aacaaagtcc tgaaatatgc
      841 gctccgtcat gcccggcctt gtgccgggca tccacgtctt aacagcagtg caaaaagtgt
      901 aagacgtgga tagccgggac aagcccggcc atgactctgt tatagcgacc gctgcaccgt
      961 ctccacgcga tagcactcga tcacgtcgcc ctggcgcatg tcctggtagt tctcgaacgc
     1021 catgccgcat tcctggccgg ccagcacctc gcgcgcatcg tccttgaagc gcttgagctg
     1081 tgagagcttg ccctcgtgga tcacgacgtt gtcgcggatc aggcgcacgt tcgcgccgcg
     1141 ctcgaccttg ccgtcggtga cgcggcagcc ggcgatcttg ccgactttgg agacgacgaa
     1201 gatctccagg atctgcgcat tgcccagcat ggtctcgcgc agttccggcg tgagcaggcc
     1261 ggacatcgcc ttcttcacgt catccacgag attgtagatg atgttgtagt agcggatctc
     1321 cgtgccggtg cgctcggccg cctcgcgcgc ttccttgtgg gcgcgcacgt tgaagccgat
     1381 gattgcggcg ccggaggatt cggcgagcgt cacgtcggat tcggtgatgc cgccgacgcc
     1441 cgaatggatg attcgcgcgc cgacttcgtc gttgccgatc ttttccaggg cacccgcgat
     1501 ggcctcgatc gagccctgca cgtcgccctt gatgatgagc gggaaatcct tgcggcccgc
     1561 ggtcttgacc tgggccatca tctgttc
//
LOCUS       DATANS010000573         1698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_614357, whole genome shotgun sequence.
ACCESSION   DATANS010000573 DATANS010000000
VERSION     DATANS010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614357"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..880)
                     /locus_tag="VHT22_08420"
     CDS             complement(182..880)
                     /locus_tag="VHT22_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX3137620.1"
                     /translation="MIDLYYWPTPNGHKMTMFLEEAKLEYKIHPVNIGKGEQFKPEFL
                     SIAPNNRIPAIVDRAPADGGEPIAMFESGAILLYLADKVGRFVPRDLRARLDCIQWLF
                     WQMANLGPLAGQNHHFVHYAPERLQYAIDRYVNETGRLYAVLNKRLADREFIVGDYSI
                     ADVASYPWVLPERQSQNIDEFPHLKRWKEAIKARPATERAYALGKEINPKPAATRTDE
                     ERKILFGQTKDVVR"
     gene            complement(950..>1698)
                     /locus_tag="VHT22_08425"
     CDS             complement(950..>1698)
                     /locus_tag="VHT22_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3137621.1"
                     /translation="VCAASKGLGRGCAEALAREGVDVTIVARTEATLRQAAEDIGATA
                     GRAVRWVACDITTAEGRGKALAACPQPDILVNNAGGPPPGDFRDWDRDAWLRAIDANM
                     LTPIELIKATVDGMIARNFGRIVNITSSAVKAPIDILGLSNGARSGLTGFVAGLARKV
                     ARHNVTINNLLPGPFDTDRLRANMELRARTAGQPLAELRQAQMDANPSGRFGTPAEFG
                     IACAFLCSVHAGYIVGQNLLIDGGGYPGTF"
BASE COUNT          268 a          538 c          565 g          327 t
ORIGIN      
        1 gccacggcaa cccgtttcgc gcggttgccg gcacgcctta tctggcggaa ggggaagcag
       61 ccgtcgtgcg gcccttaggc gagcgcgggt ttcgcgtgat cgctcgcatt cccaaagacg
      121 gctggagcgc gctcgaaacg ccgtaggcgt caaggtgctc tgcgcaaaga cgccggtcga
      181 ttcagcgcac gacgtccttg gtctgcccga acaggatctt tctctcctcg tcggtgcgtg
      241 tcgccgcggg tttcggattg atttccttgc ccagtgcgta ggcgcgctcc gtcgcgggac
      301 gcgccttgat cgcctccttc caccgtttca ggtgcgggaa ctcgtcgatg ttctgtgact
      361 ggcgctctgg cagcacccac ggatacgacg cgacgtcggc gatcgaatag tcgccgacga
      421 tgaattcgcg atcggccagc cgcttgttca gcaccgcgta cagcctgccc gtttcgttga
      481 cgtagcgatc gatggcgtac tggagccgtt ccggcgcata gtggacgaaa tgatggttct
      541 ggccggcgag cggccccagg ttcgccatct gccagaacag ccactggatg caatccagcc
      601 gcgcgcgcag atcgcgcggg acgaagcgtc cgaccttgtc ggcgaggtag agcaggatcg
      661 cgcccgattc gaacatcgcg atcggttccc cgccgtcggc aggagcgcga tcgacgatcg
      721 ccgggatgcg gttgttcggc gcgatagaca ggaattccgg cttgaactgc tcgcctttgc
      781 cgatgttcac cggatggatc ttgtactcga gcttcgcttc ctcgagaaac atcgtcatct
      841 tgtggccgtt cggcgtcggc cagtaataga gatcgatcat cgtgtcgttg gaatcatttc
      901 gagtgggtga tgacgatggc gcaatagagc tcgatgatag cgtggggcgc tagaaagttc
      961 caggataccc gccgccgtcg atcagcaggt tctggccgac gatgtagccc gcgtgcacgc
     1021 tgcacagaaa tgcgcaggcg atgccgaact cggccggcgt gccgaatcgt ccggaggggt
     1081 tggcgtccat ttgcgcctgg cgcagctccg ccagcggctg gcctgccgtc ctggcgcgca
     1141 attccatgtt ggcgcgcagg cgatcggtgt cgaacgggcc cggcagcaga ttgttgatgg
     1201 tcacattgtg ccgcgcgacc ttgcgcgcga gtcccgcgac gaagcccgtc aggccgctgc
     1261 gcgcgccgtt cgacagtccg aggatgtcga tcggtgcctt caccgcagac gaggtgatgt
     1321 tgacaatgcg gccgaagtta cgcgcgatca tgccgtcgac ggtcgccttg atcagctcga
     1381 tcggcgtcag catgttggca tcgatcgcgc gcagccacgc gtcgcggtcc cagtcgcgaa
     1441 agtcgccagg cggtgggccg ccggcattgt tgaccaggat gtccggttgc gggcacgccg
     1501 ccagcgcttt gccgcggcct tcggcggtcg taatatcgca cgcgacccag cgcacggcgc
     1561 ggcccgccgt cgcgccgatg tcttcggcgg cctgacgcag tgtggcttcg gtgcgcgcga
     1621 cgatcgtcac gtctacgcct tcgcgcgcca gcgcttcagc gcagccgcgt ccgagaccct
     1681 tgctcgcggc gcagacca
//
LOCUS       DATANS010000574         2700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_492067, whole genome shotgun sequence.
ACCESSION   DATANS010000574 DATANS010000000
VERSION     DATANS010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492067"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="VHT22_08430"
     CDS             complement(<1..175)
                     /locus_tag="VHT22_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX3137622.1"
                     /translation="MANAPEVTNMALFCDFENVALGVQDAKYERFDIGKVLERLLLKG
                     SIVVKKAYCDWDRY"
     gene            338..889
                     /locus_tag="VHT22_08435"
     CDS             338..889
                     /locus_tag="VHT22_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX3137623.1"
                     /translation="MLRRLLLVVALLPAAAFAAGSFTLSSAEIKPGATIAEAQVFKGF
                     GCEGGNVSPSLAWKNAPAGTKSFALTVYDPDAPTGSGWWHWVMFNIPADVNALPAGAG
                     DSAGGKAPRGAIQSRTDFGKPGYGGPCPPNGDRPHRYIFTIHALKVDHIDADQDASGA
                     LAGFMINANQIGKATFTGKYGRK"
     gene            912..1571
                     /locus_tag="VHT22_08440"
     CDS             912..1571
                     /locus_tag="VHT22_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HEX3137624.1"
                     /translation="MSAAVPTLSLLEARILGVLVEKQHTVPDIYPLSLNALTAGCNQK
                     TSRDPILHASESEVQAGIDRLRGDSLVVESSGGRVMRYAHNVERVLALPSQAVALLAT
                     MMLRGPQTIGELRINSDRLHRFADGSAVEGFLNELAERSAGALVVMLPRLPGARENRW
                     MHLLSGTPADVPADIDSAAAPPRDDTLRTEISALKRSVSELRDEIESLKAAISHLNRD
                     A"
     gene            1732..>2700
                     /locus_tag="VHT22_08445"
     CDS             1732..>2700
                     /locus_tag="VHT22_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3137625.1"
                     /translation="MRLTGILSLVFGAIALAASAQAQVPAGYPADYKAIVDGAKKEGK
                     VVVYSTTDAKLVTPLIKDFEAAFPGVKVEYTDLNSTEVYNRYISESAANAASADAVWS
                     SSMDLQMKLAADGLAAPYKSPEAGALPPWAHWKDTIYATTFEPIAIVYNKRLVPPEEV
                     PKTHADLVKLLNTKGDKYQNKITAYDPEKSGVGFMLANQDAKLDPQFWELVKALGARS
                     LKVQSSTGTMLERVSSGENLLGYNVLGSYALTRAKKDPSLGVAFTTDYNLVLSRLIFV
                     SKNARNPNAGRLFLDYVLSKRGQTIAAEQAELYSVRTDMTGKDSGVG"
BASE COUNT          519 a          866 c          863 g          452 t
ORIGIN      
        1 tgtagcggtc ccagtcgcag taggcctttt tgacgacgat gctgcctttg agcagcaggc
       61 gctcgagcac tttgccgatg tcgaaccgct cgtacttggc gtcctggacg ccgagcgcga
      121 cgttctcgaa atcgcagaac agcgccatgt tggtgacttc gggagcgttg gccatgagga
      181 tcttggaaaa tggctggcga gcgaggctcg ctcaaccggg catcatagcg caacgaccgc
      241 cgtgcgggca ccttcgaagc gatgccgcgc attccgcaac cggacgaggc cggtttagac
      301 ttacggtttg cgcccaaacc cgcccgaagg aggcactatg cttcgccgct tgctgttggt
      361 cgttgcgctg ctgccggctg ccgcatttgc cgccggatcg ttcacgcttt cgagcgccga
      421 aatcaagccg ggcgcgacca tagccgaagc gcaagtgttc aaggggttcg gctgcgaggg
      481 aggcaacgtt tcgccgtcgc tggcatggaa gaatgcgccc gcgggcacga aaagcttcgc
      541 gttgaccgtc tacgatcccg acgctccgac gggcagcggc tggtggcatt gggtgatgtt
      601 caacatcccc gccgacgtca acgcgcttcc cgctggcgcc ggcgattccg ctggcggcaa
      661 ggcgccgcgg ggcgccatcc agtcgcgcac cgatttcggc aagcccggct atggcggccc
      721 ctgtccaccg aacggcgacc ggccgcaccg ctacattttc acgatccacg cgttgaaggt
      781 cgatcatatc gacgccgatc aggacgcgag cggagccctg gcgggcttca tgatcaacgc
      841 caatcagatc ggcaaggcga cattcaccgg aaaatacggc cgcaagtgac aacgggcaga
      901 cgagcgaagc gatgagcgcg gccgtcccga cactgtcgct gctcgaggcg cgcattctcg
      961 gcgtgctggt cgaaaagcaa cacacggtgc cggacatcta tccgctcagc ctcaatgcgc
     1021 tgactgccgg atgcaatcag aaaaccagcc gcgatccgat attgcacgcc agcgagagtg
     1081 aggttcaggc cggcatcgac cggctgcgcg gcgactcgct ggtcgtcgaa tcgagcggtg
     1141 gccgggtgat gcgctacgcg cacaacgtcg agcgcgtgct ggcactgccg tcgcaggcgg
     1201 tggcgctgct cgccacgatg atgctgcgcg gaccgcaaac catcggcgag ctccgcatca
     1261 attccgatcg cctgcaccgg ttcgccgacg gctcggccgt cgaaggtttc ctgaacgagc
     1321 tcgcggagcg cagtgccgga gcgctggtcg tcatgctgcc aaggcttccc ggcgcgcgcg
     1381 aaaatcgctg gatgcacctg ttgtccggga ctcccgccga cgtgcccgcg gacatcgatt
     1441 cggccgcggc gccgccgcgt gacgacacgc tgcggacgga aatctccgcg ctcaagcgaa
     1501 gcgtcagcga gctccgtgac gagatcgaat cgctgaaggc ggcgatttcg caccttaatc
     1561 gcgacgcctg aaagcgacga tcatcgaacg tgcggccgtc aggcggccgg acgatccgac
     1621 gctgtcgccg cccggcccaa ccgccggccc ggaacggtgt aaactggccg ccagagccgc
     1681 tcgccgacgt tcctggcggc acgccttgac cgataccaga aggagttcgc aatgcgcttg
     1741 accgggattt tgtcgctcgt ctttggcgcg attgcgctgg cagcaagcgc gcaagcgcag
     1801 gtgcccgccg gctatccggc cgattacaaa gccatcgttg acggcgccaa aaaggaaggc
     1861 aaggtcgtcg tctactcgac caccgacgcc aagctggtca cgccgctgat caaggatttc
     1921 gaggcggcgt ttccaggcgt gaaggtcgag tacaccgacc tgaactcgac cgaggtctat
     1981 aaccggtaca tcagtgaaag cgccgccaac gcggcttcgg ccgacgccgt gtggagctca
     2041 tcgatggacc tgcagatgaa gctggccgcc gatggcctgg cagcgcccta caagtcgccg
     2101 gaagcgggcg cgttgccgcc atgggcgcac tggaaagaca cgatctatgc gacgacgttc
     2161 gagcccatcg cgatcgtgta caacaagcgt ctcgttccgc ccgaagaagt gccgaaaacg
     2221 cacgccgacc tggtcaagct gctgaacacc aagggcgaca agtatcagaa caagatcacc
     2281 gcgtacgatc cggaaaagtc gggtgtgggc ttcatgctcg ccaaccagga cgccaagctc
     2341 gatccgcaat tctgggagct ggtcaaggcg ctgggtgcgc gcagtctcaa ggtgcagtcg
     2401 tcgaccggta ccatgctcga gcgcgtttcc tccggcgaga atctgctcgg ctacaacgtg
     2461 ctcggctcgt atgctctgac gcgggcgaaa aaagacccgt cgctcggcgt cgcgttcacc
     2521 accgattaca acctggtgct gtcgcggctg atcttcgtgt cgaagaacgc caggaacccg
     2581 aacgccggcc ggctgtttct cgattacgtg ctgtccaagc gcggacagac gatcgccgct
     2641 gaacaggccg agctttattc ggtgcgcacc gacatgaccg gcaaggactc gggcgtcggg
//
LOCUS       DATANS010000575         3568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_784124, whole genome shotgun sequence.
ACCESSION   DATANS010000575 DATANS010000000
VERSION     DATANS010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784124"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VHT22_08450"
     CDS             <1..440
                     /locus_tag="VHT22_08450"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEX3137626.1"
                     /translation="YAASLGAFLTIYACYLVLRRKEHVVRGNAWHDALAGGLGGIAGG
                     LAGFPGAFVTIWCSMRGWDKLKQRAVYQPYILAMQIVTIACLRWQSPIQEGIAQDLSF
                     VPFALIGAVGGLAVFQRLSQKQFQVAVSLLLAVSGVGLLVRGL"
     gene            538..1200
                     /locus_tag="VHT22_08455"
     CDS             538..1200
                     /locus_tag="VHT22_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137627.1"
                     /translation="MGKTKVVAFGTLVGVALALAPLSSAFADTRSNRHSGHGGNWGGG
                     HGHASYAVHGYGYGGHGCYGCLWPFGLAAAVVTATAAVVTLPFVALNAAVNAPYYASP
                     AGYGPPAAYGPPAASDSTLPSNYNNAPAAYYGPPARYYNRAPAPVSYGPPAAPAYYRT
                     APAYAPPARYYAQPAQNYAPPAQSYAPPAAPAAPAYNYGPPPDYYNQDSPAAPNYYDY
                     QR"
     gene            complement(1345..1503)
                     /locus_tag="VHT22_08460"
     CDS             complement(1345..1503)
                     /locus_tag="VHT22_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137628.1"
                     /translation="MDDGDKKRRLIGEAMVERALGNSGASRYRFDARGAVAVRQEKMR
                     GGVKNPLA"
     gene            1543..1935
                     /locus_tag="VHT22_08465"
     CDS             1543..1935
                     /locus_tag="VHT22_08465"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEX3137629.1"
                     /translation="MLEGDLREILRRLAGLILHAALSPQAIALNRMIVAESGRFPQLA
                     AVTTGATEEAIRLIVGILEGEVGAKRLALANPTFAAQQFLYMVIALPQRRAMGFGAPM
                     PSAEIDAWAGDVVNLFLNGCRGQPRAAR"
     gene            1980..2942
                     /locus_tag="VHT22_08470"
     CDS             1980..2942
                     /locus_tag="VHT22_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX3137630.1"
                     /translation="MRALRFHTYGPPDVLRIDEVPEPSPEAGQVKVRVHAGCLNPIDW
                     KIRAGHVRLVPLFRSPPRGVGTDFAGEIVTLGAGPSARRVGERVFGSLQQFGREGALA
                     DFIVVSDERVLPLPDNVDFAQAAALPVAAGTALQALTDEAHVGPGQRVLITGAAGGVG
                     HFAVQIAKHLGAYVIGVCSEANTAFVRELGADDVVDYAHDDFTRRSDRFDVVFDAACA
                     SNFVAASNVMTPSGVYINTSGTVAALLGTAASAVLARFTSRQRAIPFTLRHDARIWQR
                     ALQLVRQNALKAHIERTITLEEVADAQRAMETGHGRGKIVVRLR"
     gene            3063..>3568
                     /locus_tag="VHT22_08475"
     CDS             3063..>3568
                     /locus_tag="VHT22_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEX3137631.1"
                     /translation="MANVPQWQAEGDWFDVCRCDIPCPCEFAQPPTDNECEGILVWHV
                     RKGRYGDVPIDGLNVVALGHFEGNIWAGAKATMGIILDERGDPKQREALQMIFGGQAG
                     GWPGQFAQTVAEVRGIEFARIEVDIAGDLAHWSASVAGRVEAKAEALTGPTTPAGKRV
                     QTVNPPGS"
BASE COUNT          553 a         1219 c         1173 g          623 t
ORIGIN      
        1 tctacgcggc aagcctcggc gcgttcctga cgatatacgc ctgctacctc gtgctgcgcc
       61 ggaaggaaca tgtcgtgcgc ggcaacgcgt ggcacgatgc gctcgccggt gggctgggag
      121 gaatcgccgg cggcctcgca ggtttccccg gcgcattcgt gacgatctgg tgctcgatgc
      181 gcggctggga caagctgaag cagcgtgccg tctaccagcc gtacatcctg gcgatgcaga
      241 tcgtgaccat cgcgtgcctg cgctggcagt cgccgatcca ggaaggcatc gcgcaggatc
      301 tcagcttcgt gccgtttgcg ctgatcggcg ccgtcggcgg tctcgcggtg ttccagcggc
      361 tgagccagaa gcaattccag gtggcggtga gcctgctgct tgcagtgtcg ggggtcgggc
      421 tgctggtgcg gggactctaa tgtaacaata cgctacggtt gcgttgcgtt atgacggaac
      481 gtgctagcct gggtccaatc ggatgcgacc tgtccgcgcc acgacgagga gcaagacatg
      541 ggcaagacca aagttgttgc gttcggcact ctcgttggcg ttgctctcgc cctcgcgccg
      601 ctgtcgtccg cctttgccga cactcgatcc aaccggcatt cgggtcacgg cggtaattgg
      661 ggcggcggcc acggccacgc gagttacgcg gtccacggct acggctacgg cggtcacggt
      721 tgctacggct gcttgtggcc gttcggcttg gcggctgcgg tcgtcaccgc caccgctgca
      781 gtcgtcacac tgccgttcgt cgctctcaac gctgccgtga acgcaccgta ctacgcatcg
      841 cccgcgggat atggcccgcc tgcggcttat ggcccccccg ccgccagcga ctcgacgctg
      901 ccttccaact acaacaacgc gccggctgcc tattacgggc cacccgcgcg ctactataac
      961 cgtgcgcccg ctccggtctc ctacggtccg cccgcagcgc cggcttatta ccgcactgca
     1021 cctgcctacg cgccgcccgc gcgctattac gctcagcccg cacagaatta cgctccgcct
     1081 gcgcagtctt acgctccgcc tgcggctccg gcggcgccgg cttacaacta cgggccgccg
     1141 ccggactact acaaccaaga ttcccctgct gctcccaact actacgacta tcagcgctga
     1201 tcgcgtcggc ggatgaggcg atcggccggg ggcggttcga caccgcgagt tcgagctaag
     1261 aagacggcgt ccgcaacgcg tgccgccgca cttgccacgg ctgccggccg cggcggtcgc
     1321 ccttcccggc agcacgcggc acagctaggc gagcggattc ttaacgccgc cacgcatctt
     1381 ttcctgtcgc acggctacgg cgccacgagc atcgaagcgg tagcgcgacg cgcccgaatt
     1441 tccaagcgca cgttctacca tcgcttcccc gataagcctg cgctttttgt cgccgtcgtc
     1501 catcgcatca tcgaaggcct gcgtccgccc gccgatatac cgttgctcga aggcgacctg
     1561 cgtgagatcc tgcggcggct cgcaggactc atcctgcacg cggcgctgtc gccacaggcg
     1621 atcgctttga atcgaatgat cgtcgcggaa tcgggaagat ttccgcagct ggccgctgtc
     1681 acgaccggcg ctaccgagga ggcgatccgg ctcatcgtcg gcattctcga aggtgaagtc
     1741 ggcgccaaac ggctcgcatt ggccaacccg acattcgccg cccagcagtt tctttacatg
     1801 gtcatcgcgc tgccgcagcg ccgggccatg ggcttcggcg ccccaatgcc ctcagccgag
     1861 atcgatgcct gggcgggcga cgtcgtgaat ctgttcctga atggctgtcg cggccagccg
     1921 cgcgcggcgc gctgattcgt atcgtgcgtt gtgcgggccg aggtattact atcggctaca
     1981 tgcgcgcgct tcgattccac acctatggtc cgccggacgt cctgcgcatc gacgaggtgc
     2041 ccgagccctc gcccgaggcg ggccaggtca aggtgcgcgt gcacgcgggc tgcctcaacc
     2101 cgatcgactg gaagattcgc gccgggcatg tgcgcctggt gccgctcttc cgctcgccgc
     2161 cgcgcggagt gggcaccgat ttcgccgggg agatcgtcac cctcggcgcc ggccccagtg
     2221 cgcgccgggt cggcgagcgc gtcttcggct cgctgcagca gttcggacgc gagggcgccc
     2281 ttgccgactt tatcgtggtg tccgacgagc gcgtgctgcc gcttccggac aatgtggact
     2341 tcgcgcaggc ggcggcgctg ccggtcgccg caggcactgc gctgcaggcg ctgactgacg
     2401 aggcgcacgt cggcccggga cagcgcgtgc tgatcaccgg cgctgccggc ggcgtcgggc
     2461 acttcgccgt gcagatcgcc aagcatctgg gtgcgtacgt catcggcgtc tgcagtgaag
     2521 cgaataccgc attcgtgcgc gagctaggcg ctgacgacgt tgtcgattac gcccacgatg
     2581 acttcacgcg ccgcagcgac cgcttcgacg tcgttttcga cgccgcctgc gcgtcgaact
     2641 ttgtcgctgc gagcaacgtg atgacgccgt cgggcgtcta catcaacacc agcggtaccg
     2701 tcgccgccct gctcggcact gcggcgtcgg ccgtgttagc ccgattcacg tcgcgccagc
     2761 gcgcaattcc gttcaccttg cgccacgacg cccgcatctg gcagcgagcg ctgcagctcg
     2821 tgcgccaaaa tgcgctcaag gcgcacatcg agcgcacgat cacgctggaa gaagtcgccg
     2881 acgctcagcg cgcgatggaa accggccacg gccggggcaa gatcgtcgtc cggctgcgct
     2941 aggtcgtccc gctacgctca tctggaacgg aaggcggtgg acaagccgtc gcgcgcccgc
     3001 ttccgggctg cggtgcgatt cgctagactg gcgtcggatt gaccttaagc gaggagatcc
     3061 cgatggcaaa cgtcccgcaa tggcaggccg aaggcgactg gttcgacgtc tgcaggtgcg
     3121 acattccctg tccttgcgaa ttcgcgcaac cgccgaccga caacgaatgc gaaggcatcc
     3181 tggtgtggca cgtgcgcaag ggtcgttacg gcgacgtccc gatcgacggg ctgaacgtcg
     3241 tggcgctcgg ccacttcgag ggcaatatct gggcgggtgc caaggcgacg atgggcatca
     3301 tcctcgacga gcgcggcgac ccgaagcagc gcgaggcgct gcagatgatc ttcggcggcc
     3361 aggccggcgg gtggccgggt cagttcgcgc aaacggtggc ggaagtgcgc ggcatcgaat
     3421 ttgcgcgcat cgaggtcgac atcgccggcg acctcgcgca ctggagcgca tcggttgccg
     3481 gacgggtgga ggcgaaggcc gaagcgctga ccggcccgac cacgccggcg ggcaagcgcg
     3541 tgcagacggt caatccgccc ggctcgga
//
LOCUS       DATANS010000576         1882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_784156, whole genome shotgun sequence.
ACCESSION   DATANS010000576 DATANS010000000
VERSION     DATANS010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784156"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VHT22_08480"
     CDS             <1..412
                     /locus_tag="VHT22_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HEX3137632.1"
                     /translation="EAWLQGPVILSWADVAPVAEGVNVVIHEFAHKLDMLNGDANGFP
                     PLHKGMARQAWASAFSAAYDDFCRRVEVEEDLAIDPYAAENPGEFFAVVSEAFFDTPR
                     VVLAEYPEVYAQLKAFYRQDPAARAAVQLRDAIR"
     gene            423..>1882
                     /locus_tag="VHT22_08485"
     CDS             423..>1882
                     /locus_tag="VHT22_08485"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX3137633.1"
                     /translation="MLRGFSLSNSRQALTAGFGALLILIITITLLGIWRIYAINQSIE
                     ALVQEQNLKSEMLTMLLTASQQRQHAMYRLFAAAAGAERAAVFNEYRKLNEPLFATRE
                     RFDEVDMTASERAALNQALDAATRLRDVRDQIVDLLMQNNVPLATELLLRQGLQVHDE
                     FQTSLNLVVDSKRTATLGAISQAGKAMRDALLLVAIVGSLVLVAGAWIATLVTRRIAQ
                     SESELHREKELAQVTLHSIADGVITLDAEGRVEYMNPVAEEYTGWKSDEAKGQPLDAV
                     YRVVDEQTGKPMPHPGGHEVGARGARGMTVRLLGRTGNICAIRDSSAPIHNSDDNLVG
                     WVVVFHDVSQIQEMAQQLSWQASHDALTGLVNRREFERRLGGLIEAARTDGKQHALLY
                     MDLDNFKTVNDTCGHAAGDELLLQLTTVMQSRMRGSDTLARLGGDEFGVLLESCPLEQ
                     AVRIANGLREAVRDFRFIWQEKTFGIGASAGLVTIDG"
BASE COUNT          340 a          563 c          646 g          333 t
ORIGIN      
        1 cgaagcctgg ctgcagggac cggtcattct gtcgtgggcg gatgtggcgc ccgtcgccga
       61 gggggtgaac gtcgtaatcc acgagtttgc gcacaagctg gacatgctga acggtgacgc
      121 gaacggcttt ccgcccctgc acaaggggat ggcccggcag gcgtgggcga gcgccttcag
      181 cgccgcctat gacgatttct gccggcgcgt cgaggtcgag gaagacctgg ccatcgatcc
      241 gtacgccgcc gaaaatccgg gcgagttttt tgcggtagtc agcgaggcat tttttgacac
      301 gccgcgcgtg gttctcgccg aatatccgga ggtttacgcg caactcaagg ctttttaccg
      361 ccaggatccg gcggcgcgcg cggcggtcca attgcgcgac gcaatcaggt aagcttgacg
      421 gcatgttaag ggggttttct ttatccaatt cgcggcaggc gcttaccgcg ggttttggcg
      481 cgctgctgat actgattata acgatcacgt tgctcggcat ctggcgcatc tatgcgatca
      541 atcagagcat cgaggcgctg gtccaagagc agaatctgaa gtcggagatg ctgacgatgc
      601 tgctgaccgc cagccagcag cgccagcacg cgatgtaccg gctttttgcg gcggccgctg
      661 gcgccgagcg tgccgccgtt ttcaacgaat atcgcaagct gaacgagccg ctgttcgcca
      721 cgcgcgagcg gttcgacgag gtcgacatga cggcgtcgga gcgcgccgcg ctgaatcagg
      781 cgctcgacgc tgcgacgcgc ctgcgcgacg tgcgcgacca gatcgttgac ctactgatgc
      841 agaacaacgt gccgctcgcc accgagctgt tgctgcggca gggcctacag gtgcacgacg
      901 agtttcagac gtcgctcaac ctcgtggtcg attccaagcg cacggctacg ctgggagcca
      961 tatcgcaggc cggcaaggcg atgcgcgatg cgcttctgct cgtagcgatc gtcggctcgc
     1021 tcgtgctggt tgccggcgcc tggatcgcga ccctggtaac tcgccgtatt gcgcaaagcg
     1081 aaagcgagct gcaccgcgaa aaggagctcg cgcaggttac gctgcattcg atcgcggacg
     1141 gggtcattac gctcgatgcc gaagggcggg tcgagtacat gaacccggtg gcggaggaat
     1201 ataccggctg gaagagcgac gaggccaaag gccagccgct cgacgcggtg tatcgcgtcg
     1261 tcgacgagca aaccggcaaa ccgatgccgc atccgggcgg tcacgaggtt ggtgcgcgcg
     1321 gcgcgcgtgg catgacggtg cggctgctcg ggcgcaccgg gaatatctgc gcgatccgcg
     1381 attcgtcggc gcccatccac aacagcgacg acaacctggt cggctgggtc gtggtgtttc
     1441 acgacgtgag ccagatccag gagatggcgc agcagctgtc gtggcaggcc agtcacgacg
     1501 ccttgacggg gctcgtcaat cgccgcgaat tcgagcggcg gttaggcggc ctgatcgagg
     1561 cggcgcgcac ggacggcaag cagcacgcgc tgctgtacat ggatctcgac aatttcaaga
     1621 cggtcaacga cacctgcggc cacgcggccg gcgatgaact cctgctgcag ctgactacgg
     1681 tgatgcagtc gcgcatgcgc ggcagcgaca cgctcgcgcg gctcggcggc gacgaattcg
     1741 gcgtgctgct cgaatcgtgc ccgctcgagc aggcggtgcg cattgccaac ggcctgcgcg
     1801 aagcggtgcg cgattttcgg tttatctggc aggagaagac attcggcatc ggcgccagcg
     1861 cgggcctggt gacgatcgat gg
//
LOCUS       DATANS010000577         3455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_112437, whole genome shotgun sequence.
ACCESSION   DATANS010000577 DATANS010000000
VERSION     DATANS010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112437"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VHT22_08490"
     CDS             complement(<1..295)
                     /locus_tag="VHT22_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEX3137634.1"
                     /translation="MSLLVKGQRDGRDIVRVTPQSAGWSYVGFAAHRLAQAEAISVAL
                     PGAEACLVVLSGKVTIEAGGKSWRDIGDRGSVFDDRAPYAVYLPPGETARIVAR"
     gene            complement(292..1206)
                     /gene="iolE"
                     /locus_tag="VHT22_08495"
     CDS             complement(292..1206)
                     /gene="iolE"
                     /locus_tag="VHT22_08495"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HEX3137635.1"
                     /translation="MTQAFKVKIGINPLSWSNDDLPSLGGETPLEVALAEGKAIGYQG
                     FELGNKFPRESKALAAVLAAHGLELVSGWYSGRLARRSVAEEIAAVAPHLKLLADNGA
                     TVMVYGEVADGIQGAPQPLYKRPRFVGDVAWREYADRVSAFARHTLAHGVRLAYHHHM
                     GAYVQSPSDVDRLMALTGDEVGLLFDSGHMTFAGGDPVAMLARHVRRVCHVHCKDVRP
                     QVIAMAWNRNWSFLEAVINGAFTVPGDGAVDFASLLELLQRNGYQGWLVVEAEQDPSV
                     APSYRYADLGYRNLRDLVARLMKPAEVA"
     gene            complement(1203..3056)
                     /gene="iolD"
                     /locus_tag="VHT22_08500"
     CDS             complement(1203..3056)
                     /gene="iolD"
                     /locus_tag="VHT22_08500"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HEX3137636.1"
                     /translation="MSRTIRLTMAQALVRYLAALSADTGSGIRPLFGGVFAIFGHGNV
                     AGIGEALYGHRDTLPTFRAHNEQAMAHAAIAYAKASMRRRMMACTTSIGPGATNMVTA
                     AALAHVNRLPVLLLPGDVFASRAPDPVLQQVEDFHDGGVSANDCFKPVSRYFDRIMHP
                     AQLLTALPRAIDVLTDAALCGPVTLALPQDVQTIACDYPEEFFAPTPIVFRAQPPGEA
                     DLSRAAALLRNARRPLLIAGGGVLYGLAGAALAAFVEKHGVPVAETQGGKGALRWEHP
                     MQLGAIGVTGSPAANDLARDADLVLAVGTRLTDFTTGSHSMFGGAKLVNLNVNVFDAL
                     KWRGVELVGDARLGLEALSQSLAHWQAEPSWQAKAKAVASGWRGEVERITGRRDAALP
                     YEGEVIGAVQRSARDSAAEDIVVCAAGTLPAELHKLWRVSSPGGYHVEYGYSCMGYEI
                     AGGLGVKMAKPERQVIVIVGDGSYLMLNSEIATSVMLGKKLVIVVLDNRGYGCINRLQ
                     QACGGAAFNNLFDDCVQGPDGVPRIDFAAHAASLGALSENVKTIPELEAALERARAAA
                     RSYVISIETDPDRTTEAGGWWWDVAVPEVSERETVRRARAAYEKDRKQQRP"
     gene            complement(3160..>3455)
                     /locus_tag="VHT22_08505"
     CDS             complement(3160..>3455)
                     /locus_tag="VHT22_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2090 domain-containing protein"
                     /protein_id="HEX3137637.1"
                     /translation="IEARDPYCRGVLLLGQAANADTLAAGFAEARESRTCRGFAVGRT
                     IFHEPSRKWLAGHIDDAALVRAVRGNFEALIRAWHVARGESPAAAKTMKETAA"
BASE COUNT          534 a         1214 c         1163 g          544 t
ORIGIN      
        1 tgcgcgcgac gatgcgcgcg gtttcgccgg gcggcagata gaccgcatac ggcgcgcggt
       61 cgtcgaacac gctgccgcga tcgccgatgt cgcgccaact ttttccaccc gcctcgatcg
      121 tcaccttgcc gctgaggacg acgaggcacg cttcggcgcc gggcagcgcg acgctgatcg
      181 cctccgcctg ggccagccga tgcgcggcga agccgacata gctccagccg gccgattgcg
      241 gcgtgacccg cacgatgtcg cgcccgtcgc gttgaccctt cacgagcagg ctcatgcgac
      301 ctccgcgggc ttcatcagac gcgcgaccag gtcgcgcaaa tttcgatagc cgagatcggc
      361 gtagcgataa ctgggcgcga cgctcggatc ctgctcggct tcgacgacca gccagccctg
      421 gtacccgttt cgttgcaaca actcgagcag cgacgcgaag tcgaccgcgc cgtcgccggg
      481 cacggtaaag gcgccgttga tcaccgcctc gagaaagctc cagttgcgat tccacgccat
      541 tgcaatcact tgcggccgca cgtccttgca gtgcacatgg cagacgcgac ggacgtggcg
      601 cgcgagcatc gcgaccggat cgcctccggc gaaagtcatg tggccgctgt cgaacagcag
      661 gcccacctcg tcaccggtca gcgccatcag gcggtcgacg tcggatggcg actggacgta
      721 cgcgcccatg tggtggtgat aggcgagccg cacgccgtgc gcgagcgtgt gccgcgcgaa
      781 cgcactgacg cggtcggcgt actcgcgcca cgcgacgtcc ccgacgaagc gcgggcgctt
      841 gtacagcggc tgcggcgcgc cctggatacc gtcggcgact tcgccgtaga ccatcactgt
      901 cgcgccgttg tccgccagga gcttgagatg cggcgcgact gcggcgatct cctcggccac
      961 ggagcggcgc gcgagccggc ccgagtacca gccggacacg agctcgagtc cgtgcgcggc
     1021 cagcaccgcg gcgagtgcct tcgattcgcg cggaaacttg ttgcccagct cgaagccctg
     1081 gtatccgatc gccttgcctt cggcgagcgc gacctcgagc ggcgtctcgc cgccgagcga
     1141 ggggagatcg tcgttgctcc acgagagcgg gttgatgccg atcttcacct tgaaggcttg
     1201 agtcatggcc gctgctgctt cctgtccttt tcgtacgctg cgcgcgcgcg gcgcacggtc
     1261 tcgcgctcgg acacctcggg cacggcgacg tcccaccacc agccaccggc ctcggtggtg
     1321 cggtcgggat ccgtctcgat ggagatcacg tagcttcgcg ccgcggcgcg cgctcgctcg
     1381 agcgccgctt cgagctcagg aatcgtcttg acgttctcgg agagagcacc gagcgaggcc
     1441 gcgtgcgcag cgaaatcgat gcgcggcacg ccgtccggcc cttgcacgca atcgtcgaaa
     1501 aggttgttga acgccgcccc gccgcaggcc tgctgcagcc ggttgatgca gccatagccg
     1561 cgattgtcga gcacgacgat gacgagcttt ttgcccagca tcaccgaggt ggcgatctcg
     1621 ctgttgagca tcaggtagct gccatcgccg acgatcacga tcacctgccg ctcgggcttg
     1681 gccatcttga cgccgagtcc tcccgcgatc tcgtagccca tgcacgagta gccgtattcg
     1741 acgtggtagc cgcccggcga cgacacgcgc cagagtttgt gcagctcggc aggcaaggtg
     1801 ccggcggcgc agacgacaat atcctcggcc gcggagtcac gcgccgagcg ctgcaccgcg
     1861 ccgatcactt cgccttcgta cggcagcgcg gcgtcgcgac ggccggtaat gcgctcgacc
     1921 tcgcccctcc agccgctcgc caccgctttg gccttggctt gccacgaagg ctcggcttgc
     1981 cagtgcgcaa gcgactgcga caacgcctcg aggcccaacc gggcgtcgcc gaccagctcg
     2041 acgccgcgcc atttgagcgc gtcgaagacg ttgacgttga ggttcaccag cttcgcgccg
     2101 ccgaacatcg aatgcgagcc ggtcgtgaaa tccgttaggc gcgttccgac cgcgagcacc
     2161 aggtcggcgt cgcgcgcgag atcattggcg gcgggcgagc cggttacgcc gatcgcgccg
     2221 agctgcatcg gatgttccca gcggagcgca cccttgccgc cttgcgtctc ggcgaccggg
     2281 acgccgtgct tttcgacgaa cgcggcgagc gcggcgccgg ccagcccgta caggacgccg
     2341 ccgccggcga tcagcagcgg gcgccgcgcg ttgcgcaaca gcgcggcggc gcgcgaaaga
     2401 tcggcttcac ccggcggctg cgcgcgaaag actatcgggg tgggggcgaa gaattcctcc
     2461 ggataatcgc aggcgatcgt ctgcacgtcc tgcggcagcg ccagcgtcac cggaccgcag
     2521 agcgcggcgt cggtcagtac gtcgattgcg cgcggcagcg cggtcagcaa ttgcgctgga
     2581 tgcatgatgc ggtcgaaata gcgcgacacc ggcttgaagc aatcgttggc cgatacgcca
     2641 ccgtcgtgga agtcttcgac ctgttgcagc accgggtccg gcgcgcgcga ggcgaacaca
     2701 tcgccgggca gcagcagcac cggcaggcgg ttgacatgcg ccagggcggc ggcggtcacc
     2761 atgttggtcg cacccgggcc gatcgacgtc gtgcacgcca tcatccgtcg gcgcatgctg
     2821 gccttggcat aggcgatcgc cgcatgcgcc atcgcctgct cgttgtgggc gcggaacgtc
     2881 ggcaaggtgt cgcgatgacc atagagcgcc tcgccgatgc cagctacgtt gccgtggccg
     2941 aagatcgcga acacgccgcc gaacaacggg cggatgccgg acccggtgtc ggccgacagc
     3001 gccgcaagat agcgtaccag cgcctgcgcc atggtgagcc ggatcgtgcg gctcatgctg
     3061 cggcctcttc gatcgtgtcc gcggtcacgc aatggcgtgt cggccgcctg cggccttcgc
     3121 cggtcgcacc cccgctgcgc gcggcccctg ctccgcggct catgccgctg tctctttcat
     3181 cgttttcgcc gcagcggggc tctccccgcg cgcgacgtgc caggcgcgta tcagcgcctc
     3241 gaaattcccg cgcacggcgc gcaccagcgc cgcatcgtcg atatggccgg ccagccactt
     3301 gcggctgggc tcgtggaaga tcgtgcggcc gacggcgaag ccgcggcaag tccggctctc
     3361 gcgggcttcg gcgaatccgg cggcgagcgt gtcggcgttc gcagcctgcc cgagcagcag
     3421 caccccgcgg caatacgggt cgcgcgcttc gatca
//
LOCUS       DATANS010000578         4019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_532593, whole genome shotgun sequence.
ACCESSION   DATANS010000578 DATANS010000000
VERSION     DATANS010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532593"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..1290
                     /gene="gshB"
                     /locus_tag="VHT22_08510"
     CDS             343..1290
                     /gene="gshB"
                     /locus_tag="VHT22_08510"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEX3137638.1"
                     /translation="MEFAFIIDPPEGLKAYKDTSVSMMRALQARGHSIYLTEPKELFW
                     RAAATQARVRRAEVTADDHDWYRAAEPMIRPLASFAAVLMRKDPPFDMEYVYSTYLLE
                     AAEHEGAYVINGPRAIRDHNEKWAIAEFPQFTVPTLVTRDPDLVASFIDEHADVILKP
                     LDGMGGSSIFRVRRDDVNRNVIVETMAHFGARSVMAQRFIPEIVDGDKRIILINGEAA
                     PHALARIPRAGETRGNLAAGGRGVARPLTTRDREIADTIGRALAPKGLFVIGLDVIGD
                     YLTEVNVTSPTGFVEITQQTGFDVAGAFAQALEKSVGAR"
     gene            1433..1846
                     /locus_tag="VHT22_08515"
     CDS             1433..1846
                     /locus_tag="VHT22_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEX3137639.1"
                     /translation="MIGILIVAHGTLSDSLIGAVTHVLGSRPPQFDGMRVAAADDPLA
                     LLPRAREVVKSLDSGDGVLIFSDIFGASPCNLAIKLLQPGRIEAIAGVNLPMLVRAFT
                     YRNKGMDTMIKKAISGGCDGVVRVEVDPIYAATRN"
     gene            1827..2096
                     /locus_tag="VHT22_08520"
     CDS             1827..2096
                     /locus_tag="VHT22_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX3137640.1"
                     /translation="MQQREIEIVNKLGLHARASAKLTQLAAKYQCDVSLARNGRKINA
                     KSIMGVMMLAAGKGSRVVLETDGPDETEALDAITALIGDYFGEGQ"
     gene            2259..2528
                     /locus_tag="VHT22_08525"
     CDS             2259..2528
                     /locus_tag="VHT22_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX3137641.1"
                     /translation="MQTLDIGITNRLGLHARAAAQIVRLASQFRCSVSLIVNGRRASA
                     RSMVAVMMLAASMGSTVRLETNGPDEAAAMVAMVQLIGDGFGETR"
     gene            2545..>4019
                     /gene="ptsP"
                     /locus_tag="VHT22_08530"
     CDS             2545..>4019
                     /gene="ptsP"
                     /locus_tag="VHT22_08530"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX3137642.1"
                     /translation="MSFVLHGIGVSGGVAIGRAQLVSHATLEVDHYAIRPEQVPAEID
                     RLTLALRAVQKELEALHGAMTSGDAPAEFGAFLDVHWMILTDPTLSEAPKRMIADQLC
                     NAEWALTQQMNVLVEQFESIEDPYLRERKADVVQVVERVLKVLLGKAQRRPASAEDET
                     ILVAHDLSPADVIQFKSHHFAAFLTDLGGASSHTAIVARSLNVPAVVATHNARGLIRE
                     HELLIVDGAKNVVIVNPDRSVLAEYRLKQGEIELARKKLKRLKTTPAATIDGADIELF
                     ANIELPDDLDQALDNGAGGIGLFRSEFLFLNRQGLPSEDEQFEAYRQVAAGMRGKPVV
                     IRTFDLGADKSKEGLDGLVRVAPNPALGLRAVRFCLAEPRLFLTQLRAILRASHYGNI
                     RILLPMLASVAEIDQTLVLLAQAKGSLLERGVPFNPSIEVGGMIEIPAAVLTIEPFAR
                     RLDFLSIGTNDLIQYTLAVDRTDDRVSRMYEPLHPAILRVLR"
BASE COUNT          743 a         1305 c         1302 g          669 t
ORIGIN      
        1 caggcttagg cccacgatgt cgcccaggcg cggatcaagc gatgcaacca ggcgccaagc
       61 ctcggccatc gcgcccgaaa ggctgctcat ggaacgcacc tcgatgcccg gtcgcgatcc
      121 ggcgaaccgg ccaacgcgtc ataggcacag tattgcctat acacagggat cctcattact
      181 ctaccaataa ttcaggatga tacgaggtcc caggccgccg ccaaacaggc aaatcgatgc
      241 ctaattacaa gcgaggcccc gctcggattc ggccagcctc cgtggtgtcg gggctttgcc
      301 gatttacggt acgatgggcg cccgccaatc gccctgcgct gcatggaatt cgcattcatc
      361 atcgatccgc ccgaagggct gaaggcgtac aaggacacga gcgtctcgat gatgcgtgcg
      421 ctgcaggcac gcggacactc gatctacctg accgagccca aggagctttt ctggcgcgcg
      481 gcggcgacgc aggctcgcgt gcgccgcgcc gaggtcacgg ccgatgacca cgactggtat
      541 cgcgctgccg agccgatgat ccgaccgctg gcctcgttcg ccgcggtgct gatgcgcaag
      601 gatccaccgt tcgacatgga atacgtctat tcgacctacc tgctcgaggc ggccgagcac
      661 gaaggcgcgt acgtcatcaa cgggcctcgc gcgatacgcg accacaacga aaaatgggcg
      721 atcgccgagt ttccccagtt caccgttccg acgctggtga cgcgcgatcc cgacctcgtc
      781 gccagcttca tcgatgagca cgccgacgtt atcctgaagc cgctcgacgg catgggcgga
      841 tcgtcgatct ttcgcgtgcg ccgcgacgac gtcaatcgca atgtgatcgt cgagacgatg
      901 gcgcatttcg gcgcgcgcag cgtcatggcg cagcgattca ttcccgaaat cgtcgacggg
      961 gacaagcgca ttatcctgat caatggcgag gcggcgccgc acgcgctcgc acgcattccc
     1021 agggccgggg agacgcgcgg caacctcgcg gccggtggcc gcggcgtcgc gcgtccgctg
     1081 acgacgcgcg atcgcgagat cgccgacacg atagggcgtg cgctcgcgcc caagggcctg
     1141 ttcgtcatcg ggctcgacgt gatcggcgac tacctgaccg aggtcaacgt gaccagtccg
     1201 accgggtttg tggagatcac ccagcagacc ggattcgacg tcgccggcgc ctttgcgcag
     1261 gcgctggaaa agagcgtcgg cgcgcgttga gacgcggccc ggcgatagcc ttgccgcgca
     1321 gccgcgcgtg ctaagataaa gccacccgcg cgggcgagga atcgaaggtc gggaccaaat
     1381 gacaaacgcc gcggggcata cacatggcgc gggccgtcag gggacagaca gcatgatcgg
     1441 gatcctcatc gtcgcccacg gcaccttgag cgacagcctg atcggcgcgg tgacccacgt
     1501 gctcggaagc cgcccgcccc agttcgacgg catgcgcgtc gccgcggctg acgatccgct
     1561 ggcgctgctg ccgcgggcgc gcgaggtcgt caaatcgctc gacagcggcg acggcgtgct
     1621 gatcttctcc gacatcttcg gcgcttcgcc ctgcaacctt gccatcaagc tgctgcaacc
     1681 agggcgcatc gaagccatcg ccggcgtcaa cctgccgatg ctcgtgcgcg ccttcaccta
     1741 tcgcaacaag ggcatggaca cgatgatcaa gaaggccatc agcggcggct gtgacggcgt
     1801 cgtgcgcgtc gaggtggacc cgatctatgc agcaacgcga aattgaaatc gtcaacaagc
     1861 tgggattgca tgcgcgcgcg tcggcgaagc tgacgcagct ggctgcgaaa tatcaatgcg
     1921 atgtgtcgct ggcgcgcaat ggacgcaaga tcaatgccaa gagcatcatg ggcgtgatga
     1981 tgctggctgc tggcaagggc tcccgggtcg tgctcgagac cgacgggccc gacgagaccg
     2041 aggcgctcga cgcgatcacc gcgctgatcg gcgattattt cggcgaagga cagtgacgac
     2101 cgtatcgctc gcgcaacatg gcgtcgccga tggccgacac atcgagttgc atcattcgtg
     2161 gaaccaaagc ccgcgacgca ggctcaatga acaggcgtgc ttcccggtcg taaccaggcg
     2221 cgcaggctct tacctcaact gcgacgggcg tctcgaacat gcaaactctc gacatcggca
     2281 tcaccaatcg cttgggtctt catgcgcgcg cggcggcaca gattgtgcgt ctcgcatcgc
     2341 aattccggtg cagcgtctcg ctgatcgtga acggacggcg tgcgagcgcg cgcagcatgg
     2401 tcgccgtgat gatgctggca gccagcatgg gcagcacggt caggctggaa accaatggcc
     2461 cggacgaggc agcggcgatg gtggcgatgg tccagctgat cggcgacggg ttcggcgaga
     2521 cgcgttaacc cggcgtcctc gccggtgagc tttgtcctgc acggcatcgg tgtttcaggc
     2581 ggcgtcgcca tcggccgggc gcagctggtc tcgcatgcga cgctggaagt cgaccactat
     2641 gcgatccgcc ccgagcaggt ccccgccgag atcgaccggc tgacgctggc gctccgggcg
     2701 gtccagaagg agctcgaggc gctgcacggc gcaatgacct cgggcgatgc accggccgag
     2761 ttcggtgcat ttctggacgt gcactggatg atcctcaccg atccgacgct ttccgaggca
     2821 ccgaagcgga tgatcgccga tcagctctgc aacgccgaat gggcactgac gcagcagatg
     2881 aatgtgctgg tcgagcagtt cgagtcgatc gaggaccctt acctgcgcga gcgcaaggcc
     2941 gatgtcgtgc aggtggtcga gcgcgtgctc aaagtcctgc ttggcaaagc gcagcggcgt
     3001 cccgccagcg ccgaagacga gacgatcctg gtcgcccacg atctttcgcc tgcagacgtc
     3061 attcagttca agagccacca tttcgccgcg ttcctgaccg atctgggcgg cgccagctcc
     3121 cataccgcga tcgtcgcgcg cagtctcaac gtccccgccg tcgtggcgac gcacaatgcg
     3181 cgtgggctga tccgcgagca cgagctgttg atcgtcgacg gcgccaagaa cgtcgtcatc
     3241 gtcaaccccg atcgttcggt gctcgccgag taccggctga agcagggcga gatcgagctg
     3301 gcacggaaga agcttaagcg gctcaagacg acgcccgcgg cgacgatcga cggcgccgac
     3361 atcgagctgt tcgccaacat cgagctcccc gacgatctcg atcaagcgct ggacaacggc
     3421 gccgggggca tcggactttt ccgcagcgag ttcctgtttc tcaatcgtca gggactgccg
     3481 tcggaagacg agcagttcga agcataccgg caggtcgctg ccggcatgcg cggcaagccg
     3541 gtagtgattc gcacgttcga tctgggcgcc gacaagagca aggaaggcct cgacgggctg
     3601 gtgcgcgtcg cgccgaatcc tgcgctggga ttgcgcgccg tgcgcttttg cctcgccgag
     3661 ccgcggctgt tcctgacgca gctccgggcg atcctgcgcg cctcgcatta cggcaacatc
     3721 cggatcctgc tgccgatgct cgcctcggtc gccgaaatcg atcagacgct ggtgctgctc
     3781 gcccaggcca agggcagcct gctcgaacgg ggcgtgccat tcaatccgtc gatcgaggtc
     3841 ggcggcatga tcgaaattcc ggcggcggtg ctgacgatcg agccgttcgc gcgccggctg
     3901 gatttcctgt cgataggcac caacgatctg atccagtaca cgctggccgt cgatcgcacc
     3961 gacgatcggg tgtcgcgcat gtacgagccg ctgcatccgg cgatcctgcg cgtgctgcg
//
LOCUS       DATANS010000579         1954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_237009, whole genome shotgun sequence.
ACCESSION   DATANS010000579 DATANS010000000
VERSION     DATANS010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237009"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VHT22_08535"
     CDS             <1..734
                     /locus_tag="VHT22_08535"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX3137643.1"
                     /translation="RLGIIDEIAADAGTAVKDADLVLLAVPLGQTRAVLAQIAPHLAA
                     HTVVSDAGSTKRDVIAAARRQLGATFARFVPAHPIAGTEKSGAEAAFAELFENRNLVV
                     TPEPETSARALKLVTAAWKLAGMRILSMDAAKHDRMFALVSHLPHLLSFTAVGNIAGY
                     PDAARLFQHIGGGFRDLTRIAGSSPEMWRDICIANRDPILAALDGYLSDLEAVRGMVE
                     ASDGQGLAMKFSTARNARAKWIKPQ"
     gene            776..>1954
                     /gene="aroA"
                     /locus_tag="VHT22_08540"
     CDS             776..>1954
                     /gene="aroA"
                     /locus_tag="VHT22_08540"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX3137644.1"
                     /translation="MEYLDLAPVRRASGTVKLPGSKSISNRILLLAAIAEGETRIHDV
                     LDSDDTRYMLNALRKLGVEWTQDELQALRVTGAQGVFPNKHAELFLGNAGTAVRPLTA
                     VLALCGGEYKVDGVARMRERPIGDLVDALKSFGAAITYLGAAGFPPLAIHPGKIRAGT
                     DVKIRGDVSSQFLTALLMSLPLLRARTRVKVEGELVSKPYVDITLNMMRRFGVEVARD
                     EWRTFTVPGAVRYRSPGNIYVEGDASTASYFLAAGVISGLTGGGPVRVEGVGQASIQG
                     DVRFTETLREMGASVEIGANWISAAAGPAAMRTRKLRAVNADFNHIPDAAMTVAMLAL
                     FADGPSTLRNIGSWRVKETDRIVAMATELRKVGAQVEAGPDSLKITPPAALLPATIDT
                     Y"
BASE COUNT          345 a          618 c          641 g          350 t
ORIGIN      
        1 ggcgtttggg catcatcgat gaaatcgccg ccgacgcggg aactgcggtc aaagacgcgg
       61 atctcgtctt gctcgccgtg ccgctcggac agacgcgcgc ggtgcttgcg cagatcgcgc
      121 cgcatcttgc cgcgcacacc gtggtcagcg atgccggcag caccaagcgc gacgtcatcg
      181 cagccgcgcg gcgccagctc ggcgcgacgt ttgcgcggtt cgtgcccgcg cacccgattg
      241 ccggcaccga aaaaagcggc gctgaggcgg ccttcgcgga actgttcgaa aatcgtaatc
      301 tggtcgtgac accggaaccg gaaacctcgg cgcgcgcgct gaaactcgtg acggccgcat
      361 ggaagctcgc gggcatgcgc atcctttcga tggacgcggc caaacacgac cggatgttcg
      421 cgctcgtgag tcatttgccg catctgctgt cgttcactgc cgtcggtaac atcgcgggct
      481 acccggatgc cgcccgatta ttccaacaca tcggcggcgg atttcgcgat ctcacgcgta
      541 tcgccggcag ctctccggag atgtggcgcg atatctgtat cgccaaccgc gatcccatcc
      601 tggctgcgct cgacggttat ctgtccgatc tcgaagccgt gcgcggcatg gtcgaagcga
      661 gcgacgggca ggggctcgcc atgaaattct ccaccgcgcg caacgcgcgc gcgaagtgga
      721 tcaagccgca gtgagccgcg tgcttattct tttgccggac taataaacga cgcgcatgga
      781 atatctcgat cttgcccccg tgcggcgcgc cagcggcacc gtcaagctgc cggggtctaa
      841 aagcatctcc aatcgcatcc tgctgctcgc ggcaatcgcg gagggcgaaa cgcgcattca
      901 tgacgtgctc gattcggacg acacgcgcta catgctcaat gcgctgcgca agctcggcgt
      961 cgagtggaca caggacgaat tgcaggcatt gcgcgtgacc ggcgcgcagg gcgtcttccc
     1021 gaacaagcat gccgagttgt tcctcggcaa tgccggtact gccgtgcgtc cgctgaccgc
     1081 ggtgctcgcg ctgtgcggcg gcgaatataa ggtcgacggg gttgcgcgca tgcgcgagcg
     1141 cccaatcggc gacctggtcg atgcgctcaa gtcgttcggc gccgccataa cttatctggg
     1201 tgccgccggc ttcccgccgc tcgccataca tccgggcaag atccgcgctg gaacggacgt
     1261 aaaaatccgc ggcgatgtgt cgagccagtt tctgactgcg ctgctgatga gtctgccgct
     1321 gttgcgcgcg cgcacgcggg tcaaagttga aggcgagctg gtgtccaagc catacgtcga
     1381 catcacgctc aacatgatgc ggcgcttcgg cgtcgaggtg gcgcgcgacg agtggcgcac
     1441 tttcactgtg ccgggggctg tgcgctaccg cagtcccggc aatatttacg tcgaaggtga
     1501 tgcgtcgacc gcctcgtact ttctcgcggc gggcgtgatc agcggtttga cgggcggcgg
     1561 gccggtgcgg gtcgaaggcg ttggacaagc gagcattcag ggcgacgtgc gcttcaccga
     1621 aacgctgcgc gaaatgggcg ccagcgtcga gatcggcgcg aactggattt cggccgccgc
     1681 cggtcccgct gcgatgcgca cgcgcaagct gcgcgcggta aatgcggact tcaatcacat
     1741 tcccgatgcg gcgatgacgg tcgcgatgct cgctttattt gccgatggcc cgagcacgct
     1801 gcgcaatatc ggcagctggc gcgtgaagga aaccgaccgc attgtggcga tggcgaccga
     1861 actcaggaaa gtaggcgcgc aggtcgaagc gggtcccgat agcctgaaga tcacgccgcc
     1921 cgcggcgttg ttgccggcca cgattgacac ctac
//
LOCUS       DATANS010000580         1766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_281840, whole genome shotgun sequence.
ACCESSION   DATANS010000580 DATANS010000000
VERSION     DATANS010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281840"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="VHT22_08545"
     CDS             complement(<1..1055)
                     /locus_tag="VHT22_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX3137645.1"
                     /translation="MSQPASLTWLAGHEFRLGWRDWTSLMTAGGRTRARTATIALIAF
                     AAFMHVFGFWTVASYANADLESETSALVGITGSLLLSWSLLLSQAMESVTRAFYSRSD
                     LDLILTSPASARKVFAVRMGRIAGAAVAIAVLLAAPFINVLAFLGGARWLAAYGVVAA
                     MGAVATAAALALTIALFRVIGAKRTRLVAQIVAAVIGAAFVIGLQMAAIFSAGSLSRF
                     AALQSDWMLSHAPDTHSAFWWPARAVLGDYGALAGVMMFAVVVLAASIALFSRRFGEH
                     AVAAAGVSSAIVRHRRWSLRFRRRSPARVLRHKEWTLLLRDPWLISQTLMQILYLLPP
                     AMLLWVTFHDHNDSFVLL"
     gene            complement(1052..>1766)
                     /locus_tag="VHT22_08550"
     CDS             complement(1052..>1766)
                     /locus_tag="VHT22_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3137646.1"
                     /translation="SKRFDRPAVDALDLTVYGGEFYVLVGPNGAGKTTTLRMVVGLLR
                     PDAGSICIEGVDALADPVAAKQVTAWLSDEPMIYDKLTPYEYLEFVAGLWRIEPATAE
                     ARARDLIGWLGLEPHAHERCEGFSKGMRQKVALAGALVHDPKLIILDEPLTGLDAGTA
                     RQVKNVLRERVRAGGTVIMTTHILEVAERMADRIGVIAGGRLIAEGTLEDLRRQTGKG
                     ATSLEDTFLALVAEEVVAA"
BASE COUNT          373 a          571 c          563 g          259 t
ORIGIN      
        1 aggagtacga acgagtcgtt gtgatcgtgg aatgtcaccc acagaagcat cgcgggtgga
       61 agcagataaa ggatctgcat cagggtctgc gaaatcagcc acggatcgcg cagcagcaac
      121 gtccattcct tgtgccgcag cacgcgggca ggcgagcggc ggcgaaaacg gagcgaccag
      181 cggcgatggc gcacgatcgc gctcgagaca cctgccgctg ccacggcatg ctcgccaaat
      241 ctgcgcgaga acagcgcgat cgacgccgca agcacgacga cagcgaacat catgactccc
      301 gccagcgcac cgtaatctcc gagcacggca cgggcaggcc accagaacgc gctgtgcgta
      361 tcgggcgcat gcgacagcat ccagtccgat tgcagggcgg caaagcgtga cagactgccg
      421 gccgaaaata tcgcggccat ctgcaggccg atgacgaatg cagcgccgat gaccgccgcc
      481 acgatctgcg caaccaggcg cgtgcgtttg gcgccgatta cgcggaacag ggcgatggtg
      541 agcgccagag ctgctgccgt ggccaccgca cccatggcgg cgacgacacc gtaggcggcc
      601 agccagcggg ccccgccgag aaaggccagc acgttgatga aaggcgctgc gagcaggaca
      661 gcgatggcga cggccgcgcc cgcgatgcga cccatgcgca cggcgaatac ttttcgcgcg
      721 gatgcaggcg atgtgagaat gagatcaaga tcggagcgcg aatagaacgc gcgcgtcacg
      781 gactccatcg cctgcgacaa aagcagtgac cacgacagca acagcgagcc cgttataccg
      841 accagcgccg aagtctctga ttcaagatcg gcattcgcgt agctcgccac cgtccagaac
      901 ccgaagacgt gcatgaaggc tgcaaaggcg atcagcgcga tcgtggccgt acgggcccgc
      961 gtgcgcccgc ccgccgtcat cagggaggtc cagtcgcgcc agccgaggcg aaattcgtgg
     1021 cctgcgagcc aggtgagcga agcgggctgg ctcacgcggc aaccacttct tcggcgacga
     1081 gcgcgagaaa tgtatcttcc aggcttgtcg cgccctttcc ggtctgacgg cgcagatcct
     1141 cgagcgtgcc ttcggcgatg aggcgtccgc cggcgatgac gccaatgcgg tcagccatgc
     1201 gctccgccac ttcaagaatg tgcgtcgtca tgatgacggt gccgcccgcg cgtacgcgct
     1261 cgcgcaacac gttcttcacc tggcgcgccg tgccggcatc aagtccggtc aacggttcgt
     1321 cgagaatgat gagcttcgga tcgtgaacga gcgcgccggc cagcgccact ttctggcgca
     1381 tgcctttgga aaatccttcg cagcgctcgt gcgcatgcgg ttcgagacca agccacccga
     1441 tcagatcccg tgcgcgggct tcggcagttg caggctcgat gcgccacagg cccgcgacaa
     1501 actcaagata ctcgtaaggc gtcagcttgt cgtagatcat cggctcgtcc gaaagccagg
     1561 cagtgacctg cttggcagca accggatcgg caagtgcatc gacgccttcg atacagatcg
     1621 agccggcatc cggccgcagc aatcccacga ccatccgcaa ggtagttgtc ttgccggcgc
     1681 cgttgggacc gacgagcaca tagaactcgc cgccatagac cgtgagatcg agcgcatcga
     1741 ccgcggggcg atcgaaccgc ttgctg
//
LOCUS       DATANS010000581         1681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_365736, whole genome shotgun sequence.
ACCESSION   DATANS010000581 DATANS010000000
VERSION     DATANS010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365736"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VHT22_08555"
     CDS             <1..333
                     /locus_tag="VHT22_08555"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3137647.1"
                     /translation="LMLVLPDVAAYLVLPLLGIALNGTSSVLYATIGDVIDPDRLPRA
                     FGLFYTINSVCGLIVPLVFGYVGDAFGVQLSIAICGVLAFATLPLCFLLARALHPGTH
                     AAAQARAR"
     gene            406..1059
                     /locus_tag="VHT22_08560"
     CDS             406..1059
                     /locus_tag="VHT22_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase"
                     /protein_id="HEX3137648.1"
                     /translation="MAKQRISYVPLEKMDAAQRAEMERCAREGTPRPESTAVRAHVPA
                     CFWFFANSWNDIFRNGILDHALKELCRVYVSRSVICEFCGNQRSIKGANQGLQETHYD
                     DLINFEKSVKYDERQKAALAYAEAITWRLDTDDAFWERMYKHFNEPELVELGCAVGLT
                     LGQQSWLRLLNIEHHQVMPGTGASMAPGFETAEALAKSKASPDYWAKKPAAPKKNVA"
     gene            1063..>1681
                     /locus_tag="VHT22_08565"
     CDS             1063..>1681
                     /locus_tag="VHT22_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3137649.1"
                     /translation="MSITGVTADLPAGQIFYRDSGGEGAPVVFLHAASGNSALWEHQI
                     TAVIGAGFRFIALDHRAAARGSNSTALLEGLLAKLNVGRCHLLGTAAGGGAALQFALA
                     YPAKVRGLVVANSIGFVLDREYLDMSNRIRPAPKFNELPLDFRELGPSYRAANAEGAA
                     RWLDLSRQGQRSTGARTENGGNVEVTWAKIETLKMPTLLVTGDADL"
BASE COUNT          309 a          545 c          523 g          304 t
ORIGIN      
        1 ctgatgctcg tgctgcccga cgtcgctgcg tacctcgtgc tgccgctgct cggcattgcg
       61 ctgaacggca cgtcgtcggt gctgtatgcg accatcggcg acgtcatcga ccccgaccgc
      121 ctgccgcgcg cgtttggcct cttctatacg atcaactctg tgtgcggctt gatcgtcccg
      181 ctcgtcttcg gctacgtagg cgacgcgttc ggcgttcaat tgtcgatcgc gatctgcggc
      241 gtcctcgcct ttgccacgct gccattgtgc ttcctgctcg cgcgcgccct gcacccgggc
      301 acacacgccg ccgcccaggc ccgcgcgcgg taaacacgct attcggcagc ttgccggagc
      361 gttccgctaa aataacggct ggcacgacca tctgaggaga tacccatggc caaacaacgc
      421 atcagctacg ttccgctcga aaaaatggat gccgcgcaac gcgctgaaat ggagcgctgc
      481 gcgcgcgaag gcacgccgcg cccggaaagc accgccgtgc gcgcgcacgt gcccgcgtgc
      541 ttctggtttt ttgcgaattc gtggaacgat attttccgca acggcatact cgaccacgcg
      601 ctaaaggagc tgtgccgcgt ctacgtgtcg cgatcggtca tctgcgaatt ctgcggcaac
      661 cagcgctcga tcaagggcgc caatcagggc ctgcaggaaa cgcattacga cgacctgatc
      721 aatttcgaga agtcggtcaa gtacgacgag cgccagaaag cggcactcgc ctatgccgaa
      781 gcaatcacgt ggcggctcga caccgacgac gcgttctggg agcgcatgta caagcacttc
      841 aacgagccgg agctcgtcga gctcggctgc gcggttggtc tcacgctggg acagcaaagc
      901 tggctgcggc tcttgaatat cgagcatcat caagtgatgc ctggcacggg cgcgtcgatg
      961 gcccccggct tcgaaacggc agaagcgctc gccaaatcca aagcgtcgcc cgactactgg
     1021 gccaaaaaac ccgccgcgcc gaaaaagaac gtcgcgtagc gggtgtcgat caccggggtc
     1081 acggcggacc tgcccgccgg ccagattttc tatcgcgact cgggcggcga aggcgcgccc
     1141 gtcgtctttt tgcacgccgc ttcgggcaac agcgcgttgt gggagcatca gatcacggca
     1201 gtcatcggcg cgggattccg ttttatcgcg ctcgaccatc gcgcggccgc gcgcggcagc
     1261 aattcaacgg cgctgctcga aggcctgctt gcaaaactga atgtcgggcg ctgtcatttg
     1321 ctcggcacgg cggcgggcgg cggtgcggcc ctgcaattcg cgctcgcgta tccggccaaa
     1381 gttagaggcc tcgtcgtcgc gaacagcatc ggctttgtgc tcgaccgcga atatttggat
     1441 atgagcaacc gcatccggcc cgcgccgaag ttcaatgagc tgccgctcga ctttcgcgag
     1501 ttggggcctt cttatcgcgc ggcgaatgcc gaaggcgcgg cacgctggct cgacctgtcg
     1561 cgacagggcc agcgcagcac gggggcccgc accgaaaacg gcggcaacgt ggaagtgacg
     1621 tgggcgaaaa tcgagacgct gaaaatgccg acgctgctgg tgaccggtga cgccgatctc
     1681 t
//
LOCUS       DATANS010000582         1650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_702942, whole genome shotgun sequence.
ACCESSION   DATANS010000582 DATANS010000000
VERSION     DATANS010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702942"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="VHT22_08570"
     CDS             complement(<1..1013)
                     /locus_tag="VHT22_08570"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX3137650.1"
                     /translation="MRLAAGTLATWLIGAPRWLVGLLIIAVFLLHPLTQFGKTTLLAK
                     LEAISYDTRLKLTLPNRGDPQVVIIDINEPSLQREGRWPWSRDKLARLTTQLLDRYQA
                     RAVGFDVVFAEPDRSSGIDVLERLAQRDLREDRGYVATLDRLRPELDFDARFADAIRG
                     RPVILGFGFTYEAQQIGALPPPLFRAQDIGGGIIPIQAAVGYTAAIGELAQAAAGSGH
                     VDPFFDADNVVRRVPMVELYRNAYYPALSLAIARVVLEAKQVTPHFDSDQRLDGFDIG
                     GLALPTAADGTALIPFRGKPKTFRYYSAADVLAGSAPADFAGAIALVGTTAKGLQDLR
                     PTPL"
     gene            complement(1025..>1650)
                     /locus_tag="VHT22_08575"
     CDS             complement(1025..>1650)
                     /locus_tag="VHT22_08575"
                     /inference="COORDINATES: protein motif:HMM:NF037752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HEX3137651.1"
                     /translation="SGLISGTPEAAGASTLTVTANNGCNAPAVQTFTLTVDRATTTLS
                     LAALPQIAVFGQTVTVALVASGGPPSPQGPVQLCVRGTGMFCGPPFDTVPPATPPEKI
                     AAPLSGVLDPNGRADFRLTGLTIDAFTLSAFYAGDSSHLPAGAGPVDELVIKGVLLPS
                     RPDGKANVAASTSDPSGIPVLSSIGLALLSVAVAALAMATLRRRTRR"
BASE COUNT          291 a          556 c          566 g          237 t
ORIGIN      
        1 agcggagtcg ggcgcagatc ctgcagtccc ttggccgtcg ttccgaccag cgcgatcgca
       61 cctgcgaaat ccgccggcgc gctgcccgcc agcacgtcgg cggccgaata gtagcgaaaa
      121 gtcttgggct tgccgcgaaa cgggatcagc gccgttccgt ccgctgcagt gggaagggcc
      181 aaaccgccga tatcgaaacc gtcgagcctt tggtcggaat cgaaatgcgg cgtcacctgc
      241 ttcgcttcga gcacgacccg cgcgattgcc agcgacagcg ccgggtagta ggcattgcga
      301 taaagctcga ccatcggaac gcggcgcacg acattgtcag catcgaaaaa cgggtcgaca
      361 tggccgctgc ccgcggccgc ctgcgcgagc tccccgatgg ccgccgtata gccgacggcc
      421 gcctggatcg gaatgatgcc gccgccgata tcctgggcgc ggaacaacgg cggcggaaga
      481 gcgccgatct gctgcgcctc gtaggtgaag ccgaacccca gaatcacagg cctgccgcga
      541 atggcgtcgg cgaaccgcgc gtcgaagtcg agctcggggc gcagcctgtc gagcgtcgcg
      601 acatagccgc ggtcttccct gaggtcgcgc tgcgccagcc gctcgaggac gtcgatgccc
      661 gacgatcggt ccggttcggc gaacacgacg tcgaacccga cggctcgcgc ctgatagcga
      721 tccagcaatt gggtcgtcaa ccgggccagc ttgtcgcgag accagggcca gcgtccttcg
      781 cgctgcaggc tgggctcgtt gatgtcgatg atgacgacct gcggatcgcc gcgattcggc
      841 aacgtcagct tgagtcgcgt gtcgtacgag atcgcttcga gcttggccag cagcgtggtc
      901 ttgccgaact gcgtcagcgg atgcagcagg aagacggcga tgatcagcag gccgacgagc
      961 cagcggggcg cgccgatcag ccacgtggcg agcgtcccgg cggcgagacg cacggccaga
     1021 cgagctagcg gcgcgtccgc cggcgcagtg tcgccatcgc cagcgccgcg accgcgacgg
     1081 aaagcagtgc caatccgatc gacgaaagca cgggaatccc actcgggtcg gatgtcgacg
     1141 ccgcgacatt cgccttcccg tccggcctcg acggcaacag gacgcctttg atcaccagct
     1201 catcgacggg tcccgcgcct gccggcaagt gcgagctgtc gccggcgtag aaggccgaca
     1261 aggtgaaggc atcgatcgtc agccccgtca agcgaaagtc ggcgcggccg ttcgggtcga
     1321 gcacaccgga cagcggcgcg gcgatctttt ccggcggcgt ggccgggggc acggtgtcga
     1381 atggcggccc gcagaacatg ccggtgccgc gcacacaaag ctggaccgga ccctgcggcg
     1441 acggcggccc tccggatgcg accagcgcta cggtcaccgt ctgcccgaac acggcgatct
     1501 gtggcagcgc ggccagcgac agcgtcgtcg tcgcgcggtc tacggtgagc gtgaatgtct
     1561 gcaccgccgg cgcgttgcag ccgttgttcg ccgtgaccgt caaggtcgac gcacccgcgg
     1621 cctccggcgt gccactgatc agacccgagg
//
LOCUS       DATANS010000583         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_365844, whole genome shotgun sequence.
ACCESSION   DATANS010000583 DATANS010000000
VERSION     DATANS010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365844"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="VHT22_08580"
     CDS             complement(<1..1158)
                     /locus_tag="VHT22_08580"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX3137652.1"
                     /translation="MTTDRRDPAAPIPLFQCSVPAGARERLLATLDSGWLGYGPQCRL
                     LEERFTAGRGGWALATSSCTSALYLAGRLVRALAGESQPEIIIPSISFVASGMAFLQA
                     GVRPVIADVGADGLLLGEDGVRRALTPRTRALLAVHLYGQRCDELPALRALADEHGLL
                     LIEDCAHRIDLLDGTGPLGDLLCYSFNAVKELPGGEGGLLWGRDRRHEAWVRPVSNLG
                     LAVDTMQRTADLRHADYEFAAESGLKLRSNDVAAALVNAAIETLPESRAQRREQFQRY
                     DSLLAAIGAEVRPIERRDDDSCLMYVIRVAPEMRDPVRAAMAAAGIATSVHYPSLARH
                     PLFGNEQPDGCCGDEDKRIITLPTFARLTADDQRRVVDALAYALDGVRDRTP"
     gene            complement(1155..>1516)
                     /locus_tag="VHT22_08585"
     CDS             complement(1155..>1516)
                     /locus_tag="VHT22_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137653.1"
                     /translation="GDAAAGVLELGRVMVDAGKLVAYRSLLPPDYAGVAVDAGVALTD
                     FLFAALPLRVIHMTVITSNRLSVRAATTGGGKVIASGVQMRADGVEVPVTHIDFDRGA
                     WLRRAVQRKREPELQPR"
BASE COUNT          269 a          530 c          487 g          230 t
ORIGIN      
        1 aggcgtccga tcccgcacgc catccagcgc gtaagccaag gcatcgacga cgcgccgctg
       61 atcgtcggcg gtcagccggg caaacgtcgg cagcgtgatg atgcgcttgt cctcgtcgcc
      121 gcagcagcca tccggctgct cgttgccgaa cagcggatgc ctggcaagcg aaggataatg
      181 cacgctggtg gcgatgccgg cagccgccat cgcggcacgc accggatcgc gcatttccgg
      241 cgcaacccgg atgacataca tcaggcagct gtcgtcgtcg cgacgttcga tcggtcgcac
      301 ctcggcgccg atcgccgcga gcagactatc gtagcgctga aattgttcgc ggcgctgcgc
      361 gcggctttcg ggcagcgtct cgatcgccgc gttgaccagc gccgccgcga cgtcgttgct
      421 gcgcaacttc agcccggatt cggccgcaaa ctcgtaatcg gcatgccgca ggtccgcagt
      481 ccgctgcatc gtgtcgacgg cgagcccgag attcgacacc ggccgcaccc atgcttcatg
      541 acgccgatct cggccccaca gcaggccgcc ttcgccacca ggtagttcct tgacggcgtt
      601 gaagctgtaa cagagaaggt cgcccaatgg cccggttccg tcgagcagat cgatgcggtg
      661 cgcgcagtct tcgatcagca gcaagccatg ctcgtcggcc agggcgcgca gcgccggaag
      721 ctcgtcgcaa cgctgcccgt acaaatgaac cgccagcagt gcccgggtgc gtggcgtcaa
      781 cgcgcgtcga acgccgtcct cgcccagcag cagaccatcg gcgccgacgt cggcgattac
      841 cggccgtacg cccgcctgaa gaaacgccat gcccgacgcg acaaagctga tcgatggaat
      901 gatgatctca ggctgcgact caccggccag cgcgcgcacg aggcggccgg caaggtagag
      961 cgcactcgtg cacgacgacg tcgccagcgc ccagccgccg cggccggcgg tgaaacgctc
     1021 ctcgagcaag cggcattgcg gtccatagcc cagccatccc gaatcgaggg tcgcgagcaa
     1081 tcgctcgcgc gcgccggcgg gcacgctgca ctggaacagc gggattggtg cggcggggtc
     1141 tcttcgatca gtagtcatcg gggctgcaac tcgggctccc gcttgcgctg caccgcgcga
     1201 cgcagccagg cgccgcggtc gaaatcaata tgtgtgaccg ggacttcgac gccgtcggcg
     1261 cgcatctgca cgcccgaagc gatgaccttg ccgccgccgg ttgttgcggc gcgtaccgat
     1321 aagcggttgc tggtgatgac cgtcatgtgt atcacccgaa gcggcaacgc cgcgaacagg
     1381 aaatcggtca gcgccacgcc ggcgtctacc gcgacgccgg catagtcggg tggcaacagc
     1441 gagcgatatg cgaccagctt cccggcgtcg accatcaccc gcccgagctc cagcacacct
     1501 gctgcagcgt ctcctt
//
LOCUS       DATANS010000584         1835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_824343, whole genome shotgun sequence.
ACCESSION   DATANS010000584 DATANS010000000
VERSION     DATANS010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824343"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..568
                     /gene="tpx"
                     /locus_tag="VHT22_08590"
     CDS             68..568
                     /gene="tpx"
                     /locus_tag="VHT22_08590"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEX3137654.1"
                     /translation="MSTVTLGGNPVNVAGNFPKAGDAAPDFTLTGKDLKDVSLKDYAG
                     KKKVLNIVPSLDTPVCQTSTRKFNEKAGSLANTVVLVISADLPFAMKRFCEAEGINNV
                     TSLSTMRGRDFHAKYGVDVADGPLKGVTARAVVVLDENNKVKYSQLVPEIKNEPDYDA
                     ALAALK"
     gene            652..963
                     /locus_tag="VHT22_08595"
     CDS             652..963
                     /locus_tag="VHT22_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX3137655.1"
                     /translation="MTGTLDDFTMCDCTLCVKKNAVMYKVHESGLKILKGEDKLVLYR
                     WNTRVAQHYFCGACGIYTFHRKRAAPDHYGVNVYCLDGVDVSQVPVRRVDGKTMSVAA
                     S"
     gene            977..>1835
                     /gene="gcvPB"
                     /locus_tag="VHT22_08600"
     CDS             977..>1835
                     /gene="gcvPB"
                     /locus_tag="VHT22_08600"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX3137656.1"
                     /translation="MLIFELSQRERRNAVQMPPIASQPASIPVALRRATPPLLPQVSE
                     LDAVRHYTNLSRRNFSIDTHFYPLGSCTMKYNPRACNALAMLPQFLARHPSAPAATGQ
                     GFLACMFELQEMLMDVTGMAGVSLAPMAGAQGEFAGVAMIRAYHVARGDTARTEILVP
                     NAAHGTNPASAVMCGYTVREIPTDREGNVELAALAAAVGPHTAGLMLTNPSTLGVFER
                     SIIDIRKIVHDAGGLLYYDGANLNAILGKVKPGDMGFDVIHINLHKTFSTPHGGGGPG
                     AGPVGVATRL"
BASE COUNT          362 a          590 c          556 g          327 t
ORIGIN      
        1 ggccgacctt ctcgcggcgc cgcgcgcgct taactcttta atcccaattc aaccaaggag
       61 aatttctatg tctaccgtta ctctcggcgg caaccccgtc aatgtcgctg gcaactttcc
      121 gaaggcgggc gatgccgcgc cggacttcac cctgaccggc aaggacttga aagacgtgag
      181 tctcaaggat tacgcgggca agaagaaagt gctcaacatc gtacccagcc ttgatacgcc
      241 ggtatgccaa acctcgacgc gcaaattcaa cgagaaagcc ggcagccttg ccaacaccgt
      301 cgtgctcgtg atatccgccg acctgccatt cgcgatgaag cgtttctgcg aagccgaagg
      361 catcaacaat gtcacctcgc tctcgacgat gcgcggccgc gatttccatg cgaagtacgg
      421 cgtcgacgtc gccgatggcc cgctgaaggg cgtcactgcg cgcgcagtgg tcgtgctcga
      481 cgagaacaac aaggtcaagt actcgcagct cgtgccggaa atcaagaacg aacccgatta
      541 cgacgcggcg ctggccgcat tgaaatagcg caagacgcgg gcggccggaa catggttttt
      601 ccgagcggaa cgttcagggg ttcctgtcat tgcggcagcg tggagttcga agtgacgggt
      661 accctcgacg acttcacgat gtgcgactgt acattgtgcg taaagaagaa cgccgtcatg
      721 tacaaagtgc acgaatcggg cctgaagatt ttgaagggcg aagacaagct cgtgctctac
      781 cggtggaaca cgcgggtcgc ccaacattat ttctgcggcg cgtgcggcat ctatacgttt
      841 caccgcaagc gcgccgcgcc cgaccattac ggcgtgaacg tgtactgtct cgacggcgtc
      901 gacgtgtcgc aggtgccggt gcggcgcgtc gacggcaaga ccatgtcggt tgccgcaagc
      961 taacgcaagg cgcgccatgc tgatattcga attgtcgcag cgcgagcggc gcaacgccgt
     1021 gcagatgccg cccatcgcca gtcagcccgc gtcgattccg gtagcgctgc gccgcgccac
     1081 gccgccgctg ttgccgcagg tttccgagct cgacgcggtg cgccattaca cgaacctgtc
     1141 gcgtcgcaat ttttcgatcg acacccactt ttatccgctc ggctcgtgca cgatgaaata
     1201 caatccgcgc gcgtgcaacg cgctcgccat gctgccgcag tttctcgcgc gccatccatc
     1261 cgccccggcc gctaccgggc agggctttct cgcgtgcatg ttcgaactgc aggaaatgct
     1321 catggacgtg acggggatgg cgggtgtcag cctggcgccg atggccggcg cgcagggcga
     1381 attcgccggc gtggcgatga tccgcgcata ccacgtcgcg cgaggcgata ccgcgcgcac
     1441 cgaaatcctg gtgccgaacg cggcgcacgg caccaatccg gcgtccgccg tcatgtgcgg
     1501 ctacacagtc agggagattc cgaccgatcg cgagggcaac gtcgaactcg ccgcgctcgc
     1561 cgcggcagtc ggcccccaca ccgcagggtt gatgctgacc aatccgtcga cgctcggcgt
     1621 gttcgagcgc tcgatcatcg acatcaggaa aatcgtgcac gatgcgggcg ggctcctgta
     1681 ttacgacggc gccaacctca atgcgatact cggcaaagtc aaaccgggcg acatggggtt
     1741 cgacgtcatc cacatcaatc tgcacaagac gttttcgacg ccgcatggcg gcggcggccc
     1801 cggcgccggt ccggtcggcg tcgccacgcg cctgt
//
LOCUS       DATANS010000585         1604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_28525, whole genome shotgun sequence.
ACCESSION   DATANS010000585 DATANS010000000
VERSION     DATANS010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28525"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1604)
                     /gene="mutL"
                     /locus_tag="VHT22_08605"
     CDS             complement(<1..>1604)
                     /gene="mutL"
                     /locus_tag="VHT22_08605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX3137657.1"
                     /translation="LKEILENSLDAGARQIDVDLAGGGIKLIRVADDGDGIGREDLPL
                     ALARHATSKITTASDLEAITTLGFRGEALASIAAVSRLTLTSRLRGKEHAWRIEVDGG
                     EISATLPAALSDGTTVTVHELYFNTPARRKFLRTEATEYGHCEEAFRRAALSHPDVGF
                     TLQHNGSVKHRLLAQGRRARVDALLGEAFAAHAAPVDEPGPGLDLAGWVVRPAYATAT
                     REAQYLFVNGRFVRDRILSHALREAFRDVLHHDRQPAYALWLSIDPRIVDVNVHPTKS
                     EVRFRDSGAIHQFVRRAVEKALAATAAEQPAVSAAERLGAAAARIPPIARAGESVAGD
                     WRGAGASAQAVMTLGTAEPASFYARLFGQRTPPAGRPDLPDGSEHPLGFALAQLHGIY
                     VLAQNRDGLVLIDMHAAHERILYERLKTALDRAMPMQPLLVPVTFAADPLDVATADEN
                     AEILSALGFSLSVLGPSMLAVRGVPALLADADTVALARAVLADVREYGGSRVLTVQRD
                     ELLSTMACHGAVRAHRALGVAEMNAL"
BASE COUNT          250 a          558 c          556 g          240 t
ORIGIN      
        1 gcagcgcatt catttccgcg acgcccaacg cgcggtgcgc gcgcaccgcg ccgtgacagg
       61 ccatcgtcga caagagttcg tcgcgctgca cggtcaggac gcggctgccg ccatattcgc
      121 gcacgtcggc gagcaccgcg cgcgcaagcg cgacggtgtc ggcgtcggcg agcagcgccg
      181 gcacgccgcg caccgccaac atcgacggcc cgagcaccga cagcgagaac ccgagcgcgg
      241 acaggatttc cgcgttttcg tcggccgtcg ctacatccag cggatcggcg gcaaaggtca
      301 ccgggaccag cagcggctgc atcggcattg cgcggtcgag cgcagtcttg agcctctcgt
      361 agaggatgcg ctcgtgcgcg gcgtgcatgt cgatcaacac caggccgtcc cggttctgcg
      421 cgaggacgta aatgccgtgc aattgcgcca gcgcgaagcc cagcggatgc tcgctgccgt
      481 cgggcagatc gggccggccg gcaggcggcg tacgctgccc gaacagccgc gcataaaacg
      541 acgcaggctc cgccgtgccg agggtcatca ccgcctgcgc tgaagccccg gcgccgcgcc
      601 aatcgccggc gaccgattcg cccgcccgcg cgatcggggg aatccgcgcc gcggccgcgc
      661 cgagacgttc ggctgcggat acggcgggct gctcggccgc cgtcgcggcc agcgcttttt
      721 cgactgcccg tcggacgaac tgatggatgg cgccgctgtc gcgaaaacgc acttcgctct
      781 tggtcggatg gacattgacg tcgacgatgc gcgggtcgat ggaaagccac agcgcgtacg
      841 ccggctgccg gtcatggtgc agcacgtcac ggaacgcttc gcgcagggca tgcgacagga
      901 tgcggtcgcg cacgaagcgg ccgttgacga acaggtactg cgcttcgcgc gtcgccgttg
      961 cgtatgccgg ccgcacgacc cagccggcca gatcgaggcc cggcccaggc tcgtcgaccg
     1021 gcgctgcatg cgcggcgaac gcttcgccga gcagcgcatc gacgcgggcg cgccgtcctt
     1081 gcgcaagcag gcgatgcttg acgctgccat tgtgctgcaa ggtgaaaccg acatcgggat
     1141 gcgacagcgc tgcacggcgg aacgcctcct cgcaatggcc gtattcggtg gcttcggtgc
     1201 gaaggaactt gcggcgcgcc ggcgtgttga aatagagctc gtgcacggtc accgtcgtac
     1261 cgtcggagag cgccgccggc agcgtcgcgc tgatctcgcc accgtcgact tcgatgcgcc
     1321 aggcgtgctc tttcccgcgc aggcgcgacg tcagcgtcag gcgcgatacg gcggcaatgc
     1381 ttgccagcgc ctcgccgcga aagcccagcg tcgtgatcgc ctcgaggtcg ctggcggtgg
     1441 tgatcttgga tgtcgcgtgg cgcgccagcg ccagcggcag atcctcacgc ccgatgccat
     1501 ctccgtcgtc ggcgacgcga atcagcttga tcccgccgcc ggcgagatcg acgtcgattt
     1561 gccgcgcgcc ggcgtcgagg ctgttctcga ggatctcctt cagc
//
LOCUS       DATANS010000586         4265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_237228, whole genome shotgun sequence.
ACCESSION   DATANS010000586 DATANS010000000
VERSION     DATANS010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237228"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1564)
                     /locus_tag="VHT22_08610"
     CDS             complement(<1..1564)
                     /locus_tag="VHT22_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137658.1"
                     /translation="MPSGAWLDPYLAWAKVTEFKYVGGLPGDWIPILLELQGTTAQQF
                     AQTVRPMADIRVPSIFATPPHGIEQATFCSALVTRQLLENVESNELFRTQVKRFELGV
                     PVSAPFLTPQLTASTQLGVHAPTNAVVAVIDDGLAFAHQRFRDASGATRVRYFWNQDD
                     PQTTASYPFWGRELDNNAIDALLAASTHSGVVDEDALYRSATQQLAARRRRHGTHVMD
                     LACGMDPPKVAPTSPYIIAVQLPRSVVEDTSGAKLTPVVLEALHYILRRADDIAALEN
                     TTQLPVVVNLSYGLIAGPHDGSAPLEAAIDQLIALRPAPLRVVVPTGNAYLARCHSEF
                     RLPKPGTNPTIEELRWRLQPDDRAGSSMEIWLPFPTTAPPPTVQLRVTTPTGATSPWI
                     GPGGTWPWPSLGNVRFYANYPIAPPGSRQHIVLTMAPTAALDPALTPVAPSGTWRVEV
                     RYNGTPIVMHAWIQRGDTPFGYPLRGRQSRFDHADYLRFNRVGVLKKPVGALNEDDIP
                     PQSRVVRRGTINA"
     gene            complement(1671..2861)
                     /locus_tag="VHT22_08615"
     CDS             complement(1671..2861)
                     /locus_tag="VHT22_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX3137659.1"
                     /translation="MSGASGASGASGASGASIVSGASGASGASGASGASGASGASGAG
                     GYAAGNALVTDALIKHRDGIVGNWLGATTTPVQRALENPVNLQSWEPWVRAASIQFDL
                     LSNIYVTRHAETFTYAGPTGTVTKSSNRSVTVWHMRVTNTGPGNSPRATSSRLATIIR
                     PTPGIFKQQLNFLDTYADLREDRASEIIAQMTPPVAFWSSIAYLHPDRTKKTLELLDV
                     ALGLVGAVEQRIKHALACRRPNEYSAQVQPMILTPGHGSFPSGHATEAHMIARILWEL
                     RGGTPGDALGVELMRQAARVAANRTVAGLHFPVDSCCGQMLGLTLARYFVRRAKGNTG
                     LRAWRFDGERFPGNLDFDFNNLYDTGTALQKSASYVDSVPTTLPVASPMLKWLWYQAA
                     AEWV"
     gene            complement(2903..>4265)
                     /locus_tag="VHT22_08620"
     CDS             complement(2903..>4265)
                     /locus_tag="VHT22_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137660.1"
                     /translation="VVSADVRDQLTPVLDADIEDLGACYVKHVNEPVRAYRVGPPGPR
                     PVIEPGSAAMPELRPTVAVIPFTARGGDASHQVLGEVLADEIISALSRAAELNVISRL
                     STTVFRGREASLGEVGGYLNATYVLSGAYLVSGNQFNLSAELADAKSGSVVWAKSLKG
                     QVTGIVSGKDGLIDKAVAGVSSAIMARELLRAQSQPLPTLESYTLLMGAIALMHRLSL
                     QDFDRAKQMLQTLTERAPRQAVPWAWMAKWHVLRVQQGWSQDLALDARLAMDCTRRAL
                     DTDPHCSLALVIDGLVHTNLLKQLDTAQERYELALHANPNDSLAWLLKGTLHAFRGEG
                     KAAVEGTQRALRLSPLDPLRYYYDSLAATAALSAGQYERAIKLAEHSLRANRTHTSTV
                     RALAISQWLLGRQEDARKTVGELMRLEPALTVEKWLERSPTSPYETGKQWSGALRNAG
                     VPM"
BASE COUNT          723 a         1414 c         1372 g          756 t
ORIGIN      
        1 ttgcgttgat cgtcccgcgc cgcaccaccc ggctttgggg cgggatgtcg tcctcgttca
       61 acgcgcctac cggctttttc aacactccaa cccgattgaa gcgcaggtag tcggcgtgat
      121 cgaagcggga ctgacggccg cgcagcggat agccgaacgg tgtgtcgccg cgctgaatcc
      181 aggcgtgcat cactatcggc gtgccgttgt atctgacctc gacacgccat gtcccggaag
      241 gcgcaaccgg agtcagcgcc ggatccagcg cagcggtcgg cgccatggtc aatacgatgt
      301 gctgccggct gccgggcgga gcgatcgggt agttcgcgta gaaccgcacg ttcccgagcg
      361 acggccatgg ccaagtgccg ccgggtccga tccagggact cgtcgcaccc gtcggcgtcg
      421 ttacgcggag ctggaccgtc ggcggcggcg cggtggtcgg aaacggcagc cagatttcca
      481 tcgagctgcc ggcgcgatcg tcgggctgca accgccacct gagctcctcg atggtgggat
      541 tcgtcccggg cttcggcaat cggaactcgc tatggcagcg cgcaagataa gcgttgcccg
      601 tcggaacaac gacgcgcagc ggcgccgggc gcagtgcgat cagctgatcg atcgccgctt
      661 ccagcggcgc cgagccgtcg tgcggcccgg cgatcaggcc gtagctcaag ttgacgacga
      721 ccggcaactg ggtagtgttt tccagcgccg cgatgtcatc ggcgcggcgg agaatgtagt
      781 gcagcgcctc gaggacgacg ggcgtcaact tcgccccgga agtgtcttcg accaccgatc
      841 gcggcaactg cacggcaatg atgtaaggcg acgtcggtgc gaccttcggc ggatccatgc
      901 cgcaggccag gtccatgacg tgcgtgccat ggcgtcgccg gcgtgccgcc agctgctgcg
      961 tcgccgatcg gtacaacgcg tcttcgtcga cgacccccga atgcgtgctc gcggcgagga
     1021 gcgcgtcgat ggcgttgtta tccagctcgc ggccccagaa cggatacgac gcggtcgtct
     1081 gcggatcgtc ctgattccag aaatagcgga cgcgcgtcgc accggatgcg tcgcgaaacc
     1141 gctggtgtgc gaatgccagc ccgtcgtcga tgacagcgac gacggcgttc gtcggcgcgt
     1201 ggacgccgag ttgcgtgctt gctgtgagtt gcggcgtcag aaatggcgcc gataccggca
     1261 cgccgagttc gaagcgcttg acctgcgtcc ggaagagctc attgctctcg acgttctcga
     1321 gcaattgcct ggtcaccagg gccgagcaga acgtcgcctg ctcgatgccg tgcggtggcg
     1381 tcgcgaatat cgacggcacg cgaatgtctg ccatcgggcg caccgtttgc gcgaactgct
     1441 gggcggtcgt cccctgaagc tccagcaaaa tgggaatcca gtcgcccggc aagccaccga
     1501 cgtacttgaa ctcggtgacc ttcgcccagg cgagatacgg atcgagccac gcgccgctgg
     1561 gcatcgctcg cggttctgca cccgagcgcg ccttgcttga attgcgtctt ttagctattt
     1621 gcgccatcgc ggaaccccga atcggtcatg acaattgcgg catgaggacg tcacacccat
     1681 tcggccgcgg cctggtacca gagccatttc agcatcggtg aagcgacggg caacgtcgtc
     1741 ggcaccgaat cgacgtagga cgcggatttt tgcagtgccg tgcccgtgtc atagagattg
     1801 ttgaaatcga agtccagatt gccgggaaag cgctcgccat cgaaacgcca ggcgcgcagg
     1861 cccgtgttgc ctttggcacg acgcacgaag tatcgcgcca gcgtaaggcc cagcatctgt
     1921 ccgcagcagc tatcgaccgg aaaatgcagc cctgcaaccg tgcgattggc ggcgactctc
     1981 gccgcctggc gcatcagctc gacgcccagg gcgtcgccag gcgttccgcc gcgaagctcc
     2041 cacagaatgc gcgcgatcat gtgcgcctcg gtcgcgtgtc cgctgggaaa cgagccgtgg
     2101 cccggagtga ggatcatcgg ctgtacctgc gccgaatact cgttgggccg gcggcaggcg
     2161 agcgcgtgct tgattctctg ctcgacggcg ccgacgagcc ccagcgcaac gtcgagcagc
     2221 tccagcgtct tcttggttcg atccgggtgc aggtacgcga tcgagctcca gaatgcgacc
     2281 ggcggagtca tctgggcgat gatctcggag gcgcgatcct cgcgcaggtc cgcgtacgta
     2341 tccaggaagt tgagttgctg cttgaagatg cccggcgtcg gccgaatgat ggttgcgagc
     2401 ctgctcgacg tggctctcgg cgaatttccc ggtccggtgt tggtcacgcg catatgccag
     2461 acggtgaccg agcgattcga gctcttggtg accgtgccgg tcgggccggc ataagtaaaa
     2521 gtctcggcgt gtcgcgtcac atagatgttg gacagcagat cgaactgaat cgacgctgcc
     2581 cgtacccacg gctcccaact ctgcaggttt accggatttt cgagggcgcg ctggaccggg
     2641 gtggtcgtcg cgcccagcca gttgccgacg atgccatcgc gatgcttgat cagcgcatcg
     2701 gtgaccagcg cgttgccggc agcgtaacct ccggcgccgc tcgcgccgct cgccccactg
     2761 gccccactcg cgccgcttgc cccgcttgcc ccactggcac cgctgacgat gctggcgccg
     2821 ctcgcgccgc ttgccccgct cgctccactt gctccactca tggcagaacc tcccttagac
     2881 ccaccaccac ccaaaaacca tcctacatcg gcacccctgc gttgcgcagc gcgccggacc
     2941 actgttttcc tgtttcgtaa ggactggtgg gcgagcgctc gagccacttc tccacggtaa
     3001 gagcgggctc gagtcgcatc agctcaccga cggtctttcg cgcgtcctcc tggcgtccaa
     3061 gcagccactg agaaatcgcc agcgcgcgga ctgtcgaggt atgtgtccgg ttcgcgcgca
     3121 atgaatgctc ggcgagcttg atcgcgcgct cgtattgccc tgccgaaagc gctgcggtcg
     3181 cagccagcga atcgtagtaa tagcgcagcg gatcgagcgg cgaaagtcgc agggcccgtt
     3241 gcgtgccttc gaccgccgcc ttgccttcac cccggaacgc gtgcagcgtt cccttgagca
     3301 gccatgccag cgaatcattc gggttggcgt gcaacgccag ctcgtaccgt tcctgcgcgg
     3361 tgtcgagttg cttgagcaaa ttcgtgtgca ccagcccgtc gatgacgagc gccagcgagc
     3421 agtgcggatc ggtatcgagc gctcttcgcg tgcaatccat cgccaaacga gcgtccagcg
     3481 ccagatcctg cgaccagccc tgctgcacgc gcaggacgtg ccatttggcc atccacgccc
     3541 agggaaccgc ttgccgggga gcgcgttcgg taagcgtttg cagcatctgc ttcgcgcggt
     3601 cgaaatcctg cagcgaaagc cggtgcatca gcgcgatcgc gcccatcaac aatgtgtaac
     3661 tttcgagcgt cggcagcggc tgcgactgcg cgcgcaacaa ctcccgcgcc atgatcgcgg
     3721 agctcacccc cgcgacggcc ttgtcgatca gcccgtcctt gccgctgacg atgccggtga
     3781 cctggccctt gaggctcttg gcccagacga cactgccgga cttggcgtcg gccagttcgg
     3841 cgctcaggtt gaattggttg cccgacacca ggtaggcgcc ggagagcaca tacgtcgcat
     3901 tgaggtaacc gcccacttcg cccagcgacg cttcgcgccc gcggaagacc gtcgtcgaca
     3961 ggcgcgatat cacgttcaac tctgcggcgc gcgacaacgc cgagatgatc tcgtcggcca
     4021 gcacttcgcc cagtacctgg tgtgaggcgt ctccgccccg ggcggtgaac ggaatcaccg
     4081 ccaccgtcgg gcgcagctcg ggcatcgcgg cgctgccggg ctcgatcacc ggacgcggcc
     4141 ccggcggacc gacgcgatag gcgcgcaccg gctcattgac gtgcttgacg taacacgcgc
     4201 cgagatcctc gatgtcggcg tccagcaccg gggtcagttg gtcccgaacg tccgcggaga
     4261 caacg
//
LOCUS       DATANS010000587         1851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_237234, whole genome shotgun sequence.
ACCESSION   DATANS010000587 DATANS010000000
VERSION     DATANS010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237234"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="VHT22_08625"
     CDS             complement(<1..1252)
                     /locus_tag="VHT22_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3137661.1"
                     /translation="MGLERYREKRNFRATPEPRGRVARSKANELSFVIQKHAASHLHY
                     DFRLELNGVLLSWAVPKGPSLDPHDKRLAMHVEDHPLEYGGFEGIIPPRQYGSGTVMV
                     WDRGSWHPIGDPVAGYQKGHLKFELDGEKLKGGWALIRTHSSKYGGKSGKQAWLLIKE
                     SDEYAKEGNAARIVDEEPDSVVSGRSLEEIARDKTNEWHSNRSVAANVRGGALAEVVS
                     AKSRKAAKPSAGKLSRSMSAGGENGKNKAPQSPASAAGAKKARMPAALSPTLATLVDS
                     APTGDDWIHEVKFDGYRMVSRIDHGDVRIYSRNGKEWTAALPPVVASLQRLDVDQAWL
                     DGEIAVADAQGLTSFQQLQNALSDPRAKKISYFIFDLPYQDGYDLRGVALTERKQLLR
                     ALVGKSDPVLRYSVDVQGSGAEFFE"
     gene            complement(1271..>1851)
                     /locus_tag="VHT22_08630"
     CDS             complement(1271..>1851)
                     /locus_tag="VHT22_08630"
                     /inference="COORDINATES: protein motif:HMM:NF014755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX3137662.1"
                     /translation="PADRGEKVYALLRETLRATGRVAVAQVVIRTTEHLAAVVPQGRA
                     LMLVTMRHADEIRGASGFELPAEGLKSAGVSSKEVDLAKRLVDDMTEHWKPEEFKDTY
                     HEDLMRRIHEKIKAGETKEITKRTAEDGEEPRSAKIIDLAALLKESLDKGGTRRKPAE
                     ARRSKAEIAKPALRMVAASRGSGRSTAKRKRA"
BASE COUNT          264 a          640 c          575 g          372 t
ORIGIN      
        1 gctcgaagaa ttcggctccc gagccttgca cgtcgacgct gtagcggaga accggatccg
       61 acttgccaac cagtgcccgc agcaattgct tgcgctcggt caatgcgacg ccgcggagat
      121 cgtagccgtc ctgatagggc aagtcgaaga tgaaataact gatcttcttc gcacgcggat
      181 cggacagcgc gttttgcagc tgctgaaagc tggtcaagcc ttgcgcgtcg gcgacggcaa
      241 tctcgccgtc gagccacgcc tggtcgacat ccagcctctg cagcgaggca acgaccggcg
      301 gcaacgctgc ggtccattcc ttcccgttgc gcgagtagat gcgaacgtca ccgtggtcga
      361 tccggctgac catgcgatag ccgtcaaact tgacttcgtg aatccagtcg tcgccggtcg
      421 gcgcgctgtc gaccagggta gccagcgtcg gcgatagcgc cgcgggcatc cgggccttct
      481 tcgcgccggc cgccgacgcc ggagactgcg gtgccttgtt ttttccgttc tcgccgccgg
      541 cgctcatcga gcgcgacagt ttgccggccg acggcttcgc ggccttgcgc gatttggccg
      601 agacgacctc ggccagcgcg ccgccgcgca cattcgccgc gaccgaacgg ttcgaatgcc
      661 attcgttcgt cttgtcgcgc gcgatctctt ccaggctgcg gccggacacg acgctgtcgg
      721 gctcctcgtc gacgatgcgg gccgcgttgc cttccttcgc gtactcgtcg ctctccttga
      781 tcagcagcca cgcctgcttg cccgatttgc cgccgtactt gctgctgtgc gtgcggatca
      841 gcgcccagcc gcccttgagc ttttcgccgt cgagctcgaa cttgagatgg cccttctgat
      901 atccggccac cgggtcgccg atcggatgcc agctgccgcg atcccacacc atcaccgtgc
      961 ccgatccgta ctggcgcggc ggaatgatgc cttcgaagcc gccgtattcg agcggatgat
     1021 cttcgacgtg catcgccagc cgcttgtcgt gcggatcgag gctcgggccc ttgggcaccg
     1081 cccagctcag caggacgccg ttcaactcca gccggaaatc gtagtgcagg tgactggccg
     1141 catgtttctg gatgacgaag ctcaactcgt tcgccttgct gcgagcgacg cggccgcgcg
     1201 gctcgggcgt cgcccgaaaa ttgcgcttct cgcggtagcg ttccagtccc atgcgtgtcg
     1261 agcgcgacgc tcaggcgcgc ttgcgcttgg cggtcgagcg gcccgatccg cgcgatgcgg
     1321 cgaccatgcg cagcgccggc ttggcgatct cggccttcga acggcgcgcc tcggccggtt
     1381 tgcgtcgcgt cccgcccttg tcgagactct ccttgagcaa ggccgcgagg tcgataatct
     1441 tcgccgagcg gggctcctcg ccgtcttccg ccgtccgctt ggtgatctcc ttggtttcgc
     1501 ccgccttgat cttctcgtgg atgcgacgca tcaggtcttc gtgataggta tccttgaact
     1561 cctccggctt ccagtgctcg gtcatgtcgt caaccaggcg cttggcgaga tcgacttcct
     1621 tgctgctgac gccggcgctc ttcaatcctt ccgccggcag ctcgaacccg ctggcgccgc
     1681 ggatctcgtc ggcatggcgc atcgtgacga gcatcaacgc acgcccctga gggacgacgg
     1741 ccgccaggtg ctcagtcgtg cggatgacca cttgcgccac ggcgacacgc ccggtcgccc
     1801 gcaaggtttc gcgcagcagc gcgtagacct tttcgccgcg atcggctggc g
//
LOCUS       DATANS010000588         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_198849, whole genome shotgun sequence.
ACCESSION   DATANS010000588 DATANS010000000
VERSION     DATANS010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198849"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VHT22_08635"
     CDS             <1..551
                     /locus_tag="VHT22_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3137663.1"
                     /translation="LREACEQARAWQSAGLPRLRVGINVSTAELRAKDFVEGVRNILA
                     ETGMDPRFLELELTEAFLLENPQATGPVLRALKDTGAQLALDDFGTGYSSLGCLKRFP
                     IDTLKIDQSFVRDLATDPDDAGIVSAVIAIGRSLEKRVVAEGVETREQLAFLREKNCP
                     DGQGFFFSPAVPAAGMVELLGG"
     gene            complement(564..1928)
                     /gene="mpl"
                     /locus_tag="VHT22_08640"
     CDS             complement(564..1928)
                     /gene="mpl"
                     /locus_tag="VHT22_08640"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEX3137664.1"
                     /translation="MHLHILGICGTFMGGIAAIARQSGHTVTGCDANVYPPMSTQLAS
                     LGIALTEGYDAAQLDTVAVDADVFVIGNAISRGNPLLEAILDTNRRYISGPQWLAENV
                     LLDKWVLAVTGTHGKTTTSAMLAWILDRAGLQPGFLIGGVATDLGVSARRSDSAFFVI
                     EGDEYDTAFCDKRSKFVHYRPRTAILNNLEYDHADIFPDLAAIETQFHHLVRTIPHAG
                     RLIVNGGEESLKRVLARGCWSEVERFGFAAVADTGADWTIAVDGTIKLGGAPQGVLAP
                     FALLGRHNQLNALSAIAAARHAGVPVAQSLVALAAFRGIARRLEVRGSINGVTVYDDF
                     AHHPTAIAATLAALRRNVGSQRIIAIVEPRSNTMKLGRMNARLPASLEQADRVYCYAA
                     NLGWDAAAVLAPLGDRARVASDLDGLIDAVVRDARPGDHLLVMSNGGFGGIHGKLLAR
                     LAGS"
     gene            complement(1940..>2164)
                     /locus_tag="VHT22_08645"
     CDS             complement(1940..>2164)
                     /locus_tag="VHT22_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137665.1"
                     /translation="LLAHLGVTATIADNDDDYVAIAVRLAHDRPWRDAVAAEILAALP
                     ISGLADADRYTRSLESAYERALALKARQPD"
BASE COUNT          357 a          695 c          729 g          383 t
ORIGIN      
        1 tattgcgcga agcgtgcgag caggcccgtg cctggcaaag cgccggcctg ccccggttgc
       61 gcgttggaat caatgtttct acggcggaat tgcgtgcgaa ggatttcgtc gaaggcgtac
      121 gcaacatcct ggccgaaacc ggcatggatc cacgctttct ggagctcgag ctgaccgagg
      181 ccttcctttt ggaaaatccg caggcgaccg gaccggtgct gcgggcactc aaggacacag
      241 gcgcgcagct cgcgctcgat gacttcggca ccggatattc gagcctgggc tgcctgaagc
      301 gttttccgat cgatacgctg aagatcgatc agtcgttcgt gcgagatctc gccaccgacc
      361 cggacgacgc cggcatcgtc agcgcggtga tcgccatcgg aagaagtctg gagaagcggg
      421 tcgtcgccga aggcgtggaa acgcgcgagc agctcgcatt cctgcgtgaa aagaactgcc
      481 cagacgggca gggctttttt ttcagcccgg cggtgcccgc agcgggtatg gtcgagttgc
      541 tgggaggctg agggcggatc gcatcagctg ccggccagtc gcgccagcag cttgccgtgt
      601 atgccgccga acccgccgtt gctcatcacc agcaggtggt cgccgggacg cgcgtcgcgc
      661 acgactgcgt cgatcaggcc atcgagatcg ctcgcgactc gggcccgatc gcccagcggc
      721 gccaggacgg ctgccgcgtc ccaacccagg ttggctgcgt agcaataaac acggtcggct
      781 tgctccaggc ttgccggcag ccgcgcgttc atccttccca gcttcatcgt attcgatcgc
      841 ggctcgacga tggcgatgat ccgctggctg ccgacgttgc gccgcagcgc ggccagggtc
      901 gcggcgatcg ccgtcggatg gtgcgcgaaa tcgtcgtaga cggtgacccc gttgatgctg
      961 ccgcgcactt cgaggcgccg ggcgatgccg cgaaacgccg ccagcgcgac gaggctttgt
     1021 gcgaccggca cgccggcgtg cctggcggcc gcgatcgccg acagcgcatt gagctgattg
     1081 tgccgcccga gcagcgcgaa cggcgcgagc actccctgcg gggcgccgcc tagcttgatc
     1141 gtgccgtcga cggcgatcgt ccagtcggcg ccggtgtcgg caaccgccgc gaagccgaat
     1201 cgctcgactt cgctccagca gccgcgcgcg agcacgcgct ttagactttc ttcgccgcca
     1261 ttgacgatca gccggccggc gtgcgggatc gtccgcacca gatgatggaa ttgcgtctcg
     1321 atcgccgcga gatcgggaaa aatgtcagcg tggtcatact cgagattgtt cagtatcgcg
     1381 gtgcgcggcc ggtagtggac gaacttcgag cgtttgtcgc agaacgcagt atcgtattcg
     1441 tcgccctcga tgacgaaaaa cgcgctgtcc gagcggcggg ccgagacgcc gaggtcggtc
     1501 gcgacgccgc cgatcaggaa gcccggctgc agacccgccc ggtcgagtat ccaggcgagc
     1561 atcgcgctgg tcgtcgtctt gccgtgtgtt cccgtcaccg ccagcaccca tttgtccagc
     1621 agcacgtttt cggcgagcca ttgcggcccc gaaatatagc gccggttggt atccagtatt
     1681 gcctcgagca gcgggttgcc gcgggagatc gcattgccga tgacgaacac gtcggcgtcg
     1741 acggcgaccg tatcgagctg cgccgcgtcg tagccctcgg tcagtgcgat gccgaggctc
     1801 gcaagctgcg tgctcatcgg cggatagacg ttggcgtcgc aaccggtgac cgtgtggccg
     1861 ctctgccgcg cgatcgcggc gatcccgccc atgaacgtcc cgcagatgcc gaggatgtgc
     1921 agatgcatgc gtttcgcttt cagtcgggct ggcgcgcctt gagcgccaac gcgcgctcgt
     1981 acgcgctttc gaggctgcgc gtataacggt cggcatcggc aagacccgag atcggcagcg
     2041 ccgcgagtat ttccgctgcg accgcatcac gccatggacg gtcgtgcgcg agtcggacgg
     2101 cgattgccac ataatcgtca tcgttgtccg cgatcgtcgc ggtgacgccg agatgggcga
     2161 gcag
//
LOCUS       DATANS010000589         2754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_408396, whole genome shotgun sequence.
ACCESSION   DATANS010000589 DATANS010000000
VERSION     DATANS010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408396"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VHT22_08650"
     CDS             complement(<1..467)
                     /locus_tag="VHT22_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137666.1"
                     /translation="MASARELLEQADALMRRNRGRSGADIPVLTDVVAGVPANDEVEI
                     DVPVLTDVVEAGAVDDADLPTAVQPSMPLEPAEADQAPPPLQADSVAIMQSADAIPVE
                     LPMDGDTSDWLVMDTIDPSTHSITGPGPDTLAVVPPVTLRAEPEPEPEPEPEP"
     gene            complement(467..892)
                     /locus_tag="VHT22_08655"
     CDS             complement(467..892)
                     /locus_tag="VHT22_08655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEX3137667.1"
                     /translation="MTSIDFYTHVSDRFDVVAKLVAKAFAQHGSVRVLTRDAESTEAL
                     DRMLWIQPAIGFLPHCRIGSRLAAETPIWIDHLLEHQGPAAVLINLHDAPPPFFSRFE
                     RLAEVVGADEAGKAAARERFRFYRERGYELRTHNLSERG"
     gene            complement(945..2438)
                     /locus_tag="VHT22_08660"
     CDS             complement(945..2438)
                     /locus_tag="VHT22_08660"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX3137668.1"
                     /translation="MEFTIKSGSPEKQRSACVVVGVFDNRKPSLSAELIDRASNGYIS
                     EIIRRGDMEGKLGATLLLHNVRGTLADRVLLVGLGKERDFREREFRSAIRAAVRLLNE
                     TGSYEAVVYLTEEKVKRREVAWRVEHAVVVAMESVYRFDQMKSQPADVRRPLRKLTLS
                     VPQRSDLTAGEAAAARGLAIAHGMDLARDLGNLPGNICTPAHLAERAQALAQELGFKC
                     EVFERGKLEELKMGSFLSVTNGSDQPPKFIVLEYFGGPKKQKPLVLVGKGITFDSGGI
                     SLKPGADMDQMKFDMCGAASVLGAFRAVAELKAKVNLIGLVPTCENMPNGRATKPGDV
                     VRSMSGQTIEVLNTDAEGRLILCDALTYAERYEPAAVVDIATLTGAMVIALGHVASGL
                     FSNSDSLGRALLNAGEDAFDRAWQLPLWDDYQEGLNSNFADFANIGSRAGGSITAACF
                     LSRFAKKFDWAHLDIAGVAWKEGKEKGATGRPVPLLTTWVLSQETAP"
     gene            2575..>2754
                     /locus_tag="VHT22_08665"
     CDS             2575..>2754
                     /locus_tag="VHT22_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEX3137669.1"
                     /translation="MALFLVLLGILFTNLVLRLLARAAGGTVAPEGVLALLGFNALFY
                     FNILLSVALFLTVLLT"
BASE COUNT          449 a          893 c          877 g          535 t
ORIGIN      
        1 tcgggttcgg gttcgggctc gggttcgggt tcgggttcgg ctctgagcgt gaccggtgga
       61 acgaccgcga gcgtatccgg gccggggccg gtaatgctgt gcgtcgacgg gtcgatcgta
      121 tccatcacca gccaatcgga cgtgtcgcca tccatcggca actcaaccgg gattgcgtcg
      181 gccgattgca tgatcgccac gctgtcagcc tgcagtggcg gcggtgcttg gtctgcctct
      241 gcgggttcca gcggcatcga aggctgcacc gccgtcggca gatccgcgtc atcgacggcg
      301 cccgcctcaa ccacatcggt gagcacgggc acatcgatct cgacctcgtc gttcgcggga
      361 acgccggcga ccacatcggt gagcaccgga atgtcggcac cgctgcggcc gcggttgcgt
      421 cgcatcagcg cgtcggcctg ctcgagaagc tcgcgtgcgc tcgccatcag ccgcgctcgc
      481 tcaggttatg cgtgcgaagc tcgtagccgc gttcgcggta gaaacgaaag cgctcgcgcg
      541 ccgcggcctt accggcttcg tcggcaccga ccacttcggc aaggcgctcg aagcgggaga
      601 aaaacggcgg cggcgcgtcg tgcagattga tcagaaccgc cgccggcccc tgatgttcca
      661 gcaggtggtc gatccagatc ggcgtttcgg ccgcgagccg gctgccgatc cggcaatggg
      721 gcaggaagcc gatcgccggc tggatccaca gcatccgatc gagcgcctcg gtcgactcgg
      781 catcgcgggt caggacccgc acgctgccgt gctgcgcaaa cgccttggcg accagcttgg
      841 cgacgacgtc gaagcgatcg ctcacgtgcg tgtagaagtc gatcgaggtc atcgaagcat
      901 ccgccggaga attcctcgtg acgcgcgcta aaggctcgcg cgggctacgg ggcggtctcc
      961 tgcgacagca cccacgtggt gagcagcggc accggacggc cggtcgcgcc cttttccttg
     1021 ccttctttcc atgccacgcc ggcgatgtcc agatgcgccc agtcgaattt cttggcgaac
     1081 cgggatagaa agcatgccgc cgtgatgctg ccgccggcac gactgccgat gttggcgaag
     1141 tcggcgaaat tgctgtttag cccctcttga taatcatccc atagcggcag ctgccaggcg
     1201 cggtcgaacg cgtcttcgcc ggcattgagc agcgcccgtc cgaggctgtc gctgttgctg
     1261 aacagaccgg aggcgacgtg gccgagtgca atgaccattg caccggtcag ggtggcgatg
     1321 tcgacgacgg ccgccggctc gtaacgctcg gcatacgtca gcgcgtcgca aaggatcaag
     1381 cggccttcgg cgtcggtatt caacacctcg atggtctgcc ccgacatgct gcggacgacg
     1441 tcgcccggct tggtcgccct gccgttgggc atgttctcgc aggttggcac gaggccgatc
     1501 aggttgacct ttgccttgag ctcggcgact gcacgaaacg cgcccagaac gctggctgcg
     1561 ccgcacatgt cgaatttcat ctgatccatg tcggcgccgg gcttgagcga gatgccgccg
     1621 ctatcgaagg ttatcccctt gccgaccagc accagcggct tttgcttttt cggaccgccg
     1681 aagtactcga gcacgatgaa cttcggcggc tgatcgctgc cgttggtcac cgacaggaac
     1741 gatcccattt tcagctcttc gagcttgccg cgctcgaaga cctcgcattt gaagcccaat
     1801 tcctgcgcca gcgcctgcgc gcgctcggcc aagtgagcgg gggtacagat gttgcccggc
     1861 aggttgccca ggtcgcgcgc caggtccatg ccgtgggcga tcgcgagccc gcgcgccgca
     1921 gcggcctcgc cggcagtgag gtcgctgcgc tgcggcaccg acagcgtcag ctttcgcagt
     1981 gggcggcgca cgtcggccgg ctggcttttc atctggtcaa agcggtagac ggactccatt
     2041 gcgacgacaa ctgcgtgctc gacccgccag gcgacttccc tgcgcttgac cttttcctcg
     2101 gtaagataga cgactgcctc gtacgacccg gtttcattaa gcaggcggac ggcggcgcgg
     2161 atggcgctgc ggaattcgcg ctcgcgaaag tcgcgctcct tgcccaggcc gaccagcagg
     2221 actcgatcgg ccaacgtccc gcggacgttg tgcagcagca aggtcgcgcc gagcttgccc
     2281 tccatgtcgc cccggcgaat gatctcgctg atataaccgt tcgaggcccg gtcgatcagt
     2341 tcggccgaca ggctcggctt gcggttgtcg aatacgccga cgacgacgca agcgctgcgc
     2401 tgcttttcgg ggctgccgct ttttatggta aattccatcg ccttccttcg aacgaaaaat
     2461 ttgcctttag gcggtacgaa ccgcattatc ggcaaatcgg cctgaaaaag tcaaaatatt
     2521 tccgtcgtca tggtcttccg gcgcagcctg gtacgagaac tgaccgcgac agcggtggca
     2581 ctcttcctgg tattgctggg gatactcttc accaatctgg tgctgcgcct gctggcccgc
     2641 gctgccgggg gaaccgtggc tcccgaagga gttctggctc ttttgggctt taatgccctc
     2701 ttctacttca acatcctcct gtcggttgcg ctgttcctga ccgtcctgct gacg
//
LOCUS       DATANS010000590         5563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_784624, whole genome shotgun sequence.
ACCESSION   DATANS010000590 DATANS010000000
VERSION     DATANS010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784624"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="VHT22_08670"
     CDS             <1..1120
                     /locus_tag="VHT22_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX3137670.1"
                     /translation="ALAAANLAFGATPVGADYRRLLILIELKGGNDGLNTLVPFADPA
                     YYALRPKLAIARDSVIQLSDAVGLHPALEPLLPLWRARELAVLQGVGYPEPNLSHFRS
                     IEIRDTASGSHQYLQDGWLTRTFALAPTPADFAADGVIVGSPDLGPLSGGGTRAIALA
                     NTEQFLRQARFATGEGQTRNKALQHILKVEADIVQAAARLKSDYAFKASFPQGAFGNA
                     IRTACQVVANRPGVAVVRVTQTGYDTHSGQAPTQARLLGELAGGVVALKNALGEVKRW
                     DDTLVLTYAEFGRRPKENLSNGTDHGTASAHFVLGGRVAGGLYGEQPSLERLSGDGNT
                     GYSIDFRSVYATALDRWWNVPSTGPLGGRFTPLPLLKT"
     gene            complement(1117..1917)
                     /locus_tag="VHT22_08675"
     CDS             complement(1117..1917)
                     /locus_tag="VHT22_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX3137671.1"
                     /translation="MTPTLVFDIETVPDIAGLRRLNRLPATLSDAEVATWAAQQRRAA
                     TGGDFLPLHLQRVVAIACGLREGSTFRVWSLGEASDAEPELIRRFFDGIDRYTPQLVS
                     WNGGGFDLPVLNHRALVHGISAARFWDWGDDDREFKFNSYLGRYHTRHLDLMDVLSMY
                     QPRATAGLDAIARLCGFPGKLGMDGSEVHAAFEAGKIDEIRCYCETDVMNTYLVYLRF
                     QLLRGTLSAAQYSTELSFAREKLAATGAPHWREFIAAWDANAHAAGGV"
     gene            complement(1966..2862)
                     /gene="cysM"
                     /locus_tag="VHT22_08680"
     CDS             complement(1966..2862)
                     /gene="cysM"
                     /locus_tag="VHT22_08680"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HEX3137672.1"
                     /translation="MASVYKTIEQTVGNTPLVELQRMAKASGNVVLVKLEGNNPAGSV
                     KDRPALSMVAQAEKRGTIKPGDTLIEATSGNTGIALAMAAAMRGFRMILVMPEHLSLE
                     RRQTMSAFGAEIVLTPKAGGMETARDVAERMRDDGKGIILDQFANPDNPLAHYEGTGP
                     EIWRDTGGRITHFVSSMGTTGTIMGVSRYLKEQNPTIAIIGCQPDEGSQIPGIRKWPE
                     AYLPKIFDRKRVDRIENVSQADAEATARRLAREEGIFVGISSGGACAVALRIAEEVRH
                     ATIVFVACDRGDRYLSSGVFPA"
     gene            complement(2986..3870)
                     /locus_tag="VHT22_08685"
     CDS             complement(2986..3870)
                     /locus_tag="VHT22_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX3137673.1"
                     /translation="MKRLLLLLVTVLFSALALAAVNINTANLAELEALPEIGPVKAQA
                     IIDYRNANGPFKSPEDVMKVSGIKEGTYGKIKGLISVSGASTPLPATAPAKAVAPAPS
                     AAAAPAKAAAPAPAPAPAPAAKTASPAAIPPAAGAAGAASGTDAAAAKDAKSKDAKMT
                     KKEKAEADKAAKAKAKEDAAKAKADAAKAKEDASKAKAEAGKAKTDAATAKADSTKAS
                     AASTKEAAPTKAEKAAADKAAKAKAKEEKAAADKAKKEAAAEAKAKKDAEAKAKKDAE
                     AKAKKDAAAAKADEKAKK"
     gene            complement(3940..4947)
                     /gene="rfaD"
                     /locus_tag="VHT22_08690"
     CDS             complement(3940..4947)
                     /gene="rfaD"
                     /locus_tag="VHT22_08690"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEX3137674.1"
                     /translation="MYIIVTGAAGFIGSNLIKALNARGESQIIAVDNLQQADKFRNLV
                     DCEIAEYFDQDEFPTRLVDGDFDDDIAAVLHQGACSDTMQTDGRYMLANNYRYSMVLL
                     RHCQDNDIPFIYASSAAVYGAGREFREESAAEAPLNVYGYSKLLFDQHVRRTLPERTA
                     QIAGFRYFNVYGPREAHKGRMASVVWHFFNQYRATGKVQLFEGSGGYAAGEQRRDFIS
                     VDDVVKVNLDFLDHPQRSGIFNVGTGRAASFNAVAAATINASRADDGAPALSFDELHR
                     TGAIEYIAFPAGLASRYQSFTQADLGRLRAAGYTAPMAPIEQGVANYVRSLLTAADVN
                     P"
     gene            complement(4958..>5563)
                     /gene="alr"
                     /locus_tag="VHT22_08695"
     CDS             complement(4958..>5563)
                     /gene="alr"
                     /locus_tag="VHT22_08695"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX3137675.1"
                     /translation="EDDDGVHEQLEAFERACTGLSYPRSVANSAGVVKYETVGGDYVR
                     PGIMLYGSSPFPYDTADMLGLKPVMTLRSELIAVQDLKANDSVGYGGGYTAARAHRVG
                     VIACGYGDGYSRHAPNGTPVLVCGRKVRTAGRVSMDLMTVDLSDVPEAGIGSPAVLWG
                     EGLSVDDVAAAASTVGYELLCTVTARVPFVTTEVGSVDVEL"
BASE COUNT          863 a         1857 c         1777 g         1066 t
ORIGIN      
        1 ggcgcttgcg gcggcgaacc tcgccttcgg cgctacgccg gtcggagccg actaccggcg
       61 actgctgata ctgatcgagc tcaagggcgg caacgatgga ctcaatacgc tggttccgtt
      121 cgccgatccg gcttattacg cgttgcgccc gaagctcgcg attgcgcgcg actcggtcat
      181 tcagctttcg gatgcggtcg gcctgcatcc tgccctcgag ccgctgctgc cgctgtggcg
      241 cgctcgtgag ctcgccgtgc ttcagggcgt cggatacccg gagcccaacc tgtcgcattt
      301 tcgctcgatc gagatccggg acaccgcttc gggcagtcac cagtatctgc aggacggctg
      361 gttgacgcgc acgttcgcgc tcgcgccgac gccggcagat ttcgccgccg acggcgtgat
      421 cgtcggctcg cccgatctgg gtccgctctc cggcggcggc acgcgcgcga tcgcgctggc
      481 caataccgag caattccttc gccaggcccg cttcgccacg ggcgaaggac agacgcgcaa
      541 caaggctttg cagcacatcc tgaaggtcga agccgatatc gtccaggcgg cggcgcgtct
      601 caaatccgac tatgcgttca aggccagctt cccgcagggc gcgttcggca acgcgatccg
      661 gaccgcctgc caggtcgtcg ccaatcgacc gggcgtggcg gtcgttcgag tgacgcagac
      721 cgggtacgac acgcacagcg ggcaggcgcc gacacaggcg cgactgctgg gcgagctggc
      781 gggaggcgtc gtcgcgctga aaaatgcgct cggcgaggtc aagcgctggg acgacacgct
      841 ggtgctcacc tacgccgaat tcggccgccg gcccaaagag aatctgtcga acggcaccga
      901 tcacggcacg gccagcgcgc atttcgtgct cggcggccgc gtcgcggggg gtctgtatgg
      961 cgagcagccg tcgctcgagc gcttgtccgg cgatggcaac accggatact cgatcgattt
     1021 tcgcagcgtc tacgcgacgg cgctcgaccg ctggtggaac gtcccatcga ccggcccgct
     1081 cggcggtcga ttcacgcctc tcccgcttct caaaacctag acgccgccgg cggcgtgcgc
     1141 gttggcgtcc caggcggcga tgaactcgcg ccaatggggc gcgccggtcg cggcgagctt
     1201 ttcgcgcgcg aacgatagct cggtcgaata ctgggcggcc gacaaggttc cgcgcagcag
     1261 ctggaagcgc aggtagacga ggtaagtgtt catcacgtcg gtttcgcagt aacagcggat
     1321 ctcatcgatc ttgcccgcct cgaatgccgc gtgtacttcg ctgccgtcca tgccgagctt
     1381 tccgggaaag ccgcaaagtc gcgcgatcgc atccaacccg gcggtcgccc gcggctggta
     1441 catcgacagc acgtccatca gatcgagatg ccgcgtgtga tagcgcccca ggtaactgtt
     1501 gaatttgaac tcgcgatcgt cgtcgcccca atcccagaat ctcgccgcgc tgatgccgtg
     1561 taccagcgca cggtgattca gcaccggcag gtcgaagccg ccgccattcc acgaaacgag
     1621 ctgcggcgta tagcgatcga tgccgtcgaa aaaacgacgg atcagctcgg gctcggcgtc
     1681 gctggcctca ccgagcgacc agacgcggaa agtgctgcct tcgcgcaagc cgcaggcgat
     1741 ggcgaccacg cgctgcaggt gcaagggcag aaaatcaccg ccggtcgccg ctcgccgctg
     1801 ctgcgcggcc caggtggcga cctcggcgtc cgacaaggtg gcaggaaggc gattcagccg
     1861 acgcaatccc gcgatgtcgg gcaccgtctc gatgtcgaag acgagggttg gcgtcattgg
     1921 cgttggcgcg gtgcgcgcgt cgcaaacgga cgccggaaca ccgctctagg cagggaaaac
     1981 cccgctcgat aagtagcggt cgccgcgatc gcaggcgacg aacacgattg tcgcgtgcct
     2041 cacctcctcg gcgatgcgaa gcgcgaccgc gcatgcaccg ccggaggata tgccgacgaa
     2101 tatcccctcc tcgcgcgcga gccgccgcgc cgtggcttcg gcgtccgcct gcgagacgtt
     2161 ttcgatgcga tcgacgcgtt ttcgatcgaa gattttcggc aggtacgctt ccggccactt
     2221 gcgtatgccg ggaatctgcg agccttcatc gggctggcac ccgatgatcg cgatggtcgg
     2281 gttctgctcc ttcagatacc gcgatacacc catgatcgtg ccggtcgttc ccatgctgga
     2341 tacgaaatgc gtgatgcggc cgccggtatc gcgccagatt tccgggccgg ttccctcgta
     2401 gtgcgcgagc ggattgtccg gattggcgaa ctggtcgagg atgatgccct tgccatcgtc
     2461 acgcatgcgc tcggcgacat cgcgagcggt ctccatgccg ccggccttcg gcgtcagcac
     2521 gatttcggcg ccgaatgcgc tcatcgtctg ccgccgctcg agcgacagat gctcgggcat
     2581 caccaggatc atccggaagc cgcgcatcgc ggcggccatc gccagcgcaa taccggtgtt
     2641 gccactggtc gcctcgatca gcgtgtcgcc cggtttgatc gtgccgcgct tctcggcttg
     2701 cgcaaccatc gacagcgccg gacggtcctt gaccgatcct gccggattgt tgccctccag
     2761 cttgaccagc acgacattgc cggacgcctt ggccatgcgc tgcagctcga ccagcggcgt
     2821 gttgccgacc gtttgttcga tggtcttgta cacggacgcc attttacccg agcgaccatg
     2881 ttccgatccc gacgcgcgcg tctgaacggg tgtctcaaaa aggcaaaccc cgcgactgcg
     2941 gggtttgccg gtgtttgctt acggacggaa gaacctgctt tgcccctact tcttcgcctt
     3001 ttcgtcggcc ttcgccgctg cggcgtcctt ctttgccttg gcttcagcgt ccttctttgc
     3061 cttggcttca gcgtcctttt tcgccttggc ttcagccgcg gcttccttct tggccttgtc
     3121 ggccgccgcc ttttcttcct tcgccttcgc cttggcggct ttgtcggccg cggccttttc
     3181 ggccttggtc ggcgcggctt ccttggtcga cgccgcgctg gccttggtcg agtccgcctt
     3241 ggcggtcgcg gcgtctgtct tcgccttgcc tgcttccgcc ttggccttgg aggcgtcttc
     3301 cttcgccttc gctgcgtccg ccttggcctt ggcagcgtct tccttcgcct tggctttcgc
     3361 cgccttgtcc gcctcggcct tttctttctt ggtcattttg gcgtctttgg acttcgcgtc
     3421 cttggctgcc gccgcgtcgg tgcctgacgc ggcaccggcc gctccggcgg ctggaggtat
     3481 tgccgccggc gaggccgtct ttgccgcggg cgcgggtgcc ggcgccggcg cgggtgctgc
     3541 ggcttttgca ggcgcggccg cagcggaagg tgccggcgcc acggctttcg caggcgcggt
     3601 cgcaggcagc ggcgtcgacg ctccgctgac cgagatcagc cctttgatct tgccgtaggt
     3661 gccctctttg atgccgctca ccttcatgac atcttccggc gatttgaacg gcccgttggc
     3721 gttgcgatag tcgatgatcg cctgtgcttt caccggcccg atttcgggca acgcctcgag
     3781 ctcggccaga ttcgccgtgt tgatgttcac cgccgccaag gccagcgccg agaacagcac
     3841 ggtgaccagc agcagcaaga gtcttttcat ggttgtttcc ccttggatgg taatgatttg
     3901 tccggcctat ctccggaccc gtgaaacgcg cgagcgcgac taggggttga cgtccgctgc
     3961 cgtgagcagc gaccggacat agttcgcgac gccctgctcg atcggcgcca tcggcgcggt
     4021 gtagccggcg gcgcgcaggc ggcccaggtc ggcttgcgtg aagctctggt atcggctggc
     4081 cagtcccgcc gggaaggcga tgtactcgat cgctccggtg cgatgcaatt cgtcgaacga
     4141 aagcgccggc gcgccatcgt ccgcgcggga ggcattgatc gtcgcggcgg ccactgcgtt
     4201 gaagctggcg gcgcgtccgg ttccgacgtt gaaaatgccc gagcgctgcg gatggtcgag
     4261 aaagtccagg ttgaccttga ccacatcgtc gacgctgatg aaatcgcggc gctgctcgcc
     4321 ggcagcgtac ccgcccgagc cttcaaacag ctgcaccttg ccggtggcgc gatactggtt
     4381 gaagaaatgc cagacaaccg atgccatcct gcccttgtgc gcctcacgcg gtccgtacac
     4441 gttgaaatag cggaagccgg cgatctgcgc ggttcgttcc ggcagggtcc gccgcacgtg
     4501 ctggtcgaac aacagcttcg aatagccata gacgttgagg ggggcctcgg ccgccgattc
     4561 ctctcgaaac tcgcggccgg cgccatacac cgcggccgac gacgcataga tgaaaggaat
     4621 gtcgttgtcc tggcagtgcc gcagcagcac catcgaatac cgatagttgt tggcgagcat
     4681 gtagcgcccg tcggtctgca tcgtgtccga gcacgcgccc tggtgcaaaa ccgccgcgat
     4741 gtcgtcgtcg aagtcgccgt cgacgagccg ggtcggaaat tcgtcctggt cgaagtattc
     4801 ggcaatctcg cagtcaacca gattgcggaa cttgtccgct tgctgcaggt tgtcgacggc
     4861 gatgatctgg ctctcgccac gcgcgttgag tgccttgatc aggttcgagc cgatgaagcc
     4921 ggcggcgccg gtgacgatga tgtacaaacg gtctccccta aagctctacg tcgacgctgc
     4981 cgacctcggt ggtcacgaac ggtacgcggg cagtcaccgt gcaaagaagt tcatagccga
     5041 cggtgctcgc cgcggcggcg acgtcatcga ccgaaaggcc ttcgccccaa agcaccgcgg
     5101 ggctgccgat tccagcctcg ggcacgtccg acagatcgac ggtcatcagg tccatcgaca
     5161 cgcggcccgc agtgcgcact ttcctgccgc aaacgagcac cggcgtaccg ttcggcgcgt
     5221 ggcgcgaata accgtcaccg tagccgcagg cgatcacccc gacgcggtgc gcgcgcgcgg
     5281 cggtgtagcc tccaccgtag ccgacgctgt cgttggcctt caggtcctgg acggcaatca
     5341 gctcgctgcg cagggtcatc acgggcttca gccccagcat gtccgccgtg tcgtacggaa
     5401 acggcgatga accgtacagc atgatgccgg gacggacata gtcgccgccg accgtctcgt
     5461 atttgacgac cccggcggag ttcgccaccg agcgcggata cgacaggccg gtgcacgcac
     5521 gctcgaacgc ctccagctgc tcgtgcacgc cgtcgtcatc ctc
//
LOCUS       DATANS010000591         1840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_824659, whole genome shotgun sequence.
ACCESSION   DATANS010000591 DATANS010000000
VERSION     DATANS010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824659"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VHT22_08700"
     CDS             complement(<1..1029)
                     /locus_tag="VHT22_08700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3137676.1"
                     /translation="MDTETNPSATPELSTPDYGPGAPAPWKRWARWGAIALVAIVVAY
                     FIFGRSGSDKPKYVTQDVTTGDLTVTVTATGNLEPRNQVDIGSELSGTIRTVTVDVND
                     EVKAGQVLTKLDTTRLEAQVLQAQSSLASAEARVLQSLAGLKEARANLARLQKVRELS
                     NNKLPSQQDMDVAESAVAQGEGEEAAARAAVAQAKANLDAVKTDLSKTDIKSPINGVV
                     LVRSVEPGQTVASSLQAPVLFTLAEDLKKMELHVAVDEADIGSVEVGQDASFTVDAFP
                     NRQFHAKITRVDFASNNTQKSSSGSSSSSGSGSSATSTGVVTYETVLEVDNSDSLLRP
                     GMTATAEIV"
     gene            complement(1033..>1840)
                     /locus_tag="VHT22_08705"
     CDS             complement(1033..>1840)
                     /locus_tag="VHT22_08705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX3137677.1"
                     /translation="DITHWRAAAGLATALDEEQAQSTYQQTRAAIPVLQSNLVQAMNR
                     LSVLTGDPPGSLEATLAERKPIPVAPLEIAAGVPADVLRRRPDIRNAERRLAAQTAEV
                     GVATAALYPSLSLTGSITLQALNASDVPNGFRTDRFGLSLNLPIFRGGALRQNLNAQN
                     ALVVQAVATYEATVLAAYEEVENALTASTNEHLRRDALQLGVESARRASDLALTQYNS
                     GLVDFQTVLNADRQLITLEDSLAVSDGEVTANLVRLYKALGGGWSVFPKE"
BASE COUNT          289 a          593 c          603 g          355 t
ORIGIN      
        1 cacgatttcg gcagtcgcgg tcatgcccgg ccgcaacagc gaatccgaat tgtcgacttc
       61 gagcacggtc tcgtacgtga ctacgccggt tgaagtcgcg ctgctgccgg agccgctgct
      121 gctactgctg ccactgctgc tcttctgcgt gttgttcgag gcgaaatcga ctcgcgtaat
      181 cttcgcgtgg aattggcgat tcggaaacgc gtcgacggtg aacgatgcat cctggccgac
      241 ttcgacggag ccgatgtcgg cctcgtcgac ggcgacgtgc agctccatct tcttgagatc
      301 ttcggcgagc gtgaagagca ccggcgcctg cagcgacgag gccactgtct ggcccggctc
      361 caccgaacgc accagcacga cgccgttgat cggcgacttg atgtcggtct tgctgagatc
      421 cgtcttgacc gcgtcgagat tggcttttgc ctgcgcgacg gcggctcggg ccgcagcctc
      481 ttcgccttcg ccttgcgcga ctgccgactc ggcaacgtcc atgtcctgct gcgagggcag
      541 tttgttgttg ctcagctcgc gcaccttctg gagtcgagca aggttggcgc gcgcttcctt
      601 gagtcccgca aggctctgca ggacgcgcgc ttcggccgac gcaagcgagc tctgtgcctg
      661 gagcacctgc gcttccaggc gcgtggtgtc gagcttggtg aggacttgcc ccgccttcac
      721 ctcatcgttc acgtcgaccg tgacggtacg gatcgtgccc gacagctcgc tgccgatgtc
      781 gacttgattg cgcggttcga gattccctgt ggccgtcacg gtgaccgtga ggtcgccggt
      841 cgtgacgtcc tgcgtcacgt acttgggctt atcgctgccg ctgcgcccga agatgaaata
      901 agcgacgacg atcgcgacca gcgcgatcgc accccagcgg gcccagcgct tccacggcgc
      961 cggcgcgccc gggccgtagt ccggtgtcga gagctcgggg gtcgccgagg gattggtttc
     1021 agtgtccacg atctattcct tcgggaagac gctccagccg ccgcccagcg ccttgtacag
     1081 gcgcacgagg ttcgccgtca cttcgccgtc gctgacggcg agcgagtcct cgagcgtgat
     1141 gagctgacgg tcggcgttga gcacggtctg gaaatccacg agtccggagt tgtattgcgt
     1201 gagtgcgaga tcggacgcgc gccgggcgct ctcgacgccg agctgcaagg cgtcgcgtcg
     1261 cagatgctcg ttggtcgagg cagtgagcgc attctcgact tcctcgtagg cggcgagaac
     1321 agtcgcttca tacgtcgcga ccgcttggac gacgagcgca ttctgcgcat tcaagttctg
     1381 gcgcagcgcc ccgccgcgga agatcggcag attcaacgac aggccgaagc gatcggtgcg
     1441 gaagccattc ggcacgtcgc tcgcattcag cgcttgcaac gtgatcgagc cggtcaacga
     1501 cagactcgga tagagcgccg cggtcgcgac accgacttcg gcagtctgcg ccgcgaggcg
     1561 ccgctcggcg ttgcgaatgt caggacgacg acgcaagaca tcggccggca cgccggcagc
     1621 gatctcgagc ggtgcgaccg gaatcggttt gcgctccgcg agggtcgctt cgagcgaacc
     1681 gggcggatcg cccgtcagca ccgacaagcg attcatcgcc tgcacgaggt tcgattgcag
     1741 caccgggatc gctgcacgcg tttgctggta cgtgctctgc gcctgctctt catcgagcgc
     1801 cgtcgccagg cccgcagccg ctcgccagtg agtgatatcg
//
LOCUS       DATANS010000592         1508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_113131, whole genome shotgun sequence.
ACCESSION   DATANS010000592 DATANS010000000
VERSION     DATANS010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113131"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="VHT22_08710"
     CDS             complement(<1..859)
                     /locus_tag="VHT22_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX3137678.1"
                     /translation="MSALDKIDLLEHGVLRFITAGSVDDGKSTLIGRLLYDTKTILAD
                     QLAAIARTSQRRGGPLDLSLLTDGLSAEREQGITIDVAYRYFATALRKFIIADTPGHE
                     QYTRNMVTGASTADLAILVVDARHGVVTQTRRHATIAHLVGIPHLVVAVNKMDLVDFD
                     QRVFAAIVADFTAFAQASGIRDARFVPMSALDGDMVVDRGNRLPWYTGPTLLQILETV
                     ESAPTLAQGPLRFPVQFVARPAAGQPRGYMGRIESGAIAVGDVVTVLPSLQRARVRAI
                     RTYEGDRRHA"
     gene            complement(859..>1508)
                     /gene="cysD"
                     /locus_tag="VHT22_08715"
     CDS             complement(859..>1508)
                     /gene="cysD"
                     /locus_tag="VHT22_08715"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX3137679.1"
                     /translation="LIVRSVDDSIAKGRVGLANPDESRNRHQSVTLLDAIAELGFDAC
                     IGGARRDEEKARAKERVFSFRDAFGQWDPKNQRPELWNLYNARVGPGEHIRVFPISNW
                     TELDVWQYIARERLEVPSLYLAHRRRVVRRGTALVPVTALTPPREGETVEHISVRFRT
                     VGDISCTAPVASDAATIEAIIAETAVATITERGATRLDDQTNESSMELRKKEGYF"
BASE COUNT          247 a          481 c          511 g          269 t
ORIGIN      
        1 cggcatgccg ccgatcgcct tcgtacgtcc ggatggcgcg cacgcgggcg cgttgcagcg
       61 acggcagcac cgtcacgaca tcgccgacgg cgatcgcgcc cgattcaatc cggcccatgt
      121 agccgcgcgg ttgccctgca gccggcctcg cgacgaattg caccgggaag cgcaacggtc
      181 cttgagcaag cgtcggcgcc gactccaccg tttcgagtat ctgcagcagg gttggtccgg
      241 tgtaccaggg gaggcgattg ccgcggtcga cgaccatgtc gccgtcgagc gccgacatcg
      301 gaacgaatcg cgcatcgcgt atgcccgagg cctgggcaaa ggcagtgaag tccgcgacga
      361 tcgcggcaaa gacgcgctgg tcgaagtcga ccaggtccat cttgttgacc gcgaccacga
      421 ggtgcggaat gccaaccaga tgagcgatcg tcgcatgccg ccgcgtctgc gtaacgacgc
      481 cgtgccgtgc gtcgacgacc aggatcgcca ggtcggcggt gctggcgccg gtcaccatgt
      541 tgcgcgtgta ttgctcgtgg ccgggcgtgt ccgcgatgat gaatttgcgc agcgccgtcg
      601 cgaaataccg gtaggcgacg tcgatcgtga tgccttgctc gcgctcggcg ctgagaccgt
      661 ctgtcagcag tgacagatcg agcggcccgc cgcggcgctg cgaggtgcgg gcgatcgcag
      721 ccagctggtc ggccaggatg gtcttggtgt cgtagagcag gcggccgatc agcgtgctct
      781 tgccgtcgtc gacgcttccc gccgtgataa agcgcagtac accgtgctcg agcaggtcga
      841 ttttgtccag cgcagacatc agaaatagcc ttccttcttg cgcaattcca tcgatgattc
      901 gttggtctgg tcgtcgagtc gggtcgcacc gcgctcggtg atcgtcgcca ccgcggtctc
      961 ggcgatgatc gcttcgatcg tcgccgcgtc ggaagcgacc ggcgccgtgc agctgatatc
     1021 gccgacggtg cgaaagcgca ccgaaatgtg ctcgacggtc tcgccctcgc gcggtggcgt
     1081 cagggcggtc accggcacca gcgccgtgcc gcggcgaacc acgcgccgcc gatgcgccag
     1141 gtagagcgac ggcacttcga ggcgttctcg cgcgatgtat tgccagacat cgagctcggt
     1201 ccagttgctg atcgggaaaa cgcggatgtg ctcgccgggc ccgacgcgcg cgttgtacag
     1261 gttccataac tcgggacgct ggttcttcgg gtcccattgt ccgaacgcat cgcgaaacga
     1321 gaacacgcgt tccttggcgc gagccttttc ctcatcccgg cgcgcgccgc cgatgcaggc
     1381 gtcgaagccg agttcggcga tcgcgtcgag cagggtcacc gattgatggc ggttgcggct
     1441 ttcatcggga ttggcgagac cgacacggcc tttggcgatc gagtcgtcga ccgagcgtac
     1501 gatcaaac
//
LOCUS       DATANS010000593         1982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_659764, whole genome shotgun sequence.
ACCESSION   DATANS010000593 DATANS010000000
VERSION     DATANS010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659764"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..451)
                     /locus_tag="VHT22_08720"
     CDS             complement(107..451)
                     /locus_tag="VHT22_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137680.1"
                     /translation="MNKFVAVFFAAALALALNACGDNGKAAADAKAASDKAAAAAKEA
                     SDKAAAAAKDAGTAAANAGAAANDAAKAAGGTAAMDSSKAPAGTMAPAATPPASTMAP
                     APDQSKSDATKK"
     gene            complement(654..1166)
                     /locus_tag="VHT22_08725"
     CDS             complement(654..1166)
                     /locus_tag="VHT22_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137681.1"
                     /translation="MTDPTPAEAPAEPRLIELSTLAEQTEAIDTLIELARQHIRVFDQ
                     DLSQTGWGAAGRADRLAAFLRQVRGRRLDIIVHDTRYLQSACPHLLSLLRNYGHALTI
                     YQTGPEGKVATDPLVIVDGRHYLHRFHFEQPRASLGIDQPEQTQPLASRFDEIWATGE
                     AGLSGTVLGL"
     gene            complement(1178..>1982)
                     /locus_tag="VHT22_08730"
     CDS             complement(1178..>1982)
                     /locus_tag="VHT22_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3137682.1"
                     /translation="FIPYARLDDLMISYAAPGGGVGAHADSYDVFLLQGEGRRRWRIS
                     RQRDLSLKPASPLKLLAKFHPDSEWTLGPGDMLYLPPDHAHEGVAIDACTTYSIGFRA
                     ASTQQLATAFLDWLRDEVALDGRYADPDLRPSRAPARIGAAMQAQCADALGRIRWSRS
                     ATDVFLGCYLTEPKQNVYFTPPRRPLTPRAFAAAAARRGVRLDARTRLLYDGRCIFIN
                     GAAIARPGTGGPAIELLANARAMKSGKPAMAAPAELLYRWYCDGYLHLD"
BASE COUNT          345 a          643 c          646 g          348 t
ORIGIN      
        1 ccgaatcggc gccccgctct gacccttccg ccacgctgca aggcggtgac ctgcggatca
       61 aaaaaaagcc ggcgcaagcc ggcttttcct tgctgctgaa cttgaattat ttcttggtgg
      121 cgtcggactt ggactgatcc ggtgccggcg ccatcgtgct ggcgggcggc gtcgccgcgg
      181 gcgccatcgt gccggccgga gccttgctcg aatccatcgc agcggtacca cccgcggcct
      241 tggctgcatc gttcgccgca gcgcctgcgt tcgcagcggc agtgcccgcg tccttggccg
      301 cggccgcagc cttgtcgctc gcttccttgg cggcggcagc cgccttgtcg gatgctgcct
      361 tggcgtcagc cgccgccttg ccgttgtcgc cgcaagcgtt cagcgcaagc gccaacgccg
      421 cggcgaagaa taccgctaca aacttattca tggggttccc ctacttgtgt atgaaggatg
      481 gaaattcaac agtcgaatat tcggtggcga aaatcggctt ccgcagacgg ccgattcaag
      541 cgtttcggca gggcagcgga ggtgcaaccg acttcgaaat gttagcacaa agacgatgga
      601 tttacatgtc tcgaagcatg caaggtgcgc ctttgcgccc gcgcggtccg cgctcaaagg
      661 cccagcacgg tgccgctcaa gcccgcttcg ccggtcgccc agatctcgtc gaagcggctg
      721 gccagcggct gcgtctgctc cggctgatcg atgccgagcg aggcgcgcgg ctgctcgaag
      781 tgaaagcgat gcagataatg cctgccatcg acgatcacca gaggatcggt cgctacctta
      841 ccctccggtc cggtctgata gatcgtcagc gcgtggccgt agttccgcag caggctcagc
      901 aagtgcggac aagcgctctg cagatatcgt gtgtcatgca cgatgatatc cagtcgccgg
      961 ccgcggacct ggcgcaggaa cgcagccaga cgatcggcgc ggcccgcggc accccacccg
     1021 gtttgcgaca ggtcctgatc gaagacacgg atatgctgcc gcgccagctc gatcaaagtg
     1081 tcgatggctt cggtctgctc cgccagcgtt gaaagctcga tcaggcgggg ctcggcggga
     1141 gcctcagccg gcgttggatc ggtcatggcc gcttgcttta atcaaggtgg agatagccat
     1201 cgcaatacca tcgatagagc agttcggccg gcgcggccat cgccggtttg ccgcttttca
     1261 tggcgcgggc attggccaaa agctcgatcg ccggtccgcc tgttccaggc cgcgcgatcg
     1321 cagcaccatt gataaagatg cagcgaccat cgtatagcaa ccgcgtgcgc gcgtccaaac
     1381 gcacgccgcg tcgcgccgct gccgcggcga acgcgcgcgg cgtcaaagga cgccgcggcg
     1441 gcgtgaaata gacgttctgt ttcggctcgg tcagatagca gccgagaaag acgtcagtcg
     1501 cagaacggct ccagcggatg cggccaagcg cgtccgcgca ttgcgcttgc attgccgcgc
     1561 cgatgcgcgc cggcgcgcgc gacggccgca aatccggatc ggcgtaacgg ccgtcgagtg
     1621 cgacttcgtc gcgcagccag tcgagaaagg cagtcgcgag ttgctgcgtc gaagctgcgc
     1681 ggaacccgat cgaataggtc gtgcaggcgt cgatggcaac gccctcgtgc gcatggtcgg
     1741 gcggcaggta cagcatgtcg cccgggccca gcgtccactc gctgtcggga tggaacttcg
     1801 cgagcaactt gagcggcgat gccggcttga gcgacagatc gcgctggcgg ctgatgcgcc
     1861 agcgtcgccg cccctcgccc tgcagcagga agacgtcata cgaatccgcg tgcgcgccca
     1921 cgcctccgcc cggcgcggcg tagctgatca tcaggtcgtc gagtctcgcg tacgggatga
     1981 ag
//
LOCUS       DATANS010000594         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_615255, whole genome shotgun sequence.
ACCESSION   DATANS010000594 DATANS010000000
VERSION     DATANS010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615255"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /gene="secF"
                     /locus_tag="VHT22_08735"
     CDS             <1..798
                     /gene="secF"
                     /locus_tag="VHT22_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX3137683.1"
                     /translation="VIEIAYNHPADLSRVRGVIDKMNLGEFAVQSFGSSNSALIRLPL
                     KEGVSTAQLSERVMAALRADDAAVTQQRVEFVGPQVGKELYENGALALLLVSFGIVAY
                     LALRFEWRFAVSAIIANLHDVIIILGFFALFQWEFSLPVLAAVLAVLGYSVNESVVIA
                     DRIRENFRKMRKASVPQVINSAITSTLSRTIITHGCTLMMVLSIFFFGGETLHHFALA
                     LAIGICFGIYSSALVMASLVMWLGVSREDLVKPDKKSGAAGAEKILP"
     gene            829..1479
                     /locus_tag="VHT22_08740"
     CDS             829..1479
                     /locus_tag="VHT22_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX3137684.1"
                     /translation="MLTTLLYSLLALDSTLAELAAQHSWVLFVALFLVIFAETGLVVF
                     PLLPGDSLLFLAGTVAAASGIDVHLLAIVLFIAAVTGDSVNYAVGHFLGPKVFNLPPD
                     SKLSRWLKPEYLERTHRFYEKYGGFTIIICRFVPIVRTFAPFLAGVGAMTYRRFLAYN
                     VIGAGVWVAALVYAGYLFGNIPWVKENLTFIVLAVVGISLLPVVVQWLRERRRSDG"
     gene            complement(1519..>2260)
                     /locus_tag="VHT22_08745"
     CDS             complement(1519..>2260)
                     /locus_tag="VHT22_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX3137685.1"
                     /translation="VGNYNAHLAAYPDVDWEALSRRVVSQLGMEFNAYTTQIEPHDGL
                     AAFFDALARANTILIDFNRDIWGYVSLGYFRQGVSEGEVGSSTMPHKVNPIDFENSEG
                     NLGLANALLHHLAQKLPISRWQRDLTDSTVMRNVGVAFGYALLGYVSCRQGLGRLAAD
                     PARLAADLDANWEVLAEPIQTVMRRYGIDNPYEQLKALTRGKAGMTRETLHAFIDALA
                     LPPEAKTRLKALTPSSYIGKASELAKRV"
BASE COUNT          355 a          723 c          714 g          468 t
ORIGIN      
        1 gtgatcgaga tcgcgtacaa ccatcccgcc gatctctcgc gtgtccgcgg tgtgatcgac
       61 aagatgaatc tgggcgagtt cgcggtgcag agcttcggca gctccaacag cgccttgatc
      121 cggctgccgt tgaaggaagg cgtgtcgacc gctcagctct ccgagcgggt gatggcggcg
      181 ctgcgggcgg acgatgccgc agtcacccag cagcgcgtcg agttcgtcgg gccgcaggtc
      241 ggcaaggagc tctacgaaaa cggcgcgctg gcgctgctgc tggtgagctt cggcatcgtg
      301 gcttacctgg cgctgcgttt cgaatggcgc ttcgccgtgt cggcgattat cgccaatctg
      361 cacgacgtca tcatcattct cggctttttc gcgctattcc aatgggagtt ctcgctgccc
      421 gtgctggccg cggtgctggc ggtgctcggc tactcggtca acgaatcggt cgtcatcgcc
      481 gatcggattc gcgagaactt ccgcaagatg cgcaaggcca gcgtgccgca ggtcatcaac
      541 agcgcgatca ccagcacgct ttcgcgcacg atcatcacgc atggttgtac cttgatgatg
      601 gttctgtcga tcttcttctt cggcggcgag acgctgcacc atttcgcatt ggcgctcgcc
      661 atcggcatct gcttcggcat ctactcgtcg gcgctggtga tggcctcgct ggtgatgtgg
      721 ctgggggtgt cgcgcgagga cctggtcaag ccggacaaga aaagcggcgc ggcgggtgcc
      781 gagaagatcc tgccctgaca ggtcggcaat cgctgccggg caggcgcgat gctgaccacg
      841 ctgctgtact cgctgctggc gctggactcg acgctcgccg agcttgccgc gcagcactcg
      901 tgggtactgt tcgtagcgct gttcctggtg atttttgccg agaccggcct ggtcgttttt
      961 ccgctactgc ccggcgactc gctgcttttc ctggccggca ccgtcgccgc ggcaagcggc
     1021 atcgacgtgc atctgcttgc gatcgtgctc ttcatcgcgg cggttaccgg cgattcggtc
     1081 aattacgccg tcgggcactt cctcgggccg aaggtattca atctgcctcc tgacagcaaa
     1141 ctctcacgct ggctgaagcc cgaatacctg gagcgcacgc accgcttcta cgaaaaatac
     1201 ggcggattca cgatcatcat ctgccgcttc gttcccatcg tgcgaacgtt tgcgccattt
     1261 ctcgccggcg tcggcgcgat gacttaccgc cgcttcctcg cctacaacgt gattggcgcc
     1321 ggcgtatggg tcgcggccct ggtgtacgcc ggctatctgt tcggcaatat cccgtgggtc
     1381 aaggagaacc tgaccttcat cgtgctggct gtcgtcggca tttcgctgct gccggtcgtc
     1441 gtgcagtggc tgcgcgaacg gcggcgaagc gacggttagg cggaccggat ctcgcgcgcg
     1501 caccctcctg gcgttccgct agacccgttt ggcgagctcg ctcgctttgc cgatgtagct
     1561 cgacggcgtc agcgccttca atcgcgtctt ggcttccggt ggcagcgcca gcgcgtcgat
     1621 gaacgcatgc agcgtctcgc gcgtcatccc cgccttgccc cgggtcagag ccttcagctg
     1681 ctcgtacgga ttgtcgatgc cgtagcgccg catcaccgtc tggatcggtt ccgcgagcac
     1741 ttcccagttt gcgtcgaggt cggcggcgag ccgcgccgga tcggccgcga gacggcccag
     1801 tccctgccgg caggagacgt agccgagcag cgcgtagccg aaagcgaccc cgacattgcg
     1861 catgaccgtc gagtcggtca ggtcccgctg ccagcgcgag atcggcagct tttgagcgag
     1921 gtggtgcagc aacgcgttgg ccagaccgag attgccctcc gagttctcga agtcgatcgg
     1981 gttgaccttg tgcggcatcg tcgacgagcc gacctcgcct tcgctgactc cctggcgaaa
     2041 atagccgagc gacacgtaac cccagatgtc gcgattgaaa tcgatcagga tggtgttggc
     2101 tcgcgccagc gcatcgaaga acgcagccag cccgtcatga ggctcgatct gcgtcgtgta
     2161 cgcattgaac tccatgccga gctgcgagac gacgcgccgc gacagcgctt cccaatccac
     2221 atccggatac gcggccagat gcgcgttgta gttaccgacg
//
LOCUS       DATANS010000595         2815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_29298, whole genome shotgun sequence.
ACCESSION   DATANS010000595 DATANS010000000
VERSION     DATANS010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29298"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VHT22_08750"
     CDS             complement(<1..215)
                     /locus_tag="VHT22_08750"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX3137686.1"
                     /translation="MLVHPQFDPVAIHLGPLAVRWYGLMYLVGFVLFVVLGKIRARQN
                     LLTGWHPRDVDDMLFYGVFGVIIGGRLG"
     gene            complement(244..1152)
                     /locus_tag="VHT22_08755"
     CDS             complement(244..1152)
                     /locus_tag="VHT22_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX3137687.1"
                     /translation="MLDLRRLRYFVAVAEALHFGRAAARLHMSQPPLSRQIQQLEREI
                     GVLLFRRSKRRVELTDAGAYLLDQAREMLADAETLAVRTRRVESGETGRLTLGFISTV
                     DYSILPGLLSAYRAAHPGVTLELRELTSDVQLRELHEGRIDAGMLLAPVDDTALAMLP
                     LLREPLIAALPADDPLARSHAALSLASLAERPFVIFPRPAAAGLYDAIIDFCRRCDFT
                     PRIAQEAIQMQTIVSLVSAGLGVALVPASLRHMRRRGVVYRRLREDSPLLAVLLAWRT
                     ANRSACLSQFVATARKSVRGARRGAG"
     gene            1242..2156
                     /locus_tag="VHT22_08760"
     CDS             1242..2156
                     /locus_tag="VHT22_08760"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX3137688.1"
                     /translation="MRTSILDRYHPATLLSLTSLFWAGNMVIGRAVADAMSPIGLSYW
                     RWVIVIAVLAPFCWREVLAKRTQLSRSWRILLLLSVMSTGLYNALTYWSLHYTTATNT
                     TLLNSTIPIWVMVASWILLRHLPPTRALLGFLTSLLGVLFIVAHGHPGALFTLAPNVG
                     DLIMLIALFIWGFYAVLLRYRPADLSPLGYVFVTGVIGTLIATPFFLIDRAVSATPDL
                     SWQALAAIGYFALFSSLAATLCYNNAIDRVGPVHASLFIHLVPLFGSLLAIAFLGERP
                     GWQHLIGMALILSGILQARRAAVAAPRR"
     gene            2232..>2815
                     /locus_tag="VHT22_08765"
     CDS             2232..>2815
                     /locus_tag="VHT22_08765"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011694362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX3137689.1"
                     /translation="MANNRRSWIITQGVARSPNRAMLRAVGFGDGDFDKPIVGVANGH
                     STMNPCNAGIQPLVDRAMAALAAAGAKPQVFGFPTVTDGIGMGTEGMKYSLVSREVIA
                     DSIEVAVMGQCMDGVVCVGGCDKNMPGSMMAMARMNVPAIFVYAGTIKPGFWKGEKLT
                     IVSPFEAVGAFTAGKMSKEDLDGIEKNACPTVGAG"
BASE COUNT          492 a          935 c          909 g          479 t
ORIGIN      
        1 ccgagccgac cgccgatgat cacgccgaac acgccataga acagcatgtc gtcgacgtcg
       61 cgcggatgcc agccggtgag gagattttgc cgcgcccgga tcttgcccag gaccacgaac
      121 aacacgaatc cgaccaggta catgagcccg taccagcgca ccgcaagggg gcccagatgg
      181 atggcgaccg gatcgaattg cgggtggacc agcatcgggc gaattatgac attgccgccg
      241 cgcctacccg gcgccccgcc gcgctccgcg gaccgacttg cgcgccgtgg ctacgaattg
      301 cgagaggcat gcgctgcggt tggccgtccg ccacgcgagc agcaccgcca gcagcggcga
      361 atcctctcgc aaccggcggt agacgacgcc gcggcgccgc atgtggcgca gcgacgccgg
      421 caccagggca acgccaaggc cggcggatac aagactgacg atcgtctgca tctggatcgc
      481 ttcctgcgcg atgcgcggcg tgaagtcgca ccgccggcag aaatcgatga tcgcgtcgta
      541 caggcctgct gcggcaggac ggggaaagat gacgaacggc ctctccgcca gcgaggccag
      601 cgaaagcgcc gcgtgcgatc gcgccagagg atcgtctgcc ggcaaggcgg caatcagcgg
      661 ctcgcgcagc agcggcagca tcgcgagtgc agtatcgtcc accggcgcca gcagcatgcc
      721 cgcgtcgatg cgcccctcgt gcagctcgcg caactggacg tcgctggtca gctcgcgcaa
      781 ctcgagcgtc acgccaggat gcgctgcgcg ataggcgctg agcagtcccg gcaggatgct
      841 gtaatcgacg gtgctgatga aaccgagcgt caatcgcccg gtctccccgg actcgacccg
      901 ccgcgtccgg acggcgagcg tctcggcatc ggcaagcatt tcgcgcgcct gatccagcag
      961 ataggccccg gcgtcggtca actcgacccg acgcttgctg cggcgaaaca gcagcacgcc
     1021 tatctcacgc tcgagctgct ggatctgacg cgacagcggc ggctgcgaca tgtgcaggcg
     1081 cgccgcggcg cgcccgaagt gcagtgcctc ggcgacggcc acgaagtagc gcaggcggcg
     1141 gagatccagc attgatacct ctatagcatc aattatagcg ataaagtgta ttggacagta
     1201 ttagagcccg cgcgctataa aagccgtcaa gggccttacc gatgcgcacc tcgatcctcg
     1261 accgctatca cccggcgacg ttgctgtcgc tgacctcgct gttctgggcc ggtaacatgg
     1321 tcatcggccg cgccgtcgcc gacgcgatgt cgccgatagg cctgtcgtac tggcggtggg
     1381 tgatcgtgat cgccgtgctg gcgccgttct gttggcggga agtgctcgcc aagcgaacgc
     1441 agctgtctcg atcgtggcgg attctgctgc tgctgagcgt aatgagcacc gggctttaca
     1501 acgcgctgac ctactggtca ctgcactaca cgacggcgac caacacgaca ctgctgaact
     1561 cgacgatccc gatctgggtg atggtcgcgt cgtggattct gctcaggcac ttgccgccga
     1621 cgcgcgcgct gctcggcttc ctgacttcac tgctcggcgt gctgttcatc gtcgcgcatg
     1681 gccatccggg cgcgctattc accctggcgc cgaacgtcgg cgacctgatc atgctgatcg
     1741 cgctgttcat ctggggcttt tacgcggtgc tgctccgcta tcgcccggcc gatctttcgc
     1801 cgcttggtta tgtgttcgtc accggcgtga tcggcacgct gatcgcaacg ccgttcttcc
     1861 tgatcgatcg cgccgtgtcg gcaacgcccg acctgtcgtg gcaggcactg gcggcgatcg
     1921 ggtatttcgc actgttctcc agtctcgccg cgacgctttg ctacaacaac gcgatcgatc
     1981 gggtcggccc cgtccacgca agtctgttca tccacctcgt gccgctgttc ggaagcctgc
     2041 tcgcgatcgc ctttctcggc gaacgcccgg gttggcagca tctgatcggc atggccctca
     2101 ttttgtccgg gatactgcag gcccggcgcg ccgcggtcgc cgcaccaagg cgctgacgca
     2161 tccgcaccgc actcgcgcga agcgacggcg gcaccgtaca tcgacacccc tatcaggatt
     2221 ccccgagagc catggccaac aaccgacgca gctggatcat cacccaggga gtcgcccgct
     2281 cgcctaaccg cgcgatgctg cgcgcggtag gtttcggcga cggcgatttc gacaagccta
     2341 tcgtcggcgt ggccaacggc cacagcacga tgaatccctg taatgccggg atccagccgc
     2401 tggtcgaccg cgcgatggcg gcgctggccg cagccggtgc gaagccgcag gtcttcgggt
     2461 tcccgacggt gaccgacgga atcggcatgg gcaccgaagg catgaaatat tcgctggtgt
     2521 cgcgcgaggt catcgccgac tcgatcgagg tcgccgtgat ggggcagtgc atggacggcg
     2581 tcgtttgcgt cggcggctgc gacaagaaca tgccgggctc gatgatggcg atggcgcgga
     2641 tgaacgtgcc cgcgatcttc gtctacgccg gcacgatcaa gcccggtttc tggaagggcg
     2701 agaagctcac catcgtcagc ccgttcgagg cggtcggcgc gttcaccgcc ggtaagatgt
     2761 cgaaggagga tctggacggc atcgaaaaaa acgcctgtcc gaccgtcggg gccgg
//
LOCUS       DATANS010000596         2781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_366682, whole genome shotgun sequence.
ACCESSION   DATANS010000596 DATANS010000000
VERSION     DATANS010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366682"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VHT22_08770"
     CDS             <1..254
                     /locus_tag="VHT22_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HEX3137690.1"
                     /translation="TTVEEVNAVMKAASESEPLHGLLGYNTAPLVSIDFNHDPRSSIF
                     DATLTKVSGRLVKVSSWYDNEWGFSNRMLDATVALMAAK"
     gene            306..1505
                     /locus_tag="VHT22_08775"
     CDS             306..1505
                     /locus_tag="VHT22_08775"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX3137691.1"
                     /translation="MSDMAATFLRLADVDVAGKRVFIRADLNVPLDGQGAISDDTRIR
                     ASVPAIRDALSRGGATMVTSHLGRPSEGRFSAADSLAPIAQRLGELLGRPVPLIADWV
                     DGGAWHEALKPGDLVLLENCRFNVGEKKDDETLSRKMAKLCDVYVNDAFGTAHRAEAT
                     THGIARFAPIACAGPLLAAEIDALGRALAHPARPLVAIVAGSKVSTKLTILKSLAARV
                     DRLIVGGGIANTFILAVGGRIGKSLAEADLANEAKAILDAFPGKVPIPVDAVVAKEFS
                     ATAAPALKAIADVAADDMILDIGPRTVAALKAIIAKAGTIVWNGPVGVFEFDAFGDGT
                     RELAHAIADSNAFSIAGGGDTLAAIAKYGVADRIGYISTGGGAFLEFLEGKTLPAVAI
                     LEQRASR"
     gene            1543..>2781
                     /gene="pyk"
                     /locus_tag="VHT22_08780"
     CDS             1543..>2781
                     /gene="pyk"
                     /locus_tag="VHT22_08780"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX3137692.1"
                     /translation="MQRSTKIVATLGPASSSADVLTRMLAAGVDVVRLNFSHGTAADH
                     QERARMVRDSARSLGREVAIMADLQGPKIRIGKFAAGKVVLKTGQQFTLDAARDLGDE
                     EGVGLDYKELTHDVAPGVILLLNDGLIRLRVESVRGTAIVTRVEVGGALSNNKGINRM
                     GGGLTAPALTAKDMDDMKTAVAIDAEYLAVSFPKNKEDMYMARQLLRAAGGRAFLIAK
                     IERAEAINALEEIIDASDGIMVARGDLAVEVGNATVPGLQKRMIKLARERNKLTITAT
                     QMMESMIENPVPTRAEVSDVANAVLDGTDAVMLSAETAAGRFPVETIESMSQICAEAE
                     KTESSELDRDFLDRTFTRIDQSIAMAALFTAYHLKVKAIAALTQSGSTALWMSRHNCG
                     CPIFALTPEVSSQRRMALYRN"
BASE COUNT          492 a          901 c          925 g          463 t
ORIGIN      
        1 atacgacggt cgaggaggtc aatgcagtga tgaaggcggc atccgagtcc gaaccgctgc
       61 atggattgct cggctacaac accgcgccgc tggtatcgat cgacttcaac catgacccgc
      121 gctcgtcgat cttcgatgcg acgctgacca aggtctccgg acgcctggtc aaggtttcgt
      181 cctggtacga caacgaatgg ggcttcagca accggatgct cgatgccacg gtggcgctga
      241 tggcggccaa atagcaccgc tgcgaaagtc gcgcgatcgt ttgcggcaac tgctgacgat
      301 ggcgcatgtc tgatatggcc gcaacgttcc tgcgcctggc cgacgtcgac gtcgccggca
      361 agcgcgtctt catccgcgcc gatctgaacg tgccgctcga cgggcaaggc gcgatcagcg
      421 atgacactcg catccgtgcc tcggtgccag cgatccgcga cgcgctgtcg cgcggtggcg
      481 cgacgatggt tacctcgcat ctgggtcgac cgagcgaagg tcgattcagc gccgccgatt
      541 cgctggcgcc gattgcccag cgactcggcg agctgctcgg gcggccggtg ccgttgatcg
      601 cagactgggt cgacggcggc gcctggcatg aggcgctcaa gccgggcgat ctggtcctgc
      661 tcgagaactg tcgcttcaac gtcggcgaaa aaaaggacga cgagacgctg tcgcgcaaga
      721 tggcgaagct gtgcgatgtc tacgtcaacg acgccttcgg aaccgcgcat cgcgccgagg
      781 cgacgacgca cggcatcgcc cggtttgcgc cgatcgcctg tgccggtccg ctgctcgccg
      841 ccgagatcga tgcgctgggg cgcgcactcg cgcatccagc ccggccactg gtcgcaattg
      901 tcgccggctc gaaggtgtcg accaagctga cgatactgaa gtcgctggcg gcgcgggtcg
      961 atcgtctgat cgtcggcggc ggcatcgcca atacgttcat cctcgcggtc ggcggccgga
     1021 tcggcaagtc gctcgcggag gccgatctcg ccaatgaggc gaaggcgatt ctcgacgcgt
     1081 ttccgggcaa ggtgccgatt cccgtcgacg ccgtcgtcgc caaggagttt tcggcgaccg
     1141 cggcgcctgc gttgaaggcc atcgccgacg tcgccgccga cgacatgatc ctcgacatcg
     1201 ggccgcggac ggtggcggcg ctgaaggcga tcatcgccaa ggccggcacg atcgtctgga
     1261 acggacctgt cggcgtattc gagttcgatg ccttcggtga cgggacgcgc gagctcgccc
     1321 atgcgatcgc cgattcgaac gcattttcga tcgccggcgg cggcgacacg ctggccgcga
     1381 tcgcgaagta tggcgtcgcc gatcgcatcg gctacatctc gaccggcggt ggcgcgtttc
     1441 tcgaatttct cgaaggcaag acgcttcctg ccgtcgcgat cctcgagcag cgcgcctcgc
     1501 gatgaagcgc gggacaacga tccttttcac gcgagaccct cgatgcaacg ctcgaccaaa
     1561 atagtcgcca cgctcggtcc ggccagcagc tccgccgacg tgctgacgcg aatgctcgct
     1621 gccggcgtcg atgtcgtacg gctgaacttt tcgcacggca ccgcggcgga ccaccaggag
     1681 cgcgcgcgca tggtgcgcga ttcggcgcga tcgctgggcc gcgaggtcgc aatcatggcc
     1741 gacctgcagg gcccgaagat ccgcatcggc aagttcgccg ccggcaaggt cgtgctcaag
     1801 acaggccagc agttcacgct ggacgccgcg cgggacctgg gcgacgagga aggcgtcggc
     1861 ctcgactaca aggagctgac ccacgatgtc gcgcccggcg tcatcctgct gctcaacgac
     1921 gggctgatcc gccttcgtgt cgagtcggtt cgcggcaccg cgatcgtcac gcgcgtcgag
     1981 gtcggcggcg ctctgtcgaa caacaaaggc atcaaccgca tgggcggcgg cctgacggcg
     2041 cccgcgctga cggccaagga catggacgac atgaaaaccg cggtcgcgat cgacgcggaa
     2101 tacctggccg tgtccttccc gaagaacaag gaagacatgt acatggcgcg ccagctgctg
     2161 cgcgccgccg gcgggagggc attcctgatc gccaagatcg agcgggccga ggcgatcaac
     2221 gcgctggagg agatcatcga tgcgtccgac ggcatcatgg ttgcccgcgg cgatctcgcg
     2281 gtcgaagtgg gcaacgcgac cgttcccggc ctgcagaagc ggatgatcaa gctcgcccgc
     2341 gagcgcaaca agctgacgat caccgcgacg cagatgatgg agtcgatgat cgagaatccg
     2401 gtgccgaccc gcgccgaggt gtccgacgtc gccaatgcag tcctcgacgg gaccgacgcg
     2461 gtcatgcttt cggccgagac cgccgccggc cggtttccgg tcgagacgat cgagtcgatg
     2521 agccagatct gcgccgaggc ggagaagacc gagtcgagcg agctcgatcg cgatttcctc
     2581 gatcgcacat tcactcgcat cgatcagtcg atcgcgatgg ccgctctgtt caccgcgtac
     2641 catctgaagg tcaaggcgat agccgcgctg acgcagtcgg ggtcgactgc gttgtggatg
     2701 tcccggcata actgcggctg ccctattttc gcactgaccc cggaagtatc gtcgcagcgc
     2761 aggatggcgc tttatcgcaa t
//
LOCUS       DATANS010000597         1511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_451155, whole genome shotgun sequence.
ACCESSION   DATANS010000597 DATANS010000000
VERSION     DATANS010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451155"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VHT22_08785"
     CDS             <1..652
                     /locus_tag="VHT22_08785"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX3137693.1"
                     /translation="MFPESKLAQTSAGMMGGRAKLTGSGSSLGQMLGTANGEAALIMD
                     GGTVSELLLRLSNLDIANSVIVFLGGDKKVPIRCMVSNFKAVNGDFQVESLLLETPKV
                     DVTGSGDINLADETLRLRLVSESESFSLASLRGPIVITGSLKKPAVRPEMGNVVARGG
                     LAVVLGVITAGLGALLPLLEFGKRKQPSACALLMNQAKADAGVKASDLTPRNNRKK"
     gene            649..>1511
                     /locus_tag="VHT22_08790"
     CDS             649..>1511
                     /locus_tag="VHT22_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX3137694.1"
                     /translation="MRLTPAWRTAAASALLVAAGCATLPDARLYMEGRNAAPVQVSGA
                     RGPLSPEKSAAIIADLKRQSGDVDILQKHVALEQAIVGTPLVAGNRAVLLEDGPATYR
                     AMLAAISAAKDHINLETYIIEDDEMGRQFADLLLARQAAGVQVHLIYDSVGCIRTPRE
                     FFERLKEGGVQVLEFNPVNPLTAKKGWQVNNRDHRKLLLVDGRTAFIGGINISNVYSS
                     GSSPTRSTPSSPKSGGWRDTQIQIDGPVVAEFQKLFLATWEKQHGPPLAGKNYFPALS
                     PRGNELVRSIG"
BASE COUNT          300 a          482 c          482 g          247 t
ORIGIN      
        1 gatgtttccc gagtccaagc tcgcgcaaac gagtgcgggc atgatgggcg ggcgcgccaa
       61 gctgaccggc agcggcagtt cgctcgggca aatgctgggg accgccaacg gcgaagccgc
      121 gctgatcatg gacggcggca ccgtcagcga attgctgttg cggctgtcga atctcgatat
      181 cgccaactcc gtcatcgttt ttctgggcgg cgacaaaaaa gtgccgatcc gctgcatggt
      241 gtcgaatttc aaagcggtta atggcgattt tcaggtcgaa tcgctgctgc tcgaaacgcc
      301 caaggtcgat gtcaccggca gcggcgacat caatcttgcg gacgaaacgc tgcgcctgcg
      361 gctggtctcg gaatccgaga gcttcagcct cgcgtcgttg cgcggcccga tcgtcattac
      421 cgggtcattg aaaaaaccgg cggtgcgtcc cgagatgggc aacgtcgtgg cgcgcggcgg
      481 gctggccgtc gtgttgggcg tgattacggc aggcctgggc gcactgctgc ccttgctcga
      541 attcggcaaa cgcaagcagc ccagcgcctg cgcgctgctc atgaaccagg ccaaggcgga
      601 cgccggcgtc aaagcgagcg acttgacccc acgcaacaat cgcaaaaaat gaggctgacc
      661 ccggcgtggc ggacagcggc cgcgagcgca ctgctcgtcg cggcaggctg cgcgacattg
      721 cccgatgcgc ggctctacat ggagggccgc aacgcggcgc ccgtgcaggt gtccggcgcg
      781 cgcggcccgt tgtcgcccga aaaatcggcc gccattatcg ccgatctgaa acgccagtcc
      841 ggcgacgtcg acatattgca aaaacatgtc gcgctggaac aggcgatagt cggcacgccg
      901 ctcgtcgcgg gcaaccgcgc ggtcctgctg gaggacgggc cggcgaccta tcgcgcgatg
      961 ctggccgcca taagcgccgc caaagaccac atcaacctcg agacctacat catcgaggac
     1021 gatgaaatgg gccgccagtt cgcggacctg ctgctggcga ggcaggccgc cggcgtgcag
     1081 gtccacctga tttacgacag tgtcggctgt atccgcacgc cgcgcgaatt tttcgagcgc
     1141 ctgaaagagg gtggagtcca ggtgctcgaa ttcaatccgg tcaatccgct gaccgcgaag
     1201 aaaggctggc aggtcaacaa ccgcgaccac cgcaagctgc tgctggttga cggccgcacc
     1261 gccttcatcg gcggcatcaa catcagcaac gtttattcga gcggctcgag ccctacgcgt
     1321 tccacgccgt catcgccgaa gagcgggggc tggcgcgaca cccagatcca gatcgacggt
     1381 ccggtcgtcg cggaattcca gaaactcttt ctggcaacct gggaaaagca gcacggcccg
     1441 ccgctcgccg gaaaaaatta tttccccgca ttgagcccgc gcggcaacga actcgtgcgc
     1501 agcatcggca g
//
LOCUS       DATANS010000598         2558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_576037, whole genome shotgun sequence.
ACCESSION   DATANS010000598 DATANS010000000
VERSION     DATANS010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576037"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VHT22_08795"
     CDS             <1..588
                     /locus_tag="VHT22_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX3137695.1"
                     /translation="VDFVTLVGQAGTGKTLLTLAAGLMLTLEFKIYTEIIMTRVTVPV
                     GEDIGFLPGTEEEKMNPWMGALEDNLDVLNKTDDEAGEWGRAATRDLIRSRIKVKSLN
                     FMRGRTFINKFLIIDEAQNLTPKQMKTLITRAGPGTKVVCLGNIAQIDTPYLTEGSSG
                     LTYVVDRFKGWSHSGHVTLTRGERSRLADHAAEIL"
     gene            603..1592
                     /locus_tag="VHT22_08800"
     CDS             603..1592
                     /locus_tag="VHT22_08800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006515020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3137696.1"
                     /translation="MSLPSELAARDLDRPEVSIVVPVYNEEAGLAAFWTRLAPVLASL
                     TGSAEVIFVDDGSSDGTLAQLLSLRHLDRRVRIVSLSRNFGKEIALTAGLDHAGGDAV
                     IPIDADLQHPPEVIGELVARWHDGSDIVIALRRDRATDGILRRAGSSLFHRVFARMTT
                     VPVTRDAGDFRLMSRKVVEALRRLPERTRFMKGLYAWVGFRQTTVSYDIEPRLEGRSK
                     YGVWRLWRLAIDGITSFSSLPLKVWSVVGLVFALAALGYGVYLIARTIVRGVDVPGYA
                     SLMVVVLFLGGLQLLSLGIIGEYLGRVYDEVKARPLYVVRQRIGFDRDDPGAG"
     gene            1606..2001
                     /locus_tag="VHT22_08805"
     CDS             1606..2001
                     /locus_tag="VHT22_08805"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX3137697.1"
                     /translation="MPTLLHRFGRFAVVGALATASHAAVFAAAIEAAHVEPVLATALA
                     FVVAMLAGFALNRRWTFAVRDAPAQRLWRYAFAALAGLALNTAIMYCVVHLLHASPYL
                     GLAAAIVIVPPVSFTLNHFWVFRRPAPRG"
     gene            2012..>2558
                     /locus_tag="VHT22_08810"
     CDS             2012..>2558
                     /locus_tag="VHT22_08810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3137698.1"
                     /translation="MSYSIDVPVRRYATARRPSRADMAVVGAFMAIYIASSYFGAAGP
                     DTTRDVAAALAIRDLGALPLHGPLLAGTAHLGPLWFYLLSLPMLLARSWVGVAVFVAV
                     IGSLQFPLAYAAGRRLIDRRFGILWCALLTLPGWGSFQLVGFSHTNMVPTCSMLALYA
                     LVRLAQQRRPRWLVVSALAFSL"
BASE COUNT          374 a          852 c          861 g          471 t
ORIGIN      
        1 gtcgacttcg tcacgctcgt cgggcaggcg ggcacgggca agacgctgct gacgctggcg
       61 gcgggcctga tgctcacgct cgagttcaag atctacaccg agatcatcat gacgcgcgtc
      121 acggtgccgg tcggcgagga catcggcttc ctgccgggga ccgaagagga aaagatgaac
      181 ccgtggatgg gcgcgctgga ggacaatctc gatgtgctca acaagaccga cgacgaggcc
      241 ggcgaatggg gccgcgcggc gacgcgcgac ctgatccgct cgcgcatcaa ggtcaagagc
      301 ctcaacttca tgcgcgggcg cacgttcatc aacaagttcc tgatcatcga cgaggcgcag
      361 aacctgacgc cgaagcagat gaagacgctg atcacacgcg ccggaccggg caccaaggtc
      421 gtctgcctgg gcaacatcgc gcagatcgac acgccgtatc tcaccgaggg cagctccggg
      481 ctgacctatg tcgtagaccg cttcaagggc tggtcgcaca gcggtcacgt gacgctgacg
      541 cgcggcgagc gctcgcgact cgccgaccac gcggcggaaa tcctgtagcc gcggactccg
      601 cgatgagctt gccgtcggag ctggccgccc gtgatttgga ccggcccgag gtcagcatcg
      661 tcgtgccggt ctataacgag gaagcgggac tcgcggcatt ctggacccgg ctcgcgccgg
      721 tcctcgcctc tctcacgggc agcgcggaag tgatcttcgt cgacgacggc agcagcgacg
      781 gcacactggc gcaactgctg tcgctgcggc atctcgatcg gcgcgtgcgc atcgtcagct
      841 tgtcgcgcaa cttcggcaag gaaatcgcct tgaccgccgg tctcgatcac gccggcggcg
      901 acgcggtcat tccgatcgac gccgatctgc agcacccgcc cgaggtcatc ggcgaactcg
      961 tcgcgcgctg gcacgacggc agcgatatcg tcatcgcgtt gcgtcgcgac cgcgcgaccg
     1021 acggcattct gcgccgagcc ggctcatcgc tgtttcaccg agtttttgcg cggatgacga
     1081 cagtgccggt gacccgcgac gcgggggatt tccggctgat gtcccgcaag gtagtcgagg
     1141 cgctgcggcg gctccccgag cgcacccggt tcatgaaagg actctacgcc tgggtcggct
     1201 tccgtcagac caccgtgtcc tacgacatcg agccgcggct cgaaggccga tcgaagtacg
     1261 gcgtctggcg cctgtggcgg ctggcgatcg acggcatcac gtcgttcagc tcgctgccac
     1321 tcaaggtgtg gagcgtcgtc ggcctggttt ttgcgctcgc ggcgctgggc tacggcgtct
     1381 atctgatcgc gcgcacaatc gtgcgcggcg tcgatgtgcc cggctacgcc tcgttgatgg
     1441 tggtcgtcct gttcctcggc ggcctgcaac tgctttcgct gggcatcatc ggcgaatacc
     1501 tgggacgcgt gtacgacgag gtcaaggcgc ggccgctgta tgtcgtgcgg cagcgcatcg
     1561 gcttcgaccg cgacgatccg ggcgccggct gatccggcgc ggccgatgcc gaccctgctg
     1621 cacaggtttg gccgcttcgc ggtcgtcggc gcactcgcga ccgcgtcgca cgcggccgtg
     1681 ttcgccgcgg cgatcgaggc ggcccacgtg gaaccggtgc ttgctacggc gctggccttc
     1741 gtcgtcgcga tgctggccgg tttcgcgctg aaccggcgat ggacgtttgc cgtccgcgac
     1801 gcgccggccc agcggctgtg gcgctacgcc ttcgccgccc tcgcgggcct cgcgctcaat
     1861 acagcgatca tgtactgcgt cgtgcacctg ctgcacgcat cgccatatct cggccttgcg
     1921 gcggcgatcg tcatcgttcc gccggtaagc ttcacgctca atcatttctg ggtgtttcgt
     1981 cggccggcgc cgcgaggatg acgccaaagc gatgagctac tcgatcgacg tgccggtacg
     2041 gcgctatgcg acggctcgac gcccgagccg agccgacatg gcggtcgtcg gggcattcat
     2101 ggcgatttac atcgccagca gctatttcgg cgctgccggt ccggatacga cccgcgacgt
     2161 cgccgcggcg ctggcgatcc gcgatctcgg cgcactgccg ctgcacgggc cgctgctcgc
     2221 cggcactgcg cacttgggac cgctgtggtt ctatctgtta tcgctgccga tgctactggc
     2281 ccgctcgtgg gtcggcgtcg ccgttttcgt tgccgtcatc ggcagcctgc aatttccgct
     2341 ggcttatgcg gccggacggc ggctgatcga tcggcgtttc ggcatcctct ggtgcgcgct
     2401 gctgacgctg cccggatggg gaagctttca gctggtcggc ttttcgcata ccaacatggt
     2461 gccgacctgc agcatgctgg cgctgtacgc gctggtgcgc ctcgcgcagc agcggcgccc
     2521 gcgctggctg gtggtctcgg cgcttgcatt ttcactgg
//
LOCUS       DATANS010000599         1529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_746532, whole genome shotgun sequence.
ACCESSION   DATANS010000599 DATANS010000000
VERSION     DATANS010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746532"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VHT22_08815"
     CDS             <1..464
                     /locus_tag="VHT22_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3137699.1"
                     /translation="FQALQADGNIRAWGVSNFDSSDIEELLALRGGDDCAANQVLYNL
                     GARGVEWDLVPLCRGAGIALMAYSPLDQGRLLRERALQTIAAKIGATPAQVALAWLLQ
                     QRDVAVIPKAADVAHVHDNLSAARLHLDRAACEELDRTFPPPRRATSLRVL"
     gene            690..1010
                     /locus_tag="VHT22_08820"
     CDS             690..1010
                     /locus_tag="VHT22_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX3137700.1"
                     /translation="MLYTYYDYLDLPPGASAARIEAAYQTIKQRLDARTDETLVRLIH
                     EAHALLSDPDQRHVYDEELERSAAEADAELKALLDEQAGKPRTRRVQDVPTPLLAAIR
                     AWAA"
     gene            1290..1484
                     /locus_tag="VHT22_08825"
     CDS             1290..1484
                     /locus_tag="VHT22_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137701.1"
                     /translation="MVEGIWPVVRDDDWCGEWFAPAPSVLPRTHGIINGQELMAASAA
                     DSASRSPPEFRPLAAVAGDD"
BASE COUNT          284 a          476 c          508 g          261 t
ORIGIN      
        1 cattccaggc gctgcaggcg gacggcaaca tccgcgcctg gggcgtttcg aacttcgaca
       61 gcagcgatat cgaggaactg ttggcgctgc gcggcggtga cgattgcgca gccaatcagg
      121 ttctctataa cctcggcgcg cgcggcgtcg aatgggatct cgttccactt tgtcgcggcg
      181 ctggcatcgc gctgatggcg tattcgccgc tggaccaggg acgattgctg cgggagcggg
      241 cgctgcaaac gatcgcggcg aagatcggcg caacgccggc gcaagtcgca ctcgcgtggc
      301 tgctgcagca acgtgacgtt gccgtgatcc cgaaggcggc agacgttgcg catgtgcacg
      361 acaacttgtc ggcggcgaga ctgcaccttg atcgagccgc atgtgaagag ctcgatcgca
      421 cgtttcctcc accgcgccgc gcgacttctt tgcgcgtgct ttagcacctc gccttgccgc
      481 agggcggcag gccggtcgac gttgcgtcag gacccgcatg acaccgcgcg agcacgggcg
      541 ggcagcggcg cgggcggcag cttcactcaa acaatcaggc gtttgtgtgt ccgacgactg
      601 ctcagccggc gcccatggca tggatgttgc gtagccatgt ccatcagggc ctcggcccag
      661 ccgaattcaa actatttagg gagttagtcg tgctttacac ctactacgat tacctcgatt
      721 taccgccggg tgcatcggcg gcgcgcatcg aagctgctta ccagacgatc aagcagcggc
      781 tggacgcgcg caccgacgag acactggtgc gattgattca cgaggcgcac gcgctactgt
      841 cggaccccga ccagcggcat gtgtatgacg aggagctcga gcgcagcgca gccgaggccg
      901 atgccgagct caaggcgctg ctcgatgaac aagcgggcaa gccccgcacg cgtcgtgtgc
      961 aggatgttcc gacgccgttg ctggccgcga tccgcgcctg ggcggcctag caccatcggc
     1021 cgttcggacg atccatcgga ctgaccagaa tcggcgcgca agtgacagta ttgactcgcc
     1081 gcggtgtgtg atcgagctgc ccttcgcggc gcagcgatcg aatacggaca aacaagcgga
     1141 agggcagtac cgtgaaaaaa tgccagaatt gccaattcta cgatcgcaag gacgggcgat
     1201 cgaccgacgg acgcacgact atgtggggtc agtgcagacg tcattcgccg ctgctcagtc
     1261 ctcagcctcc gcaaagcgcc aagacctatg tggtcgaagg catttggccg gtcgtgcgcg
     1321 acgacgactg gtgcggtgaa tggttcgcgc cggcgccgag cgttctgccg cggacacatg
     1381 gcatcatcaa cggacaggaa ctgatggccg ccagcgcggc ggacagcgca tcgcgcagcc
     1441 cgccggaatt tcgcccgttg gcggcggtgg ctggcgacga ctgaaaaccg gtgcatcctt
     1501 ccgcgcgccg ggtggcgcgc gaatgcctg
//
LOCUS       DATANS010000600         1530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_29729, whole genome shotgun sequence.
ACCESSION   DATANS010000600 DATANS010000000
VERSION     DATANS010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29729"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VHT22_08830"
     CDS             complement(<1..257)
                     /locus_tag="VHT22_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX3137702.1"
                     /translation="MQGSNKHTESRWQRVLRSLLYGHGVDRNVKAKARLGLAIVAFAG
                     IFSVIAVRLVMFATVPEGHGGRRSMVQDAVATARPDILDRNG"
     gene            complement(262..633)
                     /locus_tag="VHT22_08835"
     CDS             complement(262..633)
                     /locus_tag="VHT22_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137703.1"
                     /translation="MRLVNILVIAALILAASFVYKIKFDSTLQAERVAKLRGELRRER
                     NAIAVLRAEWARLDTPGRIQGLAERHLTLQPVKSAQFDKLDRLPERPPAAEPPAGKDP
                     IGAMLAPGADTTGSVLPPARR"
     gene            complement(636..>1530)
                     /gene="rsmH"
                     /locus_tag="VHT22_08840"
     CDS             complement(636..>1530)
                     /gene="rsmH"
                     /locus_tag="VHT22_08840"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX3137704.1"
                     /translation="YTRAILAAADCSVIGIDRDQTAIALGADLVGQAGGRLTLIEDRF
                     SNLDAITRDAGFAAVDGVALDLGVSSMQLDTARRGFSFRLDGPLDMRMGGEGPSAADV
                     VNKASERDLANIIFILGEERHSRSVARAIVKARGDAPFETTRALAATVASVVRSRPND
                     IHPATRTFQALRIFVNDELGELATALHAAERVIKPGGRLVVVSFHSLEDRIVKTFLVD
                     RSGSRGSSRHVPEVDRPAPTFRTLTKRPVTADDEEIAQNPRARSAKLRAAERTDAAAR
                     AETPSNLLPDLPSLADVAGGR"
BASE COUNT          271 a          472 c          520 g          267 t
ORIGIN      
        1 ccgttgcggt cgaggatgtc gggccgcgcg gtggccaccg catcctgcac catgctgcgc
       61 cggcctccgt gaccttcggg caccgtcgca aacatcacga ggcgcacggc gatcaccgag
      121 aagatgccgg cgaacgcaac gatggcgagg ccaagccgcg cttttgcctt cacgttgcga
      181 tcgacaccgt gcccgtagag caacgagcgc agaacgcgct gccagcgact ttcggtgtgt
      241 ttgttcgatc cttgcacgat gtcaccgccg cgcaggcggc agcacgctgc cggttgtatc
      301 ggcgccaggc gcaagcatcg cgccgatcgg gtctttgcca gctggcggtt cggcagccgg
      361 gggcctttca ggcagacggt cgagcttgtc gaattgagcc gatttgaccg gctgcagcgt
      421 gagatggcgc tcagcaagtc cctgaatgcg gccgggtgtg tcgagccgcg cccattcggc
      481 gcgaaggaca gcgatcgcgt tgcgttcgcg tcgcagttca ccgcgcaatt tggcgacgcg
      541 ctcggcctgc agggtcgaat cgaacttgat tttgtagacg aaggatgccg cgaggatcag
      601 cgcggcgatg acgagaatgt tgaccaagcg catcgtcagc gcccccctgc aacgtcggcg
      661 agggatggca ggtcgggcaa caggttcgac ggtgtctcag cgcgtgcggc tgcatccgtt
      721 cgctcggcgg cgcgcagctt ggcggagcgc gcacgcggat tttgcgcaat ttcctcgtca
      781 tcggccgtga caggccgctt cgtcagcgtg cggaacgtcg gcgcgggccg atccacctcg
      841 ggtacatgac gggagctacc gcggcttccg ctgcgatcga cgagaaatgt tttgacgatg
      901 cggtcttcga gcgaatgaaa cgaaacgacc accagtcgcc cgcctggttt gatgacgcgt
      961 tcggccgcgt gaagggctgt tgccaattcg ccgagctcgt cgttgacgaa gatgcgcagc
     1021 gcctgaaacg tgcgcgtcgc cggatggatg tcgttcggac gcgagcgtac gaccgacgca
     1081 acggtggccg cgagcgcgcg cgtggtttca aatggagcat ccccacgcgc tttgacgatg
     1141 gcgcgcgcga cggagcgcga gtgacgctcc tccccgagga tgaaaatgat attggcgaga
     1201 tcgcgctcac tcgccttgtt gacgacatcg gcagcgctcg ggccctcgcc tcccatgcgc
     1261 atgtcgagcg ggccatcaag gcggaatgag aatccgcggc gggcggtgtc gagttgcatc
     1321 gacgaaacgc caagatcgag cgcaacgcca tcgaccgccg caaaaccggc atcgcgcgta
     1381 atcgcatcga gatttgaaaa tctgtcttcg atcagcgtca ggcggcctcc ggcttgaccg
     1441 accagatcgg cgccgagcgc gatggcggtc tggtcgcgat cgatgccgat cacgctgcaa
     1501 tcggccgcgg cgaggatcgc gcgggtatag
//
LOCUS       DATANS010000601         3793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_825479, whole genome shotgun sequence.
ACCESSION   DATANS010000601 DATANS010000000
VERSION     DATANS010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825479"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..784
                     /locus_tag="VHT22_08845"
     CDS             110..784
                     /locus_tag="VHT22_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3137705.1"
                     /translation="MLVVHGLTKSYAGPRPRAVFADVDLDLSPGDYIAVMGESGVGKS
                     TLLNLIAGLDSPDRGSIRLGDVELTTLDDDALTALRRERFGFVFQAFHVLPYLSVARN
                     VALPLSLLGIAASETDARVTSVLAGVGLDGRGESMPRELSGGELQRVAIARALVHGPQ
                     LVLADEPTGNLDAETASQVLRLLRDQIKTKGAAGILVTHSRAAAQTCDRILQLTPTGL
                     RAPPSS"
     gene            787..3300
                     /locus_tag="VHT22_08850"
     CDS             787..3300
                     /locus_tag="VHT22_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX3137706.1"
                     /translation="MGKLNLSAARAVLAGSLVQNKARSALSVLAIALGVALGYAVSLI
                     TGAAVNELALGVQFLAGDADLQVRGPRGGFDQALYPQLARVAEVAVASPVVEADAKLG
                     DRDDVLKIIGIDVFRAGAVQPGLIAESGDRLDHLRSDTLFLSPAAAHSLGVDRGDTLA
                     FQAGLRVVTLRVAGLVPADVQQRFAVMDIAGVQSNFDRLGRITRIDLRLRPGVDIETV
                     RARIQAQLPAGVAVQRPETGVAASESLSRSYRVNLNVLALVALFTGGLLVFSTQALAV
                     VRRRSQLALLRVLGMTRRELASLLVLEGAIVGIVGSALGLATGFILAQIAVQIIGADL
                     GAGYFRGVAPALPLAPISLAIFFGLGVLIAMLASLAPALEAARAAPAAALKAGDEERA
                     FARLSPSWPGFATIGAGAIAALVPPVAGLPLFGYIAIALMLIGTLMLMPRIAVMLLAA
                     LPLPSGAPPRLALLQLRGAPGQVAVSLAAIVAAVSLMVSMAIMVASFRISLDVWLERV
                     LPADLYVRAGAGGDTAYLGTDDQARLAALPGVRRAEFQREQQLLLDPARPRIVLLARS
                     IDAVNLSRQLQLVSGPVTVAAGAPPTAWVNEAMVDLYGFVPGKIIELPIAGKRAAFTV
                     AGVWRDYARPQGAVVIERERYIALTGDRSVTGGALWLAAGANPEALGQAIARDIPGGA
                     RLEVAAPGEIREVSLKAFDRTFAVTYALELAAVVIGLFGLSSSFGALVLSRRREFGML
                     RHIGMTRRQIGAMLATEGLTISAVGLVAGFGLGWLISLVLIHVVNRQSFHWGMELHVP
                     WSALAGSAVVVLALSTVTALASGRQAMSRRAVLAVKEDW"
     gene            3329..>3793
                     /locus_tag="VHT22_08855"
     CDS             3329..>3793
                     /locus_tag="VHT22_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HEX3137707.1"
                     /translation="MRRRRFLASPLVLLAAGALAADDYPRVVTGLAIGFPRDAGAHPA
                     FRSEWWYITGWVRDADANRFGIQVTFFRNRPRVAESNPSAFAPRQLLFAHAAIADPRH
                     GRLRHDQRAARAGFELAGAAEDTTAAWIDDWSLKLAGDVYVAAIAARDFRFEL"
BASE COUNT          495 a         1295 c         1289 g          714 t
ORIGIN      
        1 tcagcgtctg aagacacgct tctgtcgcca acaatccgtg acagttaagg gacacaggca
       61 ccgttcgagc ccgcttgtca cggtgtcgtg cggccgctac gctggcacca tgttggtggt
      121 ccatggcctg accaagtcgt acgccgggcc ccggccgcgc gcggttttcg ccgatgtcga
      181 tctggatctc tctcccggcg actacatcgc cgtcatgggc gagtcgggcg tcggcaagtc
      241 cacgttgctc aacctgattg ccggcctcga cagccccgac cggggctcga tcaggctcgg
      301 cgatgtcgag ctcacaactc ttgacgacga cgcgctgacc gcgctgcgcc gcgaacgctt
      361 cggcttcgtg tttcaggcct ttcatgtgtt gccctacttg agcgtggcac ggaacgtcgc
      421 cttgcccctg tcgctgctcg gcatcgccgc gtcggagacc gacgcgcgag tgaccagcgt
      481 gctggccggc gtcggcctcg atggccgcgg cgaaagcatg ccgcgcgagc tttccggtgg
      541 cgagcttcag cgggtcgcaa tcgcgcgcgc gctggttcac gggccgcaac tggtgcttgc
      601 ggatgaaccg acgggcaatc tcgatgcgga gacagcaagc caggtgctgc gcctgctgcg
      661 agaccagatc aagaccaagg gtgcggccgg gatcctggtc acgcattcgc gcgcggccgc
      721 gcagacctgc gatcgcatat tgcagctgac tcccaccggc ttgcgcgcgc cgccgtcgag
      781 ctgacgatgg gtaagctgaa cctctccgca gcgcgcgctg tgcttgccgg ctcgctggtg
      841 cagaacaagg cgcgcagcgc cttgtcggtg ctcgccatcg cacttggggt cgcgctcggt
      901 tacgcggttt cgctgatcac cggcgccgcg gtcaacgagc tcgctctcgg cgtgcagttc
      961 ctcgccggcg atgccgatct ccaggtgcgc ggcccgcgcg gcggtttcga ccaggcgctc
     1021 tatccgcagc tggcccgggt ggcggaggtg gccgtggcga gtcccgtcgt cgaggcggat
     1081 gccaagctcg gcgatcgcga cgacgtgctg aagatcatcg gcatcgacgt cttccgcgcc
     1141 ggagccgtgc agcccgggct gatcgccgag tccggcgacc gtcttgatca tctgcgctcc
     1201 gatacgcttt tcctcagtcc cgccgcggca cactcgcttg gcgtcgatcg aggcgacacg
     1261 ctggcctttc aggcaggctt gcgcgttgtg accttgcgcg tcgcaggact cgtgcccgcc
     1321 gatgtgcaac agcgattcgc ggtgatggat atcgccggcg tccagtcgaa cttcgatcgg
     1381 ctgggccgca ttacccgcat cgacctgcga cttcggcccg gcgtcgacat cgaaaccgtg
     1441 cgcgcgcgca tccaggcgca gctgcccgcc ggcgtcgcgg tccaacgccc ggagacgggt
     1501 gtggccgcca gcgaaagcct gtcccgttcg taccgcgtca atctgaacgt gctggcgctg
     1561 gtggcgctgt tcaccggcgg cttgctggtt ttttcgacgc aggcgctcgc tgtcgtgcgc
     1621 cgccgctcgc agctggcgct gttgcgtgtt ctcggcatga cgcgccgtga gttggcgagc
     1681 ctgctcgtgc tcgaaggcgc aatcgtcggc atcgtcggca gcgcgctcgg tctggccact
     1741 ggattcatcc tcgcgcaaat cgcggtccag atcatcgggg ccgacctcgg cgccggatac
     1801 tttcgcggcg tcgcaccggc gctgccactg gctccgattt cgctggcgat tttcttcggt
     1861 ctcggcgtgc tgatcgcgat gctcgcgagt ctcgctcccg cgctggaggc cgcacgcgcg
     1921 gctcccgcag cggcgctcaa ggccggtgac gaagagcgcg cattcgcccg gctctctcct
     1981 tcgtggccgg gattcgcgac catcggcgcc ggggcgattg cggcgctcgt tccgccggtc
     2041 gcgggcttgc cgctgttcgg ctacatcgcg atcgcactga tgctgatcgg aacgctgatg
     2101 ctgatgccgc ggatcgcggt gatgctgctc gcggccttgc ctctcccgag cggcgcgccg
     2161 ccgcggctcg cattgctgca gctgcgcggc gcccccgggc aggtggcggt gagcctcgcc
     2221 gcgattgtcg ccgccgtcag tctgatggtc tcgatggcaa tcatggtcgc gtcgttccgg
     2281 atttcgctcg acgtgtggct cgagcgcgtg ctacctgccg atctctacgt gcgcgcgggc
     2341 gctggcggcg acaccgctta cctcggcacc gacgatcaag cacgccttgc cgcgctaccc
     2401 ggcgtgcgcc gtgccgaatt ccagcgcgag cagcaactgc tgctcgaccc tgcccggccg
     2461 cgcattgttc tgcttgcgcg cagcatcgat gcggtgaatc tgtcgcggca gcttcagctg
     2521 gtaagcgggc cggtcaccgt cgctgccggc gcgccgccga cggcgtgggt caacgaggcg
     2581 atggtcgatc tttacggttt tgtacccggc aagatcatcg agcttccgat cgcgggcaag
     2641 cgggctgcct ttaccgtcgc gggagtgtgg cgcgactacg cgcggccgca gggggcagtg
     2701 gtcatcgagc gcgagcgtta tatcgcgctg accggcgatc gaagtgtgac cggcggcgcg
     2761 ctatggctcg cagcgggcgc gaatccggaa gcgcttggcc aggcgatcgc gcgcgacatc
     2821 cccggtggtg cgagactcga ggttgccgcg cccggtgaaa tccgcgaggt atcgctgaag
     2881 gcattcgatc gaacgtttgc cgtcacctat gcgctcgagc tcgccgcggt cgtcatcggc
     2941 ctgttcggcc tgtcgtcgtc gttcggcgcg ctggttcttt cgcgccgtcg cgagtttggc
     3001 atgctgcgcc acatcggaat gacgcggcgg caaatcggcg cgatgcttgc caccgaaggg
     3061 ctgacgatca gcgccgtcgg cctcgtcgcc ggcttcggac tcgggtggct gatcagcctc
     3121 gtgctgatcc acgtcgtcaa tcgccaatcg ttccactggg gcatggagct ccacgtgccc
     3181 tggtcggcgc tcgcaggctc cgcggtggtc gtgctcgccc tttcgacggt caccgcgctc
     3241 gccagcggtc gccaggcgat gagccgtcgc gccgtcctgg cggtaaagga ggactggtga
     3301 tcgccgcaac aggcgcacga ggcgacgcgt gagacggcgc cggtttctcg cgagcccgct
     3361 cgtgctcctc gcggccggcg cgctcgctgc ggacgactat ccgcgcgtgg ttaccggcct
     3421 cgcgataggc tttccgcgtg acgcgggcgc gcatcccgcc tttcgcagcg aatggtggta
     3481 catcaccggc tgggttcgcg acgccgacgc caatcgattt ggcatccagg tcaccttttt
     3541 ccgcaatcgc ccgcgcgttg cggagtcgaa tccgagcgca tttgcgccgc gccagctgct
     3601 gtttgcgcac gccgccatcg ccgatccgcg ccatggcagg ctccgccacg atcagcgcgc
     3661 ggcgcgcgct ggcttcgagc tcgccggcgc ggcggaagac acgaccgccg catggatcga
     3721 cgactggtcg ctcaagcttg ccggagatgt ctacgtcgct gcgatcgcgg cgcgcgattt
     3781 tcgcttcgag ctt
//
LOCUS       DATANS010000602         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_238144, whole genome shotgun sequence.
ACCESSION   DATANS010000602 DATANS010000000
VERSION     DATANS010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238144"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VHT22_08860"
     CDS             <1..318
                     /locus_tag="VHT22_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX3137708.1"
                     /translation="GVLLALIVELLCCALTGARFGFENDSFFEPGRPASIGHALLAID
                     PGALAGMAAYQERMETLLAAMLVDDGVRLPGERRHKLTADALANGLAIPDAVLKQLRE
                     LAA"
     gene            complement(340..1068)
                     /locus_tag="VHT22_08865"
     CDS             complement(340..1068)
                     /locus_tag="VHT22_08865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018399655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3137709.1"
                     /translation="MRNRARDARKVFLWLLLALGLPAAAAETTAPQYSSVAPTPDGIG
                     KVYMGREISHVMGFHGAQWLERTERMDEERPDLVLNALDLKPGMTVADIGAGTGYYTW
                     RIAQRIGAAGTVYAVDVQPEMIKALERQMSRRGAANVKAVTGTPTDPKLPRNSLDLAL
                     MVDVYHEFEQPYEMLAAIVAALKPGGRVAFVEFRGNDPKVPIKPLHTMTEEQVRREAG
                     AHALEWIRTTHDLPWQHVIVFRKQ"
     gene            complement(1061..1786)
                     /locus_tag="VHT22_08870"
     CDS             complement(1061..1786)
                     /locus_tag="VHT22_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HEX3137710.1"
                     /translation="MHDDDRQNGRILNRREALALLGTAGAALLAARAPWVSAAEPALA
                     CVASPEQTEGPYFVDERLNRSDIRSDPSDGSVREGSPLTLSLVVSSVAAGKCAPLAGA
                     MVDVWHCDAAGVYSDVNDPGANTIGRKFLRGYQVTDSRGGVQFTTVYPGWYEGRAVHI
                     HFKVRGTAAAKRGYEFTSQFYFDDAFSDRVFARHPYGRHGQRAVRNERDGLFRRGGSQ
                     MILPVFESGTGYAANFNVGLRLA"
     gene            complement(1794..2177)
                     /locus_tag="VHT22_08875"
     CDS             complement(1794..2177)
                     /locus_tag="VHT22_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3137711.1"
                     /translation="MKVELDHTIVPSRDKVTAARTLGELLGVPWAAAGLGPFAPVYVN
                     EGLTLDFIDTDEAFPVYHFCFRVGSVEFDAILGRIKAAGIKYRSNVHGPVDMQVGAQY
                     GNFYWNEPDGHQWELLTTSYARPPV"
BASE COUNT          361 a          705 c          739 g          411 t
ORIGIN      
        1 ggcgtcttgc tcgcgctgat agtcgagttg ctgtgctgtg cgttgaccgg cgcgcggttc
       61 ggtttcgaga acgactcgtt cttcgagccg ggacggcccg cttcgattgg ccacgcgctg
      121 ctcgccatcg accccggcgc actggcaggg atggctgcgt accaggagcg catggaaacg
      181 ttgctggcgg cgatgctcgt cgatgatggt gtgcgtttgc cgggtgagcg gcggcacaag
      241 ctcacggccg atgcgcttgc gaacggcctt gcgattccgg atgcggtgtt gaagcagttg
      301 cgcgaacttg ccgcgtgacg cgttaatcgg ccgcgggatt tactgcttgc ggaacacgat
      361 cacgtgctgc cacggcagat cgtgcgtcgt ccgaatccat tcgagcgcat gcgcgccggc
      421 ttcgcgccgc acctgttctt cagtcatggt atgcaaaggc ttgatcggaa ccttaggatc
      481 gttgcccctg aactcgacga acgcgacccg cccgcccggt ttcaacgcgg ccacgatggc
      541 ggccagcatt tcgtagggct gctcgaactc atgatagacg tccaccatca gcgcgagatc
      601 gaggctgttg cgcggcagct tcggatccgt cggcgttccg gtcacggcct tgacgttggc
      661 ggcgccgcgt cgcgacatct gccgctcgag cgccttgatc atttcaggct gcacgtccac
      721 ggcatagacc gttcccgcgg cgcctatgcg ctgcgcgatg cgccacgtgt aatagccggt
      781 gcctgcgccg atgtcggcga ccgtcatgcc gggcttcaag tcgagcgcat ttagcaccag
      841 gtccgggcgt tcttcatcca tgcgctcggt gcgttcgagc cattgagcgc cgtgaaaacc
      901 catcacgtgc gagatttcgc ggcccatgta gaccttgccg atgccgtcgg gggtcggcgc
      961 aacggaggag tattgcgggg ccgtcgtctc ggccgcggcc gcgggcagcc cgagcgccag
     1021 gagcagccac aaaaaaactt tgcgcgcgtc gcgcgcgcga ttacgcaagc cttaacccga
     1081 cgttgaagtt cgccgcatag cccgtcccgc tctcgaagac cggcagtatc atctggctgc
     1141 cgccgcgccg gaagaggcca tcacgctcgt tgcgcactgc gcgttgtccg tggcggccgt
     1201 atgggtggcg cgcgaacacg cggtcgctga acgcgtcgtc gaaatagaac tgcgacgtga
     1261 attcgtagcc gcgtttcgct gcggccgtgc cgcgcacctt gaagtgaatg tgcaccgcgc
     1321 ggccctcgta ccagccggga tagacggtgg tgaactgcac gccgccgcgt gagtcggtga
     1381 cttgatagcc gcgcaggaat tttctcccga tcgtattggc gcccggatcg ttcacgtccg
     1441 aatagactcc cgccgcgtcg cagtgccaga catcgaccat tgcgcccgcg agcggcgcgc
     1501 acttgcccgc ggccaccgag gagaccacga gcgacagcgt cagcggcgag ccttcgcgca
     1561 ctgaaccatc ggatggatcg gatcggatgt cggagcgatt caatcgctcg tcgacgaaat
     1621 acggaccttc ggtctgctcg ggactcgcga cgcacgcgag cgcaggctcg gcggcggaca
     1681 cccacggcgc gcgcgcggcc agcagtgcgg ccccggcggt gccgagcagc gccagcgctt
     1741 cacggcggtt caggatgcgg ccgttttgac gatcgtcgtc gtgcattgga tgctcaaacc
     1801 ggcggccgcg cgtagctggt tgtcaacaac tcccactgat ggccgtcggg ctcgttccag
     1861 taaaagttgc cgtattgtgc gccgacctgc atgtcgaccg ggccgtggac gttgctgcgg
     1921 tatttgatgc ccgcggcctt gatccggccg aggatagcgt cgaattccac tgacccgacg
     1981 cgaaagcaga agtggtaaac gggaaaggcc tcgtcggtat cgatgaagtc gagcgtcagg
     2041 ccttcgttga cgtagaccgg ggcgaacggg ccgaggcccg cggccgccca gggcacgccg
     2101 agcagctcgc ccagggttcg tgccgcggtg accttgtcgc gcgacggaac gatggtgtgg
     2161 tcgagctcga ccttcatgac cttccctcga ggggctcgtc agttgccgaa cacggt
//
LOCUS       DATANS010000603         1526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_785769, whole genome shotgun sequence.
ACCESSION   DATANS010000603 DATANS010000000
VERSION     DATANS010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785769"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VHT22_08880"
     CDS             complement(<1..933)
                     /locus_tag="VHT22_08880"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX3137712.1"
                     /translation="MPVSAMTMAAPYQRLGEDGYAFRTLCVLMTILVAILPLAHVTGM
                     RNALVGMAAVAALFHYRAQLWKDNPVLLPSLVWLAFAALSIAWSEYPMVSFRAFRSDL
                     WYPLILFLISFMLVRSRNGRLALTLGAAIGTVISLTTTFAAAVMPVEPGMESAAPGVL
                     GWLAWKAGEAVDSSTYIAFLAVPLFVVALTSTRASVRGASAAWLVAFAGLGLMSESRT
                     LFVSLCISLPCLLIVFGLLRGKLHVRPVLVAICVGLVVSAISLELISRLRLPEQQPYD
                     RSAALEMIASDSRPAIWAVYYDLAKRHPWLGVGFG"
     gene            complement(962..1034)
                     /locus_tag="VHT22_08885"
     tRNA            complement(962..1034)
                     /locus_tag="VHT22_08885"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(999..1001),aa:Phe,seq:gaa)
     gene            complement(1098..>1526)
                     /locus_tag="VHT22_08890"
     CDS             complement(1098..>1526)
                     /locus_tag="VHT22_08890"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX3137713.1"
                     /translation="GRPTTDPKDFYDGGALLTVGAHKGSGLSIVVDLLAGALSTGKSS
                     DPDDTVLRNNMLSIYIAPAVYDEDGAIAREIARFVAWVKASPPAVPGQPVLMPGEVER
                     STRGRRTLEGIAVDDKTWMDILDAAKSVGIAATRAEAIVA"
BASE COUNT          316 a          488 c          490 g          232 t
ORIGIN      
        1 tccgaatccg acgcccagcc aggggtgccg tttggcaaga tcatagtaga cggcccagat
       61 cgccgggcgc gagtcgctgg cgatcatctc cagcgccgcg cttcggtcat agggttgttg
      121 ctcgggcagg cgcaagcgcg aaatcagctc gaggctgatc gccgagacga ccaggccgac
      181 acagatcgcc acgagcaccg ggcggacatg cagctttccg cgcagcaatc cgaacacgat
      241 cagcaggcat ggcagcgaaa tgcagagaga aacgaacagc gtccggcttt cgctcatcag
      301 cccgaggccg gcaaaggcga cgagccaggc ggccgaggcg ccgcgtaccg acgcgcgggt
      361 cgacgtcagc gcgacgacga acagcggcac cgcgagaaaa gcgatgtagg tgcttgaatc
      421 gaccgcttcg cccgctttcc atgcgagcca gccgagcacg ccgggcgcgg ctgattccat
      481 tcccggctcg accggcatta ccgccgcagc gaaggtcgtc gtcaacgata tgacggtgcc
      541 gatggcggcg ccgagagtca gcgcgagcct gccgttacgc gatcggacca gcatgaagct
      601 gatcaggaac aggatgagcg gataccaaag gtcgctgcga aaagcgcgaa acgagaccat
      661 cgggtattcc gaccaggcga tcgagagcgc ggcgaacgcc agccagacga ggctaggcaa
      721 caggactgga ttgtccttcc acaattgagc ccggtagtgg aacagcgcgg cgaccgcggc
      781 catcccgacg agggcattgc gcatgccggt cacatgcgcc agcggcagga tggcgaccag
      841 aatcgtcatc agcacgcaaa gcgtgcggaa cgcatagcca tcctctccca gccgctggta
      901 cggcgctgcc atggtcatcg cggataccgg catggaagga cgggattcaa tgcgaaatgg
      961 gtggccaagg gcggaattga accaccgaca cacggatttt cagtccgctg ctctaccaac
     1021 tgagctactt ggccaagcat tcaattttac cagagttgcc tctgccggca cgaggcgcga
     1081 tagggtcaat gcctcggtca ggcgacgatt gcttcggcac gagtggccgc gatgccgact
     1141 gatttcgccg cgtcgagaat gtccatccag gtcttgtcgt cgacggcgat gccctcgagc
     1201 gtccgtcgcc cccgcgtgct gcgctcgact tcgccgggca tcagcaccgg ctgccccggc
     1261 acggccggcg gcgacgcctt gacccacgcc acgaagcgcg cgatctcgcg cgcgatcgcg
     1321 ccgtcctcat catagaccgc tggagcaata tagatcgaca gcatgttgtt gcggagtacc
     1381 gtatcgtccg gatccgagct cttgccagtc gaaagtgcgc cggccagcag gtcgacgacg
     1441 atcgacaatc ccgagccctt gtgggcgccg acggtgagca gtgcgccgcc gtcgtagaaa
     1501 tccttcggat cagtcgtcgg ccggcc
//
LOCUS       DATANS010000604         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_576582, whole genome shotgun sequence.
ACCESSION   DATANS010000604 DATANS010000000
VERSION     DATANS010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576582"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..>1502
                     /locus_tag="VHT22_08895"
     CDS             191..>1502
                     /locus_tag="VHT22_08895"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3137714.1"
                     /translation="MTHWTRETDVVVIGGGLAGYCAALEAAAANADVLLLEKQPQVGG
                     SSVLSGGFFAFAGTDMQKAAGIADSNERLFDDLRKAGGGENDEKLLNVYVEQQLATYE
                     WLKSIGVTFRTVELSSAQSVPRTHPLDPRALIDRLRAMADKNGRITTLLDAPAVRLLQ
                     DEPGGRVTGVKAVIAGNPETIAARRGVVLAAGGFSRNAAMLKQYVPHQAAGVSYGGAG
                     NMGDGIRMACALGAGVRDMGFIKGTFGYHPNARTQEGRDWTKLPVYRGGIAVNKLGTR
                     YVDESKSYKLLGDAVLDQPGAIAYQIFDQNIMDGASDGVPPFDFRSTQRRGLVFEAPT
                     LAALAEKTGVDATQLATTVARYNGFVESGVDADFGRDGLSTHYGKLVKIERAPFYAYP
                     STSGIIATYCGLTVEADTRVLNVFGESIPGLYAAGELMGGFHGVS"
BASE COUNT          300 a          437 c          510 g          255 t
ORIGIN      
        1 gcaatctctc ccagacgatg gcaaatatct acatggacaa ggaattcaac gagcgctgcg
       61 cgacggtcgg ggtggtgcca ctcaaaggca atccaacgga gttcgcgcgg ttcattcagt
      121 cagagctctc gaaatgggga aaagtcgtgc gtgacgcggg cgcacgcatc gattagaaag
      181 gcacgcagca gtgactcatt ggacgcgcga aacggatgtc gttgtaatcg gcggcgggct
      241 cgcgggctat tgcgcggcgc tcgaggccgc tgcggcaaac gccgacgttc ttttattgga
      301 gaagcagccg caagtcggcg gcagcagcgt gctgtcgggc ggattctttg cgttcgccgg
      361 caccgatatg cagaaggccg cgggcatagc cgacagcaac gagcggctct tcgacgattt
      421 gcgcaaggcc ggcggcggcg agaacgacga aaagctcctc aatgtctatg tcgaacagca
      481 actcgcgacg tacgagtggc tcaaaagcat cggcgtgaca ttccggacgg tggaactgag
      541 ctcggcgcag tcggtgccgc gcacgcatcc gctcgacccg cgggcgttga tcgaccgcct
      601 gcgcgcgatg gcagacaaaa acgggcgtat caccacgctg ctcgacgcgc ccgccgtgcg
      661 gctcttgcag gatgaacccg gcgggcgcgt gaccggcgtg aaagcggtca tcgcgggcaa
      721 tccggaaacg atcgccgcgc ggcgcggcgt agtcttggca gcgggcggct tctcgcgcaa
      781 cgcggcgatg ttgaaacagt atgtgccgca tcaggcggcg ggcgtgagtt acggcggcgc
      841 gggcaatatg ggcgacggca tccgcatggc gtgcgcgctc ggcgcaggtg tgcgcgacat
      901 gggctttatc aagggcacgt tcggatatca tccgaacgca cgcactcagg aagggcgcga
      961 ctggacgaag ctgccggtgt atcgcggcgg catcgcagta aacaagctgg gcacgcgcta
     1021 tgtggacgaa tcaaaatcgt acaagctgct cggcgatgcg gtgctggacc aacccggcgc
     1081 aatcgcatat cagatattcg accaaaacat catggacggc gcgtccgacg gggtgccgcc
     1141 gttcgatttc aggagcacgc agcggcgcgg gctcgtgttc gaagcgccga ccttggccgc
     1201 actcgctgaa aaaaccggcg tcgatgcgac gcagctcgca acgacggtcg cgcgctacaa
     1261 cggcttcgtc gagtcgggcg tcgacgcgga tttcggccgt gacggcctgt ccacgcacta
     1321 tggcaagctc gtgaaaatcg agcgcgcacc gttctacgcc tatccgtcga cgagcggcat
     1381 catcgcgacg tattgcgggc ttacggtcga agcggacacg cgcgtgctga acgtttttgg
     1441 tgaatccatc cccggtctgt acgcggcagg tgagttgatg ggcggttttc acggggtctc
     1501 at
//
LOCUS       DATANS010000605         2007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_785887, whole genome shotgun sequence.
ACCESSION   DATANS010000605 DATANS010000000
VERSION     DATANS010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785887"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1414
                     /gene="zwf"
                     /locus_tag="VHT22_08900"
     CDS             38..1414
                     /gene="zwf"
                     /locus_tag="VHT22_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX3137715.1"
                     /translation="MSAQSCDALVFFGATGDLAYKKIFPALQAMVKRGTLDVPVIGVA
                     KSGWGLEQLKARARESIDKHGGIDAPAFGKLMELLRYVDGDYADGGTFASLRKLLGNA
                     ARPAHYLAIPPALFGNVVQHLARAGCAPAGSRVVVEKPFGRDLTSAQALNGILLRTFD
                     EQHIFRIDHYLGKGPVRHMAFFRFSNALLESFWSRQHISSVQITMAEDFGVQGRGGFY
                     EQTGAIRDVVQNHLFQLLATLTMEPPARIDSESIRDEKVKVLKSIAPIEPRELVRGQF
                     QGYHNEPGVAADSRVETFAALRLRIDSWRWQGVPFYIRTGKSLPVTCTEVFARLRRPP
                     ALFAARPAPNYLRFRISPVVEFALGVNVMDPEDKGDGTIAELLASRRPGSNDIDAYER
                     VLTDAMEGDPTLFAREDYIEEAWRIVDPVVRAGATVYEYAPGTWGPKEVASVTPPGGW
                     QDPLVTSA"
     gene            complement(1425..>2007)
                     /locus_tag="VHT22_08905"
     CDS             complement(1425..>2007)
                     /locus_tag="VHT22_08905"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3137716.1"
                     /translation="MGLLYATGTGVPQDYIAALAWYQRAAEGGSTSAMNNIATIYFHG
                     LGVPQSYEETVKWLRLAVKQGDATAQNRLGTMYDAGLGVTRNAHDAFELFTQAANQGY
                     APGMANLGRAYARGAGVKKDEPRAYTLISAAMEIGLRAGERDAAMYELGALSQRLDAK
                     QIERAQLDARAMVETRSKEASAREVTASNAYRL"
BASE COUNT          337 a          653 c          646 g          371 t
ORIGIN      
        1 gctatcaatt cgggggccat ctcgagaagc cttcctcatg agcgcgcagt cctgtgacgc
       61 gctggtcttc ttcggcgcca ctggcgatct cgcgtacaag aaaatcttcc cggccctcca
      121 ggcgatggtg aagcgcggca cgctcgacgt gcccgtcatc ggcgtcgcca agagcggctg
      181 gggactcgaa cagctgaagg cgcgagcacg cgagagcatc gacaagcacg gtggcatcga
      241 tgccccggcc ttcggcaagc tcatggagct gctgcgctac gtcgacggcg attatgcaga
      301 tggcggaacg ttcgcatcgc tgcgcaagct gctgggcaac gctgctcgcc ctgctcacta
      361 tctggcgata ccgcctgcgc tgttcggcaa tgtcgtccag cacctcgcac gggcaggctg
      421 cgcgccagcc ggttcgcgcg ttgtcgtcga gaagccgttt ggccgcgatc ttacttccgc
      481 gcaagcgctc aacggcatcc tgctgcgaac gttcgacgag cagcacatct ttcgcatcga
      541 tcattacctc ggcaagggac cggtgcgaca catggcgttc tttcgcttca gcaatgcgtt
      601 gctggagtcg ttctggagcc gccagcacat ctccagcgtg cagatcacga tggcggagga
      661 tttcggcgtc cagggacgcg gcggcttcta cgaacagacc ggcgccattc gcgacgtcgt
      721 gcagaatcat ctgttccagt tgctagcgac gctcacgatg gagccgccgg cgcgcatcga
      781 cagcgaatcc atccgcgacg aaaaagtgaa ggtgctgaag tcgatcgcgc cgatcgagcc
      841 gcgcgagctg gtgcgcggtc agttccaggg ctaccacaac gagcccggtg tcgccgctga
      901 ttcgcgggtt gaaacctttg cagcgttgcg cctccgcatc gacagctggc gctggcaggg
      961 cgtgcccttc tacattcgca cgggcaagtc gctgccggtg acgtgtacgg aagtcttcgc
     1021 gcggctgcgc cggccacctg cattgttcgc ggcacgcccc gcgccgaact acctgcgatt
     1081 ccgcatcagc ccagtcgtcg agttcgcgtt gggcgtcaac gtgatggatc ccgaggacaa
     1141 gggtgacggc acgatcgccg agctgctcgc gagccggcgc cccggcagca acgacatcga
     1201 cgcgtacgag cgcgtgctga ccgacgcgat ggagggcgat ccgaccttgt tcgcgcgcga
     1261 ggactacatc gaagaagcgt ggcggatcgt cgacccggtc gtgcgggccg gcgccacggt
     1321 gtacgaatac gcgcccggaa cgtggggccc gaaggaagtt gccagcgtca caccgccggg
     1381 cggctggcag gatccgctcg tcacgtcagc ttaagccgtc ctgctcagag gcggtaagcg
     1441 ttgctcgcgg tgacttcgcg ggcagaggct tccttcgatc gcgtctcgac catggcacga
     1501 gcgtcgagct gtgcgcgctc gatctgcttc gcatcgagcc gctgcgaaag cgcgcccagt
     1561 tcgtacattg cggcgtcacg ctcaccggct cgcagtccga tttccatggc agcagaaatg
     1621 agcgtgtagg cccgtggctc atccttcttg acgccagcgc cgcgggcgta agcgcgcccg
     1681 aggtttgcca tgcctggcgc gtatccttgg ttcgcagcct gagtgaacag ctcgaacgca
     1741 tcgtgagcgt tcctggtgac gcccagcccg gcgtcataca tcgtgccgag ccggttctgg
     1801 gcggttgcgt cgccctgctt caccgcgagt cgcagccact tcactgtctc ttcgtagctc
     1861 tgaggcacgc cgagaccatg gaagtagatg gtcgcgatgt tgttcatcgc gctggtcgaa
     1921 cccccttccg ccgcgcgctg ataccacgcg agtgcggcga tgtagtcctg cggcacgccg
     1981 gttccggtcg catacagcag gcccatg
//
LOCUS       DATANS010000606         1806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_785910, whole genome shotgun sequence.
ACCESSION   DATANS010000606 DATANS010000000
VERSION     DATANS010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785910"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..1549
                     /gene="murJ"
                     /locus_tag="VHT22_08910"
     CDS             14..1549
                     /gene="murJ"
                     /locus_tag="VHT22_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX3137717.1"
                     /translation="MNLLRTLSAVSALTLLSRITGLMRESIKAVGFGAGLQMDAFEAA
                     FRLPNILRRMFAEGAFSQAFVPILADYKRSRGEAATRDLVGKVGSVLAVTLIIISVFG
                     IVAAPWLVYLLAGSFVRTPGKVELTTVLIRIMFPYVLFVSLVSLAGGVMNVYRQFAIP
                     AFTPVLLNVAIIAAAVFVAPHVDPPILALAIGVFVGGVAQLIFQLRPLARLGMLPSFT
                     LDWRDEGVRRVLRAMGPAVLGVSASQISTLINTQLAAYLGDGRISWITYADRLMEFPS
                     ALLGVALATILLPALSKHHHDANDAQYRELLDWGLRLTFLLALPAALALALLAVPLIS
                     TLYQYGRFTAVDVWQTRAALLGYSVGLLGLIAVKILAPGFYARHDMRTPVRIAFATLL
                     VSQAMAVILMFQIGHAGLTLSISLGACLNAALLYRAMRRRDVYRPLPGWGRFLWRIAV
                     ALCVLGAVLWWTAGDDAFWLAAGLWAKVGRLGMIIAAGAVAYFGSLWLVGFRLADFNR
                     REG"
     gene            complement(1551..>1806)
                     /locus_tag="VHT22_08915"
     CDS             complement(1551..>1806)
                     /locus_tag="VHT22_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX3137718.1"
                     /translation="QAGLLVRHHFESGKAVFELNQGGHHDHLVCLQCGRVEEFFDAEI
                     EKRQTKIARERGFEISEHALYLYAECTRPKCPYRKTGGSN"
BASE COUNT          237 a          594 c          594 g          381 t
ORIGIN      
        1 cgccggccct cgcatgaatc tgctgaggac gctctccgcc gtcagcgcgc tgaccttgct
       61 gtcgcgcatc accggactga tgcgcgagag catcaaggcg gtcggtttcg gcgccggatt
      121 gcagatggat gccttcgaag cggcgttccg cttgccgaat atcctgcgcc gcatgtttgc
      181 cgaaggcgcg ttctcgcagg cattcgtacc gatcctcgcc gactacaagc gctcgcgcgg
      241 cgaggccgcg acccgcgatc tggtcgggaa agtcggatcg gtgctcgcgg tgactctgat
      301 tatcatatcg gtattcggca ttgttgccgc accctggctg gtctatctgc tggccggcag
      361 cttcgtgcga acgccgggca aggtcgagct gaccacggtg ctgatacgga tcatgttccc
      421 gtatgtgcta ttcgtgtcgc tggtttcgct ggccggcggg gtgatgaatg tctatcgcca
      481 gttcgcgatc cccgcgttca ccccggtgct gctcaacgtc gcgattatcg ccgccgccgt
      541 ttttgtcgcg ccgcatgtcg acccgccgat cctcgcgctg gccatcggcg tgtttgtcgg
      601 cggcgtcgcg cagctgatct ttcagctgcg cccgctggct agattgggca tgctgcccag
      661 cttcacgctc gactggcgcg acgagggcgt gcgccgcgtt ctgcgcgcga tgggcccggc
      721 ggtgctcggc gtttcggcgt cgcagatttc gaccttgatc aacacgcagc tcgccgccta
      781 tctgggcgac gggcggatat cgtggatcac ctacgccgac cggctgatgg aattccccag
      841 cgcgctgctc ggcgtcgcac tcgcgacgat cctgctgccc gccctttcca agcatcacca
      901 cgacgcgaac gacgcgcagt atcgcgagct gctcgactgg ggtctgcgac tgacattcct
      961 gctcgcgttg ccggcggcgc tcgcgctggc gctgctggcc gtgccgttga tctcaacgct
     1021 ttatcaatac ggccgtttca ccgccgtcga cgtctggcag acgcgcgccg cgctgctcgg
     1081 ctacagcgtc ggcctgctcg gactgatcgc ggtgaaaatc ctggcgccgg gcttttatgc
     1141 gcggcacgac atgcggacgc cggttcgcat cgcattcgcc acgctgctgg tgtcgcaagc
     1201 gatggcggtg atcctgatgt tccagatcgg ccacgccggg ctgacgctgt cgatttcgct
     1261 aggcgcgtgt ctgaacgcgg cgctgctcta ccgcgcgatg cggcggcgcg atgtctaccg
     1321 gccgctaccc ggctggggac gtttcctctg gcgcatcgcc gtcgcgctgt gcgtgctggg
     1381 tgccgtgctg tggtggacgg ccggagacga cgctttctgg ctggcggcgg ggctgtgggc
     1441 gaaggtcggg cgactgggaa tgatcatcgc cgcgggcgcg gtcgcctact tcggcagcct
     1501 ctggctggtt ggctttcgcc tcgcggattt caaccgccgc gagggctagc ctagttgctg
     1561 ccgccggtct tgcggtacgg gcacttcggc cgggtgcatt cggcgtacaa atagagcgcg
     1621 tgctcgctga tctcgaatcc gcgctcgcgg gcaatcttgg tctgccgctt ctcgatctcg
     1681 gcgtcgaaga attcctcgac gcgcccgcat tgcaggcaga ccagatgatc gtggtgcccg
     1741 ccctggttca gctcgaacac cgccttgccg ctttcgaaat gatggcgcac cagcaacccg
     1801 gcctgc
//
LOCUS       DATANS010000607         2286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_451528, whole genome shotgun sequence.
ACCESSION   DATANS010000607 DATANS010000000
VERSION     DATANS010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451528"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VHT22_08920"
     CDS             <1..410
                     /locus_tag="VHT22_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEX3137719.1"
                     /translation="KGARIVEYRGARSSWDVACERPPSDPSDPYHTLLFELADGSVID
                     AGVRGNAARWINHGCEPNCEAIEYDDGRVFIHARRTIRAGEELTYDYRLSFVGPISRR
                     AREALRCHCGAKRCRGTLLLKPPARMKRRARPG"
     gene            complement(417..1616)
                     /locus_tag="VHT22_08925"
     CDS             complement(417..1616)
                     /locus_tag="VHT22_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004543045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3137720.1"
                     /translation="MDFDYSPAVVQWQARLNAFFDEFVYPNEQRYYQEVAAGDRWQPT
                     ELVEELKSAARSAGLGNLFLPASARGAGLTNIEYAPLCEIMGRVLWAPEVFNCSAPDT
                     GNMEVLERYGTPAQKAQWLEPLLAGTIRSAFCMTEPDVASSDATNIESSIMRDGDHYV
                     VNGRKWWSSGAGDPRCRIFIVMGKSDRQAPRHAQQSMLLVPRDAPGVKILRHLPVFGY
                     DDAPHGHMEIEFENVRVPQSSMLLGEGRGFEIAQGRLGPGRIHHCMRLIGLAERALEK
                     MCLRVKSRTTFGKPVAERTVTLERIAESRIMIDQARLLTLKAAYMMDTVGNKAARAEI
                     AMIKVAAPAMACRILDWAIQAHGAAGVCDDFGLAQAYAHARTLRLADGPDEVHRNQIG
                     KLELAKH"
     gene            complement(1649..>2286)
                     /locus_tag="VHT22_08930"
     CDS             complement(1649..>2286)
                     /locus_tag="VHT22_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX3137721.1"
                     /translation="RIDFAAIGLADYGRPGNYLARQIDRWSRQYQASATETIDSMDRL
                     IEWLPRHIPPGERTSLVHGDFRLDNMIFDKHEPRVLAVLDWELSTLGDPLADFAYHLM
                     TWRLAPNEFRGLRGCDFAALGIPTEAEYVAMYLRDNPTLEQPDGQAWDFYMAYNMFRM
                     AGILQGVLRRALAGNAASEQALEAGRRARPLAELAWKQVEERSRAQETRRG"
BASE COUNT          376 a          771 c          728 g          411 t
ORIGIN      
        1 ggaaaggcgc ccgcatcgtc gagtaccgtg gcgctcgctc gagttgggat gtggcttgcg
       61 agcgccctcc gagcgatccc tccgacccct accacacgct gctgttcgag cttgccgatg
      121 gcagcgtgat cgacgcgggc gtgcgtggca acgcggcgcg ctggatcaac cacggctgcg
      181 agccgaactg cgaggcgatc gaatacgacg acggtcgggt cttcattcac gccaggcgta
      241 cgatacgcgc cggagaagag ctgacttacg attaccgcct gagcttcgtc ggcccgatca
      301 gcaggcgggc gcgtgaagcg ctgcgctgcc actgcggcgc gaagcgctgc cgtggcaccc
      361 tgttgctgaa accgccggcc aggatgaaac ggcgcgcgcg gccgggctga tcgcgatcag
      421 tgcttggcga gctccagctt gccgatctgg ttgcgatgca cttcgtcggg tccgtcggcc
      481 aggcgcaagg tccgggcgtg cgcatacgcc tgcgcaaggc cgaagtcgtc gcagacacct
      541 gcggcgccat gcgcctggat cgcccaatcc aggatccggc aagccatcgc gggcgctgcg
      601 accttgatca tcgcgatctc ggccctcgcg gccttgttgc ccactgtatc catcatgtag
      661 gctgccttga gcgtcagcag ccgcgcctgg tcgatcatga tgcgcgactc cgcgatcctc
      721 tccagggtca ccgtgcgttc tgccaccggc ttgccgaacg tcgttcgtga cttgacccgc
      781 aggcacatct tttccagtgc ccgctcggcc agcccgatca ggcgcatgca gtggtgaatg
      841 cggccgggtc ccagccgccc ctgcgcgatc tcgaaccccc tgccctcgcc gagcagcatg
      901 ctggactgcg ggacgcgcac gttctcgaac tcgatttcca tgtggccgtg cggcgcatcg
      961 tcatagccga acaccggcag atgacgcagg attttcacgc cgggtgcgtc gcgcggcacc
     1021 aacagcatcg actgctgcgc gtgacgcggc gcttgtcggt cgctcttgcc catcacgata
     1081 aagatgcgac agcgcggatc gcccgcaccc gacgaccacc acttgcgccc gttgacgaca
     1141 taatggtcgc cgtcgcgcat gatcgagctt tcgatgttgg tcgcatccga cgatgcgacg
     1201 tcgggctcgg tcatgcagaa cgccgagcgg atcgttcccg ccagcagcgg ctcgagccat
     1261 tgcgcctttt gcgcgggcgt gccgtagcgc tcgagcactt ccatgttgcc cgtgtcaggc
     1321 gccgaacaat tgaagacttc gggcgcccag agcacgcgcc ccatgatttc gcacagcggg
     1381 gcgtactcga tgttggtcag gcccgcgccg cgagccgacg ccggaagaaa cagattcccc
     1441 aggccggctg atcgggccgc cgacttgagc tcttcgacca gctccgtcgg ctgccagcga
     1501 tcgccggcgg ccacttcctg gtaatagcgc tgctcattgg gatagacgaa ctcgtcgaag
     1561 aatgcgttga ggcgagcctg ccattggaca accgcaggcg agtaatcgaa gtccatcgtc
     1621 atcctttcgt ggcgcgcatc tcgccgtttc agccgcgccg cgtttcctgc gcgcggcttc
     1681 gctcctcgac ctgtttccac gcgagctcgg ccagcggccg ggcgcgccgc ccggcctcca
     1741 gcgcttgctc gcttgcggcg ttgccagcca gggcgcggcg cagaacgccc tgaagtatgc
     1801 ccgccatgcg aaacatgttg tacgccatgt aaaaatccca ggcctgcccg tccggttgct
     1861 cgagcgtcgg attgtcgcgc agatacatcg cgacgtattc ggcctcggtt ggaatcccga
     1921 gtgccgcgaa atcgcagccg cgcaggccgc gaaactcgtt tggcgcgagc cgccaggtca
     1981 tcaggtgata ggcgaagtcc gccagcggat cacccagtgt agacaattcc cagtcgagca
     2041 ccgccagcac cctgggctcg tgcttgtcga agatcatgtt gtccaggcgg aagtcgccgt
     2101 ggaccagcga cgttcgctcg cccggcggaa tgtgccgcgg cagccattcg atcaggcgat
     2161 ccatcgagtc gatggtttcg gtcgccgacg cctggtattg gcggctccat cggtcgatct
     2221 gccgcgcgag ataattgccg gggcgcccgt aatcggcaag accgatcgcg gcgaagtcga
     2281 tgcgat
//
LOCUS       DATANS010000608         1698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_367611, whole genome shotgun sequence.
ACCESSION   DATANS010000608 DATANS010000000
VERSION     DATANS010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367611"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VHT22_08935"
     CDS             complement(<1..964)
                     /locus_tag="VHT22_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3137722.1"
                     /translation="MGGRAAQAASSAQGTPPEPRSLRDRVGALRNLPPFVRLVWQTSP
                     LLAVGECVLRLARALLPVATLYVGKLIIDEVIALTRLPNPPGTLREWFDAGELNRIGM
                     LLAVEFGLAVLSDVLGRAVSLLDSLQSEQFGNATSLRLMEHAATLDLEDFEDSELQDR
                     LERARRQAAGRMTLMGQLFSQAADMVTIVSFAAGLIVYAPWLIVLLAIALVPAFIGEA
                     HFNAQSYTLNYARTPERRELDYVRQTATSVESAKEVKIFALNAWLIDRYRALATSFFH
                     ANRRIALRRASWGSLLSAIGTIAYYVAYAYIVWRTLHGDFSIGDL"
BASE COUNT          317 a          576 c          561 g          244 t
ORIGIN      
        1 tgagatcgcc gatggaaaaa tcgccgtgca gcgtgcgcca gacgatgtac gcatacgcga
       61 cgtaataggc aatggtcccg atcgcggaga gcaggcttcc ccaactcgcg cgccgcagcg
      121 cgatgcggcg gttggcatgg aagaagctcg tggcgagcgc gcggtagcgg tcgatcagcc
      181 acgcgttcag cgcgaagatc ttcacttcct tcgccgactc gacgctcgtc gccgtctgcc
      241 gcacgtagtc gagctcgcgg cgctccggcg tgcgcgcgta gttgagcgtg tagctctgcg
      301 cgttgaaatg ggcctcgccg atgaacgcgg gcacgagcgc gatggcgagc agcacgatga
      361 gccacggggc gtacacgatg aggcccgccg cgaagctcac gatcgtcacc atgtcggccg
      421 cctgcgagaa cagctgcccc atcagcgtca tgcggccggc cgcctggcgg cgcgcgcgct
      481 ccaggcggtc ctgcaactcg ctgtcctcga agtcctcgag gtcgagcgtg gccgcatgct
      541 ccatcagccg caggctcgtc gcgttgccga attgctcgga ttgcagcgag tcgagcagcg
      601 acaccgcgcg accgagcacg tccgacagca cggccagccc gaactcgacg gcgagcagca
      661 ttccgatccg gttgagctcc ccggcgtcga accactcgcg cagcgtgccg ggcggattgg
      721 gcagccgcgt cagcgcgatc acctcgtcga tgatcagctt gccgacgtag agcgtcgcca
      781 ccggcagcag cgcccgggcc aggcgaagta cacattcgcc gacggccagc aacgggctcg
      841 tctgccagac cagccgcaca aacggcggca gattgcgcag cgcgccgacg cggtctcgca
      901 gcgagcgcgg ttcgggaggg gtcccctgcg ctgaagacgc agcctgggct gcgcgtcccc
      961 ccatcgtgta atgcttgggc atcgggccgt ggaatgtcag gcggttgtcg gcgcgaaacg
     1021 ccgaagcgga cccatgttac gacaacttcg ccatgcaccc ggataggcgc cgccggccag
     1081 cgtcggcact ccgtgacgtg agacgccgac atcgatgtcg gcgtcagtct tacgtcgaat
     1141 catcatttcg ccgattaccg ctatgggcga gccacggcaa aatgcagtca ccgcatagat
     1201 tgttccggag aacatcatga aggtctgggc cccgagactt gcaatagccg ccgtcgcatt
     1261 aggcggcgcg acgctaggct ttgcgcagac cgctcccaac gcgacgcacg actgcagcgg
     1321 catgaccggt acggcgctcg ccacctgtca gcagctgaat cgcaacgtca acgaaccggc
     1381 accgagcggc gccggcaccc cgaatgattg cagcggcctg accggcgcgc ccttggcgag
     1441 ttgccggcgc ctgaacgcag cggaaaccct cgcgccgagc agcgctgccg gccccaacaa
     1501 cgattgcagc ggccagttcg gcgatgcgct gaaagcgtgc cgcacgttga acggtcagcc
     1561 gacggagtac gacgtgccga ccggcgacgc tgcgacgacc ggcagcacag gcagcacgcg
     1621 atagtgagcc acttcgcttt tcgccggcgt cccggcgcga aagtgcgcag cggaaactcg
     1681 gcaaccgaca aggaaacg
//
LOCUS       DATANS010000609         2971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_114428, whole genome shotgun sequence.
ACCESSION   DATANS010000609 DATANS010000000
VERSION     DATANS010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114428"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VHT22_08940"
     CDS             <1..652
                     /locus_tag="VHT22_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX3137723.1"
                     /translation="YFSGLKMTLLLSVTTILLSLVIGAVGALLQGSPSMLLRLPVNAF
                     VVVFRNTPPLVQLYFFYFGVGSLLRSSLASGNQPLVSNVTWAIISLSFFAGAFNVEIF
                     RSGVEAVPKTTLEAAESLGFSRLRAYALIVLPLAFRISLPALNNNLVNLVKTTTLAYA
                     IAVPEMLYVSSQIWSDELNVPVMMNVLLVCYFGLVGALVYVMNRWERAIQVPGFGQ"
     gene            764..1645
                     /locus_tag="VHT22_08945"
     CDS             764..1645
                     /locus_tag="VHT22_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020093112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX3137724.1"
                     /translation="MRAIDHSPGSTDLPVLLPAAARLARRAAAERRTGSPLLSLRAEH
                     GFALFAVFAVGCGIAQAQAGADQPSILATIWKWTPLLLRGFVFNLAISFVAMAVGTAL
                     GVLLGVGLLSPRRPVKRGAWLATNFFRNAPWLVLLFYCMFLLPFQVTILGVTIPLPDW
                     VKATLGLALPVMANVAEILRGAMQSIPTAQWESAASLAFSRRQTLWMIILPQCVKRML
                     PPWMNLYAILTMATVLASIVGVNEMMTLTRQALSAENRPGLLLSFYSYVLLWFFVYCY
                     PIARWTVRLEARYAVNV"
     gene            1654..2406
                     /locus_tag="VHT22_08950"
     CDS             1654..2406
                     /locus_tag="VHT22_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX3137725.1"
                     /translation="MAESDSRPIVSLRDVHKSFGKVEVLKGVSMDVPKGGVVCIIGPS
                     GSGKSTLLRCINALVPIDSGSIRVADFDVHLLNTDKDRLALRREVSIVFQQYNLFPHK
                     TALENVMMAPVYVLREPREQVRERAIALMKKVGLAAKQDAYPGELSGGQQQRVAIARS
                     LAMRPEVMLFDEVTAALDPETVKEVLNTIRELAAEGMTCMLVTHEMRFARELADTVYF
                     TDGGVIVESGPPAELFDRPKVDRTRAFLSQIL"
     gene            2455..>2971
                     /locus_tag="VHT22_08955"
     CDS             2455..>2971
                     /locus_tag="VHT22_08955"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEX3137726.1"
                     /translation="MNTELLFRDDAYLKTTQARVVAVDERGIELDRTIFYPLGGGQLG
                     DTGVLVRASGERIVIADTRKGDDPGSVRHIRAPGMPQPEPGETLSLAIDWPRRHALMR
                     LHTALHVMSCVVVAPVTGGNISPDKGRLDFDIDMSLLDAQAIEQQTNELIARAVPTET
                     VWISDEELDARP"
BASE COUNT          477 a          954 c          960 g          580 t
ORIGIN      
        1 ctatttcagc gggctcaaga tgacgctgct tctgagcgtc acgaccatcc tgctgagcct
       61 cgtcatcggc gcggtcggcg ccttgctgca aggcagtccg tccatgctgc tgcggttgcc
      121 cgtcaatgct ttcgtggtgg tgttccgcaa cacgccgccg ctggtccagc tgtatttctt
      181 ctacttcggt gtcggcagcc tgctccgcag ctcgctcgca tcgggcaacc agccgctggt
      241 cagcaacgtg acatgggcga tcatatctct gtcgtttttt gccggcgcgt tcaacgtcga
      301 aatcttccgc tcgggtgtcg aggcggtgcc gaagaccacg ctcgaagcag ccgaatcgct
      361 gggcttcagc cgcctgcgcg cctacgcgct gatcgttttg ccgctggcgt ttcgcatcag
      421 cctgccggcg ctgaacaaca acctcgtcaa cctggtcaag acgacaacgc ttgcctacgc
      481 gatcgccgtg ccggagatgc tttatgtgtc gagccagatc tggtctgacg agttgaacgt
      541 gccggtgatg atgaacgtgc tgctggtctg ctacttcggg ctggtcggtg cgctcgtcta
      601 cgtgatgaat cgctgggagc gcgccattca agtgccaggg ttcggccaat gagcgaggcg
      661 gaagcttgaa gacccgaagg gcggcgctta agcgagttcg cgtaggccgg cgcgaaaacg
      721 attgttgttc gcgagacggc aaggaggccg gcgagtgagt tcgatgcgcg cgatcgatca
      781 ttcgccgggc tcgaccgatc tgccggtgct tcttccggca gcggcgcgcc tggcgcggcg
      841 cgccgctgcc gagcgccgca ccggctcgcc actgctgtcg cttcgggcgg aacatggttt
      901 cgcgctattc gccgtcttcg ccgtcggctg cggcatcgct caggcgcagg ccggcgccga
      961 ccagccatca atccttgcga cgatctggaa atggaccccg ctgctgctgc gggggttcgt
     1021 gttcaatctc gcgatcagct ttgtcgcgat ggcagtcggc accgcactcg gggtgctgct
     1081 cggagtcggc ctactgtcgc cgcggcgccc ggtcaagcgc ggcgcgtggc tcgccacgaa
     1141 cttctttcgc aacgcgccgt ggctggtgct gctgttctac tgcatgttcc tgctgccgtt
     1201 ccaggtcacg atcttaggtg tcacgattcc gctgcccgac tgggtcaagg cgacgctggg
     1261 cctcgcgctg ccggtgatgg ccaacgtcgc cgagattttg cgcggtgcga tgcagtcgat
     1321 cccgaccgcg cagtgggagt ccgctgcttc gctggccttc agccgccgcc agacgctgtg
     1381 gatgatcatc ctgccgcagt gcgtcaagcg catgctgccg ccctggatga acctgtacgc
     1441 gatcctgacg atggccacgg tcctcgcctc gatcgtcggc gtcaacgaga tgatgacgct
     1501 gacccggcag gcgctttccg ccgagaatcg ccccggcctg ctgctgtcgt tttacagcta
     1561 cgtgctgctc tggtttttcg tctactgcta cccgatcgcg cgctggaccg tccgtctcga
     1621 agcccggtac gcggtcaacg tctgaactcc gtcatggccg aatccgattc ccgcccgatc
     1681 gtctcgctgc gcgacgtcca caagtcgttc ggcaaggtcg aggtgctcaa gggcgtgtcg
     1741 atggacgtgc ccaaaggcgg tgtcgtctgc atcatcggac cgtccggatc cggcaagtcg
     1801 accttgcttc gctgcatcaa cgcgctggtg ccgatcgaca gtggcagcat ccgcgtcgcc
     1861 gatttcgacg tgcatctgct gaacaccgac aaggatcggc ttgcgctgcg ccgcgaagtg
     1921 tcgatcgtat tccagcagta caacctgttc ccgcacaaga ccgcattgga aaacgtgatg
     1981 atggcgccgg tgtacgtgct gagggagccg cgcgagcaag tgcgcgaacg agcgatcgcg
     2041 cttatgaaaa aggtaggcct cgccgccaag caggacgctt atcccggtga gctctccggc
     2101 ggccagcagc agcgcgtggc gatcgcacgc tcactggcga tgcggccgga agtgatgctg
     2161 ttcgacgagg tcacggcggc gctcgatccg gagacggtca aggaagtgct caatacgatc
     2221 cgcgagctcg cggccgaggg catgacctgc atgctggtga cgcacgaaat gcgctttgcg
     2281 cgcgagctag cggacaccgt ctatttcacc gacggcggcg tcatcgtcga atcgggtccg
     2341 ccggcagagc tgttcgatcg cccgaaagtc gatcggacgc gcgcctttct ctcgcagata
     2401 ctctagcgtg cccgcttcgt tggcgttgcg agccgcattc cgagcgttcc ccgcatgaac
     2461 accgagctac tctttcgcga cgatgcgtat ctcaagacca cgcaggcccg agtggtcgcg
     2521 gtcgatgaac gcggcatcga gctcgaccgg acgatctttt atccgttggg cgggggccag
     2581 ctgggcgaca cgggtgtgct ggtccgcgcc agcggcgagc gcatcgtcat cgccgatacg
     2641 cgcaaaggcg acgatccggg cagcgtccgg cacatccgtg cgccgggcat gccgcaacct
     2701 gaaccgggtg aaacgttgag cctcgcaatc gactggccgc ggcgccatgc gctgatgcgg
     2761 ctgcacaccg cgctgcacgt gatgtcgtgc gtcgtggtgg cgccggtcac cggcggcaac
     2821 atctcgcccg acaaaggccg gctcgatttc gacatcgaca tgagcctgct cgacgcgcaa
     2881 gcgatcgagc agcagaccaa cgagctgatc gcgcgcgccg tgccgaccga gacggtctgg
     2941 atcagcgacg aggagctcga tgcgcggccc g
//
LOCUS       DATANS010000610         2791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_409600, whole genome shotgun sequence.
ACCESSION   DATANS010000610 DATANS010000000
VERSION     DATANS010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409600"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VHT22_08960"
     CDS             <1..862
                     /locus_tag="VHT22_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX3137727.1"
                     /translation="PKALRQREGLTFDDVAPSIVAEAGAGLEFTHCDWFSTYRIQHRS
                     AERFRDNRCFLLGDAAHIHSPVGGQGMNTGIGDAVNIAWKLAAVVGGRAEASLLDSYE
                     PERIAFARRLVATTDRAFTGVTSSSATARLVRLRLVPLLFPLLFRLRPVRRLMFRTVS
                     QTSVNYRGSSLSEGRAGAVHGGDRLPWVEPDLPGTGADNFTPLSSLDWQVHVYGEASA
                     DIRAACSQRGLPLHVFAWRPSMRRIGLRRDAVYLVRPDGYIALADSAGSAKALTAYLD
                     VRELKPRTPS"
     gene            complement(840..2207)
                     /locus_tag="VHT22_08965"
     CDS             complement(840..2207)
                     /locus_tag="VHT22_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX3137728.1"
                     /translation="MAIKSINPATGEVFESYDEAAPDAVNKIIGEANDAFLDWRKRPI
                     AERAGPMRKAAAILRAEAHDHAGLMAREMGKPVRDGIAEAQKCATGCDFYADNAERLL
                     AREMIATEARSSFVTFNPLGIVLAVMPWNFPFWQVFRFAAPGLMAGNAGVLKHASNVP
                     GCALAIEDIFRRAGFPANLFRTLMIGNKRVDAVIEHPLVRAVTVTGSGAAGRAVAAKA
                     GAMLKKTVLELGGSDPYLILEDADLELAARVCAKGRLVNSGQSCIAAKRFIVVDAVRA
                     KFEPLFVEKMKAARMGDPLDDQTEIGPQARSDLRDALHAQVEASLAKGARCLLGGKIP
                     EGRGAYYPATVLTDVRPGMAAYEEELFGPVAAVIPVKTEAEAIAVANDSVFGLGGAVI
                     TRDLARGEHIAAELIESGCVFVNESVRSDARLPFGGVKESGYGRELSSYGIREFVNVK
                     TVYVA"
     gene            complement(2336..>2791)
                     /locus_tag="VHT22_08970"
     CDS             complement(2336..>2791)
                     /locus_tag="VHT22_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015275365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX3137729.1"
                     /translation="AMVMTLIACASAFGYVMALMQLPAKITAFFLTISHNREVILMLI
                     NIMLLILGCLMDLAPLLLICTPILLPVVATFGVDPVHFGMIMLLNLGIGLLTPPVGAT
                     LFVGCAVGKVTMEEVMRGIWPFYAVMFAVLMLVTYIPAISLWLPRMLGL"
BASE COUNT          476 a          942 c          885 g          488 t
ORIGIN      
        1 gcccaaggcg ctgcggcagc gcgaggggct cacgttcgac gacgtcgcgc cgtcgatcgt
       61 cgccgaggcg ggcgcagggc tcgagttcac gcactgcgac tggttctcga cgtaccgcat
      121 ccagcaccgc agcgccgagc gctttcgcga caaccgctgc ttcctgctcg gcgacgccgc
      181 gcacatccac agtccggtag gcggacaggg gatgaacacc ggtatcggcg acgctgtgaa
      241 catcgcctgg aagcttgccg ccgtcgtggg cggacgtgcg gaggcgtcgc tgctcgacag
      301 ttacgagccg gagcggatcg cctttgcccg gcggctggtc gcgacgaccg atcgcgcctt
      361 caccggcgtg accagcagca gtgcaacggc ccggttggtg cgactgcggc tcgtgcccct
      421 tttgttcccg ctgctgttcc gattgcgccc agtgcgaagg ctgatgttcc gcacggtctc
      481 gcagacgtcc gtgaactacc gcggaagcag cctgagcgag ggacgcgccg gagccgtgca
      541 tggcggcgat cgtctgccgt gggtcgaacc ggatttgcct ggcaccggtg cggacaattt
      601 cacgccgctt agctcgctcg actggcaggt gcacgtttac ggcgaagcgt ccgccgacat
      661 ccgggccgca tgcagtcagc gaggattgcc gctgcacgtc tttgcatggc gtccgtcgat
      721 gcgtcgaatc ggtctgcggc gcgacgccgt ctatctggtg cggccggacg gctatatcgc
      781 gctggcggat tctgcaggca gcgccaaggc cctaaccgcc tatctcgacg tccgcgagct
      841 caagccacgt acaccgtctt gacgttgacg aactcgcgga tgccgtacga cgaaagctcg
      901 cggccgtacc cgctctcctt gacgccgccg aacggtagac gggcgtccga gcgcaccgac
      961 tcgttgacga aaacgcagcc gctttcgatc agctcggctg cgatgtgctc gccgcgcgcc
     1021 aggtcgcgcg tgatgacggc gccgcccagg ccgaacaccg agtcgttggc gacggcgatc
     1081 gcctcggctt cggtcttgac cgggatgact gccgcgacag gaccgaagag ctcctcttcg
     1141 tacgccgcca tgccgggcct aacgtcggtc aacaccgtcg ccggatagta cgcgccgcgg
     1201 ccctcgggaa tctttcctcc gaggaggcag cgtgcgcctt tggcgaggct cgcctcgacc
     1261 tgggcgtgca atgcatcgcg cagatcgctg cgcgcctggg ggccgatctc ggtctggtcg
     1321 tcgagcgggt cgcccatcct ggcggctttc attttttcga cgaacagcgg ctcgaatttt
     1381 gcgcggaccg cgtcgactac gataaagcgc ttggcggcga tgcagctctg tcccgagttc
     1441 accagccgtc cctttgcgca gaccctggcc gcgagctcga ggtccgcgtc ctcgagaatg
     1501 aggtacgggt cgctgccgcc gagttcgagg accgtcttct tcagcatcgc acccgctttg
     1561 gccgcgaccg ctctcccggc cgcgccgctg ccggtcactg tcaccgcacg caccagcggg
     1621 tgctcgatca ccgcatcgac ccgcttgttg ccgatcatca gcgtccggaa cagattggct
     1681 ggaaatccgg cgcggcgaaa gatgtcttcg atcgccagcg cgcatcccgg gacgttcgag
     1741 gcgtgcttca gcacgcccgc gttcccggcc atcaatcccg gcgccgcgaa tcgaaagacc
     1801 tgccagaacg gaaagttcca cggcatcacc gccaggacga tgccgagcgg attgaaggtg
     1861 acgaagctcg aacgggcctc ggtcgcgatc atctcccgcg ccagcagccg ttcggcattg
     1921 tccgcataga aatcgcatcc ggtggcgcat ttttgcgcct cggcgatgcc gtcgcggacc
     1981 ggcttgccca tctcgcgcgc catcaggccg gcgtgatcgt gcgcctcggc gcgaaggatt
     2041 gcggctgcct tgcgcatcgg tccggcgcgc tcggcgatcg gcctcttgcg ccaatcgaga
     2101 aaggcgtcgt tggcctcgcc gatgatcttg ttcaccgcat cgggcgcagc ctcatcgtag
     2161 gattcgaaaa cttcgccggt tgccgggttg atcgacttga tcgccatgtc tggaactccg
     2221 aacgtcagct gcgctggtag ctgctccgtc ctcgcgcact acgctgagac cgcgcccggc
     2281 gcgagtcgtt ctgatcgccg accgcgcgag ttcgccgacg gcacggcgtc ggcggctaca
     2341 gtcccagcat gcgcggcaac cagagcgata tcgccggaat ataagtgacc agcatcagca
     2401 cggcaaacat taccgcgtag aacggccaga tgccacgcat cacctcttcc atcgtcacct
     2461 tgccgaccgc gcagcccaca aacagtgtcg cacccactgg aggcgtcagc agcccgatgc
     2521 caagattcaa cagcatgatc atgccgaaat ggaccggatc gacgccgaag gtggcgacca
     2581 ccggtagcag gatcggcgtg cagatcagca gcagcggcgc cagatccatc aggcagccca
     2641 ggatcagcag catgatgttg atcagcatca gaatcacttc cctgttgtgc gaaatcgtca
     2701 ggaagaatgc ggtgatcttg gcaggcagct gcatcagcgc catcacatag ccgaacgcgg
     2761 atgcgcaggc gatcagcgtc atcaccatgg c
//
LOCUS       DATANS010000611         2382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_704589, whole genome shotgun sequence.
ACCESSION   DATANS010000611 DATANS010000000
VERSION     DATANS010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704589"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VHT22_08975"
     CDS             <1..690
                     /locus_tag="VHT22_08975"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX3137730.1"
                     /translation="VVARRNAEGAALTVRDFGLRAPATVIGRVRAQSWGSLVDVAAPE
                     GSVSVDLKLPGRHNVVNALGAIAAATAAGASLDAVAQGLAEFRPIAGRLQAKTGRNGA
                     TLIDDSYNANPDSVRAAIAVLVHAAGSKWLALGDMGEVGQQGVAFHREIGECARAAGV
                     DRLLTLGELTPHAVDAFGAGGEHFSSGEELVAAIERDLGPDVTVLVKGSRFMRMERIV
                     AALGGATAAAH"
     gene            690..1841
                     /gene="mraY"
                     /locus_tag="VHT22_08980"
     CDS             690..1841
                     /gene="mraY"
                     /locus_tag="VHT22_08980"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX3137731.1"
                     /translation="MLLALANWIAIDVRAFNVFTYITLRSVLATMTALVISFVIGPRM
                     IRKLTEYKIGQSVRDDGPQTHLIKAGTPTMGGALILVSIIVTTLLWADLRNRFVWVVL
                     LVTLGFGTVGWVDDYRKVVQRNPKGLSAGKKLLLQSLIGLVAAIYLAFSVSAPSTSQF
                     LQLFQAWLASGLSVDLSPKADLIVPFFKTVSYPLGVWGFIILSYCVIVGSSNAVNLTD
                     GLDGLAIMPTVMVGAALGLFAYVAGRADFSTYLFMPHIPGAGELTVVCAAIAGAGLGF
                     LWFNAYPAEVFMGDVGALALGAALGAIAVIVRQEIVLFIMGGVFVVETLSVMIQVASF
                     KLTGRRVFRMAPIHHHYELKGWKENQVVVRFWIITMLLVLFGLSTLKLR"
     gene            1841..>2382
                     /gene="murD"
                     /locus_tag="VHT22_08985"
     CDS             1841..>2382
                     /gene="murD"
                     /locus_tag="VHT22_08985"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310630.1"
                     /note="UDP-N-acetylmuramoylalanine--D-glutamate ligase;
                     involved in peptidoglycan biosynthesis; cytoplasmic;
                     catalyzes the addition of glutamate to the nucleotide
                     precursor UDP-N-acetylmuramoyl-L-alanine during cell wall
                     formation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX3137732.1"
                     /translation="MIKNRDVVVLGLGLTGLSAARWAARHGARVRVADTRAEPPFAAK
                     LAAELPHVPLLRGPITDATLADAATIVISPGLAKNQPPIVAAVARGAELVGDVELFAR
                     ALRPAQRVLAITGSNGKSTVASLCGELARAAGLSVSVVGNIGEPVLDVLDGHEGGAPW
                     PDVFVIELSSFQLETTSSLKS"
BASE COUNT          341 a          781 c          818 g          442 t
ORIGIN      
        1 gtggtcgcga ggcgcaacgc ggagggcgcg gcgctcaccg tgcgcgattt cggcttgcgc
       61 gcgccggcca ctgtcatcgg gcgcgtgcgt gcgcaatcct ggggcagcct ggtcgacgtt
      121 gcggcgcccg aaggatcggt cagcgtcgac ctcaaattac cgggccggca caacgtggtc
      181 aatgcgctgg gagcgatcgc cgctgcgacg gcggccggcg cgagcctgga cgcggtggcg
      241 cagggcctgg ccgagttccg gccgatcgcc ggccgactgc aggccaagac cggtcgcaac
      301 ggtgcgaccc tgatcgacga cagttataac gccaacccgg attcggttcg cgcggccatc
      361 gccgtgctgg tccacgccgc gggctcgaag tggctggcgc tcggagacat gggcgaagtg
      421 ggccagcaag gggtggcctt ccaccgcgag attggtgaat gcgcgcgcgc ggccggggtc
      481 gaccgcctgc tgaccctggg ggaactcacg ccgcacgcgg tcgacgcttt cggggcgggc
      541 ggcgagcatt tttcgagcgg cgaggagctg gtcgcagcca tcgagcgcga cctggggccc
      601 gacgtcacgg tactggtcaa gggctcgcgc ttcatgcgca tggaacgcat cgtcgcggca
      661 ctcggcggcg ccacggcggc cgcgcactga tgctgctcgc gctcgccaac tggatcgcca
      721 tcgacgttcg cgcgttcaat gtcttcacct acatcacgct gcgctcggtg ctcgcgacga
      781 tgaccgcgct ggtgatctcg ttcgtcatcg gcccgaggat gatacgcaaa ctgaccgaat
      841 acaagatcgg ccaatcggtg cgcgacgacg gcccgcagac gcacctgatc aaggccggca
      901 cgccgacgat gggcggcgcg ctgatcctgg tctcgatcat cgttacgacc ctgctgtggg
      961 ccgatcttcg caaccggttc gtctgggtcg tgctgctggt gacgctcggt ttcgggaccg
     1021 tcggctgggt cgacgattac cgcaaggtcg tgcagcgcaa tccgaagggc ctttcggcgg
     1081 ggaaaaagct gctgctacag tcgttgatcg gcctggtcgc cgcgatctat ctcgctttct
     1141 cggtctcggc gccgtcgacc tcgcaattcc tgcagctgtt tcaggcgtgg ctcgcgagcg
     1201 gcctttccgt cgacctgtcg ccgaaagccg atctgatcgt gccctttttc aagaccgtgt
     1261 cgtatccgct cggcgtgtgg ggcttcatca ttctttcgta ctgcgtcatc gtcggctcga
     1321 gcaacgcggt caatctgacc gacggcctcg acggcctggc gatcatgccg acggtgatgg
     1381 tcggcgccgc gttgggcctg ttcgcctacg tcgccggccg cgccgacttc tcgacctacc
     1441 ttttcatgcc gcacattccc ggcgccggcg agctgaccgt cgtctgcgcc gcgatcgccg
     1501 gagcgggcct gggctttctc tggttcaacg cgtatccggc cgaagtgttc atgggcgacg
     1561 tgggtgcgct ggcgctcggc gccgcgctcg gcgcgatcgc ggtgatcgtc aggcaggaga
     1621 tcgtcctgtt catcatgggc ggcgtgttcg tcgtcgagac gctgtcggtc atgatccagg
     1681 tcgcttcgtt caagctgacc ggcaggcgcg tcttccgaat ggcgccgatc catcatcact
     1741 acgaattgaa aggctggaag gagaaccagg tcgttgtgcg tttctggatc atcaccatgc
     1801 tgctggtgct gttcggcctt tcgaccctga agctgcgcta gtgatcaaga atcgcgacgt
     1861 cgtcgtgctg ggcctgggtc tgaccgggct ctcggccgcg cgctgggcag cgcgccacgg
     1921 cgcgcgcgtt cgcgtcgcgg acacccgcgc cgagccgccg tttgcagcga agctcgccgc
     1981 cgagctgccg catgttccgc tgttgcgcgg accaatcacc gacgcgacgc tcgctgacgc
     2041 cgcgacgatc gtgatcagtc cggggctggc gaagaaccag ccgccgatcg tcgccgccgt
     2101 ggcgcgcggc gccgagctgg tcggcgacgt cgagctcttc gcgcgtgcgc tgcggccggc
     2161 gcaacgcgtg ctggcgatca ccggttcgaa cggcaagagc acggtcgcat cgctgtgcgg
     2221 cgagctcgcg cgcgcagcag gcctgtccgt ctccgtcgta ggcaacatcg gcgagccggt
     2281 actcgacgtg cttgacggac acgagggtgg cgcgccgtgg cccgacgtct ttgtcatcga
     2341 gttgtcgagc ttccagcttg aaaccacatc gagcctcaaa tc
//
LOCUS       DATANS010000612         1833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_704638, whole genome shotgun sequence.
ACCESSION   DATANS010000612 DATANS010000000
VERSION     DATANS010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704638"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VHT22_08990"
     CDS             complement(<1..297)
                     /locus_tag="VHT22_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX3137733.1"
                     /translation="MSSALPAYAELHCISNFTFLRGASRPEELVERAAQLGYAALAIT
                     DECSLAGIVRAHIAAKEYQLKLLVGAEFRLADGLKLVLLATDRESYGDLSQLITT"
     gene            complement(316..1743)
                     /locus_tag="VHT22_08995"
     CDS             complement(316..1743)
                     /locus_tag="VHT22_08995"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX3137734.1"
                     /translation="MLWIALDFPCLPLDTFALSSAHTEPWAIADGIEVLVCNAPAQAL
                     GVRSGMRLSAASALSPQLNYRLRDKAAEAATLATLAAWANQFTPNVSLEPPRALLLDI
                     EGSLRCFGGIRPILQTLQRELDAMGYAATMACAPTATAALLLARAGMQKIVNCKRALE
                     AVVASLPLAALDCDAQTAQTLQAVGVKHVAELMALPRDGLARRGGRPLLDTVDRALGR
                     LPAPRQFFVPPAQFSAKLELAAPVAHSEALLFAARRLINQLTGFLAARNGGIQRFTLT
                     LLHERSAATRVDIGLVAPTRDPAHLTLLVRERLGALALTAPVHTLGVEADDILVLSEA
                     NRELFADRAGTQGEWQKLVERLRARLGPQGVQGVAAEAEHRPERASRACEPGAVSAAP
                     ARGPRPLWLLAAPQPLREVGARPHYRETALALVAGPERIESGWWDDDDVKRDYFVAQT
                     PDHATLWIYRDSRAPRGWYLHGVFS"
BASE COUNT          303 a          603 c          650 g          277 t
ORIGIN      
        1 cgtggtgatc agttgcgaca aatcgccgta cgactcgcga tcggtggcga gcagcacgag
       61 tttcaagccg tcggcgagcc ggaattcggc gccgacgagc agtttcagct ggtattcttt
      121 ggccgcaatg tgtgcgcgca ctatccccgc gagcgagcat tcgtcggtga tggcgagcgc
      181 cgcgtaaccg agctgggcgg cgcgctcgac gagttcctca gggcgcgaag ccccgcgcag
      241 aaaagtgaaa ttgctgatgc aatgcagctc ggcgtacgcc ggtagtgcgg aagacatacg
      301 acatcaactg cgcatttaac tgaacacgcc gtgcagatac cagccgcggg gcgcgcggct
      361 atcacggtaa atccataacg tcgcatgatc gggcgtctgc gcgacgaaat agtcgcgttt
      421 gacgtcatcg tcgtcccacc agccggattc gatgcgctcg ggccccgcga ccagggcgag
      481 cgcggtttcg cggtaatgcg ggcgcgcgcc gacttcgcgc aatggttgcg gcgcggcgag
      541 cagccataac ggccgcggcc cgcgcgcggg cgctgcgcta acggcgccgg gctcgcaggc
      601 gcgcgacgcg cgctcgggcc ggtgctcggc ctccgcggcc acgccttgta ccccttgcgg
      661 ccccaaccgc gcgcgcaggc gctcgaccag cttttgccat tcgccttgcg tgccggcgcg
      721 atccgcaaag agttcgcgat tcgcttcgga cagcacgaga atatcgtctg cctccacgcc
      781 cagcgtatgc acgggcgcgg tgagcgcgag cgcacccagc cgctcgcgca cgagcagcgt
      841 gaggtgcgcg ggatcgcgcg tcggcgccac gagcccgatg tcgacgcggg tggcggcgct
      901 gcgctcatgc aggagcgtca gggtgaaacg ctgaatgccg ccgttgcgcg ccgccaggaa
      961 acccgtcaac tgattgatga ggcgccgcgc cgcgaacagc agcgcttcgc tgtgcgcgac
     1021 cggcgccgcg agctcgagtt tggcgctgaa ttgcgcgggc gggacaaaga actggcgcgg
     1081 cgccggcagc cgtcccagcg cgcggtcgac ggtatcgagc aatggccggc cgccgcgccg
     1141 cgcgagcccg tcgcgcggca acgccatgag ttcggccacg tgcttgacgc cgaccgcctg
     1201 cagcgtctgc gccgtctgcg catcgcagtc gagggcggcg agcggcaggc tcgcgacgac
     1261 cgcttcgagc gcgcgcttgc agttgacgat tttctgcatg ccggcccggg cgagcaataa
     1321 ggccgcggtc gcggtcggcg cgcacgccat cgtcgcagca tagcccatgg cgtcgagctc
     1381 ccgctgcagc gtctgcagaa tgggccgaat gccgccgaag cagcgcaggc tgccttcgat
     1441 atcgagcagc agcgcgcgcg gcggctcgag cgaaacgttg ggcgtgaact ggtttgccca
     1501 cgcggcgagc gtcgcgagcg tggccgcttc ggccgcttta tcgcgcaagc gatagttgag
     1561 ctgcggcgaa agcgcgctgg ccgccgacag gcgcatgccg ctgcgcacgc ccagcgcctg
     1621 cgccggcgcg ttgcagacca gcacctcgat gccgtcggcg atggcccacg gttcagtgtg
     1681 cgccgacgat agcgcgaacg tgtcgagcgg caggcagggg aagtcgaggg cgatccacag
     1741 catgatgatt ccagggtttg aatgcgcgtc gggacgtgac ggcaggtgag ttaagagtcc
     1801 ctaacatcct gaaacgcgtg atgcgctgtc acg
//
LOCUS       DATANS010000613         3662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_30342, whole genome shotgun sequence.
ACCESSION   DATANS010000613 DATANS010000000
VERSION     DATANS010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30342"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1787)
                     /locus_tag="VHT22_09000"
     CDS             complement(<1..1787)
                     /locus_tag="VHT22_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HEX3137735.1"
                     /translation="MIALIAVGLLLLAVRFVIFPRVESNPEVVARLLTAQIGQPVEVG
                     SVTTAWDGWNPRLDIGNLRITEPGASVASVTLPDMRLTVAWDSLLFFQLRLKQLSIDE
                     PRLIVRRDTAGMLHVAGMTLDLTEKSGEQGIVEWVLRQPRIVIRDAFIAWRDELRGAP
                     QLTLEHVVLRLENRAGRHRFGLTGAPPASLAAPLDVRGDFTSGSLADWRGASGRFYAR
                     LDYVDVGAWRAWLPPGLPISAGKGAVRMWLEIAAGEPRDMVADAILSGVEGKLASDLP
                     QLSLARLEGRVGWHDDASRREVYARQLAFAAPGETRFDPTDFQLTMHYDAGRPIDGNA
                     SFSRVELAPLRQLGPYLPLPTRWRDDLARFAPRGTLDSGALHWTGDIAAPSAFDASAN
                     FAGLGFAQEGSASGVTGLSGSIAATDHDGAIKLDSRVLAVHWPHLFAQTLALDTAKGQ
                     LRWRRSPEGYAVQVEQLAFANADAAGVVSGEYRTLADGPGVVDGTAQLSRLDATQLHR
                     YVPLAAGAGVREWIRTSLLAGGSSDARLKVSGNLADFPFADGKKGQFTLTAKAQGVTL
                     DYATHWPRLTDLDGEVRVDGVRISVDGRNA"
     gene            1774..3306
                     /gene="glnE"
                     /locus_tag="VHT22_09005"
     CDS             1774..3306
                     /gene="glnE"
                     /locus_tag="VHT22_09005"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEX3137736.1"
                     /translation="MRAINAIARAECSVLRNEVKKAGEELEAARAKGIIGRSGAILIR
                     KGAAAALNSRVDLAGAYEFSRYAQRLRVADPGLCASVEATLDQPFQWPDGALDGLVRG
                     SDPAALAVALRGLRQRVYLHVLLRDLTGRADLNEVCATMTRLAESAIVAAVDAHSNWL
                     AAIHGEPLGADSAAPEKLIVVAMGKLGGGELNVSSDVDLVFVYPEDGNTHGPKVLANQ
                     DFFDRLGRRVVATLAEPSPAGYVFRTDVRLRPYGDAGPLASSFAALEQYLIAQGRTWE
                     RYAWLKARALTGTRGGELEQLVTPFVYRKYLDYDAYEGLRDVHRQIREQGRRRDHANN
                     VKLGPGGIREIEFIVQALQLVRGGREPALRLRGTLPALAAMRVRGLLPAAAAAELFDA
                     YVFLRMVEHRLQYRDDAQTHDLPHDDADRAALAEAMVCSDAAAFDRLLDRHRNNVDRH
                     FDALFSAADGGAERDPLAVVWIAPAPEDAHLAALAAAGYDDPRALIVELARVRTSPRY
                     LQ"
     gene            3340..>3662
                     /locus_tag="VHT22_09010"
     CDS             3340..>3662
                     /locus_tag="VHT22_09010"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamine synthetase
                     adenylyltransferase/deadenyltransferase"
                     /protein_id="HEX3137737.1"
                     /translation="MPQLLRAAAATSAPQAVFERLLALLETISRRRAYLALLVEHPPV
                     LPRLAQLMGASAWAAGYLMQHPMLLDELLDSRALLTEPDWNAWRKELNAQLAAHPDDA
                     ERQMDA"
BASE COUNT          592 a         1232 c         1235 g          603 t
ORIGIN      
        1 gcgttgcggc cgtcgacgct gatgcgtact ccatcgaccc tgacctcgcc gtcgaggtcg
       61 gtcagccgcg gccaatgcgt tgcgtagtcg agggtcacgc cctgtgcctt ggcggtcagc
      121 gtgaactggc cctttttgcc gtcggcgaat ggaaaatcgg cgagattccc cgagaccttg
      181 aggcgcgcat cgctcgagcc gcccgcgagc aggctggtgc gtatccattc gcgcacgccg
      241 gccccggcgg cgagaggcac atagcgatgc agctgcgtcg catcgaggcg cgaaagttgc
      301 gcagttccgt cgaccacgcc cggtccgtcg gcgagcgttc ggtattcgcc gctgaccacg
      361 cccgcggcat ccgcgttggc gaaggccagt tgctcgacct ggaccgcata gccttcgggg
      421 gatctacgcc agcgcagttg tccctttgcg gtgtcgagcg ccagtgtctg cgcaaacaga
      481 tgcggccagt gcacggcaag cacgcgactg tcgagcttga tcgctccgtc gtggtcggtt
      541 gcggcgatgc tgccggagag accggtgact ccggacgccg aaccttcctg cgcgaacccg
      601 agtccggcga agttggccga cgcatcgaat gcgctgggtg ctgcgatgtc accggtccaa
      661 tgcagtgcgc ccgaatcgag cgttccgcgc ggcgcaaatc gcgcgaggtc atcccgccat
      721 cgtgtcggca ggggcaaata ggggccgagc tgccgcaacg gagcgagctc gacgcggctg
      781 aacgaggcgt tgccgtcgat cggtcggcca gcatcgtaat gcatcgtcag ctggaaatcg
      841 gtcgggtcga aacgcgtttc acccggcgca gcgaacgcga gctggcgtgc gtagacttcg
      901 cggcgcgaag catcgtcatg ccagccgaca cggccttcga gccgggccag cgaaagctgt
      961 ggcaaatcgc tggcaagctt gccctcgaca ccgctgagga ttgcgtccgc aaccatgtcg
     1021 cgcggttcgc cggccgcgat ctccagccac attcgaacgg cgcctttgcc agcgctgatc
     1081 ggaagacccg gaggcagcca tgcgcgccag gcaccgacat cgacatagtc gagtcgcgcg
     1141 tagaagcgtc cggacgcgcc gcgccaatcg gccagcgagc cgctggtgaa atcgccgcgc
     1201 acgtcgagcg gtgccgcgag gctcgccggc ggcgctcccg tcaatccgaa gcgatgacgg
     1261 ccggcgcggt tttccagccg cagaacgacg tgctcgagcg tcagctgcgg cgcgccccgc
     1321 agctcgtctc gccaggcgat gaatgcgtcg cggatgacta tgcgcggctg gcgcagcacc
     1381 cattcgacaa tgccctgctc gccggacttt tcggtgagat ccagcgtcat ccccgcgacg
     1441 tgcagcatgc cggcggtgtc gcggcgaacg atcagccgcg gctcgtcgat cgacagctgc
     1501 ttcaggcgca actggaaaaa cagcagtgaa tcccaggcga cggtcagccg catgtcaggc
     1561 aaggtcacgc tcgcgacact ggcacccggc tccgtgatgc gcagattgcc gatgtcgagc
     1621 cgcgggttcc agccgtccca ggcggtagtg accgagccga cttccaccgg ctgaccgatc
     1681 tgggccgtca gcagacgcgc cacaacttcg gggttgctct cgacgcgcgg aaagatgacg
     1741 aagcgcaccg ccagcagcaa caagccgacg gcgatgaggg cgatcaacgc gatcgcgcgt
     1801 gccgagtgca gcgtcttgcg aaacgaagta aagaaggcgg gggaagagct cgaagctgcg
     1861 cgcgcgaagg gcataatagg tcgttctggc gcgattttaa tacggaaagg ggcggccgcc
     1921 gcgttgaatt ccagggtgga ccttgctgga gcgtacgagt tcagccgcta tgcgcaacgc
     1981 cttcgcgtcg ccgatcccgg cctgtgcgcc tccgtggagg cgacgctcga ccagcctttc
     2041 caatggcctg acggcgcact cgacgggctc gtccgcggat ccgatcccgc ggccctggct
     2101 gttgccctgc gtgggctgcg ccagcgcgtg tacctgcacg tcctgctgcg cgacctgacc
     2161 ggacgcgccg atctgaacga agtctgcgcg acgatgacgc gtctggccga gagcgcgatc
     2221 gtcgccgcag tcgacgcgca ctcgaactgg ctcgcggcga tccacggtga gccgctcggt
     2281 gcggatagcg ccgcgcccga gaagttgatc gtcgtggcga tgggcaagct cggcggcggc
     2341 gagctcaatg tctcatccga cgtcgatctc gttttcgtct atccggagga cggcaacacg
     2401 cacggtccga aggttctggc gaaccaggat ttcttcgatc gcttggggcg ccgcgtcgtc
     2461 gccactctgg ccgaaccctc tccggcaggg tatgtattcc gcaccgacgt ccgattgcgt
     2521 ccgtacggtg atgcgggtcc gctggcctct tcgttcgctg cgctcgagca gtacctgatc
     2581 gcgcagggcc gcacctggga gcgctacgcg tggctcaagg cgcgcgcatt gaccggcacg
     2641 cgcggcggcg agctcgagca gctggtcacc cccttcgtct accgaaaata cctcgactac
     2701 gatgcgtatg aaggcttgcg cgacgtacac cgccagattc gcgagcaggg ccggcgccgc
     2761 gatcatgcga acaacgtcaa gctcggaccc ggcggcattc gcgaaatcga attcatcgtc
     2821 caggcgctgc agctggtgcg cggcgggcgc gagccggcgc ttcgtcttcg tggcaccttg
     2881 ccggcgctcg ccgcgatgcg tgtccgtggc ctgctgccgg ccgccgcggc ggccgagctc
     2941 ttcgacgcgt atgtgttcct gcgaatggtc gagcaccgcc tgcagtatcg tgacgatgcg
     3001 caaacacacg acctgccgca cgacgatgcc gatcgcgcgg cgctggccga ggcgatggtg
     3061 tgctccgatg ctgccgcgtt cgatcggctg ctcgatcggc atcgcaataa cgtggaccgc
     3121 cacttcgacg cgcttttttc cgccgccgac ggcggcgccg agcgcgatcc gctcgccgtc
     3181 gtctggatcg cgccggcacc cgaggacgcc catctggccg cactggccgc cgcagggtac
     3241 gacgacccac gtgcgttgat cgtcgagctc gcgcgcgtgc gaaccagtcc gcgctatctg
     3301 caatagccaa cggcttcgcg cgcccgcttc gacgccctgg tgccgcagtt gctgcgcgca
     3361 gcggcggcga cctcggcacc gcaggcggtg ttcgagcgtc tgctcgcact gctggagacg
     3421 atcagccggc gccgcgccta tctcgccctg ctggtcgagc acccgccggt gctgccgcgc
     3481 ctcgcgcagc tgatgggcgc gagcgcgtgg gccgcgggtt acctgatgca gcacccgatg
     3541 ctgctggacg aacttctgga cagccgtgcc cttctgaccg agcccgattg gaatgcgtgg
     3601 cgcaaggagc tcaatgcgca gcttgccgcg cacccggatg atgccgagcg gcagatggat
     3661 gc
//
LOCUS       DATANS010000614         1695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_157375, whole genome shotgun sequence.
ACCESSION   DATANS010000614 DATANS010000000
VERSION     DATANS010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157375"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VHT22_09015"
     CDS             complement(<1..341)
                     /locus_tag="VHT22_09015"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3137738.1"
                     /translation="MLKIYGSVNSRAMRVLWMASELGLAYEHKDWLPRAPETKTPDYR
                     ALNANGRVPTIDDDGFILSESMAINLYLAKKHKSPLYPADLKKEALALQWSLWETDRL
                     DRQVVDYQRHK"
     gene            543..845
                     /locus_tag="VHT22_09020"
     CDS             543..845
                     /locus_tag="VHT22_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HEX3137739.1"
                     /translation="MKLVDIAKVIRSKNAGPTTLTLDLLFNDEAGFKAACASKSLTAD
                     AIAKLYSQPRAKVEVLPYPPALAIKIVMPRRVVSGDPGDSDVYGAQQHGPMLAVEL"
     gene            842..>1695
                     /locus_tag="VHT22_09025"
     CDS             842..>1695
                     /locus_tag="VHT22_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX3137740.1"
                     /translation="MTTAIERIRKSVVGSDGKLAPLRVMAASGQLGYGIPDPAFNAGL
                     ERKPHLIGADMGSIDPGPYYLGAGKMAQSDTMVRVDLAKCINGARKLGVPLLIGTAGS
                     AGAAPHLAATLDMVREIARRDGLHFRLASISADMPRDVVKKAIRAKKVVPLGEIGALT
                     EADVDLATNLVGQMGIEAFQRALNAGADVIIAGRACDSAVFAAVPSLLGYPMGPTLHM
                     SKIIECCSLCCTPGGRDTIIGTLEGDSFTVDSMNPTRNASPMSVAAHSLYEQADPYAV
                     REPEGTLHT"
BASE COUNT          318 a          552 c          519 g          306 t
ORIGIN      
        1 ctcttgtgcc gctgatagtc gacgacctgg cggtcgagcc ggtcggtttc ccacaggctc
       61 cattgcagcg cgagcgcttc cttcttcaga tcggccggat agagcgggct tttgtgcttt
      121 ttcgcgaggt aaaggttgat cgccatcgac tcggacaata tgaagccgtc gtcgtctatg
      181 gtcggcacgc ggccgttggc attcaacgcg cggtaatccg gcgttttggt ttccggcgcg
      241 cgcggcagcc agtccttgtg ttcgtaggcg agcccgagct cggacgccat ccacagcacg
      301 cgcatcgcgc gcgaattcac cgaaccgtaa atcttcaaca tgatcgtgtc ctccagggat
      361 tagtgcaggc ttggatacct gacagggtta cacatcgtgc ggtgcattat gtcaggcact
      421 ctcagtgtag ccgatcccgc tttttcgcgt gtaagatgcg ctctacggag acgcgcacaa
      481 aagaatctcc gccagcctgt cccccacgtt cagccgcact atccacggag agagccgccg
      541 cagtgaaact cgtcgatatc gccaaagtaa ttcgcagcaa aaacgccgga cccaccaccc
      601 tcacgctcga cttgctgttc aacgatgaag cgggcttcaa ggccgcgtgc gcctcgaaat
      661 cattgacggc cgacgcgatc gcgaagctgt actcacagcc gcgcgcgaag gtcgaggtcc
      721 tgccctatcc gcccgcgctc gcgatcaaaa tcgtgatgcc gcgccgcgtc gtttccgggg
      781 atcccggcga cagcgacgtc tacggcgcgc agcaacatgg cccgatgctg gcggtggaac
      841 tatgactacc gcgatcgaac gcatccgcaa gagcgtggtc ggcagcgacg ggaaattggc
      901 gccgctgcgg gtaatggctg catcgggaca actcgggtac ggcatccccg atcccgcttt
      961 caacgccggg ctcgagcgca aaccgcatct gatcggcgcc gacatggggt cgatcgatcc
     1021 cgggccttac tacctcggcg ccggcaagat ggcgcagtcc gacactatgg tgcgcgtcga
     1081 tctcgccaag tgcatcaacg gtgcacgcaa actaggtgtt ccgctgctga tcggcaccgc
     1141 cggcagcgct ggcgcagcgc cacaccttgc agcgacgctc gatatggtgc gcgaaatcgc
     1201 gcgccgcgac ggcctgcatt tccggctcgc gtcgatctcc gccgacatgc cgcgcgacgt
     1261 cgtcaagaaa gccatacgcg cgaagaaagt ggtgccgctc ggcgaaatcg gcgcgctgac
     1321 cgaagccgac gtcgatctcg cgacgaacct ggtcgggcag atgggcatag aagcattcca
     1381 gcgcgcgctg aatgcgggcg ccgacgtgat cattgcgggg cgcgcgtgcg attccgcggt
     1441 gtttgccgcc gttccctcgc tgctcggcta tccgatgggc ccgacgctgc acatgtcgaa
     1501 gatcatcgaa tgctgctcgc tgtgctgcac gccgggcggg cgcgacacca tcatcggcac
     1561 gctcgaaggc gacagcttca ccgtcgacag catgaacccg acgcgcaatg cgtcgccgat
     1621 gtcggttgcc gcgcacagcc tgtacgagca ggccgacccg tatgcagtgc gcgagcccga
     1681 aggcacgctg catac
//
LOCUS       DATANS010000615         2028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_157382, whole genome shotgun sequence.
ACCESSION   DATANS010000615 DATANS010000000
VERSION     DATANS010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157382"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..934
                     /locus_tag="VHT22_09030"
     CDS             170..934
                     /locus_tag="VHT22_09030"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3137741.1"
                     /translation="MRESGIKSTLPLRSAQPLYKQIERSMLQCLANGEWKAGEQLPTE
                     SELAKRFGVAIYTVRAGIGALVHAGILTRKQGKGTFVARHERDRTRQVLTKIFDREHR
                     QIIPTSQKVTFFRKELADERARTLLSLDQRRKSHVYHWEVLVEVDGNAVCVRRMTVPA
                     GLFPGLTGSVLRANRHNLYALYQDLCGVNIIRLEDRVYAIKADAQLARVLGVKHGGPL
                     LKIERVAFTYNEVPVEIRTHFYDSARFHYCADQPGV"
     gene            complement(948..1823)
                     /locus_tag="VHT22_09035"
     CDS             complement(948..1823)
                     /locus_tag="VHT22_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HEX3137742.1"
                     /translation="MKILNIIGGGRAGSTLGRVWARAGTFAIGDVLDRTQELAADAVE
                     FIGSGRAVGAPADMHAADVWMLTPPDDQIIACGKALAASGVLQPNNVVFHISGASPSR
                     ELAAVMTPGARIASVHPLRSFADPALAAQTFSGTYCAAEGDADALTVLRPAFESVGAH
                     VSEIDPAFKTIYHAASVIVSNYLTALMEAGLRSYGKSGLGRGEALRMMEPLVRETVDN
                     VFRLGTVDALTGPIARGDGAVVTRQVDALTAWDPGVAAIYRQLGAIALELARAQAKAD
                     PKALDAIARTLGTAK"
BASE COUNT          371 a          690 c          591 g          376 t
ORIGIN      
        1 cggcccttgc tgcctaccgg cgcctgacga ccgctttttc ctaatttgga ttaagtccgc
       61 cgccttgcat tgacgccccg cacgatgaat tgtgccgtat gcacgtatga cgatttcata
      121 gaaacccact atactttcgc caactcattt ttttccgggc ccgtccgcca tgcgtgaaag
      181 cggaatcaag tcgacgcttc cattgcgctc tgcgcagccg ctgtacaagc agatcgagcg
      241 cagcatgctg cagtgtctcg cgaacggcga gtggaaagcg ggcgagcagc tgccgaccga
      301 atccgaactg gcgaaacgct tcggcgtcgc aatctatacg gtgcgcgccg gtatcggcgc
      361 gctggtccac gccggcatcc tgacgcgcaa gcagggaaag ggcacattcg tcgcgcgcca
      421 tgagcgcgac cgcacgcgcc aggtcttgac caaaatcttc gaccgcgagc atcgccagat
      481 cataccgacc agccaaaagg tgaccttttt cagaaaagag ctcgccgacg agcgtgccag
      541 gaccctcctg agtctcgacc agcgacgcaa atcccacgtc tatcactggg aagtgctggt
      601 cgaagtcgac gggaacgccg tctgtgtccg gcgcatgacc gttcccgccg gcctttttcc
      661 cggcctcacg gggagcgtgc tgcgcgccaa ccggcacaac ctctacgcgc tttatcagga
      721 tctgtgcggc gtcaacatca tccgcctgga ggaccgcgtt tacgcgatca aggccgatgc
      781 gcaattagca cgcgtgctgg gtgtgaaaca cggaggtccg ctgctcaaaa tcgagcgcgt
      841 cgcgtttacc tacaacgaag tcccggtcga aatccgcacc cacttttacg acagcgcgcg
      901 tttccactac tgcgccgatc agccgggcgt ctaaacaaac gaatcattca cttcgcggtg
      961 ccgagcgtgc gtgcgatagc gtcgagcgcc ttcggatcgg ccttcgcctg cgcgcgcgcc
     1021 agttcgagcg cgatcgcgcc gagctgcctg tagatcgccg caacgccggg atcccaggcc
     1081 gtcagcgcat caacctggcg tgtaaccaca gcgccgtcac cgcgcgcgat cgggcccgtc
     1141 agtgcatcga cggtgccgag ccgaaaaacg ttgtcgaccg tttcgcgcac cagcggctcc
     1201 atcatgcgta atgcctcgcc gcgccccagt ccggatttgc cgtagctgcg caggcccgcc
     1261 tccatgagcg cagtcagata attgcttacg atcacgctcg cggcgtggta aatggtcttg
     1321 aacgcggggt cgatttcgga gacgtgcgcg ccgaccgact cgaatgccgg cctgagcacg
     1381 gtgagcgcat cggcatcgcc ttccgcggcg caataggttc cgctgaatgt ttgcgccgcc
     1441 agcgcggggt cggcaaagct cctgagcgga tgcacgctcg ctatgcgcgc gcccggcgtc
     1501 attaccgccg ccagctcgcg cgacggcgat gcaccgctta tatgaaatac tacattgttt
     1561 ggctgcagca ctccgctcgc ggcgagcgct ttgccacagg cgatgatctg gtcgtcgggc
     1621 ggcgtcagca tccacacgtc cgccgcatgc atatcggccg gcgcgccgac tgcgcggccg
     1681 ctgccgatga attccacggc gtcggcggcc agttcctgcg tgcgatcgag cacgtcgccg
     1741 attgcaaacg tgccggcgcg cgcccacacg cgcccgagcg tgctgccagc gcggccgccg
     1801 ccgatgatat ttagtatttt catacgcaac agtgtagcgg gattggcctt tcttgaccgg
     1861 cttctcctcc cgcgcctatg atcgaaccta ccatcgtatt tcgccaggaa atcccgccat
     1921 gaccacagcc gcttcgcccg gcgccgaccg tttcggcaat ccgctcgatc ccatcgtgcg
     1981 ctacgcgcgc ggcactatcc tcgccggcac cgacgaagaa gtcgcgcg
//
LOCUS       DATANS010000616         4786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_576861, whole genome shotgun sequence.
ACCESSION   DATANS010000616 DATANS010000000
VERSION     DATANS010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576861"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VHT22_09040"
     CDS             <1..428
                     /locus_tag="VHT22_09040"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX3137743.1"
                     /translation="EYVGEMSLDGSPRSASVMTVEPTTCAIVNRASVREFLLAHPDFA
                     LNLIEKLIQRVRVTTENVKSLALTDVYGRLVRLITTLAVERDGVMTVPEKLTQQDIAE
                     RVGASRDMISRLLKDLVTGGYLSIADKKITILKKPPTAW"
     gene            742..1053
                     /locus_tag="VHT22_09045"
     CDS             742..1053
                     /locus_tag="VHT22_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137744.1"
                     /translation="MLTLHDSKPIISRVAASLKFAVLLILLGLIVVATERPMLLTPAG
                     AVTVTDDAAYVADQAKRQASTSAAPTARAAAAPAADEFKYFPSQFPAPTGPVEDLPPE
                     F"
     gene            complement(1129..2058)
                     /locus_tag="VHT22_09050"
     CDS             complement(1129..2058)
                     /locus_tag="VHT22_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX3137745.1"
                     /translation="MGLAGFRRGGGSDLVGPAFAMVGVLGFAFKAILIKLAYASTSID
                     AVTLLTLRMAYSAPFFALMAWWSTRAPNAKPLARRDWLSIVWLGFIGYYLSSLVDFIG
                     LQYVTAALERLMLYLYPTIVVVLSAIFFKQRITGRIVVALVLSYVGILLVFARDLELA
                     GDPGALWLGAALVFASSFLYACYLVGVGPVIARLGSLRFIALAMLTSALFIFAHFLAT
                     RPVTALLVPVRIHLLSLTMAVFSTVLPTYLIAEAIKRIGASRTSLLGSMGPLFTIWLG
                     SWLLDEPVHAIQLVGAALVLAGVALVTLRPART"
     gene            complement(2049..2879)
                     /locus_tag="VHT22_09055"
     CDS             complement(2049..2879)
                     /locus_tag="VHT22_09055"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013817163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX3137746.1"
                     /translation="MTAQDLIVAVILGLVEGLTEFLPVSSTGHLIVAGSLLNFTGERA
                     KLFEIVIQAGAIVAVCWEYRVRLFAVARGLPRDRGAQRFVLNLLIAFLPAAVLGLAFA
                     SAIKARLFAPVPVAGAFIAGALVILWAERRQRARPDSVRIRSVDELRWTDALKIGFAQ
                     AFALIPGTSRSGATIIGGMLFGLSRQAATEFSFFLAIPTLFAATGYDLVKNRHLLSTG
                     DLSMIAVGFAVAFVSAFFTIRALLRYVAHHDFVPFAWYRIAFGLVILGTAYAGLVRWD
                     "
     gene            complement(3055..4161)
                     /locus_tag="VHT22_09060"
     CDS             complement(3055..4161)
                     /locus_tag="VHT22_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3137747.1"
                     /translation="MARIDIAVAWLVGERARAAPLLLELLRAIRDLGSIQRASVALGI
                     SYRNGWGLLESWSAELARPLIDKTRGRGTRLTELGELLLRCDDEVRAALDPHCAKLRE
                     RIDRRLGLAHDAPGGLTISASHDLALAAVRDAVNETGVVQLDVQFRGSIDSIDAMIAE
                     RCELAGFHVDGRGGAELVAPYRQRLHAHRHAIIRFAERRQGLLLSPRNPLGIRSLADL
                     PGGPRLVNRQPASGTRLLFDRLLRAAKIDPARITGYDQEEFTHLAVAATIAAGQADVG
                     FGIEAAAAQYRLDFVPLATEQYCLALRRDHLRDVRVVALIDYLASATCRELIGALPGY
                     RASHAGEVIGVDEVLPAASATVVRKGARRGARLA"
     gene            complement(4194..>4786)
                     /locus_tag="VHT22_09065"
     CDS             complement(4194..>4786)
                     /locus_tag="VHT22_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3137748.1"
                     /translation="NLDAKLREEARAWLRELIVRLGLSALVVTHDQTEAMAMSDKILL
                     LNAGRIEQQGTPEELYNRPQTQFTAEFMGSNNHLSGRVAQMADGRALLEGEGWRLWGA
                     ARGALKTGDAATGIIRLERTRIANGAGDNRLRTELVTGMFLGDRKENLFKLGAMRLRC
                     YGTPVPDAGGSDCYLELPQDDFWVFAAPAAAAAPAT"
BASE COUNT          920 a         1603 c         1552 g          711 t
ORIGIN      
        1 gcgaatacgt cggcgaaatg tcgctcgacg gcagtccgcg ctcggcgtcg gtgatgaccg
       61 tcgagcccac gacctgcgcg atcgtcaacc gcgccagcgt gcgcgagttt ctgctggccc
      121 atcccgactt tgcgctgaac ctgatcgaaa agctgatcca gcgggtccgc gtcaccaccg
      181 agaacgtcaa gagcctggca ctgaccgacg tctatggccg gctggtgcgc ttgatcacca
      241 cgctggccgt cgagcgcgac ggcgtgatga ccgtgcccga aaaactcacc cagcaggaca
      301 ttgccgaaag agtcggcgcc tcgcgcgata tgatcagccg cttgctcaag gatctggtga
      361 ccggcggtta tctgagcatc gccgacaaga agatcacgat cctcaagaag ccgccgaccg
      421 cctggtagag tcggcgcgcc gcgcgctttg cgccgcgccg cgcacacaaa acccccgctg
      481 taccgataga ccgcctcgcc atcgcgagct gccggcgccg tcattcggcc cgctacaccc
      541 ggcgactcgc cttcgccggc cgacttattc ggcggctgct gcgtgaattt tgcgcagttt
      601 tttcgacgaa gccgccagcg cggcctcaga cccttgtcga ctacgatttg ctcatccggc
      661 gacgaatggt tcgccgccgg tgaagcggcg aagttcgcag gtgagtgcaa cgccgtcgca
      721 acatcgttca aggagtctgc aatgcttaca ctgcacgatt cgaaaccgat catcagccga
      781 gtcgcggcgt cgttgaagtt tgccgtgctg ctcatccttc tcggccttat cgtcgtggcg
      841 accgagcgtc cgatgttgtt gacaccggcc ggagcggtca ccgtcacaga cgatgcggcc
      901 tacgtggccg atcaggcgaa aaggcaagcg tcgaccagtg ctgcaccgac ggccagggcc
      961 gcggcagcgc ccgcagccga tgaattcaag tattttccgt cgcagtttcc cgcgccgacc
     1021 ggaccggtcg aagatctgcc gccggagttc tgatcgcagc ggacgccgct aaagctgccg
     1081 cctgccgcga catgcccagc gagggcgcgg caggcgcggc gtcagcgact aggttcttgc
     1141 cgggcgcaag gtaaccagcg ccacacccgc gagaaccaat gccgcgccga ccagctggat
     1201 cgcgtgcacc ggctcatcga gcagccacga ccccagccag atcgtaaaca gcggccccat
     1261 cgacccgagc agcgacgtgc ggctggcgcc gatgcgcttg atcgcttccg cgatcaggta
     1321 ggtcggaagc accgtcgaga aaaccgccat cgtcagcgac aagaggtgaa tgcgaacggg
     1381 cacaagcagc gccgtcaccg gacgcgtcgc gaggaaatgg gcaaagatga acagcgccga
     1441 agtcagcatc gccagcgcga tgaagcgaag gctcccgagc cgggcgatca ccggtccgac
     1501 gccgaccaga tagcaggcgt agagaaacga gctcgcgaac accagcgcgg cgccgagcca
     1561 gagcgcgccg ggatcgccgg ccagctcgag atcgcgcgcg aacaccagca ggatcccgac
     1621 atacgacagc accagggcga cgacgatgcg gccggtgatc cgctgcttga aaaaaattgc
     1681 cgacagcacc acgacgatcg tcggataaag gtaaagcatc agccgttcga gggcggcggt
     1741 gacgtattgc aggccgatga agtcgaccag gctcgaaagg taatagccga tgaatcccag
     1801 ccagacgatc gacagccagt cgcggcgcgc gagcggcttc gcgttgggag cgcgcgtcga
     1861 ccaccacgcc atcagcgcga aaaatggcgc tgagtacgcc atgcggagcg tcagcagcgt
     1921 taccgcgtcg atcgatgtcg atgcgtaggc gagcttgatc agtatcgcct tgaaggcgaa
     1981 accgagcacg ccgaccatgg cgaacgccgg accgacgagg tcgctgccgc cgccgcggcg
     2041 aaatccggct aatcccaacg cacgagaccg gcgtacgccg tgccgaggat caccaggccg
     2101 aaggcgatcc ggtaccaggc gaacggaacg aaatcgtgat gcgcgacata acgcagcaga
     2161 gcgcgaatgg tgaagaacgc gctgacgaat gcgacggcga aacccacggc tatcatcgac
     2221 aggtcgccgg tcgacagcag atggcggttc ttgacaaggt cgtagccggt cgcggcgaac
     2281 agtgtcggaa tggcgagaaa aaacgagaac tcggtggcgg cctgccgcga caggccaaac
     2341 agcattccgc cgataatcgt cgccccggag cgcgaagtgc ccggaatcag cgcaaaggcc
     2401 tgcgcgaagc cgatcttcag cgcgtcggtc cagcgcaact catcgacgct gcggatgcgc
     2461 acgctgtcgg gcctcgcccg ctgccgccgc tcggcccaca ggatgaccag cgctcccgcg
     2521 atgaacgcgc cggcgacggg aaccggggcg aacagccgcg ccttgattgc gctggcgaag
     2581 gcgagtccca gcactgcggc gggcaggaac gcgatcagca ggttcagcac aaagcgctgc
     2641 gcgccgcgat cgcgcggcag cccgcgggcg acggcgaaaa gcctgacccg atattcccag
     2701 cagacggcga cgatggcgcc ggcctggatg acgatctcga acagcttcgc ccgctcgccg
     2761 gtgaaattca gcagcgatcc ggcgacgatc aggtggccgg tcgaagacac cggcagaaat
     2821 tcggtcaggc cctcgaccag gccgaggatg actgcgacga tgaggtcctg tgctgtcatt
     2881 gcttatgcgg gtcgcaaaaa aagcgggcgg ctcgcgccgc ccggccgttt atttaaagcc
     2941 tgtgccgccg cgccagatcg ctttgctcag gttgagcgcc ttgccgccgg cgcagccatt
     3001 gcctgtgcct tgctatccac gcgtccttga ttgtacgctc tgccgccgca gcggtcaggc
     3061 caggcgcgca ccgcgccgcg ctcccttgcg cacgaccgtc gcggatgccg caggcaaaac
     3121 ctcgtcgacg ccgatgactt cgccggcatg cgacgcacgg taccccggca gcgcgccgat
     3181 cagctcgcgg caagtcgcgc ttgcgaggta gtcgatcagg gcaacgaccc gaacgtcgcg
     3241 caagtgatcg cgccgcagcg ccaggcagta ctgttcggta gccagcggca cgaaatcgag
     3301 acgatactgg gcggcagcgg cctcgatgcc gaacccgacg tcggcctggc cggcggcgat
     3361 cgtcgccgcg accgcaagat gcgtgaattc ctcctggtcg tagccggtga tgcgggccgg
     3421 gtcgatcttg gcggcgcgca gcaggcgatc gaacaagagg cgcgttcccg acgccggctg
     3481 acggttgacc aggcgcggac cgccggggag gtcggccagt gagcgtatgc ccagcggatt
     3541 ccgcggcgac aacagcaggc cctggcgcct ttcggcaaag cgaatgatcg cgtgccgatg
     3601 cgcatgcagc ctttggcgat aaggcgcaac gagctcggcg ccgccacggc catcgacatg
     3661 gaacccggca agctcgcagc gctcggcgat catcgcgtcg atgctgtcga tactgcctcg
     3721 aaattgcacg tcgagctgga cgacgcccgt ctcgttgacc gcatcgcgaa ccgcggccag
     3781 cgcgagatcg tggctcgcgc tgattgtcag tcctccagga gcgtcgtgcg caagcccgag
     3841 ccggcgatcg atgcgttcgc gcaatttcgc gcaatgcggg tccagcgcgg cgcggacctc
     3901 atcgtcacag cgcaacagca attcgccgag ctcggtaagc cgcgtaccgc gtccacgggt
     3961 tttgtcgatc agcggccggg cgagctcggc gctccagctt tcgagcaggc cccagccgtt
     4021 acggtaggaa atgcccagcg cgacgctggc gcgctggatc gaacccagat cgcgtatcgc
     4081 gcgcagcaac tcgagcaaca gcggagcggc acgcgcgcgc tcgcccacca gccatgccac
     4141 cgcgatgtcg attctggcca ttgcagcatg cgcagggcgc gcaatgcgag ccctcaggtc
     4201 gccggcgccg ccgccgctgc gggcgcagcg aatacccaga aatcgtcctg gggaagctcc
     4261 agatagcagt cgctgccgcc ggcatccggc accggcgttc catagcagcg cagtcgcatc
     4321 gcaccgagct tgaacagatt ctccttgcga tcgccgagga acatgccagt gaccagctcg
     4381 gtgcgcagcc gattgtcgcc cgcgccgttg gcgatccggg tgcgttccag acggatgatg
     4441 ccggtggccg cgtcgcccgt cttgagtgca ccgcgcgcgg cgccccataa acgccagccc
     4501 tcgccttcga gcagcgcgcg accgtcggcc atttgcgcaa ccctgcccga caggtggttg
     4561 ttgctgccca tgaactccgc ggtgaattgc gtctgtggtc ggttgtaaag ctcctctggc
     4621 gtcccctgct gctcgatccg gccggcattg agcagcagga tcttgtccga catcgccatg
     4681 gcctcggtct ggtcgtgcgt gaccaccagc gcggaaagcc ccaggcgcac gatcagctcg
     4741 cgcagccagg cccgcgcttc ctcgcggagc ttcgcgtcga gattgg
//
LOCUS       DATANS010000617         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_114721, whole genome shotgun sequence.
ACCESSION   DATANS010000617 DATANS010000000
VERSION     DATANS010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114721"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VHT22_09070"
     CDS             <1..1021
                     /locus_tag="VHT22_09070"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX3137749.1"
                     /translation="RTGADVIVALVAAYEIQLRLCDVVAASGISGRGWHPGTDMQFSS
                     AALAARLLSDDPRVTAHAIAIAGSHNNTLAQSQRGHIPMMKATAEATIAKGGVEAALL
                     AAAGLTGPAEIFEGAAGWAGTVAGEVDFTALTAPVGERYRILDTCLKPYAAVAGAMAP
                     IRAALDLSTREPLVVADIDNVVVKLHAQAVRKASAPRKNYPRDKETADHSYHYCVAVA
                     LLDGACGPAQFTAERIAAADIRDLIAKTSLEPDEELTALWPKSSGGGVVVTMRDGREL
                     ARLHKYPPGHPQNPLSDAEIADKFLELADGVLTREHAHRVIAEIASFEKCENVGELLS
                     YMIAV"
     gene            1070..2038
                     /locus_tag="VHT22_09075"
     CDS             1070..2038
                     /locus_tag="VHT22_09075"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX3137750.1"
                     /translation="MQSVTRYFVALAAVCVPALGSAQTYPTKSVLMIMPLQAGSSVDV
                     GMRIVTQKMAESMGQQIVIDNQPGAAGLIGTERFMRSTPDGYTLATLNDSILTMIPNL
                     YKKVAYDPLKSFAPVSLVAFNTWLLVNHPSLPVKSARELVALAKARPGEINYASGGQG
                     SPQHIAMAMFEAATGAKMTHVPYKGTTQATLDVISGHIPVMFSATSLVIEQVREGKVR
                     ALAAGGARRLALLPNVPTIAESGVPGFDFNTWMSIFAPLNTPKEVVARLNAEILKALA
                     LPDVKERLVSIGFEIDGSPPEKLAAITKARLEQMQKIIKDAGIVIQ"
     gene            2063..>2735
                     /locus_tag="VHT22_09080"
     CDS             2063..>2735
                     /locus_tag="VHT22_09080"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX3137751.1"
                     /translation="MPTIAVFTKNRTNPAYAAARLGADRTAQRMGARTVHYVPHKPDD
                     AAEQIALIDEALAAGPAACALVPAHPTAVNLAIRKIHAAGVPIVGFVNPFTEPQYIVS
                     FVGSEDYPLAFEMGVYLGEHLQRRGNIVIVEGPPESVSSRERTRGFRDAVKTFPGMRV
                     ATTICGNYQRDNTLHAGDALLRSAVRFDAILAANDVMALAMLEVLAAAGLPRIVTGIN
                     AVPDAI"
BASE COUNT          518 a          837 c          893 g          487 t
ORIGIN      
        1 ccgcaccggc gccgatgtga tcgtggcgct ggtcgccgcg tacgaaattc aactgcgcct
       61 gtgcgacgtc gtcgcggcat ccggcatttc cgggcgcggc tggcatcccg gcaccgatat
      121 gcagttctcg agcgcggcac tcgcggcgcg cctcttgagc gacgatccgc gcgtgaccgc
      181 ccatgcaatc gcgattgccg gcagccacaa caatacgctc gcgcaatccc agcgcgggca
      241 tatcccgatg atgaaagcga ctgccgaggc gaccatcgcg aaaggcggcg tcgaggcggc
      301 gctgctggcc gcggcgggac tcaccgggcc cgccgaaatt ttcgagggtg cggcaggctg
      361 ggcgggcacg gttgcgggcg aagtggattt caccgcgctg acggcaccgg tcggcgagcg
      421 ttatcgcata ctcgatacct gtctcaagcc ttacgcggcg gttgccggcg cgatggcgcc
      481 tatccgcgcc gcgctcgatc tctccacgcg cgagccgctg gtggtggcgg atatcgacaa
      541 cgtagtggtc aagctgcacg ctcaggccgt caggaaagcc tcggctccgc gcaagaacta
      601 tccgcgcgac aaggaaaccg cggaccacag ttaccactat tgcgtggcgg tggcgctgct
      661 cgacggcgca tgcgggcccg cgcagttcac cgccgagcgc atcgccgccg ccgatatccg
      721 tgatctgatt gcgaagacca gtctcgagcc ggatgaggaa ctgaccgcgc tgtggccgaa
      781 atcatccggc ggtggcgtcg tcgtcacgat gcgcgatggc cgcgaactcg cacgcttgca
      841 taaatatccg cccggccatc cccaaaatcc gttgagcgac gccgaaatcg cggacaagtt
      901 tctcgagctg gcggacggag tgctgacgcg cgaacacgcc catcgtgtca tcgctgagat
      961 cgcatcgttc gaaaagtgcg aaaacgtggg agaactgctg tcatatatga tcgcggtatg
     1021 atgccgggcg gcgcaaaata caataaagca aacaagcgag gaggtggtta tgcaatcggt
     1081 cacgcgatat ttcgttgcac tggcggcagt gtgcgtgccg gcgcttggca gcgcgcaaac
     1141 atatcccacg aagtcggtct tgatgatcat gcctttgcag gccggcagct cggtcgacgt
     1201 cggcatgcgc atagtcaccc agaaaatggc cgagagcatg ggtcagcaga tcgtgatcga
     1261 caaccagccg ggggctgcgg gtttgatcgg cacggagcgt ttcatgcggt cgaccccgga
     1321 cggctacacg ctcgcgacgc tcaacgacag catcttgacg atgatcccga acctctacaa
     1381 gaaagtcgcc tacgacccgc tcaagagttt cgcgccggtg agcctggtgg cgttcaatac
     1441 gtggctgctc gtcaaccatc cgtcgctgcc ggtcaaatcg gcgcgcgaac tggtggcgct
     1501 cgcgaaagcg cggcccggcg aaatcaatta cgcgtccggg ggccagggca gcccgcagca
     1561 tatcgcgatg gcgatgttcg aggcggcgac gggcgccaaa atgacgcacg tgccctataa
     1621 aggcacgact caggcgacgc tcgacgtgat cagcgggcat attcccgtga tgttctccgc
     1681 gacgtcgctc gtgatcgagc aagtccgcga gggaaaagtg cgcgcgctgg cggccggcgg
     1741 cgcccggcgg ctggctttgc tgccgaacgt gccgacgatt gccgagtccg gcgtgccggg
     1801 cttcgatttc aacacgtgga tgtcgatatt cgcgccgttg aatacgccaa aggaagtcgt
     1861 cgcgcggctc aatgccgaaa ttctcaaggc gctcgcgctg ccggacgtga aagagcggct
     1921 ggtttcgatc ggttttgaaa tcgacggctc gccgccggaa aaactcgcgg cgatcaccaa
     1981 ggcgcggctc gagcagatgc agaagatcat caaggacgcg ggcatcgtca tccagtgaaa
     2041 ttgctgcggg agtgaagcag tggtgccgac gattgcggtt ttcaccaaga atcgtacgaa
     2101 ccccgcttat gcggcggcac ggctcggcgc cgatcgtacc gcgcagcgta tgggcgcgcg
     2161 caccgtgcac tacgttccgc acaagcccga tgacgcggcg gagcagattg ccctgatcga
     2221 cgaagcgctc gccgcagggc ctgccgcgtg tgcgctggtg cccgcgcatc cgaccgcggt
     2281 taacctggcc attcgtaaga ttcacgccgc cggcgtgccc atcgtgggct tcgtcaatcc
     2341 ctttaccgag ccgcagtaca tcgtgagttt tgtgggctcc gaggattatc cgctcgcgtt
     2401 cgaaatgggt gtttatctgg gcgagcattt gcagcggcgc ggcaatatcg tgatcgtcga
     2461 gggtccgccg gaatccgtca gcagccgtga gcgcacgcgc ggttttcgtg acgcggtcaa
     2521 aacttttccg ggcatgcgtg tggcaacgac aatttgcggc aactaccagc gtgacaatac
     2581 gctgcacgcg ggcgacgcgt tgctgaggtc ggcagtgcgc ttcgatgcaa tattggcggc
     2641 caacgacgtg atggcgcttg caatgctcga agtgctcgct gctgcgggtc tcccccgtat
     2701 cgtgaccggt atcaacgcgg tgccggacgc gattg
//
LOCUS       DATANS010000618         2681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_283601, whole genome shotgun sequence.
ACCESSION   DATANS010000618 DATANS010000000
VERSION     DATANS010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283601"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..111)
                     /locus_tag="VHT22_09085"
     CDS             complement(<1..111)
                     /locus_tag="VHT22_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEX3137752.1"
                     /translation="MSKAFTREGDDDIEDDVEEASPLPAGARNYMTPGGFA"
     gene            274..1248
                     /locus_tag="VHT22_09090"
     CDS             274..1248
                     /locus_tag="VHT22_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX3137753.1"
                     /translation="MTRIPATLALATLAVVALAAFALALGVGSVALDPMTVLRALAGG
                     GDATARTIVQELRLPRALAAFATGGSLAMAGALLQVLLRNPLADPYVLGVSGGAAVGA
                     LAAMLLGAAAWLIPVGAFAGAFVSTVLVFALARGSGAWSPTRLLLTGVIVAAGWGAVV
                     ALLLSLAPEAQLRGMLFWLIGDLSAPPAAWTPLIVLGLAMAIALPFARDLNALSRGDA
                     VAAALGVAIARLPWLIFALAALLTAVAVTTAGAIGFVGLIVPHAVRLVIGNDQRGLLP
                     ASALAGGAMLLIADTLARQLAAPAQLPVGVVTALIGVPVFLILLRRER"
     gene            1245..2018
                     /locus_tag="VHT22_09095"
     CDS             1245..2018
                     /locus_tag="VHT22_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3137754.1"
                     /translation="MSETALLSVRGLTVRIAALTVCRNLDFSVEPGQCWAILGRNGAG
                     KTTLLHTLAGLRPAAAGTIELAGRPLAQWSPRDVARRRAVLPQDDSDAFAATVLETAL
                     VGRHPHLSRWQWESAADLAIARAALAAMDIGAAEARDVRTLSGGERRRVALAALLTQQ
                     PDLLFLDEPSSHLDLAHQLALLDRLAQLARSGSKAVLMVLHDVNLAVRCCDHALLLDG
                     GVAQAGPAADLLTAARLSRLYGVPLRAVETERGSVFAPV"
     gene            complement(2035..>2681)
                     /locus_tag="VHT22_09100"
     CDS             complement(2035..>2681)
                     /locus_tag="VHT22_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3137755.1"
                     /translation="VGDARSLDLERIVGLAPDLAVAWPYVAAAQIERLQALGIPIYRT
                     DPHTPEAIALDIERLGALAGTSDVARSAATRFRSRLAALRQRGHAASAVRVFYEIWHQ
                     PLYTIGGAHLISAAIELCGGENVFRELSLPAPSVSVEAVLAARPSAIIAGTDGAIRPA
                     WLDDWRQWADLPAVRAGNLFVVDANLLHRAGPRFVDGAEALCAVIDRARANGAR"
BASE COUNT          352 a          947 c          949 g          433 t
ORIGIN      
        1 cgcaaacccg cccggcgtca tgtagttccg ggcgcctgcg ggcagcggcg acgcttcctc
       61 gacatcgtct tcgatgtcgt cgtcgccttc gcgggtgaat gccttgctca tcgatgtcct
      121 ggccgcctcg cgctcaagag ccgctgttgc gaggcaaagc gcgtggccgc aacaacggcc
      181 gaaaggaaat tatagtccac ctgccggagg ctataatcgt tgcccttccg actgcgccgg
      241 tcgcggcgcc aagcaatcga gaagctcgcc gcgatgaccc ggattcccgc cacgctggct
      301 ctagccaccc tcgccgtcgt cgcgcttgcg gcatttgcgc tggcgctcgg cgtcggcagc
      361 gtcgccctcg atccgatgac cgtcctgcgc gcgctggccg gcggcgggga tgcaacggca
      421 cgcacgatcg tgcaggagct gcgtctgccg cgcgcgctcg ccgcgtttgc gaccggcggc
      481 tcgctggcga tggccggcgc gctgttgcag gtattgctgc gaaatcccct ggccgacccg
      541 tacgtgctcg gcgtctccgg cggcgcggcc gtcggcgcgc tggcggcaat gctgctgggc
      601 gcggccgcgt ggctcatccc ggttggggcc tttgccggcg cattcgtctc gacggtgctg
      661 gtcttcgcgc tggcccgcgg ctccggcgcc tggtcgccga cgagactatt gctgaccggc
      721 gttatcgtcg cggctggctg gggggcggtg gtcgcgctat tgctgtcgct ggcacccgag
      781 gcgcaactgc ggggcatgct gttctggctg atcggcgacc tgtcagcgcc gcccgccgca
      841 tggacgccgc tgatcgttct cgggctggcg atggccatcg cgctgccctt tgcgcgggac
      901 ttgaatgcgc tctcgcgcgg cgatgcggtc gcggcggcgc tcggtgtagc gatcgcgcgc
      961 ctcccctggt tgatttttgc gctcgcggcg ctgctgaccg ccgtcgcggt gaccaccgcc
     1021 ggcgcaatcg gcttcgtcgg gctgatcgtg ccgcacgcag tgcggctggt catcggcaac
     1081 gatcagcgcg ggttgctgcc cgcctcggcc ctcgccggcg gcgcgatgct gctgatcgcc
     1141 gacacgctgg cgcgccaact cgccgcgccg gcacagctgc cggtcggcgt ggtcaccgca
     1201 ttgatcggcg tgccggtgtt cctgatcctg ctgcggcgcg agcgatgagc gagacggcgc
     1261 tgctctccgt gcgcggactg acggtgcgca tcgccgcgct gaccgtatgc cgcaacctgg
     1321 acttttcggt ggaaccgggg caatgctggg cgattctcgg acgcaacggc gcgggcaaga
     1381 cgacgctatt gcatacgctg gcaggattgc gcccggcggc cgccggcacg atcgagctcg
     1441 ccgggcggcc gctcgcgcaa tggtcgccgc gcgacgtggc gcgacgccgc gcagtgctgc
     1501 cgcaggatga cagcgacgcg ttcgccgcga cggtgctgga aacggcgctg gtcggcaggc
     1561 atccgcatct gtcgcgctgg caatgggaaa gtgctgcgga tctcgccatc gcccgcgccg
     1621 cactggcggc aatggatatc ggcgccgccg aagcccgcga cgtgcgcacg ctctccggcg
     1681 gcgagcgccg ccgcgtcgca ctcgccgcgc tgctgaccca gcagccggac ctgttgtttc
     1741 tcgatgagcc gtcgagccat ctcgacctcg cgcatcagct cgcgttgctc gatcgcctgg
     1801 cccaactggc gcgaagcgga agcaaggcgg tgctgatggt cctgcacgac gtcaacctcg
     1861 cggtgcgctg ctgcgaccac gcgctgttgc tcgatggcgg cgtggcgcag gcgggcccgg
     1921 cggccgatct cttgaccgcg gcacggctgt cgcgcctcta cggcgtgcct ctgcgcgcgg
     1981 tcgagaccga gcgcgggtcg gtgttcgccc ccgtctagcg gacttaccct tcgctcagcg
     2041 agcgccgttg gctcgggcgc ggtcgatgac cgcgcacagc gcttcggcac catcgacaaa
     2101 gcgaggaccg gcgcggtgca gcagattggc atcgacgacg aagagattcc ccgcgcgcac
     2161 ggccggcaga tcggcccact gccgccagtc gtcgagccac gccggacgaa tcgcgccgtc
     2221 ggttccggcg atgatcgccg acggccgcgc cgccaggacc gcttcgacgc tgaccgacgg
     2281 cgccggcaac gaaagctccc ggaagacgtt ttcgccgccg cacaactcga tcgccgcgct
     2341 gatcaggtgt gcgcctccga tcgtatacaa gggctggtgc cagatttcgt agaacacgcg
     2401 aaccgcgctc gcagcgtgcc cgcgctgacg cagcgccgcc agccgcgaac ggaagcgcgt
     2461 cgcggccgaa cgcgcgacgt cggaggtgcc cgcaagcgcg cccagccgct cgatgtcgag
     2521 cgcgatcgcc tccggggtgt gcggatcggt gcgatagatc ggaatgccca gcgcctgcag
     2581 ccgctcgatt tgcgccgcgg ccacgtacgg ccaggcgacg gcgagatcgg gagcaagacc
     2641 gacgatgcgc tcaagatcga gcgagcgcgc gtcgccgacg c
//
LOCUS       DATANS010000619         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_576951, whole genome shotgun sequence.
ACCESSION   DATANS010000619 DATANS010000000
VERSION     DATANS010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576951"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VHT22_09105"
     CDS             <1..489
                     /locus_tag="VHT22_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEX3137756.1"
                     /translation="FRLNTIYPFARQIGVNEKGSEGSEWKVARLVDVAPRGWVEMGKL
                     DGGIAFDKARDVPGPVTIAVALERTVGDRVQRVVVVGNGHFLSNQFLGNGGNLELGVN
                     MVNWLAGDDALITIQPRPARDSSLELGRGAQYVILFGFLVFLPLAFVSTGILVWWRRR
                     KS"
     gene            486..1343
                     /locus_tag="VHT22_09110"
     CDS             486..1343
                     /locus_tag="VHT22_09110"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEX3137757.1"
                     /translation="MKGRLALNLALLAVVIALALFAYYKPAKTAPEHKLSTLKAAEAT
                     SIKVEIAGEPSIALARTGADWKITAPLTARADGTQVQRLLEILEASAKDRFPAQGLAR
                     YDLNEPAARLTINQQAFGFGGFNQMSREQYVLTQDGIYPLSLRYGSLLPKSVHQVISR
                     QVFAADEAPVAFRLADFALTQNDGKWQLTPPAADLGADDLTRWVDEWRLASALAVLPL
                     SNRTAMTRVKVTLKSGTDIFISVLQREPQFVIARSDQPYEYQLSAEAGKRLLAPPAAE
                     PAKTAAAKK"
     gene            1345..>1575
                     /locus_tag="VHT22_09115"
     CDS             1345..>1575
                     /locus_tag="VHT22_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX3137758.1"
                     /translation="MPELPEVETTRRGLAPLVAGATVTGVVVRNRAMRQPITRNLPRI
                     LTGRRIDALERRAKYLLFRAGDGTLIVHLGMSG"
BASE COUNT          282 a          522 c          515 g          256 t
ORIGIN      
        1 ttccggctca acacgattta cccgttcgcg cgccagattg gcgttaacga aaagggcagc
       61 gaaggcagcg agtggaaggt ggcgcgcctg gtcgacgtcg cgccgcgggg ctgggtcgag
      121 atgggcaagt tggacggcgg catcgcgttc gacaaagcgc gcgacgtgcc gggccccgtc
      181 acgatcgcgg tggcgctcga gcgcaccgtg ggtgaccgcg tgcagcgcgt cgtcgtcgtc
      241 ggcaacggac attttttgag caaccagttt ctcggcaacg gcggcaacct ggagctcggc
      301 gtcaacatgg tcaactggct cgcaggagac gatgcgctga tcacgatcca gccgcggccc
      361 gcacgcgaca gcagtttgga gctagggcgc ggcgcgcagt acgtgattct gttcggcttc
      421 ctcgtcttcc tgccgctcgc gttcgtgagt accggcattc tggtgtggtg gcggcgccgt
      481 aaatcatgaa aggccggttg gcgctgaatc ttgcgctgct ggcggtggtc attgcgctcg
      541 cgctgttcgc gtattacaag ccggccaaaa ccgcgcccga gcacaagctg tcgacgctca
      601 aagccgccga ggcgaccagc atcaaggtcg aaatcgccgg cgagccgtcc atagcgctcg
      661 cacgcaccgg cgccgactgg aaaatcacgg cgccgctgac ggcgcgtgcc gacggcacgc
      721 aagtccagcg tctgctggaa atactcgaag cgtcggcgaa ggaccgcttt cccgcgcagg
      781 ggctcgcgcg ctatgacctc aacgagccgg ccgcgcgcct gaccatcaac cagcaggcgt
      841 tcggcttcgg cggcttcaac cagatgagcc gcgagcaata cgtgctcacg caggacggca
      901 tctacccgct ctccttacgc tacggcagct tgctgccgaa gagcgtgcac caggtgataa
      961 gccggcaggt gttcgccgcc gacgaggcgc cggtcgcgtt tcgcctggcg gatttcgcgc
     1021 tcacgcagaa cgacggcaaa tggcagttga cgccgccggc cgccgattta ggcgccgacg
     1081 atctcacgcg ctgggtcgat gaatggcgac tcgcgtccgc gctcgccgtg ttgcccttaa
     1141 gcaaccgcac ggcaatgacg cgggtcaagg tcacgttgaa atccggcacc gacatcttta
     1201 tttcggtgct ccagcgcgaa ccgcagttcg tgatcgcgcg cagcgaccag ccgtacgaat
     1261 accagctgag cgccgaagcc ggcaaacggc tgctcgcacc gcccgccgcc gaacccgcga
     1321 agaccgcagc cgcgaagaaa tagcatgcct gaactgccgg aagtcgaaac gacccggcgc
     1381 ggcctggcgc cgcttgtcgc gggagcgacc gtgaccggcg tcgttgtgcg caaccgcgcg
     1441 atgcgtcaac cgatcacgcg caatctcccg cgcattctca ccggccggcg cattgacgcg
     1501 ctcgagcgcc gcgccaaata tttgctgttc cgcgccggcg acggcacgtt gatcgtgcac
     1561 ctgggcatgt ccggc
//
LOCUS       DATANS010000620         1563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_72263, whole genome shotgun sequence.
ACCESSION   DATANS010000620 DATANS010000000
VERSION     DATANS010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72263"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VHT22_09120"
     CDS             <1..735
                     /locus_tag="VHT22_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX3137759.1"
                     /translation="TAFAVGTLIGAVGIGGILLIPALSAFAGLSIHAAMATALFTFAF
                     TGVTGTIMFQRRGSIDWSVTRPVLLGAVFFAFVGAWVNSLTGPGALALILAGIIIFAG
                     VYTLARWHGMPEPAFAGHPRAQNFLLLGLGAIAGFGSGLTGVGGPALSVPLMVLCGFP
                     ALTSIGTSQVIQIVAAVSGTLANLKYGTIDYGVAATVTLLEIAGVFAGARIVHAVNAD
                     LLRKFVAVLCVAVGAWLMGRELGWFR"
     gene            723..>1563
                     /locus_tag="VHT22_09125"
     CDS             723..>1563
                     /locus_tag="VHT22_09125"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEX3137760.1"
                     /translation="MVQVASEFSAFLAGRCTHTVALAPGIETPRCFSDAAGEHLATRR
                     AAGLFDFSFMYCVEITGAGSGDFLAALQTRAVDTLPSGRIAYTLLLRDDGSVLIDATV
                     WRLERDRYWLFTGRRDDAHYIADFARAYDVALSDRSTLQSVMAIQGPASAAIIERTLR
                     TANVAALPYFGFTQLAFAGAECRVARIGYSGELGYEIVIATAAAAALWSALVDTGNDH
                     GLREGGFAAIDSLRIEAGHLLFTRELAARAAPAELGLMRLIDFYRQEFCGAAAVRAHR
                     WSLP"
BASE COUNT          211 a          521 c          517 g          314 t
ORIGIN      
        1 accgccttcg ccgtcggcac gctgatcggc gccgtcggca tcggcgggat attgctgatc
       61 cccgccctca gcgctttcgc gggactgtcg atccacgccg cgatggcgac cgcgcttttc
      121 acgttcgctt tcaccggtgt gaccggcacc atcatgtttc agcgccgcgg cagcatcgac
      181 tggagcgtca cgcgtccggt gctgctcggg gcggtgtttt tcgcgttcgt cggcgcgtgg
      241 gtcaattcgc tgaccggtcc cggcgcgctc gcgctgatcc tcgccggcat catcatattc
      301 gccggcgtgt atacgctcgc gcgctggcac ggtatgccgg agccggcatt tgcggggcat
      361 ccgcgcgccc agaatttttt gctgctcggg ctgggcgcga ttgccggatt cggctcgggc
      421 ctgaccggcg tcggcggccc cgccctgtcg gtgccgctca tggtgctgtg cggcttcccg
      481 gcattgacct cgatcgggac cagccaggtc atacagatcg tcgccgcggt gtcgggcacg
      541 ctcgcgaatc tcaaatacgg cacgatcgac tacggcgtcg cggccaccgt cacgctgctc
      601 gagattgccg gcgtgtttgc cggcgcgcgc atcgtgcacg cagtcaacgc cgacctgctg
      661 cgtaaattcg tggccgtgct gtgcgtcgcc gtcggcgcgt ggctcatggg acgcgagctc
      721 ggatggttca ggtagcttcg gaattttccg cgttcctcgc cggccgctgc acgcatacgg
      781 ttgcgctcgc ccccggcatc gaaactccgc gctgcttctc cgatgcggcg ggcgagcatc
      841 tcgccacccg ccgtgctgcg ggacttttcg atttttcatt catgtattgc gtggagatca
      901 ccggcgcggg cagcggggat tttctcgccg cgctgcaaac gcgcgcggtc gataccctgc
      961 catccgggcg tatcgcgtac acgttgctgc tgcgcgatga cggcagcgtg ctcattgatg
     1021 ccaccgtatg gcgcctggag cgcgaccgct actggctatt cacgggacgc cgtgacgacg
     1081 cccattacat agccgacttt gcgcgcgctt acgacgtcgc attaagcgac cgctcgacat
     1141 tacaatccgt aatggccatc cagggcccgg ccagcgcggc gataatcgag cgcacgctgc
     1201 gcacggccaa cgtcgccgca ctgccttatt tcggttttac gcagctggcg ttcgccggcg
     1261 ctgaatgccg ggtcgcacgc atcggatata gcggggagct cggctatgaa atcgtcatcg
     1321 cgacggcggc cgccgccgcg ctgtggagcg cgctggtcga cacgggcaac gatcatgggt
     1381 tgcgcgaggg cgggttcgcc gcgatcgatt cgttgcgcat agaagccggg cacctgctgt
     1441 tcacgcgcga actggcggca cgtgctgcgc cggccgaact gggcctgatg cggctcatcg
     1501 atttttatcg gcaggaattc tgcggcgcgg ctgccgtgcg cgcgcaccgc tggagcctgc
     1561 cgg
//
LOCUS       DATANS010000621         1709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_704931, whole genome shotgun sequence.
ACCESSION   DATANS010000621 DATANS010000000
VERSION     DATANS010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704931"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1397)
                     /locus_tag="VHT22_09130"
     CDS             complement(<1..1397)
                     /locus_tag="VHT22_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010171010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX3137761.1"
                     /translation="MQRDPRYDILFEPVRIGPVTAKNRFFQVPHCNGMGYAMPEAHAA
                     MRAAKAEGGWAVVSTEECEIHPTGDISPYVEARLWDDSDIPALALLCEKVHAHGALAA
                     IELTHNGPTSSNLYSREVAIAPSHQPLKYGYPHQARGMTLRDIRDYRRWHRDAALRAK
                     RAGFDIVYIYAAHDLSLAMHFLQQRRNHRTDEYGGSLENRVRLLRELISDTKEAVGDK
                     CAVAVRFATEEFLGVDGVRESEAREVVHLLADLPDLWDVNIAAWHMDSQTSRFADEGY
                     QEPFTGWVKSITSKPVVGVGRFTSPDTMVGQIRRGVLDFIGAARPSIADPFLPRKIEE
                     GRVEDIRECIGCNICVTGDMTSTPIRCTQNPTMGEEWRKGWHPERIAPSRSAKRVLVV
                     GAGPAGLEAARALGQRGYEVHVAEASRELGGRVNFEARLPGLAQWARVRDWRVTQLQK
                     MANVAIYRENDIKAA"
     gene            complement(1413..>1709)
                     /locus_tag="VHT22_09135"
     CDS             complement(1413..>1709)
                     /locus_tag="VHT22_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX3137762.1"
                     /translation="VFTFIILISTSAYLVMYLFCSLAAVKLALRGDMGVQGRKLIWLL
                     AVAMLGALYSAWTLWGAGKEAFVWGMGLFALGLPLYFLMKWWQRRTAAAVVAAS"
BASE COUNT          308 a          586 c          527 g          288 t
ORIGIN      
        1 tcggcggctt tgatgtcgtt ctcgcgatag atcgcgacgt tcgccatctt ctggagctgc
       61 gtgacgcgcc agtcgcgcac ccgcgcccac tgcgccagcc cgggcaggcg cgcttcgaag
      121 ttcactcgtc cgccaagctc acgcgaggcc tcggcgacat gcacttcgta accacgctga
      181 ccgagcgcac gcgctgcttc caatcctgcc ggcccagcgc cgacgacgag cacccgcttc
      241 gcgctcctgc tcggcgcaat gcgctccgga tgccagccct tgcgccactc ctcgcccatc
      301 gtcggattct gcgtgcagcg gatcggcgtg gaggtcatat cgccggtgac gcagatgttg
      361 cagccgatgc actcgcggat gtcttcgacg cgtccttcct cgatcttgcg cggcaggaac
      421 ggatcggcga tcgacggccg tgccgcgccg atgaaatcga gcacgccgcg gcgaatctgg
      481 ccgaccatcg tgtccggcga cgtgaagcga ccgacgccga cgaccggctt gctggtgatg
      541 ctcttgaccc agcccgtgaa cggctcctga tagccctcgt ccgcgaagcg cgaagtctgc
      601 gagtccatgt gccacgcggc gatgttgacg tcccagaggt ccggcaagtc cgcgagcagg
      661 tgcacgacct cgcgcgcttc gctttcgcgc acgccgtcga cgccgagaaa ttcctcggtc
      721 gcgaagcgca ccgcgacggc gcacttgtcg ccgactgctt ctttcgtatc ggagatcagc
      781 tcacgcagca ggcgcacgcg attttccagg gagccgccgt attcgtcggt gcgatgattg
      841 cgtcgctgct gcagaaagtg catcgcgagc gacagatcgt gagccgcata gatgtagacg
      901 atatcgaagc ccgcgcgctt cgcgcgcagc gccgcgtcac gatgccagcg gcgatagtcg
      961 cggatgtcgc gtagcgtcat cccgcgcgcc tgatgcgggt agccgtactt caacggctga
     1021 tgcgatggag cgatcgcgac ctcgcgcgaa tacaagttcg acgacgtcgg tccgttgtgg
     1081 gtcagctcga tcgccgcgag cgcgccgtgc gcgtgcacct tctcgcacag cagcgcgagc
     1141 gcaggaatgt cactgtcgtc ccagagtcgc gcttcgacgt acggcgagat gtcaccggtc
     1201 ggatgaatct cgcactcctc ggtgctgacc accgcccagc cgccttccgc cttcgcagca
     1261 cgcatcgccg cgtgcgcttc cggcattgcg taacccatgc cgttgcagtg aggaacctgg
     1321 aagaagcggt tttttgccgt gaccggaccg atgcggacag gctcgaacag aatgtcgtag
     1381 cgcggatcgc gttgcatgaa tcgaccggaa aatcaggagg cggcgacaac tgcggcggcg
     1441 gtgcgccgct gccaccactt catcaggaaa tacagcggca gccccagggc gaacagaccc
     1501 atgccccaca cgaacgcctc cttgcccgcg ccccacagtg tccacgccga gtacaacgct
     1561 ccgagcatcg ccaccgcgag caaccagatc aacttccgcc cctgcacgcc catgtcgccg
     1621 cgcaatgcga gcttgacggc cgccagcgag cagaacagat acatcacgag ataggcggag
     1681 gtcgagatca ggatgatgaa cgtgaacac
//
LOCUS       DATANS010000622         3120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_238570, whole genome shotgun sequence.
ACCESSION   DATANS010000622 DATANS010000000
VERSION     DATANS010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238570"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VHT22_09140"
     CDS             complement(<1..322)
                     /locus_tag="VHT22_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HEX3137763.1"
                     /translation="MVRRPDLTRIVLSVLLMGILISGSFWILRPFLLSTIWATMIVVA
                     TWPMMLKLQARLGRRVLAVAIMSSAMVLVFVAPLLLAIQTLVENTDTITRWIRELATS
                     PIPAP"
     gene            complement(436..512)
                     /locus_tag="VHT22_09145"
     tRNA            complement(436..512)
                     /locus_tag="VHT22_09145"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(476..478),aa:Pro,seq:tgg)
     gene            complement(582..878)
                     /locus_tag="VHT22_09150"
     CDS             complement(582..878)
                     /locus_tag="VHT22_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX3137764.1"
                     /translation="MVPHPAAHPHVPRPTKSALAKRVNRIDGQVRGIGKMIDEDRYCI
                     DILTQLSAVQSALDSLGLQLLEHHLHGCVQHAVKSGQGDHAIAEAIGVIRRFTK"
     gene            complement(892..1098)
                     /locus_tag="VHT22_09155"
     CDS             complement(892..1098)
                     /locus_tag="VHT22_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX3137765.1"
                     /translation="MTTVTLKVEGMSCGGCVASVTRVLTALPGVSDATVSLQGGTAQV
                     SFDEARTNPAALRAAIESAGYEVA"
     gene            complement(1118..>3120)
                     /locus_tag="VHT22_09160"
     CDS             complement(1118..>3120)
                     /locus_tag="VHT22_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEX3137766.1"
                     /translation="RRDAAAERAADRARKALAYAELRRETWIAAALTLPLLAQMIPML
                     AAGDWLGRAGAHADWLPRWLQLMLATPVQFWSGRRFYLGAWHALRGGGANMDVLIALG
                     TTMAWGLSAVVTLLGLENRHVYFEAGAAVITLVLLGRLLEARARSGMSAALEGLSRLQ
                     PAMARVRRGGATVEVPLADVAIGNRLIVRAGERMPVDGVVREGTSSVDESMVTGESRA
                     VAKTPGSTVFAGTLNQEGLIECEATGVGSATLLAGIVRLVAEAQGSKAPIQRLADRVA
                     GVFVPVVVVIAIATWGFGYALGGDASAALINAVTVLVIACPCALGLATPTAIVVGTGR
                     GAQLGVLIRNAAALEQAGRLSALVVDKTGTLTEGKPVVAGVIALDGATRSQVLELASS
                     VEQGSTHPLANAIRDAATRDGIVPRPLRAFESVPGKGARASVDVDGHDVVVIAGTLAF
                     LAEQGIAAPDAGALHSIGNTLVGVAADGRVVGMIALADRVRPTSAQAIARAKAAGIDV
                     LMLTGDNSQVAATVAAEVGIDDFRAGVLPEDKAATVRALAAQGKVTGMIGDGVNDAPA
                     LAAADVSFAIGTGSDVAVEAADITVVRNDLNAVVDAILLSRATLAKIRQNLFFAFVYN
                     VLGIPLAALGMLNPVFAGAAMAASSLSVLGNAMLLRRWRPKQ"
BASE COUNT          532 a         1082 c         1013 g          493 t
ORIGIN      
        1 ggggcgcggg gatcggcgaa gtggcgagct cgcgtatcca ccgcgttatc gtgtcggtgt
       61 tctcaaccag cgtttggata gccagcagca acggcgcgac gaacacgagc accattgcgc
      121 tggacatgat ggccaccgcc agaacacgcc gccccaatct ggcctggagc ttgagcatca
      181 tgggccacgt cgccacgacg atcatcgtcg cccagattgt cgaaagcagg aaaggccgca
      241 gtatccagaa cgaaccgctg atcagaattc ccatcagcag gaccgagaga acgatgcgcg
      301 ttagatcggg gcgacggacc atcgttgaca cactctttgt cgcggtcggt gcgtagccaa
      361 tccacgaatc ctgccggttc ggcggatccg cgggtcctga agggcacttg cggcggtcga
      421 atcaactgct tgttctggtc ggggtggaga gatttgaact cccgacatct tgctcccaaa
      481 gcaagcgcgc taccgggctg cgctacaccc cgaaagggct ggattatcgc cgttacaggc
      541 attgcgggtc aattcgcact gcaaggcgtt ttcgcgatcc gtcatttagt gaatcgtcga
      601 atcacgccga tcgcctccgc aatcgcatga tctccctgtc ccgatttgac cgcgtgctgc
      661 acgcagccgt gcaaatgatg ctcgagcagc tgcagtccca gcgagtccag cgcggattgg
      721 accgcgctga gctgggtgag gatgtcgatg caatagcggt cctcgtcgat catcttgccg
      781 atgccgcgca cctgaccgtc aatgcggttg actcgcttgg ccagcgcgga tttggtcggc
      841 cgggggacgt gaggatgcgc ggccgggtgt ggcaccactt ggccgggcgg cttacgcgac
      901 ttcatagcca gcgctctcga tcgcggcgcg caaggccgcc ggattcgtgc gggcttcgtc
      961 gaagctcacc tgcgccgttc caccctgcag actgacggtc gcatcggaca cacccggaag
     1021 cgcggtcagc acgcgcgtga cgctggcgac gcaaccgccg cagctcatgc cttcgacctt
     1081 gagcgtaacc gtggtcatcg atctctcctt gttggattca ttgtttgggc cgccagcggc
     1141 gcaacaacat cgcgttgccg agcaccgata gcgagctcgc cgccatcgcc gccccggcga
     1201 acaccggatt caacatgccg agcgcagcga gcggaatgcc cagcacgttg tagacgaacg
     1261 caaagaacag gttctgccgg atcttggcca gggtcgcgcg ggaaagcagt atggcgtcga
     1321 cgacggcgtt cagatcattg cggaccacgg tgatgtcggc ggcctcgacc gcgacgtcgg
     1381 atccggtgcc gatggcgaaa ctgacgtcgg cggcggcgag cgcgggcgca tcgttgacgc
     1441 cgtcgccgat catgccggtt accttgcctt gcgccgccag cgcgcggacc gtcgccgcct
     1501 tgtcctcggg caatacgccg gcgcgaaaat catcgatgcc gacctcggcc gcgaccgtcg
     1561 ccgcgacctg ggaattgtcg ccggtcagca tcagcacatc gatgccggcc gccttcgcgc
     1621 gcgcgatcgc ctgcgccgag gtcggacgaa cgcgatcggc gagcgcgatc attccgacaa
     1681 cccggccatc ggccgcgacg ccgaccagag tgttgccgat actgtgcaac gctccggcat
     1741 ccggtgcggc gatgccctgc tcggccagga aggcgagcgt gcccgcgatc acgacgacgt
     1801 cgtgtccgtc gacatcgacg ctggcgcgcg ctcccttgcc ggggaccgac tcgaacgcgc
     1861 gcagcggccg cggcacaatc ccgtcgcgcg tcgccgcatc gcgtatcgca ttggccagcg
     1921 gatgcgttga tccttgctcg acgctggacg caagctccag cacctgcgaa cgcgtcgctc
     1981 cgtcgagcgc gatcacgccg gcgaccacgg gcttgccttc ggtcaaggtt ccggtcttgt
     2041 cgacgaccag cgcggagagc cgtcccgcct gttcgagcgc ggcggcgttg cggatcagca
     2101 cgccgagctg cgcgccgcgg ccggtgccta cgacgatcgc cgtcggcgtc gccaaaccca
     2161 gcgcgcacgg gcacgcgatg accagcaccg tcacggcgtt gatcagcgcc gcgctcgcat
     2221 cgccgccgag cgcgtagccg aatccccacg tcgcgatcgc gatgacgacc accaccggca
     2281 cgaacacgcc ggcgacgcgg tcggccagac gctggatcgg cgccttcgat ccctgcgctt
     2341 cagcgaccag acgcacgatg cccgcgagca aggtcgcgct gccgacgccg gtcgcctcgc
     2401 attcgatcag accctcctga ttcagcgtgc cggcgaatac ggtcgacccg ggagtcttgg
     2461 cgaccgcacg gctttcgccg gtcaccatgc tctcgtcgac gctcgacgtg ccctcgcgca
     2521 cgacgccgtc gaccggcata cgctcgccgg cgcgcacgat caggcgattg ccgatggcga
     2581 catcggcgag cggcacctct actgtggcgc cgccgcgacg aacgcgtgcc atcgcaggct
     2641 gcaggcgcga cagtccctcc agcgcggccg acatgccgga ccgggcgcgc gcttccagca
     2701 gcctgccgag cagtaccagg gtgattaccg cggcgccggc ttcgaagtac acgtgtcgat
     2761 tctcgagccc gagcagcgtg acgacagcac tcaagcccca cgccatcgtc gtcccgagcg
     2821 cgatcagcac gtccatgttc gcgccgccgc cgcgcagcgc atgccacgcg ccgaggtaaa
     2881 agcggcggcc gctccagaat tgaaccggcg tcgccagcat cagctgcagc cagcgcggca
     2941 gccaatcggc gtgagcaccg gcgcggccca accagtcgcc ggcggcgagc atcggaatca
     3001 tttgcgcgag cagcggcagg gtcagtgcgg cggctatcca ggtttcgcgc cgcagctcgg
     3061 cgtacgcgag ggccttgcgc gcgcgatccg ccgctcgttc ggcggcggcg tcgcggcgca
//
LOCUS       DATANS010000623         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_452070, whole genome shotgun sequence.
ACCESSION   DATANS010000623 DATANS010000000
VERSION     DATANS010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452070"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VHT22_09165"
     CDS             complement(<1..452)
                     /locus_tag="VHT22_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX3137767.1"
                     /translation="MNAVTTVIVGAGHAALAMSRCLAERSIDHVLLERGEVAQSWRTE
                     RWDSLRLLTPNWQSRLPGFSYTGDDPDGYRTLPETIDLIAGYAKSISAPVRTHTIVTS
                     VRSTESGYLVRTNRGDWRCKAVVLASGACSIARVPSFADRVPPSITTLA"
     gene            complement(574..1131)
                     /locus_tag="VHT22_09170"
     CDS             complement(574..1131)
                     /locus_tag="VHT22_09170"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX3137768.1"
                     /translation="MSDSPAANNGVNVQALLDARDALTKAPAAAQFKWRAACEWKNGT
                     HSHSTVESFYGLGAEQNRKATFKFDADHPEVFASEDKGATPVEFVLVGLASCLTAGIA
                     AVAQNRNIQLNSVTATIEGDMDLAGILGIDSEVRNGFKGITVKYKIDADAKPEDIEAL
                     VAQSQKRSAVYDIVTNPTNVTVVVN"
     gene            complement(1303..>2039)
                     /locus_tag="VHT22_09175"
     CDS             complement(1303..>2039)
                     /locus_tag="VHT22_09175"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3137769.1"
                     /translation="QGVLEHVEMVRRHGTTSIRHASDIGILGPTTIIGHAILPDTHSW
                     IRWHTKDDIRLLGEAGCSVAHCPTPFARYGNILESFGDYVAAGVNMTMGTDTTPHNML
                     EEIRKAGTFARIASRHINNVSSGMLLSAATVAGAKALMRDDLGRLAAGAQADIVLVDL
                     AHPDMMPARDPLRSLIFHAADRAVKDVYVAGRKVVADGRVTTLDHAGACARLAEAQDK
                     MMQLTPQRDYLKRSADEIAPLSLPVR"
BASE COUNT          326 a          651 c          665 g          397 t
ORIGIN      
        1 gccagcgttg tgatggacgg cggcacgcga tcggcaaagc tcggcacccg cgcgatgctg
       61 catgcacccg aggccaacac gacggccttg caccgccagt cgccgcggtt tgtgcgcacg
      121 agatatccgc tctccgtgct gcgcaccgag gtgacgatgg tgtgggttcg caccggagcg
      181 gagatggatt tcgcgtagcc cgcgatgagg tcgatggtct ccggcagcgt ccgataaccg
      241 tcggggtcgt cgccggtata gctgaagccc ggcaaccggc tctgccagtt gggcgtgagc
      301 aggcgcagcg agtcccatcg ctcggtgcgc cagctctgcg cgacttcgcc gcgctccagc
      361 aggacgtgat cgatagaacg ctccgcgagg cagcggctca tcgcaagagc cgcgtgcccc
      421 gcaccgacga tgacggtggt gacagcgttc atccggccgt tttcttatgg agcgaccgtg
      481 agcgctactt cctctttgag catgatcttt tccgaaatcc ggtacccatc cccgatcggg
      541 gtcgaggaca tgcttttccg gatcatgctc tgatcagttc accacgaccg tgacgttggt
      601 cggattggtc acgatgtcgt agaccgccga gcgcttctgc gattgcgcga ccagcgcctc
      661 gatgtcctcg ggcttcgcgt cggcatcgat cttgtatttc accgtgatgc ccttgaagcc
      721 gttgcgcacc tcgctgtcga tgccgaggat gccggcgagg tccatgtcac cttcgatggt
      781 tgcggtcacg gaattcaact ggatgttccg gttctgcgcg accgccgcga tgccggcggt
      841 gaggcagctt gccagtccaa ccaggacgaa ttcgaccggt gtcgcgccct tgtcctcgga
      901 cgcgaatact tccgggtgat ccgcatcgaa cttgaaggtg gccttgcggt tctgttccgc
      961 gccgaggccg tagaagcttt cgacggtcga atggctgtgc gtgccgttct tccattcgca
     1021 cgccgcgcgc cacttgaatt gcgcggccgc aggagccttg gtgagagcgt cgcgcgcgtc
     1081 gagcaaagcc tgaacgttga cgccattatt cgctgctgga ctgtcactca taatgtgcct
     1141 ctctgtttga tcgagaaatc aatgcatgcg gtgaattcac ggcgctattg cgctcaggaa
     1201 cgccttggcg tgtcaactca aaagcccgag acaagacgcc gcttgcgcgc tcggcctacc
     1261 ggcgtgcact cccggcaaat tcgggcagtc ggttgcgacc gttcaccgca ccggcaggct
     1321 cagcggcgcg atctcgtcgg cgctgcgctt gagataatcg cgttgcggcg tgagctgcat
     1381 catcttgtcc tgcgcctcgg caagccgcgc gcacgcgccg gcgtgatcga gcgtcgtcac
     1441 gcgcccgtcc gccacgacct tccggccggc gacgtagaca tccttcacgg cgcgatcggc
     1501 ggcatgaaag atgagcgagc ggagcggatc gcgcgccggc atcatgtcgg gatgggcgag
     1561 atcgaccagc acgatgtcgg cctgcgcgcc ggccgcaagc cggcccagat catcgcgcat
     1621 cagcgccttc gcgccggcga cggtcgcggc ggagagcagc atgccgctcg acacgttgtt
     1681 gatgtggcgc gaggcgatgc gcgcgaacgt gccggccttg cggatttcct ccagcatgtt
     1741 gtgcggcgtc gtgtcggtgc ccatcgtcat gttgacgccg gcggcgacgt aatcgccaaa
     1801 actctccaga atgttgccgt agcgcgcgaa cggtgtcggg cagtgcgcca ccgaacaccc
     1861 ggcctcgccg agcaggcgga tgtcgtcctt ggtgtgccag cggatccacg aatgcgtgtc
     1921 gggcaggatc gcgtggccga tgatcgtcgt cggtccgaga atgccgatgt cgctcgcgtg
     1981 ccggatcgac gtggtgccgt ggcgcctcac catctcgacg tgttccagca cgccctgcg
//
LOCUS       DATANS010000624         2749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_114977, whole genome shotgun sequence.
ACCESSION   DATANS010000624 DATANS010000000
VERSION     DATANS010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114977"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VHT22_09180"
     CDS             <1..781
                     /locus_tag="VHT22_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3137770.1"
                     /translation="YFNMEDPVVGGYTPEKIALRRAIGMGYDVNEFIRVILKGRAVPA
                     TSPIPPDIAGYDPLLKTSAQLYDPTAARALLDRFGYKDRDGDGYREMPDGKPLVLERW
                     SAPTSLTRQEDEQWKKNMDAIGLRIEFRKDALQELRKNARLGKMRMRTDGWNADYPDA
                     ENFMQLLYGPNVGQENHARFSLPEFDKLYDAAYRLPDSPERTRLFDRMTELVVAYAPW
                     RLMEHRMEDHLLQPWILNYKPHPVRTALWRYLDIDPAQRPK"
     gene            complement(821..1813)
                     /gene="dusA"
                     /locus_tag="VHT22_09185"
     CDS             complement(821..1813)
                     /gene="dusA"
                     /locus_tag="VHT22_09185"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEX3137771.1"
                     /translation="MNNLSHRLCAAPMMDWTDRHCRYFFRLITPRARLYTEMVTTGAM
                     MFGNVPRHLDFDPAEHPIALQLGGSDPAALAHCARLGEAWGYDEINLNVGCPSERVQT
                     GSFGACLMAEPALVADCVAAMRDAVALPVTVKHRTGIDAVDEYAFVRDFVGTVAAAGA
                     SAFIVHARNAILKGLSPKENREIPPLKYQFVHRLKRDFPELTIVINGGIATLGDIARE
                     LREVDGVMLGRAAYHHPYVLADADALCYGPGARPRSRADIIAALLPYIEGQLARGVHL
                     RAITRHILGLYHGSPGARTWRRTLSDAALLHDGGPELLLKALRQVEPESVAAAV"
     gene            complement(1841..>2749)
                     /locus_tag="VHT22_09190"
     CDS             complement(1841..>2749)
                     /locus_tag="VHT22_09190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3137772.1"
                     /translation="MRGAWTKDDYATMTRDAQALQRDLGMAVDVLSKAEVRREMAADC
                     YQGGVLFHAHGGVHPALLHRGLLQRARTAGALVAGRTPVTAVRREAARFIVKTPSGSI
                     DATAVVAATNGYTGGTTPALARRLVAIPSFMIATESLGTDRVKSLIPNGRMIVETRDK
                     HLYYRPSPDGTRIVLGGRAALHPIQLDEAAEWLMNELRAIFPTLADTRVSHVWTGNVA
                     MTRSDLPGIGQRDGIWFALGCNGSGVALMPYLGHKVALKVLGRQDGKTAFDDMSFAAI
                     PFYDGTAWFRPLMTWWFRARDRVRSN"
BASE COUNT          501 a          901 c          902 g          445 t
ORIGIN      
        1 ctatttcaac atggaagatc cggtggtcgg cggctacacg ccggagaaga tcgcgttgcg
       61 gcgggcaatc ggcatgggct acgacgtgaa cgagttcatc cgcgtcatcc tcaaaggccg
      121 cgccgttccg gcgacaagcc cgattccacc cgacatcgcc ggctacgacc cgctgctcaa
      181 gacgagcgcg cagctctacg accccacggc agcgcgcgcg ctgctggacc gattcggcta
      241 caaggatcgc gacggcgacg gctaccgcga aatgccggac ggcaagccgc tggtcctcga
      301 gcgctggtcg gcgccgacgt cactgacgcg acaagaggac gagcaatgga agaagaacat
      361 ggatgcgatc ggcttgagga tcgagttcag gaaggatgcc ctgcaggagc ttcgcaagaa
      421 cgcacgactg ggaaagatgc gaatgcgcac cgatggctgg aacgccgatt accccgacgc
      481 cgaaaacttc atgcagctgc tttacgggcc gaacgtcgga caggaaaacc acgcccggtt
      541 cagcttgccg gaattcgaca agctgtacga cgccgcatat cgcttgccgg attcgccgga
      601 acgcacgcgg ctgttcgacc ggatgaccga actggttgtc gcctacgcgc cctggcggct
      661 gatggagcac cggatggagg accatctgct gcagccctgg atcctgaatt acaagccgca
      721 tccggtgcga acggcgctgt ggcgctacct cgatatcgat ccggcgcagc gtccgaaata
      781 aagcgccgct cttccgccga agccggaatc agcgcttggg ctagaccgcg gcggcgaccg
      841 attccggctc gacctggcgt aacgccttga gcagaagctc gggcccgccg tcgtgcagca
      901 gcgctgcgtc ggagagtgtg cgccgccagg tgcgcgcgcc gggagatccg tggtagagcc
      961 cgaggatgtg ccgcgtgatc gcacgcaaat gcacaccgcg cgcgagctgc ccctcgatat
     1021 agggcagcag cgcggcgatg atgtcggcgc gcgagcgcgg ccgggcaccg gggccgtagc
     1081 agagcgcgtc ggcgtcggcg agcacgtacg gatggtgata agcggcgcgc ccgagcatca
     1141 cgccgtcgac ctctcgcagc tcgcgcgcga tatcgccgag cgtcgcgatc ccgccgttga
     1201 tgacgatcgt gagctcggga aagtcgcgct ttagccggtg cacgaattgg tatttcagcg
     1261 gcgggatctc gcgattttcc ttcggcgaca ggcctttgag gatcgcgttc cgggcgtgga
     1321 cgatgaacgc gctggcgcct gccgccgcga cggtgccgac gaagtcgcgc acgaaggcgt
     1381 actcgtcgac cgcatcgatg ccggtgcggt gcttgaccgt caccggcagc gcgacggcat
     1441 cgcgcatcgc cgcgacgcag tcggcgacca gcgcgggttc ggccatcagg caggcgccga
     1501 agctgccggt ctgcacgcgc tcgctgggac agccgacgtt caggttgatc tcgtcgtagc
     1561 cccaggcttc gcccagccgc gcgcaatgcg cgagcgcggc ggggtcgctg ccgccgagct
     1621 gcaatgcgat cgggtgctcg gccggatcga agtcgagatg ccgcggcacg ttgccgaaca
     1681 tcatcgcgcc ggtcgtcacc atttcggtgt acaagcgggc gcgcggcgtg atcagccgga
     1741 aaaagtagcg gcagtgacgg tcggtccagt ccatcatcgg ggccgcgcat aaacgatgcg
     1801 acaagttatt cattccttga ggttctcgac attcgcccgg tcagttactg cgcacccggt
     1861 cccgagcccg gaaccaccat gtcatcagtg gccggaacca ggcggtgccg tcgtagaacg
     1921 gaatcgccgc aaaggacatg tcatcaaacg ccgtctttcc atcctgccgg ccaagcactt
     1981 tcagcgcgac cttgtgcccg agatagggca tcagcgcgac ccccgaccca ttgcagccga
     2041 gcgcgaacca gatgccgtcg cgctggccga tcccgggcaa atcgctgcgc gtcattgcga
     2101 cattgccggt ccagacatgg ctgacgcggg tgtcagcaag cgtggggaaa attgcgcgca
     2161 attcgttcat cagccattcg gcggcctcgt caagttgaat cggatgcaac gcggctcgac
     2221 ccccgagcac gatgcgcgtg ccgtcgggtg acgggcgata gtacagatgc ttgtcccggg
     2281 tctcgacgat catccggcca ttcgggatga ggctcttgac gcgatccgtc cccagcgatt
     2341 cggtggcgat catgaagctc ggtatcgcga cgaggcggcg ggcgagtgcc ggcgtcgtcc
     2401 cgcccgtata accattggtg gcggcaacaa ccgcggtcgc gtcgatgctt ccgctcggcg
     2461 tcttcacgat gaatcgggcc gcttcgcgcc tgaccgcggt cactggcgtg cggccagcga
     2521 cgagcgctcc ggcggtccgc gcccgttgca gcaacccccg atgtaacaac gccggatgca
     2581 cgccgccgtg cgcgtgaaac agcacgccgc cttgatagca atccgccgcc atctcgcggc
     2641 ggacctcggc cttgctcaac acgtcaaccg ccatgccgag atcgcgctgc agcgcctgcg
     2701 catcgcgcgt catcgtcgca taatcgtctt tcgtccatgc gccgcgcat
//
LOCUS       DATANS010000625         2382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_452179, whole genome shotgun sequence.
ACCESSION   DATANS010000625 DATANS010000000
VERSION     DATANS010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452179"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VHT22_09195"
     CDS             complement(<1..306)
                     /locus_tag="VHT22_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA tRNA nucleotidyl
                     transferase/2'3'-cyclic
                     phosphodiesterase/2'nucleotidase/phosphatase"
                     /protein_id="HEX3137773.1"
                     /translation="MKAYRVGGSVRDELLGRTVADRDFVVVGATPEQMVAAGYRPVGS
                     DFPVFLHPQTNDEYALARTERKSGRGYRGFVFHTTPDVTLEADLARRDLTINAIARDD
                     "
     gene            complement(311..1252)
                     /locus_tag="VHT22_09200"
     CDS             complement(311..1252)
                     /locus_tag="VHT22_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEX3137774.1"
                     /translation="MNGKAIVVLGGSGFVGRHLVSRLVADGNRVVVPTRSRERARHLI
                     LLPTVDVVEADVHDAATLARLVRGAAAAINLVGILNESRPGDFDRVHVELPRKLIAAC
                     RDGAVTRLLHMSALNADPQGPSRYLRSKGEAEALVAASGLQWTIFQPSIIVGREDSFL
                     NLFAKLQQLLPVIALACAAARFQPVAIDDVANAYVRALGDDATHGQRYPLCGPKVYTL
                     RELVAYAGELTGYRRPIVALGPGLSKLQASVLERLPGKLMSRDNLASMQRDSVCDCPF
                     PPLFGGPPVALEAIAPTYIAAAAPTGRYGRIRSHRGA"
     gene            complement(1249..>2382)
                     /locus_tag="VHT22_09205"
     CDS             complement(1249..>2382)
                     /locus_tag="VHT22_09205"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX3137775.1"
                     /translation="VAYSAARQLLPEANAWYREVGEAPLNEVQREWRARAALRALAWH
                     DVARAIDAMPESQAQEPAWRYWKARAFEAQSRKDDAIRLFGSLAAEPHFYGFLAADAI
                     GASIMPVSEPLPIDDEALAAFGARPAVQRVLRLATLGMRPEAQREWTYVVRGIDDRSL
                     LLASEFARRNGLYDRSINTADRTQRRHDFGLRYQTPYRDAIGSAVREMGLDEAWVFGL
                     TRQESRFAADVVSSAGAIGLMQLMAPTARWIAQQTHQSDYRITQLGEPEINARFGTYY
                     LRHVLDRLDGLPVLATAAYNAGPGRAQSWRGAAPLEGAIYAETIPFNETRDYVKKVMA
                     NAMFYQAQLGLPYVTLTDRLGIVTPRGTTAGSPVASVDRSALP"
BASE COUNT          395 a          798 c          816 g          373 t
ORIGIN      
        1 gtcgtcgcgc gcgatcgcgt tgatcgtcag gtcgcgtcgc gcaagatccg cttcgagcgt
       61 cacgtcgggc gtcgtatgaa agacgaagcc ccgatagccg cggccgctct tgcgctcggt
      121 ccgcgccagt gcgtactcat cgttcgtctg cggatgcagg aataccggaa agtcgctgcc
      181 gaccggacgg tagcccgcgg ccaccatctg ttcgggcgtg gcgccgacga cgacgaaatc
      241 gcgatcggcg accgtacggc cgagaagctc gtcgcggacc gagccgccca cacgataagc
      301 cttcacgatt tcaggcgccg cgatgcgagc ggatgcggcc gtagcggcct gtcggcgcgg
      361 cggccgcaat gtacgtcggc gcgatcgcct cgagcgcgac gggaggcccg ccgaacaacg
      421 gcggaaacgg gcagtcgcag acactgtcgc gctgcatcga agccagattg tcgcggctca
      481 tcagcttgcc aggaagccgc tcgagcacgc ttgcctggag ctttgagagg ccgggcccga
      541 gcgcgacgat cggccgcctg tagccggtca gctcgcccgc ataggcaacc agttcgcgca
      601 gcgtgtagac cttcgggccg cacagtggat agcgctggcc gtgagtcgcg tcgtcgccga
      661 gtgcgcgcac ataggcgttc gcgacgtcgt cgatcgccac cggctggaag cgcgccgcgg
      721 cgcatgccag cgcgatcacc ggaagcagtt gttgcagctt ggcgaacagg ttgagaaacg
      781 aatcctcgcg cccgacgatg atcgacggct ggaaaatcgt ccattgcagg ccggatgcgg
      841 cgaccagcgc ttcggcctca cccttgctgc gcaggtaccg gctcggcccc tgcgggtccg
      901 cattgagcgc gctcatgtgc agcaggcgtg tgacggcgcc gtcgcggcaa gcggcgatca
      961 gcttgcgcgg caattcgacg tgaacccgat cgaagtcgcc ggggcgcgat tcgttgagga
     1021 tgcccaccag attgatcgcc gccgcagcgc cgcgaaccag gcgagcgagc gtcgccgcgt
     1081 cgtgcacatc ggcttcgacc acgtcgaccg tcggcaaaag gatcagatgt cttgcgcgct
     1141 cgcggctccg ggtggggacg accacgcgat tgccgtcggc aaccagccgc gagacaaggt
     1201 gccgcccgac aaagccgctg ccgccgagaa cgacgatagc cttgccgttc atggcagcgc
     1261 tgagcggtcg accgaagcga ccggcgagcc cgccgtcgtg ccgcgcggcg tgacgatgcc
     1321 gagccgatcg gtcagcgtga cgtaaggcag gccgagttgc gcctgataga acatcgcgtt
     1381 ggccatcact ttcttgacat agtcgcgcgt ctcgttgaac gggatcgtct cggcgtagat
     1441 cgccccttcc agcggcgcgg caccgcgcca gctctgcgca cgcccaggcc ccgcgttgta
     1501 cgccgcggtc gcgagcaccg gcagtccgtc gaggcggtcg agcacatggc gaagatagta
     1561 ggtgccgaag cgggcattga tctcgggctc tccgagctgc gtgatgcgat agtcgctctg
     1621 atgggtttgc tgggcgatcc agcgtgcggt cggcgccatc agctgcataa gtccgatcgc
     1681 gcctgcgctg gagacgacgt cggcggcgaa gcggctttcc tgccgtgtga gcccgaacac
     1741 ccacgcctcg tcgaggccca tttcgcgcac ggcggaaccg atggcatcgc ggtaaggcgt
     1801 ctggtagcgg aggccgaaat cgtgtcgtcg ttgcgtccgg tccgcggtgt tgatcgagcg
     1861 atcgtacaag ccatttcggc gcgcgaactc gctggcgagc aagagcgacc ggtcgtcgat
     1921 gccgcgcacg acgtaagtcc actcgcgctg cgcctcgggc cgcatgccta gcgtggcaag
     1981 acgaagcacg cgctgcacgg cgggacgggc gccaaatgcg gccagcgcct cgtcgtcgat
     2041 cggcagcggc tcgctgaccg gcatgatcga agctccgatc gcgtcggcag cgagaaaccc
     2101 gtagaaatgc ggctcggcgg ccaggctgcc gaacaaccgg atcgcatcgt ccttgcgcga
     2161 ttgcgcctcg aacgctcgcg ccttccagta gcgccacgcc ggctcctgcg cctggctttc
     2221 cggcatcgca tcgatcgcgc gagcgacatc gtgccaggcc agcgcgcgca gcgccgcgcg
     2281 cgcacgccac tcgcgctgga cctcgttcag cggagcctcg ccgacttcgc gataccaggc
     2341 gttggcctcg ggcagcagtt gcctggctgc gctatatgcg ac
//
LOCUS       DATANS010000626         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_705117, whole genome shotgun sequence.
ACCESSION   DATANS010000626 DATANS010000000
VERSION     DATANS010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705117"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VHT22_09210"
     CDS             complement(<1..370)
                     /locus_tag="VHT22_09210"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX3137776.1"
                     /translation="MLTVESGDIVVLESAGAIAPDAVDKSGVVPPSAVPEYVRAIARD
                     VKDRGPGGHILTGPIAVNGALPGDVLEVRVLAVDLAVDYGYNTQRPYQGALPDEFPAF
                     WQRVIPINRTAKTAEVARGVV"
     gene            793..>1541
                     /locus_tag="VHT22_09215"
     CDS             793..>1541
                     /locus_tag="VHT22_09215"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX3137777.1"
                     /translation="MKMLLIVSAFAALTWPGAPPFAQSYPVKPVRYVVAFAAGDSPDI
                     VARLVADRMSRLWDQQMVVENRVGAGGTIAGAAVANAAPDGYTLFHCNIASSAIASAI
                     YPKLPYDPVRDFAMVSRIATTANALIVHPSLPAKSVAEFIAYAKAHPGKLSYGSPGVG
                     TSPHLSMELFKTTSGINVVHIAYKGAMPALADLMGGQIPVMLANLPALLPHIVSGKIR
                     ALAVTSATRSARLPAVPTVLESGVADYVVTS"
BASE COUNT          264 a          491 c          495 g          291 t
ORIGIN      
        1 tgacgacgcc gcgcgcgact tcggcggttt tcgccgtacg attgattgga atcacgcgct
       61 gccagaacgc cggaaattca tccggcagcg cgccttgata cgggcgttgc gtgttgtagc
      121 cgtagtcgac ggcgaggtct actgccagca cgcgcacttc cagtacgtcg ccgggcagcg
      181 cgccatttac cgcaatgggc ccggtaagta tgtgtccgcc ggggccgcga tcttttacgt
      241 ccctcgcgat ggcgcgcacg tactcgggca ccgcactggg cggcacgacg ccggatttgt
      301 cgactgcatc gggcgcgatt gcgccggcac tttcaagcac gacgatgtcg cccgattcga
      361 cggttagcac cggcttgagc gccgcgttga aatttccgac atggaccgtt tccgggctcg
      421 gctcagctcg tgcacgcgct ggctcagtgc ctcggtgctg agcagcagcg caaacgaaaa
      481 gaaaaatgcg agcgtggcgt aacggtcagg caggcgatgc gcccgcggca tcgcggtcat
      541 cgcggctgcc gcggcgcccg ccgccagcaa tgtgcgtcga ttggtatcca tatcgtcctc
      601 cctcgccgat gaattttaca gtcgtggcgc cgttcgccga acgaatcttg tgcgcgcgtg
      661 aaagcttgtc cctgcaggcc ggcgcagtca agttcgcggg cgccggcggc aatttaggca
      721 cggatatgcg aacacgcttc catagtacgg cgtcggtgtg tcaggatagg cgcaaaactg
      781 gggagggcga tcatgaaaat gttgctcatt gtcagcgcgt ttgccgccct gacatggccg
      841 ggcgcgccgc ctttcgcgca atcctatccg gtcaagccgg tgcgctacgt ggtggccttc
      901 gcggccggcg attcgcccga cattgtcgcg cggctagtcg cggaccgcat gagccggctg
      961 tgggaccagc agatggtggt ggaaaaccgc gtgggtgcgg gcggcacgat tgccggggcc
     1021 gccgtggcca acgcggcgcc cgacggctac acgctgttcc attgcaatat cgcatccagc
     1081 gcaatcgcgt cggcgatata cccgaaactg ccgtacgatc cggttcgcga tttcgcgatg
     1141 gtgtcgcgca tcgccaccac cgccaacgcg ctgatcgtgc atccatcgct gccggcgaaa
     1201 tcggtcgcgg aattcatcgc gtacgcgaag gcccatcccg gcaaattaag ttacggctcg
     1261 ccgggcgtcg gcacttcccc gcatttgtcg atggaactct ttaaaacgac cagcgggatc
     1321 aacgtggtgc acatcgccta taaaggcgcg atgccggcgc tcgccgacct gatgggcggg
     1381 cagattccgg tgatgctagc caatctgccc gcgctgctac cgcacatcgt ttccggcaag
     1441 atacgcgcgc tcgccgtgac ctccgcgacg cgctcggcac ggctgccggc ggtcccgacc
     1501 gtgctcgaat caggggtcgc agattacgtc gtaacttcgt g
//
LOCUS       DATANS010000627         1858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_30812, whole genome shotgun sequence.
ACCESSION   DATANS010000627 DATANS010000000
VERSION     DATANS010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30812"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VHT22_09220"
     CDS             complement(<1..555)
                     /locus_tag="VHT22_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEX3137778.1"
                     /translation="MTSSVSTSENRFALAAGALADVAAQVLDAARAGGATAAETEVSQ
                     AFGQSVTVRKGDVETIAYNRDKGIGVTVYVGQRRGHASTADFEPGAIRDTVTKALTIA
                     RYTADDSCAGLADPTLLAHDWTDPDLSHPWPLTVEQAIELGRECEAAALGVDARINNS
                     EGASVSLHEAEFVYANSNGFSGGYA"
     gene            631..1251
                     /gene="yjgA"
                     /locus_tag="VHT22_09225"
     CDS             631..1251
                     /gene="yjgA"
                     /locus_tag="VHT22_09225"
                     /inference="COORDINATES: protein motif:HMM:NF003593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HEX3137779.1"
                     /translation="MRRSDETPRRTGQEGRGPSSPGSRHAAPAIGAPDRSTPDLPSDE
                     VAILSKTRRKAEMHDLQSLGETLVQLSAARLSELGLPERLADAIEQARGIRKHEARRR
                     QMQYIGRLMRDVDPEPIRTRLAQWGAAPAAEKARLAAVERWRERLLVDSAALDQLCAA
                     APHADRALLSALVTRVQGERARAAPPHAYRELFRVLNTLLSTVHAT"
     gene            1289..1849
                     /gene="mog"
                     /locus_tag="VHT22_09230"
     CDS             1289..1849
                     /gene="mog"
                     /locus_tag="VHT22_09230"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HEX3137780.1"
                     /translation="MTEVASLLIGLVSVSDRASAGVYEDKGIPGLTDWFGAALTTPWR
                     METRLIPDEQALIERTLIDLVDTVRCHLVLTTGGTGPAARDVTPEATLAVAHKVLPGF
                     GEQMRQVSLRYVPTAILSRQVGVIRGGALILNLPGQPKAIKETLDGVFPAVPYCIDLI
                     GGPYIETKDEVCKVFRPKSALRPPSV"
BASE COUNT          300 a          633 c          627 g          298 t
ORIGIN      
        1 cgcgtagccg cccgaaaatc cgttgctgtt cgcgtagacg aattccgcct cgtgcagcga
       61 taccgacgcg ccttcggaat tgttgatgcg cgcatcgacg ccgagcgccg cggcctcgca
      121 ttcgcgtccg agctcgatcg cctgctcgac ggtcaacggc caggggtgac tcaggtccgg
      181 atcggtccag tcatgcgcga gcagcgtcgg gtcggccagc cctgcgcagc tgtcgtcggc
      241 ggtgtagcgt gcgatggtca gcgccttggt gacggtgtcg cgtatcgcgc ccggctcgaa
      301 atcggctgtg ctggcgtggc ctcgtcgctg gccgacgtag acggtgacgc cgatcccctt
      361 gtcgcggttg taggcgatgg tctcgacatc ccccttgcgc acggtaacgc tctggccgaa
      421 agcctgcgag acttcggtct cggcggcggt cgcgccgccc gcacgcgcgg cgtccagcac
      481 ttgtgcggcg acatcggcga gcgcgccggc ggcgagtgca aaacgattct cggacgtgct
      541 cactgagctg gtcatcgacg gactcgaagc ggaaggcgga cccctgatcg gttatgatag
      601 caaacccctt tcgcgacccc tggcgccgcc atgcgacgat ccgacgaaac accccggcga
      661 acgggacagg agggacgcgg accgtcctct cccggcagcc gccacgcggc gccggcgatc
      721 ggcgcccccg accgatcgac gccggatctg cccagcgacg aggtcgcgat cctgtcgaaa
      781 acgcgccgca aggccgagat gcacgatctt cagagcctag gcgagacgct ggttcaactt
      841 tccgccgcgc ggctgtccga gctgggcttg cccgagcgcc tcgccgacgc gatcgagcag
      901 gcgcgcggga tccgcaagca cgaggcacgg cgcaggcaaa tgcaatacat cggcaggctg
      961 atgcgcgatg tcgatcccga gccgatccgg actcggctcg cgcagtgggg cgccgcgccg
     1021 gccgccgaaa aagctcgtct ggccgcagtc gagcgctggc gcgagcgcct gctcgtggac
     1081 tcggccgcgc tcgatcaact gtgcgccgct gctccgcatg ccgaccgcgc actcctttcg
     1141 gcgctggtga cgcgcgtgca aggcgagcga gcgcgggccg caccgccgca tgcttatcgt
     1201 gagctttttc gcgttctgaa cacgttgttg tcgacggtgc acgctacgtg atgccgggcg
     1261 cagagcgcga tgtcgctgga acttgccgat gaccgaggtc gcatcgcttt tgataggcct
     1321 ggtctccgtc agcgaccgcg catccgccgg cgtatacgag gacaagggca ttccgggcct
     1381 caccgactgg ttcggtgcgg cgctgacgac gccgtggcgg atggaaacgc ggctgatccc
     1441 cgatgagcaa gcgctgatcg agcgcacgct gatcgatctc gtcgacaccg tgcgctgcca
     1501 tctggtgctg acgacgggag gcaccgggcc cgcggcgcgc gacgtcacgc cggaagcgac
     1561 gctggccgtc gcgcacaagg tgctcccggg cttcggcgag cagatgcgac aggtaagcct
     1621 acgatacgtg ccgacggcga tactgtcgcg ccaagtcggc gtcatccgcg gcggcgctct
     1681 gatcctcaac cttcccggac agccgaaggc catcaaggaa acgctcgacg gcgtctttcc
     1741 cgccgtgccc tactgcatcg acctgatcgg cggcccttac atcgagacga aggacgaagt
     1801 ctgcaaagtc ttccgtccga agagcgcact gcgcccgccg tcggtgtgaa accgcgcg
//
LOCUS       DATANS010000628         3946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_30828, whole genome shotgun sequence.
ACCESSION   DATANS010000628 DATANS010000000
VERSION     DATANS010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30828"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VHT22_09235"
     CDS             complement(<1..1023)
                     /locus_tag="VHT22_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX3137781.1"
                     /translation="MPAPAKSSPKTKTAAPASAPPTASRAVKKGDHVFLVDGSGYIFR
                     AYHAIRFEPRTADGTHVNAVYGFCSMLWKLLMEMKADERPTHLAVIFDKSEKTFRTEM
                     YKEYKAHRPPPPDDLIPQFPLIRDAVNAFEIPCLEQAGYEADDLIATYARIACEAGAT
                     ATIVSSDKDLMQLVNECVVMYDTMKDVRIGVPEVVEKFGVPPAKVIEVQALIGDSTDN
                     VPGVPGIGVKTAAQLIGEYGDLETLLKRATEIKQDKRRQTLIDNAEAARMSKKLVTLD
                     DRVPLDVPIENLGVREPDYKELIAFLKAMGFKTLTQRVADKAGVDASQVEAAQATSPA
                     PKSSGVQ"
     gene            1189..2499
                     /locus_tag="VHT22_09240"
     CDS             1189..2499
                     /locus_tag="VHT22_09240"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX3137782.1"
                     /translation="MRVAMIGTGYVGLVSGACFADFGHEVVCVDKDKAKIAALTRGEV
                     PIYEPGLNDLVASNARAGRLKFTTELPGAVAAADAVFIAVGTPSRRGDGFADLSFVHE
                     AAREIARALDGFTVVITKSTVPVGTGDEVERIIRETRSDADCAVVSNPEFLREGAAIQ
                     DFKHPDRIVIGTPDTRAREVMSELYRPLYLNRSPILFTDRRTAELIKYAANAFLATKI
                     TFINEIADLAEKVGADVQEIARGIGLDNRIGSKFLHAGPGYGGSCFPKDTVALIKTAQ
                     DHAAPLRIVEAVAAVNDARKRAMARKVSAALGGELRGKTIAILGLTFKPNTDDMREAP
                     SIPLITALQDLGAKVRVYDPIGMEQAKGLIGNITFCNDSYSCAEGASALVIVTEWEQF
                     RALDFGRLKGLMAQPVLVDLRNVYRPDEIARHGFVYESIGRPRK"
     gene            2631..3851
                     /gene="hrpB"
                     /locus_tag="VHT22_09245"
                     /pseudo
     CDS             2631..3851
                     /gene="hrpB"
                     /locus_tag="VHT22_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611465.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
BASE COUNT          666 a         1248 c         1268 g          764 t
ORIGIN      
        1 ctggactccg gatgacttcg gtgcaggcga ggttgcttgc gcggcttcga cctggctcgc
       61 gtcgacgccg gccttgtcgg cgacgcgctg cgtcagcgtc ttgaaaccca tcgccttgag
      121 gaatgcgatc agctccttgt aatcaggctc tctcaccccg aggttttcga tcggcacatc
      181 gagaggcacg cgatcatcga gggtgacaag ttttttcgac atgcgcgccg cctcggcatt
      241 gtcgatgaga gtctggcggc gcttgtcctg cttgatctcg gttgcccgct tgagcagcgt
      301 ttcaaggtcg ccgtactcgc cgatcagctg ggcggcggtt tttacgccga tgcccggtac
      361 gccgggcacg ttgtcggttg aatcgccgat caacgcctga acctcgatca ccttcgccgg
      421 cggtacaccg aatttttcga cgacttccgg aacgccgatg cgcacatcct tcatcgtgtc
      481 gtacatgacc acgcactcgt tcacgagctg catgaggtcc ttgtcggatg agacgatggt
      541 ggcggtcgcg ccggcttcgc aggcgatgcg cgcatacgtt gcgatcagat cgtcagcctc
      601 gtacccggcc tgctcgaggc aggggatctc gaaggcattc accgcatcgc ggatcagcgg
      661 aaactgtgga atgaggtcgt cgggaggagg cggtcggtgc gccttgtact ccttgtacat
      721 ctccgtgcgg aacgtctttt ccgatttgtc gaagatcacc gcgagatggg tcggccgctc
      781 atccgccttc atctccatca gcagcttcca cagcatgctg cagaaaccgt agaccgcgtt
      841 gacatgcgtg ccatcggcgg tgcgcggctc gaacctgatc gcatggtaag cgcggaagat
      901 gtacccggaa ccgtccacca ggaatacgtg atcgcccttt tttacggcac gggacgccgt
      961 tggcggggct gaggctggtg cggccgtttt tgttttgggg ctcgattttg cgggggctgg
     1021 catgtgcgga atgtgggccc gcaggccgcg ttaatcaatc ggccagaatc tccggtcaca
     1081 gaaaatgcac agaagctgtc gcctctcgcc actcgcgatc gcgtctgcta tgcaggccgc
     1141 ggtccggctt tgaccgatgc ggtttgccac ctccatgatg gatcttaaat gcgcgttgcc
     1201 atgatcggca ccggctatgt cggcctcgtt tccggtgcgt gctttgcaga cttcggccat
     1261 gaggtcgtct gcgttgacaa ggacaaggca aagatcgcgg cgctgacccg cggcgaagtg
     1321 ccgatctatg agcccggcct caatgacctg gtcgccagca atgcgcgcgc cggcaggctg
     1381 aaattcacga cggaactgcc tggcgcggtt gccgcagccg atgcggtctt cattgcggtt
     1441 ggtacgccct cacgccgcgg cgacggtttc gccgacctga gcttcgttca cgaggccgca
     1501 cgggagatcg cgcgcgcgct cgacggcttc acggtcgtga tcaccaaatc gaccgtgccg
     1561 gtcggtaccg gcgacgaggt ggaacgcatt atccgcgaga cacggtccga tgccgactgc
     1621 gcggtcgtct cgaatccgga attcctccgc gaaggcgcgg ccattcagga tttcaagcat
     1681 cccgaccgta tcgtcatcgg cacgcccgac acgcgcgcgc gtgaggtcat gagcgagttg
     1741 tacaggccgc tctatctcaa ccgttcgccg atactcttca ccgaccggcg cacggccgag
     1801 ctcatcaagt acgcggccaa cgctttcctc gccaccaaga tcaccttcat caacgagatt
     1861 gccgatctcg ccgagaaagt gggtgccgac gttcaggaaa tcgcgcgcgg gatcgggtta
     1921 gacaaccgga tcgggtcgaa gtttctgcac gccggccccg gctacggcgg ctcgtgcttt
     1981 cccaaggaca cggtcgcgct gatcaagacg gcgcaggacc acgccgctcc gctgcgcatc
     2041 gtcgaggccg ttgcagccgt caacgacgcg cgcaagcgcg cgatggcgcg caaggtttcg
     2101 gctgcgctcg gcggcgaact gcgcggaaaa acgatcgcca tcctcggcct cacattcaaa
     2161 ccgaacaccg acgacatgcg cgaggcgccg tcgatcccgc tgatcacggc gttgcaggac
     2221 ctgggcgcga aggtgcgcgt ctacgatccg atcggcatgg agcaggccaa aggcctcatc
     2281 ggcaacatca cgttctgcaa tgactcctat tcctgcgccg agggcgcttc ggcgctggtg
     2341 atcgtcaccg agtgggaaca attccgcgcg ctggatttcg gccggctgaa ggggctgatg
     2401 gcgcagcccg tgctggttga tctgcgcaac gtctaccggc cggatgaaat tgcccgccac
     2461 ggctttgtct atgagagcat cggacggccg cgcaaatgat gtctgccagc gtcgtctttg
     2521 ctagaagttg tcgtcccccg cgcgagcggg ggacccggta atcatccaca acgcgtttct
     2581 tgcttccgcg gttaccgatt catccggcac tccggatgat gacgacgaag atcatgcgaa
     2641 ctttcgatac atctctgcca atcgacgtcg cgctgccgca gctcaccgcg gcgctcgcgc
     2701 gcaacgatgc cgccgtgctg gtcgctccac caggcgccgg caagacgacg cgcgtgccgc
     2761 tggtgctgct cgacgagccg tgggcgaaag acaggaaaat tctggtgctg gagccgcgcc
     2821 ggcttgcggc ccgcgctgcc gccgcgcgta tggcgtcgac cttgcgcgaa caggtcggcg
     2881 acaccgtcgg tcttcgcgtg cggttcgatt cgcaggtgac aaaacgcacg cgcatcgaga
     2941 tcgtcactga aggcgtgttc acgcggcttg ttctcgacga tccttccctc gatggcattg
     3001 ccgccgtgct gttcgacgaa ttccacgagc ggtcgctcga tgccgatctt ggattggcgc
     3061 tggcgcgcga cgcgcagctc ggcctgcgcc cgaatctcaa gctgctggtg atgtcggcaa
     3121 cgctcgatgg cgcgcgggtt gcggcgctgc tcggcaatgc gccggtggtc gaaagcgaag
     3181 gacgcgcctt tgcggtggag acacgttatc tggggcgcga tcaacgcgcg ccgattgaac
     3241 gtcaggtggc cgatgcggtc gagcgcgcgc tgcgggccga aagcggttcg gccctggtgt
     3301 ttctgccggg tgccggcgag atccgccgca ccgaggagct tttgaaagag cgcgtgcgcg
     3361 acgcggccat cgacatcgtc ggtcttttcg gtgcgcttga tgcgcgcgag caggatcgcg
     3421 cgatttcgcc atcgccgccg gggcggcgca aggtcgtgct ggcaacctcg attgccgaaa
     3481 cctcgatcac catcgaaggc gtgcgcgtcg tcgtcgacag cgggctcgcg cgggtgccgc
     3541 gctacgagcc agacgtcggc ctgacacgac tggaaaccgt gcgcgtctcg cgcgcggccg
     3601 ccgatcagcg gcgtggccgc gccggccgta ccgagccggg catctgttac cggctctggg
     3661 acgagccgca gaccggatcg cttgaggcct atacgcggcc ggaaatattg tccgccgatc
     3721 tgtcgtcgtt cgcgctcgat ctcgcccaat gggggacgac cgatacaagc cagctcgcat
     3781 tcctcgatgc gccaccgcag gccgcgatga atgaggcctg gaccctgctc ggcgagatcg
     3841 tcgacgccga tttgttgcag tgatgttttt cccgtgagga acggcgccag ccattcggag
     3901 gccgtgcgcg acaatgcgtc atccgacaga tcaggccatt catcgc
//
LOCUS       DATANS010000629         4341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_452351, whole genome shotgun sequence.
ACCESSION   DATANS010000629 DATANS010000000
VERSION     DATANS010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452351"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..903
                     /locus_tag="VHT22_09250"
     CDS             61..903
                     /locus_tag="VHT22_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX3137783.1"
                     /translation="MQLNAQQVETFDRDGYLFFDSLFTSREIGVLREEVPRLYAQRRI
                     ENVREKGSDAVRTNFAAHLYSAPFARLARHPRMIEPVVALFGESVYMHQFKINGKMAF
                     DGDVWQWHQDFGTWKNDDDMPEPRAMNIAIFLDEVNEFNGPLMFIPGSHKLGVLDADH
                     DTSTTSYPLWTINHATIASLVERGGIVAPKGPAGSMLLFHSCLVHASTSNLSPWNRVS
                     VYLSLCAVSNHIRRFKRPEYIAHRDFTPIECLPDDCLLRDYPVALPWRDGTPEAALQP
                     ALAG"
     gene            918..2282
                     /locus_tag="VHT22_09255"
     CDS             918..2282
                     /locus_tag="VHT22_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HEX3137784.1"
                     /translation="MSLHAKLNERAAQGRPLSVGMIGAGKFGAMYLAQVPKTPGVHVV
                     AIADLDPAAALSNLARVGWPRERSAAPSIDAALAQGATHVGDDWQALVRHPKIDIVVE
                     CTGNPIAAVEHCLEAFVQRKHVVNVTVEADALCGPLLARKAAEAGVVYSLAFGDQPAL
                     ICDLVDWARTAGFGVVAAGRGHKWLPHFAQSTPETVWGHYGLTPEQARIGGLNAKMFN
                     SFLDGSKPAIECTAICNATGLSTPPDGLTYPPASIDQIPNVCRPIDEGGVLHAKGQVE
                     VISSVERDGRAIAYDIRFGVFVVFEGDTEYLRNCFREYMVRTDDSGRYACLYKRWHLI
                     GLEVGISVASVGLRGEATGAATCFNADAVATAKRDLKAGETLDGEGGYTVWGKLLPAA
                     ASLDGGALPLGLAHGVRLLRPVRQGEVLRWSDVMIDETLTACRARREMEDLFGRASPR
                     ERAA"
     gene            complement(2340..4091)
                     /locus_tag="VHT22_09260"
     CDS             complement(2340..4091)
                     /locus_tag="VHT22_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013555276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3137785.1"
                     /translation="MITGDMLRAVPLLAGVADSELNTIARRAADISLQTNEWLIQEGE
                     LPAFFITLSGRLTVYKSVAGVERAITEYRPGDYGGEVPLLLGSPAIASIRAAEPSRVC
                     RLDAADFRELIVACPELNARLLQTMATRIEGLQQLATEAPLATVTIIGHRYDLACHAL
                     RDFLARNHVAFRWYDPRDPEARAGLAATPQPDEIYPVVIFPDGARLVTPSFRDVAEHL
                     GLQTAPSRREYDVAIIGAGPAGLAAAVYGASEGLCTILIERQAPGGQAGTSSRIENYL
                     GFPTGVAGDELAARALRQAKRFGAEILVARGATGIDTATRDAPHAILLEDGERVQTRS
                     IVIATGVSWRELDLPNAPTLVGRGLYYGAARTEAPNTRGLDIFMIGGGNSAGQAAMFF
                     ANYARTVTLIVRGPSLERSMSQYLIDQLATRPNIRVESRSQVVALEGTDRLEAIVIEN
                     RATGRRRREVTEALFVFIGADAETTWLPQAVIRDGRDFLCTGRDVMDLVAGQQGSWPL
                     SRDPYLLETSVPGVFAVGDVRHGSVKRVASGVGEGSMAIAFVHQYLADPPEFADPAVD
                     TPTRAADAAVLPQALGV"
     gene            complement(4138..>4341)
                     /locus_tag="VHT22_09265"
     CDS             complement(4138..>4341)
                     /locus_tag="VHT22_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX3137786.1"
                     /translation="KTGDDWVHLRLCLTCGHVGCCDESRNKHATRHFKETGHPIIQSF
                     EPGEAWGWCFVDRALFDQLPVTY"
BASE COUNT          728 a         1448 c         1451 g          714 t
ORIGIN      
        1 gcattgtgtc attcatggaa gccggggccg gcgcggccgg gtcggcgaag gagtcagagc
       61 atgcaattga acgcgcaaca ggtcgaaacc ttcgatcgcg acggctacct ctttttcgac
      121 tcgttgttca cgtcacgcga aatcggcgtg ctgcgcgaag aagtgccgcg actctatgcg
      181 cagcggcgga tcgagaacgt ccgggaaaag ggcagcgatg cggtgcgcac caacttcgcc
      241 gcgcacctgt acagcgcgcc gttcgcccga ctggctcgac atccacgcat gatcgagccg
      301 gtcgtcgcgc tattcggcga gtcggtctac atgcatcagt tcaagataaa cgggaagatg
      361 gctttcgacg gcgacgtctg gcagtggcac caggatttcg gcacctggaa gaacgacgac
      421 gacatgcccg agccgcgggc gatgaacatc gcgatatttc tcgacgaggt caacgagttc
      481 aacggcccgc tgatgttcat cccgggcagc cacaagctcg gcgtcctgga cgccgaccac
      541 gacacgtcga cgaccagcta tccgctgtgg acgatcaatc atgcgacgat cgccagcctg
      601 gtcgagcgcg gcggcatcgt cgcgccaaag gggcccgcgg gatcgatgct gcttttccac
      661 tcctgcctgg tgcatgcctc gacgtcgaat ctgtcgccgt ggaatcgcgt cagtgtgtat
      721 ctgagcctgt gcgcggtgtc gaaccacatc cggcgtttca agcgtcccga atacatcgcg
      781 caccgcgatt tcacaccgat cgaatgtctg cccgacgatt gcctgcttcg cgattacccg
      841 gtcgcgctgc cgtggcgcga cggcacgcct gaagccgctc tgcagccggc actggccggc
      901 tgaatgcgga cgcgcgcttg agccttcacg ccaagctgaa cgagcgcgct gcgcagggca
      961 ggccgctgtc cgtcgggatg atcggcgccg gcaagttcgg cgcgatgtac ctggcccagg
     1021 tgccgaagac gccgggcgtg cacgtggtcg ccatcgccga tctcgatccg gccgcggcgc
     1081 tgtccaacct cgctcgcgtc ggatggccgc gcgagcgcag cgccgcgccg tcgatcgatg
     1141 ccgccctcgc gcaaggcgcg acgcatgtcg gcgacgattg gcaagcgctg gtacggcatc
     1201 cgaagatcga catcgtcgtc gaatgcacag gcaatccgat cgcggcggtc gagcactgtc
     1261 tcgaagcttt cgtccagcgc aagcatgtgg tgaatgtgac cgtcgaagcc gatgctctct
     1321 gcgggcccct gctcgcccgc aaggcggccg aggccggcgt cgtctacagc ctcgccttcg
     1381 gcgatcagcc ggcattgatc tgcgatctcg tcgactgggc gcggaccgct ggcttcggcg
     1441 tcgttgcggc aggccgcggt cacaaatggc tgccgcactt cgcgcagtcg acgccggaga
     1501 ccgtctgggg ccactatgga ctcacgcccg agcaggcgcg gatcggcggc ctcaacgcca
     1561 agatgttcaa ctcgtttctc gatggctcga agccggcgat cgagtgcacg gcgatatgca
     1621 acgcgacggg gctgtcgacg cctccggacg gcttgacgta tccgccggca tcgatcgatc
     1681 agatcccgaa cgtgtgtcga ccgatcgatg aaggcggcgt gctgcacgcc aagggccagg
     1741 tcgaagtgat ctcgtcggtc gagcgcgacg gccgcgcgat cgcgtacgac atccgcttcg
     1801 gcgtcttcgt cgtgttcgaa ggcgataccg aatacctgcg caactgcttt cgcgagtaca
     1861 tggtccgaac cgatgacagc ggccgctacg cctgccttta caagcgctgg catctgatcg
     1921 gcctcgaggt gggcatctcg gtggcgtcgg tcggcttgcg cggcgaggct acgggtgctg
     1981 cgacctgctt caacgccgac gccgtggcca ccgccaagcg agacctcaag gccggcgaga
     2041 cgctcgacgg cgaaggcggc tacacggtct ggggcaagct gctgccggcg gccgcgtcgc
     2101 tggacggcgg cgcgctgccg ctcggcctcg cacacggcgt gcgcctgttg cgcccggtca
     2161 ggcaggggga agtgcttcgc tggtccgatg tgatgatcga cgaaacgctg accgcctgtc
     2221 gtgcccgccg cgaaatggaa gacctgttcg gccgcgcgtc gccgcgcgag cgcgctgcgt
     2281 agctcgtatc gccccgggcg cggttgttcc tgttgcgcgc gaccgctaaa gttcgcccgc
     2341 tagaccccca gcgcttgcgg aagcacggcg gcgtccgccg cgcgagtcgg cgtgtcgacg
     2401 gccgggtctg caaactcggg tggatcggcc agatattgat gcacgaatgc gatcgccatg
     2461 ctgccctcgc cgacgccgga agccacgcgc ttgaccgagc cgtggcgcac gtcgccaacg
     2521 gcgaacaccc cgggcacgct ggtctcgagc aggtacggat cgcgcgacag cggccacgaa
     2581 ccctgctggc ccgcgaccag gtccatcaca tcgcggccgg tgcaaagaaa gtcgcggccg
     2641 tcgcgaatga ccgcctgcgg gagccatgtc gtctctgcgt cggcgccgat aaacacgaac
     2701 agcgcctcgg tgacctcgcg tcggcggcgg ccggtcgcgc gattctcgat gacgatcgcc
     2761 tccaatcgat cggttccctc gagcgcgacc acttggctgc gcgattcgac gcgaatgttg
     2821 gggcgggtcg caagctggtc gatcaggtat tgcgacatgc tgcgctcgag cgacggaccg
     2881 cgcacgatca gcgtgaccgt gcgcgcgtaa ttggcgaaaa acatcgccgc ctgccccgcg
     2941 gagttgccgc cgccgatcat gaagatgtcg aggccgcgcg tattgggcgc ctcggtgcgt
     3001 gccgcaccgt aataaaggcc gcgtccgacc agcgtcggcg cgttcggcag atcgagctcc
     3061 cgccaggaga cgccggtggc gatgacgatg cttcgcgtct gcacgcgctc gccgtcctcg
     3121 agcaggatcg cgtgtggcgc atcgcgcgtc gccgtgtcga tgccagtcgc gccgcgcgcg
     3181 accagaatct cggcgccgaa gcgcttcgcc tggcgcagcg cccgcgcggc gagctcgtcg
     3241 ccggcaacgc cggtcgggaa accaagatag ttttcgatgc gcgacgacgt gcctgcctgg
     3301 ccgcccggcg cctggcgctc gatcaggatc gtgcacagac cttcggaagc gccgtacacg
     3361 gcggcggcca gacccgccgg cccggcgccg atgatcgcga catcgtattc gcgccgcgat
     3421 ggggccgtct gcaatcccag gtgctcggcg acgtcgcgaa atgacggcgt gaccaaccgc
     3481 gcgccgtcgg gaaagatcac caccgggtag atctcatccg gctgcggcgt cgccgccaat
     3541 ccggcccggg cctcgggatc gcgcgggtcg taccagcgga acgcgacatg attgcgcgcc
     3601 aggaaatcgc gtagcgcgtg acaggctaga tcgtagcggt ggccgatgat ggtcaccgtc
     3661 gccagcggag cctcggtcgc gagctgctgc agcccctcga ttcgcgtcgc catcgtctgc
     3721 agcaggcgcg cgttgagctc cgggcaggca acgatcagct cgcggaaatc cgctgcgtcg
     3781 agccggcaga cccgcgaagg ctcggcggcc cggatgctcg cgatcgccgg cgagcccagc
     3841 agcagcggca cctcaccgcc gtagtccccg gggcggtact cggtgatcgc gcgctcgacg
     3901 ccggcgaccg acttgtagac ggtcagtcga ccggagaggg tgataaagaa cgccggcagc
     3961 tcaccttcct ggatcagcca ttcgttggtc tgcagcgaga tgtccgcagc tcgccttgcg
     4021 atggtgttga gctcgctgtc ggcaacgcct gccagcagcg gcacggcgcg caacatgtca
     4081 ccggtaatca tcggcgcatc atcgcatgat ttgcgacgct gctcgcgatc gagaaagtca
     4141 gtacgtcacc ggcagctggt cgaacagagc acggtcgacg aaacaccatc cccacgcttc
     4201 gccgggctcg aacgactgga tgatcggatg gccggtctcc ttgaaatgac gcgtcgcatg
     4261 cttgtttctc gactcgtcgc agcatccgac gtggccgcag gtcaggcata gacgcaggtg
     4321 cacccagtcg tcgccggtct t
//
LOCUS       DATANS010000630         1762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_410436, whole genome shotgun sequence.
ACCESSION   DATANS010000630 DATANS010000000
VERSION     DATANS010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410436"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VHT22_09270"
     CDS             <1..787
                     /locus_tag="VHT22_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX3137787.1"
                     /translation="CRGGAALVDAAGGLAFPACALAVAEAIRRAREFGVSFVGVTNSH
                     HFGVAACHLAPVAAAAMVGLALGNSPAAMAVAGGRHPVLGTNPVAAIFPRKNGAPLTI
                     DLSLSQAARGKVMIAAKEGRPIPLGWALDADGKPTTDARAALAGSMLAVGGDKGATLA
                     LVVELLACALTGAAMGFEADSFLIDEGNRPGVGQAFLVIDPAALAGMPVYLERVESLI
                     AEMEKDPGVRLPGYRRNALSAAAARDGIEIDPALHRRLLELAG"
     gene            complement(822..>1762)
                     /locus_tag="VHT22_09275"
     CDS             complement(822..>1762)
                     /locus_tag="VHT22_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX3137788.1"
                     /translation="PDLDGNFTFSRFNPAVGINFNPSPSLTLYSSYSQGMRAPSPVEL
                     TCSDAAAPCKLPNIFIADPPLKKVVSRTVEAGARGKLDMTTQWAIAAYRTDLDDDIQF
                     ISSGAGAVNAGFFQNVGKTRRQGVELWGGTRVGALSLNLRYSHTDATFRSAFVAASPN
                     NSAADDNGAIAVTPGNRIPGIPSDSAKLRVDYDFDDRFSVGGSVVYASSQYARGDENN
                     QDRHGTVPAYTVVDLDARYQAAKDIEIFVRIANAFDRRYQNIGVLGSNVFTGPNRTFG
                     PSSGNDAVAEQFRGIGAPRGIWIGLRYSFGAPARSN"
BASE COUNT          290 a          556 c          605 g          311 t
ORIGIN      
        1 ttgccgtggc ggcgccgcgc tggtcgacgc ggccggcggc ctcgcgtttc cggcctgcgc
       61 gctcgccgtc gccgaagcga tccggcgggc gcgcgaattc ggcgtgagct tcgtcggcgt
      121 caccaacagc catcatttcg gcgtcgccgc ctgtcatctg gcgccggtcg cggcggcggc
      181 aatggtcggc ctggcactgg gcaactcgcc ggcggcgatg gccgtcgcgg gcggccgaca
      241 cccggtgctc gggaccaatc cggtcgccgc catctttccg cgcaagaacg gtgcgccgct
      301 gacgatcgac ctctcgcttt cgcaggccgc gcgcggcaag gtgatgatcg cggcgaagga
      361 aggccgaccg attcctttgg gttgggcgct cgacgccgac ggcaaaccga cgaccgatgc
      421 acgggcggcg ctggccggat cgatgctggc ggttggcgga gataaaggcg cgacgctcgc
      481 cttggtcgtc gaattgctcg cctgcgcgct gaccggcgcc gccatgggat tcgaagccga
      541 ttcgttcctg atcgacgaag gcaatcgccc cggcgtcgga caggccttcc tggtcatcga
      601 tccggcggcg ctcgcgggca tgccggttta tctcgagcgg gtcgaatcgc tgatcgcgga
      661 aatggaaaag gacccgggcg ttcggttgcc cggctatcgg cgcaatgcgc tgtcggctgc
      721 ggccgcacgc gatggcatcg agatcgatcc ggcgctgcac aggcgcttgc tcgagctcgc
      781 cggctgatgc atcgtccggc gaccggcctg cgcgcgggtc tttagttgct ccgcgcgggc
      841 gctccgaacg aatagcggag tccgatccag atgccgcgcg gcgcgccgat tccgcgaaac
      901 tgctcggcca ccgcgtcgtt tccggacgag ggcccgaagg ttcgattggg cccggtgaag
      961 acattggaac ccagcacacc gatgttctgg tagcggcggt caaacgcgtt ggcgattcgg
     1021 acgaagatct cgatgtcttt ggcagcctgg tagcgcgcat cgagatcgac taccgtgtac
     1081 gcaggcaccg tgccatgccg gtcctgattg ttctcgtcgc cgcgagcgta ctggctggag
     1141 gcatagacga cgctcccgcc gacggaaaaa cggtcgtcga agtcgtaatc aacgcgtagc
     1201 ttcgctgaat ccgacggaat accgggaatg cgatttcccg gagtgacggc gattgcgcca
     1261 ttgtcgtcgg ctgccgagtt gttcgggctc gcggcgacga aggccgagcg gaacgtcgca
     1321 tcggtatggc tgtatcgcag gttgagcgac aacgcgccga cgcgcgtccc gccccagagc
     1381 tctacacctt gccggcgggt cttgccgaca ttctggaaaa aacccgcatt tacggcgccg
     1441 gcaccgctgg agatgaactg gatgtcatcg tcgagatcgg tacgataagc ggcaattgcc
     1501 cattgcgtgg tcatgtccag cttgccgcgc gcacccgcct cgacggtcct ggagacaacc
     1561 ttcttcaacg gcgggtcggc gatgaatatg ttcggcagct tgcatggcgc agcggcatcg
     1621 gagcaggtga gctccaccgg agacggcgcc cgcatgccct ggctgtagct gctatacagg
     1681 gtcagcgacg gagagggatt gaaattgatc cccaccgccg gattgaagcg ggaaaacgtg
     1741 aaattgccgt ccaggtccgg ac
//
LOCUS       DATANS010000631         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_577432, whole genome shotgun sequence.
ACCESSION   DATANS010000631 DATANS010000000
VERSION     DATANS010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577432"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VHT22_09280"
     CDS             complement(<1..756)
                     /locus_tag="VHT22_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX3137789.1"
                     /translation="MPQSFPWFTVIFLLALGVSLAMQLWLARRQVQHVAANRAAPPAH
                     FATRITLAAHQKAADYTIARTRLGMLETVLEAIILLALTLGGGLAALALRADLLPVGT
                     LWQDVALVVAVTLLAALVGLPISWYRTFVIEQRFGFNRMTLRLWLADLAKGALLGALL
                     GVPLLTLVLWLMARAGALWWLYAWLVWVAFQLLMLVLFPTVIAPLFNKFQPLADEPLR
                     ARVERLLARCGFAAKGLFVMDGSRRSSHGNAYFT"
     gene            821..1390
                     /gene="orn"
                     /locus_tag="VHT22_09285"
     CDS             821..1390
                     /gene="orn"
                     /locus_tag="VHT22_09285"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEX3137790.1"
                     /translation="MAQDANLLVWIDMEMTGLQPETDRIIEVALVVTDAALATVAQAP
                     VLVVHQDDATLAAMDSWNQSTHGRSGLIDKVKASTLDEATVEQRMLAFLREHVPPRAS
                     PMCGNSICQDRRFLARWMPALEDYFHYRNLDVSTLKELCKRWQPQVAKAWVKQGKHEA
                     LADIYESIDELNYYREHFIGRSVNRVEPA"
     gene            1400..>1730
                     /locus_tag="VHT22_09290"
     CDS             1400..>1730
                     /locus_tag="VHT22_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX3137791.1"
                     /translation="MKFCSHCGSAALASRIPEGDTLPRYVCANCGTIHYQNPKTVVGC
                     LPEWNDRVLLCKRAIEPRLGLWTLPAGFLENGETLETGALRETAEEAGAAVEIGALYT
                     VISLPDIN"
BASE COUNT          346 a          550 c          579 g          255 t
ORIGIN      
        1 ggtgaagtaa gcgttgccgt ggcttgagcg ccgcgagccg tccatcacga acaggccttt
       61 ggccgcgaaa ccgcagcgcg ctagcagtcg ctcgacccgc gcgcgcaacg gctcgtcggc
      121 cagcggctgg aatttgttga acagcggcgc gatgaccgtc ggaaagagca cgagcatcag
      181 cagctggaag gcgacccaga ccagccaggc gtagagccac cacagcgccc cggcccgcgc
      241 catcagccac agcaccaggg tgaggagcgg aaccccgagc agcgcgccga gcagcgcacc
      301 tttcgcgaga tcggcgagcc agaggcgcag cgtcatccgg ttgaagccga accgttgctc
      361 gatgacgaac gtgcgatacc acgagatcgg cagtccgacc agcgcagcca gcaaggtaac
      421 cgcgacgacc agcgcgacgt cctgccacag cgtgccgact ggcaaaaggt cggcgcggag
      481 cgcaagcgcc gcgagtccgc cgcccagggt cagcgccagc aggatgattg cttcgagcac
      541 cgtctccagc atgcccagcc gcgtccgcgc gatcgtgtag tcagcggcct tctgatgcgc
      601 ggccagcgtg atgcgcgtgg cgaaatgcgc gggcggtgca gcgcggttgg cagcaacgtg
      661 ctgcacctgc cggcgcgcga gccagagctg catcgccagc gaaacgccca gcgcgagcag
      721 gaaaatcacg gtgaaccagg gaaaggattg cggcatgcga cgctcgagcg gaccggcgag
      781 tatgcgagaa taagcgctta ttttacgcca agtgaaaata atggcgcagg acgccaatct
      841 gctggtctgg atagacatgg aaatgacggg cttgcagccc gaaaccgacc gcatcatcga
      901 ggtcgcgctg gtcgtcaccg acgcggcgct ggcgaccgtc gcccaggcgc cggtgctggt
      961 cgtgcatcag gatgatgcga ccttggccgc catggactcg tggaatcagt cgacgcacgg
     1021 gcgatcgggc ctgatcgaca aggtcaaggc gtcgacgctc gacgaggcga cggtcgaaca
     1081 gcggatgctc gcgttcctgc gcgagcacgt gccgccacgc gcctcgccga tgtgcggcaa
     1141 ctcgatctgc caggatcgcc gcttcctcgc ccggtggatg ccggcgctcg aagactattt
     1201 tcattaccgc aatctcgacg tgtcgacgct gaaggagttg tgcaagcgat ggcaacccca
     1261 ggtggcgaaa gcctgggtca agcagggcaa gcacgaggcg ctcgccgaca tctacgagtc
     1321 gatcgatgag ctcaactatt atcgcgagca tttcatcggc cggagcgtca atcgcgtcga
     1381 gcccgcctag gtcgatcgag tgaaattctg cagccattgc ggctcggcgg cgctcgcatc
     1441 gcgcatcccc gaaggcgaca ctctgccccg ttacgtgtgc gcgaactgcg gcaccattca
     1501 ttaccagaat ccgaagaccg tcgtcggctg cctgccggaa tggaatgatc gcgtgctgct
     1561 gtgcaagcgc gcgatcgagc cgcggcttgg gctttggacg ctgccggcgg ggtttctgga
     1621 aaacggcgag acgctcgaga ccggcgccct gcgcgagacc gccgaagagg ccggagctgc
     1681 ggtcgagatc ggtgcgctgt acacggtgat cagcctgccg gacatcaacc
//
LOCUS       DATANS010000632         3856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_747684, whole genome shotgun sequence.
ACCESSION   DATANS010000632 DATANS010000000
VERSION     DATANS010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747684"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /gene="glnL"
                     /locus_tag="VHT22_09295"
     CDS             <1..876
                     /gene="glnL"
                     /locus_tag="VHT22_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEX3137792.1"
                     /translation="KALANGASYTEQELEIGIGGKARLHLSGTVSIVDVPGAILLLEF
                     RHIDQQLKIAREERLLEQQQANRDLIRSLAHEIKNPLGGIRGAAQLLERELDRRPLRE
                     YTQVIIGEADRLQALVDRLLTPHRMPKYQSVNIHEILARVGTVVQAEFPRIAILSDFD
                     ISLPEFEGDPEQLTQAIFNVVRNAAQALEGVLGVPQIRLTTRVARGVTLAKRRYRLAL
                     ALLVEDNGPGVPDSIRDRIFFPLVSGREGGSGLGLTIAQTFVTQHNGAIECESVPGHT
                     TFTILLPLEFSRGSA"
     gene            916..2337
                     /gene="ntrC"
                     /locus_tag="VHT22_09300"
     CDS             916..2337
                     /gene="ntrC"
                     /locus_tag="VHT22_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEX3137793.1"
                     /translation="MTRGEPAAAVWIVDDDRSIRWVLEKALSREGIPFRSFASAQEAA
                     QALQHDQPQVLVSDIRMPGETGLELLASIKARHPRLPVIIMTAYSDLDSAVSAFRGGA
                     FEYLPKPFDVDHAVALIRRASADVPATTAPASGVADDAEMLGQAPAMQEVFRAIGRLS
                     HSHATVLVTGESGTGKELVARALHRHSPRASAPFVAINTAAIPKDLLESELFGHERGA
                     FTGAQSARRGRFEQAEGGTLFLDEIGDMPPDLQVRLLRVLSDSEYYRVGGHAPLKANV
                     RIIAATHQNLDERVRQGLFREDLMHRLNVVRLRLPPLRERGEDIAPLARHFLRNSARE
                     LAVEPKQLTDAALKMLAAFEFPGNVRQLENICHWLTVMAPGQRIDAADLPPDVLSASG
                     VSAADSEGNWQQALDRELAQALARGERSVADRLEKEFERTLIVRALAHTGGHRMEAAQ
                     WLGWGRNTLTRKIQELKLDVDKH"
     gene            complement(2368..3255)
                     /locus_tag="VHT22_09305"
     CDS             complement(2368..3255)
                     /locus_tag="VHT22_09305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3137794.1"
                     /translation="MSPGLLRRIRRRLRALGGAGTAFPAGHFYSPVVDPATIARTRVW
                     PDRPDIRGIDFNDASHVQILSELFPRFMRDYDYPPTLPVTPTLASFFGNNPHFSMLDA
                     PALFVLLRAWKPRRIIEVGSGFSTLLMADVNRRFLGREVSISCIDPYPPVFLRHGTDG
                     IGHQVERRVQELPLEHFADLQAGDLLFIDSSHVAKTDSDVNFLFFDVFPRLAAGVLVH
                     LHDIFLPHDYPPDWVLGENRSWNEQYLLRALLMHSGGFEVLFGCSYAFWSYPALVIAA
                     LANPDGAGYGGGSFWMRRR"
     gene            complement(3252..>3856)
                     /locus_tag="VHT22_09310"
     CDS             complement(3252..>3856)
                     /locus_tag="VHT22_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137795.1"
                     /translation="ARLVWAGYYALVLAVSAIVVKSFPPRTATAILALGLALQITDLS
                     PRYVALNGYFRQHFIDEPAQRADSLPSRFWNVAAGHYRTILFVPSLPVPPNFAPIGLF
                     AADHAMRINIGSFARFAIDRVTGARIKREQALAAGRLDQEVLYVLRPPDVASFHAGPD
                     DAIGTVDGFRVLAPGWFRFDDCCTATMPRLAHAAEADRSR"
BASE COUNT          718 a         1243 c         1308 g          587 t
ORIGIN      
        1 aaggcgctgg ccaacggcgc gtcatacacc gagcaggaac tcgagatcgg tatcggcggc
       61 aaggcgcgcc tgcacctttc aggtactgtt tcgatcgtcg atgttcccgg cgcgatcctg
      121 ctgctggaat tccgccacat cgaccagcag ctgaagatcg cgcgcgagga gcgcctgctc
      181 gagcagcagc aggcgaatcg cgacctgatt cgcagcctgg cgcacgagat caagaatccg
      241 ctgggcggca tccgcggcgc ggcgcaattg ctggagcgcg agctcgaccg ccgtccgctg
      301 cgcgaataca cgcaggtgat catcggcgag gccgaccgcc ttcaggcgct ggtcgaccgg
      361 ctgctgacgc cgcaccggat gccgaagtac caaagcgtca acatccacga aattctggcg
      421 cgcgtgggca cggtggtgca ggcggaattt ccgcggatcg cgattctttc cgatttcgac
      481 atcagcctgc ccgagttcga aggcgatccc gagcagctga cgcaggcgat attcaatgtc
      541 gtgcgcaacg cggcgcaggc gctcgagggc gtgctcggcg tgccgcagat ccgcctgacg
      601 acccgcgtcg cgcgcggcgt gacgctggcc aagcggcggt accggctggc gctggcgctg
      661 ctggtcgagg acaacggtcc gggcgttccc gattcgatcc gcgaccgcat cttctttccg
      721 ctcgtatccg gccgcgaggg cggcagcgga ctcgggctga cgatcgcgca aacctttgtc
      781 acccagcaca acggcgcgat cgaatgcgaa agcgttcccg gacatacgac gttcaccatt
      841 ttgctgccgc tggagttcag ccgcggtagc gcgtagattg tgagagatgc gatgctcgct
      901 aatgacccga tcgcgatgac ccgcggcgaa ccggcggcag cagtgtggat cgtcgacgac
      961 gaccgctcga ttcgctgggt gctcgagaag gcgctctcgc gcgagggcat tccgttccgg
     1021 tcgttcgcct cggcgcagga agccgcgcag gcgctgcagc acgaccagcc gcaggtgctg
     1081 gtctccgaca tccggatgcc gggcgaaacc ggcctcgagc tgctggccag catcaaggcg
     1141 cggcacccgc ggctgccggt gatcatcatg accgcgtatt cggatctcga cagtgcggtg
     1201 tccgcgtttc gcggcggcgc gttcgaatac ctgcccaagc cattcgacgt cgatcacgcc
     1261 gtcgcgctga tccgccgcgc cagcgccgat gtcccggcga cgaccgcccc ggcaagcggc
     1321 gtcgccgatg acgccgaaat gctggggcag gcgccggcaa tgcaggaagt ttttcgcgcg
     1381 atcggacgcc tgtcgcattc gcatgcgacg gtgctggtca ccggagaatc gggcaccggc
     1441 aaggagctgg tcgcacgcgc gctgcaccgg cacagcccgc gtgcttctgc gccgtttgtc
     1501 gcgatcaaca ccgcggcaat cccgaaggac ctgctggaat ccgaattgtt cgggcacgag
     1561 cgcggcgcat tcaccggagc gcaaagcgcc cgccgcggcc gtttcgagca ggccgaaggc
     1621 ggcacgcttt tcctcgacga gatcggcgat atgcctccgg acctgcaggt gcgcctgctg
     1681 cgcgtccttt ccgacagcga atattaccgg gtcggcggcc acgctccgct gaaggccaat
     1741 gtgcgcatca tcgcggcgac gcaccagaat ctggacgaac gcgtccggca gggcctgttt
     1801 cgcgaagacc tgatgcaccg gctcaacgtc gtgcgcctgc gcctgccgcc gctgcgcgag
     1861 cgcggcgagg atatcgcgcc gctggcgcgg catttcctgc gcaatagcgc gcgcgagctc
     1921 gccgtcgagc cgaagcagct gaccgatgcg gcattgaaaa tgctggcggc cttcgaattc
     1981 cccggcaacg ttcgccagct cgaaaatatc tgccactggc tgacggtcat ggcgcccggg
     2041 cagcgcatcg atgcggccga tctgccgccg gacgtgctca gcgcatcggg cgtgagcgcg
     2101 gccgacagcg aaggcaattg gcagcaggca ctcgatcgag agctcgcaca ggcgctcgcg
     2161 cgcggcgagc gctcagtggc ggacaggctg gaaaaggaat tcgagcgcac gctgatcgtg
     2221 cgcgcgctcg cccacaccgg cggacaccgg atggaagcgg cgcaatggct gggctgggga
     2281 cgaaacacgc tgacgcgcaa gatccaggaa ctgaagctgg acgtcgacaa gcactgacga
     2341 gccgcagggc aggacctccg cggctgccta gcgccgccgc atccagaagc tgccgcctcc
     2401 gtagccggct ccatcgggat tcgccagcgc ggcgataacg agcgcgggat aactccagaa
     2461 agcgtacgag cagccgaaca gcacttcgaa tccgccgctg tgcatcagca gcgcgcgcag
     2521 caggtattgc tcgttccagc ttcgattctc gccgagcacc cagtccggcg gatagtcgtg
     2581 cggcaggaag atgtcgtgca ggtggaccag cacgcccgcg gcgagccggg gaaagacatc
     2641 gaaaaagaga aaattgacgt cgctgtcggt cttggcgaca tgcgacgaat cgatgaacag
     2701 caagtcgccc gcctgcaggt cggcgaaatg ctccagcggc aattcctgca cgcggcgctc
     2761 gacctggtga ccgatgccat cggtgccgtg tcgcaggaac acaggtggat acgggtcgat
     2821 gcagctgatg ctgacctcgc gtcccagaaa gcgacggttg acgtcggcca tcagcagcgt
     2881 cgaaaagccc gaacccactt cgatgatccg ccgcggcttc caggcgcgca gcagcacgaa
     2941 cagcgcaggc gcgtcgagca tcgagaaatg gggattgttg ccgaagaacg acgcgagcgt
     3001 cggcgtcacg ggcagcgtcg gcgggtagtc gtagtcgcgc atgaagcgcg ggaacagctc
     3061 ggacagaatc tgaacatggc ttgcgtcgtt gaagtcgatc ccgcgaatgt ccggtcgatc
     3121 cggccagacg cgcgtccgcg cgatcgtcgc cggatcgacg actggcgaat agaagtgtcc
     3181 cgccggaaac gccgtccctg cgccgcctaa cgcacgcagt cgccttctta tcctgcgcag
     3241 caggccgggg ctcatcgcga tcgatcggct tccgctgcgt gtgcgagccg cggcatcgtg
     3301 gcagtgcaac agtcgtcgaa acggaaccag cccggcgcga gcacgcggaa tccgtcgacg
     3361 gtgccgatgg cgtcgtcggg tccggcgtga aacgatgcga catcgggagg gcgcaacaca
     3421 tacaggactt cctgatcgag ccggccggcg gcgagcgcct gctctcgctt gattctggca
     3481 ccggtgacgc ggtcgatggc gaaccgggcg aacgagccaa tgttgatgcg catcgcgtgg
     3541 tcggcggcga acaggccgat cggcgcgaaa ttcggcggca ccggcagcga gggaacaaac
     3601 agaatcgtcc ggtaatggcc cgccgcgacg ttccagaagc gcgatggcaa gctgtcggcg
     3661 cgctgcgccg gctcgtcgat gaaatgctgg cgaaagtagc cgttgagcgc gacatagcgc
     3721 ggtgagagat cggtgatctg cagggcgagc ccgagcgcca ggatcgccgt cgcggttcgc
     3781 ggcggaaagc tcttcacaac gatcgcggag acggccagga cgagcgcgta atagccggcc
     3841 cataccaggc gagctg
//
LOCUS       DATANS010000633         2798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_661635, whole genome shotgun sequence.
ACCESSION   DATANS010000633 DATANS010000000
VERSION     DATANS010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661635"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VHT22_09315"
     CDS             <1..745
                     /locus_tag="VHT22_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HEX3137796.1"
                     /translation="HAYDVGVAHDGGYSEYVRVPADWVVRRSPNMTAFEAMTLGTAGF
                     TAAQAIEAMQHNGLTPRSGPVIVNGATGGVGSVAIDVLSRLGYHVVALTGKEKETDYL
                     RSIGASEVVLRSSIDFAKVRPLDKATWAGGIDNLGGEVLAWILSTMKQAGSVASVGLA
                     ADFKLNTTVMPFILRGVNLLGINSSECPMPLRQKLWDRLANEWRPSRVQARVVTIDFA
                     DLPTHFDAFLKGTVRCRTVVRVGADTAGL"
     gene            760..2655
                     /locus_tag="VHT22_09320"
     CDS             760..2655
                     /locus_tag="VHT22_09320"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HEX3137797.1"
                     /translation="MQRYAEAHRRSLADRDVFWRERAALIEWQTPFSEVLDYSRPPFA
                     RWFVGGRTNLCHNAIDRHLATRGAQPALVYISTETGARRSHTDRELADEVNRFAAILE
                     AQGVGKGDRVLIYMPMIPEAVIAMLATVRIGAIHSVVFGGFAAASLATRIDDARPKVL
                     VTADAGMRAGKPVRYKPLVDEALRVASHPPSRVIIVDRGIDSALSSTPGRDLDYATLA
                     AQYAGASVPCAWLESSEPSYILYTSGTTGNPKGVQRDTGGYAVALTASMRDIFCVAPG
                     ETMFTTSDIGWVVGHSYIVYAPLLNGSTTILYEGLPIRPDPGIWWQIVAEHKVRTMFS
                     SPTAIRVLKKQDPSFMKAHDVSSLRYLFLAGEPLDEPTARWAADSLGVAIVDNYWQTE
                     SGWPILSAQLGLAETPRKFGSPSFPVCGYDVKLLDEVTGAEVGTDQKGVLAIAPPLPP
                     GCMTTVWGDDRRFVQTYFETLPGRSVYSTFDWATRDADGYYFVLGRTDDVINVAGHRL
                     GTREIEEAVQAHPGIAEVAVVGVADPIKGQIPVAFAVVKDPSSVATADGAAKMQAEVL
                     STVDRKLGAIARPGAVHFVTLLPKTRSGKLLRRAIQALAEGRDPGDLTTIEDPAALEQ
                     IRAALDL"
BASE COUNT          459 a          915 c          959 g          465 t
ORIGIN      
        1 gcatgcctac gacgtcggcg ttgcgcatga cggcggttac tccgaatacg tgcgcgtgcc
       61 tgccgactgg gtcgtgcgcc gctcgccgaa catgacggcg ttcgaggcga tgaccctcgg
      121 cacggcgggt ttcaccgcgg cgcaggcgat cgaggccatg cagcacaacg gcctgacgcc
      181 acgcagcgga ccggtgatcg tcaacggcgc gaccggcggc gtcggcagcg tcgcgatcga
      241 tgtgctttcc cgactcggct atcacgtcgt tgcgctgacc ggcaaggaaa aggagaccga
      301 ctacctgcga tcgatcggag catccgaagt ggtgctgcgc tcgagcatcg atttcgccaa
      361 agtccgcccg ctcgacaagg cgacctgggc tggcggaatc gacaatctgg gcggagaagt
      421 gctggcctgg atcctctcca cgatgaagca ggcgggctcg gtggcgagcg tcgggctcgc
      481 cgcggacttc aagctcaata cgacggtgat gccgttcatt ctgcgcggcg tgaatctgct
      541 cgggatcaac agctccgagt gcccgatgcc gctgcgccag aagctgtggg accggctggc
      601 caacgagtgg cggccgtcgc gggtgcaggc ccgggtcgtc acgatcgact tcgccgacct
      661 gccgacgcac ttcgatgcct tcctcaaggg cacggtgcgc tgccgcacgg tcgtgcgcgt
      721 cggcgccgac accgccgggc tctgaacctg aagcaagcga tgcagcgcta cgccgaggcg
      781 caccggcgct cactcgccga tcgcgatgtg ttctggcgcg agcgcgcggc gctgatcgaa
      841 tggcagacgc cgttcagcga agtgctcgac tatagccggc cgcccttcgc acgctggttc
      901 gtcggcggcc gcaccaatct gtgccataac gcgattgacc gccatctggc gacgcgcggc
      961 gctcagccgg cgctggtgta catctcgacc gaaaccggcg cgcggcgaag ccacaccgat
     1021 cgcgagctcg ccgacgaggt caatcgcttc gcggcgatcc tggaggccca gggagtcggc
     1081 aagggagatc gcgtgctgat ctacatgccg atgatcccgg aggcggtgat cgcgatgctg
     1141 gccaccgtgc gcatcggcgc catacattcg gtcgtgttcg gcggtttcgc cgccgcgagc
     1201 ctggccacca ggatcgacga cgcgcggccg aaggtcctgg tcaccgccga cgccggcatg
     1261 cgcgccggaa agccggtgcg ttacaaaccg ctagtcgacg aggcgctgcg tgtggcgtcg
     1321 catccgcctt cgcgcgtgat catcgtcgat cgcggtatcg attccgccct aagcagcacc
     1381 cccgggcgcg atctcgacta tgcgacactg gccgcgcagt acgccggcgc gagcgtgccg
     1441 tgcgcctggc tggagtcgtc cgagccttcg tacatcctgt atacctcggg caccaccggg
     1501 aatccgaagg gcgtgcagcg tgacaccggc ggctatgcgg tggcgctcac cgcatcgatg
     1561 cgcgacatat tctgcgtggc tccgggtgag accatgttca ccaccagcga catcggctgg
     1621 gtcgtcgggc attcgtatat cgtctacgcg ccgctgctca acgggtcgac gacgatcctt
     1681 tacgaaggtc ttccgatccg gcccgatccc ggcatctggt ggcagatcgt cgccgagcac
     1741 aaggttcgca cgatgttcag ctcgccgacg gcgatacggg tgctgaaaaa gcaggatccg
     1801 tcgttcatga aggcgcacga cgtgtcgtcg ctgcgctatc tgtttctcgc cggcgagccg
     1861 ctcgacgagc cgaccgcgcg ctgggcggcg gactcgctgg gcgtcgcgat cgtcgacaac
     1921 tactggcaga ccgagagcgg ctggccgatt ctgtcggcgc agctggggct ggccgagacg
     1981 ccgcgcaagt tcggcagtcc atcgtttccg gtgtgcggtt acgatgtgaa gctgctcgac
     2041 gaagtgaccg gagcggaagt cggcaccgac cagaaaggcg tgctcgcgat cgccccgccg
     2101 ctgccgcccg gatgcatgac gacggtctgg ggcgatgacc ggagattcgt gcagacctac
     2161 ttcgagacgc tgcccggccg cagcgtctac tcgacgttcg actgggcgac gcgcgatgcc
     2221 gacggctact atttcgtcct cggccgcacc gatgacgtga tcaacgtcgc cggccaccgg
     2281 ctggggaccc gtgagatcga ggaggcggtg caggcgcatc cgggcatcgc cgaagtcgcg
     2341 gtggtcggcg tggccgatcc gatcaagggc cagatcccgg tggcgttcgc ggtggtgaag
     2401 gatccatcgt cggttgccac cgcggacggt gcggcgaaga tgcaggccga agtgctgagc
     2461 acggtcgatc gcaagcttgg cgcgatcgcc cgccccggcg ccgtgcattt cgtcacgctg
     2521 ttgccgaaga cgcgttcggg caagctgttg cggcgcgcga tccaggcgct ggccgaaggt
     2581 cgcgatccgg gtgacctgac gacgatcgag gacccggcgg cgctcgagca gatccgcgcc
     2641 gcgctggacc tgtagatccg ccgcgcgtcc ggacatcgca accggcgttc gcggcttcgc
     2701 agcgatcgca acagggacgc ggcgggcgcc attgtcgctg ctgctcggaa aaagacaccg
     2761 ttacgcgtaa aatcgatggt ttttcccaag ccgacctt
//
LOCUS       DATANS010000634         1892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_115457, whole genome shotgun sequence.
ACCESSION   DATANS010000634 DATANS010000000
VERSION     DATANS010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115457"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="VHT22_09325"
     CDS             complement(<1..1065)
                     /locus_tag="VHT22_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX3137798.1"
                     /translation="MNKTLLLVDGSSYLYRAFHAIGELRNSKGEPTNAIFGVLNMLRR
                     LHKDIPADYSACIFDAKGKTFRDDIYPEYKANRTAMPDDLGVQIEPLLEAIRAMGWPL
                     LIVDGVEADDVIGTLACQAALQGVRTIVSTGDKDMTQLVNPQVTLVNTMSNETLDEAG
                     VEKKFGVKPERIVDYLSLVGDAIDNIPGVAKVGPKTAVKWLAQYGTLDNIIAHASEVG
                     GAVGDNLREVLDWLPQARGLLTIKCDVELPIKIEELAHTAPDTAKLAELFDRFEFKSW
                     RRELNAAREAADEPVHAPTTAPAAPRAMVERHYETILTEPQLDSWLARVSAAEVTCLD
                     TETTSLDPMQARLVGMSFAVE"
     gene            1107..1826
                     /locus_tag="VHT22_09330"
     CDS             1107..1826
                     /locus_tag="VHT22_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX3137799.1"
                     /translation="MSHPNKVPPRLTTEPGWPARESWRMFGIMSEFFEATERLSNIQP
                     AVSMFGSARVPAGHPYFTLGEQIARRLSDAGFSVISGGGPGMMEAFNKGAYLGKSAAI
                     GLNIELPREQFPNTFQDISLSFRHFFARKVTFVRFSTAYVVLPGGFGTLDELLEALTL
                     VQTGKIQQMPIILVHTPFWRGLVDWLRDRLVAEHMIAPGDVDLVQLVDEPQHVVDAIF
                     AYYEKRGFPSAEAKRESMLTL"
BASE COUNT          334 a          597 c          588 g          373 t
ORIGIN      
        1 ttccacggca aacgacatgc cgaccagccg cgcctgcatc ggatcgaggc tggtggtctc
       61 cgtatcgagg caggtcactt ccgcggcact cacgcgcgcg agccaggagt cgagctgggg
      121 ttccgtcaat atcgtttcgt agtggcgctc gaccatcgcg cgcggcgccg cgggcgcggt
      181 ggtgggcgcg tggaccggtt cgtcggccgc ctcgcgcgcg gcgttcaact cgcggcgcca
      241 gctcttgaac tcgaaacggt cgaacaattc ggcgagcttc gcggtgtccg gggcggtatg
      301 cgcgagctct tcgatcttga tcggcagctc gacatcgcat ttgatcgtca gcaggccgcg
      361 cgcctgcggc agccagtcga gtacctcgcg cagattgtcg cccacggcac cgccgacttc
      421 ggaagcgtgc gcgatgatat tgtcgagcgt gccgtattgc gcgagccatt tcaccgcggt
      481 cttgggcccg actttggcga cacccggaat attgtcgatc gcgtcaccga ccagcgaaag
      541 ataatcgacg atgcgctccg gtttaacgcc gaattttttc tcgacgccgg cttcgtcgag
      601 tgtctcgttg gacatcgtgt tgacgagcgt aacctgcgga ttgacgagct gcgtcatgtc
      661 cttgtcgccg gtggagacga tcgtgcgcac gccctgcagc gccgcctggc acgcgagcgt
      721 gccgatcacg tcgtccgcct cgacgccgtc gacgatcagg agcggccagc ccattgcgcg
      781 gattgcctcg agcagcggct cgatctgcac gcccagatcg tcgggcatcg cggtgcggtt
      841 ggccttgtac tcgggataga tgtcgtcgcg aaaagtcttg cctttggcgt cgaatatgca
      901 ggcgctataa tcagccggaa tatccttgtg cagccgccgc agcatgttca gcacgccgaa
      961 aatcgcgttc gtcggctcgc ctttgctgtt gcgcaattcg ccgatcgcgt gaaacgcgcg
     1021 atacaggtac gacgaaccat ccacgagcag cagggtttta ttcattgcgc atcagcgccg
     1081 taaacacggc aaaagaggag tcgaacatga gccatcccaa caaagtcccg ccacgcctga
     1141 ccactgagcc cggatggccg gcgcgcgaat cgtggcggat gttcggcatt atgtcagaat
     1201 ttttcgaggc gactgagcgg ctctccaaca tccagcccgc agtcagcatg ttcggcagcg
     1261 cgcgcgtgcc cgccgggcat ccgtatttca cgctcggcga acaaatcgcg cgccgtctgt
     1321 ctgacgcggg attcagcgtg atttccggcg gcggtcccgg catgatggag gcgttcaaca
     1381 agggcgccta tctcggcaaa tcggcggcca tcggactcaa catcgaattg ccgcgcgaac
     1441 agtttccgaa cacgtttcag gacatcagcc tgtcgttccg ccatttcttc gcgcgcaaag
     1501 tcacgttcgt gcggttttcc accgcgtacg tcgtgctgcc cggcggcttt ggcacgctcg
     1561 atgaactctt ggaggcgctg accctcgtgc aaaccggcaa gatccagcag atgccgatca
     1621 ttctcgtgca cacgccgttc tggcgcgggc tggtcgactg gctgcgcgac cgtctcgtcg
     1681 ccgagcacat gattgcgccc ggcgacgtcg acctcgtgca actggtcgac gagccgcagc
     1741 acgtggtcga cgcgatattc gcctactacg aaaaacgcgg gttcccgtcg gccgaagcga
     1801 agcgcgaatc catgttgacg ctatagcgac ggtggcggga gcagtcggct gccgggtgcc
     1861 cgcgctttat atttccggcc gaaaagccga ta
//
LOCUS       DATANS010000635         2300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_238898, whole genome shotgun sequence.
ACCESSION   DATANS010000635 DATANS010000000
VERSION     DATANS010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238898"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1659
                     /locus_tag="VHT22_09335"
     CDS             <1..1659
                     /locus_tag="VHT22_09335"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX3137800.1"
                     /translation="PEVAVCRDVAAMPKRKATAQRKPERLRHLQFEGISIRPGEPVAD
                     ARQAVAAVRKILKAEHLAWRIQRYGKRARSVLVLPPPGSPSPSPAQAWELTYRLRARR
                     DVMHVEPAFVLPAVGPYRAPARRSAAGGTSAHLPGSDDNDWALKLCRVSEAWNIPTPN
                     GPQGKGSIVAHPDTGYTRHPELIRSDILFAKGYDFVADTPDALDPLDPPNAGHGTSTG
                     SVILSDIGPAGAIHVTGVAPQASLIPLRVSSSVVHFSWTRLCAALYRAIDDDAHVVSM
                     SLGGPWGGFALHDAVRDAVDAGLMLVAAAGNGWPFVVYPARYDEVIAVAACNVDKQRW
                     AGSASGEAVDITAPGESVWRARTQSDGTFDVDRSSGTSYAAAHVAGIGALWLAHHGRA
                     NLVTKYGSARIASVFKEMLTTAGHAGTPLPWPTTALGAGIANAQSLLEAALPATVHAA
                     GMAVRAGGRTPVENDFDRIASYFPRADPSRVRAWLLGMFGVKERELATRLASFADESA
                     FHLAADPNAYAATHAALAGRRGAVAPRPARLLRRASPSLKRQLA"
     gene            1688..>2300
                     /locus_tag="VHT22_09340"
     CDS             1688..>2300
                     /locus_tag="VHT22_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137801.1"
                     /translation="MTILFIPNDPEAKKSPPARKIAPAPARRTGKTTFDFGKLPQLKV
                     WPADSPEFLVWQCREAVMRALAMWEKIAGPLKHWQGNAQRKKLKVNIDEGSDLNAYYD
                     RQSLGYYHFRSKKTKLLRRFAASTDVVSHECGHALLDAVRPELWDSNFPEPNAFHEAF
                     GDCVALLTALADLETRKALLKQVPKLAGANFVETLIESLANGLR"
BASE COUNT          397 a          783 c          760 g          360 t
ORIGIN      
        1 ccggaagtcg cggtctgtcg agacgtagca gcgatgccca aacgaaaagc cacagcgcaa
       61 cgcaagcccg agcggctgcg ccatttgcaa ttcgagggta tcagcatccg gcccggcgag
      121 ccggtcgccg acgcacgaca agcggtcgcg gccgttcgca agatcctcaa agcggagcat
      181 ctcgcctggc ggatccagcg ctatggcaag cgggcgcgca gcgtgctcgt gttgccgccg
      241 cccggatcgc cgagcccctc gccggctcag gcctgggagc tcacctaccg gctgcgcgca
      301 cggcgcgatg tcatgcacgt cgagcccgca ttcgttctgc cggccgtcgg accgtatcgc
      361 gcgccggcgc ggcgcagtgc agccggcggc acatccgctc acctgccggg cagcgacgac
      421 aacgattggg cgttgaagct ttgtcgcgta tccgaggcct ggaatattcc gacgcccaac
      481 gggccgcagg gcaaaggctc gatcgtcgcg catccggaca ccggctacac gcggcatccc
      541 gagctcattc gctcggacat cctcttcgcc aaggggtatg actttgtcgc ggacacgcct
      601 gacgcgctcg acccgctcga tccgccgaac gcaggtcacg ggaccagcac cggcagcgtg
      661 atcctgagcg acatcggtcc cgccggcgcg atccatgtca ccggcgtcgc gccgcaggcg
      721 tcgctgattc cgttgcgggt gagcagcagc gtggtccatt tctcctggac gcgcctttgc
      781 gccgcgctct atcgcgccat cgacgacgac gcgcatgtcg tctcgatgag cctcggcggc
      841 ccgtggggcg gattcgcgct gcacgacgcc gtccgcgacg ccgtcgatgc cgggctgatg
      901 ctggtagccg ccgccggcaa cgggtggccg ttcgtcgtct atccggcgcg ctacgacgaa
      961 gtcatcgcgg tggcggcatg caacgtcgac aagcaacggt gggccggctc ggcaagcggc
     1021 gaggcggtcg acatcacggc acctggcgaa tcggtctggc gggccaggac ccaaagcgac
     1081 ggcacattcg acgtcgatcg cagctcgggg acttcgtacg ccgccgcgca tgtcgccggg
     1141 atcggggcgt tgtggctcgc gcaccacggg cgcgcaaacc tggtgacgaa atatggcagc
     1201 gcccggatcg cttcggtgtt caaggaaatg ctgaccaccg ccggtcacgc gggcacgccg
     1261 ctgccgtggc cgacgactgc gctgggcgca ggcatcgcca acgcgcaatc gctgctagag
     1321 gcggcgctcc ctgccaccgt ccacgccgcc ggcatggcgg tgcgcgccgg aggcaggacg
     1381 ccggtcgaaa acgatttcga tcggatcgcg tcgtatttcc cgcgcgccga cccgtcgcgc
     1441 gtccgcgcct ggctgctcgg aatgttcggc gtgaaggaac gcgagctcgc caccaggctc
     1501 gcctcgttcg ccgacgagag cgcattccat ctcgccgccg atcccaacgc ctacgccgcg
     1561 acgcatgccg cgctggcggg gcgtcgcggc gcggttgcgc ctcggccggc caggcttctg
     1621 cggcgagcct cccccagcct gaagcgacag ctggcgtaat cgaatacctt cgatcaggag
     1681 tttagccgtg actattctgt tcatcccgaa tgatccggaa gcaaaaaaat cgccgcccgc
     1741 acgcaagatc gcgccggcgc ccgcacgcag gacgggcaag accaccttcg acttcggcaa
     1801 actgccgcag ctcaaagtgt ggccggcgga cagcccggag ttcctcgtgt ggcagtgccg
     1861 cgaggcggta atgcgtgcac ttgccatgtg ggaaaagatc gccgggccgc tcaagcactg
     1921 gcaggggaat gcgcaacgga agaagctgaa ggtcaacatc gacgagggct cggatctcaa
     1981 tgcttattac gacaggcagt cgctcgggta ctatcatttc aggagcaaga agaccaagtt
     2041 gctgcgacgg ttcgccgcga gcaccgatgt cgtctcgcac gagtgcggac acgcgctgct
     2101 cgatgcggtc cggcccgagt tatgggacag caactttccc gaacccaatg cattccacga
     2161 ggcgttcggc gactgcgtcg cgctgctgac ggcgcttgcc gacctcgaaa cgcgcaaggc
     2221 cttgctcaag caggtgccga agctcgccgg cgccaatttt gtcgagacgc tgatcgaatc
     2281 gctggcgaac ggtctgcgtg
//
LOCUS       DATANS010000636         3130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_617134, whole genome shotgun sequence.
ACCESSION   DATANS010000636 DATANS010000000
VERSION     DATANS010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617134"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VHT22_09345"
     CDS             <1..461
                     /locus_tag="VHT22_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HEX3137802.1"
                     /translation="GRLLVERGELPLARASMQGIKQWGREHPEALPPLLAENPSFVFF
                     RVIEPDPKAAIDGPIGTLGVPLLRERTIAVDPTIVPLGAPVFLATTYPLSDRPLQRLV
                     LAQDTGGAIKGVLRADFFWGFGDDAGREAGRMKQAGRMWLLWPKGAPLPY"
     gene            complement(483..1505)
                     /locus_tag="VHT22_09350"
     CDS             complement(483..1505)
                     /locus_tag="VHT22_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011004756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3137803.1"
                     /translation="MILTGRLGRLGVACAAVLAFAMSQAAYGEVAEVRISHGYGVLYL
                     PLMVMASEKLVEKHAAAAGLPAVKASYVVLDGGNVINDAMLSGALDIASSGVPGFLTL
                     WAKAKDNPAQEVRGLSGMSSSSMYLMTRNPSVKSLKDFTDKDRIAVPGIKTSLPAVLL
                     HMAAAKEFGDANYAKLDPITVGLPHPEALTVLLSGRGEIDSHMASPPFSYIEDATPGL
                     HRVFNSVDLLGNITLDMTYTSRRFFEANPKLCAAFIAAMNEANALIANDKRRAAEIYI
                     AVSRQKSSSDEVSRILSDPNSKFSTIPDGTMRYAEFMSRVGTIKAKPASWKELFFPPL
                     HALPGS"
     gene            complement(1522..2496)
                     /gene="lipA"
                     /locus_tag="VHT22_09355"
     CDS             complement(1522..2496)
                     /gene="lipA"
                     /locus_tag="VHT22_09355"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX3137804.1"
                     /translation="MSVVQPPDPLANAAGVKHKGDAKTARIPIKIVPAERLKKPDWIR
                     VRAPSSPRFHEIKRILREHKLHTVCEEASCPNIGECFGKGTATFMIMGDICTRRCPFC
                     DVGHGRPLPLDADEPANLAATIAALKLAYVVITSVDRDDLRDGGAQHFVDCIRAVRER
                     SPATRIEVLVPDFRGRLDRALDVLAQAPPDVMNHNLETVPRLYKQARPGSDYANSLRL
                     LQAFKARFPDIPTKSGLMVGLGETDEEIVAVMRDMRAHDIDMLTIGQYLQPTVSHLPV
                     LRYVHPDGFAMFEREAYALGFEHAAVGALVRSSYHADAQAHGVLEAAG"
     gene            complement(2493..3104)
                     /gene="lipB"
                     /locus_tag="VHT22_09360"
     CDS             complement(2493..3104)
                     /gene="lipB"
                     /locus_tag="VHT22_09360"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX3137805.1"
                     /translation="MGQTDYASTWRAMQAFTNARAPETPDEIWLTEHPPIYTLGLAGR
                     REHLHRDNGIPVLKVDRGGQITYHGPGQLIVYLLIDIGRRRLGVRQLVGAIEDSVIQL
                     LDSYGVAATARRGAPGVYVQRRDSEAKIAALGLKIRNGRSYHGLALNIDMDLAPFADI
                     DPCGYRGLAVTRLRDLGIDAPRAEIESRLVAALDAAIGAASDR"
BASE COUNT          526 a         1000 c         1054 g          550 t
ORIGIN      
        1 tcgggcgctt gctggtcgag cgcggcgaat tgccgctggc acgggcgtcg atgcagggga
       61 tcaagcagtg gggccgcgag catccggaag cattgccccc gttgctggcc gagaatccga
      121 gctttgtgtt ctttcgcgtg atcgagccgg acccgaaggc ggcgatcgac gggccgatcg
      181 gaactctcgg cgtgccgttg ctgcgagaac ggacgattgc cgtcgatccg acgatcgtac
      241 cgctgggcgc gccggtattt ctggcgacga cttatccgct ttcggaccgg ccgctgcagc
      301 gcctggtact ggcgcaggac accggcggcg cgatcaaggg agtgttgcgc gccgatttct
      361 tctggggctt tggcgacgac gccggtcgcg aggcaggccg catgaagcag gcgggcagga
      421 tgtggctgtt gtggccgaag ggcgcgccgc ttccgtattg agtgaacgcc gcggaagacg
      481 cgtcagctgc cgggcagcgc gtgcagtggc gggaaaaaaa gctccttcca ggacgccggc
      541 ttcgccttga tcgtcccgac acgcgacatg aattcggcat agcgcatcgt gccgtcgggg
      601 atcgtggaaa atttggagtt ggggtcgctg aggatgcgcg agacttcgtc cgacgaagac
      661 ttctgcctgg atacggcgat gtaaatctcg gccgcgcggc gcttgtcgtt cgcgatcagg
      721 gcattggcct cgttcatcgc cgcgatgaac gccgcgcaga gcttcgggtt ggcttcgaaa
      781 aagcgccggc tggtgtaggt catgtccagc gtgatgttgc ccagcaagtc gaccgagttg
      841 aacacgcgat gcagccccgg ggtcgcgtcc tcgatgtagg aaaacggcgg cgaggccatg
      901 tggctgtcga tctcacccct gccggaaagc agcaccgtca gcgcctccgg atgcggcagt
      961 ccgacggtga tcggatcgag cttcgcatag ttggcgtcgc cgaactcctt cgccgcggcc
     1021 atatgcagca ggacggcggg aagcgaggtc ttgatgccgg ggacggcgat gcggtccttg
     1081 tcggtaaagt ccttcaacga tttgacgctt ggattgcgcg tcatcagata catcgatgac
     1141 gagctcatgc cgctcaggcc gcggacctcc tgcgcgggat tgtccttggc ctttgcccac
     1201 aaggtgagga atccgggcac accgctcgac gcgatgtcga gcgcccccga aagcatcgcg
     1261 tcgttgatca cgttgccgcc gtcgagcacg acgtagctcg ccttgacggc gggcaggcct
     1321 gcggcagcgg cgtgcttctc gacgagcttc tcgctcgcca tcaccatcaa cggcagatac
     1381 agcacaccgt agccgtgcga aatgcggact tcggcgactt cgccgtacgc cgcttgcgac
     1441 atcgcgaacg ccaacacggc agcgcaagcg accccgaggc gccccagcct gccggtcagg
     1501 atcattctct tacgcgccgc ttcaaccggc ggcttcgagc acgccatgtg cctgggcgtc
     1561 ggcgtgatac gacgatcgca ccagcgcgcc gacggcggcg tgttcgaagc ccagcgcata
     1621 ggcttcgcgc tcgaacatcg cgaagccatc cggatgcacg tagcgcaaca ccggcagatg
     1681 tgaaacggtc ggctgcagat actggcctat ggtcagcatg tcgatgtcgt gcgcgcgcat
     1741 gtcgcgcatc accgccacga tttcttcgtc ggtctcgccc aagcccacca tcagtcccga
     1801 ctttgtcgga atatccggaa agcgcgcctt gaatgcctgc agcagccgca gcgaattcgc
     1861 gtagtcggat ccgggccgcg cctgcttgta gagccgcggc accgtctcga ggttgtggtt
     1921 catcacgtcg ggcggcgcct gcgccaggac gtccagcgcg cggtcgagcc tgccgcgaaa
     1981 gtcgggcacc agcacctcga tccgcgtcgc cggcgagcgt tcgcgcaccg cgcgtatgca
     2041 gtccacgaaa tgctgcgcgc cgccgtcgcg aaggtcgtcg cggtccacgc tcgtgatcac
     2101 gacgtaggcg agcttcagcg ccgcgatggt cgcggcgaga ttggccggct cgtcggcatc
     2161 caatggcagc ggccgcccgt gcccgacatc gcagaacggg cagcggcgcg tgcagatatc
     2221 gcccatgatc atgaacgtcg cggtgccctt gccgaaacac tcgccgatgt tcggacacga
     2281 agcctcttcg cagaccgtgt gcagcttgtg ctcgcgcagg atgcgcttga tctcgtggaa
     2341 gcgcggcgac gaaggggcac gcacgcggat ccagtccggc ttcttcaatc gctcggccgg
     2401 cacgatcttg atgggaatgc gcgcggtctt cgcgtccccc ttgtgcttga cccccgcggc
     2461 gttggccagc gggtccggcg gctggacgac gctcatcggt cggacgccgc gccgatcgcg
     2521 gcgtcgagcg ccgcaaccag ccgcgactcg atttcggcgc gcggcgcgtc gatgccgaga
     2581 tcgcgcaacc gcgttaccgc gaggccgcgg tacccgcagg gatcgatatc ggcgaacggg
     2641 gcaagatcca tgtcgatatt gagcgccagg ccgtgatagc tgcgcccgtt cctgattttg
     2701 aggcccagag ccgcgatctt ggcctccgaa tcgcgccgct ggacatagac accgggcgcg
     2761 ccgcggcggg ctgtcgcagc aacgccatac gaatcaagca attgtatcac gctgtcctcg
     2821 atcgccccga ccagctgccg cacacccaag cgtcggcggc cgatgtcgat aagcaggtag
     2881 acgatcagtt gtccgggacc atggtaggtg atctggccgc cgcgatcgac cttgagcacc
     2941 gggatgccgt tgtcgcggtg caagtgctca cgacggccag cgaggcccag cgtgtagatc
     3001 ggcggatgct cggtcagcca gatttcgtcc ggcgtttcgg gtgcgcgagc gttggtgaat
     3061 gcctgcatcg cgcgccaggt cgaggcgtaa tcggtttgcc ccaggcgacg gacccggcaa
     3121 ggctcgccgg
//
LOCUS       DATANS010000637         2047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_452681, whole genome shotgun sequence.
ACCESSION   DATANS010000637 DATANS010000000
VERSION     DATANS010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452681"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1559)
                     /locus_tag="VHT22_09365"
     CDS             complement(147..1559)
                     /locus_tag="VHT22_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX3137806.1"
                     /translation="MGNGWKKAGLIGIGAVLGLLLSLNFSALAEREAKLPIPYEDLQL
                     FSAVFGRIKSDYVEVVPDDKLIREAINGMVHGLDPHSDFLDADAFKELQINTQGKFGG
                     LGIEVGAEDGLIKVISPIEDTPAFRAGIKSGDLIVKIDDLSTRGLPLSKAVEKMRGKP
                     GTSIVLTIARKDAETPLTFTLLREIINFRSVRAKLIEPGYGYIRVVQFQEHTGEDVAK
                     SIRDLYKQGELTGLVLDLRSDPGGLLNVSVGVAAAFLPADSLVVYTDGRTSDARMRLS
                     STKGDYLRGRSDDYLKDLPAAVKRVPMVVLVDGGTASASEIVAGALQDHKRAVVMGTQ
                     TFGKGSVQTILPLGPNAGLKLTTARYYTPSGRSIQAKGIEPDIAVDDGRDYRLREANL
                     DHHLLDGKTPIASADGAATLSIKTPVEKTQENPKKGEAAQPRLEFGSTDDFQLKQALN
                     RLKGLPVIASTKAVAAVAPQ"
BASE COUNT          328 a          692 c          631 g          396 t
ORIGIN      
        1 cgcgtcgacg cccaactcgg gcagcagcac gtgccggctg tagcgcagca gctgggcgtc
       61 gtccatcgta gttttccgtt gcccggtcgt tcgccagcaa aaacgcccgg cgagccgggc
      121 gtcttgcgtt gccagaacct cgttgcttac tgcggcgcta ccgcggccac cgccttggtc
      181 gatgcgatga ccggaagacc cttcaggcgg ttaagcgcct gcttgagctg aaaatcatcg
      241 gtcgagccga attccagacg cggctgcgca gcctcgccct tcttcggatt ttcctgcgtc
      301 ttttcgaccg gcgtcttgat cgaaagcgtc gcagcgccat cggcgctggc gatcggcgtc
      361 ttgccgtcga gcaggtgatg atcgagattg gcctcgcgca gccggtagtc gcgtccgtca
      421 tcgaccgcga tgtcgggctc gatgcccttc gcctggatcg agcggccgct cggcgtgtag
      481 tagcgcgccg tcgtgagctt cagccccgcg ttcggcccca acggaaggat ggtctgcacc
      541 gaacccttgc cgaaggtctg cgtgcccatc accacggcgc gcttgtggtc ctgcagggcg
      601 ccggcgacga tctccgaagc cgacgcggtg ccgccgtcga ccagcacaac catcggcacg
      661 cgcttgaccg ctgcgggcag gtccttcaga tagtcgtcgc tgcgccctcg cagatagtcg
      721 cccttggtcg acgaaaggcg catgcgcgca tcggacgtgc ggccatcggt gtacacgacc
      781 agcgaatccg ccggcaggaa cgccgcggcg acgccgaccg agacattcag caagccgccc
      841 ggatcgctgc gcagatcgag gaccagcccg gtgagctcgc cctgcttgta caaatcgcgg
      901 atcgacttgg cgacgtcctc gccggtatgt tcctggaact gcacgacgcg gatgtaaccg
      961 tagccgggct cgatcaattt ggcgcgaacg ctgcggaaat tgatgatctc gcgcaacaag
     1021 gtgaatgtca gcggcgtctc ggcgtccttg cgcgcgatcg tcagcacgat cgacgtgccc
     1081 ggcttgccgc gcattttctc gaccgccttc gacagcggca gcccgcgagt tgaaaggtcg
     1141 tcgatcttga cgatcaggtc tcctgacttg attccggcgc ggaaggccgg cgtgtcctcg
     1201 atcggcgata tcaccttgat cagtccgtcc tcggcgccga cttctatccc gaggccgccg
     1261 aacttgccct gggtattgat ctgcaattcc ttgaacgcgt cggcgtccag aaagtccgag
     1321 tgcgggtcga gcccgtggac catgccgttg atcgcttcgc ggatcagctt gtcgtccggc
     1381 acgacctcga cgtagtcgct cttgatgcgg ccgaacacgg ccgagaagag ctgcagatcc
     1441 tcgtagggga tcggaagctt tgcctcgcgc tcggccaacg ccgagaagtt gaggctgagc
     1501 agcagcccca gcaccgcacc gataccgatc agtcccgcct ttttccagcc attacccatg
     1561 tttgcctcgt cgtgtcgcga ttgcgcgctt ggacgcaacc gctcggtatg ccggtgttcg
     1621 gcggcccgat ggtctatcga tgtcgctgcg ctcgcttccc tgagcgcggc cctctcggcc
     1681 ggcgggttgc tgcgcaatcg ttgcaggatc atggcgcgat ccacattgcc ggatcgaccg
     1741 cctctccctg gattcgtatt tcaaagtata cgccggtttc ctggccgcca ccgctgcgcc
     1801 ggcctgcgcc actatccggc cggcgtcgag ctttgcgccg accggcgaca acagtgactc
     1861 gttgttgccg cagaggctca tatagccgcc tccgtgatcg ataatcaaga ggtttccgaa
     1921 tccgcgcagc cattcggcgt agacgacctg accggcggcg accgctcgca cggcctgtcc
     1981 tccggggcag cagaccaacg atcccttcca gcgcgctccg ctcccgctgc gtggactgcc
     2041 aaaccgg
//
LOCUS       DATANS010000638         2535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_747870, whole genome shotgun sequence.
ACCESSION   DATANS010000638 DATANS010000000
VERSION     DATANS010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747870"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VHT22_09370"
     CDS             complement(<1..651)
                     /locus_tag="VHT22_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012406739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3137807.1"
                     /translation="MNFTDIFIRKPVLATTISLLILVLGVRSLLGLSVRQYPKTQNAV
                     VTISTAYYGADAATVAGFITQPLEGAIAQAQGIDYLSSTSTSGVSSITATLRLNYDAN
                     RALTEINTQVNSVRNQLPPEAQQPVLNVQVGQQIDAMYMGFFGQALPNNNVTDYLVRV
                     VKPKLDSIEGVQVAEILGARTFALRAWLDPARMAAYGVTATDIRTALGNNNYLAALG"
     gene            complement(651..1784)
                     /locus_tag="VHT22_09375"
     CDS             complement(651..1784)
                     /locus_tag="VHT22_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3137808.1"
                     /translation="MKKRMIIMLVLVGVLLGGIVGFNVFKGYMMQKYMASAPVPPATV
                     SAAKVDYQEWQPQLNAIGTLRAIRGVDVTTEVAGLVRSINFKSGDEVKAGQVLAQLND
                     ETEIAQLNSLQAAADLAATVYERDKQLLAAEVASKAQVDADAGDLKSKQALVAQQAAL
                     IRKKTLRAPFTGKLGISTVNPGQYLNPADKLVTLQTIDPIYVDFNLPQQQLPQISVGQ
                     KVTLTSDAYPGQTFIGKVNAINPKIDPATRNVQIEATVPNPKRELLPGMFASVRVDRG
                     AQQRYLTLPQTAITFNPYGDTVFVVKTSDQKDAKGNQQLTAQQVFVTTGPTRGDQVAI
                     LKGIEPGTQVVTSGQVKLKNGTPLVIDNSVQPTNNPNPTVQEK"
     gene            complement(1860..>2535)
                     /locus_tag="VHT22_09380"
     CDS             complement(1860..>2535)
                     /locus_tag="VHT22_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX3137809.1"
                     /translation="LAGRVPSDGGIPTFDLAALTLPQDLPLSLPSALARQRPDVRAAE
                     ALLHQASAQIGVATANQYPQINLTGSFGAQSTELHSLFAGPSIWSIGAGLLQPLFHAG
                     ELEHKRRAAVAAYDQAAAQYRETVLLAFQNVADALRALEADARTLKAQSDAEALARET
                     LNLTQRQFQLGATNYIALLDAQRQFNQARFLLVQAQANRYADTAALFQALGGGWWNGD
                     AAAPPA"
BASE COUNT          394 a          789 c          848 g          504 t
ORIGIN      
        1 gccgagggcc gcgagataat tgttgttacc cagcgccgtg cgtatgtcgg tcgcggtgac
       61 gccgtaggcc gccatccgtg ccgggtcgag ccaggcccgc agcgcaaagg tccgcgcgcc
      121 gaggatttcg gcgacctgca cgccttcgat cgaatccagc ttcggcttga cgacgcggac
      181 caggtagtcg gtgacgttgt tgttgggtag cgcctgcccg aaaaagccca tgtacatcgc
      241 gtcgatctgc tgcccgacct gcacgttaag caccggctgc tgcgcctcgg gcggcaactg
      301 gttgcgcacc gagttgacct gcgtgttgat ttcggtcagc gcgcggttgg cgtcgtagtt
      361 gaggcgcaaa gtcgccgtga tcgacgacac acccgacgtg ctggtcgacg acaggtagtc
      421 gataccctgc gcctgcgcga tcgcgccttc gagcggctgg gtgatgaagc cggcgacggt
      481 cgccgcgtcg gcgccgtaat aggcagtgct gatcgtgacg acggcattct gtgtcttcgg
      541 gtactgccgt accgataggc ccaacaacga tcggacaccg agcacaagga tcagaaggct
      601 gatcgtcgtc gcaagcacgg gcttgcgaat gaagatgtcg gtgaagttca ttatttttcc
      661 tgcacggtcg gattggggtt attcgtcggc tgaaccgaat tgtcgatgac cagcggggtg
      721 ccattcttga gcttcacctg gccgctggtg acgacctgcg tgcccggctc gatgcccttc
      781 aggatcgcca cctggtcgcc gcgggtcgga ccggtcgtca cgaacacctg ctgcgcagtc
      841 agttgctggt tgcccttcgc gtccttctgg tccgacgtct tgacgacgaa tacggtatcg
      901 ccataagggt tgaacgtgat cgccgtctgc ggcaaggtca aatagcgctg ctgcgcaccc
      961 cggtcgaccc tgacgctggc gaacatgccg ggcagcagct cgcgcttggg gttcggcacc
     1021 gtcgcttcga tctgcacgtt gcgcgtcgcg ggatcgatct tcgggttgat cgcattcacc
     1081 ttgccgatga aagtctgccc cgggtaggca tcgctggtca gcgtcacttt ctgtcccacc
     1141 gagatctgcg gcagctgttg ctgcggcaga ttgaagtcga cgtagatcgg gtcgatcgtc
     1201 tgcagcgtga ccagcttgtc ggccgggttc aggtactgcc ccggattgac ggtgctgatg
     1261 cccagcttgc cggtgaacgg ggctcgcagc gtcttcttgc ggatcagcgc cgcctgttgc
     1321 gcgactagcg cctgcttgct cttcaggtcc ccggcatcgg cgtcgacctg ggccttgctg
     1381 gcgacttccg cggcgagcag ttgcttgtcg cgctcgtaga ccgtcgccgc gagatcggcc
     1441 gccgcctgca gcgaattgag ttgcgcgatc tcggtctcgt cgttgagctg ggcgagcacc
     1501 tggccggcct tgacctcgtc acccgatttg aagttgatgc tgcggaccag tcctgcgacc
     1561 tcggtggtta cgtcgacgcc gcgtatcgcg cgcagcgtgc cgatcgcatt gagctgcggc
     1621 tgccattcct ggtagtcgac cttggccgcg ctgaccgtcg ccggaggcac cggcgcggag
     1681 gccatgtatt tctgcatcat gtagcccttg aatacattga agcccacgat gccgccgagc
     1741 agcactccga cgagcaccag catgatgatc atgcgtttct tcattgaagg cttcatcgca
     1801 aacttctccg ttcgaatgtt cgagtaggaa tcggtggtga gtttgaatcg ccggcgcgat
     1861 caggccggag gcgcggccgc gtcgccgttc caccagccgc cgccgagcgc ctggaacagc
     1921 gccgcggtgt cggcgtagcg gttcgcctga gcctggacca acaagaaacg cgcctggttg
     1981 aactggcgct gcgcatcgag cagcgcgata tagttcgtgg cgccgagctg gaattgccgt
     2041 tgtgtcaggt tcagcgtctc gcgggcgagc gcttcggcgt cggactgcgc cttcaacgtt
     2101 cgcgcatcgg cctctagcgc gcgcagggca tcggccacgt tctggaacgc gagcagcacc
     2161 gtctcccgat actgcgccgc ggcctggtcg taggccgcga cggcggcgcg gcgcttgtgc
     2221 tcgagctcgc cggcgtggaa cagcggttgc agcaatccgg cgccgatgct ccatatgctc
     2281 gggccggcga aaagactatg cagctcggtc gactgcgcac cgaagctgcc ggtcaggttg
     2341 atctgcggat actggtttgc cgtggcgacc ccgatctgcg cgctggcctg gtgcagcagc
     2401 gcctccgcgg cgcggacatc gggccgctgc cgcgccagcg ccgacggcag cgagagcggc
     2461 aagtcctgcg gcaaggtcaa ggcggccaga tcgaaggtgg gaatgccgcc gtcgctgggg
     2521 acccggccgg cgagt
//
LOCUS       DATANS010000639         3619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_705844, whole genome shotgun sequence.
ACCESSION   DATANS010000639 DATANS010000000
VERSION     DATANS010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705844"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VHT22_09385"
     CDS             <1..199
                     /locus_tag="VHT22_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3137810.1"
                     /translation="FIDFIVSDEGQKLALSMGYIPAKASVGTPSWLPPGTVIKLMPTD
                     IGNVVQNNSANLKRFSELFGN"
     gene            223..1926
                     /locus_tag="VHT22_09390"
     CDS             223..1926
                     /locus_tag="VHT22_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013963119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX3137811.1"
                     /translation="MADPAGWRERAEETVLLWVLVAIVATLSLLPLARLLFEGIAPGG
                     RFSTAALSSVLSSATTWTAARHSLVTACGGTALAVLIGATVAVLVALTDIRGRNAFVF
                     CFVLPLMIAPQVVALAWLQLFGPSSTLLKMIGLAPAMGSRNPLYSAHGIILLLGVQYA
                     PLVFLTLRAGLRTLPQELLEAGLAAGAKPPTILRTIVLPLMTPPLVAGIALCFVSCLG
                     NFGIPAFLGIPGNYLVLPTLIYQRLAGLGPSVLSEVAVLSCLIGFIAVLGIFLQDLML
                     RRRDFRITTTSNAMRPFELARWRPAVEIALWILTIVVLVLPLVALLLSSLVPAAGVPL
                     TAKSATWSNYDYVLFDHAAAKRALVNSFSLSATAAIVIVLVAVPLAYFLVWRRSRLLR
                     VLNLAAEMPYALPGVVLAIAAILVFLKPLPVLGLTLYNTVWIILFGYLARFLVLGLRP
                     VIGGYYQLDRTLEEAAQIAGARLLRRLATIILPLVAPMATAGALLVFLTAFNELTVSA
                     LLWSSGAETLGVVVFSFQQGGDSTYAAALAMLTVAVTIALMLSTLLTGKPARGVLPWR
                     D"
     gene            1914..3068
                     /locus_tag="VHT22_09395"
     CDS             1914..3068
                     /locus_tag="VHT22_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3137812.1"
                     /translation="MARLIVDCVTKTFGALHALDQVSLDVGDGEFMAILGPSGCGKTT
                     LLRQIAGFDKLDGGRIVIGETVVSTVTQHVPPERRRIGIVFQSYALWPHMTVAENVAY
                     GLTVAGVRDPERTRRVDAALTLVELEGYAERRPSMLSGGQRQRVALARCLVTEPSLVL
                     LDEPLANLDVHLRASMEEEFARFHARTGTTMVYITHDQAEAMALADRIAVMDRGRLLQ
                     VATPSQLYREPADASVAAFIGEGIVVPAEVIDVDSRGRCRVDLFGLSVSMRCPLAQTP
                     TRAARVCLRAANLAIVGAGVPGIGARVVRSVYQGGHFRVEAQLDHADGVLVHLAAAEP
                     CRLAPGDAIRIGIDDGWVLPETGEIVDSTAPESADSRRFDGGTVATGADG"
     gene            3075..>3619
                     /locus_tag="VHT22_09400"
     CDS             3075..>3619
                     /locus_tag="VHT22_09400"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3137813.1"
                     /translation="MRLHLYGGFGEKGRTCLGVEVDGYRLLLDAGVKTSARGADYYPA
                     ITTDELGATDAIVITHAHEDHVAALGWCIAGGFRGRLLMTAETGREVDLFLEAYADAS
                     HRGAAREFAREELPVGASTALGPLQLIAGRSGHVSGGVWCTVADGRRTLGYCGDVVPA
                     SEVFAMDPLPRCDAVVLDASYG"
BASE COUNT          492 a         1218 c         1210 g          699 t
ORIGIN      
        1 attcatcgac ttcatcgtgt cggacgaagg gcagaagctt gcgctgtcga tgggctacat
       61 cccggccaag gcaagcgtag gtacgccgtc gtggctgccg cccggaacgg ttatcaagct
      121 catgccgacc gacatcggca acgtggtgca gaacaacagc gccaatctga agcgtttctc
      181 cgagctcttc ggtaactgat ccccagttgc cggcgcgcca cgatggcgga ccccgcaggc
      241 tggcgcgagc gcgccgagga gaccgtgctg ttgtgggtgc tggtcgcgat cgtcgcgact
      301 ctgtcgctgt tgccgctggc gcggttgctg ttcgagggca tcgcgcccgg tggccggttc
      361 tcgaccgccg cgctctcgag cgttctgtcg agcgccacga cctggaccgc ggcccggcac
      421 agcttggtga cggcatgcgg cggcacggct ctggccgtgc tcatcggcgc gacggttgcg
      481 gtgctggtcg cactgaccga tatccgcggc cgcaacgcct ttgtcttctg cttcgtgctg
      541 ccgctgatga tcgcgccgca ggtcgtggca ctcgcgtggc tgcagttgtt cggaccgtcg
      601 agcacgctgc tcaagatgat cggcctcgct ccggcgatgg gcagccgcaa cccgctctac
      661 tccgcgcatg gcatcattct gctgctgggc gttcagtatg cgccgctggt gttcctcact
      721 ttgcgcgcgg gactgcgcac cttgccgcag gagctgctcg aagcggggct cgcggcgggc
      781 gcgaagccgc cgacgattct gcgcacgatc gtcctcccgc tgatgacgcc gccgctggtc
      841 gcaggcatcg cgctctgctt cgtctcgtgc cttggcaact tcggcattcc ggcgtttctc
      901 ggcattccgg gcaactacct ggttctgccg acgctgatct accagcgact ggccggcctg
      961 gggccgagcg tgctgtccga ggtcgccgtg ctgtcgtgcc tgataggctt catcgccgtg
     1021 ctgggcatct ttctgcagga cctgatgctg cgccggcgcg attttcgcat cacgacgaca
     1081 tcgaacgcga tgcgcccgtt cgagctcgca cgctggcgtc ccgccgtcga gatcgcactg
     1141 tggattttaa ccatcgtcgt tctggtattg ccgctggtcg cgctgctgtt gagctcgctg
     1201 gtgcctgcgg ccggcgtgcc gctgacggcg aagtccgcga cctggtcgaa ctacgattac
     1261 gtgctgttcg atcatgcggc cgcgaagcgc gctttggtca acagtttcag cctgtcggcg
     1321 acggccgcca tcgtcatcgt cctggtcgcc gttccgctcg cgtatttcct ggtctggcgc
     1381 cgctcgcggc ttttgcgcgt gctgaacctg gccgccgaga tgccatacgc gctgcccggc
     1441 gtcgtgctcg cgatcgccgc cattctggtc ttcctgaaac ccctgccggt gctcgggctc
     1501 acgctgtaca acaccgtctg gatcattctg ttcggctatc ttgcgcgctt tctcgtgctg
     1561 ggcctgcgtc cggtgatcgg cggctattac cagctcgatc gcacgctcga ggaagccgcc
     1621 cagatcgccg gcgcgcgttt gttgcgacgc ctggcgacga tcatcctgcc gctggtcgct
     1681 ccgatggcga ccgccggcgc gctgctggtg tttctcaccg cgttcaacga gctgacggta
     1741 tcggcgctgc tgtggtcgtc tggcgccgag acgcttggtg tcgtcgtgtt ttcgttccag
     1801 cagggcggcg actcgaccta tgccgcggcg ctggcgatgc tgaccgtcgc cgtgacgatc
     1861 gcgctgatgc tctcgacttt gctcaccggc aagcccgcaa ggggtgtgct gccatggcgc
     1921 gactgatcgt cgattgcgtc accaagacgt tcggcgcgct gcacgcgctc gaccaggtct
     1981 cgctcgacgt cggcgacggc gagttcatgg cgattctggg gccgtccggt tgcggaaaga
     2041 cgactttgct gcggcagatc gccggtttcg acaagctcga cggcggccgc atcgtcatcg
     2101 gcgaaacagt cgtctcgacc gtcacgcagc acgtgccgcc cgagcggcgc cgcatcggca
     2161 tcgtcttcca gtcgtacgct ctgtggccgc acatgacggt cgccgagaat gtcgcatatg
     2221 gcttgacggt cgccggcgtg cgcgacccgg aacgcacgcg gcgcgtcgac gccgcactga
     2281 cgctggtcga gctcgaaggc tacgccgagc gccgaccctc gatgctgtcg ggcgggcagc
     2341 gccagcgcgt ggcgctcgca cgctgcctgg tcaccgagcc ttcgctggtg ctgctcgacg
     2401 agccgctggc gaacctcgac gtgcatctgc gcgcttcgat ggaagaagaa ttcgcccgct
     2461 tccacgcgcg caccgggacg acgatggtgt acatcacgca cgaccaggcc gaagcgatgg
     2521 cgctggccga ccgcatcgcg gtgatggacc gtggcaggct gctgcaagtc gccacgccat
     2581 cgcaactcta tcgcgagccg gctgacgcgt ccgttgcggc atttatcggc gagggcatcg
     2641 tcgtacctgc ggaagtcatc gacgtcgaca gcagaggacg ctgccgtgtc gacctgttcg
     2701 gcttgagcgt cagcatgcgt tgcccgctcg cgcagacgcc gacgcgcgct gcgcgcgtct
     2761 gcctgcgcgc cgcgaacctg gccatcgtcg gcgccggtgt gcccggtatc ggcgctcgcg
     2821 tcgttcgctc cgtctaccag ggcggccact tccgggtcga agcgcagctc gaccatgccg
     2881 acggcgtgct cgtgcacctg gcggcggccg agccctgccg gctcgcaccc ggcgacgcaa
     2941 tacgcatcgg catcgacgac ggatgggtcc ttcctgaaac cggcgagatc gtcgattcga
     3001 ccgcgccgga gtcggctgat agccgtcgct tcgacggcgg cacagtcgcg accggcgcgg
     3061 acggctaaag gctcatgcgg ctgcatctgt atggaggctt cggcgaaaag ggccgcacct
     3121 gcctgggtgt cgaagtagac ggctatcgat tgctgctcga tgccggcgtg aagaccagcg
     3181 cacgcggcgc cgattattac ccggcgatca cgacggacga gctcggcgcg accgacgcga
     3241 tcgtcatcac gcatgcccac gaggaccacg tcgcggcatt gggctggtgc atagcgggtg
     3301 ggtttcgcgg ccgcctgctg atgaccgccg agaccggacg cgaagtcgat ctgtttctcg
     3361 aagcctatgc cgacgcctcg catcgcggcg ctgcgcgcga gttcgcgcgg gaggagctgc
     3421 cggtcggcgc ttcaaccgcg ctcgggccgc tgcaattgat cgccggccgc tctggacacg
     3481 tgtccggcgg cgtctggtgc accgtcgccg acggccgtcg caccctcggc tattgcggcg
     3541 acgtcgttcc ggcaagcgag gtattcgcga tggatccgct gccgcgctgc gacgcagtcg
     3601 tgctcgacgc ctcgtatgg
//
LOCUS       DATANS010000640         3156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_578004, whole genome shotgun sequence.
ACCESSION   DATANS010000640 DATANS010000000
VERSION     DATANS010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578004"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="VHT22_09405"
     CDS             complement(<1..1116)
                     /locus_tag="VHT22_09405"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX3137814.1"
                     /translation="MPPRYRRLLSFALALLAAPAIAQSILPAAVPPPSIAAKAYLLVD
                     LVSGQTLAADNADTPREPASLTKLMTAYLAFRALKDKELTPSQMVNVSEKAWRAEGSR
                     MFIEPKKAVSVDELLHGVIVQSGNDASIALAETVAGSEDAFAERMNREAARLGMKNTH
                     FVNATGLPAPKETSTANDLALLAAALIRDFPEYYPLYSIKEYRYNNITQPNRNRLLWT
                     DPYVDGMKTGNTEAAGYCLIASAKRGQRRLLAVVLGAGSESARAIEAQKLLNYGFQFF
                     DSVQLYQSGQPVSSLRVWKGAEQTVPAGFIVDRYVTLPKGQAQNLKLSLEAVEPLLAP
                     VVKGQTVGTVKVTLDGKPIGAYPLLALADVAPASLFGR"
     gene            1229..2131
                     /locus_tag="VHT22_09410"
     CDS             1229..2131
                     /locus_tag="VHT22_09410"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEX3137815.1"
                     /translation="MRSSIRLGLAAAILLFVAACATPLPPTTPRQPTDADRDSLAASE
                     LAFAKRSVEQGMRAAFIEYFADDGINFAPDPGNTRARYRARPEAIEPFILDWHPAIAA
                     VARAGDLGFTTGPYAITGRAPAVREPAQGMFFSVWRRQPDGDWRVIVDGGAPTRAPVP
                     DAAFGEDPQPLISIAKAAGAAGQKTLFELEAQPLFGEPAAANGYAALLADEARLVIGP
                     TPPALGRDAIVALLAERQLKFRWETLAAGVSRSDDLGYSYGRLHLLAAREKTTGHYVH
                     VWMRDRGGHWRIVAELMLLPEGSG"
     gene            complement(2128..>3156)
                     /locus_tag="VHT22_09415"
     CDS             complement(2128..>3156)
                     /locus_tag="VHT22_09415"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3137816.1"
                     /translation="LDWQQWRWGAQFLLECLPWRTRRNMLQILAISKYSGEMLRELRA
                     TTMLAYDDLQRGILQIYTDRADYDEAAAAAELVRSYGIARDVKTAAECVAIEPALETR
                     RDWIVGGTYTASDESGDARKFTQGLAARSFARGVVFRFGVTIDSIDVAGGAVSGVVAH
                     VATADGEPRRQLLTADAYVVCLSSYTPRLLAPVGIHVPVYPAKGYSATLPIVDMAAAP
                     SVSVTDDAKKIVFTRLGNRLRAAGTAELSGYNLDLNIVRCEALTRRAGEWFGGAIDTT
                     RAEYWTGLRPATPSNVPLIGRTRYPNLYLNTGHGTLGWTMAAGSGKAIADLVSGRKPD
                     IDFAFVGY"
BASE COUNT          526 a         1064 c         1039 g          527 t
ORIGIN      
        1 acgcccgaac aggctcgcag gcgccacgtc agcgagcgcc agcagcggat aagcaccgat
       61 cggcttgccg tcgagcgtga ccttgaccgt tcccaccgtc tgacccttga ccaccggcgc
      121 cagcagcggc tcgaccgctt ccagcgacag cttgagattc tgagcctgcc ccttcggcaa
      181 ggtcacatac cggtcgacga tgaacccggc gggcactgtc tgctcggcgc ccttccagac
      241 gcgcagcgag ctgaccggct gtccgctctg atagagctgc accgaatcga agaactggaa
      301 tccgtagttc aacagcttct gcgcttcgat cgcgcgcgcc gactccgacc cggccccgag
      361 gacgacggcc agcagccggc gctgtccgcg ctttgccgac gcgatcaggc aatagccggc
      421 ggcctcggtg ttgccggtct tcatgccgtc gacgtacgga tcggtccata gcaggcggtt
      481 gcgattcggc tgcgtaatgt tgttgtagcg gtactccttg atcgaataca gcgggtaata
      541 ctcgggaaaa tcgcggatca gtgcggcggc gagcagcgcg agatcgtttg ccgtggacgt
      601 ttccttcggc gccggcagac cggtcgcgtt gacgaagtgg gtattcttca tgccgagccg
      661 cgccgcctcg cgattcatcc gctcggcgaa cgcatcctcc gacccggcga cggtttcggc
      721 cagcgcgatg cttgcatcgt tgcccgattg gacgatcacg ccatgcagca gctcgtcgac
      781 gctcaccgcc tttttcggct cgatgaacat gcgcgagccc tcggcacgcc aggccttctc
      841 ggatacgttg accatctgcg aaggcgtgag ctccttgtcc ttgagcgcgc gaaacgcgag
      901 gtacgccgtc ataagcttgg tcagagacgc cggctcgcgc ggcgtgtcgg cgttgtcggc
      961 agcgagcgtc tgcccgctca ccaggtcgac cagcagatat gccttggccg cgatcgaagg
     1021 cggcggaacg gcagcgggaa gtatgctctg agcgatcgcc ggagcggcaa gcagggcgag
     1081 cgcgaaggac agcagtcgtc gatatcgggg aggcatggag aaaagcgtgc gtgcagtcgc
     1141 gcattataac ggaggccgca gacggactgg cgcgcggcag cgtcatgcgc tctaatccca
     1201 ctaacggcga agtgtggaaa ggcttgcgat gcgttcatcc atcaggttgg gacttgccgc
     1261 ggctattctg ctcttcgtcg ccgcctgcgc gacgcccctg ccgccgacga cgccgcgtca
     1321 gccgacggat gccgaccgcg actcgctggc ggcatccgag ctcgcgtttg ccaagcgctc
     1381 ggtcgagcag ggcatgcgcg ccgcgttcat cgaatacttt gccgacgacg gcatcaactt
     1441 cgcgcccgat ccgggcaaca cccgggcgcg ttaccgcgcc cgtcccgagg cgatcgagcc
     1501 gttcatcctc gactggcatc cagccatcgc cgcggtggcg cgtgccggcg atctcggatt
     1561 caccacaggc ccgtatgcga ttaccggccg cgctcccgcg gtgcgcgagc ccgcgcaggg
     1621 catgttcttt tcggtctggc gccgccagcc ggacggcgac tggcgcgtga tcgtcgacgg
     1681 cggcgcaccg acccgcgcgc cggtacccga tgcggcgttc ggggaggatc cgcagccgct
     1741 gatatcgatc gcgaaggccg ccggcgcggc cggtcaaaag accttgttcg agctggaagc
     1801 gcagccgctg ttcggcgagc cagccgcggc gaacggctac gcggcgctgc tggccgacga
     1861 ggccagactc gtcatcggtc cgaccccgcc agcgctgggc cgcgacgcga tcgtcgcgct
     1921 actggccgaa cggcagctca agtttcgatg ggaaacgctc gccgccggcg tatcgcgctc
     1981 cgacgatttg ggctacagct acggccgcct gcatcttctc gccgctcgcg aaaagacgac
     2041 gggccactac gtccacgtct ggatgcgcga ccgcggtgga cactggcgca tcgtcgccga
     2101 gctgatgctg ctgcccgagg gatcgggtta gtagccgacg aaggcgaagt cgatatcggg
     2161 cttgcggccc gaaaccaggt cggcgatcgc cttgcccgac cccgcggcca tcgtccagcc
     2221 aagcgtgccg tgacccgtat tcagatagag gttcggatag cgcgtcctgc cgatcaacgg
     2281 aacgttcgat ggcgtcgcag gcctcaagcc ggtccagtat tcggcgcgcg tcgtgtcgat
     2341 cgcgccgccg aaccattcgc cggcgcggcg cgtcagcgcc tcgcagcgca cgatattgag
     2401 gtcgaggttg tagccggaga gctccgcggt tccggcggcg cgcagccgat tgcccaggcg
     2461 cgtaaacacg atttttttgg catcgtcggt gacgctgacc gacggcgccg cggccatatc
     2521 gacgatcggc agtgtcgcgg agtagccctt ggccggatag acagggacgt ggatgcccac
     2581 cggcgccaaa agccgcggcg tatagctcga caggcagaca acgtaggcgt cggcggtcag
     2641 cagctgtcgg cgtggttcgc cgtcggcggt tgcaacgtgc gcaaccacgc cggaaacggc
     2701 gccgccggca acatcgatcg aatcgatcgt cacgccgaag cgaaagacga cgccgcgcgc
     2761 gaacgatcgc gcggcgagcc cctgcgtgaa cttgcgcgca tcgccgcttt cgtcgctcgc
     2821 ggtataggtc ccgccaacga tccagtcgcg cctggtctcg agcgccggct cgatcgcgac
     2881 gcattccgcc gccgtcttga cgtcccgcgc gatgccgtaa ctgcgcacca gctccgccgc
     2941 cgccgcggcc tcatcgtaat cggcgcggtc ggtgtagatc tgcaggatgc cgcgctgcag
     3001 gtcatcatag gcaagcatcg tcgttgcgcg aagctcgcgc aacatctcgc ccgaatactt
     3061 gctgatcgcg agtatctgca gcatgttgcg ccgcgtgcgc cagggcaggc attcgagcag
     3121 gaactgcgct ccccagcgcc actgctgcca gtcgag
//
LOCUS       DATANS010000641         3468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_284357, whole genome shotgun sequence.
ACCESSION   DATANS010000641 DATANS010000000
VERSION     DATANS010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284357"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VHT22_09420"
     CDS             complement(<1..205)
                     /locus_tag="VHT22_09420"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX3137817.1"
                     /translation="MDAIEALTTRASPLALTEPAPDAGALAAMLRAAARAPDHGKLKP
                     WRFIVIAGEARNAFGDVLAEALRK"
     gene            405..866
                     /locus_tag="VHT22_09425"
     CDS             405..866
                     /locus_tag="VHT22_09425"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX3137818.1"
                     /translation="MAMINVEVQATIRRPLAVVSRQFGDMRHHERHRVHPDIGLTVLS
                     EDGDDCRFRQEVRLLGMLQSDELVQHRNADGSLSSEVVAGANKGLRFHQGFASVGADS
                     TLVTFRAEAPATGIKRLLKPLFEIAIRRAVKKGLEEDRIDLEERGYGAATA"
     gene            947..1369
                     /locus_tag="VHT22_09430"
     CDS             947..1369
                     /locus_tag="VHT22_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX3137819.1"
                     /translation="MPSRSPTDLMWAQAFDLIEQAERIHRQFFRLSASARPHAVWEPP
                     VDVFEDEREVVIVVALPGVPPERIEVSTESTTLVVRAECPLPFAGSRRGVERLEIPYG
                     YFERRIPLPDARLEAGTRESSDGLLIVRLRKTAYTGPL"
     gene            1492..>3468
                     /gene="lon"
                     /locus_tag="VHT22_09435"
     CDS             1492..>3468
                     /gene="lon"
                     /locus_tag="VHT22_09435"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX3137820.1"
                     /translation="MIIVPVRNVVLFPGMVVPLVVDRERSRAAVQEAVRLQRPLGILL
                     QSRRDDDQPGPDDLHWVGTTATVLRYVTAPDGSHHVICQGVQRFRALQFLDGYPMLAA
                     QVQLIDEPAQTDVDIEGRALNLKERAIEILRLLPQVPEEMVAALQGVEGPARLADFIA
                     GLMDVSVEEKQALLETFDLKARLDKLLDLLTHRIEVLKVSREIDQRTKASIDDRNREH
                     LLREQMRTIQKELGDGDQTASEIAELDKAITEAKMPEEVEKQARKELKRLERMPEGAG
                     EYSMVRTYLDWLIELPWTADAEGTIDIAEARRILDEDHYGLEKIKKRILEYLAVRKLN
                     PSGKSPILCFVGPPGVGKTSLGQSIAKATARKFVRVSLGGVHDEAEIRGHRRTYIGSL
                     PGNIIQSLRKAGTRNCVLMLDEVDKLGQSMHGDPASALLEVLDPEQNSTFRDNYLGVP
                     FDLSGVMFICTANVLDTIPGPLRDRMEVIQLPGYTAQEKLQIARRYLVARQLAATGLK
                     PEQCEISDEALSAIIDDYTREAGVRNLEREIGRVFRSVAVRIAEGSAQQVKISPADLP
                     AILGPRIFEAEVAMRKPVPGVATGLAWTPVGGDILFIEAARMPGNGKLILTGQLGEVM
                     KESAQAALSLVKARAASLGIPTESLDSLDKADIHV"
BASE COUNT          636 a         1138 c         1164 g          530 t
ORIGIN      
        1 gcttacgcag ggcctcagcc agcacgtcgc cgaaagcgtt gcgtgcttcc ccggcgatga
       61 cgatgaagcg ccagggcttc aacttgccgt ggtccggcgc gcgcgcggcg gcgcgcagca
      121 tcgccgccag cgcgcccgcg tccggcgcag gctcggtcag ggcgagcggg ctggcccgcg
      181 tagtcaaggc ttcgatcgca tccaaaggcc agtggtcttt cagaggttcg tcatgcgggc
      241 agcgccgcgc gcgggtgcaa ggaaccgctt gttcgatcgc gcagcacgct cggttgcgag
      301 caggaaaatc gggacgtctg ttgtagcata accgatcggc ccggcaatcg cgatttcccg
      361 ccttcggata agcgcgatgc gtgtcacgcg aggagatcgt cacgatggct atgatcaacg
      421 tcgaggtaca agcaacgatc cgccgcccgc ttgcggtcgt cagccgccaa ttcggcgaca
      481 tgcggcacca cgagcgccac cgcgtccacc cggacatagg cttgaccgtg ctttcggagg
      541 acggcgacga ctgccgcttc cgccaggagg tgcggctgct cggcatgctg cagtccgatg
      601 agctcgtgca gcatcgtaat gccgacggaa gcctttcctc ggaagtcgtc gccggcgcca
      661 acaagggctt gcgcttccac cagggatttg cttcggtcgg cgccgactcg acgctcgtga
      721 cgttccgtgc cgaagcgccc gccaccggca tcaagcgctt gctgaagccg ctgttcgaga
      781 tcgccattcg cagggcggtc aaaaagggtc tggaggagga tcgcatcgac ctcgaggagc
      841 gggggtacgg cgctgcgaca gcctgaaagg cggcagtcgc gggttgccgg ggctgcgctt
      901 gaaccgggcg ccattgccca catctaaccg ggtacgagag accacgatgc catcgcgaag
      961 cccaaccgac ctgatgtggg cgcaggcctt tgacctgatc gagcaggccg agcgcatcca
     1021 ccggcaattc ttccgcctga gcgcatcggc gcggccgcat gcggtctggg agccgccggt
     1081 cgacgtgttc gaggacgagc gcgaggtggt catcgtcgtt gctctccccg gcgttccgcc
     1141 ggagcgaatc gaggtgagca ccgagtcgac aacactggtc gtacgcgccg agtgcccatt
     1201 gcctttcgcc ggatcgcgcc gtggggtgga acgcctcgag attccgtatg gctatttcga
     1261 gcgaaggatt ccactgcccg acgcccgact cgaggcgggc acgcgcgaat ccagcgacgg
     1321 cttgctgata gtccgattgc gcaagacagc ctatacggga ccactgtgaa cgaaccgacc
     1381 gaaaacgaac ccatcccggc cagcgccgac gcgaccgcgc gggccgacgc actcgcgcct
     1441 cagggcaagc gccagggcga ggctgtctcg cgcccgctac ccgacgacgc gctgatcatc
     1501 gtcccggtgc gcaatgtcgt cctgttcccc ggcatggtcg tgcccttggt cgtcgaccgc
     1561 gagcgttcgc gggcggcggt gcaggaagcc gtgcggctgc agcggccgct ggggattctt
     1621 ctgcagagca ggcgcgacga cgaccagccg ggacccgacg acctgcactg ggtaggcacc
     1681 accgcgacgg tattgcgcta cgtcaccgca cccgacgggt cgcaccatgt gatctgccag
     1741 ggcgtgcagc gctttcgtgc gctgcagttc ctcgacggct atccgatgct ggccgcacag
     1801 gtgcagctga tcgacgagcc cgcccagacc gatgtcgata tcgaaggccg cgcgctcaat
     1861 ctgaaggagc gcgcgatcga gatcctgcgc ctgttgcctc aggtgcccga ggaaatggtc
     1921 gcggcgctgc aaggcgtcga gggcccggcg cggctcgccg atttcatcgc cggactcatg
     1981 gacgtatccg tcgaggaaaa acaggcgctg ctggaaacgt tcgacttgaa ggcacggctc
     2041 gacaagctgc tcgaccttct gacccaccgc atcgaggtgc tgaaagtctc gcgcgaaatc
     2101 gaccagcgca ccaaggcatc gatcgatgac cggaaccgcg agcacctgct gcgcgagcag
     2161 atgcgcacga tccagaagga gctcggcgac ggcgaccaga cggcgagcga gatcgccgag
     2221 ctcgacaagg ccatcaccga agcgaaaatg cccgaggagg tcgaaaagca ggcgcgcaag
     2281 gaattgaagc gtcttgagcg gatgcccgaa ggcgccggcg agtactcgat ggtgcgcacc
     2341 tatctggact ggctgatcga gcttccctgg acggcggacg ccgagggcac gatcgacatc
     2401 gccgaggcgc ggcgcatcct cgacgaggat cactacggcc tggagaagat caagaagcgc
     2461 attctcgaat atctggcggt gcgcaagctc aacccgtcgg gcaagagtcc gatcctgtgc
     2521 ttcgtcggac cgccgggagt cggcaagacc tcgctcggcc agagcatcgc caaggcgacc
     2581 gcgcgcaagt tcgtgcgggt gagcctgggc ggcgtgcacg acgaggcgga gatccgcggc
     2641 catcggcgca cctacatcgg gtcgctgccg ggcaacatca tccagagcct gcgcaaggcg
     2701 ggcacgcgca actgcgtgct gatgctggac gaggtcgaca agctcgggca atcgatgcac
     2761 ggcgatcccg cgtcggcgct gctcgaagtg ctcgaccccg agcagaattc gacgttccgc
     2821 gacaactatc tcggcgtgcc gttcgatctg tccggcgtca tgttcatctg cacggcgaac
     2881 gtcctcgaca ccataccggg accgttgcgc gaccggatgg aagtcatcca gcttccgggc
     2941 tataccgcgc aggaaaagct gcagatcgcg cgccgctatc tggtggcgcg ccagctcgcg
     3001 gccaccggtc ttaagccgga gcaatgcgaa atcagcgacg aggcgctctc ggccatcatc
     3061 gacgactaca cgcgcgaggc gggcgtgcgc aacctcgaac gcgagatcgg cagggtgttc
     3121 cgcagcgtcg cggtgcgcat cgccgagggc agtgcgcagc aagtgaagat cagccccgcc
     3181 gatctgccgg caatcctcgg cccgcgcata ttcgaggccg aggtcgcgat gcgcaagccc
     3241 gtgcccggcg tcgcgaccgg cctggcgtgg acgccggtcg gcggcgacat cctgttcatc
     3301 gaggcggcgc gcatgcccgg caacggcaag ctgatcctga ccggccaact cggcgaagtg
     3361 atgaaggaaa gcgcgcaggc ggcgctgtcg ctggtcaagg cgcgcgctgc ttcgctcggc
     3421 atcccgaccg agagcctcga cagcctcgac aaggccgaca tccacgtc
//
LOCUS       DATANS010000642         1522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_158201, whole genome shotgun sequence.
ACCESSION   DATANS010000642 DATANS010000000
VERSION     DATANS010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158201"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1522)
                     /gene="hyfB"
                     /locus_tag="VHT22_09440"
     CDS             complement(<1..>1522)
                     /gene="hyfB"
                     /locus_tag="VHT22_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HEX3137821.1"
                     /translation="SLYAADYFAVSNYGNVRLICLQYHLFLASMAIVLLADDAYLFMV
                     AWETMALASFFLVTTDHVVPEIRRAGFLYLVIAHIGAVAILLCFGVLQGGSGDYTFGS
                     MRSLILPGAWPSIAFLLALAGFGAKAGLLPLHIWLPEAHPAAPSPVSALLSAVMLKTA
                     IYGLLRVTFDLLNTQLWWWGVTALVLGLLTALFGVIYATVQVDMKRLLAYSSIENIGI
                     IVAGIGLTILFKANGKTLLASIAMVATLYHALNHAFFKSLLFLATGSVLHSTRERSLG
                     KLGGLIHRMPWVAWSALIGALAIAGLPPLNGFVSEWLLLQAFLFTPTLPQSFINMLVP
                     LAAAALVLVAALGAYVMVKFYGVVFLGRPREPHLAYAHDAGDFERVALAWFATGCVVL
                     GLFPGTVIGLLDNVNMTLIGATVGHAGDRWWLLAPISPDRSSYSPLIVLLVIAAVVAL
                     TIQVVHRWYHGRVRSGPAWDCGFPMQTPRMQDTAEGFGQPIRQVFEMFFRVERVLPT"
BASE COUNT          328 a          481 c          503 g          210 t
ORIGIN      
        1 gtcggcagca ctcgctcgac gcggaagaac atttcgaata cctgccggat cggctggccg
       61 aagccttccg ccgtgtcctg catgcgcggc gtctgcatcg gaaagccgca atcccacgcc
      121 gggccgctgc ggacgcgccc gtggtaccag cgatgaacaa cctgaatcgt cagcgcgaca
      181 acagcggcga tcaccagcag cacgatcagt gggctatagc tcgagcggtc tgggctgatc
      241 ggcgcgagga gccaccatcg gtcgccggcg tggccgaccg ttgcgccgat cagcgtcatg
      301 ttgacgttgt cgaggagccc gatgacggtc ccgggaaaca atccgagcac gacgcagccg
      361 gtcgcgaacc acgccagggc gacgcgctcg aaatctccgg cgtcgtgcgc gtacgccagg
      421 tgaggttccc gcggacgtcc gagaaagacg acgccgtaga acttgaccat cacgtacgcg
      481 ccgagtgcgg cgaccagcac gagcgccgct gccgcgagcg gcaccagcat gttgatgaaa
      541 gattgcggca gcgtcggcgt gaacaggaaa gcctgcaaca gcagccactc ggaaacaaag
      601 ccgttgagcg gcggcagccc ggcgatcgcc agcgcgccga tcagcgccga ccatgcgacc
      661 cagggcatgc gatgaatcag ccccccgagc ttgccgaggc tgcgctcgcg cgtggagtgc
      721 agcaccgaac cggtggcgag aaacaggagg ctcttgaaga acgcgtgatt gagcgcgtgg
      781 tagagcgtcg ccaccatggc gatcgacgct agcagcgtct tgccgttcgc cttgaacagt
      841 atcgtcaggc cgatgccggc gacgatgatg ccgatgttct cgatcgacga gtacgcgagc
      901 agacgcttca tgtcgacctg cacggtggcg tagatgacgc cgaacagcgc ggtcagcaag
      961 ccgagcacca gcgccgtgac accccaccac cacagctggg tgttgagcag gtcgaaagtc
     1021 acccgaagga gaccgtagat tgcggtcttc agcatcactg ccgagagcag cgcggatacc
     1081 ggcgagggtg ctgccggatg cgcttcgggc agccagatgt gcagcggcag cagcccggcc
     1141 ttggcgccga atccggccag ggccagcaga aacgcgatcg acggccacgc gccaggcagg
     1201 atcagcgagc gcatcgagcc gaacgtgtag tcgccgctgc cgccctgcag tacgccgaag
     1261 cagagcagga tcgctaccgc gccgatgtgc gcgatcacca ggtaaaggaa gcctgcacga
     1321 cggatctcgg gaacgacgtg atcggtcgtc acgaggaaga acgaggccag cgccatcgtc
     1381 tcccacgcca ccatgaacag gtaagcatcg tcggcgagca gcacgatcgc catgctcgcg
     1441 aggaacaagt gatattgcag gcagatcaag cgtacgttgc cgtaattgct caccgcgaag
     1501 tagtcggcgg catagagcga ta
//
LOCUS       DATANS010000643         1526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_200785, whole genome shotgun sequence.
ACCESSION   DATANS010000643 DATANS010000000
VERSION     DATANS010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200785"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VHT22_09445"
     CDS             complement(<1..363)
                     /locus_tag="VHT22_09445"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX3137822.1"
                     /translation="MIPKSPPQGRSVDEYRAPLFIAWQLTNRCAGRCIACCEASGPGN
                     AWPDELDRGEALDLARRIAQSGIPYVAFGGGEPLSVAHCWELFDRLTAAGISLKIETD
                     GTRIDDAAADRLAALAVQC"
     gene            complement(360..503)
                     /locus_tag="VHT22_09450"
     CDS             complement(360..503)
                     /locus_tag="VHT22_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137823.1"
                     /translation="MSESKTTVDVASADAVTMAPPPKLVWETPKIEDVSEQVMAQPYI
                     RFT"
     gene            complement(520..>1526)
                     /locus_tag="VHT22_09455"
     CDS             complement(520..>1526)
                     /locus_tag="VHT22_09455"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX3137824.1"
                     /translation="AVRRRHRTARVANRIMTYAIDSLRAPLLVTWQLTRDCDLCCLHC
                     CTDSAPGKRLRDELDADEAMRVVRGMVDVGVPYVMLCGGEPLVVPHFMDVAEALGSAG
                     IQLKIETNGQRLDARLVERLARLPIRSIQVSLDGDTQAVYERQRPGASLAKAHAACRA
                     IRDAQLPLEITFAPSKLNLHEASAVIERARSLGAFRFNTGRLMRVGTAARTWGKLAPS
                     EEQYRAFRAELALQERVLEPAMQFCYLPFSIEDGLRAALEQPPATLLVLPNGWVKVAA
                     AIPEICADLRRETFVDAWRAYREAWRCGPVRAAIRAAIEDESLHGRANAWQVLQVIQV
                     "
BASE COUNT          257 a          520 c          493 g          256 t
ORIGIN      
        1 gcactgcacc gcaagcgccg cgagccgatc tgccgctgcg tcgtcgatgc gcgtaccgtc
       61 ggtctcgatc ttgagggaga tgcccgccgc agtcagccgg tcgaagagtt cccagcagtg
      121 agcgactgag agcggctcac cgccgccgaa ggctacgtag ggaatgcccg attgtgcgat
      181 gcgccgggcg aggtccaagg cctccccgcg atcgagctcg tcgggccacg cattgcccgg
      241 cccggacgct tcacagcatg caatgcagcg cccggcgcag cgattcgtga gctgccaggc
      301 gatgaacagg ggcgcccggt actcgtcgac cgagcggccc tgcggcggtg atttcggaat
      361 caggtgaagc ggatatacgg ctgcgccatt acctgctcgg agacgtcctc gatcttgggc
      421 gtttcccaca ccagcttcgg cggcggcgcc atagttaccg cgtcggcgct cgctacatca
      481 acggtcgtct tggattcgct catgctgcct cctctacgtt catacctgga ttacttgcaa
      541 cacctgccac gcattcgccc ggccatgaag cgattcgtcc tcgatcgcgg cccggatcgc
      601 ggccctgacc gggccgcatc gccaggcctc acgatacgct cgccaggcat cgacgaacgt
      661 ttcgcggcgc agatcggcgc agatctcggg aatggccgca gccactttca cccaaccgtt
      721 cggcagcacc agcagagtgg cgggcggctg ttccagcgcg gcgcgcaatc cgtcctcgat
      781 actgaaggga agatagcaga actgcatggc gggttcaaga actcgctcct gcagcgcgag
      841 ctcggcgcga aaagcccggt actgctcttc gcttggggcg agtttgcccc aggtacgcgc
      901 ggcggtgccc acgcgcatca gcctgccggt gttgaaacga aacgcaccca gcgatcgcgc
      961 ccgctcgatg acggcgctcg cctcgtgcag gttgagcttt gacggggcaa acgtgatctc
     1021 cagcggcagc tgcgcgtcgc ggatcgcgcg gcatgccgcg tgcgctttgg cgagcgatgc
     1081 cccgggccgc tgccgctcgt agaccgcctg cgtgtcgccg tcgagactta cctgaatcga
     1141 tcggataggc agccgagcca ggcgctcgac caagcgcgcg tcgagacgct ggccgttcgt
     1201 ctcgatcttt agctggatgc ccgcgcttcc cagtgcttca gccacatcca tgaagtgcgg
     1261 cactaccagc ggctcgccgc cgcacagcat cacgtacggg acgccgacat ccaccatgcc
     1321 gcggacgacg cgcatcgcct cgtccgcgtc gagctcgtca cgcaaccgtt tgcccggagc
     1381 ggagtcggtg cagcagtgca ggcagcagag gtcgcagtcg cgcgtgagct gccaggtcac
     1441 gagcagcggt gcgcgcaggc tgtcgatcgc gtatgtcatg atgcgattcg ccacgcgtgc
     1501 agtacgatgc cggcggcgaa cagccc
//
LOCUS       DATANS010000644         3301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_578059, whole genome shotgun sequence.
ACCESSION   DATANS010000644 DATANS010000000
VERSION     DATANS010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578059"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1038
                     /gene="thiL"
                     /locus_tag="VHT22_09460"
     CDS             70..1038
                     /gene="thiL"
                     /locus_tag="VHT22_09460"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX3137825.1"
                     /translation="MTEFELIERYFRRPARNAVLGVGDDAALIAPSPGFEIAASVDML
                     VEGRHFFPGTEAEALGHKTLAVNLSDLAAMGATPRWALLACALPSVDEAWLAAFARGF
                     FALADEHAVDLVGGDTTRGPRDLCVTVLGEVPAGKALRRSGASGGDSIWVSGKLGDAA
                     LAIAHQLGRIRLEGADLAACENALARPAPRVTLGLALRGIASAAIDVSDGLVGDLGHV
                     LSASRIGATIDLDALPRSAALERQLGGADRAIAQQCLLAGGDDYELCFTAPRARDARI
                     AAIGDKLGLPLTRIGIATAQRAGLEVRDASGRALEPLPRAFDHFAP"
     gene            1056..1541
                     /locus_tag="VHT22_09465"
     CDS             1056..1541
                     /locus_tag="VHT22_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEX3137826.1"
                     /translation="MKKTPTLRFMLAHPAHLVALGFGTGLSPIAPGTVGTLLALPLDA
                     LLRATVNDVGYAIAVVVAFAIGVWAADRTGRDLGVSDHGAIVWDEVVAFLGVLYFVGA
                     EPVYQAFAFLLFRLFDIVKPPPAGLIDREWHRGIGVMADDLVAAGYTLLAIALWQRTF
                     G"
     gene            1538..2311
                     /locus_tag="VHT22_09470"
     CDS             1538..2311
                     /locus_tag="VHT22_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3137827.1"
                     /translation="MSGRSGRRAEARRLEEMALNASGAFQSLLYDGWLLGHRAGPTKR
                     LRCINPTYRSSLPLEQKLAYCTDFYRTAGLPAIFRMLPFAQPVGLDAFLAERGWSEFE
                     RTLVLRAGLAAAIALPAPAVEVELVAMMAWVEATRALLGIASEALPRLLDRAANYPLP
                     QIGALVRRDDEVVACGLAKVEEDHVGLFAVHTAPSLRGQGLGRAVVGALLADAQRRGA
                     RTAYLQVTAQNAAALALYRRFGFDTAYDYWYRARQGEQH"
     gene            2308..2811
                     /locus_tag="VHT22_09475"
     CDS             2308..2811
                     /locus_tag="VHT22_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX3137828.1"
                     /translation="MKATSPPNDDDIQALAARLGELALRRQVMLATAESCTGGLVAGA
                     ITDIAGSSGWFERGFVTYSNRAKIEQLGVDPEIIARHGAVSEETVKAMAQGAMAASGA
                     QWTVAVTGVAGPAGGSPAKPVGTVWFAWAGPQGVEALPRRLDGDRAAVRAASVAIALQ
                     GLIDRLV"
     gene            2942..>3301
                     /locus_tag="VHT22_09480"
     CDS             2942..>3301
                     /locus_tag="VHT22_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX3137829.1"
                     /translation="MDDNKSKALSAALAQIEKQFGKGSIMKMGEAQVQNDLSVVSTGS
                     LGLDIALGVGGLPRGRVVEIYGPESSGKTTLCLQVVAQVQKAGGTAAYIDAENALDPV
                     YAGKLGVNVGEMLISQPD"
BASE COUNT          486 a         1069 c         1184 g          562 t
ORIGIN      
        1 cgacgggcac aaatacgtca atggcgtgct cgacaagctg gcggcgactg cgcgcgcggc
       61 ggaagccggt tgaccgaatt cgagctcatc gagcggtatt tccggcgccc ggcgcgcaac
      121 gccgtgctcg gcgtcggcga cgacgcggcg ctgatcgcgc catcgcccgg tttcgaaatc
      181 gccgcgtcgg tcgacatgct cgtcgagggc cggcattttt tcccgggcac ggaagccgag
      241 gcactcggcc acaagacgct ggcagtcaac ctgtcggatc tggcggcgat gggtgcgacg
      301 ccgcgctggg cgctgttggc gtgtgcgctg ccctcggtcg acgaagcctg gctggcggcg
      361 ttcgcgcgcg gattttttgc gctggcggat gagcatgccg tcgatctcgt cggcggcgac
      421 acgacgcgcg gtccgcgcga tctttgcgtc acggtgctcg gcgaagttcc cgccgggaaa
      481 gcgttgcgtc gcagcggagc gagcggcggc gattcgatct gggtctccgg caagctcggc
      541 gatgcggcgc tggcgatcgc gcaccagctg ggcaggatcc ggctcgaagg cgcggatctt
      601 gcagcctgcg agaacgcgct ggcgcgtcct gccccgcgcg ttacgctggg cctcgcgctg
      661 cgcggcatcg ccagcgccgc gatcgatgtg tccgacggcc tggtcggcga tctgggccac
      721 gtgctcagcg cttccaggat cggcgccacg atcgatctcg atgcgctgcc gcgctcggcc
      781 gcgctcgagc gacagctcgg cggcgcggat cgcgcgatcg cgcagcaatg cctgctggcg
      841 ggcggcgacg actacgagct ctgcttcacg gcgccgcggg cgcgcgatgc ccgcatcgcg
      901 gccatcggtg acaagctcgg cctgccgctg acgcgaatag gcatcgctac ggcgcaacgt
      961 gcagggttgg aggtccgcga tgcgagcggg cgcgcactcg agccgctgcc gcgcgcgttc
     1021 gaccatttcg cgccgtgact ggggccagtg cgtcgatgaa aaaaacgccg accttgcgtt
     1081 tcatgctcgc gcatccggcg cacctggtgg cgctcggttt cgggaccggg ctctcgccga
     1141 tcgctccggg caccgtcggc acgctgctcg cgctgccgct cgacgcgctg ctgcgcgcga
     1201 cggtcaacga cgtcggttac gcgatcgccg tcgtcgtcgc cttcgcgatc ggcgtctggg
     1261 ctgccgaccg caccgggcgc gatctggggg tatccgatca cggcgcgatc gtctgggacg
     1321 aggtcgtcgc cttcctcggc gttctctact tcgtcggcgc cgagccggtg taccaggcct
     1381 ttgcgttcct gctgttccgg ctgttcgaca tcgtcaagcc gccgccggcg gggttgatcg
     1441 accgcgaatg gcatcgcgga atcggtgtga tggccgacga cctggtcgcc gccgggtaca
     1501 cgctgctggc gatcgcgctg tggcaacgga cattcggatg agcggccgtt ccgggcggcg
     1561 cgctgaggca cgccggctcg aggaaatggc gctcaatgcc tccggcgcct tccagtcgct
     1621 gctttacgac gggtggcttc tcggtcaccg cgccggtccg accaagcgtc tgcgttgcat
     1681 caacccgact tatcgatcgt cgcttccgct cgagcaaaag cttgcgtact gcaccgactt
     1741 ctatcggacc gccggcttgc cggcgatctt tcgcatgctg ccgttcgcgc agcccgtggg
     1801 gctcgatgcg ttcctcgccg agcgtggctg gagcgaattc gagcgcacgc tggtcctgcg
     1861 cgccggtctt gccgcggcta tagccttgcc cgcgccggcg gtggaggtcg agctcgtcgc
     1921 gatgatggca tgggtcgagg ccacgcgagc actgctcggc atcgcaagcg aagcgctgcc
     1981 gcggctgctc gaccgcgcgg ccaactatcc gttgccgcag atcggtgcgt tggtgcgccg
     2041 cgacgacgag gtcgtcgcct gcgggctggc gaaagtcgaa gaggatcacg tcggattgtt
     2101 cgccgtgcac accgcccctt cgttgcgcgg gcaaggcttg ggacgcgcag tcgtcggggc
     2161 gctgctggcg gatgcgcagc ggcgcggcgc ccggaccgcc tacttgcagg tcacggcgca
     2221 aaatgctgcc gcgctcgcgc tctacaggcg gttcggcttc gacaccgcgt acgactactg
     2281 gtaccgggcg cgacagggcg aacagcattg aaagcgacat cgccgccgaa cgacgatgac
     2341 atccaggcgc tggccgcgcg cctgggcgag ctggcgctgc gccgtcaggt aatgctggcg
     2401 acagcggaat cgtgcaccgg tggattggtc gccggggcga tcaccgacat cgccgggagt
     2461 tcgggctggt tcgagcgggg ctttgtcacc tattcgaacc gtgccaagat tgagcagctg
     2521 ggtgtcgatc ccgagatcat cgcgcgccat ggcgcggtca gcgaagagac cgtaaaggcg
     2581 atggcacagg gggcgatggc cgcgagcggg gcgcaatgga cggttgcggt gacgggggtc
     2641 gccggcccgg cggggggcag tcccgccaag ccggtaggaa ccgtctggtt tgcctgggcc
     2701 gggccgcagg gcgtcgaggc gctgccccgc cggttggacg gggaccgggc agccgtacgc
     2761 gccgcctcgg tggcgatcgc cttgcagggc ctaatcgacc ggctggttta ggccataaga
     2821 aagctttatc gaacgatcgt tctatgccat aatggtctca tccttcgccg cttggcggag
     2881 cgccaccaga gagaccgcga tgagcaacgt aagcctggtt aaatccaatg gaatgaacgc
     2941 catggacgac aacaagagca aggcgcttag cgccgcgctg gcccagatcg aaaagcagtt
     3001 cggcaagggc tcgatcatga aaatgggcga ggcccaggtc cagaacgacc tgtcggtggt
     3061 ctcgaccggg tcgcttggcc tcgacatcgc gctgggcgtc ggcggtttgc cgcgcgggcg
     3121 ggtcgtcgag atctatgggc ccgagtcatc gggcaagacc accttgtgcc tgcaggtcgt
     3181 cgcgcaggtg cagaaggccg gcggcaccgc tgcctacatc gacgcggaaa acgcgctcga
     3241 tccggtctac gcggggaaac tcggcgtcaa tgtcggcgag atgcttattt cgcagcccga
     3301 t
//
LOCUS       DATANS010000645         1651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_617498, whole genome shotgun sequence.
ACCESSION   DATANS010000645 DATANS010000000
VERSION     DATANS010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617498"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1185
                     /gene="metC"
                     /locus_tag="VHT22_09485"
     CDS             49..1185
                     /gene="metC"
                     /locus_tag="VHT22_09485"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEX3137830.1"
                     /translation="MHLGREPARHLGAINTPVYRASTILFESVADYEASQRGQSKSLN
                     YGLRGLPTVTDLQAAVATLEGGHAALAVPSGLAATTFPLLALTSAGDHVLVTDSVYGP
                     TRRFCNNHLTRLGIEVSYYDPLIGSGIEREMRPNTRAVFLESPGSLSFEVQDVSAIAA
                     VAHAHGALVLLDNTWATPLNFRAFDHGVDVSLHAGTKYLGGHSDLLIGLIVGNKASFP
                     RLHRLWTDTGVTASSDDCFLALRGLRTLAVRLERHQASALRIAQWLRDRDEVAEVVYP
                     ALPGARGHELWKRDFTGACGLFGVILKPAPKARVDAMLDGLSWFKLGVSWGGFESLIL
                     PINDAVRSATRWAPEGPYIRLHVGLEDPDDLIDDLAGGLARLRA"
     gene            1307..>1651
                     /locus_tag="VHT22_09490"
     CDS             1307..>1651
                     /locus_tag="VHT22_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137831.1"
                     /translation="MSNLLPLHPPLATLDDDAWDDLLNFIEERRVIPIIGPELLKVET
                     ETGPRLLYDWIAEKLAGKLNVDTTRLPQPYTLNDVVCWFLSSHGRREEAYTRVRSILR
                     DANFTPPLALRQL"
BASE COUNT          257 a          563 c          548 g          283 t
ORIGIN      
        1 actgatcagc gcaagcccgg ccgcaagccc ggagcggcga ccgaagtcgt gcatctcggg
       61 cgcgagcccg cacggcatct gggcgcgatc aacacgccgg tctatcgcgc atcgacgatt
      121 ctgttcgagt cggtcgccga ctacgaagcg tcgcagcgcg ggcagtcgaa gagcctgaac
      181 tacggccttc gcggcctgcc gacagtcacc gacctgcagg cggcggttgc gacgctcgaa
      241 ggcggccatg ccgcgctggc cgtgccttcg ggtctggccg cgacgacctt ccctttgctc
      301 gcgctgacca gcgccggtga tcacgtgctg gtcaccgatt cggtctacgg cccgacgcga
      361 cgcttctgca acaaccatct gacgcgattg ggcatcgagg tcagctacta cgatccgctg
      421 atcggtagcg gcatcgagcg cgagatgcga cccaacacgc gcgctgtgtt cctcgaatcc
      481 cccggctcgc tgagcttcga ggtccaggat gtttcggcga tcgccgccgt ggcgcacgcg
      541 cacggcgcgc tggtgctgct ggacaatacc tgggcgacgc cgctcaattt tcgcgccttc
      601 gaccatggcg tcgacgtttc gctgcatgcg ggcaccaagt acctgggcgg acattccgac
      661 ctgctgatcg gcctgatcgt cggcaacaag gcgagctttc cgcgcctgca ccggctgtgg
      721 accgacaccg gcgtcaccgc gagcagcgac gactgctttc tggcgctgcg gggactgcgg
      781 acgctggcgg tgcggctgga gcgccaccag gccagcgcgc tgcgcatagc gcagtggctg
      841 cgcgaccggg acgaggtcgc cgaagtcgtc tacccggccc tgccgggcgc gcgagggcac
      901 gagctgtgga agcgtgattt caccggcgcc tgcggactgt tcggcgtgat cctgaagccc
      961 gcgccgaagg cgcgggtcga cgcgatgctg gatgggctct cctggttcaa gctgggagtc
     1021 agctggggcg gcttcgagag tctgatcctc ccgatcaacg acgccgtgcg cagcgcgacg
     1081 cgctgggcgc cagaaggtcc gtacatccga ctgcatgtcg gcctcgagga tccggacgat
     1141 ctgatcgacg accttgccgg cggattggcg cggctgcgcg cctgacagtt cgcttgcggt
     1201 gcgcaggcca ggcccggcgg actgcccggt cgcgatgatc gtttgaccct gctttcggaa
     1261 tcgtggacac tctgccggcc cgctatcctt ggccctcgac tcccggatga gcaatctgct
     1321 tccgctgcac ccgcctctgg ccacgctcga tgacgatgcg tgggacgatc tgctcaactt
     1381 catcgaggag cggcgcgtca ttccgatcat cgggccggag ctgttgaaag tcgagaccga
     1441 aaccggtccg cgcctgctct acgactggat cgccgaaaag ctcgccggca agctgaacgt
     1501 cgatacgacg cgactgccgc aaccctacac gctcaacgac gtcgtctgct ggttcctgtc
     1561 gtcccacggg cggcgcgagg aggcctatac gcgcgtgcgc agcatcctcc gcgacgcgaa
     1621 tttcacgccg ccgctggcgc tgcgccagct g
//
LOCUS       DATANS010000646         1524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_827161, whole genome shotgun sequence.
ACCESSION   DATANS010000646 DATANS010000000
VERSION     DATANS010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827161"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..1431)
                     /locus_tag="VHT22_09495"
     CDS             complement(256..1431)
                     /locus_tag="VHT22_09495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006513657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3137832.1"
                     /translation="MAVVAQSVLNEPLLGRCRERAPEYDRNNQFFQEDFDELKSAGYL
                     RMALPKEFGGLGMNIAEVGRETRTLAMYAPATALALNMHNYWVGDAADAWRSGDKSLQ
                     WILEEAAAGEVFAAGHAEHGNDIPGLLSTTKAERVDGGYKFTGRKSFGSLTPVWTRLG
                     LHGMDMSDPAAPKIVHAFMPRDSKGYSIKDTWDVLGMRATRSDDTILEGAFIPDKYIV
                     RRVPAGAAGADNFVVGFFTWALVGFGNIYYGLALRVREVLIEQLKTKTSIALTRPMIY
                     HPEVQHGIAEISMDIEAMGPHVEAVATAWAEGNVGPDWLVRLMSMKHKVVEGAFRVAD
                     RALDLSGGFGMFKKSELERLFRDARAGKFHPANPLLTHEIVGKVSLGINPDEQPRWG"
BASE COUNT          263 a          502 c          461 g          298 t
ORIGIN      
        1 atggcggtga tgcgtccgtt ggtgacgacg atggtcgcct gctcgatcgg cggagcgcct
       61 gtgccgtcaa tgacccgcgc cccaacgaac gccacgactc ccggcgccgg aagctgcgcg
      121 aacgccggcg cggcgaccgc gaccaacacg aaggtcgcga tcgcggcggc gcatttccga
      181 ctcacccact tcccgcttgg atacgtcaca cttatttttt cacttttcaa ctttttcact
      241 ttcccacttt cctggttagc cccaccgtgg ctgctcgtcg ggattgatgc cgagcgatac
      301 cttacccaca atctcgtgcg tcagcaatgg attcgcgggg tggaatttgc cggcacgcgc
      361 gtcacgaaac agccgttcga gctcgctttt cttgaacatg ccgaacccgc cggagagatc
      421 gagggcgcga tcggcgacgc ggaacgctcc ttcgaccacc ttgtgtttca tcgacatgag
      481 ccgcacgagc caatcgggac ccacgttccc ttcggcccac gccgttgcga cggcctcgac
      541 gtgcggaccc atcgcctcga tgtccatcga gatttccgca ataccgtgct gcacctcggg
      601 gtggtagatc atcggccgcg tcagcgcaat cgacgttttc gtcttcaact gctcgatgag
      661 cacctcgcgc acgcgcagcg ccaggccgta gtagatgttg ccgaagccga cgagtgccca
      721 ggtgaagaaa ccgacaacga agttatcggc acccgctgcg ccggcgggaa ccctgcgcac
      781 gatgtacttg tcggggatga acgcgccctc gagaatggtg tcgtcgctgc gagtcgcgcg
      841 catgccgagc acatcccacg tgtccttgat cgaatagccc ttgctgtccc gcggcatgaa
      901 ggcgtgcacg atcttgggcg ccgccgggtc gctcatgtcc atgccgtgaa gaccgaggcg
      961 cgtccacacc ggtgtcaggc tgccgaatga tttgcggccg gtgaacttgt agccgccatc
     1021 gacgcgttcc gctttggtcg tggacagcag gccgggaatg tcgttgccgt gctccgcgtg
     1081 cccggcggcg aacacctcgc cggccgccgc ttcctcgagg atccactgca gcgacttgtc
     1141 gccgctgcgc cacgcgtcgg cggcatcgcc cacccagtag ttgtgcatgt tcagcgcgag
     1201 cgcggtcgcc ggcgcgtaca tcgccagggt gcgcgtttcg cggccgactt cggcgatgtt
     1261 catgccgagg ccgccgaatt ccttcggcag cgccatgcgc agatatcccg cgcttttcag
     1321 ttcgtcgaaa tcttcctgga agaattgatt gttcctgtcg tactcgggcg cgcgttcgcg
     1381 acacctcccg agcagcggct cgttaaggac cgactgggca acgacggcca tggctatacc
     1441 tccggtgatg agagcgagga cagatatgct gcgcgtgagc gcgaacgcgg gagtatatac
     1501 cgctattcgt gcgacaaaac gacc
//
LOCUS       DATANS010000647         3413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_200891, whole genome shotgun sequence.
ACCESSION   DATANS010000647 DATANS010000000
VERSION     DATANS010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200891"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="VHT22_09500"
     CDS             <1..1181
                     /locus_tag="VHT22_09500"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3137833.1"
                     /translation="AQQLEVARRWSRAMLPPPAPRRRPQPRANRLRVGYLSGSFHEHP
                     TARLMVGLFEAHDRERFEVTGYSYGPDDGSPLRARVRASFEHWRDVRELSDQDIARAI
                     REDGIDLLIERKGHTHGGRLAILAQRPAPVQIHYMSFPGTIGYDAVDGVIADAEVVPP
                     GADVDFHERVWRLPRCYYVNDNRRGVPRASPRRDNGLPEDALVLACLNQGYKLRRPFF
                     SVWMEALRAQADAVLWLLAGHPRAQANLRAEAAKSGVDPARLIFARAAPQDAHVARVA
                     CADLALDTLPYGAHTTGVDALWAGVPMLTCRGDTFAGRVGASLLLAAGLPELVANTLD
                     DYRARLLSLSADRAALHGYRAYLEGSRDSNPLFDTKAFARDWEALLLTIYSHAAGIEA
                     "
     gene            complement(1188..1997)
                     /locus_tag="VHT22_09505"
     CDS             complement(1188..1997)
                     /locus_tag="VHT22_09505"
                     /inference="COORDINATES: protein motif:HMM:NF025089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3137834.1"
                     /translation="MTGAGRGPTLPRFSLIVCSVDRARLDECVARYRAAFADEAFDLI
                     VIDDAKSLAEAYNRGIARARGANLVFSHDDAFPISIAFADRLAAHLEQVDVVGIAGAT
                     AALSGFWGYAGQPHTHGHVIASAPQGSHVDLLVWGASRRRVDDIRLLDGCMIAARRDV
                     AVAIGFDAQRFDGFHLYDADFSFRASAAGHAVAVASDITVFHRSPGSFDAEWHRYNEK
                     FVAAHSASFDRIAPQPRLVARVAFADIAAAAKDVDERRVAELTLQLRAASA"
     gene            complement(1994..2374)
                     /gene="gloA"
                     /locus_tag="VHT22_09510"
     CDS             complement(1994..2374)
                     /gene="gloA"
                     /locus_tag="VHT22_09510"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEX3137835.1"
                     /translation="MRILHTMLRVGDLERSVAFYTGVLGMNVLRTTDRPEQKYTLAFV
                     GYGDESAHAVLELTYNYGVDRYALGDGFGHIAIEVPDVAAACAEVRAKGGTVTREAGP
                     VKGGTTVIAFVQDPDGYKIELIQR"
     gene            complement(2390..2554)
                     /locus_tag="VHT22_09515"
     CDS             complement(2390..2554)
                     /locus_tag="VHT22_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015389172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX3137836.1"
                     /translation="MKKYMCLICGHIYDEAYGDPDTGIPPGTKWEDVPPNWTCPDCGA
                     RKEDYELVEF"
     gene            2665..>3413
                     /gene="thiD"
                     /locus_tag="VHT22_09520"
     CDS             2665..>3413
                     /gene="thiD"
                     /locus_tag="VHT22_09520"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX3137837.1"
                     /translation="MPQPSDAESDFPPVVLTFAASDPSGGAGIQADILTLASMGCHPL
                     SVITAITVQDTLGVEAVQPIDADWVADQARCLLEDMPVDAFKIGALGSVENIAAIAEI
                     LSDYPDVPLILDPVLASGRGDELANDEMLHALRELLLPRTTILTPNSMEARRLADIED
                     EEEPSLATCANRLIESGCEFVLITGTHEPTPQVVNTLYGKSGVVRSDSWQRLPGNYHG
                     SGCTLASAIAAMLANGLDLAEAVREAQDYTW"
BASE COUNT          584 a         1122 c         1145 g          562 t
ORIGIN      
        1 ctgcccagca gctcgaggtc gcgcgccgct ggtcgcgcgc gatgcttccg ccgcccgcgc
       61 cgcggcggcg gccgcagccg cgggccaacc gtttgcgcgt aggctacttg tccggcagtt
      121 ttcacgaaca tccgaccgcg cgtctgatgg tcggcctttt cgaggcgcat gaccgcgaac
      181 gcttcgaggt gaccgggtac agctacgggc cggacgacgg gagtccgttg cgcgcgcgcg
      241 tgcgcgcctc gttcgagcat tggcgtgacg ttcgggagtt gtcggaccag gacatcgcgc
      301 gggcgatccg cgaagacgga atcgatctgt tgatcgagcg caaaggccac acccacggcg
      361 gcaggctggc catcctcgcg cagcgacccg ccccggtgca gatccactac atgagctttc
      421 ccgggacgat aggctatgac gcggtcgacg gcgtcatcgc cgacgccgag gtcgtgccac
      481 cgggtgcgga cgtcgatttt cacgagcgcg tatggcgact tccgcgatgc tattacgtca
      541 acgacaaccg gcgcggcgta cctcgcgcca gcccgcgacg cgacaacggt ctgccggaag
      601 acgcgctggt gctcgcgtgc ctcaaccagg gctacaaact ccgccggccg ttcttctccg
      661 tgtggatgga ggcgttgcgc gcccaagccg acgccgtgtt atggctgctc gcggggcacc
      721 cgcgagcgca ggccaatctg cgcgccgaag ccgcgaaaag cggcgtcgat ccggcgcggc
      781 tgatcttcgc gcgcgccgcg ccgcaggacg cgcatgtcgc gcgggttgcc tgcgcggacc
      841 tggcgctcga cacgctgcct tacggcgcgc acacgaccgg cgtcgatgca ctgtgggcgg
      901 gcgtgccgat gctgacctgc cgcggcgaca cgttcgcggg cagggtcggt gcgagcctgc
      961 tgctcgcggc cggcttgccc gagctggtgg cgaatactct cgacgactac cgcgcgcgcc
     1021 tattgtcgct ttcagcagac cgcgcggcat tgcatggcta ccgcgcctac ctcgaaggca
     1081 gccgcgacag caacccactg ttcgacacaa aagccttcgc ccgcgattgg gaagcgctgc
     1141 tgctgacgat ctacagccac gcggcgggga tcgaagctta agcctgccta agcgctggca
     1201 gcgcgcaact gcagcgtcaa ctccgccaca cgccgctcat cgacatcttt cgccgcggct
     1261 gcgatatcgg caaatgcgac tcgggcgacc agccgcggct gtggagcgat tcggtcgaag
     1321 ctcgccgaat gcgctgcgac gaacttctcg ttatagcggt gccattcggc gtcgaacgat
     1381 ccaggagagc gatgaaagac ggtgatgtcc gaggccaccg ccaccgcatg tccggcggcg
     1441 ctggcgcgga acgaaaaatc ggcgtcgtac aagtggaagc cgtcgaaacg ctgcgcgtcg
     1501 aagccgattg cgacagccac atcgcggcgc gccgcgatca tgcatccgtc gagcagcctg
     1561 atgtcgtcga cccggcggcg ggacgcgccc cagaccagca gatcgacatg cgatccctgg
     1621 ggcgcggagg caatgacgtg gccatgggtg tgcggctggc ccgcgtagcc ccagaagccg
     1681 gatagcgctg cggtcgcgcc ggcgatgccg acgacgtcga cctgttcgag atgcgcggcc
     1741 agccggtcgg cgaatgcgat ggagatcgga aacgcgtcgt cgtgcgagaa caccaggttg
     1801 gcgccgcgcg cgcgggcgat accgcggtta taggcttcgg cgagagactt cgcgtcgtcg
     1861 atcacgatca ggtcgaacgc ttcgtcggcg aaggcagcgc ggtaacgcgc gacgcactcg
     1921 tcgaggcgcg cgcgatcgac gctgcagacg atgagggaga agcgcggcaa ggtcgggccc
     1981 cggccggcgc cggtcaacgc tggatcagct cgatcttgta accgtccgga tcctgaacga
     2041 aggcgatgac cgtggtgccg cccttgaccg gaccggcctc gcgcgtgacg gtgccgcctt
     2101 tggcgcggac ctccgcgcat gcagcggcga cgtccggcac ctcgattgcg atgtggccga
     2161 aaccgtcacc gagcgcgtag cggtcgacgc cgtagttgta agtgagctcc agcaccgcat
     2221 gcgcggattc atcgccgtag ccgacgaatg cgagcgtgta tttctgttcg gggcggtcgg
     2281 tcgtgcgcag gacgttcatt ccgagcacgc cggtataaaa ggcgaccgat cgctcgagat
     2341 cgccaacgcg caacatcgtg tgcaggattc gcatcgtttt ggcaggacgc tagaattcga
     2401 ccagttcgta atcttccttg cgggcgccgc aatcgggaca tgtccagttg ggcgggacgt
     2461 cttcccattt ggtgcccggc ggtatgccgg tgtcgggatc cccgtaggcc tcatcataga
     2521 tatgtccgca gatcaggcac atgtattttt tcattcgcta cggctcgact ggagtaaaat
     2581 cccattttag caagcattcg ccggccttta gcgtcggccc gggcatccca gcgtgcgccc
     2641 ggttgcactg aaagcgccgc cgcaatgccg cagccgtccg acgccgagtc cgatttcccg
     2701 cccgtagtgc tgactttcgc cgcatccgat ccgtcgggcg gcgccggcat ccaagccgac
     2761 atcctgacgc tagccagcat gggttgccat ccgctgtcgg taatcaccgc gatcaccgtt
     2821 caggacacgc taggcgtcga agcggtgcag ccgatcgacg ccgactgggt cgccgatcag
     2881 gcacgctgcc tgctcgagga catgccggtc gatgctttca agatcggcgc gctcggcagc
     2941 gtcgagaata tcgccgcgat cgccgaaatc ctgtctgatt acccggacgt cccgttgatt
     3001 ctcgacccgg tgctggcttc gggacgcggc gacgagctgg cgaacgacga aatgctgcat
     3061 gcgctgcgcg agctgctgct gccgcgcacg acgatactga cgccgaacag catggaagcg
     3121 cgtcggctcg ccgacatcga ggacgaggag gagccgtcgc tcgctacctg cgccaatcga
     3181 ctgatcgaaa gcggctgcga gttcgtgctg atcaccggaa cgcacgagcc gaccccgcaa
     3241 gtggtcaaca cgctgtacgg caagagcggt gtcgtgcgca gcgacagctg gcagcggctg
     3301 cccggcaatt atcacggctc gggttgcacg ctggcctccg cgatcgcggc gatgctggcc
     3361 aacgggctcg acctggcgga ggcggtgcgc gaagctcagg actacacctg gca
//
LOCUS       DATANS010000648         1579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_327652, whole genome shotgun sequence.
ACCESSION   DATANS010000648 DATANS010000000
VERSION     DATANS010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327652"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VHT22_09525"
     CDS             <1..186
                     /locus_tag="VHT22_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEX3137838.1"
                     /translation="LGGLLICLVVAGAFVGALMAVDLSKVVAVFFILAMLAIVACLGM
                     FLREVFLAVTGGTHRIP"
     gene            complement(205..786)
                     /locus_tag="VHT22_09530"
     CDS             complement(205..786)
                     /locus_tag="VHT22_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137839.1"
                     /translation="MIHGAASLANRALAREDWARDRLAAHAGRKLRIVAGPAALTLAI
                     NADGSFVDSDETPDLTLTIPALRLPTLIARPERWNEVVVATGDASLASTIGELAQTLP
                     WFIERSFARLFGPIVGPQLAEAGRRLLALPGYAAERAGSSFASYARDESGLVAGARAG
                     SEFAAEVAALAARVDALGERIDRLVKPAPAKRR"
     gene            complement(796..1530)
                     /gene="ubiE"
                     /locus_tag="VHT22_09535"
     CDS             complement(796..1530)
                     /gene="ubiE"
                     /locus_tag="VHT22_09535"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEX3137840.1"
                     /translation="MSEQTNFGYEQVTPEEKTRRVRGVFDSVATRYDLMNDLMSLGMH
                     RAWKRFTIAASGVRPGARVLDVAGGTADLARLFAARVGPTGSVVLTDINGAMLAAGRD
                     RMLNEGRLMPAVQCNAEALPFPAASFDCVSIAFGLRNVTRKDVALAEMRRVLRPGGVA
                     LVLEFSRVWAPLQPAYDWYSFNVLPRLGQVVANDAASYRYLAESIRVHPDQQELGAMM
                     ESAGLERVEWFNLSAGVVALHRGYVF"
BASE COUNT          258 a          532 c          531 g          258 t
ORIGIN      
        1 ctgggcggct tgctgatctg cctggtcgtt gccggcgcgt tcgtcggcgc gctgatggcc
       61 gtcgatttat cgaaggtcgt ggcggttttc tttatcctcg cgatgctcgc catcgtcgca
      121 tgcctcggca tgttcctgcg cgaagtgttc ctcgcggtga ctggagggac gcaccgcatc
      181 ccctgaggcg ggcgggttgc gcgatcagcg gcgttttgcc ggagcaggct tgaccagacg
      241 gtcgattcgc tcgccgagag cgtcgacccg cgccgccaga gcagcgacct cggcggcaaa
      301 ttcactcccg gcgcgcgcgc cggcgacgag tccggattcg tcgcgggcat aactggcgaa
      361 gctcgacccg gcccgttccg ccgcatagcc gggcaatgcc agcaatcggc ggccggcttc
      421 ggcaagctgt gggcccacga tcggtccgaa taggcgagca aacgagcgct cgatgaacca
      481 cggcagcgtt tgcgccagct cgccgatcgt cgatgcgaga ctcgcgtcgc ccgtcgcgac
      541 gaccacctcg ttccatcgct cgggtcgcgc aataagcgtc ggcaagcgca gtgccggaat
      601 agtcagcgtc aggtcgggcg tctcgtcgct gtcgacgaag ctgccgtcgg cgttgatcgc
      661 cagagtcagc gccgccggtc cggcgacgat gcgaagcttg cggccggcgt gcgcggcgag
      721 ccgatcccgc gcccagtcct cgcgcgcaag cgcacgattg gcgaggctgg cggcgccatg
      781 gatcatgagg atcggctaga aaacgtagcc gcgatgcagg gcgacaacgc ccgcactcag
      841 gttgaaccat tcgacgcgtt cgagccccgc gctttccatc atcgcgccga gctcctgctg
      901 atccgggtgc acgcggatcg actcggcgag atagcgatag ctggccgcgt cgttcgcgac
      961 gacctggccg aggcgcggca gcacgttgaa gctgtaccag tcgtaagcgg gttgaagcgg
     1021 cgcccagacg cggctgaact cgaggaccag cgcgacaccg cccgggcgca gcacgcggcg
     1081 catctcggcg agcgctacat ccttgcgcgt gacattgcgc agaccgaacg cgatgctgac
     1141 gcagtcgaag ctcgccgctg gaaaaggaag agcctcggca ttgcattgca ccgcgggcat
     1201 cagccggcct tcgttcaaca tgcggtcacg ccccgcggcg agcatcgcgc cgttgatatc
     1261 ggtcagcacg acgctacccg tcggaccgac gcgcgcggcg aacaggcgag cgagatcggc
     1321 ggtgccgccg gcgacatcga gcacccttgc gccggggcga acgcccgaag ctgcgatcgt
     1381 aaagcgcttc caggcgcgat gcatgcccaa ggacatcagg tcgttcatca ggtcgtaccg
     1441 ggtcgccacc gaatcgaata cgccccgcac gcgcctggtc ttttcctcgg gggtcacctg
     1501 ctcgtagccg aagttcgtct gctcgctcat cgcttcagtg tccgtgaccg caggagtgcg
     1561 ctgtcgcccc cggcagcga
//
LOCUS       DATANS010000649         1638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_662379, whole genome shotgun sequence.
ACCESSION   DATANS010000649 DATANS010000000
VERSION     DATANS010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662379"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VHT22_09540"
     CDS             complement(<1..278)
                     /locus_tag="VHT22_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3137841.1"
                     /translation="MFDQVDFSALELPPSARALQREVREFLRTELAALGFTPHLGHAE
                     FHAGFTRRVAERGWIGMTWPKRYGGQERSYLDRFVVTEEMLAVAAPCAA"
     gene            complement(289..>1638)
                     /locus_tag="VHT22_09545"
     CDS             complement(289..>1638)
                     /locus_tag="VHT22_09545"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX3137842.1"
                     /translation="AAAESHTASMTADYELYRATFREGGFIEVRDVHEVVDLTRVFLG
                     RRMPRGNRIGIITTSGGSAVLMTDECDRHGLTLPRFADATVKAIEAIAPKHCALGNPV
                     DLTAQISGNDKDFNRIVELALNDPNMDQMIVRYGTVQGARAAAWAQDIAAIVEKADKP
                     LIVSLGRVAESATAATEVLDARRIPWVLTPTRTAVAAGALGQYAQKQRGYLAARARGA
                     RRVIAAQKLAFASDAPRLSEAESKQALVAYGIPIAQERVLAPAAIAALIEVPLKFPLV
                     VKVDSPDLPHKTEAGAVRLNVQTLAELKEAAAAVVANAMRYKPDAHINGVLVAEMATG
                     TEVIIGAVNDKFFGPVVMFGLGGVFAELLKDVTYRFAPFDVDTAREMVAEIKAAKLLT
                     GYRGSAPLAIEALADVLARVSLLAADHAEHIAEIDINPLFVNKTGVVAADALIVRKS"
BASE COUNT          280 a          528 c          537 g          293 t
ORIGIN      
        1 gccgcgcacg gcgcggcgac cgcgagcatt tcctcagtca cgacgaagcg gtcgagatac
       61 gagcgctcct gcccgccgta gcgtttgggc caggtcatgc cgatccagcc gcgttcagcg
      121 acccggcgcg tgaagcccgc atggaattcg gcatggccca gatgcggcgt gaagccgagc
      181 gcggcgagct cggtgcgcaa aaattcgcgc acctcgcgct gcagtgcgcg tgccgaaggc
      241 ggcaattcga gcgcggagaa gtcgacttga tcgaacattt gattgccgtc agcttttgcg
      301 gacgatcagc gcatcggcgg cgacaacgcc ggtcttgttc acgaacaatg gattgatatc
      361 gatctcggcg atgtgttcgg cgtgatcggc ggcgagcagc gagacgcgcg ccaacacgtc
      421 cgcgagcgcc tcgatcgcaa gcggtgcgct gccgcgatat ccggtgagca gttttgccgc
      481 cttgatctcc gcgaccatct cgcgcgcggt atcgacgtcg aacggcgcga agcggtaagt
      541 cacgtctttc aggagctcgg caaacacgcc gccgagtccg aacatcacga ccgggccgaa
      601 aaatttatca ttcacggcgc cgatgatgac ttcggtcccg gtggccatct cggcgaccag
      661 cacgccgttg atgtgcgcgt ccggcttgta gcgcatggca ttggcgacga cggccgcagc
      721 cgcttccttc aattcagcga gcgtttgaac gttgagacgc acggcgcccg cttcagtttt
      781 atgcggaagg tcgggcgaat cgactttaac cacgagcggg aatttgagcg gcacctcgat
      841 aagcgcggcg attgccgccg gcgccagcac gcgttcctgc gcaatcggaa tgccgtaggc
      901 aaccagtgcc tgcttggatt ccgcttcgct caggcgcggc gcgtcggacg cgaacgcgag
      961 tttttgcgca gcgatcacgc ggcgcgcgcc gcgcgctcgc gcggcgaggt aaccgcgctg
     1021 cttctgcgcg tactgaccga gcgcaccggc ggccaccgcg gttcgcgtcg gcgtcagcac
     1081 ccatggaatg cgccgcgcat cgagcacttc ggttgccgcg gtggcggact ccgcgacccg
     1141 cccgagcgac acgatcaacg gcttgtcggc tttctcgaca atcgcagcga tgtcctgcgc
     1201 ccatgccgcg gcacgcgcgc cttgcaccgt accgtagcgc acgatcatct ggtccatgtt
     1261 gggatcgttc aacgcgagtt cgacgatgcg gttgaagtcc ttgtcgttgc cgctgatctg
     1321 cgcggtgaga tcaaccgggt tgcccaatgc acagtgcttt ggggctatcg cttcgatggc
     1381 cttgacggtg gcatcagcga agcgcggcaa ggtcaacccg tgacggtcgc attcgtcggt
     1441 catcagcacc gccgagccgc cggacgtcgt gatgatgccg atgcggttgc cgcgcggcat
     1501 gcggcggccc aggaacacgc gcgtgaggtc gacgacttcg tgcacgtcgc gcacctcgat
     1561 gaagccgccc tcgcgaaacg tcgcgcggta aagctcgtaa tccgccgtca tgctcgcggt
     1621 atgcgattcg gcggccgc
//
LOCUS       DATANS010000650         2448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_239610, whole genome shotgun sequence.
ACCESSION   DATANS010000650 DATANS010000000
VERSION     DATANS010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239610"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1099
                     /gene="mnmA"
                     /locus_tag="VHT22_09550"
     CDS             2..1099
                     /gene="mnmA"
                     /locus_tag="VHT22_09550"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX3137843.1"
                     /translation="MGLSGGVDSSVAAWLLKRAGHEVVGVFMKNWEEDDSDGYCTSRQ
                     DLVDAASVADVIGIELEAVNFAAEYQDRVFAQFLHEYRAGRTPNPDVLCNSEIKFKAF
                     IDHARALGAERIATGHYARLERSEHGVELRKAADTGKDQTYFLHQLSQAQLDGTMFPV
                     GDLAKRDVRSIAREQGIPTYAKKDSTGICFIGERPFREFLARYLPTEPGPIETLSGET
                     VGRHQGLAYYTLGQRQGLGVGGTRGGGPAPWFVAGKDLARNALIVVQGHDDPRLYADV
                     VETATMHWITGAAPSGRRFAAKTRYRMADARCTLEPLAAGRWRVRFDAPQWAPTPGQY
                     LVLYDGETCLGGAVIESARACEVAPALAGAG"
     gene            1314..>2448
                     /locus_tag="VHT22_09555"
     CDS             1314..>2448
                     /locus_tag="VHT22_09555"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HEX3137844.1"
                     /translation="MFKRIREVSMLQCGPGLRAGERALCEQRAADLRGHAHPALDRLF
                     AFALVLLLALLMPAHSQAQSSGVPPDLEAQSAEYLPQDQLDALLAPIALYPDDLLAQV
                     LMASTYPLEVVEAARFVQQNPSLSGAALDDAVRTRRWDPSVQSLTAFPQVLAMMSDRL
                     EWTQQLGDAVLANRQQVMDTVQALRVRAEATGTLQDTEQQHVYTSDQAIVIEPAQFDY
                     VYVPVYDPLVAYGPWWVASYQPYFWYPPAYFGYPVHWHGVGIVWGRPCRVDRNHWGWA
                     RPAWRTGRIDVTIGRNEFFNRPQYRNYRGSVSWQHAPEHRRGVAYGSAQVHARFINAE
                     RRVIDARELRAGVPSAAPGRQPTAPQTTPGYRQAPSDARATPQT"
BASE COUNT          403 a          789 c          837 g          419 t
ORIGIN      
        1 ggtgggattg tcaggcgggg tcgactcgtc ggtcgcggcg tggctgctca agcgcgcagg
       61 ccacgaggtg gtcggcgtgt tcatgaagaa ctgggaggag gacgacagcg acggctactg
      121 cacgtctcgc caggatctag tcgacgccgc atcggtcgcc gatgtgatcg gcatcgagct
      181 cgaagcggtc aacttcgctg ccgaatacca ggaccgcgtg ttcgcgcaat tcctgcacga
      241 ataccgcgcc ggacgcacgc ccaaccccga tgtgctgtgc aacagtgaaa tcaagttcaa
      301 ggcctttatc gatcacgcca gggcgctggg cgccgagcgc atagccaccg ggcactatgc
      361 gcggctcgag cgcagcgagc acggcgtcga gctgcgcaaa gcggccgaca caggcaagga
      421 tcagacttat ttcctgcacc agttgtcgca ggcgcagctg gacgggacga tgtttccggt
      481 cggcgatctt gccaagcgcg acgtgcgctc gatcgctcgc gagcagggca taccgacgta
      541 cgccaagaag gattcgaccg gtatctgctt catcggcgag cggccgttcc gcgagtttct
      601 ggcgcgttat ctgccgaccg agcccggacc gatcgagacg ttgtcgggcg aaaccgtcgg
      661 ccggcatcag ggtcttgcct actacacgct cgggcagcgg caggggctcg gtgtcggcgg
      721 cacgcgcggc ggaggacccg caccgtggtt tgtcgccggc aaggaccttg cgcgcaacgc
      781 gctgatcgtc gtccagggcc acgatgatcc gcggctttac gccgacgtcg tcgagaccgc
      841 aacgatgcac tggattaccg gcgcggcgcc gagcggccgg cgtttcgctg ccaagacgcg
      901 ctaccggatg gcggacgcac gctgcacgct ggagccgctc gccgcaggcc gctggcgcgt
      961 gcgcttcgac gctccgcaat gggcgccgac gccggggcaa tatctggtgc tctacgatgg
     1021 cgagacctgc ctgggcgggg cggtgatcga atcggcgcgt gcgtgcgagg tcgcgcccgc
     1081 gctcgctgga gcgggctgac cgtcaccaaa gcgccgcgcg aacgcggttt ccgcgcctgc
     1141 gcccctcgcg gggtgcttgt cgtcggcgca acaagttgtt accaagaaaa tcgtccgcac
     1201 gttatctcct tggttcgccg agcgttaatg atcgggcaac gcgccgatac tcgatcggcg
     1261 ccgcgctgcc gcaggaacag gcacgcgatc ccgagtgtta gccggagcta gcgatgttca
     1321 aacgtattcg tgaggtgtcg atgttgcaat gcggtccggg tctccgtgcc ggcgagaggg
     1381 cgctgtgcga gcagcgcgct gccgacttgc gtggccacgc gcatcccgcg ctcgatcgtc
     1441 tgttcgcgtt cgcgctggtg cttttgcttg cgctgctgat gccggcgcac tcgcaggcgc
     1501 aatcgtcggg cgtgccgcct gacctcgaag cgcaatcagc cgaatacctt ccgcaggatc
     1561 agctcgatgc gctgctggcg ccgattgcgc tgtatcccga cgacttgctg gcgcaggtgc
     1621 tgatggcatc gacgtacccg ctggaagtgg tcgaagcggc gcgcttcgtc cagcagaatc
     1681 cgtcgctctc gggcgccgcg ctcgacgatg cggttcgaac caggcgctgg gatccgagcg
     1741 tgcaatcgct gacggcgttt ccgcaggtgc tggcgatgat gagcgatcgg ctcgaatgga
     1801 cgcagcagct cggcgacgcc gttcttgcca accggcaaca ggtgatggac accgtgcagg
     1861 cgctgcgcgt ccgcgccgaa gcgaccggca cgctgcagga caccgagcag cagcatgtgt
     1921 acacatcgga ccaggcgatc gtcatcgagc cggcgcaatt cgattacgtc tacgtgccgg
     1981 tctacgaccc gttggtcgcc tacgggccgt ggtgggtggc gtcctatcag ccgtatttct
     2041 ggtatccgcc ggcatacttc ggctacccgg tgcactggca cggcgtgggg atcgtctggg
     2101 gcagaccgtg ccgggtcgat cgaaaccatt ggggctgggc tcgtcctgca tggcggacgg
     2161 gccggatcga cgtgacgatc ggccgcaacg aattcttcaa ccgcccgcaa taccgcaact
     2221 accgcggcag cgtttcgtgg cagcacgcgc ccgagcaccg gcgtggcgtc gcttatggaa
     2281 gtgcacaggt gcacgctcgc ttcatcaacg ccgaacggcg cgttatcgac gcgcgcgaat
     2341 tacgagccgg cgtgccatcc gctgcgcctg gccgacaacc gaccgcgccg cagacgactc
     2401 cggggtatcg acaggcgcct tccgatgctc gcgcgacgcc gcagacgg
//
LOCUS       DATANS010000651         3920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_239634, whole genome shotgun sequence.
ACCESSION   DATANS010000651 DATANS010000000
VERSION     DATANS010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239634"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..603)
                     /locus_tag="VHT22_09560"
     CDS             complement(67..603)
                     /locus_tag="VHT22_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3137845.1"
                     /translation="MKRAPPIWLETLRLELREFTPEDIGDLVRLDSDPRVLKYINGGK
                     PMPKSEIDAAMRRVTRYYALYSGLGVWRASLRSSGKFIGWYCLKYCPPTTDIEVGYRL
                     LHAHWGKGYATEGARALVDYAFDDLGVTRVIGVTHPGNKASQHVLQKAGLSDLGWGNY
                     YDRRLRLFACERPADGRD"
     gene            complement(600..1703)
                     /gene="prfA"
                     /locus_tag="VHT22_09565"
     CDS             complement(600..1703)
                     /gene="prfA"
                     /locus_tag="VHT22_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX3137846.1"
                     /translation="MKPSLSTKLNQLTSRLEELNGLLAAEDVTRDLDRYRALSREHAE
                     IAPVVALYRDWLQGERDFAAAQDMLSDPEMKSFGDDEAKAARVRMVTLEDELQRALLP
                     RDVNDERNVFLEIRAGTGGDESALFAGQLFRMYARFAERNKWQVELISESPGDMGGYK
                     EVIARVVGQGVYAKLKFESGGHRVQRVPETEAQGRIHTSACTVAVLPEADPVADITIN
                     PSEIRIDTFRASGAGGQHVNKTDSAVRITHLPTGIVVECQDDRSQHRNRAQAMAVLAS
                     RLVDRERQERQQKEAATRKSLIGSGDRSERIRTYNFPQGRVTDHRINLTLYKIDQIMD
                     GDLFELTQALAQEFQAEQLAALGGDEGAGETVR"
     gene            complement(1815..3068)
                     /gene="hemA"
                     /locus_tag="VHT22_09570"
     CDS             complement(1815..3068)
                     /gene="hemA"
                     /locus_tag="VHT22_09570"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX3137847.1"
                     /translation="MPLYALGLNHATAPVKVREQVAFQPDTLGVALRDLIAQPSVKEA
                     AILSTCNRTEVYFSAAEAAPVERWLESFHRVPSESLHPYVYTLAQDRAVSHAFRVASG
                     LDSMVLGEPQILGQMKHAVRSAEAAGALGLILNRLFQRTFAVAKDVRTNTDIGSASIS
                     MAAAAVKLAERIYPSLADQHLLLIGAGEMIDLAAAHFAAKHPKSITVANRTLERGEQL
                     AARFGANAITLNELPERLAQFDMIVTSTASSLPILGKGLLERAIKSRRHTPMFIVDLA
                     VPRDVEPEAAELDDVFLYSVDDLANIVKDNLQIRVDALTQAEEMIAEQAQSFLRWLDG
                     RSVIPTLTALQGHHEVLRAAEVERARRLLSGGAAPEQVIEQLARGLTNKFLHAPAQAL
                     TRAGDAERAQLLAMLHHIYQLPDDD"
     gene            complement(3136..>3920)
                     /locus_tag="VHT22_09575"
     CDS             complement(3136..>3920)
                     /locus_tag="VHT22_09575"
                     /inference="COORDINATES: protein motif:HMM:NF025089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3137848.1"
                     /translation="DARLPAIGPVTPDRAVSIVICSIDDVRHERAVALYRRLFAAVRH
                     EIVSIRNARSLAEAYNWALRHSVADVVVLSHDDVDILASDFAVRLFERLQAFDVVGVA
                     GSTRSDGPAIGWSGHPHLRGWITHRVPDSANWQVDVLDPRPVAGDIVLLDGVLLAGKR
                     DAFQAVPFDDVNFDGFHLADLDWSYRAAKAGARLGVVGDLLLVHASRGNYDATWQRYA
                     DRFCRKHDAGRSPPAPSSFFGASLDNPEQVRAFFGYLAELSS"
BASE COUNT          662 a         1278 c         1306 g          674 t
ORIGIN      
        1 ggccagccat agcggccgct gacttgcctt ccggctgcgt cagtctacgc acctcgccgc
       61 ccacggctag tctcggccgt ccgcggggcg ctcgcacgcg aacaggcgaa gccgccggtc
      121 atagtaattg ccccagccca ggtcgctcag gcccgctttc tgcagcacgt gctgcgacgc
      181 cttgttgccg ggatgcgtga cgccgatgac gcgcgtgacg ccgaggtcgt cgaacgcata
      241 gtcgaccagt gcgcgcgcac cttcggtcgc atagcccttt ccccagtgcg cgtgcagcag
      301 gcgatagccg acttcgatgt cggtcgtcgg cggacagtac ttcaggcagt accagccgat
      361 gaacttgccg ctgctgcgca gcgatgcgcg ccataccccg agtcccgaat aaagcgcgta
      421 gtagcgcgtg accctccgca ttgccgcgtc gatctcgctc ttcggcatcg gcttgccgcc
      481 gttgatgtat ttcagcacgc gggggtcgct gtcgagccgc acgaggtcgc cgatgtcttc
      541 cggcgtgaac tcgcgcaact cgagtcgcaa cgtctcgagc caaatcggcg gcgcgcgctt
      601 catcgcacgg tttcgccggc gccttcatcg ccgccaaggg cggcgagctg ctcggcctgg
      661 aattcctgcg ccagcgcctg cgtcagctcg aacaggtcgc cgtccatgat ttggtcgatc
      721 ttgtagagcg tcaggttgat gcgatggtcg gttacccgcc cctgtggaaa gttgtaggtg
      781 cgaatgcgct cggagcgatc gccgctgccg atcaggctct tgcgcgtcgc cgcctccttc
      841 tgctgccgct cctggcgttc acggtcgacc agccgcgagg ccagcaccgc catcgcctgc
      901 gcgcggttgc gatgctgcga gcgatcatcc tggcattcga cgacgatacc ggttggcaaa
      961 tgggtgatgc gcacggccga gtcggtcttg ttcacgtgct ggccgccggc gccggatgcg
     1021 cggaacgtat cgatccggat ctcggatgga ttgatcgtga tgtcggcgac cggatcggct
     1081 tcgggcagca ccgcgaccgt gcacgccgac gtatggatgc gcccctgcgc ctcggtctcg
     1141 ggcacgcgct ggacccgatg gccgccggat tcgaatttga gcttggcgta cacgccctgc
     1201 cccacaacgc gagcgatcac ctccttgtag ccacccatgt cgccgggact ctcggaaatc
     1261 agctcgacct gccatttgtt gcgctccgca aagcgcgcgt acatgcgaaa gagctgaccg
     1321 gcgaacaatg ccgactcgtc gccgccggtg ccggcgcgaa tttcgagaaa tacgttgcgc
     1381 tcgtcgttga catcgcgcgg cagcagcgcg cgctgcagct cgtcctcgag cgtgaccatg
     1441 cgcacccgcg ccgccttcgc ctcgtcgtcg ccgaaagact tcatctccgg atcggacagc
     1501 atgtcctgcg ccgccgcgaa gtcccgctcc ccctgcagcc agtcgcggta gagcgcgacg
     1561 acaggcgcga tttccgcgtg ctcacgcgac agcgcgcggt agcggtcgag atcgcgagtg
     1621 acgtcctcgg ccgcgagcaa gccgttcaat tcctccagcc gcgacgtcag ctggttgagc
     1681 ttcgtggaga gcgacggttt catgtcgtgc gaacggtggc cttctcgcgg cggccggagc
     1741 gccgcattgc tgatcgggta gacggtcgta aagtcagcgt ccggcgtgcg ccggacggcg
     1801 agccggatcc gaagctaatc gtcgtccggc aactggtaga tgtgatgcag catcgccagc
     1861 agctgggcgc gctcggcgtc accggcgcgc gtcagcgctt gcgcgggcgc gtgcaggaac
     1921 ttgttggtga gcccgcgggc gagctgttcg atcacctgtt cgggcgcagc gccgccggaa
     1981 agcagcctcc gtgcgcgctc gacctcggcc gcgcgcagga cttcgtgatg gccctgcagc
     2041 gcggtcagcg tcggaatgac gctgcgtcca tcgagccagc gcaaaaaact ctgggcctgc
     2101 tcagcgatca tctcctcggc ctgcgtcagc gcatcgacgc ggatctgcag gttgtccttg
     2161 acgatgttgg ccaggtcgtc gacgctgtag agaaaaacgt cgtcgagctc ggcggcttcg
     2221 ggctcgacgt cgcgcggcac cgccagatcg acgatgaaca tcggcgtatg acggcgcgat
     2281 ttgatcgccc gctcgagcag acccttgccc aggataggca atgagctcgc ggtcgaggtc
     2341 acgatcatgt cgaattgggc caggcgctcg ggcagctcgt tcagcgtgat cgcattcgct
     2401 ccgaagcgcg cggccagctg ctcgccgcgc tcgagcgtgc ggttggcgac ggtgatcgac
     2461 ttcggatgct ttgcggcaaa gtgcgcggcg gcgagatcga tcatctcgcc ggcgccgatc
     2521 agcagcaggt gctggtcggc cagcgacgga tagatgcgtt cggcgagctt taccgccgcc
     2581 gcggccatcg aaatcgacgc gctgccgatg tccgtgttcg ttcgcacgtc cttggccacc
     2641 gcgaacgtgc gctgaaaaag gcgattgagg atcaggccca gagcgcccgc cgcctcggcg
     2701 ctgcgcaccg cgtgcttcat ctggccgaga atctgcggct cgccgagtac catcgaatcg
     2761 agcccgctgg cgacgcggaa cgcatgcgag accgcgcgat cctgcgccag tgtgtagaca
     2821 tagggatgta gcgattcgga gggcacccgg tggaagctct ccagccagcg ctcgaccggc
     2881 gccgcctcgg cggcgctgaa gtagacctcg gtccggttgc acgtcgaaag aatcgcagct
     2941 tccttgacgc tcggctgcgc gatcaggtcg cgaagcgcga cgcccagcgt atccggctga
     3001 aatgcgacct gctcgcgcac cttgaccggc gcggtcgcat ggttcaagcc aagggcgtag
     3061 agaggcatgg tgatcgattg caagtgactg atattttatc gcaatccggt atagcgtgcc
     3121 gtacggatgc gcgctttagg aagacagttc ggcaagatac ccgaaaaaag cgcggacctg
     3181 ttccgggttg tccagcgagg cgccgaagaa cgacgagggc gccggcggcg agcgccccgc
     3241 atcgtgcttg cggcaaaaac ggtcggcgta acgctgccat gtcgcgtcgt aattgccgcg
     3301 ggaagcgtgg accagcagca agtcgccgac gacgccgagt ctcgctcccg ccttcgcggc
     3361 acggtacgac cagtcgagat cggccaggtg gaagccatcg aagttcacgt cgtcgaaggg
     3421 gaccgcctgg aaggcgtcgc gtttgccggc aagcaggacg ccatcgagca gaacgatgtc
     3481 gccggcgacc gggcgcggat cgagcacatc aacctgccag ttggcgctgt cgggcacgcg
     3541 gtgcgtgatc cagccgcgca agtgaggatg gccgctccag ccgatcgccg gaccgtcact
     3601 gcgagtactt cccgcaacgc cgacgacatc gaacgcctga aggcgctcga acagtcgcac
     3661 ggcaaaatcg gacgcgagga tgtcgacgtc gtcgtgcgaa agaacgacga cgtcggcgac
     3721 gctgtgccga agcgcccagt tgtaagcctc tgcgagggag cgcgcgttgc ggatggacac
     3781 gatctcgtgg cgcaccgcgg cgaagagccg gcggtacagc gcgacagcac gctcgtgccg
     3841 gacatcgtcg atcgagcaga tgacgatgct gaccgcacga tcgggtgtaa cgggcccgat
     3901 cgccggtagc cgcgcgtcag
//
LOCUS       DATANS010000652         7387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_327729, whole genome shotgun sequence.
ACCESSION   DATANS010000652 DATANS010000000
VERSION     DATANS010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327729"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /gene="hflC"
                     /locus_tag="VHT22_09580"
     CDS             <1..678
                     /gene="hflC"
                     /locus_tag="VHT22_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEX3137849.1"
                     /translation="DPDRVTTSEKTPLKADFIAYWRITDVRKYYQSVTGDEETARRRL
                     AQTIRANLAEEINKRTLHEAISTEREKIMTTTRQKADADAKSIGVEVVDVRLRRVELP
                     DEVLAQVYQRMESERKRVANELRSLGAAESEKIRADADRQREVILADAYKQAQKTKGE
                     GDAKASATYASAYGQNPEFYSFYRSLEAYKTTFRSKGDVIVLDPSAEFFQYLKQPGGG
                     PPASRGK"
     gene            703..891
                     /locus_tag="VHT22_09585"
     CDS             703..891
                     /locus_tag="VHT22_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 family protein"
                     /protein_id="HEX3137850.1"
                     /translation="MPDAFVAACALVLVLEGLLPLVAPRLWRDTFRRITELSDGQLRF
                     IGLTSLLAGTAVLLIFHA"
     gene            884..1867
                     /locus_tag="VHT22_09590"
     CDS             884..1867
                     /locus_tag="VHT22_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX3137851.1"
                     /translation="MHSWILPEYIEDILPPEAETIERLRRTLLDHFRDHGFRLVQPPL
                     IEHLDSLLTGTGHDLELQTFKVIDPLSGRLLGVRADITPQVARIDAHLLNEPGITRLC
                     YAGSVLRTVVAGLAQTREVLQIGAELYGDPQIDADREVLMLLLSSLAAVGVRGLHLDL
                     GHVGVYRALANGAHIAGNASDSAMFAALRGKDVPAVAELAASLPAVWRDAFAALPTLY
                     GPADEVLAAARRRLPDTPSIALALSALETLAQAAGPEVAALHIDLADLRGYHYQTGAS
                     FSVFTEGEANAIGRGERYDGIGKAFGRARPATGFTLDLRQLAALSGVAGQN"
     gene            1882..3174
                     /locus_tag="VHT22_09595"
     CDS             1882..3174
                     /locus_tag="VHT22_09595"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX3137852.1"
                     /translation="MGRNVVVIGTQWGDEGKGKVVDWLTEHAQGVVRFQGGHNAGHTL
                     VIAGRKTVLRLIPSGILRPKVRVYIGNGVVLSPSALLQEIGELEAAGVEVRSRLSISP
                     ACPLVLECHVALDQAREASMGDTRIGTTGRGIGPAYEDKIARRAVRLQDLFYPDRFAA
                     KLKPLIEYHNFVLTQYFKRDAVPYQQTLDGALALAPALLPMVGDVAALLQDARRRGDS
                     LLFEGAQGALLDIDHGTYPYVTSSNCLAGAAAPGTGIGPQMLDYVLGIVKAYTTRVGT
                     GPFPTELTDEIGAGLAKRGNEFGSVTGRPRRCGWLDVAALKRSLQLNGVDGLCITKLD
                     VLDGMPSIRICTGYRVNGVAQPLLPIGADAVAECKPVYEDMSGWKESTLGVDSFDALP
                     APARAYLRRIEQLTEVPIAMVSTGAERDETILLHHPFH"
     gene            3181..3711
                     /locus_tag="VHT22_09600"
     CDS             3181..3711
                     /locus_tag="VHT22_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX3137853.1"
                     /translation="MATSLHISWQQYNILVERLALQVHESAWRFNQVICIARGGLRVG
                     DVLSRIYELPLAILSTHSYTAEGGTLRGQLVIAEHMTMTAPRLGDRVLLVDDMVDSGH
                     TLEAVDRELPQRFPHITDMRTAVVWYKGCSVFKPDYYVDYLPDSPWIHQPFEIYDTLR
                     PEELKARLAAATGAEQ"
     gene            3708..5108
                     /gene="asnS"
                     /locus_tag="VHT22_09605"
     CDS             3708..5108
                     /gene="asnS"
                     /locus_tag="VHT22_09605"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEX3137854.1"
                     /translation="MNALSCADILAGRAPQDVPVTLQGWVRTRRDSKAGISFVHLSDG
                     SGFHPVQVVAPNTLPNYASEVSRLTAGCAVEATGMIVPSPAKGQPFEMQASALKVVGW
                     VDDPDSYPIQPKPHTLEFLREVAHLRPRTNVIGAVSRVRHTIAQAVHRFFDQNGFVWI
                     NTPIITTSDAEGAGELFRVSTLDLANLPRTAQGKVDFGSDFFGREAFLTVSGQLNIET
                     YCMALSKVYTFGPTFRAENSNTSRHLAEFWMIEPEIAFADLSDNATLAEALLKHIFRT
                     VLEERADDMAFFEERIEKGVIAKLQGMIDAEFVRMEYEDAIGILEKATQKFEFPVRWG
                     MDLQSEHERYVSESYVRGPVILVNYPKEIKAFYMRMNDDGRTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDARMAAAGMDVEHYAWYRDLRRYGTVPHAGFGLGFERTIAYVTGLAN
                     VRDVIPFPRSPGNARY"
     gene            complement(5146..5559)
                     /locus_tag="VHT22_09610"
     CDS             complement(5146..5559)
                     /locus_tag="VHT22_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137855.1"
                     /translation="MRQAALTALFFLSCCVASSAGADEEKLPVQLAGTGSTTPESGKT
                     TTAPWSISIVSQDADGNLKGTLNWNGRSCQISAVAFTGTYRNGVLDISAPATSRTCGA
                     ITMMLTKSSGADLLLEGSARFHGSDTPAAVTLRPR"
     gene            complement(5660..5746)
                     /locus_tag="VHT22_09615"
     tRNA            complement(5660..5746)
                     /locus_tag="VHT22_09615"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5710..5712),aa:Leu,seq:cag)
     gene            5781..>7387
                     /locus_tag="VHT22_09620"
     CDS             5781..>7387
                     /locus_tag="VHT22_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HEX3137856.1"
                     /translation="MTFLSKKQRNIAATRGGSKTATNGATVPPRDQATAISRPGSRLS
                     SSGESASGRRARRARGDVAIPEREQILQALREQGVPMPAEQLAELLGIDGDAAREAFA
                     GRLAAMERDGQLMTNRKGQLCVVAKLDLVTGTVQGHPDGFGFLVPDDGSPDLFLSAKE
                     MHKTLHGDRATARIVGVDRRGRPEGAIVDVLERANREVVGRVHEERGIWYLVAENKRI
                     SQDILIPPDMRGSARPGQVVVAEVIEQPSAHREALAKVIEVLGNYTDPGMEIEIALRK
                     HDLPYRFSAAAQRQAGRFPHEVQNADRKGRTDLTALPLVTIDGETARDFDDAVYCERG
                     AKGFRLIVAIADVSHYVADGDGLDGDARDRGTSVYFPRRVIPMLPEQLSNELCSLKPA
                     VDRLCVACEMTISADGEIESYKFFPAVMHSRARLTYNQVWDWLSQPKARTREAAAVLP
                     HLQNLYALYKVLAAARARRGAIDFETIELELKFDAQGKIETIVPVMRNDAHKLIEECM
                     LAANVCTAEFLARHKHPVLYRVHEGPTP"
BASE COUNT         1316 a         2450 c         2417 g         1204 t
ORIGIN      
        1 gaccccgacc gcgtgacgac atccgaaaag acgccgctga aggccgactt catcgcctac
       61 tggcggatca ccgacgtgcg caagtactac cagagcgtga ccggcgacga agagacggcc
      121 cgccgccgtc tggcgcagac gatacgcgcc aatcttgccg aggagatcaa caagcgcacg
      181 ctgcacgagg cgatctcgac cgagcgcgaa aagatcatga cgacgacgcg gcaaaaggcc
      241 gacgccgacg ccaagtcgat cggcgtcgag gtcgtcgacg tcaggctgcg gcgggtcgag
      301 ttgcccgacg aagtgctcgc ccaggtctac cagcggatgg agtcggagcg aaaacgcgtg
      361 gccaacgagc tgcgctcgct gggcgcggcc gaatcggaaa agatccgcgc cgacgccgac
      421 cgccagcggg aggtcattct cgccgacgca tacaagcagg cgcagaagac caagggcgag
      481 ggcgacgcga aggcctccgc gacctacgca tccgcgtacg gacagaatcc ggagttctat
      541 tcgttttacc gcagtctcga ggcgtacaag acgacttttc gcagcaaggg cgacgtgatc
      601 gtgctcgatc cgagcgccga gttcttccag tacctgaagc agccgggcgg cggcccgccg
      661 gcctcgcgcg gcaagtaacg ctttcgcgac ggacgaagaa aagtgccgga cgcattcgtt
      721 gcggcatgcg cgctggtgct ggtgctcgaa gggttgctgc cgctggtggc gccccgactg
      781 tggcgcgaca cgttccgccg catcaccgag ctgtccgacg gacaattgcg cttcatcggc
      841 ctcacgtcgc tgctcgccgg aacggccgtt ctgctgatat tccatgcata gctggatcct
      901 tcccgagtac atcgaggaca tcctcccgcc cgaagcggag acgatcgagc ggctgcggcg
      961 caccctgctc gaccatttcc gcgatcatgg cttccggctg gtccagccgc cgctgatcga
     1021 acatctggat tcgctgctta ccggcaccgg gcacgacctc gagctgcaga cgttcaaggt
     1081 gatcgatccg ctgtcggggc gcctgctagg cgtgcgcgcc gacatcacgc cgcaggtcgc
     1141 gcgcatcgat gcccacctgc tcaacgaacc gggcatcacc cggctctgct acgccggcag
     1201 cgtgctgcgc accgtcgtcg cggggctcgc gcaaacgcgg gaggtcctgc agatcggcgc
     1261 cgagctgtat ggcgatcccc agatcgacgc cgaccgcgaa gtcctgatgc tgctgctgtc
     1321 gtcgctagcc gccgtcggcg tgcgcggcct gcacctcgac ttgggacacg tcggcgtcta
     1381 ccgggcgctc gccaatggcg cgcacattgc cggcaacgcc agcgacagcg cgatgttcgc
     1441 ggcgctgcgc ggcaaggacg tgccggccgt cgccgagctc gccgcgagcc tgcccgcggt
     1501 ctggcgcgat gccttcgccg cgttgccgac gctgtacggg cccgccgacg aggtgctcgc
     1561 ggcggcgcga cgccggctgc ccgacacgcc gtcgatcgcg ctcgcgctgt cggcgctgga
     1621 aaccctggcg caggcggcgg gaccggaagt cgcagcgctg cacatcgatc tggcagatct
     1681 ccgcggctat cactaccaga ccggcgcgag cttctcggtg ttcaccgaag gcgaagccaa
     1741 cgcgatcggc cgcggcgagc gctacgacgg catcggcaag gcgttcggcc gcgctcgtcc
     1801 cgcgaccggc tttacgctcg acctgcgcca gctcgccgcc ctgagcggcg tcgcgggcca
     1861 gaactgaaag cgatggcgcg catgggcagg aacgtcgtag tcatcggcac gcagtggggc
     1921 gacgaaggca agggcaaggt cgtcgactgg ctgaccgagc acgcgcaagg cgtcgtgcgg
     1981 ttccagggcg gccataacgc cggacacacg ctggtcatcg ccggcaggaa gacggtgctg
     2041 cggctgattc cgtccgggat tctcaggccg aaagtccgcg tctacatcgg caacggcgtc
     2101 gtgctgtcgc cgtcggcatt gctgcaggaa atcggcgagc tggaagcggc gggagtcgag
     2161 gtgcgctcgc ggctttcgat ctcgccagca tgtccgctgg tgctcgagtg ccacgttgct
     2221 ctcgaccagg cgcgcgaagc atcgatgggc gatacgcgca tcggcacgac tggacgcggc
     2281 atcggccctg cctacgaaga caagatcgcg cggcgcgcgg tgcgcctgca ggatctgttc
     2341 tatcccgatc gattcgccgc gaagctcaag ccgctgatcg aatatcacaa cttcgttctg
     2401 acgcaatact tcaagcgcga cgcggttccc taccaacaga cgctcgacgg cgcgctggct
     2461 ctcgcgccgg cgctgctgcc gatggtcggc gatgtcgctg cgttgctgca ggacgcgcgc
     2521 cgccgcggcg attcgctgct gttcgagggt gcgcagggcg cattgctcga catcgaccat
     2581 gggacctacc cgtacgtcac ctcgtcgaac tgcctggccg gcgcggcagc gccgggcacc
     2641 gggatcggcc cgcagatgct cgattacgtg cttggtatcg tcaaggcgta cacgacgcgt
     2701 gtcggcaccg gcccgtttcc gaccgagctt accgacgaga tcggcgcggg actcgccaag
     2761 cgcggcaacg aattcggctc ggtcaccggg cgccctcgcc gctgcggctg gctcgacgtc
     2821 gcggcgctga agcgttcgct gcagctcaac ggtgtcgacg ggttgtgcat caccaagctc
     2881 gacgtcctcg acggcatgcc gtcgatccgc atctgcaccg gctatcgggt caacggcgta
     2941 gcgcaaccgc tgctgccgat cggcgcggac gccgtcgccg aatgcaagcc ggtatacgag
     3001 gacatgtccg gctggaagga aagcaccctc ggcgtcgaca gcttcgacgc gctgccggcg
     3061 ccggcgcgcg cgtatttgcg ccgcatcgag caattgaccg aggtgccgat cgcgatggtc
     3121 tcgaccggcg ccgagcgcga cgagacgatc ctgctgcatc atccgttcca ctaaagttac
     3181 atggcgacga gcctgcacat cagctggcag caatacaaca ttctggtcga gcgccttgcg
     3241 ctgcaggtgc acgaatcggc gtggcgcttc aaccaggtga tctgcatcgc gcgcggcgga
     3301 ctgcgcgtcg gcgacgtgct gtcgcggatc tacgagcttc cattggcgat cctgtcgacg
     3361 cattcgtaca ccgccgaggg cgggaccctg cgcggacaac tggtcatcgc cgagcacatg
     3421 acgatgaccg cgccccggct cggcgatcgg gtgctgctgg tcgacgacat ggtcgactcc
     3481 ggccacacgc tcgaggcagt cgacagggag ttgccgcagc gctttcccca cattaccgac
     3541 atgcgcacgg cagtcgtctg gtacaaaggc tgttcggtgt tcaagcctga ctactacgtc
     3601 gattatctgc ccgacagccc gtggatacat cagccgttcg agatctacga cacgctccgg
     3661 ccggaggagc tgaaggcgcg cctggccgcc gcgaccggag ccgagcaatg aatgcgctga
     3721 gttgcgccga catcctcgcc ggccgcgcgc cgcaggacgt tccagtcacc ttacagggat
     3781 gggtccgcac gcgtcgcgac tcgaaagccg gcatctcatt cgtccatttg tccgacggat
     3841 cgggtttcca tccggtgcag gtggtcgccc cgaacacgct gcccaactat gcgagcgagg
     3901 tctcgcggct gaccgccggc tgcgcggtcg aagccaccgg catgatcgtt ccttcacctg
     3961 ccaaaggcca accgttcgag atgcaggcga gcgcgctcaa agtcgtcggt tgggtcgacg
     4021 acccggattc gtatccgatc cagccgaagc cgcacacgct ggagttcctg cgcgaagtgg
     4081 cgcacctgcg gccacgcacc aacgtcatcg gcgcggtcag ccgcgtccgt cacacgatcg
     4141 cgcaggccgt gcatcgcttt ttcgaccaga acggcttcgt ctggatcaac acgccgatca
     4201 tcacgacgtc ggacgccgaa ggcgcgggcg agctcttccg cgtgtcgacg ctggatctcg
     4261 ccaacctgcc gcgcacggcg caggggaaag tcgatttcgg ctccgacttc ttcgggcgcg
     4321 aggccttcct caccgtctcc ggacagctga acatcgaaac ctactgcatg gccttgtcca
     4381 aggtgtacac gttcgggccg acgtttcgcg ccgagaattc gaacaccagc cggcacctgg
     4441 cggagttctg gatgatcgag cctgagatcg cgttcgccga tctgtccgac aacgcgacgc
     4501 tcgccgaggc gctgctcaag cacatcttcc ggaccgtgct ggaagagcgc gccgacgaca
     4561 tggcgttttt cgaggagcgc atcgaaaagg gcgttattgc caagctgcag ggaatgatag
     4621 acgccgaatt cgtccgcatg gagtacgaag atgcgatcgg catcctcgag aaggctacgc
     4681 agaagttcga gtttccggtg cggtggggaa tggacctgca gtccgagcac gagcgctacg
     4741 tgtccgagag ctacgttcgc ggtccggtga tcctcgtcaa ctacccgaag gagatcaagg
     4801 cgttctacat gaggatgaac gacgacggcc gaacggtcgc cgcgatggat gtgctcgcac
     4861 ccggcatcgg cgaaatcatc ggcggcagcc agcgcgaaga gcggctcgat gtcctcgacg
     4921 cccggatggc cgcggcgggt atggacgtcg agcattacgc ctggtatcgc gacctgcgcc
     4981 gttacggcac cgtgccgcac gccggattcg gcttgggctt cgagcgcacg atcgcctatg
     5041 taaccgggct agccaacgtg cgcgacgtga tcccgtttcc gcgctcgccg gggaacgcgc
     5101 gctattgacg cggtattgac gcgctatgga gtcttgactc gctgctcagc gcggccgcaa
     5161 ggtcaccgca gccggtgtgt ccgaaccgtg gaacctggcg ctgccttcga gcaggaggtc
     5221 ggcgccgctg gacttggtca acatcatcgt gatcgcgccg caggttcggc tggtggccgg
     5281 cgcgctgatg tcgagcacgc cgttgcggta ggtgccggtg aatgcgacgg cggagatctg
     5341 gcagctgcga ccgttccagt taagcgtgcc cttgagattg ccgtcggcgt cctgcgagac
     5401 gatcgaaatc gaccacggcg cagtcgtcgt ctttcccgat tcgggagtcg tcgagccggt
     5461 gcccgccaac tgcaccggca atttttcttc gtcggctccg gctgacgacg ccacgcagca
     5521 gctcaggaag aacaacgcgg tcaatgcggc ctgtctcatc gaacggctct cccgagcgca
     5581 tgtcacaaaa acgaaagccc gcatggtcgc gggcgtgcat cgattgccgg cattcctccg
     5641 gccgggccgg atgctacgat ggtgcccaga agaggactcg aacctccaca gtgttgccac
     5701 cgctaggacc tgaacctagt gcgtctacca attccgccat ctgggctgta cgtcagacta
     5761 gccggtaatt ttacgaaaga gtgactttct tgtcaaaaaa gcaacgcaac atcgccgcga
     5821 cccgcggcgg atcgaagact gcaaccaacg gtgcgactgt gcctccgcgc gatcaagcga
     5881 cggcgatctc gcgacccggt tcgcgcctga gctcatccgg cgaatccgcg tccggtcgcc
     5941 gcgcccgccg cgcgcgcggc gatgtcgcga ttccggagcg cgaacagata ctgcaggccc
     6001 tgcgcgagca aggcgtgccg atgccggccg aacaactggc cgaacttctc ggcatcgatg
     6061 gcgacgccgc gcgcgaagcg ttcgccggcc gtctggcggc gatggagcgg gacggccagc
     6121 tgatgaccaa ccgaaagggc cagctttgcg tggtcgccaa gctcgacctg gtgaccggaa
     6181 cggtgcaagg ccacccggac ggcttcggat ttctcgttcc cgacgatggc agtcccgatc
     6241 tgttcctctc ggcgaaagag atgcacaaga cactgcacgg cgatcgagcg accgcacgga
     6301 tcgtcggcgt cgaccgccgc ggccgcccgg agggcgcgat cgtcgatgtg ctcgagcggg
     6361 ccaatcgcga agtcgtcggc cgcgtccacg aagaacgcgg catctggtac ctggtcgcgg
     6421 aaaacaagcg catcagccag gacatcctga tcccccccga tatgcgcggc agcgcgcggc
     6481 ccgggcaagt ggtcgtcgcc gaagtgatcg agcagccgtc ggcgcaccgt gaagctctgg
     6541 cgaaggtgat cgaggttctg ggcaactaca ccgatcccgg gatggagatc gagatcgcgc
     6601 tgcgcaagca cgacctgccc taccgttttt cagccgccgc gcagcggcag gcggggcgct
     6661 ttccgcacga agtgcagaac gccgaccgga agggccgcac cgatctgacc gctctgccgc
     6721 tggtgacgat cgacggcgaa accgcgcgcg acttcgacga cgccgtctat tgcgagcgcg
     6781 gcgccaaggg attccgcctg atcgtcgcca tcgccgacgt cagccactat gtcgccgacg
     6841 gcgacggcct cgacggtgac gcgcgcgatc gcggcacgtc ggtctatttt ccacggcgcg
     6901 tcattccgat gctgcccgag cagctgtcga acgagctgtg ctcgctcaag cccgcggtcg
     6961 accgcctgtg cgtggcgtgc gagatgacga tcagcgccga cggcgagatc gagagctaca
     7021 agttctttcc ggcggtaatg cattcgcgcg cgcggctcac ctacaaccag gtatgggatt
     7081 ggctgtcgca accaaaggcg cggaccaggg aagcggccgc ggtgttgccg cacctccaga
     7141 acctgtacgc gctctacaag gtgctggccg ccgcgcgcgc ccgccgcggc gcgatcgatt
     7201 tcgaaacgat cgagctcgag ctcaagttcg atgcgcaggg caagatcgag acgatcgttc
     7261 cggtcatgcg caacgatgcg cacaagctga tcgaggaatg catgctcgcc gccaacgtct
     7321 gcaccgcgga attcctggcg cggcacaagc atcccgtgct ctaccgcgtg cacgaagggc
     7381 cgacgcc
//
LOCUS       DATANS010000653         6344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_706439, whole genome shotgun sequence.
ACCESSION   DATANS010000653 DATANS010000000
VERSION     DATANS010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6344
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706439"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..573
                     /locus_tag="VHT22_09625"
     CDS             43..573
                     /locus_tag="VHT22_09625"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX3137857.1"
                     /translation="MHLHRGNLFLVGMPGSGKSTLGRLLAKRLGKAFYDADSELERRL
                     GVSIPVIFELEGEPGFRDREQAIIAEYVLHTHTIFATGGGAVLRQVNRDCLKQNGSVL
                     YLHATPATLWERTKRSKHRPLLQAPDPFERLKEIYAFRDALYRETADFVVESDQEQVI
                     RLAQRLEQQLRASAQA"
     gene            589..1662
                     /gene="aroB"
                     /locus_tag="VHT22_09630"
     CDS             589..1662
                     /gene="aroB"
                     /locus_tag="VHT22_09630"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX3137858.1"
                     /translation="MPTLTVALAERSYPIHIGAGLLARAGELLASPRPARAIIVTNPV
                     VAAHYLAPLQAALLRSGVRCEAVLVPEGEAHKDWATLYDVHTRLLELGAERSTLLVAL
                     GGGVIGDLAGLAAATYQRGMPLLQIPTTLLAQVDSSVGGKTAVNHPLGKNMIGAFHQP
                     SAVIIDTTTLATLPEREYVAGIAEVIKYGAACDATFFDWIESHVDRMLSRDADAIAHA
                     VHESCRIKAQVVARDEREAGERAVLNFGHTFGHAIEAATGYGTWLHGEAVAAGMVLAT
                     ALSQRVSGLGVDDANRLKRLIDRAGVPVAPPVLGFDRWMELMARDKKAESGAMRFVLL
                     KALGAASVRSGIDEADLRAVLAA"
     gene            complement(1730..3049)
                     /locus_tag="VHT22_09635"
     CDS             complement(1730..3049)
                     /locus_tag="VHT22_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137859.1"
                     /translation="MAPNGMIARWLRPALALFALGGLLALGGCGGGSGAPNNPYQPGP
                     PTPGPLSVLPPIITAFSNTPVTLTISGGAAPFFVVSSNPAVLPVAQSPSGNTIVLLPG
                     TVTVDTAVTITVQDAVGQRATSTVTIKAAPIFGTLTIKPSSSACGANICSGQTGTASV
                     TVTGPGGVGIPGRQVRFDVVDGDFAIQSNNPGAPLVPTLTAVSDGNGVATVFIQANLG
                     APTQPATLRATELTSGNQVNGQFTIVQTTALSVLPGDATITAPDNTGCLGGFTVVYTV
                     FGGTPPYSASTSVPNAVTIQNGVNIPFGGSFTAITTGVCANPATIIVVDATGQTTTAT
                     LHNLLGPAGPPPPVPPVTVDITPGSYTTASGTGCTGQTFGFIVSGGVAPYNVAVIGGT
                     ATPMVVPASPGSFNITGLTDSSGSHTIVVGDASSPQVTSNAKVTCKP"
     gene            3367..>6344
                     /gene="gltB"
                     /locus_tag="VHT22_09640"
     CDS             3367..>6344
                     /gene="gltB"
                     /locus_tag="VHT22_09640"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX3137860.1"
                     /translation="MYDPANEHDACGLGFIAHIKGNPSHAIVTQGLQILKNLTHRGAT
                     GADPLQGDGAGILVQLPDAFLRRACGRQGLTLPALGQYGVGMVFLPKEPASRMACEQE
                     IERAVAGEGQLLLGWRDVPTDNSGLSERTKDVEPVIRQVFIARGSNRIDQDALERKLY
                     IIRKKSGHAIQALKLKHGKEFYVPSMSSRTIVYKGMLLAHQVGEYYLDLRDPTMVSAL
                     AMVHQRFSTNTFPTWDLAHPFRLVCHNGEINTLRGNVNWIRARQQAISSTVLGDDLDK
                     IWPLIYDGQSDSASFDNCLELLLMGGYPLAHAMMLMIPEAWAGNPLMDEDRRAFYEYH
                     AALMEPWDGPAAMAFTDGRQIGATLDRNGLRPARYIVTDDDYVIMASEVGVLDIPERK
                     IIRKWRLQPGKMLLVDTEQGRIIDDAELKRTLATAKPYRAWIDRTRIALEDLPAPAPP
                     QRSKVSLLDRQQAFGYTQEDLKLILAPMALNGEEAVGSMGNDAALPVLSNRPKVLYAY
                     FKQLFAQVTNPPIDPIREELVMSLVTFIGPRPNLLAVDIAPDATDLPMRLEAGKPILT
                     ADEMEKIRHIGLFTGATFRSRELDICYPAAWGAAGMEAALASLCAHAEDAIRLGFNIL
                     IVSDRNVSSELMPIPALLATAAVHQHLVRKGLRTSTGLVVETGSAREVHHFALLAGYG
                     AEAIYPYLALETLAQLHEIVPEVSPEESAKRFVKAICKGLYKVMSKMGISTYQSYCGA
                     QIFEAVGLNSAFIDKYFTGTASNIQGIGLFEVSDEAYRLHQIAFGDSPVLRDALDAGG
                     EYQYRVRGEEHMWTPDSIAKLQHAARANSHSTYQEYAKLINDQSRRLMTLRGLFELKP
                     AGAPVPLDEVEPAAEIVKRFATGAMSLGSISTEAHTTLAVAMNRIGGKSNTGEGGEDP
                     ARQTRDANGDWRRSAIKQVASARFGVTSEYLVNAEDLQIKMAQGAKPGEGGQLPGHKV
                     YPWIAKVRYATPGVGLISPPP"
BASE COUNT         1142 a         2020 c         2095 g         1087 t
ORIGIN      
        1 ccgccgctcg ctccggccgc acgtatcaac taaaatgccc cgatgcatct gcatcggggc
       61 aatctttttc tggtgggcat gccgggcagc ggtaaatcga cgctcgggcg gctgctcgcc
      121 aagcgcctgg gcaaggcgtt ctacgatgcc gacagcgagc tcgagagaag gctcggcgta
      181 tcgatcccgg tgatcttcga attggaaggc gagcccgggt tccgcgatcg cgagcaggcg
      241 atcattgccg agtacgtgct gcacacccac acgatattcg cgacgggcgg cggcgcagtg
      301 ctgcgccagg tcaaccgcga ctgcctgaag cagaacggat cggtgctcta cctccacgca
      361 acgccggcga cgctgtggga acgcaccaag cgcagcaagc atcggccgct gctgcaggct
      421 cccgatccgt ttgaacggct gaaggagatc tacgcgtttc gcgacgcgct gtaccgcgag
      481 accgcggact tcgtcgtcga gtccgaccag gagcaggtca tccggctggc ccagcggctg
      541 gagcagcaac tgcgcgcatc ggcgcaggcc tagttcttcc atctgccgat gccgacgcta
      601 accgtcgcac tcgccgagcg cagctatccg atccatatcg gcgccggact cctcgcacgc
      661 gccggcgaat tgctggcatc gccgcgtcct gctcgcgcga tcatcgtcac caatcccgtc
      721 gtcgccgcgc actaccttgc gccgttgcag gcagcgctat tgcgctccgg cgttcgctgc
      781 gaggccgtgc tagtccccga gggtgaagcg cacaaggatt gggcgacgct ctacgacgtc
      841 cacacgcgtt tgctcgagtt gggcgccgaa cgttcaacct tgctggttgc gctcggcggc
      901 ggtgtcatcg gcgatctggc cggactcgct gcggcaacct accagcgcgg catgcccttg
      961 ctgcagattc cgaccacgct gctcgcgcag gtcgactcgt cggtcggcgg caaaacggcg
     1021 gtcaatcatc cgctgggcaa gaacatgatc ggcgcgttcc atcagccatc cgccgtcatc
     1081 atcgacacca cgacgctggc gacgctgccg gagcgcgaat acgtcgccgg catcgccgaa
     1141 gtcatcaagt acggtgcagc gtgcgacgcg acttttttcg actggatcga gagccacgtc
     1201 gaccgcatgc tctcgcgcga cgcggacgcc atcgctcatg cagtgcacga gagttgccgc
     1261 atcaaggccc aggttgtcgc gcgcgacgaa cgcgaggccg gcgagcgtgc ggtgctcaac
     1321 ttcgggcaca cgttcggtca tgcgatcgaa gcagcaaccg gttacggcac gtggctgcat
     1381 ggtgaggcgg tcgctgcagg aatggtgctt gcgactgcgt tgtcccagcg agtaagcggt
     1441 ctcggtgtcg acgacgcgaa tcgcctgaag cgcctgatcg atcgcgcggg cgtgcctgtc
     1501 gcgccgccgg tgctcggatt cgatcggtgg atggaactga tggcgcggga caagaaggcg
     1561 gaatcgggtg cgatgcgctt cgtgctgctg aaggcgctcg gggccgcttc ggtgcgcagc
     1621 ggcatcgatg aggcagacct gcgcgcggtg cttgcggcgt agcgtgcaaa caaaagcggc
     1681 gcccgaaggc gccgctgagt tgtgatccgg ccggatcggc agccagcgat tacggcttgc
     1741 aagtcacctt ggcgttcgag gtgacttgag gcgaacttgc atcccccaca acgatcgtgt
     1801 ggctgccgct cgagtcagtc aggccggtaa tgttgaagct gcccggtgac gccggcacta
     1861 ccatcggagt tgctgtaccc ccgatgacgg ccacgttgta cggtgctaca ccgccggaga
     1921 cgatgaatcc gaatgtctgt ccggtgcagc cggttcccga tgcggtggta tacgacccgg
     1981 gcgttatatc gaccgtgacc ggcggaacgg gcggcggcgg gccggcgggc cccaagaggt
     2041 tgtgcagcgt cgcggtagtc gtctgtccgg tcgcatcgac gacgatgatg gtcgccggat
     2101 tggcgcaaac gccggttgtg attgccgtga atgacccgcc gaacggaata ttgacgccgt
     2161 tctgaatcgt gacggcgttc gggaccgaag tgctggccga atacggcggt gtcccgccaa
     2221 atacggtata gaccacggtg aacccgccga ggcaaccggt gttgtcgggc gcggtgatcg
     2281 tggcatcgcc aggcaggacg gacagcgcgg tggtctgcac gatcgtgaac tggccgttga
     2341 cctgattgcc gctggtcaat tcggtggcgc gaagcgtagc cggctgtgtc ggcgcgccga
     2401 ggttcgcctg gatgaacacg gttgccacgc cgttgccgtc ggagacggca gtcagcgtcg
     2461 gcaccagcgg cgcgcccgga ttgttgctct gtattgcgaa gtcgccgtcg acgacgtcga
     2521 agcgcacttg tcgacccgga atgccgaccc cacccggtcc ggtgacggtc accgaagcgg
     2581 tgcccgtttg tcccgagcag atgttggcgc cgcacgccga ggagctgggc ttgatcgtca
     2641 gcgtgccgaa gatcggcgcc gcctttatcg tcaccgtcga tgtcgctctc tgaccgaccg
     2701 cgtcctgcac ggtgatcgtc accgcggtat ccacggttac ggtgcccgga agcaggacga
     2761 tcgtattgcc gcttgggctt tgcgcgaccg gcagaaccgc aggattgctg gatacgacaa
     2821 agaaaggcgc agctccgccg ctgatagtaa gcgtgacggg tgtattcgaa aaagcggtga
     2881 tgatgggcgg caaaacggac agcgggcccg gagtgggcgg cccaggttgg tagggattat
     2941 tcggggcgcc gctgccgccg ccgcaaccgc ccagggcgag caatccgccg agcgcgaaaa
     3001 gcgcaagtgc cggtcgcagc cagcgagcaa tcattccgtt tggcgccatg aaggaatccc
     3061 tgatgatgtt gtcctaaata tcttacccgg gccggctttt ttaaacgttg gtacaagcat
     3121 atagcccact ttgttcagat taaagcgcaa gttcagggct ggcgcaagtg cttttggcgg
     3181 acagggcatc ccgggcgtcg ccaggttgcc gcaatgcaac attatggcta gtatgaaaag
     3241 gtttcacccg ccgccttgag gcggacgcgg cagggcatgc cggaaggcgc gatcgggccg
     3301 gtaaaaggcg aggatccagt ggagcgacag cgacaggtgc ggcacctgcc cccggcgagg
     3361 cagggaatgt acgacccggc aaacgaacac gatgcctgcg gcctggggtt catcgcgcac
     3421 atcaagggca accccagcca cgccatcgtc actcagggcc ttcagattct caagaatctg
     3481 acccaccgcg gcgcgaccgg cgccgatccc ttgcagggcg acggcgccgg catcctggtc
     3541 cagctgcccg acgcgttcct gcgccgcgcc tgcggccggc agggcctgac gctgccggcg
     3601 ctcggtcaat acggtgtcgg catggtcttt ctccccaaag agccggcgtc gcgcatggcg
     3661 tgcgagcagg aaatcgagcg cgcggtcgcc ggcgaaggcc agttgctcct cggctggcgc
     3721 gacgtgccga ccgacaattc gggcctgtcc gagcgcacca aggacgtcga gccggtgatc
     3781 cggcaggttt ttatcgcccg cggctcgaat cggatcgatc aggacgcgct cgagcgcaag
     3841 ctctacatca ttcgcaagaa atccgggcat gcgatccagg cgctgaagct caagcacggc
     3901 aaggagttct acgtgccgtc gatgtcctcg cggacgatcg tctacaaggg catgctgctc
     3961 gcgcatcagg tcggggagta ctaccttgat ttgcgcgatc cgacgatggt ctcggcgctg
     4021 gcgatggtgc atcagcgctt ctcgaccaac accttcccga cctgggatct cgcgcatccg
     4081 ttccgcctcg tctgccacaa cggcgagatc aacacgctcc gcggcaacgt gaactggatt
     4141 cgggcgcgcc agcaggcgat ctccagcacg gtgctcggcg atgacctcga caagatctgg
     4201 ccgctgatct acgacggcca gtctgattcg gcgtcgttcg acaattgtct cgagctgctg
     4261 ctgatgggcg ggtatccgct cgcccacgcg atgatgctga tgattccgga agcgtgggcg
     4321 ggcaatccgc tgatggacga ggatcgccgc gcattctacg aataccatgc tgcgctgatg
     4381 gagccctggg acgggcctgc ggcgatggcg ttcaccgacg gtcggcagat cggcgcgacg
     4441 ctcgatcgca acgggctgcg gccggcgcgc tacatcgtca ccgacgacga ctacgtgatc
     4501 atggcctccg aggtcggcgt gctcgacatt ccggagcgga agatcatccg caagtggcgg
     4561 ctgcagccgg gcaagatgct gctggtcgac accgagcagg gccgcatcat cgacgacgcg
     4621 gagctgaagc gcactctggc taccgcgaag ccgtaccgcg cgtggattga ccgcacccgc
     4681 attgcgctcg aggatctgcc ggcgccggcg ccgccgcaac gctcgaaagt ctcgctgctc
     4741 gaccgccagc aggcattcgg ttacacgcag gaagacctca agctgatctt ggcgccgatg
     4801 gcgttgaacg gcgaagaggc ggtcggctcg atgggcaacg acgccgcact gccggtgctg
     4861 tcgaaccgtc cgaaggtcct gtatgcgtac ttcaagcaac tgttcgcgca ggtgaccaat
     4921 ccaccgatcg acccgatccg cgaagagctc gtgatgtcgc tggtgacgtt catcgggccg
     4981 cggccgaacc tgctcgctgt cgacatcgcg cccgacgcca ccgatcttcc gatgcgactc
     5041 gaggccggga agcccattct gaccgccgac gagatggaga agatccgtca catcggcctg
     5101 ttcactggcg ccaccttccg ttcgcgcgag ctcgacatct gctatcccgc ggcgtggggc
     5161 gcagcgggca tggaagcggc gctcgcgagc ctctgcgctc acgccgagga cgcgatccgc
     5221 ctcggcttca acatcctgat cgtgtccgac cgcaatgtct cttccgagct gatgccgatt
     5281 ccggcgctgc tcgcaaccgc cgccgtgcac cagcacctgg tgcgcaaggg gctgcgcacc
     5341 agcaccgggc tggtcgtcga gaccggatcg gcgcgcgaag tgcatcattt cgcactgctc
     5401 gccggttacg gcgccgaggc gatctacccg tatctcgcgc tggaaacgct cgcccagctg
     5461 catgagatcg tgccggaagt ctcacccgag gaatcggcca agcgcttcgt caaggcgatt
     5521 tgcaagggcc tctacaaggt gatgtccaag atgggcatct cgacctatca gtcctactgc
     5581 ggcgctcaga tattcgaggc agtcggattg aacagtgcct ttatcgacaa gtatttcacc
     5641 ggcacggcga gcaacatcca gggtatcggc ttgttcgagg tctccgatga ggcttaccgg
     5701 ctgcaccaga tcgcattcgg cgacagtccg gtgctgcgcg acgcgctcga tgccggcggc
     5761 gaatatcagt accgcgtccg cggcgaagag cacatgtgga cgcctgactc gatcgccaag
     5821 ctgcagcacg cggcccgagc caacagtcat tcgacctacc aggaatatgc gaagctgatc
     5881 aacgatcaga gccgccggct gatgaccttg cgcgggctgt tcgaattgaa gcccgcgggc
     5941 gcgccggtgc cgcttgacga ggtcgaaccg gcggcggaaa tcgtcaagcg cttcgccacc
     6001 ggcgcgatga gcctcgggtc gatctcgacc gaggcgcaca ccacgctggc cgtcgcgatg
     6061 aaccgcatcg gcggcaagtc gaacacgggc gaaggcggcg aggacccggc gcgccagacg
     6121 cgggacgcca acggcgactg gcggcgcagc gcgatcaagc aggtggcgtc ggcgcgcttc
     6181 ggcgtgacca gcgagtacct cgtcaacgcc gaagacctcc agatcaagat ggctcagggc
     6241 gccaagcccg gcgaaggcgg gcagctgccg ggccacaagg tctatccctg gattgccaag
     6301 gtccgctatg cgacgccggg cgtcggcctg atctcgccgc cgcc
//
LOCUS       DATANS010000654         1625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_116335, whole genome shotgun sequence.
ACCESSION   DATANS010000654 DATANS010000000
VERSION     DATANS010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116335"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VHT22_09645"
     CDS             <1..523
                     /locus_tag="VHT22_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX3137861.1"
                     /translation="IGLIACQWARALGVTMIGTVGSDEKAALAKTNGCIHTIVYTREN
                     FVERVKQLTGGKGVPVVYDGVGKDTFPASLDCLSPRGMFVSFGNASGPIAAFDILLLS
                     QKGSLYATRPTLATYTASRPAMLKMSEEMFSLVLGGKLANEPRQTYALKDAAQAHRDL
                     ESRKTTGATILLP"
     gene            complement(545..>1625)
                     /locus_tag="VHT22_09650"
     CDS             complement(545..>1625)
                     /locus_tag="VHT22_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX3137862.1"
                     /translation="WIVQANDSCIPILALDVPSGLDAETGVAQARTIRARATATFIAL
                     KPGLLTLDGPDYSGAISVHRLEAADSDMPATAGTRLDWAVLRTALPEVLLRSRRNAHK
                     GTYGTLGVVGGSDGMVGAPLLAARAALHLGAGKVLVGLAATDRLAVDWQQPELMLRGA
                     ADVLERTLDAIAIGPGLGTGETARHLLGGALRLATPMVIDADALTLIAADATLSAAVA
                     ARSAPTVLTPHPAEAARLAGVKTSDIQANRIEAALTLARRLNAGVVLKGAGTVLAYPD
                     GSWAINGSGNPGLASGGTGDVLAGMLGALLAQGIALGDAMPLAVCLHGAAADALVAQG
                     VGPLGLTASELAPAARRLLNASRAG"
BASE COUNT          265 a          562 c          558 g          240 t
ORIGIN      
        1 catcggcttg attgcctgcc aatgggcgcg cgcgctcggc gtcacaatga taggcaccgt
       61 cggctcggat gaaaaagctg cgctggccaa aacgaatggc tgcatccaca ccatcgtcta
      121 tacgcgagag aattttgtcg agcgcgtcaa gcagctgacc ggcggcaagg gcgtgccggt
      181 cgtctacgac ggtgtcggca aggacacgtt tccggcatcg ctcgactgcc tgtcgccgcg
      241 cggcatgttc gtgagcttcg gcaacgcgtc gggaccgatc gccgcgttcg acatcctgct
      301 cctgtcgcag aagggctcgc tctacgcgac ccggccgacg ctggccacct acacggcgag
      361 ccgcccggcg atgctcaaga tgagcgagga gatgttctct ctggtcctgg gcggcaagct
      421 cgccaacgag ccgcgccaga cctatgcgct caaggacgcc gcgcaggcgc atcgcgatct
      481 cgaatcgcgc aagaccaccg gcgcgaccat actgctgccg taacgcggac gggggcggcg
      541 cgcgctatcc ggcgcgtgag gcgttgagca atcggcgtgc ggcgggagcc agttccgacg
      601 ccgtaagacc gagcggcccg acgccttgcg cgaccagcgc atcggcggct gcgccgtgca
      661 ggcatacggc cagcggcatg gcatcgccga gcgcaatccc ttgcgcaagc agcgcgccga
      721 gcatgccggc aagtacgtcg ccggtgccgc cggaggcgag ccccgggttg ccgctgccat
      781 tgatcgccca gcttccgtcg ggataagcca gcacagtgcc cgcgcctttg aggacgacgc
      841 cggcgttgag ccgccgcgcc agcgtcagtg cggcttcgat ccgattcgcc tggatgtccg
      901 atgtcttgac gccggcaagc cgcgctgctt cggcgggatg cggcgtaagg accgtcggag
      961 cgcttcgcgc ggccaccgcc gcggacagcg tcgcatcggc ggcgatcaag gtcagggcgt
     1021 cggcgtcgat taccatcgga gtggccagcc gcagcgcgcc gccgagaaga tggcgagccg
     1081 tttcaccggt gccgagaccg ggaccgatgg cgatggcgtc gagcgttcgt tcgagcacat
     1141 cggccgcgcc gcgcagcatc aactcaggct gctgccaatc gaccgccagt cgatcggtcg
     1201 cggcgaggcc cacgagcact ttgcccgcgc ccaggtggag cgcggcacgc gccgccagca
     1261 gcggcgcacc caccatcccg tcgctgccgc cgacgacgcc cagcgtgccg taagtcccct
     1321 tatgcgcgtt gcggcgcgaa cgcaaaagca cttccggcag cgcagtgcgc agcaccgccc
     1381 agtcgagccg cgtgccggcc gtcgccggca tgtcgctgtc ggcagcctcg aggcggtgca
     1441 cgctgatcgc gccagagtag tcgggtccat ccagggtcag caggccgggc ttcaaggcga
     1501 tgaaagtcgc cgtggcgcgg gcgcggatcg ttcgcgcttg cgcgacacct gtctcggcat
     1561 cgagcccgct cggcacgtcg agcgccagga tcggtatgca gctgtcattg gcctgaacga
     1621 tccag
//
LOCUS       DATANS010000655         1654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_239888, whole genome shotgun sequence.
ACCESSION   DATANS010000655 DATANS010000000
VERSION     DATANS010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239888"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VHT22_09655"
     CDS             <1..360
                     /locus_tag="VHT22_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3137863.1"
                     /translation="DRILGFFPQLKSRLDTPADFLSGGEQQMVAVARALSGHVRVLLL
                     DEPFEGLAPAVVEELFEVFDKLRSEVTMIIVEHNLDLVLALANHAYVLERGSVVYEGA
                     ATELRENLDLRRRVLWM"
     gene            365..1303
                     /locus_tag="VHT22_09660"
     CDS             365..1303
                     /locus_tag="VHT22_09660"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEX3137864.1"
                     /translation="MDKTIAVIGAGLIGRAWAMVFARGGWAVRLTDSNAEQLAHARAF
                     IAASLDEQQRYGLVGDARAALARIEHAADIESAIEGAEWVQENLPEKVEVKRDVFALL
                     DRHARPDAVLASSTSAIPASRFTESLAGRARCLVAHPVNPPHLVPIVELCGAPWTAPA
                     TIEHAERVFASLGQVPIVVRRELDGFVLNRLQGALLSEAMRLVGEGYVSADDLDKTVR
                     DGLGLRWSFLGPMATIELNAPGGVADYCARYGDFFRRLTADPAGPDVWDAANAARVAA
                     ALGAPRSPEAREARTVWRDRRLMALVAHKREQPDSE"
     gene            1315..>1654
                     /locus_tag="VHT22_09665"
     CDS             1315..>1654
                     /locus_tag="VHT22_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014717460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX3137865.1"
                     /translation="MAARQGRSVIITCAITGSIHTPSMSPYLPVTPQEIADAAVGAAE
                     AGAAIVHLHARDPIDGHPTQDPKYFRVFAADIARRSDVVINFTTGGAATMTIEERLQP
                     ALQLKPEVASL"
BASE COUNT          274 a          527 c          584 g          269 t
ORIGIN      
        1 gacaggattc tcgggttctt cccgcagctc aaatcgaggc tcgacacacc ggccgacttc
       61 ctgtccggcg gcgagcagca gatggtggcg gtcgcgcggg cgctgtcggg ccacgtccgc
      121 gtgctgctgc tggacgagcc gttcgaagga ctggcaccgg cggtcgtcga ggaactgttc
      181 gaggtcttcg acaagttgcg aagcgaggtt acgatgatca tcgtcgagca taacctcgac
      241 cttgtgctgg cgctggcgaa ccacgcctat gtgctggagc gcggcagcgt cgtctacgaa
      301 ggtgcagcta cggagcttcg ggaaaatctc gacctgcggc gccgtgttct gtggatgtga
      361 ggcgatggac aagacgatag cagtcatcgg cgccggcctg atcggccgcg cctgggcgat
      421 ggtattcgcg cggggagggt gggcggtccg gctgaccgac agcaacgcgg agcagctggc
      481 gcacgcccgc gcgttcatcg cggcaagtct cgatgaacag cagcgatacg ggctcgtcgg
      541 cgacgcccgc gcggcgcttg cgcgcatcga gcatgcggcc gacatcgagt cggcgatcga
      601 gggtgccgag tgggtgcagg agaatctgcc ggaaaaagtg gaggtcaagc gcgatgtctt
      661 cgcgctgctc gatcgtcacg cccgcccgga cgccgtgctc gccagctcga cctcggcgat
      721 tccggcatcg cgatttaccg aaagcctggc cgggcgtgcg cgctgtctgg tcgcgcatcc
      781 ggtcaatccg ccgcatctgg tgccgatcgt cgagctgtgc ggcgcgccat ggacggcgcc
      841 ggcgacgatc gagcacgccg aacgcgtatt cgcgtcgctt ggccaggtcc cgatcgtcgt
      901 ccggcgcgag ctcgacggtt ttgtgctcaa tcggctgcag ggtgcgctat tgtcggaagc
      961 gatgcgcctg gtcggagagg gttacgtgtc ggccgacgac ctcgacaaga cggtgcgcga
     1021 cggactcggg ctgcgctggt cgttcctcgg gccgatggcg acgatcgaat tgaacgcacc
     1081 cggcggcgta gccgattact gcgcgcgcta cggcgatttc ttccgccgcc ttaccgccga
     1141 cccggcgggg ccggacgtat gggatgccgc caacgcggcg agagttgccg ctgcgctggg
     1201 cgcgccgcgc tcgcccgagg cgagggaagc caggaccgtg tggcgcgatc ggcgcctgat
     1261 ggcgctggtc gcccacaaac gcgaacaacc ggattccgaa taaggaggtc aacgatggca
     1321 gcgagacagg gacgcagcgt aatcatcacc tgcgcgatta ccggatcgat tcacacgcct
     1381 tcgatgtcgc cctatctgcc ggtgacaccg caggaaatcg ccgacgcggc ggtcggcgcg
     1441 gccgaagcgg gggcggcgat cgtccatctg cacgcgcgcg acccgatcga cggacatccg
     1501 acgcaggacc cgaaatactt ccgcgtcttc gcggctgaca tcgcccggcg cagcgacgtc
     1561 gtgatcaatt tcacgaccgg cggcgccgcc acgatgacca tcgaggagcg tctgcagccg
     1621 gcgctgcagc tcaagcccga ggtcgcgtcg ctca
//
LOCUS       DATANS010000656         1646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_536703, whole genome shotgun sequence.
ACCESSION   DATANS010000656 DATANS010000000
VERSION     DATANS010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536703"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VHT22_09670"
     CDS             <1..260
                     /locus_tag="VHT22_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1348 family protein"
                     /protein_id="HEX3137866.1"
                     /translation="YRLIKELWAFADDRIAVRFAYEWHDDSGQWYRSYGNENWEFDSD
                     GLMRRRIASINDLPITEADRKYRWPLGRRPDDHPGLSDLGL"
     gene            260..>1646
                     /locus_tag="VHT22_09675"
     CDS             260..>1646
                     /locus_tag="VHT22_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX3137867.1"
                     /translation="MAVAPPPKGEIKHLARLWRFLAPYKGRVGIAFIALVTAASCVLV
                     LGQGLRHVIDAGFGSRDPHLLNVALGAVVAVSLLLAAATWTRFYLMMSVGERVIADVR
                     QAAFAHVLTLSPAFFDASRTGEISSRLTNDSEQMRQVIGFGVSMFLRNGLMMVGALVM
                     LFATSPKLAAFIVLGVPATLIPILIMGRRVRRLSRVNQDRVADVTAHIDESLHEIRTV
                     QAYRHEERTYERFAGATEAAYEAGVDRIRVKAWLISLVMLIGFCAVGVILWIGGHDVF
                     DGRLTAGELSAFVFYAAIVAASAGVVSEVWGEIQRAAGATERLMELLDVKPALVVAQP
                     AIRLPARVTGAIRFDDVKFAYPTRLETTALGPVSFTIHPGERVALVGPSGAGKSTVFA
                     LILRFYDPSAGRVLIDGADIRHCEPRELRGAVALVPQEPVIFAASVLENVRFGRPAAT
                     DGEVRAACVAAQ"
BASE COUNT          237 a          523 c          580 g          306 t
ORIGIN      
        1 attaccggct catcaaggag ctctgggcct tcgccgacga tcgcatcgcg gtgcgtttcg
       61 cgtacgagtg gcacgacgat tccggccagt ggtaccgctc gtacggcaac gaaaactggg
      121 agttcgacag cgacggcctc atgcgccggc gcatcgcgag catcaacgac ctgccaatca
      181 cggaggccga ccgaaagtac cgctggccgc tgggccgccg gcccgacgat catccgggac
      241 tgtccgacct cggactgtga tggctgttgc gccgccgccc aagggcgaaa tcaagcacct
      301 cgcgcgcctg tggcgttttc tcgcgccgta caagggccgc gtcggcattg cgttcattgc
      361 gctggtgacg gcggccagct gcgtactcgt gctcgggcag gggctgcgcc atgtgatcga
      421 cgcgggcttc ggcagccgcg atccgcatct gctcaacgtc gcgctgggcg cggtcgtcgc
      481 ggtatcgctg ctcctggcag ccgcgacgtg gacgcgattc tatttgatga tgagcgtcgg
      541 cgagcgcgtg atcgcggacg tgcgccaggc cgcgttcgcg cacgtgctga cgctgtcgcc
      601 cgcgtttttc gacgcgtcgc gcaccggcga gatttcctcg cggctcacca acgacagcga
      661 gcagatgcgc caggtgatcg gcttcggcgt gtcgatgttc ctgcgcaacg ggctcatgat
      721 ggtgggcgcg ctcgtcatgt tgtttgcaac cagcccgaag ctcgccgcgt tcatcgtgct
      781 cggcgtgccg gcaacgctga tcccgatcct catcatggga cggcgcgtgc ggcggctctc
      841 gcgcgtgaac caggaccgcg ttgccgacgt gacggcgcac atcgacgaat cgctgcatga
      901 aatccgtacc gtgcaggcgt atcggcacga agagcgcact tatgagcgct tcgctggtgc
      961 gacggaagcg gcgtacgagg ccggcgtcga ccgcatacgt gtgaaagcgt ggttgatctc
     1021 gctggtgatg ctgatcggct tctgcgccgt cggcgtgatc ctgtggatcg gcggccacga
     1081 tgtgttcgac gggcggctta ccgccggtga gctttctgcg ttcgtgttct atgcggccat
     1141 cgtcgccgcg agcgccggcg tcgtgtccga agtctggggc gagatccagc gcgcggcggg
     1201 cgcgacggag cggctgatgg aactgctcga tgtgaaaccc gccttggtcg tcgcgcagcc
     1261 ggcaatccgc ctgccggcgc gcgtgactgg cgcgatacgg ttcgacgatg tgaagttcgc
     1321 ttatccaacg cgtctggaaa cgactgcgtt gggccccgtg tcgttcacga ttcacccggg
     1381 tgagcgcgtc gcgctggtcg gcccgtcagg cgcggggaaa tccactgtct tcgcgctgat
     1441 cctgcgcttt tacgatccgt cggcgggccg cgtcctcatc gacggcgccg acatccggca
     1501 ttgcgaaccg cgcgagctgc gcggcgcggt cgcgctggtg ccgcaggagc cggtgatttt
     1561 cgcggcgagc gtgctggaga acgtgcgctt cggacgtccg gcggcgacgg atggcgaagt
     1621 gcgcgcagcg tgcgtcgcgg cgcagg
//
LOCUS       DATANS010000657         3003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_495700, whole genome shotgun sequence.
ACCESSION   DATANS010000657 DATANS010000000
VERSION     DATANS010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495700"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..254)
                     /locus_tag="VHT22_09680"
     CDS             complement(48..254)
                     /locus_tag="VHT22_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HEX3137868.1"
                     /translation="MNDETLNLSIRKFLKMVGINSQREIEQAVARAIADGSIAGRESF
                     PAFVTLGVAGLKIDVRFDGEIRLE"
     gene            complement(309..1067)
                     /locus_tag="VHT22_09685"
     CDS             complement(309..1067)
                     /locus_tag="VHT22_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3137869.1"
                     /translation="MIRVLVILPLAMFAGAALAQESRLDAIQKSGALRVCTPGDYKPF
                     SLAKDATFEGLDVEIAQSMAKALGVEAKFFKTSWPKLMDDFIEKCDLAVGGISVTLDR
                     QKRAFFSEPYMVNGKAPITRCENVGKFQTIADIDKPGVTVIENPGGSNEKFARANFKQ
                     AKIVIFDDNTKIFDEILNRRADVMISESVEAVVQQRIKPGLCAVNPTKPLQYGEMAYL
                     LPRGDTVLKAWVDQWMHLAKANGEYDRIVEAWLK"
     gene            1220..1912
                     /locus_tag="VHT22_09690"
     CDS             1220..1912
                     /locus_tag="VHT22_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137870.1"
                     /translation="MKIRTLVTKRQYYGMEAVALRAAMARVLSRVAGLPAEAARVSRR
                     NLYQDFGLDAAAAQDTLDQMVADGLFQQPDERQGDFALTERFLEFATARVVEPLPRER
                     ARQLLGKVCLLATQINAEWKSNPLEIVALAPFGSYISPDKLLAELPLGIVVRPRSPSR
                     KARWTRTVSRRDGARQIRAAVRELSTFFHIRMVSDPKLLPQPFAVVFHDGGDEGADPS
                     PAPAEPAAASQA"
     gene            1973..>3003
                     /locus_tag="VHT22_09695"
     CDS             1973..>3003
                     /locus_tag="VHT22_09695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3137871.1"
                     /translation="MNAELQASYDEFPYLSLAFPQSHPDRLATLARLHRLEPAPVGEC
                     RVLEIGCAAGGNLIPMAAALPRSRFLGIDFSAVQVREGLADVAALGLTNIELEQVDLR
                     DLGEESGVFDYIIAHGVYSWVPATVQEKLLDVCARQLAAGGVAYISYNTLPGWNMRGV
                     VRDAMRYHTRQFPDARSRVQQARATLDFLAQATEGAQSPYALMLRSEAEELRRKQDYY
                     VLHDHLEEFNEALFFHQFAARAARHGLAFLAEADFRTMQIGGLPAPVARTLDRMAPDI
                     LQREQLTDFLRNRIFRQTLLVHEDAPIDRALTPERVQSMWVASAARAAGDDSDPSSAV
                     PGEFRMPDG"
BASE COUNT          496 a          989 c          953 g          565 t
ORIGIN      
        1 agatccgacc actggcgacg gtcggcccgg tcgcgcgcag ggcgctgcta ttcgagtctg
       61 atctcgccgt cgaatctgac gtcgatcttc aatcccgcta cgcccagcgt cacgaaggca
      121 ggaaaacttt cccggcccgc aatcgagccg tcggcgatcg cgcgcgcgac cgcctgctcg
      181 atttcgcgct gtgaattgat gccgaccatc ttcagaaact tgcggatgct gaggtttagc
      241 gtctcgtcgt tcattgcgga aactccctga cggccgacgc ggcgcgacgc cgcgatcgtt
      301 gtcacgcgtt atttgagcca ggcttcgacg atgcgatcgt attcgccgtt agccttggcc
      361 agatgcatcc attggtcgac ccaggccttt aagaccgtat cgccgcgggg cagcaggtag
      421 gccatttccc cgtattgcag cggcttggtc ggattgaccg cgcacaggcc gggcttgatt
      481 ctctgctgga cgaccgcctc gaccgactcc gagatcatga cgtcggcgcg gcggttgagt
      541 atttcgtcga agatcttggt gttgtcgtcg aagatgacga tcttcgcctg cttgaagttg
      601 gcgcgcgcga atttctcgtt gctgccgccc ggattctcga tgaccgtgac gccgggcttg
      661 tcgatgtcgg cgatcgtctg gaatttgccg acgttctcgc agcgcgtgat cggcgccttc
      721 ccgttcacca tatagggctc ggagaagaaa gcgcgcttct gccggtcgag cgtcactgaa
      781 attccgccga cggccaggtc gcacttctcg ataaagtcgt ccatcagctt cggccaactg
      841 gtcttgaaga atttcgcctc gacgccgagc gccttggcca tcgactgcgc gatctcgacg
      901 tcaaggcctt cgaaagtggc atccttggcc aggctgaagg gcttgtaatc gccgggcgta
      961 cagacgcgca gcgccccgct cttctggatc gcgtcgaggc gagattcctg cgcgagcgcg
     1021 gcgccggcga acatggccag cgggagaatg acgagtaccc tgatcattga cgtgctccca
     1081 tcgatttgcc gggtccggcg gtaccgggcg ccggctggtc aggcgattat agggcagcga
     1141 ccgggccggt aagggggacg attgccgggc cgccgggcct atgctattgt catgaatccc
     1201 cgatcgatgc ccgtgcgcga tgaagatccg gacgctggtc accaagcgac agtactacgg
     1261 catggaagcg gtcgcgctgc gcgccgcgat ggcgcgggtg ctctcgcgcg tcgccggact
     1321 gccagccgag gcggcgcgcg tcagccgacg caacctctac caggatttcg gcctcgacgc
     1381 ggcggcggcc caggacactc tcgaccagat ggtcgccgat ggactgttcc agcaacctga
     1441 cgagcgtcag ggcgatttcg cgctgaccga gcgcttcctc gaattcgcca ccgcccgggt
     1501 cgtcgaaccc ctgccgcgcg agcgcgcccg tcagttgctg ggcaaggtct gcttgttggc
     1561 aactcaaatc aatgccgagt ggaaatccaa tccgctggag atagtggcgc tggcgccatt
     1621 cggtagctac atcagtcccg acaagctgct ggccgaattg ccgctgggca tcgtcgtgcg
     1681 gccgcggtcg ccgtcgcgca aggctcgctg gacgcgcacg gtaagccggc gagacggcgc
     1741 gaggcagatt cgtgccgcgg tgcgtgagct ctcgaccttc tttcacatcc ggatggtcag
     1801 cgatccgaag ttgctcccgc agccgttcgc cgtcgttttt cacgacggcg gcgacgaagg
     1861 cgccgatccg tcgcccgccc cggccgagcc ggctgccgca tcacaggcct gaagcgcgcc
     1921 ccgtccgatc gcgacacggc gtcgcgccgc tgctgctaaa ctggccgccg cgatgaacgc
     1981 cgagttgcag gcaagctacg acgaattccc gtacctgagc ctggcttttc cgcagtcgca
     2041 tccggaccgg cttgcgacat tagctcggct gcaccgcctg gaacccgcac cggtcggcga
     2101 atgccgcgtg ctcgaaatcg gttgcgctgc cggagggaac ctgattccga tggcggctgc
     2161 cctgccgcgc tcccgcttcc tcggcatcga cttttccgcc gtccaggtgc gtgagggcct
     2221 tgccgacgtc gcggcgctgg gtcttaccaa catcgaactc gagcaggtcg acctgcgcga
     2281 tctcggcgaa gaatccggcg tattcgacta catcatcgcg cacggggtct attcgtgggt
     2341 cccggccacc gtgcaggaaa aattgctcga cgtctgcgca cggcagctgg cagccggcgg
     2401 cgtcgcctac atcagctaca acaccctccc cggctggaac atgcgcggcg tcgtccgcga
     2461 tgcgatgcgt taccacacgc gccagtttcc ggacgcccgc agccgcgtgc aacaagcccg
     2521 tgccacgctg gacttcctgg cgcaggcgac cgagggcgcg cagtcgcctt acgcgctgat
     2581 gctgcgttcg gaagccgaag agctccggcg caaacaggac tattacgttc tgcacgatca
     2641 tctcgaggaa ttcaacgaag cgcttttttt tcatcagttc gccgcacgtg cagcgcggca
     2701 cggcctcgcc ttcctcgcgg aagcggactt ccgcacgatg cagatcggcg gcctgccggc
     2761 gccggtcgcg cgtacgctcg accggatggc acctgacatc ctgcaacgcg agcagctgac
     2821 cgatttcctg cgcaaccgca ttttccgcca gacgctgctg gtgcacgagg acgcgccgat
     2881 cgatcgagcg cttacacccg agcgtgtgca gtctatgtgg gtcgcgtcgg cagcacgcgc
     2941 ggccggcgac gacagcgatc cgagttcggc ggtgccgggt gaatttcgca tgcccgatgg
     3001 cag
//
LOCUS       DATANS010000658         1540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_73595, whole genome shotgun sequence.
ACCESSION   DATANS010000658 DATANS010000000
VERSION     DATANS010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73595"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1159)
                     /locus_tag="VHT22_09700"
     CDS             complement(137..1159)
                     /locus_tag="VHT22_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137872.1"
                     /translation="MYKSEPDYVTGRRGRLDLYPWIQHPAFCRCDICPPNRRKEAARS
                     GKTLEEVWASPLRLRLRRDSPYPTNRKLSAAAALNLKAEEEKRLAEAVAKAEAEERAR
                     QNAVSFGTVVDAYRAHLRKEGKDWVRAESRIANIEAFIGRERDSAAVDIVWYRRLLDE
                     GELLKPETRRHYASTLIAMLNYARAERVIRSHQLEGVRVPQVLREDEPEPWTSRELGV
                     ILGPALDEYEQEQAAWNAIVAQDKANRGLRSPSFVPLRGFCLVGYFTLMRPKSNKALT
                     WEEITLDPVSRTGWFKLADHKNRRKGITARALHPQLVDYLFRIRPAQASGLVHPNPAT
                     FRHTGR"
     gene            1166..>1540
                     /locus_tag="VHT22_09705"
     CDS             1166..>1540
                     /locus_tag="VHT22_09705"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3137873.1"
                     /translation="MPYRSCDAGVRLRKARDRRGMTQAQLAEAIDAYPSQISNYENGE
                     KVPEGETMVALSDALEISLDELARGVPREGPDEVRDVRLRASVRELEASADRQLIDAA
                     VTTIDGLVALAHHAEFEARVAKR"
BASE COUNT          267 a          483 c          520 g          270 t
ORIGIN      
        1 atttggtgcg cgaaaatcgc cgcagacccc gcgaccaaaa agctgcaatg ttcccggctt
       61 tcgcgcgaat gttccacgga aaaggcgagc cgccgcttgc tcggttacac gctggaaggg
      121 aagaaggccg acttcttcaa cgtccggtgt ggcggaaggt cgcgggattg ggatggacca
      181 aaccggaagc ctgcgccggt cggatgcgga acagatagtc aacgagctgc ggatgcagcg
      241 cgcgcgccgt gattcccttg cgccggttct tgtggtccgc cagcttgaac cagcccgtac
      301 gggagaccgg atcgagcgtg atctcttccc acgtcagcgc cttgttgctc ttggggcgca
      361 tcagggtgaa gtaaccgacc aggcagaagc cgcgcagcgg cacgaaggac ggcgaacgta
      421 ggccgcggtt cgctttgtcc tgcgccacga tcgcattcca cgcggcctgt tcctgttcgt
      481 actcgtcgag cgcgggaccg aggatgacgc cgagctcgcg cgaggtccaa ggctccggct
      541 cgtcttctcg aagaacctgc ggcacgcgta cgccttcgag ctggtgcgag cggatgacgc
      601 gctccgcgcg cgcgtagttg agcatcgcga tcaacgtaga cgcgtagtga cggcgcgttt
      661 cgggcttcag cagctcgcct tcatcgagca agcggcgata ccaaacgata tccacggccg
      721 cgctgtcgcg ctcacgaccg atgaacgctt ctatgttcgc gatgcgcgac tcggcccgta
      781 cccaatcctt cccttccttg cgaaggtggg cgcggtacgc atcgacgacc gtgccgaagc
      841 tcacggcgtt ctgtcgtgcg cgctcttccg cctccgcctt cgcgaccgct tcggctaagc
      901 gcttctcctc ttcggctttc aggttcaggg ctgctgcagc ggacagcttt cgattcgtcg
      961 gatagggaga atcgcggcga agacgaagcc tcagcggcga ggcccaaacc tcctcgagcg
     1021 tcttgccgct gcgcgctgct tccttccgtc ggttcggcgg gcaaatgtcg cagcggcaga
     1081 acgccggatg ctggatccac gggtagagat cgagccggcc tcgccggccg gtcacgtaat
     1141 cgggctcgct cttatacatc ggcgtatgcc ttaccgttct tgcgatgccg gagtgcggct
     1201 gcggaaggca cgcgaccgcc gagggatgac gcaagcgcag ctcgccgaag cgatcgacgc
     1261 gtatccatcg cagatctcga actatgagaa cggcgagaag gtgcccgagg gcgaaacgat
     1321 ggtggcgctc tcggacgcgc tggagatcag cctcgacgag ctggcgcggg gcgtcccgag
     1381 ggaagggccg gacgaggttc gagacgtgcg gctgcgtgcg agcgtgcgcg agctcgaagc
     1441 gtcggccgat cgccagctca tcgacgcggc tgtgacgacg atcgacgggc tcgtggcgct
     1501 ggcgcaccac gccgagttcg aggcgcgcgt ggcgaagcgg
//
LOCUS       DATANS010000659         3329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_328235, whole genome shotgun sequence.
ACCESSION   DATANS010000659 DATANS010000000
VERSION     DATANS010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328235"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="VHT22_09710"
     CDS             <1..1314
                     /locus_tag="VHT22_09710"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX3137874.1"
                     /translation="LRAHRIALTLPQGPAYVSLDAALQEQALAARVPLPALDRYPEPL
                     AGAPPHDAVREAASLLESGKRPLMMLGRMSNDPKAFALRVALAERLSAVVLTDIKTGA
                     SFPTQHPLHPFPPSLYVGDEAARAIRDADVILSLDWIDLGGTLSQACGGELPFGQVIH
                     CSLDQYVHNGSNMDYQALPPTDLSILAPPDALVTALVTALGPRTAAQPKPWFVKAASV
                     VTADGEVRKAAADCMPLEALARVTTETLAPYHPSYIRLPLGWPGDYCRFAHPQDYIGF
                     DGGGGIGSGPGMTIGAALALRGGDRLPVAVLGDGDYLMGLTALWTGVHYRVPALIIVA
                     NNESFFNDELHQERMARLRGRPVENRWIGMRMSDPTIDLATLARGQGATAHGPIRSAD
                     ALAAAIAEGADAVRAGALVVIDARVAPEYSRAVSSSLLKHLPAQK"
     gene            1326..2819
                     /locus_tag="VHT22_09715"
     CDS             1326..2819
                     /locus_tag="VHT22_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX3137875.1"
                     /translation="MLQRADSPLDPRLPRHRGIYYGGAWHATSGGRETALSSPGTGES
                     LGTAVDASAQDVDRAVVAARQAFASWRDVPAQQRAAAIRKAAAILREHAEELAWIDAI
                     DGGNPLTAMLFDVGISADYMDYFAGLVTEIKGSTIPIGPGTLNYTVREPLGVVARIGA
                     FNHPLLFVAGKCGAPLAAGNVLIVKPAEQTPLSALRLAELWSDVFPPGVFSVVTGGRD
                     AGAALVAHPKVAKIGFIGSVGGGRAVMQGASATLKALTLELGGKNALIACADAAPADV
                     AEGVVRGMNFRYVTGQSCNSTSRVYLHDDIHDATLPEIVKRVAAIRIGLPTERDTEMS
                     CLASQAQFDKTITYIKLGLEGGARLAYGGKRPADPKLAHGFFVEPTVFADVTDDMRIA
                     REEIFGPVVSILRWSDESDLISRVNALDVGLTASIWTHDLDRAHRLASKVEAGYVWIN
                     GASTHHVGVPFGGFKQSGLGREESIEELLACTQLKNVNLTLRTPPLP"
     gene            2907..>3329
                     /locus_tag="VHT22_09720"
     CDS             2907..>3329
                     /locus_tag="VHT22_09720"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3137876.1"
                     /translation="MPARRVKLSASAIMPVLGMLFAAAAMAENIAVGNYGSSANGMPF
                     AVALEKGYFQQEGANVTGIIASEGGGTSVRNAMAGVAYGEANPGAIAVAIQQGADIKI
                     VSDNVLTIAEFAWLVKKDSPIKTIQDLKGKKIGYTNPRS"
BASE COUNT          548 a         1117 c         1118 g          546 t
ORIGIN      
        1 ctgcgcgcgc atcgcattgc gctgaccttg ccgcaggggc ccgcttacgt ctcgctcgat
       61 gccgcgttgc aggaacaggc gctggccgcg cgcgtcccgc tgcccgcgct cgaccgttac
      121 ccggaaccgc tggccggcgc gccgccgcac gatgcggtgc gcgaggcggc gagcctgctc
      181 gaaagcggca agcgcccact gatgatgctc ggccgcatgt cgaacgatcc gaaggcgttc
      241 gcgctgcgcg tcgcgcttgc cgagcgcctt tccgctgtgg tgctgaccga catcaagacc
      301 ggcgcttcgt ttccaacgca gcatccgctg catccgtttc cgccgagcct ctacgtcggc
      361 gacgaggcag cacgcgcgat ccgcgacgcc gatgtgatcc tttcgctcga ctggatcgat
      421 ctcggcggca ccctgtcgca ggcttgcggc ggcgagctgc cgttcggcca ggtgattcac
      481 tgctcgctcg atcaatacgt gcacaacggg tcgaacatgg attatcaggc gctgccgccg
      541 accgatctgt cgatcctggc cccgcctgac gcactagtca cggctctggt aacggcgctc
      601 ggcccgcgca cggcggcgca gcccaagccg tggtttgtca aagccgccag cgtagtgacc
      661 gctgacggcg aagtgcgcaa agctgccgcc gattgcatgc cgctcgaagc attggcgcgg
      721 gtgacgaccg aaacgctcgc gccgtaccac ccgtcgtata tccgattgcc gttgggctgg
      781 cccggcgact actgccgctt cgcgcatccg caggactaca tcggcttcga cggcggcggc
      841 ggtatcggct cggggccggg gatgacgatc ggcgccgcgc tggcgttgcg cggcggcgac
      901 cggttgccgg tcgcggtgct cggcgatggc gactatctga tgggtctgac cgcgctttgg
      961 accggcgtgc attatcgcgt gccggcactg atcatcgtcg ccaacaacga atcgttcttc
     1021 aacgacgaac tgcaccagga gcgcatggcg cgcctgcgcg gacggccggt cgagaatcgc
     1081 tggatcggca tgcgcatgag cgacccgacg atcgacctgg cgacacttgc ccgcggtcag
     1141 ggtgcgaccg cccatggtcc gatacgcagc gccgacgcgc ttgccgcggc gattgccgag
     1201 ggcgccgacg ccgtgcgcgc gggcgccctg gtggtgatcg atgcgcgcgt cgcacccgag
     1261 tattcgcgtg cggtgtcatc gtcgctgctc aagcatctgc cggcccagaa gtgacgccga
     1321 acccgatgct ccaacgcgcc gacagtcctc tcgatccgcg gttgccgcgg catcggggta
     1381 tctattatgg cggtgcatgg cacgcgactt cgggtgggcg ggaaaccgcg ctctcgagtc
     1441 cgggaaccgg cgaatcgctc ggcaccgccg tcgacgcatc ggcgcaggac gtcgatcgcg
     1501 ccgtcgtcgc ggcgcggcag gcgttcgcgt cctggcgcga cgtgccggcg cagcagcggg
     1561 cggcggcgat acgcaaggcg gccgcaattc tgcgcgagca cgccgaggag ctcgcctgga
     1621 tcgacgccat cgacggcggc aacccgctga cggcaatgct gttcgacgta ggcatcagcg
     1681 ccgattacat ggactacttc gccggcctgg tcaccgagat caagggcagc acgataccga
     1741 tcggcccggg aacgctgaac tacacggtgc gcgaaccgct cggcgtggtc gcgcgcatcg
     1801 gtgcgttcaa ccatccgctg ctcttcgtcg ccggcaaatg cggcgcgccg ctcgctgcgg
     1861 gcaacgtgct gatcgtcaag ccggccgagc agacgccgtt gtcggcgctg cggctcgccg
     1921 agctctggtc ggacgtgttt ccgcccggcg tgttcagcgt cgtcaccggc ggacgcgacg
     1981 ccggcgccgc gctggtcgcg catccgaagg tggccaagat cggctttatc ggcagcgtcg
     2041 gcggcggacg tgcggtgatg cagggcgcga gcgcgacact caaggcgctg accctggagc
     2101 tcggcggcaa gaacgcactg atcgcctgcg ccgacgccgc gcctgccgac gtcgcggaag
     2161 gcgtggtccg cggcatgaat ttccgctacg tcaccggcca gtcgtgcaat tcgaccagcc
     2221 gtgtctacct gcacgatgac attcacgacg cgaccttgcc cgagatcgtc aagcgggtcg
     2281 ctgcgatcag gatcggactg cctaccgagc gcgacaccga gatgtcgtgc ctggctagcc
     2341 aggcgcagtt cgacaagacg atcacctata tcaagctggg gctcgaaggc ggtgcgcggc
     2401 ttgcctacgg cggcaagcgg ccggccgacc cgaagctcgc tcacggattc ttcgtcgagc
     2461 cgacggtgtt cgccgatgtg acggacgaca tgcgcatcgc tcgcgaggag atcttcggac
     2521 cggtggtcag tatcctgcgc tggtccgacg agagcgacct catctcacgc gtcaacgcac
     2581 tcgacgtcgg cctgaccgcg tcgatctgga cacacgacct cgaccgcgcg catcggctcg
     2641 cctcgaaggt cgaagccggc tacgtctgga tcaacggcgc cagcacgcac catgtcggcg
     2701 tcccattcgg cggattcaag caatcgggtc tcgggcgcga ggaatcgatc gaggagctgc
     2761 tcgcctgcac ccagcttaaa aatgtgaacc tgacgcttcg tacgccgccg ctaccgtgac
     2821 tggtcggacg ggctgcggct ttcgccggcc gcgtctgcct ggcaacgaac gttgcggatc
     2881 ggaattcaaa tccataggag gatgaaatgc ccgctcgtcg agtcaagctg tccgcttcag
     2941 cgatcatgcc cgtgctcggg atgctgtttg ccgccgcggc aatggccgag aacatcgccg
     3001 tcggaaatta cggcagctcg gctaacggca tgccgttcgc cgttgctctc gagaaaggct
     3061 acttccagca ggagggcgcc aacgtcaccg ggatcatcgc ctcggaaggc ggcgggacgt
     3121 cggtacggaa cgcgatggca ggcgtcgcct acggcgaagc caatccgggc gcgatcgcgg
     3181 tggcgatcca gcagggcgcc gacatcaaga tcgtcagcga caacgtgctc acgatcgccg
     3241 agttcgcgtg gctggtgaag aaggactcgc cgatcaagac gatccaggat ctgaagggca
     3301 agaagatcgg ctacaccaat ccccggtcg
//
LOCUS       DATANS010000660         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_788204, whole genome shotgun sequence.
ACCESSION   DATANS010000660 DATANS010000000
VERSION     DATANS010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788204"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VHT22_09725"
     CDS             <1..389
                     /locus_tag="VHT22_09725"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX3137877.1"
                     /translation="PAVYGHDTLRDINARLETHARAAGIRLLTFQSNHEGALVDRVQA
                     ARNESVRYVLINPAAFTHTSVALRDALAAVAIPYIEIHLSNVHAREPFRHHSYFSDKA
                     VGTIVGLGPVGYELALQFALRQLKAS"
     gene            413..850
                     /gene="accB"
                     /locus_tag="VHT22_09730"
     CDS             413..850
                     /gene="accB"
                     /locus_tag="VHT22_09730"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX3137878.1"
                     /translation="MDLRKLKTLIELVESSGIAELEIAEGEERVRITRALAPAAHHHP
                     LPAQHATPMQMAHVPVPAVVQEPEGHTIKSPMVGTFYRSASPGAKAFVDIGDSVQAGD
                     PLCIIEAMKLMNEIEADRAGVVKAILAENGQAVEYGQPLVIIG"
     gene            950..2305
                     /gene="accC"
                     /locus_tag="VHT22_09735"
     CDS             950..2305
                     /gene="accC"
                     /locus_tag="VHT22_09735"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX3137879.1"
                     /translation="MFDKVLIANRGEIALRIQRACREMAIKTVVVHSEADADAKYVRL
                     ADESVCIGPPPSAQSYLNIPAIISAAEVTDAQAIHPGYGFLSENADFAEKVEKSGFVF
                     IGPRPDTIRLMGDKVNAKIAMAKAGIPIVPGSEGALPDDPKEILKIARTVKYPVIVKA
                     AGGGGGRGMRVVHTEAALLPAVSLTRSEAGAAFGNPTVYLEKYLDKPRHIEIQVLADE
                     HKNVVYLFERDCSMQRRHQKIIEEAPAPGIALRLLTRIGERCVEACRKINYRGAGTFE
                     FLYQDDEFFFIEMNTRIQVEHPVSELITGIDIVQQQIRVAAGQRLTIRQRDVVRRGHA
                     IECRINAEDPDTFAPSPGRITSYHAPGGPGIRVDSHVYHNYFVPPNYDSMIGKVIAYG
                     DTREQAIARMRVALSEMVVEGIKTNIPLHQELLLDDSFLRGGITIHYLEQRMAKRKQT
                     A"
BASE COUNT          453 a          768 c          752 g          395 t
ORIGIN      
        1 agccggcagt ctacgggcac gacacgctgc gggacatcaa tgcgcggctc gagacgcacg
       61 cgcgagcggc cggtattcgc ctgctgacct tccagagcaa ccatgaaggc gcgctggtcg
      121 atcgggttca ggccgcccgc aacgagtctg tgcgctacgt attgatcaat ccggcggcgt
      181 ttacccatac cagcgtcgcg ctgcgcgacg cattggcggc cgtggcgatt ccctatatcg
      241 aaatccacct gtccaacgtc catgcccgcg agccgttccg gcatcactcg tatttctcag
      301 acaaggcagt gggcacgatc gtcgggctgg gaccggtggg ctacgagctg gcgctccaat
      361 ttgcgctgcg ccagctcaag gcgagttagc tcctgagccc gagggacacg cgatggatct
      421 gcgcaaactc aagacactga tcgagctggt cgaaagctcg ggcatcgcgg agctcgaaat
      481 cgccgagggc gaggagcggg tccggatcac gcgcgcgctg gcacctgccg cccatcacca
      541 cccgctgccg gcgcagcatg cgaccccgat gcagatggcg catgtgcctg tgccggcggt
      601 ggtgcaggaa cctgaaggcc acacgatcaa atccccgatg gtcgggactt tctatcgctc
      661 cgcctccccc ggtgccaaag cctttgtcga catcggcgac agcgtgcagg ccggcgatcc
      721 gctttgcatt atcgaggcga tgaagctgat gaacgagatc gaggccgacc gggccggcgt
      781 ggtcaaggcg atcctggccg agaatgggca ggccgtggaa tacggccagc ctttggtgat
      841 catcggctga cgtcgacgct gccggcgccc gattcgctcg acggcgaatc gcgcggcccg
      901 cgtcgcgcct gcctatttcc cgcccgtggc aaacaaatcc gccggacagc tgttcgacaa
      961 ggtgctgatc gccaatcgcg gtgaaatcgc gttgcgcatc cagcgcgcgt gccgcgagat
     1021 ggccatcaag accgtcgtcg tccattcgga agccgacgcg gacgccaaat acgtgcgcct
     1081 ggccgacgag tcggtatgca tcggccctcc gccgtcggcg cagagctact tgaacattcc
     1141 tgcgatcatc agcgcggccg aagtcaccga cgcgcaggcg attcatcccg gctacggctt
     1201 tctgtcggaa aacgccgact ttgccgagaa agtggaaaag agcggcttcg tctttatcgg
     1261 acctcgaccg gataccatcc ggctgatggg cgacaaggtc aacgccaaga ttgcaatggc
     1321 caaggccggc atccctatcg tcccgggctc cgaaggtgcg ctgcccgacg acccgaagga
     1381 aatcctcaag atcgcccgga ccgtcaagta cccggtgatc gtcaaggccg ccggtggcgg
     1441 cggcggtcgc ggcatgcggg tcgtgcatac cgaggccgcg ctgcttcccg cggtcagcct
     1501 gacccggtcg gaagcgggcg cggcgttcgg caacccgact gtgtatctgg aaaaatatct
     1561 ggacaagccg cgccacatcg aaatccaggt gctggccgac gagcacaaga acgtcgtcta
     1621 cctgttcgag cgcgactgct cgatgcagcg ccgccatcag aagatcatcg aggaggcgcc
     1681 cgcgccgggg atcgcgctgc gtctgctgac gcgcatcggc gagcgctgtg tggaagcctg
     1741 tcgcaaaatc aattaccgcg gcgcgggcac gttcgagttc ctgtaccagg acgacgaatt
     1801 cttcttcatc gaaatgaaca cgcgcatcca ggtcgagcat ccggtgagcg agttgatcac
     1861 cggcatcgac atcgtccagc agcagatccg cgtcgccgcc ggccagcggc tgacaatccg
     1921 gcagcgcgac gtcgtccggc gcgggcacgc gatcgaatgc cggatcaacg ccgaggatcc
     1981 ggacacgttc gcgccttcgc cggggcggat cacttcgtat cacgcgccgg gcgggccggg
     2041 gatacgagtc gattcgcacg tctatcacaa ttatttcgtg ccgccgaact acgactcgat
     2101 gataggcaag gtgatcgcgt acggcgacac gcgcgagcag gcgatcgccc gcatgcgcgt
     2161 cgcgctgtcg gaaatggtcg tcgaaggcat caagaccaat attccgctgc atcaggagct
     2221 gctgctcgac gattcgttcc tgcgcggcgg gatcacgatt cactacctcg agcagcggat
     2281 ggccaagcgc aagcagacag cataggccga gcatcgccga tgccgtttgt cgcattgcgc
     2341 ttcgacgcgc ccgccgcgac tgccgacg
//
LOCUS       DATANS010000661         2554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_827764, whole genome shotgun sequence.
ACCESSION   DATANS010000661 DATANS010000000
VERSION     DATANS010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827764"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /locus_tag="VHT22_09740"
     CDS             complement(<1..1370)
                     /locus_tag="VHT22_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX3137880.1"
                     /translation="MTYAELHCISNFSFLRGASHPEELVARAKEEGYAALAITDECSL
                     AGVVRAHIEAKKCALKLVIGSEFRLDDGLRVVLLATDRKSYGNLAALITRGRRSAVKG
                     SYRLSRADLADGVAGCLALWLPSRVPDADEARWLKPRFREALWIAVERLLEADDAARC
                     AALAEIASMTGLPLVAAGDVHMHGRDRRALQDTLTAIRLKTTVAQAGHALHPNGERHL
                     RPLATLERIYPPAWLAETLAIAGRCRFSLDELRYEYPEEIVPAGETPAAYLRRLTEEG
                     FARRFPAAGTPLKDRAKVRALIERELALIAEKHYEAFFLTVYDIVKFARDHDILCQGR
                     GSAANSAVCYCLGVTEVNPSCTEVLFERFISRERDEPPDIDVDFEHQRREEVIQYIYR
                     KYGRDRAALTATVICYRPRSALRDVGKALGLDLSQVDRLAKAVGWWDGCQRLPQRIGD
                     AGLDET"
     gene            complement(1384..>2554)
                     /locus_tag="VHT22_09745"
     CDS             complement(1384..>2554)
                     /locus_tag="VHT22_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX3137881.1"
                     /translation="WGGQWTSTISIEPPAVVLLEISACLNYFEGLPKLLARIDAGLAA
                     LGFSGVIATAPTALAASLLARAGQAIAIEHTVDLEPRLGTLPVALLDSERPILDTLTG
                     IGVHTIADVLALPRDGVARRFGQRLLDEIDRARGIQPDPRSLFVPPERYHGQLELPVP
                     VAEAEALLFAAKRLVVELAGFLQGRGAGVTRLRCDLIHEDAPPTAIVLGLAATRKVEH
                     IVNVLRERLAREELPDRVEAIRLVSEEIAPFPGQDGELFAGAHRHGESGKQWIERLRA
                     RLGDDSVRELCFNADHRPEHAWRYRGTTSASAVPLPADPPRRPLWLLPRARPLGVDLA
                     SAQLSLLSGPERIESGWWDGGEIGRDYFVGSDAQGGELWLYRDRGGQWFVHGIFS"
BASE COUNT          434 a          873 c          844 g          403 t
ORIGIN      
        1 ctcgtctcat ccagaccggc atcgccgatt ctttgcggca gccgttggca cccatcccac
       61 cagccgaccg cctttgccag cctgtccact tgcgacagat ccagccccag cgccttgccg
      121 acgtcgcgca gcgccgagcg cgggcgatag cagatcactg tggccgtcag cgccgcgcgg
      181 tcgcggccgt atttccgata gatgtactgg atcacctcct cgcggcgctg atgctcgaag
      241 tcgacgtcga tatcgggagg ctcgtcgcgc tcgcgggaaa tgaagcgctc gaacaatacc
      301 tcggtgcacg acggattgac ttcagtgacg cccaggcagt agcacaccgc cgagttcgcc
      361 gccgagccgc gtccctggca gaggatgtcg tggtcgcgcg caaatttgac gatgtcgtag
      421 acggtgagaa agaacgcttc atagtgcttt tcggcgatca gtgcgagctc ccgctcgatt
      481 agcgcgcgca ccttggcgcg atccttcagc ggcgtgcccg cggcgggaaa ccgtcgcgca
      541 aagccttcct cggtcagccg acgcaggtaa gcggccggcg tttcacccgc gggaacgatc
      601 tcctccggat actcgtagcg cagctcgtcc agcgagaatc ggcagcgccc ggcgatggcc
      661 agcgtctcgg ccagccacgc cggaggatag atgcgctcga gcgtggcaag cggccgcaaa
      721 tggcgctcgc cgttcggatg gagcgcgtgg ccggcttgcg cgaccgttgt cttcagccgg
      781 atggcggtca gcgtgtcctg caaggcgcgg cgatcgcggc catgcatgtg gacgtcgccg
      841 gcggcaacga gcggcagacc ggtcatcgac gcgatttcgg cgagagcggc gcaacgcgcc
      901 gcgtcgtcgg cctcgagcag gcgctcgacg gcgatccaca gcgcttctcg aaagcgcggc
      961 ttgagccagc gcgcttcgtc ggcgtcggga acccggctcg gcagccatag cgcgagacag
     1021 ccggcgacac cgtcggcaag atcggcgcgc gacaaacggt agctgccctt gactgcgctg
     1081 cgccttccgc gcgtgatcag cgcagccagg ttgccgtagc tcttccgatc ggtcgccagc
     1141 aggaccactc gcaagccgtc gtcgagacga aactcgctgc cgatgacgag cttcagtgcg
     1201 cattttttcg cttcgatgtg cgcccgaacg acgccggcga gcgagcattc gtcggtgatc
     1261 gccagcgccg cgtagccttc ctccttggcg cgcgcaacca gctcttccgg atgcgaggct
     1321 ccgcgcagga agctgaagtt ggaaatgcag tgcaattcgg cgtaggtcat gcggatcgcg
     1381 ccgctaggag aagatcccgt gtacgaacca ttgcccgcct cgatcgcggt agagccacag
     1441 ctcgcctccc tgcgcatcgc tgccgacgaa ataatcgcgg ccgatctcgc cgccatccca
     1501 ccagccgctt tcgatgcgct ccgggccgga gagcaagctc aactgcgcgg atgcgagatc
     1561 gacgccgagc gggcgcgccc gaggcaacag ccacagcggc cgtcgcggtg gatcggccgg
     1621 aagcggcacc gcgctcgccg atgtggtgcc gcgatagcgc caggcatgct cgggacgatg
     1681 atcggcgttg aaacagagct cgcgcaccga gtcatcgcca agccgtgccc gcaggcgctc
     1741 gatccactgc ttgccgctct cgccatgacg atgggctccg gcaaagagct cgccatcctg
     1801 ccccgggaac ggcgcgattt cttccgacac cagtcggatc gcctcgaccc ggtcgggcag
     1861 ttcttcgcgc gccaggcgct cgcgcagcac gttgacgatg tgctcgacct tgcgcgtcgc
     1921 tgcaaggccg agcacgatgg ctgtcggcgg cgcgtcctcg tgaatcaggt cgcagcgcag
     1981 tcgcgtgacg ccggcgccgc gcccctgcag gaatccggcg agctcgacga ccagtcgctt
     2041 ggcggcaaac agcagcgcct cggcttcagc caccggcaca ggcaattcga gctggccgtg
     2101 atagcgctcg ggcggcacga acagcgaacg cggatcgggc tggatgccgc gcgcgcgatc
     2161 gatttcgtcg agcagccgct ggccgaaccg ccgcgccacg ccgtcgcgcg gcagtgccag
     2221 cacgtcggct atcgtatgca cgccgatgcc ggtcagcgtg tccagaatgg ggcgctcgct
     2281 gtcgagcagc gcgacgggca gcgtcccgag tcgcggttcg agatccaccg tatgctcgat
     2341 cgcgattgcc tggccggccc gcgccagcag gctcgcagca agcgcggttg gcgcggtagc
     2401 aatgacgccg gaaaatccca gcgcggcaag gccggcatcg atgcgcgcga gcaacttggg
     2461 caagccttcg aaataattga ggcaggcgga gatctccagc agcaccaccg ccggtggttc
     2521 gatgctgatc gtcgacgtcc attgcccgcc ccat
//
LOCUS       DATANS010000662         1792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_369336, whole genome shotgun sequence.
ACCESSION   DATANS010000662 DATANS010000000
VERSION     DATANS010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369336"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1792
                     /locus_tag="VHT22_09750"
     CDS             <1..>1792
                     /locus_tag="VHT22_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX3137882.1"
                     /translation="LVSDIARRYHVEQELRESESRTSLIINAVKDCAIYMLDTEGRVA
                     SWNPGAQALNGYTATEIVGKHFSVLYPPDRKEPPEKELAVAARRGWYEEECWHLRKDG
                     SRYCGDDIISAIRDEPGNLRGFSIVTRDATMRLELREQTERARDFYFSLFSGFPNLVW
                     RSDTTGACDYLNQAWLEYTGRRQEEEFGSGWLDGVHPDDRARWREIIAETFPAQKTFE
                     YEFRMRRADGSFGSIICNGRPYYDMQGRFAGYLNACYDNTVRRAMESALKESEARYQG
                     MTANVPGMVFELLRDPAGKLWFSYVSEGVAALTGLTEAALTADSAAFFDLVAPAERPH
                     LEATLEASAAQLSNWSWSGRIQPRHESTERWISIRARPRQAENAGVLWDGVVFDDTQS
                     RLSQVELERSREELRELSRHLQTVREEEKQRIAREVHDELGATLSVLKMDLESLDAQL
                     PAHLSEARAKRSAMIQLVDSAVATTRKIVTDLRPSMLDDLGLAAALRWQIGEYEKHTK
                     LGLHLDTPNPDIAIDRERGLALFRIFQETVTNVARHAKATNAWIGLAQTDRAYVLTVR
                     DDGDGVSEEDLRKPMSHGVRGMRERAQHFGG"
BASE COUNT          359 a          559 c          592 g          282 t
ORIGIN      
        1 ggctggtcag cgacatcgcg cggcggtatc acgtcgagca ggaattgcgc gagagcgaaa
       61 gccgcaccag cctgatcatc aacgccgtca aggactgtgc gatatacatg ctcgacaccg
      121 aagggcgggt tgcaagctgg aatcccggcg cgcaggccct gaacggctat acggcgaccg
      181 agatcgtcgg caagcacttt tcggtgctct acccgcccga tcgcaaggag ccgccggaaa
      241 aagagctcgc tgttgcagcc aggcgcggct ggtacgagga ggaatgctgg cacctgcgca
      301 aggacggcag ccggtattgc ggcgacgaca taatttcggc gatccgcgat gagcccggca
      361 atcttcgcgg cttttccatt gtcacccgcg acgcgacgat gcgactcgag ctgcgtgagc
      421 agaccgaacg cgcgcgcgac ttttattttt cgctgttctc gggattcccc aacctggtct
      481 ggcgctccga caccaccgga gcctgtgact acctgaacca ggcgtggctc gagtacacag
      541 gcaggaggca ggaagaggag ttcggctcgg gttggctgga cggcgtgcat cccgacgatc
      601 gcgcccgctg gcgggaaatc atcgcggaaa cctttcccgc gcagaagacc ttcgaatacg
      661 agttcaggat gcgccgcgcc gacggcagct tcggatcgat tatctgcaat ggccgtccgt
      721 attacgacat gcagggacgc tttgccggct acctgaacgc ctgctacgac aacacggtgc
      781 ggcgcgcgat ggaaagtgcg ctcaaggaaa gcgaggcgcg ttatcagggc atgacggcca
      841 acgtgccagg catggtgttc gagttgctgc gcgaccccgc cggcaagctg tggttctcgt
      901 atgtcagcga gggtgtcgcc gcgctgaccg gtcttaccga ggcggcgctg accgcggaca
      961 gcgcggcgtt tttcgacctc gtcgcgcccg ccgagcgacc gcatctggaa gcaacgctcg
     1021 aagcatccgc cgcacagctt tcgaactgga gctggagcgg ccggatccag ccgcgccacg
     1081 agtcgaccga aagatggatc agtattcggg cccgtcccag gcaggcggaa aacgcgggcg
     1141 tgctttggga cggtgtcgtg ttcgacgaca cgcaaagccg tctgtcgcag gtggagctcg
     1201 agcgctcacg cgaggagctg cgcgagctgt cgcggcacct gcagacggta cgcgaggaag
     1261 agaagcagcg catcgcgcgc gaggtgcacg acgagctcgg cgcgacgctg agcgtgctga
     1321 agatggacct ggagtcactc gacgcgcagc tgccggccca cctctcggag gcgcgggcca
     1381 agcgcagcgc gatgatccag ctggtcgact ccgccgtggc cacgacacgc aagatcgtca
     1441 ccgacttgag acccagcatg ctcgatgacc tcggtctcgc cgcagcgctg cgctggcaga
     1501 tcggcgaata cgaaaaacac accaagctag ggcttcatct cgatacgccc aatccggaca
     1561 ttgcaatcga tcgcgaacgt ggattggcgc tcttccgcat attccaggaa acagtgacca
     1621 acgtggcgcg ccacgccaaa gcgaccaatg cctggatagg gctggcgcaa accgaccgcg
     1681 cctacgtact gactgtccgg gacgacggcg acggcgtcag cgaggaagac ttgcgcaagc
     1741 cgatgtcgca cggcgtccgg ggcatgcgcg agcgcgccca gcacttcggc gg
//
LOCUS       DATANS010000663         1647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_73894, whole genome shotgun sequence.
ACCESSION   DATANS010000663 DATANS010000000
VERSION     DATANS010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73894"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..108)
                     /locus_tag="VHT22_09755"
     CDS             complement(<1..108)
                     /locus_tag="VHT22_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEX3137883.1"
                     /translation="MNAPDLHQKDLYEANKLAKRMRRLVGQAIGDYAMIR"
     gene            complement(105..863)
                     /locus_tag="VHT22_09760"
     CDS             complement(105..863)
                     /locus_tag="VHT22_09760"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HEX3137884.1"
                     /translation="METTGTLTGKTALVTGGAKRVGAAICRRLHAAGASLMIHHRASI
                     AEARALQHELNARRAESVALIQADLLSGASLPDLVKTTIKQFGHLDILVNNASSFFAT
                     AIGEITDKEWDDLIGTNLKAPLFLSQAAAVELRKRHGCIVNIVDIHAELPMKNYLVYT
                     VAKGGLLALTRSLARELGPEVRVNGVAPGTILWPEDDAWSDELSRQRIVNQTALKRAG
                     EPDDIAKAVEFLVAGAPYVTGQVIAVDGGRSITL"
     gene            1058..>1647
                     /locus_tag="VHT22_09765"
     CDS             1058..>1647
                     /locus_tag="VHT22_09765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX3137885.1"
                     /translation="MSFARYMELALHAPELGYYSAGSTKFGAAGDFVTAPTLGRVFGR
                     TLARQAAQILHAGIPDLVELGAGDGRLARDLLAELEALGSLPQRYLILETSADLRARQ
                     RDLLRRDVPRLAGRVEWLDTLPASLTALVLGNEVLDAMPVHVIEVGAGGIAERGVTLA
                     GGEFQWQTRPATGTVLAAAQALALPPGYITEINLAA"
BASE COUNT          264 a          556 c          515 g          312 t
ORIGIN      
        1 gcggatcatt gcataatcgc caatcgcctg cccgaccagc cggcgcatcc gcttggcgag
       61 cttgttcgct tcgtacaagt ccttctggtg caggtctgga gcgttcatag cgtgatgctc
      121 cggccgccgt cgacggcgat cacctggccc gtcacgtagg gcgcgccggc gaccagaaat
      181 tcgaccgcct tcgcgatgtc gtcgggctcg ccggcgcgtt ttaacgcggt ctggttcacg
      241 atgcgctggc gcgacagctc gtcggaccac gcatcatcct ccggccacag gatggtgccg
      301 ggcgcgaccc cgttcacccg cacttccggg cccagctcgc gtgcgagcga gcgcgtcagc
      361 gcgagcagcc cgcccttcgc gaccgtgtac acgaggtaat tcttcatcgg caattcggca
      421 tggatgtcga cgatgttgac gatgcagccg tggcgcttgc gcaattcgac cgcggctgcc
      481 tgcgacagga acaacggcgc cttcaaattg gtgcctatga ggtcgtccca ctccttgtcc
      541 gtgatctcgc cgatcgcggt cgcgaaaaag ctcgatgcgt tgttcacgag aatatcgaga
      601 tgaccgaact gcttgatcgt cgttttgacc aggtcgggca ggcttgcgcc gctcagaaga
      661 tcggcttgga tcagcgccac cgattccgcg cggcgtgcgt tcaattcgtg ctgcaacgcg
      721 cgggcttcgg cgatcgacgc gcgatgatgg atcatcaggc tcgcgcccgc cgcgtgcaga
      781 cgccggcaaa tcgccgcgcc gacgcgcttg gcgccgcccg tcaccagcgc ggttttgccc
      841 gtgagtgtcc cagtggtttc catggcgcat attgtacggg aagccctgat ttgttatcat
      901 ttaatcagac cttcctctaa agtggcggaa accggggaca cgccgcttgc tgccagccca
      961 tttacagaaa cttcccgcgc cgtccgccga cgccctcgcc catagcgcgc ggctcaccgc
     1021 acggatcgcg cgcgaaatcg acgctgccgg cggctggttg agctttgcgc gctatatgga
     1081 gctcgcgttg catgcgccgg agctcggcta ttacagcgcg ggctcgacca agttcggtgc
     1141 ggccggcgat ttcgtcaccg cgccaacctt gggccgggta ttcggccgca cgctcgcgcg
     1201 ccaggctgcg caaatcctgc acgccggcat tcccgactta gtcgagctcg gcgccggcga
     1261 cggccggctc gcgcgtgatc tgctcgcgga actcgaagct ttgggcagct tgccgcagcg
     1321 ttacttaata ctagaaacca gcgcggactt gcgcgcgcgg cagcgtgacc tcctgcggcg
     1381 cgacgttcct cgcctcgccg gccgcgtcga atggttagac accctgcctg catcgctgac
     1441 ggcgctggtg ctcggcaacg aagtgctcga cgcgatgccc gtgcatgtca tcgaagttgg
     1501 cgccggcggc atcgctgagc gcggcgtcac gctcgcaggc ggcgaatttc aatggcaaac
     1561 gcgacccgcc accggcactg tgctagccgc cgcgcaggcg ctcgctctgc cgcccgggta
     1621 tatcaccgaa ataaatctgg ccgcgca
//
LOCUS       DATANS010000664         2035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_748655, whole genome shotgun sequence.
ACCESSION   DATANS010000664 DATANS010000000
VERSION     DATANS010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748655"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VHT22_09770"
     CDS             <1..198
                     /locus_tag="VHT22_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein LutB
                     domain-containing protein"
                     /protein_id="HEX3137886.1"
                     /translation="AGRPRLFAALTRIGVRMLHRMGGRDSLIHRLPLGGGWTDGRDMP
                     APAGRTFRELYAGRRTGGDKQ"
     gene            195..1424
                     /locus_tag="VHT22_09775"
     CDS             195..1424
                     /locus_tag="VHT22_09775"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX3137887.1"
                     /translation="MNKPRDAKAAPRISRRGESLGTENAFVVLAEVNALARQGRDIVS
                     FCIGQPDFPTPANIQDAAIAAIRAGKHGYTPSAGIDELRAAAARDLGQRRGLTIKPDD
                     VVIGAGAKPFIAYAIASVTDFGTEDEVIYPVPGFPIYESQIVANGALPVPIHLREARD
                     FAFDPQELEAKITAKTRLLILNSPHNPTGASLKTADLGVIAEILRRHPQIWVYADEIY
                     SRLCYDGEFASLASLPGMLERTIISDGASKTWAMTGWRLGYVANRALAPIFTRWITNT
                     DSCASQISQWAALEAITGPQGDAEAMRSSFLERRDLIVSLLNQVPGISCKTPGGAFYA
                     WPNVTGACKRTGCADSEEFRKRLLNEAGVAVLADIHFGRRVPGDGQHVRFSYAASPAA
                     IRSGVERIASFIKTSSR"
     gene            1421..>2035
                     /locus_tag="VHT22_09780"
     CDS             1421..>2035
                     /locus_tag="VHT22_09780"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEX3137888.1"
                     /translation="MSAAGDDPGRDRILGRVRDALAKSGVARDEAVARANAEAYVAAH
                     AQGPRPRMPDDLVARFMQRATDMESTVARIADRREIPASVARYLDALELAPALAAQRS
                     RQGVCWPELADLDWTGAGLAIEPRPTTGADKLGITGSFCAIAETGTLVLLSSALTPTG
                     TMLLPDTHVAIVSAARIVAGMEEAFALVRSERDPMPRAVNLVSGP"
BASE COUNT          345 a          701 c          667 g          322 t
ORIGIN      
        1 gcagggcggc cgcgcctctt cgccgcgttg acccgcatcg gcgtgcgcat gctgcaccgg
       61 atggggggcc gcgattcgct gatccatcgc ctgccgctcg gcggtggctg gaccgacggc
      121 cgcgacatgc cggcaccggc tggtcggacg tttcgagagc tctatgccgg ccggcgtacc
      181 ggcggagaca agcaatgaac aagccgcgcg acgccaaggc cgcgccaaga atcagccggc
      241 gcggagaaag cctcggcacc gagaatgcct ttgtggtgct cgccgaagtc aacgcgctgg
      301 cccgtcaggg ccgcgacatc gtctcgttct gcatcggcca gcccgacttt ccgacgccgg
      361 ccaatatcca ggatgccgcg atcgcggcaa tccgcgccgg caagcacggg tacacaccat
      421 cggcaggcat cgacgagctg cgcgcagccg cggcgcgcga ccttggccag aggcgcgggc
      481 tcaccatcaa gcccgacgac gtggtgatcg gcgccggggc caagcctttc atcgcctacg
      541 cgattgcgtc ggtgaccgat ttcggcaccg aggacgaggt gatttatccg gtgccgggct
      601 ttccgatcta cgaatcccag atcgtggcca acggtgcatt gccggtaccg atacacctgc
      661 gcgaagcgcg cgattttgcc ttcgatccgc aggagctcga agccaagatc acggcgaaga
      721 cccggctgct gatcctgaat tcgccgcata acccgaccgg cgcgagtctc aaaaccgccg
      781 atcttggcgt gatcgccgag atattgcgca ggcatccgca aatctgggtg tatgccgacg
      841 aaatctattc acgactgtgc tacgacggcg aattcgcctc gctcgcgtcg ctgcccggca
      901 tgctcgagcg cacgatcatt tcggacggag catcgaagac ctgggcgatg accggttggc
      961 gcctgggcta tgtcgccaat cgcgcgctgg ctccgatctt cacccgatgg atcaccaata
     1021 ccgattcctg tgcatcgcag atcagccagt gggcggcact cgaggccatc accggcccgc
     1081 agggcgacgc ggaggcgatg cgaagctctt tcctcgagcg gcgagacctg atcgtctctc
     1141 tgttgaacca ggttcccgga atcagctgca agacacccgg cggcgccttc tacgcctggc
     1201 cgaatgtgac cggggcatgc aagcggacag gttgcgccga ttcggaggaa ttccgcaagc
     1261 gcctgctgaa cgaagcgggt gtggcggtgc tcgccgatat ccatttcgga cgacgcgtgc
     1321 ccggcgacgg ccagcatgtg cgcttttcgt atgcggcatc gccggcggcg atccggtccg
     1381 gcgtcgagcg catcgcctcg ttcatcaaaa cgagcagccg atgagcgccg ccggcgacga
     1441 cccggggcga gacaggattc tcggtcgcgt gcgcgacgcg ctggcaaaat ccggcgtggc
     1501 gagggacgaa gccgtcgcgc gtgcgaacgc cgaagcgtat gtcgctgcgc acgcccaggg
     1561 cccacggccg cgcatgcccg atgatctggt cgcgcgtttc atgcagcgcg cgaccgacat
     1621 ggaaagcacc gtcgcccgca tcgccgatcg gcgcgaaatt ccggcgagcg tcgcccgcta
     1681 cctcgacgcg ctcgaactgg ccccggcgct ggccgcgcag cgctcgcggc agggcgtctg
     1741 ctggccggag ttggccgacc tcgactggac cggcgccgga ctcgcgatcg agccgcgccc
     1801 gaccacaggc gccgacaagc tgggcatcac cggctcattc tgcgcgatcg ccgaaaccgg
     1861 cacgctggtc cttctctcca gcgcactcac gccgaccggc acgatgctgc tgcccgacac
     1921 ccatgtcgcg atcgtgtccg ccgcgcgcat cgtcgccggc atggaggagg cgtttgcgct
     1981 ggtccgcagc gagcgcgatc cgatgccgcg cgccgtcaat ctcgtttccg gaccg
//
LOCUS       DATANS010000665         7649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_663334, whole genome shotgun sequence.
ACCESSION   DATANS010000665 DATANS010000000
VERSION     DATANS010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663334"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VHT22_09785"
     CDS             complement(<1..304)
                     /locus_tag="VHT22_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137889.1"
                     /translation="MMIGGGWQPTPAQEDLLEAAVGTSARAPEAWRSWRSRLGHLGPE
                     AIDGGSAQILPLLKPRLALLPADDPLLPLIVGCYRRSLYHGRMLRARAATLLALLAD"
     gene            complement(301..990)
                     /locus_tag="VHT22_09790"
     CDS             complement(301..990)
                     /locus_tag="VHT22_09790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX3137890.1"
                     /translation="MNAIDVVIPAFNAARYLGEALASVLSQDRAAHRIIVVDDGSTDG
                     TADVAAKFGAPVEITRRSNGGAAAARNAGIARSDAAVVAFLDADDRWIAHKLARQAAA
                     LDSDPTIDFVLSLVRAFASPELPEAERAALEAQQPRPFEGWVAGSMLIRRASFDRVGP
                     FGEDLHAGEVIDWFSRARRAGLNHLIVPEVLLERRLHRHNTTRLQAADRRGYLLAAKR
                     HVDRLRKDRER"
     gene            complement(987..1679)
                     /locus_tag="VHT22_09795"
     CDS             complement(987..1679)
                     /locus_tag="VHT22_09795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3137891.1"
                     /translation="MRLSVVLPVFNGASFLVAAIDSVLAQTRRADEIIVVDDGSTDDT
                     RAILERYAAVRVVNQANAGCAAARNAGAALASGDAIAFVDQDDIQCVDRFARQIDALQ
                     ADPKLGFVVCAQQNFLSETMVRPPPWCDPRTLAGPQHGFGANTLMLRREVLARVGPFD
                     PEKVPIDDSDWLLRALDGGERYLHLDTPLVRRRIHDANLSARRFAAGGTHLMARTLHE
                     SLQRRRGRGDTR"
     gene            complement(1679..2743)
                     /locus_tag="VHT22_09800"
     CDS             complement(1679..2743)
                     /locus_tag="VHT22_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137892.1"
                     /translation="MSAASAAAPELSLLAATLDKAVARLPAAPRLYRIAGDLVAIRAG
                     ERAAALLEAFTPMRASDDGDAVALEVFIFDAVSDGLALPDTAITHRRMAPTGGLGAFA
                     APGAQAFFQPDAGVLSLFDASRRRAYCWLRDSTALPYYEFAAPLRHLLQWWMLSRGGA
                     LLHSAAVGTRNGGVLIAGPSGSGKSTTALACQEAGLGFTSDDYVLVSCSGPPTVHMAY
                     STAKVVRESLGAHDVYRGHFRNLERGDEKPMMFMHEAAPQRVLRSFPLRAIVVPVIAQ
                     RSSTRIVAASAAEVLRALAPSSILLFPLAGATAFQRIAALCRAVPCLRAELSSDPHDV
                     AGAFARLLREETIAGVAAAA"
     gene            complement(2740..3201)
                     /locus_tag="VHT22_09805"
     CDS             complement(2740..3201)
                     /locus_tag="VHT22_09805"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX3137893.1"
                     /translation="MTDPQTAARYALNQPRVVGEVIDGEVMVINLDTGVYYSVTGAGA
                     AVWPMLVGGATRLEISDRVARHYGTDAASVDRDLGTFIARLADEAILRPRPDDAAAGL
                     SDAVEAWPAATYPGFGFERYDDMQALLVIDPVHEVGDFGWPSRSADDSEKA"
     gene            complement(3333..4289)
                     /locus_tag="VHT22_09810"
     CDS             complement(3333..4289)
                     /locus_tag="VHT22_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HEX3137894.1"
                     /translation="MRGSRSLACLALALAAGAAFAADVPYLTGRVVDNAEILAPATRD
                     KLAATLKAHEDASTDQIAVLTVPTIAGEAIEQYATRVFDAWKLGQKGKDNGVLVIVVP
                     NDRKMRIEVGYGLEGTLTDVASSRIIRDRMTPRFKEGNYDRGVSDGVDAIIAQLEGRA
                     ESAPASAPTASSSGAKSLVQIEGPELAWPMRILLGAFIFGIIGIFTVVGVMTPGVGWF
                     LYVFLIPFWAMFPIIIVGTRGALWLLGTYVVAYPIAKLILSRQPWYLKAASDLKSKGS
                     ASIGGFVMSSGSHSSGGFSSGGGGGFSGGGGSSGGGGSSGSW"
     gene            complement(4286..4948)
                     /locus_tag="VHT22_09815"
     CDS             complement(4286..4948)
                     /locus_tag="VHT22_09815"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX3137895.1"
                     /translation="MAHRAFLDHGESAALEQAIAALEAQTGAQLVTAVIGKSDSYVEL
                     PWKAFALGTALAGLALVVADALSPRWSGAENALIFAVAILGAGAASALLAVVAPPYAR
                     LFLRASRRDLEVRHYAQAFFLRRELFATRGRTGILLLVSLFERKVEILADVGLHARFD
                     AADWRTVIDAMTPLLRERRCFGALRQGVGRIEALLLAKGMTATRGSNELPDRPVQETG
                     AR"
     gene            complement(4961..5473)
                     /locus_tag="VHT22_09820"
     CDS             complement(4961..5473)
                     /locus_tag="VHT22_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3137896.1"
                     /translation="MNAALPPAGAATIREATTATDMALARALFAEYARWLKVDLCFQG
                     FDEELRTLPGAYAPPRGRLLLAGFGADAFACIALRPLAQPAVGEIKRLYVQPAQRGEG
                     WGRRLVAAVLAEARAIGYEELKLDTLDWMTAARALYEETGFLPCAPYYANPIPGVVYM
                     SLRMRGAVGA"
     gene            complement(5470..6120)
                     /locus_tag="VHT22_09825"
     CDS             complement(5470..6120)
                     /locus_tag="VHT22_09825"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX3137897.1"
                     /translation="MRTLVFLLLLANLSLFGYIKLDSLGTGEGVRLSQQIQPDKIKLL
                     TPQEVAALGPAKAASLADVCVEWGPLSDTDRNRALASLEPLDLSRLMTQKKVEVIASY
                     WVFLPPAASRAAADQRLAQLRASGIKDVSVIDSGPQRNAISLGVFRGEEAARNRLASL
                     QAQGLTNARLEPRTQSVQQTMLVVRDPTAQAMSRLKELQGEFPGSDTKIGSCDKQA"
     gene            complement(6227..7207)
                     /locus_tag="VHT22_09830"
     CDS             complement(6227..7207)
                     /locus_tag="VHT22_09830"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX3137898.1"
                     /translation="MLTPLGFRLLRRLADGEIHSGEALASAVGMSRARVSQVLKEADA
                     AGLKLERIRGRGYRLHDPLPFLDRDAIVAALGPVGKAVKVEVVDTVDSTSSELDRRSA
                     REDVHRRLLIAEWQTAGRGRRGRGWTAVAGGSLTFSLGWRFEQGAGFLAGLSLAVGVA
                     LARALEGVGIGGVELKWPNDLTHRFCKFGGILIELSGDALGPSLAVIGVGLNVRLAPA
                     ARRGIAQPVTDLATIAHGRIIDRNVLLAALMRELTAMLDRYAVDGFAPFIPEWQRRNA
                     FRGKSAKIILPDGSTASGDVVGVDASGALVLDHRGRRLRFVTGEVSLRRP"
     gene            complement(7267..>7649)
                     /locus_tag="VHT22_09835"
     CDS             complement(7267..>7649)
                     /locus_tag="VHT22_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX3137899.1"
                     /translation="EYLDLYHGEGIQHIALGTGDIYDTVHTLRQRGVAFQDTPDTYYE
                     ALATRLPGHQEDTARLKKERILLDGAPAKNEMLLQIFTTTVIGPIFFEIIQRKGNEGF
                     GEGNFRALFESIEQDQIRRGVLKG"
BASE COUNT         1320 a         2633 c         2508 g         1188 t
ORIGIN      
        1 cgtcggcaag gagcgcgagc aaggtcgccg ccctcgctcg cagcatccga ccgtgataga
       61 gggagcgccg gtaacagccg acgatcaacg gcagcagcgg gtcatcggcc ggaagcagcg
      121 caaggcgcgg tttgagcaac ggcagtatct gagcgctgcc gccgtcgatg gcttccggcc
      181 cgaggtgtcc caaccgtgag cgccacgatc gccatgcctc gggtgcccgc gctgacgtgc
      241 cgaccgcagc ttcgagcaga tcctcctgcg ctggcgtcgg ctgccagccg ccccctatca
      301 tcaccgttcg cggtccttgc gcaaccggtc gacatgccgt ttggccgcaa gcagataacc
      361 gcgacgatcg gccgcctgca aacgcgtcgt gttatgacga tgcaaacgcc gttcaagcag
      421 cacctcggga acgatcagat ggttgaggcc ggcccgccgc gcccggctga accaatcgat
      481 gacctcgccg gcatggaggt cttcgccgaa cgggccgaca cggtcgaacg acgcgcggcg
      541 aatcagcatc gatcccgcga cccagccctc gaacggacgc ggctgttgcg cctccagcgc
      601 ggcccgctcc gcttccggca gttccgggct cgcgaaggcg cggacaaggc tgagcacgaa
      661 atcgatggtc ggatcggagt ccaacgcggc cgcctggcgc gccagcttgt gcgcgatcca
      721 gcggtcgtcg gcatcgagga aggcgacgac ggccgcatcc gagcgcgcaa tacccgcatt
      781 gcgcgcggcg gccgcccccc cgttactgcg gcgcgtgatc tcgaccggtg cgccgaactt
      841 cgccgcgacg tcggcggtgc cgtcggtcga cccgtcgtcg accacgatga ttctatgcgc
      901 ggcgcgatcc tgcgacagca cgctcgccaa cgcttcaccc aggtaacgcg cggcattgaa
      961 tgccggaatg acgacgtcga tcgcgttcat cgcgtgtcgc cgcggccacg gcgccgttgc
     1021 agggactcat gcagcgtgcg cgccatcagg tgcgtgcccc ctgccgcgaa acgccgggcg
     1081 ctgagattgg catcgtgaat gcgtctccgc accagaggag tgtcgagatg aagatagcgc
     1141 tcgccaccgt cgagcgctcg aagcagccag tcggaatcgt ctattggcac cttttccggg
     1201 tcgaacggcc ccactctggc cagtacttcg cggcgcaaca tcaatgtgtt cgcgccaaat
     1261 ccgtgttgcg gccccgccag cgtacgcggg tcgcaccacg gcggcggacg gaccatcgtc
     1321 tcgctcagga aattctgctg cgcacagact acgaagccca gtttcggatc cgcttgcagc
     1381 gcgtcgatct gccgggcgaa gcgatcgacg cactggatgt catcctgatc gacgaacgca
     1441 atggcgtcgc cgcttgcgag cgcagcaccc gcattgcgcg ccgcggcgca gcccgcattg
     1501 gcctgattga cgacccgaac ggccgcgtag cgctccagta tcgcgcgggt gtcgtcggtc
     1561 gatccgtcgt cgaccacgat gatctcgtcc gcccgccggg tctgagcgag cacgctgtcg
     1621 atcgcggcga ccagaaagga ggcgccgttg aacaccggca gaacgacgct gagccgcatc
     1681 acgcggccgc cgccacgccg gcaattgtct cctcgcgcag caatcgcgca aatgcgccgg
     1741 cgacatcgtg cggatcgctg gaaagctccg cgcgcaagca cggcactgcc cggcacagcg
     1801 ccgcgattcg ctggaacgcg gtcgccccgg ccagcggaaa cagcaggatc gagcttggcg
     1861 caagtgcgcg caggacctcg gctgcgctcg ccgcgacaat ccgcgtcgag ctgcgctgcg
     1921 caatgacggg caccacgatc gcccgaagcg gaaaggaccg caggacgcgc tgcggcgcag
     1981 cctcgtgcat gaacatcatc ggtttttcgt cgccgcgctc gagattacga aagtggccac
     2041 ggtagacgtc gtgcgcaccc aggctttcgc gcacgacctt cgccgtcgaa taagccatgt
     2101 gcacggtcgg cggtccgctg caggagacca gcacatagtc gtcgctggta aatcccaacc
     2161 cggcttcctg gcaggccagc gcggtcgtgg atttgccgct gccgctggga cccgcgatca
     2221 gtaccccgcc gttgcgcgtg cccacggcgg cgctatgcag cagcgccccg ccgcgcgaaa
     2281 gcatccacca ctgcagcagg tgccgcaacg gggccgcgaa ttcatagtac ggcaacgcag
     2341 tcgaatcgcg cagccagcag taagcgcgtc ggcgcgatgc gtcgaacaac gaaaggacgc
     2401 cggcgtcggg ttgaaaaaac gcctgagctc cgggcgcggc gaacgcgccg agcccgcctg
     2461 tcggcgccat ccggcgatgc gttatggcgg tgtcgggaag cgccaacccg tcgctcaccg
     2521 catcgaagat aaagacctcg agcgcgacgg cgtccccgtc gtcgctcgcc cgcatcgggg
     2581 tgaaagcctc gagcagcgcg gcggcccgct cgccggcgcg aatcgcaacc aggtcgccgg
     2641 cgatgcgata caggcgtggg gcggcaggca gccgcgcgac ggccttgtcg agcgtcgcgg
     2701 cgagaagcga cagctccggc gccgcggccg acgcggcgct catgccttct cgctgtcgtc
     2761 cgcgctgcgc gacggccaac cgaaatcgcc gacctcgtgc accggatcga tgaccagcag
     2821 cgcctgcatg tcgtcgtaac gctcgaagcc gaatccggga tacgtcgccg ccggccatgc
     2881 ctcgaccgca tcggacaacc cggccgcggc gtcatccggc ctcgggcgca gtatcgcctc
     2941 gtcggccagc cgcgcgataa acgtgccgag atcgcggtcg accgacgcgg cgtcggtgcc
     3001 gtagtgccgc gccactcggt cgctgatttc cagtcgcgtt gctccgccga ccagcatcgg
     3061 ccacacggcg gcgccggcac cggtgacgct gtaatagacg ccggtgtcga gattgatgac
     3121 catgacctcg ccgtcgatga cttcaccgac gactctgggc tggttcagtg catagcgtgc
     3181 agcggtttgc gggtcggtca tggcggggaa gggaacttgc cgggccaatg atatccggag
     3241 gcggcgccga tgcaaattcc cccggcctcc ccgcaatcgt cgccgttcgc cgcgacgggg
     3301 gccaacggcg cggcgaccca cataagaccg atttaccagc tcccggagct gccgccgccg
     3361 ccggaactac cgccgccacc cgagaatccg ccaccaccgc cggaggagaa tccccccgaa
     3421 gagtggcttc ccgagctcat cacgaatccg ccgatgctgg cgctgccttt gctcttgaga
     3481 tcgctcgccg ccttcagata ccacggctgc cgagacagga tcagcttggc gatcggatag
     3541 gcgacgacgt aggtgccaag cagccacagt gcgccgcgcg ttccgacgat gatgatcgga
     3601 aacatcgccc agaacggaat caggaacacg tacaggaacc agccgacgcc cggcgtcatc
     3661 acgccgacga cggtgaagat tccgatgatg ccgaaaatga acgcgccgag taggatgcgc
     3721 atcggccacg cgagctccgg accttcgatc tgcaccagcg acttggcgcc gctcgagctc
     3781 gccgtcggcg cgcttgccgg cgccgattcc gcgcggccct cgagctgggc gatgatcgcg
     3841 tcgacgccgt cgcttacgcc gcggtcgtaa ttgccctcct tgaagcgagg cgtcatccgg
     3901 tcgcgaatga tgcggctgga tgcgacatcg gtcagcgtgc cttcgagccc gtagccgact
     3961 tcgatgcgca ttttccggtc gttcggcacg acgatgacca gcacgccgtt gtccttgccc
     4021 ttttgtccga gcttccaggc atcgaacacg cgggttgcgt attgctcgat cgcctcgccg
     4081 gctatcgtcg gtaccgtcag caccgcgatc tggtcggtgc tggcgtcttc gtgtgccttt
     4141 agcgtggcgg caagcttgtc gcgcgtcgcc ggggcgagga tctcggcgtt gtcgacgacg
     4201 cgtccggtca gatacggcac gtcggcggcg aacgcggctc ccgccgcgag cgcaagcgca
     4261 agacacgcga gcgatcgtga gcccctcatc gcgctccggt ctcctgaaca ggccggtcgg
     4321 ggagctcgtt gctgccccga gtcgcggtca ttcccttggc aagcagcagc gcctcgatgc
     4381 gcccgacgcc ctgccgcaag gcaccgaagc agcggcgttc gcgcaacaaa ggcgtcatcg
     4441 cgtcgatcac cgtccgccag tcggccgcat cgaagcgcgc gtgcagcccg acgtcagcca
     4501 gaatttcgac cttgcgctcg aacaggctca ccagcagcag aatgcccgtg cgcccgcggg
     4561 tggcgaacaa ttcgcggcgc aggaaaaacg cctgcgcata atgacgaact tccagatcgc
     4621 ggcggctcgc gcgcaggaac agccgcgcgt aaggcggcgc gacgacggca agcagcgcac
     4681 tggcagcacc cgcacccagg atcgcaaccg cgaagatcag cgcattctcg gcaccgctcc
     4741 agcgcggcga cagcgcatca gcgaccacga gcgcgaggcc ggcaagcgcc gttccgagcg
     4801 cgaatgcctt ccagggcaac tcgacgtagc tatcggactt gccgatgact gcggtgacga
     4861 gctgcgcgcc ggtttgcgcc tccaaagcgg cgatcgcctg ttctagggcg gccgattcac
     4921 cgtggtcgag aaacgcccgg tgtgccacgg aatcgccgcg tcaagcgcca acggcgccgc
     4981 gcatccgcag cgacatgtag acgacgccgg gaatcggatt ggcgtagtac ggcgcgcaag
     5041 gcagaaatcc ggtctcttca tacagtgcgc gcgcggcggt catccagtcc agcgtatcga
     5101 gcttgagctc ttcgtagccg atcgcgcggg cctcggcgag caccgcggcg acgagcctcc
     5161 tgccccagcc ctcgccgcgt tgcgccggct gcacatacag ccgcttgatt tcgccgaccg
     5221 cgggttgagc cagagggcgt aatgcgatgc acgcgaacgc gtcggcaccg aatccggcaa
     5281 gcaacagccg cccgcgcggc ggcgcgtagg cgccgggcag cgtgcgcaat tcctcatcga
     5341 agccctggaa gcacagatcg accttcagcc agcgcgcgta ttcggcgaac agagcgcgcg
     5401 cgagcgccat gtccgtggcg gtcgtcgcct cgcgaatcgt cgcggcgccc gccggaggca
     5461 gcgcggcgtt caagcctgct tgtcgcacga gccgatcttg gtgtcgctgc ccgggaactc
     5521 gccctgcagt tctttcaggc gcgacatcgc ctgcgccgtc ggatcgcgca ccaccagcat
     5581 cgtctgctgc accgactgcg tacgaggctc gagcctcgcg ttggtgagcc cttgcgcctg
     5641 cagcgacgcg agtcgattgc gggcagcttc ttcgccgcgg aacactccca gcgagatcgc
     5701 attgcgttgc ggtccgctgt cgatgaccga gacgtccttg atgccgctgg ctctgagctg
     5761 ggcgaggcgc tggtcggccg cggcgcgact ggcggccggc ggcagaaata cccagtaact
     5821 ggcgatgacc tcgacttttt tctgcgtcat caggcgagaa agatccagtg gttcgagact
     5881 cgccagcgcg cgattgcggt cggtatccga cagcggaccc cactccacgc atacgtcggc
     5941 cagcgacgcc gccttggccg gtcccagcgc cgccacctcc tgcggcgtca gcagcttgat
     6001 cttgtcgggc tggatctgct gcgacaaacg aacgccctcg ccggtgccga ggctgtcgag
     6061 cttgatgtag ccgaacaggc tcaggttggc gagcagaaga aggaagacca gcgttctcat
     6121 cagggcctgt tcacgttaaa tgcattgcca ggctggaagc cggcgcgatg cggcgattat
     6181 ccacgaccgg caccgcggcc ggaagcggag ctttggcgct ggccgttcat ggccggcgca
     6241 gcgaaacctc gccggtgaca aagcgcagcc tccggccgcg atggtcgagg accagggcgc
     6301 cgctggcgtc gacaccgacg acgtcgcccg acgccgtgct gccgtcgggc aggatgattt
     6361 tcgccgactt gccgcggaat gcattgcgtc gctgccattc ggggatgaac ggggcgaagc
     6421 cgtcgacagc gtagcgatcc agcatcgccg tcaattcgcg catcagcgcg gcgagcagga
     6481 cgttgcgatc gatgattcgt ccgtgcgcga ttgtcgcaag atcggtgacc ggctgcgcta
     6541 tgccgcgccg cgccgcgggc gccagccgca cattgagtcc gacgccgatt accgcaagcg
     6601 acggtccgag cgcatcgccg gagagctcta tcaggatgcc gccgaacttg cagaaacgat
     6661 gagtgaggtc gttcggccat ttgagctcga cgccgccgat gcccacgcct tccagtgcgc
     6721 gcgcgagcgc cacgccgacg gcgagcgaga ggcccgcaag gaagcccgcc ccctgctcga
     6781 atcgccagcc gagcgaaaag gtgaggctgc cgccggctac cgcggtccag ccccggccac
     6841 ggcggccgcg acccgcggtc tgccattcgg cgatcagcag ccggcggtgc acgtcttccc
     6901 gcgcggagcg gcggtcgagc tcgctgctag tcgaatcgac ggtgtcgacg acttccactt
     6961 tcaccgcttt tcccaccgga ccgagcgccg ccacgatggc gtcgcgatcg agaaagggaa
     7021 gcggatcatg caggcgatag ccacgcccgc ggatgcgttc cagcttgagt ccggcggcgt
     7081 ccgcttcctt cagcacctgc gagacgcgcg cacgcgacat cccgaccgcg ctcgccagcg
     7141 cttcaccgga gtggatttcg ccgtcggcaa gacggcgcag caagcggaaa ccgagaggcg
     7201 tcagcacgtg tgttgtcaga gcgtttatcg aaaccggcgc accgcgcgcc gcggccttca
     7261 gccccttcag ccctttagca cgccgcggcg aatctgatcc tgctcgatcg actcgaacag
     7321 tgcacggaaa ttgccttcgc cgaagccctc gttgcccttg cgctggatga tttcgaagaa
     7381 gatcggtccg atgacggtcg tggtgaagat ctgcagcagc atttcatttt ttgcgggagc
     7441 gccgtcgagc aggatgcgct ctttcttcag gcgggcggtg tcttcctggt gcccgggaag
     7501 gcgcgtggca agcgcctcgt agtaggtgtc cggcgtgtcc tgaaacgcca cgccgcgctg
     7561 gcgcagcgta tgcacggtgt catagatgtc gccggtgccg agcgcgatgt gctggatgcc
     7621 ctcgccgtgg tacaggtcca ggtattcgg
//
LOCUS       DATANS010000666         1627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_663340, whole genome shotgun sequence.
ACCESSION   DATANS010000666 DATANS010000000
VERSION     DATANS010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663340"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..851)
                     /locus_tag="VHT22_09840"
     CDS             complement(51..851)
                     /locus_tag="VHT22_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HEX3137900.1"
                     /translation="MTVILEAQSVSLAIGGATLVDGVDLRVKAGEMVAIVGPNGAGKS
                     TLLRMLSGDLRPTKGRIELRQRDIHLYAPRVLAHHRAMLSQHVNVTFPFTVEEIVHMG
                     AGDTGRAAAQRLVDAALGEVGLTPFSHRQLPTLSGGEQQRAHFARVLVQLACGEQLHG
                     PGLLLLDEPTSSLDMRHQIDLVETARRRAQNGTAVIAVLHDLNLAMRFADRIVLLHRG
                     KLAVDGGRAEAITAETIRRIFEVDVTIDYTDQGVPFLLPQTMRPAGPP"
     gene            complement(848..>1627)
                     /locus_tag="VHT22_09845"
     CDS             complement(848..>1627)
                     /locus_tag="VHT22_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEX3137901.1"
                     /translation="RNPLADPALVGVSSGGALAAAAAIVFTDSQIGQSFRFMQHQLLP
                     IAAFAGSLVTTVVLYSIASRSGRTSIAIFLLAGIAIAAIANAGIGLLVFIADDRQLRD
                     ITFWLLGSLSGATWPKLATLAPVLGLALIACVSIARGLDVLVLGEAEAFHSGVDVERL
                     KRISIVLVSAMTGVAVSVCGVVGFVGIVVPHLLRLVIGPAHRLLLPASMLLGAVLMVG
                     ADTLARTIVAPAEMPIGILTAAIGAPFFLGMLLRQRGLVSL"
BASE COUNT          309 a          548 c          553 g          217 t
ORIGIN      
        1 cgcgtcaaaa caaaaacctg gagccggttc tgattcaatc agaaccggct ctagggcggg
       61 ccggctggcc gcatcgtctg cggcaacagg aacggcacgc cctgatcggt atagtcgatc
      121 gtgacgtcga cctcgaaaat ccggcggatg gtctcggccg tgatcgcctc cgcacggccg
      181 ccgtcgacgg cgagcttgcc gcgatgcagc agcacgatcc ggtcggcgaa ccgcatcgcc
      241 agattgaggt cgtgcagcac ggcgatcacg gcggtgccgt tctgcgcgcg ccgccgcgct
      301 gtctccacca ggtcgatctg gtggcgcatg tcgaggctgg aggtcggctc gtccagcaac
      361 agcaggccgg ggccgtgcag ttgttcgccg caggcgagct gcaccagcac gcgggcaaaa
      421 tgggcgcgct gctgctcgcc gccggagagc gtcggcaatt gccggtggct gaaaggcgta
      481 agcccgacct cgcccagcgc cgcatcgacc agccgctgcg cggcggcgcg gccggtgtcg
      541 cccgcgccca tatggacgat ttcctcgacc gtgaacggga aggtgacgtt gacgtgctgc
      601 gacagcatcg cacggtgatg ggcgagaacg cggggcgcat agaggtggat gtcgcgttgc
      661 ctcagctcga tccggccctt ggtcgggcgg agatcgccgg acagcatccg cagcagcgtc
      721 gatttgccgg cgccgttggg gccgacgatg gcgaccatct cgccagcctt gacgcgcaga
      781 tcgacgccgt cgaccagcgt ggcgccaccg atcgccaacg agaccgactg cgcttcgaga
      841 atgacggtca tagcgaaacc agcccgcgct gccgcaacag catgcctaga aagaacggtg
      901 cgccgatcgc cgccgtcagg atgccgatcg gcatttccgc gggcgccacg atggtgcggg
      961 ccagcgtatc ggcgcccacc atcagcaccg cacccagcag catcgaggcc ggcagcagca
     1021 gccggtgcgc cggcccgatc accagccgga gcagatgcgg caccacgatg ccgacaaagc
     1081 cgaccacgcc gcagaccgaa accgcaacgc ccgtcatcgc cgacaccagc acgatcgaaa
     1141 tccgcttcag ccgctcgacg tcgacgccgc tgtgaaacgc ctcggcctcg cccaatacca
     1201 gcacatcgag cccgcgcgcg atcgagacgc aggcgatcaa ggcgagcccc agcacgggtg
     1261 ccagcgtggc gagcttgggc caggtcgcgc cgctcagcga gcccagcagc cagaacgtga
     1321 tatcgcgcaa ctggcggtcg tcggcgatga agacgaggag cccgatgccg gcattggcga
     1381 tggcggcaat tgcaatgccg gccagcagaa aaatcgcgat cgacgtccgc cccgagcggc
     1441 tggcgatcga atagaggacg accgtcgtga ccagcgagcc cgcaaacgct gcgatcggca
     1501 gcaattgatg ctgcatgaag cggaagcttt ggccgatctg gctgtcggtg aatacgatcg
     1561 ccgccgccgc tgcgagcgcg ccgccggagg aaacgccgac caatgcgggg tcggcgaggg
     1621 ggttgcg
//
LOCUS       DATANS010000667         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_240769, whole genome shotgun sequence.
ACCESSION   DATANS010000667 DATANS010000000
VERSION     DATANS010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240769"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..512)
                     /gene="arfB"
                     /locus_tag="VHT22_09850"
     CDS             complement(111..512)
                     /gene="arfB"
                     /locus_tag="VHT22_09850"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX3137902.1"
                     /translation="MSVRIAPDEVELTAVRAQGAGGQNVNKVSNAVHLRFDVRGSTLP
                     ETVKARLLAHSDRRITAEGVVIIKAQRYRSLDQNRADALARLQRLVDGAAIAPRPRKA
                     TKPTAGSQRRRVDSKVRRGEVKAARRRIVDD"
     gene            complement(509..1135)
                     /locus_tag="VHT22_09855"
     CDS             complement(509..1135)
                     /locus_tag="VHT22_09855"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013210494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3137903.1"
                     /translation="MTLAADELAAIAARTVEHYEQRAESFWEGTRDHDVSQNIATLLK
                     HIEGEPPLAILDLGCGPGRDLKTLRALGHAPVGLEGSPRFAAMAREHSGCEVWEQDFL
                     RLDLPARRFDGVFANAALFHVPTQELPRILREIRATLKPRGVLFSSNPHGDDSEGWNR
                     GRYGAYLRHETWRDYLAGAGLVEIEHYYRPAGMPREQQPWLATVARAP"
     gene            complement(1160..2146)
                     /locus_tag="VHT22_09860"
     CDS             complement(1160..2146)
                     /locus_tag="VHT22_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3137904.1"
                     /translation="MIRPPTASGRADAEAGRSTLAPVPTDARVEGVEHWAHKGPVRLF
                     LWEKFVDSPQDKPLLLFVHGSSMASQPTFDLAVPGRPDSSVMDWFARRGFVCWCVDME
                     GYGRSDKWRDTNADIANGADDLDAATEYILKTRSVGRSLIYGISSGALRAALFAQRHP
                     GRVERLALDAFVWTGAGAPTLEQRRKKLPEFLAARRRPIDRAFVYSIFARDHPDTADK
                     RVVDAFADAILALDDSMPNGTYIDMCSKLPVVDPALLTMPTLIMRGQYDGIAALDDLI
                     EFFKRLPHPDKQFSIMAGISHASFQQKNYLIAYHILHAYFTQPEPQYVGPAD"
BASE COUNT          368 a          737 c          752 g          393 t
ORIGIN      
        1 tgggcgcgcg cggcgccgcg catgttcatg ccggtttcac ctggaccaca gtgaccgacc
       61 gcctggtctc ctgcctcttc gccggcgcgt gaatgcgttg cctgggcgtc tcagtcgtcg
      121 acgatgcggc ggcgcgccgc cttgacctcg ccgcgacgca ctttgctatc gacccgccgt
      181 cgctgcgagc ccgcggtcgg cttggtggcc ttgcgcggcc gcggcgcaat ggccgccccg
      241 tcgaccagcc gctgcagccg agccagagca tcggcgcgat tctggtcgag gctgcgataa
      301 cgctgcgcct tgatgatgac gacgccttcc gcggtgatcc gacggtcgct gtgcgcgagc
      361 aggcgggcct tgacggtttc gggcagcgtc gagccccgca cgtcgaagcg cagatgcacg
      421 gcgttcgaaa ccttgttgac gttctgcccg ccggcgcctt gtgcgcgcac ggccgtcagc
      481 tcgacttcat cgggcgcgat gcgcacgctc acggcgctcg ggcaacggtg gccagccagg
      541 gctgctgctc gcgcggcatg ccggcgggac ggtaatagtg ctctatttcg accagtccgg
      601 cgccggcaag atagtcgcgc cacgtttcat ggcgaaggta ggcaccgtag cggccgcggt
      661 tccaaccttc gctgtcgtcg ccgtgcggat tcgagctgaa caatacgccg cgaggtttga
      721 gcgtcgcacg aatctcccga aggatgcgcg gcaattcctg cgtcggcacg tgaaacagcg
      781 cggcattggc gaagacgccg tcgaagcggc gggctggcag gtcgaggcgg aggaagtcct
      841 gctcccagac ctcgcacccg ctgtgctcac gcgccatcgc cgcgaaccgc ggcgatccct
      901 cgagcccgac cggtgcatga ccgagcgcgc gcaaggtctt cagatcgcgc ccgggaccgc
      961 agcccaggtc gaggatcgcc aagggaggct cgccttcgat gtgcttcaaa agcgtcgcga
     1021 tgttctggct gacgtcgtga tcgcgcgtcc cttcccagaa actttccgca cgctgctcgt
     1081 agtgctcgac cgtgcgcgcc gcgatcgccg ccagctcgtc cgcagcgagc gtcatcgggc
     1141 acgccgcggg cgcaacgggt caatcggccg gtccgacata ttggggctcc ggctgagtga
     1201 aataagcgtg caggatgtga taggcgatca ggtaattctt ttgctggaag ctcgcatgcg
     1261 atatgccggc catgatcgaa aactgcttgt ccggatgcgg caggcgcttg aagaattcga
     1321 tcagatcgtc gagcgcggcg ataccgtcgt actggccgcg cataatcagc gtcggcatcg
     1381 tcagcagggc cggatcgacg accggaagct tcgagcacat gtcgatgtag gttccgttcg
     1441 gcatcgagtc gtcgagcgcg agaatcgcgt cggcgaaggc atcgacgacg cgcttgtcgg
     1501 cagtgtccgg atgatcgcgc gcgaaaatgg aatagacgaa cgcgcgatcg atcggccggc
     1561 gcctggcggc aaggaattcc ggcagcttct tccgtcgctg ctcgagtgtc ggcgcaccgg
     1621 cgccagtcca gacgaaagca tccagcgcga gtcgctcgac ccttcccgga tgtcgctggg
     1681 cgaacaatgc cgcgcgcagc gcgcccgacg aaatcccgta gatcagcgac cggcctacgc
     1741 tgcgcgtctt caggatgtat tcggtcgcgg cgtcgagatc gtcggcgccg ttggcgatgt
     1801 cggcgttggt gtcgcgccac ttgtccgagc ggccatagcc ttccatatcg acgcaccagc
     1861 agacgaagcc gcggcgcgcg aaccaatcca tcaccgacga gtcgggcctg ccgggaacgg
     1921 caaggtcgaa cgtcggttgc gacgccatcg acgagccgtg aacgaacagc agcagcggct
     1981 tgtcttgtgg cgagtcgacg aatttttccc agagaaacag gcggaccggc cccttgtgcg
     2041 cccagtgctc gacgccttca acgcgcgcgt cggtcggcac aggcgccaag gtcgagcgtc
     2101 ccgcttccgc gtcggcgcgg ccgcttgcgg tgggtggtct aatcaatttt cgctccggtc
     2161 tcgcgaatga tctttgccca ttggtccttc tcggcacgga aaaacgccgc gaactcggcg
     2221 ggcgtcgacc cgaccggctc gatgctggcc
//
LOCUS       DATANS010000668         1767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_285734, whole genome shotgun sequence.
ACCESSION   DATANS010000668 DATANS010000000
VERSION     DATANS010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285734"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VHT22_09865"
     CDS             <1..467
                     /locus_tag="VHT22_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX3137905.1"
                     /translation="AAASPQQLRDFAIEHLVPHKVPSVVVLVPDLPRNALGKVWRRGL
                     ADTLRTALQSAYVPPRDDDQRMIAAIFAEVLGLPRVGALDNFFAIGGDSLRAAQVLSR
                     VEARTGAQMEMLSLFKAPTVDRLAERMRAALREGPRLDQPPLVTPRHRRPSA"
     gene            464..1519
                     /locus_tag="VHT22_09870"
     CDS             464..1519
                     /locus_tag="VHT22_09870"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX3137906.1"
                     /translation="MSTTTVPSQQDDRRRAAKRSCTRVVALLAFVVAPMSVSHFALAQ
                     DYPVKPVRIVVGQSAGSAADAVARLIADSLSGVWQQPVNVENKGGAAGTIAADLVAKS
                     PADGYALLLGGQGNLVIAATLDPALRYDPSRDFAPIARLASTPFFLITSAGVPAKTLP
                     ELVAYAKANPGKLTYISYGDGSVSRVAFQWLETAAGIDLLEIPYKGSAPATADLLAGR
                     VDMGLYDFSAVEQHVSAGTLRMLAAIGATRATSAPDVATVAEQGVPGYAIEAWYGLLA
                     PAGTPPDVVARISGAIDEIRRTASFRRRLADLSYEPVIDTPVQFAAVIRSDIARYAEI
                     IKRAGIDARRNPRGATQ"
     gene            1540..>1767
                     /locus_tag="VHT22_09875"
     CDS             1540..>1767
                     /locus_tag="VHT22_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137907.1"
                     /translation="MITDPDPMPETSADRNDHRAAAGDSYSEIMSFGQQRLWVLDKLL
                     PDRSVYNTPTVQRLIGRLDVAALETSIGEILN"
BASE COUNT          284 a          596 c          579 g          308 t
ORIGIN      
        1 ctgcggcggc gagcccgcag caattgcgcg attttgcgat cgagcacctc gtgccgcaca
       61 aggtgcccag cgtcgtcgtg ctggtgcctg atctgccgcg taacgcgttg ggcaaagtct
      121 ggcggcgtgg ccttgcggat acgctgcgca ccgcgctgca aagtgcgtat gtcccgccgc
      181 gcgacgacga ccagcggatg atcgctgcaa ttttcgccga ggtgctcggc ctgccgcgcg
      241 tcggtgcgct cgataatttt ttcgccatcg gcggcgattc gctgcgtgcg gcgcaagtgc
      301 tctcacgcgt cgaagcccgg accggggcgc agatggagat gctgtcgctg ttcaaggcgc
      361 cgacagtcga tcgattggcc gagcggatgc gcgctgccct gcgtgaaggg ccccgcctcg
      421 atcagccgcc tcttgtaacg ccgcgtcacc gccgtccctc ggcatgagca ccaccaccgt
      481 tccgtcgcag caagacgacc gccggcgcgc ggcgaagcga tcgtgcaccc gcgtcgtcgc
      541 gctgctggcg ttcgtcgtcg ctccgatgtc ggtgtctcac ttcgctctgg cacaggacta
      601 ccccgtcaag ccggtcagga tcgtcgtcgg ccagagcgcc gggagcgcag ccgatgcggt
      661 ggcgcgtctg atcgcagact cgttgtcggg cgtgtggcag caaccggtca acgtcgagaa
      721 caagggtggg gccgcgggca cgatcgccgc cgacctggtt gcgaagtcgc ccgccgatgg
      781 atacgcattg ctcctcggcg gccagggcaa tctcgtcatc gcggcgacgc tcgacccggc
      841 gttgcgctac gatccgtcgc gcgatttcgc gccgatcgct cgccttgcga gcacgccgtt
      901 cttcctgatt accagcgccg gcgttcccgc gaagacgcta cccgaactgg tcgcgtacgc
      961 caaggcgaat cccggcaagc tcacctatat ctcatacggt gatggatcgg tcagccgggt
     1021 cgccttccaa tggctggaga ctgccgccgg catcgacctg ctggagatac cctacaaagg
     1081 ctcggcgcct gcgaccgccg acctgctggc cggcagggtc gacatgggcc tctacgactt
     1141 ttcggcggtc gagcaacatg tgagcgctgg cacgttgcgc atgcttgccg ccatcggcgc
     1201 gacgcgcgcc acttcggcgc ccgacgtggc gaccgtggcc gagcaaggcg tgcccggcta
     1261 tgcgatcgaa gcctggtatg ggctcctcgc accggccggc acgccgcccg atgtcgtagc
     1321 caggatcagc ggcgcgatcg atgaaattcg ccgcaccgcc tctttccggc gacgcctcgc
     1381 cgatttgagt tacgaaccgg tcatcgatac gccggtccag ttcgccgcgg tcatccgctc
     1441 ggatatcgcc aggtatgccg agatcatcaa gcgggccggg atcgatgcgc gacgcaaccc
     1501 gcgaggcgcg acgcaatgag cgagcgccca tcgcgagccg tgatcaccga tcccgatccc
     1561 atgccggaga cgagcgccga tcgcaacgat catcgagccg cagcgggtga ttcgtactcg
     1621 gagatcatgt ccttcgggca gcagcgtctg tgggttctgg acaagctgct gcccgaccgg
     1681 tcggtgtaca acacgccaac ggtccaacga ctgatcggtc ggctggacgt ggcggcgctg
     1741 gaaaccagta ttggcgaaat cctgaat
//
LOCUS       DATANS010000669         2663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_159771, whole genome shotgun sequence.
ACCESSION   DATANS010000669 DATANS010000000
VERSION     DATANS010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159771"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="VHT22_09880"
     CDS             <1..1204
                     /locus_tag="VHT22_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HEX3137908.1"
                     /translation="GARLAHDLARVCQAQADLFGGAPGSRAPVDAYLFQLLAVHDGYG
                     GLEHRASTSLIANRDGLPQPGATGVSDDYRSLLGLASHEYFHTWNVKRIKPAAFVPYD
                     LTRENFTTQLWAFEGITSYYDDLMLVRSKVIGVGDYLELLGRQISALLRTPGRERQSV
                     AESSFDAWIKYYRQDENAPNAIVSYYVKGALIALVLDLELRLHSAVTLDDVMRALWKR
                     HGQTGLGVPEDGVQVIAAELSGLDLGAFFRDYVHGTVELPLPSLLREFGIGWNLRPSA
                     NAKDKGGAPGKDPAPRVWLGATLAAGTDLRLKHLSPEGPAALAGLASGDAIVAIDSLR
                     ASADGLERLAQTRNAGDVVEIHAFRSDELMRFSIELAPARNDTCWLTLAEAVGPEAAA
                     RRTAWLGA"
     gene            complement(1215..1865)
                     /locus_tag="VHT22_09885"
     CDS             complement(1215..1865)
                     /locus_tag="VHT22_09885"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX3137909.1"
                     /translation="MAITLYYGSGSPYAWRAQLALEHKALPYELKVLSFAAGDTRKPE
                     FLALNPRHRVPVLVEDDFVLYESSAIVEYLDEAYPGRGAPLFPGDARTRAIIRRMICE
                     VDEDFDAALDPLTTQAFSKKPEERDAKAIADSRKALVDELALVSKSMRGDYLAGPLSA
                     ADYALYTLLAFVWRSQIKLPDLDADGMLTPQLARWKARIEALPYFDKTIPPHWRKS"
     gene            complement(1881..2231)
                     /locus_tag="VHT22_09890"
     CDS             complement(1881..2231)
                     /locus_tag="VHT22_09890"
                     /inference="COORDINATES: protein motif:HMM:NF043076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HEX3137910.1"
                     /translation="MPDQPSMPLTPQEAMEFMQRMWNPFGVPVPGFSPPGAPGAAAAT
                     IPFPNPAAMIATLDPAEIDKKIAELRVIENWLGMSQNFLQMSIKTMELQKASLEAMRA
                     ATAPAKPGKKKSGS"
     gene            2292..>2663
                     /locus_tag="VHT22_09895"
     CDS             2292..>2663
                     /locus_tag="VHT22_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3137911.1"
                     /translation="MFDAAASLLVTLKSIVGEANVLTADADVASYVTDWRERYRGRAR
                     AVVRPGSTDEVAAVVRCCAGHRVPVVPQGGNTGLCGGATPHATGDEVVLTLTRMNRVR
                     AIDADDATMTVEAGMPLASVQQ"
BASE COUNT          442 a          868 c          887 g          466 t
ORIGIN      
        1 cggcgcgcgg ctggcgcacg atctggcgcg cgtgtgccag gcgcaggccg acctgttcgg
       61 cggcgcaccg gggagccgtg cgccggtcga tgcctatctg ttccagctgc tggcggtgca
      121 cgacggctat ggcgggctcg agcatcgcgc gagtacgagc ctgattgcca accgggatgg
      181 cctgccgcaa ccgggtgcga ccggcgtcag cgacgactac cggtcgctgc tgggcctcgc
      241 gagccacgag tacttccata cctggaacgt caagcgcatc aagccagcgg ccttcgtgcc
      301 gtacgacctg acccgtgaga atttcacgac gcagctctgg gccttcgagg ggatcacgtc
      361 ctactacgac gatctgatgc tggtgcgcag caaggtcatc ggcgtcggcg actatctgga
      421 actgctcggc cgtcagatca gcgcgctgct gcgcacgccg gggcgggaaa ggcaaagcgt
      481 cgccgaatcg agtttcgatg cgtggatcaa atattatcgg caggacgaga acgcgccgaa
      541 tgcgatcgtc agctactacg tcaagggcgc gttgatcgcg ctcgttttgg atctcgagct
      601 gcggctgcac agcgcggtca cgctcgacga cgtcatgcga gccctatgga agcgtcatgg
      661 ccagaccggc ctcggcgtgc ccgaagacgg cgtccaggtg atcgcggccg agctttccgg
      721 gctcgatctt ggtgcgttct tccgcgacta tgtccacggc acggtcgagc tcccgctgcc
      781 gtcgctgctg agggaattcg gcatcggctg gaacctgcgg cccagcgcga acgcgaagga
      841 caagggtggc gcgccaggca aagacccggc gccgcgcgtc tggctgggtg cgaccctggc
      901 tgccggcacg gacctgcggc tcaagcatct aagcccggag ggcccggcag cgctcgccgg
      961 cctcgcatcg ggcgacgcga tcgttgccat cgacagcctg cgggcgtccg ccgacggact
     1021 cgagcgcctt gcccagacga ggaacgccgg cgacgtcgtc gagattcacg cattccggag
     1081 cgacgagctg atgcgcttct cgatcgagct cgcccctgcc cggaacgata cctgctggct
     1141 gaccctggcc gaagctgtcg ggccggaggc cgcggcccgc cggacagcgt ggctgggcgc
     1201 gtaaggccgc tcgactagga tttccgccaa tgcggcggaa tcgtcttgtc gaaatacggc
     1261 agcgcctcga ttcgcgcctt ccatcgggcg agctgcggcg tcagcatgcc gtcagcgtcc
     1321 agatccggca gcttgatctg cgaccgccac acgaaagcga gcaaggtata aagcgcgtag
     1381 tcggccgccg acagcggtcc ggcaagatag tcgccgcgca tcgacttgct aacgagcgcc
     1441 agctcgtcga ccagcgcctt gcgcgagtcg gcgatggctt tggcgtcgcg ttcctcgggc
     1501 tttttgctga acgcctgcgt cgtgagcgga tccagcgccg catcgaaatc ctcatcgacc
     1561 tcgcagatca tgcgccggat gatcgcccgc gtcctggcgt cgcccgggaa cagcggcgca
     1621 ccgcggcccg ggtaggcttc gtcgaggtat tcgacgatgg cgctcgactc gtacaggacg
     1681 aaatcgtctt cgacgagcac cggcacgcga tgccgcggat tgagcgccag gaattcgggt
     1741 ttgcgtgtgt cgcccgccgc gaacgaaagt accttgagct cgtacggcaa cgccttgtgc
     1801 tcgagcgcga gctgggcgcg ccacgcataa ggcgaacccg atccgtaata gagcgtgata
     1861 gccatcgatt ctccttcgaa tcaagatcca gattttttct tgccaggctt tgccggcgcg
     1921 gtcgcggcgc gcatcgcctc cagcgaggct ttctgcagct ccatcgtctt gatgctcatc
     1981 tgcaggaagt tctgactcat gccgagccag ttttcgatca cgcgcaactc ggcgatcttc
     2041 ttgtcgatct cggcggggtc cagtgttgcg atcatcgccg ccggattcgg aaacggaatc
     2101 gtcgcggcgg ctgcgcctgg cgcgccgggt ggcgaaaagc ccggtaccgg tacaccgaac
     2161 ggattccaca tccgctgcat gaattccatc gcttcctgcg gcgtcaaagg catcgagggc
     2221 tgatccggca tggtcgatct cccgctgagc gcccgctacg atcgggcgtg atttccagta
     2281 ttatgcccca catgtttgac gctgccgcgt ccctgcttgt cacgctgaag tcgatcgtcg
     2341 gcgaggccaa tgttctcacc gccgacgcgg acgtcgcgtc gtatgtaacc gactggcgag
     2401 agcgctatcg cggccgcgcg cgcgcagtgg tccgcccggg atcgaccgac gaagtcgcgg
     2461 ccgtcgtacg ctgctgcgcc ggtcatcggg tgccagtcgt gccgcaggga ggcaacacgg
     2521 gactctgcgg cggcgccacg ccgcacgcga ccggcgacga ggtcgtgctg accttgacgc
     2581 ggatgaatcg cgtgcgcgcg atcgacgccg acgatgcgac gatgaccgtt gaagccggga
     2641 tgccgctggc cagcgttcag cag
//
LOCUS       DATANS010000670         1596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_707432, whole genome shotgun sequence.
ACCESSION   DATANS010000670 DATANS010000000
VERSION     DATANS010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707432"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VHT22_09900"
     CDS             <1..703
                     /locus_tag="VHT22_09900"
                     /inference="COORDINATES: protein motif:HMM:NF009828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEX3137912.1"
                     /translation="AARPSQGERIRKVLGAVPVVESPQALLKLKPELVVECASHEAFR
                     QYAEPMFSAGIDLIAVSVGVLADAAYRERVLAAARTSGATFQIPAGAIGGIDAISAAR
                     HAGLTRVVYVTRKSPRAWKGTPAETMIDLATVTAPKLFYDESAEHAATLFTEKANVTA
                     TLALAGIGFQATRVQFWVDPAVTQSVHHIEAEGPCGTLVIELANNVAADGKASLQTAM
                     SIVQAVRNRQAVLRV"
     gene            821..>1596
                     /locus_tag="VHT22_09905"
     CDS             821..>1596
                     /locus_tag="VHT22_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX3137913.1"
                     /translation="MATPWLEAARRDPAPFWAVETLLREYPISSAEGNALMRLAEALL
                     RVPDRETAIALTADQLGRAAFDAHSDSWIAALSARALALSKAMLPGSGEPAGPMGRLG
                     SEAVVAIALRAVQLLGRQFVLGERIDAAMVRADEWRAHYPAARFSYDMLGEGARTDGD
                     AQRYLEHYMEAVQALAGRGDSTQQAEERDGISIKLSALFPRYEDPQRGRVLRELVPRA
                     WSLCELAARAGINLTIDAEESERLELSLDVFEALAQRAAA"
BASE COUNT          273 a          498 c          552 g          273 t
ORIGIN      
        1 cgccgcgcgc ccgtcgcaag gcgagcgcat acgcaaggtg cttggcgcgg tgcccgttgt
       61 cgaatcaccg caggcgctgc tcaagcttaa gcctgaactc gtcgtcgaat gcgcgagcca
      121 cgaagcgttc cggcaatacg ccgaaccgat gttcagcgcg ggcatcgatc tgatcgcagt
      181 gtcggtcggc gtgctcgcgg atgccgcata tcgcgaacgc gtactggcgg cggcgcgcac
      241 gagtggcgcg acgttccaaa ttcccgccgg cgcaatcggc gggatcgacg cgatctctgc
      301 ggcacgccac gccggtttga cgcgcgttgt ctacgtgacg cgcaagagtc cccgtgcgtg
      361 gaaaggcacg ccggccgaaa cgatgatcga ccttgcaacg gtgactgcgc caaaattgtt
      421 ttacgatgaa tcggccgagc acgcggcgac gctgttcacc gagaaagcca acgtcaccgc
      481 gacactcgct ctcgccggca tcggcttcca ggcgacgcgc gtgcagttct gggtcgaccc
      541 ggcggtgact caaagcgtcc accatataga ggcggaaggt ccgtgcggaa cactggtcat
      601 cgagcttgcc aacaacgttg ccgctgacgg caaggcatcg ctgcagaccg cgatgagcat
      661 cgtgcaggcg gtgcgcaacc ggcaggccgt attgcgagtt tagctcatga caaatccggc
      721 aacgctgcgt ttgtcctcgc cctatcggcc ggaggcggaa gcggctgccg cttgtttgcg
      781 cggtattgac agcgcagtcg attggtccgc agttgcggca atggcaacgc catggctgga
      841 agccgcgcgc cgcgatccgg cgccgttctg ggcggtggag acgctgctgc gcgaatatcc
      901 gatttcgagc gccgagggta acgcgctcat gcggctggct gaagcgctgt tgcgcgtgcc
      961 cgatcgtgaa accgcgatcg cgctgactgc cgatcagctc gggcgtgccg cgttcgacgc
     1021 gcattcggac agttggatcg ctgcgctttc cgcccgcgcg ctcgcattgt ccaaggcgat
     1081 gttgccgggc agcggcgagc ccgccggacc gatgggccgg ctcggcagcg aggcggtggt
     1141 ggcgattgcg ctgcgcgccg tgcaactgct gggccgccag ttcgtgcttg gagaaaggat
     1201 agatgctgca atggtgcgcg cagatgagtg gcgcgcacac tatccggcgg cccgcttcag
     1261 ctacgacatg ctcggcgaag gcgctcgcac cgatggtgac gcgcagcgtt atctggaaca
     1321 ttacatggag gccgtgcagg cgctggccgg ccgcggtgat tcgacgcagc aggcggaaga
     1381 gcgcgacggc atttcgatca agctctccgc gttatttccg cgttatgaag acccgcagcg
     1441 cgggcgggtg ctgcgcgagt tggtgccgcg cgcgtggtcg ttgtgcgagc tagccgcgcg
     1501 cgcgggcatc aacctgacga tagacgcgga agaaagcgag cgcctcgaac tttcgctcga
     1561 cgtgttcgaa gcgctcgcgc agcgcgccgc cgccga
//
LOCUS       DATANS010000671         2318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_369932, whole genome shotgun sequence.
ACCESSION   DATANS010000671 DATANS010000000
VERSION     DATANS010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369932"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VHT22_09910"
     CDS             complement(<1..260)
                     /locus_tag="VHT22_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137914.1"
                     /translation="MIAILQIPARRAIGAALVTFATALLLAACASVPPPLSPPGNQVP
                     SPTGPGTEPTAPAKPQAPPTKNPNPPANVNLQGFPLPYRQGYA"
     gene            297..>2318
                     /gene="dnaE"
                     /locus_tag="VHT22_09915"
     CDS             297..>2318
                     /gene="dnaE"
                     /locus_tag="VHT22_09915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX3137915.1"
                     /translation="MSASAAVPLFIHLRLHSEYSIVDGMVRIDDAVDAAAADRMPALA
                     LTDLANVFGMVKFYKAARSRGVKPIIGCDVWITHEKERDAPHRLLLLCQSRAGYLRLA
                     DWLTRAHRANQHRGRPELRREWFADGTEGLIALSGFAGGEVGQALAQGNATAAGVAAT
                     ALASAFPGRFYLEVQRAGHSDDEALTAATLRLASTLALPVVATHPVQFATRGEFRAHE
                     ARVCIAEGHVLADPRRPRRFTPEQYFKSQAEMAQLFADVPQALANSVAIAQRCSLTIP
                     LGKNYLPAFPTPDGISIEQHLRDQAAAGLERRLAALYPDPEARAQRQPEYAARLEFET
                     RTIIQMGYAGYFLIVADFINWARSNGVPVGPGRGSGAGSLVAYSLGITDLDPLRYTLL
                     FERFLNPERVSMPDFDIDFCQDGRDRVIDYVKQKYGAESVSQIATFGTMAAKAVVRDV
                     GRVLDLPYSFCDGIAKLIPFQPGRAVTLKRRGSDAPPNVVYAREVEPLLNEREAAEEE
                     VRELLSLAEQLEGLPRNVGMHAGGVLIAPGKLTDFCPLYTQPGSDALVSQFDKDDVEL
                     VGLVKFDFLGLTTLTLDTLPLDDQRAYDIFRSGNTTAVFQFESRGMRELLTQAPPTRF
                     EDIIALVALYRPGPMELIPAYTARKTGRERFDYPDARLEPILGPTYGVMV"
BASE COUNT          375 a          768 c          788 g          387 t
ORIGIN      
        1 gcgtagcctt gccgatatgg caacggaaag ccctgcagat tgacgttggc ggggggattc
       61 ggattcttcg ttggcggcgc ctgcggcttg gcgggcgccg tcggttccgt gcccggtccc
      121 gtcggcgaag gaacctgatt gcccggcggc gacaacggcg gcgggaccga cgcgcaggcg
      181 gcgagaagaa gcgcagtcgc gaatgtgaca agtgcggcgc cgatggcgcg gcgcgccggt
      241 atctgcaaga tggcgatcat cggggtcggc ggctgaagcg agtaaaattg tacctcatga
      301 gcgcgtccgc ggccgtcccg ctgttcatcc atctgcgcct gcacagcgag tattcgatcg
      361 tcgacggcat ggtgcgcatc gacgacgccg tcgacgccgc cgcggccgat cgaatgccgg
      421 cgcttgcgct gaccgatctg gccaacgtct tcggcatggt caagttctac aaggcggcgc
      481 gttcacgcgg cgtcaagccg atcatcggtt gcgacgtctg gattacccac gaaaaagagc
      541 gcgacgcgcc gcatcgcctc ctgctgctct gccagtcgcg cgcgggctat ctgcggctgg
      601 ccgattggct gacccgcgcg catcgcgcca accagcaccg cggtcgcccg gaactgcggc
      661 gcgagtggtt cgccgatggc accgaaggcc tgatcgcgct gtcgggattc gccggcggcg
      721 aagtgggcca ggcgctggcg cagggcaatg cgacggccgc cggcgtcgca gcgactgcgt
      781 tggccagcgc gtttcccggc cgcttttatc tcgaagtgca acgcgccggc cattccgacg
      841 acgaagcgct gaccgccgcg acactgcggc tcgcgagcac gctggcgctg ccggtcgtcg
      901 cgacgcaccc ggtccagttc gcgacgcgcg gcgaatttcg cgcccacgag gcgcgcgtgt
      961 gcatcgccga ggggcacgtg ctcgccgacc cgcggcggcc gagacgtttc acgcccgagc
     1021 agtatttcaa atcgcaggcc gagatggcgc aactgttcgc cgacgtgccg caggcgctgg
     1081 cgaacagcgt cgcgatcgcg cagcgctgca gcctgacgat tccgctcggc aagaactatt
     1141 tgccggcgtt cccgacgccc gacggaatca gcatcgagca gcatctgcgc gaccaggcag
     1201 ccgcgggcct cgaacggagg ctcgccgcgc tgtatcccga tccggaggcg cgagcgcaga
     1261 ggcaacccga atatgcggcc cggctcgagt tcgagaccag gacgatcatt cagatgggtt
     1321 atgccgggta ctttctgatc gtcgccgact tcatcaactg ggcgcgaagc aatggtgtgc
     1381 cggtcgggcc gggccgcggt tccggcgccg gttcgctggt cgcctacagc ctcggcatca
     1441 ccgatctgga tccccttcgc tatacgctgc tgttcgagcg cttcctcaat ccggagcgcg
     1501 tgtcgatgcc ggacttcgac atcgatttct gccaggacgg ccgcgaccgc gtcatcgact
     1561 acgtcaaaca gaagtacggc gccgaatcag tttcccagat agcgaccttc ggcacgatgg
     1621 cggccaaggc ggtggtgcgc gacgtcgggc gcgtcctcga ccttccgtac agcttctgcg
     1681 acggcatcgc aaaactgatt ccattccagc ccggtcgcgc ggtgacgttg aagcggcgcg
     1741 gcagcgacgc gccgccgaac gtcgtctacg cgcgcgaggt cgagccgctg ctcaacgagc
     1801 gcgaggcggc cgaagaggaa gtgcgcgagc tcttgagcct ggccgagcag ctcgagggcc
     1861 tgccgcgcaa cgtcggcatg catgccggcg gcgtgctgat cgctccgggc aagctgaccg
     1921 atttttgccc gctttatacg cagccgggct ccgacgcgct ggtgtcccag ttcgacaagg
     1981 atgacgtcga gctcgtcggt ctggtcaagt tcgactttct gggactgacg acgctgacgc
     2041 tcgatacgct gccgctggac gatcagcgtg cttatgacat cttccgcagc ggcaatacga
     2101 cggcggtgtt ccagttcgaa tcgcgcggca tgcgcgagct gctgacgcag gcgccgccga
     2161 cacgtttcga ggacatcatc gcgctggtcg cgctgtaccg gccgggcccg atggagctca
     2221 tccccgctta cacggcgcgc aagacgggcc gcgagcgctt cgactatccc gacgcgaggc
     2281 tcgagccgat cctcggcccg acctacggcg tgatggtg
//
LOCUS       DATANS010000672         3802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_369951, whole genome shotgun sequence.
ACCESSION   DATANS010000672 DATANS010000000
VERSION     DATANS010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369951"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VHT22_09920"
     CDS             <1..987
                     /locus_tag="VHT22_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HEX3137916.1"
                     /translation="QIFWSSGIDVPDDAFARMIDEVTLEMVELPPGEKAMLSLTLPAE
                     FIIVFEPVTHCAQFIDVKGEPTRERQALTIVYSKTHAPHETLIMQPGPLRLTLENRSD
                     IRVVPGVYVAGQTMHDMLGQRRPFLTAKRLLTNQTFREIYRTSTLDVDQRLKITSLTF
                     LFTDLKGSTDLYERVGDLVAFDIVKEHFKILHEIIAAESGAVVKTIGDAVMATFATPD
                     RAIAAALRIRAAMGDLNQRRHNEDMLLKIGIHEGPCLAVTLNDRQDYFGQTVNIASRV
                     QALAVSHSILATGSVVDHPQSTELLQTDGIKPTSRRLPLRGIADEVAVYEIP"
     gene            997..1521
                     /locus_tag="VHT22_09925"
     CDS             997..1521
                     /locus_tag="VHT22_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137917.1"
                     /translation="MTASCYLCAIGNKTIPNTDAGDALGVCQICNVLACQGHAVRDAN
                     YPRWICVLCDLTLLTVAGVLQSGDEGAQRAALSDVSEEVIRAARSIRSARQYFRNRPR
                     DSWGWIQSAAEEYRGLPASALDSELSPMWRGMSDDGQEMIRCALAIVDKLDLRDSELI
                     GILRGVRAAVVRRV"
     gene            1514..2011
                     /locus_tag="VHT22_09930"
     CDS             1514..2011
                     /locus_tag="VHT22_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137918.1"
                     /translation="MSENFDARGHPGWTSSGQVSVPQQTWDKTSLRDARYVRITRSDN
                     QASSVFKVTPREAAGGNAVWVSPDQLGLAAKEVRAVTVEAASKNAYQWSQLVGTGWGQ
                     LTLIGLALGLIGIGIDAAFDIGKYKLLFPTSDAMAAWARLVAYFLKALGLLIVFLQAT
                     WFKKD"
     gene            complement(2037..2909)
                     /locus_tag="VHT22_09935"
     CDS             complement(2037..2909)
                     /locus_tag="VHT22_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137919.1"
                     /translation="MSHWLHKPATRQDVVTIRKVWLTLAVSLLVHMAALLLFVTHPPI
                     PGLEQQAVDQESNPIQVDLAEARPPSPSPPAARAEPAAEPLPKVARAAPRVRPPPMVA
                     MPRLEAPAIRTPALAPPPQIAAAPSPPQARPPAQGDFLSFVQANRRARGVVESAQDNS
                     EPDFNTKIAANLPGAAHGVAAQRSIRGGGIFQIKRMSYDDAAFEFFGWNNQMGRQTPQ
                     MIEVRKGNNPDMRIAVVRRMIVIIREHEREDFTWESGRHGGRIVTLSARLADTAALEK
                     FLLQDLFDDTREGP"
     gene            3154..>3802
                     /locus_tag="VHT22_09940"
     CDS             3154..>3802
                     /locus_tag="VHT22_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX3137920.1"
                     /translation="MSSALFTPYKLRKLALANRIVVSPMCQYSAVDGCATDWHLVHWG
                     QLLQSSAGMFTIEATAVSAIGRITYGCLGLYDDACEKALGERLVIARKHAPPMPVAIQ
                     LAHAGRKGSSQVPWEGGQLIPVAQGGWTPVAPSAIPQLEHETPPAALDAAGLMQIKSE
                     FVAAAKRAAQLGLDALELHCAHGYLLHEFLSPLSNRRDDAYGGSFDNRVRFPLEVF"
BASE COUNT          656 a         1207 c         1272 g          667 t
ORIGIN      
        1 cagatattct ggagctcggg catcgacgtg ccggacgatg cgttcgcccg gatgatcgac
       61 gaggtgacac tcgaaatggt cgagctgcct cccggcgaga aagcgatgct gtcactgacc
      121 ttgccggcgg aattcatcat cgtcttcgag ccggtcacgc attgcgcgca attcatcgac
      181 gtcaagggcg agcccacccg cgagcgccag gcgctgacga tcgtatacag caagacgcac
      241 gcgcctcacg agacgctgat catgcaaccc gggccgctgc gcctgacgct ggagaatcgc
      301 agcgacatcc gcgtcgtgcc gggtgtttat gtcgccgggc aaacaatgca cgacatgctt
      361 ggccagcgcc ggccgttcct gaccgccaag cgcctgctga ccaaccagac gttccgcgag
      421 atctaccgga ccagcacgct cgacgtcgat cagcggctca agataacgag tctcacgttc
      481 ctgttcaccg atctcaaggg ctcgaccgat ctgtacgagc gcgtcggcga tctggtggcg
      541 ttcgacatcg tcaaggagca cttcaagatc ctgcacgaga tcatcgccgc cgaatcgggc
      601 gcagtcgtca agaccatcgg ggacgcggtg atggcgactt tcgccacgcc cgatcgggcg
      661 atcgccgcgg cgctgcgtat ccgcgccgcg atgggcgacc ttaatcagcg ccggcataat
      721 gaggacatgc tgctgaagat cggcatccac gaggggccct gcctcgcggt cacgctgaac
      781 gatcggcagg actatttcgg ccagacggtc aacatcgcat cgcgcgtcca ggcgctcgcg
      841 gtgtcgcact cgatccttgc gacgggatcg gtcgtcgatc atccgcagtc gaccgagctg
      901 ctgcagaccg acggcatcaa gccgacgtcg cgccgcctgc cgttgcgcgg cattgccgac
      961 gaggtcgcgg tgtacgaaat accctaggga gccgcgatga ccgcaagctg ctacctttgc
     1021 gccatcggaa acaagacgat tccgaatacc gacgccgggg acgccctcgg cgtttgccag
     1081 atctgcaatg tgctcgcctg ccagggccac gccgttcgtg atgcgaacta tccgcgctgg
     1141 atctgtgtgc tttgcgatct gacgctgctc accgttgcag gagtcctgca gagcggcgac
     1201 gagggcgcgc agcgggccgc gttgagcgac gtctccgaag aggtgatccg ggcggcgcga
     1261 tcgatacgct cggcgaggca gtatttccgg aatcgcccga gggattcctg gggatggatc
     1321 cagagtgccg ccgaggaata ccggggcttg cccgcatcgg cgctcgacag cgagttgtcg
     1381 ccgatgtggc gcggcatgtc cgacgacggg caggagatga ttcgctgcgc gctcgccatc
     1441 gtcgacaagc tcgaccttcg cgattcggag ttgatcggga tactgcgagg ggttcgcgcc
     1501 gcggtagtac gacgtgtctg agaacttcga cgcgcggggc caccccggct ggacgtcgtc
     1561 gggacaggtt tcggtgccgc agcagacctg ggacaagacc tcgttgcggg atgcgcggta
     1621 cgttcggata acgcggagcg acaaccaggc ttcgagcgtg ttcaaggtaa cgccgcggga
     1681 agcggctggc gggaacgcgg tttgggtgag tcccgaccag ctcggtctgg cggcgaaaga
     1741 agtccgtgcc gtcacggtcg aggccgcgag caagaacgcg tatcagtgga gtcagctggt
     1801 cggcaccgga tggggccagc tgacgctgat cggtctcgcg ctgggcctga tcgggatcgg
     1861 catcgacgcc gcattcgaca tcggcaagta caagctgctg tttccgacca gcgatgccat
     1921 ggcggcctgg gcccggctcg tcgcctactt cctcaaggct ctcggcctgt tgatcgtctt
     1981 tctgcaggct acctggttca agaaggactg acgacgggct gccgaccaat tccgcgtcag
     2041 ggtccctcgc gggtgtcgtc gaacaggtct tggagcagaa atttttcgag cgccgccgtg
     2101 tcggccagtc gcgccgacaa ggtaacgatg cggccgccgt gacgccccga ctcccaggtg
     2161 aaatcttcgc gctcgtgctc gcggatgata acgatcatcc tgcgcaccac ggcgatgcgc
     2221 atgtcaggat tgttgccctt gcgcacttcg atcatctgcg gcgtctgccg gcccatctgg
     2281 ttattccatc cgaaaaactc gaacgccgca tcgtcgtagg acatccgctt gatctggaag
     2341 atgccgccgc cgcggatcga ccgctgcgcg gcgacgccgt gcgcggcgcc aggaagattg
     2401 gcggcgattt tggtattgaa gtcgggctcg gaattgtcct gcgctgactc gactacgccg
     2461 cgcgcgcgtc gattggcctg cacgaacgaa agaaaatcgc cttgcgccgg gggtctcgct
     2521 tgcggcggcg acggagccgc ggcgatctgc gggggcggag cgagcgccgg cgttcgaatg
     2581 gccggcgcct ccaggcgcgg catcgcaacc atcggtggcg gtcgtacgcg gggtgccgcg
     2641 cgcgccacct tcggtagcgg ctccgccgca ggctcggcac gagcggccgg cggcgacggc
     2701 gatggcggtc tcgcctcggc aaggtcgacc tggatcgggt tgctttcctg atcgaccgcc
     2761 tgctgctcga ggcctgggat cggcggatgg gtgacgaata gcagcagcgc cgccatatgg
     2821 acgagcaacg ataccgccag cgttaaccac accttccgaa tggtcacgac gtcctgccgg
     2881 gtggcaggct tgtgcaacca atggctcatc ggccactcgt cgggcggtgg cggtggccgc
     2941 gggttgggga tggaggtaag ggtggtcatc ggcttgggat acgagcgccg gctctctaat
     3001 agtcgcaccg gagaatccac agattcaata cgagccagag gcgggcgatt cgttccctcc
     3061 cccggcagcg cgctgtcagc tcccagtccg gatcgcgctg agcccaaaat cggctacgct
     3121 cgcggttttg ccgccagccc ccccatctta gcaatgtcct cagccctgtt cacgccgtac
     3181 aagctgcgca agctcgcgct cgccaaccgc atcgtcgttt cgccgatgtg ccagtactcg
     3241 gccgtcgacg gctgcgcgac cgattggcac ctcgtccact ggggccagtt gctgcaatcg
     3301 agcgcgggaa tgttcacgat cgaggcgacc gcggtgtcgg cgatcggacg aatcacctac
     3361 ggctgcctgg ggttgtacga cgacgcctgc gaaaaagcgc tgggcgagcg cctggtgatt
     3421 gcgcgcaagc acgcgccgcc gatgccggtg gcgattcagc tcgcgcacgc ggggcgcaag
     3481 ggctcgtcgc aagtgccgtg ggaaggcggg cagctgattc cggtcgcgca aggcgggtgg
     3541 acgccggtgg cgccgtcggc gataccgcag ctcgagcacg aaacgccgcc ggccgcgctc
     3601 gacgcggcag gcctgatgca gatcaagagc gaattcgtcg ccgccgcgaa gcgggcggcg
     3661 caactcggtc tcgatgcgct cgagctgcat tgcgcccacg gttacctgct gcacgaattt
     3721 ctttcgccgc tatcgaaccg gcgcgacgac gcttacggcg gttcgttcga caatcgggtg
     3781 cgctttcccc tcgaagtctt cg
//
LOCUS       DATANS010000673         3833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_328830, whole genome shotgun sequence.
ACCESSION   DATANS010000673 DATANS010000000
VERSION     DATANS010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328830"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VHT22_09945"
     CDS             <1..357
                     /locus_tag="VHT22_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEX3137921.1"
                     /translation="DAMAVCPCSMGTLAAIAHGLADNLIERAADVMLKERRTLVLVPR
                     ETPLSAIHLENMLKLSRAGAVILPPSPGFYAHPRTIEDLVDFVVARVLDQLGVEHALG
                     PRWGAARGATPRFERA"
     gene            366..998
                     /locus_tag="VHT22_09950"
     CDS             366..998
                     /locus_tag="VHT22_09950"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX3137922.1"
                     /translation="MMPFRRGAPSREPLTLPLFPLKTVLFPGGLLPLKVFEQRYIDMT
                     KRCIQDDHPFGVCLLTHGEEVARRGAPETPEFALIGTLARITSWDMPHLGILQLKTEG
                     GSRFQVHSHRTGDDGLVLAQATSILPEPKVPLPEMHQPLAGLLELMINRVGREHFVGE
                     PALDDASWVGYRLAELLPLPANIKQSMLEINDSEVRLKAIAQFLKQQDLL"
     gene            1080..1805
                     /locus_tag="VHT22_09955"
     CDS             1080..1805
                     /locus_tag="VHT22_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX3137923.1"
                     /translation="MAGHSKWANIKHKKAATDAKRGKIFTRLIREITVSSKLGGVDAS
                     TNPRLRLAVDKAMDNNMPKDTIERAIKRGSGGADDASYEEIRYEGYGINGAAVIVDCL
                     TDNRTRTVADVRHAFSKNGGNLGTDGSVAFLFRHCGQIVFAPGTDENRLIELALDAGA
                     EDVVSHDDGSSEVITAPHDLVRVRQLLAEAGLKSDFAEVAMKPTVEVECRGEDAVRMQ
                     KLLDALEALDDVQDVYTTAVIVE"
     gene            1802..2341
                     /gene="ruvC"
                     /locus_tag="VHT22_09960"
     CDS             1802..2341
                     /gene="ruvC"
                     /locus_tag="VHT22_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX3137924.1"
                     /translation="MNQITLARRILGIDPGLRVTGFGVIICTGNRLQYVASGCIRNTS
                     GELPDRLKLIVRDLATVIGAHLPTEVAVEKVFVNVNPQSTLLLGQARGAAISAAVLAE
                     LPVSEYTALQIKQAVVGHGKAAKEQVQEMVRRLLALPSAPSADAADALACAICHAHGG
                     AGIGGMRRGGYRVRRGRIL"
     gene            2338..3006
                     /locus_tag="VHT22_09965"
     CDS             2338..3006
                     /locus_tag="VHT22_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX3137925.1"
                     /translation="MIERWPEAVIFDMDGLMLDTEGPALRAWESAARTLGREFDLELC
                     RQMIGRNFGDCVALIRARHGSDYPVDQLTSAWAADYDAIVAAEGISLKPGLDDILDLL
                     DERGIAIAVATSTRRERARAKLDRVGLLHRFAALVGGDEVARGKPAPDIFIAAAERLG
                     VAARECIVLEDSEPGIIAAAAAGMTPVMVPDLHPPSAELLLLAPLVLSSLSDVAAHLR
                     AIGR"
     gene            3136..3717
                     /gene="ruvA"
                     /locus_tag="VHT22_09970"
     CDS             3136..3717
                     /gene="ruvA"
                     /locus_tag="VHT22_09970"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX3137926.1"
                     /translation="MIGRLAGTLLEKNPPQILLDVQGVAYEVDVPMSTFYNLPGIGDR
                     LTLFTHLVVREDAHLLFGFGSENERRAFRQLIRISGVGARIALAVLSGLSVAELAQAV
                     SMQDSGRLTKIPGIGKKTAERLLLELKDKLGADLTTAVGVYRPAPVASDVLNALLSLG
                     YSDKEASRAVRELPEGIAVGDGIRQALKLLAKA"
BASE COUNT          651 a         1272 c         1275 g          635 t
ORIGIN      
        1 gacgcgatgg cggtgtgccc gtgcagcatg ggcacgctgg ccgcgatcgc acacggtctg
       61 gccgacaacc tgatcgaacg cgccgccgac gtgatgctca aggagcggcg gaccttggtg
      121 ctcgtgcccc gcgagacgcc gctttcggcg atacatctgg agaatatgct caagctatcg
      181 cgagccggcg cggtgatcct gcctccatcg cctggctttt acgcgcatcc gcgcacgatc
      241 gaggatctcg tcgacttcgt cgtcgcacgt gtgctcgacc agctaggcgt cgagcatgcg
      301 ctcggacctc gctggggcgc agcgcgcggc gcaacgccac gcttcgagcg cgcttaaggc
      361 cggcaatgat gcccttccgg cgcggcgcgc catcgcgcga gccgctgacg ctgccgctgt
      421 ttccgctgaa gacggtactg tttcccggcg gcctgctgcc cctgaaggtc ttcgagcagc
      481 gctacatcga catgaccaag cgctgcatcc aggacgacca tcctttcggc gtgtgcctgt
      541 tgacgcatgg cgaggaagtc gcgcgtcgcg gcgctcccga gacgccggag ttcgcgctga
      601 tcggcacgct ggcgagaatc acgtcctggg acatgcccca cctcggcatc ctgcagctca
      661 agaccgaggg cggcagccga ttccaggtgc actcgcatcg caccggcgac gacggcctcg
      721 tgctggcgca ggcgacctcg atcctgccgg agccgaaagt cccgttgccg gagatgcacc
      781 aaccgctcgc gggattgctc gagctgatga tcaatcgcgt cgggcgcgag cacttcgtgg
      841 gcgagccggc actcgacgac gcttcttggg taggttaccg actcgccgag ctattgccgt
      901 tgccggcgaa catcaagcag agcatgctcg agatcaacga cagcgaagta cggctcaagg
      961 cgatcgcgca atttctcaag cagcaggatc ttctataggg gccgcctgcg tcacgcgccg
     1021 tcgttcgcag catgcccgct gctataattt gcgctctggc agcatcgcgg gaacgcatca
     1081 tggcaggcca cagcaagtgg gcgaacataa agcataagaa ggctgccacc gacgccaagc
     1141 gcggcaagat cttcacccgg ctgatccgcg aaatcaccgt ctcttcgaag ctcggcggcg
     1201 tcgatgcctc gaccaatccg cggctgcggc ttgcggtcga caaggcgatg gacaacaaca
     1261 tgccgaagga cacgatcgag cgcgcgatca agcgcggctc gggcggcgcc gatgacgcga
     1321 gctacgagga gatccgttac gaaggctacg gcatcaatgg cgcggcggtg atcgtcgact
     1381 gcctgaccga caaccggacg cgcaccgtcg ccgacgtgcg ccatgcgttt tccaaaaacg
     1441 gcggcaatct cggcaccgac ggctcggtcg cgttcctgtt caggcactgc ggccagatcg
     1501 tgttcgcgcc gggcaccgac gaaaacaggc tgatcgaact tgcgctggac gcaggcgccg
     1561 aggacgtcgt cagccatgac gacggctcgt cggaagtgat caccgcgccg cacgacctgg
     1621 taagagtccg gcagctgctg gccgaggccg gcctcaaatc cgatttcgcc gaagtcgcga
     1681 tgaagcccac ggtggaagtc gaatgcaggg gcgaggatgc cgtccggatg cagaagctgc
     1741 tggacgccct cgaagctctc gacgacgtgc aggacgtcta cacgacggca gtcatcgtcg
     1801 aatgaaccag atcacattgg cccgccggat attgggtatt gaccctggtc tccgggtgac
     1861 aggttttgga gtgatcatct gcaccggcaa ccgcctccag tacgtggcca gcggctgcat
     1921 acgcaatacc tcgggcgagc tgcccgatcg gctcaagctg atcgtccgcg accttgccac
     1981 cgtcatcggc gcgcaccttc ccaccgaggt cgcagtcgaa aaagtctttg tcaatgtcaa
     2041 tccgcagtcg accttgctgc tcggtcaggc gcgcggcgcg gcgatctcgg cggccgtgct
     2101 tgccgagctt ccggtcagcg aatacacggc gctgcagatc aagcaggcgg tcgtgggtca
     2161 cggcaaggcc gcgaaggagc aggtgcagga gatggtgagg cgcctgctcg cactgccgtc
     2221 ggcgccttcc gccgatgcgg ccgacgcgct cgcctgcgcc atctgtcacg ctcacggcgg
     2281 cgccggcatc ggcggcatgc gccgcggcgg ctaccgcgtg cgcagaggcc gcatcctttg
     2341 atcgagcgct ggccggaagc cgtcattttc gacatggacg gcctgatgct cgacaccgag
     2401 ggccctgctc tccgcgcctg ggagagcgcc gcgcgcaccc tgggtcgcga gttcgacctc
     2461 gagctctgcc ggcagatgat cggacgaaac ttcggcgatt gcgtcgcgct gatccgtgcc
     2521 cggcacgggt ccgattatcc ggtcgaccaa ttgacgagcg cgtgggcggc cgattacgac
     2581 gcgatcgtcg ccgccgaagg gatctcgctc aagcccgggc ttgacgacat cctggatctg
     2641 ctcgacgagc gcggcatcgc catcgcggtc gcgacctcga cgcggcgcga acgcgcgcgc
     2701 gccaagctcg atcgggtcgg actgctgcat cgcttcgctg cgctggtggg cggcgatgaa
     2761 gtcgcgcgag gaaagcctgc gcccgacatt ttcattgccg ccgccgagcg gcttggcgtc
     2821 gcggcccgcg agtgcatcgt cctggaggat tcggagcccg gcatcatcgc cgccgcggcc
     2881 gccggcatga cgccggtgat ggtgcccgat ctgcatccgc ccagcgccga gctgcttctg
     2941 cttgcgccgc tggtgttgtc ctccctttcc gacgtcgccg cgcatctgcg cgcgatcggg
     3001 cgatgagtcg cggccgccgg cgcaggcctc ggtgcatgcg ataattcgcg tcatgcgcgg
     3061 cattcgtctg cccgattttc ccgacaccca acgccggttg cgccggagcg cgcgcatcgc
     3121 ggcggcgacg acgcgatgat cggccgcctc gccgggacgc tgctggagaa gaatccgccg
     3181 cagatcctgc tcgacgtcca gggcgtcgcc tacgaagtcg acgtaccgat gagcacgttt
     3241 tacaacctgc ccggcatcgg cgacaggctt acgcttttca cgcacctggt cgtgcgcgag
     3301 gacgcgcatc tgctgttcgg tttcggttct gagaacgagc gccgtgcgtt ccgccagctg
     3361 atccggatca gcggcgtcgg agcgcgcatc gcccttgccg tgctgtccgg attgtcggtc
     3421 gccgagctcg cgcaggcggt cagcatgcag gacagcgggc ggcttaccaa gatccccggc
     3481 atagggaaga agacggccga gcggctgctg ctcgagctca aggacaagct aggcgccgat
     3541 ctgacgaccg cggtcggcgt atatcggccg gctcccgtgg cctccgacgt gctgaacgcg
     3601 ctgctgtcgc tcggctacag cgacaaggag gcctcacgcg cggtcaggga actgcccgaa
     3661 ggcattgccg tcggcgacgg gatcaggcag gccctgaagc tgctggcgaa ggcatgacga
     3721 tgcgctcgcc gatgcgcgcg aagcgcggcg gcgctgcagg cataatagac gcatgatcga
     3781 gaccgatcgc ctgatcgccg cggcgccggt gagcccgcag gaggaagtgc tcg
//
LOCUS       DATANS010000674         2998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_496406, whole genome shotgun sequence.
ACCESSION   DATANS010000674 DATANS010000000
VERSION     DATANS010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496406"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VHT22_09975"
     CDS             complement(<1..185)
                     /locus_tag="VHT22_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137927.1"
                     /translation="MTRTLAGTAAFRGYALRQATAGDEGFLYELHREAMREYVEITWG
                     WNEAWQRTHFATSYSPL"
     gene            complement(190..411)
                     /locus_tag="VHT22_09980"
     CDS             complement(190..411)
                     /locus_tag="VHT22_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137928.1"
                     /translation="MAEKKAFLLRLDPALWTALDRLAQSELRSVNGQIEFLLRDSLSR
                     RGIGPGREPTATPSAEAASDHRSRRGKVD"
     gene            complement(444..941)
                     /locus_tag="VHT22_09985"
     CDS             complement(444..941)
                     /locus_tag="VHT22_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137929.1"
                     /translation="MRRPALFVLVALIAVSAAFIAGTAGQLPSLVASHFDSGSGVNGW
                     LTRNQYLVWMLALAILLPLVIVGLIALIPRAAPRLINLPHRAYWLSEARREQTLATLL
                     SFGCAQGAVLTVFAAALHYVILQANTTAPPQLPGVLFVAVLAGLLAAMAAWTIALYVR
                     FRHVD"
     gene            complement(972..1148)
                     /locus_tag="VHT22_09990"
     CDS             complement(972..1148)
                     /locus_tag="VHT22_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004975817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137930.1"
                     /translation="MIHVRASNPGLWSAVGAGLGTAVGTALGNSPFGLAVGAALGMLF
                     AMLNFRGRARKARR"
     gene            complement(1175..2107)
                     /locus_tag="VHT22_09995"
     CDS             complement(1175..2107)
                     /locus_tag="VHT22_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX3137931.1"
                     /translation="MQQIGERKNAHQERAIVTWNGFAALAIGLLLLAAGIWQLLRGMP
                     YSGPPSMSGLLAAILTFVALFMLGLLILAGLDTLQPNEAVILQLFGSYRGSSRVPGLR
                     GTNPFYSRRKISLRARNLNGERLKVNDKRGNPIEIAAVVVWRVDDTARASFDVDNYEN
                     YVKLQSEAAVRHLASSFAYDHADALAGNMPEPTLLASADTVSRALVVELQQRLDKAGV
                     VVEEARLMHLAYAPEIAQVMLRRQQAEAIIAARTKIVHGAVSMVEMALRELSEKQVVA
                     LDDERKAAMVSNLMTVLCAESEVQPVINTGTLYN"
     gene            complement(2300..>2998)
                     /locus_tag="VHT22_10000"
     CDS             complement(2300..>2998)
                     /locus_tag="VHT22_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX3137932.1"
                     /translation="SRSGGSQGLAFAIPIDVAMQVEQQIVKHGKVSHGRLGVAIQDVN
                     QALAANFGLKSPKGALVSSVQKDGAGAKAGLKPGDVILTLNGEEIAGSSDLPPKIASL
                     SPGASVTLGIWRDGAEKHLTATLGSIDDGKNIVASGGKQDLGNARLGIAVRPLTAEER
                     RDNDIAGGLVVQDVAGAAERAGVRPGDVIVAVNTQPVRTVEELKSMVAKSGKTVALLV
                     QRDNAQIFIPVTLG"
BASE COUNT          514 a         1016 c          964 g          504 t
ORIGIN      
        1 tgcagcggcg agtagctggt cgcgaaatgc gtgcgctgcc aggcctcgtt ccagccccag
       61 gtgatctcga cgtactcgcg catcgcttcg cgatgcagct cgtagaggaa cccctcgtcg
      121 ccggcagtcg cctgtcgcag cgcatagccg cggaacgcgg cggtccccgc cagcgtcctc
      181 gtcaaagggt caatccacct tgccacgcct gctccgatgg tccgatgcgg cctcggccga
      241 cggcgttgcg gtcggctcgc ggcccggtcc gatgccgcgg cgcgatagcg agtcgcgcag
      301 cagaaattcg atttgtccgt tgacgctgcg aagctcgctt tgcgccaggc ggtcgagcgc
      361 cgtccagagc gccgggtcga ggcgcagcag aaaagccttt ttttcagcca cgtcggcagt
      421 gctcgcgtcc gcggcgcgcc gagtcaatcg acatggcgaa accggacata gagcgcgatc
      481 gtccacgccg ccattgcagc cagcagtccc gccagaacgg ccacgaacag cacgcccgga
      541 agctgcggcg gggcagtcgt attcgcctgc agtatgacgt aatgcagcgc ggcggcgaac
      601 accgtcagca cggcgccttg cgcgcagccg aaggacagca gtgtcgccag ggtttgctcg
      661 cgacgagcct ccgacagcca gtacgcgcga tgcggcaggt tgatcagccg cggcgcggcg
      721 cgcgggatca gcgcgatgag gccgacgatg acgagcggca acagtatcgc cagtgcgagc
      781 atccagacca gatattgatt ccgggtcagc cagccgttga ccccggagcc actgtcgaag
      841 tgcgaagcga ccaggctggg caattgaccg gcggtgcccg cgatgaaggc cgcgctgacg
      901 gcgatcagcg cgacgagcac aaaaagcgcg ggtcggcgca tgttgttctc ctgttgaagt
      961 gaactccgat gctatcgccg cgcttttcgc gcccggccgc gaaagttgag catggcgaac
     1021 agcatgccca gcgccgcccc gaccgcgagg ccgaacgggc tgttgcccag cgccgtcccg
     1081 acggcggtgc ccagtcccgc gccgacggcg gaccacaaac ccggattgct ggctcgcacg
     1141 tggatcatcg ttcgctcctt gggttgattc gggatcagtt gtacaaagtc ccggtattga
     1201 tgaccggctg gacttcgctt tccgcgcaaa ggacggtcat caggttgctg accatcgcgg
     1261 ccttgcgttc gtcgtcgagc gcgacaacct gcttttcgga cagctcgcgc agcgccattt
     1321 ccaccatcga caccgcgccg tgcacgattt tcgtccgcgc ggcgatgatc gcctcggcct
     1381 gctgccggcg cagcatcacc tgcgcgatct caggcgcgta cgccagatgc atcagccggg
     1441 cttcctcgac aacgacgccg gccttgtcca ggcgctgttg caactcgacc accagtgcgc
     1501 gcgagacggt gtcggcgctc gccagcagcg ttggttccgg catgttgccg gccagcgcgt
     1561 cggcatggtc gtaggcgaac gacgaggcca gatgccgtac cgcggcctcg ctctgcagct
     1621 tgacgtaatt ttcgtagttg tcgacgtcga aactggctcg cgccgtgtcg tcgacgcgcc
     1681 agacgacgac cgcggcgatt tcgatcggat tgccgcgctt gtcgttgacc ttgagccgct
     1741 cgccgttgag attgcgcgcg cgcagcgaga tcttgcgccg gctgtagaag ggattggtgc
     1801 cgcgcagccc cggaacccgt gagcttcctc gatagctgcc gaacagctgc aggatcaccg
     1861 cctcattcgg ctgcagcgta tccaagcccg cgaggatcaa gaggcccagc atgaacaacg
     1921 cgacaaaggt gagtatcgcg gcaagcaggc ccgacatcga cggcgggccg ctgtagggca
     1981 tgccgcgcaa cagttgccag atgccggccg ccagcagcag cagcccgatc gccagcgcgg
     2041 cgaatccgtt ccaggtgacg atcgcgcgct cctggtgcgc gttcttgcgc tcgccgatct
     2101 gttgcatgcg tgccctccga cagtggacgc ggccgccgca ttcgcgcgac cgatattgtt
     2161 atgatatcac aacaatatcg tccgatgtca agaccctgcc gcgaaccgca gtgaaggcga
     2221 aaaaaagagc ggacggggga ccgtccgctc ggggaccgaa gccggggagt caccggcacc
     2281 gggggagaac taagccgatt tagccaagcg tcaccgggat gaagatctgc gcgttgtcgc
     2341 gctgtaccag cagggcaacg gtcttgcccg acttggcgac catcgacttg agctcttcga
     2401 cggtcctgac cggctgggta ttgaccgcaa cgatcacatc gcccggccgg acgcccgcgc
     2461 gttcggccgc gcccgcgacg tcctgcacga ccagcccgcc ggcgatgtcg ttgtcgcgcc
     2521 gctcttcggc ggtcaacggc cgcacggcga tgccgagccg cgcgttgccc agatcctgct
     2581 tgccgcccga ggcgacgatg ttcttgccgt catcgatcga gcccagtgtc gcggtcagat
     2641 gcttttccgc gccgtcgcgc cagatgccca gcgtcacgct cgcaccggga gaaagcgatg
     2701 cgatcttcgg cggcagatcc gacgagcccg cgatctcttc accgttcaac gtgaggatca
     2761 cgtctcccgg cttgaggccc gccttggcgc ccgcgccatc cttctgcacc gagctgacca
     2821 gcgcgccctt cggcgacttc aggccgaagt tcgcggccag cgcctgattg acgtcctgaa
     2881 tcgcgacgcc cagcctgccg tgcgaaacct tgccgtgctt gacgatctgc tgctcgactt
     2941 gcatcgcgac gtcgatcggt atcgcgaacg cgagaccctg cgacccgccg gaacggct
//
LOCUS       DATANS010000675         4783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_117518, whole genome shotgun sequence.
ACCESSION   DATANS010000675 DATANS010000000
VERSION     DATANS010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117518"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VHT22_10005"
     CDS             <1..463
                     /locus_tag="VHT22_10005"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX3137933.1"
                     /translation="ENPPANAAPAPAVKVEMDQNGCVFIPRVVVVPAGGTVDFLNSDR
                     LLHNIHATPKLNVSFNRTQPKDRTIPVTFAKPEIVRINCDLHSWMIGWVVVAAHRYYA
                     ITGADGQFSFDNLPPGKYDLHIWHERLGTAQATATVGDQQPARVTVEMKAR"
     gene            complement(473..919)
                     /locus_tag="VHT22_10010"
     CDS             complement(473..919)
                     /locus_tag="VHT22_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HEX3137934.1"
                     /translation="MNNVTNVRLRAVSAALLLALFGLATLVSAAEEQAEQFTPEFRQL
                     HTSFDAKHSPKIVAPDAVKRGEWFTVTVSVGAGSQHPSLQEHFVRYIALYKDSVEIAR
                     TYLHPVYSAPIVTFTVSLDEGGSLRAVEEPTHSAAWETSKKIEVRP"
     gene            complement(942..1316)
                     /locus_tag="VHT22_10015"
     CDS             complement(942..1316)
                     /locus_tag="VHT22_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3137935.1"
                     /translation="MSERTILYIEDNEYNRKIVRQLLGRTSYQLVEAVDGESGVALAQ
                     QIVPQLILMDVQLPKMSGLDATRALKADPRTSDIPIIVITSFALSGDREKAAVAGADN
                     YLAKPYSPRELLALVRQYLPEA"
     gene            complement(1313..2995)
                     /locus_tag="VHT22_10020"
     CDS             complement(1313..2995)
                     /locus_tag="VHT22_10020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3137936.1"
                     /translation="MHKDEAGTKEMGHGIQSSLDNRSSTTRPELALEHLADLVQRRTP
                     LHRLVVWVAGIRASVHTKLLAAFLIATLLFIAVSLFSLQTLVNATQQSRQLDEAHELV
                     SLAQQSERALARQMHYTDVALLSQDEAAIARILRENNQFNDRLAKLESAGTADRTLVE
                     QIRTSQDEAMAVVADIANAIRDGKLGAVTGPLLHRQERLDTEITTRVGLLVAAQQDRM
                     TRLRDSVAAANKRSLILTSAFAVIAVIFALFCGFVISWSFILPVREAQGFLDEVAAGD
                     FGARIEVSNRDEFGVLAERMNHMSAELQRFDGEQRHAAAQLGHLNRQLAQASKAKSEF
                     LANMSHELRTPMNAILGFTEMLIDGLYGDIPPELKEPLTDIQLNGRHLLRLINDVLDL
                     SKIEAGRMQLALGDYSVREIIDIVQVSLRSLAAEKGLEFVTSVPDGLPVAYGDNGRLT
                     QCLMNLAGNALKFTRQGRVEIGVELVGTELVFRVSDTGIGIPKEDLDKVFTEFGQVDT
                     AVTREFGGTGLGLSITKRFVEMHGGRVWVESEFGKGCSFYFAVPVRAKGEET"
     gene            3043..4158
                     /locus_tag="VHT22_10025"
     CDS             3043..4158
                     /locus_tag="VHT22_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3137937.1"
                     /translation="MRNTPRILIVDDNPTNVKVLQTRLVAEGYEVVTAADGEEGLAVA
                     RRDKPDLILLDVMMPKLDGFEVCRRLRADASFPFTPIIMVTAMADSKDIVAGLEAGGD
                     EYLTKPVDHASLAARVRSMLRIKRLHDTAEELADEVKQWNTKLEERVAAQVSELERVG
                     RLRRFFSPHLAELIVSGGADDPLKSHRREITVVFLDLRGFTAFAEVAEPEEVMGVLRE
                     YHQIAGRMVLEYEGTLEHFTGDGITILFNDPVPIPNPAERAVRMALAVRERVGRMAQD
                     WTKRGYDLSLGIGIAQGFATIGAIGFEGRWDYSAIGTVTNLASRLCGEAPGDQILISQ
                     KVYAGVEALIEVEPQEALTLKGFRRPVIAYRVRGLKA"
     gene            complement(4219..>4783)
                     /locus_tag="VHT22_10030"
     CDS             complement(4219..>4783)
                     /locus_tag="VHT22_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003600583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX3137938.1"
                     /translation="SATKYLNGHSDVVAGALATARIDEFWERVKRIRTMLGMILGPLE
                     AYLLIRGMRTLHLRVAQVCVSAMELARRLQKHPAVAEVLYPGLPMHPGHAIAARQMRD
                     GFGGMLSIRVRDGEPAAIAAAANVRLWKRATSLGGIESLIEHRASIEGAGSPCPTDLL
                     RLSTGIEDVDDLFGDLDRALTAATRTS"
BASE COUNT          863 a         1564 c         1501 g          855 t
ORIGIN      
        1 cgagaatccg ccagcgaacg ccgcgccggc gcctgccgtc aaagtcgaga tggaccagaa
       61 cggatgcgtc ttcattccgc gggtggtggt cgttcccgcc ggcggcaccg tcgactttct
      121 caacagcgac cggctactgc acaacattca cgcgacgccg aagctcaacg tctcgttcaa
      181 tcgcactcag cccaaggacc gcacgattcc agtgactttc gccaagccgg agatcgtgcg
      241 catcaactgc gacctgcatt cgtggatgat cggctgggtg gtcgtcgcgg cgcaccgtta
      301 ctacgcgatc acaggcgcag acggacagtt cagcttcgat aatctgccgc cgggcaaata
      361 cgatctccac atctggcacg agcggctggg aaccgcccag gccaccgcga ccgtcggcga
      421 ccagcagccg gcgcgcgtca ccgtggaaat gaaggcgcgt tgaccggttt acctacggcc
      481 ggacctcgat ttttttcgat gtctcccagg ccgccgaatg cgtaggctct tcgaccgcgc
      541 gcagtgagcc gccctcgtcc agcgagacgg tgaaggtcac gattggcgcc gagtagaccg
      601 gatgcagata ggtgcgcgcg atctccaccg aatccttgta cagcgcgata tagcggacga
      661 agtgctcctg gagcgaggga tgctggctgc cggcgccgac gctgaccgtg acggtgaacc
      721 actcgccccg cttgaccgca tcgggagcaa cgatcttcgg cgagtgcttg gcgtcgaagc
      781 tcgtgtggag ctgccggaac tccggcgtga actgctcggc ctgctcctcc gcggcgctga
      841 caagcgtagc gaggccaaag agagccagca gcagcgcagc cgaaacggcg cgcagtcgaa
      901 cattcgtgac gttgttcatc cgatcacctc caatgcattg atcaagcttc cggtagatat
      961 tgccggacca gcgccagaag ctcgcgcggg ctgtaaggct tcgcgaggta gttgtcggcg
     1021 ccggccacgg ccgccttttc ccggtcaccg ctgagcgcga acgacgtgat cacgatgatc
     1081 ggtatgtcgc tggttcgtgg gtctgccttc agcgcacggg ttgcgtccaa cccggacatt
     1141 ttgggcagct gtacgtccat caggatcagc tgcggcacga tctgctgcgc cagcgcaacg
     1201 ccgctttctc cgtcgactgc ttcgacgagc tggtaggaag tacggcccag caactgacgg
     1261 acgatcttgc ggttgtattc gttgtcctcg atgtagagaa tcgtgcgttc gctcatgttt
     1321 cctcaccctt ggcccgcacc gggaccgcga aataaaagct gcatcctttg ccgaattcgc
     1381 tctcgaccca aacgcggccg ccgtgcatct cgacgaatcg cttggtgatg ctcaggccca
     1441 gacccgtacc cccgaattca cgggtcaccg cggtatcgac ctgcccgaac tcggtgaaca
     1501 ccttgtcgag gtcctccttc ggaatgccga tcccggtatc ggaaacgcgg aagacgagct
     1561 cggttccgac caactcgacg ccgatctcga cgcgtccctg ccgggtgaac ttgagggcgt
     1621 tgccggcgag gttcatcagg cactgcgtca gccgcccatt atcgccgtag gcgaccggga
     1681 gaccgtccgg cacgctggtt acgaactcca gccccttctc cgcggccagc gaccgcaacg
     1741 agacctgcac gatgtcgatg atctcgcgca ccgagtagtc gcccaaagcg agctgcatac
     1801 ggccggcctc gatcttggac agatcgagca cgtcattgat caggcgtagc agatggcgtc
     1861 cgttcaactg gatgtcggtc agcggctcct tcaactcggg cgggatgtcg ccataaaggc
     1921 cgtcgatcag catctcggtg aagccgagga tggcgttcat cggcgtccgc agctcgtggc
     1981 tcatgttggc gagaaactcg gacttcgcct tgctcgcctg cgccagctgc cgattgaggt
     2041 ggccgagctg cgcggcggcg tgccgttgct cgccatcgaa ccgctgcaac tccgcactca
     2101 tgtggttcat tcgctccgcc agcactccga attcgtcgcg attggagacc tcgatgcgcg
     2161 cgccgaaatc gccggccgcc acctcgtcga gaaagccttg cgcctcgcgt accgggagga
     2221 tgaacgacca ggatatgacg aagccgcaga acagggcgaa gattaccgcg atcaccgcga
     2281 acgcgctggt cagaatcagc gagcgcttgt tggcggcggc gacgctgtcg cgcaaccggg
     2341 tcatccggtc ctgctgcgcc gccaccaaga gtccgacccg cgtggtgatc tcggtatcga
     2401 gccgctcctg gcggtgcaac agcgggccag taactgcgcc aagcttgccg tcgcgaatcg
     2461 cattggctat gtcggcgacg acggccatcg cctcgtcctg cgaggtcctt atctgctcga
     2521 ccagtgtccg gtcggcggtg ccggctgatt ccagcttcgc cagccggtcg ttgaactggt
     2581 tgttctcgcg caggatccgg gcgatcgccg cctcgtcctg cgaaaggagc gcgacatcgg
     2641 tgtagtgcat ctgcctcgcc agcgcgcgtt cgctttgctg cgcgaggctc accagctcgt
     2701 gcgcttcgtc gagctggcgg ctctgctgcg tggcattgac cagcgtctgc aggctgaaca
     2761 gggacacggc gatgaacagc agcgtggcga tcaggaacgc ggcgagcagc ttggtgtgca
     2821 ccgaagcgcg gatgccggcg acccagacca ccagcctgtg caacggcgta cgtctctgca
     2881 ccaagtccgc cagatgctcg agcgcgagct ccggacgtgt ggtcgacgac cgattgtcga
     2941 gcgaggactg gatgccatgc cccatctctt tcgttcccgc ttcgtccttg tgcatcggcg
     3001 gccgagaccg tatatttgcc tcgaaccgct ggaattccac tggtgagaaa taccccccgc
     3061 atccttatcg tcgacgacaa cccgaccaac gtcaaggtcc ttcagacccg gctggtcgcc
     3121 gaaggttacg aggtcgtgac cgccgctgac ggtgaagagg gacttgccgt cgcgcggcgg
     3181 gacaagcctg atttgatcct gctcgacgtc atgatgccca agctcgacgg cttcgaggtc
     3241 tgccgccggc tgcgggccga tgcttcgttt ccgttcacac cgatcatcat ggtgacggcg
     3301 atggcggact cgaaggacat cgtcgccgga ctcgaagccg gcggcgacga atatctgacc
     3361 aagcctgtgg accacgcatc gctcgccgcg cgcgtgcgct ccatgctgcg catcaagcga
     3421 ctgcacgaca cggccgagga gctcgccgac gaagtcaagc agtggaacac caagctggaa
     3481 gagcgcgtcg ccgcgcaggt gagcgagctc gagcgggtcg gacgcctgcg gcgctttttt
     3541 tcgccgcatc tggccgagct catcgtcagc ggcggcgccg acgatccgct gaaaagccat
     3601 cgccgcgaaa tcactgtcgt cttcctcgac ctgcgcggct tcaccgcatt cgccgaggtc
     3661 gcggagcccg aggaagtcat gggcgtgctg cgcgagtacc accagatcgc cggacgcatg
     3721 gtcctcgagt acgaaggtac gctcgagcat ttcaccggcg acggcatcac gatcctgttc
     3781 aacgatccgg tgccgattcc gaatcccgcc gagcgcgcgg tgcggatggc gctggccgtg
     3841 cgcgagcgcg tcggccgcat ggcgcaggac tggacgaagc gcggctatga cctgagcctc
     3901 ggcatcggga ttgcccaggg ctttgcgacg ataggcgcga tcggcttcga gggacgatgg
     3961 gattacagtg cgatcggcac cgtcaccaat ctggcctcgc gactttgcgg cgaggctccc
     4021 ggcgaccaga tcctgatctc gcaaaaggtc tatgcaggcg tggaagcgct gatcgaggtc
     4081 gagccgcaag aagcgctgac cttgaagggt ttccgtcgtc cggtcatcgc gtatcgtgtg
     4141 cgcggactga aagcctagcc ggtccgcaac atcgaatccc gcgagcagac gggccgctac
     4201 tactcgagtt cgcgtcgttc agctcgtgcg ggtcgccgcg gtaagggccc ggtcgagatc
     4261 gccgaacagg tcgtccacat cctcgatgcc ggtcgacaga cgcagaaggt cggtcggaca
     4321 cggcgagcca gcgccttcga tcgatgcgcg atgctcgatc aggctctcga tgccgccgag
     4381 cgacgtcgcc cgcttccaca agcggacatt ggcggccgcg gcgatcgccg cgggctcgcc
     4441 gtcacgcacg cgaatcgaca gcatgccacc gaaaccatcg cgcatctgcc gcgcggcaat
     4501 cgcgtggccc ggatgcattg gtagcccggg atagagcacc tcggccaccg ccggatgctt
     4561 ctgcagccga cgcgccagct ccatcgcgct cacgcatacc tgcgcgaccc gcagatgcag
     4621 cgtgcgcatg ccgcggatca gcaggtaggc ttccagcggc ccgaggatca tgcccagcat
     4681 cgtgcgaatg cgctttacgc gttcccagaa ttcatcgatg cgagccgtgg ccagcgcgcc
     4741 cgcaacgaca tccgaatggc cgttcagata tttggtcgcc gaa
//
LOCUS       DATANS010000676         3590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_286105, whole genome shotgun sequence.
ACCESSION   DATANS010000676 DATANS010000000
VERSION     DATANS010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286105"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VHT22_10035"
     CDS             <1..426
                     /locus_tag="VHT22_10035"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX3137939.1"
                     /translation="VVENAHWLTDTDFAQAFAIAQAAPGPNLLIVSLIGWKVDGFLGA
                     LVCTVAICAPSSALTFAVAHVWDRFRDAPLRKAIQYGLAPVTVGLVLASGFILARTVD
                     QSAVAVGLTVATVVLALATRLHPLWMLAAGAAVGVLIAV"
     gene            436..1320
                     /locus_tag="VHT22_10040"
     CDS             436..1320
                     /locus_tag="VHT22_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX3137940.1"
                     /translation="MPELPDVAVYVEALERRVVGHRLEEIRLNSPFVLRTVVPPVAEA
                     QGRRVTGVRRLGKRIVIALEGEIFLVLHLMIAGRLRWLARGIRPPARITLATFAFDTG
                     VLAFTEAGTRKRASLHLVQGPAALAAFDRGGAELDDIDAAAFAARLKQENHTLKRALT
                     DPRLFSGIGNAYSDEILHRARLSPLALTHKLSTEETERLFASAKSVLAEWTERLRREA
                     GEDFPEGVTAFRPQMAVHGRFGKPCPDCGAPVQRIVYADNETNYCANCQTRGTVLADR
                     ALSRLLKASWPRSIDELG"
     gene            1396..2271
                     /locus_tag="VHT22_10045"
     CDS             1396..2271
                     /locus_tag="VHT22_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX3137941.1"
                     /translation="MRWQDYRRSDNVEEGSSSGPSMFGGGLRLSGGAIILVVVVSLLL
                     GKNPLEMLALLMGDGGGPVVQTQAPPTATRAPAANDPQTEFVSRVLGDTEDVWGGIFQ
                     KMGTRYQAPKLTLYHGQIASACGFSSAAAGPFYCPGDRKVYLDLSFFQELSQRFGAPG
                     DFARAYVIAHEIGHHVQNQLGLMDAAQQRMASSSDRSRNDMSVRLELQADCLAGVWGH
                     SSMQRNIIDAKDVESGIAAAAAVGDDRIQKQTRGYVVPEGFTHGSSAQRVKWFRTGLD
                     SGDLRQCDTFATRSL"
     gene            2389..2598
                     /locus_tag="VHT22_10050"
     CDS             2389..2598
                     /locus_tag="VHT22_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137942.1"
                     /translation="MKLILALAVGAFVSACSSVPVQVREPQRADNNSLLACDYARMQT
                     IDRVAQQRGVYVEWVHCPQLKRDRT"
     gene            2689..3360
                     /locus_tag="VHT22_10055"
     CDS             2689..3360
                     /locus_tag="VHT22_10055"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137943.1"
                     /translation="METLEFERLLLGRVALDFCFACQLVWFDEHESTQLAPGGIIEVF
                     KAFDAHRAPTRNPLPELLDCPRCNSRLSLTHDLQRTTHFTYYRCDWGHGRLTPFVQFL
                     LEKNFVRPMSGSELATLKARVRSVQCSNCGAPIDLQNDTVCPYCRSPLAILDPEAVNK
                     ALRELSTAEIQRKTIDVDRLADALTMRAPGIGGSNRSQTAAIDAGLAGDLVAAGVTIV
                     AALLS"
BASE COUNT          557 a         1212 c         1198 g          623 t
ORIGIN      
        1 gtcgtcgaga acgcgcactg gctgaccgac accgacttcg cccaggcgtt tgcgatcgcc
       61 caggcggccc ccggtcccaa cctgctgatc gtcagcctga tcggatggaa ggtggacggc
      121 ttcctgggcg cgctggtgtg cacggtcgcg atatgcgcac cgtcttcggc gctgactttt
      181 gccgtcgcgc atgtctggga ccggtttcgc gatgcgccgc tgcgcaaggc tatccagtac
      241 ggcctcgcgc cggtcaccgt cggactggtg ctggccagcg gcttcatact cgcccgcacc
      301 gtcgaccagt cggctgtcgc cgtcgggctg accgtggcga ccgtcgtgct ggcgctcgcg
      361 acgcgcctgc acccgctgtg gatgctggcc gccggcgcgg cggtcggcgt tttgatcgcc
      421 gtctaggccg gcggcatgcc ggagttgccc gacgtcgccg tttacgtcga agcgctcgag
      481 cggcgcgtcg tcgggcaccg gctggaggaa atccgcctga acagtccgtt cgtgcttcgc
      541 acggtggtgc cgccggtcgc ggaggcgcag ggacggcgcg tcaccggcgt gcggcggctg
      601 ggcaagcgca tcgtcatcgc gctcgaaggc gagattttcc tggtcctgca cctgatgatc
      661 gccggccggc tgcgctggct ggcgcgcggg atccggccgc cggcgcgcat cacgctggcg
      721 accttcgcgt tcgacactgg cgtgctggcg ttcaccgaag cgggcacgcg caagcgagcc
      781 tcactgcacc tcgtccaagg cccggccgcg ctggcggcgt tcgaccgcgg cggcgccgag
      841 ctcgacgaca tcgatgccgc cgccttcgct gcgcgcctga agcaggaaaa ccatacgctc
      901 aagcgcgccc tgaccgatcc gcgattgttc agcggcatcg gcaacgctta ctccgacgag
      961 atcctgcatc gcgcccgcct gtcgccgctg gcgctgaccc acaagctcag cacggaggag
     1021 accgagcggc tgttcgcctc ggccaagtcg gtactggccg aatggaccga gcgcctgcgg
     1081 cgcgaagcgg gcgaggattt tccggaaggc gtcaccgcgt tccggcccca gatggcggtg
     1141 cacggccgct tcggcaaacc gtgcccggat tgtggcgcgc cggtgcagcg catcgtctac
     1201 gccgacaacg aaacgaatta ctgcgcgaat tgccagaccc gaggcacggt gctcgccgat
     1261 cgcgcgttgt cccgcttgct gaaagcgagc tggccacgct cgatcgacga gctcggttga
     1321 gccccgcaag cgaggcgcgt agactggccc acgagggttg aacgcgccgc tgcaagtttg
     1381 acaggagagg caaccatgag atggcaggac tatcggcgca gcgacaacgt cgaggaaggc
     1441 tcgagcagcg gtccgagcat gttcggcggc gggcttcgcc tctcgggcgg ggcgatcatt
     1501 ctggtggtcg tcgtcagcct gctgctcggc aagaacccgc tcgagatgct cgccctgctt
     1561 atgggggacg gtggcgggcc cgtcgtgcaa acgcaggcgc cgccgactgc gacgcgtgcg
     1621 ccggcggcga acgatccgca aaccgaattc gtctcgcgcg tgctcggcga caccgaggac
     1681 gtctggggcg gcatcttcca gaagatgggc acgcgctatc aggcgccgaa gctcacgctc
     1741 tatcacggtc aaatcgcctc ggcctgcgga ttcagcagcg cggcggcggg gccgttctac
     1801 tgtcccgggg accgcaaggt atacctcgac ctgagctttt tccaggagtt gtcgcagcgc
     1861 ttcggcgcac ccggcgattt cgcgcgggcg tacgtgatcg cccacgaaat cggccatcac
     1921 gtccagaatc agctcgggct gatggacgcg gctcagcaaa ggatggccag ctcgtcggac
     1981 cgcagcagga acgacatgtc ggtccggctc gagttgcagg ccgactgcct ggctggcgtt
     2041 tggggacatt cgtcgatgca gcgcaacatc atcgacgcca aggatgtcga gtcgggaatc
     2101 gccgcggcgg cggcggtggg cgatgaccgc atccagaagc agacccgcgg gtacgtcgtg
     2161 cccgaaggtt tcacccacgg ttcgtcggcc cagcgggtta agtggttccg gaccggactc
     2221 gactccggcg acctgcggca gtgcgacacc tttgccaccc gcagcctcta ggctcgggtg
     2281 gcggcgccgc gacaacggcc gcccgccgat ggatggcggt tacggatcgt tcgggcttgc
     2341 cccctcccgg gcttttgcct atagtcagaa gtgcaggagg gtcacactat gaaactcatc
     2401 cttgccttgg cagtcggtgc cttcgtcagc gcttgttctt cggttccagt gcaggtgcgg
     2461 gagccgcaga gggccgacaa caattcgctg ctggcttgcg actacgcgag aatgcagacc
     2521 atcgaccgcg tcgctcagca gcggggggtc tacgtcgagt gggtccactg cccgcagctc
     2581 aaacgcgatc gcacctagtt cggaccgagg tctgagcgcg catctgcggt atcggcggaa
     2641 gtctccgcgg gcgcgcgccg cgtgaactgc cccggttgcg gggccctgat ggagacgctc
     2701 gagttcgagc gtctgctgct cggtcgggtt gcactcgatt tctgcttcgc ctgccagctc
     2761 gtctggttcg atgagcacga gagcacgcaa ctcgcgcccg gcggcatcat cgaagtcttc
     2821 aaggcattcg acgcgcatcg cgcgccgaca cgtaatcccc tgcccgagtt gctcgactgc
     2881 ccgcgctgca attcgcggct ctctctgacg cacgatcttc aacgcacgac ccatttcacc
     2941 tattaccgct gcgattgggg tcacggccgc ctgacgccgt ttgtgcagtt cctgctggaa
     3001 aagaacttcg tgcgaccgat gtccggaagc gagctcgcaa cgctcaaagc gcgggttcgc
     3061 agcgtccagt gctcgaactg cggcgcgccg atcgatcttc agaacgatac cgtgtgtccg
     3121 tactgccgct cgccgctggc gatcctcgat cccgaagcgg tcaacaaagc gctgcgcgag
     3181 ctctcgaccg ccgagatcca gcgcaaaacc atcgacgtcg atcgcctcgc cgacgccctg
     3241 acgatgcgcg cgccgggcat tggcggctcg aatcgcagcc agactgcagc catcgacgcc
     3301 gggctggccg gcgatctggt cgccgcaggc gtgaccatcg tcgccgcgct gctgagctga
     3361 cacgtcattc cgccgcggcg ccgagccgga tttcttccca ggcggtgcgc gccggcataa
     3421 aatctcgttt tgtcttcaag ccacgaggtc ctgaatgcac ttccgcctga tttccgccgc
     3481 gtcgatcgtg gcgttctcgc taggcgccgc ggcacaaacg ccgccgccgg cggccgccgg
     3541 cgcgcctctg gtcgcgccca atacctgtgt caggcccgag ttttcggggc
//
LOCUS       DATANS010000677         5608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_117689, whole genome shotgun sequence.
ACCESSION   DATANS010000677 DATANS010000000
VERSION     DATANS010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117689"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2826
                     /locus_tag="VHT22_10060"
     CDS             <1..2826
                     /locus_tag="VHT22_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3400 domain-containing protein"
                     /protein_id="HEX3137944.1"
                     /translation="ASFELAYHAATADGGVGAPIRTETLSIPGERFRKTGLGKDVTDK
                     FLAGLPGVQKEGCDGIITSARFILHTMPPAIRTVCLEFFGQVRESTPAIVEIKRYIDT
                     LAGAAPGERVQLAGLEHLDERYVKAVGYATKAKRHGRPKMVLIGDIVGDSDNAVARAA
                     SEVVRICNARGAEGFVAVSAEARKKFWLDRARTAAIARHTNAFKINEDVVIPLERLGD
                     YTDGIERINIELSIGNKLVLCDALSAFFAGPLPAALWSADADAAPAQDALDAKVDEAR
                     VLIGAVRARWQDLLDRLDETFPALQSRAELVSWKAEIKAPLQEIFAGAAFAPILKCCA
                     DIHRSVLHGRVFIALHMHAGDGNVHTNIPVNSDDYAMLQQANASVGRIMALARALGGV
                     ISGEHGIGITKLEYLTADEIAPFIAYKQKVDPGGHFNRAKLLPGADLSGAYTPSFSLL
                     GAESLILEQSEIGRIADSIKDCLRCGKCKQPCATHVPRANLLYSPRNKILATSMLIEA
                     FLYEEQTRRGVSLKHFDEFGDVADHCTVCHKCLNPCPVDIDFGDVSIAMRNLLRRQGH
                     AKFNPGTTAAMFFLNATDPTRIKLTRKLMMVWGYRLQRLAYRVVKWMGLAAAQTGRPP
                     ATVGGTPLVTQVIHFVNKPMPGGLPKRSARALLDIEDSTVVPIIRDPSRAAEESDAVF
                     YFPGCGSERLFSQVGLATQAMLYHVGAQCVLPPGYLCCGYPQTSAGNDDKGREIITDN
                     RVLFHRVANTLNYLDIKTVLVSCGTCMDQLQKYEFEKIFPGCRLLDIHEYLLEKGVRL
                     GEGELPGVRYMYHDPCHTPMKTYQPLKVVNQLMGTKVALNDRCCGESGTLASTRPDIA
                     TQVRFRKEEEMRKGASALRGDGFDGKVKVLTSCPSCLQGLSRFADDAGTDADYIVVEI
                     ARRVLGPDWLPDYVKAANNGGIERVLV"
     gene            complement(2873..3250)
                     /locus_tag="VHT22_10065"
     CDS             complement(2873..3250)
                     /locus_tag="VHT22_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137945.1"
                     /translation="MYTDFAYNLPHPLNVRESLARGGYENRELRAAGRRASETARRDA
                     PSRRYRLPPTRAARSSRSRGVFFAPGKRLLSAVVIAAALFTMAAMFGTATVVDSATFN
                     ANAGPRLVASASLAPAPILALTR"
     gene            3469..5091
                     /locus_tag="VHT22_10070"
     CDS             3469..5091
                     /locus_tag="VHT22_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137946.1"
                     /translation="MVTNPTTPAQAQGHPPLGQLAFADADGCATWLRNLPVTNIPRYY
                     EEILDQLRRVGEAEFAPRERGRIAELMREPVSFLHTELARRYAGKPQPAAAREKEASD
                     QALALWQALWVQYSACLKPLLEGDAELQGVKAKVLQRGLWVGKQLVLVHGLARRTPGA
                     SVWQELHAYFRLAEILECAVTAVSDDLMPNAIGLSCYSTYAHALLLDLADPCSMTVRQ
                     IELTDRWLAMWARKIFPYAQQRETEGPVITVDLDGLGGATLHAAEPRHGSQSMRYCYP
                     AKLTSSVRGRLKRLASGANPAELQLGHDVSAEACSTLLAQLDAHWYALPLKRGEGGES
                     QTGLELCAGGLGAAYFRVSGKTFNSKDLVGHLSYQGTQHLATLGALTDYDRNKDEAER
                     SWAWERWQGNYEWTAAMLHRPAVGQYRWHLDQLVVIRDDERLRCGHVTHVALDSRDEL
                     TLGIKLWPGAPTAIAVRALTTMLVEEAPIPTVILGATPDEKPAMLFPPRTFTAGRIFR
                     TLSSGPERRFRLTRLLQRGADFERVAFDEIKP"
     gene            5125..>5608
                     /locus_tag="VHT22_10075"
     CDS             5125..>5608
                     /locus_tag="VHT22_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX3137947.1"
                     /translation="MPNRLAGETSPYLQQHASNPVDWYPWGEEALARAKRENKPILLS
                     IGYSACHWCHVMAHESFEDEAVAALMNDLFVNVKVDREERPDLDQIYQAAHAMLSRRT
                     GGWPLTMFLTPQQEPFFGGTYFPKTSRYGLTGFADLLPQLAQAYREQGEAIAEQNERL
                     K"
BASE COUNT          993 a         1817 c         1865 g          933 t
ORIGIN      
        1 gcaagcttcg agctcgcgta ccatgcggcg acggcggacg gcggcgtcgg cgcgccgatc
       61 cgcaccgaga cgctgtcaat cccgggcgag cggttccgca agaccgggct cggcaaggac
      121 gtgaccgaca agtttctcgc agggctcccc ggcgtgcaga aggaaggctg cgacggcatc
      181 attacgtcgg cgcgcttcat cctgcacacg atgccgccgg cgattcgcac cgtgtgcctg
      241 gaattcttcg gtcaggttcg cgagtcgacg ccggcgatcg tcgagatcaa gcgctatatc
      301 gacacgctcg ccggcgcggc accgggcgag cgcgttcagc tcgccgggct cgagcatctc
      361 gacgagcgct acgtcaaggc ggtcggctac gcgaccaagg cgaagcgaca cggccggccg
      421 aagatggtgc tgatcggcga catcgttggc gacagcgaca atgcggtcgc gcgcgcagcc
      481 tccgaggtcg tacgcatctg caacgcccgc ggcgccgaag gctttgtcgc ggtctcggcc
      541 gaggcgcgca agaagttctg gctcgaccgc gcccgcacgg cggcgatcgc ccggcacacc
      601 aatgcgttca agatcaacga ggacgtcgtc atcccgctcg agcgtctcgg cgactacacc
      661 gacggaatcg agcgcatcaa catcgaactg tcgatcggca acaagctcgt gctgtgcgat
      721 gcgctttcgg cgttcttcgc agggccgctg ccggcggcgc tttggagcgc cgacgccgac
      781 gccgcaccgg cgcaggatgc actggatgcc aaggtcgacg aggcgcgcgt gctaatcggg
      841 gctgtgcgcg ctcgatggca ggacctcctc gaccggctcg acgagacctt tcccgcgctg
      901 caaagccgcg ccgagctcgt gtcctggaaa gcggagatca aggctccgct gcaggaaatc
      961 ttcgccggcg ccgcgttcgc tccgatactg aagtgctgcg ccgacattca tcgttcagtg
     1021 ctgcacggac gagtattcat tgcgctgcac atgcatgccg gcgacggcaa cgtccacacc
     1081 aatatcccgg tgaactcaga cgactatgcg atgctgcagc aggccaatgc gtcggtgggg
     1141 cgcatcatgg cgctggcgcg cgccctgggc ggcgtcatct ccggcgagca cggcatcggc
     1201 atcaccaagc tcgaatacct gacggcggac gagatcgcgc cgtttatcgc gtacaagcag
     1261 aaggtcgatc cgggtgggca tttcaaccga gccaaactgt tgcccggcgc cgatctgtcc
     1321 ggagcctaca ccccgagctt cagcctgctc ggcgccgaga gcctgatcct cgaacagtcg
     1381 gagatcggac gaattgccga ctcgatcaag gattgcctgc gttgcggcaa gtgcaaacag
     1441 ccgtgcgcaa cgcacgtgcc gcgcgccaac ctgctgtaca gcccgcgcaa caagatcctg
     1501 gcgacctcga tgctgatcga ggccttcctg tacgaggagc agacgcggcg cggcgtgtcg
     1561 ctgaaacatt tcgacgagtt cggcgacgtc gccgaccact gcacggtctg ccacaagtgc
     1621 ctgaatccgt gcccggtcga catcgatttc ggcgacgtct cgatcgcgat gcgcaacctg
     1681 ctgcgccgcc agggtcacgc caaattcaat ccgggaacga cagcggcgat gttctttttg
     1741 aacgcgaccg acccgacgcg gatcaagctg acgcggaagc tgatgatggt gtggggctac
     1801 cggctgcagc gtctggcata ccgcgtggtg aagtggatgg gcctcgcggc ggcgcagacc
     1861 gggcggccgc cggcaaccgt cggcgggacg ccgctggtga cgcaggtgat ccacttcgtc
     1921 aataagccga tgccgggcgg cttgccgaag cgctcggccc gggcgctgct cgatatcgag
     1981 gattcgaccg tcgtgccgat catccgcgac ccgtcgcggg ccgccgagga atcggacgct
     2041 gttttctact tccccggctg cgggtccgag cgccttttct cgcaggtggg actcgccacg
     2101 caggcgatgc tctatcacgt cggcgcgcaa tgcgtgcttc ctcccggcta cctatgctgc
     2161 ggctatccgc agacatcggc aggcaacgac gacaagggcc gggagatcat caccgacaac
     2221 cgcgtgctgt ttcatcgcgt cgccaacacg ctcaactacc tcgacatcaa gacagtgctg
     2281 gtttcgtgcg ggacttgcat ggaccagctg cagaaatacg agttcgaaaa gatttttccc
     2341 ggctgccggc tgctcgacat ccacgaatat ctgctggaaa aaggcgtgag gctcggcgaa
     2401 ggcgagctcc ccggcgtgcg ctatatgtac cacgacccct gccatacgcc gatgaagacc
     2461 taccagccgc tgaaagtggt caaccagctg atgggaacga aagtggcgct gaacgaccgc
     2521 tgctgcggcg aatcggggac actggcgtcg acgcggccgg atatcgccac ccaggtccgt
     2581 tttcgcaagg aagaggagat gcgcaagggc gcctctgcgc tccgcggcga cggcttcgac
     2641 ggcaaggtga aggttctgac ctcgtgtccg tcgtgcctgc aggggctgtc ccgattcgcc
     2701 gacgatgccg gcactgacgc cgactacatc gtcgtcgaga tcgcgcgccg ggtgctcgga
     2761 ccggattggc tgccggatta cgtcaaggcc gcgaacaacg gcggcatcga gcgcgtgctg
     2821 gtgtaggtgc cggccacatt cggtgaccgt cgctgtcgcc gaaggcgcgc gttcagcgcg
     2881 taagagcgag gatcggcgcc ggtgcgagcg acgccgaggc gaccaggcgt ggccctgcgt
     2941 tggcgttgaa agtggcgctg tcgacgaccg tcgccgtgcc aaacatcgcc gccatcgtga
     3001 acaatgccgc tgcgatcacg accgccgaga gcaagcgctt gcccggcgcg aaaaagaccc
     3061 cgcgactgcg cgaggatcgc gcagcgcggg ttggaggcaa gcggtagcgg cgcgacggtg
     3121 cgtcgcggcg agcggtttcc gacgctcgac ggccggccgc gcggagttcg cgattttcat
     3181 atccgccgcg cgccagcgat tcgcgcacgt tcagcggatg gggcaggttg tatgcgaagt
     3241 cggtgtacat atgggtctcc agatcgagtc ggcggcggat ggggttatcg gtcgctggcc
     3301 ctttctctgt tgtacacagt ctggtcgtct cagcgacccg gcgcgccggc agattgcgca
     3361 ccggaaaacc cgactattca cgatgtcgcg ccctcccggc gccgtgcggc ggccggctat
     3421 ccgcccggca tggcagttgt gctataaaga ctcgaatatg gcgctgtcat ggtgaccaac
     3481 ccaaccacgc ctgcgcaggc gcagggccac ccccccctcg gccagctcgc tttcgccgac
     3541 gccgacggct gcgcgacgtg gctgcgcaac ctgccggtca ccaatatccc gcggtattac
     3601 gaagagatac tcgaccagct gcggcgggtc ggcgaggcag agtttgcgcc gcgcgagcgc
     3661 ggccggattg ccgagctgat gcgcgagccg gtgagcttcc tgcataccga gctcgcacga
     3721 cgttatgcag gcaagccgca gccggctgca gcgcgcgaaa aggaagcgtc cgaccaggcg
     3781 cttgcgctct ggcaggcgtt atgggtccag tactcggcat gtcttaaacc gctgctcgaa
     3841 ggcgacgccg agctgcaggg cgtcaaagcc aaggtcctgc agcgcggcct ttgggtcggc
     3901 aaacaattgg tgctggtgca cggcctggcg cgccgcacac cgggcgcgtc ggtctggcag
     3961 gagctgcatg cctatttcag gttggccgaa atcctcgagt gcgccgttac ggcggtgtcg
     4021 gacgatctga tgcccaatgc catcggactg tcctgttact cgacttacgc gcatgcgctg
     4081 ttgctcgatc tggccgatcc gtgcagcatg acagtgcgtc agatcgaatt gaccgaccgc
     4141 tggctggcga tgtgggcgcg caagatcttt ccgtatgcgc agcagcgcga aaccgaggga
     4201 ccggtgatca cggtcgatct cgacggtctc ggcggcgcga cgctgcatgc cgccgagccg
     4261 cgccacggca gccagtcgat gcgttattgc tatccggcga agctcacgtc gagcgtgcgc
     4321 ggacgattga agcgcctcgc cagcggagct aatcccgccg agctgcagct ggggcacgat
     4381 gtctccgccg aggcgtgctc gacgctgctg gcgcagctcg atgcgcactg gtacgcgctg
     4441 ccgctcaagc gcggcgaggg cggtgaatcg caaacggggc tggagctttg cgccggcggc
     4501 ctcggcgccg cgtactttcg ggtgtccggc aagaccttca acagcaagga cctggtcggc
     4561 cacctgtcgt accagggaac gcagcacctg gccacgctgg gcgcccttac cgactacgat
     4621 cgcaacaaag acgaagccga gcgctcgtgg gcgtgggaac gctggcaggg aaattacgag
     4681 tggacggccg cgatgctgca ccgcccggcc gtcggccagt accgctggca tctcgatcag
     4741 ctggtcgtga ttcgcgacga cgagcgcttg cgctgcggac atgtcacgca tgtcgcgctc
     4801 gacagccgcg atgagctgac gctggggatc aagctttggc caggggcgcc gacggcgatc
     4861 gcggtgcgag cgctgacgac gatgttggtc gaagaggccc cgataccgac ggtgatcctc
     4921 ggcgcgacac ccgatgaaaa gcccgcgatg ctcttcccgc cacgcacgtt cactgccggc
     4981 cgcattttcc gcaccctctc ctccgggccc gagcgccgct tccgcctgac tcgccttttg
     5041 cagcgcggcg ccgatttcga gcgcgtcgcg ttcgacgaaa tcaagcccta gctcgcgggc
     5101 cggcttccgg tccttgacgt cgacatgccc aatcgtctgg cgggcgagac cagcccctat
     5161 ctgcagcagc acgcgagcaa cccggtcgac tggtatccgt ggggcgaaga agcgctcgcg
     5221 cgggcgaagc gtgagaacaa gccgatcctg ctgtcgatcg gatattcggc atgccattgg
     5281 tgccacgtga tggcgcacga atcgttcgaa gacgaggccg tcgcggcgct gatgaacgac
     5341 ctgttcgtca acgtcaaggt cgatcgggaa gagcggccgg atctggacca gatctaccag
     5401 gccgcgcatg caatgctctc gcgaaggacc ggcggctggc cgttgacgat gttcctgacg
     5461 ccgcagcagg agcccttttt cggcggcacc tatttcccga agacctcgcg ttacgggctg
     5521 accgggttcg ccgacctgct gccgcagctc gcgcaggcgt atcgcgaaca gggagaggcg
     5581 atcgccgagc agaacgagcg gctcaagg
//
LOCUS       DATANS010000678         6669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_202530, whole genome shotgun sequence.
ACCESSION   DATANS010000678 DATANS010000000
VERSION     DATANS010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202530"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /gene="purD"
                     /locus_tag="VHT22_10080"
     CDS             complement(<1..1235)
                     /gene="purD"
                     /locus_tag="VHT22_10080"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX3137948.1"
                     /translation="MKLLVIGGGGREHALAWKIAQSQRVSQVFVAPGNAGTSREPGVI
                     NIAQQEIPALVEFARRESIALSVVGPEAPLAAGLVDAFRAAGLRIFGPTQAAAQLESS
                     KDFAKDFMVRHRIPTARHRTFDDPSEAHAYIDARGAPIVVKADGLAAGKGVVVAATAD
                     EAHAAVDAMLVEYAIPVQHAAGGARVVIEDLLEGEEASFIVMADGKHVLALASSQDHK
                     RLSDGDRGPNTGGMGAYSPAPVVTPEVHARIMREIILPAVNGMASDGIPYTGFLYAGV
                     MIDAAGNPSTLEFNCRLGDPETQPIMMRLKSDLVDLFEHGINGTLDRIDAEWDRRVAL
                     GVVVAAAGYPGDPRRGDVISGLDQASDECKVFHAATSIADGKVVTSGGRVLCVTALGD
                     NVRHAQRNAYAAVALIHFDG"
     gene            complement(1290..2858)
                     /gene="purH"
                     /locus_tag="VHT22_10085"
     CDS             complement(1290..2858)
                     /gene="purH"
                     /locus_tag="VHT22_10085"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX3137949.1"
                     /translation="MNTPIARALLSVSDKTGLVEFARGLANLSVALLSTGGTAKALAA
                     AGLDVTEIDAYTGFPEMLDGRVKTLHPKVHAGILARRDSSEHAATLAAHGIPPIDLVV
                     VNLYPFRETVAKAGCTLDEAIENIDIGGPTLVRAAAKNHAGVGVVVDPDDYAALLAEL
                     KANAGRLSAATRFRLAQKAFSHTAAYDGAISNYLTARDDAGTARLFPDRFNWQGAKLQ
                     DLRYGENPHQQAAFYRDETPAGGTIAAARQLQGKELSYNNIADSDAAWECVKTFGETA
                     CVIVKHANPCGVAVAATPLQAYRKALATDPTSAFGGIIAFNREVDAETADAVGAQFVE
                     VLIAPSYTAEALERNAKKANVRVLKLALAMVPQRSWDSKRVGGGMLIQTPDVDNLAAG
                     ELKVVTKKAPTAAQLTDLLFAWRVARFVKSNAIVYCAGGATLGIGAGQMSRVDSTRIA
                     AVKAASAGLSLAGSVVASDAFFPFRDGLDVVADNGAIAVIQPGGSVRDAEVIAAADER
                     GIAMVFTGIRHFRH"
     gene            complement(2950..3186)
                     /locus_tag="VHT22_10090"
     CDS             complement(2950..3186)
                     /locus_tag="VHT22_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3137950.1"
                     /translation="MNGGNEICRSVEKSLEEYFRKLDGEQPHSIYDMVIANVERSLLA
                     SVMHRAGGNQTQAADMLGLNRNTLRSKLSKYNIR"
     gene            complement(3201..4241)
                     /gene="dusB"
                     /locus_tag="VHT22_10095"
     CDS             complement(3201..4241)
                     /gene="dusB"
                     /locus_tag="VHT22_10095"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX3137951.1"
                     /translation="MQIGPHHISIPLVVAPMAGVTDRPFRQLCKRLGAGLAVSEMVAS
                     NPKLWNTAKSLRRTDHRGEVEPIVVQIAGADPVMMADAARYNVERGAQIIDINMGCPA
                     KKVCNVAAGSALLQDEPLVAAIVAAVVAAVAVPVTLKIRTGWNRQNRNAVRIARIAED
                     AGIAALSVHGRTRECAFVGPVEYETIRSIKREVGIPVIANGDIDTPEKAREVLARTGA
                     DAIMIGRAAQGRPWIFREIGHFLATGERLPPPRVDEMHRLIIEHLADHYAFYGESDGV
                     RIARKHLGWYTGKLAGGDAFRAQMNRLESSADQVAAVDRYFKRLAAVGERLVFASDAQ
                     IEASWVEEALAA"
     gene            complement(4417..5574)
                     /locus_tag="VHT22_10100"
     CDS             complement(4417..5574)
                     /locus_tag="VHT22_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX3137952.1"
                     /translation="MNDVVIVGAGPVGATLALALAGGDLDVVSLDARARGTLASGDRS
                     LALSHGARLIFDRLGVWSSVAGTADAVTAITAIDVSQRGGFGAARLDAQEHGVPALGY
                     VVSYRALQAALDAGLARAGLPVDHGVAVSKVDATPAYARIRGEREGAACEWTARLAAV
                     ADGGGDIVAGNVRHRHDYKQVALVAKLWRNEPHRGVAYERFTPEGPMALLPEGDHYGL
                     VWTTTPAQGRSLAAMPEAEFLGALATRFGARVGGFLKSEDRRTFPLSLEFSGTVVGAR
                     TVLLGNAAQALHPVAGQGFNLGVRDAYELAQVLLSTPRDRLGATTSLSAYARRRLGDR
                     WAGIALTHGLLEVFGSDAALLRWPRGLALTLLDALPPLKRIFTHAMLYGLH"
     gene            complement(5638..>6669)
                     /locus_tag="VHT22_10105"
     CDS             complement(5638..>6669)
                     /locus_tag="VHT22_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEX3137953.1"
                     /translation="PDAAREIFGFDEAYSIGELQTRLTDWSGDRPAVFTPIGGYPAWD
                     DLVTTTLNAVRNRVRTGIAAPEVVVDVRAALSAMRLTKDEQELKLMRRAAEISCGAHR
                     RAMARTRADWYEYQVEAELMHEFLRSGAQAVAYPSIVASGPNACVLHYRDNNRQMQSG
                     DLLLIDAGCEYEGYASDVTRTFPVGGKFSGPQKDVYELVLAAQLACIDAVAPGRPFHA
                     YHEVAERVLAQGLIDLKLCEGSLDAVLESGAYKQFYMHRAGHWLGLDVHDVGLYRVNG
                     ESRLLEPGMLLTVEPGCYIRPGDKVPEEFWDIGVRIEDDVLVSATGNENLTAAAPKTV
                     ADIEALMQH"
BASE COUNT         1106 a         2254 c         2196 g         1113 t
ORIGIN      
        1 ccgtcgaaat gaatgagagc gacggcggcg taggcattgc gttgggcgtg ccgcacgttg
       61 tcccccagcg cggtgacgca aagcacgcgc ccgccgctgg tcacgacctt gccgtcggca
      121 atcgaagtgg cggcatgaaa caccttgcac tcgtcgctcg cctgatcgag accggagatg
      181 acatcgccgc ggcgcgggtc gccgggatag ccggcggcgg cgacgacgac gccgagcgcg
      241 actcgccggt cccactccgc gtcgatccgg tcgagcgtgc cgttgattcc gtgctcgaag
      301 agatcgacca ggtccgattt gagccgcatc atgatcggct gggtttccgg gtctcccaaa
      361 cggcagttga attcgagcgt cgatggattg ccggcggcgt cgatcatcac gccggcgtag
      421 aggaatccgg tgtacggtat accgtcggaa gccatgccgt tgaccgccgg cagaatgatc
      481 tcgcgcatga tcctcgcgtg cacctccggc gtcacgaccg gagccggcga gtacgcgccc
      541 atgcccccgg tattcggtcc gcgatcgccg tcggacaggc gcttgtgatc ctgcgacgaa
      601 gcaagcgcca gcacgtgctt gccgtcggcc atcacgatga agctcgcctc ctcgccttcg
      661 agcagatcct cgatgacgac gcgcgctccg ccggcagcgt gctggaccgg gatggcgtat
      721 tcgaccagca tcgcatcgac cgccgcatgc gcctcgtccg cggtcgcggc gacgacgaca
      781 cctttccccg cagccagccc gtccgccttt acgacgatgg gcgcgccgcg cgcgtcgatg
      841 taggcatgcg cctccgacgg atcgtcgaac gtgcgatgcc gagcggtcgg aatgcggtga
      901 cgaaccatga aatccttggc gaagtccttc gagctttcca gttgcgccgc agcttgtgtc
      961 ggcccgaata tcctcaggcc cgcggcgcga aaggcgtcga ccaggcccgc ggcgagcggc
     1021 gcctccggtc cgacgaccga cagcgcgatc gactcgcggc gcgcgaattc gaccagggcc
     1081 ggaatctcct gctgcgcaat attgatgacg ccgggctcgc ggctggttcc ggcattgccg
     1141 ggcgcgacaa agacctgcga cacgcgttgc gactgggcga tcttccaggc caacgcgtgc
     1201 tcgcggccgc cgccgccgat gaccagcagc ttcatcgtgc ccgcgccggc tgcgccgcca
     1261 tcgcgatgcg catcgctgga ttcgagcggc taatggcgaa aatgacggat gccggtaaaa
     1321 accatcgcga tgccgcgctc gtcggccgca gcgatcactt ccgcgtcgcg cacgctgccg
     1381 cccggctgga tgaccgcgat cgccccgttg tcggcgacca cgtccaggcc gtcgcgaaac
     1441 gggaaaaacg catccgacgc gaccaccgat ccggcgagcg acaggccggc gctggccgcc
     1501 ttgaccgcgg cgatgcgcgt cgaatcgacg cggctcatct gcccggcgcc gattccgagc
     1561 gtcgcgccgc cggcgcaata gacgatggcg ttggacttga cgaatcgggc gacgcgccag
     1621 gcgaagagca ggtcggtcag ttgcgcggcg gtcggcgcct ttttagtgac gaccttgagc
     1681 tcgcccgccg ccaggttatc gacgtccggc gtctggatca gcatgccgcc gccgacccgc
     1741 ttcgagtccc agctgcgctg cggaaccatc gccagcgcaa gcttgagcac gcgtacattg
     1801 gcctttttcg cgttgcgctc gagtgcttcg gcggtatagg agggcgcgat cagcacttcg
     1861 acgaattgcg cgccgaccgc atcggcggtt tcggcgtcga cttcgcggtt gaacgcgata
     1921 atgccgccga aggccgaggt cgggtcggtc gcaagcgcct tccgatatgc ctgcagcggc
     1981 gtcgccgcca ccgcgacacc gcacggattg gcatgcttga cgatcacgca ggccgtctcc
     2041 ccgaatgtct tgacgcattc ccaggcggcg tcgctgtcgg cgatgttgtt gtatgagagt
     2101 tccttgccct gcaactggcg agcagccgcg atcgttcctc ccgcgggcgt ctcgtcacgg
     2161 taaaaggcag cctgctggtg cggattttcg ccgtagcgca ggtcctgaag cttcgcaccc
     2221 tgccagttga agcgatccgg aaacaggcgc gccgtaccgg cgtcgtcgcg cgcggtcaga
     2281 taattgctga tcgcgccgtc gtacgcggcg gtatgcgaaa acgccttttg cgcgaggcga
     2341 aagcgcgtcg cggcggagag gcgtccggcg ttggctttca gctcggccag caatgctgca
     2401 tagtcgtccg gatcgacgac gacgccgacg ccggcatggt tttttgccgc cgcgcggacg
     2461 agcgtcgggc cgccgatatc gatgttctcg atcgcctcgt ccagcgtgca ccctgccttg
     2521 gcgaccgtct cgcggaatgg atacagattg acgacgacca gatcgatcgg ggggatcccg
     2581 tgcgcggcga gtgtagcggc gtgttcgctc gaatcgcgcc tggcgaggat tccggcatgc
     2641 actttcggat gcagtgtctt gacccgcccg tccagcattt ccggaaagcc ggtgtaggca
     2701 tcgatctcgg tgacgtcgag tcctgcggcg gcgagcgcct tggccgtgcc gccggtagag
     2761 agcagcgcga cgctcaagtt tgcgaggccc cgtgcaaact cgacgagacc tgtcttgtcg
     2821 gagaccgaaa gcagcgcccg ggcgatcggc gtgttcatgg cgagcggcgg accggtggat
     2881 tgggcgcgac acgcgcgccg cgaagcagca acgggcccgg cgccgtcttc agcacgcgag
     2941 aaccgggccc taccgtatat tgtacttcga gagcttcgag cggagcgtgt tgcgattgag
     3001 ccccagcatg tcggccgcct gcgtctggtt gccgccggcg cgatgcatga ccgaggccag
     3061 cagcgaacgc tcgacattcg cgatcaccat gtcgtagatg ctgtgaggct gttcgccgtc
     3121 gagcttgcgg aaatactctt cgagcgattt ttcgacgctg cggcaaattt cgttgccgcc
     3181 gttcatgcga gtggttcgtt tcacgctgcg agggcctcct ccacccacga agcttctatc
     3241 tgcgcgtctg acgcaaagac gaggcgttcc ccaacggccg cgagccgctt gaaataccgg
     3301 tcgacggcgg caacctggtc tgccgagctt tccagccggt tcatttgagc gcggaacgca
     3361 tcgccgccgg cgagcttgcc tgtataccag ccgagatgct tgcgcgcaat gcgcacaccg
     3421 tcgctttcgc cgtagaacgc gtagtggtcg gcaaggtgct cgatgatcag ccgatgcatt
     3481 tcgtcgaccc gtggcggcgg caatcgctcg ccggtcgcga ggaaatgacc gatctcgcgg
     3541 aagatccacg gccggccctg cgccgcccgc ccgatcatga tcgcgtccgc gccggtgcgg
     3601 gccaggacct cgcgcgcctt ctccggcgtg tctatatcgc cgttggcgat caccggtatg
     3661 ccgacctcgc gcttgatcga acggatcgtc tcgtactcga cgggcccgac aaacgcgcat
     3721 tcccgcgtcc ggccgtggac cgacagcgcg gcgatgccgg cgtcctcggc gatccgggca
     3781 atgcggaccg cgttcctgtt ctgccggttc cagccggtgc ggatcttgag cgtcaccggc
     3841 acggccaccg cggccacgac ggcagcgacg atcgcagcga cgagcggctc gtcctgcagc
     3901 aacgccgagc cggccgcgac attgcatacc ttctttgccg ggcagcccat attgatgtcg
     3961 ataatctgcg cgccgcgctc aacgttgtag cgggccgcgt cggccatcat caccggatcc
     4021 gccccggcga tctgcacgac gatgggctcc acttcgccgc ggtggtcggt gcgccgcagc
     4081 gacttcgctg tgttccacag cttgggattc gaggcgacca tttcggacac cgcgaggccc
     4141 gcgccgaggc gcttgcacag ctggcggaac ggtcggtcgg tgacgcccgc catcggcgcg
     4201 acgaccaagg gaatcgaaat gtgatgcggg ccgatctgca cgggaaatag ttgctggacg
     4261 ctgcggcggg gaacggatta taccgagttt ggcgcgcgat cgtcaccgac aaaaaattat
     4321 tgctggcatg ctgcgtgata agtacgcttt cggtcatcgc gccgttcacc gcgcgggaac
     4381 cattcttgct tgatgtcgga cgtgcttgtt ttcgcgtcag tgcaatccgt acagcattgc
     4441 gtgcgtgaaa attcgcttca gcggcggcag cgcgtcgagc agagtaagcg ccaacccgcg
     4501 cggccagcga agcagcgccg cgtcgctgcc gaatacttcg agcaagccat gcgtcaatgc
     4561 gataccggcc cagcggtcgc cgagtcggcg acgcgcgtac gccgacagac tggtggtcgc
     4621 gccgagtcga tcgcgcggcg tcgacagcaa gacctgcgcc agttcgtacg catcgcgcac
     4681 gcccaggttg aaaccctggc cggcaaccgg atgcagcgcc tgcgcggcgt tgcccaggag
     4741 aacggtgcgt gcgccgacga cggtgccgga gaactcgagc gaaagcggaa acgtacgccg
     4801 atcctcgctt ttcagaaacc cgccgacgcg cgcaccgaac cgcgtcgcca gggcaccgag
     4861 gaactcggcc tccggcatcg ccgcaagcga tctgccctga gccggcgtcg tcgtccatac
     4921 cagcccgtaa tgatcgcctt ccggcagcaa ggccatcggc ccctcgggag taaagcgctc
     4981 gtatgccacc ccgcggtgcg gctcgttcct ccaaagcttc gcgaccagcg caacctgctt
     5041 gtagtcgtgt cgatggcgga cattgccggc gacgatatcg ccgccgccat cggcgaccgc
     5101 ggccagtcgc gccgtccatt cgcaggcggc accttcgcgc tcgccgcgga tgcgtgcata
     5161 cgccggcgtc gcgtcgacct tgctgacagc gacgccgtga tcgaccggca ggccggcgcg
     5221 ggcgagcccc gcatcgagcg cggcctgcaa cgcgcgatag ctgacaacgt agccgagcgc
     5281 gggcacgcca tgctcctgcg catccaggcg cgctgcgccg aagccgccgc gctgcgacac
     5341 gtcgatggcg gtaatcgccg tcaccgcgtc cgcggtgccc gcgacggagc tccagacgcc
     5401 gaggcgatcg aaaatcagtc gcgcgccgtg cgacagggcc agcgaacgat cgccggacgc
     5461 cagggtgccg cgcgcacgcg cgtcaaggct gaccacgtcg agatcgccgc cggcgagcgc
     5521 aagcgccagc gtcgcgccga ccggacccgc gccgacgatg acgacgtcgt tcaacacggt
     5581 ttagagcgcc tggtcgagaa cgtctgaagt cacggataga tcggcggcga tccgccgtca
     5641 gtgctgcatc agcgcttcga tgtcggcgac ggtcttcggc gccgccgccg tcaggttttc
     5701 attgccggtg gcgctgacca gcacgtcgtc ctcgatgcgc acgccgatat cccagaattc
     5761 ctcgggtacc ttgtcgcccg gccggatata gcaacccggc tcgacggtaa gcagcatccc
     5821 cggctcgagc aggcgcgact cgccgttgac gcgatacagc ccgacgtcat ggacgtcgag
     5881 accgagccag tgcccggcgc gatgcatata gaactgcttg tatgcaccgg attcgagcac
     5941 ggcgtcgagc gagccctcgc aaagcttcag atcgatcagg ccttgcgcca gcacgcgctc
     6001 ggcgacctcg tggtaggcat gaaacgggcg gcccggggcc acggcgtcga tgcaggcgag
     6061 ctgcgcggcc aggacaagct cgtagacgtc cttctgcgga ccgctgaact tgccgccgac
     6121 cggaaaggtg cgcgtcacgt ccgacgcata accttcgtac tcgcagcccg cgtcgatcag
     6181 cagcaggtcg cccgactgca tctggcggtt gttgtcgcgg taatgcagca cgcaggcgtt
     6241 cgggcccgac gcgacgatcg acggataggc gaccgcctgc gcgcccgagc gcaggaattc
     6301 gtgcatcaat tcggcctcga cctggtactc gtaccagtcg gcgcgcgtgc gcgccatcgc
     6361 acggcgatgc gctccgcagg aaatctcggc ggcccggcgc atcagcttca gctcctgctc
     6421 gtccttggtc aggcgcatcg ccgaaagcgc cgcgcgcacg tcgacaacca cttccggcgc
     6481 cgcgatcccg gtgcgcaccc ggttgcggac tgcgttcaag gtcgtcgtga ccaggtcgtc
     6541 ccaggcaggg tagccgccga tcggcgtaaa aacggcgggc cgatcgccgc tccagtcggt
     6601 gagccgcgtc tggagctcgc cgatcgaata cgcctcgtcg aagccgaaga tttcgcgtgc
     6661 ggcgtccgg
//
LOCUS       DATANS010000679         3373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_74656, whole genome shotgun sequence.
ACCESSION   DATANS010000679 DATANS010000000
VERSION     DATANS010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74656"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VHT22_10110"
     CDS             <1..501
                     /locus_tag="VHT22_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3137954.1"
                     /translation="RVADIQLSFPAILIALILLAVLGQGMLKIIAALVAVQWAYYART
                     VRSAALVEKRREYVEAARCLALTPARIIFRHILPNCLPPLIVVATVQVAAAIALEATL
                     SFLGLGLPITEPSLGLLIANGYQYLLSGKYWISFFPGIALLLTIFSINLVADQLRDVV
                     NPRLQR"
     gene            498..1508
                     /locus_tag="VHT22_10115"
     CDS             498..1508
                     /locus_tag="VHT22_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3137955.1"
                     /translation="MTDAVLSVEGLRTHFITKAGVVKAVDDVSFSVARGQVLGLVGES
                     GSGKSVTGYSIMGLVDPPGRVVAGRIIYRDDNLAALESEAMRQLRGNRIAMIFQDPMM
                     TLNPVLSIETQMVEAILAHERVPYDVAVARARACLVQVGIASPDARLAAYPHQFSGGM
                     RQRVAIAIALLNVPDVIIADEPTTALDVTIQGQILFEMQKLTRETGAALIWITHDLSV
                     IAGLADRVCVMYAGRIVEQGTTADVLGAPRHPYTQGLLDSVPGQAQEHKRLRNIPGMT
                     PSLLDIGPGCAFRARCTYATAVCAQTPEMTPIGPDEHTLRCFHPLPPADLFAAAANEA
                     GR"
     gene            1505..>3373
                     /locus_tag="VHT22_10120"
     CDS             1505..>3373
                     /locus_tag="VHT22_10120"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3137956.1"
                     /translation="MTPEIAGEPAPGPVDRLISRAAGEQLAGRADEAMAILAEALRLD
                     PNHTSALTFAALLALRREQAAAALDYASRAIAVDPRRARAHHLRGLALQAVGRGAEAL
                     VDLRQAVAIDATLVDARLDLGNALLAEGDIASAATEIDAVLAAEPSAIAYNSLGNLRA
                     RQRQFDDAITAYNRAIELDPRLSQAHGNLGMLQFSVADNDAAIASFRRAVALAPDRPA
                     NWSALLFALSVSDRATAGGIAAEHRAFGERFAARIAPLPAVVPAPRAGRRLRIGYVSS
                     DWRRHAVAVFAESLLAAHDRDRFEIFCYQNYAGGDEVTARFAALADHFVPIASMPDAE
                     AARRIRDDAIDILVDLNGHTAHNRLPLFFRKPAPVQATWLGYLGSTGVPTIDYRLTDR
                     YTDPPVQPEVSGAEALWRLPLTQWCYRPYVEAPDVGPLPASTAGGITFVCMNHPLKTS
                     RSAFVLWARILAAVPASRLILLATPYDGQRARVLAFFAGEGIAGERIEQVGEAPIATY
                     LARYQRADVALDSLPCAGGTTTCDALWMGVPVVTMVGDRPFSRSGASLLRNADLTELI
                     ARTPDEYVRAAVDLATDRVRLATLRAGLRDRLRASPLLDPAGFARAVEDAFDKMWER"
BASE COUNT          495 a         1181 c         1118 g          579 t
ORIGIN      
        1 cgcgttgccg acatccagct ttcgtttccg gccatcttga tcgcgctgat cctgctggcc
       61 gtgctcggcc aggggatgct gaagatcatc gccgccctgg tggcggtgca atgggcgtac
      121 tacgcgcgga cggtgcgcag cgccgcgctg gtcgagaagc gcagggagta tgtcgaggcg
      181 gcgcgctgct tggcactgac tcccgcgcgc atcatcttcc gccacatcct gccgaactgc
      241 ctgccgccgc tgatcgtcgt cgcgacggtg caggtcgcgg cggcgattgc gctcgaggcg
      301 acgctgtcct tcctcggcct cggcctgccg atcactgagc cctcgctggg actcctgatc
      361 gccaacggct accagtacct gctctccggc aagtactgga tcagcttctt ccccgggatt
      421 gcgctgctac tgacgatctt cagcatcaac ctggtcgccg atcagttgcg cgacgtcgtc
      481 aatccgaggc tgcagcgatg accgacgcgg tattgagcgt cgaaggcttg cgcacgcatt
      541 tcatcaccaa ggcgggcgtc gtcaaggcgg tcgacgacgt gagcttcagc gtcgcgcgcg
      601 gccaagtgct tggcctggtc ggcgaatcgg gctcgggcaa gtcggtgacc ggatactcga
      661 tcatgggcct ggtcgacccg ccagggcgcg tcgtcgccgg ccggatcatc tatcgcgacg
      721 acaacctcgc cgcgctggag tccgaagcga tgcgccagtt gcgcggcaac cggatcgcga
      781 tgatcttcca ggatccgatg atgacgctga atccggtgct ctccatcgaa acgcagatgg
      841 tcgaagcgat cctcgcccac gagcgcgtgc cgtacgacgt tgccgtggcg cgcgcccgag
      901 cctgcctcgt gcaggtcggc atcgcgtcgc cggatgcgag gctcgccgcc tatccgcatc
      961 aattctccgg cggcatgcgc cagcgggtgg cgatcgcgat cgcgctgctc aatgttcccg
     1021 acgtgatcat cgccgacgag ccgacgactg cgctcgacgt gacgatccag ggccaaatcc
     1081 tgttcgagat gcagaaactc acgcgcgaaa cgggcgccgc gctgatctgg atcacgcacg
     1141 acctttcggt gatcgccggc cttgccgacc gcgtctgcgt gatgtacgcc ggccgaatcg
     1201 tcgaacaagg cacgactgcc gacgtgctcg gcgcgccgcg acacccgtac acgcagggcc
     1261 tgctcgactc ggtgcccggc caggcccagg agcacaagcg gctgcgcaat ataccgggca
     1321 tgacgccgtc gctgctggat atcggccccg gctgcgcgtt tcgcgcacgc tgcacgtacg
     1381 cgaccgcggt ctgcgcgcaa acgccggaga tgacgccgat cggtccggac gagcacacgc
     1441 tgcgctgctt tcacccgctg cccccggctg atctattcgc tgcagccgca aacgaggcgg
     1501 gtcgatgacg ccggagatcg ccggcgagcc agcgccgggt ccggtcgatc ggctgatctc
     1561 gcgtgccgct ggcgagcagc ttgcgggccg cgccgacgag gcgatggcca ttctcgccga
     1621 agcgctgcga ctggatccga atcacacctc ggcgctgact ttcgctgcgc ttctcgctct
     1681 gcgccgcgag caggccgccg cggcgctcga ttatgcatcg cgcgccatcg ccgtcgatcc
     1741 gcgacgggcg cgcgcacacc atctgcgcgg gctcgcgctc caggccgtcg gacgcggcgc
     1801 cgaggccctg gtcgatctgc gacaggcggt cgcgatcgac gccacgcttg tcgacgcacg
     1861 gctggatctc ggcaatgcgc tgctggccga aggcgacatc gcgagcgcgg cgaccgagat
     1921 cgacgcggtg ctggccgccg agccttcggc gatcgcctac aacagcctgg ggaacctgcg
     1981 cgcgcgacag cggcaattcg atgacgccat caccgcctac aaccgggcga tcgagctcga
     2041 cccgcgcctg tcgcaggcgc atggcaatct cggcatgctg cagttcagcg tcgccgacaa
     2101 cgatgccgcg atcgcatcat tccggcgcgc cgtcgcgctc gcgcccgatc gaccggccaa
     2161 ctggagcgca ctgttgttcg cgctcagcgt ctcggatcgc gcgacagccg gtggcatcgc
     2221 tgccgaacat cgcgctttcg gcgagcgctt tgcggcgcgg atcgcgccgt tgcccgccgt
     2281 cgtcccggcg ccgcgcgccg gccggcgcct gcggataggt tacgtttcgt ctgattggcg
     2341 gcggcatgcg gtcgcggtgt ttgccgagtc gctgctggcc gcacacgacc gcgaccgctt
     2401 cgagattttc tgctatcaga attatgccgg cggcgacgaa gtcactgccc gcttcgccgc
     2461 gttggccgac cattttgtcc cgatcgcttc gatgcctgac gccgaggcag cccggcgcat
     2521 tcgcgatgac gcgatcgaca tcctggtcga cttgaacggc catacggcac ataaccgctt
     2581 gccgctgttc tttcgcaagc cggcgccggt ccaggccact tggctcggct atcttggcag
     2641 cacaggcgtg ccgacgatcg actaccgcct gaccgaccgc tacaccgatc cgccggtgca
     2701 gccagaggtc tccggcgccg aagcgctctg gcgactgccg ctgacgcaat ggtgctaccg
     2761 cccttatgtc gaggcgcccg acgtcggtcc gctcccggcg tcgacggccg gcggcatcac
     2821 gtttgtctgc atgaaccacc cgctcaagac ttcgcgcagt gcgttcgttc tctgggcgcg
     2881 catcctggcg gcggtgccgg cgtcacgcct gatactgctg gcaacgcctt atgacggcca
     2941 gcgtgcgcga gtgctggcat tcttcgccgg tgagggcatc gcgggggagc gcatcgagca
     3001 ggtcggcgag gctccgatcg cgacgtatct cgcccgatac cagcgcgccg acgtcgcgct
     3061 cgacagcctg ccatgcgccg gtggtacgac gacctgcgac gcgctgtgga tgggcgtgcc
     3121 ggtcgttacc atggtcggcg atcggccgtt ttcgcgaagc ggtgcgagcc tgctgcgcaa
     3181 cgcggacctg accgagctga tcgcgcgcac gcccgacgag tacgtgcgcg ccgcggtcga
     3241 tctggcgacc gatcgggtgc ggctcgcgac gctgcgcgcg ggactccgtg accggctgcg
     3301 cgcttcgccc ctgctcgacc cggccggctt cgctcgcgcg gtcgaggatg ccttcgacaa
     3361 gatgtgggaa cgc
//
LOCUS       DATANS010000680         1733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_537849, whole genome shotgun sequence.
ACCESSION   DATANS010000680 DATANS010000000
VERSION     DATANS010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537849"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..1342
                     /locus_tag="VHT22_10125"
     CDS             125..1342
                     /locus_tag="VHT22_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX3137957.1"
                     /translation="MAQPRHVIIAGAGIAGLTAALALARAGIRVTVYEQTGKLEETGA
                     GVQLSPNGSRVLIALGLRERLASVAVAPKAIRVMSGGSAREVVRIPLGEEVERRFGAP
                     YWSIHRGDLQSALVDAVRNERDIALELGVRIEDFAAHGRGVSVLGKRGSQVLDERGIA
                     LIGADGIWSNVASRLRRLPPPTFRHRTAWRALVPADAVPEEFRQPLVHLWLGQDAHLV
                     HYPVKSGTLINIVGIVHDEWNEIGWSAAGDRAEILRHFARWAWSEKARALIAVPQRWL
                     KWALYDRKDPFRGSKVPVTLIGDAAHPMMPFLAQGASMAIEDAVVLADMLEKYIDDPA
                     DALRAYEGARWHRTARAQQFSRRQDRIYGRSGPEAFVRNLAMRAMGGERLRARYDWLY
                     SWRPPQRYETVSA"
     gene            1339..>1733
                     /locus_tag="VHT22_10130"
     CDS             1339..>1733
                     /locus_tag="VHT22_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX3137958.1"
                     /translation="MIFPSTVAGSLPKPTWLAEPNKLWPQWRLQGDELAAAKLDATLL
                     AIKWQEDAGIDIVCDGEMSRQHFVHGFLEFVDGIDFAHKVEMGIRGDRYKAMVPVVRG
                     ELKLKGRVHQTEARFARAHTRHKLKFTMPG"
BASE COUNT          307 a          559 c          557 g          310 t
ORIGIN      
        1 catctgcccg tactgttcca cgctctatcg ctttaactca gcgcttgatc cgcatgccgc
       61 gcgcccgcct gagtgcgcgc tgccactttc cgaagccgcc tgacctgcat cgggaatttc
      121 gaccatggcg caaccgcgtc atgtcattat cgccggagcc ggcatcgccg gactgacggc
      181 tgcgctggct cttgcgcgcg ccggcattcg cgtgaccgtc tacgaacaaa ccggaaaact
      241 tgaagagacc ggagccggcg ttcagctttc gccaaacggc agccgcgtcc tgatcgcgct
      301 ggggctacgc gaacggctcg cctccgttgc ggtcgcgcca aaggcgatcc gcgtgatgtc
      361 gggcggatcc gcgcgcgaag tcgtgcgcat cccattgggc gaggaagtgg aacgacgttt
      421 cggtgcgccc tactggtcga tccatcgcgg cgatttgcaa tcggcgctcg tcgacgccgt
      481 tcgcaacgaa cgcgacatcg cgctcgaact cggcgtgcgc atcgaggatt tcgcagcgca
      541 cgggcgaggc gtcagcgtgc tcggcaaacg cggttcgcag gtgctcgatg aacgcgggat
      601 cgccctgatc ggcgccgacg ggatctggtc gaacgtcgca tcacgcctgc gtcggttgcc
      661 gccaccgacg ttccggcacc gcacggcgtg gcgcgcgctc gttccggctg atgccgttcc
      721 ggaggaattt cgccagccgc tggtgcatct gtggctcggc caggacgcgc atctcgtgca
      781 ctatccggtc aagtccggca cgcttatcaa tattgtcggc atcgtgcacg acgaatggaa
      841 tgagatcggc tggtcagcgg ccggcgaccg cgccgaaatt cttcggcatt tcgcccgctg
      901 ggcctggtcg gaaaaagcgc gcgcgctgat cgccgttccg caacgctggt tgaaatgggc
      961 gctctatgac cgcaaggatc cattccgcgg atcgaaggtc ccggtcaccc tgatcgggga
     1021 tgccgcccac ccgatgatgc cgtttctcgc tcagggcgcg agcatggcga tcgaggatgc
     1081 tgtcgtgctc gcggacatgc tggagaagta catcgacgat ccggccgacg cgctgcgggc
     1141 ctatgaaggc gcccgctggc accgcacggc gcgtgcccaa caattctccc gccgtcagga
     1201 tcgtatttat gggcgaagcg gaccggaggc cttcgtgcgt aatctggcga tgcgggccat
     1261 gggcggagaa agactgcgcg cacgctatga ttggctctat agttggcggc caccgcaacg
     1321 ctacgagacc gtttcagcat gatctttcca tcgaccgtcg ccggcagtct gccgaagcca
     1381 acctggctcg ctgagccgaa caagttgtgg ccgcagtggc gactgcaagg cgacgaactc
     1441 gccgccgcca agctcgatgc aacgctgctc gcgatcaagt ggcaggagga tgccggcatc
     1501 gacatcgtct gcgacggaga aatgtcgcgc cagcatttcg tgcacggctt tctcgaattc
     1561 gtcgacggca tcgacttcgc gcacaaagtg gaaatgggaa tccgcggcga ccgctacaaa
     1621 gcgatggtgc cggtcgtgcg cggcgagcta aagctcaaag gccgcgtgca ccagacggag
     1681 gcgcgctttg cccgcgcgca cacccggcac aagctcaagt tcacgatgcc ggg
//
LOCUS       DATANS010000681         3666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_828799, whole genome shotgun sequence.
ACCESSION   DATANS010000681 DATANS010000000
VERSION     DATANS010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828799"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VHT22_10135"
     CDS             <1..445
                     /locus_tag="VHT22_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX3137959.1"
                     /translation="GLIAVTFTLNRWFDLAQRLGAKAGKPGPLIGFLSGATSGLTSTM
                     AHAGGPPLLVYLLPQRMEKTVFVGTSVVFFAIVNYLKLIPYGLLGQLSTNNLGLSLLF
                     APLAPLGIWLGLRLERRIPEKPFYRLSYAILFATGCKLIYDSLTA"
     gene            537..1505
                     /locus_tag="VHT22_10140"
     CDS             537..1505
                     /locus_tag="VHT22_10140"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX3137960.1"
                     /translation="MKQTFLDFEQPIAELQSKIDELRYVHEDSAVDISDEITRLSKKS
                     QQLTKEVYSKLSSWQIAQVARHPQRPYTLDYIAGMFTDFHELHGDRSYSDDPAVVGGM
                     ARFNGAPCMVIGHQKGRDTKEKIYRNFGMPRPEGYRKALRLMKLAEKFGLPLFTFVDT
                     PGAFPGIGAEERGQSEAIGRNLYEMAGLRTPIIVTIIGEGGSGGALAIAIGDYTLMLQ
                     YATYSVISPEGCASILWKSAKHAEEAAETLGITASRLKTLGLIDKVVNEPPGAAHRDH
                     AAMMATLKKALTEALRQAQERPLDALLEAREDKILGYGKFKELNPG"
     gene            1541..2917
                     /gene="tilS"
                     /locus_tag="VHT22_10145"
     CDS             1541..2917
                     /gene="tilS"
                     /locus_tag="VHT22_10145"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX3137961.1"
                     /translation="MITPSSTQEADRHRASAEPRAAPAIVAVDSALTAALSAYTAPGQ
                     RVAVALSGGMDSIVLLDALHALVRRFALSLSALHVDHQLSPHAGRWAEFCAAVCAARS
                     VALTTARVDVARRPGQSLEAAARAARYQKLLAADADIVVLAHHADDQAETVLLQLLRG
                     AGPTGLAAMPHHRARDVAGPALLRPLLELPRATLAAYANARGLAWIDDESNADRRHKR
                     NLLRHDVAPRLAQAFPGYPTVLVRAAGHHAEASALLDELARQDAGNTQDGLDRAALAG
                     LSPGRARNLLRWFLRDEGLRPPSTARLAEMLAQLQTAGTDARTRIVHDGVEIGCHRGK
                     VLVHAAAVGSYARGWRGESSVELPGGVLRFERTHGDGIVAAKFDAAAVTLRSRHGGER
                     IQLAANRPRRAVKKLLYDAQLPIWRRQELPFIWCGDELAAVPGIGVALAFQARPGEAA
                     WRVDWRPK"
     gene            3100..>3666
                     /locus_tag="VHT22_10150"
     CDS             3100..>3666
                     /locus_tag="VHT22_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX3137962.1"
                     /translation="MKSRFFLVVAACALLAFAGSALAQQPIVIKFSHVVAVDTPKGKG
                     AEYFKKLAEERTKGRVKVEVYPNSQLYKDGEEMEALQLGSVQMLAPSVAKFGPLGARE
                     FEVFDLPYIFDNYEDLHKVTEGPIGKALFQKLESKGIVGLAYWDNGFKDMSANKALRK
                     PEDFRGMKMRIQSSKVLDSQMRALGAVPQ"
BASE COUNT          645 a         1241 c         1183 g          597 t
ORIGIN      
        1 cgggctgatc gcggtgacgt tcacgctgaa ccgctggttc gacctcgcgc aacgcctggg
       61 cgcgaaagcg ggcaagccgg ggccgttgat cggcttcctc agcggcgcaa cgtcagggct
      121 gaccagcacg atggcgcacg ccggcggtcc gccgctgctg gtctatctgt tgccccagcg
      181 gatggaaaaa accgtcttcg tcggcaccag cgttgttttt ttcgccatcg tcaactactt
      241 gaagctgatc ccgtacgggc tcctcggcca gttgtcgacg aacaatctcg ggctgtcgct
      301 gctgttcgct ccgctcgccc cgctgggcat ctggctcggg ctccgcctcg aacgccggat
      361 tcccgagaaa cccttctacc gcttgagcta tgcgatcctg tttgcgaccg gctgcaagct
      421 gatctacgat tcgctgacgg cttgatcgag acggcactcc cgccaccccg ccgatagcgg
      481 ggtcggcgtc gattgcgcta tgatcgcgcc ctcgcacagc gaaccattcg ccgccgatga
      541 aacaaacctt tctcgacttc gaacagccga tcgccgagct gcaaagcaag atcgacgagc
      601 tgcgctacgt acacgaagac tcggcggtcg acatctccga cgagatcacc cggctcagca
      661 aaaagagcca gcagctgacc aaggaggtct acagcaagct ttcctcgtgg cagatcgcgc
      721 aggtggcgcg gcacccgcag cggccataca cgctcgacta catcgccggg atgttcaccg
      781 attttcatga gttgcatggc gaccgcagtt attcggacga tccggccgtc gtcggcggga
      841 tggcacgctt caacggcgcg ccgtgcatgg tcatcggaca tcagaagggc cgcgacacca
      901 aggagaagat ctaccgcaac ttcggcatgc cgcgccccga aggctatcgc aaggcactgc
      961 ggctgatgaa gcttgccgag aagttcggtc tgccgctgtt cacgttcgtc gatacgcccg
     1021 gcgcgtttcc cggcatcggc gccgaggagc gcgggcagtc ggaggccatc ggccgcaacc
     1081 tctacgaaat ggccggcctg cgcacgccca tcatcgtcac catcatcggc gaaggcggtt
     1141 ccggcggtgc gctcgcgatc gcgatcggcg actacacgct gatgctgcag tacgcgacct
     1201 actcggtgat ctctcccgaa ggctgcgcgt cgatcctgtg gaagtcggcc aagcacgccg
     1261 aagaagccgc ggaaacgctc ggcatcaccg cgtcacggtt gaagacgctg ggcctgatcg
     1321 acaaggtggt gaacgagccg cccggcgcgg cgcatcgcga tcacgcggcg atgatggcga
     1381 ccctgaaaaa agcgctgacc gaggcgctgc gccaagcgca ggagcgaccg ctcgatgcgc
     1441 tgctcgaggc gcgcgaagac aagatcctcg gctacggcaa gttcaaggaa ctcaacccgg
     1501 gctgacggct cgccgccgcg tccgggctac gagcacggcc gtgattacgc cctcctccac
     1561 ccaggaagcc gaccggcacc gagcttcggc cgagccgcgc gctgcgccgg ctatcgtcgc
     1621 ggtcgattcc gcgctgacag cggcattgtc ggcatacacc gcgccgggtc agcgcgtcgc
     1681 ggtggcgctt tccggcggaa tggactcgat tgtgttgctc gatgcgctgc acgcgctcgt
     1741 ccgccgtttc gcgctcagct tgtcggcgct gcacgtcgac catcagttgt cgccgcacgc
     1801 cggccgctgg gccgagttct gcgccgcagt gtgcgcggcg cgcagcgtgg cgttgacgac
     1861 agctcgagtc gatgtcgcgc gccgtccggg gcaaagtctc gaagccgccg ctcgggcagc
     1921 gcgttaccag aaactcctcg ccgcagacgc cgacatcgtc gtgctagcac atcacgccga
     1981 cgatcaggca gaaaccgtgc tgctgcaatt gctccgcggc gcgggaccga ccgggctggc
     2041 cgcgatgcct catcatcgcg cccgtgacgt cgccgggccg gcgctgctgc gaccgttgct
     2101 cgagctgccg cgcgcgacgc tcgctgccta cgccaatgct cgcggactcg cctggatcga
     2161 cgacgagagc aatgccgaca ggcggcacaa gcgtaacttg ctgcggcacg atgtggcgcc
     2221 gcggttggct caggcgttcc ccggataccc gacagtgctc gtccgcgcgg ccggccacca
     2281 cgccgaagct tcggccctgc tcgacgagct cgcccgccag gacgccggca acacgcagga
     2341 cggactggac cgcgcggcgc ttgccgggtt gtccccgggc cgcgcccgaa atctgctgcg
     2401 ctggttcctg cgcgacgagg ggctgcgacc gccgtccacc gcgcggcttg ccgagatgct
     2461 tgcccaattg cagacggccg gcaccgacgc acgcacgcgc atcgtccacg atggcgtcga
     2521 aatcgggtgc caccgcggaa aagtcttggt tcatgccgcg gctgtcggct cgtacgctcg
     2581 cggctggcgc ggcgaaagct cggtcgaact tcccggcggc gtcttgcgct tcgagcgcac
     2641 tcacggcgac ggcatcgtgg cggcaaaatt cgatgcggct gccgttacgc ttcgatcgcg
     2701 ccacggcggc gaacgcatcc agctcgcggc gaaccggccg cgtcgtgcag tcaagaagct
     2761 tctctacgac gcgcagctgc cgatctggcg gcgccaggag ctgccgttca tctggtgcgg
     2821 cgacgagctg gccgcggttc caggcatcgg cgttgcgctg gcattccagg cccggccggg
     2881 cgaggccgcc tggcgcgtcg actggcgtcc gaagtgaatc aacgcaacac agcgccgcgc
     2941 cggatccggg caagccggcc gagcgggccg atgctccggc gcgcacttgt cgcttccgtc
     3001 cgaggcaggc gcgattgaca agcccgatga ccaagggcta aatgcccgca gaactgacaa
     3061 aaacagtcgt atagcatcca tcttccattg gaggtctcga tgaagtcccg ctttttcctc
     3121 gtagtcgccg cgtgcgcgct gctcgcgttc gccggatcgg cgttggcgca gcagccgatc
     3181 gtcatcaagt tcagccacgt cgtcgcggtc gacacgccca aaggcaaggg cgccgaatac
     3241 ttcaagaaac tcgccgagga acggaccaag ggacgcgtca aggtcgaggt ctatccgaac
     3301 agccagctgt acaaggacgg cgaggagatg gaagctttgc agctcggctc ggtgcagatg
     3361 ctcgctccgt cggtcgccaa gttcggaccg ctgggcgcgc gcgagttcga ggtcttcgac
     3421 cttccgtaca tcttcgacaa ctacgaggac ctgcacaagg tcaccgaagg gccgatcggg
     3481 aaagccctgt tccagaagct cgaaagcaaa ggcatcgtcg gcctggcgta ctgggacaac
     3541 ggcttcaagg acatgagcgc caacaaggcg ctgcgcaagc ccgaggattt ccgcggcatg
     3601 aagatgcgca tccagtcctc gaaggtgctc gacagccaga tgcgcgcgct gggcgccgtg
     3661 ccccag
//
LOCUS       DATANS010000682         1964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_749371, whole genome shotgun sequence.
ACCESSION   DATANS010000682 DATANS010000000
VERSION     DATANS010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749371"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..1818
                     /gene="aspS"
                     /locus_tag="VHT22_10155"
     CDS             40..1818
                     /gene="aspS"
                     /locus_tag="VHT22_10155"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX3137963.1"
                     /translation="MRSEYCGLVDRKHLNQTITVYGWVHRRRDHGGVIFIDLRDREGL
                     LQIVCDPDTPDTLKVAETLRNEFVVRVTGRVRERPAGTVNPNLVSGEVEVLAAEIEVL
                     NPSLTPPFLMDDDTLSENVRLEYRFLDLRRLQMQKNLKLRYKTAMAVRVFLDQHGFID
                     IETPMLTRSTPEGARDYLVPSRVHAGKFFALPQSPQLFKQLLMISGFDRYYQIVKCFR
                     DEDLRADRQPEFTQIDVETSFMTQNEIMVLMEELIRTVFKGVLDIELPKPFPRMSYDD
                     AILLYGSDKPDLRVPLKLAELTDAMKTVAFKVFSGPANSKDGRVAGLLIPKGGELTRG
                     EIDAYTEFVGVYGAKGLAYIKFNEIAKGRDGMQSPIVKNLDDAALKAIVERTGAKDGD
                     LVFFGAGTAKVVADALGALRVKIGHEKGFATAGWQPLWVVDFPMFEWDEEGKRWNAMH
                     HPFTAPADGHEDFLDSDPGRARAKAHDMVLNGWELGGGSVRIHRQDVQSKVFRALNIS
                     AEEAEAKFGFLLQALQFGAPPHGGIAFGLDRLVTMMAGAESIRDVIAFPKTQRAQCLL
                     TQAPNTVDELQLRELHIRLRQEAPKP"
BASE COUNT          373 a          632 c          618 g          341 t
ORIGIN      
        1 cggcccgtcc caccctttct tcttccaccg gtttccatca tgcgcagcga atactgcggt
       61 ctcgtcgacc gcaagcacct caaccagacc atcacggtat acggctgggt ccaccgccgg
      121 cgcgaccacg gcggcgtcat cttcatcgac ctgcgcgacc gcgagggact gctgcagatc
      181 gtgtgcgatc cggacacgcc ggacacgctg aaagtcgccg agacgctgcg caacgaattc
      241 gtcgtgcgcg tgaccggccg tgtgcgcgag cggccggccg gcaccgtcaa tcctaatctc
      301 gtcagcggcg aagtcgaggt gctggccgcc gaaatcgagg tgctcaatcc gtcgctgacg
      361 ccgccgttcc tgatggacga cgatacgctg tcggaaaacg tgcggctcga gtaccgcttc
      421 ctcgacctgc gccggctgca aatgcagaag aatttgaagc tgcgctacaa gaccgcgatg
      481 gccgtgcgcg ttttcctcga ccagcacggc ttcatcgaca tcgagacgcc gatgctgacg
      541 cgctcgacgc ccgagggcgc gcgtgactac ctggtgccga gccgcgtgca cgccggcaaa
      601 ttcttcgcgc tcccgcagtc gccgcagctc ttcaagcagc tcttgatgat ctccggcttt
      661 gaccgctact accagatcgt caagtgtttc cgcgacgagg acctgcgcgc cgaccgccag
      721 ccggaattca cccagatcga cgtcgaaacc tcgttcatga cgcagaacga gatcatggtg
      781 ctgatggaag agctgatccg caccgtgttc aagggcgtgc tcgacatcga gctgccgaag
      841 ccgtttccgc gcatgtcgta cgacgacgcg atcctgctgt acggctcgga caagccggat
      901 ttgcgcgtgc cgctcaaact ggctgagctc accgacgcga tgaaaaccgt cgcgttcaaa
      961 gtgttctcgg gccccgcgaa ttcaaaagac ggccgcgtcg ccggcctgct gattccgaaa
     1021 ggcggcgagc tcacgcgcgg cgaaatcgac gcctacacgg agttcgtcgg cgtttacggc
     1081 gcgaaaggcc tcgcctatat caaattcaac gagatcgcca aaggccgtga cggcatgcag
     1141 tcgccgatcg tcaagaacct cgacgacgct gcattgaagg ccatcgtcga gcgcacgggc
     1201 gccaaggatg gcgatctcgt gttcttcggc gcgggcacgg ccaaagttgt cgccgatgcg
     1261 ctgggcgcgc tgcgcgtcaa gatcggtcac gagaaaggtt ttgcgacggc cggatggcag
     1321 ccgctgtggg tggtggactt tccaatgttc gaatgggacg aagagggcaa gcgctggaac
     1381 gcgatgcacc atccgtttac tgcgccggcc gacggccacg aggatttcct cgacagtgac
     1441 ccaggccgcg cgcgcgccaa ggcgcacgac atggtgctta acggctggga gcttggcggc
     1501 ggctcggtac gtatccaccg ccaggacgtg cagtcgaaag tcttccgcgc gctcaacatc
     1561 agcgcggagg aagcggaggc gaagttcggc ttcctgctgc aggcgctgca attcggcgcg
     1621 ccgccgcacg gcgggatcgc gttcggtctc gaccgcctgg ttacgatgat ggccggtgcc
     1681 gaatcgatcc gcgacgtcat cgcattcccc aaaacccagc gcgcgcagtg tttgcttacg
     1741 caggcgccga atacggtcga cgagctgcag ctgcgcgagc tgcacatacg cttgcggcag
     1801 gaagcgccca aaccctaacg tctcgtggca cggatgccag gggaaagcgc atgcgttcgc
     1861 tgaacaggat tgcagcgttg gcgttgctcg cggcgttgct cgcgccgcct ccggcatcgg
     1921 cgcgaaatga aaacgcgccg gcaaatgaga ttgcggttgc cgca
//
LOCUS       DATANS010000683         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_455071, whole genome shotgun sequence.
ACCESSION   DATANS010000683 DATANS010000000
VERSION     DATANS010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455071"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VHT22_10160"
     CDS             complement(<1..204)
                     /locus_tag="VHT22_10160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3137964.1"
                     /translation="MTLSGGQRQRIAIARAILRDAPLLLLDEATSALDAESETLVQTA
                     LEELMRHRTTLVIAHRLATVLSCD"
     gene            244..987
                     /locus_tag="VHT22_10165"
                     /pseudo
     CDS             244..987
                     /locus_tag="VHT22_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564450.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1149..1349
                     /locus_tag="VHT22_10170"
     CDS             1149..1349
                     /locus_tag="VHT22_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137965.1"
                     /translation="MPYRRGGFALTPPSLVIFLISVVLALIALLVRYAHVSVPIISSA
                     RIFDVLAIAYVVLVIGVLFRRV"
     gene            complement(1353..1607)
                     /locus_tag="VHT22_10175"
     CDS             complement(1353..1607)
                     /locus_tag="VHT22_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137966.1"
                     /translation="MTRAEFAKGSVTFLVVIVAGLLSETYLDRHLAAAETDKKAASND
                     SAKASPPTTPSAACVRKDGSWKNWPWSNVPILSPKCRKDH"
     gene            complement(1677..2126)
                     /locus_tag="VHT22_10180"
     CDS             complement(1677..2126)
                     /locus_tag="VHT22_10180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3137967.1"
                     /translation="MAADFTLRPYRAEDEDAAIELWRTTWQQAYPAIDFTARVAWWRE
                     RWRNELVPNASIIVAEHADTLVGFVTIDGSGYLDQLVVSPDRWGSKLADALVDEAKRL
                     SPGGVTLKVNKDNARAIRFYERNGFGHAGEDVNPTSGRPVLRMTWRP"
BASE COUNT          338 a          684 c          703 g          417 t
ORIGIN      
        1 gtcgcaggac agcacggtgg caaggcggtg cgcgatcacc aacgtggtgc ggtggcgcat
       61 caactcctcc agcgcagtct gcaccaaggt ctcgctttcg gcatcgagcg cggacgtcgc
      121 ctcgtcgagc aacagcagcg gtgcgtcgcg caggatcgcg cgcgcgatcg cgatgcgctg
      181 gcgctggccg ccagacagcg tcacgccgcg ctcgccgagc tgcgcctcga agccgccggg
      241 cagccggcgc gcggcgacgt ggtgttcgac aatgtcagct tcgcctatcc ggcgcggccg
      301 gacgcgctgg ctatcgacgg cgtgtcgctg aaggtcggcg ccggcgagaa ggtggcgatc
      361 gtcggccctt ccggcgccgg aaaaagcacg ctgtttcatc tgttgctgcg cttctacgat
      421 ccgctgcgcg gctcgatctc gttcgacggc gttccgatcc gcgataccga tccgcgcgag
      481 ctgcgcgcgc gcatcgcatt ggtgccgcag gattcggtgg tgttcgcggc gaccgcgcgg
      541 gaaaacatcc gcttcgggcg gcccgatgcc agcgatgcgg aagtcgagcg tgcggccgac
      601 ctggcgcacg caaccgaatt catccgccgc ctgcccggcg gttttgaggc gcaactgggc
      661 gagcgcggcg tgacgctgtc cggcggccag cgccagcgca tcgcgatcgc gcgcgcgatc
      721 ctgcgcgacg cgccgctgct gttgctcgac gaggcgacgt ccgcgctcga tgccgaaggc
      781 gagacgctgg tgcagaccgc gctggaggag ttgatgcgcc accgcaccac gctggtgatc
      841 gcgcaccgcc ttgccaccgt gctgtcctgc gaccggatca tggtgatgga tcagggcagg
      901 atcgtcgaac agggtacgca tgcggagctg gttgcagcca acgggttgta cgcgcgactg
      961 gcgcggttgc aattcgaggg aggctagagc gttttccagc gaagtggata ccggttcgcg
     1021 tcaagaaaac gcgtcaaatc aaaaatccag agccgttcgg acggctctgg aaaaaggcgg
     1081 gaaccagaac gtcgcgtcgc tgttgtagcc ccgttaacta tctcgttctg atcgaacagg
     1141 agaggggcat gccttaccgt cgcggaggtt tcgcgcttac gccgccgtca ctcgtgatat
     1201 ttctgatttc ggtggtgctg gcgcttatcg cccttctcgt tcgttacgcc cacgtttcgg
     1261 tcccgatcat cagttcggcg cggatatttg acgttctggc gattgcctat gtcgtgttgg
     1321 ttattggcgt gctgtttcgg cgggtgtgac gtctagtgat ccttgcggca tttcggcgac
     1381 agtatcggca cgttcgacca cggccagttt ttccaggagc cgtctttcct gacgcacgcc
     1441 gccgacggtg tggttggcgg tgatgctttg gcgctatcgt ttgaagctgc ttttttgtcc
     1501 gtctcggcgg cggcgaggtg ccgatccaga taggtctcgg acagcaatcc ggcgacgatc
     1561 accaccaaaa acgtcaccga gcccttggca aattcggccc gcgtcatcgt ttcgtctccg
     1621 ttgttgcgtt gcgcagtcgg cttaccgcac cccacctctc cgacctgtga tgacgatcac
     1681 ggtctccagg tcattcgcaa gaccggacgc cccgacgtcg gattgacgtc ctccccggca
     1741 tgcccaaagc cattgcgctc gtagaagcgg atggcgcggg cgttgtcctt gttgaccttg
     1801 agtgtaacgc cgccgggcga caggcgtttc gcctcatcga ccagcgcgtc ggcaagtttc
     1861 gagccccagc gatcggggct gaccacgagc tggtcgagat agccgctgcc atcgatggtc
     1921 acgaacccga ccagcgtgtc cgcgtgttcg gcgacgatga tcgaagcatt cggcaccagc
     1981 tcgttgcgcc agcgctcccg ccaccacgcc acgcgcgccg tgaaatcgat cgcgggatag
     2041 gcttgctgcc aggtcgtccg ccacagctcg atcgccgcgt cttcgtcctc ggcgcgatag
     2101 gggcggagcg tgaaatccgc ggccactacc gctccgtcag ct
//
LOCUS       DATANS010000684         1820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_203054, whole genome shotgun sequence.
ACCESSION   DATANS010000684 DATANS010000000
VERSION     DATANS010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203054"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VHT22_10185"
     CDS             complement(<1..965)
                     /locus_tag="VHT22_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3137968.1"
                     /translation="MSQGSQFRLLRQRRFLPFFGAQAFGAFNDNIYKNVLVIVATYHA
                     ASYTQLDPELLTNMAGGLFILPYVLFSGIAGQLADRFDKALVLKVVKAAEIVIMTIAA
                     LGFAAHSIEVLLSALFLMGVHSTFFAPAKYGLLPEVLQETELVGGNALVEMGTFLAIL
                     LGTLAAGLLAAEGSNGAIATALIGVAAIGFTVSLAIPALRPAAPDLRIDWRPWTSTWD
                     NIRAARESRAVFQSILGISWFWFYGALVLAQLPLFAKNVLGGSEQIVTLLLVLFSAGI
                     GVGSLLCERLSGRKVEIGLVPFGSIGLTAFAVDLYFAVPDAPPGIG"
     gene            1066..1635
                     /locus_tag="VHT22_10190"
     CDS             1066..1635
                     /locus_tag="VHT22_10190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX3137969.1"
                     /translation="MQAVSDEQLIQWVADGDASCLGTLFERHHRSVYQYCLQLTRSPA
                     ASEDLVQDVFLKLLQKAGSYRGEGSCKAWIFNIARNVTFDYLRRAERRTVSDDDADEN
                     AEPLVDHRSTEQVAAGQQNLRLVARALAEIPAAAREVIWLGRFVFEDYEDLARALGCT
                     ASAARVRMHRAMQQLNTTFTRMNGVPIDV"
     gene            1628..>1820
                     /locus_tag="VHT22_10195"
     CDS             1628..>1820
                     /locus_tag="VHT22_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137970.1"
                     /translation="MSEKRKGGVPFESDDAAEQQLWSDLEKLPEEAPSGRLRRRFYDE
                     LENADRRMHRRRRLSGWLTA"
BASE COUNT          353 a          591 c          595 g          281 t
ORIGIN      
        1 ccgatgcccg gcggcgcgtc cggcactgcg aaatacagat cgacggcgaa agcggtcaag
       61 ccgatcgaac cgaacggcac caggccgatc tcgaccttgc gacccgacaa gcgttcgcac
      121 aacagcgagc cgacgccgat gccggccgag aacagcacga gcagcagcgt cacgatctgc
      181 tcgctgccgc cgagaacgtt cttcgcgaac agcggtagct gcgcgagcac gagcgcgccg
      241 tagaaccaga accacgagat tccgaggatc gactggaaca ccgcacgcga ctcgcgcgcg
      301 gcgcggatgt tgtcccaggt cgaggtccac ggccgccagt cgatgcgcag atccggcgcc
      361 gcgggacgca gtgcggggat cgccaggctg accgtgaagc cgatcgcagc gacgccgatc
      421 aacgcagtcg cgatcgcgcc gttgctacct tccgctgcca gcaatccagc cgcgagcgtg
      481 ccgagcagga tggcgaggaa cgttcccatc tcgaccagcg cgttgccgcc gacgagctct
      541 gtctcttgca gcacttccgg cagcaggccg tatttcgcgg gcgcgaagaa cgtcgagtga
      601 acgcccatca ggaacagcgc gctgagcagc acttcgatgc tgtgtgcggc gaacccgagc
      661 gccgcgatcg tcatgatcac gatctccgcg gctttgacga ccttgagtac cagcgccttg
      721 tcgaagcggt cggcgagctg accggcgata cccgagaaca gcacatacgg caggatgaag
      781 agcccgcccg ccatgttggt cagcagctcg ggatcgagtt gcgtgtagct cgccgcgtga
      841 taggtagcga cgatgacgag cacgttcttg tagatgttgt cgttgaaggc gccgaacgcc
      901 tgcgcgccga agaacggcag aaaccgccgc tggcgcagca gccggaactg cgaaccctga
      961 ctcacccctg gggcactccg ctcgggcctt cgtattgttg cggcgtatag cgtaacgttt
     1021 tccggcctcg gccgtctttg tcggcgaggc gagcgaacaa cgatcatgca ggcagtgtcc
     1081 gacgaacaac tgatccagtg ggtggctgac ggcgatgcgt cgtgcctcgg cacgctgttc
     1141 gagcgccacc atcgcagcgt ctaccagtac tgcctgcaac ttacgcgtag ccccgcggcg
     1201 agcgaggacc tcgtccagga cgtgttcctc aagctgttgc agaaggctgg aagctaccgc
     1261 ggcgaaggca gctgcaaagc gtggatcttc aacatcgccc gcaacgtgac gttcgactat
     1321 ctgcgtcgcg ccgagcgccg caccgtcagc gacgacgacg ccgacgagaa tgccgagccg
     1381 ctggtcgatc atcggtcgac cgagcaggtc gcagccggcc agcagaacct gcgtctggtc
     1441 gcgcgggcgt tggcggagat tccggcggca gcgcgcgaag tcatctggct cgggcgtttc
     1501 gtattcgaag actacgagga cctggcccgc gcgctcggct gcaccgccag cgctgcgcgt
     1561 gtgcgcatgc accgcgcgat gcaacagctc aacaccacgt tcactcgcat gaacggagta
     1621 ccgatcgatg tctgagaaac gcaagggcgg cgtgccgttc gagagcgacg acgccgcgga
     1681 acaacagctc tggagcgatc tcgagaagct gccggaagag gcgccttcgg gtcgtctgcg
     1741 ccgcaggttc tacgatgagc tcgagaacgc ggaccgacgt atgcatcgtc gccggcgctt
     1801 gtctggctgg ctcacggcgc
//
LOCUS       DATANS010000685         4091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_413246, whole genome shotgun sequence.
ACCESSION   DATANS010000685 DATANS010000000
VERSION     DATANS010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413246"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VHT22_10200"
     CDS             complement(<1..245)
                     /locus_tag="VHT22_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX3137971.1"
                     /translation="MPNAISPVMHYVAAREPGPPEVLALAETATPAPKPGEVLIAVAY
                     AGVNRPDCIQRAGHYPPPPDASPIIGLEVAGAVVALG"
     gene            352..723
                     /locus_tag="VHT22_10205"
     CDS             352..723
                     /locus_tag="VHT22_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137972.1"
                     /translation="MKKLIALLVFVAVVIGAAWIAFGADYSDGYRVGQVIKLSRSGYV
                     FKTWEGTLDFGFLQQDPTLGTATRLWDFSVHDGDDAVRKDIDEAIAVNAKVKLFYHEK
                     YFRWSFFGDTKHFVYKLERVH"
     gene            1154..1600
                     /locus_tag="VHT22_10210"
     CDS             1154..1600
                     /locus_tag="VHT22_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX3137973.1"
                     /translation="MPERKTLERAAEDKREGKAPSTQAGEFVREEMEHVREGKHGARS
                     AKQAIAIGLSKARRAGVALKPPAKGKAPAKTRRSAKLATAAGKRKAAVSPRRSRATTD
                     ALKREGSSAASPTALSRHAKAVARKRGPAARSAAARKAARTRSRKS"
     gene            complement(1699..2904)
                     /locus_tag="VHT22_10215"
     CDS             complement(1699..2904)
                     /locus_tag="VHT22_10215"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3137974.1"
                     /translation="MLDDILGRYRSFLRLPDVARLLATALITRLPIASLTLAMLMHLR
                     ALTGSFALAGLTVGVDLAASAIAAPVLGRIIDRRGPHAVMLVTGIVCPVALVFILLAD
                     RLALSTAALVAAAAIAGAFAPPVTVLTRTVWRYRFDDPAERKTAFALDAVLIELAFTL
                     GPVLVAVLLAVASPRVAFTAALLLVALAVPVFALSPALKYWRHQPGVERRLLGPLADP
                     RLLAVYAVTFLLMFALGLLEIGYAGFATAAAMPAFAGILIAINSIGSGTGGLLYGGAQ
                     LAMPVERQLPRLLLLVVLPLAAHTVTGSPWLLAALALVAGVGIAPALTVVMLLISANA
                     PSRYATEAFTWSTTFLVGGLGAGHALGGRIVEGYGARAVFGVAAGAAAAAAISALAVR
                     QRKLAPVDR"
     gene            complement(2949..>4091)
                     /locus_tag="VHT22_10220"
     CDS             complement(2949..>4091)
                     /locus_tag="VHT22_10220"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX3137975.1"
                     /translation="AYFKAHHPAAFMAANLSAMMDDTDKLRQFYDDATGLGLSILPPD
                     VNASNYRFEPVDGARIRYGLGGIKGTGRAAIEAIVAARAKAPFTDLFDFCRRVDKRSV
                     NRRAIEALIRAGAFDAVDTRRASLLVSVGAALDHGERAERAASQVSLFGEEGGAGPAA
                     PALIAAREWSEAERLAQEKTSLGFYLSGHPYHAHAAELSRLVQQSLSALKPTQTPVRI
                     AGIVVQLRTQTSRRGKMAFVTLDDGRDQPEIVIFNETFDANRHLLREDAVLVAEVKVQ
                     QRVGDDGQVQGLRIIADAVYDLAGARRKWARALRLSCNGSSSAQRLFDLIEPFRRGHC
                     PVTINYSNQASGGDIELGSDWQVNLDEHLLAGLREWLAPENVTVVY"
BASE COUNT          728 a         1366 c         1417 g          580 t
ORIGIN      
        1 tcgcccagcg cgacgacggc gccggcgacc tcgagtccga tgatcggcga ggcatcgggc
       61 ggcggcggat agtgtccggc gcgctggatg cagtccgggc gattgacgcc ggcgtaagca
      121 accgctatca gcacctctcc gggcttcggg gcgggcgtcg ccgtctcggc gagcgccagg
      181 acctcgggtg gcccgggctc gcgggcggcg acgtagtgca tgacggggga aatggcgtta
      241 ggcatcgctg gcgcggtcgc cggtcgggta ggcaagcatt gtagctcggg ctccgcgtac
      301 cctctgccgt gcatccgggc tacacttgga cggcgtcccg tccatccgcc gatgaaaaag
      361 ctcatcgcac tcctcgtctt tgtcgccgtc gtcatcggcg cggcgtggat cgcctttggc
      421 gctgactaca gcgacggcta ccgcgttgga caggtgatca agctgtcccg ctcgggctac
      481 gtgttcaaga cctgggaggg gacgctggac ttcggcttcc tgcagcagga tccgacgctg
      541 ggcacagcca cgcggctgtg ggacttcagc gtccacgacg gcgacgacgc ggttcgcaag
      601 gatatcgacg aagcgatcgc ggtcaacgcg aaggtcaagc ttttctacca cgagaagtat
      661 ttccgctggt cctttttcgg cgacaccaag catttcgtct acaagctcga gcgcgtccac
      721 tgagggccga ggacagtcgc gcccattcgt gatgcgcttg ccccggtgcg gcgcgcaccg
      781 cgggcgacca gtacgccgat tcccggaacg ttttttgctg ccgctggttc aggcgagctt
      841 ggttgcgcgc tttttacagg agacgagcga tggcgcaaat gcaaggttca atgggatcga
      901 acagctacag caagtgcgct cacccggggt gcatgtgcga tgtcgcgccc ggcaagaagt
      961 attgctccga ctattgccag caaaacgccg ggagcaaatc ggcctcgtcc gatggctgcg
     1021 gttgcggtca ctccgcctgc aagcgcggct gaacggccgc ggagttcagg gcggtggcgc
     1081 ctcgtgcgtc cggcaactat tgctgccgct tgtaagcttt actcgatcac gattcgaagc
     1141 gaaggagaaa accatgcctg aacggaaaac actggagcgc gcggccgaag acaagcgcga
     1201 aggaaaggcg ccgagcacgc aggccggcga gttcgtccgc gaggagatgg agcacgtgcg
     1261 cgaaggcaag cacggggccc gatccgcgaa gcaggcgatc gccatcggct tgtcgaaggc
     1321 acgccgtgcg ggcgttgcgc tcaagccacc cgccaaaggc aaggcgccgg caaagactcg
     1381 ccgcagcgcc aagctcgcca ccgccgccgg caagcgcaag gcggccgttt cgccgcggcg
     1441 atcgagggcg acgaccgacg cgctgaagcg cgaaggcagc agtgccgcca gtccgaccgc
     1501 cctgtcgcgg catgcgaagg cggtggccag gaaacgcggc ccggcggcgc gttcggccgc
     1561 agcccggaag gcggcgcgca ctcgctctcg aaagtcttaa ccgcaacggc ggacctaacc
     1621 gcaacggcgg acttaaccgc aacggcggac gcgaccgtac gggatcgtgc cctctcggtg
     1681 ccgatccaaa acgtccggct accgatcgac gggcgccagc ttgcgctggc ggacggctag
     1741 cgcgctgatc gccgcggctg cggcggcgcc ggccgcgacc ccgaacaccg cgcgtgcgcc
     1801 gtaaccttcg acgatcctgc cgccgagcgc gtgaccggcg ccgaggccgc cgacgaggaa
     1861 tgtcgtcgac caggtaaacg cctccgtcgc gtagcgtgac ggggcgttgg cgctgatcag
     1921 gagcatcacc accgtcagcg ccggagcgat gccgacaccg gcgaccagcg ccagcgcggc
     1981 cagcagccac ggcgagcccg tcaccgtatg cgccgccagc ggcagcacga ccagcagcag
     2041 taatcggggc aactgtcgct cgaccggcat cgcgagctgc gcgccgccat aaagcaggcc
     2101 gccggtgccg ctgccgatcg aattgatcgc gatcaggatg cccgcgaacg ccggcattgc
     2161 agcggcagtc gcgaagccgg cgtagccgat ctcgagcagc cccagcgcaa acatcagcag
     2221 gaacgtcacg gcataaacgg cgagcaggcg cggatcggcc agaggcccga gcaggcgacg
     2281 ctcgacgccg ggctggtgac gccagtattt gagcgccggc gagagggcga agaccggcac
     2341 tgcgagcgcg accagcagca aggcggcggt gaacgcaacg cgcggcgatg cgacggccag
     2401 cagcacggcg acgagcaccg ggcccagcgt gaaagcgagc tcgatcaaaa ccgcatcgag
     2461 agcgaaagcg gtcttgcgct cggcgggatc gtcgaagcgg tagcgccaca cggtgcgcgt
     2521 caagacggtg accggcggtg cgaaggcgcc cgcgatcgcg gcggcggcca cgagagctgc
     2581 ggtcgacagg gccagcctgt ccgccagcag tatgaagacc agcgccaccg ggcagacgat
     2641 gccggtaacg agcatcacgg cgtgcggtcc gcggcggtcg atgatgcgtc ccagcacggg
     2701 ggcggcgatc gccgacgccg cgagatcgac gccaacggtc agaccagcga gcgcgaacga
     2761 tccggtcagc gcacgcaggt gcatcagcat cgccagcgtc agactcgcta tcggcagccg
     2821 tgtgatcagc gcggtcgcga gcagccgtgc gacgtccggc aggcgcagaa acgaccggta
     2881 gcgaccgagg atgtcgtcga gcatcgagtg gcatcgccgc gcacgccgcc gctcgcgcgc
     2941 gaagtcgcct agtagacgac ggtaacgttc tccggcgcca gccattcgcg caggccggcg
     3001 agcaggtgct cgtccaggtt cacctgccaa tcgctgccca gctcgatgtc cccgccgctt
     3061 gcctggttgg agtaattgat cgtcaccggg cagtgtccgc ggcgaaacgg ctcgatcaga
     3121 tcgaacaggc gctgcgccga gctcgagccg ttgcacgaaa gacgcagcgc gcgcgcccat
     3181 ttccgccgcg cgcccgcgag gtcgtagacg gcatcggcga tgatccgcag accctgaacc
     3241 tggccatcgt cgccgacgcg ctgctgcacc ttcacttcgg cgaccagcac ggcgtcctca
     3301 cgcagcagat ggcgatttgc atcgaaggtt tcgttgaaga tgacgatctc cggctggtcg
     3361 cggccgtcgt cgagagtcac gaacgccatc ttgccgcggc gcgacgtttg cgtgcgcagc
     3421 tgcacgacga tgccggcgat gcggaccggc gtctgcgtgg gcttcagcgc gctcaagctc
     3481 tgctgcacca gccgggagag ctcggccgcg tgcgcgtgat acgggtggcc cgacaggtag
     3541 aagccgaggc tcgtcttttc ctgcgccagc ctctcggcct ccgaccactc gcgcgcggcg
     3601 atcaaggccg gtgctgccgg tcccgcgccg ccttcttcgc cgaacagcga cacttgcgag
     3661 gccgcacgct cggcgcgctc gccatgatcg agcgcagcgc cgaccgacac gagcaggctc
     3721 gcgcgtcttg tgtcgacggc atcgaaagcg ccggcgcgga tcagcgcctc gatggcgcgg
     3781 cgattgacgc tgcgcttgtc gacgcggcgg cagaaatcga acaggtcggt gaacggcgct
     3841 ttcgcgcgcg ccgcgacgat cgcctcgatc gcggcccggc cggtaccctt gatgccgccc
     3901 aggccgtagc ggatgcgcgc gccatcgacc ggctcgaagc ggtaattcga cgcgttgacg
     3961 tccggcggca atatcgacag gccgagccct gtcgcgtcgt cgtagaactg ccgcagcttg
     4021 tcggtatcgt ccatcatcgc cgacaggttg gcggccatga acgcggccgg atgatgcgcc
     4081 ttgaaatacg c
//
LOCUS       DATANS010000686         2863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_579871, whole genome shotgun sequence.
ACCESSION   DATANS010000686 DATANS010000000
VERSION     DATANS010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579871"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VHT22_10225"
     CDS             complement(<1..575)
                     /locus_tag="VHT22_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX3137976.1"
                     /translation="MGALNTLSAVEAARQIAAGKIKSEALVRDCLERIAARDGEVQAW
                     EHIDEAAALAQARAADSNPGRGLLQGVPVGVKDLIDTLDMPTGYGSPVYAGHRPAWDA
                     PCVALTRAAGGIVLGKTVTTEFANMHPNKTRNPHNPAHTPGGSSSGSAAAVADFMVPL
                     AFGTQTAGSIIRPAAYCGVVGYKPTYNSLVLA"
     gene            complement(575..1693)
                     /locus_tag="VHT22_10230"
     CDS             complement(575..1693)
                     /locus_tag="VHT22_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004136962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX3137977.1"
                     /translation="MPDAVRLTRELLKFNTINPPGAEQACAQHLGEILLRAGFTVDYA
                     EFAPTRTSLVAKLGGRPDKRPLGFTGHIDTVPLGAAAWKADAFGASMEGGKLYGRGSS
                     DMKSGVAAFVVAALKMAQQLKAGPGVTLVITADEECGCGGAAHLVKQGGLLGEVGALV
                     IGEPTANYPLVGHKGALWLDVMTRGITAHGSMPERGDNAIYKAAGAIQKLREFDFETP
                     AHDLMGSPTLNVGTVHGGLNINSVPDSTVIGVDIRTIPGQDHALLRSRMQRLLGETVE
                     ITPTIDVGSIYSDPAQPWIQHVFDVTQAHLKIRPQAKTATYFTDAAVLKDAYRDVPMV
                     VLGPGEPQLAHQTDEYCLVDCIEQAVDIYVELIKDWSS"
     gene            complement(1714..2700)
                     /locus_tag="VHT22_10235"
     CDS             complement(1714..2700)
                     /locus_tag="VHT22_10235"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX3137978.1"
                     /translation="MTAGDVLLEVRNLVKHFPVRKGVFGRVTGQVHAVDDVSFSIASG
                     ETLGLVGESGCGKSTTGKAVLKLVEPTSGSIDWRGRRIDELSASNMRPYRRELQAVFQ
                     DPYSSLNPRMRAEDIVGEPIRNFAAAGRAEIRERVASLFDKVGLRQDQMIKYPYEFSG
                     GQRQRLGIARALALRPKLIVCDEPVSALDVSVQAQVINLLMDLQNEMQLSYLFVAHDL
                     AVVEHISHRVAVMYLGKIVELTDKRSLFAHPQHPYTEALLSAVPTPDPRARKKRIILG
                     GDVPSPINPPPGCRFHTRCPYAFERCRVEEPLMQEVRAGHFVACHLRDKPNA"
     gene            complement(2697..>2863)
                     /locus_tag="VHT22_10240"
     CDS             complement(2697..>2863)
                     /locus_tag="VHT22_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX3137979.1"
                     /translation="MVPSLKEEIGGCLFAPRCDYATERCRREYPPLEEKAPGHFAACW
                     ESDRLRGVGT"
BASE COUNT          498 a          963 c          882 g          520 t
ORIGIN      
        1 gccagcacca ggctgttgta agtgggcttg tagccgacca cgccgcaata ggcggccggc
       61 cggatgatgg agcccgcggt ttgcgtgccg aaggccagcg gcaccatgaa atcggcgacc
      121 gcggccgccg agccggacga cgaaccgccg ggcgtatgcg cgggattgtg cgggttgcgc
      181 gtcttgttcg gatgcatgtt ggcaaattcg gtggtcaccg tcttgcccag tacgatgccg
      241 ccggcggcgc gcgtcaaggc cacgcacggc gcatcccacg ccggccgatg ccccgcgtat
      301 accggcgagc cgtaaccggt cggcatgtcg agggtgtcga tcaggtcctt tacgcctacg
      361 ggcacaccct ggagcagacc acggcccggg ttcgagtcag ccgcgcgggc ctgcgcgagc
      421 gcggcagctt catcgatatg ctcccacgcc tggacttcgc cgtcgcgcgc ggcgatgcgc
      481 tcgaggcaat cacgcaccag cgcttcggat tttattttgc cggcggcgat ctggcgcgcg
      541 gcctcgaccg ccgacagcgt attcaatgca cccattacga actccagtcc ttaatcagct
      601 cgacgtaaat atcaacggcc tgctcgatgc agtcaaccag gcaatattcg tcggtctggt
      661 gcgcgagctg cggttcgccg gggcccagta cgaccatcgg gacgtcgcga taggcgtcct
      721 tcaacaccgc cgcatcggtg aaataagttg ccgttttagc ctgtggccgg atcttaaggt
      781 gcgcctgggt cacgtcgaac acgtgctgga tccacggctg ggcgggatcc gaataaatcg
      841 acccgacgtc tatcgtgggc gtgatttcga ccgtctctcc gagcaggcgc tgcatgcgcg
      901 agcgcagcag cgcatggtcc tggcccggta tcgtgcggat gtcgacgcca atcacggtcg
      961 agtcgggcac ggaattgatg ttgagcccgc cgtgcacagt gccgacattg agcgtcggac
     1021 tacccatcaa atcgtgtgcc ggcgtttcga aatcgaactc gcgcagtttc tggatcgcgc
     1081 cagcggcttt atatatggca ttgtcgccgc gctccggcat cgacccgtgc gcggtgatgc
     1141 cgcgcgtcat cacgtcgagc cacaacgcgc ccttgtgccc gaccagcggg taatttgcgg
     1201 tcggctcgcc gatcaccagc gcgccgactt cgcccaataa cccgccctgc ttgacgagat
     1261 gcgccgcgcc gccgcagccg cactcctcgt ccgccgtgat cacgagcgtc acgcccgggc
     1321 ccgccttgag ctgctgcgcc atcttgagcg ccgccaccac gaacgcggcg acgccgcttt
     1381 tcatgtcgct cgagccgcgc ccatacagct tgccgccttc catgctcgcg ccaaatgcgt
     1441 ccgctttcca cgcggccgcg cccagcggga ccgtatcgat gtgcccggta aatccaagcg
     1501 gccgcttgtc cggccggccg ccgagtttcg ccacgagact cgtgcgagtc ggtgcgaatt
     1561 ctgcatagtc gacggtgaaa cccgcgcgca gaaggatttc gccaagatgc tgcgcgcatg
     1621 cctgctcggc gcccggtgga ttgatcgtgt tgaacttcag caattcacgc gtgagccgca
     1681 ctgcgtccgg catcgctgcc atggaacttc tccttaagcg ttcggcttat cgcgcaagtg
     1741 gcacgcgaca aaatgtccgg ctcgcacttc ctgcatcaac ggctcctcga cgcggcagcg
     1801 ctcgaatgcg tacgggcagc gcgtatgaaa gcggcagcca ggcggcggat tgatcgggct
     1861 gggcacgtcg ccaccgagaa tgatgcgctt ttttcgtgcg cgcgggtcag gtgtgggcac
     1921 cgctgacagc aatgcctcgg tatacgggtg ctgcggatgc gcgaacagcg agcgcttgtc
     1981 ggtgagctcg acaatcttgc cgaggtacat gaccgccacg cgatggctga tgtgctcgac
     2041 caccgcgaga tcgtgcgcga cgaacaagta cgaaagctgc atctcgtttt gcaggtccat
     2101 cagcaaattg atcacctgcg cctgtaccga cacgtccagc gccgacaccg gctcgtcgca
     2161 cacgatgagc ttaggccgca gcgcgagcgc gcgcgcaatg ccaagacgct ggcgctggcc
     2221 gcccgagaac tcgtatgggt acttgatcat ttgatcctgg cgcaggccga ccttgtcgaa
     2281 gagcgacgca acgcgttcgc gaatttcagc gcgcccggcc gctgcgaaat tgcgaattgg
     2341 ttcgccgacg atatcttcag cacgcatgcg cggattgagc gacgaatacg ggtcctggaa
     2401 caccgcctgc agctcgcgcc ggtacggtcg catattgctc gccgacagct cgtcgatgcg
     2461 ccgcccccgc cagtcgatcg agccgctggt gggctcgacg agcttcaata ctgccttacc
     2521 ggtcgtcgat ttgccgcaac cgctttcgcc gaccagcccc agcgtttccc cgctcgcaat
     2581 tgaaaagctc acgtcgtcga ccgcgtgcac ctggccggtc acgcgcccga acacgccctt
     2641 gcgcaccgga aagtgcttca cgagattcct gacctccagc agcacgtcgc ccgcggtcat
     2701 gtgccgactc cgcgcaaacg atcggactcc cagcacgccg cgaaatggcc cggggccttt
     2761 tcctcgagcg gcggatattc gcggcggcag cgctcggtcg cgtagtcgca gcgcggcgca
     2821 aacaggcaac ccccgatttc ctccttcaac gacggcacca tgc
//
LOCUS       DATANS010000687         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_160453, whole genome shotgun sequence.
ACCESSION   DATANS010000687 DATANS010000000
VERSION     DATANS010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160453"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VHT22_10245"
     CDS             <1..320
                     /locus_tag="VHT22_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3137980.1"
                     /translation="AMVQDLVDHVLGAGVWLDQQPANREKAVQIAATPAYFNQDPKIL
                     RFVMQNPTDRVTYGDLRMIRSEFEELMQLSMQAGTIKHPIPYEKYVDERFALTAKPAV
                     IPL"
     gene            332..898
                     /locus_tag="VHT22_10250"
     CDS             332..898
                     /locus_tag="VHT22_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX3137981.1"
                     /translation="MRSFLLTLAALVAGPAASAPALKAGVFEPPRAAPQFSLQSSSGG
                     KLELHSYRGKVVILGFGFTWCTDVCPVTLATLAQARRKLGAAADEVQVVYITVDPARD
                     NPARMKEYLHAFDPTFIGGTGGADELAAVRKDYGIVAEKHVKGSSYSVAHSSFIYLID
                     RRGQLRALMPYGRGPDDYVHDLQILLAE"
     gene            958..1326
                     /locus_tag="VHT22_10255"
     CDS             958..1326
                     /locus_tag="VHT22_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3137982.1"
                     /translation="MLAWAGFTPIETRTGADREELFEIPRGTWARRMKGEDLGILPDE
                     IRLTLGIRDMLVLRNLDEVPQIFGPTLMMPGQSFRLPFEQAAEYQFACSAHASGQMRV
                     IVYPYPATPWARIMWRARRL"
     gene            1323..>1608
                     /locus_tag="VHT22_10260"
     CDS             1323..>1608
                     /locus_tag="VHT22_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3137983.1"
                     /translation="MNRIKTSLPALGVIAALLLAWWGAIVATQSLIFPTPWGVVTGAI
                     ELLANGTLWRHIAASLLRVGTGFALAVCIAVPLGLWMGWVRGAYLPLNPIF"
BASE COUNT          271 a          532 c          515 g          290 t
ORIGIN      
        1 cagccatggt gcaggacctg gtcgaccatg tgctcggcgc cggcgtatgg ctcgatcagc
       61 agccggcgaa ccgcgagaag gccgtgcaga tcgccgccac tccggcgtac ttcaaccagg
      121 atccgaagat cctccgcttc gtgatgcaga acccgaccga ccgcgtgacc tacggcgacc
      181 tgcgcatgat ccgcagcgag ttcgaagagc tgatgcagct gtcgatgcag gctggcacca
      241 tcaaacatcc catcccgtac gagaagtacg tcgacgagcg ctttgcgctc accgcgaagc
      301 ccgcggtcat cccgttatga acgcgcgcac cgtgcgttcc ttcttgttga cgctcgctgc
      361 actcgtagcg ggaccggcgg ccagcgcgcc ggcgctgaaa gccggagtct tcgaaccgcc
      421 gcgcgcggct ccgcagttct cgctgcaaag ttctagcggc ggcaagctcg agctgcacag
      481 ctatcgcggc aaagtcgtga tcctcggctt cggcttcacg tggtgcacgg acgtgtgccc
      541 ggtcacgctc gcgactctgg cgcaggcgcg gcgcaagctc ggcgctgcag ccgacgaggt
      601 ccaggtcgtt tacataaccg tcgatcccgc gcgcgacaat ccggcacgga tgaaggagta
      661 cttgcacgcc ttcgatccga cgttcatcgg cggcacaggt ggagccgatg agctcgccgc
      721 agtacgcaag gactacggga tcgtcgcgga gaaacatgtc aaaggcagca gctattctgt
      781 tgcgcactcc tccttcattt acctgatcga ccgccgcggg cagctgcgtg cactgatgcc
      841 ctacgggcgt ggcccggacg attacgtgca cgatctgcag attctcctcg cggagtagcg
      901 acgatgcgtt cgccgctgcg gaccgtcctg ctggcatgcg gtgcagtcgg cgtcggcgtg
      961 ctcgcctggg ccggcttcac gccgatcgaa acccggaccg gcgccgaccg cgaggagctg
     1021 ttcgagattc cgcgcggaac ctgggcgcga cgcatgaagg gcgaggatct cggcatcctg
     1081 ccggacgaga tccgcttgac gctcggaatc cgcgatatgc tggtgctgcg gaacctggac
     1141 gaggtgcccc agatcttcgg cccgacgctc atgatgccgg gccagagttt ccggctcccg
     1201 ttcgaacagg cggcggaata tcagttcgcc tgctccgcgc acgcgagcgg gcagatgcgg
     1261 gttatcgtgt atccgtatcc agcgacgccg tgggcccgga tcatgtggcg cgccagaaga
     1321 ctatgaacag aatcaaaacc agtctgcccg ctctcggcgt catcgcggcg ctgctcctcg
     1381 cctggtgggg cgcaatcgtt gccacgcaga gcctgatatt ccctaccccg tggggagtcg
     1441 tcaccggcgc aatcgagcta ctggcgaacg gcacgttgtg gcggcacatc gctgcatcgt
     1501 tgctgcgcgt cggcactggt ttcgcgctcg ccgtctgcat cgccgtcccg ctcggactgt
     1561 ggatgggctg ggtgcgcggc gcgtacctcc cgctcaatcc gattttcc
//
LOCUS       DATANS010000688         4130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_789625, whole genome shotgun sequence.
ACCESSION   DATANS010000688 DATANS010000000
VERSION     DATANS010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789625"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..692
                     /locus_tag="VHT22_10265"
     CDS             186..692
                     /locus_tag="VHT22_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX3137984.1"
                     /translation="MTIKVGDRLPAGMLSEYVEVATEACPIGPNTFQIDALTKGKKVV
                     LFGLPGAFTPTCSGKHVPGYVEHFGELKAKGVDEIICMSVNDAFVMGAWAKDQKTGGK
                     VRMMGDGSAAYTKALGLELDLTARGMGVRCQRFSALVDDGVVKSLNVEAPGKFEVSDA
                     QTMLNQLG"
     gene            complement(777..1631)
                     /locus_tag="VHT22_10270"
     CDS             complement(777..1631)
                     /locus_tag="VHT22_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX3137985.1"
                     /translation="MAKLKGLGRGLDALLSGSTETPSRESLQTIALDRIRPGKYQPRS
                     RMDEASLDELAASIKAQGVMQPILVRPVDANRFEIIAGERRWLAARRAGLAEVPALVR
                     EVPDQAALALALIENIQREDLNPLEEAQGIKRLIEEFGLTHEAAGEAVGRSRSAVTNL
                     LRLTQLAKPVQDYLLSGELDMGHARALLGLPAAQQAAAAAKVVALGMSVRDTERMMQA
                     AQHPAPKKKRRLRGKSADADLARLENELSDALGTSVHIEATRDGAGRIVVAYSSLDQL
                     DGILAKLR"
     gene            complement(1624..2454)
                     /locus_tag="VHT22_10275"
     CDS             complement(1624..2454)
                     /locus_tag="VHT22_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3137986.1"
                     /translation="MTKIIAVANQKGGVGKTTTSVNLAASLVAMKRRVLLVDLDPQGN
                     ATMGAGVDKRALTHTVYQVLLGEKKIADVRVASPSGGFDLVPANRELAGAEVELVDLP
                     EREMRLKDALAEALKQMRTAISEVVGEYDFILLDCPPSLSLLTLNGLCAADSVLIPMQ
                     CEYYALEGLSDLVQTIRKVRAHLNPRLEIEGLLRTMFDPRNTLALNVSADLQKHFGDK
                     LYRTVIPRNIRLAEAPGFGVPALTLDRASKGALAYLALAGEMLRRDEAASPQPGVVNG
                     "
     gene            complement(2451..3092)
                     /gene="rsmG"
                     /locus_tag="VHT22_10280"
     CDS             complement(2451..3092)
                     /gene="rsmG"
                     /locus_tag="VHT22_10280"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX3137987.1"
                     /translation="MTPAARLADGVATLGLAALVGADIQARLLAYLALLDKWNRTHNL
                     TAIREPERMITHHLLDSLAVLPHLPSRAGLRLIDIGSGGGLPGIPLAIVRPDWQLTLL
                     DSNFKKATFLRQAAIELPLPNVEVVAARVEEYAPAVRFDIAISRAFSDLAMFALASGN
                     LLAPEGRLVAMKGVYPHEEIAHLPAGIGVTSTPALKIPGVEGDRHLVIMERAA"
     gene            complement(3089..>4130)
                     /locus_tag="VHT22_10285"
     CDS             complement(3089..>4130)
                     /locus_tag="VHT22_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3137988.1"
                     /translation="AERKSHQIFLEPEGLTTNEIYPNGISTSLPFDVQLDLVHSIKGC
                     EHAHILRPGYSIEYDYFDPRALKATLETKAIAGLFFAGQINGTTGYEEAAAQGLLAGI
                     NAALLAGGHDGWCPRRDQAYLGVLVDDLITRGVSEPYRMFTSRAEYRLQLREDNADLR
                     LTEAGRRIGVVDDVRWDAFCRKRDAVAAEMERLKSTWVNPRVVDQASAMRVLGQGLER
                     DYALIDLLGRPGVGYASLMSLPGAGDPVADPEVAAQVEVATKYAGYIGRQNEEIARHA
                     GQEALRLPADVDYRKVRGLSVEVQQRLNQHRPETIGQAARLPGMTPAAISLLLVHLKR
                     GFDAPVARKLSA"
BASE COUNT          699 a         1360 c         1337 g          734 t
ORIGIN      
        1 tctccttcag cgggtagtcg cgatcggact gccgaagtta cgctttcgcg cgcgcgcaga
       61 caagtactcg gcgtggttcg acgccgcgac gatttccggt acaatttacg atctgtcgct
      121 tatcgcgtgc tcaagttgca cgcgcgcgat agccgtcgcc acacatcacg cggaaggaaa
      181 atgtcatgac gatcaaagtc ggcgaccggc tgcctgccgg catgctgagc gagtacgtcg
      241 aggtcgcgac cgaagcatgt ccgattggac ccaacacgtt ccagatcgat gcgctgacca
      301 agggcaagaa ggtggtgttg ttcggcttgc ccggcgcatt caccccgacc tgctcgggca
      361 agcacgtgcc gggttatgtc gagcacttcg gcgagctaaa agccaagggc gtcgacgaga
      421 tcatctgcat gtcggtcaac gacgccttcg tgatgggcgc gtgggcgaag gatcagaaga
      481 ccgggggcaa ggtccggatg atgggcgacg gcagcgccgc gtacacgaag gcgctcggcc
      541 tcgagctcga cctgaccgca cgcggcatgg gcgtgcgttg ccagcgtttt tcggcgctgg
      601 tcgacgacgg cgtcgtcaaa tcgctgaacg tggaggcgcc gggcaagttc gaagtctccg
      661 acgcgcagac gatgctgaat caactcggct gacccgcgac ctcgcttgca aacggcgcct
      721 gcgggcgccg ttattgtttg cgctgcgcta tcgagcgcgt agctggcggc gcgagatcag
      781 cggagctttg ccagtattcc gtcgagctgg tcgaggctcg aataagcgac gacgatacgg
      841 ccggcgccgt ctcgcgtcgc ttcgatgtgc acgctggtgc caagagcgtc ggacagctcg
      901 ttttccagcc gcgccagatc agcgtcggcg cttttcccgc gcagccgccg ttttttcttc
      961 ggcgccggat gctgcgcggc ctgcatcatg cgctcggtgt cgcggaccga catgccgagg
     1021 gcgacgactt tggcggcagc ggcggcctgc tgcgccgccg gcaatcccag caatgcgcgg
     1081 gcgtgcccca tgtcgagctc gcctgacagc aagtagtcct gcaccggctt ggcaagctgc
     1141 gtcagccgca aaagattcgt caccgcgctg cgcgagcggc cgacggcctc tccggccgcc
     1201 tcgtgcgtca gtccaaattc ttcgatcaac cgcttgatgc cctgcgcctc ctcgagagga
     1261 ttcagatcct cgcgctggat gttctcgatc agcgccagcg ccagcgccgc ctgatcgggc
     1321 acttcgcgca ccaacgcggg cacctcggct aacccagccc gccgcgccgc cagccagcgt
     1381 cgttcgccgg cgatgatctc gaagcggttc gcgtcgaccg ggcgcaccag tatcggctgc
     1441 atgactccct gcgccttgat cgacgcggcc agctcgtcga gcgaggcttc gtccattcgc
     1501 gaacgcggtt ggtatttgcc cgggcgaatg cgatcgaggg cgatggtctg cagcgattcg
     1561 cggctcggcg tctcggtcga gcccgacaac agcgcatcga gccctcgccc gagccctttc
     1621 aacttagcca ttgacgactc ccggttgcgg cgacgcggct tcgtcgcggc ggagcatttc
     1681 accggcgagg gcgagatacg ccagcgcgcc cttcgatgcc cggtccagag tgagcgcggg
     1741 caccccgaag cccggcgctt cggcgagccg gatattgcgc gggatcaccg tgcggtagag
     1801 cttgtcgccg aaatgcttct gcaggtcggc cgacacgttg agcgccagcg tattgcgcgg
     1861 gtcgaacatc gtgcgcagca gcccctcgat ctcgaggcgc gggttcagat gggcgcgaac
     1921 ctttcgtatc gtctgcacca ggtcggacaa gccttcgagc gcgtagtact cgcactgcat
     1981 cgggatcagc acgctatccg ccgcgcacag cccgttcaag gtcaatagcg ataatgaagg
     2041 cgggcagtcg agcaggataa agtcgtattc gccaacgacc tccgaaatcg ccgtgcgcat
     2101 ttgcttcagc gcctcggcga gcgcgtcctt gagccgcatt tcgcgctcgg gaagatcgac
     2161 tagctcgact tcggccccgg cgagctcgcg gttggccggc accagatcga acccgccgct
     2221 gggcgaagcg acgcggacgt cggcgatctt cttttcacca agcaggactt gatatacggt
     2281 gtgagtaagt gcacgcttat cgacgccggc tcccatggtc gcgttgcctt ggggatcgag
     2341 gtcgaccaga aggacgcggc gcttcatcgc gaccagactg gcggcgagat tgacgctggt
     2401 cgtggttttg ccgacgccgc ctttctgatt ggccacggcg atgatcttgg tcacgcggcg
     2461 cgctccatga tcaccagatg acgatcgcct tcgacgccgg gaattttcag cgccggggta
     2521 ctcgtcacac cgatcccggc gggcaaatgc gcgatctcct cgtgcggata gacccccttc
     2581 atcgcgacca ggcgtccttc cggagcgagc aggttgccgc tggcgagggc aaacatcgcg
     2641 agatcggaga aggcgcgtga aatcgcaatg tcgaacctga cggccggcgc gtattcctcg
     2701 acccgcgccg cgacgacctc gacattcggc agcggcaatt cgatcgcggc ctggcgaagg
     2761 aacgtcgcct tcttgaagtt gctgtcgagc agggtgagtt gccaatccgg gcgcacgatc
     2821 gccaacggga tgcccggcag gccgccgccg ctgccgatgt cgatcagcct taggccggcg
     2881 cgcgaaggca gatgcggcag caccgccagc gaatccagca gatgatgcgt gatcatccgt
     2941 tccggctcgc ggatcgccgt caggttatgg gttcgattcc atttgtcgag cagcgccaga
     3001 taggcgagta gcctggcctg gatgtcggcg ccgacaagcg cggccagtcc cagcgtcgcg
     3061 acgccgtcgg ccagccgcgc ggccggcgtc atgccgaaag cttgcgcgcg accggcgcat
     3121 cgaagccgcg cttcaagtgg acgagcagca gcgaaatcgc cgccggggtc atcccgggaa
     3181 ggcgagcggc ctgtcctatc gtctccggcc gatgctggtt gagcctttgc tgcacctcga
     3241 ccgaaagccc ccgcaccttg cggtagtcga cgtctgcggg gaggcgcaag gcctcctgcc
     3301 cggcgtgacg cgcgatttcc tcgttctgcc gcccgatata gccggcgtac ttagtcgcga
     3361 cttcgacttg cgcggccacc tccgggtcgg ccacagggtc acccgcaccg ggcaggctca
     3421 tcaggctcgc gtacccgacg ccgggccggc ccaacagatc gatcagcgca tagtcgcgct
     3481 cgagcccctg ccccagcacg cgcatcgcgc tggcttgatc gacgacccgc ggattgaccc
     3541 aggtcgactt cagccgctcc atctccgcgg cgacggcgtc gcgcttgcga caaaacgcat
     3601 cccaccgaac gtcgtcgacg acaccgatcc gtcgtccggc ctcggtcagg cgcagatcgg
     3661 cgttgtcctc gcgcaattgc aggcggtatt cggcgcgact ggtgaacatc cggtagggct
     3721 cgctgacccc gcgcgtaatc aggtcgtcga ccagtacccc cagatatgcc tggtcgcggc
     3781 gcgggcacca gccgtcgtga ccgccggcca gaagagccgc gttgatgccc gccagcaggc
     3841 cctgcgctgc ggcttcctcg tatccggtgg tgccgttgat ctggccggcg aagaacaagc
     3901 ccgcgatcgc tttagtctcg agcgtggcct tcagcgcgcg cggatcgaaa taatcgtatt
     3961 cgatcgaata gccggggcgc aggatgtgcg cgtgctcgca ccccttgatc gaatgcacga
     4021 gatcgagctg cacgtcgaac ggcagcgacg tcgaaatgcc gttcgggtag atctcgttcg
     4081 tcgtgagccc ttccggctcg agaaagatct gatggctctt ccgctccgcg
//
LOCUS       DATANS010000689         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_708049, whole genome shotgun sequence.
ACCESSION   DATANS010000689 DATANS010000000
VERSION     DATANS010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708049"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VHT22_10290"
     CDS             complement(<1..268)
                     /locus_tag="VHT22_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="HEX3137989.1"
                     /translation="MIAHLPNLLAPKFFILYGFIASGMYIHYRGRIRHRFYRQLTDHS
                     TFMAPYNVLMYFFSAVPNRPYVDVKAFPELKKLSDSWQMLREEAM"
     gene            complement(294..1568)
                     /locus_tag="VHT22_10295"
     CDS             complement(294..1568)
                     /locus_tag="VHT22_10295"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEX3137990.1"
                     /translation="MAGSADPFLAQRGQPTRCGIDSVEIARIERLLRETPAEDLIRYF
                     SPQELTDSGDGPGRAASLAARFAAKEACAKLFPRELALAEIEPADFSIERDAYGAPQV
                     VCSARAGVVLGRQRLRAIAVSLTHDRISASAVALALPAQADAPLAGRLIYRLFPLRRR
                     VILENLHRVFGAAVPEAEIIGLAQAHYAHLWRLAGEFVRFRWLSEQKKSALIRVDNVE
                     ALARALERGKGALILTGHFGNFEVATIAGLRHFPHMRGRFHFIRRAIRPRWLDALVNR
                     RFRQGGFGVVGKRGSLDVILERLEAGDLVVFPFDQHASPPDGIAVDFFGHPAGTFKSL
                     AIMALATGAPVVPASSWREPDGSHVLRFEEALPLIECVKVNEEIRRNTRAYNAMLEIL
                     VLRHPEQWFWVHRRWKLGPAAGRREGQAAQAL"
     gene            complement(1573..>2115)
                     /locus_tag="VHT22_10300"
     CDS             complement(1573..>2115)
                     /locus_tag="VHT22_10300"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HEX3137991.1"
                     /translation="EGAGALALETEDAAQKRNAAALGEVLGGGYAAEGEGLLAIREDG
                     DGLSRAITQALAEAGLAPADIGMIVAHGNGTPLSDASEAAALRGVFGTGDTAPPVTAF
                     KWATGHLIAAAGIMETVIALAALRERIVPGIATLTSLDPDCDGLRVSSAPQVPRGDTA
                     LILCRGFAGTNAALIVRALR"
BASE COUNT          366 a          728 c          698 g          323 t
ORIGIN      
        1 gcatcgcttc ctcgcgcagc atctgccaac tgtcgctcag cttcttgagc tccgggaacg
       61 ccttcacgtc gacgtaggga cggttgggca cggcggagaa aaaatacatg agcacgttgt
      121 acggcgccat gaacgtcgaa tgatcggtca gctggcgata gaaccgatgc cggatgcggc
      181 cgcgataatg gatgtacatc cccgaagcga tgaagccgta gaggatgaaa aatttcggcg
      241 ccagcaggtt gggcagatgc gcgatcatcg tcggtcttcg gtggtggcgg ccattatagc
      301 gcctgagcgg cctggccctc tcgacgcccg gccgccggcc cgagcttcca gcgccggtgc
      361 acccagaacc actgctcggg gtggcgcagg accagtattt ctagcatcgc gttgtacgca
      421 cgggtgttgc gccggatttc ctcattgacc ttgacgcact cgatcagcgg cagcgcttcc
      481 tcgaagcgca gcacatgcga gccgtcgggc tcacgccagc tcgatgccgg cacaaccggc
      541 gcgccggtcg cgagtgccat gatcgcgagg cttttgaacg ttcccgcagg atggccaaaa
      601 aagtcgactg cgatgccgtc gggtggcgag gcgtgctgat cgaacgggaa cacgaccagg
      661 tcgccagcct cgagtcgctc gaggatcaca tcgagcgacc cgcgcttgcc caccacgcca
      721 aagccacctt gccggaagcg ccggttcacc agcgcgtcga gccagcgcgg ccgaatcgcg
      781 cggcggatga aatgaaagcg cccccgcatg tgcggaaaat ggcgcaggcc cgcgatcgtc
      841 gccacctcga aattgccgaa gtgtccggta aggatcagcg cgcccttgcc acgctcgagt
      901 gcacgcgcca gcgcttcgac gttgtcgacg cggatcagcg ccgacttctt ttgctccgac
      961 agccagcgaa agcggacaaa ctcgccggcg agccgccaca gatgcgcgta atgcgcctgc
     1021 gccagcccga tgatctcggc ttcggggacc gccgcgccga acacacggtg gagattttcc
     1081 aggatgacac ggcggcgcag cgggaacagg cggtagatca gccggcccgc cagcggtgcg
     1141 tcggcttgcg caggaagcgc cagcgcgacc gcggaggcgc ttatccggtc gtgcgtcagc
     1201 gatactgcga tcgcccgcag tcgctgccgt ccgagcacga cccctgccct ggcgctgcag
     1261 acgacctgcg gcgcgccgta ggcatcgcgc tcgattgaaa aatcggcggg ttcgatctcg
     1321 gctagtgcga gctcacgggg aaaaagcttg gcgcaggcct ccttggcggc aaagcgcgcc
     1381 gccaggcttg ccgcccggcc cggcccgtca ccgctgtcgg tgagctcctg cggcgaaaaa
     1441 tagcggatca ggtcttccgc cggcgtctcg cgcaacaggc gctcgatgcg cgcgatttcg
     1501 acgctgtcga tgccgcagcg cgtcggctgc ccgcgctgcg ccaagaacgg gtcggcggag
     1561 cccgccatcc gatcagcgca gcgcgcggac gatcagtgcg gcattggtcc cggcgaagcc
     1621 gcggcacaga atcagcgccg tgtcgccacg cggaacctga ggcgcggagg acactcgcag
     1681 gccgtcgcaa tcaggatcga gcgaggtcag cgtggcgatg cccgggacga tgcgctcgcg
     1741 cagcgccgcc agcgcgatca ctgtctccat aatgccggcc gcggcgatca gatgcccggt
     1801 cgcccacttg aatgccgtga ccggcggcgc cgtgtctccg gtgccgaaca caccgcgcag
     1861 cgccgccgcc tcggatgcgt ccgacagcgg cgtgccgttg ccgtgagcga cgatcatccc
     1921 gatatccgcc ggcgcgagcc cggcttcggc cagcgcctgc gtgatcgcgc ggctaaggcc
     1981 atcgccgtcc tcgcgaatcg cgagcagtcc ctcgccttct gctgcatacc cgccgccgag
     2041 cacctcaccc agcgcagcgg cgttgcgctt ttgcgcggcg tcctcggtct cgagcgcgag
     2101 cgcacccgcg ccctc
//
LOCUS       DATANS010000690         2501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_370815, whole genome shotgun sequence.
ACCESSION   DATANS010000690 DATANS010000000
VERSION     DATANS010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370815"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /locus_tag="VHT22_10305"
     CDS             <1..1492
                     /locus_tag="VHT22_10305"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX3137992.1"
                     /translation="DRDYYLLDDDAKLSAARAKYRTHVDQMMTMAGDSDATRDASAIV
                     ALETELAKVQWTKVELRDPIKAYNKVGLDKLGALAPGYDWKRYLDSAGVEGKVTYLIV
                     GQPSYITAFAKILDQTPLPVWKAYFRWHLLSAFAPYLSKPYVDANFAFFGTALNGTPE
                     IRPRWKRGVQLVDDSIGEDLGRVYVEQYFPPESKARMDALVANLLAAYRQSIGDLDWM
                     SSTTKAKALDKLAKFMPKIGYPKKWRDYSTLRIVPTDLVGNVMRANEFEYRRNIAKLG
                     KPIDRGEWFLTPQTVNAYYNPELNEIVFPAAILQPPLFDAQADDAVNYGAIGAVIGHE
                     ISHGFDDQGSQYDGDGNLHDWWTAEDHDKFTAKTKALVQQYSAYEPVAGYHVNGELTL
                     GENIADNSGLAIAYKAYRISLDGKEAPVIDGLTGDQRFFMGSSQGWRTKMRDDYTVRL
                     IKSDPHSPNTLRSNAAVINQDAFYRAFDVKPGDKMYLPPEKRVTIW"
     gene            complement(1512..>2501)
                     /locus_tag="VHT22_10310"
     CDS             complement(1512..>2501)
                     /locus_tag="VHT22_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3137993.1"
                     /translation="VAYDTLIFCVGSTTNDFGIPGVATHAISLDTPLDAERFHRRLLA
                     AAVRADGEAGEGRSAHVDIVIIGAGATGVELAAEIRQTTRTHALYGLDKLDPARDIHL
                     TLLEAAPRILPQLGERIAKAATELLGTLGIAVRTGERVVTVDEKGVHTANGATLHADL
                     IVWAAGIQAPPLLTQLDGLEVNRANQLVVTATLQTTRDPDIFAFGDCAAAPWLGRGKE
                     GALVPPRAQAARQQAALLVHAMRARLAGRPLPEFHFRDFGSLVSLGELSAVGNLMGRL
                     IGGSMLIQGLIARAMYTSLYKLHQASIHGYFRVTLDTIGRFLRRRMEPRVKLH"
BASE COUNT          495 a          815 c          786 g          405 t
ORIGIN      
        1 ggaccgcgac tactatctgc tggatgacga cgccaagcta agcgcggccc gcgccaagta
       61 ccgcacgcac gtcgaccaga tgatgacaat ggccggcgat tccgatgcga cgcgggacgc
      121 ctcggcgatt gtcgcgctcg aaactgaact ggccaaagtg caatggacga aggtcgagct
      181 gcgggaccca atcaaggcct acaacaaggt ggggctcgac aagctcggtg cgctcgcacc
      241 cggctacgac tggaagcgct acctagacag cgccggcgtc gagggcaagg tgacctatct
      301 gatcgtcggc cagccgagct acatcaccgc tttcgcgaaa atcctcgacc agacgccgtt
      361 gccggtctgg aaagcctatt tccgctggca cctgctaagc gctttcgcgc catatctctc
      421 caagccttat gtcgacgcca atttcgcgtt tttcggcacg gcactcaacg ggactccgga
      481 gattcgtcca cgctggaagc gcggcgtgca gctcgtcgac gattcgatcg gggaggattt
      541 gggcagggtc tacgtcgagc aatacttccc gccggagagc aaggcgcgga tggatgcgct
      601 ggtcgcgaat cttctggccg cctaccggca aagcatcggt gatctcgact ggatgagctc
      661 gacgaccaag gcgaaggcgc tcgacaagct cgcgaagttc atgcctaaga tcggctaccc
      721 gaaaaaatgg cgcgactatt cgacgctaag gatcgtgccg accgacctgg ttggcaacgt
      781 gatgcgcgct aacgaattcg aatatcggcg caacatcgcc aagctcggca agccgatcga
      841 tcgcggcgaa tggttcctga caccgcagac ggtcaatgcc tattacaacc ccgagctcaa
      901 cgagatcgtg tttcccgcgg ccatcctgca gccgccgctg ttcgacgcgc aagccgacga
      961 cgcggtcaat tacggcgcga tcggcgcggt gatcggccac gagatcagcc atggcttcga
     1021 cgaccagggc agccaatacg acggcgacgg caacctgcac gactggtgga cggccgagga
     1081 tcacgacaaa tttaccgcca agaccaaggc cctggtccag cagtacagcg cctatgagcc
     1141 ggtggccggc tatcacgtca acggcgagct aaccttgggc gagaacatcg ccgacaactc
     1201 cggtctcgcg atcgcataca aggcgtaccg catctccctc gacggaaagg aagcgccagt
     1261 aatcgacggg ctgaccggcg atcagcgctt tttcatgggg tcgtcgcagg gctggcgcac
     1321 caagatgcgc gacgactata cggtgcgcct gatcaagagc gacccgcact cgcccaatac
     1381 gctacgatcc aacgccgcgg tcatcaatca ggatgccttt taccgcgcgt tcgacgtcaa
     1441 gcccggcgac aagatgtacc tgccgccgga aaagcgcgtg acgatctggt agcgtgccgc
     1501 gccgccgcgc gtcaatgcag cttgacgcgc ggctccatgc ggcgtcgcag aaagcgcccg
     1561 atcgtgtcga gcgtcacgcg gaagtagccg tggatcgacg cctgatgcag cttatacagc
     1621 gacgtgtaca tcgcgcgcgc gatcagtccc tggatcagca tgctgccgcc gatgagacgg
     1681 cccatcaggt tgccgaccgc cgacaattcg ccgagcgaga ccagggaacc gaagtcgcga
     1741 aagtgaaatt cgggcagcgg cctccccgcc aggcgagccc gcatcgcatg caccagtagc
     1801 gccgcctgtt gccgcgccgc ctgcgcccgc ggcggcacca gcgcgccttc cttgccgcgt
     1861 cccagccaag gcgccgcggc gcaatcgcca aaggcgaaga tgtcgggatc gcgcgtcgtc
     1921 tgcaaggtcg ccgtgacgac cagctggttg gcgcggttga cttccagacc atccagctgc
     1981 gtcagcagcg gcggcgcctg aatgcccgcg gcccacacga tcagatcggc gtggagcgtc
     2041 gcgccgttgg cggtgtgcac gcctttctcg tcgacggtga cgacgcgctc gccggtccgc
     2101 acggcgatgc ccagcgtgcc gagaagctcg gtcgccgcct tggcgatacg ttcgcccagt
     2161 tgcggcagaa tgcgcggcgc cgcctcaagc agcgtcagat gaatgtcgcg cgcgggatcg
     2221 agcttgtcca gcccgtagag ggcgtgggtg cgtgtcgtct ggcgtatctc cgcggccagc
     2281 tcgacaccgg ttgcgcctgc gccgatgatc acgatgtcga catgggccga tcggccttct
     2341 cccgcttcgc cgtcagcacg cactgcggcg gcgagcagtc ggcggtgaaa gcgctccgca
     2401 tccagcggcg tgtccagcga aatcgcgtgc gtggcgacgc ccggaatgcc gaaatcgttg
     2461 gtcgtgctgc caacgcagaa aatcagcgtg tcgtaggcaa c
//
LOCUS       DATANS010000691         3033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_829274, whole genome shotgun sequence.
ACCESSION   DATANS010000691 DATANS010000000
VERSION     DATANS010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829274"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..1150
                     /gene="dapE"
                     /locus_tag="VHT22_10315"
     CDS             5..1150
                     /gene="dapE"
                     /locus_tag="VHT22_10315"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEX3137994.1"
                     /translation="MTTSSSNAPTLDLARALIARRSQTPDDAGCQDLLGKRLANSGFR
                     CESLVANGVSNLWARRGDVHPIVCFAGHTDVVPSGPLDAWESDPFDPSQRDGYLYGRG
                     AADMKSSLAAFVVAIEQFVAARPDHPGSIALLITSDEEGPAIDGTVRVVERLRSRGDT
                     LDYCVVGEPSSVERLGDMIKNGRRGTLSGKLIVRGVQGHVAYPQLARNPIHLAAPVIA
                     ELAATRWDEGDAYFPPTSWQVSNIHAGTGATNVIPGTLELDFNFRYAPVHTREDLERA
                     FLAILARHGLDHEIRWTGSGKPYLTARGQLVDVAGAAIREVCGIVPELSCTGGTSDGR
                     FIADICAEVVELGPVNATIHKLNERVAVADLEPLAAIYRGILERLLI"
     gene            1176..2075
                     /gene="prmB"
                     /locus_tag="VHT22_10320"
     CDS             1176..2075
                     /gene="prmB"
                     /locus_tag="VHT22_10320"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX3137995.1"
                     /translation="MSTVMQELETVRDWIRYAVSRFNTAGVFFGHGLADAYEEAAYLI
                     LHKLSLPSDRIDMFLDSRVTQAERAELLQIIERRVDERIPAAYLTHEAWLGGFRFYVD
                     ERVIIPRSFIAELLPDGLAPWIPDSAAVQSTLDLCTGSGCLAVLLAHHYPNADVDATD
                     ISPDALTVAQRNVADHGLQDRINLIRSDLLSNLTEKTYDLIICNPPYVTAMAMEELPA
                     EYRHEPDIALAGGDDGLDAVRIVLAKAPQFLAPEGVLVVEIGHNRAAAEKAFPRLPFT
                     WLATGDSDDSVLMLKRDELVAGA"
     gene            complement(2090..>3033)
                     /locus_tag="VHT22_10325"
     CDS             complement(2090..>3033)
                     /locus_tag="VHT22_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX3137996.1"
                     /translation="SGETLIIPNSALIKNRVHVLARRGDLRIPWRRPVEINVGYEWPP
                     SRVINVIEAALARADMPNVARAPGPLCTCAGFEASSIKYVVLYWLTDLSRDLLTDSVV
                     RLHAFAALSREGMDVPITRAEIFWNPASDVRAEEAVRERDTRIALLVSLELFRPLTDL
                     ERKALSAELKPAPFVTGDVITREGEPAESLYILARGQVGIFRDAAQPAESPRQRLATL
                     SAPGYFGEMGLLTGQARTATVVAEGEVLCYRLDKPGFEAILKARPEMAEGLSQTLAAR
                     QAANDATLASLSAEARARATGSRASDLVQKIRAFFGM"
BASE COUNT          508 a         1026 c          984 g          515 t
ORIGIN      
        1 aagcgtgacg accagttcgt ccaacgcgcc gacactcgat ctggcgcgcg cgctgatcgc
       61 gcgtcgctcg cagacacccg acgacgccgg ctgccaggac ctgctcggca aacgcctggc
      121 caatagcggc ttccgctgcg aaagtctggt cgccaacggg gtcagcaatc tgtgggcgcg
      181 tcgcggcgac gtgcatccga tcgtctgttt cgccgggcac accgacgtcg tgccgtcagg
      241 cccactcgac gcctgggagt ccgacccgtt cgaccccagc cagcgcgacg gctatctgta
      301 cggccgcggc gcagcggata tgaagtcctc gctcgccgcg ttcgtggtcg cgatcgagca
      361 attcgtcgcc gcgcggcccg accacccggg ctcgatcgcg ctgctgatca cctccgatga
      421 ggaaggaccg gcgatcgacg ggacggtgcg tgtagtcgag cgcctgcgca gccgcggcga
      481 cacgctcgat tactgtgtcg tcggcgagcc ttcgtcggtc gagcgcctcg gcgacatgat
      541 caagaacggc cggcgcggaa cgctgtcggg caagctgatc gtgcgcggcg tgcagggcca
      601 cgtcgcctat ccgcagctgg cgcggaatcc gatccatctc gccgcaccgg tgatcgccga
      661 gctggctgcc acacgctggg acgaaggcga cgcctacttt ccaccgacca gctggcaggt
      721 gtcgaacatc cacgccggga ccggggccac caatgtcatt cccggcacgc tcgagcttga
      781 tttcaatttc cgctatgcgc cggttcacac gcgcgaggat ctcgaacggg cgttcctcgc
      841 gatcctggcg aggcacggcc tggatcatga gattcgctgg accggctcgg gcaagcccta
      901 tctgaccgca cgcgggcagc tggtggacgt cgccggcgcg gcgatacgcg aggtctgcgg
      961 catcgtcccc gaactctcct gcaccggagg cacatcggat ggtcgtttca tcgccgacat
     1021 ttgtgccgag gtcgtcgagc tcgggccggt caacgctacg atccacaagc tcaacgagcg
     1081 cgtcgctgtc gctgatctgg agcctttggc ggcgatctac cgtggcatac tcgagcggtt
     1141 actgatctga acgcgccgcg gcgcgccgat tatcgatgag cacagtcatg caggagctgg
     1201 agaccgttcg cgactggata cgctacgcgg tgtcgcgctt caacaccgcc ggtgttttct
     1261 tcggtcacgg gctggcagac gcctacgagg aagcggcgta cctgatattg cacaagctgt
     1321 cgctgccgtc ggaccggatc gacatgttcc tcgacagcag ggtgacgcaa gccgagcgtg
     1381 cggagctgct gcagatcatc gagcgccggg tcgacgagcg catccccgcc gcctacctca
     1441 cgcacgaggc gtggctcggc ggcttccgct tctatgtcga cgagcgcgtc atcattccac
     1501 gctcgttcat cgccgaactg ttgcccgacg ggctcgctcc ctggattccc gactccgctg
     1561 ccgtgcagag cacgctcgat ctgtgcacag ggtcgggctg cctggcggtg ctgcttgcac
     1621 atcactatcc gaacgccgac gtcgacgcca ccgatatttc tcccgacgcg ctgaccgtcg
     1681 cgcagcgcaa cgtcgccgat cacgggctgc aggatcgcat caaccttatc cgctccgacc
     1741 tgttgtcgaa cctgaccgaa aagacctacg acctgatcat ctgcaatcct ccatacgtca
     1801 cggcgatggc gatggaggaa ttgccggccg aataccggca tgagcccgac atcgccttgg
     1861 ccggcggcga cgacggcctc gatgcggtgc ggatcgttct agccaaggcg ccgcaatttc
     1921 tcgcgcctga aggcgtgctg gtggtcgaaa tcggccacaa ccgggcggcc gccgaaaagg
     1981 cgtttccgcg cttgcccttc acgtggcttg cgaccggcga cagcgacgac agcgtgctga
     2041 tgctgaagcg cgatgagctc gttgccggcg cgtaggtctg cgcgccgggc tacatcccga
     2101 agaacgcccg gattttctgc accaggtcgg aagcgcgcga gcccgttgcg cgggcgcgcg
     2161 cctcggcgga aagcgaagcg agcgtcgcat cattggccgc ctgtcgggca gccagcgtct
     2221 gcgacagccc ttccgccatt tcgggacgcg cctttaggat cgcttcgaat cccggcttgt
     2281 cgagccggta gcagagcacc tcgccttcgg caaccacggt cgccgtgcgc gcctggccgg
     2341 tcaacagccc catctcgccg aaatagccgg gcgccgacag ggtggcaagc cgctgccggg
     2401 gcgactcggc cggctgcgcc gcatcgcgaa agatgccgac ctggccgcgg gctagaatgt
     2461 acagcgattc ggccggctcg ccttcgcgcg tgatgacgtc gcctgtgacg aatggcgccg
     2521 gcttgagctc cgccgacagc gccttgcgtt cgaggtcggt caacggccgg aacaattcca
     2581 gcgagaccaa tagcgcgatt cgcgtatcgc gctcgcgcac cgcctcttcg gcgcggacgt
     2641 cgctggccgg attccagaag atctcggcac gggtaatcgg aacatccata ccttcgcgcg
     2701 acaaagccgc gaatgcgtgc agccggacga ccgaatcggt cagcaggtcc cggctgaggt
     2761 cggtcagcca gtacaggacg acgtacttga tcgagctcgc ctcgaaaccg gcgcaggtgc
     2821 acaaagggcc tggcgcgcgc gccacgttgg gcatgtcggc gcgggcgagc gccgcctcga
     2881 taacgttgat cacccggctg ggcggccatt cgtagccgac gttgatctcg accgggcgcc
     2941 gccacggaat gcgcagatcg ccgcgccgcg ccagcacgtg gacgcgattc ttgatcaggg
     3001 cggaattcgg gatgatcagc gtttcgccgc tgt
//
LOCUS       DATANS010000692         2074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_538398, whole genome shotgun sequence.
ACCESSION   DATANS010000692 DATANS010000000
VERSION     DATANS010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538398"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VHT22_10330"
     CDS             <1..369
                     /locus_tag="VHT22_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX3137997.1"
                     /translation="AAFSLSWRWLADYSYVANWLLFATLCFFHLREIGPSRLKAKAAT
                     LAALTAVAIATQSVAQAEMRSRFGQQSYVRHLKPPALRLAAPQSEEAFFADAARIKPR
                     LDNSRKDAPPGGGIFSAFDE"
     gene            complement(392..1612)
                     /locus_tag="VHT22_10335"
     CDS             complement(392..1612)
                     /locus_tag="VHT22_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX3137998.1"
                     /translation="MSPRADTAATIAAAIAAMGPGPLTEEGIARHVAPLFSRVLHPDR
                     PVYLANHSLGRPLDATADDVAEAVGLWQSQLGNAWDAWLAEITAYRARLAALMHAPRA
                     DCVVPKTSAGQGLRAILNSYDRPPRVVATRGEFDSIDLILRQYALRKRIELRFVEARA
                     DGMFEADELLAAIRDVDLVVVSQVLFNTGQVLPALPALIGAAHSSGARVLLDVYHSLG
                     VLPVDIGALDADFAIGGSYKYLRGGPGAGYMYLHPRHLDAGFSTLDIGWFAKKNKFDY
                     VRPDPPEFAAGGDALLESTPPVLTCYQARAGQLFTVAVGVERLREYSLAQQRTLIGHL
                     SERGVRGEGGSDDRGAFVVVRSGQARAWAGELERRGVLVDSRGDLLRLCPDLLSTKAE
                     LASAAQRLGDIAAR"
     gene            complement(1707..>2074)
                     /locus_tag="VHT22_10340"
     CDS             complement(1707..>2074)
                     /locus_tag="VHT22_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019453009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEX3137999.1"
                     /translation="APSLYDEFLRHLSRRGLPVPPACVERDWSFPHQRNAALVEVFKT
                     IYENPQQYWDAYEMAEKLVDVDEAFALWRFRHMKTVERIIGYKTGTGGSSGVAFLRRV
                     LEHTFFPELIDVRTVIGAP"
BASE COUNT          344 a          709 c          693 g          328 t
ORIGIN      
        1 gccgcattct cgctgtcgtg gcgctggctt gccgattaca gctatgtcgc caactggctg
       61 ctgttcgcga cgctgtgctt cttccatcta cgcgagatcg ggccgagccg gctcaaggcc
      121 aaggcggcga cgctggccgc gctcaccgcg gtggccattg cgacgcaatc ggttgcgcag
      181 gcggaaatgc gttcgcgctt tggccaacag tcgtacgtgc ggcatctgaa gccgccggcg
      241 ctgcgactcg cggcgccgca aagcgaagag gccttctttg ccgatgcggc gcggatcaag
      301 cccaggctcg ataactcccg caaggacgcc ccgcccggcg gcggtatttt ctccgcgttc
      361 gacgaatagc tttggttggc tgcggcaggc gctaccgcgc tgcgatgtca cccagacgct
      421 gggcggcgga ggcgagttcc gctttcgtcg acagcaggtc ggggcaaagg cgaaggaggt
      481 cgccgcgcga gtcgacgagc acgccgcggc gctcgagctc accggcccac gcgcgggctt
      541 gtccgctgcg cacgacgaca aacgcgccgc gatcgtcgga gccgccttcc ccgcgcacgc
      601 cgcgctcgct taagtgcccg atcagagtgc gctgctgcgc cagcgaatat tcgcgcagcc
      661 gctcgacgcc gaccgctacc gtgaacagct gcccggcgcg cgcctgatag caggtcagaa
      721 ccggcggcgt cgattccagc agggcatcgc caccggcggc gaattccggc ggatccgggc
      781 gcacatagtc gaacttgttc ttcttggcga accagccgat gtccagcgtg ctgaagccgg
      841 cgtccagatg ccgcgggtgc agatacatgt atccggcgcc cggtccgccg cgcaaatact
      901 tgtagctgcc gccaatcgcg aaatcggcat cgagcgcgcc gatgtcgacc ggcagcacgc
      961 cgagcgaatg atagacgtcg agcagcacgc gcgcgccgct ggagtgcgcc gcaccgataa
     1021 gcgcaggcaa cgccggcagc acctggccgg tgttgaacag cacctgggat acaacgacca
     1081 gatcgacgtc gcggatggcc gcgagaagct cgtcggcttc gaacattccg tcggcgcggg
     1141 cctcgacgaa ccggagctcg atgcgcttgc gaagcgcgta ttgcctcagg atcagatcga
     1201 tcgaatcgaa ttcgccgcgg gttgccacca cgcgcggcgg acgatcgtag ctgttcagga
     1261 tggcgcgcag cccctgtccc gcgctggtct tcggcacgac gcagtcggcg cgcggcgcgt
     1321 gcatcagcgc ggccagccgc gcccgatagg cggtgatctc cgccagccac gcgtcccacg
     1381 cgttgcccag ctgcgactgc cagagtccga cggcttcggc gacatcgtcc gccgtcgcgt
     1441 cgagaggccg gcccagcgag tgattggcga gatagaccgg ccgatcgggg tgcagcacgc
     1501 gcgagaacag cggcgcgacg tggcgcgcga tgccttcctc ggtcagcgga ccagggccca
     1561 tcgccgcgat cgccgccgcg atggtggccg cagtatcggc gcgcgggctc atgtgatcct
     1621 cccgcgctta cggctcggtt ggcaactgcg gccgcaagct gccccccgcc gggcggaatt
     1681 cgcgcctcgg agctccccgt cggtcctcat ggcgcgccga tcaccgtgcg cacatcgatc
     1741 agttcgggaa aaaacgtatg ctcgagcaca cggcgcaaga acgcgacgcc gctggatcct
     1801 ccagtgcctg tcttgtagcc gatgatgcgc tcgacggtct tcatatgacg gaagcgccag
     1861 agggcgaagg cctcgtcgac gtcgaccagt ttttccgcca tttcgtacgc gtcccagtac
     1921 tgctgcgggt tctcataaat cgtcttgaac acctcgacca gcgccgcgtt gcgctggtgc
     1981 gggaacgacc agtcgcgctc gacgcaggcg ggcggcaccg gcaatccgcg ccgcgacagg
     2041 tgacgcagga attcgtcgta gagcgacggc gcct
//
LOCUS       DATANS010000693         2475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_580372, whole genome shotgun sequence.
ACCESSION   DATANS010000693 DATANS010000000
VERSION     DATANS010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580372"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="VHT22_10345"
     CDS             complement(<1..1222)
                     /locus_tag="VHT22_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX3138000.1"
                     /translation="MTGAAAAAERGARSAAPGRRRRAWLLLATVALLVALPHVPGLQS
                     DFGRSLLTQMGIAAVFALSFNLLFGQTGLLSFGHAVYFGLGGYAAIGLMRAINHGLPL
                     PMLVVPLAGAAGGLLAGVLFGAVTTKRAGTIFALISLGVGELVYAATFMLPGIFGGEE
                     GITASRTRATIDFGFPLGSQLQVYYAVLLWTVAAALAMYAFTRTPVGRMCNAVRDNPE
                     RAEFVGYNTQRVRFIAFAMAGLFAGLAGGLHAINYEIVSADAVSAARSGAVLIMAYIG
                     GAAYFVGPILGAIVITWLQSSLSDYTSAWLLYLGVFFMAMILYAPSGLAGLIMMHRRI
                     AHPRALAGVLAAYAVALVPLALMAAGAVLAIEMAYRMSTQPELGTRMRLLWMSLDAAL
                     PWPWIVAAVALGGGL"
     gene            complement(1320..2270)
                     /locus_tag="VHT22_10350"
     CDS             complement(1320..2270)
                     /locus_tag="VHT22_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX3138001.1"
                     /translation="MLELVTVSLLNGVVYGLLLFMLSSGLTLIFSMMGVLNFAHASVF
                     MIGAYLSFQLGRWVGFWPALVIAPLLCGLAGAAIERYGLSRVHARGHVAEILFTFGLA
                     YLIEELVTMVWGRNAVANAVPAVIDFPLFHVFGSTFPAYKGLMMLISLAMFAFLYLAL
                     KKTRIGLVIQAALTHPKMLSALGHDVPGVFVLVFAVGSALAGLAGVIGGFMLLTQPTL
                     AHAIGPIVFVVVVVGGLGSLGGALLASMLIGILQTFAVAVDYSLLDLFRTLGIMISRG
                     SPLYELFAIKLATAAPIVPYLLLVLMLIVRPRGLLGTRDV"
BASE COUNT          505 a          849 c          804 g          317 t
ORIGIN      
        1 gcaatcctcc gccgagcgcc accgcggcga cgatccaggg ccagggcaaa gcggcatcga
       61 gcgacatcca tagcaggcgc atccgcgtgc cgagctcggg ctgcgtcgac attcgatagg
      121 ccatctcgat cgccaatacc gcgcctgccg ccatcaacgc aagcggcacc agcgcgacgg
      181 cgtatgcggc cagcacgccg gcgagcgctc tcgggtgggc gatccgccga tgcatcatga
      241 tcaggccggc gagcccggat ggcgcataca ggatcatcgc catgaagaac acgccgaggt
      301 agagcagcca tgccgacgtg tagtcggaga ggctgctctg cagccaggtg atgacgatcg
      361 cgcccaggat cgggccgacg aaatacgctg cgccgccgat gtacgccatg atcagcacgg
      421 cgcccgagcg cgccgcgctg accgcgtccg ccgagacgat ttcgtaattg atcgcatgca
      481 gcccgccggc gaggccggcg aagagtcccg ccatcgcgaa tgcgataaag cgcacgcgct
      541 gcgtgttgta gccgacgaat tccgcgcgct cgggattgtc gcgcaccgca ttgcacatgc
      601 gcccgacggg tgtgcgcgtg aacgcgtaca tcgccagcgc agcggcgacg gtccacagca
      661 ggactgcgta gtagacctga agctgcgacc ccaggggaaa cccgaagtcg atcgtcgcgc
      721 gcgtgcggct ggcggtgatg ccctcctcgc caccgaaaat cccgggcagc atgaacgtcg
      781 ccgcgtagac cagttcgccg acgccgagcg atatcagcgc gaatatcgtc cccgccctct
      841 tggtggtgac cgcgccgaag agcacgccgg cgagcaatcc gcccgccgca ccggccagag
      901 gcacgaccag catcggcagc ggcaatccgt ggttgatcgc gcgcatcagc ccgatcgcgg
      961 cgtagcctcc gaggccaaaa tatacggcgt ggccgaacga caacaggccg gtctggccga
     1021 acaacagatt gaacgacagc gcgaagaccg cggcgatccc catctgcgtg agcagcgagc
     1081 ggccgaaatc ggattgcagt ccgggaacat gcggcagcgc gacaagcagc gcgacagtcg
     1141 caagcagcag ccacgcgcgg cggcgacggc ctggcgcggc ggaccgggcc ccgcgctcgg
     1201 cggcggccgc cgctccggtc attgtacggc ccccacgctt gcgggctcat ttgccaatga
     1261 gcccacggcg ctgcccccaa aggtggcgca attcgcggct tgggacggcc cggcgccgct
     1321 catacgtcgc gcgtgccgag caggccccgc gggcggacga tcagcatcag cactagcagc
     1381 agatacggaa cgatgggcgc ggcggtggcg agcttgatcg cgaagagctc gtagagcggc
     1441 gagccgcgcg agatcatgat gccaagcgtg cgaaacagat cgagcagcga atagtcgacc
     1501 gccactgcga acgtctgcag gatgccgatc agcatcgacg cgagcagcgc cccgccgagc
     1561 gacccgaggc caccgacaac gacgacgacg aacacaatcg gcccgatcgc atgcgcgagc
     1621 gtcggctggg tcagcagcat gaagccgccg atcaccccgg cgagcccggc cagcgcgctg
     1681 ccaaccgcga acaccagcac gaacacgccc ggcacgtcgt gtcccaacgc gctcagcatc
     1741 ttcggatgcg tcaatgccgc ctggattacc agaccgatcc gcgtcttctt cagcgccagg
     1801 tagaggaagg cgaacatcgc cagcgagatc agcatcatca ggcccttgta cgccgggaac
     1861 gtgctgccga agacgtggaa cagcggaaag tcgatgaccg ccggcaccgc gttggccacg
     1921 gcgttgcgcc cccagaccat cgtcaccagc tcttcgatca ggtaggcgag tccgaaagtg
     1981 aacaggatct ccgcgacgtg gccgcgcgcg tggacgcgcg agagtccata gcgctcgatc
     2041 gccgcgccgg ccagcccgca caacaacggc gcgatcacca gcgccggcca gaaaccgacc
     2101 cagcggccaa gctgaaacga aagataggca ccaatcatga acacgctggc atgggcgaag
     2161 ttgagcacgc ccatcatgct gaagatcaag gtgagacccg acgacagcat gaacagcagc
     2221 agtccgtaga cgactccgtt cagcagcgag acagtgacga gctcgagcat gcgctcaagt
     2281 caaaggcgcc gcggcggcgg ccgcggcgcg cgttgcatca gcggaccgga cgcgccggct
     2341 cggtcacttc ggccgatcca tcttgcaagt cgtcggtagc gcagtcgcct tgccctcgac
     2401 cttggcgtcg gttttccagc cgagcccggt gccctcgctg tcgtacttga cgttccgggc
     2461 cagcgttgac acgta
//
LOCUS       DATANS010000694         1993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_619504, whole genome shotgun sequence.
ACCESSION   DATANS010000694 DATANS010000000
VERSION     DATANS010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619504"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1572)
                     /locus_tag="VHT22_10355"
     CDS             complement(<1..1572)
                     /locus_tag="VHT22_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3138002.1"
                     /translation="MPTQLPILAVRNLTTAFRVDDEWITVVKGISFEIGQRETVAIVG
                     ESGSGKSVTALSIMGLLPAANSRVSGEILLDGRDLLKLSENRMRHVRGNLISMIFQEP
                     MTSLNPVLTLGFQIAEALIYHRGLDRAQAEAETLRILERVRIPSAASRLHEYPHRLSG
                     GMRQRVMIAMALACKPKLLIADEPTTALDVTIQAEILELIKLLQDEEGMSVAFITHDM
                     GVVAEIADRTVVMLNGNAVEQAPTAELFASPRESYTRALLAAVPRLGSMKGRSAPQRF
                     AIVDPITGAVEVVPDTGSTAAMTERPVLEVAHLVTRFDIRSGVLSRVSSRVHAVEDIS
                     FSLHAGETLALVGESGCGKSTTGRSILRLVEPDSGTVLFEGQDVRMLDRAGLRAARQR
                     MQMIFQDPFASLNPRKTVGGAIAEPMIVHGLLSRADAHERVSELLRRVGLSPDMASRF
                     PHEFSGGQRQRLCIARALALAPRLLVADEAVSALDVSIKAQVINLLMDLQAEMGLAYL
                     FISHDIAVVERVSHRI"
     gene            complement(1596..>1993)
                     /locus_tag="VHT22_10360"
     CDS             complement(1596..>1993)
                     /locus_tag="VHT22_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3138003.1"
                     /translation="SIPPFARIARAPTIAVKEREFVDAGRTLGYSDTRLLVVHILPNI
                     LPEVLVMGSLWLANAIRTEASLAFVGLGVKPPTATWGGMIREGFENILDSYWLALVPG
                     VAILIVIFALNLLGDGLRDAIDPKLKGEA"
BASE COUNT          379 a          622 c          670 g          322 t
ORIGIN      
        1 gatgcggtgg ctcacgcgct cgaccaccgc gatgtcgtgc gagatgaaga ggtaggcaag
       61 gcccatctcc gcctgcaggt ccatgagcag gttgatgacc tgcgccttga tcgacacgtc
      121 gagcgccgac acggcttcgt cggcgacgag cagcctcggc gccagcgcca gcgcgcgtgc
      181 gatgcacagg cgctgccgct ggccgccgga gaattcatgc ggaaagcggc tcgccatgtc
      241 gggcgagaga ccgaccctcc ggagaagctc gctgacccgc tcgtgcgcat cggcacgact
      301 gagaaggccg tgcacgatca tcggttcggc gatcgcgccg ccaacggtct tgcgcggatt
      361 gaggctcgcg aacggatcct ggaaaatcat ttgcatgcgt tggcgagcgg cgcgcaagcc
      421 cgcgcggtcg agcatgcgaa catcctgtcc ctcgaacagg acagttccgg aatcgggctc
      481 gaccaggcga aggatcgagc ggcccgtggt cgacttgccg cagccgctct cgccgaccag
      541 cgccagcgtc tcgccggcgt gcaacgaaaa tgaaatatcc tcgacggcgt gaacccgtga
      601 gctgacgcga ctcagcacgc cggagcgaat gtcgaaacgg gtgacaaggt gggcgacttc
      661 gagcaccggg cgctcggtca tcgccgcggt cgaaccggta tcggggacga cttcgaccgc
      721 gcctgtgatc ggatcgacaa tcgcgaatcg ctgcggggca gagcggccct tcatcgagcc
      781 gaggcgcgga accgcggcga gcagtgcgcg ggtataagac tcccggggcg atgcaaaaag
      841 ctctgcggtg ggtgcctgct cgacggcgtt gccattcagc atgacgaccg tgcgatcggc
      901 gatctcggcc accacaccca tgtcatgggt gatgaaggcg accgacatgc cctcctcgtc
      961 ctgcagcagc ttgatcagct cgaggatttc ggcctggatc gtgacgtcga gcgcggtcgt
     1021 cggttcgtcc gcgatcagca gcttcggctt gcaggcgagc gccatggcga tcatcacgcg
     1081 ctgccgcatg ccaccggaga gccggtgcgg atactcgtgc aagcgcgacg cggcggaggg
     1141 gatgcgcacg cgctccagga tgcgcagcgt ttcggcctcc gcctgcgcgc gatcgaggcc
     1201 acgatggtag atcaaggctt cggcgatctg gaagccgagg gtcaataccg ggttgaggct
     1261 ggtcatcggc tcctgaaaga tcatcgaaat caggttgccg cgcacatgcc gcatccggtt
     1321 ttcggatagt ttgagcagat cgcgaccgtc aagcagaatt tcgcccgaca cgcggctgtt
     1381 ggctgccggc agcagcccca tgatcgagag cgcggtaacg ctcttgcctg aacccgattc
     1441 gccgacgatt gccaccgtct cgcgctgccc gatctcgaag gaaatcccct ttacgacggt
     1501 gatccactcg tcgtcgaccc ggaacgcggt cgtcaggttg cgtacggcca ggattggcaa
     1561 ctgagttggc aactgagccg gcgccgcagc ggacattagg cttcgccctt cagtttcgga
     1621 tcgatcgcgt cgcgcaagcc gtcgccaaga aggttgagcg cgaagatgac gatcagaatc
     1681 gcaacaccgg ggaccagcgc cagccagtag ctgtcgagaa tgttctcgaa gccttcgcgg
     1741 atcatgccgc cccaggtcgc agtcggcggc ttgacgccaa gtccgacgaa cgccagagag
     1801 gcttccgtgc gaatggcgtt ggcaagccag agcgagccca tcaccaggac ttcgggcagg
     1861 atgttcggca ggatatggac gacaagcagc cgcgtgtcgg agtagccgag cgtgcggccg
     1921 gcgtcgacga actcgcgctc cttgaccgcg atcgtcggcg cgcgggcaat gcgggcaaaa
     1981 ggcgggatcg aag
//
LOCUS       DATANS010000695         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_33764, whole genome shotgun sequence.
ACCESSION   DATANS010000695 DATANS010000000
VERSION     DATANS010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33764"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VHT22_10365"
     CDS             complement(<1..678)
                     /locus_tag="VHT22_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3138004.1"
                     /translation="MAVRKPAPGDLEPIETASRDELQSLQLQRLQWSLAHAYDHVPHY
                     RNAFDHAGVHPRDCRSLADLARFPFTVKSDLRDNYPFGMFAVPREQVVRIHASSGTTG
                     KPTVVGYTRSDIDTWANVMARSIRAAGGRAGDIIHVSYGYGLFTGGLGAHYGAERLGA
                     TVIPMSGGQTEKQVQLIQDFKPNIIMVTPSYMLAIAEEMERQNIDPRKTSLEIGIFGA
                     EPWTATMR"
     gene            complement(710..1180)
                     /gene="paaI"
                     /locus_tag="VHT22_10370"
     CDS             complement(710..1180)
                     /gene="paaI"
                     /locus_tag="VHT22_10370"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HEX3138005.1"
                     /translation="MGEHHPAASPAEAQALAERVASAMYERDRASQALGMHIVRVGAG
                     HAELSMRVRADMLNGHLTCHGGFIFSLADSAFAFACNSYNLTTVASGCTIEFVAPARE
                     NDVLTAVAQERSVSGRTGVYDIEVNNQDGAAIAFFRGKSYRIKGHVLDGADRTA"
     gene            complement(1198..1989)
                     /gene="paaG"
                     /locus_tag="VHT22_10375"
     CDS             complement(1198..1989)
                     /gene="paaG"
                     /locus_tag="VHT22_10375"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HEX3138006.1"
                     /translation="MSYGNILFELAGGIARLTLNRPDRLNSFNDAMHAEVRDAVGRVR
                     ADPSVRVLLLTGAGRGFCAGQDLGDRAVAPGADPVDLGASIDRNYKPLVLALRALPLP
                     VVCAVNGVAAGAGANIALACDIVIAAKSASFVQAFSKIGLIPDSGGTYLLPRLIGTAR
                     AMGLAVFGDKLPAEQAASWGLIWRCVDDAELPAAVDAILKQLASAPTRGLAATKQAIY
                     AAPGNTLEAQLDLERDLQRELGTSADYREGVAAFAAKRPPQFVGK"
BASE COUNT          339 a          693 c          686 g          347 t
ORIGIN      
        1 gcgcatcgtc gccgtccatg gctcggcgcc gaaaatacca atttcgagcg atgtcttcct
       61 cgggtcgatg ttctggcgct ccatttcctc ggcgatcgcc agcatgtacg acggcgttac
      121 catgatgatg ttgggcttga aatcctggat cagctgcacc tgcttttcgg tctggccccc
      181 cgacatgggg atgaccgttg cgcccagcct ctccgcgcca tagtgggcgc ccagcccacc
      241 ggtgaacagg ccgtacccgt aggagacgtg aatgatgtcg ccggcgcgcc cgcccgcggc
      301 gcgaatcgag cgggccatta cgttcgccca ggtatcgatg tcggagcgcg tgtagccgac
      361 aaccgtcggt ttgccggtcg tacccgagga ggcatggatg cgaacgacct gttcgcgcgg
      421 taccgcgaac atcccgaatg ggtagttgtc gcgaaggtcg gatttgacgg taaacggaaa
      481 gcgcgcgagg tcggcgagcg agcggcaatc gcgcggatgc acgccggcgt ggtcaaacgc
      541 gttgcggtaa tgcgggacgt ggtcgtaggc gtgcgcaagc gaccattgca ggcgctgcaa
      601 ctgcaatgac tgcaactcgt cgcgactggc ggtctcaatc ggctcgagat ccccgggggc
      661 gggcttcctg acggccatta atctcctcct gctcctgcgc cggcgcggac taggcggtcc
      721 tgtcggcgcc gtcgagcacg tgacccttga tccgatacga cttgccacgg aaaaaagcga
      781 tcgcggcgcc gtcctggttg ttcacctcga tatcgtatac gccggtgcgg ccgctgacgc
      841 tgcgctcctg ggcgaccgcg gtgagtacgt cgttctcgcg cgccggtgcg acgaattcga
      901 tcgtgcagcc ggaagcgacc gtcgtcaggt tgtagctgtt gcaagcgaag gcgaatgcgc
      961 tgtcggccag cgaaaaaatg aacccgccgt ggcaggtgag gtggccgttg agcatgtcgg
     1021 cgcgcacgcg catcgaaagc tcggcatgac cagcgccgac gcgcacgatg tgcatgccca
     1081 gcgcttgcga agcccggtca cgctcgtaca tcgccgacgc cacgcgctcg gccagcgcct
     1141 gcgcctcggc gggactcgcg gccggatgat gctcgcccat cgacgccagg ccctccgcta
     1201 ttttccgacg aactgcggcg gacgcttcgc cgcgaacgcg gcgacgcctt cgcgatagtc
     1261 ggcgcttgtg ccgagctcgc gctgcagatc gcgctcgagg tcaagctgcg cttcgagcgt
     1321 gttgccgggc gcagcataaa tcgcctgctt ggtcgcggcg agcccgcgcg tcggcgcgct
     1381 cgccaattgc ttcaatatcg cgtcgacggc cgccggcaac tctgcgtcgt cgacgcagcg
     1441 ccagatcagt ccccagctcg ccgcctgctc ggccgggagc ttgtcgccga acaccgcgag
     1501 ccccatcgcc cgcgcggttc cgatgagccg cggcagcaga tatgttccgc cggaatccgg
     1561 gatcaagccg atcttgctga acgcctgcac gaaactcgcc gatttcgcgg cgatgacaat
     1621 gtcgcaggcg agtgcgatat tggctcccgc gccggcggcg acgccgttga ccgcgcaaac
     1681 gaccggcaac ggcagcgcgc gcagcgccag caccagcggt ttgtaattgc gatcgatcga
     1741 cgcgcccaga tcgaccgggt cggcgccggg cgccactgcg cggtcgccga ggtcctgccc
     1801 ggcgcaaaaa ccgcgtcccg cgccggtcag cagcagcacc cgcaccgacg gatccgcgcg
     1861 gacgcggccg accgcgtcgc gcacctcggc gtgcatcgca tcgttgaagc tgttgagccg
     1921 atcgggccga ttgagcgtca ggcgcgcgat gccgccggcg agctcgaaca gtatgttacc
     1981 gtagctcatt gcggtgccgc cgcgacgtgc ttccggtgct gcacgacccg gaatcgtccg
     2041 gcgacgaacg ccgaatcggt cagcg
//
LOCUS       DATANS010000696         3511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_619636, whole genome shotgun sequence.
ACCESSION   DATANS010000696 DATANS010000000
VERSION     DATANS010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619636"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VHT22_10380"
     CDS             <1..269
                     /locus_tag="VHT22_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX3138007.1"
                     /translation="SIHPTVLHAACRSARVAAGLDKRVSVHTLRHSFATHLLESGTDI
                     RIIQALLGHSSLHTTARYTQVATSTIRGTPSPLDRLRLEVTPPS"
     gene            279..1466
                     /locus_tag="VHT22_10385"
     CDS             279..1466
                     /locus_tag="VHT22_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HEX3138008.1"
                     /translation="MRPALEVADIFRRHGDAFRQTHHLHLGRVERRVMAAIEACRTPA
                     LGGHVEQCADCGLVRCAYNSCRNRHCPKCQGLARAAWLDARQAELLPVPYFHVVFTLP
                     VPAAEIAFQNKRAVYAILFRAAAEALRDIAADPRHLGAEIGAVAVLHTWGQSLHHHPH
                     LHCIVPGGGLSPDQTRWIACRPGFFLPMRVLSRRFRTLFLAQLQVAFAAGELRFSGAL
                     AALTEPEAFAARLGAMRGTEWVVYAKPPFAGPKQVLAYLGRYTHRVAIANSRLTQLSD
                     SQVSFTWKDYRHHGKTKVMTLAADEFIRRFLQHTVPGGFHRIRHVGFLANGHRMAKLA
                     LCRALLDAPTPEPPQQQPWRDRYRQLTGEAIEVCPDCGGEMREQGVLLRRPSRGPFWC
                     DTS"
     gene            1847..3424
                     /locus_tag="VHT22_10390"
     CDS             1847..3424
                     /locus_tag="VHT22_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3138009.1"
                     /translation="MSPSSDLARLPRAELEALVVKLLGEVAELKRVVAEQRDEIARLK
                     GLKSRPDIKPSGMEDATRPKPPRRGKHRRRGKSAPRVRVESRVLRVDVPPGSRFKGYD
                     SFVVQDLVLRAQVIRYRRERWVTPDGRTVVASLPQGVVGHFGPELRRFVLLQHHQGQV
                     TVERLTAQLRAVGLSISKRQVVRLLIDRQDGFLAENRDVLRAGLQTAGWITVDDTGAR
                     HAGRHGFCTQIGNDAFTWFGTRASKSRLNFLDLLRAGHGDYVVNETALAYMRARSLAG
                     PVLSRLAAEPQTRFSDHTAWQAHLQRLGIDALHVTPDPVSIATEGALWGAVAAHGFLQ
                     NAVIVSDDAGQFAVGQHALCWVHAERLVHKLDTFTDLHRAAQARMRELIWWFYADLKA
                     YRSAPTARRRSEMRARFDRILDRRTGFATLDRLLERLHANKPELLMVLDRPEIPLHTN
                     GSERDIRCHVTKRKISGGTRSDAGRDCRDAFLGLMHTCAKLGIAFWDYLADRLGIIGH
                     PSVPALPHLIRCRGQPA"
BASE COUNT          565 a         1248 c         1119 g          579 t
ORIGIN      
        1 attcgatcca tccgaccgtg ctgcatgccg cctgccggtc ggcgcgtgtc gccgccgggc
       61 tcgacaagcg ggtgagcgtg cacacgctgc ggcacagctt cgccacccat ctgctcgaga
      121 gcggcaccga catccgcatc atccaggccc tgctcggcca cagcagcctg cacaccacgg
      181 cgcgctacac ccaggtcgcg accagcacga tccgcggcac gccgagcccg ctcgatcgtc
      241 tgcggctgga ggtgacgcct ccgtcctgag acggccgcat gcgccccgcg ctagaggtgg
      301 cggacatctt ccgccgccac ggcgacgcgt tccggcagac gcaccacctg catctcggcc
      361 gcgtcgaacg tcgcgtcatg gcggcgatcg aggcgtgccg cacccctgca ctcggcggcc
      421 atgtcgagca gtgcgctgat tgcggcctgg tccgttgcgc ctacaactcg tgccgcaacc
      481 gccattgtcc gaagtgccag ggcctggcgc gcgccgcctg gctcgacgcg cgtcaggccg
      541 agttgctgcc ggtgccatac ttccatgtcg tgttcaccct gccggtgccg gcggcggaga
      601 tcgcgttcca gaacaagcgc gcggtctacg ctatcctgtt ccgcgcggcg gccgaggcat
      661 tgcgcgacat cgccgccgat ccgcgccatc tcggcgctga gatcggcgcg gtggcggtgc
      721 tgcacacatg gggacaatcc ctgcaccacc atccgcacct gcactgcatc gttccaggcg
      781 gcggactctc gcctgaccag acgcgatgga tcgcttgccg accgggattc ttcctgccca
      841 tgcgggtgct ctcgcgccgg ttccgcacac tattcctggc ccagctgcag gtggcatttg
      901 cagcgggtga gctgcgcttc tctggcgcgc tcgccgcact caccgaaccg gaagcattcg
      961 ccgcgcgcct cggcgcaatg cgtggcacgg agtgggtggt ttacgccaag cctccgtttg
     1021 ccggccccaa gcaggtactc gcctatctcg gccgctacac ccatcgcgtc gccatcgcca
     1081 acagccggtt gacccagctc agcgacagcc aggtcagctt cacctggaag gactaccgcc
     1141 atcacggcaa gaccaaggtg atgacacttg ctgccgacga gttcatacgg cggttcctgc
     1201 agcacaccgt cccgggcggg ttccaccgca tccgacatgt tggctttctc gccaacggcc
     1261 atcgcatggc caaactcgcg ctctgccgag ccctgcttga tgcaccaacg ccggagccac
     1321 cgcagcagca gccctggcgc gaccgctatc gtcagctgac cggcgaggca atcgaagtct
     1381 gcccggactg cggcggcgag atgcgggagc agggcgtgct gttgcgccgt ccgtctcgcg
     1441 gaccgttctg gtgcgacact tcataaccgc cgcgatccgg acggtcgcat cgctccaagc
     1501 ccaattcgcc gacatcatcg ccggcatggg taggggcgcg ctgatcaaca acaagacgcc
     1561 ggtggtgacc tcggctgcat gtcaaatccg ccgcgtcgca ctgccattgc cagctaccgt
     1621 ggccccgatg atgcttactg ctccatgggg tcaaccggtc cgagtccact gcgtgcagca
     1681 ccgggtcgtg ccgacagtcg cactacaccc atagccgtcg gcccgcggtt cagttcaatc
     1741 cggcttcaat gaggtcgcgc ccatgcactt cgccgcgacc gtcccgcgcg acctcacaga
     1801 agcctccaga ttcccccgac gcggaggagt acgattcgct ccgggcatgt ccccttccag
     1861 cgatctcgcg cggcttccgc gggccgaact cgaagctctg gtggtcaaac tgctcggcga
     1921 ggttgctgag ctgaagcggg tggtagcgga gcagcgcgac gagatcgcac gactgaaagg
     1981 tctgaaaagc cgccccgaca tcaagccgag cggcatggag gatgcgacgc ggccgaagcc
     2041 gccgcgccgg ggcaagcatc gtcggcgcgg caagtccgca ccgcgggtca gggtcgagag
     2101 ccgggtgcta cgcgtggatg tgccgccggg ctcgcggttc aagggctacg actccttcgt
     2161 cgtccaggac ctggtgctgc gcgcgcaggt gatccgctat cggcgggaac gctgggtaac
     2221 gccggacggc cgcacggtgg tggcatcact gccgcagggc gtcgttggcc atttcgggcc
     2281 ggagctgcgc cgctttgtgc tgttacagca ccatcagggc caggtaacgg tcgagcggct
     2341 gaccgcgcag ctgcgcgccg tgggtcttag catctccaag cgccaagtgg tgcggctgct
     2401 gatcgatcgc caggacgggt tcctggccga aaaccgtgac gtgctgcggg ctggactgca
     2461 gaccgctggc tggatcaccg tggacgacac cggggcacgg cacgccggcc gtcatggctt
     2521 ctgcacgcag atcggcaacg acgccttcac ctggttcggc acgcgggcga gcaagagccg
     2581 gctcaacttc ctcgacctgc tgcgcgccgg ccacggcgac tatgtggtca acgagacggc
     2641 cctggcctac atgcgggccc ggtcgctcgc cgggccggtg ctcagccgtc tggcagccga
     2701 gccgcaaaca cgcttctccg accacacggc ctggcaggcc catctgcagc gtctcggcat
     2761 cgatgccttg cacgtcacgc ccgacccggt ctcgatcgcg accgaaggcg cgctctgggg
     2821 cgcggtggcg gcgcacggct ttctgcagaa cgcggtgatt gtcagcgacg acgcgggcca
     2881 gtttgcggtc gggcagcacg cgctgtgctg ggtgcacgcc gaacggctgg tgcacaagct
     2941 cgacaccttc accgatctcc accgcgccgc ccaggcgcgc atgcgcgagc tgatctggtg
     3001 gttctacgcc gatctcaagg cctatcgcag cgcaccgacg gcccggcgcc gcagcgagat
     3061 gcgcgcccgt ttcgaccgca tcttggaccg acgcaccgga ttcgccaccc tcgatcgcct
     3121 gctcgagcgg ctgcatgcca acaaacccga gttgctgatg gtgctcgatc ggccggagat
     3181 cccgctgcac accaacggtt cggagcgcga catccgctgc cacgttacca agcgtaagat
     3241 cagcggcgga acccgcagcg atgccgggcg tgattgccgc gacgcgtttc tcgggctcat
     3301 gcacacctgc gccaagctgg gtatcgcgtt ctgggattat ctcgcagacc ggctcggcat
     3361 catcgggcat ccaagcgtgc ccgcactccc gcatctcatc cgctgccggg gacagccagc
     3421 ctaaaccctg tcgcccggac ttttgccccg cttactgcga gagctgcaaa ggcattgaaa
     3481 atgtgcacga agtggaatcc ttgggtcgcc t
//
LOCUS       DATANS010000697         1838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_455964, whole genome shotgun sequence.
ACCESSION   DATANS010000697 DATANS010000000
VERSION     DATANS010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455964"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VHT22_10395"
     CDS             complement(<1..321)
                     /locus_tag="VHT22_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005216288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX3138010.1"
                     /translation="MTDSWLLYGANGYTGELIAREAVARGMRPTLAGRSRQKVEQLAA
                     ELGCQSAVFSLDDHTSLVRALDGMTAVLHCAGPFSSTARPMMQGCLATHVHYLDITGE
                     IEVFE"
     gene            complement(318..1652)
                     /locus_tag="VHT22_10400"
     CDS             complement(318..1652)
                     /locus_tag="VHT22_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3138011.1"
                     /translation="MTKRALIVDDSRSARVILSRMLEGYGLQVDSSESAEHALEFLRQ
                     ARPDVIFMDHLMPGMDGFQAIQAIKGNPDTAMIPVVMYTSQEGELYVSQARALGAVGV
                     LPKTVKQADVSRVLYQLRLLPERRDARPASPVYSAQESAAVRAQPQAVALGDIETAIR
                     NATGPVLKEHNTEMRRFVLASLEAFARRISTEMKPAAAPTPAPEPIEAPPPPPQSRWP
                     LVAAIAALAVLPTFVLAVIHTRTLDALNELAQQNTKLAQFSQEQQLQLDALRKAVQGR
                     EIQLATTAAARPADADVESEPVPYGEPPLSGARLERMRDMIGQLKAGGFHGTIKVATY
                     VGEFCLVGNGIEGYSMAADDLPASRCDLRGNPFDDGLSAAQRQSLPFANLVASLRQDK
                     SEQLRVEVEHEGRKPAVPYPRGDQLAKVTAGDWNKIAAQNNRVEFAAQPAGS"
BASE COUNT          299 a          591 c          623 g          325 t
ORIGIN      
        1 ctcgaagact tcgatctcac cggtgatgtc gagatagtga acgtgcgtgg cgagacagcc
       61 ttgcatcatc ggccgtgccg tcgaggagaa cggtcccgcg caatgcagga cagcagtcat
      121 gccatcgagt gcacgaacca gcgaggtgtg atcgtcgagc gagaacactg cgctctggca
      181 accgagctcg gctgcgagct gctcgacctt ctgccgcgag cgaccggcga gcgtgggccg
      241 catgccgcgg gcaaccgcct cgcgtgcgat cagttccccg gtgtagccgt tcgccccata
      301 gagcagccaa ctatcggtca tgaccccgcc ggctgcgcgg caaactcgac gcggttgttc
      361 tgggcggcga tcttgttcca gtcaccggcc gtgactttcg ccagctggtc gccgcgcgga
      421 tacggcaccg ctggcttgcg accttcgtgc tcgacttcga cgcgcagctg ctcgctttta
      481 tcctgacgca gcgacgcgac cagattcgca aacggcagcg actgacgctg cgcggcgctc
      541 aggccgtcgt cgaacggatt gccgcgcagg tcgcagcggc tcgccggcag atcgtccgcg
      601 gccatcgagt agccttcgat gccgttgccg accaggcaga actcgccgac gtacgtcgcg
      661 accttgatgg tgccgtgaaa gccgccggcc ttgagctgcc cgatcatgtc gcgcatgcgt
      721 tcgagacgcg caccggacaa cggtggctcg ccgtaaggca cgggttcgga ttcgacgtcc
      781 gcgtcggcag gtcgtgccgc tgccgtcgtg gccagctgga tctcgcgacc ctgtacggcc
      841 ttgcgcaaag catccagctg caactgctgt tcctgactga actgcgcgag cttggtgttc
      901 tgttgcgcga gctcgttcag cgcgtcgagt gtccgggtat ggatgacagc gagcacgaac
      961 gtcggcagca ccgcgagcgc cgcgattgcg gcgaccagcg gccagcggct ctgcggcggc
     1021 ggcggcggtg cttcgatcgg ctcgggtgcc ggcgtcggcg cagccgcagg tttcatctcg
     1081 gtgctgatgc ggcgtgcaaa tgcctccagg ctcgcgagca cgaagcgacg catctccgtg
     1141 ttgtgctctt tgagcaccgg tccggtcgcg ttccggatcg cagtctcgat gtcgccgagc
     1201 gcgacggctt gcggctgggc gcgaacggca gcggattcct gcgcactgta gacgggtgac
     1261 gccgggcggg cgtcgcgacg ctcgggcaac agccgcagct ggtacaggac ccgcgacaca
     1321 tccgcctgct tgacggtctt cggcagcacg ccgaccgcgc cgagcgcgcg cgcctggctg
     1381 acgtacagct cgccttcctg cgaggtgtac ataacgaccg ggatcatcgc cgtgtccgga
     1441 ttgcccttga tcgcctggat ggcctgaaag ccgtccatgc ccggcatcag gtgatccatg
     1501 aagatcacgt ccgggcgggc ttgccgcaaa aattccaggg catgctccgc gctctcggag
     1561 gaatcgactt gcagcccgta accttccagc atgcggctga ggatgacgcg ggccgagcgc
     1621 gaatcatcga cgatcagggc tcgcttggtc atagcgcggg gatgatagtc gcaggggtca
     1681 ttaccgacaa cgcggggcgg ttccgaggag tatgctcagg tcaaacaccg cgcgcgcagg
     1741 gggatggctt ccatggcgta ctcgacctcg gatatccgga atatttcgct ggtggggcag
     1801 gcgggggccg gcaagacctt gctggccgaa agcctgct
//
LOCUS       DATANS010000698         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_242599, whole genome shotgun sequence.
ACCESSION   DATANS010000698 DATANS010000000
VERSION     DATANS010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242599"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VHT22_10405"
     CDS             complement(<1..666)
                     /locus_tag="VHT22_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138012.1"
                     /translation="MTMARASRWAIALWAGALVGCAIVIARTEFTADMSAFLPRSPTP
                     VQQILVQQLRDGVVSRLILIAVDGGIPAALAQTSMRMAAELRKHPAEFAAVNNGEDSA
                     LDLDREFLWRNRYLLSPAVAPGHFSAPALRDALEENLQLIGSPAGALLRRILPQDPTG
                     ELLRLIAQFEGVARPEVREGVWFSPDGSRALLVAQTRAAGYDIDAQERALNLIRAAFA
                     GGGA"
     gene            complement(663..1229)
                     /locus_tag="VHT22_10410"
     CDS             complement(663..1229)
                     /locus_tag="VHT22_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX3138013.1"
                     /translation="MFVFALAAGGAAVAAETPVWDIEQLMQGLSKVPAAKGKFVERKY
                     LAMLTAPLDSSGTLVYTAPGRLEKNTLAPKPERLVLDQDKLTLEYPDRGQRRVLALQN
                     YPVVWAFVESIRSTLAGDYQTLNRFYRASVAGGEEQWRLSLKPVDPKMQAVVQEIRIG
                     GSHNRVRTIEILETQGDRSVMTITEDGP"
     gene            complement(1249..>1521)
                     /locus_tag="VHT22_10415"
     CDS             complement(1249..>1521)
                     /locus_tag="VHT22_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX3138014.1"
                     /translation="FRFLGEAAHFPTGPFRVAAMLQRPLVLMFGLYRGGNRYEIHFER
                     LVDAWPASRVERKRTVAEAQREYVTRLEHHCRDAPYNWYNFYDFWK"
BASE COUNT          263 a          494 c          509 g          255 t
ORIGIN      
        1 agcgccgccg ccggcgaatg ccgcacggat cagattcagc gcgcgttcct gcgcgtcgat
       61 gtcgtatccg gcggcgcggg tttgcgcgac gagcaaggcg cgactgccgt cgggcgagaa
      121 ccacacgcct tcgcgcacct cgggccgcgc gacgccttcg aattgcgcga tcagccgcaa
      181 cagttcgccg gtggggtctt gcggcaggat ccggcgcaac agcgcgccgg ccggcgagcc
      241 gatgagttgc aggttttcct cgagcgcatc gcgcagcgcg ggcgcggaaa aatgcccggg
      301 cgcaaccgcc gggctcagca ggtagcggtt gcgccacaga aattcgcggt ccaggtcgag
      361 cgcgctgtcc tcgccgttat tgaccgccgc gaactcggcc gggtgcttgc gcagttccgc
      421 cgccattcgc atgctggttt gcgcgagtgc cgcgggtatg ccgccgtcga ccgcgatgag
      481 gatgagcctc gaaaccacgc catctcgcaa ttgctgcacg agtatctgct gcacgggcgt
      541 cggcgagcgc ggcaagaaag cggacatgtc ggcggtaaat tcggtgcgcg caatcacgat
      601 ggcgcagccc accagcgcgc ctgcccagag cgcgatcgcc cagcgcgacg cgcgcgccat
      661 cgtcatgggc cgtcctcggt gatcgtcatg accgagcggt cgccctgcgt ttcgaggatt
      721 tcgatggtgc gcacccggtt gtgcgacccg ccgatgcgga tttcctgcac gaccgcttgc
      781 attttgggat cgaccggctt gagcgacagg cgccactgct cctcgccgcc ggcaacgctc
      841 gcgcggtaga accggttcag tgtctggtaa tcgcctgcaa gggtcgaacg gatactctca
      901 acgaaggccc acacgaccgg gtaattctgc agcgcgagaa cccggcgctg gccgcggtcc
      961 ggatattcga gagtcagttt gtcctgatca agcaccagcc gctcgggctt gggcgccagc
     1021 gtgttttttt cgaggcggcc cggcgcggta tagacgagtg tgccggagga gtcgagcggt
     1081 gcggtgagca tggcgagata tttgcgctcg acgaatttgc ctttcgccgc ggggaccttg
     1141 ctaagtcctt gcatgagctg ctcgatatcc cacacgggcg tctcggctgc aacggcggcg
     1201 ccgccggccg ccagcgcgaa cacgaacatc gcgctgcgcc agcgactcct atttccagaa
     1261 atcataaaag ttgtaccagt tgtacggcgc gtcgcggcag tgatgctcca gacgcgtcac
     1321 atattcgcgc tgcgcctcgg caacggtgcg cttgcgctcg acgcgcgaag cgggccacgc
     1381 atcgaccagg cgttcaaaat gaatctcgta gcggttgccg ccgcgataga ggccgaacat
     1441 cagcaccagc ggccgctgca gcatggcggc gacgcggaac gggccggtcg gaaaatgcgc
     1501 ggcctcgccc aaaaaccgga a
//
LOCUS       DATANS010000699         1686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_750258, whole genome shotgun sequence.
ACCESSION   DATANS010000699 DATANS010000000
VERSION     DATANS010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750258"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..877
                     /locus_tag="VHT22_10420"
     CDS             191..877
                     /locus_tag="VHT22_10420"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEX3138015.1"
                     /translation="MTEPAKLDEPLADADYDELEAFLASNGVPQDCMDLEMLDGFLTA
                     IVSGPEMIQPSEWLPVVWSDSQRSVSPVFADNEQAGRILSLVLRVQNSIARTLRESPT
                     RFKPLLYHSEDNVKGDKAPPEASAWCEGYMTGVLLREAEWEPMYSQEDARDWMLPIEA
                     LAYGDHDPEYLDWVDSEEKRQGMIDELPIAAVLIYRYWQERNRPESANRAERRAQRRE
                     TSRKGRQRLH"
     gene            complement(957..1232)
                     /locus_tag="VHT22_10425"
     CDS             complement(957..1232)
                     /locus_tag="VHT22_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138016.1"
                     /translation="MNHRATRVQEAASFAAGALIGTSIVTPVFAATSDLPAEWQPFLL
                     IGSVVLLAMALMLKALGTRPSRRGDLDESADLREANRVEMIDVPLHQ"
     gene            complement(1370..>1686)
                     /locus_tag="VHT22_10430"
     CDS             complement(1370..>1686)
                     /locus_tag="VHT22_10430"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEX3138017.1"
                     /translation="VIGSGHTTHNLRDWMSNPRRQEPLRYAKAFAEWLNDRLIAHDVD
                     ALVAWREQAPEAARAHPSDEHFLPLFVALGAAGDNAEVERFVQGFEAGALAMDSYRFG
                     PH"
BASE COUNT          319 a          545 c          544 g          278 t
ORIGIN      
        1 gctcgccgca gcgcatcagg ccggcgccgt gttgttgttc gccgcggcgc tcaatgccgc
       61 gtatgcgctg cgcattcgcg ggcgtggcca ggaagaacgc gcatgagcaa gtccggaacg
      121 cgcgggcacg catttgcatt ctgcctcccc agggaggatc atgacggccc gatgcgcaaa
      181 cgagtccacc atgactgagc ccgccaagct cgacgaaccg ctggccgacg ccgattacga
      241 cgagctcgaa gccttcctgg catcgaacgg tgtaccgcag gactgcatgg acctggaaat
      301 gctcgacggc tttttgacgg cgatcgtcag cgggccggag atgatccagc cgtccgaatg
      361 gctgcctgta gtctggagcg acagtcagcg cagcgtctcg ccggtcttcg ccgacaacga
      421 gcaggcgggc cggatactgt cgctggtcct gcgcgtgcag aacagcatcg cgcggacctt
      481 gcgcgaatcg ccgacccgct tcaagccgct gctatatcac agcgaagaca acgtgaaggg
      541 cgacaaggcg ccgcccgaag cgagtgcgtg gtgcgaaggc tacatgaccg gcgtgctgct
      601 gcgcgaggcg gaatgggagc cgatgtattc gcaggaagac gcccgcgact ggatgctgcc
      661 gatcgaggcg ctggcctacg gcgatcacga tcccgaatac ctggattggg tcgacagcga
      721 ggaaaagcgg caaggcatga ttgacgagct gccgatcgcc gccgtgctca tctatcgtta
      781 ctggcaggag cgcaaccggc ccgaatcggc caatcgcgcc gagcggcgcg cgcagcggcg
      841 ggagacctca cgaaaaggcc gccagcgact gcactgacgg cctcttcagg ccggccgcgg
      901 cacggccggc tttcccgcgt ggtgcgagga ttccgtacta cttactgatc tcgtacttac
      961 tgatgcagag gcacgtcgat catctcgacg cgattcgctt cgcgcaggtc ggcgctttca
     1021 tccagatcgc cgcgccggct cggccgcgta cccagggcct tgagcatcaa ggccatggcc
     1081 agcaggacca ccgatccgat cagcaggaag ggttgccatt cggcaggaag atcggatgtc
     1141 gccgcgaata ctggcgtcac gatcgatgtg ccgatcaagg cgcccgcggc gaacgacgcc
     1201 gcctcttgca ctctggtcgc tcgatggttc atctgtgact ccgcttcgat tgattgaaca
     1261 cggcttcact ttaggaccac gccctcggcg ccgtaatcgg cgcgcatccg atttatgcgt
     1321 aagacgtgct ccgacgcggc ggcgaaacgt cgtcgctgag cgacaaaaat cagtgcgggc
     1381 cgaagcggta ggagtccatc gcgagtgcgc cggcctcgaa gccttggaca aagcgctcga
     1441 cctccgcatt gtcgcccgcg gcgcccagcg caacgaacag cggcaaaaaa tgctcgtcgc
     1501 tcggatgcgc gcgcgccgcc tccggcgcct gctcgcgcca ggcgaccagc gcgtcgacgt
     1561 catgcgcgat tagccgatca ttcagccatt cggcgaacgc ctttgcataa cgcagcggct
     1621 cctgccggcg cggattggac atccagtcgc gcaggttgtg cgtggtgtga cccgaaccga
     1681 tgacca
//
LOCUS       DATANS010000700         2199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_242815, whole genome shotgun sequence.
ACCESSION   DATANS010000700 DATANS010000000
VERSION     DATANS010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242815"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..870)
                     /locus_tag="VHT22_10435"
     CDS             complement(100..870)
                     /locus_tag="VHT22_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX3138018.1"
                     /translation="MPLQAVEPRRLYRQIAEQIAQLIASGEFLPGSRLPAERELAASL
                     GVSRASVREAIISLEISGLVEVRVGTGIFVAASAVPRQSAAADEGPGPFELLDARKLV
                     EGEIAALAAVSSQPSDLEALRQSVRAMEAGIRDFALREAIDREFHLLIAKATRNGSLE
                     LVVAGLWDQRAELWGRMQQHFHTPELALKTIEDHDAIVGAIASRDAEGARAAMHRHLA
                     RVAREFQRGVDGSRRVTTPRKPARPRAAAKPRQPRPRP"
     gene            1198..>2199
                     /gene="manD"
                     /locus_tag="VHT22_10440"
     CDS             1198..>2199
                     /gene="manD"
                     /locus_tag="VHT22_10440"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HEX3138019.1"
                     /translation="MKITDCRVIVCSPGRNFVTLKIETDEGVHGVGDATLNGRELAVA
                     SYLTDHVIPTLLGRDPAQIEDLWQYLYRGAYWRRGPVTMSVIAAVDTALWDIKGKVAG
                     LPIYQLLGGRSRDGVMVYGHANGRDVAETLDEVARYIDLGYKAIRAQSGVPGLASTYG
                     VSKDRMYYEPADAALPSENVWSTARYLDHAPRLFEAVRARFGFEHHILHDVHHRLTPI
                     EAARLGKSLEPYRLFWMEDPTPAENQEGFRLIRQHTVTPIAVGEIFNTIYDCQTLITE
                     QLIDFIRTTVVHAGGITHLRRIAQLAELYHVRTGSHGATDLSPVCMGAALNFDLWVHN
                     "
BASE COUNT          374 a          746 c          701 g          378 t
ORIGIN      
        1 acggttattc atgtgactcc tccgttgcgt tgatgacgac ttgagccagg gctggcgtac
       61 tgcgtttgcg agatcctgca aatgctgcct ctgaaaagtt catgggcgcg gcctcggttg
      121 ccggggcttg gctgcggctc gcggccgtgc cggcttgcgc ggcgtcgtga cgcgcctgct
      181 gccgtcgacg ccgcgctgga attcgcgcgc gacgcgcgcc aggtgacgat gcatcgccgc
      241 gcgcgcgcct tcggcgtcgc gggacgcgat cgcgccgacg atcgcatcat ggtcctcgat
      301 ggttttgagc gccagttcgg gggtgtgaaa atgctgctgc atccgccccc agagctcagc
      361 gcgctggtcc cacagtccgg cgacgacgag ctccagcgag ccattgcggg tcgccttcgc
      421 aatcagcaga tgaaactcgc gatcgatcgc ttcgcgcagc gcgaaatccc ggatgccggc
      481 ttccatcgcc cgcacgcttt ggcgcaatgc ctcgagatcc gatggctggc tgctgacggc
      541 agccagcgcc gcaatttctc cttcgaccag cttgcgcgcg tcgagcagct caaacggtcc
      601 ggggccctcg tcggccgccg ccgactggcg cggcacggcg ctcgccgcga cgaaaatgcc
      661 ggtgccgacc cgcacctcga ccaaaccgct gatctccagc gagataatcg cctcgcgcac
      721 cgatgcgcgg ctgacgccga gcgatgcggc gagctcgcgc tcggcaggca gccgcgatcc
      781 cggcagaaac tcgccgctcg cgatcagctg agcgatctgc tcggcgatct gtcggtatag
      841 acgacggggc tcgacggcct gcaacggcaa aagctgctcc tccagtcaac tgcaagccgg
      901 ggcgaatctt tcgttcgcct tcggtggccg ccggatgaat ccggctgcct gtcggcggcc
      961 atcacccggg gatgacgcga cgcttcctcg cgcaagtcgc gaatgacttc gcggcaggac
     1021 gctcgacggc atcttagtcc gcggctatac ttgtggtcaa gtggtcgaac caccagacca
     1081 caagccacgc ggcgcgccag cgctggcccg atcctaaggg caacgcagct cgaagcgcaa
     1141 atggcgaacg cttgccgcgc tggcggccca ctttcgactt ttcgccgatc gcctccgatg
     1201 aagatcaccg actgccgcgt catcgtctgc tcgccaggcc gcaatttcgt cacgctcaaa
     1261 atagaaaccg acgagggcgt acatggtgtc ggcgatgcaa ccttgaacgg ccgcgagctg
     1321 gcggtcgcgt cgtacttgac cgaccacgtg atcccgacgc tgctcggccg cgacccggcg
     1381 caaatcgagg acctctggca atacctttat cgcggcgcct actggcgccg agggccggtg
     1441 acgatgagcg tcatcgccgc cgtcgacacc gccctctggg acatcaaggg caaggtcgcc
     1501 gggctgccga tctatcagct gctgggcggc aggagccgcg acggcgtcat ggtctacggg
     1561 cacgccaatg gcagggatgt cgccgagacg ctcgacgaag tagcgcggta catcgacctc
     1621 gggtacaagg cgatccgcgc gcagtccggc gtgccgggac tggcctccac ctacggcgtg
     1681 tcgaaagacc gtatgtacta cgagccagcc gatgcggcgc tgccgtcgga gaacgtctgg
     1741 tcgaccgcga ggtatctgga ccatgcgccg cggttgttcg aggccgtccg cgcgcgcttc
     1801 ggcttcgaac accatatcct gcacgacgtc catcatcgtt tgacgccgat cgaggcggca
     1861 cgcctgggca aatcgctcga gccttaccgc ctgttctgga tggaggatcc gacgccggcg
     1921 gaaaaccagg agggcttccg cttgatccgg cagcacacgg tgacgccgat cgccgtcggt
     1981 gagatcttca atacgatcta cgactgccag acgctgatca ccgagcagct gatcgatttc
     2041 atccggacca cggtcgtgca tgccggcggc atcacccacc tgcgccgcat cgcgcagctg
     2101 gccgagctct atcatgttcg caccggctcg cacggtgcga ccgacctgtc gccggtatgc
     2161 atgggcgcag cgctgaactt cgacctctgg gtgcacaac
//
LOCUS       DATANS010000701         1651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_497793, whole genome shotgun sequence.
ACCESSION   DATANS010000701 DATANS010000000
VERSION     DATANS010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497793"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VHT22_10445"
     CDS             <1..674
                     /locus_tag="VHT22_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX3138020.1"
                     /translation="ELSAFARQRGILTVVDGAQALGMLEFAARDLGCDFYATCCHKWL
                     GGSHGTGALYVRREMLDRLWPTEPRGLDSVPPVFTPTDADGNTGVPAALHKLGNIVPY
                     SWPALRGVEAALEFQQQVGRSKIEARIRELAIYARLRLQQVTGIELLTPARPGLWAGI
                     LTFRVSGKVATDIAAAVERTHRVHVRAIRWPNSPEGALRLSLHVFSTHDDVEKLIQGL
                     QQALR"
     gene            676..1080
                     /locus_tag="VHT22_10450"
     CDS             676..1080
                     /locus_tag="VHT22_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138021.1"
                     /translation="MDLTYLLLTGGLSGLVSVLLHSILASAYAAVRPGVAVPALQVSD
                     ALLHMLYGTGFALLFWLSWGLAAVVDVPWWVRGLSFGSLCWLSFAVPSVVGVALARGL
                     PVATIAALASRWATTSVIAGLACAWSWERMLR"
     gene            complement(1104..>1651)
                     /locus_tag="VHT22_10455"
     CDS             complement(1104..>1651)
                     /locus_tag="VHT22_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138022.1"
                     /translation="IGDFVDSLRLRAVDVFGYQLGALVTAELAIARPQQVRRVMLWGA
                     PCYSPQERAGLLQAATTPGTREDGGDVVEDWQRMLQRKGANSPPAALAEELGDRLRAG
                     ASGTRALAAAIEYPTLERLPLVKQPALVLRLRDESWDQSPRVRAALPAGSMLDIPDYG
                     QGFLSAAPQRFASIAREFLDR"
BASE COUNT          236 a          554 c          574 g          287 t
ORIGIN      
        1 aagaactgag cgcgttcgcg cgccagcgcg gcattctcac cgtggttgac ggtgcgcagg
       61 ccttgggaat gctggagttc gctgcacgcg acctcggctg cgacttctac gcgacgtgct
      121 gccacaaatg gctcggcggc agtcacggca ccggggcgct gtatgtgcgc cgggagatgc
      181 tcgatcgcct gtggccgacc gagccgcgcg gactcgacag cgtcccgccg gtcttcaccc
      241 cgaccgacgc ggacggcaac accggcgtgc cggcggcctt gcacaagctc ggcaacatcg
      301 tgccttactc ctggcctgcg ctgcgtggtg tcgaagcggc gctggagttc cagcagcagg
      361 tcggtcgcag caagatcgaa gcgcgcatcc gcgagctcgc catctacgcg cgcctgcggc
      421 tgcagcaggt gactggcatc gagctgctga ccccggcgcg cccgggctta tgggccggca
      481 tccttacctt ccgtgtgtcc ggcaaggttg cgaccgacat cgcggcggcg gtggagcgca
      541 cgcatcgcgt gcacgtgcgt gcgattcgct ggccgaacag ccccgagggc gcactacgac
      601 tgtcgctgca cgtcttcagt acgcacgatg acgtagaaaa gctgatccag gggttgcagc
      661 aggcgctgcg ctgacatgga cctcacttat ctactgttga ccggcggcct gagcgggctc
      721 gtgagcgtgc tgctgcattc gattcttgcg tcggcgtacg ccgccgttcg cccgggcgtc
      781 gcagtgccgg cgctgcaggt gtccgacgct ctgctccaca tgctctatgg cacggggttc
      841 gcgctgttgt tctggctgag ctgggggctg gcggcggtcg tcgacgtgcc gtggtgggtc
      901 cgcggcctgt cgttcggcag tctgtgctgg ttgtcgtttg cggtgcccag cgtcgttgga
      961 gttgcgctgg cgcgcggcct gccggtcgcg acgatcgccg cgctcgcttc gcgctgggcg
     1021 accacatcgg tgatcgctgg cctggcgtgc gcgtggagct gggagcggat gctccggtag
     1081 gtcgggcttc ggcccgacgt gatctatcta tccaaaaatt cgcgggcaat cgaagcgaac
     1141 ctttgcggag cggcgctcag gaagccctgg ccgtaatccg gaatgtccag catcgagccg
     1201 gccggcaatg cagcgcgcac gcgcggcgac tgatcccagg attcatcgcg cagccgcaga
     1261 accagcgcgg gctgcttcac gagcggcaga cgctccagtg tcggatattc gatagcggcg
     1321 gccagggcac gcgtaccgct ggcgccggcc cgcagccgat cgccgagctc ctcggccagt
     1381 gcggccggcg gcgagttcgc gcctttgcgc tgaagcatgc gctgccagtc ctcgacgacg
     1441 tcgccgccgt cctcgcgcgt tccgggcgtc gtcgcggcct ggagcagacc cgcccgctcc
     1501 tgcggcgaat aacacggcgc gccccacagc atcacgcgac gcacttgctg cgggcgggcg
     1561 atcgccagct cagcggttac gagcgcgccc agctgatagc cgaacacgtc gaccgcacgc
     1621 agtctcaggc tgtccacgaa atctccgatg g
//
LOCUS       DATANS010000702         3243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_76138, whole genome shotgun sequence.
ACCESSION   DATANS010000702 DATANS010000000
VERSION     DATANS010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76138"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1860
                     /locus_tag="VHT22_10460"
     CDS             <1..1860
                     /locus_tag="VHT22_10460"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX3138023.1"
                     /translation="ADVDVEFVSRFEGGWSGDLSTGAWRGQVVALQGKGQYPLALAQP
                     ADLEAGAQAFHVAGLRGTLAGGRFAVDELRWQDGRLSSRGEFAALPAAPVLAFTGAAA
                     RLSSTLMLAGRWSFAATPRITGSLSVSRESGDLAPVDAPDLALGLTRAELSAEFADDR
                     VHATLIARSRLADADMSADLAPSPASGGRFRGDSPLSLSARVDAKSLRALQGVAGTTA
                     IVDGRLKLDLTGTGTLDRARFTGTVEGDALKVEAAQYGVALKDGRLRARLSDDVVNVS
                     EFSFAAGEGRFAASGTLPASRDIEGARLTWKADKLALFNRPDMRLTLTGAGTLAFQRG
                     GVTVAGALKADEGYFEFRPTGTDAPGDDVIVRGRERPPSRKITQRVPFNVDLDLDFGD
                     SLRFVGQGFDTGLSGKLRVKTTETRDLVANGTIEAVRGTYTTFGQRLTIQRGRLTFDG
                     PLDNPGLDVVAVRKNLQVEAGLEVTGTVRVPHMQLTSNPPVPDNEKLSWLVLGHGLDS
                     ASSADASVLQAGLAALGGPNATPFGQRVAKTIGIDDISVRSSADPTRTGTAGQVVAFS
                     KRLSDKLTMVYEQGLSVANNAIKLEYSLTRTLTVRVEAGVVSGLGLYYSRSYD"
     gene            complement(1929..2005)
                     /locus_tag="VHT22_10465"
     tRNA            complement(1929..2005)
                     /locus_tag="VHT22_10465"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1969..1971),aa:Arg,seq:ccg)
     gene            2206..3168
                     /locus_tag="VHT22_10470"
     CDS             2206..3168
                     /locus_tag="VHT22_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX3138024.1"
                     /translation="MHDEHISPIKTPKQLAIVLVLAFLVPIALFLLLSQLVTGVRRED
                     TGATDAQVLSRIKPVGEVTLAGATGARASMSGEQVFQAVCKTCHEPGIAGAPKVGDKA
                     AWAGPIKKGYETLVQHALNGFQEAGKVMPPRGGNPDLSDIEVESALVYMANRSGANYK
                     EPAATTAAAAAPAAAPPAAAAPAAAPQAVAAPAASGAATAAAAAPAAAGAASAAAAAT
                     PPAAAAAPAAKVGASLDLQSGQAMMQKYGCAACHAIDKPVVGPAYSQVAAKYRGDKSA
                     ATKLEQKVKAGGSGVWGAVPMPPNATVPDADIKALVSWILTLGK"
BASE COUNT          534 a         1093 c         1079 g          537 t
ORIGIN      
        1 gccgatgtcg atgtcgagtt cgtcagccga ttcgaaggtg ggtggtcggg cgatctgtcg
       61 accggcgcgt ggcgcggcca ggtcgtcgca ctgcaaggca aaggacaata tccgctcgcg
      121 ctggcgcagc ccgccgacct cgaagccggc gcgcaagcgt tccacgtcgc cggtctgcgc
      181 ggcaccctcg ccggcggtcg cttcgccgtc gacgagctac gctggcaaga cggacgcttg
      241 tcgagtcgcg gcgaattcgc cgcgttgccg gccgcgccgg tgctcgcttt caccggagca
      301 gcggcccggc tgtcatcgac gctgatgctg gccgggcgct ggtcgttcgc tgcgacgccc
      361 cgcatcaccg gctcgctgtc ggtatcgcgc gagtccggcg atctcgcgcc ggtcgatgcc
      421 ccggacctgg cgctcggcct cacgcgcgcc gagctttccg ccgaattcgc cgacgatcgc
      481 gtgcatgcga cgttgatcgc gcgttcacgc cttgccgatg ccgacatgag cgccgacctc
      541 gctccgtccc ccgcatcggg cggccgcttt cgcggcgact cgccgctcag tctctcagcg
      601 cgcgtcgatg ccaagtcgtt gcgcgcactg cagggggtcg caggtaccac tgcaatcgtc
      661 gacggccggc tgaagctcga ccttaccggc acaggcacac tggaccgtgc gcgtttcacc
      721 ggcaccgtcg aaggcgatgc gctcaaggtc gaagcagccc agtacggcgt tgcgctgaag
      781 gatggtcggc tgcgcgcgcg gctgtcggac gatgtggtca atgtcagcga attctcgttc
      841 gcggcgggcg aagggcggtt cgccgccagc ggcacgctgc cggcgtcgcg tgacatcgaa
      901 ggcgcccgtc tcacatggaa agccgacaag cttgcactgt tcaatcgccc cgatatgcga
      961 ctgacactga ccggcgccgg cacgctggca ttccagcgtg gcggcgtgac cgtcgccggc
     1021 gcgctgaagg ccgacgaagg ctattttgaa ttcagaccca ccggtaccga cgcgcccggc
     1081 gacgacgtga tcgtgcgcgg tcgcgaaagg ccgccctcgc gcaagatcac gcagcgcgtg
     1141 ccgttcaacg tcgacctcga tctcgatttc ggcgatagcc tcaggttcgt cggccaaggt
     1201 tttgatactg gtctaagcgg caagctccgc gtcaagacca ccgaaacgcg cgatctggtc
     1261 gcgaacggga ccatcgaggc cgttcgcggc acctatacga cgttcggcca gcggctgacg
     1321 atccagcgcg gccgcctcac cttcgacggt ccgctcgaca atccggggct cgacgtggtg
     1381 gcagtgcgca agaatctgca ggtcgaggct gggctcgagg ttaccggcac cgtgcgcgtt
     1441 ccgcacatgc aattgacgtc gaatccgccg gttccggaca acgagaagct ctcctggctg
     1501 gtgctgggcc atggactcga tagcgcaagc agcgccgatg cctcggtgct gcaggccggg
     1561 ctcgcggcgc tcggcggtcc gaacgctacg ccgttcgggc agcgcgtcgc gaagacgatc
     1621 ggcatcgacg atataagcgt gcgttcctcc gccgatccca cgcgcaccgg taccgctggc
     1681 caggtcgtcg ccttcagcaa gcgcctgagc gacaagctga cgatggtcta cgaacagggc
     1741 ctttcggtcg cgaacaacgc gatcaagctc gaatattcgc tcacgcgcac gctgaccgtg
     1801 cgcgtcgagg ccggcgtggt cagcggcctc ggcctctatt acagccgatc gtacgattga
     1861 tgctagggga cttttcgccg cgctatgcga ttcggcagcg agtgcctcgc gcgtgcgagc
     1921 gaggatcgtg gcgcgcccga caggagtcga acctgtgacc tttggcttcg gaaaccaaca
     1981 ctctatccaa ctgagctacg ggcgcacgca gccggattct acccgcagcg cagcggagcg
     2041 tccatcgcgc acacgggtgc ggcacacgat cgccggtgga ttttcgcgca gcgtggacgc
     2101 gcgctataat gcgcgcctcg tgctgcggcg gtgcgcgcga acgaagactt cgatgccgct
     2161 gcgtgaggcc ctgtccactc agccaaccct gccgggaatt ccacgatgca cgatgagcac
     2221 atctcaccga taaaaacgcc caagcaactg gcgatcgtgc tggtccttgc attcctggtt
     2281 ccgatcgcgc tttttctgct gctgtcgcaa ctagtcaccg gcgtcagacg cgaggacacc
     2341 ggcgcgaccg acgcgcaggt gctttcgcgg atcaagccgg tcggcgaagt cacgctcgcc
     2401 ggtgcgacag gcgcacgcgc cagcatgagc ggtgagcagg tctttcaggc ggtctgcaag
     2461 acctgccatg agcccggcat cgccggagcc cccaaggtcg gcgacaaggc tgcctgggct
     2521 ggcccgatca aaaaaggcta cgaaactctg gtgcagcacg cgctcaatgg tttccaggag
     2581 gcgggcaagg tgatgccgcc gcggggcggc aatccggacc tttccgacat cgaagtcgag
     2641 agcgcgctcg tttacatggc caatcgttcc ggtgcgaatt acaaggagcc ggccgcgacg
     2701 acggccgctg ccgctgcacc tgctgctgcg ccaccggctg ccgctgcacc tgctgccgcg
     2761 ccacaggctg ttgctgcgcc cgcggcttcc ggcgcggcaa ctgctgccgc cgctgctccc
     2821 gcggccgccg gtgcggcaag tgctgccgcc gccgcgactc cgccggccgc tgctgccgcg
     2881 cccgcagcaa aggttggtgc gtcgctcgat ctgcagtccg gtcaggcgat gatgcagaag
     2941 tacgggtgcg ccgcgtgcca cgcgatcgac aaaccggtcg tcggcccggc gtacagccag
     3001 gtcgcggcga aatatcgcgg cgacaaaagc gcggcgacca agctcgagca gaaggtcaag
     3061 gcaggcggct cgggcgtgtg gggagccgtg ccgatgccgc cgaacgccac tgtgcccgat
     3121 gccgacatca aggcgctggt cagctggatc ctgacgctcg ggaagtagcc aagcacgcgc
     3181 ggccagcgat tgcgctggtg gttagcctgc ggtttcaaat gcagtcacgt ttgtccgacg
     3241 atg
//
LOCUS       DATANS010000703         1766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_620315, whole genome shotgun sequence.
ACCESSION   DATANS010000703 DATANS010000000
VERSION     DATANS010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620315"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VHT22_10475"
     CDS             <1..423
                     /locus_tag="VHT22_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HEX3138025.1"
                     /translation="RLVDKIVARLGDDLAGKTFALWGLAFKPDTDDMREAPSRAIIAG
                     LLERGASVAAYDPVAIDEARRVFGQTAGLSFASSPMAAVAGADALLVVTEWKEFRSPE
                     FAEIRRQLKQPLLFDGRNLFDPALVRAAGIEYFGIGRS"
     gene            454..1401
                     /gene="rfaE1"
                     /locus_tag="VHT22_10480"
     CDS             454..1401
                     /gene="rfaE1"
                     /locus_tag="VHT22_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEX3138026.1"
                     /translation="MPTSARRFSDFRDAVRRARVLVVGDAMLDRYWFGDVDRISPEAP
                     VPVVKIARSEERPGGAANVARNAAALGARVTLISVIGDDEPGHVLERLLRGESVHTSL
                     HRDPSLPTTVKLRVIGRQQQLLRIDFETVPSPLALAAKLADYERTLAAFDVVVLSDYG
                     KGGLSQIERMIALAKAAGKPVLVDPKGEAYLRYRGATMLTPNRAEFRQVVGPWRNEAE
                     LAEKAQILRSELALDALLITRSEEGMSLYTDAGAQHIPAQGREVFDVSGAGDTVIATV
                     GTLLGAHADIGSAVRIANKAAGIVVGKLGTAVVHPDELG"
     gene            1429..>1766
                     /locus_tag="VHT22_10485"
     CDS             1429..>1766
                     /locus_tag="VHT22_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX3138027.1"
                     /translation="MAKTKTAYVCTECGGQSSKWQGQCPNCGVWNTLVETIAATPSSR
                     FQALAGAPSAVRTLSSVETQDNARTPTGIEEFDRVLGGGLVSGGVVLLGGDPGIGKST
                     LLLQAMAALGG"
BASE COUNT          277 a          566 c          633 g          290 t
ORIGIN      
        1 cgcctggtcg acaagatcgt cgcgcggctc ggcgacgatc ttgccggcaa gacgttcgcg
       61 ctatggggac tcgcattcaa gcccgatacc gacgacatgc gcgaagcgcc gtcgcgcgcg
      121 atcatcgctg gattgctcga gcgtggcgcg tccgttgcgg cttacgatcc ggtcgcgatc
      181 gacgaggcga ggcgcgtctt cggccagacc gcgggcctgt ctttcgcgag ctcgccgatg
      241 gccgccgtcg ccggggccga cgcgctgctg gtcgtcaccg agtggaagga attccgcagt
      301 cccgagttcg cggaaatccg gcgccagctg aagcagcctc tgctgttcga cggccgcaac
      361 ctgttcgacc cggcgctggt gcgtgcggca ggcatcgaat atttcgggat cggtcgcagc
      421 tagcgccgtt tcgttcaacg tgagcaagcc ctgatgccta cgagcgcgcg gcgcttcagc
      481 gattttcgcg atgcggtccg tcgcgcccgc gtgctggttg tcggcgacgc gatgctcgat
      541 cgttactggt tcggtgacgt cgatcgcatt tcgcccgagg cgccggtgcc ggtcgtgaag
      601 atcgcgcgca gcgaggagcg tccggggggg gccgccaacg tcgcgcgcaa cgccgcggcg
      661 ctgggcgcgc gtgtgacgct gatcagcgtc atcggcgacg acgagccggg gcatgtgctc
      721 gagaggcttc ttcggggcga aagcgtgcat acgtctctgc accgcgaccc ctcgctgccg
      781 acgacggtga agctccgtgt catcggacgc cagcagcagc ttttgcgcat cgacttcgag
      841 accgtgccgt cgccattggc gctggcggcg aagctcgcgg actatgagcg cacgctggcc
      901 gctttcgatg tggtcgtgct ctccgactac ggcaagggcg gactgtcgca gatcgagcgc
      961 atgattgccc tcgccaaggc ggccggcaaa ccggtgttgg tcgacccgaa aggcgaggcc
     1021 tacttgcggt accgcggcgc gacgatgctg acgccgaacc gtgccgaatt ccgccaggtc
     1081 gtcggaccgt ggcgcaacga agccgaactc gcggaaaagg cgcagatcct gcgcagcgaa
     1141 ctcgcgctgg atgcgctgtt gatcacgcgc tcggaagagg gaatgtcgct ctacaccgat
     1201 gccggagcgc agcacattcc ggcgcagggg cgcgaagtct tcgacgtctc cggggccggc
     1261 gatacggtga tcgcgaccgt gggcaccctg ctaggcgcgc acgccgatat cggcagcgca
     1321 gtgcgcatcg ccaacaaggc ggccgggatc gtggtcggca agctgggcac cgctgtcgtg
     1381 cacccggacg agctcggctg acatgcggca acggtgcgac ggggcctgat ggccaagacg
     1441 aagaccgcgt acgtatgcac cgagtgcggc ggtcaaagca gcaagtggca gggtcaatgc
     1501 ccgaattgcg gcgtctggaa cacgctggtg gagacgatcg ccgcgacgcc ctcgtcgcgc
     1561 ttccaggcgc tggccggcgc gccgagtgct gtgcggacgc tgtccagcgt cgaaacccag
     1621 gacaacgcgc gcacgcccac ggggatcgag gaattcgatc gcgttctggg cggcgggctg
     1681 gtgtccggcg gcgtggtgct ccttggcggt gaccccggca tcggcaagtc gaccttgctg
     1741 ttgcaggcga tggccgccct cggcgg
//
LOCUS       DATANS010000704         2545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_118992, whole genome shotgun sequence.
ACCESSION   DATANS010000704 DATANS010000000
VERSION     DATANS010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118992"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..889
                     /gene="trmB"
                     /locus_tag="VHT22_10490"
     CDS             203..889
                     /gene="trmB"
                     /locus_tag="VHT22_10490"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEX3138028.1"
                     /translation="MNASRGQPRRPIRSFVLRQGRFSPAQQRAREALLPRFGVPYAPT
                     LLDLAALFGRDNPKIVEIGSGMGEATAAIAEAQPDRDFLAIEVHSPGVGGLLKLIDEK
                     DLANVRIVQHDAAEVVADMIAPGALAGIHVFFPDPWPKKRHHKRRLLQADFIHALAQR
                     LGPGGYLHVATDWEDYATDVLAAASAEPLLVNTTALFAPRPAYRPATKFEQRGLKLGH
                     GVWDIVFERR"
     gene            complement(950..>2545)
                     /locus_tag="VHT22_10495"
     CDS             complement(950..>2545)
                     /locus_tag="VHT22_10495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3138029.1"
                     /translation="PDAGLVGAKLVYPDGRLQEAGGIVWRDGSAWNYGRDDDPARPEY
                     NYLRPVDYCSGACLAVPRRLFGELGGFDVRYAPAYYEDTDLAFAVRAAGRRVYYQPAA
                     TIVHFEGATSGTDESAGIKRHQTINRATFAAKWSEALARHGANGVAPEREHDRWATRR
                     ALVIDACMLTPDQDSGSLRMQQVMDIVTGLGCKVTFVADNLEYRKPYVQALQQQGVEV
                     QFHPYASSLAMLLGERGGEFDLVLLSRHYVAARHMATVRAFAPRALIAFDTVDLHFLR
                     EERLADLDASRAARGAAAAKRAEELALIGRADVTLVVSEVERDLLADLAPQAKVIVLS
                     NIHDRHEHGRPFAQRAGLLFVGGFRHPPNTDAVLWYAKEILPRIRTGLPGVKSYVVGA
                     DPPATIRSLATHDLVIAGFVPDLTPYLEGCRVSISPLRYGAGVKGKVNQAMSFGLPVV
                     ATTPSIEGMHLTPGIDVLVGDDPQAFADAVVFAYQDQALWERLAAGGRDNVSRHFSRE
                     VAAAAIAELLMMADRKTLDRRSA"
BASE COUNT          433 a          879 c          821 g          412 t
ORIGIN      
        1 agctgcgctg gacgcccgcc gcggcatggg ccgatgccgg cgtgacctgg cgcgacgccg
       61 acacgctggc aatcgaattc aacgtcgacc cggctagcgc tccatcgacg atcgcgcgca
      121 agctgaccga tccgacctgg acgcggatcg caccgtgagc ccgcagtgag cacgccgtga
      181 gcgtcaaaac agtcttgcgc cgatgaacgc atcgcgcggg caaccgcgcc ggccgatccg
      241 cagttttgtg ctgcgacagg gccggttttc cccggcacag cagcgcgccc gcgaggcgct
      301 gctgccgcgt ttcggcgttc cgtacgctcc gacgctactc gacctggctg cgctcttcgg
      361 ccgcgacaat ccgaagatcg tcgagatcgg gtccggcatg ggggaagcga ccgccgcgat
      421 cgccgaagcg cagcccgatc gggatttcct ggcgatcgaa gtgcattctc ccggcgtcgg
      481 cgggctgctg aaactgattg acgaaaagga cctggccaac gtccgcatcg tgcagcacga
      541 tgcggccgaa gtcgtcgccg atatgatcgc gccgggcgcc ctcgccggaa tccatgtttt
      601 tttccccgac ccctggccca agaagcggca ccacaagcgg cggctgctgc aggccgactt
      661 catccatgcg ctcgcacagc gcttgggccc gggcggctat ctgcatgtgg cgaccgactg
      721 ggaagactac gcgaccgatg ttctagcggc cgcgagcgcc gaaccgctgc tggtcaatac
      781 gacggctttg ttcgctccgc gtcccgccta tcggccggca accaagttcg agcagcgtgg
      841 attgaagctc ggccacggcg tctgggacat cgtgttcgag cgccgctgaa attgcggcgt
      901 acgccactgg ccgccgtggg gtacgcgcgt caacgttcga tgcgccgccc tatgccgatc
      961 gccggtcgag cgtcttcctg tccgccatca tgagaagctc ggcgatcgcc gccgccgcga
     1021 cctcgcggga aaagtggcgg ctcacgttgt cgcgaccgcc cgcagcaagc cgttcccaca
     1081 gtgcctgatc ctgataggcg aagaccaccg catcggcaaa cgcctgcgga tcgtcgccga
     1141 ccaggacatc gatccccggc gtcaggtgca tgccttcgat tgatggcgtc gtcgccacca
     1201 ccggcagacc gaaactcatc gcctggttga cctttccctt gacgccggcg ccgtagcgca
     1261 atggcgagat cgagactcgg cagccctcga ggtatggcgt cagatccgga acgaagccgg
     1321 cgatcaccag gtcgtgggtc gccaagctgc ggatcgtcgc cggcggatcg gcgccgacga
     1381 cgtagctctt aacaccgggc agcccggttc gaattctcgg caggatctcc ttcgcgtacc
     1441 agaggacggc atcggtattc ggcggatgcc ggaagccgcc gacgaacagc aaaccggcgc
     1501 gctgcgcaaa cggacgaccg tgctcgtgcc ggtcgtggat gttcgacagc acgatcactt
     1561 tcgcttgcgg cgcgagatcg gccagcaggt cgcgctcgac ttcgcttacg accagcgtga
     1621 cgtccgcccg gccgatcaat gccagctcct cggcccgctt tgccgcggcc gctccccgcg
     1681 ccgcgcggct ggcatcgaga tccgcgagtc tttcttcgcg caaaaaatgc aggtcgacgg
     1741 tatcgaatgc gatcagcgcc cgcggtgcga aggcccgcac cgtcgccatg tgccgtgccg
     1801 cgacatagtg ccgcgacagc aacacgagat cgaattcgcc gccgcgctcg ccgagcaaca
     1861 tcgccagcga gctggcgtac ggatggaact gcacctcgac gccctgttgc tgcaaggcct
     1921 gaacgtaggg tttccggtat tcgagattgt cggcgacgaa cgtcaccttg cagcccaggc
     1981 cggtaactat gtccatcacc tgctgcatcc gcagcgaccc cgaatcctga tcgggcgtca
     2041 gcatgcaagc gtcgatcacc agcgcgcggc gggtggccca tcggtcgtgc tcacgctccg
     2101 gagcgacgcc gttcgcgccg tggcgggcca gcgcttccga ccatttggct gcgaacgtgg
     2161 cgcgattgat cgtctggtga cgcttgatac cggccgactc gtcggtaccc gaggtcgcac
     2221 cctcgaaatg gacgatcgtc gccgccggct ggtaatagac acggcggccc gcggcgcgca
     2281 cggcgaacgc gagatcggtg tcctcgtaat acgccggtgc gtagcggaca tcgaagccgc
     2341 cgagctcgcc gaacaggcgg cgcggcaccg cgaggcaggc ccccgagcag taatcgaccg
     2401 ggcgcagata gttgtattcg ggccgggccg ggtcgtcgtc gcgaccgtag ttccaggcgg
     2461 agccgtcgcg ccagacgatg ccgcctgctt cctgcagcct gccatccgga tacacgagct
     2521 tcgcgccgac caggcccgca tccgg
//
LOCUS       DATANS010000705         1853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_243078, whole genome shotgun sequence.
ACCESSION   DATANS010000705 DATANS010000000
VERSION     DATANS010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243078"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VHT22_10500"
     CDS             <1..327
                     /locus_tag="VHT22_10500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3138030.1"
                     /translation="KNQGIQFPLARAYVNVEAANLMRFKAAALFDGGKQCGAEANMAK
                     LLAADASWEAGNVCLQTHGGFGFAAEYDVERKFRETRLYQVAPISTNLILSYIGEHVL
                     GMPRSF"
     gene            337..1518
                     /locus_tag="VHT22_10505"
     CDS             337..1518
                     /locus_tag="VHT22_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX3138031.1"
                     /translation="MTRPLDGITIVALEQAVAAPFATRQLADLGARVIKIERPGVGDF
                     ARDYDQTVKGQSAYFVWLNRSKESLTLDVKHARAAEILERLIGNADVFIQNLAPGAAG
                     RLGLDAATLLANHPRLIVCDVSGYGDSGPYAHKKAYDLLVQSEAGVLSVTGTPETQTK
                     VGISIVDISAGMYAYSGILTALYQREKTGKGTRVDVTMFESLAEWMSHPLNYTHFGGK
                     PPQRSGPDHATIVPYGRFKCGDGAYVMLGIQNEREWAKFCAGVLGMPGLATDDKYSNN
                     TKRTSRRAEVVALIEEVFSRMKAEEVVARLDAADIANARINTPQEVWDHPQLKARDRW
                     REVGSPAGPLPVLLPPVTMPEFEARLDPIPAIGEHTDRILADIGYSAADIAELRKSAA
                     V"
     gene            1539..>1853
                     /locus_tag="VHT22_10510"
     CDS             1539..>1853
                     /locus_tag="VHT22_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX3138032.1"
                     /translation="MDTVTSTDTTSSTRTLCEFLSAIRFEQIPAPVIARTEELFLDWF
                     ASALAGKDARPTRVMEKFAETMGPRDGDSEILVSRKKTSPIFAALINGAASHFVEQDD
                     LHN"
BASE COUNT          371 a          574 c          579 g          329 t
ORIGIN      
        1 aagaaccagg gcatccagtt tccgctcgcc cgcgcttatg tgaacgtcga ggcggcgaac
       61 ctgatgcgct tcaaggctgc ggcattgttc gatggcggca agcagtgcgg cgccgaagcg
      121 aatatggcca agctgctcgc ggcagacgcg tcgtgggaag caggcaacgt ctgcctgcag
      181 actcacggcg gattcggctt tgccgccgaa tacgacgtcg agcgcaagtt ccgtgaaacc
      241 aggctctatc aggttgcgcc gatttcgacc aacctcatcc tatcgtatat cggcgagcat
      301 gtattgggaa tgccgcgctc gttctaggct gccgctgtga ccagaccact tgacgggata
      361 accatagtcg cgctggagca ggccgtcgcg gcgccattcg cgacgcgcca gcttgccgat
      421 ctcggcgcgc gcgtgatcaa gatcgagcgc cccggcgtcg gcgattttgc acgcgattac
      481 gatcagacgg tcaaaggtca atccgcgtat ttcgtctggc taaaccgctc caaagaaagc
      541 ctgacgcttg acgtgaagca cgcgcgcgcc gcagagattc tcgagcgcct gatcggaaac
      601 gccgacgtgt tcatccagaa tctggcaccc ggcgctgccg gccggctggg tctcgatgca
      661 gcgacgctgc tcgcgaatca tccgcgcctg atcgtgtgcg atgtttccgg ctacggcgac
      721 agcgggccgt acgcgcacaa gaaagcgtac gatctgctgg tccagagcga agcgggtgtt
      781 ttgtcagtca cgggcacgcc agagacccag accaaggtcg gcatctccat cgtcgacatc
      841 agcgcgggca tgtatgcgta ttccggtatt ctcactgcgc tctatcagcg cgagaagacc
      901 ggcaagggca cgcgcgtcga cgtcacgatg ttcgagtcgc tcgccgagtg gatgtcgcat
      961 ccgttgaatt acacgcactt cggcggcaag ccgccgcagc gcagcgggcc tgaccatgcc
     1021 acgatcgttc cgtacggccg cttcaagtgc ggcgacggcg cctacgtgat gctcggcatc
     1081 cagaacgagc gcgagtgggc gaagttctgc gccggcgtcc tcggcatgcc cggactcgcg
     1141 actgacgaca agtacagcaa caatacgaag cgcacttcgc gtcgagccga agtcgtcgcg
     1201 ctgatcgaag aggttttctc tcgcatgaaa gcggaagaag tggttgccag gctcgatgct
     1261 gccgacattg cgaatgcgcg catcaatacg ccgcaggaag tgtgggacca tccgcaactg
     1321 aaggcgcgcg accgctggcg cgaagtcggc tcgccggcgg gtccgttgcc ggtgctgctg
     1381 ccgccggtta cgatgccgga attcgaggcg cggttagacc cgataccggc aatcggcgag
     1441 cataccgacc gcatcctcgc cgatatcggc tacagcgccg ccgacattgc ggaattgcgc
     1501 aagagcgcgg ccgtctgaat agatcaagga gcattgccat ggataccgta accagcaccg
     1561 acacgacgtc gtccacccgc acgctatgcg aatttctgtc ggcgatccgg ttcgagcaga
     1621 tccccgcgcc ggtaatcgcg cgcaccgaag aactgtttct cgactggttt gcgtcggcgc
     1681 tcgcgggcaa ggacgcgcgg cccacccgcg tcatggaaaa attcgcggag acgatgggac
     1741 cgcgtgacgg cgacagcgag atcctggtct cacgtaaaaa gacatctcca atcttcgcgg
     1801 cgctgatcaa cggcgcggcg tcgcatttcg tcgagcagga cgatttgcac aac
//
LOCUS       DATANS010000706         2913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_34531, whole genome shotgun sequence.
ACCESSION   DATANS010000706 DATANS010000000
VERSION     DATANS010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34531"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VHT22_10515"
     CDS             complement(<1..1001)
                     /locus_tag="VHT22_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX3138033.1"
                     /translation="MAMRIEDYAMIGNNRTAALVGRNGSIDWLCAPRFDAPACFAALV
                     GTSDHGRWLLAPRGTVRRVQRRYRDQTLVLETDFHTAAGAVRIVDCMPPWEERTDVVR
                     IVEGLRGSVRMHMELVIRGGYGVIVPWVRRVDGALLATAGPESLELRCAVPLHGKGFT
                     TVASFTVARGQRVAFVLTHFASHLPRPLPIDADAAVDATARSWRAWSDQSTYDGRWRE
                     PVMRSLITLKALTYAPTGGIAAAVTTSLPEAIGSVRNWDYRYCWPRDATSTLYAFLLA
                     GYREEAQAWRRWLLRAAAGRPEDMQTVYGLSGERQLTEYTVPWLPGYEGSAPVRVGNA
                     "
     gene            1213..1623
                     /locus_tag="VHT22_10520"
     CDS             1213..1623
                     /locus_tag="VHT22_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138034.1"
                     /translation="MQATAAIPAIPATAASTRIWYRLALLYFVTGTGLGVYMGVSQDH
                     TMFPVHAHLNLLGWASLALTGFIYDRFPAATRTRLYPVHFWVYNLTLPTAMLALALLF
                     KGNEAFGPVAGIASIVLFASIVTFAVNVWRNSDS"
     gene            1633..2379
                     /locus_tag="VHT22_10525"
     CDS             1633..2379
                     /locus_tag="VHT22_10525"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX3138035.1"
                     /translation="MSRPAADGPPTLEVAGAVATIRLRRPAEHNRIDPDDVAVIRGYL
                     DNGKLYPAARSLVFTGTGDTTFSSGYTVDAIVGRLDRNFEQLLDAIERFPLPTICALN
                     GSVYGGATDLALCCDFRLGVRGSRMFMPAARFGLHYYPGGLRRAVAALGAAQAKRIFL
                     TAQTIDADEMLRIGFLTELLDRRDLPGRIDAYVAALAECEPGVVQSMKRQIDAIAGGD
                     RANAESRSAYEASLGSDELRRRLARTGRQG"
     gene            2635..>2913
                     /locus_tag="VHT22_10530"
     CDS             2635..>2913
                     /locus_tag="VHT22_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138036.1"
                     /translation="MDDSSDAVANFPPEQRAIRDSCFHPAGTFIEFKQAEIDQSVCNR
                     FEQQVAKYPGRIAVRTTRHTLTYDELNRASNRIARAILDRSAAVQQPIA"
BASE COUNT          502 a          990 c          924 g          497 t
ORIGIN      
        1 gcgttgccga cgcgcaccgg tgcggagccc tcgtaaccgg gcaaccatgg caccgtgtat
       61 tcggttagct ggcgttcgcc tgacaatcca tacaccgtct gcatgtcctc aggcctgccg
      121 gcggcggcac gcaacagcca acgtcgccag gcctgagcct cttcgcggta gccagcgagc
      181 aggaacgcgt agagcgtaga ggtcgcatcg cgtggccagc aataacggta gtcccagttg
      241 cgcacgctgc cgatggcttc cggcagcgaa gtcgtcaccg cggcggcgat gccgccggtc
      301 ggcgcgtaag tcagcgcttt cagggtgatc agcgagcgca tgaccggctc gcgccagcgc
      361 ccatcgtaag tggattggtc gctccaggct cgccaactcc gcgcggtcgc gtcgaccgcc
      421 gcgtcagcgt cgattggcag cgggcgaggc agatgcgagg cgaaatgagt caggacgaag
      481 gcaacacgct ggccgcgcgc gacggtaaac gaggcaaccg tggtgaatcc cttgccgtgc
      541 agcggcacag cgcagcgcaa ttccagcgat tcggggccgg cggtcgcaag cagagccccg
      601 tcgacgcgcc ggacccaagg cacaatgacg ccgtagccgc cgcgaatgac cagttccatg
      661 tgcatccgaa cgcttccgcg cagaccctcg acgatgcgca cgacgtcggt gcgctcctcc
      721 caaggcggca tgcagtcgac gatgcgcaca gcgcctgcgg cggtgtgaaa gtcggtctcg
      781 agcaccagcg tctggtcgcg gtaacggcgc tgcacccggc gcacagtgcc gcgcggcgcg
      841 agcagccacc gcccgtgatc ggacgtgccg accagcgccg cgaagcaggc cggcgcatcg
      901 aagcgcggag cgcaaagcca gtcgatcgaa ccgttgcgcc cgaccagcgc cgcggtgcgg
      961 ttgttgccga tcattgcgta gtcttcgatc cgcatcgcca tcctggtcta tccgtgttct
     1021 tcttaggatg cgcggacgcc gcctaccgtt ccgccggcgg accgtccgca gcacgactct
     1081 cccgcggcgg ccggcggcgc cttgcgactt cgttggccgc gccggtcgca cgcgctatag
     1141 ttcggcgcca gccctcaccc gcgagcaggg ccgggaggag ctcgacatcg cgatcatcca
     1201 cgaggaggcc acatgcaagc aaccgctgcc atacccgcga tcccagcgac agccgcgtcg
     1261 acgcgaatct ggtaccgcct ggcgttactg tattttgtca ccggcacggg attgggtgtc
     1321 tatatgggcg tgtcgcagga ccacacgatg tttccggtgc acgcgcatct gaaccttctc
     1381 ggctgggcct cgctggcgct taccggattc atctacgacc ggttccctgc cgcaacacgg
     1441 acgcgccttt atccggtgca cttctgggta tataacctca cgctgccgac ggcgatgctc
     1501 gcgttggcat tgctgttcaa gggtaacgaa gcgttcgggc cggtcgcggg aatcgcctcg
     1561 atcgtcctgt ttgcatcgat cgtgaccttt gcagtcaatg tctggcgcaa cagcgactcg
     1621 tgatcggcgg tgatgagtcg tcccgctgcc gatggtccgc ccacgcttga agtcgccggc
     1681 gcggtcgcca cgatccggtt gcgccggcct gccgagcaca accgcatcga tccggacgat
     1741 gtcgcggtca ttcgcggcta cctggacaac ggcaagctct atccggcggc gcggtcgctg
     1801 gtattcaccg gcaccggaga cacgacgttc agctcgggat acaccgtcga tgcgatcgtc
     1861 ggccgtctcg accgcaattt cgagcagctg ctcgatgcca tcgagcggtt tccgctgccg
     1921 acgatatgcg cgctgaacgg cagcgtctac ggaggagcca ccgaccttgc actgtgctgc
     1981 gatttccggc tcggcgtgcg cggctcgcgc atgttcatgc ccgccgccag attcgggttg
     2041 cactactatc cgggagggct ccggcgcgcg gtggcggcgc tgggtgcggc gcaggccaag
     2101 cggatattcc tgacagcgca gacgatcgac gccgacgaga tgctgcgcat cggattcctg
     2161 accgagctgc tcgaccgccg cgatcttccc gggcggatcg acgcctatgt cgctgcgctg
     2221 gccgaatgcg agccgggcgt cgtccagtcg atgaagcggc agatcgacgc gatcgcgggc
     2281 ggcgatcgcg cgaatgccga atctcgctcc gcatacgaag cctcgctcgg atcggacgaa
     2341 ttgcgccggc gcctggcgcg gaccgggcgt caaggctaga ggttcatcgc attcctacgc
     2401 agatgtcggc gcacgcctac agcagaatcg gcgatccgcc gatatccgcc ggccgatgct
     2461 cgagcataat gcggtcaggc agggcagatc caaacctacc ggtcgattgc ctcaggttga
     2521 ccgggcgatc tgatccctgc cctttccatt tgtccttcgc cttcgacgac ccgccgcccc
     2581 gcgcgcgccg tatacagccc gcttgccact cgatccacgg gcaacggcga tagaatggat
     2641 gactcctccg acgctgtcgc gaacttcccg ccagagcaac gcgccatccg ggacagctgc
     2701 ttccatccgg cgggcacatt catcgagttc aagcaggcag agatcgacca atccgtctgc
     2761 aatcgtttcg aacagcaggt cgccaaatat cctggccgaa tcgccgtcag gaccacaagg
     2821 catacgctca cttacgacga gctgaatcga gcctccaacc ggatcgcgcg tgccatcctg
     2881 gatcggtctg ccgccgtgca gcagcctatc gcg
//
LOCUS       DATANS010000707         2480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_830565, whole genome shotgun sequence.
ACCESSION   DATANS010000707 DATANS010000000
VERSION     DATANS010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830565"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VHT22_10535"
     CDS             complement(<1..383)
                     /locus_tag="VHT22_10535"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3138037.1"
                     /translation="MNLEAQPASGRVPEPGVYVGAGGQLKAAREAAGLSLDQVAQQLK
                     LAPRQVRALEEENFGMLPGRTFTRGFMRNYARLVNLDPDLLVAHLPDAAHAPSLEPPP
                     LHSTGTTMAELPTAYAHTPSFGRWL"
     gene            complement(383..1186)
                     /gene="pilW"
                     /locus_tag="VHT22_10540"
     CDS             complement(383..1186)
                     /gene="pilW"
                     /locus_tag="VHT22_10540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEX3138038.1"
                     /translation="MPYRLLTIAAVSLLAACANSPFAPNPTTTPPERPTREAPPPQQM
                     ASAAERARLHTDLGSGYYERGQMDVALGELDTAVKLDPNYAPAYNIYGLIYAVLGEDR
                     KAEQNFERALALAPSNPDIHHNWGWYLCQHKRERESLAQFESAVADPLYRTPEIALVN
                     AGRCAQAIGDVRAAEGYFRRALSGQPNNVLASYGLAQIAYKEGRYDEARAAMRRVSQA
                     NNAPPEALRLGMCVERKLGDRQSEMSYTSQLRNRYPDAPETRAITEACE"
     gene            complement(1200..2315)
                     /gene="rlmN"
                     /locus_tag="VHT22_10545"
     CDS             complement(1200..2315)
                     /gene="rlmN"
                     /locus_tag="VHT22_10545"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX3138039.1"
                     /translation="MSQMINLLDLDSAAMTAFFAERGEKPFRAKQALRWVHHRLADRV
                     DAMSDLAKATREALARDAEIRAPSVIRDSTASDGTRKWLLDVGNANAIEAVYIPETDR
                     GTLCISSQAGCALDCAFCSTGKQGFNRNLSVGEIIGQLWHANRALLADGIAAPWVEHG
                     RHPITNVVMMGMGEPLANFDNVVAALQIMLDDNAYGLSRRRVTLSTSGLVPALDRLRD
                     ACPVALAVSLHAPNDALRDRLVPINRKYPLAELLAACLRYIEKAPRDFVTFEYVMLDH
                     VNDGAEHARELVSLVRDVPCKFNLIPFNPFPNTEFTTSSRTRILAFQRILRDAGLVAT
                     IRKTRGEDIDAACGQLAGQVQDRTRRTLRRIQPVAAQ"
     gene            complement(2315..>2480)
                     /locus_tag="VHT22_10550"
     CDS             complement(2315..>2480)
                     /locus_tag="VHT22_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX3138040.1"
                     /translation="ELMGATDPKKAAAGTIRADFAQSIDANAVHGSDAPETASVEIAY
                     FFPAMSIYQR"
BASE COUNT          395 a          790 c          858 g          437 t
ORIGIN      
        1 atcagccagc ggccgaagct cggtgtatgg gcatacgcgg tcggcaactc agccatcgtc
       61 gtaccggtcg aatgcagcgg cggcggctcg agcgacgggg cgtgcgccgc atcgggaagg
      121 tgcgcgacta gcaggtcggg atcgaggttc acaaggcggg cgtagttgcg catgaagccg
      181 cgcgtgaacg tcctgcccgg cagcatgccg aagttttctt cctcgagcgc cctgacctgg
      241 cgcggcgcca gcttgagctg ctgagccacc tggtcgagcg aaaggccggc ggcctcgcgc
      301 gcggccttga gctgccctcc ggcgccgacg tacacacccg gctcgggcac gcggccgcta
      361 gccggttgcg cttcgagatt catcactcgc aggcctcggt gatcgcccgc gtttccggcg
      421 cgtccggata gcggttgcgc aactgcgacg tgtacgacat ctcggattgg cggtcgccga
      481 gcttgcgctc gacgcacatg ccgagccgca gggcttccgg cggcgcattg ttcgcttgac
      541 tcactcgccg catcgccgcg cgcgcctcgt cgtagcggcc ttccttgtag gcgatctggg
      601 caagtccgta gcttgccagc acgttgttgg gctgtccgga gagcgcacgc cggaaatacc
      661 cttcggcggc gcgcacgtcg ccgatcgcct gcgcgcatcg gccggcgttg accagcgcga
      721 tctccggcgt ccggtacaac ggatcggcga cggcgctttc gaactgagcc agcgactcgc
      781 gctcgcgctt gtgctgacaa aggtaccagc cccagttgtg atggatatcg ggattgctcg
      841 gcgcgagcgc cagcgcgcgc tcaaagttct gctcggcctt gcggtcctcg cccaggaccg
      901 cgtagatcag tccgtagatg ttgtacgccg gcgcgtaatt cgggtcgagc ttgaccgcgg
      961 tgtcgagctc gccgagcgcg acatccattt ggccgcgctc gtagtagccg ctgccgaggt
     1021 cggtatgcag cctcgcccgc tcggccgcgc tcgccatctg ctgcggcggg ggcgcctcgc
     1081 gtgtcggccg ctcgggcggc gtcgtcgtag gattgggagc gaacggactg ttggcgcacg
     1141 cggcgagcag cgacacagcg gcgatggtca gaagacggta aggcatggcg ggcaagcctc
     1201 tattgagccg caaccggctg gatccgacgc agcgtgcggc gcgtccggtc ctgcacctgg
     1261 ccggcaagct gcccgcaggc agcgtcgata tcctcgccgc gcgtcttgcg tatggtcgcg
     1321 accaggccgg cgtcgcgcag gatccgctgg aatgccagga tccgcgtgcg cgagctggtc
     1381 gtgaattcgg tgttcggaaa gggattgaat ggaataaggt tgaacttgca cggcacgtcg
     1441 cgcaccagag acaccagctc gcgcgcgtgc tcggcgccgt cattgacgtg gtcgagcatc
     1501 acatattcga aggtcacgaa atcgcgcggc gccttttcga tgtagcgcag gcatgccgcc
     1561 agcagctcgg ccagcggata tttgcgattg atgggaacga gccggtcgcg cagcgcatcg
     1621 ttgggcgcgt gcaggctcac cgccagcgcc accgggcaag cgtcgcgcaa gcggtcgagc
     1681 gcgggcacca ggcccgaagt cgacaaggtc acgcggcggc gcgagagtcc ataggcattg
     1741 tcgtcgagca tgatctgcag cgccgcgacg acgttgtcga aattggccag cggctcgccc
     1801 atacccatca tcaccacgtt ggtgatcgga tggcgtccat gctcgaccca gggcgcggcg
     1861 atgccgtcgg cgagcagcgc gcgattggca tgccaaagct gcccgatgat ctcgccgacg
     1921 ctcaagttgc ggttgaagcc ctgctttccg gtcgagcaga atgcgcagtc gagcgcacaa
     1981 ccggcctgag aggagatgca cagcgtgccg cgatcggttt cgggaatgta aacggcctcg
     2041 attgcgttcg cgttgccgac gtcgagcagc catttgcgcg tgccgtcgga cgcagtcgag
     2101 tcgcgaatga ccgacggggc gcggatctcg gcatcgcgcg cgagcgcctc gcgcgtcgcc
     2161 ttggccaggt cgctcatcgc gtcgactctg tcggcgagcc ggtggtggac ccagcgcagc
     2221 gcctgcttgg cgcgaaacgg cttttctccg cgctcggcga aaaatgccgt catcgcggcg
     2281 gagtcgaggt cgagcagatt gatcatttgc gacatcagcg ctggtagatg ctcatcgcgg
     2341 gaaagaaata ggcgatttcg acgctggctg tttcgggcgc gtccgagccg tggaccgcat
     2401 tggcatcgat tgattgagcg aaatcggcgc ggatcgttcc cgctgcggcc ttcttcggat
     2461 cggttgcccc catcaactcg
//
LOCUS       DATANS010000708         2269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_371876, whole genome shotgun sequence.
ACCESSION   DATANS010000708 DATANS010000000
VERSION     DATANS010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371876"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VHT22_10555"
     CDS             <1..841
                     /locus_tag="VHT22_10555"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEX3138041.1"
                     /translation="NAITEQLESLDFASTPGHVLNGLKREELVALNSTLLHELYFASM
                     GGEGQPTKGMTEILARDFGSIDRWRAEFSAMGCALGGGSGWVLLTYIPRDGRLINQYA
                     AEHSQAVAGGIPILALDMYEHAYHMDFGANARAYVDTFLRNVDWPALEMRYEDAAKVP
                     GPRPVEQPEFGDVPGIGVEEVRQMMAAGTPVQVIDVRPRAAVSRLQDIAEGVQWRDPE
                     KLQQWMGELSKTEPVVVYCAYGFHVGCKTAIKLREAGFDAKYMNSGHSGWKAIGAPVK
                     MNA"
     gene            complement(927..1676)
                     /locus_tag="VHT22_10560"
     CDS             complement(927..1676)
                     /locus_tag="VHT22_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX3138042.1"
                     /translation="MAAGAKIIRYRGVIKRYGGFQALGGVDLDVHAGEVVCLIGPSGS
                     GKSTLLRCTNGLEAIDGGDIEFEGAMLPRDERALRAVRTRMGMVFQSFELFPHLTALE
                     NVAEGPRTVLRNDLVEANRRATAMLAKVGLADKASSYPSALSGGQQQRVAIARALAME
                     PDVMLFDEPTSALDPETIGEVLSVMKKLADEGMTMVVVTHEMTFARRVGDWVAVFDTG
                     RIVEQGPPTQIFDAPTTERTRDFLSHLGWTG"
     gene            complement(1703..>2269)
                     /locus_tag="VHT22_10565"
     CDS             complement(1703..>2269)
                     /locus_tag="VHT22_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX3138043.1"
                     /translation="IAVPCGIIVAILRLYGWAPVRALATAYVEFFRNLPLILVVFWAF
                     YVPPILTGLGLSPLATGLAALALNVTAYNAETFRAGINSIRRGQVEAAMVLGMNRVQA
                     LRRVVVPQAVRRILPVLASTWVSLFKDTSLVSVIAVTELAYVSMQIRAQSFRVLEMLT
                     AMAAIYWLLGYPQAKLVDWIQRRFAITE"
BASE COUNT          434 a          716 c          749 g          370 t
ORIGIN      
        1 caacgccatc actgagcagc tcgagtcact cgacttcgcg agcacgcccg gccatgttct
       61 taacgggctc aagcgcgaag agctggtcgc gctcaactcg acgctgctgc acgagctcta
      121 tttcgcgagc atgggcggcg aaggccagcc aacgaagggc atgaccgaga tcctcgcgcg
      181 cgacttcggc tcgatcgacc gctggcgcgc ggagttcagc gcgatgggat gcgcccttgg
      241 cgggggctca ggctgggttc tgctgaccta cattccgcgc gacgggcgcc tcatcaatca
      301 gtatgctgcc gagcactcgc aagcggtcgc aggcggaata ccgatcctcg cgctcgacat
      361 gtacgagcat gcgtaccaca tggacttcgg cgccaacgcc agggcatacg tcgatacgtt
      421 cttgcgcaac gtggattggc cggcgttgga gatgcgctac gaagacgcgg cgaaagttcc
      481 tggtccgcgg cccgtcgaac agccggagtt cggcgatgtc ccggggatcg gcgtggagga
      541 agtgagacag atgatggccg cggggacgcc tgtacaggtc atcgacgtgc gcccgcgggc
      601 ggccgtctcg cgcctgcaag acatcgccga gggcgtccag tggcgcgatc ccgagaagct
      661 ccagcaatgg atgggtgagc tgtcgaaaac agaaccggtc gtcgtgtact gcgcgtacgg
      721 ctttcacgtc gggtgcaaga ccgcgatcaa gctgcgcgag gcgggcttcg atgcgaaata
      781 catgaatagc gggcactccg gctggaaggc gattggcgca ccggtcaaga tgaacgcatg
      841 atccccggtt ggcgcgacat ggcgccaccg ggattcggca gggggaaaat gctccgccag
      901 tggagtgaag cggagatcga gagcgcctag cctgtccacc ccaggtggct caggaaatcg
      961 cgcgtgcgct cggtcgtcgg cgcgtcgaag atttgcgtgg gcggcccctg ctcgacgatg
     1021 cggccggtgt cgaaaaccgc aacccagtcg ccgacccgcc gcgcgaaggt catctcgtgc
     1081 gtcacgacga ccatggtcat gccttcgtcg gcgagttttt tcatcacgct gagcacctcg
     1141 ccgatcgtct cgggatcgag tgcggacgtc ggctcgtcga acagcatgac atcgggttcc
     1201 atcgccaggg cgcgggcaat cgcgacgcgt tgctgctgac cgccggacag ggccgatgga
     1261 tagctcgacg ctttgtcggc aagaccgacc ttggcgagca tcgccgtggc gcgacggttg
     1321 gcttcgacga gatcgttgcg cagcaccgtg cgcggtcctt cagcgacgtt ctccagcgcc
     1381 gtcaaatgcg gaaacagctc gaagctctgg aagaccatcc ccatgcgcgt tcgcacggcg
     1441 cgcagtgcac gctcgtcgcg tggaagcatc gctccctcga actcgatatc gccgccgtcg
     1501 atcgcttcga gtccgttggt gcagcggaga agcgtcgact tgcccgagcc ggacggtccg
     1561 atcaggcata caacctcccc ggcgtgcacg tccaggtcga cgccgcccaa cgcctgaaaa
     1621 ccgccgtagc gcttgatcac gccgcgatag cggatgatct tggcgccggc ggccatcggt
     1681 cgtagcggtg acgtgtctgc gttcattcgg ttatggcaaa gcggcgttgg atccagtcga
     1741 cgagctttgc ctgcggataa ccgagcagcc agtagatcgc agccatcgcg gtcagcattt
     1801 ccagcacgcg gaagctctgc gcgcggatct gcatcgagac gtacgcgagc tcggtaaccg
     1861 caatcaccga caccagcgac gtgtccttga acagcgatac ccaggtgctc gccagtaccg
     1921 gcaggatgcg gcgaaccgcc tgcggcacga cgacgcggcg cagggcctgc actcggttca
     1981 tccccaacac cattgccgcc tcgacctggc cgcggcgaat cgaattgatg ccggcgcgaa
     2041 acgtttccgc attgtacgcc gtgacgttga gtgccagagc cgcgaggccc gtcgcgagcg
     2101 gggacaagcc gagcccggtc agaatcggcg gcacgtagaa cgcccagaag acgacgagga
     2161 tcagcggcag gttacggaag aattcgacgt acgccgtcgc gagggcgcgc accggagccc
     2221 agccatagag gcgcaatatc gccacaatga tgccgcaagg cacggcaat
//
LOCUS       DATANS010000709         1667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_119295, whole genome shotgun sequence.
ACCESSION   DATANS010000709 DATANS010000000
VERSION     DATANS010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119295"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VHT22_10570"
     CDS             <1..449
                     /locus_tag="VHT22_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX3138044.1"
                     /translation="FHFFNQYRASGKVRLFEGSGGYADGEQRRDFVSVEDAVAVNLYF
                     LDHPRQSGIFNVGTGAAQSFNDVAVATVNACRRHAGETPLALADMQRQGVIEYIPFPP
                     ELAGKYQSYTQADISALREAGYAAPLMTVEEGVARYIETLIARAHT"
     gene            455..1342
                     /gene="cysM"
                     /locus_tag="VHT22_10575"
     CDS             455..1342
                     /gene="cysM"
                     /locus_tag="VHT22_10575"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HEX3138045.1"
                     /translation="MDKTLEDFIGNTPLVRLQRLPGETRNVLLAKLEGNNPAGSVKDR
                     PALSMIKRAHQRGEIKPGDTLIEPTSGNTGIALAMAAAMMGYHMVLVMPEHLSIERRQ
                     SMAAFGAEIVLTPQTGGMEMARDVAEKMRDEGKGVILDQFANPDNPLAHYESTGPEIW
                     RDTEGKITHFVSSMGTTGTIMGVSRFLKEKNPAVQIVGCQPTDGSQIPGIRKWPAAYL
                     PKICDWSRVDRVIEVAQADAEDMTRRLAREEGIFAGVSSGGAMWTALQISAEVSDAVI
                     VTIICDRGDRYLSTGIFPA"
     gene            1455..>1667
                     /locus_tag="VHT22_10580"
     CDS             1455..>1667
                     /locus_tag="VHT22_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138046.1"
                     /translation="MLTANRITGVLRVGATTALELKATEVVMAGQTWRNVRIACPDLK
                     QERDQLICAQGALETPARLPLSFRYST"
BASE COUNT          322 a          529 c          539 g          277 t
ORIGIN      
        1 cgttccattt tttcaatcag tatcgcgcga gcggcaaggt caggctgttc gagggcagcg
       61 gcggttacgc cgacggtgag cagcggcgtg atttcgtgtc cgtcgaagac gcggtcgccg
      121 tcaatctcta cttcctcgat catccgcggc agtcgggcat cttcaacgtg ggtacgggcg
      181 ccgcgcaaag cttcaacgac gtcgccgtcg cgacggtcaa cgcctgccgc cggcatgccg
      241 gtgagacgcc gctcgcgctg gcggacatgc agcgccaggg cgtgatcgag tacatcccgt
      301 tcccgccgga actcgcgggc aaatatcaaa gctataccca ggccgacatc agcgcgctgc
      361 gcgaggccgg ttatgccgcc ccgctcatga ccgtggagga gggagtcgcg cgctatattg
      421 aaacgctcat cgcgcgggca catacttgag tgccatggac aagacgctcg aagacttcat
      481 cggcaacacg ccgctcgtcc ggctgcaaag gctgcccgga gaaacgcgca acgtgctgct
      541 cgccaagctg gagggcaaca acccggccgg ctcggtcaag gaccggcccg cgctgtcgat
      601 gatcaagcgc gcgcaccagc gcggcgaaat caagcccggc gatacgctaa tcgagccgac
      661 cagcggcaat accggcatcg cgctcgcgat ggccgctgcc atgatgggtt atcacatggt
      721 gctggtaatg cctgagcacc tgtcgatcga gcggcgccag tcgatggcgg cgttcggcgc
      781 cgagatcgtg ctgacgccgc aaaccggcgg catggaaatg gcgcgcgacg tggccgagaa
      841 aatgcgcgac gaaggcaagg gcgtgatcct cgaccagttc gccaatccgg acaatccgct
      901 cgcccactac gaaagcacgg gcccggaaat ctggcgcgat actgaaggca aaatcacgca
      961 cttcgtctcc agcatgggca ccaccggcac aatcatgggc gtgtcgcgtt ttctcaagga
     1021 aaaaaatccg gcggttcaga tcgtcggctg ccagccgacc gatggctcgc agattccggg
     1081 catccgcaag tggccggcgg cttatctgcc gaaaatctgc gactggagcc gcgtggaccg
     1141 agtgatcgag gtcgcgcagg ccgacgctga agacatgacg cggcgcctcg cccgcgaaga
     1201 gggcatattc gccggcgttt cttcgggcgg cgccatgtgg acggcgttgc agatctcggc
     1261 ggaagtcagc gatgcggtga tcgtcactat catctgcgac cgcggcgacc gctatttgtc
     1321 gacggggatc tttcctgcct gagctgcggg ccggaatcgg cgcgcaagcc ggcgcggcac
     1381 tgatcagcct cctgctcacc ggctctgcgc cagcccagca aatcggcatc agcatagagc
     1441 gcatcgacag tccggtgctg actgcgaacc ggattacggg cgtgttgcgc gtgggtgcga
     1501 ccaccgcgct cgagctcaaa gccaccgaag tcgtcatggc cggccagacg tggcgcaacg
     1561 tgcgcatcgc gtgtcccgat ttgaaacagg agcgcgatca actcatttgc gcgcagggcg
     1621 cgctggagac acccgcgcgt ctgccgttga gttttcgtta ctcgacg
//
LOCUS       DATANS010000710         5021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_539454, whole genome shotgun sequence.
ACCESSION   DATANS010000710 DATANS010000000
VERSION     DATANS010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539454"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VHT22_10585"
     CDS             complement(<1..569)
                     /locus_tag="VHT22_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HEX3138047.1"
                     /translation="MNAAAAPPAVPPDSHGVAVAAERRWAFIVGIIILLLVAMMIFTG
                     LQWAAMPPSRVETVDPRTLHVAGEFVESNLGTSVGPDGKVIARLIAQQYSFEPACIVV
                     PAETPVTFRGTSTDVVHGFIVGTTNANVMLVPGFVATFTTTFPKTGEHLMPCHEYCGT
                     GHEAMWGRVRVLPAEEFLNKTRGGERLSC"
     gene            complement(566..733)
                     /locus_tag="VHT22_10590"
     CDS             complement(566..733)
                     /locus_tag="VHT22_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011355005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138048.1"
                     /translation="MPTQSDDARSDAEVDEIVRRGPSGAFALAGIAAAIVVVLYFAFY
                     FLVYLPRGAVQ"
     gene            complement(744..1949)
                     /locus_tag="VHT22_10595"
     CDS             complement(744..1949)
                     /locus_tag="VHT22_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX3138049.1"
                     /translation="MLPQTSPTATATSTTTQFLDRGEYLARAGDCVACHTAPEGREFA
                     GGRAMPTPFGNLYVPNITPDDETGIGKWSGDDFYRMMHTGISRDGSLMYPAMPFASYT
                     RVTREDSDAIYGYLMSVPPVQQKNRRHELRFPFNQRDLLVGWRALYFKEGEYVPDPKQ
                     SAQWNRGAYLVDGLGHCAMCHTAINRLGGSRESDAFEGGMIPNQNWYAPSLTSGREAG
                     LGHWSIKDITDLLQVGVSNRATVYGPMAEVVYNSLQYLSDEDVEAMAVYLKALPQRDA
                     EAPPTSQARLVSPDVMESGRKVYVTQCAMCHGAEGKGQPPQFPPLAGNQSITMASPVN
                     SIRMVLNGGYPPGTRKNPRPHGMPPFAHILDDAEAAAVVTYIRTAWENAGTPVTPAQT
                     NELRTLLPE"
     gene            complement(2033..2668)
                     /locus_tag="VHT22_10600"
     CDS             complement(2033..2668)
                     /locus_tag="VHT22_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEX3138050.1"
                     /translation="MPAFKTLDSMEARVQGCVTCHGQSGQGTLSGAFPRIAGKPAGYL
                     YNQLIAFRDGARQYPPMNYLVAYLPDAYLREMAEYYAKQRPPFEGKDATAVDPKLVAR
                     GHDIATAGDASKGIPACVACHGRRLTGMEPGIPGLAGLRANYIAAQLTRWRVGSRHAA
                     EPDCMKRIASRLSESDVAAVSVWLAQQEGPKDASPESQNLVRMPLACGSQR"
     gene            2970..3563
                     /locus_tag="VHT22_10605"
     CDS             2970..3563
                     /locus_tag="VHT22_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201021.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HEX3138051.1"
                     /translation="MATTNTPAISRYPLRKLEDLPEDIRARMLEVQEKAGFIPNVFLT
                     LAHRPDEFRAFFAYHDALMLRDSGLSKAEREMIVVATSGANDCLYCIVAHGAILRIYA
                     KNPLLADQVAANYRKADISARQKAMLDFALKVALRSVELVDADYERLRDHGFTDEDIW
                     DIGAIAAFFALSNRMANLIGMRPNDEFHLLGRLPRKA"
     gene            3644..3889
                     /locus_tag="VHT22_10610"
     CDS             3644..3889
                     /locus_tag="VHT22_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138052.1"
                     /translation="MNRYNVVVAAVAFAALAGCATTPTSPAEEQPDDKAYVTGSRLPV
                     RDNSTSASVKGVANKNAIDDMMNRGSTIYIPPKGTTN"
     gene            complement(3915..>5021)
                     /gene="cysC"
                     /locus_tag="VHT22_10615"
     CDS             complement(3915..>5021)
                     /gene="cysC"
                     /locus_tag="VHT22_10615"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX3138053.1"
                     /translation="DELVFSPSGRSAKVAELLAWPDAAPDQVEAGGNASIVLDRPLVV
                     ERGDMASHHEHAPKLTAVFDAEIFWLSATPLIPGRELTMQLGTRSAGIRVQAIRHRID
                     VATLAPELASTIAAGEIASVTLRAHELIAVDDFAAMPATGRFVLRDAYVTVGGGIIDA
                     SGYPDHRAMPERARNLTPMRHQVTETERASRFGHVGAVVWLTGLSAAGKSTLAMALER
                     RLFDGGYATYVLDGDNVRRGLNANLGFSPEDRQENIRRVGEAAALFAEAGFVCITAFI
                     SPYRDDRRRARAAVAKGHFLEVYVKADLAACEARDPKGLYRRAREGELKDFTGIDSPY
                     EEPQAADLILDTTQEDIAACVDKLTEFVAARCRV"
BASE COUNT          884 a         1654 c         1622 g          861 t
ORIGIN      
        1 tcacagctga gcctctcgcc gccgcgggtc ttgttcagga attcctcggc cggcagcacc
       61 cgcacgcggc cccacatcgc ctcgtggccg gtgccgcagt actcgtggca aggcatcagg
      121 tgctcgccgg tcttgggaaa cgtcgtggtg aacgtggcca cgaagcccgg caccagcatg
      181 acgttcgcgt tggtcgtgcc gacgatgaag ccatgcacga cgtccgtgct cgtgccgcgg
      241 aacgtgacgg gcgtttcggc gggaacgacg atgcatgccg gctcgaacga atattgctgg
      301 gcgatgaggc gcgcgatcac cttgccgtcg ggaccgacac tcgttcccag attgctttcg
      361 acgaactctc cggcgacgtg aagggttcgc gggtcgaccg tctcgacgcg cgacggtggc
      421 atcgccgccc attgcaaccc ggtgaaaatc atcatcgcca ccagcagcag aatgatgatg
      481 ccgacgatga acgcccaccg gcgttcggca gcgacggcga ccccatgcga gtcgggcggt
      541 acggcgggag gcgccgcagc ggcgttcatt gcacggcccc tcgcgggagg tagaccagga
      601 aatagaatgc gaaatagagc actaccacga tcgccgcggc gatgcccgcc agcgcaaagg
      661 cgccggaggg accgcggcgg acgatttcgt cgacctcggc gtcgctgcgg gcgtcatcgc
      721 tctgtgttgg catcacggtc tcctcattcc ggcagaagcg tgcggagctc gttcgtctgc
      781 gcgggcgtga ccggcgtacc ggcgttctcc cacgccgtgc ggatataggt caccacggcg
      841 gcggcctcgg cgtcgtccag gatatgcgcg aacggcggca tcccgtgtgg gcgcggattc
      901 ttgcgcgtgc ccggcggata gccgccgttc aagaccatgc gaatggagtt cacgggcgag
      961 gccatggtga tcgattgatt gccggccagc ggcggaaatt gcggcggctg tcctttgccc
     1021 tccgcaccgt gacacatcgc gcactgcgtt acgtaaacct tgcggccgct ttccatgacg
     1081 tccggactga ccagtcgcgc ctggctcgtc ggcggcgcct cggcgtcgcg ctggggcagc
     1141 gctttgagat acaccgccat ggcttccacg tcctcgtcgg agaggtactg aaggctattg
     1201 tagacgacct cggccatcgg gccgtagaca gtagcgcgat tcgagactcc gacctgcaag
     1261 aggtcggtga tgtccttgat gctccagtgt ccgaggcccg cttcgcggcc gctggtgagc
     1321 gacggcgcgt accagttctg gttcggaatc attccgcctt cgaaggcgtc cgactcgcgt
     1381 gatccgccga gcctgttgat ggcggtgtgg cacatcgcac agtggccgag gccatccacc
     1441 agataagcgc cgcggttcca ttgcgccgat tgctttggat ccgggacgta ttcgccctcc
     1501 ttgaagtaca aggcgcgcca gccgacgaga agatcgcgct ggttgaacgg aaaacgcagc
     1561 tcgtgccgcc ggtttttctg ctggaccggc ggcaccgaca tcaggtaccc gtagattgcg
     1621 tccgagtcct cgcgggtgac tctggtatat gacgcgaatg gcatggcggg atacatcagc
     1681 gagccgtcgc gcgagatccc ggtgtgcatc atgcggtaga aatcgtcgcc gctccattta
     1741 ccgatgccgg tctcatcgtc gggcgttatg ttcggcacgt aaaggttgcc gaacggcgtg
     1801 ggcatggcac gcccgccggc gaactcgcgg ccttccggcg ccgtgtggca cgccacgcag
     1861 tcgccggcgc gggccagata ctcgccgcga tcgaggaatt gcgtggtcgt gctggtcgcc
     1921 gtcgcggtcg ggctggtctg cggcaacatg cccggtcgaa gcagcgccag gccgatggcg
     1981 ccgagaatca ccagcacgac cgcgacaata gcgatgcgaa ggccgattcg catcagcgct
     2041 ggctcccgca cgcgagcggc atgcgaacga gattctgcga ttcgggcgat gcgtctttcg
     2101 gtccttcctg ctgcgccagc cacaccgaga ctgccgcgac gtcggactcg gacagccgcg
     2161 atgcaatgcg cttcatgcag tcgggctctg ccgcgtgacg actgccgaca cgccagcgcg
     2221 tgagttgggc ggcgatgtag ttggcacgca gcccggcgag ccccgggatc ccgggctcca
     2281 tgcctgtcaa ccgcctgccg tgacaggcca cgcaggccgg aatgcccttg ctcgcatcgc
     2341 ccgcggtcgc gatgtcgtgg ccgcgagcca ccagcttcgg atcgaccgcc gtcgcatcct
     2401 tgccctcgaa tggcggacgc tgcttcgcgt agtactccgc catttcgcgg agatacgcgt
     2461 ccggcagata cgcgaccagg tagttcatcg gagggtactg tctcgcccca tcccgaaacg
     2521 cgatcagctg gttgtagaga tagcccgcgg gcttaccggc gatccgggga aaggcgccac
     2581 tcagggtgcc ttgcccgctc tgcccgtggc aggtcacgca cccttggacc cgggcctcca
     2641 tcgagtcgag ggtcttgaac gccggcaccg cttcgttctg cgcccagccg gccgcagcgt
     2701 ggaggcaaca cacagcaagc acagacgcgg cccaggtcgg ccgcggcagc gcacgcgcac
     2761 gtcggttaga tggcgtcact cgaggaacgg ggccaaagcg ccgatgggca accagcgcag
     2821 agtctaccac cgctcggccg tgaccgacgc agacaacatg aaccggtcgg tactccgaca
     2881 aatcggcatg cccatgtcgg atcgacgcag gcgtcgaacg caacaagatc gaacaaggac
     2941 agtcgggtgg acggcgaacg gaagagctta tggcaacgac caacacaccg gcgatcagcc
     3001 gctatccgct tcggaaactc gaagacttgc ccgaagacat ccgcgcgcgg atgctcgagg
     3061 tgcaggagaa agccggcttc attcccaacg ttttcctgac gctcgcccat cgccccgacg
     3121 agttccgcgc ttttttcgcc taccacgatg ctctgatgct gcgcgattcc ggcttgagca
     3181 aggccgagcg cgagatgatc gtcgttgcca catctggcgc caacgattgc ctgtattgca
     3241 tcgtcgcgca cggcgcgatc cttcgcattt atgccaagaa tccgctgctc gccgaccagg
     3301 tcgcggccaa ctatcgcaag gcggacatca gcgcgaggca gaaggcgatg ctcgacttcg
     3361 cgctcaaagt cgcgctgcgg tcggtcgagc tcgtcgatgc cgattacgag cggctgcgcg
     3421 accacggatt caccgatgaa gacatctggg acatcggggc gatcgccgcg ttttttgccc
     3481 tgtccaaccg gatggccaac ctgatcggca tgcggccgaa cgacgaattc caccttctcg
     3541 gacgattgcc ccgaaaggcg taggcaggac agggctcgcg ctagcgcccg acctcggata
     3601 aacttccgtt ttcgccgcag ctgcggccct cgaggaaagc taaatgaatc gctacaacgt
     3661 ggtcgttgcc gccgtcgcct tcgcggctct tgctggctgc gcgaccactc ctacatcgcc
     3721 ggctgaagaa caaccggacg acaaggcgta cgtcaccggc agtcgcttgc cggtgcgcga
     3781 taacagcacg tcggcaagcg tcaagggcgt cgccaacaag aatgccatag acgacatgat
     3841 gaatcgcggc agcacgatct acattccgcc caaaggcacc acgaactaga gcgaggcgaa
     3901 tacgctgccg gccgctagac ccggcagcgg gctgcgacga attcggttag cttgtcgacg
     3961 caggcggcga tgtcctcttg cgtcgtatcg aggatgaggt cggccgcctg tggctcttcg
     4021 taggggctgt cgatgccggt gaaatccttg agctcgcctt cgcgcgcccg ccggtagagg
     4081 cctttcgggt cgcgcgcctc gcatgcggcg aggtcagcct tgacgtagac ctcgaggaaa
     4141 tgccctttgg ccaccgcggc ccgcgcccgg cgcctgtcgt cgcggtaggg tgagatgaac
     4201 gcggtgatgc agacgaaccc ggcttcggcg aacaaggcgg cagcctcgcc gacgcggcgg
     4261 atattctcct gtctgtcctc aggcgagaag cccaggttcg cgttcaagcc tcgccgcacg
     4321 ttgtctccgt cgagcacgta agtcgcgtag ccgccgtcga acaggcgccg ctccagcgcc
     4381 atcgccagcg tcgatttccc cgcggccgac aagccggtca gccagacgac cgcccctacg
     4441 tgcccgaacc gtgaagcgcg ctcggtctcg gtcacctgat ggcgcatcgg cgtaagattc
     4501 cgcgcgcgtt ccggcattgc gcgatgatcg ggatagccgc tggcgtcgat gatgcccccg
     4561 ccgaccgtga cataagcgtc gcgcaggacg aaacgcccgg tcgccggcat cgccgcaaaa
     4621 tcgtcgacgg cgatcagctc gtgcgcacgc agggtcacgc tggcgatttc tccggccgcg
     4681 atcgtgctcg ccagttccgg cgccagggtc gccacgtcga tgcgatggcg gatcgcctgc
     4741 acgcgtatcc cggcgcttcg tgttcccagc tgcatcgtca gctcgcggcc cgggatcaat
     4801 ggcgttgccg aaagccagaa gatttcagcg tcgaataccg ccgtcagctt tggcgcgtgc
     4861 tcgtgatgac tcgccatgtc gccgcgctcg acgaccagcg gccggtcgag aacgatcgaa
     4921 gcattgccgc cggcctcgac ctgatcaggc gcggcgtcgg gccacgccag caattcggcg
     4981 accttggcgg agcgccccga aggcgaaaac accagctcgt c
//
LOCUS       DATANS010000711         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_498143, whole genome shotgun sequence.
ACCESSION   DATANS010000711 DATANS010000000
VERSION     DATANS010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498143"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(393..1598)
                     /locus_tag="VHT22_10620"
     CDS             complement(393..1598)
                     /locus_tag="VHT22_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HEX3138054.1"
                     /translation="MATTLAEVLRHFGPAYLGSHGLSTAQARAWRAIVSCRTPALGGE
                     RLRCDGCGAEQWRWHSCRNRHCPQCQSRARDAWRVARLAELLDVPYCHLVFTLPHELN
                     ALAAVHAHWVYDTLMRCAAATLSEFAANPRWLGGVGSFTLVLHTWTQDLRRHLHVHAL
                     MACGALRQGDSDGAGHSAWIEPRRASNFLFPVHALSRVFRGKFMHSLQGAMQAGGLPR
                     DPANTDALRRRRMRALRRHDWVVYAKTPLAGPAAVLDYLARYTHRTAIGNERLVDINA
                     GKVRLRVRGDENGARRTIALDGAQFIGRLLQHVLPPGFKRIRHYGLLAPATKADRLRR
                     ARQLLAMPAANPQACEDAQAFMRRVAAIDVDCCPHCSIGRWRVLQHVAADRPALNSIT
                     TPTACRGPP"
     gene            complement(1605..2174)
                     /locus_tag="VHT22_10625"
     CDS             complement(1605..2174)
                     /locus_tag="VHT22_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX3138055.1"
                     /translation="MLSREELARLFAAARPLKSRTFLMLSYGTGLRLSEMCHLRVADI
                     DSHADRMCIRVEQGKGAKDRYVPLSEDVLQLLRTWWRSAHPREWLFGAARDPARPLNL
                     KSAQRWYYAGCAHAGITKRGGIHSLRHAYATHLLEAGVDLYSLQQWLGHNHVSTTTRY
                     LHLARPDAPDGARRKPLELLGALTPSTTH"
     gene            complement(2175..>2436)
                     /locus_tag="VHT22_10630"
     CDS             complement(2175..>2436)
                     /locus_tag="VHT22_10630"
                     /inference="COORDINATES: protein motif:HMM:NF024886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX3138056.1"
                     /translation="RGMADRTQESYIDAVSRLARHYRRSPDTLSAEEVQRYLLHLLRD
                     RNLSRSSVNQYGCAFRFLYGTVLGLNGDSFQIPLAPAPHRLP"
BASE COUNT          410 a          788 c          861 g          377 t
ORIGIN      
        1 aggcaaagcg cgtctggttc accggcctca cgaacaaaga tgtcgccact catgggacct
       61 aacgaacagc gtttatctga cgccgggccg atgtgtgtcg aaggccgtga cggtgcatgg
      121 cgtcagataa acctcgttgg actgaaccgc cgatatggcg gtgcgctatg ggcagtgtaa
      181 gtttcggcgg tgggctgggg caacaccgtc gtgctgcaga cgaactcgat agcggtggtg
      241 gatggtcaat gtcgtgttcg aatggcgctc ggagtcgagt ttgccggcac tgcgcgtggc
      301 tcgatacctg gtaggggcga agcagacgcg atacggtccg ggcctgcgtc gacgacgaga
      361 ccttgcaaga gacagccgcg tgcccgaatg catcaggggg gcccccgaca cgccgtcggc
      421 gtggtgatcg agttcaacgc cggccgatca gccgccacgt gctgcagtac tcgccagcgt
      481 ccgatactgc aatgcgggca gcagtcgaca tcgatggcgg ccacgcgccg catgaaggcc
      541 tgcgcgtcct cgcacgcctg gggattggcc gccggcatcg ccaggagttg gcgagcccgc
      601 ctcaggcggt ccgctttggt cgccggcgcg agcagcccgt agtggcggat gcgcttgaag
      661 cccgggggca agacatgctg cagcagtcgg ccgatgaact gcgcgccgtc cagcgcgatc
      721 gttcgtctgg cgccgttctc gtcgccgcgc acccgcagcc ggaccttgcc ggcgttgatg
      781 tccacgagcc gctcgttgcc gatagccgtg cggtgcgtgt agcgcgccag gtagtcgagc
      841 actgctgcag gaccggccag cggggtcttg gcgtaaacga cccagtcgtg ccggcgcaga
      901 gcccgcattc gccgccgccg caaggcatcg gtgttcgccg gatcgcgggg cagccctccg
      961 gcttgcatcg caccttgcag cgaatgcatg aacttgcctc tgaacacccg cgagagcgcg
     1021 tgcacgggga acaagaagtt gctcgctcgc ctgggctcga tccaggcgct gtggccagcg
     1081 ccgtcgctgt ccccctgccg cagcgcgcca caggccatca gcgcgtgcac atgaaggtgg
     1141 cgacgcaggt cctgcgtcca ggtgtgcaac accagcgtga aggagccgac gccgcccagc
     1201 cagcgcgggt tggccgcgaa ctcgctgagt gtggccgccg cacaccgcat cagcgtgtcg
     1261 tagacccagt gcgcgtgcac ggcggccagc gcgttgagct cgtgcggcag cgtgaagacg
     1321 aggtggcagt acggcacgtc cagcagttcg gccagccggg cgacgcgcca ggcatcgcgg
     1381 gcgcgactct ggcattgcgg gcaatggcga ttgcggcacg aatgccagcg ccactgctcg
     1441 gcaccgcacc cgtcgcagcg caggcgctcg ccgccgagtg ccggcgtccg gcacgacacg
     1501 atggcgcgcc aggcccgtgc ctgcgctgtg ctcagtccgt gcgagccgag ataggccggg
     1561 ccgaagtgcc gcagcacttc cgccagcgtc gtcgccacgc ccgatcagtg cgtcgtgctc
     1621 ggcgtcaagg cgccgagcag ctccaggggc ttgcgccggg caccatcggg tgcgtcggga
     1681 cgtgccaggt gaaggtagcg cgtcgtcgtg gagacgtggt tgtggcccag ccattgctgc
     1741 aggctgtaga gatcgacgcc cgcttccagc agatgcgtcg cgtacgcgtg gcgaagcgag
     1801 tgaatgccgc cgcgcttggt gatgccggcg tgtgcacacc ctgcgtagta ccagcgctga
     1861 gcggacttga ggttcagggg ccgcgccgga tcgcgcgcag cgccgaacaa ccactcgcgg
     1921 ggatgggcgc tgcgccacca agtgcgcagc agctgcaata cgtcttcgga cagaggcacg
     1981 tagcggtcct tggcgccctt gccctgctcg acgcggatgc acatgcggtc ggcgtggctg
     2041 tcgatgtcgg ccacgcgcag atggcacatc tccgacaggc gcagacccgt gccgtacgac
     2101 agcatcagga aggttcgcga cttcaagggc cgcgccgctg caaacaggcg tgccaactcc
     2161 tcgcgcgaga ggatctaggg aagtcgatgc ggtgccggag ccagcgggat ctggaacgaa
     2221 tccccgttca agccgagcac cgtgccgtac agaaagcgaa aggcgcagcc gtactggttg
     2281 acgctcgagc gcgagagatt gcggtcgcgc agcaagtgca gcaggtagcg ctgcacctct
     2341 tcggccgaca aggtatcggg actgcgccga tagtgccgcg ccagtcgcga cacggcgtcg
     2401 atgtaggact cctgagtgcg atccgccatg ccgcgc
//
LOCUS       DATANS010000712         3359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_243546, whole genome shotgun sequence.
ACCESSION   DATANS010000712 DATANS010000000
VERSION     DATANS010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243546"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="VHT22_10635"
     CDS             <1..1121
                     /locus_tag="VHT22_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HEX3138057.1"
                     /translation="MMPYFISIGWLQPNEPFRKITFLIALGMIMGAAIVDVSLILFRA
                     WQRSRQVKAEVVEQEDWKKTNTRRLVAWVVFWGICIVVVGSQVLHQPVGYLVMAVLLV
                     FVFALVNGISLGITDSNPISSAFVVTVVLMAGIGLRDPIVGMMAATVLLVSTSEACDM
                     QQDRSTGWRLGTNRMIQFRFQVAGIVMGAIMAVVFAKLFMSAYPVLGLDQTTMPADQQ
                     PAQWSSAMTYKFVGVLRSLTDDKPYQRTAIWVGILIGFVTEFLRKYIKSRERYQAFVK
                     SGRVGFSTDFVLDAIVLPSPYASSFGGFLNLMTSAWFAAGGVLSSFINSLPKRKDKPA
                     ALPSDMSTTSLVGGGLIAGDALAALGLGIAGLLGTLFG"
     gene            1323..2243
                     /locus_tag="VHT22_10640"
     CDS             1323..2243
                     /locus_tag="VHT22_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX3138058.1"
                     /translation="MLNPLNPARSTAIRFHIGIDGGGTHTRARLASTDRAVLGFAASG
                     PSALGQGIEQAWQHIVQAVDGAFADAGLAPAPREQCALGLGLAGANVRQRCEEFFRRA
                     PPFAEIALDTDAYTTLLGAHEGRPGAIVAAGTGSVGEALRRDGLRVAVGGWGFPVGDE
                     GSGAWLGLRAMRETHRAIDGRAHVGPLVGAVLAVAGTSREALLGWCDRPGQNAYAALA
                     PLVFDAAATDPFAAALLDRAARSLEEIAAALDPERVLPLAIFGSIGQRLLPRFEPPIQ
                     ARCVEPAGDSADGALRLVGQALSELGGSER"
     gene            2240..2980
                     /locus_tag="VHT22_10645"
     CDS             2240..2980
                     /locus_tag="VHT22_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3138059.1"
                     /translation="MKGRPFEFRVDARAASPLYMQLAQKLAQAIRNDDYQPNEALPSE
                     RVLSESLSLSRVTARKAIDQLVEQGLVVRRRGSGNYIAPRVEQPLSRLTSFSEELHRR
                     GFTPSSRWLARGCTAAAPDEQLSLGLSPGSRVARLERLRLADNVVMAYEVSVLPEQVL
                     PDPSQVDASLYAHLAMLGRAPVRALQHLRALNADARLAQLLEVPTGRAVLFITRVGYL
                     DTGRAVELTHSYCRSDYYDFVAETRRQA"
     gene            2977..>3359
                     /locus_tag="VHT22_10650"
     CDS             2977..>3359
                     /locus_tag="VHT22_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3138060.1"
                     /translation="MSALRIEGHILTPQGFVRGVLEHEEGRITRVEGTLRTEDQARSE
                     AQGLVLPGFIDTHVHGGGGSDTMEGGEAIGRIARLHARHGTTSLLATTMTAPLAEVEA
                     AMRALASACSHRASGAARVLGVHLEG"
BASE COUNT          529 a         1078 c         1162 g          590 t
ORIGIN      
        1 cgatgatgcc gtatttcatc tccatcggat ggctgcagcc gaacgagccg ttccgaaaga
       61 tcacgttcct gatcgccctc ggcatgatca tgggcgctgc gatcgtagac gtgtcgctga
      121 ttctcttccg cgcctggcag cgctcgcgcc aggtcaaggc ggaggtagtc gagcaggagg
      181 attggaagaa aacgaatacc cggcgactcg tcgcctgggt cgtgttctgg ggtatctgca
      241 tcgtcgttgt cggttcgcag gtcctgcacc agccggtcgg ctatctggtg atggcagtgc
      301 tgctcgtatt cgtgttcgcg ctggtcaacg ggatatccct cggcatcacc gactcgaacc
      361 cgatttcctc cgcgttcgtc gtcaccgtgg tcctgatggc gggcatcggc ttgcgcgatc
      421 cgatcgtcgg gatgatggcg gcgacggtcc tgttggtgtc gaccagcgaa gcttgcgaca
      481 tgcagcagga ccggtcgacg ggctggcggc tcgggaccaa ccggatgatc cagttccgct
      541 tccaggtggc cggcatcgta atgggcgcga tcatggccgt ggtgttcgcc aagctcttca
      601 tgagcgcgta ccctgtgctg ggcctcgatc agacgacgat gcccgccgac cagcagcccg
      661 cgcaatggtc gtcggcgatg acctacaagt tcgtcggcgt gctgcgcagc ctcaccgacg
      721 acaagcccta ccagcgtacg gcgatctggg tcggcatcct gatcggattc gtgaccgagt
      781 tcctgcgcaa gtacatcaag tcgcgcgagc gataccaggc gttcgtgaag tcgggccggg
      841 tggggttcag taccgatttc gtcctcgacg ccatcgtgct tccgtcgccc tatgcgtcgt
      901 cgttcggcgg attcctcaac ctgatgacgt cggcgtggtt cgcggccggc ggtgtgctgt
      961 cgagcttcat caacagcctg ccgaagcgca aggacaagcc cgcggcgttg ccgagcgaca
     1021 tgagcacgac gtcgctggtc ggcggcggct tgatagcagg cgacgcactg gcggcgctgg
     1081 gcttgggcat cgcgggactg ctcggcactt tgttcggctg acgccagcgt ctcgacggcg
     1141 ccgacggccg ccgcaactgg tattgccctg aatcaatcga tggggccccg cggccccaag
     1201 gcggggggcg tctgctagct gatcaaaggc gttggaccgg tggatcaaag ccatagacca
     1261 gtataatacc aattgactaa ctggtatttc cggtacaagc tagctggatt atccgttcgc
     1321 aggtgttgaa tccattgaac cccgcccgat cgacggcgat ccgcttccac atcggcatcg
     1381 acggcggggg cacgcatacg cgcgcgcggc tcgcgtccac cgaccgcgcg gtgctgggct
     1441 tcgccgcgtc gggaccgtcc gcgctcggcc agggaatcga gcaggcatgg cagcacatcg
     1501 tgcaggccgt cgacggcgcg tttgccgacg cggggctcgc ccccgcaccg cgcgagcaat
     1561 gcgcgctggg tctggggctc gccggcgcca acgtgcggca acgttgcgaa gaattcttcc
     1621 gccgcgcgcc gccgttcgct gagatcgcac tggacactga cgcgtatacg accttgctcg
     1681 gtgctcacga aggacgaccc ggggcgatcg tcgcggccgg taccggcagc gtcggtgaag
     1741 cgttgcgccg cgatggtctg cgtgtggcgg tcggcgggtg gggatttccc gtcggcgacg
     1801 aaggcagcgg cgcgtggctc ggattgcgcg cgatgcgcga gacgcatcgc gcgatcgacg
     1861 gccgcgccca cgtcggccca ctggtcgggg ccgttcttgc ggtggcgggc acatcgcgcg
     1921 aggcgttgtt gggctggtgc gatcgaccgg ggcagaacgc ctatgcggcg ctcgccccgc
     1981 tggtgttcga cgcggccgcg accgatccgt ttgccgccgc cctgctagat cgcgcggcgc
     2041 gctcgctcga ggaaatagcg gccgcgctcg accccgagag agtgctgccg ctggcaattt
     2101 tcggcagcat cgggcaaagg ctgctgcctc gtttcgaacc accgatccag gcgcggtgcg
     2161 tcgagcccgc aggggactcc gccgacggcg cgcttcgcct cgtcggccag gcgctcagtg
     2221 agctgggcgg aagcgagcga tgaaaggtcg cccgttcgag tttcgcgtag acgcaagagc
     2281 ggcctcgccc ctatacatgc agctcgcgca aaagcttgcg caggcgatac gcaacgatga
     2341 ctaccagccc aacgaagcgt tgccgtcaga gcgggtgctg tcggaatcgc tcagcttgtc
     2401 gcgcgtgacg gcgcgcaagg cgatcgatca gctggtcgag cagggactgg tcgtgcgtcg
     2461 gcgcggctcg ggcaactaca tagcacctcg ggtcgaacag ccgctgtcgc gcctgaccag
     2521 cttttccgag gagctgcacc ggcgcggctt cacgccttcg tcgcgctggc tcgcacgcgg
     2581 ctgcaccgca gcggcgcccg acgagcagct ctcgctgggc ttgtcgccag gctcgcgcgt
     2641 cgcgcgcctc gagcggttgc gcctggccga caacgtggtc atggcctacg aagtcagcgt
     2701 gcttcccgag caggttctgc cggatccttc gcaggtcgat gcatcgctgt acgcgcatct
     2761 ggcgatgctg ggccgcgcac cggtgcgcgc gctgcagcat cttcgcgcgc tcaacgccga
     2821 cgcgcggctc gcgcaattgc tcgaagtgcc gaccggacgc gcggtcctgt tcatcacccg
     2881 ggttggctac ctggacaccg ggcgggcagt cgaactcacg cattcgtatt gccgcagcga
     2941 ctattacgat ttcgtcgccg agaccaggcg ccaagcgtga gcgcgttgcg catcgaagga
     3001 cacatcctga cgccgcaagg cttcgtgcgc ggcgtgctcg agcacgaaga aggccgcatc
     3061 acgcgcgtcg aaggcacgct gcggaccgag gaccaggcga ggagcgaagc gcaagggctg
     3121 gtgctgcccg gattcatcga tacgcacgtg cacggcggcg gcggcagcga cacgatggaa
     3181 ggcggcgagg cgattggccg catcgcccgg ttgcatgcac ggcatgggac aacgtcgctg
     3241 ctggccacga cgatgacggc gccgcttgcc gaagtggaag cggcgatgcg agcgctggct
     3301 tctgcctgca gccaccgcgc tagcggtgca gcgcgcgtgc tgggcgtgca cctggaagg
//
LOCUS       DATANS010000713         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_498229, whole genome shotgun sequence.
ACCESSION   DATANS010000713 DATANS010000000
VERSION     DATANS010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498229"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..2031
                     /locus_tag="VHT22_10655"
     CDS             340..2031
                     /locus_tag="VHT22_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013635043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HEX3138061.1"
                     /translation="MYFRRKTSAGRAYLQIVESRRDGDQVRQQVIATLGRFDELQASG
                     QLERLVRSGARFAAKAMVLSAASNDAAIKIAVRRIGPALVFERLWEETGCRAVIADLA
                     SKRGHKFALERAAFLTVLHRLFVSGSDRAADRWREDYAIAGVEGLDLHHLYRAMAWLG
                     EELAEKEQDGRTPFAPRCLKDVVEERLFAHRRDLLTRLDLVFMDTTSLYFEGAGGQTL
                     GQHGYSKDHRPDLRQMILAVLIDGDGRPVCSEMWPGNTADVTTLIPVIERLRRRFDIA
                     RVCVVADRGMISAATMAELEARRLLYILGVRERSDKLVRELVLDDPAPFVPLLMKKRS
                     KEIEYGAKTVLLAGRRYIVCRNHQEAAKDAADRASIVAALERQLAKGDKALVGNTGFR
                     RYLKTISEEHFAIDPDKIEEERKLDGIFVLRTNTDLNPLEAMLCYKQLWTVEQTFRTA
                     KHLLSTRPFHKLDETIRGHVFCSFLALVLKTELEQRIVGLGRVRSSWPEIVADLDSLT
                     ETEIEYDGKRFVVRAAPRHAASLALRAAGVALPPTCVTPQSTDYHLPRRRSATLP"
BASE COUNT          356 a          634 c          700 g          375 t
ORIGIN      
        1 cctcgtgcgg cgccgtgacg taggagccgg cgaccgccat cgcggcaagc ccgatcacca
       61 ggatcgcgcc gaagaccaca ttgaccttga gccaacggtc gaacgtgcgc cggtcgtcgg
      121 gcgacagcat cttgtagtgg cgtgggatat tcgatcgatc catgactcat cttctacagt
      181 tcgcgcaggt ggacgcggca taagtcattg attttgcttg gtgtgcggtg gtgttttcgt
      241 aaatatgtag tgccacgttt ttgttcgatt taatttgatt ttttcaatcg aatcatagct
      301 ccgtgtgcgc accagaacgc ggaataagaa gccgccgcca tgtatttccg ccgcaagacc
      361 tccgccgggc gcgcctatct gcagatcgtc gaaagccgcc gcgacggcga tcaggtgcgc
      421 cagcaagtga tcgcgacgct tggacgtttc gatgagctgc aggccagcgg ccagctggag
      481 cggctggtac gctcgggtgc gcgctttgca gccaaggcca tggtgctgag cgcggccagc
      541 aatgatgctg cgatcaagat cgcagtgcgt cggatcgggc cggcgctggt gttcgagcgg
      601 ctgtgggagg agaccggatg ccgcgcggtg atcgccgatc tcgccagcaa gcgcgggcac
      661 aagttcgcgc tggagcgcgc ggcgttcctc accgtgctgc accggctgtt cgtgagcggc
      721 tccgaccgcg ccgccgatcg ctggcgcgag gattacgcca tcgccggcgt cgaggggctc
      781 gatctgcatc acctctatcg ggcgatggcg tggctgggcg aggagctggc ggagaaggag
      841 caggacggcc gcacgccgtt tgccccgcgc tgcctgaagg acgtggtgga ggaacgactg
      901 ttcgcgcatc ggcgcgatct gctcacgcgg ctcgacctgg tgttcatgga caccaccagc
      961 ctgtacttcg agggcgccgg tggccagacg ctgggccagc acggctacag caaggaccat
     1021 cggccggatc tgcggcagat gatcctcgcc gtgcttattg atggcgatgg gcggccggtg
     1081 tgctcagaga tgtggccggg caacaccgcc gatgtgacga cgctgatccc ggtgatcgag
     1141 cggctgcgcc ggcgcttcga catcgcacgg gtgtgcgtgg tcgccgaccg cggcatgatc
     1201 agcgccgcga ccatggccga gctggaggca cggcggctgc tgtatatcct gggggtgcgc
     1261 gagcgctccg acaagctggt gcgcgagctg gtgctcgacg atccagcgcc gttcgtgccg
     1321 ctgctgatga agaagcgcag caaggagatc gagtacgggg ccaagacggt gttgctggcg
     1381 gggcggcgct acatcgtctg tcgcaaccac caggaggcgg cgaaagacgc cgccgaccgc
     1441 gcctcgatcg tggccgcgct ggagcgccag ctcgccaagg gcgacaaggc gctggtcggc
     1501 aacaccggtt ttcggcggta tctcaagacc atcagcgagg agcattttgc gattgatccc
     1561 gacaagatcg aggaggagcg caagctcgac ggcatcttcg tgctgcgcac caacaccgat
     1621 ctcaatccgc tcgaggccat gctctgctac aagcagctgt ggacggtgga gcagaccttc
     1681 cggacagcaa agcatctgct ctcgacgcgg ccattccaca agctcgacga gaccatccgc
     1741 ggtcacgtgt tctgcagctt tctggcactg gtgctgaaga ccgagttgga gcagcggatt
     1801 gtcggcctcg gtcgcgtgag gagctcctgg ccggagatcg tcgccgatct ggattcgctg
     1861 accgagaccg agatcgagta cgacggcaaa cgcttcgtcg tccgcgcggc gccccgccat
     1921 gccgccagct tggccctgcg ggccgccggc gtcgccctgc cgccgacctg cgtgacgccg
     1981 cagtccactg attaccatct tcctcgcagg cgtagtgcca cgctgccgta gcagcgcgga
     2041 ttgctattgc cactcagcta tttag
//
LOCUS       DATANS010000714         2974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_161710, whole genome shotgun sequence.
ACCESSION   DATANS010000714 DATANS010000000
VERSION     DATANS010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161710"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VHT22_10660"
     CDS             <1..355
                     /locus_tag="VHT22_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138062.1"
                     /translation="IARLGEDAPLSVASYCAAYRACGNRSEREWQIALIVEVGAALDR
                     YVHMPLLRSALAAMRRPARIAGVGALQEFLERGLLSFRGMKGAGEFLAVIKTRETAVM
                     QAILAGDETPFAEPA"
     gene            complement(364..648)
                     /locus_tag="VHT22_10665"
     CDS             complement(364..648)
                     /locus_tag="VHT22_10665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEX3138063.1"
                     /translation="MPSIAIKRRHKLEPKKAKAAAQKVIKDLSARYQLACSWDGDQVE
                     FERPGVSGHMHLRKNEVLLDVQLSFLMTPLKGPIERAINEELDRLFGDAA"
     gene            815..1555
                     /locus_tag="VHT22_10670"
     CDS             815..1555
                     /locus_tag="VHT22_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3138064.1"
                     /translation="MKQTQTESRAIGAAQPRGPAPTKNRILDAAEALFMEHGFEATSL
                     RQITALAEVNLAAVSYHFGSKEELFESVLTRRLDPMNRDRIALLTRFEREAAPDVLSC
                     ERILTAMFIPALELARDRQRGGQNFLRLLGRAYADPAPFIRRFLSEQYAVMIARFKTA
                     FGRALPELPKKELSWRLHFVMGALSYTLAGTDALKLIAALSPREAGNDEMLLHRLAPF
                     LVAGLKAPLPDLSELDGGSVPMRQAGGR"
     gene            1555..>2974
                     /locus_tag="VHT22_10675"
     CDS             1555..>2974
                     /locus_tag="VHT22_10675"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX3138065.1"
                     /translation="MLTLLWLVAVVIGFVALAYVNAAGWLWILGIGAALIAALALHAL
                     PALLIGILAVLLFLLAIPLNWPWLRRMLVSDGVLTAFRKALPPMSPTERDAIEAGTVW
                     WDGELFSGRPAWQKLLALPPPRLSDQEQHFLDHDCEQLCAMISDWETTHVYRDLSPEA
                     WAFVKERGFLGMIIPKAYGGLGFSAYAHSQVITKLSTHASAVAVTVMVPNSLGPGELL
                     LHYGTDEQKNHYLPRLAKGIEIPCFALTNPHAGSDAAAIPDRGIVCIGQHEGKATLGL
                     RVTWDKRYITLGPVATLLGLAFRAYDPEHLLGGTEDLGITCALIPTRHPGVNIGRRHM
                     PLNAVFQNGPNWGKDVFIPIDWVIGGRAQVGNGWRMLMESLAAGRGISLPSSSTGMAK
                     LAVRATGAYARVRTQFKTPIGKFEGVEEALTRMGGNLYMMDATRMLTALAVDLGEKPA
                     VLSAIAKYHLTERARDVVIDGMD"
BASE COUNT          486 a          955 c          998 g          535 t
ORIGIN      
        1 tatcgctagg ctcggcgagg atgccccgct atcggtggct tcgtattgcg ccgcgtaccg
       61 tgcgtgcggc aaccgcagcg aacgcgagtg gcagatcgcg ctgatcgtcg aggtcggcgc
      121 cgcgctcgat cgctacgtgc atatgccgct gctgcgttcg gcgcttgccg cgatgcggcg
      181 gcccgcgcgc atcgccggtg tcggcgccct gcaggaattt ctcgaacgcg gcctgctgtc
      241 gttccgcggc atgaaaggtg cgggcgaatt cctggcggtg atcaagacgc gcgagaccgc
      301 agtgatgcag gcgatcctcg ccggcgacga aacgccgttc gcggagccgg cctgacgcga
      361 ccttcaggcc gcatcgccga acaggcggtc gagctcctcg ttgatcgccc gctcgatcgg
      421 gcccttgagc ggcgtcatca ggaacgagag ctgcacgtcg aggaggacct cgttcttgcg
      481 caggtgcatg tggcccgaca cacccgggcg ctcgaactct acttgatcgc cgtcccacga
      541 gcaggcaagc tgatagcgcg cactcaagtc cttgatgact ttttgcgcgg cggccttcgc
      601 cttcttcggc tcgagcttat ggcggcgttt gatggcgatc gagggcatcg aaaagatccg
      661 gtaccttgaa ccgcgagtat agccaaacgg cgcccgacgg cccgggccgc gagccgccat
      721 tgacagcatc cacggcaggc cggtagcatt caaacaaacg tttaactgaa ttgcccgcga
      781 gcgggttccc gcccgccgga cgcccggaga tccagtgaag caaacgcaga ccgaatcgcg
      841 cgcgatcggc gcggcgcagc cacgcgggcc ggcgccgacc aagaacagaa ttctggacgc
      901 tgccgaagcc ttgttcatgg aacacggttt cgaggcgacg tcgctgcgcc agatcacggc
      961 gctggcggag gtcaacctcg ccgccgtcag ctatcacttc ggcagcaagg aagagttgtt
     1021 tgaatccgtg ctgacgcgac gcctcgatcc gatgaatcgc gatcgcatcg cacttttgac
     1081 gcgtttcgag cgcgaggcag cgccggacgt gctgtcgtgc gagcgcatcc tgacggcgat
     1141 gttcatcccc gcgctcgagc tcgcacgcga tcgacagcgc ggcgggcaga atttcctgcg
     1201 cttgctcggt cgtgcctatg ctgatccggc gcccttcatc cggcgattcc tgtccgagca
     1261 gtacgcggtg atgatcgccc gcttcaagac ggcgttcggg cgtgcgttgc cagagcttcc
     1321 gaaaaaggag ctgtcgtggc gcctgcactt cgtcatgggc gccctgtcgt acacgcttgc
     1381 gggaaccgac gcgctgaaac tgatcgcggc gctcagtccg cgcgaggcgg gcaacgacga
     1441 aatgctgctg catcgcctgg cgccgttcct ggtcgcgggg ctgaaggcgc cgctgcccga
     1501 cctgtccgag ctcgacggtg gcagtgtccc gatgcggcaa gcgggaggtc ggtaatgctg
     1561 actcttctgt ggctggtcgc agtcgtcatc ggctttgttg cgctcgccta cgtgaacgcc
     1621 gccgggtggc tgtggattct gggcatcggc gcggcactga tcgcggcgct cgcgctgcat
     1681 gcgctgccgg cgctgctgat cggcatcctg gcggtgctgc tgttcctgct ggcgattccg
     1741 ttgaactggc cgtggctgcg ccgaatgctc gtcagcgacg gcgtgctgac agcgtttcgc
     1801 aaggcactgc cgccgatgtc gcccaccgag cgcgatgcga tcgaggccgg cactgtctgg
     1861 tgggatggcg agctgttctc cggtcgcccg gcgtggcaaa agctgcttgc gctgccgccg
     1921 ccgcgcctca gcgaccagga gcagcatttt ctcgaccatg actgcgaaca gctgtgcgcg
     1981 atgattagcg actgggagac gacgcacgtc taccgcgacc tgtcgccgga ggcgtgggcg
     2041 ttcgtcaagg agcgcggctt tctcgggatg atcattccga aggcctacgg tggactcggc
     2101 ttctcggcgt atgcgcactc gcaggtgatc accaagctct cgacgcatgc cagcgccgtg
     2161 gcggtgacgg tgatggtccc caattcgctc ggcccgggcg agctgttgct gcactacggc
     2221 accgacgagc agaagaacca ctacctgccg aggctcgcca agggaatcga gatcccgtgc
     2281 tttgcgttga ccaatccgca tgcgggctcc gatgcggcgg cgattcccga tcgtggcatc
     2341 gtctgcatcg ggcagcacga gggtaaagcg actctcggcc tgcgcgtcac ctgggacaag
     2401 cgttacatca cgctggggcc ggtcgcaacc ttgctgggcc tcgcgttccg cgcctacgac
     2461 cccgaacatc tgctgggcgg caccgaggat ctcggcatca cctgcgcgct gatcccgacc
     2521 cggcatccgg gcgtcaacat cggccgccgc cacatgccgc tgaacgcggt gttccagaac
     2581 ggaccgaact ggggcaagga cgttttcatt ccgatcgact gggtgatcgg cggacgagcg
     2641 caggtcggca acggctggcg gatgctgatg gaatcgctgg ccgcgggccg cggcatctcg
     2701 ctgccttcgt cgtcgaccgg catggccaag ctggccgtgc gtgcgaccgg cgcgtatgcg
     2761 cgtgtccgca ctcagttcaa gacgccgata ggcaagttcg aaggcgtcga agaagcgctg
     2821 acgcgcatgg gcggcaatct gtacatgatg gacgcaacac ggatgctcac cgcgctggcg
     2881 gtcgacctgg gtgagaagcc cgccgtgctg tcggccattg ccaaatacca tctgaccgag
     2941 cgcgcgcgag acgtggtcat cgacggcatg gatg
//
LOCUS       DATANS010000715         1594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_243635, whole genome shotgun sequence.
ACCESSION   DATANS010000715 DATANS010000000
VERSION     DATANS010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243635"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VHT22_10680"
     CDS             <1..786
                     /locus_tag="VHT22_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3138066.1"
                     /translation="PIGAGIACLFGVLLGAPTLKLRGDYLAIVTLGFGEIIRIFLNNL
                     NAPINFTNGPQGINLIDPFRIGGFSFASTESFLGITFTGPQKYYYMLLLLTIAVIVVN
                     LRLQNSRLGRAWEAIREDEVAAKAMGINTRNVKLLAFAMGASFGGIAGAVFSATQQFI
                     SPESFGLFESIIVLAMVVLGGMGNIPGVILGAILLTLLPEVLRYTMEPLQMGLFGRVL
                     IDAETIRLLVFGVALVAIMLFRPAGLWPSAVRKRELEVRKAAA"
     gene            812..>1594
                     /locus_tag="VHT22_10685"
     CDS             812..>1594
                     /locus_tag="VHT22_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3138067.1"
                     /translation="MSERTLLSAEDIGKRFGGVQALADVSFSIRHGEIYGLIGPNGAG
                     KTSLFNVLTGIYEPDGGSFTFDGAPLNRLKPNEVAERGIARTFQNIRLFQNLSALENV
                     MIGRHVRTRAGVFGAIFRDRATLAEEAAIASRAYQLLDYVGVATRANDLAKHLAYGDQ
                     RRLEIARALATDPKLLALDEPAAGMNATETRGLKGLLEDIRRDGTTILLIEHDMKLVM
                     SVCDRVLVLDYGRKIAEGSAADVQNDPKVIAAYLGGEVTLHVR"
BASE COUNT          271 a          520 c          518 g          285 t
ORIGIN      
        1 ccgatcggcg cggggatcgc ctgcctgttc ggcgtgctgc tcggcgctcc gaccttgaag
       61 ctgcgcggcg actacctggc gatcgtcacg ctcggcttcg gcgagatcat ccggatcttc
      121 ctcaataacc tgaatgcgcc gatcaacttc accaacgggc cgcaagggat caacctgatc
      181 gatccgttcc gcatcggtgg gttctcattc gccagcaccg agtctttcct cggcatcacc
      241 ttcaccggcc cgcagaagta ctactacatg ctgctgctgc tgacgatcgc ggtcatcgtc
      301 gtcaatctgc ggctgcagaa ctcgcggcta ggccgggcgt gggaggcgat acgcgaagac
      361 gaggtcgccg ccaaggcgat gggcatcaac acgcgcaatg tcaagcttct cgcgttcgcg
      421 atgggcgcct cgttcggcgg catcgccggc gccgtcttct cggcgacaca acagttcatc
      481 agtccggaaa gcttcggcct gttcgagtcg atcatcgtgc tcgcgatggt ggtcctgggc
      541 ggcatgggca acattcccgg cgtgatcctt ggcgcgatcc ttctgaccct gctgcccgag
      601 gtattgcgct acacgatgga gccgctgcag atggggctgt tcggccgcgt gctgatcgac
      661 gcggagacga tccgcctgct ggtgttcggc gttgccctgg tcgcgatcat gcttttccgg
      721 ccggccggac tgtggccgtc tgcggtgcgg aagcgcgagc tcgaagtccg gaaggcagcg
      781 gcctagaccg gcgcaacgga agattcggcc cgtgagcgag cgaacgctgc tttccgccga
      841 ggatatcggc aagcgcttcg gcggcgtcca ggcgctggcc gatgtcagct tcagcatccg
      901 tcacggagag atctacggcc tgatcggacc gaacggcgcg ggcaagacct ctctgttcaa
      961 cgtgctgacc ggcatctacg agccggacgg cggcagcttc actttcgacg gtgcgccgct
     1021 caatcgcctg aagccgaacg aagtcgccga gcgcgggatc gcgcgaacct tccagaacat
     1081 ccgcctgttc cagaacctgt cggcgctgga gaacgtgatg ataggccgcc atgtgcgcac
     1141 ccgcgccggc gtcttcggcg cgattttccg cgaccgcgcc acactcgccg aggaagcggc
     1201 gatcgccagc cgcgcctacc agttgctcga ttatgtcggc gttgccacgc gggccaacga
     1261 cctggccaag catctggcct acggcgacca gcgccggctg gagatcgcgc gcgcactggc
     1321 gaccgacccg aagctccttg cgctcgacga gcctgccgcc ggcatgaatg cgaccgaaac
     1381 tcgggggctc aagggtctgc tcgaggacat ccggcgcgac ggcacgacga ttctcctgat
     1441 cgagcacgat atgaagctgg tgatgagcgt ctgcgaccgc gtgctggtcc tcgactatgg
     1501 acgcaagatt gccgagggaa gcgccgcgga cgtgcagaac gatccgaagg tgatcgccgc
     1561 ctacctgggc ggcgaggtca cgctgcacgt cagg
//
LOCUS       DATANS010000716         2409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_457093, whole genome shotgun sequence.
ACCESSION   DATANS010000716 DATANS010000000
VERSION     DATANS010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457093"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VHT22_10690"
     CDS             complement(<1..1082)
                     /locus_tag="VHT22_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3138068.1"
                     /translation="MQPTSRQLGRCAIGLALLGIACAAIAQTVLDSPELYPGEKALYE
                     AARKEGMVVSFDTGPTWANWAAEFSAFKKRYPEVEIVYNDIGSAATVVALDKARNRPQ
                     ADTAYYFAGSAFDAVSKNVVAPFKPVNFDKLTPVFRDQDGKWFTIHTLTIAFVVNTKL
                     VKNVPQSWADLLKPEYKNSIVYLDPRSTGVGQVGVLAANFGNGGDMSNLQPGFEYFRK
                     LQQSGNVLRVLGTTPYAQFVKGEIPIWISYENDGLKAKYTDGLGDAVAVVIPKEASAA
                     APYAISLVANGPNPDAGKLWLNFIMTDTGQGIFAQGFVRPSVPGVKLPDNVADKLPAA
                     PQVRPLDVKSAAAKKSDIDREWAAATG"
     gene            complement(1097..2134)
                     /locus_tag="VHT22_10695"
     CDS             complement(1097..2134)
                     /locus_tag="VHT22_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3138069.1"
                     /translation="MSPLAVSLEGVTFRYPGTNAGVFDLTMTIAPGELVVCLGPSGCG
                     KTTLLRLVAGFLKPDAGVIRLNGSDVSSLPARARECGVVFQAYALFPHMRVWENVAYP
                     LRVRDIALDERRRLAEQMLHLVGLEGLAERLPAQLSGGQQQRVALARALVFKPRALLL
                     DEPLSALDAATRSTMRDEVRRIQRAQNIATLLITHDQDEALSLADRMVLLREGRLVQS
                     GSPHEIYDRPADAFVAGFVGRANLLDGRVIDAETVDTAIGRLATPRHGHAAGSALRLL
                     VRPERVEIGAAASGENVFAAQVLRDRFFGATRQLEVAAGDGRLEIETSMRDHVARVRV
                     PREAIQFLNAH"
     gene            complement(2131..>2409)
                     /locus_tag="VHT22_10700"
     CDS             complement(2131..>2409)
                     /locus_tag="VHT22_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011548936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3138070.1"
                     /translation="QRFVHVVLPALRHSILAGLIIVAALSIGEFQFSNLVAGFLNRTY
                     PIVLLQAFYGATGFACAATVILLALALAAAIGGGLTAGGATRLKTAAA"
BASE COUNT          427 a          791 c          779 g          412 t
ORIGIN      
        1 ccggttgccg ccgcccactc gcggtcgatg tcggactttt tcgccgccgc cgacttgacg
       61 tcgagcggac gaacctgcgg cgcggcgggc agcttatcgg cgacgttgtc ggggagcttg
      121 acgccgggca ccgacggccg cacgaacccc tgcgcgaaga ttccctgtcc tgtgtcggtc
      181 atgatgaaat tgagccagag cttgcccgcg tccgggttgg gaccgttggc gaccaggctg
      241 atcgcgtacg gcgccgccgc ggaagcttcc ttggggatca cgaccgccac cgcgtcgccc
      301 aggccgtcgg tgtactttgc cttcaacccg tcattttcgt acgagatcca gatcggaatc
      361 tcgcctttga cgaactgtgc gtacggcgtc gtgccgagta cgcgcagcac gttgccggat
      421 tgctgcagct tgcggaagta ttcgaagccc ggctgcagat tgctcatgtc gccgccattg
      481 ccgaagttcg ccgcaagcac acccacctgg ccgacaccgg tcgagcgcgg atcaaggtag
      541 acgatgctgt tcttgtattc cggcttgagc aggtcggccc acgactgcgg cacgttcttg
      601 accagcttgg tattgacgac gaaagcgata gtcaaggtgt ggatcgtgaa ccatttgcca
      661 tcctgatcgc ggaataccgg cgtcagctta tcgaaattga ccggcttgaa cggcgcgacg
      721 acgttcttgc tgacggcgtc gaacgccgag ccggcaaaat agtaggcagt gtcggcctgc
      781 ggacggttgc gcgctttgtc cagcgcgacg acggtggccg ccgatccgat gtcgttgtag
      841 acaatctcga cctccggata ccgcttcttg aatgcggaga attcggccgc ccagttggcc
      901 caggtcggac cggtatcgaa gctcacgacc atgccttctt ttcgcgccgc ttcgtaaagc
      961 gctttttctc ccggatagag ctcgggcgag tcgagcacgg tttgcgcgat cgccgcgcag
     1021 gcgatcccca gcagggcgag gccgatcgcg cagcggccca gttggcgtga tgtcggttgc
     1081 atggtcttct cctccttcag tgggcattca ggaactggat ggcctcgcgc ggcacgcgca
     1141 ccctcgcgac atgatcgcgc atcgaggttt cgatttcgag tcggccgtcg ccggcggcga
     1201 cctcgagctg ccgggtggcg ccaaaaaaac ggtcgcgcaa cacctgcgcg gcaaagacat
     1261 tctctccgct cgccgcggcg ccgatttcga cccgctccgg gcgcaccagc aagcgcagcg
     1321 cgctgccggc ggcatgtcca tgccttggcg tcgcaaggcg gccgatcgct gtatcgacag
     1381 tttcggcgtc gatgaccctg ccatcgagca gattggctcg cccgacgaag ccggcaacga
     1441 aagcatcggc gggccgatcg tagatttcgt gcggcgaccc cgactggacg aggcggccct
     1501 cgcgcagcag gaccatgcga tcggccaacg acagcgcttc atcctggtcg tgcgtgatca
     1561 gcagcgtcgc gatgttctgc gctcgctgaa tgcggcgcac ttcgtcccgc atcgtgctgc
     1621 gcgtggcggc atccaatgcc gacagcggct cgtcgagcag cagggcgcgc ggcttgaaga
     1681 ccagcgcgcg cgccagcgcc acacgctgct gctgcccgcc ggaaagctgt gccggcaggc
     1741 gctcggcaag tccctccagc ccgaccaggt gcagcatctg ttcggcaagg cggcgccgct
     1801 cgtccagcgc gatgtcgcgc acgcgcaacg gataggcgac gttctcccac acgcgcatgt
     1861 gcggaaacag cgcatacgcc tggaacacca cgccgcattc gcgcgcccgc gccggcaggc
     1921 tgctgacatc gctgccgttc agccggatga cgcccgcatc gggcttgagg aagccggcga
     1981 cgagccgcaa caacgtcgtc ttgccgcagc cgctcggccc gaggcaaacg accagttcac
     2041 ccggggcgat cgtcatagtc aggtcgaaca ctccggcgtt ggtgcccgga taacggaaag
     2101 tgactccctc gagcgatacg gcgagcgggc tcatgccgca gcggttttca accgcgtcgc
     2161 gccgccggcg gtgagaccgc caccgatcgc cgcggcgagc gcgagcgcaa gaaggattac
     2221 tgtagcggcg caggcaaatc cggtcgcgcc atagaacgcc tgcaacagca cgatcggata
     2281 ggtgcgattc aggaagcccg ccaccaggtt ggagaactgg aactccccga tcgacagcgc
     2341 cgcgacaatg atcaggccgg cgagaattga atggcgcaaa gccggcagga cgacgtgcac
     2401 gaagcgctg
//
LOCUS       DATANS010000717         2602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_331181, whole genome shotgun sequence.
ACCESSION   DATANS010000717 DATANS010000000
VERSION     DATANS010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331181"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VHT22_10705"
     CDS             <1..212
                     /locus_tag="VHT22_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015872148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3138071.1"
                     /translation="VERVFAKFHRGAVEGAGTGFGLGLAICRAIVRLHGGRAWAESVP
                     AGGTAFHFSLPLEEAPSVPPEPASS"
     gene            215..910
                     /locus_tag="VHT22_10710"
     CDS             215..910
                     /locus_tag="VHT22_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006891407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3138072.1"
                     /translation="MTDAHPVILIVEDEPEIRRFLRAAFGVEGYRVVESATGRRGSID
                     AGTHKPDLAIVDLALPDIDGIEVIRRIREWSPMPIIVLSARVQEQSKIDALDAGADDY
                     VTKPFGVGELLARVRAALRNAVRSQSGASVLRFDSAEIDLERRTASRDGEKIRLTPIE
                     FRMLTTLAKHLGMVVTHRQLLTEVWGPTHESDTHYLRIYMKQLRQKLESDPVQPRHLL
                     TETGVGYRLVIDD"
     gene            996..1874
                     /locus_tag="VHT22_10715"
     CDS             996..1874
                     /locus_tag="VHT22_10715"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3138073.1"
                     /translation="MPEARPARGPTITAIDCHAHVMRRDAPLAPDRHSVPKRDCTVEE
                     YLDVLDANGISQGVLTAPSFYGADNSLLLDALDRAAGRLRGTVIVDPGIARASLAAMG
                     ARGAVGIRLNWFRRSALPDVSDTGYRQLFANVRDLGWHVEIYLEGPLLAKVLPAIRDS
                     GATVVVDHFGSPDPARGVACAGFQAVLDGVGTGDTFVKLSAPYRQGGADPQRYVDALL
                     DAGGAEQLVWASDWPFVSHEDEISYRDCVRWLAGWVPDDGTRRIILADTPARLFRFDR
                     LLPAAADKQKSEEASP"
     gene            1871..>2602
                     /locus_tag="VHT22_10720"
     CDS             1871..>2602
                     /locus_tag="VHT22_10720"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX3138074.1"
                     /translation="MKLVRIVVGMLLLAATSAAAAQVIKLIVPTAPGGGTDGYFRILA
                     KEATPFIKDTIVIINVPGAGGTIGVAQMVHSPPDGQTLAAVWLGPVTVSPNTMRVPYT
                     PNDYIPVIQLTSAPYVFCVHPDFAATEGKSFIEELRKNPDKYTYGDDGIGGPGQLAAE
                     RIFRAMKVKARDIPFKGAGETLTAFLGKHIDIYVGSVPPILQHAQSGNANCVLLSSAD
                     RIASLPAASSLRDLGIPDEELILWRG"
BASE COUNT          438 a          867 c          848 g          449 t
ORIGIN      
        1 acgtcgagcg cgtattcgcc aagtttcatc gcggcgcggt cgaaggcgcc ggcaccggct
       61 tcggtctggg gctcgcgatc tgccgcgcga tcgtccggtt gcacggtgga cgggcctggg
      121 cggagagcgt gcccgccggc ggaacggcat ttcatttctc gcttcctctg gaagaagcgc
      181 catcggttcc gcccgagccg gcatcgagct gaccgtgacg gacgcgcatc cggtcattct
      241 catcgtcgag gacgagcccg agatccgtcg gtttctgcgc gccgctttcg gcgtcgaagg
      301 ttaccgtgtc gtcgaatcgg ccaccggccg ccgcggctcg atcgacgccg gaacccacaa
      361 gcccgatctc gccatcgtcg atcttgcgct tcccgacatc gacggcatcg aagtgatccg
      421 acgaatccgg gaatggtcgc cgatgcccat catcgtgctg tcggcgcgcg tgcaggagca
      481 atccaagatc gatgcgctcg acgcaggtgc cgatgactac gtcaccaagc ccttcggcgt
      541 cggcgagctg ctggcgcgcg tccgcgccgc gctacgcaac gccgtgcgct cacaatcggg
      601 ggcgtcggta ttgcgtttcg acagcgctga aatcgatctc gaaaggcgca ccgcgagccg
      661 cgacggcgag aagatccggc tgactccgat cgaattcagg atgctcacga cgctggcgaa
      721 gcatctggga atggtcgtca cacaccgcca gctgctcacc gaagtgtggg ggccgacgca
      781 cgagagcgac acgcattacc tgcgcattta catgaagcaa ttgcgccaga agctggagtc
      841 agatcccgtg caaccccgcc atttgctcac ggagaccggc gtcggatatc gcctggtcat
      901 cgacgactag gcgaggtgcc gccgcggcga ttgctcgcga agtcacttca gccgtcgtcg
      961 gttgcgcgcg ttcgggcatc cgctaataat ggcgcatgcc tgaagctcgt ccggcgcgag
     1021 gcccgacaat caccgccatc gactgccacg cgcatgtgat gcggcgagat gcgccgctgg
     1081 cgccggaccg gcactcggtg ccgaagcgcg attgcacggt cgaggaatac ctggacgtgc
     1141 tggacgcgaa cggcatctcg cagggcgtac ttaccgcgcc gagcttctac ggcgccgaca
     1201 attcgctgct gctggacgcg ctggatcgcg cggcgggacg gctgcgcggg accgtcatcg
     1261 tcgatccggg catcgcccgc gcatcgctcg cggcaatggg cgctcgcggc gcggtcggca
     1321 tccggctcaa ctggttccgc cgttccgcat tgcccgatgt ttccgacacc gggtaccggc
     1381 agcttttcgc gaacgtgcgc gatctcggct ggcatgtcga gatctatctg gaagggccat
     1441 tgttggcgaa ggttctgccg gcgatccgcg acagcggcgc cacagtcgtc gtcgatcatt
     1501 tcggcagccc cgatccggcg cggggcgtcg cgtgcgccgg cttccaggcg gtgctcgatg
     1561 gcgtgggcac cggcgatacg ttcgtcaagc tctcggcgcc gtatcgccag ggcggcgccg
     1621 atccacagcg ctatgtcgac gcactgctag acgccggcgg cgcggaacag ctggtctggg
     1681 ccagcgactg gccgttcgtc tcgcacgagg acgagatcag ttatcgcgac tgcgtgcgat
     1741 ggctcgccgg ttgggtgccc gacgacggga cgcgccggat catcctcgcc gatacgccgg
     1801 cccgcttgtt ccgcttcgac cggctgctac cggcagcagc ggataaacaa aaatcggagg
     1861 aagcctcgcc atgaaacttg ttcgcatcgt cgtcggcatg ctgctgctag ccgcgacttc
     1921 ggccgcggcc gcccaggtga tcaagctgat cgttcccacc gcgccgggcg gcggtaccga
     1981 cggttacttc cgcatcctgg ccaaggaggc gacgccgttc atcaaggata cgatcgtcat
     2041 catcaacgtc cccggcgccg gcggaacgat cggcgtcgcg cagatggtgc attcgccgcc
     2101 cgacggacag acgctcgccg ccgtgtggct gggtccggtc accgtgtcgc cgaacacgat
     2161 gagggtgcca tacacgccga acgactacat tccggtcata cagctgacca gcgcgccgta
     2221 cgtcttctgc gtgcatccgg atttcgccgc gaccgaaggc aagagcttca tcgaggagct
     2281 gcgcaagaac ccggacaagt acacctatgg cgacgacggc atcggcggcc ccggccagct
     2341 cgcggccgag cgcatctttc gtgcgatgaa ggtcaaggcg cgcgacattc cgttcaaggg
     2401 cgcgggcgaa acgctgaccg ctttcctcgg caagcacatc gacatctacg tcggatcggt
     2461 gccgcctata ctgcagcacg cccaatctgg caatgcgaac tgcgtgctcc tgagctccgc
     2521 cgaccggatc gcatcgctgc ccgcggcgtc gagcttgcgc gatctcggca ttccggacga
     2581 ggaattgata ctctggcgcg gc
//
LOCUS       DATANS010000718         1696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_288735, whole genome shotgun sequence.
ACCESSION   DATANS010000718 DATANS010000000
VERSION     DATANS010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288735"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1391
                     /locus_tag="VHT22_10725"
     CDS             <1..1391
                     /locus_tag="VHT22_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX3138075.1"
                     /translation="TVEGSRVYDPEKDGDPVINNLERRTFRFFWDTTNKQNGLAPDRY
                     PSPSFASTAGIGFALTAYVIGSERGFVSRSQARLRTLRTLRFLANAPQGPDETGMTGY
                     RGFYYHFLHMPTGQRFGTSELSTVDTALLLGGVLAAQMYFDRDTRAEKEIRDLAELIY
                     TRVEWTWAQNRPPILSHGWRPETGFLRFDWRGYNEAMLVYILALGSPTHPIEPEAWKE
                     WLATYKNSWGTFSGQEFLSFGPMFGHQYSHVWIDFRGIQDEFMREKGIDYFENSRRAA
                     YAHREYSVQNPMRWRGYDSEIWGLTASDGPADTVQVFNNEERGFFTYAARGTAATHVL
                     DDGTIAPTAAIGSIAFAPEIVIPTIRAFRVRFGESLYQKYGFLDAVNPSFTFADVPLR
                     HGQLVDNVGWVDSDYLSIDQGPIVAMIENYRSGLIWKLMQRNPHIRRGLERAGFTGGW
                     LAQPPPPQQPSS"
     gene            1396..>1696
                     /locus_tag="VHT22_10730"
     CDS             1396..>1696
                     /locus_tag="VHT22_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3138076.1"
                     /translation="MVNRRQTMLALAAFAAAPLSCSRRSHGANTVRFWAVGREGEYAV
                     ELLQDFLRERPDIEVDVQKLPWTAAHEKLLTAYAGDTLPDLCSLGNTWIPEFHALN"
BASE COUNT          289 a          565 c          547 g          295 t
ORIGIN      
        1 tcacggttga gggttctcgc gtgtacgacc ctgagaagga tggcgatccc gtcatcaaca
       61 atctcgaacg gcgcaccttc cgtttcttct gggacacgac gaacaagcag aacggcctcg
      121 cgccggaccg ctatccctcg ccatcgttcg ccagcaccgc gggtatcggc tttgcgctca
      181 ctgcgtatgt gatcggctcg gagcgcggct tcgtgtcgcg cagccaggcg cggttgcgca
      241 cgttgcgcac gctgcggttc ctcgcgaacg cgccgcaggg ccccgacgaa accggcatga
      301 ccggctatcg tggcttctac tatcacttcc tgcacatgcc gacaggccag cgcttcggca
      361 cttcggagct gtcgaccgtc gacacggcgc tactgctcgg cggcgtgttg gcagcgcaga
      421 tgtacttcga tcgcgacacg cgcgccgaaa aggagatccg cgacctcgcg gagctgatct
      481 acacgcgcgt cgagtggacc tgggcgcaga accgcccgcc gatcctgtcg cacggctggc
      541 ggccggagac cggtttcctg cgcttcgact ggcgtggcta caacgaggcg atgctcgtct
      601 acatcctcgc gctcggctcg ccgacgcatc cgatcgagcc ggaggcgtgg aaggaatggc
      661 tcgccaccta caagaactcg tggggcacgt tcagcgggca ggaattcctg tcgttcggac
      721 cgatgttcgg ccaccagtac tcgcacgtct ggatcgactt ccgcggcatc caggacgagt
      781 tcatgcgcga gaagggcatc gactacttcg agaacagccg gcgcgcggcg tacgcgcatc
      841 gcgagtactc ggtccagaac ccgatgcgct ggcgcggcta cgacagcgag atctggggcc
      901 tgaccgcttc cgacggcccc gcggacacgg tgcaggtctt caacaacgaa gagcgcggct
      961 ttttcacgta tgcggcccgc ggcactgcgg cgacacatgt tctcgacgac ggcacgattg
     1021 cgccgactgc ggcaatcggc tcgatcgcgt tcgcgccgga gatcgtgatc ccgacgatcc
     1081 gcgcattccg cgtgcgcttc ggcgagagcc tgtatcagaa gtacggcttc ctcgatgcgg
     1141 tgaacccgag cttcaccttc gccgacgtgc cgctgcgtca cggccagctc gtcgacaacg
     1201 tcggctgggt cgacagcgac tacctcagca tcgaccaggg accgatcgtg gcgatgatcg
     1261 agaactaccg cagcggcctc atctggaagc tgatgcagcg caatccgcac atccgtcgcg
     1321 gcctggagcg tgccggcttc accggcggtt ggctcgcgca gccgccgccg ccgcaacagc
     1381 cgagctcgtg attccatggt gaatcggcgg cagacgatgc tcgcgctggc cgcgtttgca
     1441 gcggcgccgc tcagttgctc gcgtcgctcg cacggagcga acaccgtgcg cttctgggcg
     1501 gtcgggcgcg aaggcgagta tgcggtcgag ctgctccagg acttcctgcg tgagcgaccc
     1561 gacatcgaag tcgacgtgca gaagctgccc tggactgcgg cgcacgagaa gctgctgacc
     1621 gcttacgccg gcgacacgtt gccggacctg tgctcgctcg ggaacacgtg gatcccggaa
     1681 tttcacgcgt tgaatg
//
LOCUS       DATANS010000719         1742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_372477, whole genome shotgun sequence.
ACCESSION   DATANS010000719 DATANS010000000
VERSION     DATANS010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372477"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1742)
                     /locus_tag="VHT22_10735"
     CDS             complement(<1..>1742)
                     /locus_tag="VHT22_10735"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3138077.1"
                     /translation="LLKSYCRIEPRDDARTIREKLTGKLLTLDRNMESDLPAFLALLD
                     VPVGDGAWQEFDPPKRRKQTIDALKRLLLRESIEQPLLLVFEDLHWIDSETQALLDAL
                     IEGLPTARILLLVNYRPEYRHGWGGKTCYAQIRVDPFGVEGAEALLAALLGAATDVGK
                     PAAAGSAGRAHGGVALNDLKRLLIQRTEGNPFFLEESVRSLVETGTLTGERGSYRLKR
                     KFADIRVPATVQAILAARIDRLPAQEKRLLQTMAVVGKDVPYLLLRAIAAAEGARDAL
                     TEDALRIGLGHLQAAEFIYEVNLFPTLEYTFKHALTYEVAYESLLQERRKSLHARIVR
                     AFETLYPDRLAEHVERAVHHAVRGEMWEHALRYARQAGLKGVARSANREAVTFFEQAL
                     SALKLLPESRSTLEQAIDLRLDMRQALVPLGEFDQILSHLREAETIAKTLDDKRRLAR
                     ILSWMAYSYFFTLGDHDRAIETGRRALELSRELADTPLQVIATFYLAYPHQQRGDYRQ
                     AVEGLKQVVATLQGELISQRFGMAGYPAVLSRGLLAWSLGDLGEFAEGRIYADEAIAL
                     AEARNQPWSQGVAQ"
BASE COUNT          302 a          577 c          551 g          312 t
ORIGIN      
        1 ctgcgcaacg ccctggctcc agggctggtt gcgtgcttcg gccagcgcaa tcgcctcgtc
       61 ggcgtagatc ctcccttcgg cgaattcgcc cagatcgccg agggaccatg cgagcagccc
      121 gcgcgagagc accgccgggt atccggccat cccgaaacgc tgcgagatca gctcgccttg
      181 caaggtcgca acgacctgct tcaggccttc gaccgcctgc cggtagtcgc cgcgctgctg
      241 gtgcggatag gcgaggtaaa acgtcgcgat gacctgcaag ggcgtatcgg ccagctcgcg
      301 gctgagctcc agcgcgcggc gtccggtttc gatggcgcga tcatgatcgc cgagcgtgaa
      361 aaaatacgag tacgccatcc aggaaaggat tcgtgcgagc ctgcgcttgt cgtcgagggt
      421 cttggcgatc gtctcggcct cgcgcagatg gctcagaatt tgatcgaact caccgagggg
      481 aaccagcgct tgccgcatgt ccaggcgcag atcgattgcc tgctccagcg tgctccggct
      541 ttcgggaaga agcttcaacg cagacagcgc ctgctcgaaa aatgtcaccg cctcgcggtt
      601 agccgaacgg gcgacgccct tgagaccggc ctggcgcgca taacggagcg cgtgctccca
      661 catttctccg cgaaccgcat gatggaccgc gcgctcgacg tgctcggcca gccgatccgg
      721 atagagcgtc tcgaacgcac gcacgatgcg ggcgtgcagc gatttcctcc gctcctgcag
      781 caggctctcg tatgcgacct cgtacgtcag cgcatgcttg aacgtgtatt ccagcgtcgg
      841 gaaaaggttt acctcgtaga tgaactcggc ggcctgcaga tgcccgaggc cgatcctgag
      901 cgcatcttcg gtcagcgcat ccctggcgcc ttccgctgcg gcgatcgcgc gcaacagcag
      961 gtacggcaca tccttgccta cgactgccat cgtttgcagc agccgtttct cctgcgctgg
     1021 caggcgatcg atccgggccg cgagaatcgc ctgcaccgtc gccggcacgc gaatgtcggc
     1081 aaacttccgc ttcaaccggt aggagccccg ctcgccggtc agcgtgccgg tctcgacgag
     1141 tgagcgaacg ctttcctcga ggaaaaacgg gttgccttcg gtccgctgga taagcagccg
     1201 cttcaagtcg ttcaacgcga cgccgccgtg cgcgcgtccg gctgacccgg cggctgccgg
     1261 cttgccgacg tcggtcgccg cgcccagcag ggcggcgagc agcgcctcgg caccttccac
     1321 gccgaacggg tcaacgcgaa tctgcgcata gcaggtcttg cctccccagc cgtgccggta
     1381 ctcggggcgg taattgacca gcagaaggat gcgcgccgtg ggcaggcctt cgatcagcgc
     1441 gtcgagcagc gcctgcgtct ccgagtcgat ccagtgcagg tcctcgaaga ccagaagcaa
     1501 cggctgctcg atgctttcgc gaagcagcag gcgtttgagt gcatcgatcg tctgcttgcg
     1561 gcgcttcggc ggatcgaatt cctgccaggc gccgtcgccg accggcacgt cgagcaacgc
     1621 caggaacgcc ggaagatcgg actccatgtt gcgatcgagc gtcagtagct tgccggtcag
     1681 cttttctcgt atcgtgcgcg catcatcgcg aggctcgatg cggcaatagc tcttcaggag
     1741 at
//
LOCUS       DATANS010000720         3326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_244211, whole genome shotgun sequence.
ACCESSION   DATANS010000720 DATANS010000000
VERSION     DATANS010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244211"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /locus_tag="VHT22_10740"
     CDS             complement(<1..1587)
                     /locus_tag="VHT22_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3138078.1"
                     /translation="MADKESDALPGQVNLSAPFIARPVATTLLTIGLALAGIAAFRLL
                     PVSPLPQVDFPTISVSASLPGASPETMAATVATPLERSLGRIAGVTEMTSSSSLGSSR
                     VTLQFELSRDIDGAARDVQAALNAARSLLPTGLPGNPTYRKVNPADAPIMILALTSDT
                     MTQGQMYDAGSTIIAQKLSQLSGVGDVQVGGSSLPAVRVELDPPKLNKYGIGFEDVRT
                     VLAATNANRPKGSLEDGDRHWQIQANDQAKKAVDYLPLIVAYRNGAAVRLTDLGQVVD
                     SVQDLRNAGSSNGKPSVLIIISRQPNANIIETVDSVTEMLPQIRASIPSAIKLDVVME
                     RTTTIRASLRDVERTLIVAVCLVVIVVFVFLRHWRATLIPSVAVPVSLVATFGAMYLF
                     GFSLNNLSLMALTIATGFVVDDAIVVLENTSRHIEQGMDRFEAALVGAREVGFTVLSI
                     SLSLVAVFIPLLFMGGQAGRLFREFAVTLSVAVLISLVVSLTTTPMMCAWLLDRDRVT
                     GATRPRGRFARVSETAYQRVL"
     gene            complement(1589..>3326)
                     /locus_tag="VHT22_10745"
     CDS             complement(1589..>3326)
                     /locus_tag="VHT22_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3138079.1"
                     /translation="TEKEKQGKLYRRSGEIFDRLIERYGEMLNWVLDRQVATLVVAAI
                     TLVLTVVLYIFIPKGFFPIQDTGAIQGITEAPQTVSFAAMAERQQALAAVILQDPAVE
                     SLSSFIGVDGTNVTLNSGRMLINLKPLSQRHGRATDVIRDLQARVASVEGIALYMQPV
                     QDLTIEDRVSRTQYQFTIEDTNPDELSVWVPRIVERLRHIPQLADVASDLQDGGLQAY
                     VDIDRATASRYGITPAAIDNALYNAFGQRLVSTIFTQTNQYRVVLEVKPEFQLGPEAL
                     SQIYVPVGNVSSAPASVGANASAALAAPGAGASPAGASQQSPQQVPLTSIARISEHAT
                     ALVVSHNGQFPAATISFNLAPGASLGNAVEEIQKAQQELALPASAQLRFQGAAAAFRG
                     SLANELWLILAAIVTMYIVLGVLYESYIHPITILSTLPSAGVGALLGLMIFGKDLDVI
                     AIIGIVLLIGIVKKNAIMMIDFALDAERKEGKPPREAIFQACLLRFRPIMMTTMAALL
                     AALPLMLGWGVGSELRHPLGITMVGGLIVSQMLTLFTTPVIYLAFDRLARRVEGRRGR
                     KAGSEPVPRAGD"
BASE COUNT          575 a         1086 c         1104 g          561 t
ORIGIN      
        1 cagcacgcgc tggtaggccg tttcgctcac gcgcgcaaat cgcccgcgcg gtcttgtcgc
       61 ccctgtgacg cggtcgcgat cgagcagcca cgcgcacatc atcggcgtcg tcgtcagcga
      121 gaccacgagc gagatcagca ccgcgaccga cagcgtcacc gcgaactcgc ggaacagccg
      181 gccggcctgg ccccccatga acagcagcgg gatgaacacc gcgaccagcg acaggctgat
      241 cgacagcacc gtgaagccga cctcgcgcgc gccgaccagc gcggcctcga agcggtccat
      301 gccctgctcg atgtggcggc tggtgttctc gagcacgacg atcgcatcgt cgacgacgaa
      361 gccggtggcg atcgtaagcg ccatcagcga gaggttgttc aggctgaacc cgaagaggta
      421 catcgccccg aaagtcgcga ccagcgacac cggcacggcg acgctcggga tcagcgtcgc
      481 ccgccaatgg cgcagaaaaa cgaacacgac aatcactacc aggcacaccg cgacgatcag
      541 tgtgcgctcg acgtcgcgca gcgacgctcg tatcgtcgtc gtacgctcca tcacaacgtc
      601 gagcttgatc gcgctcggga tcgaggcgcg gatctgcggc agcatttcgg tcacgctgtc
      661 gactgtctcg atgatgttcg cgttcggctg gcggctgatg atgatcagca ccgaaggctt
      721 gccgttcgac gatccggcgt tacgcaggtc ctgcaccgag tcgacgacct ggccgagatc
      781 ggtgagccgg accgcggcgc cgttgcgata ggcgacgatc agcggcagat agtcgacggc
      841 ttttttcgcc tggtcgttcg cctggatctg ccaatggcga tcgccgtcct cgagcgagcc
      901 cttcggccgg ttggcattgg tcgcggcgag caccgtgcgc acgtcctcga agccgatgcc
      961 gtacttgttg agcttcggcg gatcgagctc gacccgcacc gccggcagcg agctgccgcc
     1021 gacctgcacg tcgccgacgc cggacaattg cgagagcttc tgcgcaatga tcgtcgagcc
     1081 ggcgtcgtac atttgtccct gcgtcatcgt gtcggacgtc agcgccagga tcatgatcgg
     1141 tgcgtcggcc ggattgacct tgcgataggt cggattgccc gggagcccgg tcggcaagag
     1201 gcttctcgcc gcgttgagtg cggcctggac gtcgcgcgct gcgccatcga tgtcgcgtga
     1261 aagctcgaat tgcagggtga cacgcgacga acccagcgag ctgctcgacg tcatctcggt
     1321 cacgccggcg atacggccga gcgagcgctc gagcggtgtg gcgacggtcg cggccatcgt
     1381 ttcagggctc gcgccgggca gcgacgccga caccgaaatc gtaggaaagt cgacttgcgg
     1441 aagcggcgaa accggcagca ggcggaacgc cgcgatgccc gcgagcgcca ggccgatcgt
     1501 cagcagcgtc gtcgcgaccg gccgcgcgat gaatggcgcc gacaggttga cctgtccggg
     1561 aagcgcgtcg gattctttgt cggccatgtc aatcgccggc ccgaggcacg ggctcggatc
     1621 cggccttccg gccgcggcgc ccttcgaccc ggcgagcgag tcggtcgaac gcgaggtaga
     1681 tgaccggcgt ggtgaacaag gtcagcatct ggctgacgat caggccgccg accatcgtga
     1741 tacccagcgg atggcgcaat tcggagccca caccccagcc cagcatcagc ggcagcgcgg
     1801 cgagcagcgc tgccatcgtg gtcatcatga tcgggcgaaa gcgcagcagg cacgcctgga
     1861 agatcgcctc gcgcggaggc ttgccttcct tgcgctcggc gtcgagcgcg aagtcgatca
     1921 tcatgatcgc gtttttcttg acgatgccga tcaggagcac gatgccgatg atggcgatga
     1981 cgtcgaggtc cttgccgaag atcatcaggc cgagcagcgc accgacgccc gcggaaggca
     2041 gcgtcgacag gatcgtgatt ggatggatgt agctttcgta cagcacgccc agcacgatgt
     2101 acatcgtgac tatcgcggcg aggatcagcc agagctcgtt ggccagcgag ccgcgaaacg
     2161 ccgcggcggc gccctgaaaa cgcaattgcg cgctggccgg cagcgcgagc tcctgctgcg
     2221 ctttctggat ttcctcgacc gcattgccga gcgacgctcc gggcgccagg ttgaacgaga
     2281 ttgtcgccgc cggaaactgg ccgttgtgac tgacgaccag cgccgtcgcg tgctcgctga
     2341 tccgcgcgat cgacgtaagt ggcacctgtt gcggggattg ctggctcgcg cccgccggac
     2401 tggcgcccgc gcccggcgcg gccagcgccg cggaggcgtt ggccccgacc gacgccggcg
     2461 ccgacgagac gttgccgacc ggcacataga tctgcgacag cgcttccggc ccgagctgga
     2521 actcgggctt tacttccagc acgacgcggt actggttcgt ctgcgtgaat atcgtcgaca
     2581 ccagtcgctg gccgaacgcg ttgtacaagg cgttgtcgat cgcggccgga gtgatcccgt
     2641 agcggctcgc cgtcgcgcgg tcgatgtcga cgtaagcctg caggccgccg tcctgcaggt
     2701 cgctggcaac gtcggccagc tgcggaatat gacgcaaacg ctcgacgatg cgcggaaccc
     2761 agacgctcaa ttcgtccggg ttcgtgtcct cgatcgtgaa ttggtattgc gtccggctga
     2821 cccggtcctc gatcgtcaga tcctgcaccg gctgcatgta cagcgcgatg ccttcgacac
     2881 tggcgacacg cgcttgcaga tcgcggatga cgtcggtggc gcgcccgtgc cgctgcgaga
     2941 gcggcttcag gttgatcagc atccggccgc tgttcagggt cacgttggtt ccgtcgacgc
     3001 cgatgaacga cgagaggctc tcgaccgcgg ggtcctgcag gatgacggcg gcgagtgcct
     3061 gctgccgctc cgccatcgcg gcgaacgaga ccgtttgcgg cgcctcggtg atgccctgga
     3121 tcgccccggt gtcctggatc ggaaagaagc ctttcggaat gaatatgtac aacacgaccg
     3181 tcagtaccag cgtgatcgcg gcgacgacca gggttgcgac ctgccggtcg agcacccagt
     3241 tcagcatctc gccgtagcgc tcgatcagtc gatcaaagat ctcgccgctt cgccggtaga
     3301 gcttgccctg cttttccttt tcggtg
//
LOCUS       DATANS010000721         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_77315, whole genome shotgun sequence.
ACCESSION   DATANS010000721 DATANS010000000
VERSION     DATANS010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77315"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..1282
                     /locus_tag="VHT22_10750"
     CDS             8..1282
                     /locus_tag="VHT22_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138080.1"
                     /translation="MSQDLQSLPPAEQRNLANGFVEILRGRKIFRFDTFGDQAFWGDT
                     LGLHRAIAGQANGGVGAGLSPKAALELGLKVDVNALPHSLRNELRHGRVDLNDPATTL
                     ALLKLDAVVGVRGFFDERNRRLESVGITCALCHSTVDNSFAPGIGNRLDGWAARDLNV
                     GAIIAFSPSVQPFVDLLRLAQPGIDAAAVRSVLRSWGPGRFDAELLLDGKAFRPDGKS
                     ASTLIPPAFGLAGVNMHTWTGWGSVTHWNAFVANLEMQGSGTFYDPRLNDATKFPIAA
                     AAGFADVRKDPDLITGKLAALHVFQLSLPAPRAPSSSFNAAAAQRGKELFAGKADCAR
                     CHVPPLYTEPGWNMHTPEEIGIDAFQAQRAPDERYRTAPLKGLWTHAKGGFYHDGRFA
                     SLRDVIDHYDRQFALGLSAAEKTDLAEYLKSL"
     gene            complement(1286..>2099)
                     /locus_tag="VHT22_10755"
     CDS             complement(1286..>2099)
                     /locus_tag="VHT22_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138081.1"
                     /translation="AAAPAMIAQLVGNPSTAHMQLAREIRESLPEPVVAAGRHPQSAR
                     ALLLALALDSNPEIRARQKQVIGVRLSAQVAAMTAALEPQVDALEPEQRIPALLRGFP
                     TLRQLTHEERVQLIACLNGMLPRDAEVSLHSYVLRKLAQMHLRDDLNPRARPSRLTLQ
                     AVQQDAQVLFSVLAQHGHDDETSARRAYEAGMHHLFPRARPGYGLSGHWPPALDVALS
                     RLDQLAPIGKEQLVEAMVATVTHDQQLTMGEAELLRAVCASLHCPLPPLVAS"
BASE COUNT          353 a          695 c          691 g          360 t
ORIGIN      
        1 cgcgcggatg tcgcaggacc tgcaatccct gcctccggcc gagcagcgca acctcgcgaa
       61 cggcttcgtg gaaatcctgc gcggtcgcaa gatcttccgt ttcgacacgt tcggcgacca
      121 ggctttctgg ggcgacacgc tcggcctgca tcgtgcgatc gccggccagg cgaacggcgg
      181 tgtcggtgcg gggctgagcc cgaaggcggc gctcgagctc ggcctcaagg tcgacgtcaa
      241 tgcgctgccc cattcgctgc gcaacgagct gcgccacgga cgcgtcgatc tgaacgatcc
      301 cgccacgact ctcgccttgc tgaagctcga tgcggtcgtc ggcgtgcgcg gcttcttcga
      361 cgagcgcaat cgacggctcg aatccgtcgg cataacctgc gcgctgtgcc actcgacagt
      421 cgacaactcg ttcgcgcccg gcatcggcaa ccggctcgac ggttgggctg cgcgcgatct
      481 gaacgtcggc gcaatcatcg cgttctcgcc gagcgtgcaa ccgttcgtgg atctgctgcg
      541 actggcgcag ccgggcatcg acgcagcagc cgtacgctca gtgctgcgca gctggggtcc
      601 gggtcggttc gacgcagagc tgttgctcga cggcaaggcg ttccgccctg atggcaagtc
      661 ggcctcgacg ctgattcctc cggcgttcgg cctagccggc gtcaatatgc acacctggac
      721 cggctggggc tcggtcacgc actggaacgc gttcgtggcg aacctggaaa tgcagggcag
      781 cggcacgttc tacgatccgc ggctcaacga tgcaaccaag tttccgatcg ccgctgctgc
      841 gggcttcgcc gatgtgcgca aggatcccga cctgatcact ggcaaactgg cggcactgca
      901 cgtctttcaa ttgagtttgc cggcgccgcg agcaccgtcg agcagcttca atgcagcggc
      961 tgcgcagcgt ggcaaagagc tgttcgcggg caaagctgat tgcgcccgtt gccacgtccc
     1021 gccgctctac acggagccgg gctggaacat gcacacgcct gaggagatcg gcatcgacgc
     1081 attccaggcg cagcgcgcac ccgacgagcg ctatcgcacg gcgccgctca aaggcttgtg
     1141 gacgcacgcg aagggcggct tctatcacga tggacgcttt gcgtcgctgc gcgatgtgat
     1201 cgatcactac gatcgtcagt tcgcgctcgg gctgagtgcg gcggagaaga cggatcttgc
     1261 cgagtatctg aagtcgctgt aagcgtcagc tcgcgaccag cggcggcaac gggcaatgca
     1321 ggctcgcgca caccgcgcgc agcagttccg cctcgcccat cgtcagctgc tggtcgtggg
     1381 tcaccgtcgc gaccatcgct tcgaccagct gctccttgcc gatcggcgcg agctgatcga
     1441 gccggctgag cgcaacatcg agcgccggcg gccagtgtcc ggagagaccg tagccaggcc
     1501 tggcgcgcgg aaacaagtga tgcatgccag cttcgtacgc gcggcgcgca ctcgtctcgt
     1561 cgtcgtgacc gtgttgcgcg agaacggaaa acagaacctg cgcgtcctgc tgcacggctt
     1621 gcaacgtcag gcgcgaggga cgcgcacgag gattcagatc gtcgcgcaag tgcatctgcg
     1681 caagcttgcg cagaacgtaa ctgtgcagcg agacctccgc gtcccgcggc aacatgccgt
     1741 tgaggcatgc aatcaactgc actcgctctt cgtgcgtgag ctgtcgcagc gtcgggaagc
     1801 cgcgcagcaa ggctggaatg cgctgctccg gctcgagcgc atcgacctgc ggttcgagtg
     1861 ccgcagtcat cgcagcgact tgcgccgaca gccgtacccc aatcacctgc ttctgccgtg
     1921 cacggatctc gggattcgaa tcgagcgcga gcgcgagcag cagcgcgcgt gccgactgcg
     1981 gatgtcgacc ggccgcgacg actggctcgg gcagcgattc gcgaatttct cgcgcgagct
     2041 gcatgtgcgc cgtgctcgga ttgcccacga gctgcgcgat catcgcggga gctgcggcc
//
LOCUS       DATANS010000722         1794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_831545, whole genome shotgun sequence.
ACCESSION   DATANS010000722 DATANS010000000
VERSION     DATANS010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831545"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VHT22_10760"
     CDS             <1..418
                     /locus_tag="VHT22_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX3138082.1"
                     /translation="WIDQVSFVAETGSGHAVVVDGSPDAGGRNIGPRPMELVLAGTAA
                     CTAFDVVWILKKARQPITGCIVDAEVERAPTEPKVFTRMHFVYRISGEGLNPAQVERA
                     VQLSKEKYCSATLMLAKTAAISFEIIINGEPLEPTP"
     gene            complement(415..1287)
                     /locus_tag="VHT22_10765"
     CDS             complement(415..1287)
                     /locus_tag="VHT22_10765"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3138083.1"
                     /translation="MESGKLAFLGLGVMGFPMAGHLARAGHSVVVYNRTPAKSAQWVE
                     KYGGSAAPTPAAAAADAALVMMCVGNDDDVRAVALGPKGAVAAMRAGSVLVDHTTASA
                     TVAREVHTAAKARGVGFLDAPVSGGQAGAENGKLTIMVGGEADTFARAEGVLAHYARA
                     VTLMGGPGSGQLTKMVNQICIAGLVQALSEGINFAQKAGLDAERVLDVIGKGAAQSWQ
                     MDNRGKTMVADKFDFGFAVDWMRKDLAICAAEARSNGAALPVTALVDQFYARIQAQGG
                     ARWDTSSLIRLLRG"
     gene            complement(1339..>1794)
                     /locus_tag="VHT22_10770"
     CDS             complement(1339..>1794)
                     /locus_tag="VHT22_10770"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112392.1"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX3138084.1"
                     /translation="QPLAYNKDNQEDKAPVFDVVDTLLPTLGVLAALVSDGIEVDAER
                     MRAAASEGYATATDLADYLVKKGLPFRDAHEAVAQAVRHAESEQCDLATLSLASLQRY
                     SPLIENDVYAVLTLEGSIASRDHPGGTAPAQVRAAIRAARSSLEPGKAR"
BASE COUNT          293 a          624 c          577 g          300 t
ORIGIN      
        1 atggatcgac caggtcagct tcgttgccga aaccggcagc ggccacgccg ttgtcgtgga
       61 cggttcgccc gacgccggcg ggcggaacat cgggccgcgg ccgatggaac tcgtactggc
      121 gggcaccgca gcatgcacgg cattcgatgt cgtctggatc ctgaaaaagg cgcgccagcc
      181 gatcaccggc tgcatcgtcg atgccgaggt cgagcgcgcg ccgaccgagc ccaaggtgtt
      241 cacccgcatg cacttcgtct atcggattag cggcgaaggc ttgaacccgg cgcaggtcga
      301 gcgcgcggtg cagctgtcga aggaaaaata ttgctcggcg acgctgatgc tggccaagac
      361 tgccgcgatc agcttcgaga tcatcatcaa cggcgagccg ctcgagccga ccccctagcc
      421 ccgcaacaga cgaatcaggc tcgacgtgtc ccagcgtgcg ccgccctgcg cctgaattcg
      481 cgcgtagaac tggtcgacca gcgcagtcac cggcagcgcc gcgccgttgc tccgcgcttc
      541 ggccgcgcat atcgcgagat ccttgcgcat ccagtcgacg gcaaagccga agtcgaactt
      601 gtcggcaacc atcgtcttgc cgcgattgtc catctgccag gattgcgccg cgcctttgcc
      661 gataacgtcg agcacgcgct cggcgtcgag cccggccttc tgggcgaagt tgatcccttc
      721 cgacaacgcc tgcaccaatc cggcgatgca gatctggttg accatcttgg tcagctgacc
      781 tgagcccggg ccgcccatca aggtcactgc gcgcgcgtaa tgcgccagca cgccttcagc
      841 acgggcaaag gtgtcggctt cgccgccaac catgatcgtc agcttgccgt tttccgcacc
      901 ggcctggccg ccggataccg gcgcgtcgag gaagccgacg ccgcgagcct tcgccgccgt
      961 gtgcacctcg cgcgcaacgg tcgccgacgc ggtcgtgtga tcgaccagga cgctgccggc
     1021 gcgcatcgct gcaaccgcgc ctttggggcc cagcgcgacg gcgcgaacgt cgtcgtcgtt
     1081 gccgacgcac atcatcacca gcgccgcatc ggctgcggca gcggcggggg tcggcgctgc
     1141 gctgccaccg tacttttcga cccactgcgc ggacttggcc ggcgtgcggt tgtaaacaac
     1201 caccgaatgt ccggcgcgcg ccagatgtcc ggccatcgga aagcccatta cacccaggcc
     1261 cagaaacgcc agtttgccgc tttccatcgc tgctccttgc tggttgagac ggggccggtg
     1321 cggcccggtg gctggccgct agcgcgcctt gccgggttca agcgaggacc gcgcggctcg
     1381 aatcgccgcg cgcacctgcg ccggggccgt cccgccggga tgatcgcggc tcgcgatcga
     1441 gccttcgagc gttagcaccg catagacatc gttttcgatc agcggcgaat agcgctgcag
     1501 cgaggcgagc gacagcgtgg ccagatcaca ctgctcggat tcggcatgcc gcactgcttg
     1561 cgcgaccgcc tcgtgcgcgt cgcggaacgg cagccccttc ttgaccaggt aatcggcaag
     1621 atcggtcgcg gtcgcgtagc cctcgctggc cgccgcgcgc atgcgctcgg catcgacttc
     1681 gatgccgtcg gataccagcg cggcaaggac gcccagcgtc ggcagcagcg tgtcgacgac
     1741 gtcgaatacc ggcgccttgt cctcctggtt gtccttgtta taagcgagcg gttg
//
LOCUS       DATANS010000723         1540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_289445, whole genome shotgun sequence.
ACCESSION   DATANS010000723 DATANS010000000
VERSION     DATANS010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289445"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..956
                     /locus_tag="VHT22_10775"
     CDS             48..956
                     /locus_tag="VHT22_10775"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3138085.1"
                     /translation="MADSSSAGLATGYPSQQHRARLALLLLIAPALVWLVGLIVLPHL
                     ELAVLSLRARVAPRVYEFSFNQYRTFFEEPLYWHTFVRTAGMSILATLCTLLISFPVA
                     WYIAKIARGRSKSVLFILCLIPFWVSETVRTLGWMILLRESGVLPRLLVDVGITTMPV
                     EMLYHDATILVGLVYTSMLFMVVPLVSALESLDDSLVEAAYDLGGDGWSILRQIVVPH
                     AAPGIAAGSIVVFMLTLGNYLTPTLLGGKNSLWFTEQIYTQFITRFNWEQGAAFGFLL
                     LVLSTAIVWLGLKLTRQRFGDVMRRE"
     gene            953..>1540
                     /locus_tag="VHT22_10780"
     CDS             953..>1540
                     /locus_tag="VHT22_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3138086.1"
                     /translation="MIPSLPRSALSRYSIGGYVVAFFVFLFVPLVVVAVFAFNDANYP
                     APPWRGFTFDWFLGGSGGRTGVFADGPLLKSVGTSVIVAGWVTLLAVAVGTANAFLLE
                     RARFRGKSALSLLMLAPLVIPGVILGISILAFASRVAQFADDVWGWDLDALRPGLPLV
                     VAGQFSYIVSIATLTIAARLKRFDATLEEAAFNLGA"
BASE COUNT          208 a          484 c          503 g          345 t
ORIGIN      
        1 gttcggcgtc gacccgcagc gcgcggtctg cttccgcgca gggaatcatg gcggatagtt
       61 cgtccgccgg cctggcgacc ggatacccgt cgcaacagca tcgtgcgcgc ctcgcgctgt
      121 tgctgcttat cgcgccggcg ctggtctggc tcgtgggcct gatcgtgctg ccgcatctcg
      181 agcttgcggt gctgtcgctc agggcacgcg tggcgccgcg cgtatacgag ttcagtttca
      241 accagtatcg gaccttcttc gaagagccat tgtattggca caccttcgtg cgcacggccg
      301 ggatgtcgat cctggccacg ctgtgcacct tgctgatctc gtttcctgtt gcctggtaca
      361 tcgcgaagat cgcgcgcggc cggtctaagt cggtgctttt catcctgtgc ctgatcccgt
      421 tctgggtaag cgaaaccgta cgcacgctag gctggatgat cctgctgcgc gaatcgggcg
      481 tgctgccgcg cctcttggtc gacgtcggga tcaccacgat gcccgtggaa atgctctatc
      541 acgatgcgac catcctcgtc ggcctcgtct acacctcgat gctgttcatg gtcgtgccgc
      601 tggtcagcgc acttgaaagc ctggacgatt cgctggtcga ggccgcttac gacctcggcg
      661 gtgacggctg gtcgatcctg cgacagatcg tcgtgccgca cgcagcaccc gggatcgccg
      721 ccggatcgat cgtcgttttc atgctgacgc ttggcaacta tctgacgccg acgctgctcg
      781 gcggcaagaa ctcgctctgg ttcaccgagc agatctatac gcaattcatc acgcggttca
      841 actgggagca aggcgcggct ttcggtttcc tgctgctcgt gctgtcgacg gcgatagttt
      901 ggctcggctt gaagctgacc cggcagcgct tcggcgacgt gatgaggcgc gaatgatccc
      961 atcgcttccg cgctcagcgc tatcccggta ctcgataggc gggtatgtcg ttgcattctt
     1021 cgtgttcctg ttcgtgccgc tggtcgttgt cgcggtgttc gcgttcaatg acgcgaatta
     1081 tccggcgccg ccgtggcgtg gtttcacatt cgactggttt ctgggtggca gtggcggacg
     1141 tactggcgtc ttcgccgacg gcccgctgct caagagtgtg ggcaccagcg tcatcgttgc
     1201 cgggtgggtg acactgctgg cggtagcggt cggcacggcg aacgcgttcc tgctcgagcg
     1261 cgcacggttc cgaggcaagt cggcgctgtc gctgctgatg ctggcgccgc tggtgattcc
     1321 cggcgtgatc ctcggcatct cgatcctggc gttcgcgagc cgcgtcgcgc aattcgccga
     1381 cgacgtctgg ggatgggacc tcgacgcgct gcggcccggg ctccccctgg tggtggccgg
     1441 ccagttctcg tacatcgtgt cgatcgcgac gctcacgatc gccgcgcggc tgaagcgctt
     1501 cgatgcgacg ctcgaggagg cggcgttcaa cctgggcgca
//
LOCUS       DATANS010000724         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_498847, whole genome shotgun sequence.
ACCESSION   DATANS010000724 DATANS010000000
VERSION     DATANS010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498847"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2023
                     /gene="clpA"
                     /locus_tag="VHT22_10785"
     CDS             <1..2023
                     /gene="clpA"
                     /locus_tag="VHT22_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEX3138087.1"
                     /translation="FQRVIQRAILHVQSSGKKEVTGANVLVAVFGEKESHAVYFLNQR
                     GVTRLDVVNYIAHGITKTPQKGQEKQEPAKEDAEGEQQPAGALDAYTVDLNHLAKTGK
                     IDPLIGREYELERVIQVLCRRRKNNPLLVGEAGVGKTAIAEGLARRINDGNIPEMLKD
                     FVVHSLDMGALLAGTKYRGDFEQRLKAVLKQLTENPRTILFIDEIHTIIGAGAASGGT
                     LDASNLLKPALSTGALKCIGATTYNEYRVIFEKDHALSRRFQKIDVPEPSIAETIEIL
                     KGLKSRFEQHHGVKYAPAALTTAAELAARFINDRHLPDKAIDVIDEAGAAQKILPKSK
                     QKKVINKGEIEEIVAKIARIPAKSVSSDDRNALKTLDRDLKNVVFGQNLAIDALVAAI
                     RMARSGLGNPRKPIGNFLFSGPTGVGKTEVARQLAFCLGVELLRFDMSEYMERHAVSR
                     LIGAPPGYIGFDQGGQLTEAITKHPYSVLLMDEIEKAHPDIFNILLQVMDHGTLTDNN
                     GRKADFRNVIIIMTTNAGADVLSKSSIGFTSNKQAGDEMESIKRMFTPEFRNRLDATI
                     SFASLDHEVILRVVDKFLLELENQLHEKKVEPSFTPRLREFLAKKGFDPQMGARPMAR
                     LIQDTIRKALADELLFGRLVSGGKVIIDVGDDDKITLAFDESKEAAPVA"
BASE COUNT          457 a          675 c          684 g          335 t
ORIGIN      
        1 cttccagcgc gtcatccagc gcgcgatcct gcacgtccag tcgtccggca agaaggaagt
       61 caccggcgcg aacgtcctgg tcgccgtgtt cggcgaaaag gaatcgcacg ccgtctactt
      121 cctcaaccag cgcggcgtca ctcggctcga cgtcgtcaac tacatcgcgc acggcattac
      181 caagacgccg cagaagggcc aggagaagca ggagccggca aaggaagacg ccgagggtga
      241 gcaacagccg gcaggcgcgc tcgacgcgta cacggtagac ctcaatcacc tggccaagac
      301 cggcaagatc gacccgctga tcggacgcga atacgagctc gagcgggtca ttcaggtgtt
      361 gtgccgccgg cgcaagaaca atccgcttct ggtcggcgaa gccggggtcg gcaagaccgc
      421 gatcgccgag ggtctcgccc ggcgcatcaa cgacggcaac atcccggaga tgctgaagga
      481 cttcgtcgtc cattcgctcg acatgggtgc actgcttgcc ggcacgaaat accgcggcga
      541 tttcgagcag cgcctgaagg cggtgctgaa gcagctgacc gagaatccgc ggacgatcct
      601 gttcatcgac gagatccaca cgatcatcgg cgcgggcgcg gcttcgggcg ggacactcga
      661 cgcgtcgaac ctgctgaagc cggcgctgtc gaccggcgcg ctgaagtgca taggcgcgac
      721 cacgtacaac gaatatcgcg tgattttcga aaaggaccac gcgctgtcgc gtcgcttcca
      781 gaagatcgac gtgcccgagc catcgattgc cgagacgatc gagatcctca aggggctcaa
      841 gtcacgcttc gagcagcatc acggtgtgaa gtacgcgccc gcggcgctga ccaccgcggc
      901 cgagctcgcg gcgcgcttca tcaacgaccg gcatctgccc gacaaggcga tcgacgtgat
      961 cgacgaggcc ggcgccgcgc agaagatcct gccgaaatcg aaacagaaga aggtgatcaa
     1021 caagggcgag atcgaggaga tcgtcgccaa gatcgcgcgc attccggcca agagcgtgag
     1081 ctccgacgac cgcaacgcgc tgaagacgct cgaccgcgac ttgaagaacg tcgtcttcgg
     1141 ccagaacctg gcgatcgacg cgctggtcgc ggcgatccgc atggcgcgct cgggcctggg
     1201 caatccgcgc aagccgatcg gcaatttcct gttctcgggc ccgaccggcg tcggcaagac
     1261 cgaggtggcg cgccagctcg cgttctgtct cggtgtcgag ctgttgcgct tcgacatgtc
     1321 ggagtacatg gagcggcacg cggtttcgcg cctgatcggc gcgccgcccg ggtatatcgg
     1381 cttcgatcag ggcgggcagc tgaccgaggc gatcaccaag catccgtaca gcgtcctgtt
     1441 gatggatgaa atcgagaaag cgcacccgga catcttcaat atcctgctgc aggtgatgga
     1501 tcacggcacg ctgaccgaca acaacggccg caaggcggac ttccgcaacg tcatcatcat
     1561 catgaccacc aacgccggcg ccgacgtgct gtcgaagagc tcgatcggct tcaccagcaa
     1621 caagcaggcc ggcgacgaga tggaatcgat caagcgcatg ttcacgccgg agttccgcaa
     1681 ccggctggac gcgacgattt cgttcgcttc gctggaccac gaggtgatcc tccgcgtcgt
     1741 cgacaagttc ctgctagagc tggagaatca gctgcacgaa aagaaggtcg agccctcgtt
     1801 cacgccgcga ctgcgcgagt tcctggcgaa aaaaggattc gacccgcaaa tgggcgcgag
     1861 accgatggct cgcctgatac aggacacgat ccgcaaggcg ctggccgacg agctgctgtt
     1921 cggccggctg gtcagcggcg gcaaggtgat aatcgacgtc ggcgacgacg acaagatcac
     1981 gctcgcgttc gacgaatcca aggaggctgc accggtcgct tgaaactaac cgcactggtg
     2041 cgaacagcgg gagcctgcgg gctcccgttt gttttgctgg tcgcaggcgc gattgaattc
     2101 gacaccgaaa tcggcgacga tgcagcgatg aggtccgcat gcctgccgcg c
//
LOCUS       DATANS010000725         2288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_120200, whole genome shotgun sequence.
ACCESSION   DATANS010000725 DATANS010000000
VERSION     DATANS010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120200"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2191
                     /gene="mutS"
                     /locus_tag="VHT22_10790"
     CDS             <1..2191
                     /gene="mutS"
                     /locus_tag="VHT22_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX3138088.1"
                     /translation="LDGKRDCLLVAVHATASRAGIAWLNLAGGRLHLRDVPLAEIAAL
                     LARIDPSEILHAEDSAAIAFNGAPAHALPPWRFDRATGVRRLCTQFGTRDLAAFGADD
                     APLAVAAAGALLDYAQATQQAALAHVRTLTIERATDHLALDLATRRNLEITETLRGEP
                     APTLYSLLDSCCTSAGSRLLRSWLTNPLRAQSAAALRHAAIAELAAAPSRGAWRKALK
                     TTVDIERIASRIALRSARPRDLAGLRDTLQRLPALATSVAHCQSPLLVAAAAALAIES
                     RWAELLARAIAAEPAAAVREGNVIAAGYDAELDELRAIQDNCGAFLIDLEARERQRTG
                     IASLKVEFNRVHGFYIEVSNANVQRIPDDYRRRQTMKNAERYITPELKTFEDKALSAQ
                     ERALARERRLYEEIVAALEPAIEALQQAALALASLDVFTAHAEQAAALRWTQPRFSAE
                     PMLAIRGGRHPVVEEQVEHFIPNDLSLDAARRMLIVTGPNMGGKSTYMRQTAVIALLA
                     YCGLFVPATDAEIGALDAIHTRIGAADDLAGGRSTFMVEMTEAAYILNRATPSSLVLI
                     DEIGRGTSTFDGLALAWAIARELALKNRCLTLFATHYFELTALPAELETCANVHFDAV
                     EHKDGIVFLHAVEEGPANQSYGLQVARLAGVTATVIRGARAHLARLDHFSARRDGDLF
                     AAVAPAADPAPADSSLRDRLKALDPDAMSPRDAHEILYELKRLADDA"
BASE COUNT          349 a          816 c          774 g          349 t
ORIGIN      
        1 gctcgacggc aagcgcgatt gcctgctcgt cgcggtgcac gcgacggcct cgcgggccgg
       61 catcgcatgg ctcaatctgg ccggtggcag gctgcacctg cgcgacgtgc cgctggccga
      121 gatcgcggcg ctgctcgcgc gcatcgaccc cagcgagatc ctgcacgccg aagacagcgc
      181 tgccatcgca ttcaacgggg cgccggcgca cgcactgccg ccctggcgct tcgatcgcgc
      241 caccggtgtg cggcgcctgt gcacccaatt cggcacgcgc gacctcgccg cattcggcgc
      301 cgatgacgcg ccgctcgccg tcgccgccgc cggcgctctg ctcgactacg cgcaggccac
      361 gcaacaggca gcgctcgcgc acgttcgcac cctcacgatc gagcgcgcaa ccgatcatct
      421 ggcgctcgac ctcgcgacgc ggcggaatct ggagatcacc gagacgctgc gcggcgagcc
      481 tgctccgacg ctttattcgc ttctcgacag ctgctgcacc agtgccggca gccgcctgct
      541 gcggagctgg ctcaccaatc cgctgcgtgc ccagtccgcc gccgctttgc gccacgcggc
      601 gatcgccgag ctcgcggcgg caccatcgcg cggcgcgtgg cgcaaggcgc tgaaaacgac
      661 ggtcgatatc gagcgcatcg catcgcgcat cgcgctgcgt tcggcccggc cgcgcgacct
      721 cgccgggctg cgcgacacgc tgcagcgcct gccggcgctg gcgacgtccg tcgcccactg
      781 ccagtcgccg ctgcttgtcg cagccgcagc cgcgctcgcg atcgaatcgc gatgggcgga
      841 gctcctggcc cgagcgatcg ccgccgagcc cgctgccgcg gtgcgcgaag gcaacgtgat
      901 cgcggccgga tacgacgccg aactggacga gttgcgcgcg atccaggaca actgcggcgc
      961 gttcctgatc gacctcgaag cgcgcgagcg gcaacgcacg gggatcgcca gcctcaaggt
     1021 cgagttcaac cgcgtgcacg gcttctatat cgaggtatcg aacgccaacg tgcagcgcat
     1081 tcccgacgac taccggcggc ggcagacgat gaagaacgcc gagcgctaca tcacgccgga
     1141 gctcaagacg ttcgaggaca aggcgctgtc ggcgcaggag cgcgcgctcg cccgagagcg
     1201 caggctgtac gaggagatcg tcgctgcgct cgagccggcg atcgaggcgc tgcagcaggc
     1261 ggctctcgcg ctggcatcgc tcgacgtttt caccgcgcac gccgagcagg cggccgcgct
     1321 gcgctggacg caacctcgct tcagcgccga gcccatgctc gcgatccgcg gcgggcgtca
     1381 cccagtcgtc gaagagcaag tcgagcattt cattccgaac gacctgtcgc tggatgccgc
     1441 acggcgcatg ctgatcgtca ctgggccgaa catggggggc aagtcgacct acatgcggca
     1501 gactgccgtc atcgcgctgc tcgcatactg cgggctgttc gtcccggcga ccgatgccga
     1561 gatcggcgcg ctcgatgcga tccatacccg catcggtgcc gccgacgatc tcgccggtgg
     1621 gcgttcgacg ttcatggtcg aaatgaccga ggccgcttac atactcaacc gggcgacgcc
     1681 gagcagtctg gtgctgatcg acgaaatcgg ccgcggcacc tcgacgttcg acggactggc
     1741 gctggcatgg gcgatcgcgc gcgagctagc gctcaagaac cgctgcctga cgcttttcgc
     1801 cacgcactac ttcgagttga ctgcgctgcc tgcggagctc gagacgtgcg ccaacgtgca
     1861 tttcgatgcg gtcgagcaca aggacggcat cgttttcctg cacgcggtcg aggaaggtcc
     1921 ggcgaaccag agctacggtc tgcaggtggc acggctagct ggggttacgg ccaccgtgat
     1981 ccgcggcgcg cgcgcgcatc tggcgcgcct cgatcatttc agcgcgcgac gcgacggcga
     2041 tttgttcgcg gctgtcgcgc cggctgccga tccggctccc gcggactcct cgctgcgcga
     2101 tcgattgaag gcgctcgacc cggacgcgat gtcgccgcgc gacgcgcacg agattctcta
     2161 cgagctcaag cgtctggcgg acgatgcgta ggtgcggcct gggatgccgc gccgcctgga
     2221 agctccgcgg cgatcagtgg tggtggccgt gcgcgccgtg cacgtggccg tgcgcgagct
     2281 cttcctcg
//
LOCUS       DATANS010000726         4056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_709433, whole genome shotgun sequence.
ACCESSION   DATANS010000726 DATANS010000000
VERSION     DATANS010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709433"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /gene="metX"
                     /locus_tag="VHT22_10795"
     CDS             <1..606
                     /gene="metX"
                     /locus_tag="VHT22_10795"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296376.1"
                     /note="Catalyzes the conversion of acetyl-CoA and
                     L-homoserine to CoA and O-acetyl-L-homoserine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX3138089.1"
                     /translation="AAAPNLSAQNIAFNEVARAAIMTDPDFHAGHYYDHGVKPLRGLR
                     VARMVGHITYISDEQMGERFGRQLRDGLNYSFAPEFEIESYLRYQGEKFAEYFDANTY
                     LGITKALDYFDPASATGGDLSQALSPARCRFLVIAFTTDWRFSPARSREIVKALVDNR
                     RDVCYAEIVAPHGHDAFLLDTPQYHAIVRAYLARAAEEEAT"
     gene            650..1258
                     /gene="metW"
                     /locus_tag="VHT22_10800"
     CDS             650..1258
                     /gene="metW"
                     /locus_tag="VHT22_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX3138090.1"
                     /translation="MSASYSTRADFSTIAGWIAANARVLDLGCGDGSLLAYLRESRGA
                     FGYGIEIDDAGVLASIGNGVNVIQSDLESGLAGFDDRSFDSVILSQTLQAMRHTEEIV
                     LEMLRVGREAIVTFPNFGHWSHRAQVLRGRMPVSKNLPYQWYDTPNIHLCTVADFDAF
                     CAARRFDVLERVVLHDARRVSMLPNLFGSLAIYRFRHGAAAA"
     gene            1294..2580
                     /locus_tag="VHT22_10805"
     CDS             1294..2580
                     /locus_tag="VHT22_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HEX3138091.1"
                     /translation="MPAMGDSRPPLAKALFNRRMLICIFTGFSSGLPLYILINLLPAW
                     LRTEHVDLKAIGLFALIGLPFTWKFVWSPLVDRYALPLLGRRRGWMLVTQVALIASLP
                     LFGALQPQQDLWTIAYLAAAVAFFAATQDIVLDAYRRELLPDVELGLGNAIHVQAYRI
                     SSLVPGALSLILADRLPWQSVFFITALFMLPGIVNTLLIAEPALSRAAPRTLREAVVE
                     PFHEFITRSGWRQALLVLGFIFLYKLGDSMATALATPFYLDIGFTMSEIGLIAKNAGL
                     WASVFGGLLGGIWMLRIGINRGLWLFGAVQVFSILGFAWLAYVNQPDRLLLAGVIAFE
                     ALGVGLGTAAFTAFIARTTDPRYTATQFALFTSLAAVPRSLINATTGWIVDAIGWFDF
                     YLLCMALALPGMALLLRVAPWNAPTPGAETPAPVKA"
     gene            complement(2604..>4056)
                     /locus_tag="VHT22_10810"
     CDS             complement(2604..>4056)
                     /locus_tag="VHT22_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3138092.1"
                     /translation="SGGWRMRLNLAQALMCRSDLLLLDEPTNHLDLDAVLWLEDWLAR
                     YPGTLLLIAHDRDFLDAVVTEIAHLESNKLRLYTGNYTQFERERALALALQHSAYVKQ
                     QKQIAHLRSYIDRFRAKATKARQAQSRIKALERMELIAAAHVDSPFSFEFPATGIHAR
                     QLLKLDHATLGYGDKAILTDVDWAILAGARIGLLGPNGAGKSTLLRAIAGELKPASGE
                     RLAAQGLSIGYFAQHQVEQLRLDQSALWHLQQIEPGTREQAFRDFLGGFDFRGDRVGA
                     PVREFSGGEKARLTLALLVRQRPNLLLLDEPTNHLDIDMREALTEALQDYTGALIVVA
                     HDRHLLRATADELWLVADGALAPFDGDLDDYRQWVLAGRGREAATVADAAVLTPDRKT
                     QKRVEAESRQRLSELRKPLQQKIERIERRIDLMSTEKRELDEWLATSDAYIEENKERL
                     IASLARRGDLAWELARDEAEWLELHEALERAAG"
BASE COUNT          629 a         1359 c         1309 g          759 t
ORIGIN      
        1 gcggcggcgc cgaacctgtc cgcgcagaac atcgcattca acgaagtcgc tcgcgcggcg
       61 atcatgaccg atcccgactt ccacgccggc cactactacg atcacggcgt caagccgctg
      121 cgcggcttgc gcgtcgcgcg catggtcgga cacatcacct acatctccga cgagcaaatg
      181 ggcgagcgct tcggccggca gttgcgcgac ggcctcaact attcgttcgc gccggaattc
      241 gagatcgaat cgtacctgcg ctaccagggc gagaagttcg ccgagtactt cgatgccaac
      301 acctacctcg gcatcaccaa ggcactcgac tacttcgatc ctgcgtcggc caccggcggc
      361 gacctgtcgc aggcgctttc gccggcgcgc tgccgctttc tggtgatagc gttcaccacc
      421 gactggcgtt tctccccggc acgctcgaga gagatcgtca aggcactggt cgacaaccgg
      481 cgcgacgtct gctacgccga aatcgtcgca ccgcacggac acgacgcctt cctgctggac
      541 acaccgcagt atcacgcgat cgtgcgcgcg tatctcgcac gcgcggctga ggaggaggcc
      601 acgtgagccc gcggcgagac ttttcgatga aaatggaaga ggccgcggtg tgagtgcaag
      661 ctattccacc cgcgccgact tctcgacgat cgccggttgg atcgcggcca acgcccgcgt
      721 gctcgacctg gggtgcggcg acggcagcct gctcgcgtac cttcgcgagt cgcgcggcgc
      781 attcggctac ggcatcgaaa tcgacgacgc cggcgtgctg gcgtcgatcg gcaacggcgt
      841 caatgtcatc cagagcgacc tggaaagcgg gctcgccggc ttcgacgacc ggtcgttcga
      901 cagcgtgatc ctgtcgcaga ccttgcaggc gatgcggcac accgaagaaa tcgtgctcga
      961 gatgctgcgg gtcgggcgcg aagcgatcgt caccttcccg aacttcggcc actggtcgca
     1021 ccgcgcgcag gtgcttcgag gacggatgcc ggtgtcaaag aacctgccct accagtggta
     1081 cgacacaccc aacatacact tgtgcacggt ggccgatttc gacgcgttct gcgcggcccg
     1141 ccgtttcgac gtgctcgaac gcgtcgtgct gcatgacgcg cggcgggtat cgatgctgcc
     1201 gaacctgttc ggctcgctcg cgatctatcg cttccggcac ggcgcggccg ctgcctgaac
     1261 cgccgcgcct gcgcagcgcg gcccttcgct acaatgccgg cgatgggtga ctcgaggccg
     1321 ccgctggcca aagcgctgtt caaccgccgg atgctgattt gcatctttac cggcttcagc
     1381 tcggggctgc cgctctacat tctgatcaac ctgttgccgg cgtggcttcg taccgagcac
     1441 gtcgacctga aggcgatcgg tctgttcgcg ctgatcgggt tgcctttcac gtggaaattc
     1501 gtctggtcgc cgctggtcga tcgctacgcg ctgccgttgc tcggccggcg ccgcggctgg
     1561 atgttggtca cccaggtcgc gctgatcgcg tcgctgccgc tgttcggcgc gctccagcct
     1621 cagcaggatt tatggacgat cgcctacctt gcggcggcgg tggccttttt cgccgcgacg
     1681 caggacatcg tcctcgacgc ctatcgccgc gagctactgc ccgatgtcga gcttggcctg
     1741 ggcaatgcga tccacgtgca ggcctatcgg atatcgagcc tcgttcccgg cgcgctgtcg
     1801 ctgatcctcg ccgaccgcct gccctggcag agcgtctttt tcatcaccgc gctgttcatg
     1861 ctgccgggca ttgtcaacac gctgctgatc gccgagcccg cactgtcgcg cgcggcgccg
     1921 cgcacgttgc gcgaggcggt cgtcgagccg tttcacgaat tcatcacgcg ttccggctgg
     1981 cgccaggcgc ttctggtgct cggcttcatc tttctctaca agctcggcga cagtatggcg
     2041 accgcgctgg cgacgccgtt ctacctggac atcggcttca cgatgtcgga gatcggactg
     2101 atcgccaaga acgccgggct ctgggcgagc gtgttcggcg gcttgctcgg cggcatctgg
     2161 atgctcagga tcggcatcaa tcgcggactc tggctgttcg gcgccgtgca ggttttctcg
     2221 atcctcggct ttgcctggct cgcgtacgtc aatcagccgg accggctgct gctcgccggc
     2281 gtcatcgcct tcgaggcact cggcgtcggg ctcggcaccg ccgcattcac cgcgttcatt
     2341 gcgcgcacca ccgacccgcg ctacacggcg acgcagttcg cgctgttcac gagcctcgcc
     2401 gcggtgccgc gttcgctgat caacgcgacg accgggtgga tcgtcgacgc catcggctgg
     2461 ttcgacttct atctgctgtg catggcgctg gcgctgcccg gcatggcgct gttgttgcgc
     2521 gtggcgccgt ggaatgcgcc gacacccggc gcggaaacgc cggcaccggt caaggcatag
     2581 cgggaccgcg gcgcgaagcg cctttagccg gctgcgcgct cgagcgcttc gtgcagctcg
     2641 agccactccg cttcgtcgcg cgcgagttcc caggccaggt cgccgcgtcg ggcgagcgaa
     2701 gcgatcagcc gctccttgtt ctcttcgatg taggcgtcgg aagtcgccag ccattcatcg
     2761 agctcgcgtt tttcggtcga catgagatcg atcctccgct cgatgcgttc gatcttctgc
     2821 tgcagcggtt tgcgcagctc ggacaggcgc tgcctcgact cggcttcgac gcgcttttgc
     2881 gtcttgcggt cgggcgtcag cactgcggca tcggccaccg tcgccgcctc gcgcccacgt
     2941 ccggccagca cccactgccg gtagtcgtcc agatcgccgt cgaacggcgc aagcgcgcca
     3001 tcggcgacca gccagagctc gtcggcagtg gcgcgcagca ggtgacggtc gtgagcgacg
     3061 acgatcagcg cgccggtgta atcctgcagc gcctcggtca gcgcttcgcg catgtcgatg
     3121 tcgagatgat tggtcggttc gtcgagcagc aggagattcg gccgctggcg gaccagcagc
     3181 gcgagcgtca ggcgcgcctt ttctccgccg gaaaattccc gcaccggtgc gccgacgcgg
     3241 tcgccacgaa aatcaaagcc gccgagaaag tcgcgaaacg cctgctcgcg cgtgcccggc
     3301 tcgatctgct gcaggtgcca cagcgcgctt tgatcaaggc gaagctgctc gacctggtgc
     3361 tgggcaaaat agccgatcga aagtccctgc gccgccaggc gttcgccact agcgggcttg
     3421 agctcgccgg cgatcgcgcg caacagcgtc gacttccccg ccccattggg accgaggaga
     3481 ccgatccgcg caccagccag gatcgcccag tcgacgtcgg tgagtattgc tttgtcgccg
     3541 tagcccagcg tcgcgtgatc gagcttgagc agttgacgcg cgtggatgcc ggtagccgga
     3601 aattcgaacg agaacggact gtcgacgtgc gccgcggcga tcaactccat ccgctcgaga
     3661 gccttgatgc ggctttgcgc ttgccgggcc ttggtcgcct tggcgcggaa gcgatcgatg
     3721 tacgagcgca ggtgcgcgat ctgcttctgc tgcttgacat aggccgaatg ctgcaacgcc
     3781 agagccagtg cgcgttcgcg ctcgaactgg gtgtagttgc cggtatacag gcgcagcttg
     3841 ttgctttcga ggtgggcgat ctcggtgacc acggcgtcga gaaaatcgcg gtcatgcgcg
     3901 atcagcagaa gggtgccggg gtagcgcgcc agccagtcct ccagccagag cacggcatcg
     3961 aggtcgaggt gattggtcgg ttcgtcgagc agcaggagat cggagcggca catcagcgcc
     4021 tgcgcgagat tcaggcgcat ccgccagccg ccggag
//
LOCUS       DATANS010000727         2041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_162749, whole genome shotgun sequence.
ACCESSION   DATANS010000727 DATANS010000000
VERSION     DATANS010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162749"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VHT22_10815"
     CDS             complement(<1..795)
                     /locus_tag="VHT22_10815"
                     /inference="COORDINATES: protein motif:HMM:NF022447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEX3138093.1"
                     /translation="MNPASYFAASYGEARAKFLSAAESAGATVSRHVHPTERGAEGEE
                     LSIDLAMLGEADAPGLLLLISGTHGAEGFCGSGCQVALLDDGDFRADIDRAGVCVLML
                     HALNPYGFSHLRRVNEDNADLNRNFIDFGAPLPVNAAYEEIHSLLLPASWPPPTDSER
                     ELGAWVAAHGRAAYQAAVSGGQYRFADGMFYGGAGPAWSNRVLRGVLREHAASRSSLG
                     WIDFHTGLGPRGHGELIYAGGNVAADIARTKAWWGDAVTSYFDGTST"
     gene            complement(792..1991)
                     /locus_tag="VHT22_10820"
     CDS             complement(792..1991)
                     /locus_tag="VHT22_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEX3138094.1"
                     /translation="MNPVEFVRKEHAALTAVRRDLHAHPELGFEEHRTAEVVARELDA
                     LGVEHHGGIGKTGIVAVIRGAKSESGRAIGLRADMDALPMHEENEFAHVSRFKGKMHG
                     CGHDGHTTMLLGAAAYLSRVRNFDGTVYLIFQPGEEGFAGGKAMIDDGLFERFPADEI
                     YALHNWPGLPPGQIAVRPGPMMAAADRIEIHIEGRGGHGAHPHLTVDPVLVAGHIITA
                     AQSIVARNVSPIDTAVVSVCAMHAGNLAAMSVVPRDARLVGTVRTFNARTQDHIERRL
                     AELVHSIAAAFGATATLKYDRVYPATVNSAREAEFAARVADTLVGPENVVRDLEPSMG
                     SEDFSFMLQRRPGAYARLGQGGAEGGCFLHNTRYDFNDEVIPIGAGYLAALAEAALPL
                     NAKAPTR"
BASE COUNT          329 a          698 c          682 g          332 t
ORIGIN      
        1 cgtcgacgtg ccgtcgaaat acgacgtcac cgcgtcaccc caccaggctt tggtgcgtgc
       61 gatgtcggcg gcaacgttac cgccggcgta gatcagctcg ccgtgtccgc gcggacccag
      121 cccggtgtga aaatcgatcc agcccagtga gctgcggctc gccgcatgct cgcgcagcac
      181 gccgcgcagc acgcggttgc tccaggccgg accggcgccc ccgtagaaca tgccatcggc
      241 gaaacggtac tggccgccgc tgacggccgc ctggtacgcg gcccgcccgt gcgcggcgac
      301 ccaggcgccg agctcccgct cgctgtcggt cggcggcggc caggatgccg gcagcaacag
      361 cgaatggatt tcctcgtaag ctgcattgac cggcagcggc gcgccgaagt cgatgaaatt
      421 gcggttgagg tcggcgttgt cctcgttgac gcgacgcaaa tgcgaaaagc catacggatt
      481 gagcgcatgc agcatgagca cgcaaacgcc ggcgcggtcg atatcggccc gaaagtcgcc
      541 gtcatcgagc agcgccacct ggcagcccga gccgcaaaat ccttcggcgc catgcgtgcc
      601 cgagatcagc agcagcaggc ccggcgcgtc ggcttcgccg agcatcgcca aatcgatcga
      661 caattcctcg ccttcggcgc cgcgctcggt cggatgcacg tgccgggaaa cggtcgcgcc
      721 ggcagactcg gcggcagaaa ggaacttggc ccgcgcttcg ccgtagcttg cggcgaaata
      781 gctggccgga ttcatcgggt cggggccttc gcgttcagcg gcagcgccgc ttcggccagc
      841 gcagcgagat agccggcgcc gatcggtatc acctcgtcgt tgaagtcgta gcgcgtgttg
      901 tgaaggaagc agccgccttc ggcgccaccc tgccccagcc gcgcgtacgc tccgggacgg
      961 cgttgcagca tgaaggaaaa atcctccgaa cccatcgacg gctcgaggtc gcgcacgacg
     1021 ttctcgggcc cgaccagtgt gtcggcgacc cgtgcggcga actccgcctc acgcgcgctg
     1081 ttgacggtcg ccggatagac tcgatcgtat ttgagagtcg cggtcgcgcc aaacgcggca
     1141 gcgatcgaat gcacgagctc cgcaagcctg cgctcgatgt ggtcctgcgt cctggcgttg
     1201 aacgtgcgca cggttccgac caggcgcgcg tcgcgtggca ccacgctcat cgcggcgaga
     1261 ttgccggcgt gcattgcgca gacgctgacg acggcggtat cgatcgggct cacattgcgc
     1321 gcgacaatcg actgcgctgc ggtgatgatg tgcccggcca cgaggaccgg atcgacggtc
     1381 aggtgcggat gcgcgccgtg gccgccgcgg ccctcgatat ggatctcgat gcgatcggcc
     1441 gccgccatca tcggccccgg acgcacggcg atctgtccgg gcggaagtcc cggccagttg
     1501 tgcagcgcgt agatctcgtc ggcgggaaag cgttcgaaca agccatcgtc gatcatcgcc
     1561 ttgccgccgg caaacccttc ttcgccgggc tggaagatca ggtagacggt accgtcgaag
     1621 ttgcgcacgc gggacagata tgctgcggcc ccgagcagca tcgtcgtgtg cccgtcgtgt
     1681 ccgcagccgt gcatcttccc cttgaagcgg gagacgtgcg cgaactcgtt ctcctcgtgc
     1741 atcggcagcg catccatgtc ggcgcgcaag ccgatcgcgc ggccgctttc ggacttcgca
     1801 ccccggatca ccgcgacgat gccggtcttg cctatgccgc cgtgatgctc gacgccgagc
     1861 gcgtcgagct cgcgcgcgac gacctcggcg gtgcgatgct cctcgaagcc gagctccgga
     1921 tgcgcgtgca gatcgcgccg cactgcggtc agcgcggcgt gttccttgcg gacgaactcg
     1981 acgggattca tgcgcttgct ccggcggccc gcgctgcagc cttgtggcga ctcattgcgc
     2041 t
//
LOCUS       DATANS010000728         3285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_120309, whole genome shotgun sequence.
ACCESSION   DATANS010000728 DATANS010000000
VERSION     DATANS010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120309"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VHT22_10825"
     CDS             complement(<1..966)
                     /locus_tag="VHT22_10825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX3138095.1"
                     /translation="MSRRRRVAVTGIGLVTPVGNDVSTTWTALLEGRSGVAPISIFDA
                     SGFSTRIAAEVKDFDAASLGAERKLLKFANRSHRFALGAAEQAMRDAGIAPTAATATR
                     WGCSVGTGMMGVAYEDLSAVQRHSAAAGELHADQLVSDPTAADPIVFCRSQSTAGLAL
                     LTRRFGIHGYATSVHTACASGGQAIGTALKLIRRGSVDYALAGGFDSMISPVGLGGFC
                     LLSAVSADNDAPQSASRPFDATRNGFVLGEGAGFVVLEEWEAARRRGAHIYAELAGDG
                     NSLSSYRITDSPPDGDGPIQSMRAALADAGAAPEDVDYITAHGTST"
     gene            complement(963..1811)
                     /locus_tag="VHT22_10830"
     CDS             complement(963..1811)
                     /locus_tag="VHT22_10830"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138096.1"
                     /translation="MSDHFAAYSFVDRITEFAPGAHARGWYAVPRDIAEFPSCLVAEA
                     VGQLAAWVAMDKLGYCGRPVAALATETLFEGSVAPGDLLELDVDIDDCDDEVIAYGGR
                     AKVNGRQVIELRHCLGPMLPVEELDSPDALRERFALLRGRGAAAGGFRGVAPVPVTVD
                     ARVAAKSLHATLMVPEHAAFFGDHFPRRPVFPATLLLDLQMRLAIELANSLGESRGAA
                     AMTPSRMTNVKMRTFITPGQQVGINVELLPAPDDSATARIALSAHVGDRAVATARVEL
                     VDRVLA"
     gene            complement(1831..2259)
                     /locus_tag="VHT22_10835"
     CDS             complement(1831..2259)
                     /locus_tag="VHT22_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX3138097.1"
                     /translation="MAQNEISKEEILAVYPKVAETMADALGCDVEEVKLDVSLIEGLD
                     AESIDFLDMVFRLERAFKIKIPRGKFVENARGDLPEAEFEQKGIVTEKGLAQLKSYLS
                     EIPAERFKTPMKVADIPRLFTPETFCKLVISAQREAQAAT"
     gene            complement(2265..3005)
                     /locus_tag="VHT22_10840"
     CDS             complement(2265..3005)
                     /locus_tag="VHT22_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003253959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3138098.1"
                     /translation="MAGKVAIVTGGSRGIGRAVVELLADEGADVTFFYRDNAQAAAEV
                     IAAGGKAGRAIGAEQVDVCDAKACADAVERVAARTERIDILVNNAGIIRDNQLAALGD
                     DDIRVVLDTNIGGVFNTTRAVIPHMIVQRSGKIINLSSVSAEKGGRGQTNYAASKGAI
                     NAFTRSLAVELAPRKITVNAVAPGVIETEMSQDVRDLAGDQVKSRILLKRYGKPEEVA
                     YAVWFLASRYADYITGQVVAVDGGFKME"
     gene            complement(3031..>3285)
                     /locus_tag="VHT22_10845"
     CDS             complement(3031..>3285)
                     /locus_tag="VHT22_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003391580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3138099.1"
                     /translation="TLAQELGPKNIRVNAVSAGVVDTEALSHFPNREALLADFARRTP
                     AGPVLTPEEVAGAVYLLCLPEAAMITGHTLVVDGGFCISG"
BASE COUNT          549 a         1116 c         1057 g          563 t
ORIGIN      
        1 ggtcgaggtg ccgtgcgcag tgatgtagtc gacgtcctcc ggcgcggcgc cggcgtcggc
       61 aagggctgcg cgcatcgact ggatcggccc gtcgccatcc ggcggcgagt cggtgatgcg
      121 gtacgacgaa agcgaattgc cgtctccggc aagctcggcg tagatatgcg cgccgcgccg
      181 gcgcgccgct tcccattcct cgaggacgac aaatccggct ccttcgccga gcacgaagcc
      241 gttgcgcgtc gcgtcgaatg gacggctggc gctctgcggc gcatcgttgt cggccgaaac
      301 cgccgacaga agacagaagc cgcccagtcc gaccgggctg atcatcgaat cgaagcctcc
      361 cgcaagtgcg taatcgacgc tgccccggcg tatcagcttg agtgccgtgc cgatcgcctg
      421 gccccccgac gcgcaggcgg tgtggacaga ggtcgcataa ccgtggatgc cgaagcggcg
      481 ggtcagcaag gccagtcccg ccgtcgactg actgcgacag aacacgatcg gatcggccgc
      541 ggtcggatcg ctgaccagtt ggtcggcgtg cagctcgccg gcggccgccg aatgccgctg
      601 cacggccgaa agatcctcgt acgcgacgcc catcatgccg gtcccgaccg agcagcccca
      661 gcgcgttgcg gtcgccgccg tcggcgcaat gccggcgtct cgcatcgcct gctcggccgc
      721 accgagcgca aatcgatgcg agcgattggc gaacttgagg agtttccgct cggcaccgag
      781 cgaggccgca tcgaaatcct tgacctcggc ggcgatccgc gtcgaaaagc cgctggcgtc
      841 gaaaatgctg atcggcgcaa cgccgctgcg gccttcgagc aacgcggtcc acgtcgtgga
      901 cacatcgttg cccaccggcg tgaccagtcc gatccccgtg accgcgaccc tgcgccgccg
      961 gctcatgcga gcaccctgtc gaccagttcg acacgcgccg tcgccaccgc gcgatcgccg
     1021 acatgggcgc tcaacgcgat ccttgcggtc gcggagtcat cgggtgccgg cagcaactcg
     1081 acgttgatgc cgacttgctg gcccggcgtg atgaacgtgc gcatcttgac gttggtcatg
     1141 cgcgagggcg tcattgccgc ggcgcctcgc gactcgccga gcgaattggc gagctcgatg
     1201 gcaagcctca tttgcagatc gagcagcaag gtcgcaggaa acacaggacg gcggggaaag
     1261 tgatcgccga agaacgccgc atgctcgggc accatcagcg tggcgtgcag cgacttggcc
     1321 gccacgcgcg catcgacggt gacaggcacc ggcgccaccc cgcgaaagcc gccggccgcc
     1381 gcacctcgcc cgcgcagcag cgcgaagcgt tcgcgcaatg catccggcga atcgagctcc
     1441 tcgaccggca gcatcggacc caggcagtgc cggagctcga tgacctgcct gccgttgacc
     1501 ttcgcccggc cgccataggc gatgacttca tcgtcgcaat cgtcgatgtc gacgtcgagc
     1561 tccagcaggt cgccgggcgc gacgctcccc tcgaacaagg tttcggtcgc cagcgccgca
     1621 accggtcttc cgcagtagcc gagcttgtcc atcgcaaccc acgcggcgag ctgtccgacg
     1681 gcttcggcga ccaggcacga cggaaactca gcgatatcgc gcgggaccgc gtaccagccg
     1741 cgagcatgtg cgccgggcgc gaattcggta atccggtcga caaaggagta tgcggcgaaa
     1801 tgatcgctca tgcggcgctt cggcccctcg tcaggtcgcg gcctgggctt cgcgctgcgc
     1861 gctgatcacc agcttgcaga atgtctccgg cgtgaacagg cgcggaatat cggcgacttt
     1921 catcggcgtc ttgaatcgct cggccggaat ctcggacaga taggatttga gctgcgccag
     1981 tcccttttcg gtgacgatcc ctttctgctc gaactcggct tccggcaggt cgccgcgcgc
     2041 attctcgaca aacttgccgc gcggaatctt gatcttgaac gcgcgctcga gacggaacac
     2101 catgtcgaga aaatcgatcg attcggcgtc gagcccctcg atcagcgaca cgtcgagctt
     2161 gacctcttcg acgtcgcaac cgagcgcgtc ggccatcgtt tcggcgacct tcgggtacac
     2221 cgccaggatt tcttccttgc ttatctcatt ttgcgccatg ctgttcactc cattttgaag
     2281 ccgccgtcga cggcgactac ctgtccggta atgtagtcgg cgtagcgtga ggccagaaac
     2341 cagaccgcat acgcgacttc ctcgggcttg ccgtaacgct tgagcaggat gcgcgatttc
     2401 acttggtcgc cggcgagatc gcgcacatcc tgagacattt cggtttcgat cacgccgggt
     2461 gcgacggcat tgaccgtgat cttccgcggc gcgagctcga ccgccaatga gcgcgtgaag
     2521 gcgttgatcg cgcccttgct cgccgcgtag ttggtctggc cacgcccgcc cttctcggcg
     2581 ctgaccgagg aaaggttgat gatcttgccc gagcgctgca cgatcatgtg tggaatcacc
     2641 gcccgggtgg tattgaacac gccgccgatg ttggtgtcga ggacgacgcg gatgtcgtcg
     2701 tcgccgagcg ccgcgagctg attgtcccgg atgatccccg cgttgttgac caggatgtcg
     2761 atgcgctcgg tgcgcgcggc aacccgctcg acggcgtcgg cgcaggcctt ggcgtcgcag
     2821 acgtcgacct gctcggcgcc gattgctctg cccgccttgc cgccggcggc gatgacttcc
     2881 gccgcggcct gcgcgttgtc gcgatagaag aaggtgacgt ccgcaccttc gtcggcgaga
     2941 agctcgacga ccgcgcggcc gatgccgcga ctgccgccgg tgacgatcgc gactttgccg
     3001 gccagcccgg aatcgatttt catcgccggc tcatcccgaa atgcagaatc cgccatcgac
     3061 gaccagtgta tgaccggtga tcatcgccgc ctcgggcagg cacagcaggt agacggctcc
     3121 ggccacctcc tccggcgtca gcaccggccc tgcgggcgtc cgtcgagcga aatcggccag
     3181 cagcgcctca cgattcggga agtggctcaa cgcttcggta tcgacgacgc cggcgctgac
     3241 ggcgttgacc cggatgttct tcggcccgag ctcctgtgcc agcgt
//
LOCUS       DATANS010000729         1549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_499057, whole genome shotgun sequence.
ACCESSION   DATANS010000729 DATANS010000000
VERSION     DATANS010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499057"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VHT22_10850"
     CDS             <1..454
                     /locus_tag="VHT22_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX3138100.1"
                     /translation="CQLRPESRGSLKIRSADPGVPPEIRINYLATETDRRAFIDGIRI
                     LRKILAAPALKAYAVAEVDPGSKVQSDDELLDFCRRTGSTVYHPTSTCRMGSDALAVV
                     DQRLRVRGIEGLRVVDASVMPDLMSGNTNAPTIMIAEKASDMILEDAR"
     gene            complement(592..>1549)
                     /gene="hemE"
                     /locus_tag="VHT22_10855"
     CDS             complement(592..>1549)
                     /gene="hemE"
                     /locus_tag="VHT22_10855"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX3138101.1"
                     /translation="GRYLPEYRELRAKAGGFLDLCFTPEYAAEVTLQPIRRFNFDAAI
                     IFSDILVIPFALGRSVRFEAGEGPRLDPLDTPEQVTGLATQADFGKLEPVYEALRRVK
                     RELAPDIALIGFCGAPWTVATYMVAGQGTPDQAPARMMAYRHPEAFAEIIDVLVENSI
                     QYLLGQLKAGADALQIFDTWAGVLPPREFERWSIEPAKRIVAGVRAQVPDAKIIGFPR
                     GAGALLPAYVEATGVNAVSIDWAAEPSLIRERVQSRVAVQGNLDPLALIAGGAALDRA
                     VDDVLANYAQGPLIFNLGHGIQPETPIAHVEQMLQRVRAYRG"
BASE COUNT          269 a          505 c          521 g          254 t
ORIGIN      
        1 ctgccagctg cgtccggaga gccgcggttc gctgaagatc aggagcgcgg acccgggcgt
       61 gccgccggaa atccgcatca actatctggc gaccgagacc gatcgccgcg ccttcatcga
      121 cggcatccgt atcctgcgca agatcctggc agcccctgcg ctgaaggctt atgcggtggc
      181 agaggtcgat ccgggctcga aagtgcagag cgacgatgag ttgctggact tctgccgccg
      241 taccggcagc acggtctatc acccgacctc gacctgccgc atgggcagcg atgcgctcgc
      301 cgtcgtcgac cagcgcttgc gcgtgcgcgg catcgaaggt ctgcgcgtgg tcgatgcgtc
      361 tgttatgccg gacctgatgt cgggcaatac caacgcgccg accatcatga tcgcggagaa
      421 ggcgtcggac atgattctgg aggacgcgcg gtaggcgacg acgccagcgg cgaacaccga
      481 tgtcatcatc cgcgaaggcg gatgatccag tacgccgtga cgtctcgatt cctatttctg
      541 ccggcgatta ctggatgccc gccttcgcgg gcatgacgaa ggcgtccgtc gtcaccccct
      601 atacgcccgc acccgctgca gcatctgctc gacatgcgcg atcggggttt ccggctggat
      661 gccgtggccg aggttgaaga tcagcggccc ttgggcgtag ttcgccagca cgtcgtcgac
      721 ggcgcgatcc agcgccgcgc cacccgcgat cagcgccagc gggtcgaggt tgccctgcac
      781 cgcgacgcgg ctttgcacgc gctcgcggat caaggacggc tcggccgccc agtcgatgct
      841 gacggcgttg acaccggttg cttccacata ggccggcaac agcgcgccgg cgccgcgggg
      901 aaagccgatg atcttggcgt cgggcacttg cgcgcggacg ccggcgacga tgcgtttcgc
      961 gggctcgatc gaccagcgct cgaattcgcg cggcggcaat acgccggccc aggtatcgaa
     1021 gatctgcaac gcgtccgcgc cggccttgag ctggccgagc agatactgga tcgagttttc
     1081 caccagcacg tcgatgattt cagcaaaggc ctcgggatgg cgataggcca tcatccgcgc
     1141 cggcgcctga tcgggcgtgc cctgtcccgc aaccatgtag gtcgcgaccg tccacggcgc
     1201 gccgcagaag ccgatcagcg cgatgtcagg cgcgagttcg cgcttcacgc ggcgcagtgc
     1261 ttcatagacc ggctcgagct tgccgaaatc ggcctgcgtg gccaaacccg tcacctgctc
     1321 cggcgtatcc aggggatcga gccgcgggcc ttcgccggcc tcgaagcgca cggaacggcc
     1381 gagcgcgaag ggaatgacga gaatgtcgga aaagatgatc gcggcgtcga agttgaagcg
     1441 gcggatcggc tgcagcgtga cttcggcggc atattccggc gtgaagcaca ggtcgagaaa
     1501 gccgccggcc ttggcgcgca gttcgcgata ttccggcaga tagcggccg
//
LOCUS       DATANS010000730         2477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_35946, whole genome shotgun sequence.
ACCESSION   DATANS010000730 DATANS010000000
VERSION     DATANS010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35946"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VHT22_10860"
     CDS             complement(<1..484)
                     /locus_tag="VHT22_10860"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633363.1"
                     /note="catalyzes the formation of porphobilinogen from
                     5-aminolevulinate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX3138102.1"
                     /translation="MNFTGKFPERRPRRMRRDDFSRRLMRENRLSSDDLIYPAFVLEG
                     SRREEPVLSLPGISRKSIDLLLGDAERCLALGVPAIALFPVIPAAQKSLDAAAAWDPK
                     GLLPQTVRALKARFPTLGVITDIALDPYTSHGQDGLLDDTGYVANDETVAALTRQALC
                     H"
     gene            complement(500..643)
                     /locus_tag="VHT22_10865"
     CDS             complement(500..643)
                     /locus_tag="VHT22_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138103.1"
                     /translation="MTGRAIKEFHVQRDDLFDRVQPDKTDLLPATMRASYDALAATSR
                     NQG"
     gene            complement(743..1411)
                     /gene="yihA"
                     /locus_tag="VHT22_10870"
     CDS             complement(743..1411)
                     /gene="yihA"
                     /locus_tag="VHT22_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003822057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX3138104.1"
                     /translation="MKHPFQNAEFLTTAADWHQLPVDGAPEVAFAGRSNAGKSSAINA
                     LAQRTRLAFVSKTPGRTQNINFFRLRSGALLADLPGYGYAEVPAAIRRHWQHFIARYL
                     AERVPLTGLVLVMDARRAMTDLDRQMLDWFLPSLRPTHILLTKADKFTVAEQRRILAG
                     VQRELAQDYAAYADRISAQLFSATRKLGLADAEQAVGSWLAPFAVEAAMLPAQKERPR
                     HQGE"
     gene            1555..2199
                     /locus_tag="VHT22_10875"
     CDS             1555..2199
                     /locus_tag="VHT22_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX3138105.1"
                     /translation="MKCALLLTAVAAAVSLGTPASAEEAAGKPDLAKAQQIVNQVCSA
                     CHGADGNSPSPANPNLAGQQADYIALQLVHFKTGIRNNPVMAGMAATLTPDDMRSLGA
                     YFAQQKPKGLAAKDADLAAAGQKLFRGGDAAGGVPACAACHAPDGAGIPSRYPRLGGQ
                     YADYTLAQLRAFKAGERGMDKEGKDINGRIMAQIAGRMNERAIAAAAQYAAGLH"
     gene            2260..>2477
                     /locus_tag="VHT22_10880"
     CDS             2260..>2477
                     /locus_tag="VHT22_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX3138106.1"
                     /translation="MTASIAALFVYPVKGCRGIALSSALVTERGLEHDREWMVVDIAG
                     RFVTQRTEPRLASITTAITATSLILAMAG"
BASE COUNT          438 a          822 c          802 g          415 t
ORIGIN      
        1 catggcacaa cgcctgacgt gtcagtgccg ccaccgtctc gtcgttggcg acatagccgg
       61 tgtcgtccag caagccatcc tgcccatgac tcgtataagg gtcgagcgcg atgtcggtga
      121 tcacgcccag cgtcggaaat cgcgctttca gcgcgcgcac ggtttgcggc aacagcccct
      181 tcggatccca tgcggcggcc gcatcgagcg atttctgcgc agccggaatc accggaaaca
      241 acgcgatcgc cggtaccccg agggcgaggc agcgctcggc gtcgcccaag agcagatcga
      301 tgctcttgcg ggagattccc ggcagcgaaa gcacgggctc ctcgcgccgg cttccctcga
      361 ggacgaacgc cgggtaaatc aggtcatcgc tggacagtcg attctcgcgc atcagacggc
      421 gcgaaaaatc gtcgcgacgc attcttcgcg gccggcgctc gggaaatttt ccggtaaagt
      481 tcatcgacat agcgagcagt cagccctggt tgcgcgaagt cgctgcgagc gcatcgtagc
      541 tcgcgcgcat cgttgcgggc aacaagtccg ttttgtcggg ctgcacgcga tcgaacaaat
      601 cgtcgcgctg gacgtggaac tcttttatcg cgcggccggt catatcccgc agacgatagc
      661 atcgtctccc gctttcctcc ctgagcgggt gcccttcggt tgcgtaaccc taccaaccga
      721 agggcgttct tcgggccccg tctcactccc cttgatgtcg gggcctttct ttttgtgccg
      781 gcagcatcgc ggcctccacc gcaaatggcg caagccagct ccccaccgcc tgctccgcat
      841 cggcaagacc aagctttcgc gtcgccgaaa acagctgcgc gctgattcga tccgcatatg
      901 ccgcgtagtc ctgcgctagt tcgcgctgca cgccggcgag aatgcgtcgc tgttcggcga
      961 cggtaaactt gtcggccttg gtcaagagga tgtgggtcgg acgcaacgag ggaaggaacc
     1021 agtcgagcat ttgccggtcc aggtcggtca tcgcccgccg cgcgtccatc accaatacca
     1081 gcccggtgag cggcacgcgc tcggccagat agcgcgcgat aaaatgctgc cagtgccgcc
     1141 gaatcgcggc gggcacctcg gcgtaaccgt acccgggcag atcggcgagc agggcgcccg
     1201 agcgcaggcg aaagaagttg atgttctgcg tgcgtcccgg cgtcttgctg acgaacgcca
     1261 gtcgcgttcg ctgcgccagc gcgttgatcg ccgaggactt gccggcgttc gatcgcccgg
     1321 cgaatgcgac ctcgggcgcg ccgtcgacag gcagctggtg ccaatcggcg gccgtcgtca
     1381 ggaactccgc gttttggaat ggatgcttca ccccgctcgt cccggcagcg tcgtttcggc
     1441 cggccgcgag ggattcggta caatacgcgg tcggcttgcg tcggatgcgt gcgcaagcga
     1501 cgcccgatgc gcggactgga ccgccgggcc gccgaatcga gggagcgtca aacaatgaaa
     1561 tgcgcgttgt tgctgacggc ggtagccgcc gcggtttcgc tcggcacacc ggcaagcgcc
     1621 gaggaggccg ccggaaagcc ggatctcgcc aaagcgcagc agatcgtcaa ccaggtttgc
     1681 tcggcctgcc atggcgccga tggtaacagc ccgagcccgg ccaatcccaa cctcgccgga
     1741 cagcaggccg actacatcgc cctgcagctc gtccatttca aaaccgggat ccgcaacaat
     1801 ccggtgatgg ccggaatggc cgcgacgctg acacctgacg acatgcgatc gctgggcgcc
     1861 tatttcgcgc agcagaaacc gaaggggttg gcggcaaagg acgccgatct cgccgccgcg
     1921 ggtcaaaagc tgtttcgcgg cggcgacgcc gccggcggcg tgcctgcgtg cgccgcatgc
     1981 cacgcaccgg atggcgcagg cattccttcg cgctatccgc gcctcggcgg ccagtatgcc
     2041 gactacacgc tggcgcagct gcgcgcgttc aaggcgggcg agcgcggcat ggacaaggaa
     2101 ggcaaggaca tcaacggaag gatcatggcg cagatcgccg gcaggatgaa cgagcgggcg
     2161 atcgcggcgg cggcccagta cgccgccggc ttgcactgag cgtgcaacgg ccggacagtc
     2221 aaaggcggcg atcgcgccgc cttttttatc ggcgctgaca tgactgcctc gatcgcggcg
     2281 ctgttcgtct atccggtcaa gggctgccgc ggcatcgcgc tgtcttcggc gctggtcacc
     2341 gaacgcgggc tcgagcacga ccgcgagtgg atggtcgtcg atatcgccgg gcgattcgtt
     2401 acccaacgca ccgagccccg gctggcatcg atcacgaccg caatcaccgc cacctcgctg
     2461 atactggcga tggccgg
//
LOCUS       DATANS010000731         1715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_540360, whole genome shotgun sequence.
ACCESSION   DATANS010000731 DATANS010000000
VERSION     DATANS010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540360"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..767
                     /locus_tag="VHT22_10885"
     CDS             183..767
                     /locus_tag="VHT22_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138107.1"
                     /translation="MRSIARMGLIAAVAVLGGCQILTYEGDRFSSTVYDEIPVRPFHF
                     PTKLTYPDYTMFGFPMYSEDPRADLRLDSISCRRAADGGLVVLASVRNMGAEDIYRVP
                     FSGDMAAFRVAATVTTKAGAQERFEAVRYLRLNVASNAVLTLNSTSVPAADVTRIDVV
                     ADPDRVVPDPIRDNNVLSWNGAIDGANPQCDVHR"
     gene            938..1687
                     /locus_tag="VHT22_10890"
     CDS             938..1687
                     /locus_tag="VHT22_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX3138108.1"
                     /translation="MKILVPVKRVVDFNVKVRVKPDGSGVDLANVKMSMNPFDEIAVE
                     EAVRLKEKGIASEIVAVSCGMASCQETLRTALALGADRAILVETAVELQPLAVAKLLR
                     ALVAKEAPQLVILGKQAIDDDANQTGQMLAALLGWAQGTFASKVDVADDGVTVRREID
                     GGMETIAMKLPAIITTDLRLNEPRYATLPNIMKAKKKPLESTTPDALGVDVAPRLTTL
                     KVVEPAKRKGGGKVADVKELVAKLRNEAKVI"
BASE COUNT          320 a          571 c          558 g          266 t
ORIGIN      
        1 gctgcgatta gggcctgaat aaggggcttg acagccacgg gcggccgccg ccgccggcag
       61 gagttgacct ccgggccccg gcaccgcatt atgacgctgc cataagcatg actgcggctt
      121 tccggccgcc gtctatttgg accgctgctg cagcgcagga caacggtccc agggagaaga
      181 acatgcgctc gatcgccagg atgggactca tcgccgccgt cgcagtgctg ggcggctgcc
      241 aaatcttgac ctacgaggga gatcgtttct cgagcaccgt ttacgacgag attccggtgc
      301 gtccgttcca cttcccgacc aagctcacct accccgacta cacgatgttc ggcttcccga
      361 tgtacagcga ggatcctcgc gccgacctga ggctcgactc gatcagctgc cgccgcgccg
      421 ccgacggcgg cctggtcgtg ctggccagcg tgcgcaacat gggcgccgaa gacatctacc
      481 gcgtcccgtt ttccggcgac atggcggcct ttcgtgtcgc cgcgacggtg acgaccaaag
      541 ccggcgcgca ggagcgcttc gaagcagtgc gctacctccg gctcaacgtc gccagcaatg
      601 cggtgctcac gctcaactca acgtcggtac cggctgcgga tgtgacccgg atcgacgtcg
      661 tcgccgatcc ggatcgcgtc gtgcccgatc cgatacgcga caacaacgtg ctgagctgga
      721 acggcgcgat cgacggggcc aatccgcaat gcgacgtcca ccgctgacgg tttagcttcg
      781 aatagccgcg gcgctgcgcc ggcgcggctc acgcgagtcc ctcaccgatc cggcgccggg
      841 cgccgggcgg tgccgcaggc aattcacgct ataatcacgc caccgcgcgg caccgtcgga
      901 cgcggcttca tcgcgcattc ccagcctttc gcaagcaatg aaaatcctgg tcccggtcaa
      961 gcgagtggtc gacttcaacg tcaaggtgcg ggtcaagcct gacggcagcg gcgtcgacct
     1021 cgccaacgtc aagatgtcga tgaacccgtt cgacgaaatc gccgtcgagg aagccgtccg
     1081 gctgaaggaa aaaggcatcg ccagcgaaat cgtcgccgtg tcgtgcggca tggcgtcatg
     1141 ccaggagacg ctgcgcaccg cactggcgct gggcgctgac cgcgcgatcc tggtcgagac
     1201 tgccgtcgag ttgcagccgc tggccgtcgc caagctgttg cgcgcgctgg tcgcgaagga
     1261 agcgccgcag ctggtgatcc tgggcaagca ggcgatcgac gacgacgcca accagaccgg
     1321 tcagatgctc gcggcgctcc tcggctgggc gcaggggacg ttcgcctcga aggtcgatgt
     1381 cgccgacgac ggcgtaaccg tcaggcgcga aatcgacggc ggcatggaga cgatcgcgat
     1441 gaagctgccg gcgatcatca cgaccgacct gcgcttgaac gagccgcgct acgcgaccct
     1501 gcccaacatc atgaaggcga agaaaaagcc gctggagtcg acgacgccgg atgcgctggg
     1561 cgtcgatgtc gcgccgcggc ttacgacgct caaggtcgtc gagccggcga agcgcaaggg
     1621 cggcggcaag gtggccgacg tcaaggagct ggtcgccaag ctccgcaacg aagccaaggt
     1681 aatctagacc tcatgatcct cgtcgtcgcc gagca
//
LOCUS       DATANS010000732         1605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_373153, whole genome shotgun sequence.
ACCESSION   DATANS010000732 DATANS010000000
VERSION     DATANS010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373153"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VHT22_10895"
     CDS             <1..471
                     /locus_tag="VHT22_10895"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEX3138109.1"
                     /translation="SMEAVYQAELRELRSAETQACAIVDEIWVTLLNGPLADRVSDYV
                     ATLRRRAAEHEGSSHRNQVMRALVHEASRIAENCTTNVRDVILVATLQHVVHYLIASY
                     GTLAAHAKSLGRMDQAERFHAFAESDRQFDLDLSSLAKTEVNPQAVGQRVGTAS"
     gene            complement(447..1124)
                     /locus_tag="VHT22_10900"
     CDS             complement(447..1124)
                     /locus_tag="VHT22_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX3138110.1"
                     /translation="MIKVLCGAVLALVSGIAAAQSQPTATHTLYLIRHGAYVADRNAD
                     PQLGPGLTALGIAQARLIAARLNGAGVKFDSMTSSPMQRARDTAAVMHETLATLPVAQ
                     SPLLRECMPPVFESVEAEAAERAACAKQLDQAFAEFFTPTKNANRNDILVAHGNVIRY
                     LVMKALKVDTRAWLGMSVPHASLTVIQVYADGSTKVLAVGDSGHIPSPLLSWGDANDR
                     QLAVPTR"
     gene            complement(1121..1504)
                     /locus_tag="VHT22_10905"
     CDS             complement(1121..1504)
                     /locus_tag="VHT22_10905"
                     /inference="COORDINATES: protein motif:HMM:NF045340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138111.1"
                     /translation="MQEIIVLLAAVVVSAAAWGQVTKLESTVKYRPTFVDSDFGDIAK
                     YVGELTSRTFELETGVCAQVTAHWDKPMTSDEFYRAFLGIARALGYVVVEDGLVTKIQ
                     LAADTPKDPTPPCRRYPVRNQVQNR"
BASE COUNT          290 a          510 c          514 g          291 t
ORIGIN      
        1 agcatggaag cggtctacca ggccgagctg cgcgagctgc gcagcgcaga gactcaggcc
       61 tgcgcaatcg tcgatgaaat ctgggtgacg ttgctcaacg gtccgctggc ggatcgcgtc
      121 agcgactacg ttgccacgct acgccgacgg gccgccgagc acgaaggcag cagtcatcgc
      181 aatcaggtga tgcgcgcgct ggtgcacgag gcgagtcgga tcgcggagaa ctgtacgacg
      241 aacgttcgcg acgtcattct ggtcgcgacg ctccagcacg tagtgcacta cctgatcgcg
      301 agctacggca cgctcgccgc gcatgcgaaa tcgctgggtc gcatggatca ggccgaacgt
      361 ttccatgctt tcgcggagag cgatcgtcag ttcgacctgg atctcagctc gctggcgaaa
      421 accgaggtca atccgcaggc ggtgggtcag cgcgtcggca ccgcgagctg acggtcgttc
      481 gcatcgcccc aactgagcag cgggctcgga atgtgcccgc tgtcgccgac tgcgagcacc
      541 ttggtcgagc catctgcata cacctggatc actgtcaggc tcgcgtgcgg cacggacatg
      601 cccagccagg cccgcgtgtc taccttcagc gccttcatca ccaggtagcg gatgacgttg
      661 ccatgggcga cgagaatatc gttgcgattc gcattcttgg tgggcgtgaa gaattccgcg
      721 aatgcctggt ccagttgttt cgcgcacgct gcccgttcgg cggcttcagc ctcgacagat
      781 tcgaacaccg gcggcatgca ctcgcgcagc aacggtgact gcgcgaccgg cagagtcgca
      841 agcgtttcgt gcatcactgc cgcggtatcg cgcgcccgtt gcatgggact cgacgtcatc
      901 gagtcgaact tgacgccggc gccattcaag cgcgcggcga tcaggcgcgc ctgcgcgatg
      961 ccgagagcag taagccccgg gccgagctgc ggatcggcat tgcgatcggc gacatacgcg
     1021 ccgtgacgaa tcaagtagag cgtgtgcgtg gccgtcggct gcgattgagc cgctgcgatt
     1081 ccgctgacca gcgcgagcac cgcgccgcag agcactttga tcatcgattc tgcacctgat
     1141 tgcgcacggg atagcggcgg cacggcggcg tcggatcctt gggagtgtcg gcggcgagct
     1201 gaatcttcgt gaccaggccg tcttccacga cgacgtaacc gagcgcgcgg gcgatcccaa
     1261 ggaacgcgcg gtagaactca tccgacgtca tcggcttgtc ccagtgcgcg gtcacctgcg
     1321 cgcacacgcc tgtctcgagc tcgaacgtgc ggctggtcag ctcaccgacg tacttcgcga
     1381 tatcgccgaa atcagagtcg acgaacgtcg gccggtactt gaccgtgctc tccagcttcg
     1441 tgacttgccc ccacgcagcg gccgatacga ccaccgcggc gagcagcacg atgatttctt
     1501 gcatgcgcct ggccatcact ggatcttctt cacgatccgg tcggtcgtgc cgaactcgcg
     1561 gtaaatgtaa tgcgtgacgc ggccgtccgc atctttggcg aagat
//
LOCUS       DATANS010000733         1995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_163170, whole genome shotgun sequence.
ACCESSION   DATANS010000733 DATANS010000000
VERSION     DATANS010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163170"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VHT22_10910"
     CDS             <1..491
                     /locus_tag="VHT22_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HEX3138112.1"
                     /translation="DVPYANDEERKIAEGVGGYARPMPPEWMARQAGIISERCKTVDI
                     LITTALIPGRPAPKLIKAETVAAMKPGAIVVDLAVEQGGNVEGSELGKIVVKNGVKLV
                     GLPNLPALVPADASALYARNVLNFLALSLNAKSGEFAVPADDEIVKATLVCANGQVVP
                     RT"
     gene            536..853
                     /locus_tag="VHT22_10915"
     CDS             536..853
                     /locus_tag="VHT22_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX3138113.1"
                     /translation="MTAGVDPIVINLIVFVLAIFVGYHVVWNVTPALHTPLMSLTNAI
                     SSIILVGAMLAAGAADLDWAGWLGVVAVTLVSINVFGGFLVTQRMLGMFRKKERSTGA
                     TKE"
     gene            856..>1995
                     /locus_tag="VHT22_10920"
     CDS             856..>1995
                     /locus_tag="VHT22_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX3138114.1"
                     /translation="MSANQLALWYLVAAVSFILALKGLSSPHTARRGNVYGMAGMAIA
                     LLVTLAVVYSHSRHVLPILGGMAVGGALGAIVARRVQMTQMPELVAAMHSLVGLAAVL
                     IAIAAVKNPQAMGLDFPITFGHKLELFIGTFIGAITFSGSVIAFGKLSGRIRSAPVVF
                     PGQHYVNLAIGVAMIGFGIWFCASPPAAEWPPFLIMTALAFLLGFLLIIPIGGADMPV
                     VISMLNSYSGWAAAGIGFSLDNPMLIIAGSLVGSSGAILSYIMCRAMNRSFFSVILGG
                     FGGEIADAAAGGEKKPVKSGSPDDAAFLLSNADTVIIVPGYGLAVARAQHAVKELAAK
                     LTAKGVNVKYAIHPVAGRMPGHMNVLLAEAEVPYDQVFEMEDINSE"
BASE COUNT          339 a          620 c          669 g          367 t
ORIGIN      
        1 tcgatgtgcc gtatgccaac gacgaggagc gcaagatcgc cgagggtgtc ggcggctacg
       61 cgcgcccgat gccgcccgag tggatggcac ggcaggcggg catcatcagc gaacgctgca
      121 agaccgtcga cattctgatc accactgcgc tgataccggg acggccggcg ccgaagctga
      181 taaaggccga gacggtcgcc gcgatgaagc ccggcgcgat cgtcgtcgat ctcgcggtcg
      241 agcagggcgg caacgtcgaa ggctcggagc tcggcaagat cgtcgtcaag aacggcgtca
      301 agctggtggg gctgcccaac ctgccggcgc tggtgccggc agacgccagc gcgctctatg
      361 cgcggaatgt gctcaacttt cttgccttgt cgctcaacgc caagagcggc gaattcgcgg
      421 tgcccgccga cgacgagatc gtcaaggcca cgctggtgtg cgcgaacggg caggtcgtac
      481 ccagaacctg acagcgggct tcccgtccgc gggaatgttg cgccggagag caaccatgac
      541 agcaggcgtt gatccgatcg taatcaacct gatcgtgttc gtgctggcga tcttcgtcgg
      601 ctaccacgtc gtctggaacg tgacgccggc gctgcatacg ccactgatgt cgctgaccaa
      661 tgcgatctcg agcatcatcc tggtcggcgc gatgctcgcg gcgggagcgg ccgatctcga
      721 ctgggccggc tggctcggcg tcgtcgcggt cacgctggtg tcgatcaacg tgttcggcgg
      781 cttcctggtc acgcagcgca tgctcggcat gttcagaaag aaagaacgca gcaccggcgc
      841 gaccaaggaa tagcgatgag cgccaaccaa ctcgcgctgt ggtacctggt cgccgcggtg
      901 agcttcatcc ttgcgctgaa gggcctttcg agcccgcaca ccgcgcggcg tggcaacgtg
      961 tacggcatgg ccggcatggc gattgcgctg ctggtcacgc tggctgtggt ttactcgcac
     1021 agcaggcacg tgttgccgat cctaggcggc atggcggtgg gcggcgcgct cggggcaatc
     1081 gtcgcgcggc gcgtgcagat gacgcagatg cccgagctcg tcgcggcaat gcattcgctg
     1141 gtcggtctcg ccgccgttct gattgccatc gcggcggtga agaacccgca ggcgatgggg
     1201 ctcgacttcc cgatcacttt cggccacaag ctcgagcttt tcatcggcac cttcatcggc
     1261 gcgattacct tttccggatc ggtgatcgcc ttcggcaagc tctccggccg catacgcagc
     1321 gcgccggtcg tgttcccggg ccagcattat gtcaatctcg ccatcggtgt tgcgatgatc
     1381 ggcttcggga tctggttctg cgcgtcgcct ccggcagcgg agtggccgcc attcctgatc
     1441 atgacggcgc tcgcgttcct gctcggcttt ctgctgatca ttccgatcgg cggggccgac
     1501 atgccggtcg tgatctcgat gctgaacagc tactcgggct gggcggcggc aggcatcgga
     1561 ttctcgctcg ataacccgat gctgatcatc gccggatcgc tggtcggctc ctcgggtgcg
     1621 attctttcgt acatcatgtg ccgcgcgatg aaccgttcgt tcttcagcgt gattctcggc
     1681 ggcttcggcg gcgaaatcgc cgacgcggca gcgggcgggg agaaaaagcc ggtcaagagc
     1741 ggcagtcccg acgatgcggc attcctgctg tcgaatgcgg acacggtgat catcgtcccc
     1801 ggctatggac tcgcggtcgc gcgagcacag cacgccgtca aggagctggc ggcgaagctg
     1861 acggcgaagg gcgtcaacgt caaatacgcg atccacccgg tcgccggacg catgccgggg
     1921 cacatgaacg tgctgctcgc tgaagcggag gtcccatacg accaggtgtt cgaaatggag
     1981 gacatcaaca gcgag
//
LOCUS       DATANS010000734         1532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_289976, whole genome shotgun sequence.
ACCESSION   DATANS010000734 DATANS010000000
VERSION     DATANS010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289976"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VHT22_10925"
     CDS             <1..281
                     /locus_tag="VHT22_10925"
                     /inference="COORDINATES: protein motif:HMM:NF037640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX3138115.1"
                     /translation="FGLIADDTLVCRCEEVTAGEIRAAVAAWGASAGAVKGVTRCGMG
                     YCQGRICGSLLEELTARAAGCAREDVGGLSVRPPLKPVRVGELADLAE"
     gene            307..1248
                     /locus_tag="VHT22_10930"
     CDS             307..1248
                     /locus_tag="VHT22_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX3138116.1"
                     /translation="MSTALAWSSAARAQQAYPAKPIRLILPFPPGGSTDIVARLIGQK
                     LTESWGQPVLIENRPGAGGNIAAETAARAAPDGYTLFQVNVANAIGATLYPKLPYDLI
                     TSFAPVIQLATTPYVLLAHPTVPAKNTAELIALAKARPGQLNYASAGGGSATHLSGEL
                     LKSMAGVNIVHVPYKGTGPAVTALLSGEVDLYFATVPAALPLVETKKLRALGVTSARR
                     SPLMRDVPAIAEAGLKGYETSTWHGILAPAATPADIVVKLNAEIARTLAQPAVRERLV
                     GQGLDPVGGTPEQFGAYLKTEISKWAVVVKASGARPE"
     gene            1340..>1532
                     /locus_tag="VHT22_10935"
     CDS             1340..>1532
                     /locus_tag="VHT22_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138117.1"
                     /translation="MRLPYALKIVLENLLRQRARGEDRDSDVAALLAWPEGGAQREIR
                     FRPARVMMDDTAGIPSLGDL"
BASE COUNT          253 a          510 c          543 g          226 t
ORIGIN      
        1 tattcggact gatcgccgac gacacgctgg tgtgccgttg cgaggaggtc accgcgggcg
       61 aaatccgcgc cgcggttgcc gcatggggcg cgtcggcggg cgccgtcaaa ggcgtcacgc
      121 gctgcggcat gggctactgc cagggacgca tctgcggcag tctgctcgag gaattgacag
      181 cgcgcgccgc cggctgcgcg cgcgaagacg tcggtggcct gagcgtgcgg ccgccgctga
      241 aaccggtgcg cgtaggcgaa cttgccgacc tcgcggaata atcgggccgt tgccatggtg
      301 ctcgcgatga gcaccgcgct cgcgtggtct tctgcggccc gcgcgcagca ggcttatccg
      361 gcgaagccga tccggctgat cctgccgttc ccgccgggcg gcagcaccga catcgttgcg
      421 cggctgatcg gtcaaaaact gacggagagc tggggccagc ccgtgttgat cgagaacaga
      481 cccggcgccg gcggcaatat cgcggcggaa acggcggcgc gcgcggcgcc tgacggttac
      541 acgctgttcc aggtcaacgt cgccaacgcg atcggcgcga cgctgtatcc gaagctcccc
      601 tacgatctga tcacgagctt tgcgccggtg atacagctcg cgacgacgcc gtacgtgctg
      661 ctcgcgcatc cgaccgtgcc ggcgaaaaac accgccgaac tcatcgcgct cgccaaggcg
      721 cggccgggac agcttaatta cgcatccgcg ggcggcggca gcgcgacgca tttgtccggc
      781 gagctcttga agtcgatggc gggcgtgaac atcgtgcacg tgccctacaa aggcacgggc
      841 cccgctgtca ccgcgctgct gtcgggagaa gtcgatctgt acttcgccac ggtgccggcg
      901 gcgctgccgc tggttgagac gaagaaattg cgtgcgctcg gcgtgacgag cgctcgccgc
      961 tcgccgctga tgcgcgacgt gccggcgatt gccgaagccg gcctcaaagg ctacgaaacc
     1021 agcacctggc acggcatcct ggcgccggca gcaacacctg cggacatcgt cgtgaaactg
     1081 aacgccgaga tcgcgcgcac ccttgcccag cccgcggtca gggagcggct ggtcgggcag
     1141 gggctggatc cggtcggcgg cacgcccgag cagttcggcg cgtacctcaa aacggaaatc
     1201 agcaagtggg cagtcgtcgt gaaggcgtcg ggagcgcgcc ctgagtaacg gcgcaacgcg
     1261 cccgcaggac tgggccgagc tcgcggtcgg cgcccgcacg gttcattact gcagtgtcgc
     1321 ggctgccggc gcgcgcgcga tgcgcctgcc ttacgcgctc aaaatcgtgc tcgaaaacct
     1381 gctgcggcaa cgcgccagag gcgaggatcg cgacagcgac gtcgccgcgc tgctcgcgtg
     1441 gccggagggc ggcgcgcagc gcgaaatccg gttccgtccg gcgcgcgtga tgatggacga
     1501 caccgcgggc ataccgtcgc ttggcgatct ag
//
LOCUS       DATANS010000735         3954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_582568, whole genome shotgun sequence.
ACCESSION   DATANS010000735 DATANS010000000
VERSION     DATANS010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582568"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VHT22_10940"
     CDS             <1..433
                     /locus_tag="VHT22_10940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3138118.1"
                     /translation="DGVLQVQAGFANSSHPLIKGKLSFIDNTADMTTGTIKLKAEFPN
                     VDKALWPGQFVNVVMTLYDQKDAVVAPSAAVQNGPNGQYVYVVKPDMTVELRNIKVTR
                     TEGDETIVASGLQPGDQVVTVGQLRLAPGTRVSVGKGTEPS"
     gene            430..3540
                     /locus_tag="VHT22_10945"
     CDS             430..3540
                     /locus_tag="VHT22_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3138119.1"
                     /translation="MNIAEIFVRRPVMTILVMTGILIFGVIAYRLLPVNALPNVDFPT
                     IQVQAQLPGASPDTMASAVATPLEKQFSTIAGIDSMTSASTDGIATITMQFSLDRSID
                     AAAQDVQSAIAAAARSLPSSMPNPPTQRKVNPADAPILNIVLSSATMPLSTVDDYAEN
                     LLAQQISTITGVAQVAVYGSQQYAVRIQLDPNALATRGLALTDVEQAVSNSNVNLPTG
                     TLYGRDKATSVQATGQLMNAAAYAPMIVAYRNGAPVRLNELGRVFDSVQNDKIAAWYN
                     GTRAIMLAVQRQPGTNTIEIVDAVRKILPTFESQLPPAIKLNVFYDRSVSIRAAVHDV
                     QMSLLLALVLVVAVIFVFLRSVSATIIPSLALPLSIIGTFALMYAFNYSLDNLSLMAL
                     TLCVGFVVDDAIVMLENITRHIEMGKSRMQATLDGSKEIGFTIVSMTLSLVAVFIPVM
                     FMGGILGRLLREFAVTIIVAVLISGIVSLTLTPMLCSRMLVDEHKRKHGRVYAFSERV
                     FEAVLDGYRRSLALALRHQGLVLAAFAGVAICTGVLFATMSKGFLPADDNGQILVFTE
                     AAQDVSFQAMSAMQRQVAEIVKENPNVEGAMSSVGAGGPSASLNVGRIFVALTPRNTR
                     PNADTVVQQLRGPLSRVSGIKAYVQNIPAIRIGGQITKSSYQYTLQGTDTSQLYQWAP
                     RIEEKLQSLPALVGVTSDLQIAKPQVTVEIDRNKASALGVSAQEIENTLYDAYGQRQV
                     STIYAPTNQYWVVMELQPRYQTDASVLSLLYVRSASGSLVPLNSVATLRPTVGPLSIN
                     HLGQLPAVTISFDLKPGVAIGDAIKEIDKAMAEMRPPATIIGVYQGTAQAFQSSLRGM
                     GILLVLSVFVIYLVLGILYESFIRPLTILSGLPTAGFGALLTLLLFGVELNMYAFVGI
                     IMLVGIVKKNAIMMIDFAIEAQRKESKPPAEAIYQACLTRFRPIMMTTMAALMGSLPI
                     ALGLGAGGETRRPLGLAVVGGLVVSQALTLYITPVIYLYFERFQQWLATRRATHAEEP
                     AHAAAD"
     regulatory      3583..3688
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3733..>3954
                     /locus_tag="VHT22_10950"
     CDS             3733..>3954
                     /locus_tag="VHT22_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236773.1"
                     /note="catalyzes the formation of
                     4-amino-2-methyl-5-phosphomethylpyrimidine from
                     5-amino-1-(5-phospho-D-ribosyl)imidazole and
                     S-adenosyl-L-methionine in thiamine biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX3138120.1"
                     /translation="MNANDQFFARDAHVDDAAVQPLPNSRKVHVEGSRPDLRVPMREI
                     AQSDTPASFGAEVNPPLAVYDTSGPYTDPT"
BASE COUNT          685 a         1300 c         1258 g          711 t
ORIGIN      
        1 cgacggcgtg ctgcaggtcc aggcagggtt cgccaactcg tcccatccgc tgatcaaggg
       61 caagctgtcg ttcatcgaca ataccgccga catgacgacc gggacgatca agctcaaggc
      121 ggaatttccg aatgtcgaca aggcgctgtg gcccgggcag ttcgtcaacg tcgtgatgac
      181 cctgtacgac cagaaggacg ccgtcgtcgc gccatcggcg gcggtgcaga acggacccaa
      241 cggccagtat gtctacgtgg tcaagcccga catgaccgtc gagttgcgca acatcaaggt
      301 cacgcggacc gagggtgacg agacgatcgt cgccagcggc ctgcagccgg gcgaccaggt
      361 cgtgaccgta ggccagctgc ggctggcgcc cggcacacgg gtcagcgtcg gcaagggaac
      421 ggagccgtca tgaacatcgc cgagatcttc gtccggcgtc cggtgatgac gatactggtg
      481 atgaccggca tcctgatctt cggcgtcatc gcgtaccgcc tgttgccggt caacgcgctg
      541 cccaacgtcg atttccccac gatccaggtg caggcgcagc tgcccggcgc gagccccgac
      601 acgatggcct cggcggtcgc cacgccgctg gaaaagcagt tctcgaccat cgccggcatc
      661 gactcgatga cctcggcttc gaccgacggc atcgcgacga tcacgatgca gttttcgctc
      721 gatcgcagca tcgatgcggc ggcgcaggac gtgcagtcgg cgatcgccgc cgcggcgcgc
      781 tcgctgccct cgtcgatgcc caaccctccg acgcagcgca aggtcaaccc ggccgatgcg
      841 ccgatcctca atatcgtcct gtcgtcggcg acgatgccgc tgtcgacggt cgacgactat
      901 gccgagaatc tgctcgcgca gcagatctcg acgatcaccg gcgtcgcgca ggtcgccgtg
      961 tacgggtcgc agcagtacgc ggtgcgcatc cagcttgacc cgaatgccct tgccacgcgc
     1021 ggcctggcgc tgaccgacgt cgagcaggcg gtttccaaca gcaacgtcaa tctgccgacc
     1081 ggaacgcttt acgggcgcga caaggcgact tcggtgcagg cgaccgggca gctgatgaat
     1141 gcggcggcct acgcgccgat gatcgtcgcc tatcgcaacg gcgctccggt ccggctgaac
     1201 gagctcggcc gcgtattcga cagcgtgcag aacgacaaga ttgccgcctg gtacaacgga
     1261 acgcgcgcga tcatgctcgc ggtgcagcgc cagccgggaa ccaacacgat cgagatcgtt
     1321 gacgcggtca gaaagattct tccgaccttc gaatcgcaat tgccgccggc gatcaagctc
     1381 aacgtcttct atgaccgctc ggtgtcgatc cgcgccgcgg tgcacgacgt gcagatgtcg
     1441 ctgctgctgg cgctggttct ggtcgtcgcc gtgatcttcg ttttcctgcg cagcgtgtcg
     1501 gcgacgatca tccccagcct ggcgctgccg ctgtcgatca tcggcacttt cgcgctgatg
     1561 tacgcattca actacagcct cgacaacctg tcgttgatgg cgctgacgct gtgcgtcggc
     1621 ttcgtcgtcg acgacgcgat cgtgatgctg gaaaacatca cccgccacat cgaaatgggc
     1681 aagtcgcgga tgcaggcgac gctcgatggg tcgaaggaaa tcggcttcac gatcgtctcg
     1741 atgaccctgt cgctggtcgc ggtgttcatc ccggtgatgt tcatgggcgg cattctcggc
     1801 cgcctgttgc gggaattcgc cgtcacgatc atcgtcgccg tgctgatctc gggcatcgtc
     1861 tcgctgacgc tgacgccgat gctgtgcagc cgcatgctgg tcgacgagca caagaggaag
     1921 cacggccgcg tttatgcgtt ctccgaacgc gtgttcgagg cggtgctcga cggctacagg
     1981 cgctcgctgg cgctggctct gcgccaccag ggactggtgc tggcggcatt cgccggcgtc
     2041 gccatctgca ccggcgtgct tttcgccacg atgtcgaagg gattcctgcc cgccgacgac
     2101 aacggccaga tcctggtatt caccgaggcc gcgcaggacg tctcgttcca ggcgatgtcc
     2161 gccatgcagc ggcaggtcgc cgaaatcgtc aaggagaatc cgaacgtcga gggggcaatg
     2221 tcgtcggtcg gtgccggcgg ccccagcgcc tcgctcaacg tcgggcgaat cttcgtcgcc
     2281 ctgacgccgc gcaacactcg ccccaatgcc gacaccgtcg tgcagcagct gcgcgggccg
     2341 ttgtcgcggg taagcggcat caaggcatac gtgcagaaca tcccggcgat tcgcatcggc
     2401 ggacagatca ccaagagctc gtatcagtac acgctgcagg gcaccgacac gtcgcagctg
     2461 tatcagtggg cgccgagaat cgaggagaag ctgcaatccc ttcccgcgct ggtcggtgtg
     2521 accagcgacc tgcagatcgc caagccgcag gtcactgtcg agatcgaccg caacaaggcg
     2581 tcggcgctgg gcgtctcggc acaggagatt gagaacacgc tgtacgacgc ctacgggcag
     2641 cgccaggtgt cgacgatcta cgcaccgacc aatcagtact gggtcgtgat ggagttgcag
     2701 cctcgctacc agaccgatgc ttcggtcctg tcgctgctct atgtgcgctc ggcgagcgga
     2761 tcgctggtgc cgctcaacag cgtggcgacg ttgcggccga cggtcgggcc gctttcgatc
     2821 aaccatctgg gccagttgcc cgccgtgacg atatcgttcg atctgaagcc cggcgtggcg
     2881 atcggcgacg ccatcaagga gatcgacaag gcgatggcgg aaatgcgccc tcccgccacg
     2941 atcatcggcg tctaccaggg caccgcgcag gcgttccagt cgtcgttgcg cggcatgggc
     3001 atcctcctgg tgctctcggt tttcgtcatc tatctggtgc tgggcatcct gtacgagagc
     3061 ttcattcgtc cgctgacgat tctttccggg ttgccgaccg ccggcttcgg cgcgctgctg
     3121 acgctgctgc tgttcggcgt cgagctcaac atgtacgcct tcgtcggcat catcatgctt
     3181 gtcggcatcg tcaagaagaa cgccatcatg atgatcgact tcgcgatcga ggcgcagcgc
     3241 aaggagtcga agccccccgc ggaggcgatc taccaggcgt gcttgacgcg gttccggccg
     3301 atcatgatga cgacgatggc agcattgatg ggcagcttgc cgatcgcgct ggggctcggg
     3361 gcgggtggcg aaacgcggcg gccgctgggc ctggcggtcg tcggcggcct cgtggtttcg
     3421 caggcgctga cgctctacat cacgccggtg atttacctgt acttcgagcg gttccagcag
     3481 tggctcgcca cgcgacgcgc gacgcacgcg gaagagcccg cgcacgctgc cgcggactga
     3541 ttcctcgcgc ttgccgccga acccgctttg gcgtaacctc gattcgaacg ctaggggtcc
     3601 cctcgtgcca ttgaaagcga ggaggtgaga tcaaaccctt ggtacctgtg cggataatgc
     3661 cggcgcaggg aagcgtgctt cactctcgta tcgctcccct gcccgctcga tccgccgccg
     3721 cagaggagca ccatgaacgc caacgaccag tttttcgccc gcgacgcgca cgtcgacgac
     3781 gcggctgtcc agccgctgcc taattcccgc aaggtccacg ttgagggctc gcggccggac
     3841 cttcgcgtgc cgatgcgcga gatcgcgcaa tccgacacgc cggcgtcgtt cggggccgaa
     3901 gtcaatccgc cgctcgccgt ctacgacacg tccggtccgt acaccgaccc gacg
//
LOCUS       DATANS010000736         1856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_77843, whole genome shotgun sequence.
ACCESSION   DATANS010000736 DATANS010000000
VERSION     DATANS010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77843"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VHT22_10955"
     CDS             complement(<1..719)
                     /locus_tag="VHT22_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEX3138121.1"
                     /translation="METLLFSPLTIRGLTLRNRIVLSPMLTYSATNGYTNDTHLAHLA
                     KFAVGGAGLVFVESTKIDPRGCSTPRDLGLWKDEFVPGMKRIVDLVKSYGAACGIQLG
                     HSGRKARRSVPWEGRVPLRDVPGVDHGEPWELIAPSAIKHDANYESPREMTHADIAEM
                     VDAWSQGARRAVEAGFDVLEIHGAHGYLIHQFLSATANQRTDQYGGTLENRMRFAVEV
                     VRGVRRYWPAEKPLFLRVSAV"
     gene            complement(740..>1856)
                     /locus_tag="VHT22_10960"
     CDS             complement(740..>1856)
                     /locus_tag="VHT22_10960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HEX3138122.1"
                     /translation="IVAGTMEVELVPQGILVERCRAGGAGIPAFYSPTSVGTDIANGK
                     ETREFGGKQYVLEHAINVDYAFLRGYRADRLGNVQFRGGSQNFNPSFAKAARVAIVEV
                     DEIVEAGVIPPELIDLPGIFVSRVVKTTQVVDGKAWRRPERRPSDQPRLYNGKPALTR
                     AGIAKRAAALVKDGTYVNLGVGIPTMVSNYLQKRDVILHAENGVLGYGEMVTGDDVDP
                     DIYNAAGQFVSLKPGASFFDSVTSFEMARGGRIDTVILGAYEVDQTGSVANWSTSDAK
                     RGGIGGAMDLLSGKGDLIIVMEHADSKGRPKVKKKCTYPLTGKGCVNYVVTDLALLHW
                     QDGKFILEEVAPGFTAEEVMKLGEMEMTPAANVENMA"
BASE COUNT          306 a          616 c          575 g          359 t
ORIGIN      
        1 tccaccgccg acacgcgcag gaacagcggc ttctccgcag gccagtaacg ccgcacgccg
       61 cgcacgactt caaccgcgaa gcgcatccgg ttttccagcg taccgccgta ctgatcggtg
      121 cgctgattgg cggtggccga caggaactgg tggatgaggt agccgtgcgc gccgtgtatc
      181 tccagcacgt cgaagcctgc ctcgacggcg cggcgggcgc cttgcgacca cgcatcgacc
      241 atctccgcga tgtccgcgtg cgtcatctcg cgcggggatt catagttcgc gtcgtgcttg
      301 atggcgctgg gggcgatgag ttcccacggt tcgccgtgat cgacgcccgg cacgtcgcgc
      361 agcggcacgc gcccctccca cggcacggag cggcgtgcct tgcggccgga atgtccgagc
      421 tggatgccgc aggccgcgcc atagcttttt accaggtcga cgatgcgctt catgcccgga
      481 acgaactcgt ctttccacag ccccagatcg cgcggcgtcg agcagccgcg cggatctatc
      541 ttggtcgact cgacgaagac gagcccggcg ccgcccaccg cgaatttcgc caagtgcgcg
      601 agatgggtgt cgttggtata gccgttggtc gcggaatacg tgagcatcgg ggagagcacg
      661 atgcgattgc gcagcgtgag gccgcgaata gtcagcggcg aaaacagcag cgtttccata
      721 cgcgtgcctg cctgcacatt cacgccatgt tttctacgtt tgccgccggc gtcatttcca
      781 tttcgccaag cttcatcact tcttcggcag tgaatcccgg cgcgacttct tccaggatga
      841 actttccatc ctgccagtgc agcagcgcca gatcggtgac cacgtaattc acgcagcctt
      901 tgccggtcag cggataggtg cattttttct tcactttcgg ccggcctttg ctatcggcgt
      961 gttccatcac gatgatgaga tcgcctttgc ccgacagcag gtccatcgca ccgccgatgc
     1021 cgccgcgctt tgcgtcggac gtgctccagt tggcgacgct gccggtctga tccacttcgt
     1081 acgcgccgag gattacggtg tcgatgcgtc caccgcgcgc catctcgaac gacgtcacgc
     1141 tatcgaagaa cgacgcgccc ggtttcaacg acacgaactg gccggcggcg ttatagatgt
     1201 cgggatcgac gtcgtcgccc gtcaccatct caccatagcc gagcacgcca ttctcggcgt
     1261 gcaatatcac gtcgcgtttt tgcagatagt tcgaaaccat cgtcgggatg cccacgccga
     1321 gattgacgta agtgccgtcc ttgacgagcg cggcggcgcg cttggcgatg cccgcgcgcg
     1381 tgagcgcggg cttgccgttg tacaaccgcg gttgatcgga tggccgccgc tcgggccgcc
     1441 gccacgcctt gccgtcgacg acctgcgtcg tcttgaccac tcgcgacacg aagattcccg
     1501 gcaggtcgat gagctcgggc ggaatgacgc cggcctcgac gatttcatcg acttcgacga
     1561 tagccacgcg cgccgccttg gcgaaactcg ggttgaagtt ctggctgccg ccgcggaact
     1621 ggacattgcc caggcgatcc gcgcgatagc cgcgcaggaa tgcgtagtcg acgttgatcg
     1681 catgctcgag cacgtattgc ttgccgccga actcgcgcgt ctccttgccg ttcgcgatgt
     1741 cggtgcccac gctggtcggg gaatagaacg ccgggatgcc cgcgccgccc gcgcggcagc
     1801 gctcgaccag gatgccctgc ggcacgagtt cgacttccat ggtgccggcg acgatc
//
LOCUS       DATANS010000737         5431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_332338, whole genome shotgun sequence.
ACCESSION   DATANS010000737 DATANS010000000
VERSION     DATANS010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332338"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..891
                     /gene="mutM"
                     /locus_tag="VHT22_10965"
     CDS             76..891
                     /gene="mutM"
                     /locus_tag="VHT22_10965"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX3138123.1"
                     /translation="MPELPEVETTRRGVAPHVERQKVTAVRIYDRRLRWPVPRDLPRR
                     LVGRVVDRVDRRSKYLLFRLGADTLIVHFGMTGSLRVYGVAPARRTHDHIDLEFANGV
                     VLRYHDPRRFGAMLWSPAAAGEHPLLAALGPEPFSAGFDAAYLFASTRKRSTAIKLAL
                     MDNRLVVGAGNIYANEALFRAGIRPTRAANRVSREQLGRLVTAVRETLAAAIAKGGST
                     LRDYVDSDGAPGYFQLEYNVYGREGQPCRVCGEPIRTTRQAGRATYYCQGCQT"
     gene            964..2928
                     /locus_tag="VHT22_10970"
     CDS             964..2928
                     /locus_tag="VHT22_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEX3138124.1"
                     /translation="MTQSKDLAARFEAYSDWRRRLSTGVSRLHEWLSEQELADGQADL
                     TVQRLLERLHQDKLVVAFVAEFSRGKSELINAIFFADFGQRLLPSAAGRTTMCPTELL
                     YDSTREPSIRLLPIETRARDGSVTEFKNYADEWVTITLDMSSADRMAEALSQVSQTKR
                     VSVPLARKYGLYDDGDPLAPLSAADAGVDVPCWRHAIINFPHPLLQQGLVILDTPGLN
                     AIGTEPELTLNLLPNAHAILFILAADAGVTKTDIDVWTKHLVGDTAASREGRLVILNK
                     IDGLWDDLRTAHEIEGEIARQVRTSAQLLMIPPAQVFPVSAQKALLAKVNGDDVLLAR
                     SRLPQLEAALSRELIPAKRRIVGSATQTEIRELVAGTRTILDSRIAGIREQLTELQNL
                     RGKNQDVVEHMMERIQQEKNLFERGMQRYTALRTVFTQQTNSLYESIGLETLRATAAD
                     TRERIEKSPFTKGVRSAMGDFFAKIYGSLDQSARQTAETHEMMQAMYQRFAKEHGIEP
                     FTPPPFSMLKYQKEVERLERAYNVHFNTLWNMVSKAKFTLMKRFFETIAIRVKHVFDI
                     ANRDLESWLKAVMAPLETQVREHHLQLRRRLESVKRIHRASDELEDRIDELENAEVAA
                     KAQIGDLAREVAAIDAICEQSEPLPLAANA"
     gene            complement(2948..3217)
                     /locus_tag="VHT22_10975"
     CDS             complement(2948..3217)
                     /locus_tag="VHT22_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEX3138125.1"
                     /translation="MALIITDECINCDVCEPECPNGAIAQGPEIYVIDPGKCTECVGH
                     FDTPQCVEVCPVDCIPVNPDFVESKDQLLLKYRGLMAAKAGNVPA"
     gene            complement(3217..3726)
                     /gene="coaD"
                     /locus_tag="VHT22_10980"
     CDS             complement(3217..3726)
                     /gene="coaD"
                     /locus_tag="VHT22_10980"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX3138126.1"
                     /translation="MLKIVYPGTFDPFTKGHEDIVRRAAKLFDRVIVAIADSESKRPM
                     FTADERIAMAREVLAPYANVEVAGFSGLLMDFVHSVGATTILRGLRAVSDFEYEFQMA
                     GMNRNLHPEVETLFLTPAEQYLFMSSTIVREIARFGGDVGKFVPPAVVARLKAKADEE
                     RAAQSRERS"
     gene            complement(3761..4312)
                     /gene="rsmD"
                     /locus_tag="VHT22_10985"
     CDS             complement(3761..4312)
                     /gene="rsmD"
                     /locus_tag="VHT22_10985"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX3138127.1"
                     /translation="MPQAHQIRIIGGRWRGRRIRFPAVADIRPTPDRVRETLFNWLGQ
                     DLDGASTLDPFAGSGALSFEALSRGATLAVAVDRKPDLVRALHAAAEMLGATLEAHCA
                     DAQAFLARETRLFDVVFLDPPFAAPQWEALLPAASARVAADGALYVESSAPVVAPAGF
                     AIVRGDKAGQVHYHLLRRLPAAA"
     gene            4370..5308
                     /gene="ftsY"
                     /locus_tag="VHT22_10990"
     CDS             4370..5308
                     /gene="ftsY"
                     /locus_tag="VHT22_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX3138128.1"
                     /translation="MLDMFKRKPDGGERSWSDRLKSGLGLSRERISRPLTALFGREAL
                     SEEAVDELRSALLGADVGIPATEHLLDELVARWKRAGADLDTRGVLKSALVELIAPLE
                     QPLVIGSARPFVIMLAGVNGAGKTTSIGKLAKYLQGQGLSVLLAAGDTFRAAAREQLE
                     TWGARNNIQVIAQHGGDPAAVMFDAISAARARRIDVVLADTAGRLPTQMPLMEELKKI
                     RRVIAKAQEGAPHETLLVLDANTGQNALAQVAAFDAALGLTGLVLTKLDGTAKGGVVA
                     AIAKTRPLPLKLIGVGEGIDDLRPFRAAEFADALIG"
BASE COUNT          987 a         1808 c         1770 g          866 t
ORIGIN      
        1 cctgcgcctc gccgggcctc cgttgcatct tagggtatat tggtccgagc aacaaacgcc
       61 actcgacgcc cccagatgcc cgaacttccg gaagttgaaa cgacgcgtcg cggggtcgcg
      121 cctcacgtag aacggcagaa agtgacggcg gtgaggatct acgaccgcag gttacgctgg
      181 ccggttccac gcgatctgcc ccgccggctg gtcggtcgcg tcgtcgaccg ggtcgaccgg
      241 cgcagcaaat acctgctttt ccgcctgggc gccgacacgc tgatcgtcca tttcgggatg
      301 accggaagcc ttcgcgtcta cggcgtcgca ccggcgcggc gcacccacga tcacatcgac
      361 ctggaattcg ccaacggagt cgtccttcgt taccacgacc cgcgccgttt cggcgcgatg
      421 ctgtggtcgc ccgccgccgc tggcgagcat ccgctgctcg ccgcgctcgg acccgagccg
      481 ttctcggccg gtttcgatgc ggcttacctg ttcgcttcca cccgcaagcg cagcacggcg
      541 atcaagctcg cactgatgga caaccgcctg gtggtcggcg cgggcaacat ctacgccaac
      601 gaggcattgt ttcgcgccgg catacggcca acgcgcgccg ccaaccgcgt ttcgcgagag
      661 cagctgggcc gcctggtcac cgcggtgagg gaaacgctgg ccgcggcgat cgccaaaggc
      721 ggcagcaccc ttcgcgatta tgtcgatagc gatggcgcgc ccggttactt tcagctcgaa
      781 tacaacgtat acggccgcga aggccagccc tgccgtgttt gcggcgagcc gattcgcacg
      841 acccggcagg cagggcgagc gacctattat tgccagggtt gccagacctg accaggctta
      901 ggtgcttgtt ttggcgcgct ttatgctaca gtcgctgcga gccagcggga ggacgcggcg
      961 aaaatgacgc aatcgaagga tctggctgcc cgatttgaag cctacagcga ctggcgacgg
     1021 cggctgtcga ccggcgtttc acgccttcat gaatggctgt ccgagcagga actggccgat
     1081 ggccaggccg acctgaccgt ccagcgcctg ctcgagcggc tgcaccagga caagctggtc
     1141 gtcgcttttg tcgccgagtt ttcccgcggc aaatcggagc tgatcaacgc gatcttcttt
     1201 gccgatttcg gtcagcgcct gctgccctcg gccgccggcc gcacgacgat gtgtccgacc
     1261 gagctcctct acgactcgac gcgggagccg tcgatccgcc tgttgccgat cgagacccgg
     1321 gcccgcgacg gatcggtcac cgaattcaag aactacgcgg acgaatgggt gacgatcacg
     1381 ctcgacatgt cgtcggcgga tcggatggcc gaagcgctct cgcaagtgtc gcagaccaag
     1441 cgcgtctcgg tgccgcttgc gcgcaagtac ggcctctacg atgacggcga cccgctggcg
     1501 ccgctgtcgg ccgctgacgc cggcgtcgac gttccctgct ggcgccacgc gatcatcaac
     1561 tttccgcatc cgttgctgca acaggggctg gtgatactcg acacgcccgg cctgaacgcc
     1621 atcggcaccg agcccgagct cacgctcaac ctgctgccga atgcacacgc gatcctgttc
     1681 atcctcgccg ccgacgccgg cgtgaccaag accgacatcg acgtatggac caagcacctc
     1741 gtcggggaca ccgccgcgag ccgcgaaggc cggctggtga tcttgaacaa gatcgacgga
     1801 ctgtgggacg accttcgcac cgcgcacgag atcgaggggg aaatagcgcg tcaggtccgc
     1861 accagcgcgc aactgctgat gatcccgccg gcgcaggttt ttccggtgtc ggcgcagaag
     1921 gcgctgctgg cgaaggtcaa cggtgacgac gtgctgctgg ccaggagccg cctgccgcag
     1981 ctcgaggcgg cgttgtccag ggagctgatt cctgccaagc ggcgcatcgt aggctcggcg
     2041 acgcagaccg agatccgcga gctcgtggcg ggcacgcgga caatactcga cagccgcatc
     2101 gccggcatcc gcgagcagct gaccgagctg cagaacctgc gcggaaagaa ccaggatgtg
     2161 gtcgagcaca tgatggagcg catccagcag gaaaagaact tgttcgagcg aggcatgcag
     2221 cgttacacgg ctctgcgcac ggtattcacg cagcagacca acagcctgta cgaaagcatc
     2281 gggctggaga cgctgcgcgc cacggctgcc gacacgcggg agcgaatcga gaaaagcccg
     2341 ttcaccaaag gcgtgcgctc cgcgatgggc gacttcttcg ccaagatcta cggcagcctc
     2401 gaccagagcg cgcggcagac cgccgagacg cacgagatga tgcaggcgat gtatcagcgc
     2461 tttgccaaag agcacggcat cgagccgttt acccctcctc cgttctcgat gctgaagtac
     2521 cagaaggagg tcgagcggct ggagcgcgcg tacaacgtgc atttcaacac gctgtggaac
     2581 atggtcagca aggccaagtt cacgctgatg aaacgattct tcgagacgat cgcgatccgg
     2641 gtgaagcacg tgttcgacat cgcgaaccgc gacctggaaa gctggctgaa ggccgtgatg
     2701 gctccgctgg agacgcaggt gcgcgagcat cacctgcagc tgcgccgccg gctggaaagc
     2761 gtcaagcgca tccaccgggc gagcgatgag ctcgaggatc gcatcgacga gctcgagaac
     2821 gccgaggtcg ccgcgaaagc gcagatcggc gacctcgcgc gcgaggtggc cgcgatcgac
     2881 gcgatctgcg agcagtccga gccgctgccg ctcgccgcca acgcctagct gcttcgcgcg
     2941 cgaccgatca ggccggcacg ttgccggcct tggccgccat cagaccgcgg tacttcagca
     3001 gcagctgatc cttgctttcc acgaagtcgg ggtttaccgg tatgcaatcg accgggcaga
     3061 cttcgacgca ctgcggcgtg tcgaagtggc cgacgcattc ggtgcacttg cccggatcga
     3121 tcacgtagat ttccggaccc tgcgcgatcg cgccgttcgg gcactcgggc tcgcaaacgt
     3181 cgcagttgat gcattcgtcg gtaatgatca gcgccatcag gatctttcac gcgactgcgc
     3241 cgcacgctcc tcgtctgcct tggccttgag gcgggcgacg acggcaggcg gcacgaattt
     3301 gccgacgtcg ccaccgaagc gggcgatttc acgaacgatc gtcgatgaca tgaacaggta
     3361 ttgctcggcc ggggtgagaa acagcgtttc gacctcggga tggaggttgc ggttcatgcc
     3421 ggccatctga aactcgtact cgaagtccga caccgcgcgc aagccgcgca ggatcgtcgt
     3481 ggcaccgacc gagtgcacga aatccatcag caggccggaa aagcccgcga cctcgacatt
     3541 ggcgtacggc gccagcacct cgcgcgccat cgcaatgcgc tcgtcggcag tgaacatcgg
     3601 gcgcttgctt tcactgtccg caatcgcgac gatcacccgg tcgaacagct tggccgcgcg
     3661 ccgtacgatg tcctcgtgcc ctttggtgaa cgggtcgaag gttccaggat agacgatttt
     3721 cagcatgccc cgatctctcc gctcggccca agcgctccgc tcaagccgct gccggcaggc
     3781 ggcgcagcag atgataatgc acctgtccgg ccttgtcccc gcgcactatc gcgaaaccgg
     3841 caggcgcaac gacgggtgcg gaggactcga catagagcgc gccgtccgca gcgacccgcg
     3901 ctgaagctgc cggcagcagc gcctcccatt gcggagcggc aaacggcgga tcgaggaaga
     3961 cgacgtcgaa caagcgcgtt tcgcgcgcca ggaatgcttg cgcgtcggcg cagtgagcct
     4021 ccagcgtagc gcccagcatc tccgcggccg cgtgcagcgc gcgcaccagg tcgggcttgc
     4081 gatcgactgc gaccgcgagc gtggcgccgc gcgacagcgc ctcgaacgaa agcgccccgc
     4141 tgcccgcgaa cggatcgagc gtgctcgcgc cgtcgagatc ctggcccagc cagttgaaca
     4201 gcgtttcgcg aacccggtcg ggcgtcgggc gtatgtcggc gacggcggga aagcgaatgc
     4261 gccggccgcg ccaacggccg ccgatgatgc gaatctggtg ggcttgcggc aagcgtgcgc
     4321 catgcaccga aaggtagaat tgtacctttg cccgcagcgc ccaaatccga tgctcgacat
     4381 gttcaagcgc aagcccgatg gcggcgagcg cagctggtcc gaccgcctga agagcggcct
     4441 cggtctctcg cgcgagcgca tttcccgtcc gctgaccgcg ctgttcggtc gcgaggcgct
     4501 gtcggaggaa gcggtcgacg aattgcgcag cgccctcctg ggcgccgacg tcggcattcc
     4561 ggcgaccgag cacctgctcg acgagctcgt cgcccgctgg aagcgcgccg gcgccgatct
     4621 cgacacgcgc ggggtgctca agtcggcgct ggtcgagctg atcgcgccgc tcgagcagcc
     4681 actcgtcatc ggctcggcac ggcctttcgt catcatgctc gccggggtca acggtgcggg
     4741 caagacgacg tcgataggca agctggccaa gtacctgcag ggccagggac tctcggtgct
     4801 gctcgccgca ggcgatactt tccgcgccgc ggcgcgcgag cagctcgaaa cctggggtgc
     4861 gcgcaacaat atccaggtga tcgcgcagca tggaggtgat ccagcggccg tgatgttcga
     4921 tgcgatctcg gctgcgcggg cacggcgcat cgacgtcgtg cttgccgaca ccgccggccg
     4981 gctgccgacg cagatgccgc tgatggaaga gctcaaaaaa atccgccgcg tgatcgccaa
     5041 ggcgcaggaa ggcgcaccgc acgaaacgct gctcgtgctc gacgccaaca ccgggcagaa
     5101 cgcactggcg caggtcgccg cattcgacgc agcgctgggg ctgaccgggc ttgtcctgac
     5161 caagctcgac ggcacggcca aaggcggggt cgtcgccgct atcgccaaga cgcggccgct
     5221 gcccctgaag ctgatcggcg tcggcgaagg aatcgacgat ctccgccctt tccgcgccgc
     5281 cgagttcgcc gatgcgctca tcgggtaacg gaatggcgat ggccgtcgcg aagcggctgc
     5341 tgatcagtaa gcactaactc agtccggccc gagcgtcctg cgccaacggt atcgaccccg
     5401 gcgctcccgg ggcgcaggca acgcttgggg t
//
LOCUS       DATANS010000738         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_77958, whole genome shotgun sequence.
ACCESSION   DATANS010000738 DATANS010000000
VERSION     DATANS010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77958"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VHT22_10995"
     CDS             <1..371
                     /locus_tag="VHT22_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX3138129.1"
                     /translation="AVLRRAPATQPPGAPTTEQQVVEFGDFSFNLGTRSLARNGEQLP
                     LTTGEFALLKVLVQHPRTPLSRDKLMELARGREFGAFDRSIDVQISRLRKLIESDPAK
                     PAFIQTVWGFGYVFIPDGKT"
     gene            501..1832
                     /locus_tag="VHT22_11000"
     CDS             501..1832
                     /locus_tag="VHT22_11000"
                     /inference="COORDINATES: protein motif:HMM:NF027838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3138130.1"
                     /translation="MTLFPRSLLWRTVLLLAVLMVAGHFASLEIFRAAEREPRAVQIA
                     QQIASVVNLTRSALITADSAKRLDLLRDLSQQEGIQVYADAPNEPVEALPDRPITRLI
                     TLELQRRLGPDTQLLGTSDSMHGVWVSFKIDDLEYWVRLSRERIERAEQLRWIGWGAL
                     ILLLSVIGAFAIVARINRPLRELTRAATAIGRGDVPPPVVETGPAEVRTLSRAFNQMA
                     TDLQRADADRALLLAGVSHDLRTPLSRIRLGVEMLDDKTDAVLREGMVQDVEDIDAVI
                     NQFLDFARVTGEPGSGGELDLNELVHSVVDRYVRQGKQVSARTSPVPVLDLQPLAIQR
                     LVTNLIDNALRHGGDQVEVETSTTAGRARLSVLDRGPGIPAADAERMLQPFTRLNEAR
                     STPGSGLGLAIVDRIAKMHGGTVQLLPRSGGGLEARVEFGLPPAGGPDRAP"
     gene            complement(1829..>2255)
                     /locus_tag="VHT22_11005"
     CDS             complement(1829..>2255)
                     /locus_tag="VHT22_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015861083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX3138131.1"
                     /translation="TGDYLLLSEWGESEWRKLLAVTTSRPYKASEVVIQRGVADRALY
                     FVASGALEVGVTYVDGVSISSLAKIGAGSVIGEQSFFDSEPRSANVWAVTEGELLCLT
                     QERFHELAKAEPVLMRDLLFALGRILSLRLRNTSMRVRR"
BASE COUNT          389 a          781 c          713 g          372 t
ORIGIN      
        1 aggccgtgct gcggcgcgcg ccggccaccc agccgcccgg cgccccgacc accgagcagc
       61 aggtcgtgga attcggcgac ttcagcttca atctcggcac gcgcagcctc gcacgcaacg
      121 gcgagcagtt gccgctgacg accggcgaat tcgcgctgct gaaagtgctc gtgcagcatc
      181 cgcgcacgcc gctctcgcgc gacaagctga tggaactcgc gcgcggccgc gaattcggcg
      241 cgttcgaccg ctcgatcgac gtgcagattt cgcgcttgcg caagctcatc gaaagcgatc
      301 ccgccaagcc ggccttcatc cagacggtgt ggggtttcgg ctacgtgttc atacccgacg
      361 gcaagacgtg aggcgagagg cgtgaggcgt gaggagaaaa agccttggat cctgccagca
      421 tgtcgtttcg gtgcaagcaa cgatctcgca ctggtttgcg ggtttccgcc ttacccctca
      481 cgctttacgc cttacgctcc atgaccctct tcccgcgcag cctcttgtgg cgcacggtat
      541 tgctgctcgc ggtattgatg gtggccggtc atttcgcctc gctcgagatt tttcgcgcgg
      601 ccgagcgcga gccccgcgcg gtgcagatcg cgcagcagat cgcgagtgtt gtcaacctca
      661 cgcgctccgc gctgatcacg gcggattcag ccaagcgcct cgacctcctg cgcgacctct
      721 cgcagcagga aggaatccag gtttacgccg acgcgccgaa cgagccggtt gaagcactgc
      781 ccgaccggcc gatcacgcgg ctcattacgc tcgagctgca gcgccggctc ggcccggaca
      841 cccagctgct cggcaccagc gacagcatgc acggcgtgtg ggtgagcttc aagatcgacg
      901 acctcgaata ctgggtgcgg ctgtcgcgcg aacgcatcga gcgcgccgag cagctgcgct
      961 ggataggctg gggcgcattg atattgctcc tgtcggtcat cggtgcgttt gccatcgtcg
     1021 cgcgcatcaa ccgcccgttg cgcgagctga cgcgcgcggc caccgcgatc ggccgcggcg
     1081 acgtgccccc gccggtcgtc gaaaccggcc cggccgaagt gcgcacgctg tcgcgcgcat
     1141 tcaaccagat ggcgaccgac ctgcagcgcg ccgacgccga ccgcgcgctg ctgctcgccg
     1201 gcgtctcgca cgacttgcgc actccgctct cgcgcatccg acttggcgtc gaaatgctcg
     1261 acgacaagac cgatgctgtg ctgcgcgaag gcatggtgca ggacgtcgaa gacatcgacg
     1321 ctgtgatcaa ccagtttctc gatttcgcgc gcgtcaccgg cgagcccggc agcggcgggg
     1381 agctcgactt gaacgaactc gtgcattcgg tggtcgaccg ttacgtgcgc cagggcaaac
     1441 aggtatccgc gcgcaccagc ccggttcccg tactcgatct gcagccgctc gcgatccagc
     1501 gcctagtcac gaacctgatc gataacgcgc tgcgccatgg cggcgaccag gtcgaagtcg
     1561 aaacgtcgac gaccgccggc cgcgcgcgct tgtcggtgct cgaccgcggg ccgggcatac
     1621 ccgcggccga cgcggaacgc atgctgcaac cattcacgcg tctgaacgag gcgcgcagca
     1681 caccagggtc gggactgggg ctcgcgattg tcgaccgcat cgcgaaaatg cacggcggca
     1741 ctgtgcaact gctgccgcgc tcaggtggcg gactggaagc gcgcgtcgag ttcggtttgc
     1801 cgccggcagg cggccctgac cgggcgcctt agcgccgcac gcgcatcgat gtattgcgca
     1861 ggcgcagcga caaaatccgg ccgagcgcga acagcaggtc gcgcatgagc accggctccg
     1921 ccttcgcgag ctcgtgaaat cgctcctggg taaggcacaa gagctcgcct tcggtcaccg
     1981 cccagacgtt ggccgaacgc ggctcgctgt cgaaaaacga ttgctcgcca atcacgctgc
     2041 cggcgcctat cttcgcaagc gaacttatgc tcactccatc gacatacgtg acgccgacct
     2101 cgagcgcgcc ggacgcaacg aaatagagcg cgcggtccgc gaccccgcgt tgaatcacga
     2161 cttcgctcgc tttgtacggg cgcgaagtcg ttacggccag cagcttgcgc cactcgctct
     2221 cgccccactc gctgagcaaa aggtaatcgc cggta
//
LOCUS       DATANS010000739         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_332432, whole genome shotgun sequence.
ACCESSION   DATANS010000739 DATANS010000000
VERSION     DATANS010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332432"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /locus_tag="VHT22_11010"
     CDS             <1..1404
                     /locus_tag="VHT22_11010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX3138132.1"
                     /translation="PGLPVSSRERGWIIATVMLGTVSTITAATIINVAFPALIAEFHI
                     GHDSLQWVAAGFLGAMTTTMLATAWLVEAHGQRRTFIAALAIFLASSALGGASFSPEV
                     LIASRVLQGAAAGVLQPLAMIALFEVFPPERRGSAMGLFGLGIVLVPAIGPSIGGFLL
                     ENFGWRSIFLLPLPFCVAGLALGWRFLPGPHGSGPQKRFDWAGLVLLGATLASLLSVS
                     VVGHRAGWASIELIVIGVSTLLLLAGFIAWEFRASAPLLALRLFAGGGFRATALVALA
                     YGLGLFGTTYLIPVFVQDVAGYSPSRAGALLIIPGLGLAVTIALAGRWTDRIEPRRIV
                     IAGLALFALSSLLLAFAGRTTDFVILGLWLLIGRIGLGMIIPALSVAAVQALDESYIA
                     YAASAVNFVRQLGGAIGVNLLAVLLEWRLGAHGAAGAATAFQECFWVVTIAFAGATAA
                     AWSIRRHRPDNNEPVLR"
     gene            1414..1854
                     /locus_tag="VHT22_11015"
     CDS             1414..1854
                     /locus_tag="VHT22_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX3138133.1"
                     /translation="MYRKILVAYNGAPESRLALDEAVRIAPDASVEVHLAAVVHYPSA
                     YLLAGEYVPEVALADEREHMETDLKTAHGVLAAKGINVIDHLVVGEPVDVLTKLVAEL
                     GIDLLILGHPRSKSFALRWWRGSVDAILIERVRCSILVAADPKR"
     gene            complement(1861..>2524)
                     /locus_tag="VHT22_11020"
     CDS             complement(1861..>2524)
                     /locus_tag="VHT22_11020"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HEX3138134.1"
                     /translation="CAQAPDPPRFSTAPPGAVPGGWQTVLLSASKQPTEYTLVADEGV
                     VVLRASARGSASILAWRTDFDPRAFPMLSWRWKARPIAGADTRVRKSEDSPARVMVSF
                     SGNPASLKVSDRAAAAVAEAISGHPLPYAELMYIWGAKVPPESTTVSTLTSRIRMIAV
                     SSDEEGAGRWQSYRRNLAEDYRRVFGEEPGRVTAIELMTDTDNTGAVTETFYGDLSLS
                     AP"
BASE COUNT          345 a          842 c          848 g          489 t
ORIGIN      
        1 ccagggcttc cggtctcatc gcgcgagcgc ggctggatca tcgcgacggt gatgctcggc
       61 actgtctcga cgatcaccgc cgcgaccatt atcaatgtcg ccttccccgc gctgatcgcc
      121 gagttccaca tcggccacga ctcgctgcaa tgggtcgcgg ccggatttct cggcgcgatg
      181 acgacgacga tgctggcgac cgcatggctg gtcgaagcgc acgggcagcg ccgcacgttc
      241 atcgccgcgc tcgcgatctt tctcgcgtcg tccgcgctcg gcggggcgag cttcagcccc
      301 gaggtgctga tcgcctcgcg cgtgctgcag ggcgcggccg ccggcgtcct gcagccgctg
      361 gcgatgatcg ccctgttcga agtgtttccg ccggagcgcc gcggcagcgc gatgggactc
      421 ttcgggctcg gcatcgtgct ggtgcccgcg atagggccca gcatcggcgg ctttcttctg
      481 gaaaacttcg gctggcgctc gatctttttg ctgccgcttc ctttctgcgt cgcgggcctc
      541 gcgctgggct ggcgctttct gccgggcccg catggcagcg gaccgcagaa gcgcttcgac
      601 tgggccggcc tggtgttgct gggagccacc ctggcctcgc tgctcagcgt ttccgtcgtc
      661 gggcatcggg cgggatgggc gtcgatcgaa ctgatcgtca tcggcgtgtc gacgctgttg
      721 ctgctcgccg ggtttatcgc gtgggaattt cgcgccagcg cgccgctgct ggcgctgcgg
      781 ctcttcgccg gcggcggttt ccgcgccacc gcgctggtcg cattggctta cggtctggga
      841 ctcttcggta cgacttacct gattccggta ttcgtccagg atgtcgccgg ttattcgcct
      901 tcgcgcgcag gggccttgct tatcattccc gggctcggtc tggcggtgac gatcgctttg
      961 gcaggccgct ggaccgatcg catcgagccg cgacgcatcg tcatcgccgg gctcgcgttg
     1021 ttcgccctgt ccagcctgtt gctggcattc gccggccgga ccaccgactt tgtcatcctg
     1081 gggctctggc tgctgatcgg ccgcatcgga ttgggcatga tcattccggc gctttcggtg
     1141 gccgcggtgc aggcgctcga cgagagctac atcgcctacg ccgcctcggc ggtcaatttc
     1201 gtccgtcagc taggcggcgc gatcggcgtc aatctgttgg cagtgttgct cgaatggcgg
     1261 ctgggcgccc acggcgcggc cggtgcggca acggcctttc aagaatgctt ctgggtggtt
     1321 acgattgcgt ttgcgggcgc gactgcggcc gcctggtcga tccgcaggca tcgaccggac
     1381 aacaatgaac ccgtcctgcg ctaggaggcc gtcatgtacc gcaagattct cgtcgcctat
     1441 aacggcgctc cggagtcgcg tcttgcgctc gacgaagcgg tgcggatcgc gcccgacgcc
     1501 tcggtcgaag tgcatctcgc cgcggttgtc cattacccgt cggcgtacct gctggccggc
     1561 gagtacgtgc ccgaggtcgc gctcgccgac gagcgcgagc acatggagac ggacctcaag
     1621 acggcgcatg gggtgctcgc cgccaagggc ataaacgtca tcgatcacct ggtcgtcggc
     1681 gagccggtcg atgtcctgac caagctggta gccgaactgg gcatcgacct gcttatcctc
     1741 gggcatccgc gcagcaagtc gtttgcgctg cgctggtggc gcggctcggt cgacgcgatc
     1801 ctgatcgagc gcgtgcgctg cagcatcctg gtcgctgccg acccgaaacg ctgatcgcgc
     1861 ctaaggcgcc gaaaggctca agtcgccata gaaagtctcg gtgacggcgc cggtgttgtc
     1921 ggtgtcggtc atcagctcga tcgccgtcac gcggccgggc tcctcgccga agacccgacg
     1981 gtaatcctcc gcgagatttc gacgatagct ttgccagcgc cctgcgcctt cttcgtcgct
     2041 ggagaccgcg atcatgcgta tgcgcgaggt cagcgtgctt accgtggtcg attccggcgg
     2101 aactttcgcc ccccagatat acatcagctc cgcgtacggc agcggatgcc ccgagatcgc
     2161 ctcggccact gcggcggcgg cgcgatcact gactttgagc gacgccggat tgccggagaa
     2221 tgaaaccatc acgcgcgccg gcgagtcttc gcttttgcgc acgcgcgtgt cggcgccggc
     2281 gatcggccgc gctttccagc gccacgaaag catcggaaag gcccgcggat cgaagtcggt
     2341 ccgccaggca aggatcgacg ccgagcctcg ggcgctagca cgcaagacga ccacaccctc
     2401 gtcggcgacc agcgtgtact cggtcggctg cttgctcgcc gaaagcagaa cggtttgcca
     2461 gccgccgggc accgcaccgg gtggcgcggt cgaaaagcgc ggcggatcgg gtgcctgcgc
     2521 gcag
//
LOCUS       DATANS010000740         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_206844, whole genome shotgun sequence.
ACCESSION   DATANS010000740 DATANS010000000
VERSION     DATANS010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206844"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VHT22_11025"
     CDS             <1..653
                     /locus_tag="VHT22_11025"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3138135.1"
                     /translation="LTDDVADVALALVLMTGRGFVRLNRFVHAGKWEKGSPELTTKLS
                     GRKVGILGLGRIGKAIAERVAAMGMKVAYTGRKPQDVGYEYIADLKALAAAVDFLIVA
                     CPGGEATRNIVNADVLAALGKKGTLINIARGSIVDEPALVAALKAGTIKAAGLDVFAD
                     EPHIPAALTTMDNVVLLPHVGSATRETRQAMGDLCKANLDAWFADKKVLTLIPELK"
     gene            complement(684..1613)
                     /locus_tag="VHT22_11030"
     CDS             complement(684..1613)
                     /locus_tag="VHT22_11030"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX3138136.1"
                     /translation="MTSVRRAARRVPASGLFLVAVLTLVWGCNWPVLKLGVTELAPLT
                     FRGATLPFAALGLLLAARLSGDSIRIPREHWGKVLALALFNIAAWNGLILFGVQQMPA
                     GRSAILAYTMPVWTVLFGMALLHEPLSRRKIIGLVLGMLGMAVLLGEDVRHITRSPMG
                     TLFILAAAISWAFGTVLLRRWKPPVPQNTLTGWMMLIGWLPIAVCVPLFDEHPISSLA
                     HMSGTAWFAVLYNIFLAGTVAHWAWFTMARTLPVAVSSLSSLPVPVVGVLSGILFLGE
                     RPGPSEYIALALVLASLSAVLFAPATKPVVPPE"
     gene            complement(1636..>2248)
                     /locus_tag="VHT22_11035"
     CDS             complement(1636..>2248)
                     /locus_tag="VHT22_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138137.1"
                     /translation="IAALTGYAYGLRRRQLPHGTPTGSTVTGAVDPTGALSGPDAERI
                     GAEKARIIRSGEGEAAHLRQALADADAIRYQAAVFGEDRRRLFAALADVRGDNARYRQ
                     LVIDIENNAPPPILSGAAGPDDLKLIVGVGPVLERMLHNLGIMTFQQIAQWSERDIDE
                     FDARLPEFPGRIRRDQWETQARSLQQAKYGDGAAGLRPDGRRP"
BASE COUNT          416 a          740 c          762 g          330 t
ORIGIN      
        1 tgttgaccga cgacgttgcc gacgtcgcgc tcgcgctggt cctgatgacc ggccgcggct
       61 tcgtgcgcct gaatcgcttc gtgcacgcgg gcaagtggga aaagggaagc cccgagctga
      121 cgaccaagtt gagcggcaga aaggtcggca tcctgggcct gggcagaatc ggcaaggcga
      181 tcgccgagcg ggtggcggca atgggtatga aagtcgccta tacggggcgc aagccgcagg
      241 atgtcggtta tgaatacatc gccgacctga aggcgctggc cgctgccgtc gacttcctga
      301 tcgtcgcgtg tccgggcggt gaggcgaccc ggaatatcgt caacgccgat gtgctcgcgg
      361 cactcggcaa gaagggcacg ctgatcaaca tcgcgcgcgg gtcgatcgtc gacgagccgg
      421 cgctggtcgc ggcgctgaag gcaggcacga tcaaggcggc cggactcgac gtcttcgccg
      481 acgagcccca cattccggcg gcgctgacga cgatggacaa cgtcgtgctg ctgccgcacg
      541 tcgggagcgc aacgcgcgaa acgcggcagg cgatgggcga cctgtgcaag gcgaacctcg
      601 acgcctggtt cgccgacaaa aaagtgctga cgctgattcc cgagctgaag tagctcgcgc
      661 cggcggcgct ttgtgccgcg gcgttactcg ggagggacga ccggcttggt cgccggcgcg
      721 aacagcaccg cggagagcga tgccagcacc agcgcgagtg cgatgtactc gctcgggccc
      781 gggcgctcgc ccaggaaaag gattcccgac agcacgccca cgacaggcac cggcagcgac
      841 gaaagcgagc tgaccgcgac cggcagcgtg cgcgccatag tgaaccatgc ccagtgcgcg
      901 acggtgccgg cgagaaagat gttgtagagc actgcgaacc aggccgtgcc cgacatgtgg
      961 gccagcgaac ttatcggatg ctcatcgaac agcggcacgc agaccgcgat aggcagccag
     1021 ccgatcagca tcatccaccc ggtcaacgtg ttctgcggca ccggtggctt ccagcgccgc
     1081 agcagcacag tgccgaacgc ccacgatatc gcggcggcaa ggatgaacaa ggtccccatc
     1141 ggcgagcgcg tgatatgccg cacgtcttcg ccgagcagga ccgccatgcc cagcatgccg
     1201 agcacgagtc cgatgatctt gcgtctcgac agcggctcgt gcagcaacgc catgccgaac
     1261 agcacggtcc agaccggcat cgtgtatgcc aggatcgcgg accggccggc gggcatttgc
     1321 tggacgccga aaaggatcag cccgttccat gccgcgatgt tgaacagcgc gagcgccagg
     1381 accttgcccc agtgctcgcg cgggatccgg atcgaatcgc ccgacagccg cgccgccagc
     1441 aacaagccga gcgccgcgaa tgggagcgtc gcgccacgaa acgtcagcgg cgcgagctcg
     1501 gtgacgccga gcttcagcac cggccaattg catccccaga ccaaggtcaa gaccgcgacc
     1561 agaaaaagcc ccgaggccgg aacgcgccgc gcggcccgac gaaccgaagt catcgaatga
     1621 tcgcgcctgc ggtggtcatg gccgccgccc gtccgggcgc aggccggccg cgccgtcgcc
     1681 gtatttggcc tgttgcagcg agcgcgcctg cgtctcccat tgatcgcgcc ggattcggcc
     1741 gggaaactcg ggcaggcgcg cgtcgaattc gtcgatgtcg cgctcgctcc actgagcgat
     1801 ctgctggaag gtcatgatgc ccaggttgtg cagcatgcgc tcgagcaccg gtccgacgcc
     1861 gacgatcagc ttcagatcgt cgggtccggc cgcgcctgac aggatcggag gcggcgcgtt
     1921 gttttcgatg tcgatcacca actgtcgata gcgcgcgttg tcgccgcgca catcggcgag
     1981 cgcagcgaac agtcgcctcc ggtcttcgcc gaaaacggcg gcctgataac ggatcgcatc
     2041 tgcgtcggcc agcgcttgcc gcagatgggc tgcttcgcct tcgccgctgc gtatgatgcg
     2101 tgccttttct gcgccgatac gctcggcgtc tgggccggaa agagcgcccg ttggatcgac
     2161 tgcaccggtc accgtagagc cggtcggcgt cccgtgcggc agctgacgcc ggcgcaatcc
     2221 atacgcgtaa ccggtcagcg cggcgatg
//
LOCUS       DATANS010000741         2742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_458882, whole genome shotgun sequence.
ACCESSION   DATANS010000741 DATANS010000000
VERSION     DATANS010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458882"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1651
                     /locus_tag="VHT22_11040"
     CDS             <1..1651
                     /locus_tag="VHT22_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX3138138.1"
                     /translation="GGQILVANLLAQGADLAFCVPGESYLPVLDALYDARDQLRLIVC
                     RQEGGAAYMAEAYGKLTGRPGIAMVTRGPGASNAAVGIHTAAQDSSPMIVFVGQVGSD
                     FADREAFQEIDYRRMYGSSVKWVAQIDRADRVPEYVARAFRVAQSGRPGPVVLALPED
                     MLVASASVDDAARVGPTIVYPGSEAIAGLRARLEQARQPLVLVGGSGWSERACADLAR
                     FIAANDLPIACAFRNQDLIDNRDEHYAGEVGINPNPKLAARVRDADLLLVIGERLGEA
                     VTSGYTLLSVPNPAQALVHVHPGADELGKVYQPALAIAASLPAACAALADMAPIAAPA
                     WDGSAVEAHAEYLAWQSPRPMPGELDLWQVVGFLRERLPEDAILVNGAGNYATWLHRL
                     YRYPRYRTQLAPYSGSMGYGVPAAIAAKAVCPERIVVSWNGDGCFLMNGQELATAVQY
                     RLNVIFVVVDNGMYGTIRMHQEKHYPARVYGTDLVNPDFAALARAYGVHGESVNRTAE
                     FTPAFERALAHDGPSLLHLRIDPQAITMNATIAELRAQASR"
     gene            1668..>2742
                     /locus_tag="VHT22_11045"
     CDS             1668..>2742
                     /locus_tag="VHT22_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3138139.1"
                     /translation="MQAASDANTLSVTGLSVEFRTSERVVQAVRDLSFDIGRGETVAI
                     VGESGSGKSVTALSLMRLVEHGGGRITSGKLEFTRPSGDRIDLAQSNGATMRTIRGAE
                     IAMIFQEPMTSLNPVFAVGEQIAESIRLHQQMDFGAARREALRMLVQVRIPDAANVLS
                     RYPHQLSGGMRQRVMIAMALSCKPRLLIADEPTTALDVTIQAQILELIRALQEEMHMA
                     VLFITHDMGVVAEIADRVVVMVGGEKVEDGTAIRVFHAPEHPYTQALLAAVPTLGAMR
                     GTDAPARFVLRRQEPGAYTTPVQTPVHKAPPSARAPLLRVRGLKTQFPVRGGILGRVR
                     RRVHAVEQVSFDLYAGETLGLVGE"
BASE COUNT          433 a          916 c          930 g          463 t
ORIGIN      
        1 cggcggccag atcctggtcg cgaaccttct tgcccagggc gccgatctgg ccttttgcgt
       61 tcccggcgaa agctatcttc cggtgctcga cgccttgtac gatgcgcgcg accagctgcg
      121 gcttatcgtg tgccggcagg aaggcggcgc tgcgtacatg gccgaggcgt acggcaagct
      181 gaccggcagg cccgggattg cgatggtgac gcgcggaccg ggcgcatcga acgccgcggt
      241 cggcatccac accgcggcgc aagattcctc gccgatgatc gtcttcgtcg gccaggtcgg
      301 cagcgatttc gccgatcgcg aagccttcca ggaaatcgac tatcgccgga tgtacggcag
      361 cagcgtcaag tgggtcgcgc aaatcgatcg cgccgaccgt gttccggaat atgtcgcgcg
      421 cgctttcagg gtcgctcaat ccggacgccc gggtccggtg gtgctggcgc tgccggagga
      481 tatgctggtc gccagtgcgt ccgtcgacga cgccgctcgc gtcgggccga cgatcgtgta
      541 tccggggtcc gaagcaatcg cgggactgcg cgcacggctc gagcaggcgc gccagccgct
      601 ggtcctcgtc ggcggcagcg gctggagcga gcgcgcgtgc gccgacctcg cgcgttttat
      661 cgcggccaac gacttgccga tcgcgtgtgc attccgcaat caggacctca tcgataaccg
      721 cgacgaacac tacgcgggcg aagtcggcat aaacccgaac ccgaagcttg cggcgcgcgt
      781 gcgcgatgcg gacctgctgc tggtcatcgg cgagcgtctg ggtgaggcag tcacctcggg
      841 ctatacgctg ttgtcggtgc cgaatccggc gcaggcgctg gttcacgtgc accccggggc
      901 cgacgaattg ggcaaggtct atcagccggc gctggcgatc gctgcgtcgt tgcccgcggc
      961 ctgcgcggcc ctggccgaca tggcgccgat cgcggctccc gcctgggacg gcagcgccgt
     1021 cgaggcgcac gccgaatacc tcgcctggca gtcgcctcgg ccgatgcccg gcgaactcga
     1081 cctctggcaa gttgtcggat ttctgcgtga gcggctgccg gaagacgcga ttctcgtcaa
     1141 cggcgccggc aactacgcga cctggctgca tcgcctgtac cggtatccgc gttaccgtac
     1201 ccagctcgcg ccgtacagcg gctcgatggg ttacggcgta ccggctgcaa tcgcggccaa
     1261 ggccgtttgc cccgagcgca tcgtcgtatc atggaacggc gacggatgct ttctgatgaa
     1321 cgggcaggag ctcgcgaccg cggtccagta tcgtctgaac gtcattttcg tcgtcgtcga
     1381 caacggcatg tacggaacca tccgcatgca tcaggaaaaa cactatccgg cgcgtgtcta
     1441 cggcaccgac ctcgtcaacc cggatttcgc tgctctggcg cgcgcctacg gcgtgcacgg
     1501 cgagagcgtt aaccgcacgg cggaattcac gccggccttc gagcgcgcgc tggcgcatga
     1561 cggcccgtcg ctgctgcacc ttcgcatcga cccccaggcg atcacgatga atgcgacgat
     1621 cgccgaattg cgcgctcagg cgtcgcgatg agcgcgtgcc ggcgatgatg caagcggcgt
     1681 ccgacgcaaa cacgctttcg gttaccgggc tttcggtcga attccgcacg tccgagcggg
     1741 tcgtgcaggc ggttcgcgac ctttcgttcg acatcgggcg cggtgaaacg gtcgccatcg
     1801 tcggcgaatc gggctccggc aaatcggtga ccgcgctgtc gctgatgcgg ctggtcgagc
     1861 acggcggcgg ccgcatcaca tccggcaagc tggagttcac ccgccccagc ggcgatcgca
     1921 tcgacctcgc gcagtcgaat ggcgcgacga tgcgcacgat ccgcggcgcg gaaatcgcga
     1981 tgatcttcca ggagccgatg acgtcgctga acccggtctt tgccgtcggt gagcaaatcg
     2041 ccgaatcgat ccggctgcac cagcagatgg acttcggggc cgcgcgccgc gaggcactgc
     2101 gcatgctcgt gcaggtccgc atacccgatg ccgccaatgt tctgtcgcgc tatccgcatc
     2161 agctgtcggg cggcatgcga caacgcgtga tgatcgcgat ggcgctgtcg tgcaagcccc
     2221 gcctgctgat cgccgacgag ccgacgacgg cactcgacgt gacgatccag gcgcagattc
     2281 tcgagctgat ccgggccctt caggaagaga tgcacatggc ggtgctgttc atcacgcacg
     2341 acatgggcgt cgtcgccgag atcgccgacc gcgtcgtcgt catggtcggt ggggaaaagg
     2401 tcgaagacgg taccgcgatc cgggtcttcc acgctcccga gcacccctac acccaggcgc
     2461 tgctcgcggc agtgcccacc ttgggggcga tgcgtgggac cgacgcgccc gcgcgcttcg
     2521 tgctgcgccg gcaggaaccc ggtgcgtaca ccacgccggt acagactccg gtccacaagg
     2581 cgccgcccag cgctcgcgcg ccgctgctgc gggtgcgcgg gctcaagacg cagtttccgg
     2641 tgcggggcgg catcctgggc cgcgtgcggc ggcgcgtgca tgcggtcgaa caggtcagct
     2701 tcgacctcta tgcgggcgag acgctgggcc tggtcggcga at
//
LOCUS       DATANS010000742         2567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_622396, whole genome shotgun sequence.
ACCESSION   DATANS010000742 DATANS010000000
VERSION     DATANS010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622396"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VHT22_11050"
     CDS             complement(<1..191)
                     /locus_tag="VHT22_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3138140.1"
                     /translation="MRKRPVWLWIAAAAGYAFLYVPLVIVVLYSFNNSRLNAEWVGFT
                     LDWYYKLFHNEDMLAAAGNS"
     gene            complement(184..1110)
                     /locus_tag="VHT22_11055"
     CDS             complement(184..1110)
                     /locus_tag="VHT22_11055"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3138141.1"
                     /translation="MSKPALSMQARARLGRWLVSGPPVLYLLVFFAAPALIMVLASFR
                     TPGDFGGLAPLVEDGRLDLNLDSYARFFTESVYAQIFVKSVWYALLTTLFCLLLAYPL
                     AALIARSAKKHRDLLLLLVILPFWSNFLIRIYAWMIILGPNAALAKTVNGALGWLGHE
                     PVPLLFSSFAVLVCLVYVHLPFMVLPLYANLEKHDRALLDAAQDLGAGAWQRFWRITF
                     PLSLPGVYAGAALVFIPALGIFAIPDILGGPEDSLIGNVIKQQFLETRDWPFGSVLSI
                     VLTVVALALAGFAAWVGRRSLGNPSSAKGADA"
     gene            complement(1124..2242)
                     /locus_tag="VHT22_11060"
     CDS             complement(1124..2242)
                     /locus_tag="VHT22_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3138142.1"
                     /translation="MALLEIQNVTRRFGEFTAVDGVSIDIQAGEFFTLLGPSGCGKTT
                     LLRMIAGFDLPDGGRIVLNGMDLAERPPEERPVRTVFQSYALFPHMTVEGNIAFPLQM
                     AKTPGTAIPGKVQEALEDVRLTGFGKRYPHELSGGQKQRVAIGRALVTHPTVLLLDEP
                     LAALDAKLREEMQIELINLQKEVGITFVYVTHDQTEALALSHRIAVMNKGRVEQIDEP
                     SRLYSFPASRFVADFIGTCNLLEGAIASSSDGIVAVEVAGLGTVRVAAALPAAPPAGT
                     IALRPEKIRVQAAAAASAAADRPDNHFLGTVTEYLYLGDVTVYIVTTPAGRKLETLLA
                     NSGAGRTKFFEVGDAVEVSWPVDAGHFIGQPDRSVSST"
BASE COUNT          481 a          816 c          854 g          416 t
ORIGIN      
        1 gaattgcccg ccgcggcgag catgtcctcg ttgtggaaca gcttgtaata ccagtcgagc
       61 gtgaagccca cccattcggc gttgagcctc gaattgttga acgaatacag cacgacgatg
      121 acgagcggca cgtagagaaa cgcatagccc gcggccgccg cgatccacag ccagaccggg
      181 cgcttacgca tcggctcctt tcgcgctcga cgggttgccg aggctgcgcc gtcccaccca
      241 cgcggcgaag cccgcgagcg ccagcgcgac cacggtcagc acgatcgaca gaacgctccc
      301 gaacggccag tcgcgcgtct ccaggaactg ctgcttgatg acgttgccga tcaggctgtc
      361 ttcggggccg ccgaggatat ccggaatggc gaagatgccc agcgcgggaa tgaacaccag
      421 cgctgcgccg gcatagacgc cgggcagcga cagcgggaac gtgatgcgcc agaaccgctg
      481 ccatgcgccg gcgcccagat cctgcgcggc atcgagcaaa gcccggtcgt gcttttccag
      541 gttggcgtag agcggcaaca ccatgaatgg caggtgcacg tagaccaggc agacgagcac
      601 cgcgaacgag ctgaaaagca agggcacggg ctcgtgcccg agccagccga gcgcgccgtt
      661 gacggtcttg gccagcgcgg cgttgggacc gaggatgatc atccaggcgt agatgcggat
      721 caggaaattg ctccagaagg gcaggatcac cagcagcagc aggaggtcgc gatgtttttt
      781 cgcgctgcgc gcgatcagtg cggccagtgg atacgcgagc agcaggcaga acagcgtcgt
      841 cagcagcgca taccataccg atttgacgaa gatctgcgcg tacaccgatt cggtgaaaaa
      901 gcgcgcgtag ctgtcgagat tgagatcgag cctgccgtcc tcgacgagcg gcgcgagccc
      961 gccgaaatcg cccggcgttc gaaaggaggc caggaccatg atcagcgccg gcgccgcaaa
     1021 aaagaccagc aggtagagca ccggaggtcc gctgaccagc catcggccga gccgggctcg
     1081 cgcctgcatc gaaagagccg gtttgctcat cgaatagggt tcgtcaagtc gagctcactg
     1141 accgatcggg ttggccaatg aagtggccgg catcgaccgg ccagctcact tcgaccgcat
     1201 ccccgacttc gaaaaatttc gtgcgtcccg cgcccgaatt ggcgagcagc gtctcgagct
     1261 tgcggcccgc gggcgtggtg acgatgtata cggtcacgtc gcccaggtag agatattcgg
     1321 taacggtgcc gaggaagtga ttgtccgggc ggtcggccgc tgcggatgcc gctgcggccg
     1381 cctgcacgcg gatcttttcc ggacgcaggg cgatcgtgcc tgcgggcggc gccgcgggca
     1441 gtgcggccgc tacgcggacg gtgccaagac cggcaacctc gaccgcgacg atgccgtccg
     1501 agctcgacgc gatcgccccc tcgagcaggt tgcaggtgcc gatgaagtcg gcaacgaagc
     1561 gcgacgccgg aaagctgtaa aggcgcgagg gttcgtcgat ctgctcgacc cgtcccttgt
     1621 tcatcaccgc gatccggtgc gacagggcga gcgcctcggt ctgatcgtgc gtcacgtaga
     1681 cgaaagtgat gccgacctcc ttctgcagat tgatcagctc gatctgcatc tcctcgcgca
     1741 acttcgcgtc gagcgcagca agcggctcgt cgagcagcag cacagtcgga tgggtgacca
     1801 gcgcgcgccc gatggccacg cgctgcttct ggccgccgga aagctcgtgt ggatagcgct
     1861 tgccgaagcc ggtcaggcgg acatcttcga gcgcctcctg caccttgccg ggaatcgccg
     1921 tcccaggcgt cttcgccatt tgcagtggaa aggcgatgtt gccctccacc gtcatatgcg
     1981 gaaacagcgc atagctctgg aagaccgtgc gcaccggccg ttcttcgggc ggccgctcgg
     2041 cgaggtccat gccgttgagc acgatgcgcc cgccgtcggg caggtcgaat cctgcaatca
     2101 tgcgcagcaa ggtcgtcttg ccgcagccgg agggcccgag cagtgtgaaa aactcgccgg
     2161 cttggatgtc gatgctgacg ccgtcgaccg cggtgaactc gccgaatcgg cgcgtcacgt
     2221 tctggatttc gagcagcgcc atcgcggacg gactcggccc gttcagaaga tggcggattg
     2281 tagcaaaagg aggtcctttc gccgggaatc gccgccgcgg ccgccagtca tcgatgtcaa
     2341 ccgatcggcg gcggcggacg ttgatcggcg acggtgaatg ctgatgtgga tgcctgccat
     2401 gcgaggtctt gctctgctcg ctgcggccgc ggcgctacct ctgccgcttt tcgccgccga
     2461 cagctatcag ctcgatccgt tcgagcaggc gactgcggga ttcgccgcct gcccgacggt
     2521 agcgccgccg gtgctgactt ccgagcagat gcgcgcgcag gcgcact
//
LOCUS       DATANS010000743         3328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_541276, whole genome shotgun sequence.
ACCESSION   DATANS010000743 DATANS010000000
VERSION     DATANS010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541276"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1510
                     /locus_tag="VHT22_11065"
     CDS             <1..1510
                     /locus_tag="VHT22_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3138143.1"
                     /translation="GSSMVSVPAAGRNDPCPCGSGKRYKNCHGALAASAPPSGAVSAP
                     PPASALDLSVQQAIALHQQGDTAAAERIYREALTVQPGHPLAQHFLGVILYQRRDVAA
                     ALPLLQRSVEAVPAEPEFHNNLGLALAAGEREEEAIAAFRAALALKSGHAVAWNNLGL
                     ALQSVNDVDGAISAFRRAIALQPEFEHAHWNLALALLLDGQYAEGWREYESRFALSEL
                     GKDRQVFAGPAWDGSAPTGKTLLLYTEQGLGDALQFARFVAPLARTGAHCIVYCPPAL
                     APLLATAPGVAQAVADADRLPSYDAHLPLLSLPRLLGTTVDAIPADVPYITVAPERRA
                     AARKLIAASPHRLNVGIVWTGNPANPGNRIRSAGLDALDPLFEVEGIDWYSLQQGRTT
                     ADFTPAARKARMRPLPADWALVDTAALITELDLVISVCTSIAHLSGALARPTWTMLAC
                     AADWRWLQGRADSPWYPTMRLFRQPHPRDWNAVARSIASELRALVSALTATE"
     gene            1542..>3328
                     /locus_tag="VHT22_11070"
     CDS             1542..>3328
                     /locus_tag="VHT22_11070"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3138144.1"
                     /translation="MSQTAASAALREARAAWQRGDASAAESHSRRALELDAGDGRAWA
                     MLGVALRQRDPAEAEAALRRAAVIGPRDPDAHFHLGNLLREQGRPAAAIESYERALAL
                     APDHPSLLNNYALALDAVGAGGRAQATYRRILESRPDHRQALRNLAHSLCSARRFNEA
                     AAHCAHYLKLHPEGDARVWIDHGICGHARGDDVAAEASFRRALALAPGDAVAQTNLAS
                     VLIDRGDFAAAEAPLSGAVADDPQFLYAAALLAYCRQHLCWWDGLAALHARVVRGIEQ
                     DESALVNAFAALSVPMSAANQLRAARRWAQSLAPPEPVAARAPHPRGSALRLGYVSSD
                     FRTHAVAFLATEVWERHDRARFETHAYSIAPPEDSPLGHRVAAAFSRFIDCTADAPDD
                     TAQRIRDDGIDILIDLNGYTTHARSEIFALRPAPVQINWLGYVGTLGAAWYDYVITDR
                     FATPAAQQAFFTERFLTMLHCCAPSDTRRVAAETPSREACGLPAQGFVFACFNAPYKI
                     LPPVFDVWMRLLREVPGSTLWLAQQGPAREHLRQEAAARGIDPERLTFAARLPPDRHL
                     ARHAHADVCLDTLPYNAGTTANDALFMGVP"
BASE COUNT          488 a         1188 c         1110 g          542 t
ORIGIN      
        1 gggtagcagc atggtctcgg tcccggccgc gggaagaaac gatccatgtc cgtgcggcag
       61 cggcaagcgc tacaagaact gccacggcgc gctggctgcg agcgcgccgc cgtcgggcgc
      121 cgtgtcagcg ccgccgcctg cttcggcgct cgatctctcc gtccagcagg cgattgcgct
      181 gcaccagcag ggtgataccg cggccgccga gcggatctac cgggaagcgc tcacggttca
      241 gcccggccat ccgctggcgc agcatttcct gggcgtaatc ctctatcaac gccgggacgt
      301 ggcggcagcg ctgccgctgc tgcagcgctc ggtcgaggcg gttcccgccg agcccgagtt
      361 ccataacaat ctggggctgg cgctcgcggc gggtgagcga gaggaagaag cgatcgccgc
      421 cttccgcgcg gcactcgcgc tcaagtccgg ccacgcggtc gcctggaaca acctcgggct
      481 cgcgctgcaa tcggtcaacg acgtcgacgg cgcaatcagc gcgttccgcc gcgcgatcgc
      541 gctgcagccc gaattcgagc acgcgcactg gaatctcgcg cttgcgctgt tgctcgacgg
      601 acagtatgcc gagggctggc gcgagtacga gtcgcggttc gcgctgtccg agctcgggaa
      661 ggaccggcag gtcttcgcgg gccccgcgtg ggacggctcg gcgccgacag gtaagacctt
      721 gctgctctac accgagcaag gtctgggcga tgccttgcaa ttcgcgcgct tcgtcgcacc
      781 gctggcgagg acaggcgcgc actgcatagt ctactgtccg ccagcgctgg cgcctttgct
      841 cgccacggct cctggcgtcg cacaggcggt cgccgacgcg gatcgactgc cgagctacga
      901 cgcgcatctc ccgctgctgt cgctgcccag gttgctcggc acgacggtcg atgccatacc
      961 ggcggacgtg ccttatatca ccgtcgcgcc ggagcggcgt gccgcagcgc gcaagctgat
     1021 cgcggcgtcg ccgcatcgac tcaacgtcgg catcgtctgg accggtaatc cggccaatcc
     1081 gggcaatcgc atccgttcgg cgggtctgga tgcgctcgat ccgctgttcg aagtcgaagg
     1141 catcgactgg tattcgctgc aacaagggcg cacgactgcc gatttcaccc cggccgcgcg
     1201 caaagctcgc atgcggcccc tgcccgccga ctgggcgctg gtcgacaccg cggcgttgat
     1261 caccgagctc gatctggtga tcagcgtgtg taccagcatc gcccatctgt cgggggcact
     1321 agcccgaccc acatggacga tgctcgcctg cgccgccgac tggcgctggc tgcagggccg
     1381 cgccgacagt ccgtggtatc cgacgatgcg cctgttccgg cagccgcatc cgcgcgactg
     1441 gaacgccgtg gcgcgatcga tcgcctccga gctgcgcgcg ctggtctcgg cgctgacggc
     1501 gaccgagtga tgaagtcccg accgatcgcg cggacgatcc cgtgagccag accgctgcca
     1561 gcgccgcgtt gcgcgaggcg cgggccgcct ggcaacgcgg cgacgcaagc gccgccgaat
     1621 cgcacagccg tcgcgccctc gagctcgatg ccggcgatgg gcgcgcctgg gcaatgcttg
     1681 gcgtcgcgct tcgccagcgc gatccggcgg aggccgaggc ggcgctgcga cgcgcggcgg
     1741 tcatcggtcc gcgcgatccc gatgcgcact tccatctcgg caacctgttg cgcgaacagg
     1801 gccgtcccgc ggcggcgatc gaatcgtacg aacgggcgct cgctctcgcc cccgaccatc
     1861 cgagcttgct caacaattac gctctcgcgc tcgacgcggt aggcgcaggc ggacgcgcac
     1921 aagcgacgta ccgccgcata ctcgaatcac gacccgatca ccgccaggcg ctgcgcaatc
     1981 tggcgcattc gctttgctcc gcgcgccgct tcaacgaggc cgccgcgcat tgtgcgcact
     2041 atctgaagct gcacccggaa ggcgatgcgc gcgtgtggat agatcatggc atctgcgggc
     2101 acgcccgcgg cgacgacgtt gcggccgaag cgagcttccg ccgcgccctg gcactcgcgc
     2161 cgggagatgc ggtggcgcag accaacctgg cgtcggtatt gatcgaccgc ggcgatttcg
     2221 ccgctgccga ggcgccgctt tccggcgccg tcgccgacga tccgcagttc ctgtacgcgg
     2281 ctgctttgct tgcctattgc cgtcagcatc tatgctggtg ggacgggctc gccgcgttgc
     2341 acgcgcgcgt cgtgcgcggc atcgagcagg acgagagcgc gctggtcaat gctttcgccg
     2401 cgctgtcggt cccgatgtcg gcggccaatc agctgcgcgc cgcgcgccgc tgggcgcaaa
     2461 gcctcgctcc gcccgagcct gtcgccgcgc gcgcgccgca cccgcgcggt tcggcgttgc
     2521 ggctgggtta cgtctcgtcc gactttcgca cccatgccgt tgcgttcctg gcgaccgaag
     2581 tatgggagcg acacgatcgc gcacgatttg agactcacgc gtattcgatt gcgcctccgg
     2641 aagactcgcc gctcggtcac cgcgtcgccg ccgcgttctc ccgattcatc gattgcaccg
     2701 ccgacgcccc cgacgatacc gcgcagcgaa tccgcgatga cggcatcgac atcctgatcg
     2761 atctgaatgg ctatacgacg catgcgcgaa gcgaaatctt tgcgctacgg ccggcgcccg
     2821 tgcagatcaa ctggctgggc tacgtcggaa cgctgggcgc ggcgtggtac gactatgtga
     2881 tcaccgatcg cttcgcgaca cccgccgccc agcaggcctt tttcaccgag cgcttcctca
     2941 ccatgctgca ttgttgcgca cccagcgaca cccgccgggt ggccgctgaa actccgtcgc
     3001 gcgaggcctg cggcttgccg gcgcaaggat tcgtgttcgc ctgcttcaac gcgccgtaca
     3061 agatcctgcc gccggtgttc gatgtctgga tgcggctgct gcgcgaggtt ccgggcagca
     3121 cgctgtggct cgcgcagcaa ggcccggcgc gcgagcatct gcggcaggaa gccgccgctc
     3181 gcggcatcga cccggagcgg ctgacattcg ccgcccggtt gccgcccgat cggcatctcg
     3241 cgcgccatgc gcacgccgac gtctgcctcg acacgctgcc gtacaacgcg ggcacgacgg
     3301 cgaacgatgc gctgttcatg ggggtacc
//
LOCUS       DATANS010000744         4006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_245528, whole genome shotgun sequence.
ACCESSION   DATANS010000744 DATANS010000000
VERSION     DATANS010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245528"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1833
                     /locus_tag="VHT22_11075"
     CDS             <1..1833
                     /locus_tag="VHT22_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX3138145.1"
                     /translation="SEKPVIAAIGGSCMGGGLELALGCHFRVAVPHAQIALPEVRLGL
                     LPGAGGTQRLPRAIGVEAALNMIVSGASMPCERFRGTALFDAIVDGDLLQGALAFASS
                     ERAARRPLPRVRDIVIDYPNADALFQFARNTVGAMARGFPAPLKCIDAVAAAVTTPFD
                     DGLRYERELFIQLAQTPESRALRHAFFAERAAAKIPDVPSGTAPRAINAAAVIGAGTM
                     GGGIAICLLDAGIPVTLLETKQEALDKGITTIRQHYDKALAKGRLDAVELQRRIGMLK
                     PTLAYDDLAGADLVIEAVFEDLAVKKVVFERLDAVAKPAAILASNTSTLDLNKIAGFT
                     ARPQNVVGMHFFSPANVMKLLEVVRGARTASDVLVTVMTVAKRMRKTAVVSGVCDGFI
                     GNRMIEQYLRQALFLLEEGASPGQVDRALEKFGMAMGPFRMSDLAGNDVGWYIRKRRY
                     VEKPDVVYSRIADRLCELGRFGQKTGLGWYRYEPGRRDALPDPAVDALIASYRSEHGM
                     APRKIADREIVDRCIYALVNEGARILEDGIASRASDIDVVYLTGYGFPLYRGGPMQYA
                     DTIGLFNVIRAMHRFAATGRGDPAFWQPAPLLARLAAEGKTFNS"
     gene            1845..3023
                     /locus_tag="VHT22_11080"
     CDS             1845..3023
                     /locus_tag="VHT22_11080"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX3138146.1"
                     /translation="MTDAVVVSYARTGLAKSWRGALNMTHGATVGAHAVRHAIERAGL
                     APGEVDDVLIGSAFPEGATGHNIARQIAIRAGCPVSVPGATVNRFCSSGLQTIALAAQ
                     RIIAGEAETFVAGGVESISCVQNEMNKHMLRESWLVENKPAIYWPMLETAENVARRYG
                     ISREAQDQYGARSQQRAAAAAAAHRFDAEIVPLTVTMGVADKESNRLYTREVTLASDE
                     GIRADTTYDAVARIKPALPGGVIAAGNASQFSDGAAACVVMSDKLAAKRGLEPLGIFR
                     GFAVAGCEPDEMGIGPVFAVPKLLARTGVKIGDIGLWELNEAFAVQVIYCRDKLGIPD
                     DRLNVDGGAIAVGHPYGVSGTRLTGHALLEGKRRGVKYVVVTMCIGGGMGAAGLFEIA
                     "
     gene            3086..>4006
                     /locus_tag="VHT22_11085"
     CDS             3086..>4006
                     /locus_tag="VHT22_11085"
                     /inference="COORDINATES: protein motif:HMM:NF022821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3182 family protein"
                     /protein_id="HEX3138147.1"
                     /translation="MTPATKRGRIVVTYSCRASRHHESVTLRELASRLAAIKGCGFVG
                     EYDPALRYSDPLYFVPNDTLVGIDAARRLGIKGEADLFGGVVPFAFTATKTITHPLPD
                     AESHSPEGWSPEFADRVRDLVVSGYSAFARRDARVAGMRLLELGRVRLKLPTGIGGLG
                     QSIVEDERALDGELGSLDDAALSRDGLVLEQDLADVVTHSVGQVCVDDMRASYCGVQR
                     LTTNHHGEQVYGGSDLTIARGGFDRLIKLALASEVETAIHQARGYHTAAMASFRGMFA
                     SRCNYDVVQGIDHRGQWRSGVLEQSWRAGGA"
BASE COUNT          683 a         1282 c         1340 g          701 t
ORIGIN      
        1 agcgaaaagc cggtcatcgc cgcgatcggc ggcagctgca tgggcggcgg actggagctg
       61 gcactcggct gccacttccg cgtcgccgtg cctcacgcgc aaatcgcact gccggaggtc
      121 aggctgggtc ttctgccggg cgccggaggc acgcaaaggc tgccacgcgc gatcggtgtc
      181 gaagccgcgt tgaacatgat cgtttccggc gccagcatgc cttgcgaacg ctttcgcggc
      241 acggcattgt tcgacgccat cgtcgacggc gatctgttgc agggcgcgct ggcgttcgct
      301 tcgagcgaac gcgccgcacg gcgcccgttg ccgcgcgtgc gcgatatcgt tatcgactat
      361 ccgaatgccg acgctctgtt ccagtttgca cgcaacaccg tcggcgccat ggcccgcggc
      421 tttcccgccc ccctcaaatg catcgacgcg gtggcggcgg ccgtcacgac gcccttcgat
      481 gacggcttgc gttacgagcg ggagctgttc attcagcttg cgcagacacc ggaatcgcgc
      541 gcgctgcgcc acgcattttt cgccgagcgc gcggcggcga agatccccga tgttccttcg
      601 ggcaccgcgc cgcgcgcgat caacgccgcg gcggtgatcg gggccggaac gatgggcggt
      661 ggcattgcga tctgcctgct cgacgccggc atcccggtca cgttgctcga aacgaaacag
      721 gaagcactcg acaagggcat aacgactatc cggcagcact acgacaaggc tctcgccaag
      781 ggccgcctcg atgccgtcga gctccagcga aggatcggca tgctgaagcc gacactggcg
      841 tacgatgatc ttgccggcgc cgatctcgtc atcgaggcgg tgttcgagga cctggcggtg
      901 aagaaggtcg tgttcgagcg tctggacgcg gtggcgaagc ccgctgcgat cctcgccagc
      961 aatacctcta ccctggatct gaacaagatc gccggcttca ccgcgcgtcc gcagaatgtc
     1021 gtcggcatgc attttttcag tcctgccaac gtaatgaagc tgctcgaggt cgtgcgcggc
     1081 gccaggaccg cgagcgacgt gctggtcacc gtcatgactg tcgccaagag gatgcgcaag
     1141 accgccgtcg tctcgggcgt atgcgacggc ttcatcggga accgcatgat cgagcagtat
     1201 ctgcgccagg cgcttttttt gcttgaggaa ggcgcctcgc ccggacaagt cgatcgtgcg
     1261 ctggagaaat tcggcatggc gatggggccg tttcgcatga gcgacctcgc cggcaacgac
     1321 gtcggctggt acattcgcaa gcgccgctat gtcgagaagc cggatgtggt ctattcgcgt
     1381 atcgccgacc ggctttgcga gcttggccgc ttcggccaga agaccggtct gggctggtac
     1441 cggtacgagc cgggacgccg cgatgcgctt cccgatcccg cggtcgacgc cttgatcgcg
     1501 agctaccgga gcgagcatgg catggcgcct cgaaagatcg cagaccggga gatcgtcgac
     1561 cgctgcatct atgcgctggt caacgagggt gcgcgcatat tggaagatgg catagcatcg
     1621 cgcgcctcgg acatcgacgt cgtctatctg acaggctacg gctttccgct ctatcgcggc
     1681 gggccgatgc aatacgctga caccatcggc ctgttcaacg tgatccgcgc gatgcaccgg
     1741 tttgccgcca cgggccgcgg cgatccggca ttctggcagc cggcgccgct gctcgcccgg
     1801 ctcgccgccg aaggcaagac gttcaacagc tgaggcatgc cgtcatgacc gacgcagtcg
     1861 tcgtatccta tgcgcgcacc ggactcgcga aatcctggcg cggcgcgctc aacatgacgc
     1921 atggcgcgac cgtgggtgcc cacgccgtac ggcacgcgat cgagcgcgcg ggactggcgc
     1981 cgggagaagt cgacgacgtc ctgatcggat ccgcgtttcc cgaaggcgcc accggacaca
     2041 acatcgcgcg ccagatcgcg atccgtgccg gttgcccggt cagcgtcccc ggcgcaacgg
     2101 tcaatcgttt ctgctcatcg ggcctgcaga cgatcgcgct ggccgcgcag cgcatcatcg
     2161 caggcgaagc cgagacgttc gtcgcgggtg gcgtcgaatc gatctcgtgc gttcaaaacg
     2221 aaatgaacaa gcacatgttg cgcgaatcgt ggctcgtcga gaacaagccc gccatctact
     2281 ggccgatgct ggagaccgca gagaacgtcg cgcggcgcta cggcatttcc cgggaggcgc
     2341 aggaccagta cggcgcgcgc agccagcagc gcgcggccgc agccgcggca gcgcaccggt
     2401 tcgacgccga aatcgtcccg ctgacggtga cgatgggggt tgccgacaaa gagtccaatc
     2461 gtctgtacac gcgcgaagtc acgctggcgt cggacgaagg cattcgcgcc gatacgacct
     2521 acgacgccgt ggcgcggatc aagcccgcgt tgcccggcgg cgtcatcgcc gccggcaacg
     2581 ccagccagtt ctccgacggc gcagcggcat gcgtcgtcat gagcgacaag cttgccgcga
     2641 agcgtggcct cgagccgctg gggatattcc gcggcttcgc cgtcgccggc tgcgaacccg
     2701 acgaaatggg catcggaccg gtttttgccg taccgaagct gctcgcgcga accggcgtaa
     2761 agatcggcga catcggtctg tgggagctca atgaggcgtt cgccgtgcag gtcatctact
     2821 gccgcgacaa gctgggaata cccgacgatc gcctgaacgt cgacgggggc gcgatcgccg
     2881 tcggacatcc ttacggcgtg tcggggactc ggctgaccgg gcacgcattg ctcgagggca
     2941 agcgtcgcgg ggtcaaatac gtcgtggtga cgatgtgcat aggcggcggc atgggcgccg
     3001 ccggcctgtt cgagatcgcg taagcattcg ctgagacctt catgtcggca gcgtcggtct
     3061 tgcgggcggc ggaagatcct gaccgatgac gccggcaacg aagcgcggcc gcatcgtcgt
     3121 cacctattcg tgccgtgcct ctcgccatca tgaaagcgtg acgctgcggg agctcgcgtc
     3181 ccggctggcc gcgatcaagg gctgcggctt cgtgggtgaa tacgatcctg cgcttcgcta
     3241 ttcggaccct ttgtacttcg tgccgaacga tacgctcgtc gggatagacg ccgcacggcg
     3301 cttgggcata aaaggggagg cggatttgtt cggcggtgtc gtgcccttcg cattcaccgc
     3361 cacgaagacg atcacccatc cattgcccga tgccgagtcg cactcaccgg agggatggtc
     3421 tccggaattc gcggatcgcg ttcgcgacct ggtggtttcg ggttactcgg cgtttgcgcg
     3481 gcgcgatgct cgcgtcgccg ggatgcgact gctcgaactg ggacgcgttc gcctgaagct
     3541 tcctacgggg ataggtggcc ttgggcagtc gatcgtcgaa gatgaacggg cgctcgatgg
     3601 agagctcggg tccctcgatg acgccgcact gtcgcgcgac ggccttgtgc tcgaacagga
     3661 cctggcggac gtggttacgc acagcgtcgg acaggtctgc gtggacgata tgcgcgcaag
     3721 ctactgcggc gtgcagcggc tcacgaccaa tcatcacggc gagcaggtgt acggaggttc
     3781 cgacctgacc attgcacgcg gcggcttcga ccggttgatc aagctcgcgc tggccagcga
     3841 agtcgagacc gccatccacc aggcccgcgg ctatcacaca gccgcgatgg cgtcgtttcg
     3901 cggcatgttc gcatcgcgct gcaactatga tgtcgtccag ggtatcgatc accggggaca
     3961 gtggcgctcg ggcgtgcttg agcaatcctg gcgcgctggc ggcgcg
//
LOCUS       DATANS010000745         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_622414, whole genome shotgun sequence.
ACCESSION   DATANS010000745 DATANS010000000
VERSION     DATANS010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622414"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VHT22_11090"
     CDS             complement(<1..200)
                     /locus_tag="VHT22_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3138148.1"
                     /translation="MAAVSIRSVQKRFGSTPVIRGVDVEIADGEFCVLVGPSGCGKST
                     LLRMLAGLEEITGGEIWIGSRVV"
     gene            complement(243..1214)
                     /locus_tag="VHT22_11095"
     CDS             complement(243..1214)
                     /locus_tag="VHT22_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEX3138149.1"
                     /translation="MARALITDQNYEDLALERELFGNAGIELAVAQCSTEDDVLAAGR
                     NFDAFLVQYAPIGARALAQLPRLGIISRIGAGYDTIDTDACAKYGVWLANSPDYGVGE
                     VATHALALGLALIRNVVAYNRDIAGGRWHYLSSGRLRRASELTLGIVGLGRIGKRMAH
                     VSRNVFRRVVACDPYLIDGDFPAYVERATLADLFEQSDLVSLHVPLNDETRSIVDATM
                     LSRMRPGSYLVNTARGALVDVDDLLAAVDSGILAGAALDVLPVEPVPADSRLLGHPKV
                     ILTPHAAFYSVQAEIELRRKAAQNIVTWLKTGRPEYVVVAGTRRYAP"
     gene            1372..>1504
                     /locus_tag="VHT22_11100"
     CDS             1372..>1504
                     /locus_tag="VHT22_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025816.1"
                     /note="catalyzes the formation of
                     tetrahydropteroyl-L-glutamate and methionine from
                     L-homocysteine and
                     5-methyltetrahydropteroyltri-L-glutamate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX3138150.1"
                     /translation="MLLPTTIAGSLPKPAWLAPPKQLWAPWLLEGSLLEEGKRDAVRL
                     "
BASE COUNT          267 a          492 c          492 g          253 t
ORIGIN      
        1 acgacccggg aaccgatcca gatttcaccg ccggtaattt cctcgaggcc ggcgagcatg
       61 cgcagcagcg tcgacttgcc gcagccggac gggccgacca gcacgcagaa ctcgccgtcg
      121 gcaatctcga cgtcgacgcc gcgaatcacc ggcgtggatc cgaagcgctt ttgcactgaa
      181 cgtatgctga ctgctgccaa tgctctcctt tccgtcgcgc ctgatgcgct cggatgatcc
      241 gcctacgggg cgtagcgccg ggtccctgca acgacgacgt attcgggacg accggtcttg
      301 agccacgtga cgatattctg tgctgccttg cgccgcaatt cgatctccgc ctgcacgctg
      361 tagaacgcgg catgcggcgt caggatcacc ttcgggtggc cgagcagccg gctatcggcg
      421 ggaacgggtt cgaccggcag gacatccaga gcagcgccgg caagtattcc actatcgaca
      481 gcggcaagca aatcgtcgac gtcgaccagc gcgccgcgcg cggtattgac caggtaggat
      541 cccggccgca tgcgcgacag catcgtcgcg tcgacgatgc tccgggtttc gtcgttgagc
      601 ggcacgtgca ggctgacgag atcggactgc tcgaacaggt cggccagcgt cgcgcgctcg
      661 acatatgccg ggaaatcgcc gtcgatcaga tagggatcgc aagcgacgac gcgccggaag
      721 acgttgcgag acacatgcgc catgcgcttg ccgatacggc ccaggccgac gatgccgagc
      781 gtgagttcgc tggcgcggcg cagcctcccc gacgacagat aatgccagcg cccgccggcg
      841 atatcgcggt tataggcgac gacgttgcga atcagcgcga gccccagcgc cagggcatgc
      901 gtggccactt cgccgacgcc gtaatcgggc gagttcgcaa gccacacacc gtatttcgcg
      961 caggcgtcgg tgtctatcgt gtcgtagccc gcgccaatgc ggctgatgat gcccagccgc
     1021 ggcagctgcg caagcgcgcg tgcgccgatc ggtgcatact ggaccaagaa ggcatcgaag
     1081 ttccggcccg ccgcgagcac gtcgtcttcg gtgctgcact gggcaacggc aagctcgata
     1141 ccggcgttgc cgaacagctc gcgttcgagc gccaggtctt cgtaattctg gtcggtgatc
     1201 agggcgcggg ccatcgggat ccggcagatg ctgacgagcg ctgggaggcg ctccgggccg
     1261 caaacggtac gtcggtatcg accattggtc aagtggcctt accgcttgat ccacccctat
     1321 aatcgttctg cgccttcggg gctctccggg gcgctcacct ggaatccgcc gatgctgtta
     1381 ccaacgacga tcgccggcag cttgcccaag cccgcgtggc ttgcgccccc gaaacagctg
     1441 tgggcgccat ggctgctcga gggatcgcta ttggaggagg gcaagcgcga cgccgtgcgc
     1501 ctgg
//
LOCUS       DATANS010000746         2793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_832513, whole genome shotgun sequence.
ACCESSION   DATANS010000746 DATANS010000000
VERSION     DATANS010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832513"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..823)
                     /locus_tag="VHT22_11105"
     CDS             complement(2..823)
                     /locus_tag="VHT22_11105"
                     /inference="COORDINATES: protein motif:HMM:NF022665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HEX3138151.1"
                     /translation="MARLVWVVLALVVLAALAYFLWQKNQSREAIPPVASAPAEVAPP
                     AADAAPAINHPIEQARGNVPPSVEQKPLPALMVSDTTMQNTLADLFGSATLGRIFYED
                     AIVHRFVTTVDNLPRKTLPLRYLPVKPPGGAFVTTGSDDALAIGADNAARYTPYVRLI
                     DAVDAKTLAGIYVHFYPLMQQDYRALGYPNGYFNDRLVQAIDDLLAAPDLTAPPALAQ
                     PKVLYVYTDPELESRSAGQKIMMRMGSANAARVRAKLREIRAEITASGNTDLRKK"
     gene            complement(1081..>2793)
                     /locus_tag="VHT22_11110"
     CDS             complement(1081..>2793)
                     /locus_tag="VHT22_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HEX3138152.1"
                     /translation="HLLFALTLVFLLYPVKPRGAAAWRRIDLVLLALGYGFVLHIFTN
                     YEYFINRIIYIDDLTIADKFYAVIAVVIVLEATRRVIGLALPLTAVAFLIYAIGFTRI
                     TPQVLMEQLYFSTEGIFGSTLGVSASYVMLFVLFGSFMERSGTGQLFMDFSMSLTGHT
                     AGGPGKVAIVSSALFGTVSGSAVANVMVDGPITIPLMKRTGFRPPFAAAVEAVASTGG
                     QIMPPVMGAAAFVMAEFLAVPYAQVALWAAIPALLYYVSLFFAVHFDAKRYKLAGVPK
                     SELPRFLKVMSVRGHLFVPIFVVLIGLILGYSAPLCALAGALACIPMALLRASTRGGI
                     GWRNVFDALVDGARNTLSVAMACACAGIVIGCVTITGLGITFTEFVIVLAQSSLIAAL
                     VLTAIAGIVLGMGMPTTPSYIVMVSLLVPAIIKLGAIAPAAHMFAFYFAILSAITPPV
                     ALAVFAAAGLAKSDLWATGWQAVRIAAATYIVPFMFIYEPSLMLIGDWFESVTSTVSA
                     VIGVMCLAAGLQGYLLREARWWERAVLVVAALLLIKPGYISDAIGVVLLAAVLFSQKL
                     VPAK"
BASE COUNT          563 a          902 c          906 g          422 t
ORIGIN      
        1 gctacttttt cctgagatcc gtgttgccgg acgcggtgat ctccgcgcgt atttcgcgca
       61 gcttggccct gacccgcgcg gcattcgcgc tgcccatgcg catcatgatc ttctggccgg
      121 ccgagcgcga ttcgagctcg gggtcggtat acacgtacaa gacctttggc tgcgccagcg
      181 ccggcggcgc cgtcaggtcg ggcgcggcga gcagatcgtc gattgcctgc accagacggt
      241 cgttgaaata cccgttcggg tagccgagcg cgcgatagtc ctgctgcatc agcgggtaga
      301 agtggacgta gattccggcc agcgtcttcg catcgaccgc atcgatcagc cgcacatacg
      361 gtgtgtagcg cgcggcattg tcggcgccga tggccagcgc gtcgtcgctg ccggtagtga
      421 cgaacgctcc gcccggcggc ttgacaggca ggtagcggag gggcagcgtc ttgcgcggca
      481 gattgtcgac cgtcgtgaca aaacggtgga cgatcgcatc ctcgtaaaat atccggccga
      541 gcgtcgccga gccgaacaga tcggccagcg tgttctgcat tgtggtgtcg ctgaccatca
      601 gcgcgggaag tggcttctgc tcgaccgacg gcggtacatt ccctcgcgcc tgttcgatcg
      661 gatgattgat cgccggcgcg gcgtccgccg ctggcggcgc cacctctgcc ggcgcactgg
      721 cgacaggcgg gatcgcttct ctcgactggt tcttctgcca gaggaaatag gcgagcgccg
      781 ccagcacaac gagcgcaagc acgacccaga ccaggcgcgc cactccgtcc cggcgacggg
      841 cgcgaatcgg tcgcgcatcg gagtcggcgg cgagcgcggg cgcatcggcg gtcgaatgga
      901 aggccggatc aagccgatga gcttcgtccg tcgccgatcg agacgccatg atcattctcc
      961 cattgtagac gcatgctcgg tgccgcatcg accggcgcca gtgcgttggg ccgcgcgccg
     1021 cgggggaagt tcgccgccgc accgccgtgt cgttgctgcc cgacaatacg gggcggcccg
     1081 ttatttcgcc ggaaccagct tttgggaaaa cagcaccgcc gcgagaagca ctaccccgat
     1141 cgcatcgctg atatagcccg gcttgatcag caacagagcg gcgacgacca gaaccgcgcg
     1201 ctcccaccac cgcgcttccc gcaacagata gccctgcagg ccggccgcca ggcacatgac
     1261 gccgatcacc gcggacaccg tcgaagtgac gctttcgaac cagtcgccga tcagcatcaa
     1321 cgatggctcg tagatgaaca tgaaaggcac gatgtaggtc gccgccgcga tgcggacagc
     1381 ctgccagccg gtcgcccaca aatcgctctt cgcgaggccc gctgcggcaa acaccgccag
     1441 cgccaccggc ggcgtgatcg ccgacaggat cgcgaaatag aacgcgaaca tgtgcgctgc
     1501 gggcgcgatc gcgcccagct tgatgatcgc cggcaccagc agcgacacca tgacgatgta
     1561 cgacggcgtc gtcggcatgc ccatgccgag gacgatgccc gcgatcgccg tcagcaccag
     1621 cgccgcgatc agcgagctct gcgcgagtac gatgacgaat tcggtgaaag tgatgccgag
     1681 tccggtgatc gtcacgcagc cgatgacgat gcccgcacag gcgcaagcca tcgcgaccga
     1741 aagcgtgttg cgcgcgccgt cgaccagtgc gtcgaatacg ttgcgccagc cgattccgcc
     1801 acgggtgctg gcgcggagca aagccatcgg gatgcaggcc agcgctccgg cgagagcgca
     1861 cagaggcgcc gaatagccga ggatcaatcc gatcaggacg acaaagatcg gcacgaacag
     1921 gtgtccgcgc accgacataa ccttcaggaa tcggggcagc tcggacttcg gcacgccggc
     1981 cagcttgtag cgcttggcat cgaagtgaac ggcgaagaac agagagacgt aatacagcag
     2041 cgccggaatc gccgcccaca gcgcgacctg cgcatacggc acggcgagga actcggccat
     2101 cacgaacgcg gcagcaccca tcaccggagg catgatctgg ccgccggtcg atgcgacggc
     2161 ctcgaccgca gcggcgaacg gcggacggaa gccggtgcgc ttcatcagcg gtatggtgat
     2221 cggcccgtcg accatgacgt tggccaccgc gctgccggac acggtgccga acagcgccga
     2281 cgaaacaatc gcaaccttgc cgggtccgcc ggccgtgtga ccggtaagcg acatcgagaa
     2341 atccatgaac agctggccgg tgccgctgcg ctccatgaac gagccgaaca gcacgaacag
     2401 catcacatag cttgccgaga cgcccaacgt cgacccgaag atgccttcgg tcgagaaata
     2461 gagctgctcc atcagcactt gcggcgtgat ccgggtgaag ccgatcgcat agatcaggaa
     2521 ggccacggcg gtcagcggca gcgccaggcc tatgacgcgc cgcgtagcct cgaggacgat
     2581 caccaccgcg attaccgcgt agaacttgtc ggcgatcgtc agatcgtcga tgtagatgat
     2641 gcgattgatg aaatattcgt aattggtgaa gatgtgcagc acgaagccgt agccgagcgc
     2701 aagcagcacg agatcgattc tgcgccaggc cgcggcgccg cgcggcttga ccggatagag
     2761 caggaatacc agcgtcagcg cgaaaagaag gtg
//
LOCUS       DATANS010000747         1930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_583128, whole genome shotgun sequence.
ACCESSION   DATANS010000747 DATANS010000000
VERSION     DATANS010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583128"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1735
                     /locus_tag="VHT22_11115"
     CDS             <1..1735
                     /locus_tag="VHT22_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138153.1"
                     /translation="SANIVPAEGSPLEMFGFGKSIKDPLADAKTAERWLSTFPANDPL
                     AAHAAILTELGALTERQAHRAPARLEAVFRLDVQSEALRRTLTSQYHEHGTRSSRVES
                     QLWQALFDLTQGFLLCYQAFAREVSDHAQSNRWRALLPELIARQIIHHGLDAKIRLYR
                     YEQWIPARWSELHSLFQIACSIELERQPIAVLADGTLTTIEQEYLRVLLLQLMNAGNL
                     SARHVEWVAEQLSEWCAPLRLNVESSTATSFYVDLGARSGLKRRAPGPLEGRVLFLDT
                     RPLHAMLMQNVVMLEQKVRSDPLSDRTLRRTEQLNLMTKLAAQVDPEFRPVPRRGERI
                     NASGAVDAIVGFTKIAGYLHDEEVHPLAEKRHRTGNFDDSIELATFGHMRNENARVQE
                     VARRLLAAYAAPGGAWDIRDVSHTGYRLVAPMSVINSVTLGTLAAIRAHGQPLWTLGI
                     VRRMKRLTSERAEIGLQVIANNLVGVELFEQKRGDADYSVDGVVPTVSGRRFPGLFLS
                     LRRRDSEATIQTLIVPAGEYQPGKRMQMRVARVAQQIAFGRLLEQQPEWIWATIESQD
                     NSARAAAATAA"
     gene            1749..>1930
                     /locus_tag="VHT22_11120"
     CDS             1749..>1930
                     /locus_tag="VHT22_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3138154.1"
                     /translation="MTVDRYRELCARHRWNVPHDFNIAAAVCGRHASDGARVAIHWED
                     ESGATTTLSFGELQRQA"
BASE COUNT          345 a          624 c          654 g          307 t
ORIGIN      
        1 tagtgcaaat atcgtaccgg ccgaaggaag cccgctcgaa atgtttggtt tcggcaagtc
       61 gatcaaggac ccgctggcgg acgcaaagac cgccgagcgg tggctttcga cctttccggc
      121 caacgatccg ctggcggcgc acgcggccat cctgaccgag cttggcgctc tgaccgagcg
      181 ccaagcccat cgcgcgccgg cgcggctcga ggccgtgttc cggctggacg tccagagcga
      241 ggcgctgcgc cgcacgctga cctcgcagta tcacgagcac ggaacccgct cgagccgcgt
      301 cgaaagccag ctttggcagg cgctgttcga cctgacccag ggctttctgc tgtgctacca
      361 ggcgttcgcg cgcgaagtca gcgaccacgc ccagagcaac cgatggcgcg cgctccttcc
      421 cgagctgatc gcgcggcaga tcatccacca cggcctcgac gccaaaatcc ggctgtatcg
      481 ctacgagcag tggattccgg cgcgctggtc cgagctgcat tcgctgttcc agatcgcctg
      541 ttcgatagag ctcgagcggc aaccgattgc cgttctggcc gacggcacgc tgacgacgat
      601 cgagcaggaa tatttgcgcg tcctgctgct gcagctgatg aacgcaggca acctctcggc
      661 gcggcacgtc gaatgggtgg ccgagcagtt gtccgagtgg tgcgcgccgc ttcgcctgaa
      721 cgtcgaatcg tcgacggcga cgagcttcta cgtcgacctg ggcgcccgca gcggattgaa
      781 gcgtcgcgcc cccggaccgc tcgaagggcg cgtgctcttc ctggacacgc ggccgctgca
      841 tgcgatgctg atgcagaacg tcgtcatgct cgagcagaag gtgcgcagcg acccgctgtc
      901 cgaccggacc ttgcgccgca ccgagcagct caacctgatg accaagctcg ccgcgcaggt
      961 ggatccggag ttccggccgg tgccgaggcg cggtgaacgc atcaatgcgt cgggagcggt
     1021 cgatgcaatc gtaggtttca ccaagatcgc cggctacctg cacgacgagg aagtgcaccc
     1081 gctggccgaa aagcggcatc gcacggggaa tttcgacgat tcgatcgagc tcgcgacctt
     1141 cggccacatg cgcaacgaaa atgcacgggt gcaggaagtc gcgcggcggc tgctggcggc
     1201 ctatgcagcg cccggcggcg cctgggatat acgcgatgta tcgcacaccg ggtaccggct
     1261 ggtcgcgccg atgagcgtga tcaactcggt caccctggga acgctggccg cgatccgcgc
     1321 gcatggccag ccgctatgga cgctcggcat cgtgcggcgg atgaagcgac tgaccagcga
     1381 gcgcgccgag atcgggttgc aggtgatcgc caacaatctg gtcggcgtcg agttgttcga
     1441 gcagaagcgc ggcgacgccg actattcggt tgatggcgtc gtgccgacgg tcagcggtcg
     1501 ccgcttcccg ggactattcc tgtcgctgcg ccggcgcgac agcgaggcaa cgatccagac
     1561 cttgatcgtg ccggcgggcg aatatcagcc gggcaagagg atgcagatgc gcgtcgccag
     1621 agttgcgcag cagatcgcat tcggtcgcct gctcgaacag caaccggaat ggatctgggc
     1681 gacgatcgaa tcgcaggaca actcggcacg cgccgccgcc gcgacggcgg cctgaagccg
     1741 cgcgccggat gacggtcgat cgctatcgcg agctttgcgc gcgacatcgc tggaatgtgc
     1801 cgcatgactt caacatcgcg gccgcggtat gcgggcgcca cgcgagcgac ggagcgcgtg
     1861 ttgcgatcca ctgggaagac gagtccggcg cgacgacgac gctgagtttc ggcgagctgc
     1921 agcggcaggc
//
LOCUS       DATANS010000748         1755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_583214, whole genome shotgun sequence.
ACCESSION   DATANS010000748 DATANS010000000
VERSION     DATANS010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583214"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VHT22_11125"
     CDS             <1..448
                     /locus_tag="VHT22_11125"
                     /inference="COORDINATES: protein motif:HMM:NF037181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138155.1"
                     /translation="LHPRLLALALPRVLRAVRGKDAGDLERRFQAWIRPEPAARAELA
                     YAAIVAGLEQEPFVRDCLPDLLREDPSLGEIVRSVAGDPGQLTQQQRTDLALGLARIL
                     RRTQRYRCVDCGFASASHFWQCPGCRSWDAFAPVALLDVTPGMQRR"
     gene            568..858
                     /locus_tag="VHT22_11130"
     CDS             568..858
                     /locus_tag="VHT22_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX3138156.1"
                     /translation="MSKLIAALIVTLMPALVWAGPVDINTADAATIAKELQGIGPNLA
                     QAIVAYREKNGAFRNADELRKIKGIGAKVLEKNRANIKLDGKAEGAPRKKTE"
     gene            916..>1755
                     /gene="galU"
                     /locus_tag="VHT22_11135"
     CDS             916..>1755
                     /gene="galU"
                     /locus_tag="VHT22_11135"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX3138157.1"
                     /translation="MTQAREIRTAVFPVAGRGTRFLPATKASPKEMLPIVDKPLIQYA
                     AEEALAAGARKLVFITGSSKRAIEDHFDSDAELEAALEAQGKADLLKTLKDIVPAWAS
                     CIYIRQPSPLGLGHAVWCAKPVVGDEPFFVHLADDLIDGPTPCLRQMAAVHAERGGSV
                     LGVEQVPRSETDKYGIVATDNREGRVSLVKQIVEKPRPQNAPSTLAVVGRYLLSPTIF
                     AKLEQTGRGAGGEIQLTDAIADLLADEPVYAYEFEGKRYDCGSKLGYLQATVEYGLKH
                     EALG"
BASE COUNT          312 a          551 c          589 g          303 t
ORIGIN      
        1 gctgcatccg cgtctgctcg cgctcgcctt accgcgtgtg ttgcgtgcgg tacgtggcaa
       61 ggacgcaggc gatctggagc ggcgtttcca ggcatggatt cggccggagc cggccgctcg
      121 cgccgagctc gcgtacgccg cgatcgtcgc gggtctggag caggaaccgt tcgtgcgcga
      181 ttgcttgccg gacttgctgc gcgaagatcc gagtctcggc gagatcgtcc gctcggtcgc
      241 cggcgatccg ggtcaactga cgcagcagca gcgcacggat ctggcgctgg ggctcgcgcg
      301 catcctgcgt cgaacgcaac gttatcgctg cgtcgactgc ggcttcgcga gcgcctcgca
      361 cttctggcaa tgccccggtt gccgcagttg ggatgccttc gcgccggtcg cattgctcga
      421 cgtcactccg ggcatgcagc ggcgctgatt ttctgcgcga tttgagtagt tattaccgct
      481 caccgctctc ttgcgccggc gcatcctgat cgcgccgacc ccggtaattg tttcaacacc
      541 tggttgtttc atagcaagga gagcttcatg tccaaactca ttgccgccct catcgtcacg
      601 ctcatgccgg cgctcgtctg ggcgggtccg gtcgacatca acactgcgga tgccgccacc
      661 atcgccaagg agctgcaggg catcggcccg aatctcgcgc aggccatcgt tgcatacaga
      721 gaaaagaacg gcgctttccg caatgccgac gagctgcgca agatcaaagg catcggcgcc
      781 aaggtgctgg agaagaaccg cgccaacatc aagctggacg gcaaggccga aggcgcgccg
      841 cgcaagaaga ccgagtaggg cgctgacggt cttccgggta tcatcgcgct cctgcgaaca
      901 cgacggaggt gcgcggtgac ccaggcgcga gagatcagaa cggcagtttt tccagtggcc
      961 ggacgcggta cgcggttctt gcccgcgacc aaggcaagcc cgaaagagat gctgcccatc
     1021 gtcgacaagc cgctgatcca atatgccgcg gaggaagcgc tcgctgccgg tgcacgcaag
     1081 ctggtgttca tcaccggcag ctcgaagcgc gcgatcgaag atcacttcga cagcgacgcg
     1141 gagctggagg cggcgctgga agcgcagggc aaagccgacc tgttgaagac gctgaaggac
     1201 atcgtgccgg cgtgggcgag ctgcatttac atccggcagc cgtcgccgct cggtctcggt
     1261 cacgctgtgt ggtgtgccaa gccggtcgtc ggcgacgagc cgttcttcgt gcatctcgcg
     1321 gacgatttga tcgacgggcc gacgccgtgc ctgcgtcaaa tggcggcggt gcacgcggag
     1381 cgcggcggca gcgtgctcgg tgtcgaacaa gtaccgcgtt ccgagacgga caagtacggc
     1441 atcgttgcga ccgacaatcg cgaagggcgc gtcagcctcg tgaagcagat cgtcgagaag
     1501 ccgcggccgc agaatgcgcc gtcgacgctg gctgtcgtgg gtcgctacct gttgtcaccc
     1561 acgatcttcg cgaagctgga acagaccggc cgcggcgcgg gcggcgagat tcagctcacc
     1621 gatgcgatcg ccgacctgct ggcggacgag ccggtgtacg cgtacgagtt cgaaggcaag
     1681 cgttacgact gcggcagcaa gctcggctat ctgcaggcga ccgtcgagta cggtctgaag
     1741 cacgaagcgc tcggc
//
LOCUS       DATANS010000749         1588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_332886, whole genome shotgun sequence.
ACCESSION   DATANS010000749 DATANS010000000
VERSION     DATANS010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332886"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VHT22_11140"
     CDS             <1..399
                     /locus_tag="VHT22_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3138158.1"
                     /translation="LRDFSNDVYFQIGLLVLIGLAAKNAILIVEFAAQELAKGLSPVD
                     AALEAARLRFRPIVMTSLAFMLGVLPLFLATGAGAAARRSMGTGVFGGMVVATFVATI
                     FIPLFFVLLRRRKDKDKPAPAPAGVEERTA"
     gene            399..>1588
                     /locus_tag="VHT22_11145"
     CDS             399..>1588
                     /locus_tag="VHT22_11145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX3138159.1"
                     /translation="MHRRLAMAVALALAGCMVGPDYQRPQTTLPTTYAGAPVVQANAD
                     SAVAIRVDWWTLYDDRMLNDLVAAALSDNLDVAAAVARIEEFDADLREANAALFPELD
                     LGASAARSRSSGAVAAPQSIRVSNDLRIALSTSFEIDFWGRLRRLLESARAQVLATHY
                     AKDVVTLSLAGLTSQTYFLLRSLDAQVTATRETLATREEYLVVVRRRVDAGLASGLDL
                     AQALGARSDAAAQLKELVRQRDLAEHLLGTLTSRLDLKVAPGDLAQLPFPPTPPMDLP
                     STLLERRPDIRLAEENLVAANAQIGVAKAALLPRISLTGLLGGESAELSSLARSSAGI
                     WSIGFALAQPIFTWGRLTAEVDATTARQKQALVGYQKSIQTAFREVSDALVNVGQTSA
                     MEIDL"
BASE COUNT          240 a          525 c          538 g          285 t
ORIGIN      
        1 ctgcgcgatt tcagcaacga tgtttacttc cagatcggat tgctggtgct gatcgggctc
       61 gccgccaaga atgcgatcct gatcgtcgaa ttcgccgcgc aggagctcgc gaagggcctg
      121 tcgccggtcg acgccgctct cgaggcggcg cgattgcgct tccgtccgat cgtgatgacc
      181 tcgctggcgt tcatgctcgg ggtcttgccg ctgtttctcg ccacgggcgc cggtgcggcg
      241 gcacggcgtt cgatgggcac cggcgtgttc ggcggaatgg tcgtggcgac attcgtcgcc
      301 accattttca ttccgctgtt cttcgtgctc ctcaggcgac gcaaggacaa agataaaccc
      361 gcgccggcgc cggccggagt cgaagaaagg accgcctgat gcatcgccgc ctggcgatgg
      421 cagttgcgct ggcgcttgcc ggctgcatgg tggggcccga ctatcagcgg ccacagacca
      481 cgctgccgac gacctacgcc ggcgcgccag tggtccaggc caatgccgac agcgctgtgg
      541 cgatacgcgt cgattggtgg acgttgtacg acgaccgcat gctcaacgat ctggttgcgg
      601 ccgcgctgtc ggacaatctc gatgtcgccg ccgccgtggc gaggatcgag gagttcgacg
      661 ccgatctgcg cgaagccaat gcggcgttgt ttcccgaact cgatctcggc gcaagcgccg
      721 cgcgctcgcg ctcgagcgga gccgtcgcgg cgccgcagtc gatcagggtg agcaacgatc
      781 tgcgcatcgc attgtcgacg tctttcgaaa tcgatttctg gggacggctg cgccgactgc
      841 tcgagagtgc gcgggcgcag gtcctggcga cccattacgc gaaggacgtg gtcacgctgt
      901 cgctggcggg gctgacgtcg cagacgtatt tcctgctgcg ctcgctcgac gcgcaggtca
      961 cggccacccg cgaaacgctt gcgacccgcg aggagtacct ggtcgtcgtt cgccgccgcg
     1021 tcgacgccgg ccttgcctcg ggtctcgatc tcgcccaggc gctgggcgcc cgctcggacg
     1081 ccgccgccca gctgaaggag ctcgtgcggc agcgcgacct ggccgagcat ctgctgggga
     1141 cgctcacctc gaggctcgat ctcaaggtgg cgcctggcga tctcgcgcag ttgccctttc
     1201 caccgacacc gccgatggac ttaccgtcga ctctgctgga acgacggccg gatatccggc
     1261 tggccgagga aaacctggtc gcggcgaacg cgcagatcgg cgtcgccaaa gcggcgctgt
     1321 tgccccggat ctcgttgacc ggcctcctcg gcggcgaaag cgccgagctc agctcgctcg
     1381 cacgcagcag cgccggcatc tggtcgatcg gatttgcgct ggcgcagccg atcttcacgt
     1441 ggggccgcct gaccgcggaa gtcgacgcca cgactgctcg gcagaagcag gcgctcgtcg
     1501 gctatcagaa gtcgattcag acggcgttcc gcgaggtttc cgacgcgctg gtgaacgtcg
     1561 gccagacgtc ggcgatggag atcgatct
//
LOCUS       DATANS010000750         4248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_373944, whole genome shotgun sequence.
ACCESSION   DATANS010000750 DATANS010000000
VERSION     DATANS010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373944"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VHT22_11150"
     CDS             complement(<1..309)
                     /locus_tag="VHT22_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3138160.1"
                     /translation="MTALLEVRDLRTLFAVEAGGEFAAVDGVGFSLDAGRTLGIVGES
                     GCGKSVTALSIMGLVAQPPGRIGGGSILFDGVDLLRLPPSAMRELRGDRISMIFQEPM
                     T"
     gene            complement(306..1019)
                     /locus_tag="VHT22_11155"
     CDS             complement(306..1019)
                     /locus_tag="VHT22_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX3138161.1"
                     /translation="MTWSIVARDADGAFGVAVASRFFAVGALCPHARSGIGALSTQAL
                     VNPGYGPSGLDLLGRGVAATDTVARLVAGDEGREHRQLHLIDRRGRCAAHTGTQCIDW
                     CGDRAHDGFSVAGNMLAGPAVLDETARAYQAHAGAPFAERLLAAMAAGEAAGGDKRGK
                     QAAALLIHTTETYAFLDLRVDDHPDPIAELKRLYQVSLERYQPFLACLPSAARPAGIT
                     DRAAIDAEVERFQAERRQR"
     gene            complement(1060..1947)
                     /locus_tag="VHT22_11160"
     CDS             complement(1060..1947)
                     /locus_tag="VHT22_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX3138162.1"
                     /translation="MTTSTRNLAVLFADVSGSTKLYEMLGNADAQQAIDRCLAIVKRV
                     CSDTGGNVVKTIGDEVLATFPTSDAAAQAAIEIQMRVASQRTSRGTGLSMHIGFHAGP
                     TIEDAGDVFGDAVNVAARMTSFAAGGQIFTTAQTVAELSPVLRGRTRDHAAQTVKGKE
                     HDIGLFELLWQDSEDELTAMSPRAALAPAMVKLVHGLRKIELGEAHSVLTLGRDLQSD
                     VVIADRKASRMHARIERRRDKFVFIDHSSNGSWISIEGEPEIMIRREELMLRGRGRIS
                     FGHAHADDPSEFLGFSCLD"
     gene            2111..3007
                     /locus_tag="VHT22_11165"
     CDS             2111..3007
                     /locus_tag="VHT22_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138163.1"
                     /translation="MKRSLTWLAGFLFLIAVPCFGAETATVTLLFGQPKLLRGTTWFN
                     LKEGVRLRDGDVIDLPASGQLQLEFTDTGAVSVIGPGALYIIAASARDAKQAGSAEFQ
                     LTRGWLKFDTKPPAPRLRVRTALGTIAASDAAAVIHAGDGFEAFVETGTAKLSDVGKL
                     ELTGGEIKAGGFASRSAGKPLTAERRPPAAFVGALPRDFMDPLPVRADRFSARVEPVA
                     DHESTFAEAQPWLNGPYRASFLKRFQARLDDPAFRDAMATSGKAPPEWSAAAPPPKVE
                     TPPPAPAAKVEPERKFRWPWEK"
     gene            3027..3905
                     /locus_tag="VHT22_11170"
     CDS             3027..3905
                     /locus_tag="VHT22_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEX3138164.1"
                     /translation="MKLIWKFNLAILAVAAVAFILAAVVSYTVLHANARDEVLQNARV
                     MMESANASGDYTNAQVRPLLETQLKYKFIPQSVPAFAATEQFNELRKNYPDYTFKAAT
                     LNPTNPRNHATDWEIDIVNQFRQSAAKTEVIGERASQTGRSLYLARPIQIKDPACLTC
                     HSTPDAAPRTMVETYGSANGFGWKVNEIVGANIVSVPTSVPVQRANYTFRIFLLSLLV
                     VFAFTIGMLNVMLYLFVIRRVKRLSKLADEVSLGNLDAGEFQTRSKDEIGVLTEALGR
                     MKTSMVQAIKMLDAHG"
     gene            3938..>4248
                     /locus_tag="VHT22_11175"
     CDS             3938..>4248
                     /locus_tag="VHT22_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016977394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3138165.1"
                     /translation="MTEPTRIGKYTIESVLGKGAMGVVYKAFDPGIERVVAIKTVRKD
                     RVDQDLIDQSMARFKNEAKAAGRLLHPNIVSVYEYGEDEANAFIVMEFVEGTGLREFI
                     NR"
BASE COUNT          737 a         1397 c         1416 g          698 t
ORIGIN      
        1 ggtcatcggt tcctggaaga tcatcgagat cctgtccccc cgcaactcgc gcatcgccga
       61 aggtggcagc cggaggaggt cgacgccgtc gaacaggatg ctgccgccgc cgatccgtcc
      121 gggcggttgc gcgaccaggc ccatgatcga cagggcggtg acgcttttgc cgcaaccgga
      181 ttcgccgacg atgcccagtg tgcgtccggc gtccagcgag aagccgacgc cgtcgaccgc
      241 cgcgaattcg ccgccggctt cgacggcgaa cagcgtgcgc aaatcccgca cttcgagcag
      301 cgccgtcatc gctgccggcg ctctgcctgg aaacgctcga cttcggcgtc gatcgccgcg
      361 cgatcggtga tgccggcagg gcgtgccgcg ctcggcaggc aggcgaggaa cggctgatag
      421 cgctcgaggc tcacctgata caggcgtttc aattcggcga tcggatccgg gtgatcgtcg
      481 acgcggaggt cgagaaacgc ataggtctcg gttgtgtgga tcaacagcgc tgctgcctgc
      541 ttgccgcgct tgtcgccgcc tgccgcctcg cccgccgcca tcgcggccag caatcgctcg
      601 gcgaacggcg cgccggcgtg tgcctggtag gcgcgcgcgg tctcgtcgag cacggctggg
      661 cccgcgagca tattgccggc caccgagaac ccgtcgtgcg cgcgatcgcc gcaccagtcg
      721 atgcactgcg tgccggtatg ggcggcgcag cgaccgcgcc gatcgatcag gtggagctgg
      781 cgatgctcgc ggccttcgtc gcccgccacc agccgtgcga cagtatcggt cgcggcaacg
      841 ccccgcccga gcaggtcgag tccggacgga ccgtagccgg gattgaccag cgcctgcgtc
      901 gacagcgcgc cgatgccgct gcgcgcgtgc ggacaaaggg cgccgaccgc gaaaaagcgg
      961 ctcgctacgg caacgccgaa cgccccatcc gcatcgcgcg cgacgatcga ccaggtcacg
     1021 gcagcctacg cgcgcgcggc cgcggcgatg cgaagcaggt caatcgagac aggaaaaacc
     1081 gagaaactcg gaggggtcgt cggcgtgggc atgcccgaag ctgatccgcc cgcggccgcg
     1141 cagcatcagc tcctcgcggc ggatcatgat ctcgggttcg ccttcgatgc ttatccagct
     1201 gccgttcgag ctgtggtcga tgaatacgaa tttatcgcgg cggcgctcga tgcgtgcgtg
     1261 catgcgcgat gccttgcggt cggcgatgac gacgtcgctc tgcagatcgc gacccagcgt
     1321 cagcaccgaa tgcgcctcgc ccagctcgat cttgcgcaga ccgtgcacca gcttgaccat
     1381 cgccggcgcc agcgcggcgc gcggcgacat cgcggtgagc tcgtcttcgg agtcctgcca
     1441 caggagctcg aacaggccga tgtcgtgctc cttgcctttg accgtctgcg cggcgtggtc
     1501 gcgcgtgcgg ccgcgcagga ccggcgagag ctcggcgacc gtttgcgcgg tcgtgaagat
     1561 ctggccgccg gcggcgaacg atgtcatgcg tgcggcgacg ttgaccgcgt cgccgaagac
     1621 gtcgccggcg tcctcgatcg tcggaccggc gtgaaagccg atatgcatag acagtcccgt
     1681 gccgcggctg gttcgctgcg aggcgacgcg catctggatc tcgatcgcgg cctgcgccgc
     1741 cgcgtcggag gtcgggaacg tggcgaggac ttcatcgcct atcgtcttga cgacgttgcc
     1801 tccggtgtcg ctgcacacgc gcttgacgat cgcaagacag cggtcgatcg cctgctgtgc
     1861 gtcggcgttg ccgagcattt cgtacagctt ggtgctgccg gaaacgtcgg cgaacaggac
     1921 ggcgaggttg cgcgtggagg tcgtcatgcg gcgattgcac gtcggatcgt cgcaagtata
     1981 aggcatgaca tcggctgcga cccccgccag ccttggcttg ccgagtattc acgttgactt
     2041 gacgggggtg ccggcctagt atgccccgta cgtcgccacc gcttcgccca tcggcctgac
     2101 ggaccccgta atgaaacgat ctttgacctg gcttgccggt tttttattcc tgatcgccgt
     2161 cccctgtttc ggcgcggaga ccgccacggt cacgctgctg ttcggccagc ccaagctgct
     2221 gcgcggcacg acctggttca acctgaagga aggggtgcgg ctgcgcgacg gtgacgtcat
     2281 cgacctgccg gcatcggggc agttgcagct cgaattcacc gacaccggcg ccgtcagcgt
     2341 catcggccca ggcgcgcttt acatcatcgc cgcctcggcg cgcgacgcca agcaggccgg
     2401 gtcggcggaa ttccagctca cccgcggctg gctgaaattc gacaccaagc cgccggcacc
     2461 gcgccttcgc gtacgcaccg ctctgggcac gatcgcggcc tcggatgccg ccgcagtcat
     2521 tcatgccggc gacggattcg aagcctttgt cgagaccggg accgccaagc tgtcggacgt
     2581 cggcaagctc gagctcaccg gcggcgagat caaggccggc gggttcgcaa gccgctccgc
     2641 aggcaagccg ttgaccgcag agcggcgccc gccggcagcg ttcgtcggcg cgctgccgcg
     2701 cgacttcatg gatccgttgc cggtacgcgc cgaccgcttc tcggcgcgcg tcgagccggt
     2761 tgcggatcac gagtcgacgt tcgccgaggc gcagccgtgg ctgaacggcc cgtaccgcgc
     2821 cagcttcctc aagcgcttcc aggcgaggct cgacgacccg gctttccgcg acgcgatggc
     2881 gaccagcggc aaggcgccgc cggaatggag cgccgcggcg ccgccgccca aggtggaaac
     2941 gccgccgccg gccccggccg ccaaggtgga gccggagcgg aaattccgct ggccgtggga
     3001 aaaataaacc gaacaggcgg acacacttga agctgatctg gaaattcaat ctcgccatac
     3061 tggcggtcgc ggcggtcgcg ttcatcctgg cagcggtcgt ctcgtacacg gtgctgcacg
     3121 ccaacgctcg cgacgaagtg ttgcagaacg cgcgcgtgat gatggagtcg gccaatgcat
     3181 ccggcgatta cacgaacgcc caggtcagac ccttgctcga aacgcagctc aagtacaagt
     3241 tcatcccgca atcggtgccg gcgttcgccg cgaccgagca gttcaacgag ctgcgcaaga
     3301 actatccgga ctacacgttc aaggcggcga ccctgaaccc gaccaatccg cgcaatcacg
     3361 cgaccgactg ggaaatcgac atcgtcaacc agttccggca gtcggcggcc aagaccgagg
     3421 tgatcggcga gcgggcgtcg cagaccggtc gttcgctcta ccttgcccgg ccgattcaga
     3481 tcaaggatcc cgcgtgtctg acctgccaca gcacgcccga cgcggcgccg cggacgatgg
     3541 tcgagaccta cggatcggcc aacggcttcg gctggaaggt caacgagatc gtcggcgcaa
     3601 acatcgtttc ggtgccgacg tcggtgccgg tgcagcgtgc gaattatact ttcaggattt
     3661 ttctgctctc attgctcgtc gtattcgcgt ttacgatagg catgctaaac gttatgctct
     3721 acttgttcgt catccgtcgc gtcaagcgac tgtccaagct cgcggacgag gtgagcctcg
     3781 gcaacctcga cgcgggtgag ttccagacga ggagcaagga cgagatcggc gtgctgaccg
     3841 aagcgcttgg acggatgaag accagtatgg tgcaggcgat caagatgctg gatgcgcacg
     3901 gctagcgcat gccggcggaa cacgaatcga gctgaaggtg actgaaccta cccgtatcgg
     3961 caagtacacg atcgagagcg tgctcggcaa gggcgcgatg ggcgtcgtct acaaggcgtt
     4021 cgatcccggc atcgagcgcg tggtcgcgat caagacggtg cgcaaggacc gcgtcgacca
     4081 ggacctgatc gaccagtcga tggcgcgatt caagaacgag gccaaggcgg ccggccggtt
     4141 gctgcatccg aacatcgtca gcgtctacga atacggcgaa gacgaagcca acgcctttat
     4201 cgtgatggag ttcgtcgagg gcacgggact gcgcgagttc atcaatcg
//
LOCUS       DATANS010000751         2558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_832932, whole genome shotgun sequence.
ACCESSION   DATANS010000751 DATANS010000000
VERSION     DATANS010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832932"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2286
                     /locus_tag="VHT22_11180"
     CDS             <1..2286
                     /locus_tag="VHT22_11180"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3138166.1"
                     /translation="RGYRFNLPVTSDSPETQSSAAQNQVAGTAVSEVLEWEPRSNLSS
                     RLPLLYGRADDFAAILALLRRHTIVTITGSGGIGKTRVAQAVARQLLTESAERYPDGV
                     WWVELSALADGALVPSAIAEAMNISLPAERPTPQALGAELASRRALLVLDNCEHLAES
                     VALLLDVFAAGASHLSVLITSQETLRASDEHVYRLGGLAVPDALDVETASHAGAVELF
                     AARAQAADPRFALTPGNVPAVIEICRRLDGIPLAIELAVARLPLLGVEGLRARLHERF
                     NVLTGGARVVLRRHQTLRATLEWSHALLTPDEQTVFRRLGVFAGGFTLEAAQHVASDE
                     RIDPWTTLDYLGALVEKSLVLAEGDPIPRYRMLETTRAYALERLAHAGEMQASLLRHA
                     QALVALLEPLERHEWLWRGTADPGARGELDNLRAALEWTDNAGDGPLAVTLAGLSYSV
                     WWSSAHMAEGLARCLALRRHLHSGVSAKDAARFWLTVAELGLYSIRCEGYDAAIRAAA
                     LYRELGDDQRRFESLTHAAIQATRFGTLVEMQAQIEEAAGLERPEWPARQRGKLQFAR
                     CFWFARQSRFEEALACARRQVAICREAGVEVGALFGMSNVTFMEIMAGRPSEAAEHAA
                     EAIARLHAIGADAGAGHLYFARTVALLMLDRVDDALGAAQAAYPRLLSEGDHHRLLRP
                     LALIVALQGRLDAAARIAAFDDSIQLRSGENADVFAPPVGGRLDALLTGLTSEERARL
                     AREGASLDETHAFRLAFGEVT"
     gene            2455..>2558
                     /locus_tag="VHT22_11185"
     CDS             2455..>2558
                     /locus_tag="VHT22_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3138167.1"
                     /translation="MEPAHNPLFRSLWIATIVSNVGTWMQDVGAGWLMT"
BASE COUNT          375 a          874 c          887 g          422 t
ORIGIN      
        1 cgcggatatc gcttcaacct gccggtgact tccgacagcc ccgaaacgca gtcgtcggca
       61 gcccagaatc aagtcgcggg aaccgccgta tccgaagtgc tggaatggga gccgcgctcc
      121 aatctgtctt cgcggctgcc actgctctat gggcgtgccg atgacttcgc cgccatcctg
      181 gcgttgctgc ggcgccatac gatcgtcacc atcaccggat cgggtggcat cggcaagacg
      241 cgggtcgctc aagcggtcgc acgacagctt ctcaccgaat cggccgagcg ttatccggac
      301 ggcgtctggt gggtcgagct ctcggccctt gccgacggcg cgctggtgcc gtcggccatt
      361 gcggaggcga tgaacatttc gcttccggcc gaacggccga cgccgcaggc gttgggcgcg
      421 gaactcgcgt cgcgtcgcgc gctgctcgtc ctcgataatt gcgaacatct ggccgaatcc
      481 gtggccctgc tcctggacgt cttcgccgcc ggggcgtcgc acttgagcgt gcttatcacc
      541 agtcaggaaa cgctgcgcgc gagcgacgaa catgtgtatc gcctcggcgg gctggccgtg
      601 cccgacgcgc tcgacgtcga gacggcgtcg catgccggtg cggtggagct cttcgccgcg
      661 cgcgcgcagg ccgcggatcc gcggttcgct ctgacgcctg gcaatgtgcc ggcggtcatc
      721 gaaatctgcc gccgcctcga cggcattccg ctcgccatcg agctcgcggt ggcccgcctg
      781 ccgctgttgg gcgtcgaagg cttgcgcgcc cgcctgcacg agcggttcaa cgtgctgacc
      841 ggcggggcgc gcgtcgtcct gcgccgccat cagacgctgc gcgcgacgct ggagtggagc
      901 catgcgctgc tcacgcccga cgagcagacc gtgttccgga gactgggcgt attcgcaggc
      961 ggcttcacgc tcgaggcggc tcagcacgtc gcgagcgacg agcgcatcga tccatggacg
     1021 acgctggact acctcggcgc gctggtagag aaatcactgg tgctcgccga aggcgatccg
     1081 atcccgcgtt accggatgct ggagaccacg cgcgcttacg cactggagcg gctcgcgcat
     1141 gccggcgaaa tgcaggcgag cctgctccgc cacgcacagg cgctcgttgc cctgctggag
     1201 ccgctcgagc ggcacgaatg gctatggcgg ggaacggcgg acccgggcgc acggggcgag
     1261 ctcgacaatc tgcgtgcggc gctcgaatgg actgataacg ccggcgacgg gccgctggcc
     1321 gtgacgctcg cgggcctgtc gtacagcgtc tggtggtcgt cggcgcacat ggccgagggg
     1381 ctcgctcgct gcctcgcact gcgccgccac ctccacagcg gtgtatcggc gaaggatgca
     1441 gcgcgcttct ggctcactgt cgcagagctc ggcttgtatt cgatccggtg cgaaggttac
     1501 gacgccgcca tccgcgcggc ggcgctgtat cgcgagctcg gcgacgacca gcggcgattc
     1561 gagtctctga cccacgcggc gatccaggcc acgcgcttcg gaacgctcgt ggagatgcaa
     1621 gcgcaaatcg aagaggccgc ggggctcgaa cggcccgaat ggcccgcgcg ccagcgtggc
     1681 aagctgcagt tcgcgcgctg cttctggttc gcacgccaaa gccggttcga ggaggcgcta
     1741 gcgtgcgcgc ggcgccaggt cgcgatctgc agggaggccg gggtcgaagt cggagcgctc
     1801 ttcggcatgt ccaatgtcac gttcatggag atcatggcgg ggcgtccgag cgaggctgcc
     1861 gagcacgctg ccgaggcgat cgcccgtctc catgcgatcg gcgcggacgc cggcgccggc
     1921 cacctctact tcgcgcgaac cgtcgcgttg ctcatgctcg accgggtcga cgacgcgctt
     1981 ggcgccgcgc aagcggcgta tccgcgcctg ctgagcgaag gcgatcatca ccggctcctg
     2041 cggcctcttg cgctgatcgt cgcgctgcag ggccggctcg atgctgccgc gcgtatcgcc
     2101 gcattcgacg attcgatcca gctgcgcagc ggcgaaaatg cggacgtctt cgccccgccg
     2161 gttggcggcc ggctcgacgc tttgctcacc gggctcacaa gcgaggagcg tgcacggctc
     2221 gctcgcgagg gcgcgagcct cgacgaaacg catgcattca ggctggcatt cggcgaggtg
     2281 acgtagcagc cacaggctgg acggctcccc ggacggtgat gccggaccgc tgtgctagca
     2341 tcgctcgtcc gatttcggcc gccttcgatc caacgttgcc cgaccgcaag ctatgagacc
     2401 gtccccatcg ggcgcgcccg cgggtccagt cccgggtcgg gcggctccgt cggcctggag
     2461 cccgctcata acccgctgtt ccgaagcctg tggatcgcca cgatcgtgtc gaatgtcggc
     2521 acctggatgc aggacgtcgg cgccggctgg ctgatgac
//
LOCUS       DATANS010000752         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_793208, whole genome shotgun sequence.
ACCESSION   DATANS010000752 DATANS010000000
VERSION     DATANS010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793208"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VHT22_11190"
     CDS             <1..523
                     /locus_tag="VHT22_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013819973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX3138168.1"
                     /translation="HPDGPFSHLTWHAIVAARHHQNHGADKLDAAGSTAEFLTRAMRR
                     VIDEETARIEAGLTVLASVGSTAPFVGLFGTVWGVYHALVNIGMSGQGTLDKVAGPVG
                     EALIMTAAGLAVAIPAVLAYNAFVRSNRVVLAQLDSFAHDLYAFLTTGSHVNDVASVV
                     PLKTPALAVRGAT"
     gene            525..944
                     /locus_tag="VHT22_11195"
     CDS             525..944
                     /locus_tag="VHT22_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX3138169.1"
                     /translation="MTFGSFKAGAESAPMAEINMIPLIDVMLVLLVIFIITAPLLTHA
                     VKIDRPKAASHANLTRPDHIALSIDGTGQLYWNGERIDRTHLAPRMAAAAKIEPQPEL
                     HLRADQTTQYQALAEVLAAAGKAGLTRIGFVSDPRNK"
     gene            955..1728
                     /locus_tag="VHT22_11200"
     CDS             955..1728
                     /locus_tag="VHT22_11200"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX3138170.1"
                     /translation="MLQMFVITLREGIEAFLIVAIALAYLRKTGRNALVPAVFWGTGT
                     GVLLSVFLGVELAQFAVQPLWEGILALVAVVLVTSMVVYMLRTAKHLRSHIGARLEAA
                     AQKAGSGAWLGVFAFTVLMITREGMETAFLIDAVALRTGSADIITGALAGTSLAALIA
                     WAWSRFGHRVNLGLFFQVTSIFLLLFALQLLLYSFHELTEAKVLPLDNEYWHEATEAY
                     AEGVYAQIYSLALVLIPAAWLAIAAVKSRSIPRPANGRA"
     gene            1792..1926
                     /locus_tag="VHT22_11205"
     CDS             1792..1926
                     /locus_tag="VHT22_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HEX3138171.1"
                     /translation="MDQPPRRQINSVELFKGQREVLIEHAGEVYSLRHTSKGRLILTK
                     "
BASE COUNT          357 a          656 c          637 g          388 t
ORIGIN      
        1 gcatcccgac gggccgttct cgcatctcac gtggcacgcc atcgtcgccg cgcgccatca
       61 tcagaaccat ggcgccgaca agctcgatgc cgccggcagc acggcagagt tcctgacgcg
      121 cgcgatgcgc cgggtcatcg acgaggaaac cgcgcgcatc gaagcgggtt tgaccgtcct
      181 tgcctcggtc ggcagcacgg cgcccttcgt gggcctcttc ggcacggtct ggggcgtcta
      241 tcacgcgctg gtcaacatcg gcatgagcgg ccagggcacg ctcgacaaag tggccggccc
      301 ggttggcgag gcgctgatca tgaccgccgc cgggctcgcc gttgcgattc ccgcagtgct
      361 cgcctacaac gcattcgtgc gcagcaatcg cgtggtgctc gcgcagctcg actcgttcgc
      421 gcacgatctc tacgctttcc tgaccaccgg ctcgcacgtg aacgatgtcg cttcggtggt
      481 gccgctcaag acgccggcgc tggccgtccg gggagcaacc tgacatgacg ttcggcagct
      541 tcaaggcggg cgccgaatcc gcgccgatgg ctgaaatcaa catgattccg ctgatcgacg
      601 tgatgctggt cctgctggtc attttcataa tcacggcccc gctcttgacg cacgccgtga
      661 agatcgatcg gcccaaggcc gcgagccatg ccaatctgac gagacccgac catatcgcgc
      721 tgtctatcga cggcacgggc caactctact ggaacggcga gcgcatcgac cgcacgcatc
      781 ttgcgccgcg catggcggcg gcggcgaaga tagaaccgca gcccgagctg cacctgcgcg
      841 ccgaccagac cacgcaatac caggcactcg ccgaagtgct cgccgcggcc ggcaaagccg
      901 ggctgacgcg tatcggcttt gtttccgatc cgcgcaacaa gtaagggaac tgccatgctg
      961 caaatgttcg tgatcacgct gcgcgaaggc atcgaagcct tcctgatcgt cgcgatcgcg
     1021 ctcgcgtacc tgcgcaagac cggccgcaac gcgctggtgc cggcggtatt ttgggggacg
     1081 ggtaccggcg tcctgctgtc ggttttcctc ggcgttgagc tggcccagtt cgcagtgcag
     1141 ccattgtggg agggcatact cgcgctggtc gcggtcgtgc tcgtcacctc gatggtggtt
     1201 tacatgctgc gcacggcgaa acatctgcgc agccacatcg gcgcgcggct cgaagccgcg
     1261 gcgcaaaaag cgggcagcgg cgcgtggctc ggcgtcttcg cgttcacggt gctgatgatt
     1321 acgcgcgaag gcatggaaac tgcgtttctg atcgatgccg tcgcgttgcg caccggctcg
     1381 gccgacatca tcacaggcgc cctggccggc acgtccctcg cggcgctgat cgcgtgggca
     1441 tggtcgcgct tcggtcaccg cgtgaatctt ggtttgtttt tccaggtcac ctcgattttc
     1501 ctgctgctct ttgcgctgca gctgctgctg tacagttttc acgagttgac cgaagcgaaa
     1561 gtcctgccgc tcgacaatga atactggcac gaagcgaccg aagcgtacgc cgagggggtg
     1621 tatgcgcaga tttattcgct cgcgctcgtg ctgattccgg ccgcatggct cgcaatcgcc
     1681 gccgttaaaa gccgcagtat cccgcgcccg gcaaacggcc gggcctgatc aactgcagtg
     1741 aaaaccgggg acaaatccgc tgccgtctgc gcgctcgacc gtgcgccggc aatcgatcag
     1801 ccgccgcggc ggcagattaa cagcgttgaa ctattcaaag gtcagcgcga agtgctgatc
     1861 gagcatgcgg gcgaagtcta ttcgctgcgg cacacgagca agggcaggtt gattctgacg
     1921 aagtagcttt tttttgtttt gtagcagcat ttttttgccg agccagcaag tccggcgcac
     1981 cgccgggata gccagccatg cacaaccact gactggagat cctcatggct ggacgtta
//
LOCUS       DATANS010000753         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_207625, whole genome shotgun sequence.
ACCESSION   DATANS010000753 DATANS010000000
VERSION     DATANS010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207625"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VHT22_11210"
     CDS             complement(<1..219)
                     /locus_tag="VHT22_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAA hydrolase family protein"
                     /protein_id="HEX3138172.1"
                     /translation="MTSKQRREILLRASALAAGGAGYAMSGSAAAQARTPFTVAPTFI
                     PIAGSGELFPVRRIYCIGRNYAAHAREMG"
     gene            complement(228..374)
                     /locus_tag="VHT22_11215"
     CDS             complement(228..374)
                     /locus_tag="VHT22_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138173.1"
                     /translation="MSKGKGSGSFRGRDAKTGQPIQIPQVKRPQAPSVPRVMPRTKAS
                     PKGR"
     gene            complement(462..1976)
                     /locus_tag="VHT22_11220"
     CDS             complement(462..1976)
                     /locus_tag="VHT22_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEX3138174.1"
                     /translation="MPEIPRSIAVVGAGLMGRGIAQIAAQAGFDVAIYDVKPDAAAEA
                     RRAIAATLSMLAAKQKLTEDEAARATARIAVVADLGELSRVDLAIEAIVERLDAKQAL
                     FAALEAIVGKRCILATNTSSLSVTAIAAVCANPERVAGLHFFSPVPLMKLVEVIGGLL
                     TDPQVVERLLAFGAALGHTPVRARDTPGFIVNHAGRGYGTEALRILGEGIADVATIDR
                     VLTEGAGFRMGPFALFDLTGLDVSQPVMESIYHQYYEEPRFRPSPLGAQRLAAGVLGR
                     KTGRGFYDYGSGAAPVAPPSVPAAASARRVWVSARDSVARQRVASLVARLGAEVDSDA
                     RPAPDVACLVLPCGCDATSAALDEGLDPTRTVALDVLPDLAKHRTLMTTPVTRPEVRD
                     AVHALFAADGGGVAIIHDSPGFIVQRVLSHIVNVACDIAQQRIAAPADIDLAVRIGLG
                     YPMGPLAWGDALGPARVLAILHELARVYGDPRYRPSLWLSRRARLGVSLLTPEG"
BASE COUNT          384 a          769 c          800 g          362 t
ORIGIN      
        1 gcccatctca cgcgcgtgcg cggcgtaatt gcgaccgatg caataaatgc ggcgtaccgg
       61 aaaaagctca ccgctgccgg caatcggaat gaacgtcggc gcgacagtga acggcgtacg
      121 cgcctgggcc gcagcactgc ccgacatcgc gtagccggcg ccaccggcgg caagcgcgct
      181 ggcacgaagc aggatttccc ggcgctgttt ggatgtcatg gccgcctcta gcgtcctttc
      241 gggctagcct tggtgcgggg catgacccgc ggcaccgacg gcgcctgcgg gcgcttgacc
      301 tgaggaatct ggatcggctg cccggttttg gcgtcgcggc cgcgaaaact gccactgccc
      361 ttgcctttgc tcatcggtcg atctcgcttt ggtgtgagga cacgatcatc atgctactgc
      421 gcaccggcgg ctgcaagcct caccgatcag cgccggtgcg ctcagccctc gggtgtcagc
      481 agcgacaccc cgaggcgggc gcggcgcgac agccacaggc tcggccgata gcgaggatcg
      541 ccgtagacgc gcgcgagctc gtgaaggatg gcgagcacgc gagccggtcc gagggcatcg
      601 ccccacgcca gcggtcccat cggataacca agcccgatcc tgacggcgag atcgatgtcg
      661 gcgggcgccg cgatccgctg ctgcgcgata tcgcaggcta cgttgacgat gtgcgacagc
      721 acgcgctgca cgataaaacc ggggctgtcg tggatgattg cgacgccgcc gccgtcggcg
      781 gcgaacaacg cgtggacggc atcgcgcacc tcgggtcggg tgaccggtgt cgtcatcagc
      841 gtgcgatgct tcgcgaggtc tggaaggacg tcgagcgcga cggtgcgcgt ggggtcgagg
      901 ccttcgtcga gcgcggcgct cgtcgcatcg cacccgcagg gcaggaccag gcaggcgaca
      961 tcgggcgctg gtcgtgcgtc cgaatcgacc tcggcgccca ggcgtgcgac cagcgaggcg
     1021 acgcgctgcc gcgcgacgct atcgcgcgcg ctcacccaga cgcggcgagc cgacgcggca
     1081 gcgggcaccg acggcggagc aaccggtgca gcgccgctac cgtagtcgta aaacccgcgg
     1141 ccggtcttgc gtcccagcac cccggccgcg aggcgttgcg cgcccagcgg cgaaggccgg
     1201 aatcgcggtt cctcgtagta ctggtgatag atcgattcca tcaccggctg cgacacgtcg
     1261 aggccggtca agtcgaaaag ggcgaagggt cccatgcgaa atccggcacc ctcggtcagc
     1321 acgcggtcga tcgtggccac gtcggcgatg ccctcgccga gaatgcgcag cgcttcggta
     1381 ccgtagccgc ggccggcgtg attgacgatg aatcccggtg tgtcgcgggc acggaccggc
     1441 gtgtggccga gcgcggcgcc gaaagccagc aggcgctcga cgacctgcgg gtccgtcaac
     1501 agtccgccga tgacctcgac cagcttcatc agcggtaccg ggctgaaaaa atggaggccg
     1561 gcgactctct ccgggttcgc gcaaacggcg gcgatggcag tgaccgacaa ggacgaggtg
     1621 ttggtcgcaa ggatgcatcg cttgccgacg atggcctcga gcgcagcaaa caacgcctgc
     1681 ttggcatcca ggcgctcgac gatggcttcg atcgccaggt cgacccgcga aagctcgccc
     1741 aggtcggcga cgaccgcaat gcgcgcggtc gcgcgcgcgg cctcgtcctc ggtcagtttt
     1801 tgcttggcgg cgagcatcga caaggtcgcg gcgatcgcgc gccgtgcttc cgccgcggca
     1861 tcgggcttga cgtcgtagat cgctacgtcg aatcccgcct gcgccgcgat ctgtgcaatg
     1921 ccgcgtccca tcaggccggc gccgacgacc gctatcgagc gcgggatttc aggcatcact
     1981 tatcgtcatt cgcttgtcat catgcggcgg ccattatcca atgaacggcg ctgcgaggcg
     2041 aaccatcatc gcgtctccgc ccgtcgggat cggggcgatg gctgggtatg aagtaaaatc
     2101 cgggtcgacc ggctcgccgg ctcgaccgac gcacagcaag gcgaaacgtt tgcaacccgg
     2161 cagcatcgac attcccgcgc tcgtcctgac cgcgaccatc tgggtctact ggttcgctgt
     2221 cggcgcgatg gtcgttcgcg cgcggcggaa gacgcggcac ctcgccggtc tcgttcctga
     2281 gcagccgctg gagcgcagga tgtggctggt tctgg
//
LOCUS       DATANS010000754         2911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_668028, whole genome shotgun sequence.
ACCESSION   DATANS010000754 DATANS010000000
VERSION     DATANS010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668028"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(360..1436)
                     /locus_tag="VHT22_11225"
     CDS             complement(360..1436)
                     /locus_tag="VHT22_11225"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX3138175.1"
                     /translation="MLKPGATIGILGGGQLARMLALAAAPLGLRCHVYSPEKDSCAFD
                     VVHAGTCASYEDETALGRFANAVDVITYEFENVPAKTAAFLAKRKPVLPDPKVLETTQ
                     DRLVEKDFVSALKIATAPYASVHSATQLAEAVKAVGLPAVLKTRQLGYDGKGQAKIAK
                     GGDAIAAWRSVKNAPAILEGFIDFEREASVVAARSRDGAVECFEVTENEHRDHILKFS
                     RVPAKVTPALAREARQVATRIAKAFDYVGVLAVEMFVVRKGRQRTVLVNEIAPRVHNS
                     GHWTIDGATVSQFEQHIRAVAGWPLGKPARLGKRVEMVNLIGDEIDDAARWLTVPGAS
                     VHIYGKGDARAGRKMGHVTRVWPR"
     gene            complement(1440..1925)
                     /gene="purE"
                     /locus_tag="VHT22_11230"
     CDS             complement(1440..1925)
                     /gene="purE"
                     /locus_tag="VHT22_11230"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX3138176.1"
                     /translation="MASKPVAIIMGSQSDWSTMKHAAKTLDALRIGYDARIVSAHRTP
                     KRLYEFASGARKAGFKVIIAGAGGAAHLPGMAASMTPLPVLGVPVNTHALEGKDSLLS
                     ILQMPGGIPVGTLAIGKAGATNAALLAAAVIALSDTKVAKRLDAWRARQTKAVARRPK
                     D"
     gene            2120..2695
                     /locus_tag="VHT22_11235"
     CDS             2120..2695
                     /locus_tag="VHT22_11235"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX3138177.1"
                     /translation="MAEVERLKRELAVARARIGELEARADVDPLLDILNRRGFERELK
                     RALAHAKRYGTPAALMFVDLDSFKAVNDRHGHGTGDALLKAVTREITRHVRASDVVGR
                     LGGDEFGVLLWRVDEAQAVTKARELEGLLARVSVMHGQVHVQVGASVGAALLRADATP
                     AEIITAADRAMYARKDERRGLPSVDYPGSTN"
     gene            complement(2705..>2911)
                     /locus_tag="VHT22_11240"
     CDS             complement(2705..>2911)
                     /locus_tag="VHT22_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX3138178.1"
                     /translation="MTDEEERALREQLARLLQEHRDLDAAISALVTSPGSDLIQIQRL
                     KKRKLILRDKIRRIEDQLTPDIIA"
BASE COUNT          495 a          939 c          954 g          523 t
ORIGIN      
        1 cgcctcatcg tggatgtgcg gagggtggtc tctgcgatag ctcacgccgt catggccggg
       61 cttgtcccgg ccatcccgat tcatgtcgca ctgcttcgcc ctcctaagcg ggatcgccgg
      121 aacaagtccg gcgatgaccg acggagacga catgccacct tcactccggc aatccatgag
      181 gcgtcgcagg cgatcagagc cgcaagaatt ttgcattcgc gcctcatcat ggatgcgcgg
      241 gtcataggcg tgcgaaagca cgccgtcctt cggacggcta tgcccgcgca tgacgctcga
      301 tatcagccga tagctcgcga cgccgggctt gtcccggcca tgacgcgata tggccagctt
      361 cagcgcggcc agacccgggt gacgtgaccc atcttgcgtc ccgcgcgggc gtcgcctttg
      421 ccgtagatgt gcacggaggc cccgggaaca gtcagccagc gcgccgcgtc atcaatttca
      481 tcgccgatca ggttcaccat ttcgacgcgt ttgccgaggc gagcgggttt gccgagcggc
      541 cagccggcaa cggcgcgaat gtgctgttcg aattgtgaca ccgttgcgcc gtcgatcgtc
      601 cagtggccgg aattgtgcac gcgcggagcg atctcgttga ccagaaccgt gcgctggcgg
      661 cccttgcgca ccacgaacat ttccacggcc agtaccccga catagtcgaa agccttggcg
      721 atgcgcgtcg ccacctggcg cgcctcgcgc gcgagggcgg gtgtcacttt cgccggcacc
      781 cgcgagaact tcagaatgtg atcgcggtgc tcgttctcgg ttacttcgaa gcactcgacc
      841 gcgccgtcac gcgaacgtgc agccaccacg gacgcctcgc gctcgaaatc gatgaatcct
      901 tcgagaatgg cgggcgcatt tttcaccgaa cgccaggccg cgatcgcgtc gccgcctttg
      961 gcaatcttgg cctggccctt tccgtcatag ccaagttgcc gcgtcttgag cacggcgggc
     1021 aggccgaccg ccttcacggc ctcggccaac tgcgtcgcgc tatgcaccga cgcatagggc
     1081 gccgtggcga tcttcaatgc actgacgaaa tccttctcga ccagccggtc ctgcgtggtt
     1141 tccagcacct tcggatcggg cagcacaggt ttgcgtttgg cgagaaaggc cgcggtcttc
     1201 gccggcacgt tttcgaactc gtaggtaatg acatcgactg cgttggcgaa acggccgagc
     1261 gcggtctcat cttcgtagga ggcgcaggtg ccggcgtgca cgacgtcgaa agcgcaggaa
     1321 tccttttccg gcgaatacac gtggcagcgc aggccgagcg gtgccgcggc gagcgcgagc
     1381 atacgcgcga gctgtccgcc gccgagaatt ccgatggtgg ctccgggctt cagcaaggtt
     1441 cagtcttttg gccgccgggc gacggctttg gtctggcgcg cgcgccaggc atcgagccgc
     1501 ttggcgactt tcgtgtccga cagggcaatc accgcggcgg cgagcagcgc ggcgttggtt
     1561 gcacccgcct tgccgatcgc cagcgtgccg acgggaatcc cgcccggcat ttgcaggatc
     1621 gagagcagtg aatccttgcc ttccagcgca tgcgtattta ccggcacgcc gaggacgggc
     1681 agcggcgtca tcgatgcagc catgccgggc aggtgcgcgg cacccccggc gccggcgatg
     1741 atgaccttga aaccggcctt gcgcgcgccg cttgcgaact cataaaggcg cttgggtgtg
     1801 cgatgggccg agacgatgcg ggcgtcgtaa ccgattcgca gggcgtcaag ggtttttgcc
     1861 gcgtgcttca tcgtggacca gtcggactgg ctgcccatga tgatcgcgac cggttttgac
     1921 gccattttgc tccccgcact ccgcattttc ctgcggaagg aagcgccgga ttataggttg
     1981 aggctccccg ctggcaaggc gaaaggagta ttctctcgcg caaggttaag attcgcgagc
     2041 tcacagacga gggacagaca tgggtttgcc gcaagccaag ccaagcgtac gcgaggagtt
     2101 tccacccgag gcgtcgctga tggccgaggt cgagcgcctc aagcgcgaac tggcagtcgc
     2161 gcgcgcccgc atcggtgagc ttgaggcgcg ggccgacgtt gacccactgc tcgatatcct
     2221 caatcgccgc gggttcgagc gcgaactcaa acgcgcgctc gcgcatgcca agcgttacgg
     2281 caccccggcc gcgctgatgt tcgtggacct cgacagtttc aaggccgtca acgaccgcca
     2341 cggccacggg accggcgacg ccttgctcaa ggccgtcacg cgcgagatca cccgccatgt
     2401 ccgcgcctcc gacgtcgtgg gacggctggg cggcgacgaa ttcggcgtgt tgctctggcg
     2461 cgtcgacgag gcgcaggccg tgacgaaagc gcgcgagctg gaaggactgc tcgcccgggt
     2521 cagcgtgatg cacgggcagg tgcacgtgca ggtcggcgcc agcgtcggtg ccgcgctgct
     2581 ccgcgccgat gccacacccg cggagatcat cacggccgcg gaccgcgcaa tgtatgcgcg
     2641 aaaggacgaa agacgcggcc tgccgtccgt cgactatccg ggcagcacga actgaggaaa
     2701 aaacctaggc gatgatgtcc ggcgtgagct ggtcttcgat gcggcggatc ttgtcgcgca
     2761 ggatgagctt ccgcttcttc agtcgctgta tctgaatgag gtcggaaccg ggcgaggtga
     2821 ccaaggccga tatcgccgcg tcgaggtcgc ggtgttcctg cagcaggcgc gccagctgct
     2881 cccgcaacgc gcgttcttct tcgtcagtca t
//
LOCUS       DATANS010000755         3076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_459681, whole genome shotgun sequence.
ACCESSION   DATANS010000755 DATANS010000000
VERSION     DATANS010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459681"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VHT22_11245"
     CDS             complement(<1..443)
                     /locus_tag="VHT22_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014984926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3138179.1"
                     /translation="MTTFRTLATVRHHGMELTDYEFGVPLDHDQPSGETLSIFARAVR
                     NSEAASAKRPWLVFLQGGPGFPGPRPLDKSGWLKRALDDYQVLLLDSRGNGRSSVVLP
                     QTLARRGNARAQADYLMHFRADSIVRDAEAIRRQLVGEDEQWGVLG"
     gene            complement(440..1816)
                     /locus_tag="VHT22_11250"
     CDS             complement(440..1816)
                     /locus_tag="VHT22_11250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3138180.1"
                     /translation="MTAPESPLRPFPANQRSCSTLVVGAGIVGVATALRLQREGRDVW
                     LIDRERVAAQASRGNAGALAFSDILPLASAGILRQAPRWLLDPLGPLTIRPSYLPRLV
                     PWLLRFWHASRRARVRASTAAQAALMTLSAAETPLMLEAAGASHMLRSEGVLHLYESE
                     AELQASQAGWQARAEHGIAFTHLHGAQSIAPFQPGLSAAIVAATFVPAWSTVSDPLQL
                     TLALAREFERLGGRIGRADVIALSSGTDGVSVLLREGPPIVAGRVVVAAGAWSHRLAR
                     TLGERIPLETERGYNTTLPSGAFDLKRQLTFGGHGFVVTRLACGIRIGGAVEFAGLDA
                     APNFARSAALLAKAKRFMPGLRTEGGSEWMGFRPSLPDTLPAIGPSESDDRVVYAFGH
                     GHLGLTQAAATARLVADIVAARSLPIDLVPYRPQRFARGGPGRAARKIIPPTPTENPD
                     RPTQIENS"
     gene            complement(1877..2851)
                     /locus_tag="VHT22_11255"
     CDS             complement(1877..2851)
                     /locus_tag="VHT22_11255"
                     /EC_number="5.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyproline epimerase"
                     /protein_id="HEX3138181.1"
                     /translation="MNFDPSVPVRVIDSHTGGEPTRVVVSGGPDLGSGDMAQRRQRFR
                     DKYDAWRRAIVNEPRGSDVLVGAFLCAPVDATNACGVIYFNNVGYIGMCGHGTIGLVA
                     TLAYLGRLKAGDHRIETPVGIVTTTLHDDGSVTVANVPAYRHATQLRVHVPGHGAVHG
                     DVAWGGNWFFLCSDHGLRLEARQVAELSEFASRVRDALRQHGITGAAGAEIDHVELIG
                     PPADPRNDARNFVLCPGGAYDRSPCGTGTSAKLACLAADGKLAPGAFWRQESVIGSVF
                     TAHYQRPAQALPDSPANAVLPLIHGRAHVTLDANLVFDRADVFTWGLV"
     gene            2897..>3076
                     /locus_tag="VHT22_11260"
     CDS             2897..>3076
                     /locus_tag="VHT22_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138182.1"
                     /translation="MKRPAVTKPESVRDPTRHAPSLHALSIAIVFALSLLAAPAFPAD
                     PAKTLRVVLTSAEQSF"
BASE COUNT          516 a         1032 c         1049 g          479 t
ORIGIN      
        1 ccgagcacgc cccactgctc gtcctcgccg accagctggc gacggatagc ctcggcatcg
       61 cggacgatgc tgtcggcgcg aaaatgcatc aggtaatcgg cctgcgcgcg ggcgttgccg
      121 cgccgggcca gcgtctgggg caacacgacg ctgctgcggc cattgccgcg cgaatcgagc
      181 agcagtacct ggtaatcgtc gagagcccgc ttgagccagc ccgatttgtc gagcggtcgc
      241 ggtccgggga atccgggtcc tccctgcagg aacaccagcc agggccgctt cgccgacgcg
      301 gcttcgctgt tcctcacggc ccgcgcgaaa atcgacaagg tctcgcccga cggctggtcg
      361 tggtcgagcg ggacgccgaa ttcgtagtcg gtcagttcca tgccgtgatg gcggacggtg
      421 gcgagcgttc ggaatgtcgt catgagtttt cgatttgggt tggccgatcc ggattctcag
      481 tcggcgtggg cgggatgatt ttccgcgctg ctcgcccggg tccgccgcga gcgaaacgct
      541 gcggacgata gggtaccaag tcgatcggca acgaacgggc cgccacgatg tcggcgacca
      601 gtcgcgccgt cgcggcggcc tgcgtgagcc cgaggtggcc gtggccgaac gcatagacga
      661 cgcgatcatc gctctcggac gggccgatcg ccggcagcgt gtcgggcagc gacggacgaa
      721 aacccatcca ttcgctgccg ccttcggtgc gcagtccggg cataaaacgc ttcgctttcg
      781 caagcagtgc ggccgagcgc gcgaagttgg gcgctgcatc gagcccggcg aactccacgg
      841 cgccgccgat gcgaatgccg cacgccagcc gcgtcacgac aaaaccgtgt ccgccgaagg
      901 tgagctggcg cttcaagtcg aaggctcccg acggcagcgt ggtgttgtag ccgcgttcgg
      961 tctcgagcgg aatgcgctca ccgagcgtgc gcgcgaggcg gtgcgaccag gcaccggcgg
     1021 cgacgaccac ccggcccgcg acgataggcg gcccttcgcg caaaagcacg ctgacgccgt
     1081 cggtcccgga ggagagcgcg atcacatcgg ctcgtccgat gcggccgccg agccgctcga
     1141 actcgcgcgc cagcgccagc gtcaattgca gcggatcgct gacggtgctc caggcgggca
     1201 cgaaagtcgc cgccacgata gccgcgctca ggcccggctg gaatggggcg atcgactgcg
     1261 caccgtgcag gtgagtgaag gcgatgccat gctcggcgcg ggcctgccat ccggcttgcg
     1321 atgcctgcag ctcggcttcg ctctcgtaaa gatgcaggac gccctcgctg cgcagcatgt
     1381 ggctggcacc ggccgcctcg agcatcaaag gcgtttcggc cgcggacagc gtcatcagcg
     1441 ccgcctgcgc ggcggtgctc gcgcgcacgc gggcgcgccg actggcatgc cagaaacgca
     1501 acagccatgg gacgaggcgt ggaaggtaac tcggtcggat cgtcagcggg cccagcggat
     1561 ccagcagcca gcggggtgct tggcgcagga tccccgccga ggcgagcggc aggatgtccg
     1621 agaacgccag cgcgccggcg ttgccgcggc tggcctgcgc cgccacacgc tcccgatcga
     1681 tcagccatac gtcgcgtcct tcgcgttgca gtcgcagcgc agtcgccacg ccgacgattc
     1741 cggcgccgac cactagggtg ctacatgatc tctggttcgc cggaaacggt cgcaacggcg
     1801 attcgggcgc agtcatccga aataacgtag tgatcgattt gtgcacgcga gggcgagcgc
     1861 gctgctcatt gcggattcag accagccccc atgtgaagac gtccgcgcgg tcgaagacga
     1921 gattcgcatc gagggtgacg tgagcgcggc cgtggatcag cggcagcacc gcattcgccg
     1981 gcgaatccgg aagcgcttgt gccggccgtt gatagtgtgc ggtgaacacg ctgccgatca
     2041 cgctttcctg gcgccagaaa gcgcccggtg ccagcttgcc atcggcggcc aggcatgcga
     2101 gcttggcgct cgttcccgtg ccgcagggcg agcgatcgta ggccccgccc gggcagagca
     2161 cgaagttgcg ggcgtcgttg cgtggatcgg ccggcggacc gatcagctcg acatgatcga
     2221 tctcggcccc ggcagctccc gtgatgccgt gctgccgaag cgcgtcgcgc acgcgcgaag
     2281 cgaactcgga aagctcggcg acttggcgcg cttcgaggcg caagccgtgg tcgctgcaca
     2341 gaaagaacca gttgccgccc cacgccacgt cgccatgcac ggccccatga cccggaacat
     2401 gcacccgcag ctgcgtcgcg tggcggtagg caggtacgtt ggcgacggtc acgcttccgt
     2461 cgtcatgcaa ggtcgtggtg acgatgccga ccggagtttc gatccggtga tcgcccgcct
     2521 tcaaccggcc gagataggcc agcgtcgcga ccagccctat cgtgccatgc ccgcacatgc
     2581 cgatgtagcc gacgttgttg aagtagatca ccccgcaggc attggtcgcg tcgacgggcg
     2641 cacataagaa cgcgcccacc agcacgtccg aaccgcgggg ctcgttgacg atggccctgc
     2701 gccacgcatc gtacttgtcg cgaaagcgct gccggcgctg tgccatgtcg ccgctgccca
     2761 ggtctggtcc cccgctgacg accacacggg tgggctcgcc gccggtgtgc gagtcgatga
     2821 cgcggacggg tacggacgga tcgaagttca ctcttgggaa cgggcggcgg cgcagaagtc
     2881 gattatagtc gagaccgtga aacgccccgc cgtcacaaag ccggagtcgg tgcgcgaccc
     2941 aacgcgccac gcacccagtc ttcatgcatt gtcaatcgcg atcgtcttcg cactgagttt
     3001 gcttgctgct ccggctttcc ccgccgaccc ggcgaaaacg ctgcgcgtcg tcctcaccag
     3061 cgcggaacag agtttc
//
LOCUS       DATANS010000756         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_374103, whole genome shotgun sequence.
ACCESSION   DATANS010000756 DATANS010000000
VERSION     DATANS010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374103"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /gene="ggt"
                     /locus_tag="VHT22_11265"
     CDS             complement(<1..1537)
                     /gene="ggt"
                     /locus_tag="VHT22_11265"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX3138183.1"
                     /translation="MSNHPAGAVLRALFNIEVVMKLRARLLPWRHVAIVLLACITVSA
                     HAAEGRARAAIATAHPLATEAGYRILEQGGNAFDAAVTIAAVLAVVEPYSAGLGGGGF
                     WLLHRASDKKQVMIDARETAPAGVALSMYVDADGVPVPGATLRGGKAAGIPGVPAGMV
                     HLAQRYGKLRLRQLLAPAIALARNGFNLDSRFARIAKVREPLLRDGVNTARIFLDNGA
                     APPQGYLLRQPELAATLERLAEQGRDGFYRGLVAQALVSGVNAAGGVWQAADLADYKI
                     IERPPVRFSYRGATVVSAALPSSGGVTLAQTLNILEGYKIADARDPAEAHIVIEALRR
                     GFQDRLRYLGDTDFVQVPVAKLVDKAYAHSRAASINPNIATPLDALTITPLAAVTESY
                     NTTHYSVIDVDGNRVGATLSINSWFGAGVIAGDTGVLLNNEIDDFSLGAGLPNLYRLL
                     GGKANSIEPHKRPLSSMSPTFVEDSKGVLVIGAPGGSRIISMVLFGILDYVNQDSVDL
                     LRIV"
     gene            complement(1669..>2190)
                     /locus_tag="VHT22_11270"
     CDS             complement(1669..>2190)
                     /locus_tag="VHT22_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009108060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3138184.1"
                     /translation="VTREYERGTMENLLATPVRPIEVMIGKIVPYIIVGYIQVTVILL
                     AAKLLFAVPMLGSLALLSVVLILFIAANLAIGYTFSTLAKNQMQAMQMTFFFFLPSML
                     LSGFMFPFRGMPQWAQWLGEALPLTHFLRIVRGILLKGNGFSDIAPNMWPLALFLLIA
                     GTIALTRYRETLD"
BASE COUNT          432 a          721 c          686 g          351 t
ORIGIN      
        1 tcacgatgcg cagcaaatcg acgctgtcct ggttcacgta atcgaggata ccgaacagca
       61 ccatgctgat aatgcgcgag ccgcccggcg caccgatgac cagcacgcct ttgctgtcct
      121 ccacgaacgt gggactcatg ctcgacaaag gccgcttgtg cggctcgatc gaattggctt
      181 tgccgccgag caggcgataa agattgggca agccggcgcc cagcgagaag tcgtctattt
      241 cgttgttcag cagcacgccg gtatcgccgg caatcacgcc ggcgccgaac cacgagttga
      301 tgctcaaggt ggcgcccacg cggttgccgt cgacatcgat caccgagtaa tgcgtagtgt
      361 tatagctttc ggtgacggcc gcgagcggcg taatggtcaa cgcgtcgagc ggcgtagcaa
      421 tgttcggatt gatcgatgcg gcgcggctgt gcgcgtacgc tttgtcgacc agtttcgcga
      481 ccggcacctg cacgaagtcg gtgtcgccca gataacgcag ccggtcctgg aagccgcggc
      541 gcagcgcttc gatcacgata tgcgcttccg cggggtcgcg cgcatcggcg atcttgtagc
      601 cttcgaggat gttgagcgtc tgggcgagtg tcacgccacc cgacgacggc agcgccgccg
      661 aaacgaccgt cgcgccgcgg tagctgaaac gcacgggcgg ccgctcgatt atcttgtaat
      721 cggccagatc cgccgcctgc cacacgccgc cggcggcatt gacgccgctc acgagcgcct
      781 gcgcaaccag cccgcggtaa aaaccatcgc gcccctgctc ggccaggcgc tcgagcgtcg
      841 ccgcgagttc cggctggcgc aacagatagc cttgcggtgg cgccgcgccg ttgtcgagaa
      901 agatccgcgc cgtgttcacg ccatcgcgca gcagcggctc gcgcaccttg gcgatacgcg
      961 caaaccgcga atcgagattg aaaccgttgc gcgcgagcgc gatggccggc gcgagcaact
     1021 gccgcaggcg cagcttgcca tagcgctggg cgagatgcac catgccggcc ggcacgcccg
     1081 gaatcccagc cgccttgccg ccgcgcaggg tcgcccccgg taccggtacc ccatctgcat
     1141 cgacgtacat ggatagcgcg actccggcag gcgcggtttc gcgcgcgtcg atcatgacct
     1201 gcttcttatc gcttgcccgg tgcagcagcc agaatccgcc gccgcccagg cccgccgaat
     1261 agggctcgac gacggccagc acggcggcga tcgtcacggc cgcgtcgaac gcattgccgc
     1321 cctgctccag aatccggtag ccggcttcag tggccagcgg atgagccgtg gcaattgccg
     1381 cgcgcgcccg cccttccgct gcatgcgcgg acacggtgat gcacgcgagc aaaactatgg
     1441 cgacgtgccg ccacggcagg agccgcgccc ttagcttcat caccacttca atgttgaaca
     1501 aagcccgcag caccgcgccg gccggatggt tagacaatgt tgtcctcata aaaacgttct
     1561 gcgggaagaa cgatagccgc cgcatccacc gggacgctcg gccgctgatt caccggcgca
     1621 gtataccagc gcgcgtcgcc gcatcggcag ttcgcgcgcc gcgagccctc aatccagtgt
     1681 ctcgcgataa cgggtcagcg cgatggtgcc ggcgatcagc aaaaacagag caagcggcca
     1741 catgtttggc gcgatgtcgg agaacccgtt gcccttgagc aggatgccgc gcacgatgcg
     1801 cagaaaatgc gtgagcggca gcgcttcgcc aagccattgc gcccattgcg gcatgccgcg
     1861 aaacgggaac ataaagcccg acagcagcat cgacggcaga aaaaagaaaa aagtcatctg
     1921 catcgcctgc atctgattct tggccagcgt cgagaacgta tagccgatcg ctaaattggc
     1981 ggcgatgaac agaatcagga cgaccgacag caaggcgagg ctgccgagca tgggcaccgc
     2041 aaacaggagc ttggcggcga gcaggatcac cgtgacctgg atatagccga caatgatgta
     2101 cggcacgatc ttgccaatca tcacctcgat cggccgcacc ggggtggcga gcaggttctc
     2161 catggtgccg cgctcgtact cgcgcgtgac
//
LOCUS       DATANS010000757         1809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_459898, whole genome shotgun sequence.
ACCESSION   DATANS010000757 DATANS010000000
VERSION     DATANS010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459898"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1173
                     /locus_tag="VHT22_11275"
     CDS             217..1173
                     /locus_tag="VHT22_11275"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX3138185.1"
                     /translation="MKLTHAAGMAALLTAAVAGAAETQFPTRPIRLLVPQAAGGSNDI
                     MARNVANHLTQTFGKQVVVDNRPGAEGIIGTEIVARSAPDGYTLLLASAAFTMNPAVR
                     KLPYDSLTAFDWVAKLGSGPTVLTVGPLLPVGTVKEILAIGKEKPGVITMASAGGFQH
                     FGTALFRSLSGVDFTILLYKGGFPAMIDVMGGQSHMTVGSIVQSIPHIRSGKLKPIAT
                     GGATRAATLPDLPTIAEAGVPGYDASNWWAVAAPAGTPAPTISKLSAAITAYLNMPET
                     QKRFTTEGAEVDIRTPAEMRKMIPLDMAKWARVAKEAGMRAE"
     gene            1221..>1809
                     /locus_tag="VHT22_11280"
     CDS             1221..>1809
                     /locus_tag="VHT22_11280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3138186.1"
                     /translation="MELALKGRIAFVTGASRGIGRAIALALAAEGVHLGLFGRDTGRC
                     DALAADLKKKYLELRTCVVPLDLEGGTAKIKPAIESAIKALGGVDILINCAGGAYRGR
                     LEQIPDDAWERYFAVKPLGLIRMTREALPYLKKSPQGRVVNISGTRGREPGGPSVMSG
                     PINLGTLSITKALANELGPLGITVNAICPGSTDTGR"
BASE COUNT          344 a          568 c          602 g          295 t
ORIGIN      
        1 cgtatggcgc gccggtttct tcgagcgcga cgtgcgaagc ggtcgagcag gcgccggggc
       61 aaaaataaag cgtgagcatg gatggaactc ctgacgggtg gctattggga aaagcggaat
      121 actgcgaatg ccgctggatt ataaaccgtc gcggcttgcc aacgacgcag tgcgcgctat
      181 catggccgca taaacggacc accggaggag acacgtatga agttgaccca cgcagcgggc
      241 atggccgcgc tgctgaccgc tgccgttgcg ggggccgcgg aaacccagtt tccgacccgg
      301 ccgatccgcc tgctcgtgcc gcaggcggcc ggcggcagca acgacatcat ggcgcgcaac
      361 gtcgcgaacc atctgacgca gactttcggc aagcaggtcg tggtcgacaa ccggccgggc
      421 gcggaaggca tcataggcac agagattgtc gcgcgctccg cgcccgacgg ctatacgctg
      481 ctgctcgcat cggctgcatt cacgatgaat ccggcagtgc gcaaactgcc gtatgactcg
      541 ctgaccgcgt tcgactgggt cgccaagttg ggttccggcc cgaccgtgct gaccgtcggg
      601 ccgctgttgc cggtcggcac ggtcaaggag attctcgcca tcgggaaaga gaagccgggt
      661 gtcatcacca tggccagcgc gggcggcttc cagcatttcg gcaccgcgct gtttcgcagc
      721 ctctccggcg tcgatttcac aatcctgctg tacaagggcg gctttcccgc gatgatcgac
      781 gtgatgggcg ggcagtcgca catgaccgtg ggctcgatcg tgcagtcgat cccgcacatc
      841 cgctcgggca agctgaagcc gatcgcgacc ggcggcgcaa cgcgcgcggc gacgctgccg
      901 gatttgccca ccatcgccga ggccggcgtg ccgggttacg acgcgagcaa ctggtgggcg
      961 gtggcggcgc cggctggcac gccagctccg acaatttcga agctcagcgc ggcgatcacg
     1021 gcttatctca atatgccgga aacgcaaaag cgcttcacga ccgagggcgc tgaagtcgac
     1081 atccggacgc cggcggagat gcgcaaaatg attccgctcg acatggcaaa atgggcgcga
     1141 gtcgccaaag aagcgggcat gcgcgccgag tagcctcgtg cggcgcgccg ccgcatcgtc
     1201 atcaatcact cgggaacacc atggaacttg ctctgaaagg acggattgcg tttgtcaccg
     1261 gcgcgagccg gggcatcggc cgcgcaatcg cattggctct tgccgccgaa ggcgtgcatc
     1321 tggggttgtt cgggcgcgac acgggccgtt gcgatgcgct cgctgccgat ctcaagaaaa
     1381 agtatctgga gcttcgcacc tgcgtcgtgc cgctcgacct cgaaggcggc accgcgaaaa
     1441 tcaagccggc gatcgagagt gcaatcaaag cgctcggggg cgtggacatc ctcatcaact
     1501 gcgcaggcgg cgcttaccgc gggcggctcg agcagatacc cgatgacgcg tgggagcgtt
     1561 atttcgccgt gaaacctctc ggcttgatcc gcatgacgcg cgaagcgctg ccctatctga
     1621 aaaaatcacc gcagggccgc gtggtcaata tctcgggcac gcgcggccgc gagcccggcg
     1681 gtccgtcggt gatgtcgggg ccaatcaatc tcggtacgct gtcgatcact aaggcgctgg
     1741 cgaacgagct cggaccgctc ggcatcacgg tgaacgcgat ctgcccgggc tcgacagaca
     1801 ccggacgct
//
LOCUS       DATANS010000758         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_459964, whole genome shotgun sequence.
ACCESSION   DATANS010000758 DATANS010000000
VERSION     DATANS010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459964"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VHT22_11285"
     CDS             <1..232
                     /locus_tag="VHT22_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HEX3138187.1"
                     /translation="EFYRDFEWMGLQRHLKVLGIFARLNYRDGKSSYLDDTPRFIGYA
                     RAVAARYAALRPLLTLLDQIENRPAPRTGYTF"
     gene            300..986
                     /locus_tag="VHT22_11290"
     CDS             300..986
                     /locus_tag="VHT22_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX3138188.1"
                     /translation="MKAMILAAGRGERMRPLTDKLPKALLAVGGKPLITWHVEKLVAA
                     GFADIVVNHAHLGALIEAALGNGRQFGANIIYSAETQALETAGGIANALHLLGDAPFA
                     VVNADVFSDFDYRGLAGSVARLAAPDVLAHLILVANPEHHAGGDFALRAGRVALDGER
                     HTFSGIAAYRPTLFQALVPGSSAKLAPLLREQIAARRVSGELFAGRWRDIGTPARLAS
                     LERELATRWQ"
     gene            1016..>2200
                     /locus_tag="VHT22_11295"
     CDS             1016..>2200
                     /locus_tag="VHT22_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004547630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEX3138189.1"
                     /translation="MAQPPTAPPVVSVHAERRARLAQRMQNGIAVIPTAPERTRNRDS
                     HYPYRYDSYFYYLTGFIEPAAVLVVIAGAAPRSILFCRDKDKEREIWDGFRYGPEAAC
                     GAFGMDECHSIAELDARMPALLADQPQLCCHLGSDDAWDARVLGWLNQVRAEVRSGVA
                     APAAITDVRTLLDAMRLIKDGHELATMRGAARISTAAHQRAMRATAPGRAEFEIEAEL
                     LHEFRRGGAQAPAYTSIVAGGANACVLHYVQNDAVLKAGDLLLIDAGCELDGYASDIT
                     RTFPVSGQFSAPQRDVYELVLAAQAAALATVAPGTAWNAPHEAAVRVLAQGFIDFGLC
                     QGSVDQVIENGDYRKYYMHRTGHWLGLDVHDAGEYKDQGAWRKLEPGMVLTVEPGCYI
                     QAG"
BASE COUNT          377 a          726 c          717 g          380 t
ORIGIN      
        1 cgaattctat cgggacttcg agtggatggg cctgcagcgc catcttaagg tgctcggcat
       61 tttcgcgcgc ttgaactacc gcgacggcaa aagcagctat ctcgacgaca cgccgcgatt
      121 catcggttat gcgcgcgcgg tggcagcacg ctacgcggcg ctgcgcccgt tgctgacttt
      181 gctcgaccaa atcgaaaacc ggccggcccc gcgcaccggc tacacatttt gaccgcattg
      241 cgtgccaacg cagtgccgag cgtcaaagtt caacgctgcg ctgaatgtcc gggccgcgca
      301 tgaaagccat gatcctcgcg gccggccgcg gcgaacgcat gcggccgctg accgataagc
      361 tgcccaaggc gctgctcgcc gtcggcggca agccgctgat tacgtggcac gtcgaaaagc
      421 tcgtcgcggc cggtttcgcc gatatcgtcg tcaaccacgc gcatctgggc gcgctgatcg
      481 aagccgcgct tggcaacggc cggcaatttg gcgccaacat catctattcg gcggaaactc
      541 aagcactcga aaccgcaggc ggcatcgcca acgcactgca tctgctgggc gacgcaccgt
      601 tcgccgtggt caatgccgac gtgttcagcg attttgatta tcgaggtctg gccggcagcg
      661 tggcccgcct cgcagcaccc gacgtgctcg cccatttgat tctggtcgcc aatcccgagc
      721 accatgcggg cggcgacttt gcgctgcgcg ccggccgcgt cgcgctcgac ggtgagcgcc
      781 atacatttag cggtatcgct gcgtaccggc cgacgctgtt tcaggcgcta gtcccgggtt
      841 ccagcgccaa actcgcaccg ctgctacgtg agcagatcgc cgcgcggcgc gtgagcggag
      901 aactgtttgc aggacgctgg cgcgacatcg gcacgcccgc gcggttggcg tcgctcgagc
      961 gcgaactcgc gacgcgttgg caatagcggc cgggtgcgcg gcgtataatt tgcacatggc
     1021 acaacccccc accgccccgc cggttgtcag cgtccatgcc gagcggcgcg cgcggctcgc
     1081 ccaacgcatg caaaacggca ttgcggtcat tccgaccgca cccgagcgca cgcgcaaccg
     1141 cgattcgcat tatccgtacc gctacgacag ctatttttat tacctgaccg gtttcatcga
     1201 gcccgccgcc gtgctggtgg tgatcgccgg cgctgcgccg cgcagtattc tgttctgccg
     1261 cgataaagat aaagagcgcg aaatctggga tggcttccgt tatggtcctg aggccgcttg
     1321 cggcgcgttc gggatggacg agtgccacag catcgccgaa ctcgatgcgc gcatgcccgc
     1381 gctgctcgcc gatcagccgc agctctgctg tcatttgggc agcgatgacg catgggacgc
     1441 gcgggtcttg ggctggttga accaggtgcg cgctgaggtg cgcagcggcg tcgccgcgcc
     1501 ggccgcgatt accgacgtgc gcacgctgct cgacgcaatg cgccttatca aggatggcca
     1561 cgagctcgcg acgatgcgcg gcgccgcccg gatttcgact gccgcgcatc aacgcgcgat
     1621 gcgggcgacc gcgccgggcc gcgcggaatt tgaaatcgaa gccgagttgc tgcacgaatt
     1681 ccggcgcggc ggcgcgcaag cacccgctta cacgtcgata gtggccggcg gcgcgaacgc
     1741 gtgcgtgctg cattacgtgc agaacgacgc ggtgctgaag gctggcgacc tgctgctgat
     1801 cgatgccggt tgcgagctcg atggttacgc gtccgacatc acgcgcacgt ttcccgtgag
     1861 cggccaattc agcgccccgc agcgcgatgt gtacgagctg gtgctcgccg cccaggcggc
     1921 ggcgttggcg acggtcgcgc cgggcaccgc gtggaacgcg ccgcatgaag cggccgtgcg
     1981 ggtgctggcc cagggcttca tcgactttgg cctgtgtcag ggcagtgtcg accaggtgat
     2041 cgaaaacggc gactaccgca aatactatat gcaccgcacc ggccactggc tggggctcga
     2101 cgtgcacgac gcgggcgaat acaaagatca aggcgcctgg cgcaagctcg agcccggaat
     2161 ggtgctcacg gtggaacccg gctgctatat acaagccggc
//
LOCUS       DATANS010000759         3635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_79003, whole genome shotgun sequence.
ACCESSION   DATANS010000759 DATANS010000000
VERSION     DATANS010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79003"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VHT22_11300"
     CDS             complement(<1..621)
                     /locus_tag="VHT22_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HEX3138190.1"
                     /translation="MNIILVSGATARARTLSLDWRHWTAGGFGLLALFLSFTLLFNYV
                     TLRYAASINHPLLQAILLADQREEAQKTQEVVQGHLNAMAVKLGDLQARMLRLDGLGE
                     RLAKLAGLKPQELPGVLQPGVTPGRGGPAPTLQRNLSLDEFNALLAQLAQQVDQRSDQ
                     LGVLEALLVSDSANKKFLPTLKPIEDAWFSSNFGWRLDPFTGQQSFH"
     gene            complement(1120..1296)
                     /locus_tag="VHT22_11305"
     CDS             complement(1120..1296)
                     /locus_tag="VHT22_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138191.1"
                     /translation="MRMLLFLALAAIAGAGVAYLFKRDRRYLRFIGRVIQYTILLLLG
                     ILVFYAFERLVVVV"
     gene            complement(1392..2309)
                     /gene="lpxC"
                     /locus_tag="VHT22_11310"
     CDS             complement(1392..2309)
                     /gene="lpxC"
                     /locus_tag="VHT22_11310"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX3138192.1"
                     /translation="MMKQRTLKSKIKTTGVGLHTGARVELTLRPAHPETGIVFHRVDL
                     PGSAAIPGEARNVGDTRLSSSLSKDGASVSTVEHLMSALAGLGIDNLHVDVAGPELPI
                     MDGSAAPFVFLLQSAGIVEQEAAKRYLRIKMPVEARDGDKWARFEPHHGFVLDFTIDF
                     PHPVFGSENRHVIIDFAENSYIKEVSRARTFGFMQDVEAMRSAGLALGGSLQNAIVLD
                     ETRVLNSEGLRYDNEFAKHKVLDAIGDLYLLGHPMIGRYTAFKSGHALNNALSRALLD
                     RPEAWEIVTFDAQSDVPRAFQGWLLAQPA"
     gene            complement(2479..>3635)
                     /gene="ftsZ"
                     /locus_tag="VHT22_11315"
     CDS             complement(2479..>3635)
                     /gene="ftsZ"
                     /locus_tag="VHT22_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX3138193.1"
                     /translation="QNPQEAVIKVIGVGGCGGNAIEHMIERQLQGVEFIAANTDAQAL
                     KRSTARIQLQLGSNLTRGLGAGARPEIGRDAAMEDKDRIGEMIDGADMLFITAGMGGG
                     TGTGAAPIVAQVAKDRGILTVAVVTRPFLFEGKRQRVAEAGIEELKKTVDSLIIIPND
                     KLMQVLGEDVSVLDAYAAANDVLHSAVAGIAEVINNPGLVNVDFADVRTVMSEVGMAM
                     MGSAKVGGPDRARLAAERAVASPLLEEINLAGARGVLVNITASAGLKMKEYHEVMNTI
                     KSFTADDAMVIVGTVIDEAMKDELRVTMIATGLGGATVRNRQPRLEVVEHVVDRTGTD
                     NVGMSIETVDYDSLDKTPAVIRRGRTPSPASGASAFDASDIPAFLRKQAD"
BASE COUNT          625 a         1216 c         1159 g          635 t
ORIGIN      
        1 gtggaaactc tgctgtccgg tgaacggatc gaggcgccag ccgaagttgg acgagaacca
       61 ggcatcctcg atcggcttca aggtcggcag aaacttcttg ttggccgaat cgctcaccag
      121 cagcgcttcc agcacgccga gctggtcgga gcgctggtcg acctgctgcg cgagttgcgc
      181 cagcagcgcg ttgaactcat cgagcgacag attgcgctgc aaggtcggcg ctgggccgcc
      241 gcgtcccggt gtcacccccg gctgcagcac gcccggcaat tcctgcggct taagccccgc
      301 gagcttggcc agccgctcgc ccagtccgtc gaggcgcagc atcctcgcct gcaggtcgcc
      361 cagcttgacc gccatcgcat tcaggtggcc ctgcacgacc tcctgcgttt tctgcgcctc
      421 ttcccgctgg tcggccagca ggatcgcctg caacagcgga tgattgatgg acgccgcgta
      481 gcgcagcgtc acatagttga acaacagcgt gaatgagagg aaaagcgcga gaagaccgaa
      541 tccgccggcc gtccagtgtc tccagtcgag gctcagcgtg cgggcccgcg ccgtggcgcc
      601 ggaaaccagg ataatgttca aacggaatct ccctgcgatc gtcggcgcgc gaggcggcgt
      661 ccggcttctg gcaaatatgc taacgtcatg cggctcctag ggcaagtact ccaaaacgaa
      721 gccgcgctgg ccgatctcca gcgccgccgg gagcgtgagc tccgcttgca actacgattg
      781 cgcggggcgt tgccgcccgc gctggccacc cacgtcagta tcgcggatgc ccaatcccag
      841 gagctccaac ttgtggctgc cagcggtgcc gcggcggcat tgctgcggca acgcgcgccc
      901 gatgtcctgc acaaacttgc cggggagggg tgggaattta ctggaataag ggtgcgcgtg
      961 caagcccgcc cggcggtgac cgaaccggcg aaaaccccta agaaacaatt ggatgaggcg
     1021 gcggcgacca gcctcgaggc actggcctcc cgcttgggcg acgagcctct ggcccaagcg
     1081 ctcaggcgcc tggcgcgcca ttcgccccgg cgcgcgaagt cagacgacga cgaccagccg
     1141 ctcgaacgcg taaaagacca ggatccccag caatagcagg atggtgtatt ggatgacgcg
     1201 gccgatgaag cgcagatagc gccggtcgcg cttgaacagg taggctacgc cggcgccggc
     1261 aatcgccgcc agcgccagaa acagcagcat ccgcaccaga accaagggat tccttaaaga
     1321 ggagcgcgcc ggatcatccc gcggggcgcg gcgtcccggc tccggcctcg accggcttga
     1381 cggttatcga gctaggccgg ctgcgccagc agccaccctt ggaacgcgcg cgggacgtcg
     1441 gactgggcgt cgaacgtcac gatctcccag gcctcgggcc ggtccagcag cgcgcgcgac
     1501 agcgcgttgt tcagcgcgtg accggacttg aatgccgtgt agcgtccgat catcggatgg
     1561 ccgagcagat acaggtcgcc gatcgcgtcg agcaccttgt gcttggcgaa ctcgttgtcg
     1621 tagcgcaggc cctcgctgtt caacacgcgc gtttcgtcga gcacgatcgc gttctgcaga
     1681 ctgccgccaa gcgcgagccc ggccgagcgc atcgcttcga cgtcctgcat gaaaccgaac
     1741 gtgcgcgcgc ggctgacttc cttgatgtac gaattctcgg cgaaatcgat gatcacatgc
     1801 cggttctccg aaccgaacac cgggtgcggg aaatcgatcg tgaagtcgag cacgaagccg
     1861 tgatgcggct cgaagcgcgc ccatttgtcg ccgtcacgcg cttcgaccgg catcttgatg
     1921 cgcagatagc gtttggccgc ctcctgttcg acgatgcctg cggactgcag caggaagacg
     1981 aaaggggcgg cgctgccgtc catgatcggc agctcgggac cggcgacgtc gacgtgaaga
     2041 ttgtcgatgc ctagtccagc cagtgccgac atcagatgct cgacggtcga cacgctggcg
     2101 ccatccttgg acagcgacga cgaaagtcgg gtgtcgccga cgttgcgtgc ctcgcccgga
     2161 atcgccgccg agccaggcaa gtcgacgcga tggaatacga ttccggtttc gggatgcgcc
     2221 ggacgcagcg tcaattcgac gcgcgcgccg gtgtgcaggc cgacgccggt cgtcttgatc
     2281 ttggatttga gcgtgcgttg tttcatcatc gatgtcccct tttcggaccg gtcagcgatt
     2341 cactaaccgg ctcaaggagt tagcagacaa ttgtagcaca ggccgggaca cctacgcgtg
     2401 ctcgtccctg cctccgccgg aagcagccga tcccgttgcc ccgccgtcgc cgtcacggca
     2461 accgtcgtcg gttcgcgcct agtccgcctg cttgcgcagg aacgccggaa tgtcggaggc
     2521 atcgaacgcc gatgcaccgg acgccggcga cggcgtgcgc ccgcgccgga tgaccgcagg
     2581 cgtcttgtcc agcgaatcgt aatcgacggt ctcgatcgac atgccgacgt tgtccgtccc
     2641 ggtgcggtcg acgacgtgct cgacgacttc gagccgcggc tgccggttgc ggacggtggc
     2701 gccacccagg cccgtcgcga tcatcgtcac gcgaagctcg tccttcatcg cctcgtcgat
     2761 cacggtgccg acgatcacca tcgcatcgtc cgcagtgaac gatttgatcg tgttcatgac
     2821 ctcgtgatat tccttcatct tgaggccggc cgacgcggta atgttcacca gcacgccgcg
     2881 ggcgccggcg aggttgatct cctcgagcaa cggactcgca acggcgcgct cggcggcgag
     2941 gcgcgcgcga tcgggtccgc cgaccttggc cgagcccatc atcgccatgc cgacttctga
     3001 catcaccgtg cgcacgtcgg caaagtcgac gttgaccagg cccggattgt tgatcacctc
     3061 ggcgatcccg gcgaccgcgc tgtgcagcac gtcgttggcc gccgcgtagg catcgagcac
     3121 cgacacgtct tcgccgagca cctgcatcag cttgtcgttc gggatgatga tcagcgagtc
     3181 gacggtcttt ttcagctcct cgatgccggc ttcggcaact ctctgccgct tgccttcgaa
     3241 caggaatggc cgcgtcacga ccgcgaccgt caggatgccg cggtctttcg cgacctgcgc
     3301 gacgatcggc gccgcaccgg tcccggtccc gccgcccatg ccggcggtga tgaacagcat
     3361 gtcggcgccg tcgatcattt cgccgatccg gtctttgtcc tccatcgccg catcgcggcc
     3421 gatctccggc ctcgctccgg cgcccaggcc gcgcgtcagg ttgctgccga gctgaagctg
     3481 gatgcgcgcg gtcgagcgtt tgagcgcctg cgcatcggta ttcgctgcaa tgaactcgac
     3541 gccttgcagc tggcgttcga tcatgtgctc gatggcattg ccgccgcaac cgccgacgcc
     3601 gatgaccttg ataacggctt cctgcgggtt ctggt
//
LOCUS       DATANS010000760         2247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_79117, whole genome shotgun sequence.
ACCESSION   DATANS010000760 DATANS010000000
VERSION     DATANS010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79117"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1075
                     /locus_tag="VHT22_11320"
     CDS             86..1075
                     /locus_tag="VHT22_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX3138194.1"
                     /translation="MARPAIRYAIPLLALLAAAPVRAATADAASDYPTHPIRFILGYP
                     PGGASDAVARQLAFSLGSRMGQNIVLDNRGGAGGNIAGEIAARAAPDGYTWFLGNNGI
                     LATNLALYGKLPFDPVKDFATVTLVGTQPSILVVNPAVAATSVRELIALAKAKPGQLN
                     YASSGTGTAGHLTGELFKSAAQISYVHIPYRGGGPAVIDLVAGQVQFMFATAASVIPH
                     VKSNRLRALAVTSLKRSPSLPELPTVDESGVPKFEAVTWHGVVVPAATPRPLVLKINA
                     EINQVLALADVREKLALQGIEAQGGTPAEFAAFLKSEIPKWTKVVRDSGAKPE"
     gene            1124..2065
                     /locus_tag="VHT22_11325"
     CDS             1124..2065
                     /locus_tag="VHT22_11325"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX3138195.1"
                     /translation="MSAVFLTASFTVATAQTYPTKPIRVIVPYPAGGTSDILTRLIGA
                     KLTASWGQQIITDNRTGASGNIGAELTARAAPDGYVLMLTDIGNMVTNSLLYSKLPFD
                     VLRDFTPVITVSYSPHLLTTHPSVPVKTIKELVALAKANPGKLNFATALGGTPHLAGL
                     SFAQRTGINWVYIPTKGGAQSTFAVATGDADVMFLGILQTLPHVNNGKLKVIAVSSEQ
                     RLPNLPDTQTVAEVLPGFVTGSWQGIMAPARLPPELVAKLHSEVTRILGMPDVKEVLI
                     SQGTTPLATNPQETARWLAAEKERWAKVIREGGFKLE"
     gene            2065..>2247
                     /locus_tag="VHT22_11330"
     CDS             2065..>2247
                     /locus_tag="VHT22_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015126170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138196.1"
                     /translation="MKSSTPGNLARALAIVAACVLAACATAPPLTPTTRAELAPGGTL
                     RAGINYGNVVLATKDPK"
BASE COUNT          433 a          768 c          676 g          370 t
ORIGIN      
        1 ctggaaacgc tgccgtcgtg ccgcagcctt taccagcacg atgtcgcggc gcttaccgcc
       61 tgatgcgcgc aaattgaaag agattatggc caggcccgct atccgctatg caatcccgct
      121 actcgcattg cttgcggcgg caccggtgcg cgccgcgacc gccgacgcgg caagcgacta
      181 tccgacgcac ccgatccggt tcatcctcgg ctatccgccg ggcggtgcga gcgatgccgt
      241 tgcgcgccag ctcgcttttt cgctcggctc acggatgggg cagaacatcg tgctcgacaa
      301 tcgcggcggc gcaggcggca atatcgccgg cgaaatcgcc gcgcgcgcgg cacccgacgg
      361 ctacacgtgg tttctcggca acaacggcat cctcgcgacc aacctggcgt tatacggaaa
      421 gctgccgttc gacccggtca aggacttcgc gaccgtcacg ctcgtcggca cccagccaag
      481 catcctcgtc gtcaatcccg ccgtcgcagc aacctcggtg cgcgaattga tcgcgctcgc
      541 gaaagcgaag ccgggccagt tgaattacgc atcctcgggc accggcaccg cgggacatct
      601 caccggcgag ctgttcaagt ccgccgcgca aatcagctac gtgcacattc cgtatcgcgg
      661 cggcggcccc gcggtcatcg atctcgtcgc gggccaggtt cagttcatgt tcgccaccgc
      721 ggcctccgtc attccgcacg taaaaagcaa tcggctacgc gcgctcgcgg tgacgtcgct
      781 caagcgttcg ccaagcttgc ccgagttgcc gacggtggat gaatcaggcg tgccgaagtt
      841 cgaagcggtc acgtggcacg gcgtggtggt gcccgcggcg accccgcgcc cgctcgtgct
      901 caagatcaac gccgaaatca atcaggtgct cgccctcgcg gacgtccggg agaagctcgc
      961 cttgcagggt atcgaagcac agggcggcac gcccgctgaa tttgccgcgt ttctaaagag
     1021 cgaaataccg aagtggacga aagtcgtgcg cgattccggc gcgaagcccg agtaagccgc
     1081 accgcaacag gaggaaagca tgaaattatc agcaggcctt gccatgagcg ccgtcttctt
     1141 gacggcatcg ttcaccgtcg ctacagcgca aacgtatccg acgaaaccga tccgcgtgat
     1201 cgtgccctat ccggcgggcg gcacttccga catcctgacg cggctgatcg gcgccaagct
     1261 caccgcgagc tggggccagc agatcatcac cgacaatcgc accggcgcga gcggcaatat
     1321 cggggcggaa ctgaccgcgc gcgcggcgcc tgacggatac gtgctgatgc tgaccgacat
     1381 cggcaatatg gtcacgaact cgctcctgta ttcaaagctg ccgttcgacg tgctgcgcga
     1441 tttcacgccg gtaatcacgg tctcatattc gccgcatctc ctgacgacgc accccagcgt
     1501 gccggtaaaa acgatcaagg agctggtcgc actcgccaag gccaaccccg gcaaactcaa
     1561 ctttgcaacc gccctgggtg gcacgccgca tcttgcgggt ctcagcttcg cgcagcgcac
     1621 cggcatcaac tgggtgtata tcccgaccaa gggcggcgcg cagtcgacct tcgctgttgc
     1681 aaccggcgac gccgacgtca tgttcctggg cattctgcaa accctgcccc acgtcaacaa
     1741 cggcaaattg aaggtaatcg ccgtctcgag cgagcagcgt cttcctaacc tgccggacac
     1801 gcagacggtg gcggaagtgc tgccgggttt cgtcaccggc tcgtggcagg gcatcatggc
     1861 gcccgcacgc ctgccgcccg agctggtcgc gaaacttcac tcggaggtga cgcgcattct
     1921 cggcatgccg gacgtcaaag aggtgctgat ctcgcagggc accacaccgc tcgccaccaa
     1981 cccgcaggaa accgcgcgct ggctcgcagc cgagaaggag cgctgggcca aggtcatacg
     2041 cgagggcggg ttcaagctcg aataatgaaa tcatcgacgc cgggtaatct cgcccgcgca
     2101 ctggccatag tggccgcgtg cgtgctggca gcgtgcgcta ccgcgccgcc gctcacgccc
     2161 acgacacgcg ccgaactcgc gcctggcggc acgctgcgtg ccggcatcaa ttacggcaat
     2221 gtcgtgctcg ccacgaaaga tccgaaa
//
LOCUS       DATANS010000761         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_542137, whole genome shotgun sequence.
ACCESSION   DATANS010000761 DATANS010000000
VERSION     DATANS010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542137"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VHT22_11335"
     CDS             <1..582
                     /locus_tag="VHT22_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N C-terminal domain-containing
                     protein"
                     /protein_id="HEX3138197.1"
                     /translation="LKNACLDLLAATAAASAIALAARQYHQATNMTDRMAALTTLSLY
                     PVPERQAALDDFYRRYSDNPLVIDKWFALQATIPDPATLDRVKVLMVHPAFSIANPNR
                     VRALIGSFVQMNPTQFNRVDGAGFDFLADTALALDAKNPQVASRVTTAFRSWRALEPT
                     RRARAEAALRRIAAAPNLSRDLRDIVERSLATG"
     gene            862..>2009
                     /locus_tag="VHT22_11340"
     CDS             862..>2009
                     /locus_tag="VHT22_11340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX3138198.1"
                     /translation="MAQAVARPAYRRLLTAEPFLRRAVPVLIVAFLVTLGIAAVVDIR
                     ERLRQAVFKSADELDLAATIMTERMERLALNEKGEPLVRAFRAFENIDWPRATMGGRL
                     VLLTDASNTIIATQPALNGYVGRKLNEAIGRDPAVAPKTIIPGVSELTLADGSAIFLA
                     RRNLGSPLGELAIMQRRAVVLGEWRADTTLAVTLFSATGFVVLMLGFAFLWQSRLMRE
                     TASIEDTVHSRIDTALNCGRCGLWDWDLASGRVYWSQSMFDILGLPAHHKLLSFGEIS
                     GLVHPDDVQLYELAGELADSGSSCIDRMFRMRHASGAWVWLRTRCELVRNPGEPHPHL
                     IGIAVDITEQKRLAEESATAEMRLSDAIEAISEAFVVWDTDNRLVLCNS"
BASE COUNT          351 a          687 c          618 g          353 t
ORIGIN      
        1 ctgaagaacg cctgcctcga cctgctcgcc gccaccgcgg ccgcatcggc gatcgcactt
       61 gccgctcgcc agtaccacca ggcgaccaac atgaccgatc gcatggcggc gctgaccacg
      121 ctgtcgctct acccggtccc ggagcggcag gcggcgctcg atgatttcta tcggcgctac
      181 tccgataatc cattggtgat cgacaaatgg ttcgcgctgc aggcgaccat tccggacccg
      241 gccacgctcg accgcgtgaa ggtgctgatg gtgcatccgg cgttttcgat cgcgaatccc
      301 aaccgggtcc gcgcgctgat cggctccttc gttcaaatga acccgaccca gttcaatcgc
      361 gtcgatggcg cgggcttcga tttcctcgcc gatacggcgc ttgcgctcga cgcgaaaaac
      421 ccacaggtcg cctcgcgggt caccaccgca ttccggtcct ggcgcgcgct cgagccgacc
      481 cggcgtgcac gcgctgaggc agcgctgcgg cgcatagcag ctgcgccaaa cctgtcgcgc
      541 gacttgcgcg acatcgtcga gcgctcgctg gctactggct gatccgtgcg ccgcgcgagt
      601 caagaagtgc ctgcagaatc ggcacttcca tgacgcgaag caaaccggcg tcgcgaagtc
      661 tgaatctaaa aataatttct cctgaagatt cacggactct cgacaaatca ggcgaattgg
      721 attcagatca acagtgattc ggtcgagatg cggcacatcc ttaccgagtc aagggtgaac
      781 ggggacgtct gaagcggagg cttggggatg gcgcgcgccg acagcgcgag cgcatctgcg
      841 cattccgata ccataaaggg gttggctcaa gcggtcgcaa gacccgctta ccggcggctt
      901 ctcaccgccg aacccttcct gcgccgggcg gtgccggtgc tgatcgtggc cttcctcgtt
      961 acacttggaa tcgcagcggt cgtagacatc cgcgagcgcc tgcgccaggc ggtcttcaaa
     1021 tccgccgacg agctcgatct cgccgccacc atcatgaccg agcggatgga gcggctcgcg
     1081 ctcaacgaaa aaggcgagcc gctggtgcgc gccttccgcg ccttcgagaa catcgattgg
     1141 ccccgcgcca ccatgggcgg ccggctcgtg ctcctgaccg acgccagcaa caccatcatc
     1201 gctactcaac ctgcgctcaa cggctatgtc ggccgcaagc tcaacgaagc gatcggccgc
     1261 gacccggccg tcgcgccgaa gacgatcatt ccgggagtat ccgagctgac tctggccgac
     1321 ggctcggcca tctttctggc gcgccgcaat ctcggcagcc cgctcggtga gctcgccatc
     1381 atgcagcgcc gcgccgtcgt gctcggggag tggcgcgccg acacgacgct tgccgtcacg
     1441 ctgttctcgg ccaccggctt cgtggtgctg atgctcggct tcgccttcct ctggcagtcg
     1501 cggctgatgc gcgagaccgc cagcatcgag gacacggtgc acagccgcat cgataccgcg
     1561 ctcaactgcg gccggtgcgg gctgtgggac tgggacctcg ccagcggccg cgtctactgg
     1621 tcgcaatcga tgttcgacat tctggggcta cccgctcacc acaagctcct gagcttcggc
     1681 gagatcagcg gactggtgca tccggacgac gttcagctct acgagctcgc cggagagctc
     1741 gccgattccg gcagctcctg catcgatcgc atgttccgca tgcggcatgc gagcggcgcc
     1801 tgggtgtggc tgcgcacgcg ctgcgagctg gtgcgcaatc cgggtgagcc gcatccacac
     1861 ctgatcggca tcgcagtcga catcaccgag caaaagcgcc tcgccgaaga gagcgcgacc
     1921 gccgagatgc gcctctccga tgcgatcgag gccatttcgg aggcgttcgt cgtttgggac
     1981 accgacaatc ggctggtgct gtgcaactc
//
LOCUS       DATANS010000762         2663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_542169, whole genome shotgun sequence.
ACCESSION   DATANS010000762 DATANS010000000
VERSION     DATANS010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542169"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VHT22_11345"
     CDS             complement(<1..761)
                     /locus_tag="VHT22_11345"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HEX3138199.1"
                     /translation="MAATTESRAEDVAPKTCRNCGAPVDGAYCASCGQETALRLPTLR
                     EFVREAAGRYVALDGKFWRTVFALLARPGFLTREYLAGRRRRYIRPARLYLFATLIFF
                     AVTRLLVGPIDIFEVRPDTPEEAKYLSPDKGFNLQVDSDSVFGSPALQKRWNRFNNLS
                     KSEKAEQLGDGILRYAPYAMFVLLPAFALLLKLVYLGRGRRYPRRPRLYGEHLVFAAY
                     DHAFVFVAATVMLLVVGRTLAITVGVWIVALYLLLS"
     gene            complement(762..1544)
                     /locus_tag="VHT22_11350"
     CDS             complement(762..1544)
                     /locus_tag="VHT22_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX3138200.1"
                     /translation="MTDASEPSVVNQRAKNDALSRIRIVLCATSHPGNIGASARAMRA
                     MGLSRLTLVAPQRFPDPEADALASGATDILRGAHVVATLDEALAGAALAIGFSARRRE
                     FAGGALPVRAAAHEALAYAARGEVALVFGSEMSGLSNAELARCQLVATIPSDPSFSSL
                     NLAAAVQVAAYELRVASGAGQVWRAPAFEPATQDEIARLFEHGERTLVALDFLRPAHP
                     KRLLPRLRRLFARARLEKEEVNILRGILARIDERLQRTRKAE"
     gene            1635..2438
                     /locus_tag="VHT22_11355"
     CDS             1635..2438
                     /locus_tag="VHT22_11355"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX3138201.1"
                     /translation="MHPMLTTAVKAARRAGGIINRGARDLDRLVVTAKGPKDFVSEID
                     RAAEAAIVDTLHATYPDHAILAEEGTGEGANTASEHLWIIDPLDGTTNFLHGFPQYCV
                     SIALQHKGQITQGVIYDPVRNDLFTATRGRGAFLNDHRLRVSRRQHLKECLVGTGFPF
                     RDGSDLETYIAMMRAMMQQTAGLRRPGSAALDLAYVAAGYYDGFWEVGLNAWDVAAGS
                     LLIVEAGGLVGDLAGEGGYLRGGQIVAANPRIFAQMVKVLGPFQASLKS"
BASE COUNT          488 a          880 c          879 g          416 t
ORIGIN      
        1 gacagcagca gatagagcgc gacgatccac acgccgaccg ttatcgcaag cgtacggccc
       61 accaccaaca gcatcacggt cgccgccacg aaaacgaagg cgtggtcgta agccgcgaag
      121 accagatgct cgccgtacag acgcggacgc cgcggatatc gccggccgcg gccgaggtac
      181 acgagcttca gcagcagcgc aaacgcaggc agcagcacga acatcgcata cggcgcgtag
      241 cgcaggatgc cgtcacccag ctgctcggcc ttttcgctct tggagagatt gttgaaacga
      301 ttccaccgct tttgcagcgc cggcgatccg aatacgctgt ccgagtcgac ctgcagattg
      361 aagcccttgt ccggcgacag atatttggcc tcctccggcg tatcgggacg gacctcgaaa
      421 atatcgatcg gccccaccag cagtcgcgtc acggcgaaaa atatcagcgt cgcgaacagg
      481 tacaggcgcg ccggacggat gtagcggcgg cggcgcccgg ccaggtactc gcgcgtcagg
      541 aatccggggc gcgcgagcag cgcgaagacc gtgcgccaga acttgccgtc aagtgccaca
      601 tagcgtccgg ccgcttcgcg cacgaactcg cgcagcgtcg gcaaacgcaa cgccgtctcc
      661 tggccgcaac tcgcgcaata cgcgccgtcg accggcgcgc cgcagttgcg gcaggtcttc
      721 ggtgcgacat cctcggctcg ggattcggtc gtcgccgcca ttcactccgc cttgcgcgtg
      781 cgctgaaggc gctcgtcgat ccgcgccagg ataccgcgca ggatgttcac ttcctccttc
      841 tccaggcggg cgcgcgcgaa cagccgacgc aagcgcggca acaggcgctt cggatgggcg
      901 ggccgcagaa aatcgagcgc gaccagcgtt cgctcgccgt gctcgaacaa gcgcgcgatc
      961 tcatcctgcg tcgcgggctc gaacgccggc gcgcgccaga cctgcccggc gcccgaggcg
     1021 acgcgcagtt catacgcggc cacctggacc gcagccgcga gattgagcga cgaaaacgac
     1081 gggtccgacg gaatcgtcgc gacgagctgg cagcgcgcga gctcggcatt ggagagtccg
     1141 gacatctcgc tgccgaagac caacgcgact tcgccgcgcg cggcataggc caacgcctcg
     1201 tgggccgccg cgcgcaccgg cagcgcgccg ccggcgaact cgcgccgccg cgccgagaat
     1261 ccgatcgcca gcgccgcacc ggccagcgcc tcgtcaagcg tggcgacgac gtgtgcccca
     1321 cgcaggatgt cggtcgcgcc ggaagcgagc gcgtcggcct cgggatcggg aaagcgctgc
     1381 ggcgcaacca gggtcaatcg cgagagaccc atcgcgcgca tcgcgcgcgc ggaggcgccg
     1441 atgttgcccg gatggctggt cgcgcacagg acgatgcgga tgcgcgagag cgcatcgttt
     1501 ttcgctcgct gattgacgac ggatggctcg gatgcgtccg tcatgcgcgt cgagaggctt
     1561 cgtttgcggg tagaatgcgc gacgattgtt ctttatgcgc cgctcgcgcg tcagcgcaaa
     1621 agcggtgaac ccgaatgcac ccgatgctca ccaccgcggt caaggccgcg cgccgcgccg
     1681 gcggcatcat caatcgcggc gcgcgcgatc tggaccggct tgtcgtcacc gccaagggtc
     1741 cgaaggattt cgtcagcgag atcgaccgcg ctgccgaagc ggcgatcgtc gatacgctgc
     1801 acgccaccta tcccgaccat gcgattctag cagaggaagg caccggcgaa ggcgcgaaca
     1861 cggcatccga gcatctgtgg atcatcgatc cgctcgatgg cacgaccaat ttcctgcacg
     1921 gctttccgca gtactgcgtt tcgatcgcct tgcagcacaa gggacagatc acacagggcg
     1981 tgatttacga tccggtgcgc aacgacctct ttaccgcaac gcgcggccgc ggcgcatttc
     2041 tcaacgatca tcggctgcgc gtgtcgcgcc gccagcatct gaaagagtgc ctggtcggca
     2101 ccggctttcc gtttcgcgac ggcagtgatc tggaaaccta tatcgcgatg atgcgcgcga
     2161 tgatgcagca gacggcgggc ctgcgccggc ccgggtcggc ggcgctggac ctggcctatg
     2221 tcgccgccgg gtattacgac ggattctggg aggttgggct caatgcctgg gacgtggcgg
     2281 ccggcagcct gcttatcgtg gaggccggcg gcctggtcgg cgacctggct ggcgaaggcg
     2341 gctatttgcg cggcggccag atcgtggccg cgaatccgcg gatattcgcc caaatggtca
     2401 aggtgctggg gcctttccaa gcgtcgctca agtcgtagtc gacaagaaag gctgaggttt
     2461 tcgggcaccc cgcccgcccg acccttccgg ggcgtatcgt tgtttcccgg cgctaaatcc
     2521 tgacaaagga aggatttggc gcgcaatctt catgacaatt ccaatgtcat tgtgtacgat
     2581 gaggcgcact ggcaatgcgc gccgcggggc ggcagccgtt gcgggaaaca tccgggactg
     2641 ggagcgaccg atggttcgac gat
//
LOCUS       DATANS010000763         4289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_208064, whole genome shotgun sequence.
ACCESSION   DATANS010000763 DATANS010000000
VERSION     DATANS010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208064"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2545)
                     /locus_tag="VHT22_11360"
     CDS             complement(<1..2545)
                     /locus_tag="VHT22_11360"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX3138202.1"
                     /translation="MTLGKTFEPRAIEAKWYPYWEGHGLFKPSLRPGVESFCIQLPPP
                     NVTGTLHMGHAFQQTLMDVLIRYHRMCGDNTLWQVGTDHAGIATQIVVENQLKAEGKS
                     RQELGREKFIERVWAWKQASGSTITNQMRRLGASCDWSRERFTMDEGLSNAVIETFVR
                     LYEDGLIYRGKRLVNWDPKLGTAVSDLEVENEEATGKLWEIRYPLADGSGQLTVATTR
                     PETMLGDVAVAVNPDDTRFAHLVGKEVALPLAGRRIPVIADSYVDKEFGTGVVKITPA
                     HDFNDWDVAQRHGLAPITIFTLQATVNDNAPAKYRGLDRYAARKLVLSDLEAAGLLAG
                     EKSHKMVVPRCGRSGEIVEPMLTDQWFVAMTKPAPKTHAWFPGKSFKDICLQAVNASL
                     PDPRGGAPASIRFVPEHWTSTYNDWLDNVRDWCISRQVWWGHRIPAWYDDAGKVYVAR
                     NTADAAKLAGKPASALRQDDDVLDTWFSSALWCHATLGWPQQTPELRTFLPSSVLITG
                     FDIIFFWVVRMIMMTTYFTGERPFRDVYINSIVRDEEGQKMSKSKGNILDPLDLIDGA
                     DVETLVATSTSGLMLEKQKQSIEKRTRKQFPNGIPAFGADAVRFTFASLATFGRTLNF
                     DLSRCDGYRNFCNKLWNATRFVLMNVAGKDVGLDDAAPISYSLADRWIVAELQNTEEE
                     VNTQLADYRFDLAAKAIYEFVWNQYCDWYVELAKVDLASGDEGAQRGTRRTLVRVLET
                     TLRLAHPIIPFITEELWQTLAPLAGKHGDSISVQPYPKADFARLDPKASTAIATLMAL
                     VESCRSLRGQMNISPAARVAALIAGDVAGVGAGALLDYLKALAKLSDARI"
     gene            complement(2629..3687)
                     /gene="aroF"
                     /locus_tag="VHT22_11365"
     CDS             complement(2629..3687)
                     /gene="aroF"
                     /locus_tag="VHT22_11365"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX3138203.1"
                     /translation="MIIVMQPEAGDAEIAAVEARIRAAGLEVHVSRGTERTLIGAIGD
                     ERKLDPEMFDPLPGVERSMHILKPYKLVAREWHKASTIVDVAGVKIGANAVQVIAGPC
                     SVETQPQMDAAADGVAAAGARLMRGGAFKPRTSPYAFQGKGVDGLAMLRDAAARHGLP
                     IVTELMDVRMLDAFLEHKVDVIQIGTRNMQNFDLLKEVGRVDVPVILKRGMSATISEW
                     LMAAEYIAAGGNHRIMLCERGIRTFETAYRNVLDVTAVAVLKRETHLPVIVDPSHAGG
                     KAWMVPALSCAAVAAGADALLIEVHPCPSEAWCDADQALTPDEFAGLMNRLDALALAL
                     GRRIDRQAAKEESAALAR"
     gene            complement(3938..>4289)
                     /locus_tag="VHT22_11370"
     CDS             complement(3938..>4289)
                     /locus_tag="VHT22_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138204.1"
                     /translation="PEPEPEPEPEPEPESPPAAAPAPVVVDDSAERAAKAAAEEERWR
                     ALAEQVSMQVLQRVDLFTDTGLKTELAGHLQPIVERASAELVKAITEHVGGILRTYVA
                     EAIEREIAQWRREH"
BASE COUNT          717 a         1412 c         1363 g          797 t
ORIGIN      
        1 cgatccgcgc atcggaaagc ttcgccagcg ccttcaggta gtcgagcagt gcgcccgccc
       61 ccacaccggc gacatccccg gcgatcagcg ccgcgacgcg cgcggccgga gagatgttca
      121 tctggccgcg cagtgagcga caggactcga ccagcgccat cagcgtggcg atggccgtac
      181 tcgccttcgg atccagcctg gcgaagtcgg ccttgggata aggctgaacc gaaatcgaat
      241 cgccatgctt gccggcgagc ggcgcgagcg tttgccagag ctcctcggtg ataaagggaa
      301 tgatcggatg cgcaaggcgg agcgtcgtct ccagcacgcg gaccagcgtg cgccgcgtgc
      361 cgcgctgcgc gccctcgtcg ccacttgcaa gatcgacctt ggccaactcg acgtaccaat
      421 cgcagtactg gttccaaacg aactcgtaga tcgccttggc ggcaaggtcg aaccggtagt
      481 ctgcgagctg cgtgttgact tcctcttcgg tgttctgcag ctcggcgacg atccaccgat
      541 cggcaagcga atacgatatc ggcgccgcat cgtcgagtcc gacgtccttt cctgcgacat
      601 tcatcagtac gaatcgcgtc gcgttccaca gcttgttgca gaaattgcga tagccatcgc
      661 agcgcgaaag atcgaagttg agcgtgcggc cgaacgtagc gaggctggcg aaggtgaagc
      721 gcaccgcgtc ggccccgaac gccggtatgc cgttcggaaa ctgcttgcgc gtacgctttt
      781 cgatcgactg cttttgcttc tccagcatca gccccgacgt gctcgtggcg accagcgtct
      841 cgacgtcggc gccgtcgatc aggtcaagcg gatcgaggat attgcccttc gacttcgaca
      901 tcttctgccc ttcctcgtcg cgcacgatcg aattgatgta cacgtcgcgg aacggccgct
      961 cgccggtgaa ataagtcgtc atcatgatca tgcggacgac ccagaagaag atgatgtcga
     1021 agccggtgat cagcaccgag ctcggcagga acgtccgcaa ctcgggcgtt tgttgcggcc
     1081 agcccagcgt cgcatggcac cacagcgccg acgagaacca ggtgtcgagg acgtcgtcgt
     1141 cctgccggag cgcgctggct ggcttgcctg ccagcttcgc cgcgtcggcc gtattgcgcg
     1201 ccacgtagac cttgcccgcg tcgtcgtacc acgccgggat gcggtggccc caccacacct
     1261 ggcgcgaaat gcaccagtcg cggacgttgt cgagccagtc gttgtacgtc gacgtccagt
     1321 gctcgggcac gaagcggatc gacgccggcg cgccgccgcg cggatcgggc aggcttgcat
     1381 tgaccgcctg caggcagatg tccttgaacg atttgccggg aaaccaggcg tgcgtcttcg
     1441 gcgcgggctt ggtcatcgcg acgaaccact gatctgtcag catcggctcg acgatttcgc
     1501 cgctgcgccc gcagcgcggt acgaccatct tgtgcgactt ttcgccggcc agcagtcccg
     1561 cggcctcgag gtcggagagc acgagcttgc gcgcggcata gcgatcgagg ccacgatact
     1621 tggccggcgc gttgtcgttc accgtcgcct gaagcgtgaa aatggtgatc ggtgcgaggc
     1681 cgtgccgctg ggcgacgtcc cagtcgttga agtcgtgcgc cggcgtgatc ttgaccacgc
     1741 cggtgccgaa ttccttgtcg acgtagctgt cggcgatgac cgggatcctt cgcccggcga
     1801 gaggcaacgc gacctccttg ccgaccagat gcgcgaagcg cgtgtcgtcc ggattgaccg
     1861 cgaccgcgac atcacccagc atcgtttccg gacgtgtcgt ggccaccgtc agctggcccg
     1921 agccgtcggc gagcgggtag cggatttccc agagcttgcc ggtcgcttcc tcgttctcga
     1981 cctccagatc ggagaccgcg gtaccgagct tcgggtccca gttgacgagt cgcttgccac
     2041 ggtagatcag gccatcctcg taaaggcgga caaaggtttc gatcaccgcg ttcgacaggc
     2101 cttcgtccat cgtgaagcgc tcgcgcgacc agtcgcacga cgcgccgagt cgccgcatct
     2161 ggttggtgat cgtcgatccc gacgcctgtt tccacgccca cactctttcg atgaactttt
     2221 cccggccgag ctcctgccgc gacttccctt cggccttcag ctggttctcg acgacgatct
     2281 gcgtcgcgat gccggcgtga tcggtgccta cctgccagag ggtgttgtcc ccgcacatgc
     2341 ggtgatagcg gatcagcaca tccatcagcg tctgctggaa cgcgtgtccc atgtgcagcg
     2401 tgccggtgac gttcggcgga ggaagctgga tgcagaacga ttccacaccg ggtcgcaacg
     2461 acggcttgaa caagccgtgg ccttcccagt acggatacca tttcgcctcg atggcgcgcg
     2521 gttcgaaggt cttgcctaaa gtcatcgcga ttgccggaat atctcgtaaa aacatgacat
     2581 tatagacgaa tccgacgcac cttgcgcgct tggcatgctc acgcccgctc agcgcgcgag
     2641 cgcggcggac tcctctttcg ctgcctgacg atcgatgcga cgtcccagcg ccagggcgag
     2701 tgcatcgagc ctgttcatca gccccgcgaa ctcgtccggc gtcagcgcct gatcggcgtc
     2761 gcaccacgct tcggacgggc acgggtgcac ttcgatcagc agcgcgtcgg cgccggccgc
     2821 gaccgcggcg cacgacagtg ccgggaccat ccaagccttg ccgccggcgt gcgagggatc
     2881 gacgatgacc ggcagatgcg tctcgcgctt gagcactgcg accgcagtga cgtcgagcac
     2941 gttgcgatac gcggtctcga acgtgcggat gccgcgctcg caaagcatga tgcggtgatt
     3001 gcctccggcg gcgatgtact cggcagccat cagccattcg ctgatcgtcg cgctcatccc
     3061 gcgtttcaat atgaccggca cgtcgacccg cccgacttcc ttcagcaagt cgaagttctg
     3121 catgttgcgc gtgccgatct ggatgacatc gaccttgtgc tcgaggaagg catccagcat
     3181 ccgcacatcc atcagttcgg tgacgatggg cagcccgtgt cgcgctgccg cgtcccggag
     3241 catcgcaagt ccgtcgacac ccttgccctg gaatgcgtaa gggctggtgc gcggcttgaa
     3301 tgcgccgccg cgcatcaagc gcgcgccggc tgccgcgacg ccgtcggccg cggcgtccat
     3361 ctgcggctgc gtctcgaccg agcacgggcc ggcgatcacc tgcacggcgt tcgcgccaat
     3421 cttgacgccg gcgacgtcga cgatcgtgct cgccttgtgc cattcccgcg ccaccagctt
     3481 gtatggcttc aggatgtgca tcgagcgctc gacgccgggc agcggatcga acatctcggg
     3541 atcgagcttg cgctcgtcgc cgattgcgcc gatcagcgtc cgctcggtgc cgcgcgagac
     3601 atggacctcg aggccggcgg cgcgaatcct ggcctcgacc gcggcgattt cggcgtcgcc
     3661 ggcttcgggt tgcatcacga taatcattgc aggctcctta aaaaaaattc gggcggcctt
     3721 tgggggccgc ccgacttgtc ttgaaacttg gtgcttcgaa ctatcggcga gcgcggcccc
     3781 gggagattgt cccgaaatag taaaagccgg cgtacccggt cacgcctgca gccttgcctt
     3841 gacgggcgga aacgaatcgg gcgtgcgacg aattcatgat tcggagtcta gcacggctcg
     3901 caaccgggag ggcaacgggc gtccttgcga cgcgacttca gtgctcgcgc cgccactgcg
     3961 cgatctcccg ctcgatggct tcggccacat aggtgcgcaa tataccgccg acgtgctcgg
     4021 tgatcgcctt gaccagctcg gcgctcgcgc gctcgacgat gggctgcaga tgcccggcga
     4081 gctcggtctt gaggccagtg tcggtaaaca gatcgacccg ctgcaatacc tgcatcgaaa
     4141 cctgctcggc cagcgcgcgc caccgctctt cttcggcggc tgccttcgcc gcgcgctcgg
     4201 ccgagtcgtc gacgacgacc ggagcgggag cggcggcagg cggcgactcg ggttcgggtt
     4261 cgggttcggg ttcgggctcg ggttcgggt
//
LOCUS       DATANS010000764         1931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_374572, whole genome shotgun sequence.
ACCESSION   DATANS010000764 DATANS010000000
VERSION     DATANS010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374572"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VHT22_11375"
     CDS             complement(<1..554)
                     /locus_tag="VHT22_11375"
                     /inference="COORDINATES: protein motif:HMM:NF022800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEX3138205.1"
                     /translation="MTPGVPPAPRSWRVAAAVALLAALALFALTLAHWGWIWFGPQPI
                     VIATPAPETDLARRTAEAHLFGPAGAAPANAPASADLGDIRLLGVFAQADGKGYALLR
                     AGTRAAMLVTAGQDISPGMRIESVRPDGVSVLQGGVRRELALRTQASADRPRVAAAAA
                     GPAACAIPPGFKGPVVRLNAELLGG"
     gene            585..1799
                     /gene="dapC"
                     /locus_tag="VHT22_11380"
     CDS             585..1799
                     /gene="dapC"
                     /locus_tag="VHT22_11380"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEX3138206.1"
                     /translation="MPSSLKPLNPQLALLQPYPFERLRQLFAGVTPAAATSPISLSIG
                     EPRHPTPQFIRDALTAGSVGLSNYPTTAGAPALREAIAAWLSRRHRLPTLDPMSQVLP
                     VLGSREALFAFAQTVVDASRVGATVIVPNPFYQIYEGATLLAGAAPHYVNSLAANGFA
                     PQWREIGDAVWARTQLVYVCSPDNPTGRVATHDEWRTLFDLSDRHGFVIASDECYSEI
                     YCDEAHPPLGALAAAVALGRDGFPRLVVFGSLSKRSNCPGLRSGYVAGDAALIKAFLL
                     YRTYHGSAMSPAVAAASIAAWSDEAHVRANRALYAQKFAALAPRVNAVLTAPTPEAAF
                     YLWANVGGDDAEFARRLYADQGVSVLPGSYLGRTVGGVNPGAGYIRIALVAEFDECAE
                     GIDRLVRFARND"
BASE COUNT          295 a          679 c          642 g          315 t
ORIGIN      
        1 ccgccgagga gctctgcatt caggcgcacg acaggccctt tgaacccggg cgggattgcg
       61 caggcggccg gaccggccgc tgccgcagcg acgcgcggcc ggtcggcgct cgcctgcgta
      121 cgcagcgcaa gttcgcgtcg aacgcccccc tgcagcacgc tgacgccgtc cgggcgaaca
      181 ctttcgatgc gcatgccggg agagatgtcc tgacccgctg tcaccagcat cgcggcgcgc
      241 gttccggcgc gcagcagcgc gtagcccttg ccgtctgctt gtgcgaagac gccgagcagg
      301 cgaatatcgc cgaggtctgc actggccggc gcattcgccg gcgcggctcc ggccgggccg
      361 aacaagtggg cctccgcagt gcggcgcgcg agatcggttt caggtgcggg cgtggcgatg
      421 actatcggct gcggtccgaa ccagatccaa ccccagtgcg cgagcgtcag agcgaagagc
      481 gccagtgcgg cgagcaatgc gaccgccgcg gcgacgcgcc agctccgcgg cgccggcggc
      541 actccggggg tcacgtataa tccggcattg ctcgccacga ttcgatgcct tcgagtctca
      601 agccgttgaa cccgcagctc gcgttgttgc agccctatcc gttcgagcgc ctgcgccaat
      661 tgttcgcggg cgttacgccc gccgcagcga cgtcgccgat cagcctgtcg atcggcgagc
      721 ccaggcaccc gacgccgcag ttcattcgcg acgcgctgac cgcgggcagc gtcggccttt
      781 caaattatcc cacaacggct ggagcacccg cgctgcgcga ggcgatcgcg gcatggctct
      841 ctcggcgtca tcgcctgccc acgctggatc cgatgtcgca ggtcttaccg gtactcggca
      901 gccgggaggc attgttcgcg tttgcccaga cggtcgtcga tgcgagccgc gtcggcgcga
      961 cggtgatcgt tcccaatccg ttctatcaga tctacgaagg cgcgacgctg cttgcgggag
     1021 cagcgccgca ctacgtcaat agccttgcag cgaatggctt cgcgccgcaa tggcgcgaga
     1081 tcggcgacgc ggtctgggcc cgcacgcaac tcgtctacgt gtgctcgccg gacaatccca
     1141 ccggccgcgt cgcaacgcac gacgagtggc ggacgctgtt cgatctatcg gatcgccatg
     1201 gcttcgtgat cgcttccgat gaatgctatt cggagatcta ttgcgacgaa gcgcatcccc
     1261 cgctcggcgc gctcgcggcg gccgtagcgc tcggtcgcga cggctttcca cggcttgtcg
     1321 tcttcggcag tttatccaag cggtcgaact gcccggggct gcgctcgggt tacgtcgccg
     1381 gcgacgcggc gctgatcaaa gccttcctgc tgtaccgcac ctaccacggg tcggcgatgt
     1441 cacccgcggt ggccgccgcc agcatcgcgg catggagcga cgaagcgcat gtgcgagcca
     1501 atcgcgcgct gtacgcgcaa aaattcgcgg cgctggcgcc gcgcgtcaac gcggtgctca
     1561 ccgcgcccac gcccgaagcg gccttctatc tgtgggccaa cgtcggcggc gacgatgccg
     1621 agttcgcccg gcgcctctac gccgaccagg gcgtcagcgt ccttcccgga agttacctgg
     1681 gtcgaacggt cggcggcgtc aatccgggag ccggctacat tcgcatcgcg ctggtcgccg
     1741 aattcgacga atgcgccgaa gggatcgacc gactcgtccg gttcgcgcgc aacgactaga
     1801 aagacggccc gtccgtctcc gcccgcgccg ttttatcgtg gccgggggcg gcccgtcgcc
     1861 tgaccgagtc gacggcgacg gcgcgcgtac aatcggcggc catggcgact atgagcacat
     1921 cctccggcga c
//
LOCUS       DATANS010000765         1582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_500507, whole genome shotgun sequence.
ACCESSION   DATANS010000765 DATANS010000000
VERSION     DATANS010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500507"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1450)
                     /gene="mnmG"
                     /locus_tag="VHT22_11385"
     CDS             complement(<1..1450)
                     /gene="mnmG"
                     /locus_tag="VHT22_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX3138207.1"
                     /translation="MRFPDEFDVIVVGGGHAGTEAALAAARLGVRTLLLTQSIETLGQ
                     MSCNPAIGGIGKGHLVKEIDALGGAMAQAADRAGIQFRTLNASKGPAVRATRAQADRV
                     LYKQAIRSTLENQPNLTLFQQEVADLIVEGEQVRGVVTQTGIEFRARAVVLTVGTFLG
                     GKIHVGLTNYQGGRAGDPPSNRLAERLRALPFRVGRLKTGTPPRIDGRTIDYTGLAEQ
                     PGDEPVPVFSYLGRRSDHPRQVSCHITATNERTHEIIRAGCDRSPMFTGVIEGVGPRY
                     CPSVEDKVHRFADKTSHQIFIEPEGLTTHEVYPNGISTSLPFDVQLELVRSIAGFERA
                     HVTRPGYAIEYDYFDPRDLQYSLETKFIAGLFFAGQINGTTGYEEAAAQGILAGINAA
                     LRVQDRAAWWPRRDEAYIGVLVDDLITRGTSEPYRMFTSRAEYRLLLREDNADLRLTP
                     RGRELGLVSDERWELFAAKRDRTTAEVERLERA"
BASE COUNT          256 a          529 c          521 g          276 t
ORIGIN      
        1 cggcgcgttc cagccgttcg acttcggctg tcgtgcgatc gcgcttcgcg gcgaacagct
       61 cccagcgctc atcactgacg agcccgagtt cgcggccgcg cggcgtcagt cgcaggtccg
      121 cgttgtcttc gcgcagcagc aagcgatatt ccgctcggct ggtgaacatg cgatacggct
      181 cgctcgtgcc gcgagttatc agatcgtcga cgagcacgcc gatgtaagcc tcatcgcgtc
      241 gcggccacca cgctgcgcga tcttgcacgc gcagcgccgc attgatgccg gcgaggatgc
      301 cttgcgccgc ggcctcttcg tacccggtcg taccgttgat ctgacccgcg aagaacagtc
      361 cggcgatgaa cttcgtctcc aggctgtact gcaggtcgcg cggatcgaaa tagtcgtact
      421 cgatcgcgta gcccggccgc gtgacgtgcg cgcgctcgaa gcccgcgatc gaacgcacga
      481 gctcgagctg cacgtcgaag ggcaggctcg tcgaaatgcc gttcggatag acctcgtgcg
      541 tcgtcagccc ttccggctcg atgaaaatct gatgcgaggt cttgtccgcg aagcgatgga
      601 ctttgtcctc gaccgacggg cagtaacgcg gaccgacgcc ttcgatcacg ccggtgaaca
      661 tcggcgagcg atcgcagccg gcgcggatga tttcgtgcgt gcgctcgttg gtcgccgtga
      721 tgtggcaaga cacctggcgc ggatggtcgc tgcgtcgccc gagataggag aacaccggca
      781 cgggctcgtc gcccggctgc tcggcgagtc cggtgtagtc gatcgtgcga ccgtcgatgc
      841 gcggcggcgt gccggtcttg agccggccga cgcggaaggg cagagcgcgc agccgctcgg
      901 ccaggcgatt cgaaggcgga tcgccggcgc gaccgccctg gtagttcgtg agaccgacat
      961 ggatcttgcc gccgaggaac gtgccgacgg tcagcaccac tgcgcgcgcg cggaactcga
     1021 tacccgtctg cgtgacgacg ccacgcacct gctcgccttc gacgatcagg tcggcgactt
     1081 cctgctggaa cagcgtgagg ttcggctggt tctccagcgt cgaacggatc gcctgcttgt
     1141 acagaacgcg atctgcctgc gcacgcgtcg cgcgtactgc cggacctttg ctggcattga
     1201 gagtacggaa ctggatgccg gcgcgatcgg ccgcctgcgc catcgcaccg ccgagggcat
     1261 cgatctcctt gaccaagtgc cccttgccga tcccgccgat cgccgggttg cagctcatct
     1321 gcccgagcgt ctcgatgctc tgcgtcagca gcagcgtacg cacgcccagg cgcgccgcgg
     1381 ctagagcagc ctcggtgccg gcatgaccgc cgcctactac gatgacgtcg aattcgtcgg
     1441 gaaaacgcat gggatccgcc cggttcaggg ggcgcgtatt gtggtttgga cttgccagga
     1501 cggcaagaag tcgggctgta ggtcgggctt cagcccgact ccggcccccg aacccgccgc
     1561 aacaacacca gcgccatcgc ca
//
LOCUS       DATANS010000766         6646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_542275, whole genome shotgun sequence.
ACCESSION   DATANS010000766 DATANS010000000
VERSION     DATANS010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 6646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6646
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542275"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1002)
                     /locus_tag="VHT22_11390"
     CDS             complement(19..1002)
                     /locus_tag="VHT22_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3138208.1"
                     /translation="MLAFIIRRILQSFAVMAVVAFIAFALFNFTGDPVTFMVGQDATQ
                     EERARLRSDLGLDQPFYVQFSRFVGRAAQGDFGLSLRQGRKVSTLLKERLPATLELAF
                     VAALLALALGVPAGVYTALRRDSWLSQLLLAVSLIGVSLPTFLIGILLILVFAVILGW
                     LPSFGRGDTVALGWWTTGFLTKGGLKALILPAITLSLFQMTLIMRLVRAEMLEVLRTD
                     YIKFARARGLTDRTVHFGHALKNTLVPVITITGLQLGAIIAFAIITETVFQWPGMGLL
                     FIQAVSFADVPVMAAYLCFIALVFVLINLVVDLLYYAVDPRLRIERLAVAQ"
     gene            complement(1145..2167)
                     /locus_tag="VHT22_11395"
     CDS             complement(1145..2167)
                     /locus_tag="VHT22_11395"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX3138209.1"
                     /translation="MSAGAPLLEVDRLTRHYAVSRGPFRAKAVVRALDGVSFTVRAGE
                     TLAVVGESGCGKSTLARQVTMIERPTSGGLKLDGIDVVDADNATLKRLRPMVQMVFQN
                     PYASLNPRKKVGTLLEEPLAINAGIDKAARRAAARAMMAKVGLRPEHYRRYPHMFSGG
                     QRQRIAVARALMLNPKLVVADEPVSALDVSIQAQVLNLMMDLQQEMGIAYLFISHNLQ
                     VVRHIADAIAVMYLGKIVEYGSKADVFARPAHPYTRSLLASTPRLRRDASRTGSAVTG
                     AAIDTRRERAAIRGEPPSPLSQRPGCAFHTRCPYAIERCRVEVPPLEPLGKVLVACHR
                     AAEIAG"
     gene            complement(2164..3204)
                     /locus_tag="VHT22_11400"
     CDS             complement(2164..3204)
                     /locus_tag="VHT22_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX3138210.1"
                     /translation="MALLEIEGLTVEFGSVAAPLMAVDDVDLTIDAGEIVGCVGESGS
                     GKSVTALALMGLVDFPGRVRARRMEFAGRQLLSLSAAERRALTGKDIAMIFQDPLASL
                     NPCFTVAFQLTETLRMHGTPEERGSAKLRRARALQLLQQVEIPDPAARLGAFPHQLSG
                     GMAQRVMIAMAIACNPRLLIADEPTTALDVTVQAQVLDLLLRLQQERGMALLLITHDL
                     AVVAETAQRVIVMYAGEQVETGPVPQIFDTPQHPYTKALLDALPEHNVDRARLKAIPG
                     VVPGQYDRPAGCLLAPRCDFALARCWVDRPPLAGPAARKVRCHFALDAAGRPTHGWAE
                     EAAATRAMAGSR"
     gene            complement(3207..4109)
                     /locus_tag="VHT22_11405"
     CDS             complement(3207..4109)
                     /locus_tag="VHT22_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3138211.1"
                     /translation="MSEVTAIAADVPPAVPGTLREFWLAYTQSRGAIIGFALIVVLVV
                     LAIGANVIAPHSPYEQFREFTLAPPAWHDGGSSRFLLGTDPVGRDILSRLIHGTRLSL
                     LIGVISVAISLSLGVVLGLAAGYFRGLVETTIMRLMDVMLSLPSLLLAIAVVAILGPG
                     LINAMYAIAIVLLPHYVRLTRAAVIAEAAKEYVASSRIAGAGPLRLMFNTILPNCAAP
                     LIVQATLGFSNAILDAAALGFLGLGAQPPTPEWGSMLADALEFIQRASWVVTFPGLAI
                     LITVLAFNLMGDGLRDALDPKLKR"
     gene            complement(4106..5113)
                     /locus_tag="VHT22_11410"
     CDS             complement(4106..5113)
                     /locus_tag="VHT22_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3138212.1"
                     /translation="MLTFILRRAALIVPTFFGVTLLAFALIHLIPGDPVENLSGERGM
                     DPARRLRLLHEFGLDRPLPVQYADYIGQVLRGDLGTSLTTHEPVLREFLTLFPATVEL
                     AFFAMLFAVAVGLPAGIIAALKRNTVADYAVMGVSLTGYSMPIFWWALLLILFFSVQL
                     GWTPVSGRIAVEFDVPASTGFMLIDTLRAGDAGAFRSAVSHLLLPTIALGTIPLAVIA
                     RMTRSSMLEVLREDYVRTARAKGIPYRQVVLVHALRNALIPVITVIGLQVGLLLSGAI
                     LTETIFSWPGVGNWLVHGVQSRDYPVVQGGILLIATIVISVNLIVDLLYGVINPRIRH
                     A"
     gene            complement(5216..>6646)
                     /locus_tag="VHT22_11415"
     CDS             complement(5216..>6646)
                     /locus_tag="VHT22_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3138213.1"
                     /translation="EFETGTTNIIPALAESWTVSPDSKVVTFKLRHGVKFHSNANFKP
                     TRDFNADDVIFSWTRMADDNHPFHKMTAGQTYAYFDDMGMKNIVDKVEAVDKYTVRFT
                     LKRPEAPFLADMAMDFASINSAEYFETMMKKGTPNAADIYPIGTGPFEYVSYQKDATI
                     RYKAFDQYWKGRPKIDNLIFSITRDATARYAKLKTGECQAMAFPKPADLAEMQKDPQL
                     NVLQKEGLNVGYIAFNVEKKPFDNKLVRQALNMATNKDAILKAVYQGQGQVAKNPIPP
                     ILWSYNNAVQDYAYDVAKAKQLLAQAGYPNGFEVDLWYLPVTRPYNPDGKRMAEMIQA
                     DWDKIGVKAKLVTYEWAEYRKRSKTGEQAVMMFGWSGDNGDPDNFFVPLLGCEAVKGG
                     GNVARWCNKPFEDLVIKAAQTPNKADRAKFYEQAQVIFKEEAPWITLAHSVRFDPIRK
                     EVKGYKMDATAHHYFTNADVVRDNAQ"
BASE COUNT         1237 a         2189 c         2148 g         1072 t
ORIGIN      
        1 ctgcagactt gtggcgactc attgcgccac cgcgaggcgt tcgatgcgca atctcggatc
       61 gaccgcatag tacaggagat cgacgaccag attgatcagc acgaacacca gtgcgataaa
      121 gcacaagtag gccgccatca ccgggacatc ggcgaagctg accgcctgaa tgaacagcaa
      181 gcccatgccc ggccactgga ataccgtctc ggtgatgatc gcgaaggcaa tgatcgcgcc
      241 gagctgcaga ccggtgatcg tgatgaccgg aaccagcgtg tttttaagcg catggccgaa
      301 atgcacggtg cggtcggtga gtccccgcgc ccgcgcaaac ttgatgtagt cggtgcgcag
      361 cacctcgagc atttcagccc gcaccagccg catgatcaag gtcatctgga acagcgacaa
      421 ggtgatcgcc ggcaggatca gcgccttgag cccgcctttg gtcaggaagc cggtcgtcca
      481 ccagcccagc gccacggtgt cgccgcgacc aaacgaaggc agccagccga ggatcaccgc
      541 aaatacgagg atcagcagga tgccgatcag gaacgtcggc agcgatacac cgatcaggga
      601 caccgccagc agcagttggg acagccacga gtcgcgccgg agcgccgtgt agacaccggc
      661 cgggacgccg agcgccagcg ccagcaaagc cgcgacgaaa gcgagctcca gcgttgccgg
      721 caggcgctcc ttgagcaagg tcgacacctt gcgcccctgg cgcagcgaca ggccgaagtc
      781 cccctgagcc gcgcggccga cgaagcggct gaactgcacg taaaaaggct ggtcgaggcc
      841 gagatcggag cgcagtctgg cgcgctcttc ctgcgttgcg tcctgcccga ccatgaacgt
      901 caccgggtcg ccggtaaaat tgaacagggc aaacgcaatg aacgccacca ccgccatgac
      961 ggcgaacgat tggaggatgc ggcggatgat gaaggcaagc acgtaaaagc ggctgcgctc
     1021 acgcccggac tggtgaaaga ccgcgtcgcg acgcccgccg atgatgccta aagccctggg
     1081 cagcgtcaat caggcgcgcc gccgcgacct cggccgtaga aaacggggtg gacaggagtc
     1141 ctggctagcc agcgatctcg gcggcgcgat ggcaggcgac cagcaccttg cccaatggct
     1201 cgagcggcgg aacctccacc cggcagcgtt ctatcgcgta cgggcagcgc gtgtggaacg
     1261 cgcagcccgg ccgttgcgac agcggcgacg gcggctctcc ccgaatcgcg gcgcgctcgc
     1321 gccgcgtgtc gattgccgcg cccgtaaccg cgctccccgt gcgcgacgcg tcgcggcgca
     1381 agcgcggcgt gctcgccagc agcgagcgcg tgtacgggtg cgccggccgg gcgaacacat
     1441 cggctttcga tccgtattcg acgatcttgc ccagatacat caccgcgatc gcatcggcga
     1501 tgtggcgcac gacctggaga ttgtgcgaaa taaaaagata ggcgatgccc atctcctgct
     1561 gcaaatccat catcaggttg agcacctgag cctggatcga gacgtcgagc gccgataccg
     1621 gctcgtcggc gacgaccagc ttcggattga gcatcagcgc acgggcgacc gcgatccgct
     1681 gccgctgccc gccggagaac atgtgcggat agcggcggta atgttcgggc cgcaatccga
     1741 ccttcgccat catcgcccgc gccgcggcgc gacgcgcagc cttgtcgatg ccggcgttga
     1801 tcgccagcgg ctcctcgagc agggttccga ctttcttgcg cggattgagc gaggcgtacg
     1861 ggttctggaa aaccatctgc accatcgggc gcaagcgctt caacgtcgcg ttgtcggcat
     1921 ccacgacgtc gatgccgtcg agcttcaatc cccctgatgt cgggcgctcg atcatcgtga
     1981 cctggcgcgc cagcgtcgat ttgccgcagc ccgattcccc gacgacggca agcgtttccc
     2041 cggcgcgcac ggtgaacgaa acgccatcga gcgcgcgcac gaccgccttg gcgcgaaacg
     2101 ggccgcgcga taccgcgtaa tggcgagtca gccgatcgac ctcgagcagc ggcgcgccgg
     2161 cactcatcgg ctgccggcca tggcgcgcgt tgccgccgcc tcttcggccc agccgtgcgt
     2221 cggccgtccg gcggcatcga gcgcaaaatg gcaacggacc ttgcgtgccg cgggtccggc
     2281 cagcggcggc cggtcgaccc aacagcgcgc cagcgcaaaa tcacagcgcg gcgccaacag
     2341 gcatccggcc ggccgatcgt actggccggg cacgacgccg ggaatcgcct tcagccgcgc
     2401 ccgatcgacg ttgtgctcgg gcagcgcgtc gagcagcgcc ttggtatacg gatgttgcgg
     2461 ggtatcgaag atctgcggga ccggcccggt ctcgacctgc tcgccggcgt acatgacgat
     2521 gacgcgttgc gccgtctcgg cgacgaccgc cagatcgtgg gtgatcagta gcagcgccat
     2581 gcctcgctcc tgctgcagcc gcagcaacag gtcgagcacc tgcgcctgga cggtcacatc
     2641 gagcgcggtc gtcggctcgt cggcgatcag cagcctcgga ttgcaggcga tcgccatcgc
     2701 gatcattacc cgctgcgcca tgccgccgga gagctggtgc ggaaacgcgc ccagccgcgc
     2761 tgcgggatcc gggatctcga cctgctgcag cagttgcagc gcccgtgcgc ggcgcagctt
     2821 ggcactcccg cgctcctcgg gggtgccatg catgcgcagg gtctcggtca gctggaaggc
     2881 gacggtgaaa cagggattca gactcgccag cgggtcctgg aagatcatcg cgatgtcctt
     2941 gccggtcagc gccctgcgct cggctgcgga cagcgacagc agctggcgtc ccgcgaattc
     3001 catgcggcgc gcgcgcacgc gccccggaaa gtcgaccagc cccatcagcg ccagcgcggt
     3061 gacactcttg cccgagcccg attcgccgac gcagccgacg atttcgccgg cgtctatcgt
     3121 cagatcgacg tcatcgaccg ccatcaatgg cgcggcgacg ctgccgaatt cgacggtcag
     3181 accttcgatc tcgagcaaag ccatcgttat cgcttgagct tcgggtcgag cgcatcgcgc
     3241 agaccgtcgc ccatcaggtt gaacgccagc acggtgatca ggatcgcgag gccgggaaag
     3301 gtgacgaccc acgacgcccg ctggatgaat tccagcgcgt cggcgagcat cgatccccac
     3361 tcaggtgtgg gcggctgcgc gcccaggccg agaaagccga gcgcggcggc gtcgaggatc
     3421 gcgttggaaa agccgagtgt tgcctgcacg atcagcggtg cggcgcaatt gggcaggatc
     3481 gtattgaaca tcagccgcaa cgggccggcg ccggcgatac gcgacgaagc aacgtactcc
     3541 ttcgcggctt cggcgatcac cgcggcgcgg gtcagccgca cgtaatgcgg cagcaacacg
     3601 atcgcgatcg catacatggc gttgatcaac cccggcccga ggatggcgac gacggcaatc
     3661 gcgagcagca ggctcggcag cgacagcatg acgtccatca ggcgcatgat cgtcgtctcg
     3721 accagcccgc gaaaatagcc ggcggccagt ccgaggacga cgcccagcga cagcgagatc
     3781 gcaaccgaga tcacgccgat cagcagcgac agccgtgtgc cgtgaatcag ccgcgaaagg
     3841 atgtcgcgtc cgaccgggtc ggttccgagc agaaagcgcg agctgccgcc gtcgtgccat
     3901 gccggcggag ccagcgtgaa ctcgcggaat tgctcatacg gcgaatgcgg cgcgatgacg
     3961 ttggcgccga tcgccagcac gaccagcacg acgatcagcg cgaagccgat gatcgcgcca
     4021 cggctttgcg tgtacgccag ccagaattcc cgcaaggtcc cgggaacggc cggcggcaca
     4081 tcggcggcga tggcggtgac ttcgctcatg cgtggcgtat gcgcggattg atcacgccgt
     4141 agagcaggtc gacgatcagg ttgacactga tcacgatcgt cgcgatcagc aggatgccgc
     4201 cctgcacgac cgggtagtcc cgcgactgca cgccgtgcac cagccagttg ccgacacccg
     4261 gccaggagaa gatcgtctcg gtgaggatcg cccccgaaag cagcaggccg acctgcaggc
     4321 cgatgacggt aatcaccggg atcagcgcat tgcgcagcgc gtgcaccagc acgacctgcc
     4381 ggtacggaat tcccttcgcg cgggccgtgc gcacatagtc ttcgcgcagg acttcgagca
     4441 tcgacgatcg cgtcatccgc gcgatcaccg ccagcgggat cgtgcccagc gcgatcgtcg
     4501 gcagcagcag gtgcgagacc gcggagcgga aggcgccggc atcgccggcc cgcagcgtat
     4561 cgatcagcat gaatccggtt gacgccggca cgtcgaactc gacggcgatc cgtcccgaca
     4621 ccggcgtcca tccgagctgc accgaaaaga acaggatcag cagcagcgcc caccagaata
     4681 tcggcatcga gtagccggtc agcgagacgc ccatcaccgc gtagtcggca acggtgtttc
     4741 gcttcagcgc cgcgatgatg cccgctggca ggccgacggc gaccgcgaac agcatcgcga
     4801 aaaatgcgag ctccaccgtc gccggaaaca aggtcaggaa ttcgcgcagt accggctcgt
     4861 gcgtggtcag cgacgtgccg agatcgccac gcagcacctg gccgatatag tcggcgtatt
     4921 gcaccggcag cggacggtcg aggccgaact cgtgcaacag gcggaggcgc cgcgccgggt
     4981 ccatgccgcg ctcgccggac agattctcga ccggatcgcc gggaatcagg tggatcagcg
     5041 cgaacgccag caacgtcacg ccgaaaaacg tcggaacgat cagcgcggcg cggcgaagga
     5101 tgaaggtcag catcgaaacg tccggctacg ctgcccagac gcgactaaag cgcacgcgcg
     5161 agaagccaac gccggacgcg gcccgtcctg cgcgggccgc gtccggatcg atcagtcact
     5221 gcgcgttgtc gcggacgacg tcggcgttgg tgaaatagtg gtgcgcggtc gcatccatct
     5281 tgtagccttt gacctccttg cggatcgggt cgaaacgcac cgaatgcgca agcgtgatcc
     5341 acggcgcctc ttccttgaat atcacctggg cctgctcgta gaacttggcg cggtcggcct
     5401 tgttcggcgt ctgcgccgcc ttgatcacca agtcctcgaa tggcttgttg caccagcgcg
     5461 cgacgttgcc gccgcccttg accgcttcgc agcccagcaa cggcacgaag aagttgtcgg
     5521 gatcgccgtt gtcgcccgac cagccgaaca tcatcaccgc ctgctcgccg gtcttgctgc
     5581 gcttgcggta ctcggcccat tcgtaggtga cgagcttggc cttgacgccg atcttgtccc
     5641 agtcggcctg gatcatctcc gccatgcgct tgccgtcggg gttatacggc cgcgtcaccg
     5701 gcagatacca aagatccact tcgaagccgt tggggtaccc ggcctgcgcc agcagctgtt
     5761 tcgccttcgc cacgtcgtac gcgtaatcct gcacggcgtt gttgtagctc cacaggatcg
     5821 gcggaatcgg gttcttggcg acctggccct gcccctggta gaccgccttc aggatcgcgt
     5881 ccttgttggt ggccatgttc agtgcctgcc gcaccagctt gttgtcgaaa ggctttttct
     5941 cgacgttgaa cgcgatgtag ccgacgttga ggccttcctt ctgcagcacg ttgagctgcg
     6001 gatccttctg catctcggcc aggtcggcag gcttcgggaa cgccatcgcc tggcactcgc
     6061 cggtcttgag cttggcgtag cgcgcggtcg cgtcgcgggt gatcgagaag atcaggttgt
     6121 cgatcttcgg ccggcccttc cagtactgat cgaacgcctt gtaccgtatc gtcgcgtcct
     6181 tctgatacga cacgtattcg aacgggccgg tgccgatcgg atagatgtcg gcggcgtttg
     6241 gcgttccctt tttcatcatc gtttcgaagt attcggccga gttgatcgag gcgaaatcca
     6301 tcgccatgtc ggcgaggaac ggcgcctccg gccgcttcaa cgtaaagcgc accgtgtact
     6361 tgtcgaccgc ctcgaccttg tcgacgatat tcttcatgcc catgtcgtcg aaatacgcgt
     6421 acgtctggcc ggccgtcatc ttgtggaacg gatgattgtc gtcggccatg cgggtccacg
     6481 aaaaaatcac gtcgtcggcg ttgaaatcgc gcgtcggctt gaagttggcg ttgctgtgga
     6541 acttgacgcc gtgccgcagc ttgaacgtga cgaccttgct gtccggggag acggtccacg
     6601 actcggccag cgccggaatg atgttggtcg tgccggtttc gaattc
//
LOCUS       DATANS010000767         1915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_164452, whole genome shotgun sequence.
ACCESSION   DATANS010000767 DATANS010000000
VERSION     DATANS010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164452"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..>1915
                     /locus_tag="VHT22_11420"
     CDS             237..>1915
                     /locus_tag="VHT22_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HEX3138214.1"
                     /translation="MALTGALFSLHANAYRVEVDAPAPLRKFLEQFLDLARYKDRTDL
                     NDDQFNFMLGDAPQQVKDLAATEGYFSPQTKVEVERSGAEPVVRITVEPGPRTLISTV
                     NLDVSGPAADESPQQIAELRNKWGLPAGNPFRQQDWSEAKENGLRILQNRNYASARIA
                     DSRAAIDSAAHKAELDVEYESGPRYTLGRLNVTGTRRYPATIVHNINPLKEGEAYDVD
                     RLRELQRQSQTTRYFSNATVDIQRDPENPLDSPVNVRVSEFPTQLLRGSAGYSTDDGA
                     RVRGEYSHNNVFGKAWGFDSQASLEQRRQFGSVGLSMPPDSSAFVNNAHASYERTTLS
                     GLDLRSRRIGLKRSRTTQNYDLAWTLEYYSDQLEQISGATVPLDIVAQPGSHQALVAG
                     VRWTRRRLDSLLFPRDGHILTLEGGVAVKGVLTEETFARLLAQGRKYIPVAQRDLVLL
                     RAELGAVITREGSNAAVPGSLLFRAGGTNSIRGYTSQSIGNEVNGATYPTRLLATASA
                     EYQHWFTQDWGGAAFYDAGTATDTWSERSLFNGVGVGVRYRTPVGTINADIA"
BASE COUNT          366 a          633 c          581 g          335 t
ORIGIN      
        1 cgtctaaccc gatctacgac agcacggacc accggtcacg cgcctgttga ctcgtgcagg
       61 tgtcgtgcgt cgcttactcg cgcgaggaga cttgttcatg gccggcgctt tcaatcaccc
      121 gggcttgcga cggctttcgt agccttcacg gtcgactgtc gatgcaaggc cgccgcgctt
      181 tcccgacgcc agcgcgctgg ctcctgcgat accggcaggc accgagcctc gttgcgttgg
      241 cgcttaccgg cgcgcttttt tcccttcatg caaatgccta tcgcgtcgaa gtagacgcgc
      301 ccgcgccgct caggaaattc ctcgagcagt ttctcgatct ggcgcgctat aaggaccgca
      361 cagatctgaa cgacgaccag ttcaatttca tgctcggcga tgccccgcag caggtgaagg
      421 atctcgccgc gaccgagggc tatttttcgc cgcaaacgaa agtcgaggtc gagcgcagcg
      481 gcgccgagcc ggtcgtgcgc ataacggtcg agccaggacc gcgcacgctg atctcgaccg
      541 taaatctcga cgtttccggt cccgccgctg acgagtcgcc gcagcagatc gccgaattac
      601 gcaacaagtg gggtcttccg gccggcaatc cgttccgcca gcaagactgg agcgaagcaa
      661 aggagaacgg gctgcgcatt ctgcaaaatc gcaactacgc ttcggcgcgc attgccgact
      721 cgcgtgcggc gatcgattcc gcagcgcata aagccgagct cgacgtcgaa tacgaaagcg
      781 ggccgcgcta cacgctgggg cgcctcaacg ttacgggcac gcgccgttat ccggcgacga
      841 tcgtgcacaa catcaatccg ctcaaggagg gcgaagccta cgacgtcgat cgcctgcgcg
      901 agctgcagcg tcagagccag acgacgcgtt acttcagcaa tgcgaccgtc gatatccagc
      961 gcgaccccga aaatccgctc gacagccccg tcaacgtgcg cgtcagcgaa tttccgacgc
     1021 aactcctgcg cggttctgca ggttacagca ccgacgacgg cgcgcgcgtg cgcggcgaat
     1081 actcgcacaa caacgtcttc ggcaaagcgt ggggtttcga cagccaggcg agcctcgagc
     1141 agcgtcgtca gttcggctcg gtcggactgt cgatgccgcc cgattccagc gcgttcgtca
     1201 acaatgcgca cgcgtcctac gagcgcacga cattgtcggg cctcgatttg cgcagccgcc
     1261 gcatcgggct caaacgctcg cgcacgacgc agaactatga tctcgcgtgg acgctcgaat
     1321 actatagcga ccagctggaa cagatcagcg gcgcaaccgt gccgctcgac atcgtcgccc
     1381 agcccggctc gcatcaggcg ctggttgcgg gtgtcaggtg gacgcgccgc cggctggata
     1441 gcctactatt tccacgcgat ggccatatcc tcacgctcga aggcggagtc gccgtgaaag
     1501 gcgttttgac cgaagaaact tttgcccggc tgcttgcgca gggccgcaaa tacatcccgg
     1561 tcgcgcagcg cgatctcgtg ctgctgcgcg ccgaattggg cgcggtaatc acgcgcgaag
     1621 gcagtaacgc agcggtgccg ggatcgctgc tgtttcgggc cggcggcacg aattcaattc
     1681 gcggctacac gagccaaagc atcggcaatg aggtcaatgg cgcgacctat ccgacgcgcc
     1741 tgctggcaac cgccagcgcc gaatatcagc actggtttac ccaggactgg ggcggcgccg
     1801 cgttttacga cgcgggcacg gcgaccgaca cgtggtccga acgttcgctg ttcaatggcg
     1861 tcggcgtcgg cgttcgctat cgcactcccg tcggcacgat caacgccgac atcgc
//
LOCUS       DATANS010000768         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_38172, whole genome shotgun sequence.
ACCESSION   DATANS010000768 DATANS010000000
VERSION     DATANS010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38172"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VHT22_11425"
     CDS             <1..472
                     /locus_tag="VHT22_11425"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3138215.1"
                     /translation="QFYRDDLAQRISEIVRNAGCQPSWIELEVTESSLLHDLDAIRKV
                     LQKLRDEGFSVAIDDFGTGYSSLSHLKHFPIDTLKIDISFIADLETDPGDAAITEAIV
                     ALARGLNLRVVAEGVGTSGQLAFLEARDCHCFQGFLFSEAMPPDEFSKFAAKHK"
     gene            complement(523..1323)
                     /locus_tag="VHT22_11430"
     CDS             complement(523..1323)
                     /locus_tag="VHT22_11430"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3138216.1"
                     /translation="MLPPESRLEVRTRLPAKPNAKPPLLFVHGGYCDAWCWEPYFLPY
                     FAAQGYAAHALSLRGHGASGGREALFVTGLDDYVADVEQVAGSLPSPPVLIGHSMGAA
                     VIERMLTTRPIRAAALLAPIPPAGLGPVAQRLLAWQPEYLVNMHRLDAPHLSGDVLAA
                     LRPMYFSASVPPKILAEALRHLTPESPRALMDLTLRLHGGAPVAPARLFVLGANDDRI
                     STPADVKATASLYGVPSLVLPGLAHMLMLERDWETAARPLLAWLKASG"
     gene            1461..2159
                     /locus_tag="VHT22_11435"
     CDS             1461..2159
                     /locus_tag="VHT22_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX3138217.1"
                     /translation="MSRLHRRESDHAAVPAAAYDVDCRRCRRLAQFLDQVLAANPGYF
                     CRPVPPFGDMSARLVIVGLAPGMHGANASGRPFTGDYAGIMLYATLHKFGYANRAQSL
                     SADDGLELIDARITNAVKCLPPGNKPLPEEIRNCNGYLAAELGTLPDGAAILALGRIA
                     HDATLRALGLGSRAYPFAHGAEHALPRGDGGTVQLFDCYHCSRYNTNTRRLTGTMFEQ
                     VFASIARFRADATA"
     gene            2174..>2573
                     /locus_tag="VHT22_11440"
     CDS             2174..>2573
                     /locus_tag="VHT22_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007741088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX3138218.1"
                     /translation="MSDENTSGRKSEAFDARELLKSLPNRAGVYRMVDAAGDTLYVGK
                     ARDLKKRVSNYFQKTEHHPRTEAMVAQVARVEVTVTRSEGEALLLENNLIKAHQPRYN
                     VVFRDDKSYPFISLTGETFPQLRFHRGKLDK"
BASE COUNT          483 a          853 c          812 g          425 t
ORIGIN      
        1 ccagttctat cgcgacgacc tggcgcagcg tatttccgag atcgtgcgca atgccggctg
       61 ccagccgtct tggatagagc tcgaggtcac cgagtcaagc ctgctgcacg acctcgacgc
      121 catccgcaaa gttctgcaga aactgcgcga cgaggggttc tcggtggcga tcgacgattt
      181 cggcaccggc tactccagcc tgtcgcacct gaagcacttc ccgatcgaca cgctgaagat
      241 cgacatttcg ttcatcgccg atctggagac cgacccgggc gatgcggcca tcaccgaggc
      301 gatcgtcgcg ctggcacgcg gccttaattt gcgggtcgtc gccgagggtg tcggcaccag
      361 cggtcagctc gctttcctcg aagcccgtga ctgccactgt ttccagggtt tcctgttcag
      421 cgaagcgatg ccgcccgacg aattctccaa atttgccgcg aagcataaat agtattcgga
      481 ttcaccgcgt cgccgccgtg acagatgatc gtattgcgaa agtcagcctg acgccttgag
      541 ccaggcgagc agcggccgcg ccgcggtttc ccaatctcgc tccagcatca gcatatgcgc
      601 gagccccggt aacaccagcg aaggaacgcc atacagcgac gccgtcgcct tgacgtcggc
      661 cggtgtcgaa attctgtcgt cgttcgcgcc cagcacgaac agccgcgccg gcgcgaccgg
      721 agccccgccg tgcagccgca gcgtcaggtc catcagcgcg cgcggcgatt cgggcgtcag
      781 atggcgaagc gcctcggcca ggatcttcgg cggaacgctg gcgctgaagt acatcgggcg
      841 aagcgccgcc aatacatcgc cgctcaagtg cggcgcatcg agccgatgca tgttgaccag
      901 gtattcgggc tgccaggcga gcaggcgctg cgccaccggc ccaaggcccg ccggaggaat
      961 cggcgccaat agcgccgcgg cgcgaatcgg acgcgtcgtc agcatgcgct cgatgaccgc
     1021 ggcgcccatc gaatgtccga tcagtacggg cggcgagggc aagctgccgg cgacttgctc
     1081 gacatcggcg acatagtcgt cgagaccggt gacgaacagc gcttcgcggc cgccgctcgc
     1141 accatgtcca cggaggctca gcgcatgcgc cgcatatccc tgcgcggcaa agtaggggag
     1201 gaaataaggc tcccagcacc atgcgtcgca atagccgccg tgcacgaaca gcagtggcgg
     1261 cttcgcgttc ggcttggcgg gcagccgcgt acgcacttcg agtcgtgatt cgggaggaag
     1321 catcggagcc ggtaggcacg cgagggcgtg ctaaaattac gacaacccgc cgccatttcc
     1381 ggcgcacgct ccggcgattc cgcggcaatc cgcagcgatc cgcagtgatg atacggcggc
     1441 gggaaaacgc gccattctac atgagtcgat tgcaccgccg cgaatccgat catgccgcgg
     1501 tcccggccgc cgcgtacgac gtcgactgcc gacggtgccg gaggctcgcg cagtttctcg
     1561 atcaggttct cgctgcgaat ccgggctatt tctgccggcc cgtgccgccg ttcggggaca
     1621 tgagcgcgcg cctggtgatc gtcgggctcg ccccgggcat gcatggggcc aacgccagcg
     1681 gcaggccgtt taccggcgac tacgcgggca tcatgctgta cgcgaccttg cacaagttcg
     1741 ggtacgcgaa ccgggcgcag tcgctgagcg cggacgacgg tctcgaattg atcgacgccc
     1801 gcatcaccaa cgcggtcaaa tgcctcccgc cgggcaacaa gccgctgccc gaagagatca
     1861 ggaactgcaa cgggtatctt gctgcggagc tcggcacgtt gcccgacggc gccgcgatcc
     1921 tcgcgctggg acgaatcgcg cacgacgcta cgctgcgggc gctgggtctg ggatcgcgcg
     1981 cgtatccgtt tgcgcatggc gccgagcacg ctctgccgcg gggcgacggc ggcacggtgc
     2041 agcttttcga ctgctatcac tgcagccgct acaacaccaa cacgcggcgc ctgacgggga
     2101 cgatgttcga gcaggtattc gcgtcgattg cccgttttcg tgccgacgcc actgcgtaat
     2161 tgagttgaag ccgatgtccg acgaaaatac gtccggcagg aaaagcgaag ccttcgacgc
     2221 ccgcgagttg ctcaagtcgc tgcccaatcg cgccggcgtt taccgcatgg tcgatgcggc
     2281 tggcgacacg ctatacgtcg ggaaggcgcg cgacctgaaa aaacgcgtct ccaattactt
     2341 ccagaagact gagcaccatc cacgcaccga agcgatggtc gcccaggtcg cgcgcgtcga
     2401 agtgacggtc acgcgttccg aaggcgaagc gctgctgctc gagaacaatc tgatcaaggc
     2461 gcaccagccg cgctacaacg tcgttttccg cgacgacaag agctatccgt tcattagcct
     2521 gaccggcgaa acgttcccgc agctccgctt ccaccgcggc aagctcgaca agc
//
LOCUS       DATANS010000769         2410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_542711, whole genome shotgun sequence.
ACCESSION   DATANS010000769 DATANS010000000
VERSION     DATANS010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542711"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..648
                     /gene="mtgA"
                     /locus_tag="VHT22_11445"
     CDS             10..648
                     /gene="mtgA"
                     /locus_tag="VHT22_11445"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEX3138219.1"
                     /translation="MLLQLWYAAHIWWWRDHPPSETAFMSVRLAELKRVNPGARLQYT
                     WVPYQRISVQLKRAMIAAEDAKFVEHEGFDWEGIQHAMDKNLKKGRVVAGGSTISQQL
                     AKNLFLSGDRSYWRKGEEALITVMLEAILDKRRIFEIYLNVIEWGTGVFGSEAAARHY
                     FGTSAAQLDAEQAARLAAMTPNPRRYERNPGSKVLARRIPIIMAGMPSAELP"
     gene            789..1172
                     /locus_tag="VHT22_11450"
     CDS             789..1172
                     /locus_tag="VHT22_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138220.1"
                     /translation="MNTTVRKIGTIAASLFAALALSAVALAQAPTKMAGGVMADSAGM
                     TLYVFDKDAGGKSACNGPCAAIWPPLLVTGDGKASGDWTIVARDDGTKQWAFKGKPVY
                     KYSKDTKAGETTGDGFNGVWHAVKG"
     gene            1345..1872
                     /locus_tag="VHT22_11455"
     CDS             1345..1872
                     /locus_tag="VHT22_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX3138221.1"
                     /translation="MPDSLPHPDLVAALPRLRRYARVLTGDLNRADDLVQDTLARAWE
                     KRRLWQPGSDLRAWLFTIMHNVHVNQFAVRRREQSESSLDADDGPVAGWEIPVRATQS
                     DRVELAEVFAQMGRLPVEQREVLILAAVEELRYEEIARVQCVPIGTVMSRLNRAREKL
                     RRMCEVSASPLKVIK"
     gene            1869..>2410
                     /locus_tag="VHT22_11460"
     CDS             1869..>2410
                     /locus_tag="VHT22_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX3138222.1"
                     /translation="MSSNLPLSDDDVHAYADGKLSGERRARLAAALERDPALAARVAA
                     IRRQNVDLREAFDPWLAEPLPRELIAGAQRPRGRKPPRGRWAMPALASAATLIIGIGL
                     GWMGRGEVLHLAGTPTTFPRQAALTHALYATDANRPVEIWANEEDRLVRWLSKRLNFP
                     LHAPNLHAMGYDLVGGRLVAG"
BASE COUNT          436 a          735 c          842 g          397 t
ORIGIN      
        1 accgctttcg tgctgctgca gctgtggtac gcggctcaca tctggtggtg gcgcgatcat
       61 ccgcctagtg aaacggcttt catgtcggtc cgcctggccg agctgaagag agtcaaccct
      121 ggcgcgcgcc tgcaatatac gtgggtgccc tatcagcgca tttcggtcca gctgaagcgc
      181 gcgatgattg ccgcggaaga tgcgaaattc gtcgagcacg aagggttcga ctgggaaggc
      241 atccagcacg cgatggacaa gaacctgaaa aagggccgcg tggtcgccgg aggatcgacg
      301 atttcgcagc agctggcgaa gaaccttttc ctttcgggag atcgaagcta ttggcgcaag
      361 ggggaagagg cgctgatcac cgtgatgctc gaggcgattc tggacaagcg tcgcatcttc
      421 gagatctacc tgaacgtcat cgaatggggc accggcgtgt tcggctccga ggccgccgcg
      481 cgtcactatt tcggcacatc ggcggctcag ctcgacgccg aacaggcggc acggcttgcc
      541 gcgatgacgc cgaacccgcg ccgatacgaa cgcaatccgg gatcgaaagt tttggcgcgc
      601 aggatcccca tcatcatggc gggaatgccg tcggcggagt tgccctgagg cgcattgtgg
      661 ggcacgatgc gcggcacgcc gcccgggcga gccgcgcccg gcatcatggg aataagatct
      721 ggcggcgcgt gttagcggtg ccgtagctcg accgaagcat tgacgatatt aaccaacggg
      781 gagaagtgat gaatacgacc gtacgcaaaa tcggcacaat tgcagcaagc cttttcgccg
      841 cgctcgcatt atccgctgtc gcgctggcac aagccccgac caagatggcc ggcggcgtga
      901 tggccgacag cgcgggcatg acgctctatg tgttcgacaa ggatgccggc ggcaagagcg
      961 cctgcaacgg tccgtgcgcc gcgatctggc cgccgctgct ggtcaccggc gatggcaagg
     1021 cgtcgggcga ctggacgatc gtcgcgcgcg acgacggcac caagcagtgg gcgttcaagg
     1081 gcaagccggt ctataagtat tcgaaggata cgaaggcggg cgaaacgacc ggcgacgggt
     1141 tcaacggcgt ctggcacgcc gtaaagggtt gaccaggagc ggatgcgccg cgatgtccgg
     1201 cgcatccgac cgaggcccgc gacagggaat acccccggcc gtcctgctgt tcccttcacc
     1261 atgaagccgg cggcaaagca cgtggcaggg gccgccggga ataacgcggc gcccgcagcg
     1321 gtctgacgta cagggaaccg tcggatgccg gactcgttgc ctcaccccga tctggttgcg
     1381 gcgcttccgc gcctgcggcg ctacgcgcgg gtgctgaccg gcgatctcaa tcgcgccgac
     1441 gatctggtgc aggacacgct ggcgcgcgca tgggagaagc ggcggctatg gcaacccggt
     1501 agcgatctgc gagcctggct gttcacgatc atgcacaacg ttcacgtcaa ccagtttgcc
     1561 gtccgccgtc gcgagcagtc ggaaagctcg ctggacgccg acgacggccc ggtcgccggc
     1621 tgggaaattc cggtgcgcgc cacccagtcc gatcgggtcg agctcgccga agttttcgcg
     1681 caaatgggac ggcttccggt cgaacagcgc gaagtgctga ttctggctgc ggtggaggag
     1741 ttgcgctacg aagagatcgc gcgcgtgcag tgcgttccga tcggtaccgt gatgtcgcgt
     1801 ctgaatcgcg cccgggaaaa gttgcggcgc atgtgtgaag tctcggcctc gccgctgaaa
     1861 gtgatcaaat gagctcgaac ctgccattgt ccgacgacga cgtgcatgcg tacgccgacg
     1921 ggaagctatc gggggagcgc agggccaggc tcgcagctgc gctcgagcgt gacccggcgc
     1981 tcgcggcgcg ggtcgccgcg atccgcaggc agaatgtcga cctgcgcgag gcgttcgatc
     2041 cctggctggc cgagccgctg ccgcgcgagc tgatcgcggg ggcgcagcgg ccgcgtggca
     2101 ggaaaccgcc gcgcgggcgc tgggcgatgc cggcgttggc gagcgccgcc acgttgatca
     2161 tcggcatcgg gctcggatgg atgggccgcg gcgaagtcct gcatcttgcc ggcacgccga
     2221 cgacgttccc gcgacaggcc gcgctgaccc acgcgcttta tgctactgac gccaatcgtc
     2281 cggtcgagat ctgggccaac gaagaggacc gcctggtgcg gtggctttcg aagcgtctga
     2341 attttccgct gcacgcgccg aacctgcatg cgatgggtta cgacctggtc ggggggaggc
     2401 tggtcgcagg
//
LOCUS       DATANS010000770         3605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_375080, whole genome shotgun sequence.
ACCESSION   DATANS010000770 DATANS010000000
VERSION     DATANS010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375080"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VHT22_11465"
     CDS             <1..558
                     /locus_tag="VHT22_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX3138223.1"
                     /translation="GMRRGELTHMESDSRGRTRLEYRVPARGLIGFQGEFMNLTRGTG
                     LISHVFDGYAKVGGDIPERRNGVLISAEAGDAVAYALWKLQERGRMFVSPGDKLYEGM
                     VIGIHSRDNDLVVNPIKGKQLTNVRASGKDEAIALTPPIQLTLEYAVEFIADDELAEV
                     TPKSIRVRKRHLLAHERKRASREAA"
     gene            631..2019
                     /gene="rmuC"
                     /locus_tag="VHT22_11470"
     CDS             631..2019
                     /gene="rmuC"
                     /locus_tag="VHT22_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX3138224.1"
                     /translation="MESTIWLIVSGVGFVVLAALLAVILIRVQALFRIASGVEFKARL
                     DAITAQNERLERELRTELARARGEAAQSAQTGRAELASSLNQFTQSLQNQLAGSAHVQ
                     NERLASLTQSNESRLEAVRATVEQRLEVLRADNAQKLEHIRLTVDEKLHATLEQRLGE
                     SFKLVSDRLEQVHKGLGEMQTLAAGVGDLKKVLTNIKTRGTWGEVQLENLLEQMLTPA
                     QYGKNVVTRPGQSWRVEFAIRLPGRDEGNSEFWLPIDAKFPLEDFQRLQDAQERADLE
                     GVELYGKALEDRVRKQARDIHEKYIEPPHTTDFALLYLPVESLYAEVLRRPGLSDLIQ
                     REYQVTLAGPTTLAAILNSLQMGFRTLAIERRSGEVWRLLAAVKTEFGTFGDILAKTK
                     KQLEMVGNTLGAAEIRSRSIERKLRGVEALPESEATLLLREQTAIVIDIETGTSADPE
                     LDLEPPASNSAS"
     gene            2040..2870
                     /locus_tag="VHT22_11475"
     CDS             2040..2870
                     /locus_tag="VHT22_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HEX3138225.1"
                     /translation="MFEAIRNWRRRRTLATAAIPETLWRQALDELPFLAIYTEDEIRR
                     LRERVVVFLSEKGIVGAAAFAVTPLMRVLIAMQACAPALWLDADCYDGWENVIVYPDE
                     FIIAREFEDEAGVVHRHREQLAGESMPGGPVVLSWSDVEAGADWESAGMNLVIHEFAH
                     KLDMRNGDANGCPPLPPDMRPNDWQRTMKIAYEHFRARVESEEDTAIDPYAAESPAEF
                     FAVLSEVFFAEPALLLHEYADVYDLLRRYYRQDPARRSELLMDWDHVLPGDGPAPSAV
                     "
     gene            complement(2923..>3605)
                     /locus_tag="VHT22_11480"
     CDS             complement(2923..>3605)
                     /locus_tag="VHT22_11480"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3138226.1"
                     /translation="LHHAQHRRAFGHPLAEQPLMQNVLADLAIEAEAAAASALRLAST
                     FEANADEAEAALSRLATPALKYWLCKRAPMVVAEAIEVLGGNAYVEESALPRLYREAP
                     LNSIWEGSGNIMCLDVLRAARREPNAVAALLDELALASGGDARLDRHIARLGATFSDG
                     ALDEAGARRLAADVALALAAALLVRNAAPAVADAFCASRFGESYSGALGTLPSGVDCA
                     GVLTQAGG"
BASE COUNT          669 a         1153 c         1208 g          575 t
ORIGIN      
        1 ggcatgcggc gcggcgagct gacgcacatg gaatcggaca gccgcggacg cacgcgactc
       61 gagtaccgtg ttccggcgcg aggcctgatc ggcttccagg gcgagttcat gaacctcact
      121 cgcggcaccg gcctgatcag ccacgtcttc gacggctatg ccaaggtcgg cggcgacatt
      181 ccggagcggc gaaacggcgt gctgatctcg gcggaagccg gcgatgcagt cgcctacgcg
      241 ctgtggaagc tgcaggagcg cgggcgcatg ttcgtctctc ccggcgacaa gctgtacgaa
      301 ggcatggtga tcggcatcca cagccgcgac aacgacctgg tcgtcaatcc gatcaagggc
      361 aagcagctga ccaacgtccg cgcgtccggc aaggacgaag cgatcgcgct gactccgcct
      421 atccagctga cgctcgaata cgcggtcgag ttcatcgccg acgacgagct cgccgaggtc
      481 acacccaaat cgattcgagt ccgcaagcga catctgctgg cgcacgagcg caagcgcgca
      541 agccgcgaag ctgcctgagc cgccgcatcg gaacgcggcg cgaacagact cgctttgccg
      601 cgctgaggcc ggcgacgtaa actgtcggac atggaatcga ccatctggct gatcgtctcg
      661 ggagtgggct tcgtcgtgct ggcagcgctg ctcgccgtta tcctgatccg cgttcaggcg
      721 ctgtttcgga tcgcgagcgg cgtcgaattc aaagcccgtc tcgacgcaat caccgcgcaa
      781 aacgagcgcc tggaacggga gcttcgcacg gagcttgcac gggcgcgcgg tgaggcggca
      841 cagagtgcgc agaccgggcg agccgagctc gcctccagcc tcaaccagtt cacacagtcg
      901 ctgcagaacc agcttgccgg cagtgcgcac gtccagaacg agcgcttggc gtcgctgacg
      961 cagtcgaacg agtcgcgcct cgaggccgtc cgggcgacag tcgagcagcg gctggaggtt
     1021 ttgcgcgcgg acaacgcgca aaagctcgag catatccggt tgacggtcga cgaaaagttg
     1081 cacgcgaccc tcgagcaacg cctgggcgaa tcgttcaagc tcgtgtccga ccgtctcgag
     1141 caggttcaca agggactggg cgagatgcag acgcttgcgg ccggcgtcgg cgatctgaag
     1201 aaggttctga ccaacatcaa gacgcgcggg acctggggcg aagttcagct ggaaaatctg
     1261 ctcgagcaga tgctgacacc ggcgcagtac ggcaagaacg tcgtcactcg acccggtcag
     1321 tcctggcgcg tcgaattcgc gattcgcctg ccgggccgcg acgaaggcaa cagcgagttc
     1381 tggctgccga tcgacgccaa gtttccgctc gaggattttc agcgcctgca ggatgcacag
     1441 gaaagggccg atctggaggg tgtggagctt tacggaaagg cgctggagga tcgcgttcgc
     1501 aagcaggcca gggacattca cgagaaatac atcgagccac cgcatacgac ggactttgcg
     1561 ttgctgtatc taccggtaga aagcctgtat gcggaagttc tgcggcgtcc gggcctcagc
     1621 gacctcatac agcgcgagta tcaggtcacc cttgccggcc cgacgacatt ggccgcaatc
     1681 ctgaatagtc tgcagatggg ctttcggacg ctcgccatcg agcgccgttc cggcgaggta
     1741 tggcggttgc tcgctgccgt gaagaccgag ttcggaacgt tcggcgacat tctcgccaag
     1801 acgaagaagc aactcgagat ggtcggcaac acgctgggcg ccgcggagat acggtcgcgc
     1861 tcgatcgagc gcaagctgcg gggagtggag gcattgccgg aaagcgaggc gacgctcctt
     1921 ttgcgggaac aaaccgcgat cgtgatcgac atcgagaccg ggaccagcgc ggatccggag
     1981 ctcgatctcg aaccgccggc gtcgaatagc gcgagttgac gttcgcgtcg gtgtaggcga
     2041 tgttcgaagc gatccgcaac tggcgtcgtc ggcggacgct ggcgacagcg gcaatccccg
     2101 agacgctgtg gcggcaggcg ctggacgagc tgccatttct ggcgatctac accgaagacg
     2161 agatcaggcg cctgcgcgag cgcgtcgtcg tgttcctctc ggaaaaagga atcgtcggcg
     2221 ccgccgcctt cgccgtaacc ccgctgatgc gcgtcctgat cgcgatgcag gcctgtgccc
     2281 cggcactctg gctcgatgcg gattgctacg acggctggga aaacgtcatc gtctatccgg
     2341 acgaattcat catcgcgcgc gagttcgagg acgaagccgg cgtcgtgcat cggcaccggg
     2401 agcagctggc cggcgagtcg atgcccggcg ggccggtcgt gctgtcctgg tccgacgtcg
     2461 aagcgggcgc cgactgggaa agcgccggga tgaacctagt cattcatgaa ttcgcgcata
     2521 agctcgacat gcgtaacggc gacgccaacg gctgcccgcc gctgccgccc gatatgcggc
     2581 caaacgattg gcagcggacg atgaagatcg cgtacgagca cttccgcgcg cgcgtcgagt
     2641 ccgaagaaga caccgcgatc gatccgtacg ccgcggagag cccggcggaa ttctttgccg
     2701 tcctgtccga agttttcttt gccgaaccgg cgctgctgct gcacgagtat gccgatgtct
     2761 acgatttact gcgtcgatac taccggcagg acccggcgcg ccggtccgag ctgctgatgg
     2821 actgggacca cgtgctgccg ggggacggcc cggcgccaag cgcggtgtaa atagtgctcc
     2881 ggccggccgc gacggatgcg gtcacccgca tcggctcgtt tgtcagccgc cggcctgggt
     2941 gaggacgccg gcgcaatcaa cgcccgacgg cagcgttccc agcgcacctg aatagctttc
     3001 gccgaagcga gatgcgcaga acgcatcggc gaccgccgga gcggcgtttc tcaccagcaa
     3061 cgccgcagcc agcgccagcg caacatcggc ggcgagccgg cgcgctcccg cctcatcgag
     3121 cgcgccgtcg ctgaacgtcg cgccgagccg agcgatgtgc cggtcgagac gcgcatcgcc
     3181 tccgctcgcg agtgcgagct cgtccagcag cgccgccacc gcgttcggct cgcgccgcgc
     3241 ggcgcgcagg acatcgagac acatgatgtt gcccgagcct tcccagatcg agttcagcgg
     3301 cgcctcgcgg tacaggcgcg gcaacgcgct ttcctcgacg taggcattgc cgcccagcac
     3361 ttcgatcgcc tcggcgacga ccatcggcgc gcgcttgcat agccagtatt tgagtgccgg
     3421 agtcgcgagc cgcgacagcg ccgcctccgc ctcgtctgcg tttgcctcga acgtcgaggc
     3481 cagccgcagg gccgatgctg cggcggcttc ggcctcgatc gcgaggtcgg cgagaacgtt
     3541 ctgcatcagc ggctgctcgg ccagaggatg gccaaacgcg cggcggtgct gcgcatggtg
     3601 gagcg
//
LOCUS       DATANS010000771         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_542863, whole genome shotgun sequence.
ACCESSION   DATANS010000771 DATANS010000000
VERSION     DATANS010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542863"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            513..1511
                     /gene="gcvA"
                     /locus_tag="VHT22_11485"
     CDS             513..1511
                     /gene="gcvA"
                     /locus_tag="VHT22_11485"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HEX3138227.1"
                     /translation="MNKTAASKPRARPRLPSLDLLKGFEAAARQLSFTRAAEELFLTQ
                     SALSRQMQTLEEQIGFALFERRHRELRLTDAGQILQATARNVLDELAQAVAKIRREQK
                     SLPLTVSTNQPFASVWLIPRLARFRARHPAIDVFISADNRIVDLERERIDLAVRYCSD
                     AMAPPGAVRLFGERLLPVCSPGLAVDRARPLKRPEDLARHVLLHIDDERGRFPWLNWS
                     VWLAANGIDEIESAGSLRFNHFDQVMQAALDGQGVALGRVPLIDSLLAQGRLVAPFPN
                     RYATTRSYFIVRGTISALRPEAQAFIDWLIEEAAAESALDVSGEPKPARKPRRTKR"
     gene            1508..>1713
                     /locus_tag="VHT22_11490"
     CDS             1508..>1713
                     /locus_tag="VHT22_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3138228.1"
                     /translation="MTDAAIHAAPSPWITRFAPLIPPGARVLDLACGQGRHSRFFAAR
                     LCDIVAVDRDSQALVALAGVPRVTT"
BASE COUNT          262 a          586 c          567 g          298 t
ORIGIN      
        1 aacagcacga tgtcccgcgg atcgcgctcc atcgtcaccc acaggcaccc ggtatcgacc
       61 gtgatcgtcg ttccttcggc gttgttcaat atcagcgact cgcgctgcgg caagtacgcg
      121 cggcggccgc gttggggggt aggcggatcg aatggcattg ctatctcctg gttcatgcgt
      181 aatattcatg catcggccgg tcgctcccag ctgctgcgtt tccggtctct gcagctgaag
      241 tatcggcgcc cggcgagggc ttgaccaacg aattcgccgc atgcgtcgca tgaccaaatg
      301 gaatgcgaac ctgcgcgatg ccgtacggcc attcgcagca tcggcgacag ctggcctaag
      361 cgggcccggc gccgcggcgc cgtgatgcat aatgcgcatg cattcggcgc cgccgggacg
      421 tcgatcggaa tcctgcgtcc gaagcgcggc ggcgcacggc ggcgcgtgcc gcacgccggc
      481 gtccccggcc agcgtctgca tgaaggttca gcatgaacaa gaccgcagca tcgaagccac
      541 gtgcgcgccc gcgcctgccg tcgctcgacc tcctcaaggg gttcgaggcg gcggcgcgcc
      601 agctttcgtt cacgcgcgct gctgaggagc tgtttctgac gcagtcggcg ctgtcccgcc
      661 agatgcagac cctcgaagag caaataggct tcgcactgtt cgagcgccgg caccgcgagc
      721 tgcgcctgac cgacgccggc cagatcctgc aggcaaccgc ccggaacgtg ctcgacgagc
      781 tggcgcaggc ggtggccaag attcgccgcg agcagaaatc tctgccgctg accgtttcga
      841 ccaaccagcc gttcgcctcg gtatggctga tcccgcgact tgcgcgcttt cgtgcccgcc
      901 atccggccat cgacgtgttc atctccgccg acaaccgcat cgtcgatctg gagcgcgagc
      961 gcatcgatct cgccgtacgc tattgcagcg atgcgatggc gccgcccggc gcggtgcggc
     1021 tgttcggcga gcggctgttg ccggtgtgca gcccggggct cgccgtcgac cgcgcgcgcc
     1081 cgctcaagcg ccccgaggac ctggcgcggc acgtcctgtt gcatatcgac gacgaacggg
     1141 gccgctttcc gtggctcaac tggtcggtgt ggctggcggc caacggcatc gatgaaatcg
     1201 agtcggcggg gtcgctgcgc ttcaatcatt tcgatcaggt gatgcaggcc gcgctggatg
     1261 gccagggggt ggcgctgggc cgcgtgccgc tgatcgacag cctgctcgcg caaggcaggc
     1321 tggtggcgcc gtttcccaac cgctatgcga cgacacgctc ctatttcatt gtccgtggca
     1381 cgatttccgc attgcgtccg gaggcgcagg cgttcatcga ctggctgatc gaggaggctg
     1441 cggccgaaag cgcgctcgac gtcagcggcg agcccaagcc ggcacgcaag ccgcgtcgga
     1501 ccaagcgatg accgatgcag cgattcacgc tgcgccatcg ccctggatca cgcgctttgc
     1561 gccgctgatc ccgccggggg cgcgcgtgct cgatctcgct tgcggacagg gccggcacag
     1621 ccgattcttt gccgcccgcc tctgcgacat cgtcgccgtc gatcgcgaca gccaggcgct
     1681 tgtcgcgctc gccggtgtgc cgcgagtaac gac
//
LOCUS       DATANS010000772         1950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_208655, whole genome shotgun sequence.
ACCESSION   DATANS010000772 DATANS010000000
VERSION     DATANS010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208655"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="VHT22_11495"
     CDS             <1..1306
                     /locus_tag="VHT22_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEX3138229.1"
                     /translation="TEYAAEDADITLQLHQALYPQVESDAKLARIYGEIEMPVMPVLH
                     AMERNGVLLDTALLDAQSSELGVKMMELEAAAHQQADQPFNLNSPKQIQEILFGKLGI
                     KPVKKTPSGTPSTDEDALAQLALDHPLPKLLLEYRGIAKLKSTYTDKLPRMVNAQTGR
                     VHTNYAQAVAITGRLSSNDPNLQNIPVRTAEGRRIREAFIAPAGSHIVSADYSQVELR
                     IMAHISQDASLLKAFAAGEDIHRATAAEIFSLEPQAVDGEQRRYAKVINFGLIYGMSV
                     YGLSSQLGIERAAAQQYMDRYFARYPGVAEYMRQTREKAREQGYVETVLGRRLWLPEI
                     RSSNGARRQGAERAAINAPMQGTAADLIKLAMIAVQRWLAADGLKAMLIMQVHDELVL
                     EVPDAEIEVVKTRLPQLMAGVAQLAVPLVVDVGVGPNWEKAH"
     gene            complement(1365..1739)
                     /locus_tag="VHT22_11500"
     CDS             complement(1365..1739)
                     /locus_tag="VHT22_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138230.1"
                     /translation="MPRFYVGLTVDEKEVWSASNVAAEGNLLVSVELSFAGQDAKPLE
                     RYKAALLALGKDATKRRQLSKYCWLFALDAGLNDVFQTLVNHLGVEHHSYSAADLKTG
                     EGLSYDAEKRTVCEFYSKNWMD"
BASE COUNT          374 a          602 c          631 g          343 t
ORIGIN      
        1 aaccgagtat gcggccgagg atgccgatat cacgctgcaa cttcatcagg cgctgtaccc
       61 gcaggtcgaa agcgacgcga agctcgcgcg catctacggc gagatcgaga tgccggtgat
      121 gccggtgctg cacgcaatgg agcgaaacgg cgtgctgctc gataccgcgc tgctcgatgc
      181 gcaaagcagc gagctcggcg tgaagatgat ggaactcgag gcggcggcgc atcagcaggc
      241 ggaccagccg ttcaacctga attcgccgaa gcagatccag gagatattgt tcggcaagct
      301 cggcatcaag cccgtcaaga aaacgccgtc gggcacgccc tccaccgacg aagacgcgct
      361 cgcgcaattg gcgctggacc acccgctgcc caagctgctg ctcgaatacc gcggcatcgc
      421 gaaactcaag tcgacgtaca cggacaagct gccgcgcatg gtgaacgcgc agaccgggcg
      481 cgtgcatacc aattacgcgc aggccgtcgc gatcacggga cgcttgtcga gcaacgatcc
      541 caatctgcag aacatcccgg tgcgcaccgc cgagggccgc cgcatccgcg aggcgttcat
      601 tgcgccggcc ggcagccata tcgtgtcggc ggactattcg caggtggagt tgcggatcat
      661 ggcgcacata tcgcaggacg cgagcctgct caaggcattc gcggcgggcg aggacatcca
      721 ccgcgcgacc gcggcggaaa ttttcagtct cgagccgcag gcggtcgacg gcgagcagcg
      781 gcgttacgcc aaagtgatca atttcggcct gatctacggt atgtcggtat acggcctgtc
      841 gagccagctc ggcatcgagc gcgcggctgc gcagcaatac atggaccgtt atttcgcgcg
      901 gtatccgggc gtcgccgaat acatgcgcca gacccgcgag aaagcgcgcg agcagggcta
      961 cgtcgaaacc gtattgggcc gtcgcctgtg gctgccggaa atccgctcga gcaacggcgc
     1021 gcgccggcag ggcgccgagc gtgccgcgat taacgcgccg atgcagggca cggccgccga
     1081 cctgatcaag ctcgcgatga tcgccgtcca gcgctggctt gccgctgacg ggttaaaagc
     1141 gatgctcatc atgcaggtgc acgacgagct ggtgctcgaa gtgccggatg ccgaaatcga
     1201 agtcgtcaaa acgaggcttc cgcagttaat ggcgggcgtt gcgcagctgg cggtgccgct
     1261 ggtggtggac gtcggcgtcg ggcccaactg ggaaaaagcg cactgagcac ctgccttgcg
     1321 cccgctgcgc gattcatgcg ggcgggcttg agaccgccaa gcggtcagtc catccagttc
     1381 ttcgaataga actcgcacac ggtgcgtttt tccgcgtcat aggacaaacc ctcgccggtt
     1441 ttcagatccg ccgcggaata cgagtgatgc tcgacgccca aatggttgac gagcgtctgg
     1501 aacacgtcgt tgagccccgc gtcgagcgcg aacagccagc agtatttgga aagctgccgc
     1561 cttttggtcg catccttgcc gagagcgagc agtgccgctt tatagcgctc gagcggtttc
     1621 gcgtcctggc cggcgaacga gagctcgaca ctcacgagca ggttgccttc ggcggcgacg
     1681 ttagacgctg accacacttc tttctcgtct accgtgagac cgacgtaaaa acgtggcatg
     1741 cggctctctt tttttctgcg gcagcgctat ttcagtgcgc cgcggatttt ttgtccgatg
     1801 ccgctgccgg cgcggcgggc tcacggcgga gcgccttttc ctcgtcggcg attatggtgt
     1861 atcggccgtg tacaagtttc acgggctcac cggaaattga gcgcgcagac cggcttcagg
     1921 cgataccggc gtcgtgcgcc tgctggtcgg
//
LOCUS       DATANS010000773         2375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_122056, whole genome shotgun sequence.
ACCESSION   DATANS010000773 DATANS010000000
VERSION     DATANS010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122056"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VHT22_11505"
     CDS             <1..803
                     /locus_tag="VHT22_11505"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX3138231.1"
                     /translation="VAGGLIPAAIAPTLAASAAGLQSDVKVDGDDDSWGWNVGVLWQA
                     TDQTRYGFAYRSKIKYDVRGSVNIENPAALGPLPPTLAPVGSAVMAGVNAVLASGDVK
                     LDIEMPDTANVSVFHQFNNKWDLMADIQYTGWSTIQQLQIVRSTGAILSTTPENFRDA
                     WRFSVGANYRYSDVWTWRGGVAFDQSPVRDAERTPRLPDNDRTWIAIGAQYKYSPSIA
                     LDLAWAYIFVKDSNINQNAGSTAANGLISGSYKNDVNIVGLQLTYTFK"
     gene            924..1481
                     /locus_tag="VHT22_11510"
     CDS             924..1481
                     /locus_tag="VHT22_11510"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HEX3138232.1"
                     /translation="MGKRVVLVAAALLSGALSAADIGGIRVEDKASVGGQELVLNGAG
                     LRKKVVFHVYVASLYLPQKATDPAGVLAKGPRRIRLDMLRTLSADALLEALNEGLEAN
                     NSATEMAAIKPGNAQLASIMKTFGQVKEKDVVTLDFYDGATQVGLNGEVKGAVAGEAF
                     NQALTRIWLGDKPVQADLKKALLGG"
     gene            1588..>2375
                     /locus_tag="VHT22_11515"
     CDS             1588..>2375
                     /locus_tag="VHT22_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6352 family protein"
                     /protein_id="HEX3138233.1"
                     /translation="MRVSDDFLRGYLLRPELAPVRESCRAEIAVHEMLLSDPRTRVTE
                     SDVAAIVDPDARDNLRIWLKFRDRLLDAQTIEAAYVGLFEGEGVDVPPLFVHQLTQIL
                     LRHILGADADPIEARAAEMLFRVQKISVQEDAVMAADAQAVELLATTGSLGSLGELLA
                     QNRTPARTVDLDVLSEDNADTYWTRDERFDLAISLNRGQPALAALCRVLQRWIAHFLA
                     VDVAIAPEREIVDTRWVWHVGLDAEASALLNDLYNGVDVADERR"
BASE COUNT          424 a          757 c          796 g          398 t
ORIGIN      
        1 gcgtggccgg cggcctcatc ccggccgcga tcgcgccaac cctcgcggct tcggccgcag
       61 gcctgcaatc cgacgtgaag gtcgacggcg acgacgacag ctggggctgg aacgtcggcg
      121 tgctgtggca ggcaacggac cagacccggt acggcttcgc ctaccgatcg aagatcaaat
      181 acgacgtccg cggctcggtc aatatcgaaa atccggcggc gctcggtccg ctgccgccga
      241 ccctcgcccc ggtaggatcg gccgtgatgg ccggcgtgaa cgccgtgctg gcgagcggcg
      301 acgtcaagct cgacatcgag atgcccgaca ccgccaacgt ttcggtattc catcagttca
      361 acaacaagtg ggacctgatg gcggacatcc agtacaccgg ctggagcacc atccagcagc
      421 tgcagatcgt gcgcagcacc ggcgcgatcc tgtcgacgac acccgagaat ttccgcgacg
      481 cgtggcggtt ttcggtcggc gcgaactatc gctactccga tgtctggaca tggcgcggcg
      541 gggttgcctt tgaccagtct ccggtgcggg acgccgagcg gacaccgcgg ctcccggaca
      601 acgaccgtac gtggatcgcg attggtgctc agtacaaata ctcgccttcc atcgcgctgg
      661 atttagcgtg ggcgtacatt ttcgtcaaag attccaacat caatcagaat gcgggaagca
      721 cggcggccaa cggtctgatc agcggctcgt acaagaacga cgtcaacatc gtcggcctgc
      781 agctgacata cacgttcaag tagcgggcga ccgggaaccc gaccgccgga cgcgtgcggc
      841 ctggcgtcgc ttcggccagg cgagcccgcg ggaatgcggt cgactcggct gccgcatcgc
      901 gttgcatacc ggggagtcct gccatgggca agagggttgt tctggtcgcc gccgcgctgc
      961 tgtccggcgc attgtccgct gccgacatcg gcggcatccg tgtcgaggac aaggcgtcgg
     1021 tcggagggca ggagctggtg ctcaacggcg ccggcctgcg caagaaagtg gtattccacg
     1081 tctatgtcgc cagcctctat ctgccgcaga aagccaccga cccggccggc gtgctcgcca
     1141 agggtccgcg tcggatccgg ctcgacatgc tgcggactct gtccgccgat gcgttgctcg
     1201 aagccctgaa cgagggcctc gaggccaaca acagcgcgac cgagatggcg gcgatcaagc
     1261 cgggcaacgc gcagctcgct tcgatcatga agaccttcgg gcaggtgaaa gaaaaagatg
     1321 tcgtcacgct ggacttttac gatggagcca cgcaggtcgg cctgaacggt gaggtcaagg
     1381 gcgcggtcgc gggcgaagcg ttcaatcagg cgcttacccg gatctggctc ggcgacaagc
     1441 cggttcaggc cgatctgaaa aaggcgctgc tcgggggctg aacaagccgt gccggccgcc
     1501 ggcacggccg catgctatcg tccgctcgtg cccgatttct ggccctcctg cgggtaccgt
     1561 ctgctcgccg tcgacgacga tcggcgattg cgcgttagcg acgacttcct gcgcggctat
     1621 ctgttgcggc cggagctcgc gccggtgcgc gagtcgtgcc gcgccgagat cgccgtgcac
     1681 gagatgctgc tgtccgatcc gcggacgcgg gtgaccgaaa gcgatgtcgc cgccatcgtc
     1741 gatcccgacg cgcgcgacaa tctgcggata tggctcaagt ttcgcgatcg cctgctggat
     1801 gcgcagacca tcgaggctgc ctatgtaggt ctattcgaag gcgaaggcgt cgacgtgccg
     1861 ccgttattcg tccatcagct gacgcaaatc ctgctgcggc atatcctcgg cgcggacgca
     1921 gatccgatcg aggcgcgtgc agcggaaatg ctgtttcgtg tccagaaaat aagtgtgcag
     1981 gaagacgcgg tgatggcggc cgacgcgcag gcggtcgagc tgctggcgac gaccggaagc
     2041 ctgggcagcc tcggcgagtt gctggcacag aaccgcacgc cggcaagaac tgtcgatctc
     2101 gacgtgctgt cggaagacaa tgccgacacc tactggaccc gtgacgagcg gtttgacctg
     2161 gcgatcagcc tcaatcgcgg ccagcccgcg ctcgccgcgc tgtgccgcgt gctgcagcgc
     2221 tggatcgcgc atttcctggc ggtcgacgtc gccatcgcgc cggagcgcga gatcgtcgat
     2281 acgcgctggg tctggcatgt cggactcgac gccgaggcga gcgcgctgct caacgatctg
     2341 tacaacggcg tggacgtcgc cgacgagcgg cggga
//
LOCUS       DATANS010000774         5434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_584877, whole genome shotgun sequence.
ACCESSION   DATANS010000774 DATANS010000000
VERSION     DATANS010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584877"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..599)
                     /locus_tag="VHT22_11520"
     CDS             complement(72..599)
                     /locus_tag="VHT22_11520"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HEX3138234.1"
                     /translation="MQRGLPPVLDAATRVLILGSFPGVASLAARQYYAHPRNHFWPLL
                     GALLDLPLADLTYERRLSALRARRIGLWDSIVRCARAGSADAAIRREELAEIALARRR
                     APQLDLVCFNGQTAARAAPLWREAGYVVCVLPSSSPAYTLPFNEKLAAWRETIVNAAS
                     RRSRRGFKEGTTVRR"
     gene            complement(620..1558)
                     /gene="ubiA"
                     /locus_tag="VHT22_11525"
     CDS             complement(620..1558)
                     /gene="ubiA"
                     /locus_tag="VHT22_11525"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEX3138235.1"
                     /translation="MTVGGPGSGAAAPTRWQAIATRIDAYERLIRLDKPIGTLLLLWP
                     TLSALWLAAFGSPSWIVVGIFALGTLLMRSAGCAINDYADRNFDAHVERTANRPLARG
                     EIAPWEALAVAAAFACCALLLVLPLNRTTILLSVPALLITATYPYCKRFFSLPQAFLG
                     IAFSFGIPMAFASVNNEVTPLGWSLFGINLFWVMAYDTEYAMVDRDDDLKLGLRTSAI
                     TFGRFDVLVVMFCYAVYLTGLTWIGSERQMGPLFYIGVLAASLIALWHYRLIRKGDRA
                     DCFRAFLGNHWLGFAVFAGIVADYGVRLHRMPWWPQ"
     gene            complement(1555..2466)
                     /locus_tag="VHT22_11530"
     CDS             complement(1555..2466)
                     /locus_tag="VHT22_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX3138236.1"
                     /translation="MIRVLLIGCGDIALRTADLLRGKVRLYGLTRRRDEIPKLRGRGI
                     VPIIGDLDRYHSLERLRTAPFAVLHFAPPPAEGRDDPRTASLVAALTKARIIPQRFVY
                     ISTTGVYGDCAGARVSETRPRKPQTPRAKRRVAAEDRLRRWARRYGVSLSVLRAPGIY
                     AETRLPLDRIRQGTPVLRPEDDVFTNHIHADDLARAAVAAAFRGKPNRAYNISDDAEL
                     KLGSWLDAVADAFRLPRPPRVSWDEAEARIAPMLLSFMSESRRLSNTRMKRELRVRLA
                     YATPDALLADIAPRALKKQLALPLGVR"
     gene            2501..3532
                     /locus_tag="VHT22_11535"
     CDS             2501..3532
                     /locus_tag="VHT22_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HEX3138237.1"
                     /translation="MPHQITIKPSEHSFACDDDETVLAAAMRADLMIPYGCRNGACGT
                     CKSHLLEGVVDYGHYQPGTLTDAEKRQGYALLCVARPKTDLVVEVREVRKAGDIRIRK
                     LPCRIEKIDKVAADVAIVSLKLPANDRLQYLAGQYVDFLLKDGRRRSFSIATAPEDDA
                     LLDLHIRHVPGGYFSGQLFNEFKGREILRIEGPLGNFFLREDSDKPIIFVAGGTGFAP
                     IKAVIEHAFHHAIDRQIVLYWGVRSRADLYLPDLPAEWQRRHSNFTFIPVLSDPLPDD
                     RWPGRTGFVHKAVMTDFADLSGYQVYACGGPAMIDVARREFTELRRLPPEAFYADSFT
                     YAAQAEAPA"
     gene            3568..4986
                     /locus_tag="VHT22_11540"
     CDS             3568..4986
                     /locus_tag="VHT22_11540"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEX3138238.1"
                     /translation="MTFDSLTFVVFLAAIFGGYWAVRSWTARKVFLLSASYLFYAAWN
                     PFFVLLIIATTGFDWLASHWMDRVQRPSHRRAILASSIAINLGSLGFFKYAQFFADNA
                     VDLLHVFGINFEPVSLGILLPVGISFYTFESLSYVIDVYRRRIAVSRSLLDYGLYVTF
                     FPHLVAGPILRYGDFSPQCAEPKSWESAEVGKGLALFVYGLALKVCLADLVFAPVVDR
                     VFAPGVHAGIGESWLGATSFSLQAYCDFAGYSLCAIGVALMFGFRFPMNFESPFGSVG
                     MAELWTRWHMSLASWIRDYVFLPLGGYRRGRLRGEANLLFAFLLVGLWHGAAWTFVVW
                     GGLQGAFISAEHLVKRYVWDFGKVDNRFGRAALAVATFGMFSIAAVFFRAHSFGQGAA
                     ILASMFAPGGRSTLVPFSEAIVALVLGACVVGSHILFGHLRRWDWLDRLSVAPRAASI
                     ALALAAVVFSPGFNPAFIYFQF"
     gene            4989..>5434
                     /locus_tag="VHT22_11545"
     CDS             4989..>5434
                     /locus_tag="VHT22_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138239.1"
                     /translation="MNRQWLHAWVAALLVCALLAVGYEWLLRQRGYLVTVQDDADLWS
                     MQLDRIRNSPRAVALLGASRIEFGIDPQLLSHELGGRPVAMLAVNGRYPLAALRELAD
                     DPSFVGLAIVGVDARGMQRRHWEMQQSYFDHYRRRWTLSRWIHRKL"
BASE COUNT          875 a         1807 c         1763 g          989 t
ORIGIN      
        1 cattgcgcgt gcggcggcgt gctgcgcgga cgttgcgcta ggctcgccgg cctggatacc
       61 gccaaggcgc ctcatcggcg cacggtcgtg ccctccttga agcctctccg ggatcgacga
      121 ctcgcggcgt tgactatcgt ctcgcgccag gcggcaagct tctcgttgaa gggcagtgtg
      181 taggccggac tggacgaagg caggacgcag accacgtagc ccgcctcgcg ccatagcggc
      241 gccgcccggg cggccgtctg tccgttgaag cagaccagat cgagctgcgg tgcacggcgc
      301 cgtgccagcg cgatttcggc aagctcctcg cggcggatcg ccgcatcggc actgcccgcg
      361 cgagcacacc gcacgatgct gtcccacagt ccgatccgac gcgcgcgcaa cgcggaaagc
      421 cggcgctcgt acgtcagatc ggccaaaggc aagtcgagca gcgcgccgag caacggccag
      481 aaatggttgc gcggatgcgc gtagtactgg cgcgcggcca gcgaggcgac accgggaaaa
      541 ctgccgagga tcagcacccg cgtcgccgcg tcgaggaccg gcggcaatcc gcgttgcagt
      601 ggcggcgccg gcgcggccct cactgcggcc accatggcat ccggtgcagc cgcacgccgt
      661 aatctgcgac gatgccggcg aacacggcaa agccgagcca gtggttgccg agaaacgcgc
      721 ggaagcaatc cgcgcggtcg cccttgcgta tcagccggta gtgccacaac gcgatcagcg
      781 acgccgccag cacaccgata tagaacagag gtcccatctg ccgctcgctg ccgatccagg
      841 tcaatccggt caggtagacc gcgtagcaga acatcaccac cagcacgtcg aacctgccga
      901 atgtgatcgc cgaggttctc agccccagct tcaggtcgtc gtcgcggtcg accatcgcgt
      961 actcggtatc gtaggccatc acccagaaca ggttgatccc gaacagcgac catccgaggg
     1021 gcgtaacttc attgttcacc gatgcgaacg ccatcggaat gccgaacgag aacgcgatgc
     1081 cgaggaaagc ctgcggcagc gagaaaaagc gtttgcagta cggatatgta gcggtgatca
     1141 gcagggccgg caccgacagc agaatcgtcg tccggttgag cggcagcacc agaagaagcg
     1201 cgcaacaggc gaatgccgcc gcgacggcga gcgcttccca cggcgcaatc tcgccgcgcg
     1261 cgagcgggcg attggcggtg cgctcgacgt gcgcgtcgaa attgcggtcg gcgtagtcgt
     1321 tgatcgcgca gcccgccgag cgcatcaaca gcgtgcccag cgcgaaaatg ccgacgacaa
     1381 tccacgacgg cgacccgaac gccgcgagcc acaacgcgga cagcgtcggc caaagcagca
     1441 gcagcgtgcc gatcggcttg tcgagccgga tcaggcgctc gtaggcgtcg atccgcgttg
     1501 cgatcgcctg ccagcgggtc ggcgcagccg ctccagagcc gggtccgccg accgtcatcg
     1561 cacgcccagc ggcagcgcga gctgcttctt cagcgcgcgc ggtgcaatgt cggccagcag
     1621 cgcgtcgggg gttgcataag cgagtcgcac gcgcaactcg cgtttcatcc gcgtgttgga
     1681 aagccgcctc gattcgctca tgaacgagag cagcatcgga gcgatgcgcg cttcggcctc
     1741 gtcccacgac acgcgcggcg gccgcggcag gcgaaatgcg tcggcgaccg cgtcgagcca
     1801 gctgccaagc ttcagctcgg catcgtcgct gatgttgtac gcgcggttcg gcttgccgcg
     1861 aaacgccgcg gcaacggcgg cgcgcgccag atcgtcggca tggatgtgat tggtgaagac
     1921 gtcgtcttcg ggccgaagca ccggcgtccc ctgccggatg cggtcgagcg gcagcctcgt
     1981 ttcggcatag atgcccggcg cgcgcagcac cgaaagactg acgccgtagc gccgcgccca
     2041 gcggcgcagc ctgtcctcgg ccgcgacgcg cctctttgcg cgcggcgtct gtggcttgcg
     2101 cggacgcgtt tcgctgaccc gtgcgccggc gcaatcgcca tagacgccgg tcgtgctgat
     2161 gtagacgaag cgctgcggta taatccgcgc ttttgtcagc gccgcgacga ggcttgccgt
     2221 tcgcggatcg tcgcggcctt cggccggtgg cggcgcaaaa tgcagcactg caaaaggcgc
     2281 cgtccgcagg cgctcgaggc tgtgatagcg atcgagatct ccgatgatcg gcacgatgcc
     2341 gcggccgcgc agcttcggta tctcgtcgcg ccgacgcgtg agcccgtaca gccgcacctt
     2401 gccgcgcaga agatcggcgg tgcgcagcgc gatgtcgcca cagccaatta gcaagaccct
     2461 gatcaccgcc gtctctacgc tgcgcgctta aagtctaccg atgccgcatc agattaccat
     2521 aaagcccagc gagcattcgt tcgcctgcga cgacgacgag accgtgctcg cggcggcgat
     2581 gcgcgccgac ctgatgattc cctacggctg ccgcaacggc gcctgcggaa cctgcaagag
     2641 ccatctgctc gaaggcgtcg tcgactatgg gcattaccag ccgggcacgc tgaccgatgc
     2701 ggaaaaacga cagggctatg cgctgctttg cgtcgcccgc ccgaagaccg atctcgtcgt
     2761 cgaagtacgc gaagtgcgaa aggccggcga tatccgcatc cgcaagctgc cctgccggat
     2821 cgaaaagatc gacaaggtcg ccgccgacgt cgcgatcgtc tccttgaagc ttccggcgaa
     2881 cgaccgtctg caatacctgg ccggccaata cgtcgatttt ctgctcaagg acggtcgccg
     2941 gcgcagtttc tcgatcgcga ccgcgcccga ggacgacgcg ttgctcgacc tgcatatccg
     3001 gcacgttccc ggcggctatt tcagcggcca gctattcaac gaattcaagg gccgcgagat
     3061 cctgcggatc gaggggccgc tgggcaattt ctttttgcgc gaggactcgg acaagccgat
     3121 catcttcgtc gccggcggca ccggcttcgc accgatcaag gcggtaatcg agcacgcgtt
     3181 tcatcatgcg atcgaccgcc agatcgtgct ctactggggc gtacgctcgc gcgccgatct
     3241 ctacctgccg gatcttccgg ccgaatggca gcgccggcat tcgaacttca ccttcatccc
     3301 ggtgctgtcc gatccgctgc ccgacgaccg ctggccgggc cgcaccggct tcgtacacaa
     3361 agcggtgatg acggacttcg ccgacctgtc cggctatcag gtctacgcct gcggcggtcc
     3421 ggcgatgatc gacgtcgcgc gtcgcgaatt caccgagctg cgacggctgc cgccggaagc
     3481 cttctatgcc gactcgttca cctatgcggc gcaggccgaa gcgccggcgt aggcaaacga
     3541 tagcgagctg acgggccggc ggccgccatg acattcgatt cgctgacctt cgtcgtcttt
     3601 ctcgccgcca ttttcggcgg ctactgggcg gtgcgctcgt ggaccgcgcg caaggtcttt
     3661 ctgctgagcg ccagctatct tttctacgcg gcgtggaacc cgtttttcgt attgttgatc
     3721 atcgcgacga cggggttcga ttggctggcc tcgcattgga tggaccgggt gcagcgcccg
     3781 tcgcaccgcc gcgcgatcct cgcgtcgtcg atcgccatca acctgggctc gctggggttt
     3841 ttcaagtacg cgcagttctt tgccgacaac gcggtagacc tgctgcacgt gttcggaatc
     3901 aatttcgaac cggtgtcgct cggcatcctg ctgccggtcg ggatctcgtt ctacacgttc
     3961 gagagcctgt cgtatgtgat cgacgtctac cgcaggcgca tcgccgtgtc gaggagcctg
     4021 ctcgactacg gcctgtacgt gaccttcttt ccgcacctgg tcgccggacc gatccttcgc
     4081 tacggcgatt tttcgccgca gtgcgctgag cccaaatcct gggaatcggc cgaggtcggc
     4141 aagggcctcg cactgttcgt ctacggcctg gcgctcaagg tctgcctggc cgacctggtg
     4201 ttcgcgccgg tcgtcgaccg cgtgtttgcg cccggcgtcc acgctggaat cggcgaaagc
     4261 tggctgggtg ccacgtcgtt ctccctgcag gcctattgcg acttcgccgg ctattcgctt
     4321 tgcgcgatcg gcgtcgcgct gatgtttggc ttccggtttc cgatgaattt cgaaagcccg
     4381 ttcggttcgg tcggcatggc cgagttgtgg acgcgctggc acatgtcgct ggcgagctgg
     4441 atccgcgact acgtcttcct cccgctcggc ggctatcgcc gcggcaggct gcgcggtgaa
     4501 gccaacctgc tgttcgcgtt cctgctggtt ggcctctggc acggcgcggc atggacgttc
     4561 gtcgtgtggg gcgggctgca gggcgccttt atctctgccg agcacctggt caagcgatac
     4621 gtctgggact tcggcaaggt cgacaaccgc ttcggccgtg cggcactcgc cgtggcaacg
     4681 ttcggaatgt tcagcattgc cgcagtgttc ttccgtgcgc attcgttcgg ccagggcgct
     4741 gcgattctcg cgtcgatgtt tgcccccggt gggcgctcga cgctggttcc gttcagcgag
     4801 gcaatcgtcg cgctggtgct gggtgcctgc gtcgtcggtt cgcatatcct gttcggccac
     4861 ctgcgccgct gggactggct cgatcgcttg tcagtcgcgc cgcgcgcggc ttcgatcgcg
     4921 ctggcgctgg ccgccgtcgt gttctcgccc ggattcaatc cggcgttcat ctatttccag
     4981 ttttgacgat gaaccgacag tggctgcatg cctgggtcgc cgcgctgctg gtgtgcgcgc
     5041 tgctcgccgt cgggtacgag tggctgctcc gccagcgcgg ctatctagtc accgtgcagg
     5101 atgacgccga cctgtggtcg atgcaactcg atcgcatccg gaattcgccc cgcgcggtgg
     5161 cgctgctggg cgcgtcgcgc atcgaattcg gcatcgatcc gcagttgctt tcgcacgagc
     5221 tcggcggccg ccctgtggcg atgctcgccg tcaatggcag atacccgctg gcggcgctgc
     5281 gcgagctggc cgacgaccct tcgttcgtcg ggctcgcgat cgtcggcgtc gatgcacgcg
     5341 gcatgcaacg ccggcattgg gaaatgcaac agagctattt cgaccactac cgccgtcgct
     5401 ggacgctttc gcgctggatc catcgcaaat tgtt
//
LOCUS       DATANS010000775         7982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_38670, whole genome shotgun sequence.
ACCESSION   DATANS010000775 DATANS010000000
VERSION     DATANS010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 7982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7982
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38670"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VHT22_11550"
     CDS             complement(<1..506)
                     /locus_tag="VHT22_11550"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEX3138240.1"
                     /translation="MATQAAVRNISGLARAMVQQGLLSEFDADALQTQAKAANLGFVE
                     QVLVSKRMTAPQIAVFASRAFHAPLFDIAGFDIDQVNKELIDPKMIVTRRILPLQKRG
                     NRLFVAVSDPANLQALDEIRFKTNLVIDAVVVEDDKLAQTITKVVEAMGQNLKDLVSA
                     EDLEIDLA"
     gene            complement(568..1884)
                     /locus_tag="VHT22_11555"
     CDS             complement(568..1884)
                     /locus_tag="VHT22_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEX3138241.1"
                     /translation="MDDIPLSTLVISLVALLAVSGFFSIAETAMMAVNRHRLRLKAKH
                     GERGAKVALGLLARTDKLLGVILLGNTLVNAIATTLTAVITTRLFGQSEASLMVGGVA
                     IALAILIFSEITPKVVGAAYADRIAPPVGFLLAPMLRLSYPIVWFVNLFVQGLLILLR
                     LKPHEEIEARLSQEEVRSLVLEGHFFRGKHRAMLANLFDLASVSVDDVMTPRNQIEAI
                     DLNEKPALLAQQLATSYHTMLAVYENSLDNIVGLLHLKQVLRLRQAEEIDAEQLREIL
                     RAPYFIPAGTPLLTQLHQFQVDRQRLGLVVDEYGELKGLVSIEDILEEIVGEFTTQGP
                     SGSDVFRREPDGSVIVDGMAPLRMLNRKLGTEFPLEGPKTLNGLIVEYLGEIPDSGIS
                     FRLAGQGVEVLQVQDRAVKVVKLLPPVGRAPAPPLQTTGEQASSIS"
     gene            complement(1957..2766)
                     /gene="ccsA"
                     /locus_tag="VHT22_11560"
     CDS             complement(1957..2766)
                     /gene="ccsA"
                     /locus_tag="VHT22_11560"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX3138242.1"
                     /translation="MILLYLAVAAAYLAATWLEWRHLAQAPEPASSATAIARWLPALA
                     LAGHAVLVSSVVFAGGSVDLSLANALSAVAGLIALFAWLGSRAGTLPGVAVIALPVAA
                     VAALLPLLFANPHRMSLSDEPWAALHIAVALTAYAMLIVAGLQALLLMGFQRRLHRGV
                     AVRGGAEMPPLLTLERFLFRLVSLGYALLTLTLLSGIVFSEELFGKPLTLTHKVVFSV
                     LGWLVFGALLVGRYRYGWRGKVALRWILVGSVLLLLAYIGSKFVLEAILHR"
     gene            2841..4190
                     /gene="ffh"
                     /locus_tag="VHT22_11565"
     CDS             2841..4190
                     /gene="ffh"
                     /locus_tag="VHT22_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX3138243.1"
                     /translation="MLDNLTQRLGRVAKTLRGEARLTDANIQEALREVRMALLEADVA
                     LPVVRDFIAIVREKAIGSDVAGSLTPGQAFVGIVQRELTRLIGGPTAPLDLAVVPPAV
                     ILLAGLQGSGKTTSAGKLARLLRQDMRKKVLLVSCDVYRPAAIEQLKTLAGQVGVDFF
                     PSSTDQAPVDIAVASVDWARRHYHEIVIVDTAGRLAIDDAMMREIAALHAAVKPVETL
                     FVVDAMQGQDAVNTAKAFGAVLPLTGVILTKLDGDARGGAALSVRQVTGAPIKFAGVS
                     EKLDGLEVFHPERMAMRILGMGDVLGLIEGAQRQVDVADAQKLAKKVKSGKGFDLEDF
                     KAQIGQMRKMGGMQELIDKLPGELARAAQGTQVDDGAIRRLEGIINSMTPAERAKPEL
                     LKASRKRRIAAGAGASVQEVNRLLAQFEQTQKMMKMVSKGGIQKMMRGLKGAFPGLR"
     gene            4285..4542
                     /gene="rpsP"
                     /locus_tag="VHT22_11570"
                     /pseudo
     CDS             4285..4542
                     /gene="rpsP"
                     /locus_tag="VHT22_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003268924.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
     gene            4661..5149
                     /gene="rimM"
                     /locus_tag="VHT22_11575"
     CDS             4661..5149
                     /gene="rimM"
                     /locus_tag="VHT22_11575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX3138244.1"
                     /translation="MGRLLAPFGIKGWVRLKPFTAAPETLLAYKTWWLAPDESKEWTR
                     YRVAEAQIHGGSIIAAIEGIASREAAQGLRGFVAGVPRKSLPKTAPDEHYWSDLVGLN
                     VVNRAGRTLGRVAGLLDTGAHPVLRVESDGAERLIPMVPAYVDTIEPAAGRIVVDWQE
                     DY"
     gene            5194..5964
                     /gene="trmD"
                     /locus_tag="VHT22_11580"
     CDS             5194..5964
                     /gene="trmD"
                     /locus_tag="VHT22_11580"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX3138245.1"
                     /translation="MVRIEVVTLFPEMVQGAAAYGVMGRALERGIWTLGCRNPRDFAT
                     DSYRTVDDRPFGGGPGMVMRAEPLTKALDAARRDMQAAGVERSPVVYLSPQGEPLRHA
                     RVAKLAAEPGLILLAGRYEGVDERLLEKEIDLEVSIGDFVVSGGELPALLLIDAIVRQ
                     LPGVLNDAQSNVEESFVDGLLDCPHYTRPEQLEGGVVPAVLLSGNHAEIARWRRKQSL
                     GRTWLRRPDLLAEKSLSAEDTKLLEEFKREGGGRAADK"
     gene            6085..6462
                     /gene="rplS"
                     /locus_tag="VHT22_11585"
     CDS             6085..6462
                     /gene="rplS"
                     /locus_tag="VHT22_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX3138246.1"
                     /translation="MNIIDTLDKEEMARLGKTVPDFAPGDTVIVNVNVVEGERKRVQA
                     YEGVVIAKRNRGLNSSFVVRKISSGEGVERSFQTYSPLIASIEVKRKGDVRRAKLYYL
                     RDRSGKSARIREKLAHREDKGSE"
     gene            6799..>7982
                     /locus_tag="VHT22_11590"
     CDS             6799..>7982
                     /locus_tag="VHT22_11590"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX3138247.1"
                     /translation="MKATLYEALGVPQLAPDEEVRAALRRLIRKYYAKTRDGQGNVEE
                     ALRFINHASRILSDPEHRQHYDQELAVAAEGSTEQRISRFVSNVAATAEAEGTAAHDE
                     DEVIEAADEKLLTTAIGAPDKSLHHPGLTERVASFGRSAMGQLALCLLFGAFITGAIV
                     FVTPADSVLVAKQVLVWLTLLLLVLSLIYGVVHGLSWVRRGHVPRIPALIPQTDLAIL
                     NWRREKSVFLGTDAPQEDASWIFQLRMAELERAKSGRTSEPRPWNRLAARLFDYAIWG
                     LVLALLLSELHNAGVVSDDVAFWLGHPLLAPMLITASWIPIESLLIASLQQTPGKWLF
                     GVYLQFSISNAYARRDVRAQLQRALRRSFHVWWAGLACGFPLFAPVLIALAYEKVAQQ
                     GET"
BASE COUNT         1466 a         2500 c         2699 g         1317 t
ORIGIN      
        1 tcggccaggt cgatttcgag gtcttcggcg ctgaccaggt ccttcaggtt ctggcccatt
       61 gcctcgacga ccttggtgat cgtctgggcg agcttgtcgt cctcgacgac gacggcgtcg
      121 atgaccaggt tggtcttgaa gcggatctcg tcgagcgcct gcaggttggc cgggtccgag
      181 acggcgacga acaggcggtt gccgcgtttt tgcagcggca gaatacggcg cgtcacgatc
      241 atcttcggat cgatcagctc cttgttgacc tggtcgatgt cgaagccggc gatatcgaac
      301 agcggggcgt ggaacgcgcg cgacgcgaaa acggcgatct gcggagcggt catccgcttg
      361 ctgacgagga cctgctcgac aaagccgaga ttggcggcct tcgcctgggt ctgcagcgcg
      421 tcggcgtcga attcggacaa caagccctgc tggaccatcg ctcgggccag gccgctgatg
      481 ttacggactg ctgcttgcgt tgccatagtc ggtcggcgtt tgcaggggta aatccccccg
      541 aaaaccttta ttccagaggc atataagtta acttatcgat gatgcctgct ccccggtggt
      601 ttgtaaaggc ggcgccgggg cacgcccgac tggaggcaac aacttgacca ccttgaccgc
      661 ccgatcctgg acctgcagca cttcgacccc ctggccggcc agccggaaac tgatgcccga
      721 gtcggggatt tcgcccagat attcgacgat caggccgttg agcgttttcg ggccctcgag
      781 cggaaattcg gtgccgagct tgcggttgag catccgcagc ggcgccatgc cgtcgacgat
      841 gacgctgccg tcgggctctc ggcgaaacac gtccgacccg ctcgggcctt gcgtggtgaa
      901 ttcgccgacg atctcctcca ggatgtcctc gatcgacacc aggcccttga gctcgccgta
      961 ttcgtctact acaagtccga ggcgctgccg gtcgacctgg aactggtgga gctgggtcag
     1021 caacggcgtt ccggccggga tgaagtaggg cgcgcgcagg atctcgcgca gctgctcggc
     1081 gtcgatctcc tcggcctggc gcaggcgcag cacctgcttc agatgcaaca ggccgacgat
     1141 attgtccagt gagttctcgt acaccgccag catggtgtga tagctggtcg cgagctgctg
     1201 ggcgagcagg gccggcttct cgttcagatc gatcgcctcg atctggttgc gcggcgtcat
     1261 cacgtcgtcg acggaaaccg acgccagatc gaacaggttg gcgagcatcg cgcgatgctt
     1321 gccccggaag aagtggccct cgagcacgag gctgcgcact tcctcctgcg acagccgcgc
     1381 ctcgatctct tcgtggggct tgagcctcag cagtatcaac aggccctgca cgaacaggtt
     1441 gacgaaccac acgatcgggt acgacagacg cagcatcggc gcgagcagaa aacccaccgg
     1501 cggggcgatg cgatcggcat acgcggcgcc aacgaccttc ggcgtgattt ccgaaaagat
     1561 caggatcgcc agcgcaatcg ccacgccgcc gaccatcagc gacgcctcgc tctgtccgaa
     1621 caggcgcgtc gtaatcaccg ccgtcagcgt ggtcgcgatc gcgttgacca gcgtattgcc
     1681 cagaagaatg acgccgagca gcttgtcggt gcgggcgagg agtcccagcg cgactttggc
     1741 gccgcgctcg ccgtgcttgg ctttcaaccg cagtcgatgg cggttgacgg ccatcattgc
     1801 cgtctcggcg atcgagaaaa agcccgaaac cgcaagcagc gcgaccagcg agatgaccag
     1861 cgtcgacagc ggaatgtcgt ccaagcagcg cgcgccgtga aaggatggag atcagtattt
     1921 ggcgcgcggg gagcgctgtc aaggcgacgg cgcttatcag cggtgcagta tcgcctcgag
     1981 cacgaacttg ctgccgatgt aggcgagcag caacagcacg ctgccgacga ggatccagcg
     2041 cagcgcgact tttccgcgcc agccgtagcg gtagcggccg accagcagcg cgccgaacac
     2101 cagccagccg agcaccgaga acacgacctt gtgcgtcaag gtcagcggct tgccgaacaa
     2161 ttcctcggaa aaaacgatgc cgctcagcag ggtcagcgtc agcagcgcat aaccgaggct
     2221 gaccagccgg aacaggaagc gctcgagcgt cagcagcggc ggcatttccg ccccgccgcg
     2281 cacggccacg ccgcgatgca aacgccgctg gaagcccatc agcaacaacg cctgcagccc
     2341 cgcgacgatc agcatcgcgt acgcggtaag cgccacggcg atgtgcaagg cggcccacgg
     2401 ttcgtcggag agcgacatcc ggtgcggatt ggcgaacagc agcggcaaca acgccgcgac
     2461 cgccgcgacc ggcagcgcga tcacggcgac gccgggcagc gtacccgcgc gcgagccgag
     2521 ccatgcgaac aatgcgatca ggccggcgac tgcggacagc gcattggcga ggctcaagtc
     2581 gacgctgccg ccggcgaaca cgacgctcga tacgagcacc gcgtgtccgg cgagcgcgag
     2641 cgcgggcagc catcgggcga tcgcggtagc ggacgatgca ggctccggcg cttgcgcgag
     2701 atgccgccat tcgagccagg tggcggcgag gtaggcggcg gcgaccgcca ggtagagtaa
     2761 aatcatgttt gtttttggca gtctacacca agggcgcgcc ggttcccggc agcgcttttc
     2821 gcaacgcgaa tccatcgacg atgctcgaca atctgaccca acggctaggc cgcgtcgcca
     2881 agacgctgcg cggtgaagcg cggctcaccg acgccaacat ccaggaagcg ctgcgcgaag
     2941 tcaggatggc gctgctcgag gccgacgtcg cgcttccggt cgtccgcgac ttcatcgcga
     3001 tcgtgcgcga aaaagcgatc ggcagcgacg tggccggatc gctgaccccg ggacaggcgt
     3061 tcgtcggcat cgtccagcgc gaattgacgc ggctgatcgg cggcccgacg gcgccgctcg
     3121 atctcgccgt cgttccgccg gcggtgatcc tgctcgccgg actgcaaggc tccggcaaga
     3181 ccaccagcgc ggggaagctc gcgcggctgt tgcgccagga catgcgcaag aaagtgctgc
     3241 tggtgagctg cgacgtctac cgtcctgcag cgatcgagca gctcaagacg ctggccggcc
     3301 aggtcggcgt cgatttcttc ccgtcgtcga ccgaccaagc tccggtggac atcgcggttg
     3361 cgagcgtcga ctgggcgcga cgtcactatc acgagatcgt catcgtcgac accgccggac
     3421 ggctggccat cgacgacgcg atgatgcgcg agatcgccgc cttgcacgcc gcggtcaagc
     3481 cggtcgagac gctgttcgtc gtcgacgcga tgcagggcca ggatgcggtc aatacggcga
     3541 aggcattcgg cgctgtgctg ccgctgaccg gcgtcatcct gaccaagctc gacggcgatg
     3601 cgcgcggcgg cgcggcgctt tcggtgcgtc aggtgaccgg ggcgccgatc aagttcgccg
     3661 gcgtgtccga aaagctcgat gggctcgaag tgtttcatcc cgagcgcatg gcgatgcgca
     3721 ttctcggcat gggcgatgtg ctgggcctga tcgaaggggc gcagcggcag gtcgatgtcg
     3781 ccgacgcgca aaagctcgcc aagaaggtca agtccggcaa aggcttcgac ctcgaggact
     3841 tcaaggcgca gatcgggcag atgcgcaaga tgggcggcat gcaggagctg atcgacaagc
     3901 tgcccggcga gctcgcgcgc gccgcacaag gcacacaggt cgacgatggc gccattcgcc
     3961 gcctcgaggg catcatcaac tcgatgaccc cggccgagcg cgccaagcca gagctcctga
     4021 aagcatcgcg caagcgccgg atcgcggccg gcgccggcgc gtcggtgcag gaagtcaatc
     4081 gcctgctcgc ccagttcgag cagacgcaga aaatgatgaa aatggtctcc aagggcggca
     4141 tccagaagat gatgcggggc ctcaaaggcg cctttccggg cctgcgctag gacagtccgg
     4201 gcgcgtcgcg ccgccgattg cgggcagaac ggatttcgtc gtataatcaa cgtctttttt
     4261 tgcggctcaa cccgggactt tgccatggtc gtaatcagac tcgctcgagg cggcgccaag
     4321 aagcgcccgt ttttcaacgt cgtcgtcgcc gattcgcgca accgccgcga cggacggttt
     4381 atcgagcgcg tcggtttcta caatccgatc gctcgcgaag gccaggagag cctgcgcctg
     4441 tcgctcgatc gtatcgacca ctggacgcat cagggcgcca agctgtccga taccgtcgcc
     4501 ggcctggtca agcaggcgtc ggcggcgaag cctgccgcag cggctacgcc aactgccgcc
     4561 gccgccgccg cgccgactgc cgaagccgcc gcgccgaccg ctggctagca agcgcggcgc
     4621 cggccgcgac gacgcggtcg cgcccaccga catcgtggtg atggggcgct tgctcgcccc
     4681 gttcggcatc aagggatggg tgaggctcaa gccgttcacc gcggcgccgg aaacgctgct
     4741 cgcgtacaag acgtggtggc tcgcaccgga cgagagcaag gaatggacgc gttatcgcgt
     4801 agccgaagcg caaattcacg gcggctcgat catcgccgcg atcgaaggca ttgcgagtcg
     4861 ggaagcggcg caggggctgc gcggatttgt cgccggcgtg ccgcgcaagt cattgcccaa
     4921 gaccgcgccg gacgagcatt actggtcgga cctggtcggt ttgaatgtcg tcaaccgcgc
     4981 cggtcggacg ctgggccgcg tggccggatt gctggatacc ggagcgcatc cggtgctgcg
     5041 cgtcgaaagc gacggggccg aacgcctgat tccgatggtg ccggcgtatg tcgatacgat
     5101 cgagccggcg gctggccgga ttgtcgtcga ctggcaggaa gactattgaa gcaaggacgc
     5161 ggcgaatcga gagaagagcg cgccgcggcg agcatggtca ggatcgaagt cgtcacgctg
     5221 tttccggaga tggtgcaggg cgcggcggcg tacggcgtca tgggacgtgc gctcgagcgc
     5281 ggcatctgga cgctgggctg ccgcaatccg cgcgactttg ctaccgacag ctatcggacg
     5341 gtcgacgacc ggccgttcgg cggcgggccg gggatggtga tgcgggcaga gccgctgacc
     5401 aaggcgctgg acgcggcgcg gcgagacatg caggccgcgg gtgtcgagcg ctcgcccgtc
     5461 gtctacctgt cgccgcaagg cgagccgttg cgtcatgcgc gcgtggcaaa gctcgcggcg
     5521 gagccggggt tgatactgct cgcgggacgc tacgagggcg tcgacgagcg cttgctggaa
     5581 aaggagatcg acctcgaagt ctcgatcgga gattttgtcg tgtcgggcgg cgaattgccg
     5641 gcgctgctgc tgatcgatgc gatcgtgcgg cagcttccgg gcgtgctgaa cgacgcgcaa
     5701 tcgaacgtcg aggaatcgtt tgtcgacggg ctgctcgatt gtccgcatta cacgcggccc
     5761 gagcagctgg aaggcggcgt ggtgccggcg gtgctgttgt cgggaaacca cgccgagatc
     5821 gcgcgctggc ggcgcaagca gtcgctgggg cgcacctggc tgcggcgacc ggatttgctg
     5881 gcggagaaaa gcctgtcggc ggaagatacg aagttgctcg aggaattcaa gcgcgagggc
     5941 ggcggaaggg cggccgacaa gtagcgctag caacaagcag cagttttacg agtacgggat
     6001 ttccaaaaca aacagcgcgc atcaggtggc gacctgcgct gcgcgccagg ccaacggccg
     6061 ggaccattga tcaaggagca acgcatgaac atcatcgata cgctggacaa ggaagaaatg
     6121 gcccgtctgg gcaagacggt gccggatttc gcgccgggcg acaccgtgat cgtcaacgtc
     6181 aacgtcgttg aaggtgagcg caagcgcgtg caggcctacg aaggcgtggt catcgccaag
     6241 cgcaaccgcg gcctcaattc gtcgttcgtc gtgcgcaaga tctcgagtgg cgaaggcgtg
     6301 gagcggtcgt tccagaccta ctcgccgctg atcgcgagca tcgaagtcaa gcgcaagggc
     6361 gacgtgcgtc gcgccaagct gtactacctg cgtgatcgct cgggcaaatc ggcgcgcatt
     6421 cgcgagaagc tcgcccaccg tgaggacaaa gggagcgaat aacttcttgg cgagcgaaaa
     6481 aaaagcggcc cctgcggccg ctttttttca caagcgacgc ccaccgtgag gacaagtcga
     6541 gcgaataggc tctcttgcct ggcaaaaaaa gcggcccgtg cggccgcttt ttcattggta
     6601 gccgacatat tgagcgccgg gcggcccagg tcctcgccgg cgatcctggc gccgatgctg
     6661 ccgcacagcg gcatcgtctg ccgccccgcg tgtgccggaa cttggcgttt tcgggggtaa
     6721 ttatcgataa cttgcattgt ccaccggttt cccggaacat tccaacgtca gattgcgtgc
     6781 attttcctgc gattggcgat gaaggcaacg ctctacgagg cattgggagt accgcagctc
     6841 gcaccggacg aagaggtgcg ggccgcgctg cgtcgactga tccgaaaata ttatgcgaaa
     6901 acacgcgacg gccaaggcaa cgtagaagag gccttgcgtt tcatcaatca tgccagccgc
     6961 atactaagcg atcccgagca caggcagcat tacgatcaag agctcgccgt tgccgccgag
     7021 ggcagcaccg agcagcgcat ttcgcgcttc gtcagcaacg tcgccgccac tgccgaggct
     7081 gagggtacgg cggcgcacga tgaagacgaa gtcatcgagg ccgccgacga gaagctgctg
     7141 accacggcga tcggggcgcc ggacaagagc ctgcatcacc ccggactgac cgagcgtgtc
     7201 gcgtcgttcg ggcgctcggc gatgggccag ctcgcgctgt gcctgctgtt cggcgcgttc
     7261 atcaccggtg cgatcgtgtt cgtgacgccg gccgattcgg tgctggtcgc caagcaggtg
     7321 ctggtctggc tgacgctgct gctgctcgtg ctctcgctga tctacggcgt cgtgcacggc
     7381 ctctcgtggg tgcgccgcgg tcacgtgccg cgcattccgg cgctgattcc gcagaccgat
     7441 ctcgcgattc tcaactggcg ccgcgagaag agcgtgtttc tcggcaccga cgcgccgcag
     7501 gaagacgcga gctggatatt ccagctgcgc atggccgagc tcgaacgggc gaagtcggga
     7561 cgcaccagcg agccgcgccc gtggaatcgc ctggcggcac gcctgttcga ctacgcgatc
     7621 tggggactgg tgctggcgct gctgttatcg gagctgcaca acgccggcgt cgtcagcgac
     7681 gacgtcgcct tctggctcgg ccatccgctg ctggcgccga tgttgatcac ggcctcatgg
     7741 attccgatcg aatcgctgct gattgcatcg ctgcagcaga cgccgggcaa atggctgttc
     7801 ggcgtctacc tgcaattctc gatcagcaac gcctacgcgc gacgcgacgt gcgtgcgcag
     7861 ctgcagcgcg cgttgcgccg ctcgttccac gtctggtggg caggtctcgc ctgcggattc
     7921 ccgctgttcg cgccggtgct gatcgcgctt gcctacgaaa aagtggcgca gcagggcgag
     7981 ac
//
LOCUS       DATANS010000776         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_80028, whole genome shotgun sequence.
ACCESSION   DATANS010000776 DATANS010000000
VERSION     DATANS010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80028"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="VHT22_11595"
     CDS             <1..1179
                     /locus_tag="VHT22_11595"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3138248.1"
                     /translation="FGGQVTFLALPLTAALLLQATPLQMGILVALETLPFALFSLHAG
                     VLLDRVRKLPVIIAADVGRGIALLAIPVAAWFGLLSMEILFAVGFLCGMQNVVGGAAY
                     QVLLAQMAGRKRLVEANAKVTLGETSAALIGPGIAGGLIHVLTAPFAIALDAMTFFAS
                     ALMLRRIKAPNDVARDGANGGVWREIGEGLKLVWGNRTLWGLAWLAGIWQFLHHMQVA
                     VLILFATRELGLSAGAIGLAFVFGGAGCVLASASAERLSARFGIGPVIVHGLILSAFG
                     WQAYGLIGGPPWLAGLLLGMAMLVFDFGAILYAINYLSLRQAITPDRLLGRMTATMRF
                     VTVASAPIGSLVGGALASEIGLRSTLLVVGVLGLLLSAAAVLWSPVRRHRALPDPLID
                     "
     gene            1291..>2008
                     /locus_tag="VHT22_11600"
     CDS             1291..>2008
                     /locus_tag="VHT22_11600"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX3138249.1"
                     /translation="MPPSILFVTLDSCRYDAFVEAKTPNLSAIGPLVRAQAPSHFTYA
                     SHAAYFMGFTPGDPLRREPYANPKYGKIFRMGGGGDPGVVAPYMTLKGRNIVDGLKNK
                     GYRAIGSGAVNWFNPEQPTGRVLTDDFDVFGYAPGANVKKQIKWIDDALAQVKPDAPP
                     FVFLNLAETHVPYWHEGAPWNRGYNPSVPFGNNNDAAEARRRQIACIEYCDAALVPLL
                     ERFADGTVIVCADHGDCWGED"
BASE COUNT          283 a          658 c          689 g          378 t
ORIGIN      
        1 ttcggcggcc aggtcacgtt cctcgcgctg ccgctgacgg ccgcgctgct cctgcaggcg
       61 acgccgcttc agatgggcat cctggtggcg ctcgagacgc tgccgttcgc gctgttcagc
      121 ctgcacgcgg gcgtgctgct cgaccgggtg cgcaagctgc cggtgatcat cgccgcggac
      181 gttgggcggg gtatcgcgct gctcgcgatt ccggtcgccg catggttcgg cttgctctcg
      241 atggaaatcc tgtttgccgt cgggttcctg tgcggcatgc agaacgtcgt cggcggcgcg
      301 gcctaccagg tgctgctggc gcaaatggcg ggccgcaagc gcctagtcga ggccaatgcc
      361 aaggtgactt tgggcgaaac ctcggcggcg ctgatcggtc ccggtatcgc tggcgggctg
      421 atccatgtgc tgaccgcgcc gttcgcgatc gcgctcgatg cgatgacgtt cttcgcctct
      481 gccctgatgc tgcgccggat caaggcgccc aacgatgttg cgcgcgatgg cgctaacggc
      541 ggcgtctggc gcgagatcgg cgagggattg aagctcgtct ggggcaaccg gacgctctgg
      601 ggcctcgcct ggctggcggg aatatggcag ttcctgcatc acatgcaggt cgccgtgctg
      661 atcctgttcg cgacgcgcga actcgggctg tcggccggag cgatcggcct ggccttcgtc
      721 ttcggcggcg ccgggtgtgt gctggcgtcg gcttcggccg agcgcctgtc ggcgcgcttc
      781 ggtatcgggc cggtgatcgt ccacgggttg atcctgtccg ctttcggctg gcaggcgtac
      841 ggcctgatcg gcggaccgcc gtggctcgcc ggcttgctgc tcggcatggc gatgctggtg
      901 ttcgacttcg gcgcgatcct gtacgcgatc aactatctgt cgctgcggca ggcgatcacg
      961 cccgaccgcc tgctcggacg catgaccgcg acgatgcgct ttgtcaccgt cgcttccgcg
     1021 ccgatcggct cgctggtcgg gggcgcgctg gcaagcgaga tcggcctgcg gtcgacgttg
     1081 ctcgtcgtcg gcgtgctcgg actgctgctg tcggccgccg cggtgctctg gtcgccggtg
     1141 cgtcggcacc gcgcgctgcc cgacccgctg atcgactagc agccgaggcg gcgcgcagtg
     1201 gcgtgaggtg ccgcctcgtg ctcacgccct gcggtaaaat gcgaggatga gccgcatccg
     1261 aatacccgtg cggcggaccc tcaaaaatcc atgccgccct ctatcctgtt cgtcacgctc
     1321 gactcctgcc gctacgatgc cttcgtcgaa gcgaagacgc cgaacctgag cgcgatcggc
     1381 ccgctggtgc gtgcgcaggc gccttcgcat ttcacctacg cctcgcacgc cgcgtatttc
     1441 atgggattca ctcccggcga tccattgcgc cgcgagccgt acgccaatcc caagtacggg
     1501 aagatctttc gcatgggggg cggcggcgat ccgggcgtcg tcgcgcccta catgacgctg
     1561 aaggggcgca acatcgtcga cggccttaag aacaaaggct accgcgccat cggcagcggt
     1621 gcggtgaact ggttcaatcc cgagcaaccg accggacgtg tgctgaccga cgacttcgac
     1681 gtttttggct acgcgcccgg tgccaacgtg aaaaagcaga tcaagtggat cgacgacgcg
     1741 ctcgcgcagg tcaagccgga cgcgccgccg ttcgtctttc tcaacctggc cgagacgcac
     1801 gtgccctact ggcatgaagg cgcaccgtgg aatcgcggct acaacccgag cgtgccattc
     1861 ggcaacaaca atgatgccgc cgaggcgagg cggcgccaaa tcgcatgcat cgaatactgc
     1921 gacgcggcgc tggtgccgct gctcgagcgt tttgccgacg gcacggtgat cgtctgcgcc
     1981 gatcatggtg actgctgggg tgaggacg
//
LOCUS       DATANS010000777         3748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_834366, whole genome shotgun sequence.
ACCESSION   DATANS010000777 DATANS010000000
VERSION     DATANS010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834366"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..856)
                     /locus_tag="VHT22_11605"
     CDS             complement(11..856)
                     /locus_tag="VHT22_11605"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX3138250.1"
                     /translation="MLKTRIITALVLIPLTLAALFGLPPRAWGAVTLAVVVVAAAEWA
                     ELAALERRGWLFFVGTTLFAGCVLLLDPAAGFTSERGWPAPMVSWICGAATVFWLLVA
                     PAWLVSGRRMTSRPVLAIVGWLVLIATWVAVVALQTRSPGVLLAMMAIVWIADTAAYF
                     TGRRFGRRKLAPSISPGKTWEGVYGALAAVAIYALLLLPLAAPAGSTRPLDAIAAAIW
                     VALALLLTGLSIIGDLFESQLKRQRGVKDSGAILPGHGGVLDRIDALTAAMPPAALIA
                     HHLLR"
     gene            complement(862..1626)
                     /gene="uppS"
                     /locus_tag="VHT22_11610"
     CDS             complement(862..1626)
                     /gene="uppS"
                     /locus_tag="VHT22_11610"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX3138251.1"
                     /translation="MIATLFTSSTREVPEVRAVPRHIAIIMDGNGRWAKQRMLPRVAG
                     HRKGVEAVRATVRGCIEQGVEYLTLFAFSSENWRRPADEVSILMQLFLRALEQEVAKL
                     DDNDIRFKVIGDISRFEPKIRELIAAGEAVTEGNSRLTLTVAANYGGRWDVAQAVQKL
                     LATEPGALRGFAPEALEPYLSMAYAPEPDLFIRTGGEQRISNFLLWQLAYTEFFFTDM
                     LWPDFDAEALCAAIDSYRQRERRFGRTSEQLQAVRD"
     gene            complement(1623..2180)
                     /gene="frr"
                     /locus_tag="VHT22_11615"
     CDS             complement(1623..2180)
                     /gene="frr"
                     /locus_tag="VHT22_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX3138252.1"
                     /translation="MIADVKKAAEQKMQKSVEALKTDLGKVRTGRAHTGLLDHIKVDY
                     YGTMMPINQVANVTLADAHTITVQPWEKKMVHPVEKAIRDSDLGLNPATSGDLIRVPM
                     PALTEERRKELIKVVHREAENARVAVRNIRRDAISHLKDLLKAKSVAEDDERRAQDDV
                     QKLTDRYIADVDKLLHAKEADLMAV"
     gene            complement(2287..3051)
                     /gene="pyrH"
                     /locus_tag="VHT22_11620"
     CDS             complement(2287..3051)
                     /gene="pyrH"
                     /locus_tag="VHT22_11620"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX3138253.1"
                     /translation="MTPQNIAPVSNVAPLPRTEPAYKRILLKLSGEALMGNDPYGINR
                     EVIDRIVAEIGEVVRLGVQVAVVIGGGNIFRGVAPGAAGMDRATADYMGMLATLMNAL
                     ALQDAMRRAGLVSRVQSALSIEQVAEPYIRGKALRYLEENKIVIFAAGTGNPFFTTDT
                     AAALRGREMGVDIVLKATKVDGVYTADPNKDQSARRYPTLTFDEAIVKNLKVMDATAL
                     ALCRDQNMLLKVFSIFAPGALKRVVMGEDEGTLVHC"
     gene            complement(3079..>3748)
                     /gene="tsf"
                     /locus_tag="VHT22_11625"
     CDS             complement(3079..>3748)
                     /gene="tsf"
                     /locus_tag="VHT22_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX3138254.1"
                     /translation="ALLELNCETDFVARNADFIAFAGKLAALVVEKNPAGVAALLALP
                     LGDGTVESARQALVQKLGENISVRRFSRVATPARLAQYLHGGRIGVMVEIEGGDEKTG
                     KDVAMHIAASVANVRPVCVSRDQVPAELIDKERAIYSAQAADSGKPAEIVAKMVEGRI
                     NKYLAEVTLLGQPFAIDTEQTVEKYLKSKGATVRGFTLYVVGEGIERKKDDFAAEVAA
                     MAKA"
BASE COUNT          666 a         1263 c         1148 g          671 t
ORIGIN      
        1 atgggagcga tcatctgagc aggtggtgcg caatcaacgc agcaggcggc atggcggcgg
       61 tcagcgcatc gattcgatcg agcacgccac cgtggccagg cagaatggcc ccactatcct
      121 tgacaccgcg ctgacgtttc aactgcgatt cgaacaggtc gccgatgatc gacagtcccg
      181 tcaacagcag cgccagagcg acccagatcg ccgcagcaat cgcgtcgagc ggccgcgtgg
      241 agccggctgg cgccgcgagc gggagcagca gcaacgcgta gatcgccacc gcagcgagcg
      301 caccatagac gccctcccag gtcttgccgg gactgatcga cggtgcgagc ttcctgcggc
      361 cgaaacgccg gccggtaaag taggcagcgg tatcggcaat ccacacgatc gccatcatcg
      421 ccagcagcac gcccggcgag cgcgtctgca gagcgaccac cgccacccag gtcgcgatca
      481 gcaccagcca gccgacgatc gcgagcacag gccgcgatgt catgcgccgt cccgagacca
      541 accacgccgg cgcgaccagc aaccagaaca cggtagcagc gccgcaaatc cacgacacca
      601 tcggcgccgg ccagccgcgc tccgacgtga agcccgcggc gggatcgagc agcagcacgc
      661 acccggcgaa aagcgtcgtg ccgacgaaaa acagccagcc gcgcctctcg agcgcggcga
      721 gttccgccca ttcggctgcc gcgacgacga cgacggcgag cgtcactgca ccccaggcgc
      781 gcggcgggag cccgaacagc gcggcgagcg tcagcggaat caggaccagc gcggtgatga
      841 tgcgggtctt cagcacccgg ctcaatcgcg cacagcttgc agttgctcgc tggtccgtcc
      901 aaaccgacgc tcgcgctgcc gataggaatc gatcgccgcg cacagcgctt cggcatcgaa
      961 atcgggccac agcatgtcgg taaagaagaa ttcggtgtag gcgagctgcc acaacaagaa
     1021 attcgatatg cgttgctcgc cgccggtgcg tatgaacagg tccggctccg gcgcgtaggc
     1081 catcgataaa tacggttcca gcgcctcggg cgcgaagccg cgcagcgcgc ccggctcggt
     1141 agccagcagc ttctgcaccg cctgcgccac gtcccagcgc ccgccgtagt tggcggcaac
     1201 cgtcagcgtc aaacgcgagt tgccttcggt cacggcctct ccggcggcga tcagctcgcg
     1261 tatctttggc tcgaatcgcg aaatgtcgcc gatcaccttg aaccgtatgt cgttgtcgtc
     1321 gagcttggcg acttcctgtt ccagcgcacg caggaacagc tgcatcagga tcgacacttc
     1381 gtcggcggga cggcgccagt tctcgctgga gaacgcgaaa agcgtcaggt actcgacccc
     1441 ctgctcgatg cagccgcgga ccgtcgcccg cacggcctcg acgcctttgc ggtggccagc
     1501 gacgcgcggc agcattcgct gcttggccca gcgaccattg ccgtccatga tgatggcgat
     1561 gtggcgcggc acggcgcgca cttccggaac ttcgcgagtc gagctggtaa acaaggttgc
     1621 gatcacaccg ccatcagatc ggcttccttc gcgtgcagca gcttgtcgac gtcggcgata
     1681 taccggtcgg ttagcttctg cacgtcgtcc tgcgcgcggc gctcgtcatc ctcggcaacc
     1741 gacttcgcct tgagcaaatc cttcagatgg ctgatcgcat cgcgacggat gttgcgaacg
     1801 gcgacgcgtg cattctccgc ttcccgatgc accaccttga tcagctcctt gcgccgctcc
     1861 tcggtaagcg ccggcatcgg cacccggatg aggtcgccgc tggtcgccgg attgagaccg
     1921 aggtcggaat cgcgaatcgc tttctcgacc gggtgaacca tctttttttc ccaaggctgc
     1981 acagtgatcg tatgcgcgtc ggccaaggtc acattcgcga cctggttgat cggcatcatc
     2041 gtgccgtagt agtcgacctt gatgtgatcg agcaggccgg tgtgtgcgcg gccggtcctg
     2101 accttgccca ggtcggtctt cagggcttcg accgattttt gcattttctg ctcggcggcc
     2161 ttctttacgt cagcgatcat tgggagctcc ttgccgaatc ggttgcacgc ggccagcgtc
     2221 ctgttgcgcg aatccgcgat cgcgcgcagc gtgcggtctt gttcagggac cgcacggacc
     2281 acagtgctag caatgcacca gcgtgccttc gtcctcgccc attaccacgc gcttgagcgc
     2341 accaggcgcg aagatgctga ataccttgag cagcatgttc tggtcgcggc agagcgcaag
     2401 cgcggtggcg tccatcacct tcaggttctt gacgatcgcc tcgtcgaaag tcagtgtcgg
     2461 atagcgccgc gccgactggt ccttgttcgg atccgcagtg tagacgccgt ccaccttggt
     2521 agccttgagc acgatgtcga cacccatctc gcggccgcgc aatgcggctg cggtatcggt
     2581 ggtgaagaaa gggttgccgg taccggcagc gaatatgacg atcttgtttt cttccagata
     2641 gcgcagagcc ttgccgcgga tgtacggctc ggcgacctgt tcgatcgaca acgccgactg
     2701 cacgcgcgac accaacccgg cgcggcgcat cgcgtcctgc agcgccagcg cgttcatcag
     2761 cgtggccagc atgcccatgt agtcggcggt ggcgcggtcc atgcccgccg ctcccggagc
     2821 cacgccgcgg aaaatgttgc cgccgccgat cacgaccgcg acctgcacgc ccagccggac
     2881 cacctcgcct atttcggcaa cgatccggtc gatgacctcg cgattgatgc catagggatc
     2941 gttccccatc agcgcttcgc cggaaagctt gagcaggatg cgcttgtacg ccggctcagt
     3001 gcgcggcagc ggcgccacgt tggacacagg cgcgatgttt tgcggtgtca tggcaggttc
     3061 gtcctccgca atgattgctc aggccttggc catcgccgct acttccgcgg cgaaatcatc
     3121 tttttttctt tcgatgccct cgccgacgac atagagcgtg aaaccccgga cggtcgcgcc
     3181 cttcgacttc agatactttt cgaccgtctg ctcggtgtca atcgcgaacg gctggccgag
     3241 caaggttacc tcggccaggt atttgttgat ccggccttcg accattttcg ccacgatctc
     3301 ggccggcttg ccgctatcgg ctgcttgcgc gctgtagatc gcgcgttcct tgtcgatcag
     3361 ctcggccgga acctgatcac gcgaaacgca caccggccgc acgttggcga cactcgcggc
     3421 gatatgcatc gctacgtcct tgccggtctt ctcgtcaccg ccctcgatct cgaccatcac
     3481 gccgatgcgg ccgccgtgga ggtattgcgc cagccgcgct ggcgtcgcga cgcgggagaa
     3541 gcgccgcacc gaaatgttct cgccgagctt ctgcaccagc gcctgccgcg ccgattcgac
     3601 cgtgccatcg cctagcggaa gtgcgagcaa cgcggcgacg ccagccggat tcttttcgac
     3661 gacgagcgca gccagtttgc ccgcgaacgc gatgaaatcg gcgttgcgcg cgacaaaatc
     3721 ggtctcgcag ttgagctcga gcagcgcg
//
LOCUS       DATANS010000778         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_38784, whole genome shotgun sequence.
ACCESSION   DATANS010000778 DATANS010000000
VERSION     DATANS010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38784"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VHT22_11630"
     CDS             <1..416
                     /locus_tag="VHT22_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138255.1"
                     /translation="PAWRALGWTPPITLGGIGVTHAAEVLWPTRGLPPPDGSVYPPPP
                     IALLPMRSVAVDATVQGDRALIVAFPYVPWMIPPQIEVSANGIARAPLARDVAAAVYA
                     CQECVGAAAVAWHLSITSSAPDRVDVVTIAPPRRE"
     gene            413..>1672
                     /locus_tag="VHT22_11635"
     CDS             413..>1672
                     /locus_tag="VHT22_11635"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138256.1"
                     /translation="MSAHVCAGAPSGWRSLARSLSMLCLVAMVAACSEEPTRPDLGRL
                     YRVGDVDTTPVIVIPGVFGSRLRDRVSGVEVWPGTSNEILFGEYRNIALDFDPVTLQV
                     RSDKLEAYGIAEMALGHDFYGQIIDTLRRFGGYVPGTPGTAAPQGERRYYIFAYDWRQ
                     DNVEAARGLDRLIEAIRRDYANPSLRVDIVAHSMGGLIARYYLRYGTVDVLDGSEQQV
                     TLYGTTRVRKLVLLGTPNMGSASSLHAFLIGEPVGLKRIPQEVLATLPSGYQLFPHPL
                     VTWLIDITGAPLDDELFDGATWRRYRWSIFDRAVEARIRAARGADADAYIAALQRYFD
                     YRLERARRFMWALSTPEPATPIRYVLFGGDCTLTPARLALEYEIETPVARLYPADIRR
                     PLAGVRYDELMLEPGDGTVTKPSLLARE"
BASE COUNT          243 a          576 c          564 g          289 t
ORIGIN      
        1 ggcccgcatg gcgcgcgctg ggctggacgc cgccgatcac tctcggcggg attggagtga
       61 cgcatgccgc agaggtgctg tggcccacgc gaggcctgcc gccgcccgac ggcagcgtct
      121 atcctccgcc gccgatcgcg ctgctgccga tgcgctccgt cgctgtcgat gcgaccgtgc
      181 agggcgatcg tgcgctgatc gtcgccttcc cctatgtgcc gtggatgatt ccgccgcaaa
      241 tcgaggtcag cgccaacggg attgcacgcg cgccgctggc gcgcgacgta gccgcggcgg
      301 tctatgcatg ccaggaatgc gttggcgccg cggcggtcgc gtggcacctg tcgatcacct
      361 cgtcggcacc cgatcgggtc gatgtcgtca cgatcgcgcc accgcgacgc gaatgagcgc
      421 tcatgtttgc gccggcgcac catccggatg gcgctcgctg gcgcgatccc tgtcgatgct
      481 gtgcctggtc gcgatggtcg ccgcctgcag cgaggagccg acgcgccccg acctgggccg
      541 gctctaccgg gtcggcgacg tcgatacgac gccggtcatc gtcatcccgg gcgtattcgg
      601 ctcgcggctg cgcgaccggg tcagcggcgt cgaagtctgg cccggcacgt cgaacgaaat
      661 cctgttcggc gagtatcgga atatcgccct cgacttcgac cccgtcacac tgcaggtccg
      721 gagcgacaag ctcgaagcct acggcattgc cgaaatggcg ctgggacacg acttctacgg
      781 ccagattatc gacaccttgc gccgcttcgg cggttatgtc cccggaacac ccggtacggc
      841 agcgccgcag ggcgagcgcc gctactacat ctttgcgtac gactggcggc aggacaacgt
      901 cgaagcggcg cgcgggctcg atcgcctgat cgaagcgatt cgtcgcgact acgccaatcc
      961 gtctttgcgg gtcgacatcg tggcgcacag catgggcggg ctgatcgcgc gctattacct
     1021 gcgctacggc accgtcgacg tgctcgacgg cagcgagcag caggttacgc tctacggcac
     1081 gacgcgcgtg cgcaagctgg tgctgctcgg aacgccgaac atgggctcgg cctcgtcgct
     1141 gcacgccttc ctgatcggcg agccggtcgg actcaagcgg attccgcagg aagtgctggc
     1201 gacgctgcct tccggctacc agctgttccc gcatccgctg gttacctggc tgatcgacat
     1261 caccggtgcg ccgctcgacg acgagctatt cgacggggcg acctggcgcc gctatcgctg
     1321 gtcgatcttc gatcgcgcgg tcgaggcgcg gatccgcgct gcgcgcggcg ccgacgccga
     1381 cgcgtatatc gcggcgcttc aacgctattt cgattaccgg ctcgaacgcg ctcgccggtt
     1441 catgtgggcg ctgtcgacgc cggagccggc tacgccgatc cgctatgtgc tgttcggcgg
     1501 cgattgcacg ctgacgccgg cgcgccttgc gctggagtac gaaatcgaaa ctccggtcgc
     1561 gcgcctgtat ccggccgaca tacgccgtcc gcttgcgggc gtgcgctacg acgagctgat
     1621 gctcgagccc ggggacggca ccgtgaccaa gccatcgctg ctggcgcgcg ag
//
LOCUS       DATANS010000779         1578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_375645, whole genome shotgun sequence.
ACCESSION   DATANS010000779 DATANS010000000
VERSION     DATANS010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375645"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VHT22_11640"
     CDS             complement(<1..576)
                     /locus_tag="VHT22_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX3138257.1"
                     /translation="MTFPSGTLLAQSALSGVFIGSMYGLLGMGLGLAWGLLHIINLAH
                     FGFAFLAAYLCYQLASVGGIDPLLTLALIIPLFFALGMAVQWLLSRFSVSPLNSLLAT
                     FGLTAIIEAGIQYFWTADFRKLESVYSGQKLRVGGLFVPVPELLTLVLALVLGFSIWA
                     AMRYTNLGRALRALAEDAPMAAAFGVNRRAHA"
     gene            complement(573..>1578)
                     /locus_tag="VHT22_11645"
     CDS             complement(573..>1578)
                     /locus_tag="VHT22_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3138258.1"
                     /translation="GLLGRPVEWVVKDDQSKPDLARTLYEQLITSDKVDLLMGPYATG
                     AILSAMGVAQRYNKVLVHHTFGIPSLAKYDKQFPAWSLGPDPANTVPTTVFTALAASP
                     KPPKTIAVVTSKFPSVHFMSLGAREVAKKRGLNEVLFLEWDFGNLDYGPIAARIKDAK
                     PDFLWVGALGLEGNQLLEAMNKIDYAPAEHFYMYPAPGPMSKSPLAKNALSMTMFEEH
                     PPFTNAAGAKEFIDLYHERAAKAGIPDTSVEVQAAASYTAWQILEAAVRGTNSIDDAK
                     MAAWLKANRVDTIQGKLRFDGPSNYGDDLMRVKQVQNGRWVTVYPKEYAAPGATLLVQ
                     "
BASE COUNT          285 a          520 c          512 g          261 t
ORIGIN      
        1 cgcatgcgcg cgccggttaa cgccgaaagc agccgccatc ggcgcgtcct cggccagcgc
       61 gcgcagcgcg cggccgaggt tcgtgtagcg catcgccgcc cagatggaga accccaggac
      121 cagcgccagc accagcgtca gcagttccgg caccggtacg aacagcccgc ccacgcgcag
      181 cttctgcccg gaatagacag actcgagctt gcggaaatca gcggtccaga agtactggat
      241 gccggcttcg atgattgcgg tcaggccgaa cgtcgctagc agcgaattga gcggcgacac
      301 cgaaaagcgc gacagcagcc attgcacggc catccccagc gcgaagaaca gggggatgat
      361 cagagcgagc gtcagaagcg gatcgatgcc accgacgctc gcgagctgat agcaaaggta
      421 agccgcaagg aacgcgaacc cgaagtgcgc gaggttgatg atgtgcagca gcccccacgc
      481 gaggccgagc cccatcccca gcagcccgta catgctgccg atgaagacgc ctgagagcgc
      541 cgactgcgcg agcagcgtcc cgctcgggaa cgtcattgca cgagtagtgt cgcgcccgga
      601 gccgcgtact ccttcgggta aacggtgacc cagcgcccgt tctgcacctg cttgacgcgc
      661 atcaggtcgt caccatagtt gctcggaccg tcgaagcgca gcttgccctg gatcgtgtcg
      721 acgcggttgg cctttagcca tgccgccatc ttcgcgtcgt cgatactgtt ggtgccgcgc
      781 accgccgcct cgaggatctg ccacgccgtg tacgaggccg cggcctgcac ctcgacgctg
      841 gtgtcgggga tgccggcctt cgccgcgcgc tcgtgataaa ggtcgatgaa ctccttcgcg
      901 cccgccgcat tggtaaaagg cggatgctct tcgaacatcg tcatcgacaa cgcgttcttc
      961 gcgagcggcg acttggacat cgggcccggg gccgggtaca tgtagaaatg ctcggccggg
     1021 gcgtagtcga tcttgttcat cgcctcgagc agctggttgc cctcgagtcc aagcgcgccg
     1081 acccagagga agtcgggctt ggcgtccttg atgcgcgcgg cgatcggacc gtagtccaga
     1141 ttgccgaagt cccattcgag gaacagcact tcgttgagtc cccgcttttt cgcgacctcg
     1201 cgcgcgccga gcgacatgaa gtgaaccgac gggaacttgc tcgtcactac ggcgatcgtc
     1261 ttcgggggct tgggcgacgc ggccagcgcg gtgaacaccg tggtcgggac cgtgttcgcc
     1321 ggatcgggcc cgagcgacca ggccgggaac tgcttgtcgt acttggccag cgacgggatg
     1381 ccgaacgtgt ggtgcaccag caccttgttg tagcgctgcg cgacgcccat cgccgacagt
     1441 attgcgcccg tcgcatacgg tcccatcagc aggtcgacct tgtccgacgt gatgagctgc
     1501 tcgtacagcg tgcgcgccag gtccggcttc gactggtcgt ccttgacgac ccattcgacc
     1561 ggccggccga gcaggccg
//
LOCUS       DATANS010000780         1799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_834591, whole genome shotgun sequence.
ACCESSION   DATANS010000780 DATANS010000000
VERSION     DATANS010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834591"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VHT22_11650"
     CDS             complement(<1..183)
                     /locus_tag="VHT22_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138259.1"
                     /translation="MLESAEIGHRVAKDVYAREEPRLRESLLLAQFELSRTRRGPVLV
                     IVSGVDGAGRGETANAL"
     gene            complement(197..>1799)
                     /locus_tag="VHT22_11655"
     CDS             complement(197..>1799)
                     /locus_tag="VHT22_11655"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX3138260.1"
                     /translation="TTWRWREKRTSSAQTWQRASGRTRFAQHFSMPTEVELKLSATPA
                     AWAAVRVHRALTEANPGRARSTMIVSRYYDTPAHELHRHGIALRLRRRGTRWLQTVKS
                     AGDAAAGMHRRAEYEWPLSRASLDPAKLAETPWAELFAKASGQLAAVFTTEVKRTDRA
                     LKFGDGTRATLSYDQGVVRAGRKRAPLCEIEIELVEGDARRLYELALALCADIPLTLA
                     HASKADQGYALARSRPAEPLRAGRVVLPPDIAAPHALAATAADCLGQMGGNAEAMRAG
                     RDGEFLHQLRVGVRRMRSLLKLASGLQSAAAIASLDVGLVGLSEVLGPARDWDVLADT
                     LAEIASNLDGDERRGFHRLRLRAAAKRRFHRAAAQSEAGSQRFTCSLLALGQFRIELE
                     LAAAGPPATAFARDALDKAERRLRKRGKRLGEADAAGRHKVRIAAKKLRYAAEFLASL
                     FRHGGAKDYIDALSGLQSALGHLNDMIAATRLIDELLAAARNDTELSRAAGIVRGWTA
                     AMSARELERLPKSWRQFAKAKPFWN"
BASE COUNT          271 a          627 c          606 g          295 t
ORIGIN      
        1 cagcgcgtta gccgtctccc cgcgcccggc gccgtcgacg ccggaaacga tgaccagcac
       61 cggtccgcgc cgggttctcg acagctcgaa ttgcgcgagc agcagcgatt cccgaaggcg
      121 gggttcctcg cgcgcgtata cgtccttcgc gacgcgatgg ccgatttctg cagattcgag
      181 catcgttggc tccggttcag ttccagaacg gcttggcctt ggcgaattgc cgccaggact
      241 tgggcaggcg ttccagctcg cgcgcgctca ttgccgcggt ccagccgcga acgatgcccg
      301 ccgcacgcga aagctcggtg tcattgcgcg cggctgccag gagctcgtcg atcagacgcg
      361 tcgcggcgat catatcgttc aggtgtccga gcgcgctctg caagccggac agcgcgtcga
      421 tgtagtcttt ggcgcctcca tgacgaaaca gcgacgccag aaactcggcg gcgtaacgca
      481 gttttttcgc ggcgatccgc accttgtggc ggcccgcggc gtccgcttcc ccaaggcgct
      541 tgccacgttt gcgcaatcgc cgctcggcct tgtcgagtgc atcgcgggcg aaggcggttg
      601 ccgggggccc ggcggcggca agctcgagct cgatacgaaa ctgtcccagc gcgagcagag
      661 aacaggtgaa gcgttgcgat cctgcttcgg actgcgctgc cgcgcgatga aaccggcgct
      721 tcgccgctgc gcgcaggcgc aggcgatgaa agccgcgccg ctcatcgccg tccaggttcg
      781 acgcgatctc ggccagtgtg tcagcgagga cgtcccaatc acgcgccgga ccgagtactt
      841 cgctcagccc gaccagaccg acatcgagcg atgcgatcgc ggccgcgctt tgcaagccac
      901 tcgcgagctt caacagcgag cgcatgcgtc ggacaccgac tctgagctga tgcaggaact
      961 cgccgtcgcg cccggcacgc atcgcttcgg cgttgccgcc catctggccg aggcaatcgg
     1021 ccgccgtcgc ggcgagcgcg tgcggcgccg cgatgtcggg cggcagcacg accctccccg
     1081 cgcgcaacgg ctcggccggc cgtgatcgcg ccagagcgta cccctggtcg gccttgctcg
     1141 catgcgcgag cgtcaacgga atgtccgcgc atagcgccag cgccagctcg taaaggcgcc
     1201 gggcatcgcc ctcgaccagc tcgatttcga tttcgcacag cggcgcgcgc ttgcggccgg
     1261 cacgaaccac gccctgatcg tacgatagcg tggcgcgcgt cccatcgccg aatttcagcg
     1321 cgcggtcggt acgcttgact tcggtggtga aaaccgccgc cagctgcccg gacgccttgg
     1381 caaacagctc ggcccagggc gtctccgcga gcttggccgg atcgaggctg gcgcgcgaca
     1441 gcggccattc gtattcggcg cgccgatgca ttccggcagc ggcgtcgccg gcgctcttga
     1501 ccgtctgcag ccagcgggtg ccgcgccgcc gcagccgcag cgcgatgccg tggcgatgca
     1561 actcgtgggc cggcgtgtcg taatagcggc tgacgatcat ggtcgaacga gcgcgaccgg
     1621 ggttcgcctc ggtcagcgcg cggtgcaccc gcactgcggc ccatgccgcc ggggtggcgg
     1681 aaagcttgag ttcgacctcg gtcggcatcg agaagtgttg cgcgaaccta gtgcggccgc
     1741 tggctcgctg ccaggtttgc gcgcttgatg tacgtttctc gcgccagcgc cacgtcgtc
//
LOCUS       DATANS010000781         1871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_165425, whole genome shotgun sequence.
ACCESSION   DATANS010000781 DATANS010000000
VERSION     DATANS010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165425"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VHT22_11660"
     CDS             <1..628
                     /locus_tag="VHT22_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX3138261.1"
                     /translation="AMYALFDNCNSLHTNAYDEALTTPTEESVRRAVAIQMIINRELG
                     LNKNQNPWAGSYIIDALTDLVEEAIYKEFEAISERGGVLGAMETMYQRSKIQEESLYY
                     ETKKHDGSLPIIGVNTFLSDSDSSDELKGAQLIRSTEEEKQDQVAAVRAFQARNADRS
                     ADALKKLQAVAASGGNVFEQLMEAVKCCSLGQISRALYQVGGQYRRNM"
     gene            732..1751
                     /locus_tag="VHT22_11665"
     CDS             732..1751
                     /locus_tag="VHT22_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX3138262.1"
                     /translation="MKAVGLYRNLPIDDPESLVDLDVPKPAPSDRELLVEVKAISVNP
                     VDYKIRARPDPNAPQPRILGWDASGVVREVGTGAQLFEPGDEVYYAGSIQKPGANSEF
                     HVVDERIVGHKPRSLGFAAAAALPLTTLTAWEGLFDRFGISSSGADRGKSVLLIGGAG
                     GVGSIAIQLAKQLAGLRVVATASRPESAQWVKELGADAVVDHTGDLAAQLQAAGTPEA
                     DYVFCLTDATPYFPRFAAITRPQGKLCLIVSMTEPVQLTSLMQKNVSIHWEFMFTRSL
                     FQTPDMQRQHEILEEAARLIDNGTLRTTLAESLGPINAANLRRAHQLLERGRTIGKLV
                     LEGFR"
BASE COUNT          374 a          593 c          585 g          319 t
ORIGIN      
        1 ggccatgtac gcgctgttcg acaactgcaa ctcgttgcac accaacgcct acgacgaggc
       61 actgaccacc ccgacggaag agagcgtgcg gcgcgcggtg gcgatccaga tgatcatcaa
      121 tcgcgagctc gggctgaaca agaaccagaa tccgtgggcg ggctcgtaca tcatcgatgc
      181 gctcaccgat ctggtcgagg aagcgatcta caaggaattc gaggcgatct ccgagcgcgg
      241 cggcgtgctc ggcgcgatgg agacgatgta ccagcgcagc aagatccagg aagagtcgct
      301 gtactacgaa accaagaagc acgacggctc gctgccgatc atcggcgtga atacgttcct
      361 ctcggacagc gattcctccg acgagttgaa gggcgcgcag ctcatccgct cgactgaaga
      421 ggagaagcag gatcaggtcg ccgccgtgcg cgcgttccag gcgcgcaatg ccgatcggtc
      481 ggctgatgcg ctcaagaaat tgcaggcggt cgctgcgtcg ggcggcaacg tgttcgagca
      541 gctgatggaa gcagtgaagt gctgctcgct cgggcagatc tcgcgggcgc tctatcaggt
      601 cggcgggcaa taccgaagga acatgtaaga agatttctcg ccaagacgcc aaggaagaaa
      661 agtctgaaaa ttcaatttcc ggaacttggc gtctaggcgt cttggcgaga gtctcatccg
      721 ggtcagctcg aatgaaagca gtaggtctct atcgcaacct tcccatcgac gaccctgagt
      781 cgctcgtcga tctcgacgtc ccgaaaccgg cgccgtccga tcgagagttg ctggtcgaag
      841 tcaaagccat ctccgtcaat ccggtcgact acaagatccg cgcgcgcccc gatcccaacg
      901 cgccgcagcc gcggatactc ggctgggatg cgtcgggcgt cgttcgcgaa gtcggcaccg
      961 gcgcgcagct gttcgagcca ggcgacgagg tctactacgc cggcagtatc cagaagccgg
     1021 gtgcgaacag cgaattccac gtcgtggacg agcgcatcgt cggccacaaa ccgcggtcgc
     1081 tcgggttcgc cgccgccgcg gccttgccgc tcactacgct caccgcgtgg gaagggctgt
     1141 tcgatcgctt cgggatttcc agctccggcg ccgaccgcgg caagagcgtg ctgctgatcg
     1201 gcggcgcggg cggcgtgggc tcgatcgcga ttcagctcgc gaagcagctc gcaggcctgc
     1261 gtgtggtcgc gaccgcatcg cgacctgaat cggcacagtg ggtcaaagag ctcggcgccg
     1321 acgccgtcgt cgatcacacc ggcgatctcg cggcacagct gcaggcagcg ggcacgcccg
     1381 aagccgacta cgttttctgc ctcaccgatg cgacgcctta cttcccgcgc tttgcagcga
     1441 tcacccggcc gcaaggcaaa ctgtgcctga tcgtcagcat gaccgagccg gtgcagctca
     1501 cttcgctgat gcagaagaac gtgtcgatcc actgggagtt catgttcacg cgctcgctgt
     1561 tccagacgcc cgacatgcag cgtcagcacg agatcctcga agaggcagcg cgcctcatcg
     1621 acaacggcac gttgcgcacg acccttgccg agagtctcgg ccccatcaat gcggcgaatc
     1681 tgcgtcgcgc ccaccagctg ctcgagcgcg gccgcacgat cggcaagctg gtgctcgaag
     1741 gattccgttg accgactccc gatgaaagca gagcacttct accagcgcct ggaccacggc
     1801 atcacggtgg tcgataccgg tttcgttcgc ccgggtttcg atgcgagttt cctgatcgtc
     1861 gaaaacggcc g
//
LOCUS       DATANS010000782         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_208891, whole genome shotgun sequence.
ACCESSION   DATANS010000782 DATANS010000000
VERSION     DATANS010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208891"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VHT22_11670"
     CDS             <1..787
                     /locus_tag="VHT22_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HEX3138263.1"
                     /translation="PARLLRDLGSVKAIFNLGAGIDSLAGSEALPAGVPVVRVEDAGM
                     AEQMSEYVVHAVLRQYREFDSYGEAQRAGLWRPLARRQKRGFGVGILGIGVLGTAVAA
                     ALLPFGFPLAGWSRTRKAVAGVDSYAGSAELDAFLARCQMLVCLLPLTRDTRGLLDRR
                     ALSQLPVGAHLVNVARGALIVDDDLLALLDQGRLGGATLDVFQDEPLPAAHPFWHHPR
                     ISITPHVSAVTLVEESVAQIAAKITRLEAGLPITGIVDPERDF"
     gene            792..>1586
                     /locus_tag="VHT22_11675"
     CDS             792..>1586
                     /locus_tag="VHT22_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX3138264.1"
                     /translation="MRSTGLANSSKLPRRVRIVDVAPRDGLQNEKAVVPTDVKVGLID
                     RLTDAGLPAIEATSFVSPRWVPQMADNAQVMAAIRRKPGVRYPVLTPNIKGFEAALAA
                     GADEVAVFVAASETFSQKNINCSISESLERARPVAAAAKTHGVRVRGYISCVLDCPYE
                     GEIAPSAVREVAAALQAMGCYEISLGDTIGTGTPGRARRLIATVAETVPVETLAGHFH
                     DTYGQAIANVYAAMEAGIATFDSSVAGLGGCPYAKGATGNVATEDLL"
BASE COUNT          255 a          512 c          544 g          275 t
ORIGIN      
        1 gccagcgcgt ctgctgcggg acttgggatc ggtcaaggcc atattcaatc ttggcgccgg
       61 catcgactcg ctcgccggga gcgaagcctt gccggcaggc gtccccgtcg tgcgggtaga
      121 agacgccggc atggcggagc agatgagcga atacgtcgtc catgcggtgc ttcgtcagta
      181 tcgcgaattc gattcttacg gcgaggcgca gcgtgcggga ctctggcgtc cgctcgcgcg
      241 acggcaaaag cgcggcttcg gcgtcggcat actcggcatc ggcgtgctgg gcaccgcggt
      301 cgcggcggcg ctgctgccgt tcggttttcc gctcgcgggc tggagccgga cgcgaaaagc
      361 cgtcgcgggt gtcgacagct atgccggcag cgccgagctc gacgcctttc tcgcgcgttg
      421 ccagatgctg gtgtgcctgt tgccgctgac acgcgatacc cgcggcctgc tcgatcgccg
      481 tgcgctctcg cagttgccgg tcggcgcgca tctggtcaac gttgctcgcg gtgcgctgat
      541 cgtcgatgat gatctcctcg cgctgctcga ccagggcagg ctcggcggtg cgacgctcga
      601 tgtctttcag gacgagcctt taccggccgc gcatcctttc tggcatcatc cgcgcatcag
      661 catcacaccc catgtctcgg cggtcacgct ggtcgaagag tcggtcgcgc agatcgccgc
      721 caaaataacc aggctcgagg ccggattgcc aatcaccggc atcgtcgacc ccgagcggga
      781 tttctaagcc gatgcgatcg acggggctgg caaatagcag caagctgccg cggcgggtgc
      841 gcatcgtcga tgtggcgccg cgcgacggct tgcagaacga gaaggcggtc gttccgaccg
      901 atgtcaaggt cggcctgatc gaccgcttga ccgatgccgg cctgccggcg atcgaggcga
      961 ccagcttcgt ttcaccccgc tgggtgccgc agatggccga caacgcgcag gtgatggcgg
     1021 cgattcggag gaagcccggc gtgcgctatc cggtgctgac gccgaacatc aagggcttcg
     1081 aggcggcgct cgccgcaggc gccgacgaag tggcggtatt cgttgccgca tcggaaacct
     1141 tttcgcaaaa gaacatcaat tgctcgatca gcgaaagcct cgagcgcgcg cgtccggtcg
     1201 ccgccgcggc caagacgcac ggcgtgcgcg tgcgcggcta catctcctgc gtgctcgact
     1261 gtccgtacga aggcgagatc gcgccgtctg cggtgcgcga ggtcgcggcc gcgctgcaag
     1321 cgatgggctg ctacgagatt tcgctcggcg atacgatagg caccgggacg ccggggaggg
     1381 cccgaagact gatcgcgacc gtcgccgaaa cggtcccggt cgaaaccctg gccggacatt
     1441 ttcacgatac ctatggccag gcgatcgcca atgtgtatgc cgcgatggaa gccggcatcg
     1501 ccacgttcga cagctcggtg gcgggcctcg gcggatgccc ttacgccaag ggcgcgaccg
     1561 gtaacgtcgc caccgaggac ctgctt
//
LOCUS       DATANS010000783         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_208902, whole genome shotgun sequence.
ACCESSION   DATANS010000783 DATANS010000000
VERSION     DATANS010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208902"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /locus_tag="VHT22_11680"
     CDS             complement(<1..1464)
                     /locus_tag="VHT22_11680"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX3138265.1"
                     /translation="MGILDLGRYKGIVFAITGFLVFIAIILAVNHGKAGQFSDDVAAV
                     KFLAEQREQPGAVNAAGIELAARLKNGQKIDEPLEALRKAAKSYDNAMSSLTNGGMIT
                     DSAGNVFVVPALTTDEAQGSLTKGNKLWNAYKAKLDPVLRFSGSPYAAPDAPATPAIK
                     HEAAVEQSTVPLSPRGRRLQAALQDLNTYGATSNTQLRDVMVELTSQVEAASRSRSGT
                     LRTVQVFGIVAAVLLLGAIFFYFARNMRKEEAVSNRQRKETADILRTVNEGLFLLDKD
                     LKVGSERSLALNQIFRREEFTGFTFDQLLKEIVPEKTLRTAMDYVALLWGDRVNEKLV
                     KTINPLNEVEVHFDNASGGFDTHFLEFDFNRVKAEGSLSHLLVTVNDVTKRVMLSREL
                     QESQEKAQAQLDLLLRILHVEPDSLTGFLTDADVSLKMVNSILKEPAREESAFRAKID
                     GIYRQVHAVKGEAAALGLKTVEQRAHAFEEALSDLKGR"
BASE COUNT          263 a          484 c          505 g          322 t
ORIGIN      
        1 gcgacccttg agatcgctga gcgcttcttc gaatgcgtgt gcgcgctgct cgacggtttt
       61 gagaccgagt gccgcggctt cgcccttgac ggcgtgcacc tgacggtaga tgccgtcgat
      121 cttcgcgcgg aacgccgact cttcgcgggc cggctccttg aggatcgagt tcaccatctt
      181 cagcgacacg tcggcgtcgg tgaggaagcc cgtgaggctg tccggctcga cgtgcaggat
      241 gcgcagcagc aggtcgagct gcgcctgcgc cttttcctgc gactcctgca actcgcgcga
      301 cagcatgacg cgcttggtga cgtcgttgac cgtcaccagc aggtgcgaca gcgagccttc
      361 cgctttcacg cggttgaagt cgaattcgag gaagtgcgtg tcgaaaccgc cggaggcgtt
      421 gtcgaaatgc acttcgactt cgttgagcgg gttgatcgtc ttgaccagct tctcgttgac
      481 gcggtcgccc cacagcagcg ccacgtagtc catcgcggtg cgcagcgtct tctcggggac
      541 gatctctttc agcagctggt cgaacgtgaa gccagtgaac tcttcgcggc ggaagatctg
      601 gttcagcgcg agcgagcgct cgctaccgac cttcagatct ttgtcgagca ggaacagacc
      661 ttcgttcacc gtgcgcagaa tatctgcagt ttccttgcgc tggcgattcg agacggcctc
      721 ttccttgcgc atgttgcgcg cgaagtagaa gaagatcgcg ccgagcagca gcacggcggc
      781 gacgatgccg aacacctgca cggtgcgcag agtgccggag cggctgcggc tcgcggcttc
      841 cacctgcgac gtcagctcga ccatcacgtc acgcagctgc gtgttcgacg tcgcgccgta
      901 agtattgagg tcttgcagcg ccgcttgcag gcggcgaccg cgcgggctca gcggcacggt
      961 gctctgttcg accgccgcct cgtgtttgat ggccggcgtt gccggcgcat ccggcgcagc
     1021 gtacggcgaa ccggagaagc gcagcaccgg gtcgagcttg gccttgtaag cgttccagag
     1081 tttgttgccc ttggtgagcg agccctgcgc ttcatccgtc gtgagcgccg gaacgacgaa
     1141 cacgttgcca gcgctgtcgg tgatcatgcc gccgttcgtg aggctgctca tcgcgttgtc
     1201 gtacgacttc gcggctttgc gcagcgcttc gagcggctcg tcgatcttct ggccgttctt
     1261 caggcgcgcg gcgagctcga tgcccgcggc gttgactgca ccgggctgct cgcgctgctc
     1321 ggcaagaaat ttgacggcgg cgacgtcgtc ggagaactga ccggccttgc cgtggttcac
     1381 cgcgagaatg atcgcgatga acaccaggaa gccggtgatc gcgaagacga ttcccttata
     1441 acgaccaaga tccagaatgc ccatgaacaa ttcccctgtg cgatgcagcg cgttcctgag
     1501 gcaggacgcg agcaggtgtc gccccggctt acaactatgg tgttatctga caataagcgt
     1561 tttaacagcg ctcg
//
LOCUS       DATANS010000784         1867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_122507, whole genome shotgun sequence.
ACCESSION   DATANS010000784 DATANS010000000
VERSION     DATANS010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122507"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VHT22_11685"
     CDS             complement(<1..382)
                     /locus_tag="VHT22_11685"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6597 domain-containing transcriptional
                     factor"
                     /protein_id="HEX3138266.1"
                     /translation="MLPRPRERLVMQMLRYRPAAPLSAFIDQFWYARGCDPDRKRQSA
                     LPTGSVDLTFNLAADDLRVFENATDRVGMVFDGAVVHGAQSKYFVLDARRDVHVIGVH
                     FRPGGGAPLLGVNAQELTDRHVALS"
     gene            474..>1867
                     /locus_tag="VHT22_11690"
     CDS             474..>1867
                     /locus_tag="VHT22_11690"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX3138267.1"
                     /translation="MSRWSLLCLCLLALPALAQAPGSSSIEQFFDTFTADWVRRDPDL
                     ATATRYFSGAEQDRLEQQLTPNTRAFELERVALAKRGLNELAKFDTAKLTESQRLSAQ
                     LMHWMLDSVVRGDEYSDYRFPLEQFGGANVELVETLTLRHPVATEKDASNYVKRLAQV
                     AQRMDESLVEAQRVATKGLIPPAFIIKTTLASMRTFRDVAPDQNPLVTVLAQKMGAIE
                     GMAPEQRTKLQAEATKLVGAQVYPAWDRAIKLLESLQPKATDDAGLWRFKGGDAAYKF
                     FLSRFTTTTLTPEEIHQIGLKQVASIEGQMDTILKQLGRTEGPVRERIEKLRNDLRYP
                     DPASDASRAAIMKDTNAFIEDALKRSQSLFDLQPKTKVIAQPFPRFREASAAANYNRA
                     PLDGSRPAIFQIPLRDQRMTKFGLRSLVYHETVPGHHFQIALEQENTSLPRFRQARVL
                     GGISALSEGWALYA"
BASE COUNT          358 a          621 c          596 g          292 t
ORIGIN      
        1 cggacagcgc aacgtgacga tcggtcagct cctgcgcatt cacgccgagc agcggcgcac
       61 cgccgccggg acgaaaatgc acgccgataa cgtgcacgtc gcggcgcgca tcgagcacga
      121 aatatttcga ttgcgcgcca tgcacgacgg cgccgtcgaa gaccatcccg acgcggtccg
      181 tggcgttctc gaacacccgc agatcgtcgg cggcaagatt gaatgtaagg tcgacactac
      241 cggtcggtaa cgcgctctgc cgcttgcgat ccgggtcgca gccgcgtgcg taccagaact
      301 ggtcgatgaa cgccgacagc ggtgctgctg gtctgtagcg gagcatctgc atgacgaggc
      361 gctcgcgggg tcgaggcagc atgatagtcc cgggttgctg aatacacgag ctttccgcac
      421 actcgcgcgc tcgacacaag aggcttggga gaacgacgat gcgagtgaac ctgatgagcc
      481 gctggtccct gctgtgtttg tgcctgctcg cgttacccgc gctcgcgcag gcaccgggca
      541 gctccagcat cgaacagttc ttcgacacgt tcaccgcaga ctgggtccgg cgcgatcccg
      601 acctcgccac cgccacgcgc tatttcagcg gcgccgagca ggatcggctg gagcagcagc
      661 tgacgccgaa tacgcgcgcc ttcgagctgg agcgcgtcgc gctggcaaag cgcggcttga
      721 acgagctcgc caaatttgac accgccaagc tgaccgagtc gcagcggctc tccgcgcaat
      781 tgatgcactg gatgctggac tcggtcgtgc gcggcgacga gtactccgat taccgcttcc
      841 cgctggagca gttcggcggc gcaaacgtgg agctggtgga gacgctgacg ctgcgccatc
      901 cggtcgccac ggaaaaggat gcgtcaaatt acgtcaagcg cctggcgcag gtcgcgcagc
      961 gcatggacga atcgctggtc gaggcgcagc gggtggccac caaaggactg attccccccg
     1021 cgttcatcat caagaccacc ctcgcatcca tgcgcacgtt ccgtgacgtc gcgccggacc
     1081 agaacccgct cgtcacggta ctggcgcaga agatgggcgc gatcgaaggc atggctcccg
     1141 agcagcgcac gaagctgcag gccgaggcga ccaagctcgt cggtgcgcag gtgtatccgg
     1201 cgtgggatcg cgcgatcaag ctgctcgaat cgctccagcc gaaggcgact gacgatgccg
     1261 gcctgtggcg cttcaagggc ggcgacgccg cgtacaagtt cttcctcagc cgcttcacga
     1321 ccacgacgct gacgccggag gagatccatc agatcggctt gaagcaggtc gcgagcatcg
     1381 aaggacagat ggacacgatc ctcaagcagc tcggccgcac cgagggcccg gtacgcgagc
     1441 gcatcgagaa gctgcgcaac gatctgcgtt atccggatcc cgcgtccgac gcgagccgcg
     1501 cggcgatcat gaaagacacc aacgcgttca tcgaggacgc actcaagcgc agccagtcgc
     1561 tgttcgatct gcagccgaag acgaaggtca tcgcgcagcc gttcccgcgc ttccgcgaag
     1621 ccagcgccgc ggccaactac aatcgcgcgc cgctcgacgg ctcgcgcccg gcgatcttcc
     1681 agatcccgtt gcgcgatcag cgcatgacga agttcggcct gcgctcactc gtgtatcacg
     1741 agacggtacc gggtcatcat ttccagatcg cgctcgagca ggagaacacc agcctgccgc
     1801 gcttccgcca ggcccgggtg ctcggcggca tctccgcgct gtccgaaggc tgggcgctgt
     1861 acgccga
//
LOCUS       DATANS010000785         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_122521, whole genome shotgun sequence.
ACCESSION   DATANS010000785 DATANS010000000
VERSION     DATANS010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122521"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VHT22_11695"
     CDS             complement(<1..186)
                     /locus_tag="VHT22_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3138268.1"
                     /translation="MTRANRFIRAALAAIVMLAGGPVIAQEAVRIGVVAEFSGPFADY
                     GQQIVAGMKTYLRQNGEV"
     gene            337..1365
                     /locus_tag="VHT22_11700"
     CDS             337..1365
                     /locus_tag="VHT22_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3138269.1"
                     /translation="MKPLGTLDELPREYVAELTRANLVPLWPSMRALLPPQAPRTATL
                     PAYWEWTAIRPLLLRAGELTPMEKAERRVLVLANPGRGLDNLQASAAIYLGMQLVLPG
                     ETAPSHRHTPNAARIVVEGQGAVTLVEGEACAMEPGDLILTPTGQWHEHRHTGKVPVI
                     WLDVLDLPLMVYLDVSYAVEGTSQTAHPPAYGYAAGGLAPVAHGQRAQGAYPLLRYEW
                     RRTRDALQALAGSSKAGAPVQVAYVNPETGGQCLNTLAFAALLLRAGEEVVLPRDSAA
                     RVFHAVEGNGEAQVNDTAIAFGHADTFCAPGLATVRLANRSSRAPLYLVSADESPLQR
                     KLGVYEQR"
     gene            1379..>2062
                     /locus_tag="VHT22_11705"
     CDS             1379..>2062
                     /locus_tag="VHT22_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX3138270.1"
                     /translation="MSVRPDRSAPVLVVGGGIGGMAVALALSQSGLAVELFEQSAQIG
                     EIGAGLQLGPNGFAAMDALGVGERARKRAVYTDELVMMDAIDGNEVARVPVGEAFRRR
                     FNNPYAVIHRADIHQSVYEEVQLASDIAVRTSARVVRVDIEEQRVRLVDANGGTHEGA
                     AVIGCDGVHSVVRERLIGDAHRVSGHVVYRAVVPRAEMPADLRWNAAAIWVGPNCHLV
                     HYPLRGGEQY"
BASE COUNT          324 a          692 c          705 g          341 t
ORIGIN      
        1 gacctcgccg ttctgcctga ggtacgtctt catgcccgcg acgatctgct ggccgtagtc
       61 ggcaaacggc ccggagaact cggcgacgac gccgatgcgc accgcctcct gggcgatgac
      121 ggggccgccg gccagcatga cgatcgcagc cagtgccgcc cgaatgaatc gattcgctcg
      181 cgtcaagggg ccctcctcgg cgagtgcgcc agcagccagc aggcatgggg cgggcactcg
      241 gtatgtatgc atattgaatg tatgcatacg aaatagtacg ctcgctgcat cgccgcgtgc
      301 aacggcccgc atgcgaagcc caggagattt ttccgcatga aacctctcgg cacccttgac
      361 gagctgcccc gcgagtacgt cgcggagctt acccgcgcca acctggtgcc gttgtggccc
      421 agcatgcgcg cgctgctgcc gccgcaggcg ccgcgtacgg cgaccctgcc tgcttactgg
      481 gagtggacgg cgatccggcc gctgctgttg cgggccggag agcttacgcc gatggaaaag
      541 gccgaacgcc gggtgcttgt gctcgccaat cccggtcgcg gtctcgacaa tctgcaggcg
      601 tcggccgcga tctacctcgg catgcagctg gtgctccccg gcgaaaccgc acccagccat
      661 aggcatacgc ccaatgccgc gcgcattgtc gtcgagggac aaggcgcggt gacgctggtc
      721 gaaggcgaag cctgcgcgat ggagcccggc gatctgatcc tgacccctac cggccagtgg
      781 cacgagcacc gtcacacagg caaggtgccg gtgatctggc tcgatgtgct cgatttgccc
      841 ttgatggtct acctcgacgt ttcgtatgcg gtcgaaggga cttcgcagac tgcgcacccg
      901 cccgcttacg gctacgccgc cggcggactg gcgcccgttg ctcatggcca gcgggcgcag
      961 ggagcgtacc cgctgctgcg ctacgaatgg cggcgcacgc gcgacgcatt gcaggcgctc
     1021 gccggctcga gcaaagccgg cgccccggtg caggtcgctt atgtgaatcc ggaaacgggc
     1081 ggccaatgcc tgaacacact ggccttcgcg gcgttgctgc ttcgcgcggg cgaggaagtc
     1141 gttctccccc gcgactcggc ggcgcgcgtc ttccatgcgg tcgagggcaa tggcgaggcg
     1201 caggtgaacg acaccgcgat tgcgttcggg catgccgata ctttctgtgc gccggggctc
     1261 gccacggtgc gcctcgcaaa ccgctccagc cgcgcgccgc tctatctggt aagtgccgac
     1321 gaatcgccgc tgcagcgcaa gctcggcgtg tacgagcagc gttgacgcga gcgccgcggt
     1381 gagtgtccgc cccgaccgat cggcaccggt actcgtcgtc ggcggcggca tcggaggcat
     1441 ggctgtcgcg ctggcgttga gccagagcgg gcttgccgtc gagctcttcg agcagtcggc
     1501 ccagatcggc gagatcggcg ccgggctgca gctcggtccc aacggcttcg cagcgatgga
     1561 cgccctcggc gtcggcgagc gagcgcgcaa gcgcgcggtg tacaccgatg agctcgtcat
     1621 gatggatgcg atcgacggca atgaggttgc gcgcgtgccc gtgggcgaag ctttccgccg
     1681 gcgcttcaac aatccgtacg cggtcatcca ccgggccgat atccaccagt cggtttacga
     1741 ggaggtccag ctcgcatcgg acatcgccgt ccgcacttcc gcgcgcgtcg tgcgggtaga
     1801 catcgaagag cagcgcgtgc ggctggtcga cgccaacggc ggcacccacg aaggcgcagc
     1861 ggtcatcggc tgcgacggtg tgcattcggt cgtgcgcgag cgcctgatcg gcgatgcgca
     1921 ccgcgtgtcg gggcatgtcg tctatcgtgc ggtcgtaccg cgcgccgaga tgcccgccga
     1981 tctgcgctgg aacgcagcgg cgatctgggt cggcccgaat tgccacctcg tccattaccc
     2041 gctgcgcggt ggcgagcaat ac
//
LOCUS       DATANS010000786         1879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_419455, whole genome shotgun sequence.
ACCESSION   DATANS010000786 DATANS010000000
VERSION     DATANS010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419455"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VHT22_11710"
     CDS             <1..284
                     /locus_tag="VHT22_11710"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX3138271.1"
                     /translation="LLPELPTLDESGLRGYQIVGWNGLFVPTGTPTAIVTKLHSAVVS
                     ALGLPDVKERLTIMGADGVGDTPQHFAAFIKAEIPKWAQVVKAAGLKIE"
     gene            281..1636
                     /locus_tag="VHT22_11715"
     CDS             281..1636
                     /locus_tag="VHT22_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX3138272.1"
                     /translation="MTDVTRQLARFVVASRWTELPREVRHEAKRALLNWLGCAFGGCN
                     DPAVDTALAGLREFAGPPQATVLGRRERLDILNAALVNAIGSNVLDFDDTHLRTVIHP
                     TVPVAAAITALAEHVPVTGAQFLHTFVLGVEAECRIGLAVSPEHYNAGWHITSTCGVF
                     GAAVACGKLLKLDEQQMTWALGIAATQAAGLTAVHGSMSKCFNMGHAARNGLTAALLA
                     QKGFTSSERGIEAPRGFAHVLSPRCDLNAVVDGLGHRWELLENAYKPYPCGIVGHPII
                     DGCLELRNAHGIAPDAIARIELRVHPLVMELMGNPVPRTGLEAKLSVHHCAAAAFVFG
                     EVGVKEFTDACANDPAVVALRTRVVPTVDAAMAADAADVVVALKDGKKQHAFVPHALG
                     SLERPLTDADLENKFRALAAWGFSTCNAYDVIELLWSFDNINDAASLARTTVPSAATA
                     V"
BASE COUNT          346 a          608 c          597 g          328 t
ORIGIN      
        1 cgctgctgcc ggagctgccg acactcgacg aatcaggcct gcgcggctat cagatcgtcg
       61 gctggaacgg attgttcgtc ccgaccggca caccgacggc gatcgtgacc aaactgcatt
      121 ccgcagtcgt cagcgcgctt gggctgcctg acgtgaaaga gcgcctgact atcatgggcg
      181 cggatggcgt cggcgacaca ccgcagcatt tcgccgcgtt catcaaagcg gaaatcccca
      241 agtgggcgca ggtcgtcaag gcggcaggcc tcaaaatcga atgacggacg tcacgcgtca
      301 gctcgcgcgc ttcgtcgtcg cttcgcgctg gaccgaattg ccgcgcgaag tgcggcacga
      361 ggcgaagcgc gcgcttctta actggctcgg ctgcgcgttc ggcggctgca acgatcccgc
      421 cgtcgatacc gcactggcgg gcctgcgcga attcgcgggt ccgccgcagg cgaccgtgct
      481 cggccgccgc gagcggctcg atatactcaa cgcggcgctc gtcaacgcga tcggctcgaa
      541 cgtgctcgac ttcgacgaca cgcatttgcg cacggtcatc catccgacgg tgccggtggc
      601 ggccgcgatt accgcactgg ccgaacatgt gcccgttaca ggtgcgcagt ttctgcacac
      661 gtttgtgctc ggcgttgaag ccgaatgccg gatcggcctt gcggtgtcgc ccgagcatta
      721 caacgccggc tggcatatca cgtcgacgtg cggcgtattc ggcgccgcgg tggcgtgcgg
      781 caagctgctg aagctcgacg agcagcagat gacatgggcg ctcggcatcg cagcaacgca
      841 ggccgccgga ctgaccgccg tgcacggcag catgagcaag tgcttcaaca tgggccatgc
      901 cgcgcgcaac ggtctgaccg ccgccctgct cgcgcagaag gggttcacca gttccgaacg
      961 cggcatcgaa gcgccgcgcg gttttgcgca cgtgctgtcg ccgcgctgcg acctgaacgc
     1021 ggtcgtcgac ggactcggcc accgctggga gctgttggag aacgcgtata aaccctatcc
     1081 gtgcggcatc gtcggacatc cgatcatcga cggctgcctc gaactgcgca acgcccacgg
     1141 catcgccccg gacgcgatcg cacgcattga attgcgcgtg cacccgctcg tcatggagct
     1201 gatgggcaac ccggtcccgc ggaccggtct cgaagcgaag ctgtcggtgc atcattgcgc
     1261 cgccgcggca ttcgttttcg gcgaggtcgg cgtcaaggag ttcaccgatg cctgcgcgaa
     1321 tgatcccgcg gttgtggccc tgcgcacgcg cgtcgtgcca accgtcgatg cggcgatggc
     1381 agcggatgcc gccgacgtcg tagtcgcgct caaggatgga aagaagcaac atgccttcgt
     1441 gccgcacgcg ctgggcagcc tggagcgccc gctcaccgac gcggatctcg aaaacaaatt
     1501 ccgcgcgctc gcggcgtggg gattttcgac ctgcaatgct tacgacgtga tcgagctgct
     1561 atggtcgttc gataacatca acgacgcggc ttcgcttgca cgcacgaccg tgccgtcggc
     1621 cgcgacggcc gtgtagtaat atcgcaatag aagaacgcga cccccatcct accttccccc
     1681 gtaaacggag gaaggggata ccggcatgcg ccggaatgac gaattaattt ttctgaaaat
     1741 tagtaagcgg agaatcccat gccctacgca gactcgaacg gcgtcaagct ctattacgaa
     1801 gaagccggcc gcggtgtgcc gatcgttttc gtgcacgaat tcgccgacga cctttatggc
     1861 tgggaagacc agatccgct
//
LOCUS       DATANS010000787         2820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_624394, whole genome shotgun sequence.
ACCESSION   DATANS010000787 DATANS010000000
VERSION     DATANS010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624394"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="VHT22_11720"
     CDS             complement(<1..1444)
                     /locus_tag="VHT22_11720"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HEX3138273.1"
                     /translation="MSVTTTSGDRTTRRPVPRDMARILGDCRDLAIHRLLLSFTSMLD
                     RVGDLLMARAERSDVREDQSVFLDAREALTRERANLMADFERHLRQLVDERIDGKGLA
                     KADFSTVDARKLTLVDTEAMDESVLSGNIVRVVENTCHEELRELNRGVGFLMGEPNLE
                     TAANPLAPTTIVAAFTEALHGIKGDRRIKLAILKELNQTSFGDIAAIYADVNRHLEGM
                     NVVPQLRPAAVQRGVGGHGGRGADGKTDGSAPPQGGGEVDLMAMLQRLVSSRLGQQGA
                     APMPAGLPPGGIQVPDAGPSPTAHEPGQALSMQALVSALGMMASSGAAQQAQPAVGPF
                     GGPRILATPELGDALSRLQHGDSNMNYGGMSFQLAGLQQDMHNVLRDLQESPLGSKAN
                     QLEAMTIELVAMLFDFIFETKDLPDSIKALIGRLQMPVLRAAMLDGAFFSKKSHPSRL
                     LVNALAHAGIGWSPTMGHEDPLYRKIEAIVH"
     gene            complement(1612..>2820)
                     /locus_tag="VHT22_11725"
     CDS             complement(1612..>2820)
                     /locus_tag="VHT22_11725"
                     /inference="COORDINATES: protein motif:HMM:NF023326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEX3138274.1"
                     /translation="KLSWYLKALPKGWRNRLTPPAEVVTAFLESTESDLSAAGAELAD
                     ALRVFLSARLGEAVPADIWDGAELPPHLRVNVRIVDASGKELAQSRDLSELRTQLGEA
                     AQLSFAAAGPEFERTGIRSWDFGDLPDTLTVERAGRRLTGYPALVVDGDSAALRLYDT
                     REAAEAATRIAVVRLIRQQLKDVLQRWEKNPPGFAAAALPLKTAIPTDALLADVLAAV
                     SDRAFIGEDPLPRSERAFADQVKRARTRLPAVVEGAFRMLAAIAADYHMLSQRLASLS
                     PSQARLGADVRAQRAALVYPGFFSATPWTQLTHLPRYLQALDKRLAKAAADPARDAKH
                     AQTVAALWDRYRARAEANRTAQRVEPALEGFRWLVEELKVSLFAQELRTPFPVSYKRL
                     EKAWAELIRR"
BASE COUNT          445 a          957 c          944 g          474 t
ORIGIN      
        1 ggtgcacgat tgcttcgatc ttccggtaca gcgggtcctc gtgccccatc gtcggcgacc
       61 agccgatgcc ggcgtgggcg agcgcattga ccagcaggcg cgacgggtgc gacttcttgg
      121 agaaaaacgc gccgtcgagc atggccgcgc gcagcaccgg catctgcagc cgtccgatca
      181 gggccttgat gctgtcgggc aggtccttgg tctcgaagat gaaatcgaac agcatcgcga
      241 ccagctcgat ggtcatcgct tcgagctgat tggctttgga tccgagcggc gactcctgca
      301 ggtcgcgcag cacgttgtgc atatcctgct gcagacccgc gagctggaag gacatgccgc
      361 cgtagttcat gttgctatcg ccgtgctgca agcgggacag cgcgtcgccc agctcggggg
      421 tcgccaggat gcgcggtccg ccgaacgggc cgacggccgg ctgcgcctgt tgtgctgcgc
      481 cgctgctggc catcatcccc agcgcgctga ccagcgcctg catcgatagc gcctgcccgg
      541 gctcgtgcgc tgtgggcgac ggtccggcat cgggcacctg gattccgccc ggcggaagtc
      601 cggcaggcat cggcgctgcg ccctgctggc ccagccggga actgaccagg cgctgcaaca
      661 tcgccatcag gtcgacctcg cccccgccct gcggcggggc ggatccatcg gttttgccgt
      721 cggcgccgcg ccctccgtga ccgccgactc ctctctgcac ggccgcgggt cgcagctgcg
      781 gcacgacgtt catgccctcg agatggcggt tgacgtccgc gtagatcgcg gcgatgtcgc
      841 cgaacgatgt ctggttgagc tccttcagga tcgcgagctt gatgcggcgg tcgcccttga
      901 ttccgtgcag cgcttcggta aacgcagcga cgatcgtcgt tggcgccagc ggattcgcgg
      961 cggtttccag gttgggctcg cccatcagaa agccgacgcc gcggttgagc tcacgcaact
     1021 cctcgtggca ggtgttctcg accacgcgca cgatgttgcc cgacaacacc gactcgtcca
     1081 tcgcctcggt gtccaccagc gtcagtttcc gcgcatcaac agtcgagaag tcggccttgg
     1141 ccagcccttt tccatcgatg cgctcgtcga ccagctggcg cagatggcgt tcgaagtcgg
     1201 ccatcaggtt ggcgcgctca cgagtcaatg cctcgcgtgc atcgagaaaa acggactgat
     1261 cctcgcgcac gtccgagcgc tcggcgcgcg ccatcagaag gtctccgacg cggtcgagca
     1321 tcgaagtgaa cgacagcagc agacgatgga tcgcgaggtc ccggcaatcg ccgagtatcc
     1381 tcgccatgtc tctgggcacc gggcgccggg tggtccggtc gccggaggtc gttgttacgc
     1441 tcacgcgttc ggtcctcgtt ggccgttcgg gtcctcaggc aacgtcaggg agtccagtcg
     1501 cgagccggcg ctgatcgcgc ctcccccgcc ccccgcctgg cttgagcccg caaaaacggc
     1561 aagttactgt aacacgcgaa gtttagggcc gccaatgcaa aaaaacgcca tctagcgacg
     1621 aataagctcc gcccaggcct tttccagacg tttgtatgac accgggaaag gcgtgcgtag
     1681 ctcttgggcg aagagcgaaa ctttcaattc ctccaccagc cagcggaacc cttcgagcgc
     1741 cggttcgacc cgctgcgccg tccgattggc ctcggcccgc gcccggtagc ggtcccagag
     1801 tgcggcgacc gtttgcgcgt gttttgcgtc ccgggccggg tcggccgcgg ccttggcgag
     1861 tcgtttgtcc agcgcctgca ggtagcgcgg caagtgggtg agctgggtcc acggcgtggc
     1921 cgaaaaaaat cccggataga ccagcgcggc gcgttgcgcc cgcacgtcgg cgcccaggcg
     1981 cgcttgcgac ggcgacagcg acgccagccg ctgcgacagc atgtggtaat cggcggcgat
     2041 cgcggccagc atccggaacg cgccctcgac gaccgcggga agccgcgtgc gagcgcgctt
     2101 gacctggtcg gcgaacgcgc gctccgagcg cggcagcggg tcctcgccga tgaacgcccg
     2161 gtcgctcacc gcagcgagca cgtcggcaag cagcgcatcg gtgggaatcg cagtcttgag
     2221 tggcagggcc gcggcagcga accccggcgg attcttctcc cagcgctgca agacatcctt
     2281 caactgctgg cggatcaggc gcacaacggc gatgcgcgtg gcagcttccg cggcttcgcg
     2341 cgtgtcgtac aggcgcagcg cagcgctgtc gccgtcgacg accagcgccg gatagccggt
     2401 cagccgccgg ccggcgcgct cgaccgtcag cgtgtccggc aaatcgccga aatcccagga
     2461 ccggatgccg gtgcgctcga actcgggccc ggcggcggca aagctgagtt gggctgcttc
     2521 gccgagctgc gtccgcagtt cggagagatc gcggctctgc gcgagttcct tgcccgacgc
     2581 gtcgacgatg cgcacattga cgcgtaggtg cggcggaagc tcggcgccgt cccagatatc
     2641 ggccggcact gcttcgccca ggcgcgcgct taagaacacg cgcagtgcgt cggccagctc
     2701 ggccccggca gcggacaggt cgctttcggt cgactcgaga aatgccgtga cgacctccgc
     2761 aggcggcgtc agccgattgc gccatccctt ggggagcgcc ttgaggtacc agctcaattt
//
LOCUS       DATANS010000788         1668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_209443, whole genome shotgun sequence.
ACCESSION   DATANS010000788 DATANS010000000
VERSION     DATANS010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209443"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /gene="lptC"
                     /locus_tag="VHT22_11730"
     CDS             complement(<1..444)
                     /gene="lptC"
                     /locus_tag="VHT22_11730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX3138275.1"
                     /translation="MKVRQPLLDRVVAWSPALLLGALVAMTYWLDAQVRAPAPKFDGS
                     GRHDPDITIQNFKAASLDDKGRIHQALIARAARHFPDDDSTDLEGPEITFNDPGKPRL
                     TVTADKASVSGDRDHVAFTGHVKGVRDASSDTAHDGPTTITSEYLL"
     gene            complement(441..971)
                     /locus_tag="VHT22_11735"
     CDS             complement(441..971)
                     /locus_tag="VHT22_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX3138276.1"
                     /translation="MTPADLDRRARRVRLLSCDVDGVLTDGKIYYAADGSQLKAFSTL
                     DGFGLKMLQESGVTVAWISGSRAPAVTRRAEDLSVAHVVLGADDKVAAWERLRKQLGI
                     DSQDCAHIGDDMPDLPVIMRCGLGVSVPHAPAALRGAAHYVTRADGGAGAVRELCELI
                     LAAKGALEPLLKAFAQ"
     gene            complement(968..>1668)
                     /locus_tag="VHT22_11740"
     CDS             complement(968..>1668)
                     /locus_tag="VHT22_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX3138277.1"
                     /translation="VVLLLSNSGETDELLVLLPHLKRQGARLIALTGNEQSSLAQAAD
                     IHLDASVDSEACPLGLAPTASTTAALALGDALALTVLDARGFSVEDFARSHPGGTLGR
                     RLLTRVRDVMRTGVALPMVGADASLADAVVEMSGKGMGMTAVVDDEQRVAGIFTDGDL
                     RRSLSRVRDFSAARVAELMTRNPRTIAADLLAIDCVELMETPPKVSQLLVVDGERRLV
                     GALHMHDLFRAKVV"
BASE COUNT          266 a          568 c          569 g          265 t
ORIGIN      
        1 cagcaggtat tcgctggtga tcgtcgtggg cccgtcatgt gcagtatccg acgacgcgtc
       61 gcgcacaccc ttaacgtggc cggtgaaagc cacgtggtcg cgatcgccgc tgacgctcgc
      121 cttgtcggcg gtgaccgtca agcgcggctt gcccggatcg ttgaacgtga tctcgggtcc
      181 ttcgaggtcg gtcgagtcat cgtccggaaa atgccgtgcc gctcgagcga tcagcgcctg
      241 atggatgcgg cctttgtcgt cgaggctggc cgctttgaag ttctggatgg ttatgtcggg
      301 gtcgtgcctt cccgagccgt cgaacttggg cgccggcgcg cgcacctgcg cgtcaagcca
      361 gtaggtcatg gcgaccagcg cgccgagcag cagcgcaggc gaccacgcga cgacgcggtc
      421 gagcagcggc tgacggacct tcattgcgcg aacgccttca gtaacggctc gagcgcgcct
      481 ttcgcggcga gaatgagctc gcatagctcg cgcacggcgc ccgccccgcc gtcggcgcga
      541 gtcacatagt gcgcagcgcc gcgcagcgcc gcgggggcgt gcggcaccga tacgcccagt
      601 ccgcagcgca tgatcaccgg cagatcgggc atgtcgtcgc cgatgtgcgc gcagtcctgg
      661 gagtcgatgc ccagctgctt gcgcagccgt tcccaggcgg cgactttgtc gtcggcaccg
      721 aggacgacgt gtgcgaccga aagatcctcg gcccgccggg tcacggccgg cgcgcggctg
      781 ccggaaatcc aggcgacggt cacgcccgac tcctgcagca tcttcaggcc gaagccgtcc
      841 aacgtcgaga atgccttgag ctggctgccg tcggctgcgt agtagatctt tccgtcagtc
      901 agcacgccgt cgacgtcgca ggacaaaagc cgcacgcggc gcgcccggcg gtcgaggtcg
      961 gcaggcgtca tacgaccttg gcccggaaga gatcgtgcat gtgcaacgcg ccgaccagcc
     1021 tgcgctcgcc atcgacgacg agcagctggc tgactttcgg cggcgtctcc atcagctcga
     1081 cgcagtctat cgccagcagg tcggccgcga tcgtgcgcgg gtttcgtgtc atcagctcgg
     1141 cgacccgcgc ggcgctgaag tcgcgcacgc gactcaagct gcgacgcaag tcgccgtcgg
     1201 tgaaaatgcc ggcgacgcgc tgctcgtcgt cgacgaccgc ggtcatgccc atgcccttgc
     1261 cgctcatctc gacgactgcg tcggcgagcg aggcatcggc tccgaccatc ggcagcgcga
     1321 cgccggtgcg catgacgtcg cgcacgcggg tcagcagccg tcgtcccagc gtgccgcccg
     1381 gatgcgaacg agcgaagtcc tcgaccgaaa agccgcgcgc gtcgaggacg gtcagcgcca
     1441 gcgcgtcgcc cagcgccagc gcggcagtgg tgctcgccgt cggcgcgagc ccgagcggac
     1501 aggcctcgct gtcgacgctg gcgtcgagat gaatgtcggc cgcctgagca agcgatgact
     1561 gctcgttgcc ggtaagcgcg atcagcctgg cgccttggcg cttcaagtgc ggaagcagca
     1621 ccaacagctc gtccgtttcg ccggaattcg acagcaacag cacgacgt
//
LOCUS       DATANS010000789         1898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_624735, whole genome shotgun sequence.
ACCESSION   DATANS010000789 DATANS010000000
VERSION     DATANS010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624735"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VHT22_11745"
     CDS             <1..616
                     /locus_tag="VHT22_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138278.1"
                     /translation="GDRGAVDSGNTLEYELDVDYSMMVQNLIDRIGPVVGDIRRVASQ
                     LGDPATGVQATLRRVAEAGRAVSKLGVDGGRLVGDARRVTNEIPARIDPVVKDLQRNL
                     AQVESLIKQLNADVPLMLGDTRKLLQSLTVSSENIQRLLTEDVPRVLHRSESVIQDAD
                     EIAGGVKRSWPVKNMLPQEIGERPVELDSADGAVHTAPRTAVPE"
     gene            613..1476
                     /locus_tag="VHT22_11750"
     CDS             613..1476
                     /locus_tag="VHT22_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138279.1"
                     /translation="MTAKGRRWHLVAPVLLAVLSGCASPPPPQSAADRRLEQANQAHE
                     RASAALTRGEYNTALRHYQATLDRSAAVEDQNESAISMLNIAAAQHRSGNYAAVRSTL
                     LALIAHEPPFAASYIGRAEARLSLVELQQNRVDEATLHAARAEQLCPAGACPWWLALQ
                     NIMTEIDLRRGDLGAADIRVRVTLAAAGRGTERREEANAWRLLGEIEARRGRLDEARR
                     ALLTALDIDGKLEVPERIALDLISLARLEAASGDREAGRRYARRAAAVAEAAKLAAVL
                     AQAHALLATAK"
     gene            1489..>1898
                     /locus_tag="VHT22_11755"
     CDS             1489..>1898
                     /locus_tag="VHT22_11755"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3138280.1"
                     /translation="MPAVKGAANRVAAYAYSAAASVWRLRMFANPMVRLVFLRQIYFT
                     AVRSLRLISIVAFVFGAAFVWHVTAVLGSDPRLFELVERVLMQNVAPLAAAIIVIGRS
                     ATAISSELAYMHCNGEVEALRHLRISAHDYLVEPR"
BASE COUNT          350 a          577 c          646 g          325 t
ORIGIN      
        1 cggcgaccgc ggggcggtgg atagcggaaa cacgctcgaa tacgagttgg acgtcgacta
       61 cagcatgatg gtccagaatc ttatcgaccg catcgggccc gtcgtcggcg acattcgcag
      121 ggtggcgtcg caattgggcg atcctgctac gggcgtgcag gcaacgctgc gccgtgtcgc
      181 cgaagcgggc cgcgcagtca gcaagttggg tgtcgacggc ggccgtcttg tcggcgatgc
      241 gcgccgcgtg actaacgaga tacctgcacg catagacccg gtggtgaagg atctgcagcg
      301 caatctggca caagtggagt cgctgataaa gcaactcaat gcggatgtgc ctttaatgct
      361 gggtgacacc cgcaagcttc tgcaaagcct tacggtatcc agcgagaaca tacagcgcct
      421 gctcacagaa gacgtgccgc gggtgctgca cagaagtgag tccgtaatac aggatgcgga
      481 cgagatcgcc ggaggcgtga agcgctcgtg gccggtcaag aacatgctgc cgcaggagat
      541 tggcgagagg ccggtcgagc tggacagcgc ggacggggca gtccacacag cgccgcgcac
      601 ggctgtgccg gaatgacggc caagggcaga cgttggcatc tggtggcgcc cgtgttgctg
      661 gcggtgctgt ccggctgtgc aagcccgccg ccgccccagt ccgctgcgga ccgccgcctc
      721 gaacaggcga atcaggcaca tgagcgtgcg agcgccgcat tgacgcgtgg cgagtacaac
      781 acggcattgc gccattacca ggctaccctc gatcgcagcg ctgcggtcga agatcagaac
      841 gagagtgcga tttccatgct caatatcgcc gcggcgcagc atcgtagcgg aaactatgcc
      901 gccgtgcgct cgacattgct cgcactgatt gcgcacgagc cgccattcgc cgcgagctac
      961 ataggccgcg ccgaagcgcg tctgtccctg gttgagctac agcaaaaccg cgtcgacgaa
     1021 gcaacgttgc atgccgcacg ggccgaacag ctttgccccg ccggggcgtg tccatggtgg
     1081 ctcgcgttgc aaaacatcat gacggagatc gacttgcggc gcggggatct gggcgccgcg
     1141 gacatcaggg tgcgcgtgac cctggctgcg gcaggccgcg gcacagagcg gcgggaggaa
     1201 gcaaacgcct ggcggctgct gggtgagatc gaggcccggc gaggacgcct cgacgaggcg
     1261 cgccgagctt tgctcaccgc gctcgacatc gatgggaaac tggaggttcc ggaacgcatc
     1321 gccctcgatc tcatctctct tgcgcgcctc gaggcggcgt ccggcgatcg cgaggcgggt
     1381 cgtcgctatg cgcggcgcgc ggcagcggtc gccgaagctg cgaagctcgc cgcggtgctc
     1441 gcgcaagcgc atgcgctgct cgctacggcg aagtagcgga tccgactgat gccggccgtg
     1501 aagggcgcag cgaacagagt cgcagcatat gcttactcag ctgcggccag cgtctggcgg
     1561 ctgcgcatgt ttgcgaatcc gatggtgagg ctggtctttc tgcgccagat ctactttacg
     1621 gcagtacgat ctttgcgcct gatctccatc gtcgcattcg tcttcggcgc cgcattcgtc
     1681 tggcatgtga ccgcggtgct gggcagcgat ccgcgcctct tcgaactggt ggagcgcgta
     1741 ttgatgcaga atgtcgcccc gctggcggca gccatcatcg tgatcggacg cagcgccacg
     1801 gcgataagct ctgaactcgc ctatatgcat tgcaacggag aggtggaggc attgcgccat
     1861 ctgcgcatat ctgcgcacga ctatctcgtg gaaccgcg
//
LOCUS       DATANS010000790         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_419791, whole genome shotgun sequence.
ACCESSION   DATANS010000790 DATANS010000000
VERSION     DATANS010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419791"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /gene="dgt"
                     /locus_tag="VHT22_11760"
     CDS             complement(<1..587)
                     /gene="dgt"
                     /locus_tag="VHT22_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HEX3138281.1"
                     /translation="MTADSPSLAPYAASEPASRGRAHAEPAPVLRGEYQRDRDRIIHS
                     TAFRRLVYKTQVFVNHEGDLYRTRLTHSIEVAQIARTIARALRLNETLAEAICLAHDL
                     GHTPFGHAGQDALNECMAGHGGFEHNLQSLRVVDALEERYPQFDGLNLCFETREGVLK
                     HCSRANAEKLEQAEPGGVGRRFLDRTQPSLEAQLC"
     gene            complement(604..1695)
                     /gene="aroB"
                     /locus_tag="VHT22_11765"
     CDS             complement(604..1695)
                     /gene="aroB"
                     /locus_tag="VHT22_11765"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008368039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX3138282.1"
                     /translation="MSAPVHERVDIALGERSYPILIGPRLLEDAQLLSTHITARSLLI
                     VTNETIAPLYLPKLQSALQGRRIATLILPDGEQHKTLESFSRILDSLVAERMNRDAAA
                     VALGGGVIGDMVGFAAACYQRGIDYVQVPTTLLAQVDSSVGGKTGVNHPRGKNMIGAF
                     HQPRCVLADTSTLQTLPAREYSAGLAEVVKYGFIYDIAFLQWIEANVEPLLAREDAVV
                     MHAVRRSCEIKADVVGQDEREHGLRAILNLGHTFGHAIETASGYGNWLHGEAVAAGMV
                     MAADMSTRLGWLQQAERDRVVQVLTRLGLPVEPPRIGAQRGRELMGMDKKVLGGRIRL
                     VLLQGLGKATVVDDYAAQALDATLQAHFG"
     gene            complement(1692..2228)
                     /gene="aroK"
                     /locus_tag="VHT22_11770"
     CDS             complement(1692..2228)
                     /gene="aroK"
                     /locus_tag="VHT22_11770"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HEX3138283.1"
                     /translation="MSSQAPRRNVFLIGPMGSGKTAVGKALARLLELTFYDSDVEIER
                     RTGVDIPYIFEKEGETGFREREREVIDSLTQLQDVVIATGGGAVLLPENRERLAARGR
                     VVYLRTGIRQQLERTKHGRQRPLLYTADPETRLRELMEVRAPLYESIASVTVATDGRH
                     VRIVADDILAQLRGLPQT"
BASE COUNT          369 a          750 c          773 g          399 t
ORIGIN      
        1 ttgcacagct gggcctcgag gctgggctgc gtgcggtcca ggaagcggcg cccgacgccc
       61 cccggctccg cctgctccag cttttcggcg ttggcgcgcg agcaatgctt cagcacgccc
      121 tcgcgcgtct cgaagcaaag attcaggccg tcgaactgcg gatagcgttc ctccagcgcg
      181 tcgaccacgc gcaggctttg caggttgtgc tcgaagccgc cgtggcccgc catgcattcg
      241 ttgagcgcat cctgccccgc gtggccgaag ggcgtgtggc ccaggtcatg ggccaggcaa
      301 atcgcttcgg cgagcgtttc gttcaggcgc agcgcccgcg cgatcgtgcg tgcgatctgc
      361 gcaacctcga tcgaatgagt caggcgcgtg cgatagagat cgccctcgtg attcacgaac
      421 acctgcgtct tgtagaccag gcgtcggaat gcggtcgaat ggatgatgcg atcccgatcg
      481 cgctgatact caccgcgcag caccggtgcc ggttcggcgt gcgcgcggcc tcgcgaggcc
      541 ggctcggagg cggcataagg tgcgagcgat ggggagtctg cggtcatgcg ctgcaggcga
      601 gtatcagccg aaatgtgcct gcagagtagc gtcgagcgcc tgcgcggcgt agtcgtcgac
      661 cacggtcgcc ttgccgaggc cctgcaacag caccaggcga atacgcccgc cgagcacttt
      721 cttgtccatt cccatgagct cgcgcccgcg ctgcgcgcca atgcgcggcg gttcgacggg
      781 cagacccagt cgcgtgagca cctgcacgac gcgatcacgc tcggcctgct gcagccagcc
      841 cagtcgcgtc gacatgtcgg cggccatcac cattccagcg gcgacggctt cgccatgcag
      901 ccagttgccg tagcccgagg cggtctcgat tgcgtggccg aaggtgtggc cgagattgag
      961 gatcgcacgt agaccgtgct cgcgctcgtc ctggccaacg acatctgctt tgatctcgca
     1021 ggagcggcgg accgcgtgca tcactacggc gtcctcgcgc gcgagcagcg gttcgacatt
     1081 cgcttcgatc cactgcagga acgcaatgtc gtagatgaaa ccgtacttga cgacctccgc
     1141 caggccggcg ctgtattcgc gcgccggcag cgtctgcaaa gtgctggtat ccgcgagcac
     1201 acagcgtggc tgatggaagg cgccgatcat gttcttgccg cgcggatgat tgacgccggt
     1261 cttgccgccc accgacgaat cgacttgtgc cagcagcgtc gtcggcacct gcacgtagtc
     1321 gatgccgcgc tgatagcagg cggccgcgaa gccgaccatg tcgccgatga cgccgccgcc
     1381 cagcgcgact gcagccgcgt cgcgattcat gcgctcagcg acgagcgagt cgaggatgcg
     1441 cgagaagctc tccagcgtct tgtgctgctc gccgtcgggc agaatgagcg tcgcgatgcg
     1501 gcggccttgc aaggcgctct gcagcttcgg caggtacagc ggcgcgatcg tttcgttcgt
     1561 gacgatcagc agactgcgcg cggtgatgtg cgtgctcaga agctgcgcgt cttccagcag
     1621 ccgcggcccg atcaagatcg gatagctgcg ctctcccaaa gcaatgtcga cgcgttcgtg
     1681 cactggcgcg ctcatgtctg gggcaggccc cgcagctgcg cgagaatgtc gtcggcgaca
     1741 atacgcacgt gtcgtccgtc ggtcgcgacg gtcacgctgg caatggattc gtagagcggg
     1801 gcgcgtactt ccatcagctc gcgcagtctg gtctcgggat ctgctgtgta gagcaacggg
     1861 cgctggcgac cgtgcttggt gcgctcgagc tgctggcgaa tgccggtgcg caggtaaacg
     1921 acgcggccgc gcgcggcgag ccgctcgcga ttctcgggca gcagcacggc gccgccgccg
     1981 gtggcgatga ctacgtcctg gagctgcgtc agagagtcga tcacttcgcg ctcgcgctcc
     2041 cggaatccgg tctcgccctc cttctcgaag atgtagggaa tgtcgacgcc ggtgcgccgc
     2101 tcgatttcga cgtcgctgtc gtagaaggtg agctccagca ggcgcgccag cgccttgccg
     2161 acggccgtct tgcccgagcc catcgggccg atcagaaaga cgttcctgcg aggggcctgc
     2221 gagctcatga cggcagggca ctataaacga aagcggccgc ccgaaggcgg ccgcggattc
     2281 gtcaccggca g
//
LOCUS       DATANS010000791         2215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_334604, whole genome shotgun sequence.
ACCESSION   DATANS010000791 DATANS010000000
VERSION     DATANS010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334604"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /gene="glpK"
                     /locus_tag="VHT22_11775"
     CDS             <1..1248
                     /gene="glpK"
                     /locus_tag="VHT22_11775"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX3138284.1"
                     /translation="TNQRETTLLWERASGKPVANAIVWQDRRTAATCDQLRAAGHEPL
                     FRSKTGLVLDAYFSGTKLKWLLDNVSGARERAMRGELAFGTVDSWLIWNLTGGAVHVT
                     DASNASRTLLFDIHRGDWDDELLALLDVPRAVLPTVVASSGVCADATIAGASVPIAGI
                     AGDQQAALFGQACLERGMAKNTYGTGCFLLLNTGAGAVPSSHHLISTIAWKRDGRIDY
                     ALEGSVFIAGAVVQWLRDGLKIIRNAAEIEALAASVPDNGGVYLVPAFAGLGAPHWDA
                     YARGAMFGLTRGSTSAHIARAALESIAYQSAEVLRAMQGDARLALAELRVDGGATANN
                     LLMQFQADLLGVPVVRPKVQETTALGAAYLAGLAVGYWKNTDDIRANWRVDRRFEPAM
                     TRERAAELTAGWDKAVKRAKGWA"
     gene            complement(1322..2149)
                     /gene="ugpQ"
                     /locus_tag="VHT22_11780"
     CDS             complement(1322..2149)
                     /gene="ugpQ"
                     /locus_tag="VHT22_11780"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX3138285.1"
                     /translation="MSGAGPLQDANRAPNGGSAAAGQPGGKRWGPTSAWPYPRYIAHR
                     GAGKLAPENTLAAMRVGYAHGYRMVEFDVKLSGDGVPFLLHDDTLDRTTDARGRADAL
                     TWAELAKLDAGSWHSAVYAGEVLPTLGAIAAWSIANGVACNIEIKPSPGRESATGAAV
                     ALDARALWRGAPIAPLMSSFSEAALAEAMAVAPELARAMLFEKVPPDWPERLARLQCV
                     ALDTDYREIDAALVADVHAAGYKMLCYTPNVPAQAAELVRWGVDGIITDAVDAIRPT"
BASE COUNT          352 a          757 c          773 g          333 t
ORIGIN      
        1 accaatcagc gcgagacgac gctgttgtgg gagcgcgcca gcggcaagcc ggtggccaac
       61 gcgatcgtct ggcaggaccg ccgcactgcc gccacctgcg accagctgcg cgccgcgggc
      121 cacgagcctc tgttcagaag caagaccgga ttggtgctcg acgcttattt ttccggcacc
      181 aagcttaaat ggctgctcga taacgtatcc ggcgcgagag agcgtgcgat gcgcggcgag
      241 ctcgcgttcg gcaccgtcga ctcctggctg atctggaacc tgaccggcgg cgcggtccac
      301 gtcaccgatg cgtcgaacgc cagccgcacg ctgctgttcg atatccatcg cggtgactgg
      361 gacgacgagt tgctggcctt gctcgacgtg ccgcgcgcgg tgctgccgac ggtcgtcgca
      421 tcgtcgggcg tgtgcgcgga tgcgacgatc gccggggcca gcgtgccgat cgcaggcatc
      481 gccggcgacc agcaggccgc actgttcggc caggcctgcc tggagcgcgg catggccaag
      541 aatacctacg gcaccggctg ctttctgctg ctcaacaccg gcgccggcgc cgtcccatcg
      601 tcccaccatc tgatatcgac aatcgcctgg aagcgcgatg gccgcatcga ctacgcgctc
      661 gagggcagcg tcttcatcgc gggagcggtc gtgcaatggc tgcgcgacgg gctcaagatc
      721 atccgcaacg cagccgaaat cgaggcgctg gcggcgagcg tgccggacaa cggcggcgtc
      781 tacctggtgc cggcgttcgc aggtctgggc gcgccgcact gggacgccta tgcgcgcggc
      841 gcaatgttcg gcctgacgcg cggctcgacc tccgcgcata ttgcgcgcgc ggcgctggag
      901 tcgatcgcct accagagcgc ggaagtgctg cgcgcgatgc agggcgacgc caggctcgcg
      961 ctcgccgagc ttcgcgtcga cggcggcgcc acggcgaaca acctgctgat gcagttccag
     1021 gcggaccttc tcggcgtgcc ggtcgtgcgt ccgaaagtgc aggagaccac cgccttgggc
     1081 gcggcgtatc tcgccggact cgccgtcggc tactggaaga acaccgacga catccgcgcg
     1141 aattggcggg tcgaccgccg cttcgagccg gcgatgacgc gcgagcgcgc agccgagttg
     1201 acggcgggat gggacaaggc agtgaagcgg gctaaaggct gggcgtagcg aaccggcttg
     1261 ttaagacgca actaaagacg caataagcgc aacggtcgtg taacgcgccg tcgccgccgt
     1321 ctcaagtcgg ccggatcgcg tcgaccgcgt cggtgatgat gccgtcgacg ccccagcgca
     1381 ccagctcggc cgcctgcgcg ggaacgttgg gcgtgtagca aagcatcttg taaccggcgg
     1441 catggacgtc ggcgaccaga gccgcatcga tctcgcgata atcggtgtcg agcgccacgc
     1501 attgcaggcg cgccaggcgc tccggccagt cgggtgggac cttctcgaac agcatcgccc
     1561 gcgccaactc cggtgcgacg gccattgcct cggccagcgc ggcttcggaa aacgacgaca
     1621 tcagcggcgc gatcggcgcg ccgcgccaca gcgcgcgtgc gtccagcgca accgcggcgc
     1681 cggtcgcgct ctcgcggccg gggctcggct tgatctcgat attgcacgcg acgccgttgg
     1741 caatcgacca cgcggcgatc gcgcccagcg tcggcaacac ttctcccgcg tacacggcag
     1801 aatgccagct cccggcatcg agcttggcga gctccgccca ggtcagggcg tcggcacggc
     1861 cgcgtgcgtc ggtcgtgcgg tcgagcgtat cgtcgtgcag cagaaacggc acgccgtcgc
     1921 cggacagctt gacgtcgaac tcgaccatcc gatagccatg cgcgtagccg acgcgcatcg
     1981 cggcaagcgt gttctccggc gccagcttgc cggcgccgcg gtgggcgata tagcggggat
     2041 acggccaggc gctcgtggga ccccaacgct tgccgccggg ttggccggcc gctgcgctgc
     2101 ccccgttggg ggcgcgattc gcgtcttgga gcggtccggc gccgctcatg ggcccggcgt
     2161 cacaacggca gccgcgcgcc gctttcggcg tcgaacaggt aaatgcggtc gcgat
//
LOCUS       DATANS010000792         1894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_585814, whole genome shotgun sequence.
ACCESSION   DATANS010000792 DATANS010000000
VERSION     DATANS010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585814"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="VHT22_11785"
     CDS             complement(<1..1253)
                     /locus_tag="VHT22_11785"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HEX3138286.1"
                     /translation="MAQRSDGDSLAERSSPRRRAAIVAIAVCASAFAIAVLAAPVDDL
                     RTAVEAGDSAAAYARHCAMSIDAPERPPEFDLWCGVAAVDIGRSGEGVLALERYVLQF
                     PDDARARLELARAYFYAGDDVRSREEFEAVARSQPPPDVQAGIDRYLAALTDREARYR
                     GRRTAFVELGGGYDSNANAGVAQATIGLPVLGPVTVDAFGVQKESAFGWLAAGAEIRH
                     PLSAGLTVNASVYGGGTFYASASEFNLANYGAAAGASYRADRNEYSLAYAHGEIALDG
                     SRYRWADGIVLEWRRQISELSSFALAPQYARIAYAGENAARDADLSAFSASYRQVWLR
                     RWQPVLNATVFYGDEHNREGRGDLGRGIAGASVDVTVSPSPFWALNAGLAYAQSDYDA
                     PIPLLAVTRRDRNLGVGLGAIYLVNR"
     gene            complement(1330..>1894)
                     /locus_tag="VHT22_11790"
     CDS             complement(1330..>1894)
                     /locus_tag="VHT22_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEX3138287.1"
                     /translation="KHGKALALAATAAMEAHDPAKALTYWHRLAAQLPPGSEDATQVA
                     AVIAELGGRAPDAVNTMPAPGPRASAAGNGGTVSGHVAVSPGLAAKVAPTDTVFIFAR
                     APTGPRMPLAAMRIPASELPKDFALDDAMSMAAGAKLSSASEVIVEARLSKTGNALPQ
                     PGDLFGKSGPVKPGATGLRITIDQVVP"
BASE COUNT          300 a          649 c          647 g          298 t
ORIGIN      
        1 cggttgacca ggtagatcgc gccgaggccc acgcccaggt tgcggtcgcg ccgcgtgacg
       61 gcgagcagcg ggatcggcgc gtcgtagtcg ctttgcgcgt aggcgaggcc ggcgttcagc
      121 gcccagaagg gcgatggcga cacggtgacg tcgacgcttg cgccggcgat gccgcgaccg
      181 agatcgccgc ggccttcacg gttgtgctcg tcaccgtaga acacagtcgc gttgagcacc
      241 ggctgccagc ggcgcagcca tacttgccga tagctggcgc tgaacgcgct caagtcggca
      301 tcgcgtgcgg cgttctcgcc ggcgtaagcg atgcgcgcgt attgcggcgc aagcgcgaac
      361 gaagacagct ccgagatctg tcggcgccac tccaagacga tgccatcggc ccagcgatag
      421 cgcgatccgt cgagcgcgat ctcgccgtga gcatacgcca gagagtactc gttgcgatcg
      481 gcccgatagc tcgcgccagc cgccgcgccg taattcgcca gattgaattc cgaggctgac
      541 gcgtagaacg tgccgccgcc gtagacgctg gcattgaccg tcaatccggc ggacagagga
      601 tggcgaatct cggcacctgc cgccagccat ccgaacgccg actccttttg gacgccgaaa
      661 gcatcgacgg tgaccggacc gagcaccggc aacccgatgg tggcttgcgc aacgccggcg
      721 ttggcgttgc tgtcgtagcc gcctccgagt tcgacgaacg ccgtgcgccg cccgcgatag
      781 cgggcctcgc gatcggtcag cgccgcaaga tagcgatcga tgccggcctg cacgtctggt
      841 ggcggctggc ttcgggccac tgcctcgaat tcttcgcgcg agcggacgtc gtcgccggcg
      901 tagaaataag cgcgcgccag ctcaagacgg gcgcgcgcgt cgtcgggaaa ttgcagcacg
      961 taacgctcga gggccaggac gccttcgcct gagcggccga tgtcgacggc cgcgacgccg
     1021 caccacagat cgaattccgg cgggcgctcg ggggcgtcga tcgacattgc gcaatggcgg
     1081 gcgtacgcgg cggcggagtc gccggcttcg accgcggtgc gcaagtcgtc gaccggcgcg
     1141 gcgaggacgg caatcgcgaa ggcgctagcg cacactgcga tcgcaacgat cgccgcacgc
     1201 cggcgcgggc tcgaacgctc cgccagggag tcgccgtccg aacgctgtgc catgcccgcg
     1261 ccgcccctcc cgaatttggc tggcggcgat catagcacgg caccgtgatg cttccggcgg
     1321 aacgactgcc tacggtacga cctgatctat tgtgatgcgc aagcccgttg cgccaggctt
     1381 gaccggcccg cttttgccga acaggtcacc gggctgcgga agcgcattac cggttttcga
     1441 caggcgcgct tcgacgatca cttcgctcgc actcgagagc ttcgcccccg cggccatgct
     1501 catcgcatcg tccagcgcga agtccttcgg caattcgctg gccggtatgc gcatcgcggc
     1561 gagcggcatg cgcggtccgg tcggagcgcg cgcgaaaatg aaaacggtat cggtaggtgc
     1621 gacttttgcc gccaggcccg ggctcactgc gacatggccg ctgaccgtgc cgccgttgcc
     1681 tgcggcgctg gcgcgcggcc ccggtgctgg catcgtgttc acggcatccg gcgctcggcc
     1741 acccagctcc gcaatcacgg ccgcaacctg cgttgcgtcc tcggagcccg ggggcagctg
     1801 cgcggcaagc cggtgccagt aggtgagcgc cttggcagga tcgtgtgcct ccattgccgc
     1861 cgtcgccgcc aaggccagcg ccttcccgtg tttc
//
LOCUS       DATANS010000793         3118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_544026, whole genome shotgun sequence.
ACCESSION   DATANS010000793 DATANS010000000
VERSION     DATANS010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544026"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VHT22_11795"
     CDS             <1..942
                     /locus_tag="VHT22_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017423176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX3138288.1"
                     /translation="RDPRLVGITPAMREGSGLVRFSQEHAGRYFDVGIAEQHAVTFAA
                     GLACEGMKPVVAIYSTFLQRAYDQLIHDVALQNLPVMFAIDRAGLVGADGPTHAGSFD
                     FCYLRCLPNMTVMAPADEGECRNMLYTAYMLDTPSAVRYPRGAGPGVAVNKEMRALPI
                     GKGELRRETGRRTQRIALLAFGSMLGPALAVAEQIDATVANMRFVKPLDAELCVALAR
                     THESLVTIEENVVAGGAGSAVAEALAARGIAVPLLQLGLPDAFIDQGDPASQLAHCGL
                     DAKGIAASIAARFGQPSGQSEDVDVAQPAIVRPAVRQ"
     gene            985..1782
                     /gene="folE2"
                     /locus_tag="VHT22_11800"
     CDS             985..1782
                     /gene="folE2"
                     /locus_tag="VHT22_11800"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HEX3138289.1"
                     /translation="MNRPETPLIPDVQSHPDARKLPIDRVGIRNLRYPIRFVDGTDPP
                     QSTIAVCSAYVALPADRKGTHMSRLVAAIDELAEPLSLAALPAFLGALLVRLEAQAGR
                     VDIAFPWFMRKSAPVTGAQSMMDYEVRLVADAAPGKCSVEATIVVPVMSLCPSSKAIA
                     DYGAHNQRSHLTLTVRPLPGAEITLAELIALAEAEASCELYGLLKRADEKFVTERAYD
                     RPRFVEDLVRGIAARLVDDARLVGFSIAAENFESIHNHSAYAEIVRG"
     gene            complement(1845..2342)
                     /locus_tag="VHT22_11805"
     CDS             complement(1845..2342)
                     /locus_tag="VHT22_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138290.1"
                     /translation="MRFLVLCVSFCGIAYPVAGHAQAIHKCLAPDGIAYQSMPCASDA
                     GDVVIASLASVRAEPTSRAEAPTAAPARREREANSANSKRVFWRTSIAIGMSDDEVLN
                     LPAWGKPSAITRSKANRVWYEEWIYRWRMGGTSRLSFANGRLTGVETAAMVEPAETQV
                     SYGPH"
     gene            complement(2441..3085)
                     /locus_tag="VHT22_11810"
     CDS             complement(2441..3085)
                     /locus_tag="VHT22_11810"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3138291.1"
                     /translation="MATRLEDGPSSNRLAWILDPQRSGLSLVMSAIGCHAQHLACFTS
                     GLAIARVAADARALVPGRKVVDYTSALMEFEGGARGTLTVTQAAAGGENDIRLRVYGE
                     KGMLDWSHRDLSYLRLALQGEPARVIGRGDPGLPPQIIALGRAPRGHPEGLREAFANI
                     YREVAQERMARALGEASPDAPYPRIEEGAHTMAFIEACIESQRRGGWVAVEKSG"
BASE COUNT          509 a         1060 c         1029 g          520 t
ORIGIN      
        1 cgcgacccgc gactggtcgg catcacgccg gcaatgcgtg aaggatcggg tctggtgcgc
       61 ttttcgcagg agcatgccgg tcgctatttc gatgtcggca ttgccgagca gcacgcggtg
      121 acgttcgcgg ccggactcgc ctgcgaaggc atgaagcccg tggtcgcgat ttactcgacg
      181 ttcctgcagc gagcctacga ccagctgatc cacgacgttg cgctgcaaaa cctgccggtg
      241 atgttcgcca tcgatcgcgc cgggctggtc ggcgccgatg gtccgacgca tgccggcagc
      301 ttcgacttct gctatctgcg ctgcctgccg aatatgacgg tgatggcgcc cgccgacgag
      361 ggcgaatgcc gcaacatgct ctacacggca tatatgctgg acacgccgag cgccgtgcgt
      421 tacccgcgag gcgccggacc gggcgtcgct gtcaacaagg aaatgcgcgc gctgcccatc
      481 ggcaagggcg agttgcgccg cgagaccggg cggcggacgc aacgcatcgc gctgctcgcg
      541 ttcggcagca tgctcggacc ggcccttgcc gtggccgagc aaatcgacgc caccgtcgcc
      601 aatatgcgct tcgtcaagcc gctcgacgcc gagctttgcg tggcgctggc ccgcacccac
      661 gaaagcctgg tcacgatcga ggaaaacgtc gtcgccggcg gcgccggcag cgccgtcgcc
      721 gaagcgcttg ccgcgcgcgg catcgcggtg ccgctgttgc aactcggact gccggatgcc
      781 ttcatcgatc agggcgatcc cgcatcgcag ctcgcgcact gcggtcttga cgccaagggc
      841 atcgctgcat cgatcgcggc ccgtttcggc cagccgtccg gccaaagcga ggacgttgac
      901 gttgcgcagc cggcgatcgt ccggcctgct gtgagacaat gacgggctcg tcgtaacgcg
      961 cctcgtccca cgcgaaccgc tgcaatgaat cgccccgaaa cgccgttgat cccggatgtc
     1021 cagtcgcatc ccgacgcgcg gaagctgccg atcgatcgcg tcggcataag aaatttgcgc
     1081 tatccgattc gctttgtcga cgggaccgac ccgccgcaat cgacgattgc cgtttgcagc
     1141 gcttatgtcg cgctgccggc ggatcgcaaa ggcacgcata tgtcgcgcct ggtcgcggcg
     1201 atcgacgagc tcgccgagcc gctgtcgctg gcggcgcttc cggccttcct cggggcgttg
     1261 ctggtccggc tggaagcgca ggctggccga gtcgacatcg cgtttccgtg gttcatgcgc
     1321 aagagcgccc cggtgaccgg cgcgcaaagc atgatggact acgaagtgag gctggtcgcc
     1381 gacgcggcgc cgggcaagtg ttctgtcgaa gcgacgatcg tcgtgccggt gatgagcctg
     1441 tgtcccagct cgaaggcgat tgccgactat ggcgcgcaca accagcggtc gcacctgacg
     1501 ctgaccgtgc gcccgttgcc aggcgccgag atcacgctgg cggagttgat cgcgctggcc
     1561 gaagccgagg cctcatgcga gctttacggc ctgctcaagc gagccgacga gaagttcgtc
     1621 accgagcgcg cctacgaccg gccgcggttc gtcgaggatc tcgttcgcgg catcgccgct
     1681 cgcttggtcg acgacgcccg gctggtcggc ttcagcatcg ccgcggaaaa tttcgagtcg
     1741 atccacaacc attcggcgta cgccgaaata gttcgcggct gaagcctagc ccgcaaaccg
     1801 gtgcgacgcg caagcaggga aggcgttttt taagagcggc gcggctagtg gggcccgtag
     1861 ctgacctggg tttccgccgg ttcgaccatc gcagcggttt ccaccccagt caacctgcca
     1921 ttggcaaacg acagccggct ggtcccaccc atcctccagc gatagatcca ctcctcgtac
     1981 cagacccggt tcgccttgct gcgtgtgatc gcgctcggct tgccccaggc cggcagattc
     2041 aggacctcat catcggacat gccgatagca atgctcgtgc gccagaagac gcgcttggaa
     2101 ttggccgagt tggcctcccg ctctcgcctt gcaggcgccg cggtgggcgc ttcggcgcgc
     2161 gaggtgggct cggcgcgcac gctggccagg ctggctatca cgacgtcacc cgcgtcggac
     2221 gcgcaaggca tcgactggta agcgataccg tcgggtgcca ggcacttgtg gatagcttgc
     2281 gcgtgtccgg caaccggata cgcgatgccg caaaagctca cgcacaggac caggaaacgc
     2341 atgacggcac ccggagggac gaccacggtg cacagcgtgc ccgccgggtc acgggaccgc
     2401 catcagccgc ggtctgatct tgttgtagga atccttccgg tcaaccggat ttttcgaccg
     2461 ccacccagcc gccgcgccgt tgcgactcga tgcaggcttc gatgaacgcc atcgtgtgcg
     2521 cgccttcctc gatccgcggg tagggcgcgt cggggctagc ctcgcccagc gcgcgggcca
     2581 tgcgctcctg cgccacctcc cggtagatat tggcaaaggc ctcccgcaag ccctccggat
     2641 ggccgcgcgg cgcgcggccc agggcgatga tttgtggggg caagccgggg tcgccgcggc
     2701 cgatcacgcg cgccggctcg ccctgcagcg ccaggcgcag gtagctcaag tcgcggtgcg
     2761 accagtcgag catccccttt tccccataga cgcgaagccg gatgtcgttc tcgccgcccg
     2821 cagcggcctg ggtcaccgtc agcgtgcccc gtgcgccgcc ttcgaactcc atcagcgccg
     2881 aggtgtaatc gacgaccttg cggccgggca ccagcgcgcg ggcgtcagca gcgacgcgtg
     2941 cgatcgcgag gccggaggtg aagcaggcca gatgctgcgc atggcagccg atcgcgctca
     3001 tcaccagcga gagaccactg cgctgcgggt cgaggatcca ggcgagccgg ttgctcgacg
     3061 gaccatcctc gaggcgcgtc gccaatccac tctgaatgta ttcgacctgg accaggcg
//
LOCUS       DATANS010000794         1845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_247176, whole genome shotgun sequence.
ACCESSION   DATANS010000794 DATANS010000000
VERSION     DATANS010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247176"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VHT22_11815"
     CDS             complement(<1..1005)
                     /locus_tag="VHT22_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HEX3138292.1"
                     /translation="MPRDPGFLAALPRLSSIAAAPRESRVLTSYDATLAWSALILLAL
                     GLVMVYSASIATAEALAYTGYRPWYFLVRHAAFVAIGLVAALFAFQIPLKIWQRLAPY
                     LFIAGAALLIAVLIPGIGKSVNGSKRWLSLYIVNLQPSELMKLAVVLYAASYAVRKAA
                     FLHAEQPLKQTLLRGFLPLLAVMVLVSALLLHEPDFGACVVIVAIAFGILFLGGLDWR
                     LLIGLGFLLPVTLWILIWIAPYRLQRFTGFLDPWADPLGKGYQLSHSLIAFGRGEWFG
                     VGLGASVEKLLYLPEAHTDFLLAVIAEELGFVGVALVIGLFAWLLIRAYGIGRQAARL
                     E"
     gene            complement(998..>1845)
                     /gene="murD"
                     /locus_tag="VHT22_11820"
     CDS             complement(998..>1845)
                     /gene="murD"
                     /locus_tag="VHT22_11820"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX3138293.1"
                     /translation="SSLKSTAATVLNVSANHLDRYAGIDAYARAKARIFAGAGEQILN
                     RDDFRSLSMRLPGHIVQTFGAGVPEAELDWGLVQRSGAPWLARGGALLLAASDLALVG
                     RHNALNALAALALTSTVAKIDGKVVAALAAFRGLPHRMERVAEIGGVLFVNDSKGTTV
                     AATQAALEGLGRPAVLIAGGDGKGQDFTPLKADVVKYCRAVILLGRDAPVIAAALSGV
                     DAAIEFAPALEVAVARAIARARPGDAVLLSPACASLDMFRDYVERGDRFKAAVAAHER
                     EVAHA"
BASE COUNT          342 a          627 c          635 g          241 t
ORIGIN      
        1 ctcgaggcgc gccgcttgcc ggccgatgcc gtaggcgcgt atcagcaacc atgcgaacag
       61 gccgatcacc agcgcgacac cgacgaaacc gagctcttcg gcgatgactg ccagcaggaa
      121 gtcggtgtgc gcttcgggca gatagagcag cttttccacg ctggccccga gcccgacgcc
      181 gaaccattcg ccccggccga aggcgatcag cgaatgcgac agctggtagc ccttgcccaa
      241 cgggtccgcc cacggatcca gaaagccggt aaagcgctgc agccgatagg gagcgatcca
      301 gatcaggatc cacaaggtga cgggcaatag aaacccgagg ccgatcagga gccgccagtc
      361 gaggccgccc aggaagagaa tgccgaacgc gatggcgacg atcaccacgc aggcgccgaa
      421 gtccggttcg tgcagcaaca gcgcgctgac cagcaccatc accgcgagca gcggcagaaa
      481 gccgcgcaac agcgtctgct tcagcggctg ctcggcgtgc aggaacgccg ctttgcgcac
      541 cgcgtagctc gccgcataaa ggacgacggc gagcttcatc agctcggacg gctgcaggtt
      601 gacgatgtag agcgacagcc agcgcttgct gccgttgacg ctcttgccga tgccgggaat
      661 aagcaccgcg atcagcagcg ccgcgccggc gatgaacagg tatggtgcga gcctctgcca
      721 gatcttgagc gggatctgga acgcgaacag cgcggcgacg aggccgatcg cgacgaacgc
      781 cgcatggcgc accagaaaat accagggccg gtagccggtg tatgccagcg cttccgcggt
      841 cgcgatcgac gccgaataga ccatgaccag gccgagggcc agcagtatca gcgccgacca
      901 ggccagcgtc gcgtcgtagg acgtcagcac acggctctcg cgcggcgccg cagcgatcga
      961 cgacagccgg ggcagcgcgg caaggaagcc cggatctcta ggcatgggcc acctcgcgct
     1021 cgtgcgcggc caccgccgcc ttgaagcgat cgccgcgctc gacgtaatcg cggaacatgt
     1081 cgaggctcgc gcaagccggc gacaacagca ccgcatcgcc gggccgcgcc cgcgcgatcg
     1141 cgcgcgcaac ggcgacttcg agcgccggcg cgaactcgat cgccgcgtcg acgccggaca
     1201 gcgccgcggc gatcaccggc gcgtcgcgtc cgagcaggat caccgcgcgg caatatttga
     1261 cgacgtcggc tttgagggga gtgaagtcct ggcctttgcc atcgccgccg gcgatcagca
     1321 ccgccggccg gcccaggccc tcgagcgcgg cctgcgtcgc ggcgaccgtc gtgcccttcg
     1381 agtcgttgac gaacaggacg ccgccgattt cggcgacgcg ctccatccgg tgcggcaggc
     1441 cgcgaaacgc ggcgagcgcg gcgaccacct tgccgtcgat cttggcgacc gtcgaggtga
     1501 gcgccagtgc cgcgagcgca ttgagtgcgt tgtgtcgtcc gaccagcgcc aggtcgctgg
     1561 cggcaagcag cagcgcgccg ccgcgcgcga gccagggcgc cccgctgcgc tgtaccaggc
     1621 cccagtcgag ttcggcctcg ggaacgcccg cgccgaacgt ctgcacgatg tggccgggaa
     1681 gccgcatcga gagcgagcgg aagtcatcgc ggttcaagat ttgctcgccg gcgccggcga
     1741 atatccgcgc cttggcgcgc gcgtaggcgt cgatcccggc gtaacggtcg aggtgattgg
     1801 cgctgacgtt gagcacggtc gctgcggtgg atttgaggct cgatg
//
LOCUS       DATANS010000795         1911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_754924, whole genome shotgun sequence.
ACCESSION   DATANS010000795 DATANS010000000
VERSION     DATANS010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754924"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..>1911
                     /locus_tag="VHT22_11825"
     CDS             295..>1911
                     /locus_tag="VHT22_11825"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HEX3138294.1"
                     /translation="MPITRSFRMDAWIPALLFAVGAGLAVFAGLTIFSWHAPEPVPMA
                     AGFDRIQYDAAWAIAFASAALALHVVGATRSARLFALLTLALGGVRLAAWVVPDGIPL
                     RPILANPWLPLADGSYNDMGLLTALIAVVIGAALLLLLRPGKSRRPWHAITVALLTSI
                     ALALSLLMLFAAWSGSTVASESLQLTGGERATGAVYTVLAATMLAYAMLTSGGERKAL
                     RRSAPLIVWLAVFACALVLWRALTVEESRFIQRSTALVAADARGQIQRDLYARIDIVE
                     RLADRSLAYADREAWKRDAQGLLKGVAAVDAVALSGADYVVEQAVPSSAEPFVGFDPR
                     IDPLRRAAIDLAVSTRRSTLSPFVDLKMGGEGLVLYVPVFQGGELRAIISASLARTGW
                     PKSILDQPFPEHYIEIVQNGRVVRTVAPDGTRAANKWSQELPLVVQNAHWTLRATPTQ
                     NLVASGASSLPIAALALGTIMATLLALSTYLFQTARANARNLKRTNERLVSDIARRYH
                     VEQELRESENRTSLIINAVKDCAIYMLDTEG"
BASE COUNT          297 a          634 c          609 g          371 t
ORIGIN      
        1 gacgacgtcc gccccgtccg ggcaaggctc ctcgctgagc tgtcgccgca atcggtcgca
       61 tcgagttcac caccgcttgc gatccgcgca acgcggcatt gacgcccttg tcgtgcgcgc
      121 aaatgacgcg gcgacgcgca cgtcgacgat gtgcgcgttg acgcgcacct tgtttcgacg
      181 ctataagctc ttcgctcgct gaatcgggag cccgaccgca ttccaccgcc ccgctgcgtc
      241 gggatcagct aacatccatt ctttgcgctt gccttgcggc gtgcgcaaga ccctatgccg
      301 atcacccgtt ccttccgcat ggatgcctgg attccggcgt tgctgttcgc cgtcggcgcc
      361 gggctcgccg tcttcgccgg tttgacgatc ttcagctggc acgcgccaga acccgtgccg
      421 atggctgcgg gtttcgatcg gattcagtac gacgccgcgt gggcgatcgc cttcgccagc
      481 gcggcattgg cgctgcacgt tgtcggcgca acacgaagcg ctcgcctgtt tgcgctgctt
      541 accttagcgc tgggcggcgt aaggctcgcc gcctgggttg tcccggacgg cattccgctg
      601 cgtccgatac tcgccaatcc ctggctgccg ctcgcggatg gttcctacaa tgacatgggg
      661 ctgctaaccg cgctgatcgc agtcgttatc ggcgctgccc tgctgctgtt gcttcgaccg
      721 gggaaaagcc ggaggccgtg gcacgcgatc acggtcgcgt tgcttacttc gatcgcgctg
      781 gcgctttcac tgctgatgct gtttgccgca tggagcggca gcacggtcgc gtccgagtcg
      841 ctgcagctga ccggtggcga gcgggcgacg ggcgcggtct acacggtgct cgccgccacg
      901 atgctcgcct acgcgatgct gaccagcggc ggcgagcgga aggcgttgcg ccgctcggcg
      961 ccgcttatcg tctggcttgc cgtttttgcg tgcgcacttg ttctctggcg cgcactgacc
     1021 gtggaggaat cgcgcttcat ccagcgcagc accgcgcttg tcgcagcgga cgcacgcggc
     1081 cagatccagc gcgatctgta cgcccgcatc gacatcgttg agcggctggc cgatcgttcg
     1141 ctggcgtatg ccgatcggga agcgtggaaa cgcgacgccc agggcctgct gaaaggcgtc
     1201 gcagcggtcg atgcggtggc gctgagcggc gccgattacg tcgtcgagca ggcggttcct
     1261 tccagcgccg agccgttcgt cggcttcgac ccacgcatcg atccgttgcg acgcgcagct
     1321 atcgatctgg cggtcagcac gcgtcgaagc acgctgagcc cctttgtcga cctgaagatg
     1381 ggaggggagg gtctcgtgct ctacgttccc gtattccagg gcggcgagtt gcgcgcgatc
     1441 atctcggcgt cactggcgcg taccggatgg ccgaagtcga tcctcgacca gccctttccc
     1501 gagcattaca tcgagatcgt gcagaacgga cgagtcgtgc gcaccgtcgc tcccgacggc
     1561 accagggcgg ccaacaaatg gtcgcaggag cttcccctgg tcgtgcagaa tgcgcactgg
     1621 acgctgcgcg cgacgccgac gcagaatctg gtcgcctccg gcgcatcgtc gttgccgatc
     1681 gccgcattgg cgctcggtac gatcatggcc acgctgctcg cgctttcgac ctaccttttc
     1741 cagactgccc gcgccaatgc aagaaatctc aagcgcacca acgagcggct ggtcagcgat
     1801 attgcacggc gctatcacgt cgagcaggaa ttgcgcgaaa gcgaaaaccg cacgagcctg
     1861 atcatcaacg cggtcaagga ttgtgcgata tacatgctcg acaccgaagg a
//
LOCUS       DATANS010000796         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_123150, whole genome shotgun sequence.
ACCESSION   DATANS010000796 DATANS010000000
VERSION     DATANS010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123150"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VHT22_11830"
     CDS             complement(<1..432)
                     /locus_tag="VHT22_11830"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX3138295.1"
                     /translation="MRCGVDEAGRGPLAGPVFAAAVILGDAHRIKGLADSKMLSAVKR
                     EALAVQIRRSAVAWAVASASVEEIDALNILQATLLAMRRAVESLALAPTEVLVDGLYC
                     PRITHPARAVVRGDALIPEISAASILAKTSRDALMLGLHAQY"
     gene            complement(434..1603)
                     /gene="lpxB"
                     /locus_tag="VHT22_11835"
     CDS             complement(434..1603)
                     /gene="lpxB"
                     /locus_tag="VHT22_11835"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX3138296.1"
                     /translation="MASSAEPAGRPRTIAIVAGEASGDLLGSQLILALREHLPALNVV
                     GIGGPRMEAAGMDVWFPLEKLAVRGYFEVLRHYFEIVGIRRKLRRRLLKIRPDVFIGI
                     DAPDFNLDLERGLKARGIPTVHYVSPAIWMWRRERLGKIKRAVSRMLTLFPFEEDIYR
                     DAGVDVAFVGHPLADMLDHIPRDDAARDQLRLPPASPVVALLPGSRQGELKYMADLFI
                     RTAKLIHARLPHAVFLVPLTTRETRVMFETALYHNDAEELPLTIMFGHAQDAMAAADV
                     VLVASGTATLEAALLCKPMVITYKMPPATYWLLQGKGYLPYYGLPNILAREFVVPEFI
                     QDDATPQNLSQALLNLLADVATRDRIAARFAAMSASLHRGAARQAALAVLPMLPD"
BASE COUNT          293 a          552 c          562 g          285 t
ORIGIN      
        1 atattgcgcg tgcaaaccga gcatcagcgc gtcgcgcgat gtcttggcca gtatcgacgc
       61 cgcggatatt tcgggtatca acgcgtcgcc acgcacgacc gcgcgcgccg ggtgcgtaat
      121 ccgcgggcaa tagaggccgt caacgagcac ttcggtcggc gccagcgcga gcgattcgac
      181 ggcccgtcgc attgcgagca gcgtcgcctg cagaatgttc aaagcgtcga tttcttcgac
      241 ggaagcgctg gctaccgccc acgccactgc cgagcgccgg atctgcaccg ccagtgcctc
      301 acgtttcact gcgctcagca ttttcgaatc ggcgagccct ttgatgcgat gcgcatcgcc
      361 gaggatcacc gctgcagcga agaccggacc cgccagcggg ccgcgcccgg cttcgtcgac
      421 gccgcaccgc atgtcagtcc ggcagcatcg gcagtacggc cagagcggcc tggcgggccg
      481 cgccgcgatg gaggctggcg ctcatggccg cgaagcgggc agcgatccgg tcgcgcgtcg
      541 ccacgtcggc gagcaggttg agcagcgcct ggctcaggtt ttgcggcgtc gcgtcgtcct
      601 gaatgaactc cggcaccacg aattcgcgcg cgaggatgtt gggcaggccg tagtacggca
      661 gatagccctt gccttgcagg agccagtacg tggccggcgg catcttgtag gtgatgacca
      721 tgggcttgca cagcagcgcc gcctcgagcg tcgcggtccc cgatgccacc agcacgacgt
      781 cggccgccgc catcgcgtcc tgcgcatggc cgaacatgat ggtcagcggc aattcttcgg
      841 cgtcgttgtg gtacagcgcg gtttcgaaca ttacgcgcgt ttcgcgcgtc gtcaacggca
      901 ccaggaatac cgcgtgaggc agtcgcgcat gaatcagctt tgcggtccgg ataaacaggt
      961 ccgccatgta tttcaactcg ccctggcggc tgccgggcag gagcgcgacc accggcgacg
     1021 ccggcggtaa tcgcagttgg tcgcgcgcgg cgtcgtcgcg cggaatgtgg tcgagcatgt
     1081 ccgcgagcgg atggccgaca aacgcgacgt ccacgcccgc gtcgcgatag atgtcttcct
     1141 cgaacgggaa cagcgtcagc atgcggctga cggctcgctt gattttcccg agccgctcgc
     1201 ggcgccacat ccagatcgcc gggctcacat aatggaccgt cggaatgccg cgcgccttca
     1261 gcccgcgctc gaggtcgaga ttgaaatccg gcgcgtcgat gccgataaag acatcgggcc
     1321 ggatcttgag caggcggcgg cgcagcttgc ggcgtattcc gacgatttcg aaatagtgcc
     1381 taagcacctc gaaatagccg cgcaccgcga gcttttcgag cgggaaccac acgtccatgc
     1441 cggcggcttc catgcgcgga ccgccgatgc cgacgacgtt gagcgcgggc aggtgctcgc
     1501 gcaaggcgag gatcagctgg ctgccgagca ggtcgcccga cgcttcgccc gcgacgatgg
     1561 caatggtgcg cgggcgtccc gcaggctcag cggatgatgc cacgcgtcga ctgggcgaga
     1621 aaatccagca atacctgcaa ttcggggcag tggcctacgt gggtcgcaat ctcctgcttc
     1681 gcgtcttcaa gt
//
LOCUS       DATANS010000797         3003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_754979, whole genome shotgun sequence.
ACCESSION   DATANS010000797 DATANS010000000
VERSION     DATANS010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754979"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VHT22_11840"
     CDS             complement(<1..662)
                     /locus_tag="VHT22_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX3138297.1"
                     /translation="MIKVRKGQAPPPHSRVEFGKRFRSAYFDPAFRGEQAALDRVEAI
                     AWNGYHEDRKAPVTRKAGPEFADPDYDISVEWLETRERVRAAAAKQTDPDTASRVLLI
                     CGAPRNDGSCPGEMSKTYRLTKLAGETLSKAGIETDLLDLSLITSDYRRHIHPCKGCV
                     STAMPLCHWPCSCYPNHAFGQTNDWMAEIYERWTAAHGVLIVTPVHWYQVPSVLKLMI
                     DRL"
     gene            complement(875..2134)
                     /gene="hemL"
                     /locus_tag="VHT22_11845"
     CDS             complement(875..2134)
                     /gene="hemL"
                     /locus_tag="VHT22_11845"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX3138298.1"
                     /translation="MTRNEQLFARAQKTIPAGVNSPVRAFRSVGGTPRFFTRGEGAYL
                     WDADGKRYIDYVGSWGPAILGHAHPETVRAVQETARHGLSFGAPTELEIEMAEELCRL
                     LPSLEMVRLVSSGTEATMTALRLARGYTGRSKIIKFEGCYHGHGDSLLVKAGSGALTF
                     GQPSSAGVPASIAGETLVLTYNDLDDVARAFAEHPDAIAGIIVEPVAGNMNLIAPKPG
                     FLAGLREICDRHGAVLIFDEVMTGFRLHLQGAQGLYGVAPDLTTLGKVIGGGMPVGAF
                     GGKRAIMEKIAPLGPVYQAGTLSGNPVAVAAGLATLKAIQSPGFHQRLAERTRALTDG
                     LTAAAARAGVAFSAQSIGGMFGIYFSATVPDSFAAVMACDKEAFNRFFHRMLDAGVYL
                     APSAFEAGFVSAAHTGADIDATVELAW"
     gene            complement(2235..2885)
                     /gene="thiE"
                     /locus_tag="VHT22_11850"
     CDS             complement(2235..2885)
                     /gene="thiE"
                     /locus_tag="VHT22_11850"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX3138299.1"
                     /translation="MAAAGSRTVPRPKLAGLYALTPDLADTEALLARTDRALSGGASA
                     IQYRNKAASAELRLQQAASLRALCSARGAIFIVNDDVELARAVGADGVHLGRDDASVA
                     AARAGLGPAAIVGVSCYDELARAEAAIADGGDYIAFGSFYPSLVKPNAVRPSLRLIAE
                     AKARWPGVGVVAIGGINAANATPLIAAGADAVAVISALYDAPDVLLAARELVACFG"
BASE COUNT          502 a         1016 c          989 g          496 t
ORIGIN      
        1 agccggtcga tcatcagctt gaggacgctg gggacctggt accagtgcac cggcgtcaca
       61 atcagcacgc cgtgcgccgc cgtccaccgt tcgtagatct ccgccatcca gtcgttggtt
      121 tggccgaacg cgtggttcgg atagcaactg cagggccaat ggcacagcgg cattgccgtc
      181 gatacgcaac ccttgcatgg atggatgtgc cggcggtaat ccgacgtgat caggctcaga
      241 tcgagcagat ccgtttcgat gcccgctttg gagagcgtct cgcccgcgag ttttgtcagc
      301 ctgtaggtct tcgacatttc gccgggacac gagccgtcgt ttcgcggcgc gccgcaaatc
      361 agaagaacac gcgacgccgt atcgggatcg gtttgtttgg cggctgcggc ccgcacgcgc
      421 tcgcgcgtct ccagccactc gaccgagatg tcgtaatccg gatcggcgaa ctccgggcct
      481 gccttgcgcg tgaccggcgc cttgcgatct tcgtggtacc cgttccacgc aatcgcctcg
      541 acacggtcga gcgcggcttg ctcgccccgg aacgcagggt cgaagtaggc cgagcggaag
      601 cgcttgccga actcgacgcg cgaatgcggc ggcggcgcct gtcccttgcg gaccttgatc
      661 atggtttttt gattcgcgcg ccggccgagc cgacttttcc cccagacgcg aaggctacgg
      721 tagcacgaca ggcgcgtctg tcggtgcgcg gccgtatttt ttgtccgcgc aacgcagcgt
      781 cattgtagga cgccggctga cattgccctg ccggcgggct gacgcgtccc ggccgtcgcc
      841 ttcagaaggc gctcggccgg agcgcagtct gcgcttacca ggcgagctcg acggtggcgt
      901 cgatgtcggc gccggtgtgc gcggcggata cgaagcctgc ttcgaacgcc gagggcgcca
      961 agtagacgcc ggcatcgagc atgcgatgaa aaaaacgatt gaacgcttcc ttgtcgcacg
     1021 ccatcaccgc cgcgaacgaa tcgggcacgg tcgccgaaaa ataaatgccg aacatcccgc
     1081 cgatcgactg cgcggaaaac gcgacgcccg ctcttgccgc cgccgcggtg aggccatcgg
     1141 tcagcgcccg ggtacgctct gccagccgct ggtgaaatcc cggcgactgg atcgccttca
     1201 gcgtcgcgag tccggcggcg acggctaccg ggtttcccga caatgtgccg gcctgataaa
     1261 cggggccgag cggcgcaatc ttttccatga tcgcgcgctt tccgccgaat gcgccgaccg
     1321 gcatgccgcc gccgatcacc ttgcccagcg tcgtcaggtc gggcgctacg ccatagaggc
     1381 cctgcgcgcc ttgcaggtgc aggcgaaaac cggtcatcac ttcgtcgaag atcagcaccg
     1441 cgccgtgccg atcgcagatc tcgcgcaggc ccgccaggaa tcccggcttc ggcgcgatca
     1501 ggttcatgtt gcctgcgacc ggctcgacga taatgccggc gatcgcatcg gggtgctcgg
     1561 caaaggcgcg cgccacgtcg tccagatcgt tgtacgtcag cacgagcgtc tcgccggcga
     1621 tcgatgccgg gacgccggcc gacgacggtt gtccgaacgt cagcgcgccc gaaccggcct
     1681 tgaccagcaa gctgtcacca tggccgtggt agcagccttc aaatttgatt attttcgagc
     1741 ggccggtgta gccgcgcgcg aggcgcagcg ccgtcatcgt ggcttcggtg cccgacgaca
     1801 ccagccgcac catctcgagc gaaggcagca gacggcacag ctcttccgcc atctcgatct
     1861 cgagctcggt cggcgcgccg aacgacaagc cgtgtcgggc ggtctcctgc accgcgcgca
     1921 ccgtctccgg atgcgcgtga cccaggatcg ccgggcccca cgagccgaca tagtcgatat
     1981 agcgcttgcc atcggcatcc cacaggtagg cgccttcgcc gcgggtaaag aatcgcggcg
     2041 tgccgcctac cgagcgaaac gcgcgcaccg gtgaattgac tcctgcggga atcgtctttt
     2101 gcgcgcgtgc gaacagctgt tcgtttcggg tcatggtctc gaaccggtga gcgtagaggg
     2161 atgggtgaaa gacggggact cgtgtcgatg gggatgaggc gcgagcttgc gcagtcgcgg
     2221 gcgcacgtgt ggcatcagcc gaagcacgcg accagctcgc gcgccgccaa caggacgtca
     2281 ggcgcatcgt agagggcgct gatcaccgcc accgcatcgg cacccgcagc gataagcggt
     2341 gtcgcattgg ccgcgttgat tccgccgatg gcgacgacgc cgacccccgg ccagcgcgcc
     2401 tttgcctcgg cgatcagcct gagcgacggc cgaacggcgt tcggcttcac cagcgaggga
     2461 taaaagctgc cgaacgcgat gtaatcgccg ccgtcggcga tcgccgcctc tgcgcgcgcg
     2521 agctcgtcgt agcatgaaac gccgacgatc gccgcaggcc cgagccccgc gcgcgccgcc
     2581 gcgacgctcg cgtcgtcgcg gcccagatgc acaccgtcgg cgccgaccgc gcgcgcgagc
     2641 tcgacatcgt cgttgacgat gaagattgcc ccgcgcgccg agcacagcgc acgcaagctc
     2701 gccgcctgct gcaacctgag ttcggccgag gcggccttgt tgcggtactg gatcgccgat
     2761 gcgccgccgg atagcgcgcg gtcggttcgc gccaatagcg cctccgtgtc ggcgagatcg
     2821 ggggtcagcg cgtagaggcc ggcaagcttc ggccgcggca cggtcctcga tcccgcggcg
     2881 gccatcagtg cggcttcagt gccgatgcag atcgggcgac gccggcagaa cgtcttcgtc
     2941 gacgcgaggc tcggcgtcgt cctcccgcgc ccagaacaag cggtctggaa gaaactgacc
     3001 cat
//
LOCUS       DATANS010000798         2807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_755013, whole genome shotgun sequence.
ACCESSION   DATANS010000798 DATANS010000000
VERSION     DATANS010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755013"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VHT22_11855"
     CDS             <1..978
                     /locus_tag="VHT22_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX3138300.1"
                     /translation="APAPPAQATVAPSAPIDDAAFRKAHASPSVRAHARALGVDLSQI
                     KGTGPVGRILQQDLENYVKQVMSGAKVASAVAGAGGAALGLLPWPQIDFTKFGPVETR
                     PLSRIKKIAGANLHRNWVLIPHVTNHDDADITELEAFRVQLNKENEKAGIKVTLLAFL
                     IKACVWALKKFPEFNASLDGENLVLKQYYHIGFAADTPNGLVVPVIRNADQKGVLAIA
                     AEMGELSAKAREGKLGPADMQGGCFSISSLGGIGGTYFTPIINAPEVAILGVCRSSTR
                     PVWDGKQFAPRLILPLSLSWDHRVIDGAAAARFNSYLASLLADMRRTML"
     gene            980..1789
                     /locus_tag="VHT22_11860"
     CDS             980..1789
                     /locus_tag="VHT22_11860"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3138301.1"
                     /translation="MGVDKHWLTLVLLAALACPCFAAEELLSIPTRNGVTLSYLLDQD
                     KAAAPKVVVISFIGGLGAIGLERRAQNGPVKFGPTANFLIRIRDRMTDADIADVIVDS
                     PSDQLPGGMSDRFRSGQDHATDIRALISDVKRRFPGAKIFLLGTSRGTISAASLGASL
                     GDVVQGVVLSSTVTNADRTGSALSTFDFGSIKVPVLLVHHRDDGCRSSPYYGAERLAK
                     RFPLVSVSGGDPPQSEPCEAMSQHGYLGREAPTTEAIRNWMLGREFAREIQ"
     gene            1845..>2807
                     /locus_tag="VHT22_11865"
     CDS             1845..>2807
                     /locus_tag="VHT22_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3138302.1"
                     /translation="MSTIEVKVPDIGDFTDVPVIEVLVKPGDTVKVEDSLVTLESDKA
                     TMDVPAPAAGVVKSLGVKVGDKVSEGALLLTLETTAEAPAARPAGTGSAQQAPTERAA
                     PAAQGPPAAAKPQPAPPAAPAATAPRAADEYRGAVDIECQMVVIGSGPGGYSGAFRAA
                     DLGLKTVLIERYSTLGGVCLNVGCIPSKALLHAARVIDEAEEMGHFGVTFGKPAIDLA
                     KLRGWKEAVVGKLTRGISGLAKQRKVSVVTGVARFVDTHHLEVSAADGKKQVVKFEHC
                     IIAAGSQAVKLPFIPDDPRVIDSTGALELESMPGHMLVIGGGIIG"
BASE COUNT          495 a          899 c          949 g          464 t
ORIGIN      
        1 gcgcccgccc cgcccgcgca agcaaccgtg gcgccgagcg ctccgatcga cgatgcggcc
       61 ttccgcaagg cgcacgcctc gccgtcggtt cgcgcccacg cgcgcgcact gggtgtcgac
      121 ctgtcgcaga tcaaggggac cggcccggtc gggcgcatcc ttcagcagga cctcgagaac
      181 tacgtcaagc aggtgatgag cggcgccaag gtcgccagcg cggtcgccgg cgcgggaggc
      241 gccgcgctcg gcctgctgcc atggccgcag atcgatttca ccaagttcgg cccggtcgaa
      301 accaggccgc tgtcgagaat caagaagatc gccggcgcca atctgcatcg caactgggtt
      361 ttgattccgc acgtcaccaa ccatgacgac gccgacataa ccgagctcga ggcgtttcgc
      421 gtgcagctca acaaggaaaa cgaaaaagcc gggatcaaag tgaccttgct ggcgtttctg
      481 atcaaggcgt gcgtctgggc gctgaagaaa tttcccgagt tcaacgcttc gctcgacggc
      541 gagaacctgg tgctcaagca gtactaccac atcggtttcg ccgccgacac gccgaacggt
      601 cttgtcgtgc cggtcatcag gaatgccgac cagaagggcg tgctggcgat cgccgcagag
      661 atgggggagc tctcggcgaa ggcgcgcgaa ggcaagctcg gtccggccga catgcagggc
      721 ggatgctttt cgatctccag cctgggcggg atcggcggaa cgtatttcac gcctatcatc
      781 aacgcacccg aagtcgcgat cctcggcgtg tgccggtcga gcacgcgccc cgtctgggac
      841 ggcaagcagt tcgccccgag gctgatactg ccgctgtcgc tttcctggga ccatcgcgtg
      901 atcgacgggg cggcggccgc ccgcttcaac agctatctcg cctcgctgct ggccgacatg
      961 cggcgcacga tgctttgaga tgggcgtcga caaacactgg ctcaccctcg tgctgctggc
     1021 cgcgctggcg tgtccctgtt tcgcggcgga ggaattgctc agcattccga cccgcaacgg
     1081 cgtcacgctg tcctacctgc tcgaccagga caaggccgcg gcgcccaagg tcgtcgtcat
     1141 ttccttcatc ggcggattgg gtgcgatcgg cctggaacgg cgtgcgcaaa atggcccggt
     1201 gaaattcggg ccgacggcga atttcctgat tcgcatcagg gaccggatga ccgacgccga
     1261 catcgccgac gtcatcgtcg attcgccttc ggatcagctt cccgggggca tgagcgaccg
     1321 gttccgctca gggcaggatc acgcgacgga cattcgggca ttgataagcg atgtgaagcg
     1381 gcggtttcca ggcgcgaaga tttttctgct cggcacgagt cgcggcacga tttcggcggc
     1441 ctcgctgggc gcgagtctcg gcgacgtcgt gcaaggcgtc gtcctcagct cgaccgtgac
     1501 caatgccgac cgcacaggca gcgccctgtc gaccttcgat ttcggctcga tcaaggttcc
     1561 ggtgcttttg gtccatcacc gggacgacgg ttgccgcagc agcccgtact acggcgcgga
     1621 gcgcctggcg aagagatttc cgctggtcag cgtcagcggc ggcgatccgc cgcagagcga
     1681 gccttgcgag gcgatgtctc agcacgggta cctgggccgc gaagcgccga cgacggaggc
     1741 gatccggaac tggatgctcg gccgcgagtt cgcgcgcgag atccaatgat gatgttgtca
     1801 ctgccggtat cgacacactg ctgccggatc cgatcgggga cgcaatgagc acgatcgaag
     1861 tcaaggttcc tgacatcggc gatttcaccg atgtgccggt gatcgaagtc ctggtcaagc
     1921 cgggcgacac ggtgaaggtc gaggattcgc tggttacgct cgagtcggac aaggcgacga
     1981 tggacgtgcc ggcgccggcg gccggagtag tcaagtcgct cggcgtcaag gtcggcgaca
     2041 aagtcagcga aggcgcactg ctgctgacgc tcgagacgac ggccgaagcg cccgcggcgc
     2101 ggcccgccgg caccggatcg gcgcagcagg cacccacgga gcgagccgcg ccggcggcgc
     2161 agggcccgcc tgcggcggcc aaaccgcagc cggcgccccc ggccgcgcct gcagcgaccg
     2221 cacctcgcgc cgccgacgaa tatcgcggcg cggtcgacat cgaatgccag atggtcgtta
     2281 tcggctccgg gccgggcggc tacagcggcg cgttccgtgc tgcggactta gggctcaaga
     2341 ccgtgctgat cgagcgatat tcgaccctgg gcggcgtgtg cctcaatgtc ggctgcattc
     2401 cttcgaaggc gctgctgcat gcggccaggg tgatcgacga agcagaggag atgggtcact
     2461 tcggcgtgac tttcggcaag ccggcgatcg atctggccaa gctgcgcggc tggaaggaag
     2521 cggtggtcgg aaagctgacc cgcggcatct ccgggctggc gaagcagcgc aaggtgagcg
     2581 tcgttaccgg agtggcgcgc tttgtcgata cgcatcacct agaggtcagc gccgcggacg
     2641 ggaagaagca agtcgtcaag ttcgagcact gcatcattgc cgccggctcg caggcggtga
     2701 agctgccatt cattcccgac gacccgcgcg tcatcgattc gaccggtgcg ctcgagctcg
     2761 agagcatgcc cgggcacatg ctggtcatcg gcggcggcat catcggc
//
LOCUS       DATANS010000799         2511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_376531, whole genome shotgun sequence.
ACCESSION   DATANS010000799 DATANS010000000
VERSION     DATANS010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376531"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VHT22_11870"
     CDS             complement(<1..558)
                     /locus_tag="VHT22_11870"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3138303.1"
                     /translation="MVLTSRYQDIFRGSGFRMNPVLTLALRSLVRNRRRSAIALAAVG
                     FGVAALILADGFVDSLLVKLREDTIYSQLGHIQIRDPKFGELGLSEPYGHLLPTADGD
                     MAPVARLPHVRVVAPRLALSGLVSLGDTTLSFTADAIDPAKEAMFDRGLAITEGAALG
                     ADDKEAVILGEGLAANLGAKTGDKVV"
     gene            complement(574..664)
                     /locus_tag="VHT22_11875"
     tRNA            complement(574..664)
                     /locus_tag="VHT22_11875"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(628..630),aa:Leu,seq:taa)
     gene            complement(691..1491)
                     /locus_tag="VHT22_11880"
     CDS             complement(691..1491)
                     /locus_tag="VHT22_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3138304.1"
                     /translation="MTGRQGAASWGAVALVRTVIIAGVALAWQMLAVSGLLFRDVVPP
                     LGVIGSAVVRLLADEGFYRNVVITLGEIAAALAIGGVAGLGVGILLGANRFLSRAYES
                     LIYYLGPTPKIIFFPVLIMWFGVGPGSKIAMGVLSCFFPIAISAAAGMRQIDPILIRV
                     GQSFRASIRQMIVKVYLPAMREPIVNGVRLGLGVAIIGTLLAETKLSNRGIGYLIIQA
                     YSTFDMPRMYALLIVLFTLAVGANAMLGGLARSSSAEPAPVATRMPVQ"
     gene            complement(1488..2273)
                     /locus_tag="VHT22_11885"
     CDS             complement(1488..2273)
                     /locus_tag="VHT22_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3138305.1"
                     /translation="MRQLSPMGAGALSPRLGPRTVGLATTIAVIVLIEILIRVGVINR
                     FIVPLPSQILGALPRIIAEENVLHRFGQTTQELFWASLLLAVTGIALGALLYRFQLLR
                     AACETWVAALAAAPIVLMYPLFLVIFGRSATTIVMIGFAAGLAPVILKTLEGLTRTRQ
                     VLIDVGRSFRLTPAQEFRKILLPAALPSIFVGLRLGLIFAMINIVGVEFLINFGGLGQ
                     LINELAERYDLPATYGAICFVILASIVFFDITGRIERWLRPNE"
     gene            complement(2270..>2511)
                     /locus_tag="VHT22_11890"
     CDS             complement(2270..>2511)
                     /locus_tag="VHT22_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate ABC transporter ATP-binding protein"
                     /protein_id="HEX3138306.1"
                     /translation="THNLTEAVQLSDRVLVMTYRPGRLKRIVTIDLPRPRDSEVVGSA
                     AFGRYVAEIWHDLREEASRGIQEDESRVLQGGRGK"
BASE COUNT          503 a          819 c          801 g          388 t
ORIGIN      
        1 tacgacctta tcgcccgtct tcgcccccag attcgcggcg aggccctcac ccaggatgac
       61 cgcctccttg tcatcggcgc cgagtgcggc gccctcggtg atcgccaggc cgcgatcgaa
      121 catcgcttcc ttggcagggt cgatcgcgtc cgcggtaaaa gacagcgtcg tgtcgccgag
      181 actgacaagc ccgctcaagg ccagtcgcgg agcgacgacg cgcacatggg gaaggcgcgc
      241 gacgggcgcc atatccccgt cagccgtcgg gagaaggtgg ccgtagggct cgctcaagcc
      301 caattcgcca aacttcggat cccgaatctg gatatggccg agctgtgaat agatcgtgtc
      361 ctcgcgcagt ttgaccagca gcgagtcgac gaagccgtcg gcgagaatga gcgcggcaac
      421 gccgaaaccc accgcggcca acgcaatcgc gctgcgccgg cgattgcgaa ccaggctgcg
      481 cagggcgagc gtgaggacgg gattcatccg gaagccggat ccacggaaga tgtcctggta
      541 gcgggacgtc agaaccaccg gcaatcgatc gaatggtgcc cagggccgga ctcgaaccgg
      601 cacgacgcgt ttcggcgtcg gcggatttta agtccgctgt gtctaccagt ttcaccaccc
      661 gggcgagtgc tcatcctgcc gtggcggcat tcattgtacc ggcatcctgg tcgcgacagg
      721 cgcaggctcg gcggagctcg accgggcgag ccccccgagc atcgcattcg cgccgacggc
      781 aagcgtgaac aggacgatca gcagcgcgta cattcgcggc atgtcgaatg tcgagtaggc
      841 ctggatgatc aggtagccga tgccgcgatt cgacaatttc gtctccgcga gcagtgtccc
      901 gatgatcgcg acacccaggc ccagccgcac gccgttgacg atcggctcgc gcatcgccgg
      961 cagatagacc ttgactatca tctgccggat cgaggcccgg aagctttgtc cgacgcggat
     1021 cagtatcgga tcgatctgcc gcattccggc ggctgcgctg atcgcgatcg ggaagaagca
     1081 cgaaagcacg cccatcgcga tcttggatcc gggcccgacg ccgaaccaca tgatcaggac
     1141 cgggaagaaa atgatcttcg gcgtcggtcc gaggtagtag atcagcgact cgtaagcgcg
     1201 cgacaggaag cgatttgccc cgagcagaat gccgacgccc aagccggcga caccgccgat
     1261 cgcgagcgcc gccgcaatct ccccgagagt gatcacaacg ttgcgataga agccttcgtc
     1321 ggccagcagg cgaactaccg cgcttccaat cacgcccagc ggtgggacga cgtcgcggaa
     1381 cagcagcccg gaaacggcga gcatttgcca ggccagcgcg acgcccgcga tgatgacggt
     1441 ccgcaccagc gcgacggcgc cccaggacgc agcaccctgc ctgccggtca ttcgttcggc
     1501 cgcagccaac gctcgatcct tccggtgata tcgaaaaaca cgatgctcgc cagaatgaca
     1561 aagcagatcg cgccataggt tgcgggcaag tcgtagcgct cggccagctc gttgatcagc
     1621 tgtccgagcc cgccgaaatt aatcaggaat tcgacgccga caatgttgat catcgcgaag
     1681 atcagcccga ggcgcagacc gacgaaaatc gacggcagcg ccgccggcag aagaatcttg
     1741 cggaactcct gcgccggcgt caggcggaag ctcctcccga cgtcgatcag cacctgccgg
     1801 gtcctggtga ggccttccag cgtcttgagt atcactggcg cgagcccggc ggcaaagccg
     1861 atcatgacga tcgtcgtcgc actgcgtccg aatatcacca ggaacaacgg atacatcagg
     1921 acgatgggcg ccgcggcgag cgcggcgacc caggtctcgc aggcggcgcg cagcagttgg
     1981 aagcggtaga gcagggcgcc gagcgcgatg ccggtcaccg cgagcagcag gctcgcccag
     2041 aagagctcct gcgtcgtctg cccgaagcgg tggaggacgt tttcctcggc gatgatgcgc
     2101 ggcagcgcgc cgagaatctg cgacggcagc ggcacgataa aacgattgat cacgccgact
     2161 cggatcagga tttcgatcag cacgatcacg gcgatcgtcg tcgccaggcc gaccgtccgc
     2221 ggaccgagcc gcggcgatag agcgccggcg cccattgggc tcagttgcct catttgcccc
     2281 tgccgccctg gaggactcgc gattcgtcct cttgaatacc gcggctggcc tcttcgcgca
     2341 ggtcatgcca gatctcggcg acatagcggc cgaacgctgc gctgccgacg acttcggaat
     2401 cgcgcggacg cggaaggtcg atggtgacga tgcgcttcag gcgtcccggc cgatacgtca
     2461 tcaccagcac acggtcggat agctgcaccg cctcggtcag attgtgcgta a
//
LOCUS       DATANS010000800         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_123320, whole genome shotgun sequence.
ACCESSION   DATANS010000800 DATANS010000000
VERSION     DATANS010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123320"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /gene="thpR"
                     /locus_tag="VHT22_11895"
     CDS             <1..619
                     /gene="thpR"
                     /locus_tag="VHT22_11895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX3138307.1"
                     /translation="TCHKPPRERQMAAAPRWARAVLFYIGAMADPKLRLFFALWPDPQ
                     TRASLAALAERVAVETGGRATTPNNVHLTLAFLGAQPRDSATELIARAGAIDASPFVL
                     TLDHIDCWRKNAIAWLGASEIPPALASLREALLASLAPLHIADEARPFAVHVTLARKV
                     TGLLRRRLPAPIAWPVDGLALVASDTGSAGPVYRVLSSLPLVRRR"
     gene            complement(645..1496)
                     /locus_tag="VHT22_11900"
     CDS             complement(645..1496)
                     /locus_tag="VHT22_11900"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX3138308.1"
                     /translation="MYTSLISTDELARQLSDPALVLVDCRHNLSDVDAGQRAYGASHL
                     PGARFMHMDRDLSGERTGGNGRHPLPDVAALSGSLSRAGIDASKQVVAYDQNNGMWAS
                     RLWWLLHWLGHDAAAVLDGGIDKWIAEGRPITADRPSVQSARFVAMTPRPVISSADIL
                     RGLSNHSSNPLTIIDARAPERFRGDIEPLDPVAGHIPGAINRPYGANLTPQQTFKPAE
                     LLRAEFDAQLGGAPLSSVVHQCGSGVTACHNLLAMEVAGLPGSRLYPGSWSEWVADPA
                     RPVARGT"
     gene            complement(1507..>2020)
                     /locus_tag="VHT22_11905"
     CDS             complement(1507..>2020)
                     /locus_tag="VHT22_11905"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX3138309.1"
                     /translation="IGGGFTSRLNLDLREEKGYTYGIGSRFEYGRRPGPFVVRTAVKS
                     DVAVPAIREILAQLGRAGAAPLTPEELQRARGSLTRSLPAMFETNGATGSSFGELFAY
                     ALPLDYFRQLPSQLTKVGAADLPPLARRYFDPASMVVIAVGDRASLQPALEALKLGPL
                     EVWPIGDKLF"
BASE COUNT          325 a          690 c          669 g          336 t
ORIGIN      
        1 gacttgtcac aagccgccgc gcgaacggca gatggcggcc gccccgcgat gggcccgcgc
       61 cgttttgttt tatattggcg cgatggccga tccgaagctg cgactgttct tcgcgctatg
      121 gcccgatccg caaacgcgag cctcgctcgc cgcgctggcc gagcgcgtgg cagtcgagac
      181 cggcggccgc gcgacgacgc ccaacaatgt ccatctgacg ctggcgtttc tcggcgccca
      241 gccgcgcgat agcgcgaccg agctcatcgc gcgggcaggc gcgatcgacg cgtcgccctt
      301 cgtactcact ctcgaccaca tcgattgctg gcgcaagaac gctattgcct ggctgggcgc
      361 gagcgagatt ccgccggcgc tcgcttcgtt gcgcgaggcc ttgctcgcct cgctcgcccc
      421 gctgcacatc gccgacgaag cgcgtccgtt tgccgtgcac gtcacgctgg cgcgcaaggt
      481 caccggcttg ctgcgccgcc gtttgcccgc gccgatcgcc tggccggtcg atggattggc
      541 gctggtcgcg tcggacacgg gaagcgctgg tccggtctat cgcgtgctct ccagcctgcc
      601 gctcgttcgg cggcgttgat cgtggattag ggcctgaaca ggccctaggt gccacgcgcg
      661 actggacgcg ccggatcggc gacccactcg ctccacgagc caggatagag ccgcgagccc
      721 ggcagcccgg cgacctccat ggccagcagg ttgtggcacg ccgtcacgcc cgagccgcat
      781 tgatgcacga ccgacgacaa aggcgcgccc ccgagttgcg catcgaattc ggcgcgcagc
      841 aactcggccg gcttgaatgt ttgctgcggc gtcaggttgg cgccgtaagg ccgattgatc
      901 gcgccaggaa tatgcccggc aaccggatcg agcggctcga tatcgccgcg aaagcgctcc
      961 ggcgcgcgcg cgtcgattat cgtcagcggg ttcgacgagt gattgctcaa gccccgcaga
     1021 atatcggcgc ttgagatgac cggcctcggc gtcatcgcaa cgaaccgggc cgattgaacg
     1081 ctcggccgat cggctgtgat cggccgccct tcggcgatcc atttgtcgat cccgccgtcc
     1141 agcaccgcag ccgcatcgtg gccgagccag tgcagcagcc accacaggcg agatgcccac
     1201 atgccgttgt tctgatcgta ggcgacgacc tgcttggacg catcgatgcc ggctcgactc
     1261 aagcttccgg acaatgcggc gacgtccggc agcggatggc ggccgttgcc tccggttcgc
     1321 tcgccggaca ggtcgcgatc catgtgcata aagcgcgcgc cgggcagatg cgaagcgccg
     1381 taagcgcgct gacccgcgtc gacgtcgctc aagttgtgac ggcaatcgac gagcaccagc
     1441 gcagggtcgc tcagctgccg cgcaagctcg tcggtcgaga tcaggctcgt atacatcgcc
     1501 gctttgtcag aacagtttgt cgccgattgg ccagacttcg agcggcccca gcttcagcgc
     1561 ctcgagcgcg ggctggaggc tggcacgatc gccaaccgcg atcacgacca tcgacgccgg
     1621 atcgaaataa cgccgcgcca gcggcggcag atcggcggca ccgactttgg tcaattgcga
     1681 cggcagctgg cggaaatagt cgagcggaag tgcgtaggcg aacaattcgc cgaacgagct
     1741 tccggtggca ccgttcgttt cgaacatcgc cggcagtgac cgggtcagcg agccgcgagc
     1801 gcgctgcagc tcctccggtg tcagcggcgc ggcgcccgcg cgcccgagct gggcaagaat
     1861 ttccctgatc gccggcaccg cgacgtcgct tttcaccgcg gtgcgcacga cgaacggccc
     1921 cgggcggcgt ccgtattcga agcgggagcc gatgccgtag gtgtagcctt tctcttcgcg
     1981 caagtcgaga ttgagtcggc tggtgaagcc gccgccgatg
//
LOCUS       DATANS010000801         1588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_835624, whole genome shotgun sequence.
ACCESSION   DATANS010000801 DATANS010000000
VERSION     DATANS010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835624"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..518)
                     /locus_tag="VHT22_11910"
     CDS             complement(96..518)
                     /locus_tag="VHT22_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX3138310.1"
                     /translation="MLRQSSTMLGFDTRGRGLIEITRPVVDWVAGSEMESGLLTLFVR
                     HTSASLVVQENADPDVRGDLERFLARLVPDGDSFFRHRDEGADDMPAHVRAALTAVQL
                     SIPLSGGRLALGTWQGIYRWEHRIRPHRREVALHLMGS"
     gene            635..>1588
                     /locus_tag="VHT22_11915"
     CDS             635..>1588
                     /locus_tag="VHT22_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3138311.1"
                     /translation="MQAGFNFEPLTPTAFLHRAAAIFPDRIGVIDGSLELTYREFLDY
                     ALKFAGVLKALGVEPGHRVAVLAGNSHVMLAAHYAVPFAGAVLVALNTRITSADIAYI
                     LDHAGASVLVYDQKFAAVAEAAGEQAGRRLRLVRAGGEPDELEGLIANASPAFESVGD
                     ERSLLAINYTSGTTGRPKGVMYHHRGAYLQALAMALHMGLDSDSVYLWTLPMFHCNGW
                     CFPWGVTAMGGAHLCMDKVEPVAIWRQLRESGVTHLCAAPTVLTTTIWDAAAGREKLP
                     RRIRIATGGAPPTPALLERLAELGMDITHLYGLTETFGPAVV"
BASE COUNT          278 a          516 c          509 g          285 t
ORIGIN      
        1 ggaaacgggc acggatccgc aacgccaagg atgctcgggt agcgggcaag ctcgtcgagc
       61 cgacgtatcg gctgtcccat ttggtcgtga tgacgtcagc tgcccatcag atgcaaggcg
      121 acttcgcgcc gatgcggacg aatcctgtgc tcccagcggt agatcccttg ccatgtgccc
      181 agcgcgaggc gtccgccaga gagcggaatc gagagctgga ccgcggtcaa cgcggcacgg
      241 acatgcgcag gcatgtcatc ggctccctcg tcgcggtgcc ggaagaagga atcgccatcg
      301 ggcaccaggc gagccaggaa gcgttcgaga tccccgcgca cgtcaggatc ggcattctcc
      361 tgcacgacta gactcgctga cgtgtggcgg acgaacagcg tcagcagtcc gctttccatc
      421 tcgctgccgg ccacccaatc gacgaccgga cgagtgatct cgataagacc tcgtccgcgc
      481 gtgtcgaagc cgagcatcgt gctggattgg cgaagcatcc ggcgaagcat agatcaaggc
      541 ggcccgcgcg ggcgaatccg tatttcaaac cggggtgttt tttgcggccg gcgcgaacga
      601 cttccggtat tctgaagagc ccaggagcga gccgatgcaa gccggtttca atttcgaacc
      661 cctgacaccg accgcgtttc ttcatcgcgc agcggcgatc tttcctgatc gcatcggggt
      721 catcgacggc agcctcgagc tcacctatcg cgagttcctc gactacgccc tcaaatttgc
      781 cggtgttctg aaagcgctcg gcgtcgagcc gggccaccgt gtagcagtgc tggcgggcaa
      841 ttcgcacgtg atgctggccg cgcactacgc ggtgccgttc gccggcgcgg ttctcgtcgc
      901 gctcaatacg cgaatcacgt ccgccgacat cgcctatatc ctggatcacg ccggcgcgtc
      961 ggtccttgtc tacgaccaaa aattcgcggc ggttgccgaa gctgccggcg aacaggccgg
     1021 gcggcgatta cggctcgttc gggcgggcgg tgagcccgat gagctggaag ggctcatcgc
     1081 caacgcttcg cccgctttcg agtcggtcgg cgacgagcgt tcgctgctcg ccatcaatta
     1141 caccagcggc acgaccggcc gtcccaaagg cgtgatgtat caccatcgcg gcgcgtattt
     1201 gcaggccctc gcgatggcgc tccacatggg actcgacagc gactcggttt atctgtggac
     1261 gctgccgatg tttcactgca atggctggtg tttcccctgg ggcgtgacgg cgatgggagg
     1321 ggctcacctg tgcatggaca aggtggaacc cgtcgcgatc tggcgccagc tgcgagagtc
     1381 aggcgtcacc cacttgtgcg ctgcgccgac ggtgctgacc acgacgatct gggacgctgc
     1441 cgcgggcagg gagaaattgc cgcggcgcat tcggatcgcg accggcggcg ccccgccgac
     1501 acccgcgctg ctcgagcgcc ttgcggagct cggcatggac atcacccacc tctacggcct
     1561 gaccgaaacc ttcggtcctg ccgtcgtc
//
LOCUS       DATANS010000802         2422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_544312, whole genome shotgun sequence.
ACCESSION   DATANS010000802 DATANS010000000
VERSION     DATANS010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544312"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..562
                     /gene="rimI"
                     /locus_tag="VHT22_11920"
     CDS             62..562
                     /gene="rimI"
                     /locus_tag="VHT22_11920"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005220589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX3138312.1"
                     /translation="MSAGVEAGIEATVIWRPMREEDLPVVAALEQDSHAAPWSEGNFR
                     DAFAAGYGTLVGELDRSVVAYGVLMLAPGEAQLLNLTVAAPARRRGLGRRLLRRLLDD
                     AVGVGAEQCFLEVRESNHAAIALYLREGFAPVARRAGYYPVPGSDGGREDALVMRRRL
                     GRDSRG"
     gene            555..1268
                     /locus_tag="VHT22_11925"
     CDS             555..1268
                     /locus_tag="VHT22_11925"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX3138313.1"
                     /translation="MVDRSDVLRELGLLPVWRLRPLPDVGPGVGAAERVARIATLGWS
                     DFAADVQACTACGLCRSRRQAVPGVGDAGAEWMFVGEAPGAEEDAKGEPFVGQAGRLL
                     DNMLAALGMHRGTNVYIANVLKCRPPGNRTPEPGEVDACRPYLDRQIELIRPKLIVAL
                     GKSAATTLLAVDATIASLRGRVHRYQGVPLIVTYHPAYLLRNLPDKAKAWEDLCLARA
                     TIRALHDAGGLQSVAAAPK"
     gene            1347..1997
                     /locus_tag="VHT22_11930"
     CDS             1347..1997
                     /locus_tag="VHT22_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX3138314.1"
                     /translation="MRKNSGLAPAVFAAVFATLLLSGCGYNDLQRQDEQVKSAWSEVV
                     NQYQRRADLVPNLVNTVKGYAAQEQQVLTEVTNARASVGSIKATPELLNDPAAFAKFQ
                     AAQGELQSALSRLLVVAENYPNLKSDALFRDLSSQLEGTENRITVARNRFIKAVQEFN
                     TTVRSFPTNLTAMLFKMEVKPNFTVDNEKAISAPPKVDFSKPPAQPAPAPAPAPKS"
     gene            2102..>2422
                     /locus_tag="VHT22_11935"
     CDS             2102..>2422
                     /locus_tag="VHT22_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX3138315.1"
                     /translation="MLASVAFAVAGAPNDGLAPIPPLASAVTDQTGTLSASERPALEA
                     KLRDWEARTTNQLAVLIVPTTAPEPIEQYSIRVAEAWKIGRKGVDDGLLLIVAKNDRR
                     LRIEV"
BASE COUNT          404 a          794 c          842 g          382 t
ORIGIN      
        1 cgatttacct gcggcacaag gtcgcgctga ccagcgccga gcgcgccgtt ggacgagcgc
       61 gatgagcgcc ggtgtcgaag cgggaatcga agcgacggtg atctggaggc cgatgcggga
      121 ggaggacttg ccggtggtcg cggcgctcga gcaagactcg cacgcggcgc catggagtga
      181 aggcaatttc cgcgacgcct ttgccgccgg ttacgggacg ctggtcggcg agctcgaccg
      241 cagtgtcgtc gcgtacggcg tgctgatgct cgcgccgggc gaggcgcaac tcttgaatct
      301 gacggttgcg gcgccggcac gccggcgggg cttggggcgc aggctgttgc gacggctgct
      361 ggacgatgcc gtgggcgtcg gcgccgagca gtgttttctg gaggtccgcg aatcgaatca
      421 tgcggccatc gcgctttatc tgcgcgaagg attcgcgccg gtcgctcgcc gcgccggcta
      481 ctatccggtg ccaggcagcg acggcgggcg cgaagatgcg ctggtgatgc gtcgccgcct
      541 cggaagggat tcgcgtggtt gatcgcagcg acgtcctgcg cgagctgggc ctgttaccgg
      601 tatggcggct gcgcccgctg cccgatgtgg gaccgggggt cggagcggcg gaacgcgtcg
      661 cgcgcatcgc gacgctcggc tggtcggact tcgccgccga tgtgcaggcg tgtaccgcat
      721 gcggattgtg ccgctcgcgc cggcaggcgg tgccgggagt cggcgacgcc ggcgccgaat
      781 ggatgtttgt cggcgaggcg ccgggcgccg aagaggacgc caaaggggag ccattcgtcg
      841 gacaggccgg acggcttctc gacaatatgc tcgcggcgct gggcatgcac cgcgggacca
      901 acgtctacat cgccaacgtg ctcaaatgcc gtccacctgg caaccgcacg cccgaacccg
      961 gcgaagtcga tgcctgccgg ccgtatctgg accgccagat tgaactgata cgcccgaagc
     1021 tcatcgttgc gctcggcaaa agcgcagcga cgaccctgct cgccgtcgat gcgacgatcg
     1081 cgagcctgcg cggccgcgtg caccgctatc agggcgtgcc gctgatcgtc acctatcatc
     1141 cggcgtatct tttgcgtaac ttgcccgaca aagccaaggc atgggaagat ctctgcctcg
     1201 cgcgggcgac gattcgcgct ttgcatgacg ccggcggctt gcaaagcgtg gcggcggccc
     1261 ccaaataaag gccggcgggc aagcttgccc gaggaacgca cgccgcgcgg ccatcgaacg
     1321 cgcggcgact tctgggaggg aatggcatgc gcaagaattc tggcttggcc ccggcggtct
     1381 tcgccgcggt gttcgcgacg cttctcctgt ccggctgcgg ctacaacgat ttgcagcgtc
     1441 aggacgagca ggtcaagtcg gcatggtcgg aggtggtcaa ccagtaccag cggcgcgccg
     1501 acctggtgcc gaacctggtc aacaccgtca agggctacgc cgcccaggag cagcaggtcc
     1561 tgaccgaggt gaccaacgca cgagccagcg tcggcagcat caaggcgacg ccggaactgc
     1621 tcaacgatcc ggccgcattc gccaagttcc aggccgcgca gggcgagctg caatccgcgc
     1681 tctcccggtt gctcgtcgtc gcggaaaatt acccgaacct gaagtcggac gcgctgttcc
     1741 gggacttgag ctcgcagctc gaaggcactg agaatcgaat caccgtcgcg cgcaacaggt
     1801 tcatcaaggc ggtgcaggag ttcaacacca cggttcgctc gtttccgacc aacctgaccg
     1861 cgatgctgtt caagatggaa gtgaagccga acttcaccgt cgacaacgaa aaggcgatca
     1921 gcgcgccgcc gaaggtcgat ttcagcaagc cgcctgcgca acctgcgcct gcgccggcac
     1981 cggcgccgaa gagctgagca ccgcacgccg cgcggtcagc cgcgcggcga tgcggctctc
     2041 gcgccgcttg atgatccatt cccgtctgct cggccgcggc ctggcgctgc tgacgttcgt
     2101 catgctggcg tcggtcgcgt tcgccgtggc cggcgcgccc aacgatgggc tcgcgcctat
     2161 cccgccactg gccagcgccg tcaccgacca gaccggaacg ctgtcggctt ccgagcgccc
     2221 ggcgctcgag gccaagctgc gcgactggga agctcgcacc accaaccagc tggcggtgct
     2281 gatcgtgccg accactgcgc cggagccgat cgagcagtac tcgatccgcg tcgccgaggc
     2341 ctggaagatc gggcgcaagg gcgtggacga cgggcttctt ctgatcgtcg cgaagaatga
     2401 ccgccgcctg cgcatcgaag tg
//
LOCUS       DATANS010000803         2515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_502325, whole genome shotgun sequence.
ACCESSION   DATANS010000803 DATANS010000000
VERSION     DATANS010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502325"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VHT22_11940"
     CDS             complement(<1..766)
                     /locus_tag="VHT22_11940"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEX3138316.1"
                     /translation="MLGRLVDDRCRRLIGGAVVAACCLFVPVAGAGDGATTAGTPPSI
                     DAEKFFSALVKVRTHALPDARSAATLGTDREGTGIVIDDKGLILTIGYLIVEADQVDI
                     VDDQGHTLPARVVGYDHSSGLGLVRPLAPLKASPLPLGDSGKLAETDPVLIVNHGGRE
                     QATLAYVVSRRPFIGSWEYLIDQAIYTSPPTLNWSGAALIGKDGKLLGVGSLILRDAT
                     EADPHLPGNMFVPIDLLKPILSEMVQTGHRSGPARPW"
     gene            959..>2515
                     /gene="araD"
                     /locus_tag="VHT22_11945"
     CDS             959..>2515
                     /gene="araD"
                     /locus_tag="VHT22_11945"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HEX3138317.1"
                     /translation="MSATRKKPEELRSHRWFGSDDMRQFGHHSRIAQMGYSRADYIGK
                     PIIAVINTWSDINACHSHFRQRAEEVKRGVLQAGGFPLEMPAIALAEVFQKPTTMLYR
                     NLLALETEELLRSYPVDGAVLMGGCDKTTPALLMGAISMNLPAIFMPAGPMLSGHWRD
                     QELGSGSDVWKYWAEKRAGNISDRQWLELQDGIARSPGHCMTMGTASTMTSAAEALGF
                     TRPGAASIPAADSRHGRMATDTGRRIIDMVWSDLKPRDFITAGSVDNAITTALALGGS
                     TNSVVHLIAIARRAGVPLTLDRFDALARKTPLVANIRPAGAHLMEDFFYAGGLRALLA
                     GIADLLSVDERTVNGKTLGENIAGAEVFNADVILPRDKALVSGSSLAVLRGNLAPDGA
                     VIKPAAAEARLLKHTGPAVVFADYNDMAARIDDPALSVTADSVLVLQNAGPLGAPGMP
                     EWGQLPIPKKLLAQGIRDMVRISDARMSGTSYGACVLHVAPESFAGGPLALVRDGDLI
                     ELDVDARKLEL"
BASE COUNT          450 a          801 c          848 g          416 t
ORIGIN      
        1 gccagggccg agcggggccg gaacggtgtc cggtctggac catctccgaa aggatcggct
       61 tcaacagatc gatcggcacg aacatgttgc ccggcagatg cggatcggcc tcggtcgcgt
      121 cgcggaggat cagtgagccg acgccgagca gcttgccatc cttgccgatc agcgcagcgc
      181 cgctccaatt gagcgtcggc ggcgaggtgt aaatcgcctg gtcgatcagg tattcccagc
      241 tgccgatgaa tggacgccgc gaaaccacat aggccaatgt cgcctgttcg cgtccgccgt
      301 gattgacgat cagaaccggg tccgtttcgg caagcttgcc cgagtcgcca agcggcaacg
      361 gcgatgcctt cagcggcgcc agcggccgca cgagtccgag cccgctggag tgatcgtagc
      421 cgacgacccg cgccggtagc gtatggcctt gatcatcgac gatgtcgacc tggtccgctt
      481 cgacgatcag atagccgatc gtcaggatca gccccttgtc gtcaatgacg ataccggttc
      541 cctcgcgatc ggtgcccagc gtagcggcgc tgcgcgcgtc gggcagcgcg tgggtccgca
      601 ccttgaccag ggcggagaag aatttctcgg cgtcgatgct tgggggggtg cctgctgtgg
      661 tggcgccgtc gccagcaccc gcgacgggga cgaacaggca acaggcggca acgacagcgc
      721 cgccgatcag ccggcggcat cgatcatcga ccaaccgtcc gagcatcggc ggcctccttg
      781 agatacgcgg cccgtggcgg cgccgttgcg atcgccgggt cgcagtggca ccgggattcg
      841 cttcgcggcc cgcgtacgat tacagcgatt tgaccgtgac tacagtagca gggttccggt
      901 cgctataatg cgggcacttc gccgttctgt ccggagaaaa tccattgaag ggcacaaagt
      961 gagcgcaacg cgcaagaaac ccgaggagct ccgctcgcac cgatggttcg gctccgatga
     1021 catgcgccag ttcggacacc attcgcgcat cgcgcaaatg ggctattcgc gggccgacta
     1081 catcggcaaa ccgatcatcg ccgtcatcaa cacctggagc gatatcaacg cctgccattc
     1141 gcatttcagg cagcgcgccg aggaggtcaa gcgcggcgtg ctgcaggccg gcggcttccc
     1201 gcttgagatg ccggcgatcg cgctggccga ggttttccag aagccgacga cgatgctgta
     1261 ccgcaatctg ctcgcgctgg aaaccgagga gctgcttcgc tcgtacccgg tcgacggcgc
     1321 cgtgctaatg ggcggttgcg acaagacgac gccggctttg ctgatgggag cgatcagcat
     1381 gaacctgcct gcgatcttca tgccggcagg cccgatgctt tccggccact ggcgcgacca
     1441 ggagttgggc agcggttcgg atgtatggaa atactgggcg gaaaaacgcg ccggcaacat
     1501 cagtgaccgg caatggctcg agctccagga cggcatcgcc cgttcgccag gacactgcat
     1561 gacgatgggc acggcatcga cgatgacgtc ggccgccgag gcgctggggt tcacccggcc
     1621 cggtgcggca tcgattcccg ccgcggattc gcggcacggc aggatggcca cggataccgg
     1681 acgacgcatc atcgacatgg tgtggtcgga tttgaagccg cgcgacttca tcaccgccgg
     1741 aagcgtcgac aacgcgatca cgacggcgct ggcgctcggg ggatcgacca attcggtcgt
     1801 gcacctgatc gccatcgccc gccgcgccgg cgtgcctttg acgctcgacc gattcgacgc
     1861 gctggcgcgc aagacgccgc tggtggccaa catccggccg gcaggcgcgc acctgatgga
     1921 ggatttcttc tacgccggcg gcttgcgcgc gctgctggcg ggcatcgccg atttgctgtc
     1981 ggtcgacgag cgcacggtca acggcaaaac gctgggggaa aatatcgccg gcgccgaggt
     2041 gttcaatgcc gatgtgatcc tgccgcgcga caaggcactg gtatcgggaa gcagtctggc
     2101 ggtgctgcgc ggcaacctcg ctccggatgg cgcggtgatc aagccggcgg cggccgaggc
     2161 gagactgctc aagcacaccg gacccgcggt cgtcttcgcc gactacaacg acatggcagc
     2221 gaggatagac gatccggcgc tctcagtaac cgccgattcg gtgctggttc tgcagaatgc
     2281 cggtccgctg ggcgcgcccg gcatgcccga gtggggccag ctgccgatcc cgaaaaaact
     2341 gctggcgcaa ggcatccgcg acatggttcg gatctccgac gcgcgcatga gcggaacgag
     2401 ctacggtgca tgcgtgctgc atgtcgcgcc ggaatcgttt gccggcggcc cgcttgcgct
     2461 ggtgcgcgac ggggatctga tcgagctcga cgtcgacgcg cgcaagctcg agctc
//
LOCUS       DATANS010000804         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_502504, whole genome shotgun sequence.
ACCESSION   DATANS010000804 DATANS010000000
VERSION     DATANS010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502504"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VHT22_11950"
     CDS             complement(<1..623)
                     /locus_tag="VHT22_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3138318.1"
                     /translation="MISLEARSEPSRLMAWASPLLAAVATLVAGFVLFSLLGKNPVDA
                     FFVFFIEPVATRYGIGELLLKSSPLMLCALGLAIGYRANVWNIGAEGQLTIGAICGGG
                     LALWLPQLNGALLLPLMIAAGAAGGIAWAAIPAWLRTRFNANEILTSLMLVYIAVLAL
                     SYLVQEPWRDPEGFNFPQSSLFGDNAMLPLLLPGTRLNVGFLLAVAV"
     gene            complement(623..2179)
                     /locus_tag="VHT22_11955"
     CDS             complement(623..2179)
                     /locus_tag="VHT22_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3138319.1"
                     /translation="MDAAPVPRLQLSGITKRFPAVVANDGIDLTVMPGEIHAVLGENG
                     AGKSTLVKIVYGVVRPDAGTIRWNGEVLVVDSPAKARALGIGMVFQQFSLFETLTVAE
                     NIALALDKSRVGRELDTRIRETSTRYGLALDPRRHIHTLSVGERQRVEIVRCLLQTPE
                     LLIMDEPTSVLTPAAIGTLFGTLRRLADEGCSILYISHKLDEIRTLCDRATVLRAGRV
                     TGNCDPRRQSSADLARMMIGHDLPHPQHRAAAARGDGLLEVRNLSLAAEDPFGTDLRG
                     IDLRVGGGEIVGIAGISGNGQKELIAALAGERLIDAADAIVVNGIPAGQLDAAARRAL
                     GLCYVPEERDGHGAVPEMSLEENALLTAYGQQLVTRWGLIRQRAVGAFAQATIARFDV
                     RTAGPGAAARSLSGGNLQKFIVGREILQMPRVMIAAQPTWGVDIGASAEIRQALIDLR
                     DAGVAVLLVSEELEELFEVCDKVAVMTQGRLSPVRAIRETDAEELGLLMGGSFVTSGQ
                     PPSFATRDPP"
BASE COUNT          382 a          750 c          741 g          350 t
ORIGIN      
        1 atgaccgcga cggcgaggag gaagcccacg ttcaggcgcg tgccgggcaa cagcagcggg
       61 agcatcgcgt tgtccccgaa caggctcgac tgcggaaagt tgaacccctc gggatcgcgc
      121 cacggttcct ggaccaggta cgacagcgcc agcacggcga tgtagacgag catcaggctg
      181 gtgagaatct cgttggcgtt gaagcgcgtg cgcagccacg ccggaatcgc ggcccaggcg
      241 atgccgcctg cggcgcctgc ggcgatcatc agcggcagca gcagcgcgcc gttcaactgc
      301 ggcaaccaca gcgccaggcc gccgccgcat atcgcgccta tcgtcaactg gccttcggcg
      361 ccgatgttcc atacgttggc ccggtatccg atcgcgaggc cgagcgcgca cagcatgagc
      421 ggcgacgact tgagcagcag ctcgccgatg ccgtaccgcg tggcgacagg ttcgatgaaa
      481 aagacgaaga acgcgtcgac gggattcttg ccgagcagcg aaaagaggac aaagccggcg
      541 acgagggttg ctacggcggc cagcagcggt gacgcccagg ccatcagtcg cgacggctcg
      601 gaccgcgcct ccaacgaaat catcatggcg ggtcgcgtgt cgcgaacgag ggcggctgcc
      661 ccgaggtgac gaacgatccg cccatcagca gcccgagttc ctcggcgtcg gtttccctta
      721 tcgccctgac cggcgacagg cgaccctgcg tcatcacggc gaccttgtcg cagacctcga
      781 acagctcctc cagctcctcg gatacgagca gcacggccac acccgcatcg cgcaggtcga
      841 taagcgcctg ccggatctcg gccgaggccc cgatgtcgac gccccaggtc ggctgtgcgg
      901 caatcatcac gcgcggcatt tgcagaatct cacgtccgac gatgaatttc tgcagattgc
      961 cgccggacag gctgcgcgcc gccgccccgg gaccggcggt cctgacgtcg aacctcgcga
     1021 tcgtcgcctg cgcgaacgct cctactgcgc gctgccgtat caggccccac cgcgtgacca
     1081 gctgctgtcc gtaggcggtc agcagtgcgt tttcttcgag cgacatctcc ggcacggcac
     1141 cgtggccatc gcgctcttcg ggaacatagc aaagtcccag cgcccggcgc gcggccgcgt
     1201 cgagttggcc cgccggaatc ccattgacga cgatcgcgtc cgccgcatcg atgaggcgct
     1261 cgcctgccag cgcggcaatc agttccttct ggccattgcc ggaaatcccg gcaatgccaa
     1321 cgatctcgcc gccgccgact ctgagatcga tgcccctgag atcggtgccg aaagggtctt
     1381 cggcggcgag cgacagattg cgcacttcga gcagtccgtc gccgcgcgcg gccgcggcgc
     1441 ggtgctgcgg atgcggcagg tcgtggccga tcatcatgcg cgcaagatcg gcgctgctct
     1501 gccggcgcgg atcgcaattg ccggtgaccc gtccggcgcg caggacagtc gcgcggtcgc
     1561 acaaggtgcg gatctcgtcg agcttgtgac tgatgtatag gatcgagcag ccttcgtcgg
     1621 ccaggcgccg gagtgtgccg aacagcgtgc ctatcgccgc cggcgtcagc accgacgtcg
     1681 gctcgtccat gatcagcagc tcgggggtct gcagaaggca gcgcacgatc tcgacgcgct
     1741 ggcgctcgcc taccgacagc gtgtggatgt ggcggcgcgg atcgagcgcc aggccataac
     1801 gtgtggaagt ctcccgaatc cgcgtgtcga gctcgcgtcc gacgcggctc ttgtccagcg
     1861 ccagtgcaat gttttccgcg acggtgagcg tctcgaacag cgagaactgc tggaacacca
     1921 tgccgatccc cagcgcgcgc gccttcgcgg ggctgtcgac gacgagtact tcgccgttcc
     1981 agcgtatcgt gccggcgtcg ggccggacga cgccgtagac gatcttgacg agcgtcgatt
     2041 tgccggcgcc gttttcgccg agcaccgcgt ggatctcacc gggcatgacc gtgagatcga
     2101 tgccgtcgtt ggcgacgacc gccgggaaac gcttggtgat gccggaaagc tgcaggcgtg
     2161 gaaccggcgc cgcgtccatg cgtcagctgc gcagagtcgc catcgaggcg gtgcgcggat
     2221 gtt
//
LOCUS       DATANS010000805         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_377154, whole genome shotgun sequence.
ACCESSION   DATANS010000805 DATANS010000000
VERSION     DATANS010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377154"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VHT22_11960"
     CDS             complement(<1..735)
                     /locus_tag="VHT22_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX3138320.1"
                     /translation="MTIARFGGPEVLELSEVAPPAPRPGEALVELECAGVNFIDIYMR
                     SGLYARSHTYQTPLPMTLGMEGGGVVVGLGEGVADVALGDRVAYCIVRGSYAQFASVP
                     VSRLVPVPPHVPMPIATGLMLQGLTAHYLSHSAYSLGPGKTCLVHAGAGGVGQLLIQL
                     AKLRGASVIATVGSEEKAAIARERGADHLILYRNVDFRDEVRRLTDGRGVDVVYDAVG
                     RDTIARSIRSLARRGLCINYGGASGLV"
     gene            complement(741..1862)
                     /locus_tag="VHT22_11965"
     CDS             complement(741..1862)
                     /locus_tag="VHT22_11965"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX3138321.1"
                     /translation="MSDIARLDRIDVHRLRIPLVTPYRLAFGPVDHFDTIVVEITDRH
                     GRVGLGEATVLTGYTDETIDESWRVARDFASQLATMEAGVAFDAIATLGARYPFTATA
                     FGSALEMLVGNAALEVHETTRVPVLGLLNAEREEAIAAEFEQLLAAGFRTIKVKVGID
                     AVGDGERVRAVQRAVAGRARIRIDANQGYTADEGVAFVKALDPADIELFEQPCAAGDW
                     DAHLSVARVASVPMMLDESIYGIDDIDRAAELSAARYIKVKLMKLGTLERLAAAIARI
                     KALGMRAVLGNGVACDLGCWMEACVAARTIDNAGEMNGFLKARSGLLTTPLEFEDGAI
                     VLRTGFAPRLDHERIAPYRLDFAGFVRTGPVIAVRETSQ"
     gene            complement(1859..>2121)
                     /locus_tag="VHT22_11970"
     CDS             complement(1859..>2121)
                     /locus_tag="VHT22_11970"
                     /inference="COORDINATES: protein motif:HMM:NF018112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138322.1"
                     /translation="GVMDVPWSPNRHCKSRVLPARDVDGCLRILDPGLMPFPRDILEL
                     HEERLRRRAERDRIAFGPDLAVQSVYEMSEPVDKLLPDRWMR"
BASE COUNT          376 a          746 c          662 g          337 t
ORIGIN      
        1 gaccagtccc gacgcgccgc cgtagttgat acacaggccg cgtcgcgcga ggctgcgtat
       61 gctgcgcgcg attgtgtcgc ggccgactgc gtcgtagacg acatcgacgc cgcgaccgtc
      121 ggtcagacgc ctcacctcgt cgcggaaatc cacgttgcga tagagaatta ggtggtcggc
      181 cccgcgttcg cgggcgatcg ctgccttttc ttcgctgccc acggtcgcga tgacgctcgc
      241 tccacggagc ttcgccagct ggatgagcaa ctggccgacg ccacccgcgc cggcgtggac
      301 gagacaggtc tttcccggcc cgagcgaata cgctgaatgc gacagataat gcgcggtcag
      361 cccctggagc ataagcccgg tcgcgatcgg catcggcacg tgcggcggaa ccggaaccag
      421 ccgcgagacc ggcaccgaag cgaactgggc atagctgccc cggacgatgc aatacgcgac
      481 ccgatcgccc agcgcaacat cggccacacc ttcacccagt ccgaccacga cgccgccgcc
      541 ttccatgccg agcgtcatcg gcagcggcgt ctgataggtg tgcgaacgag catacagacc
      601 gctgcgcatg tagatgtcga tgaagttgac gcccgcacac tccagctcga ccagcgcctc
      661 gcccggacgc ggtgccggag gagcaacttc gctcaattcg agtacctcgg gaccaccgaa
      721 gcgggcgata gtcactgctt tcactggctc gtctcccgca cggcaatcac ggggcccgtg
      781 cgcacgaaac cggcgaagtc gaggcgatac ggcgcaatgc gctcgtgatc gagccgcggc
      841 gcaaacccgg tcctcagcac gatcgccccg tcctcgaatt cgagcggcgt cgtaaggagt
      901 ccgctgcgtg ccttgaggaa cccgttcatt tcgcccgcat tgtcgatcgt ccgcgcggcg
      961 acacacgctt ccatccagca gccgaggtcg caagctacgc cgttgccgag caccgcgcgc
     1021 atgcccagcg ccttgatgcg cgcgattgcc gccgcgagcc gctcgagcgt gccgagcttc
     1081 atcagcttca ccttgatata gcgtgcggca ctcagctccg ccgcgcggtc gatgtcatcg
     1141 atcccgtaga tcgattcgtc gagcatcatc ggcaccgaag cgacacgcgc aacggacagg
     1201 tgcgcgtccc agtctccggc cgcacacggc tgttcgaaga gctcgatgtc ggcagggtcg
     1261 agcgctttca cgaacgcgac accctcgtcc gccgtataac cctgattggc gtcgatccgg
     1321 atgcgagccc gaccagcgac ggcgcgctgc acggcgcgca cgcgctctcc gtcgccgacg
     1381 gcgtcgatgc ccactttgac cttgatcgtg cggaaacccg cggcaagaag ctgctcgaac
     1441 tcggccgcga tcgcctcctc gcgctccgca ttgagcagcc ctagcactgg gacacgggtg
     1501 gtctcgtgca cctcgagcgc agcattgccg acgagcattt cgagcgccga cccgaacgcc
     1561 gtcgccgtga acgggtatcg agcgcccagc gtggcgatgg catcgaacgc gacaccggcc
     1621 tccatggtcg ccagttgcga cgcgaagtct cgcgccacgc gccagctctc gtcgatcgtc
     1681 tcgtcggtat acccggtcag caccgtggct tcgcccaggc cgacgcgtcc atggcgatcg
     1741 gtgatctcga cgacgatcgt gtcgaaatgg tcgaccggac cgaaagcgag ccggtacggc
     1801 gtgaccagcg ggatgcggag ccggtgaacg tcgatgcggt cgagtcgcgc gatgtcgctc
     1861 atcgcatcca ccgatccggc agcagcttgt cgaccggctc cgacatttcg tagacgcttt
     1921 gcaccgccag atccggtccg aacgcgatcc ggtcgcgctc cgcgcgcctg cgcagacgct
     1981 cctcgtgcaa ctcgaggatg tcgcgtggaa aaggcatcag cccagggtcg agaatgcgca
     2041 ggcatccgtc gacgtcgcgc gccggcagca cacggctctt gcaatgccgg ttcggcgacc
     2101 acggcacgtc catgacgccg g
//
LOCUS       DATANS010000806         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_755706, whole genome shotgun sequence.
ACCESSION   DATANS010000806 DATANS010000000
VERSION     DATANS010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755706"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VHT22_11975"
     CDS             complement(<1..1259)
                     /locus_tag="VHT22_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX3138323.1"
                     /translation="MSNKPNDYKSTLNLPDTVFPMRGDLAKREAAWVREWQERKVYEA
                     IRNASRGRPRFVLHDGPPYANADIHIGHAVNKILKDIVVKSKGLAGFDAPYVPGWDCH
                     GMPIEVQIEKTHGKNIPVEETQRLARAYATEQIARQKAQFQRLGVLGDWDHPYTTMAF
                     RNEADEMRMLGAILAKGYIYRGLKPVNWCFDCQSALAEAEVEYEDRTDIAIDVGFEVD
                     PRDRAALARAFGLGRLPDGPARAVIWTTTPWTIPANQALAVHPDLVYSLVETDRGSLI
                     LATELVDACLARFGIARREPAGRFEHKSSGRSLEGIRFRHPFYDRESPVQLGEFVTLE
                     QGTGIVHSSPAYGVDDFQSCRRYGLTDDQILNPVQGDGRYAATLPFFGGLNIWKANPL
                     IVEKLREVGALLHAEKVSHSYMHCWRH"
     gene            complement(1262..>2113)
                     /locus_tag="VHT22_11980"
     CDS             complement(1262..>2113)
                     /locus_tag="VHT22_11980"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX3138324.1"
                     /translation="QAMLARLSEAAEDLALPSAVLTFDPHPREFFAPGAAPPRLSTLR
                     SKLEQFAAHGIAEVHVARFNAALAALPAERFIEDVLMHRLGTRWALVGDDFRFGRGRS
                     GDLALLRRAAKTFSVEAMPTVVVDGERASSTAIRAALAAGELDHAARLLGRPYAIGGK
                     VVHGDKLGRSLGFPTANIVLRHPPPLTGVFAVRVHGLGGAARAGAASLGVRPTVKAGA
                     KPLLEVFVFDFDEAVYGRRIVVEFLRKLRDEERYADLDTLTRQIHADVAQAREYFATG
                     SSALARE"
BASE COUNT          363 a          707 c          676 g          367 t
ORIGIN      
        1 ttgtgccgcc agcaatgcat gtagctgtga ctgacttttt cggcgtgcag cagcgcaccg
       61 acctcgcgca gcttttcgac gatcagcgga ttggctttcc agatgttgag gccgccgaag
      121 aacggaagtg tggccgcgta acggccgtca ccctgcaccg gattcaatat ctggtcatcg
      181 gtgagaccgt agcgccggca cgactggaaa tcatcgacac cgtacgccgg cgagctgtgc
      241 acgatgccgg tcccctgctc cagggtgacg aattcgccaa gctgaaccgg cgattcgcgg
      301 tcgtagaacg ggtggcgaaa acgaatgccc tcaagcgatc gaccggacga cttgtgctcg
      361 aaccggccgg cgggctccct tcttgcgatg ccgaagcgcg ccaagcaggc atcgaccagc
      421 tcggtcgcca ggatgaggct ccctcgatcg gtctcgacca gcgaatagac gaggtcgggg
      481 tgcacggcca gcgcctgatt ggccgggatg gtccacggcg tcgtcgtcca gatgaccgcg
      541 cgcgccggac catcgggcaa tcgcccgagg ccgaatgcgc gcgccagcgc cgcccggtcg
      601 cgaggatcaa cttcgaagcc gacgtcgatc gcgatatcgg tgcggtcctc gtactcaact
      661 tcggcttccg ccagcgccga ctggcagtcg aagcaccagt tcaccggctt gaggccgcga
      721 taaatgtagc ctttggccag aatcgcgccg agcatgcgca tctcatcggc ttcgttgcga
      781 aacgccatcg tcgtgtacgg atgatcccag tcaccgagca cgcccagccg ctggaactgc
      841 gccttttgcc gcgcgatctg ctcggtcgca tacgccctcg ccagccgctg cgtctcctcg
      901 accgggatgt tcttgccgtg cgttttctcg atctgcactt cgatcggcat gccatggcaa
      961 tcccagccag gcacataggg cgcgtcgaag ccggccaggc ccttgctctt gacgacgatg
     1021 tccttgagga ttttgttaac cgcgtgcccg atatggatgt ccgcgttcgc atacggcggg
     1081 ccgtcgtgca gaacaaaccg cggccggccg cggctggcgt tgcggatcgc ttcgtagacc
     1141 ttccgctcct gccattcgcg aacccaggcg gcttcccgct tcgccaggtc gccgcgcatc
     1201 ggaaataccg tgtcgggcag attgagcgtc gacttgtagt cgttgggctt gttggacatc
     1261 gttactctct cgcgagcgcg ctcgaacccg tcgcgaaata ctcgcgcgcc tgcgctacgt
     1321 cggcatgaat ctgtcgcgtc aacgtgtcga ggtcggcgta ccgctcctcg tcgcgcagct
     1381 tgcggagaaa ttcgacgacg atgcggcggc catataccgc ttcatcgaaa tcgaacacga
     1441 aaacctccag cagcggcttc gcccccgcct tgactgtcgg ccgcacgccg aggctggcgg
     1501 cgcctgctcg cgcagcgccg cccagaccgt gcacgcgcac ggcgaaaacc cccgtcagcg
     1561 gcggcggatg gcgcaacacg atgttggccg tcggaaagcc gaggctgcgt cccagcttgt
     1621 cgccgtggac gaccttgccg ccgatcgcat acggtcgtcc gagcaatctc gccgcgtgat
     1681 cgagctcgcc tgcggcgagc gcggcgcgta tggccgtgct ggacgcccgc tcgccgtcga
     1741 cgacgaccgt gggcatcgcc tcgacgctga acgtcttcgc ggctcgccgc agcagcgcaa
     1801 gatcgccgct tcgcccacgg ccgaagcgaa aatcgtcgcc gaccagcgcc cagcgcgtgc
     1861 cgagacggtg catcagaacg tcttcgatga agcgctccgc tgggagcgca gccagcgcgg
     1921 cgttgaagcg cgcgacatgg acctcggcga tgccgtgtgc agcgaattgc tcgagcttgc
     1981 tgcgcaaggt ggacaggcgc ggcggcgcgg cgccgggtgc gaaaaattcg cgcggatgcg
     2041 ggtcgaaggt caggacagcc gacggcagtg ccagatcttc cgcagcctcg gacaaccggg
     2101 cgagcatcgc ctg
//
LOCUS       DATANS010000807         3188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_625825, whole genome shotgun sequence.
ACCESSION   DATANS010000807 DATANS010000000
VERSION     DATANS010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625825"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2074)
                     /locus_tag="VHT22_11985"
     CDS             complement(<1..2074)
                     /locus_tag="VHT22_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX3138325.1"
                     /translation="MSAADKTIHVCSCNGTMPLDAAALARVLDLAGPLPLHTELCQKE
                     LARFADRAEGDLLVACTQEARVFGDVAEDSGKTQTIRFVNIRENAGWSAEARAATPKI
                     AALLAMAALPEPQPVPRVAFKSEGRLLIVGPAGAALYWAEALKDRLDVTVLITGRAIG
                     NELPPERDYPICSGKLTRLSGWLGEFSAQWVQENPIDLDLCTRCNACIRACPEEAIDA
                     SYQIDLDRCRDHRQCVIACGAIGAIDFDRTAAQTAREESFDLVLDLQRKPAFAMHQPP
                     QGYWHPGDDAAAQAMVIVDVLGAVGEFEKPKYFTYKASICAHSRSQQPGCNLCIDICS
                     TAAIRPDGDHIAVEPHLCMGCGACTTVCPSGALGYAYPAVPDLGSRVRTLLATYAKAG
                     GRDACLLFHDAAGAEVIHRLGRRGKGLPARVIPVELHHMAAAGIDTWLGALAYGASDV
                     AVLATGTEAPQYPEAIERQMGYADTIAQALGYQGRHLHLLRAADAAALEAAVWALQPA
                     LPVRAPATFHWSADKRTTAALAIEHLQAHAPTPQTVIALAAGAPFGTIAVNRDACTLC
                     LACVGACPEGAILDHPASEVPQLRFIESKCVQCGLCEATCPEDAIALTPRLSLVAEAK
                     QPRVLNEASIFKCIRCGKALGTDKMIDNMLTKLAGHSMFAAPGSLDRLKMCADCRVID
                     MMQKELNVA"
     gene            complement(2071..2706)
                     /locus_tag="VHT22_11990"
     CDS             complement(2071..2706)
                     /locus_tag="VHT22_11990"
                     /inference="COORDINATES: protein motif:HMM:NF023176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HEX3138326.1"
                     /translation="MNDKPDNIAQQPERLSLKRWSRRKLEAARAETAPRDAPSEPVAT
                     AAPPPQPAHAAGPVPQNAEAPLPPIESLTIDSEFAAFFKPGVAEATKRAALKQLFRDP
                     RFNIMDGLDVYVGDYTQPDPISPEMMKQLLHTRHIFNPPKTEVNAEGHVVDVVEPTEQ
                     AEPEASPGAAAAIAISDGTGDANPGSLPGAVDESAEPDPVRQADPQGGKKA"
     gene            complement(2711..>3188)
                     /locus_tag="VHT22_11995"
     CDS             complement(2711..>3188)
                     /locus_tag="VHT22_11995"
                     /inference="COORDINATES: protein motif:HMM:NF023177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HEX3138327.1"
                     /translation="WASEKWQIGTVERDDSPLPVAVKLSDDATGTRWRFTGFGVELHA
                     SECEGYFLNITAPEPKIFVMWRMLEPERIAADGLSAKPQLVTVSYNEAARLMDGGEMV
                     DAVPLFAELLAWMQPYVAANYKPEPKRKVRRNELYERDDQHRDASARKVSDAPTKA"
BASE COUNT          467 a         1075 c         1099 g          547 t
ORIGIN      
        1 ccgcgacgtt caattccttc tgcatcatgt cgatcacgcg gcagtcggcg cacatcttga
       61 gccgatcgag cgagcccggc gcggcgaaca tcgagtgccc ggcgagcttg gtcagcatgt
      121 tgtcgatcat cttgtcggtg cccagggcct tgccgcagcg gatgcatttg aagatcgacg
      181 cctcgttcag gacccgcggc tgcttcgctt cggcgaccag cgacaagcgc ggcgtcagcg
      241 cgatcgcgtc ctcgggacag gtcgcctcgc acaggccgca ttgcacgcat ttggactcga
      301 tgaagcgaag ctgaggcact tccgaggccg gatgatcgag gatcgcgcct tccgggcagg
      361 cgccgacgca ggccaggcat agcgtgcagg catcgcgatt gacggcgatg gtgccgaacg
      421 gcgcgccggc ggcgagcgcg atcaccgttt gcggtgtcgg cgcgtgagcc tgcagatgct
      481 cgatggcgag cgcggcggtc gtgcgcttgt ccgccgacca atggaacgtc gccggcgcgc
      541 ggaccggcag cgccggctgc agcgcccaga cggccgcctc gagcgcggcg gcgtcggcgg
      601 cgcgcagcag atgcaggtgc cggccctggt agccgagcgc ctgcgcgatg gtatcggcgt
      661 agcccatctg ccgctcgatc gcttccggat attgcggcgc ttcggtgccg gtcgcgagca
      721 cggcgacgtc gctcgcgccg tacgcgagcg cgcccagcca ggtgtcgatg cctgcggccg
      781 ccatgtgatg cagctcgacc gggatcaccc gcgccggcag gcccttgccg cgacggccga
      841 ggcgatggat cacctccgcg ccggccgcat cgtggaacag caggcatgcg tcgcgcccgc
      901 cggccttggc ataggttgcg agcagcgtgc gcacccgcga cccgaggtcg ggcacggccg
      961 gataggcata acccagggcg ccggacgggc acacggtcgt gcaggcgccg caacccatgc
     1021 aaaggtgcgg ctcgaccgcg atgtgatcgc cgtcggggcg gatcgcggcc gtcgagcaaa
     1081 tgtcgatgca tagattgcat ccgggctgct gcgagcggct gtgcgcgcag atcgatgcct
     1141 tgtacgtgaa atacttgggc ttttcgaatt cgccgaccgc gcccagcacg tcgacgatga
     1201 ccatcgcctg cgctgccgcg tcgtcgcccg gatgccagta gccctgcggc ggctggtgca
     1261 tcgcgaacgc cggcttgcgc tgcagatcga gcacgagatc gaacgactcc tcgcgcgccg
     1321 tttgcgccgc ggtgcggtcg aagtcgatcg cgccgatggc gccgcatgcg atcacgcact
     1381 gacggtggtc ccggcagcgg tcgaggtcga tctggtagct ggcgtcgatc gcctcttcgg
     1441 ggcatgcgcg gatgcacgcg ttgcaccggg tgcacaggtc cagatcgatc ggattctcct
     1501 gaacccactg cgcgctgaac tcgcccagcc accccgaaag ccgggtgagc ttgcccgagc
     1561 agatcgggta gtcgcgctcc ggcggcagct cgttgccgat cgcccgaccc gtgatcagca
     1621 cggtgacgtc gagccggtcc ttcagcgcct cggcccagta caaggccgca cctgccgggc
     1681 cgacgatcaa gagccgccct tccgatttga atgcgacgcg cgggaccggc tgcggctcgg
     1741 gcagcgccgc catcgccagc aaggccgcga tcttgggcgt cgcggcgcgc gcttcggccg
     1801 accagcccgc attctcgcgg atgttgacga agcggatcgt ctgcgtcttg cccgaatcct
     1861 cggcgacgtc gccgaatacg cgcgcttcct gcgtgcaagc gaccagcaag tctccttcgg
     1921 cgcggtcggc gaagcgggcg agctccttct ggcagagctc ggtgtgcaag ggcagcggac
     1981 ccgcgaggtc aagcacgcgc gcgagcgccg cggcgtcgag cggcatcgtg ccgttgcagc
     2041 tgcagacgtg gattgtcttg tcggcggcgc tcatgctttc ttgccgcctt gcgggtccgc
     2101 ctgccgaacc gggtccggct cggctgactc gtcgactgcg cccggcagcg atcccgggtt
     2161 cgcgtcgccg gtgccgtcgg atatcgcgat cgccgctgcc gcgccgggcg atgcttccgg
     2221 ctcagcctgc tcggtaggct cgacgacgtc gacgacgtgc ccttcagcgt tcacttcggt
     2281 tttcggcgga ttgaagatgt gccgtgtatg caacaactgc ttcatcatct ccggtgaaat
     2341 cggatcgggc tgcgtgtaat cgccgacgta gacgtccaga ccgtccatga tgttgaagcg
     2401 cggatcgcga aacagctgct tcagcgccgc gcgcttggtc gcttcggcga cgccgggctt
     2461 gaaaaacgcc gcgaattccg aatcgatagt cagcgactcg atcggcggca gcggcgcctc
     2521 ggcgttctgc ggcacgggcc ctgctgcgtg cgcgggctgc ggcggcggcg ctgccgtagc
     2581 gaccggctcg gacggagcgt cgcgcggcgc cgtctcggcc cgcgccgcct cgagcttgcg
     2641 ccgcgaccag cgcttcagcg aaaggcgctc cggctgttgt gcgatgttgt ccggcttatc
     2701 gttcatcgct tcaggccttg gtcggcgcat cggagacctt gcgcgcgctt gcgtcgcgat
     2761 gctggtcgtc gcgctcgtag agctcgttgc gccgtacctt gcgcttgggc tcgggcttgt
     2821 agttcgccgc gacgtacggc tgcatccacg ccagcaattc ggcaaacagc ggaaccgcgt
     2881 cgaccatctc accgccgtcc atcaggcgcg ccgcctcgtt gtagctgacg gtgacgagct
     2941 ggggtttggc cgacaatccg tcggcggcga tgcgctcggg ctccagcatg cgccacatga
     3001 caaagatctt gggctccggt gcggtgatgt tcagaaaata accttcgcat tcggaggcat
     3061 gcagctccac gccgaatccg gtgaagcgcc agcgcgtgcc ggtcgcatcg tcggacagct
     3121 tgaccgccac cggaagcgga ctatcgtcgc gctcgacggt gccgatctgc catttttcgc
     3181 tcgcccag
//
LOCUS       DATANS010000808         3175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_247968, whole genome shotgun sequence.
ACCESSION   DATANS010000808 DATANS010000000
VERSION     DATANS010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247968"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /gene="dctP"
                     /locus_tag="VHT22_12000"
     CDS             complement(<1..683)
                     /gene="dctP"
                     /locus_tag="VHT22_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX3138328.1"
                     /translation="MRWIGRIALLVGVMCIGSGAALAQTKLKWAHVYETSEPYHKWSV
                     WAASEIEKRTSGRYHIDVFPASSLGKESDINQGLTLGTVDMIISGLSFAARTMPRIGV
                     GYYPYTFRDGDHLVKWAASPAFKEMTDEYRNKTGIQITAMTYYGVRNSTSNKPFKNCA
                     EMKGLKMRVPDVPAYLALPKACGANPTPIAFAEVYLALQNGTVDAQENPLTTIEAKKF
                     FEVQKYIILT"
     gene            complement(720..1745)
                     /locus_tag="VHT22_12005"
     CDS             complement(720..1745)
                     /locus_tag="VHT22_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HEX3138329.1"
                     /translation="MLTCRIHAKDDLRIEDSELPQAGPGQVLIRLGAGGICGSDLHYY
                     YEGRNGSFVVREPLIPGHEASGVVAKVGAGVTRVKPGDKIAVSPSHACGQCDYCREGR
                     EQLCRNMRFLGSASIYPHVQGMFSEYFVMGERQCYPVAADVSLGEIAFAEPLAVALHA
                     VNRGPELLGKSVLITGAGTIGCLTVLAARLAGARHIAVSDILDRPLATARAVGADRTI
                     RADREPDALAAPQYDVAFEVSGSFVALKACVAAAKRGGTIVQVGTIPHEPLPFVVNEI
                     MAKELDLKGAFRWGIEFDWAVDYISSRRVDVKPLLSGQFPLEDAVKAFELAKDKTRST
                     KVQVVAA"
     gene            complement(1776..2477)
                     /locus_tag="VHT22_12010"
     CDS             complement(1776..2477)
                     /locus_tag="VHT22_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX3138330.1"
                     /translation="MPIQSIEPRRLYRQIADQIRTLVRSGEYTAGQRLPPERDLARQL
                     GVSRPSVREALIALEVEGLVEVRIGSGIYVLGAGNGHDGEVQAASGPFELLRARWIIE
                     GECAALAAKSAKKEHIAAIEDALKQMQRIMETDKVQPLAADRLFHLRIAEATGNGALV
                     AVLKMLWEERMGPLFAQLEHHYDTPELWLAAITEHRSVLKAIIARNPDEARAAMQKHL
                     NKAYKRFNTGWDARQ"
     gene            2470..2634
                     /locus_tag="VHT22_12015"
     CDS             2470..2634
                     /locus_tag="VHT22_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138331.1"
                     /translation="MGMVESRAVFAGAFAEGSLQYIVAKQNTPIWSSGLTNFAARFVK
                     MLASMMKGIA"
     gene            2631..>3175
                     /locus_tag="VHT22_12020"
     CDS             2631..>3175
                     /locus_tag="VHT22_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3138332.1"
                     /translation="MSDRLAGKLALITAAGQGIGRAAALAMAKEGASVWATDVKPELL
                     EQYRGVANVSTRVLDVLDDAAVRQAIDALPPLDVLFNCAGYVHHGTVLDCTPKDWAFS
                     FDLNVRAMYVTIQCALPRMLEHCAKTGGSASIINMASVAGSLKGIPNRFAYGASKAAV
                     VGLTKSVAADFVARGIRCNAIA"
BASE COUNT          533 a         1017 c         1013 g          612 t
ORIGIN      
        1 gtgaggataa tgtatttctg cacctcgaag aatttcttcg cctcgatggt cgtgagcgga
       61 ttttcctgcg cgtcgactgt gccgttctgc agtgcgaggt aaacctcggc gaatgcgatc
      121 ggcgtcggat tcgcgccgca cgccttcggc agcgccaggt aggcgggcac atcgggcacg
      181 cgcatcttca ggcccttcat ttcggcgcaa ttcttgaacg gcttgttcga ggtcgaattt
      241 cgcacgccat agtacgtcat cgccgtgatt tggatgccgg tcttgttgcg gtattcgtcg
      301 gtcatttcct tgaatgcggg actcgccgcc cacttcacga gatggtcgcc atcgcggaac
      361 gtgtacggat agtagccgac gccgatgcgc ggcatcgtgc gcgcggcgaa cgacaggcct
      421 gagatgatca tgtcgaccgt gccgagcgtg agtccctgat tgatgtcgga ctccttgccg
      481 aggctagatg ccggaaatac gtcgatgtga tagcggccgc tagtgcgctt ctcgatctcg
      541 ctggccgccc ataccgacca cttgtggtac ggctccgaag tctcgtagac atgcgcccat
      601 ttgagcttgg tttgcgcgag tgcggcgccc gagccgatgc acatgacccc gacgagcagg
      661 gcgattctgc cgatccaacg catggtgctc ctcctgtttt atgctacggg taaaaatgct
      721 tacgcggcga cgacctgcac cttggtgctt ctggtcttgt ccttggccag ctcgaatgcc
      781 ttgaccgcat cctcaagcgg gaactgcccc gacagcagcg gtttgacgtc gacgcggcgc
      841 gacgaaatgt aatcgaccgc ccagtcgaat tcgattcccc accgaaacgc gcctttcagg
      901 tcgagctcct tggccatgat ctcgttgacg acgaacggca acggctcgtg cggaatcgtt
      961 ccgacctgca cgatcgtgcc gccgcgcttc gcggccgcga cgcaggcctt gagggcaacg
     1021 aagcttccgg aaacctcgaa cgcgacgtcg tattgcggcg ccgccagcgc atccggctcg
     1081 cggtcggcgc ggatcgtccg gtcggctccg actgcccgcg cggtcgccag cggccggtcg
     1141 agaatgtcgg agaccgcgat gtgcctggca ccggcgagac gcgcggcgag caccgtcagg
     1201 cagcctatcg tcccggcgcc ggtgatcaac accgacttgc cgagcagctc cgggccgcgg
     1261 ttgacggcgt gcagcgcaac cgcgagcggc tcggcgaacg cgatctcacc cagcgagacg
     1321 tcggcggcga ccggatagca ttgccgctcg cccatcacga aatactcgga gaacatgcct
     1381 tgcacgtggg ggtagatgct ggcgctgccc agaaagcgca tattgcggca cagctgctcg
     1441 cgtccctcgc ggcagtagtc gcattgcccg caggcatgcg acgggctgac ggcgatcttg
     1501 tctcccggct tgactcgcgt cacgccggcg ccgaccttgg cgacgacacc cgaggcttcg
     1561 tgcccgggaa tcaacggctc gcgaacgacg aagctgccgt tgcggccttc gtaatagtag
     1621 tgaaggtcgg agccgcagat gccgccggca ccgagccgga tcagcacctg gccggggccg
     1681 gcctgcggca gctcgctgtc ttcgattcgc aggtcatcct tggcgtgaat tcggcaggtc
     1741 agcatgatcg ttcctttttc gggccgggca cgcggctatt gccgcgcatc ccaaccggtg
     1801 ttgaagcgct tgtaggcctt gttcaaatgc ttttgcatcg ccgcgcgcgc ttcgtcgggg
     1861 ttgcgcgcta tgatcgcctt cagcacgctg cgatgctcgg tgatcgccgc gagccagagc
     1921 tctggcgtgt cgtagtgatg ctcgagttgc gcgaacagcg gacccatccg ttcttcccag
     1981 agcattttaa gcaccgccac cagcgcgccg ttgccggtcg cctcggcgat gcgcaggtga
     2041 aacagccgat cggcggcgag cggctgcacc ttgtcggtct ccatgatccg ctgcatctgt
     2101 ttgagcgcat cttcgatcgc ggcgatatgc tcctttttcg ccgacttggc ggccagcgcc
     2161 gcgcattcgc cttcgatgat ccagcgcgcg cgcaagagct cgaacggtcc cgaggccgcc
     2221 tgcacttctc cgtcgtggcc gtttccggcg ccgaggacgt aaatacccga gccgatgcgc
     2281 acttcgacca gcccttcgac ttcgagagcg atcagagcct cgcgcaccga cggcctggac
     2341 acgccgagct gccgcgccag atcgcgctcg ggcggaagcc gctgcccggc cgtgtattcg
     2401 cccgatcgga caagcgtgcg gatctggtcg gcgatctgcc ggtacagccg gcggggctcg
     2461 atactctgga tgggcatggt cgaaagccgc gccgtgttcg ccggcgcgtt tgcggagggg
     2521 tcgcttcaat acatcgtagc taagcaaaat acaccgattt ggtcaagtgg tcttaccaat
     2581 tttgcggccc gctttgttaa aatgttggcc tccatgatga aaggcatcgc ttgagcgacc
     2641 gactcgcggg caaattggcg ctgatcacgg ccgccggaca gggcatcggc cgcgccgcgg
     2701 cgctggcgat ggcgaaggaa ggtgcgtccg tctgggcgac agacgtcaag ccggaactgc
     2761 tcgagcagta ccgcggggtg gccaacgtca gcacgcgggt gctcgacgtc ctcgacgacg
     2821 ccgccgtgcg ccaggcgatc gatgcgctgc cgccgctcga cgtgctgttc aattgcgccg
     2881 gctacgtgca tcacggcacg gtgctcgact gcacgccgaa ggactgggcg ttcagtttcg
     2941 acctcaacgt gcgcgcgatg tacgtcacga tccagtgtgc gctgccgagg atgctcgagc
     3001 attgcgccaa gaccggggga agtgccagca tcatcaacat ggcctcggtc gccggctcgc
     3061 tcaaaggcat tccgaatcgc tttgcctacg gcgccagcaa agccgccgtc gtcggactga
     3121 ccaagtcggt cgcagccgat ttcgtggcca ggggaatacg ctgcaacgcg atcgc
//
LOCUS       DATANS010000809         3629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_836312, whole genome shotgun sequence.
ACCESSION   DATANS010000809 DATANS010000000
VERSION     DATANS010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836312"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..888)
                     /gene="cmk"
                     /locus_tag="VHT22_12025"
     CDS             complement(187..888)
                     /gene="cmk"
                     /locus_tag="VHT22_12025"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX3138333.1"
                     /translation="MTSDSKADAAPVIAIDGPAASGKGTIAQGVAVALGYRYLDSGSL
                     YRLVALRALRQKVAFDDEPRLAALADGLDLSFADGRTFLSGQDVSEAIRAEEVSAMAS
                     KVAVLPRVRTALLTRQRAFRAPPGLVAEGRDMGTVVFPEAKLKIYLTASAEARASRRH
                     KQLIDKGNSITIQGLLRDIRERDARDSARAEAPLKTAADAMVLDTTDMAADAAIAFVL
                     QQYQALSTAGSTRRA"
     gene            complement(903..2216)
                     /gene="aroA"
                     /locus_tag="VHT22_12030"
     CDS             complement(903..2216)
                     /gene="aroA"
                     /locus_tag="VHT22_12030"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX3138334.1"
                     /translation="MSVSASDQRSAEFLDIAPIVRMSGTLRLPGSKSISNRSLLLAAL
                     AQGTTELTGLLDADDVDRMRESLRTLGVRIEGDVETAIVHGVAGAFPVRRASLFLGNA
                     GTALRPLTATLALSHGDYELSGTPRMHERPIGALVDALRALGADIRYTGAAGFPPLTI
                     APGAVRGGGKVAIRGNVSSQFVTALLMALPLAGGDATVEVQGELISRPYVDMTLALMR
                     RFGVAVVPERDDLFRVPAAAAYISPGTLSVEGDASSASYFLAAGAIGGGPVRIEGVGR
                     DSIQGDAAFAETLSAMGADVSSGDDWIEVRRKAKLHGIDADFNAIPDAAMTAAMVALF
                     AEGPTLLRNIGSWRVKETDRIAAMARELRKLGASVDEGADWMRVTAPAQWQPATIATY
                     DDHRIAMCFSLAALGGVPVRINDPQCVRKTFPGYFAAFERLARRP"
     gene            complement(2206..3111)
                     /locus_tag="VHT22_12035"
     CDS             complement(2206..3111)
                     /locus_tag="VHT22_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX3138335.1"
                     /translation="MSSSAPGKLVVVGTGLIGGSFALGLKEVGKVGSVVGVGRGRGNL
                     DQALRLGVIDRAYTRDEGWTAELADAVLVLLATPVGEMPALFAAIAPHLGPGTIVSDA
                     GSTKQDVIASARTTLGSVFSRFVPAHPIAGSERSGAAAASASLFRGRQVVLTPLPETD
                     PSAVERVREWWTQCGGIVTLLTAERHDALLSAVSHLPHLLAFALIGEIASRPDAADYW
                     RVAGTGLRDFTRLAESHPDMWRDICIANAVRLRADLAAYQQELSRIDATLARGDGAAL
                     GDLFVRAGAARAALLAGRRGGDDVG"
     gene            complement(3111..>3629)
                     /locus_tag="VHT22_12040"
     CDS             complement(3111..>3629)
                     /locus_tag="VHT22_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3138336.1"
                     /translation="NEYLQTDQQANSASWIAAHPNLLVSRTFSKAHGLAGLRVGYGIA
                     DASVADMLNRVRQPFNVNSIAQAAALAALADEDYVAESARLNRDGLAQLMGGLDSIGV
                     AYLPSHGNFLLVRVADAGKGAAPIYESLLRSGVIVRPVANYGLPDWLRVTVGLPEENA
                     RFLSALAAALGR"
BASE COUNT          615 a         1265 c         1196 g          553 t
ORIGIN      
        1 gtcatgtatc gtggtgtcct gtcgtttcgt ccgtcgccac gggcgtgact acgggtccgt
       61 tggtcaaacc gccgtccgga ctgccctgac tcgcgcccgg atggcggacc ttgctgtcgc
      121 cgcgagacgc ctgtagcgcc cggctggcca atctgctgcg gtactgctaa ccctgtttcg
      181 atgcgctcac gcgcgcctag tggatccggc tgtgctaagc gcctgatact gctgcaagac
      241 aaaggcgatg gccgcatcgg cggccatgtc ggtcgtatcc aggaccatcg cgtcggccgc
      301 ggtcttgagc ggtgcctccg cgcgggcact gtcgcgcgcg tcccgctccc ggatatcgcg
      361 caaaagacct tgaattgtaa tcgagtttcc tttgtcgatc aactgcttat gccggcggct
      421 cgcccgggcc tcggcgctcg ccgtcaggta gatcttcaac ttcgcctcgg gaaacacgac
      481 tgtgcccatg tctcgcccct cggccaccag ccccggcgga gcgcggaagg cccgttgccg
      541 ggtcagcagc gcggtccgca cacgcggcag gacggcgacc ttggaggcca tcgcactgac
      601 ctcttcggcg cgtatcgcct cgctaacatc ctgaccggac aagaaagtcc gcccgtctgc
      661 aaagctcaag tcgagtccgt cggcaagcgc ggcgagccgc ggctcgtcat cgaaagccac
      721 cttctgccgc aacgcccgca acgccaccag ccggtaaagc gagccgctgt cgagatatcg
      781 gtaaccgagc gccacggcga cgccctgcgc gatcgtgccc ttgcccgatg cagcagggcc
      841 gtcgatcgcg atcaccggcg ctgcgtcggc ctttgaatcg gaggtcatcg cgcggccgca
      901 gctcacggtc gccgcgccag gcgctcgaag gcggcgaaat aaccggggaa cgtcttgcgc
      961 acgcattgcg gatcgttgat gcgcaccggc acgccgccca gcgcggcaag cgaaaaacac
     1021 atcgcgatgc ggtggtcgtc gtaggtcgct atcgtcgccg gctgccattg cgccggcgcc
     1081 gtcacgcgca tccaatcggc gccttcgtcg accgaggcac ccagcttgcg cagctcgcgg
     1141 gccatcgctg cgatgcgatc ggtttccttg actcgccagc tgccgatatt gcgcagcaag
     1201 gtcgggcctt cggcgaaaag ggcgaccatc gccgccgtca ttgccgcgtc gggaatcgcg
     1261 ttgaaatcgg cgtcgatgcc gtgcagcttt gccttgcggc gcacctcgat ccaatcgtcg
     1321 ccgctgctga cgtcggcacc cattgccgaa agcgtctcgg caaaggcagc gtcgccctgg
     1381 atgctgtccc ggccgacccc ctcgatgcgc accggtccgc cgccgatcgc tccggcggca
     1441 agaaaatacg atgccgacga ggcatcgcct tcgaccgaca gcgtccccgg gctgatatac
     1501 gcagcggcgg cgggcacgcg gaacagatcg tcgcgctcgg gcacgacggc gacgccgaag
     1561 cgacgcatca gcgccagcgt catgtcgacg taaggacgcg aaatcagctc gccctgcacc
     1621 tcgaccgtcg catcgccacc ggccagcggt aacgccatca gcagcgccgt cacgaactgg
     1681 ctggatacgt tgccgcgaat cgcgaccttt ccgccgccgc gcaccgcgcc gggagcgatc
     1741 gtcagcggcg ggaatccggc ggcgccggta tagcgaatgt cggccccgag cgcacgcagc
     1801 gcgtcgacca gggcgccgat cggccgctca tgcatgcgcg gcgtcccgga caattcgtag
     1861 tcgccatgcg acagggccag cgtcgccgtc aacgggcgca aggctgtgcc cgcattgccc
     1921 aggaagagac tcgcgcgccg caccggaaac gcgccggcaa cgccatggac gatggcggtc
     1981 tcgacgtcgc cttcgatgcg cacgccgagc gtgcgcaagg actcgcgcat ccgatcgacg
     2041 tcgtccgcgt cgagcaatcc ggtgagctcg gtcgtccctt gagcgagcgc cgccagcagc
     2101 aggctgcggt tcgaaatgct tttggagccc ggcaggcgca aggtcccgga catgcgcacg
     2161 atcggagcga tgtcgagaaa ttcggcggag cgctggtccg aggcgctaac cgacatcgtc
     2221 gcctccgcgc cggccggcaa gcagggccgc gcgcgccgcg ccggcgcgca cgaacaggtc
     2281 gcccagcgcg gcgccatcgc cgcgcgcgag cgttgcatcg atgcgcgaaa gctcctgctg
     2341 gtacgcggcc aaatcggcgc gcagccgcac ggcattggcg atgcatatgt cgcgccacat
     2401 gtcggggtgg ctttccgcca accgcgtgaa atcgcgcagc ccggtcccgg cgacgcgcca
     2461 gtaatcggcg gcgtcgggcc ggctcgcgat ctcgccgatc aacgcgaatg ccagcagatg
     2521 gggcagatgg ctgaccgccg aaagcagcgc atcgtggcgc tcggcagtaa gcagggtgac
     2581 gatcccgccg cactgcgtcc accattcgcg cacgcgttcc acggcggagg gatcggtttc
     2641 cggcagcggc gtcaacacga cctggcggcc gcgaaacagc gatgccgacg cggcggcggc
     2701 acccgagcgt tcgctgcccg ctatcgggtg tgccggaacg aaacgcgaaa acacgctgcc
     2761 cagcgtcgta cgcgccgagg cgatcacgtc ctgcttggtg ctgccggcgt cgctgacgat
     2821 cgtcccgggt cccaaatgcg gcgcgatcgc agcgaacagc gcaggcattt cgccgaccgg
     2881 tgtggccagc agcaccagca cagcgtcggc gagctcggcc gtccagccct cgtcccgcgt
     2941 atacgcccga tcgatgacgc ctaagcgcag cgcttggtcg agattgccgc ggccacgccc
     3001 gacaccgacg acgctcccga ccttgccgac ctctttcagg ccaagcgcga acgagccgcc
     3061 aatcagcccc gtgccgacga cgacgagctt gcctggcgca gatgaggaca ctatcggccg
     3121 agcgcggcgg cgagcgccga gagaaagcgg gcgttctctt cgggcaatcc tacggtgacc
     3181 cgcagccagt cgggcagacc gtagttggcc accgggcgca cgatgacgcc ggagcgaagc
     3241 agcgactcgt agatcggcgc tgcgcccttt cccgcgtcgg cgacgcgaac cagtaggaag
     3301 tttccgtgcg agggcagata ggcgacgccg atcgaatcga ggccgcccat tagctgagcg
     3361 agcccgtcgc gattcaggcg cgcgctttcg gcgacatagt cttcatcggc gagcgcggcc
     3421 agcgcggctg cctgagcgat cgagttgacg ttgaacggct gccggacacg gttgagcatg
     3481 tcggcaacgc tggcatcggc gatgccatag ccgacgcgca gccctgcaag cccatgagct
     3541 ttggaaaaag tgcgtgacac cagcaggttc ggatgcgccg cgatccagga cgcgctgttc
     3601 gcctgctggt cggtctgcag gtattcgtt
//
LOCUS       DATANS010000810         1628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_335529, whole genome shotgun sequence.
ACCESSION   DATANS010000810 DATANS010000000
VERSION     DATANS010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335529"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="VHT22_12045"
     CDS             <1..1189
                     /locus_tag="VHT22_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX3138337.1"
                     /translation="IRTDRDAPRQKVVAIDLRDPRVANWKTLVAEGKDAIRKGDFIGG
                     RIVLEYLTDARSRLAVFDRAGRPQGEITLPEVGTVTEIRGREDSPTIYYSFTSALYPT
                     TVFAYDPRTRKSTPFDPPPRVADASRYESRQFFATSKDGTRVPFTVTARKDLPRDGNN
                     PTLLLGYGGFSLSQMPFYRPHVVAWLELGGVWVSSNIRGGGEYGDDWYRAARAEKRQN
                     GFDDFIAVAEHLVREKFTAPSRLAILGGSNGGLLVATVSQQRPDLYAVAMPAVAVTDM
                     LRFDKFTGGRAWTLEYGSPSDPVQFMQLIKYSPLHNVKPGTCYPATFVTTADHDDRVV
                     PSHSYKYAAEMQAGQGCSRPVLVRVEPKASHGYRPTDRLIAEIADQWTFAAEHTGMRA
                     AKP"
     gene            1186..>1628
                     /locus_tag="VHT22_12050"
     CDS             1186..>1628
                     /locus_tag="VHT22_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP transhydrogenase subunit alpha"
                     /protein_id="HEX3138338.1"
                     /translation="MTIKTIAIVGAGPGGFRLVANLGLMGYRLRLNDIDEAKLTAIRA
                     RGGIDVEGTPGGFAPLELATTDLRATVDGADLIIVVSGGNTQPTVARALAPLLVDGQT
                     ILLMQGNTGGSLVVRRELDGAGCKAKIDLAETDTYPYAMRAIGPTT"
BASE COUNT          300 a          554 c          502 g          272 t
ORIGIN      
        1 catccgcacg gaccgcgacg cgccgaggca aaaagtggtc gcaatcgacc tgcgcgatcc
       61 gcgcgttgcc aactggaaaa cgctcgtcgc ggagggcaaa gacgccattc gcaaaggcga
      121 tttcatcggc ggccgcatcg tgctcgaata cctgaccgac gcgcgcagcc gtcttgccgt
      181 gttcgatcgc gcgggccgcc cgcaaggcga gatcacgctg cccgaagtcg gcaccgtcac
      241 cgaaatccgc gggcgcgagg acagcccgac gatttactac tcgttcacgt cggcgctcta
      301 tccgaccacg gtgttcgcct acgacccgcg tacgcgtaag agcacgccgt tcgatccgcc
      361 gccgcgggtt gccgacgcca gccgctatga aagcaggcag ttttttgcga cgtcgaaaga
      421 cggcacgcgc gtgccgttca ccgtaaccgc gcgcaaagat ctgccgcgcg acggcaataa
      481 tccaacgctg ctgctcggct acggcggttt ttcgctcagc cagatgccgt tctaccgccc
      541 gcacgtcgtc gcctggctcg aactcggcgg cgtctgggtc agctcgaaca ttcgtggtgg
      601 tggcgaatat ggcgacgact ggtatcgcgc cgcgcgcgcc gagaaacgcc agaacgggtt
      661 tgacgacttc atcgcggtgg ccgagcacct cgtgcgcgag aaatttaccg cgccctcgcg
      721 gctcgcgatc ctgggcggct ctaacggcgg cctgctggtc gccaccgtgt cgcagcaacg
      781 ccccgatctg tatgccgtcg cgatgcccgc ggttgcggtc accgacatgc tgcgcttcga
      841 caaattcacc ggcgggcgcg cgtggacgct cgaatacggc tcgccgtcgg atccggttca
      901 attcatgcag ttgatcaaat actcgccgct gcataacgtg aagccgggca cctgctatcc
      961 agcgaccttt gtgacgacgg ccgatcacga tgaccgtgtg gtgccgagcc attcttacaa
     1021 gtacgcggct gagatgcagg cgggacaggg ttgcagccgg ccagtcctcg tccgcgtcga
     1081 acccaaggcc tcgcatggct atcgccccac cgaccggctg atcgccgaaa tcgcggacca
     1141 gtggacgttt gcggccgagc acacgggcat gcgagccgct aaaccgtgac catcaagacc
     1201 atcgccatcg tcggcgccgg tcccggcggc ttccgcctcg tcgcgaatct gggcttgatg
     1261 ggttaccggc tccggctcaa cgacattgac gaagccaagc tcaccgcgat ccgcgcgcgc
     1321 ggcggcatcg acgtcgaagg cacacctggc ggatttgcgc cgctcgaact cgcgactacc
     1381 gatctgcgcg cgacagtcga tggcgcggac ctaatcatcg tcgtatccgg cggcaacacg
     1441 cagccgacgg tcgcgcgcgc cttggcacca ttgctcgtcg acggacaaac gatactgctg
     1501 atgcagggca ataccggcgg ctcgctcgtc gttcgccgcg aactcgacgg cgcgggctgc
     1561 aaagcaaaaa tcgatctcgc ggagactgat acctatccgt acgcaatgcg cgcgatcggg
     1621 ccgaccac
//
LOCUS       DATANS010000811         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_671527, whole genome shotgun sequence.
ACCESSION   DATANS010000811 DATANS010000000
VERSION     DATANS010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671527"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VHT22_12055"
     CDS             <1..687
                     /locus_tag="VHT22_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX3138339.1"
                     /translation="NVITVVDAVSAAAGMPIRMAERNIDVCFSGIQKCFMCPPGLALI
                     ATSDRVWDTIRACKHYRHYFNWIKMRDWIELPKARMMGTPPESLIRSLARAVAMMHEE
                     GLPNVYARHALLAEAFQAFVHAAGCELVAREPQYRSHTVSAMLIPKGMKATEVVNRTL
                     ENDNVRLAGGQDTLKETAIRVGHMGPVQPAMLARGVKALARALTGLGMDAKLAQAGVD
                     ACAQVLARGK"
     gene            756..>1684
                     /locus_tag="VHT22_12060"
     CDS             756..>1684
                     /locus_tag="VHT22_12060"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX3138340.1"
                     /translation="MLMRVALSALILSASSYAVAAQAPDVYPSRPIRVIVPSPPGGGN
                     DIMARIGAQRLSEAWSRPVIVDNRPGAGGAIAFETVAHAEPDGYTLLLGSTNLTVLPD
                     ITKVAYDPIKDFAPISLMSTAMNILLVHPSVPADSVKQLIALAKAQPGKLSYASSSVA
                     TSSHLAAEMFCMQAGVKMLHVPYKGAGPALTDLMGGQVQTLFANPAAAIAHVRSGKLR
                     ALAVTGEKRSTIMPEVPTVAEAALPGFEASTWWGILAPSRTSNAIVVKLNDELARALT
                     QPEALNRIAALGADVVGGPPQKLADHLRAEIPK"
BASE COUNT          314 a          531 c          560 g          279 t
ORIGIN      
        1 aacgtgatca ctgtcgtcga tgcggtatcg gcggccgccg gcatgcccat acgcatggcg
       61 gagcgcaaca tcgacgtgtg cttctccggc atccagaaat gtttcatgtg cccgccgggc
      121 ctcgcgctca tcgccaccag cgatcgcgtg tgggatacga tccgcgcgtg caagcactac
      181 cgccattact tcaactggat aaaaatgcgc gactggatcg agctgccgaa ggcgcgcatg
      241 atgggcacgc caccggaatc gctgatccgc agcctcgcgc gcgcggtcgc gatgatgcac
      301 gaagaagggc tgccgaacgt ctacgcgcgc catgcgctgc tcgccgaagc gtttcaagcg
      361 ttcgtgcatg cggccggctg cgagctcgtc gccagggagc cgcaataccg ctcacacacg
      421 gtgtcggcaa tgctcatacc caagggcatg aaagcgacgg aggttgtgaa tcgtacgctg
      481 gagaacgaca acgtgaggct ggcgggcggg caggacacat tgaaagaaac cgcgatccgc
      541 gtcggccata tggggccggt gcagccggcg atgctcgcgc gcggcgtaaa ggcgctcgca
      601 cgcgcgttga ccgggctcgg catggatgcg aagctcgcgc aagccggcgt cgatgcgtgc
      661 gcgcaagtgc tcgcgcgcgg caagtagaaa tcaaattttc cctcctttgc ggagcgagag
      721 agggttgagg cggggcgatg gaaagggggt tggtaatgct tatgcgagtc gcactgagtg
      781 cgcttatcct gtcggcaagc agttatgcgg tcgccgcgca ggcccccgac gtttatccat
      841 cgcgacccat ccgcgtgatc gtaccgtcgc cgccgggcgg cggcaacgac atcatggcgc
      901 gcatcggcgc gcagcggctc agcgaagcat ggtcgcggcc ggtcatcgtc gataatcgcc
      961 ccggcgcagg cggcgcgatc gcgttcgaga cggttgcgca cgcggaaccc gacggttaca
     1021 cgctgctcct gggcagcacc aacctgaccg tgctgcctga catcaccaaa gtcgcgtacg
     1081 acccgatcaa ggattttgcg ccaatctcgc tgatgtcgac ggcgatgaac attctgctcg
     1141 tgcatccgag cgtgcccgcc gattcggtga aacaactgat cgcgctggcg aaagcgcagc
     1201 ccggcaagct cagctacgcg tcatcgagcg tcgcgacgtc ctcgcacctc gccgccgaga
     1261 tgttctgcat gcaggctggc gtcaagatgc tgcatgtgcc ttataagggc gcgggtccgg
     1321 cgctgaccga tctcatgggc ggacaggttc agacgctgtt cgcgaatccg gcggctgcga
     1381 ttgcgcatgt gcgttcgggc aagctgcgcg cgctggcggt caccggcgaa aagcgttcga
     1441 cgatcatgcc cgaagtgccg accgtcgccg aagcggcgct gccgggattc gaggcgagca
     1501 cgtggtgggg catactggcg ccctcgcgca catcgaatgc gatcgtcgtg aaactgaacg
     1561 acgagctcgc gcgcgcgctc acgcagcccg aagcgctcaa tcgcatcgcc gcgctcggcg
     1621 ccgatgtcgt cggcgggccg ccgcaaaaac tcgccgatca cctgcgcgcg gagattccga
     1681 aatg
//
LOCUS       DATANS010000812         3890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_377672, whole genome shotgun sequence.
ACCESSION   DATANS010000812 DATANS010000000
VERSION     DATANS010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377672"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..>3890)
                     /locus_tag="VHT22_12065"
     CDS             complement(188..>3890)
                     /locus_tag="VHT22_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX3138341.1"
                     /translation="TSIGEILNRHDVLRTRFVVIDGEPRQLVAPPEPLRLQIDDLSAL
                     SADMREGEARQLAEAFCRVPFDLERGPLFIARLLRLGADQHWLLLNMHHIVTDHWSSS
                     IFSRELAAVYEAFDRGLPSPLRALPVQYADFAVWQREWLQGPVLERHLDYWRGALADL
                     PALELPFDRPRPAVASARGGRVRFTIDPTLTFALKALALREGATLFMTLLAAFQLLLQ
                     RYSGQDDIAVGVPIAGRSRPELEGLIGFFVNTLVLRGDLSGDPSFVDYLARVRACSLD
                     AYAHQDMPFERLVQELAPIRDLSRNPLFQISFSLQNAPPPRWGLAGLDVQAVEGLFDS
                     SAKFDLYLSVTESAGQLQGRFEYASALFDQATIENMAAQWSTLLAAIAENPGERISDL
                     SLHSPSERARLLAQSNRVAPDCCDASVAELFAVQAARTPGSIAVREGTTTLSYAELDA
                     RANQLARFLGAQGVQRESLVAVALERGIEMTVALLGILKAGAAYLPLDPALPRHWLAS
                     MLEDAGQPLVVTQQRLLDGLPVGAARACCIDRDWAEIARNDRSDPGTRATGGDTAYVM
                     YTSGSTGRPKGVVIPHRAIVRLVTGTDYLQLDSGDVVAHIANPAFDASTFEIWGALLN
                     GAQLASISYQTMLSPAAFTRALDEHAVTAMFVTTALFNQLARVAPEIFRDRDVLFGGE
                     AAEPHRVATALRDGRPRRLVHVYGPTETTTFATWHEVRHVADDAASVPIGRPIANTEV
                     YVLDDKREPVPVGVPGEIYIGGPGLARGYFGRPDLTAERFVSHPFDSAPGALLYRTGD
                     RARLRGDGALEFLGRRDRQVKIRGHRIEIEEVEAALQRLPQVAQAVVLVHGDASDTRQ
                     LTAYVVLAPGVQATPADLWAQLRQTVPDYMAPGAIVMLVAMPMTPNGKIDRAALPAPG
                     DLAQLRTGWHVQPQDPLDHMLAAIWEELLGVRDVGISDNFFDLGGHSLLAAQMIEMIE
                     RTCGCRLPLTTLFAGPTIGDLARAIRNSALEDRAPLVALNATGTRPPFFFLHGDFTGG
                     GFFSHGLARALGDDQPFYAVHPHGLIDPAIPDSIEAMAAERLPAVRAVRPRGPYVLGG
                     HCAGGMVALEIARQLMREGEEVSCVLMIDTVAPRPPKLVFPGVSVGADIQRVRRRSAA
                     ALPAHDPPGSALALYRDVIRKYVPAPYAGRVVVLRPENHPDTRPAMGWTAFVRDAETV
                     VVAGDHHTVITRHLDATAAQVRSCLQIPI"
BASE COUNT          708 a         1297 c         1294 g          591 t
ORIGIN      
        1 gtccggagat cacatgggtc agccagcgct tcccgggtct tccaatgcgc acgctgactc
       61 acgcgccggg cgcaaacgat tgcatcatct atcgcggcga cgatgcgcga ttcatcgccg
      121 acaacctggt tgtcaacggc acccagtaag atgaagttga ggaggcggcg cggaccctca
      181 ggcgccgcta tataggaatc tgcagacagc tacgcacctg cgccgccgtc gcatcgaggt
      241 gccgggtgat caccgtgtgg tggtcaccgg ccacaacaac cgtctccgcg tcccgtacga
      301 acgccgtcca acccatcgcc ggacgcgtgt cgggatggtt ttcgggacgc agcacgacga
      361 cgcgacccgc gtatggcgcc ggaacgtatt tcctgatcac atcgcggtag agcgcgagtg
      421 cgctgcccgg cggatcgtgt gctggcaatg ccgcggcgga acgccggcgg acgcgctgga
      481 tatcggcacc gacactgacg cccggaaaga cgagcttcgg cggacgcggc gcgacagtat
      541 cgatcatcag cacgcaggat acttcctctc cctcgcgcat cagctgtcgc gcgatctcga
      601 gtgcgaccat gcccccggcg caatgacctc ccagtacgta cggaccccgc ggtcgcaccg
      661 cgcggactgc cggcaggcgc tccgcagcca tcgcctcgat cgagtccgga atggccgggt
      721 cgatcaagcc atgcggatgc accgcgtaga acggctgatc gtcgccgagt gcgcgcgcca
      781 gcccgtggct gaaaaaacca ccgccggtaa agtcgccgtg cagaaaaaag aacggcgggc
      841 gcgtgccggt cgcgttgagc gcgaccagcg gtgcgcggtc ttcgagtgcg ctgttccgaa
      901 ttgcccgcgc aagatccccg atcgtcggcc ccgcgaacag cgtggtaagc ggcagcctgc
      961 acccgcacgt gcgctcgatc atctcgatca tttgggccgc gagcagcgag tggccgccca
     1021 gatcgaaaaa attgtcgctg ataccgacgt cgcgcacgcc gagcagctct tcccagatcg
     1081 ccgccagcat atggtccagc gggtcctgag gctgaacgtg ccagccggta cgcagctgcg
     1141 ccaggtcgcc cggcgctggc agcgcggcgc ggtcgatctt gccgttgggc gtcatcggca
     1201 tggcgaccag catcacgatc gctcctgggg ccatatagtc gggcaccgtt tgccggagct
     1261 gcgcccaaag gtcggcgggt gtcgcctgca ctcctggcgc gaggacgacg taggcggtca
     1321 gctgccgcgt gtcggaggca tcgccatgca cgagcacgac ggcctgggca acctgcggca
     1381 gcctttgcag cgccgcttcg acctcctcga tctcgatgcg atgaccgcgg atcttcacct
     1441 gacgatcacg ccggccgaga aattcgagcg cgccgtcgcc gcgcaggcgc gcgcggtcgc
     1501 cggttcgata caggagcgct ccaggagcgg agtcgaacgg atgcgaaaca aaacgttcgg
     1561 cggttagatc gggccgaccg aaataacctc gggccaggcc cgggccgccg atgtagatct
     1621 cgccggggac gccgactggc accggttcgc gcttatcatc gagaacgtac acctctgtgt
     1681 tcgcgatcgg ccgcccgatc ggcacggagg cggcatcgtc cgccacgtgc cgcacctcgt
     1741 gccaagtcgc gaatgtcgtt gtctccgtcg ggccgtaaac gtgtaccagc cggcgcggac
     1801 ggccgtcgcg gagagcggtc gctacgcggt gcggttcggc cgcttcgccg ccgaacaaca
     1861 cgtcgcggtc gcggaagatc tcgggcgcga cacgggcgag ctggttgaaa agtgcggtcg
     1921 tgacgaacat cgccgtcacc gcgtgctcgt cgagcgcgcg ggtgaacgcg gccggggaca
     1981 gcatcgtctg atacgaaatc gaggcgagct gtgcgccgtt gagcagggcg ccccagatct
     2041 cgaatgtcga cgcgtcaaag gcggggttgg cgatgtgcgc aacgacgtct cccgagtcga
     2101 gttgcagata gtcggtgccg gtgaccagcc tgacgattgc acgatgcggg atgacgacgc
     2161 ccttcggccg cccggtcgag cccgacgtgt acatgacgta cgccgtatcg ccaccggtgg
     2221 cgcgggtgcc tgggtccgaa cgatcgttgc gggcgatctc cgcccagtcg cggtcgatgc
     2281 agcatgcgcg cgctgcgcca acgggcaggc catcgagcaa tcgctgttgc gtcaccacca
     2341 gcggctgtcc ggcgtcttcg agcatcgacg ccagccaatg gcgcggcagc gccggatcga
     2401 gcggcaaata ggcagccccc gccttgagta tgccgagcag cgcgaccgtc atctctatgc
     2461 cgcgctccag cgcgaccgcc accagggact cgcgttgcac gccctgtgcg cctagaaagc
     2521 gcgccagctg attcgcgcgc gcgtcgagct ccgcgtagct cagcgtcgtt gtgccctcgc
     2581 gcacggcgat cgagcccggc gtgcgtgcgg cttgcaccgc aaacagctca gcgaccgagg
     2641 cgtcgcagca gtcgggcgcg acacgattcg actgcgccag cagtcgcgcg cgctccgatg
     2701 gactgtgaag cgaaagatcg gagatgcgct cgccgggatt ttcggcaatc gccgctagca
     2761 gcgtggacca ctgcgcggcc atgttctcga tagtcgcctg gtcgaacagc gccgaggcat
     2821 attcgaagcg tccctgcagc tggcccgcgc tttcggtgac ggagaggtag agatcgaact
     2881 tcgcgctgct atcgaagagg ccctcgaccg cctgcacatc gagaccggcg agaccccagc
     2941 gcggcggcgg cgcattttgc agcgagaacg agatctggaa cagtggattg cgggacaggt
     3001 cgcggatggg tgcgagctcc tgcaccagcc gttcgaacgg catatcctga tgcgcgtagg
     3061 cgtcgagaga gcacgcgcgc acccgcgcca gatagtcgac gaacgacgga tcgccgctca
     3121 gatcgccacg caacaccagc gtgttgacga aaaagccgat cagcccctcg agctcgggac
     3181 ggctgcggcc agcgatcggc acgccaactg cgatatcgtc ctgtccgctg taccgctgca
     3241 acagcagctg aaacgcagcc agcaaggtca tgaacaacgt cgccccttcg cgcagtgcca
     3301 gcgccttcag cgcaaacgtc agcgttgggt cgatggtgaa gcgcacgcga ccgccgcgcg
     3361 cgctcgccac ggcaggccgc ggccgatcga acggcaactc cagcgcgggc aaatcggcca
     3421 gtgcgcctcg ccagtaatcg aggtggcgtt ccagcaccgg accttgcagc cattcgcgct
     3481 gccacacagc gaaatcggcg tactgcaccg gcaacgcccg cagtggcgag ggcagcccgc
     3541 gatcgaacgc ctcgtacacg gctgcgagct cgcgggaaaa aatcgaactc gaccagtggt
     3601 cggtgacgat gtggtgcatg ttcaacagca gccagtgctg atcggccccg agccgcagca
     3661 gccgcgcgat gaacagcgga ccgcgctcga ggtcgaacgg cacgcgacag aacgcctcgg
     3721 ccaattgccg ggcttcgcct tcgcgcatgt ccgcggaaag cgccgaaagg tcatcgatct
     3781 ggaggcgcag cggttcgggc ggggcaacca gctgtcgcgg ctcgccgtcg atgaccacga
     3841 agcgcgtgcg cagcacgtcg tggcgattca ggatttcgcc aatactggtt
//
LOCUS       DATANS010000813         1875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_713458, whole genome shotgun sequence.
ACCESSION   DATANS010000813 DATANS010000000
VERSION     DATANS010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713458"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /gene="murB"
                     /locus_tag="VHT22_12070"
     CDS             complement(<1..679)
                     /gene="murB"
                     /locus_tag="VHT22_12070"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX3138342.1"
                     /translation="MADQKNLLRGEPRRNEPMARHTSWRAGGTADRAYAPVDLEDMRL
                     FLAALPRAEPVHVVGLGSNLLVRDGGLRGTVIFTHGALNELRSEGHAIYAQSGVPSPK
                     LARFAALQELVGAEFMAGIPGTVGGALTMNAGCYGGETWGIVESVTTLDRTGQLRQRT
                     PADYELSYRHVKLREANEEWFAAARFRLPRGDGAKSRATIKELLERRIAAQPLNLPNA
                     GSVFRNPP"
     gene            complement(696..>1875)
                     /gene="murC"
                     /locus_tag="VHT22_12075"
     CDS             complement(696..>1875)
                     /gene="murC"
                     /locus_tag="VHT22_12075"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX3138343.1"
                     /translation="DDNPEVVAARAHKIPVVPRAMMLAELMRLKQGIAVAGTHGKTTT
                     TSLVASVLAEADMDPTFVIGGRLEAAGSHAKLGSGEFIVVEADESDASFLYLAPVLAV
                     VTNIDADHMETYGHYFGRLRQAFLDFVQRLPFYGTAVLCVDDPNVREIMPAVTKPVTT
                     YGLSEAAQVRAVDLKPDGGRMHFRVLRTDARGTKRPDLNITLNLAGAHNVLNALAAIA
                     VGMEVGAPDAAIVRALADFKGVGRRFQRYGEIALVKGGQYTLIDDYGHHPTEMAATLA
                     AARGAFPGRRLVLAFQPHRYTRTRDCFEDFARVLCTVDVLLLTEVYAAGEAPIVAADG
                     RALARAVRVQGKVEPIFVEQVADFPAAVANAVNDGDVVLTMGAGSIGSVPAMLSGGTA
                     "
BASE COUNT          324 a          649 c          594 g          308 t
ORIGIN      
        1 tcggcggatt gcggaatacc gaccccgcgt ttggcaggtt gagcggctgt gcggcaattc
       61 gccgctccag gagctctttg atcgtcgcgc gcgatttcgc gccgtcgccg cgcggcaagc
      121 gaaagcgcgc cgcggcgaac cactcctcgt tggcctcgcg caatttgaca tggcggtagc
      181 tgagctcgta atcggcgggc gtgcgctggc gcaactgccc cgtgcggtcg agtgtcgtga
      241 cgctctcgac gatgccccac gtctcgccgc catagcagcc ggcgttcatc gtgagcgcgc
      301 cgccgaccgt gccgggaatg ccggccatga attcggcgcc gacgagctct tgcagggccg
      361 caaaacgcgc gagcttcggg ctcggcacac cgctctgcgc ataaatcgca tggccttcgg
      421 aacggagctc gtttaacgcg ccgtgcgtga aaatgaccgt gccgcgcaag ccgccgtcac
      481 gcaccagcag attgctgccg agcccgacca catgaaccgg ctcggcgcgc ggcaacgccg
      541 cgagaaacaa acgcatgtcc tcgagatcga cgggcgcgta agcgcggtcg gcggtgccgc
      601 cggcgcgcca gctcgtgtgg cgggccatcg gctcgttgcg ccgcggctcg ccccgcaaca
      661 gatttttctg atccgccatg ttcatcgtat tcgcatcacg cagttccgcc gctcaacatc
      721 gcgggcaccg acccaatcga gcccgcgccc atcgtcagca ccacgtcgcc gtcgttgacc
      781 gcgttggcga ccgccgccgg aaaatccgcg acctgctcca cgaaaatggg ctcgaccttg
      841 ccttgcacgc gcacggcgcg cgcgagggcc cggccatcag ccgcaacgat cggcgcctcg
      901 cctgcggcat acacttcggt cagcagcagc acgtcgacgg tgcacagcac gcgcgcgaaa
      961 tcctcgaagc agtcgcgcgt gcgcgtataa cggtgcggct gaaacgcgag caccaggcgg
     1021 cgtccgggaa acgcgccgcg tgcggccgcg agggttgccg ccatttccgt cgggtgatgg
     1081 ccgtaatcgt cgatcagcgt gtactgcccg cctttcacca gggcgatttc gccatagcgc
     1141 tgaaagcgcc ggccgacgcc tttgaaatcc gccagcgcac gcacgatcgc cgcgtccggg
     1201 gcgccgactt ccatgccgac cgcaatagcc gcgagcgcat tgagcacgtt gtgcgcgccg
     1261 gcaagattga gcgtgatgtt gagatcgggc cgcttggtgc cgcgcgcgtc ggtgcgcaaa
     1321 acgcgaaagt gcatgcgccc gccgtcgggc ttcaaatcga cggcgcgcac ctgcgcggcc
     1381 tccgacaaac cgtacgtcgt gaccggcttg gtgaccgccg gcatgatttc gcgcacgttc
     1441 ggatcgtcga cgcacaacac ggcggtgccg taaaacggca ggcgctgcac gaaatcgaga
     1501 aatgcctggc gcaggcggcc gaaataatgg ccgtaagttt ccatgtggtc ggcatcgatg
     1561 ttcgtgacca ccgcgagcac cggcgcgaga tacaaaaatg acgcgtccga ttcgtcggct
     1621 tcgaccacga tgaattcacc gctgcccaat ttcgcgtgcg agccggccgc ctcgagccgg
     1681 ccgccgatca cgaatgtcgg atccatgtcc gcttccgcga gcacgctcgc cacgagactc
     1741 gtggtcgtcg tcttgccatg cgtgccggcc accgcgatgc cctgcttcaa tctcatcaac
     1801 tcggcaagca tcatcgcgcg cggaacgacc ggaattttgt gggcgcgcgc cgccacgact
     1861 tccggattgt cgtcc
//
LOCUS       DATANS010000814         3237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_82326, whole genome shotgun sequence.
ACCESSION   DATANS010000814 DATANS010000000
VERSION     DATANS010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82326"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..982)
                     /locus_tag="VHT22_12080"
     CDS             complement(152..982)
                     /locus_tag="VHT22_12080"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX3138344.1"
                     /translation="MTTMNILRRMSTLVAAFALIAPRAVAADFVFSITEGVTYYQTNR
                     EIQARFQPLSDLLAKALKRPVRMVVVSPYNDLREGLAKQEYDLSFIHPAHVSLAAIKS
                     GQYHSVAWTTGFTDYSVFLLINKDQSYTKFDDLKGQTVVSPDPDSITAAMLRAMLRDQ
                     KFGSTDVKVITTRYQDAVPFYVEYGFAQAGATAAKSVAKSWTSKGGKILLTSRPTPIK
                     QFIASSRLSADDDEKVRAVLTTLGQSDGGKHALTTLGYQGFVAPNRDDELKAIAWLGL
                     "
     gene            1358..2440
                     /locus_tag="VHT22_12085"
     CDS             1358..2440
                     /locus_tag="VHT22_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3138345.1"
                     /translation="MNANPPYLSIEALTVSYGATRVLDHVSLGVDRGEMIALLGSSGC
                     GKTTLLRAIAGFVRPESGAIRVAGKDITGLPPEARGTAMMFQSYALWPHMNVADNIAY
                     GLRMRGWTKAAIAGRVADMLKLLQLDGFGPRPVTQLSGGQRQRVALGRALAIDPHLLL
                     LDEPMSNLDYKVRLELRHELRALQQRIGITAVYVTHDREEALTLSDRIAVIDAGRIVQ
                     VGAPEEIFHRPTSAFVAGFMGADNAIDVAQHDDGALIAASDAGSDRRVRAHFRSDSAR
                     LGTATASTDGSLVLSGTIAQAVYVGQGYRYRVRTAGGDVWVHAPDRLPEGTAACVIVP
                     REAVLLFPRDQDKADKAAAGAMRPAA"
     gene            2584..>3237
                     /locus_tag="VHT22_12090"
     CDS             2584..>3237
                     /locus_tag="VHT22_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3138346.1"
                     /translation="MKSRLIAFASALAFAGAAFAQTTLNVVTAGDQNMVDYVNNYLAP
                     KFEAANPGVKVRAVGTGPGDSGSQKIYEKLSAQQKAGTAEWDVDVAVIHQLASATMVN
                     EKLLMPYRNDISAGKLVSRDTAKNALGANVDGYVMPMFHSQIVFAYNPELVKAPPRSF
                     AELTEWVKKNPKQFGYNGVKGGMSGVGFVTGWVSAYSGIGDKLEKGPFDASSGPAIDK
                     "
BASE COUNT          595 a         1028 c         1048 g          566 t
ORIGIN      
        1 tatcgtccgg gtcgcgaatg cgccccggcc gtgcgtggga gatgaccgga ccgtgtacgg
       61 ccgcctcgcg cgtccaggcg ccattcttag cgtcgggcga tcaatacggc ggcgacgcgg
      121 tacatcggcg gcggcccggg ggcccgccgt ctcacaggcc gagccaggcg atcgccttca
      181 gttcgtcgtc acgattcggt gctacgaatc cctggtaacc gagtgtggta agagcgtgct
      241 tgccaccgtc gctctgcccc aacgtcgtga gcaccgcgcg cactttctcg tcgtcgtcgg
      301 cggacagcct ggacgaggca atgaactgct tgatcggcgt cggccgcgag gtcagcagga
      361 tcttgccacc cttcgaggtc cacgacttcg ccaccgactt ggccgcagtc gcgccggctt
      421 gcgcaaaacc gtactcgacg taaaacggca ccgcgtcctg gtagcgcgtg gtgatcacct
      481 tgacgtcggt cgagccgaac ttctggtcgc gcagcatcgc ccgcaacatt gctgcggtga
      541 tcgaatccgg atcgggactg acgaccgtct ggcccttcag atcatcgaac ttggtgtacg
      601 actgatcctt gttgatcagc agaaataccg agtagtcggt gaagccggtc gtccacgcga
      661 ccgaatgata ctggcccgac ttgatcgcag cgagagaaac gtgcgcggga tggatgaacg
      721 ataagtcgta ttcctgcttg gcaagacctt cgcgcaggtc gttgtagggg ctgactacca
      781 ccatgcgcac cggccgcttc agcgctttcg ccagcaggtc cgacaacggc tggaaccgcg
      841 cctggatttc gcggttggtc tgatagtagg tgacgccctc ggtgatcgaa aaaacgaaat
      901 ccgcggccac tgcgcgcggt gcaatgagcg cgaacgccgc caccaaggtc gacatcctgc
      961 gcaaaatgtt catcgtcgtc atgcgtgatt cccctgatca ttccgggccg cgtgcgcctg
     1021 ttggcggctt gtcgccgcgg ccccgaaaaa gcatacgccc aaccgccaaa cacccgttgt
     1081 catgacatcg tcattaccgg gtcgcgcgaa cgcgacacgg cgcggcgctt tgcgacgcag
     1141 cgcgtcaccc gatcggcacc atcacgccga gcgatcagtc gctcaacggg cggaaggagc
     1201 acgatcgtcg caatcatcca agcctatgca actctcgttc cagctgaact gaacaccttg
     1261 aacgccatct gcaattacgc cacaatcgcc gatgcaccga ccgccatatt cgagcgcatt
     1321 tcctgctgcg tcggacgctg cgtgacgcag tgacacattg aacgcaaacc caccgtatct
     1381 ttcgatcgaa gcgctgaccg tctcgtacgg cgcgacgcga gtactcgacc acgtcagcct
     1441 gggagtcgac cgcggcgaga tgatcgcgct cctgggcagc tccggctgcg gaaagaccac
     1501 gttgttgcgc gcgatcgccg gattcgtgcg tccggaatcg ggcgcgatcc gggtcgccgg
     1561 caaggacatc accggcctgc cgcccgaagc gcgcgggacg gcgatgatgt tccagtccta
     1621 tgccctgtgg ccgcacatga acgtcgccga caatattgcc tacggtttgc gcatgcgcgg
     1681 ctggacgaaa gccgcgattg ccggccgggt cgcggacatg ctgaagctgt tgcaactcga
     1741 cggcttcggg ccgaggccgg tgacgcaact gtccggcggc cagcgtcagc gcgtcgctct
     1801 cgggcgcgca ctggcgatcg acccgcacct cttgctgctc gacgagccga tgtcgaacct
     1861 cgactacaag gtgcgcctgg agctgcgcca tgagctgcgc gcgctgcagc agcgcatcgg
     1921 cataacggcg gtctatgtca cgcatgatcg ggaggaagcg ctgaccttgt ctgaccggat
     1981 cgcggtcatc gacgccggcc gcatcgtcca ggttggtgcg cccgaggaaa tctttcatcg
     2041 accgacgtcg gcgttcgtgg ccggcttcat gggagcggac aacgcgatcg atgtcgctca
     2101 gcacgacgac ggcgcgctga tcgcggcgtc ggacgccgga tcggaccggc gcgtgcgcgc
     2161 gcatttccgc agcgattcgg ctcgactggg caccgcgacg gcgtcgacgg atggcagcct
     2221 ggttctctcg gggacgattg cgcaggccgt gtatgtcggc cagggctatc gttaccgcgt
     2281 gcgcaccgcc ggcggcgatg tgtgggtcca tgcgccggac cgcctgcccg aagggaccgc
     2341 cgcctgcgtc atcgttccgc gcgaagcggt gctgcttttt ccgcgcgacc aggacaaggc
     2401 ggacaaggcc gccgcgggcg caatgcgtcc ggcggcatag ccgggcgccg atgtcggttg
     2461 cggacgattt ggggtattgt cgtcaattgt gactgcactg ccgcggcggt gcgggcggat
     2521 gcgccgtttg cgcgagcgac atctgccacg agaatcgccg ccggaacaaa ataggagggt
     2581 tcgatgaaat ctcgcctgat tgcattcgct tccgcgctcg ccttcgccgg cgcggcgttc
     2641 gcgcaaacca cgctgaacgt cgtcaccgcg ggcgaccaga acatggtcga ctacgtcaac
     2701 aactacctgg cgccgaaatt cgaggcggcg aaccccggcg tcaaagtgcg cgccgtgggc
     2761 accggccccg gtgattcggg ttcgcagaag atctatgaaa agctgtcggc acagcagaag
     2821 gcgggtaccg ccgaatggga tgtcgacgtg gcggtgatcc accagctcgc gtcggcgacg
     2881 atggtcaacg agaagctgtt gatgccgtat cgcaacgaca tcagtgccgg caagctggtc
     2941 tcgcgagaca cggccaagaa cgcgctgggc gcgaacgtcg acgggtatgt gatgccaatg
     3001 ttccacagcc agatcgtgtt cgcctacaac cccgagctcg tgaaggcgcc gccgagatcg
     3061 ttcgccgaat tgaccgagtg ggtgaaaaag aacccgaaac aattcggcta caacggcgtc
     3121 aagggcggga tgtcgggcgt gggcttcgtc accggctggg tgtcggcata cagcggcatc
     3181 ggcgacaagc tggaaaaggg cccattcgat gcctcttcgg gaccggcgat cgacaag
//
LOCUS       DATANS010000815         1898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_41286, whole genome shotgun sequence.
ACCESSION   DATANS010000815 DATANS010000000
VERSION     DATANS010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41286"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VHT22_12095"
     CDS             <1..318
                     /locus_tag="VHT22_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEX3138347.1"
                     /translation="GVLCFFAATAVKRMFGYDDSLDAFGVHCIGGIVGALLTGVFNVK
                     DISGTDGDFMVQLGAVATTLVYSGVGSFIILKVIDMVVGLRVSEEEEREGLDISLHGE
                     RVE"
     gene            complement(397..1326)
                     /locus_tag="VHT22_12100"
     CDS             complement(397..1326)
                     /locus_tag="VHT22_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX3138348.1"
                     /translation="MRASSRASPPPRDARAEPLVLAFERYLSTRPAHTRSAYRRDVRI
                     LQQLAGNLPLAKLSPAELRRFLALLHARGLSGKSLARVLSGWRAFYRFAIDHEPSIKD
                     NPCAGLKAPRSSRHLPAVLSPDEAVRLVAIADEDVLAVRDRALLELAYSSGLRVSELA
                     GLELAALDLAEGEVRVLGKGAKERIVPVGEPARVSLKAWLALRPTLPAPDSEAVFVSR
                     SGRRLTSRAIQQRLAGWAVKRGLARHVHPHMLRHSFASHLLQSSGDLRAVQELLGHAS
                     IASTQIYTHLDFQYLAKIYDQAHPRAKKQRARR"
     gene            complement(1332..>1898)
                     /locus_tag="VHT22_12105"
     CDS             complement(1332..>1898)
                     /locus_tag="VHT22_12105"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEX3138349.1"
                     /translation="PHPHGGHAIPIAERQIVTLREKNGQLESKLRELIQFGTDNDAIS
                     EKLHRSTLALFSAADLETTLGVLYHSLSEDFRVPQVTLRLWGRVPEQSYLPEFAATSQ
                     EIREYASALGAPYCGTQAPFESREWFEGGASLASFALLPLRTHHTFGLLALGSEDPGR
                     FYAGLGTMHLTRLSELASVATARFLPQG"
BASE COUNT          326 a          609 c          648 g          315 t
ORIGIN      
        1 ggcgtgctgt gctttttcgc cgcgacggcc gtcaagcgga tgttcggcta cgacgactcc
       61 ctcgatgcct tcggcgtgca ctgcatcggc ggtatcgtcg gtgccctgct gaccggcgtg
      121 ttcaacgtca aggacatcag cggcaccgat ggggacttca tggtgcagct gggcgcggtc
      181 gcaacgacgc tggtctacag cggcgtcggc agcttcatca tcctcaaggt catcgacatg
      241 gtcgtcggct tgcgcgtctc cgaagaagag gagcgcgaag gcctcgacat cagtcttcac
      301 ggcgagcgcg tggagtaggc cgggtactac gtccggcctg gagtttctcc tccgtgtatt
      361 tcggggcgcc gcaagcgccc cgtttttttt gaggcgtcag cgccgcgcgc gctgcttctt
      421 cgcccgcgga tgggcctggt cgtagatttt ggccaggtac tgaaagtcga ggtgcgtgta
      481 gatctgcgtg ctggcgatgc tcgcgtgacc cagcagctcc tgcacggcgc gcagatcgcc
      541 ggaggactgc agcaggtgcg aggcgaagga atgccgcagc atgtgcggat ggacgtgccg
      601 ggcgagtccc cgcttgaccg cccatccggc gaggcgttgc tggattgccc gcgatgtgag
      661 tcgtcttccc gagcgactaa cgaacacggc ttcactgtcg ggcgccggca aggtcggccg
      721 cagcgcgagc cacgccttga gcgaaacgcg ggcaggctct ccgacgggaa cgatgcgctc
      781 cttggcgcct ttgcccagca cgcgcacctc gccttcagcc agatcgagcg ccgcaagctc
      841 gaggccagca agctcggaca cgcgcaggcc cgacgaatac gctagctcga gcagcgcgcg
      901 gtcgcgcacc gcgagcacgt cttcgtcggc gatggcgacc agtcgcaccg cctcgtcggg
      961 cgagagcacc gccggcaaat ggcgcgacga ccgcggagcc ttgagccccg cgcacgggtt
     1021 gtccttgatc gacggctcgt gatcgatggc gaaacgataa aaagcgcgcc agccggacag
     1081 gacgcgcgcg aggctcttgc ccgacaagcc gcgcgcatgc agcagcgcca gaaagcgtct
     1141 gagctcggcg ggagatagct tggcgagcgg caagtttccg gcgagctgct gcaatatccg
     1201 gacgtcgcgg cggtaggcgc ttcgggtgtg cgcgggccga gtcgacagat accgctcgaa
     1261 cgccaggacc agtggttcgg cccgcgcatc ccgcggcggc ggactggcgc gggacgaagc
     1321 gcgcatcagg atcagccttg cggcagaaaa cgtgcggtcg cgacgctggc gagctccgac
     1381 aagcgagtca gatgcatcgt tccgaggccc gcatagaagc ggcccggatc ctcactgccg
     1441 agcgccagca gaccgaacgt atggtgcgtg cgcagcggca ggagcgcgaa cgatgcaagt
     1501 gacgcgccgc cttcgaacca ttcacgcgac tcgaacggcg cctgcgtgcc gcagtagggc
     1561 gcacccaggg cgctggcgta ttcgcgaatt tcctggctgg tcgccgcgaa ctccggcaga
     1621 tacgactgct cgggcaccct gccccacagg cgcagcgtca cctgcggcac acgaaaatcc
     1681 tcgctcaggc tgtggtacag aacacccagc gtggtttcca ggtccgctgc ggaaaagagc
     1741 gcgagcgtcg agcgatgcaa cttttcgctg attgcatcgt tatcggtgcc gaactggatc
     1801 agctcgcgca gcttcgattc gagctggccg ttcttctccc gcagggtcac gatctgccgt
     1861 tcggcgatgg gaatcgcatg accgccgtgc ggatgcgg
//
LOCUS       DATANS010000816         3786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_377853, whole genome shotgun sequence.
ACCESSION   DATANS010000816 DATANS010000000
VERSION     DATANS010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377853"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..423
                     /locus_tag="VHT22_12110"
     CDS             1..423
                     /locus_tag="VHT22_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HEX3138350.1"
                     /translation="MAGRRYNAPMDACSRPDSWRAAMLAIVAIVACLTSPLATPKTSS
                     APAEIRIDALPAEAREALERIRTGSRLPYERDGVVFGNRERLLPERAHGYYHEYTVKT
                     PGMHSRGGRRIVCGGAIASTSECYYSDDHYRSFKRIRQ"
     gene            420..809
                     /locus_tag="VHT22_12115"
     CDS             420..809
                     /locus_tag="VHT22_12115"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEX3138351.1"
                     /translation="MKVPDLSTAADAGVHDWTGAPEPLKTAAAAARLRFCSLDLHGID
                     NKTALLGTLGKSLKLPEHFGDNWDALADCLEDSDWLGAHGIAIVLRHAAAYRKAHAAD
                     WETLSDILSEAAEYWQERHKPFWVFVS"
     gene            854..1318
                     /gene="nudB"
                     /locus_tag="VHT22_12120"
     CDS             854..1318
                     /gene="nudB"
                     /locus_tag="VHT22_12120"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEX3138352.1"
                     /translation="MPHKLPLSTLVLVHTPDLKVLLLERADFPGYWQSVTGSQEAGET
                     LAETARRELHEETGIDADRYGGVSDWNLSNVYEIYPQWRFRYPEGTTHNTEHVFSVEV
                     PQPVPVILNAREHLAHDWLSWTEAAPKCFSWSNRAAIELLPQRVASSPRRTR"
     gene            1315..2049
                     /locus_tag="VHT22_12125"
     CDS             1315..2049
                     /locus_tag="VHT22_12125"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX3138353.1"
                     /translation="MISVQIARAAAMAALLAVSATAGLASAQVPGVPTQPVVTVSASA
                     TVTVSNDRLQAWLRAEAENPSPAAAASQVNAIIAKALAGARSYPAVKLATAGYSTQQI
                     SEKGKPSRWRVAQAVSLDSADFTAAAILITKLQDEDGLLLSGMGFSLSDKTRRDAEDG
                     VTRQAIRSWQSRAEQAAQALGFASWRPGHLTVQAGDGGRVYPTMRAQATPGLAQAPIA
                     LEAGTTDVTVTVNGEALLEPAITPAR"
     gene            complement(2065..3462)
                     /gene="fumC"
                     /locus_tag="VHT22_12130"
     CDS             complement(2065..3462)
                     /gene="fumC"
                     /locus_tag="VHT22_12130"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX3138354.1"
                     /translation="MSQSTTRIERDTFGPIEVPADRLWGAQTQRSLEHFRISTEKMPA
                     SLIRAMLIVKRSAARVNLALGILSPKVADAIVAACDEALVGAHAAEFPLAVWQTGSGT
                     QTNMNVNEVLANRASELLGGERGEARLVHPNDDVNRGQSSNDVFPTAMNVASAETVAR
                     DLLPALDALQATLAAKAESFAAIVKIGRTHLQDATPLTLGQEFSGYAAQLAHARMHVD
                     AALPHVHELALGGTAVGTGLNTHPEFGARAAADIAEFTGLPFVSAPNKFEAMAAADAL
                     VNLHGALKTLAAVLTKIANDVRWLASGPRSGLGEITIPENEPGSSIMPGKVNPTQCEA
                     LTMLCAQVLGNDVAVNIGGASGNFELNVYRPLIIHNVLQSIRLLGDGMASFDAHCATG
                     IEANLARIDELLERSLMLVTALNPHIGYDKAAAIAKKAHREGTTLKAAALALGYVSAE
                     QFDQWVQPQRMIGPQ"
     gene            complement(3532..>3786)
                     /locus_tag="VHT22_12135"
     CDS             complement(3532..>3786)
                     /locus_tag="VHT22_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX3138355.1"
                     /translation="RLLDFGAIVQVLPGKDGLLHISQIAKERVNQVSDYVKEGQIVKV
                     KVLEADDKGRLRLSMKAAAEDAAAAAPAAQTTPSEQTPAV"
BASE COUNT          637 a         1257 c         1222 g          670 t
ORIGIN      
        1 attgccggcc ggcgttacaa tgcgccgatg gatgcctgct cacgcccgga ttcatggcgg
       61 gcagcgatgc tggcgatcgt cgcgatcgtc gcctgcctca cctcgccgct tgctacaccg
      121 aaaacttcct ctgcgccagc cgagatccgc atcgacgcgc tgcctgcgga agcgcgcgag
      181 gcgctcgagc gcatccgcac gggtagtcgc ttgccctacg agcgcgacgg cgtcgtgttc
      241 ggcaatcgcg aacgcctgct gcccgagcga gcgcacgggt actatcatga gtacactgtt
      301 aaaacgccgg gcatgcatag ccgcggcggg cgtcggatcg tctgcggcgg cgcaatcgca
      361 tcgacgtcgg agtgctacta ttccgacgac cactatcgtt cgttcaagag aatacggcaa
      421 tgaaggttcc cgacctttcc acagctgcgg acgcaggcgt gcatgactgg accggcgcgc
      481 ccgaaccgct gaagaccgca gccgccgctg caaggctcag gttttgctcg ctcgacctgc
      541 acggcatcga caacaagacc gcgttgctgg gaacactggg caagagtctc aagctgcccg
      601 agcatttcgg cgacaattgg gatgcgctgg ccgattgcct cgaagacagc gactggctcg
      661 gcgctcacgg catcgcgatc gtgctgcggc acgccgcagc ctatcgcaag gcgcacgccg
      721 ccgactggga aaccctttcc gatatcctga gcgaggccgc cgaatactgg caggagcggc
      781 acaagccctt ctgggtattc gtcagctagc cgccgattcg acagcggcgg cacattcgat
      841 tgggtcgctt tcgttgccgc acaagcttcc cttatcgacg ctggttctgg tgcacacgcc
      901 ggatctcaag gtcctgctgc tggagcgcgc cgactttccg ggctattggc agtccgtgac
      961 cggcagccag gaagcgggcg agacgctcgc cgaaaccgcg cggcgcgagt tgcacgagga
     1021 gaccggaatc gatgccgacc gctacggcgg cgtctctgac tggaacctct ccaatgttta
     1081 cgagatctat ccacagtggc gctttcgcta tcccgaggga acgacgcaca ataccgagca
     1141 cgtattctcg gtagaggtgc cccagccggt gccggtgatc ctgaatgcgc gtgaacacct
     1201 ggcgcacgac tggctgtcct ggacggaagc cgcacccaag tgcttttctt ggtcgaatcg
     1261 ggcggcgatc gagttgctgc cgcagcgagt tgccagcagt ccaaggagaa ctcgatgatc
     1321 tctgtgcaga ttgcgcgcgc ggcggcgatg gcagcgctgc tcgctgtcag tgcgacggca
     1381 ggcttggcca gcgcgcaggt gcccggcgtg ccgacgcaac ctgttgtcac cgtctcggcc
     1441 tccgccacgg taacggtaag caacgatcgc ctgcaggcat ggttgcgcgc cgaggccgaa
     1501 aatcccagcc cggccgccgc tgcgagccag gtcaacgcga tcatcgccaa ggcgctcgca
     1561 ggcgcgcgat cgtatccggc cgtcaaactc gcgacggcgg gctactcgac gcagcaaatc
     1621 agcgaaaagg gcaagccgag ccgctggcgc gtcgcgcaag cggtaagcct ggactcggcc
     1681 gatttcaccg cagcggcgat cctgatcacc aagcttcagg acgaggacgg actattgttg
     1741 tcgggcatgg gattctcgct gtccgacaaa acacggcgcg atgcggaaga cggcgtgacg
     1801 cggcaggcga tacgcagctg gcaatcgcgc gccgagcagg cggctcaggc tttgggcttc
     1861 gccagctggc gtcccgggca tctgaccgtg caggccggcg acggcggcag ggtctatccg
     1921 acgatgcgtg cgcaggccac gccggggctt gcgcaggctc cgatcgcgct cgaggctgga
     1981 acgaccgacg tgaccgtcac cgtgaacggc gaggccctcc tcgagcccgc gatcacgccg
     2041 gcgcgctgac cggtccgcgc gtatctattg tggcccgatc atccgctgcg gctgcaccca
     2101 ctggtcgaac tgctccgcgc tgacgtagcc cagcgcgagc gccgcggcct tcagcgtcgt
     2161 tccctcccga tgcgccttct tcgcgatcgc cgccgccttg tcgtagccga tatgcggatt
     2221 gagcgcggtg accagcatca gcgagcgctc gagcaattcg tcgatgcgcg cgagattggc
     2281 ctcgatgcca gtggcgcaat gggcgtcgaa gctcgccatc ccatcgccga gcaggcggat
     2341 actctgcagc acgttatgga tgatcagcgg ccggtagacg ttcagctcga agttgcccga
     2401 cgctccgccg atgttcaccg cgacgtcgtt acccagcacc tgcgcgcaga gcatcgtcag
     2461 ggcctcgcat tgcgtcggat tgaccttgcc tggcatgatc gagctgcccg gctcgttttc
     2521 cggaatggtg atttcgccca gtcctgagcg cgggccgctg gccagccatc gcacgtcgtt
     2581 cgcaatcttg gtcagcacgg cggccaaagt cttcagcgcg ccgtgcaggt tgaccagcgc
     2641 atcggcggcc gccatcgcct cgaatttgtt tggcgcgctg acaaatggca acccggtgaa
     2701 ctcggcgata tcggccgcgg cgcgagcgcc gaattccggg tgcgtgttta gcccggttcc
     2761 gaccgccgtt cctcccagcg ctagctcgtg gacgtggggc aacgctgcgt cgacgtgcat
     2821 tcgcgcatgc gcgagctgcg ccgcatagcc ggagaattcc tggcccaggg tcagcggcgt
     2881 cgcatcctgc agatgggtgc ggccgatctt gacgatagcg gcaaagcttt ccgcctttgc
     2941 cgcgagcgtc gcctgcagcg cgtcgagggc gggcagcaga tcgcgcgcca ccgtctcggc
     3001 cgaggcgacg ttcatcgccg tcggaaatac gtcattcgac gattggccgc gattgacgtc
     3061 gtcgttcgga tgcacgagcc tcgcctcgcc gcgctctcct cccaggagct ccgaggcgcg
     3121 attggccagc acctcgttga cgttcatatt ggtctgcgtg ccggatccgg tctgccagac
     3181 ggccagcggg aattcggccg cgtgcgcccc gaccagcgcc tcgtcgcagg ccgcgacaat
     3241 cgcgtcggcg accttaggcg aaaggatgcc cagagccaga ttgacgcgag cggcgcttcg
     3301 cttgacgatc agcatcgcgc gtatcagcga cgccggcatc ttctcggtcg agatgcggaa
     3361 gtgctcgagc gaccgctgcg tctgcgcgcc ccacagccgg tcggcgggga cttcgatcgg
     3421 tccgaaggtg tctcgctcga tccgtgtcgt ggactggctc atcaatggtc tccaggcgga
     3481 aaaaaagggg cgccgcgcgc cccctttttc attgctccgc catatgggtt actacaccgc
     3541 cggcgtttgc tccgacgggg tcgtctgcgc cgcgggggcg gcagctgcgg catcttcggc
     3601 cgcggccttc atcgacaggc gcagccgccc cttgtcgtcc gcctcaagca ccttgacctt
     3661 gacgatctgg ccttccttga cgtagtcgga gacctgattc acgcgctcct tggcgatctg
     3721 cgagatatgc agcagaccgt ccttgccagg cagtacctgc acgatcgcgc cgaaatcgag
     3781 caggcg
//
LOCUS       DATANS010000817         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_587427, whole genome shotgun sequence.
ACCESSION   DATANS010000817 DATANS010000000
VERSION     DATANS010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587427"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..2022)
                     /locus_tag="VHT22_12140"
     CDS             complement(1..2022)
                     /locus_tag="VHT22_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEX3138356.1"
                     /translation="MFSKILIANRGEIACRVARTARRMAVLTVAVYSDADAGALHVET
                     CDEAYRLGPPPPRESYLAGDKIIAIARRAGAQAIHPGYGFLSENAEFATAVAAAGLVF
                     IGPPPAAIRAMGSKAESKTIMGRAGVALVPGYHGADQDDALLRREADAIGYPVLIKAS
                     AGGGGKGMRVVERSEDFIAALGSARREAKASFGDQTVLLEKYLLEPRHIEMQVFADTH
                     GECIHLFERDCSVQRRHQKVVEEAPAPGMSAARRAKMGAAAVAAAKAIGYVGAGTVEF
                     IVETAGAGPFYFMEMNTRLQVEHPVTEMITGLDLVEWQLRVAAGERLPRRQEEVAITG
                     HAIEARIYAEDPDRGFLPSTGRIAHLSSPPRSEHVRIDSGVRNGDEISRFYDPMLAKL
                     VASGADRPEALRRLSDALAQYRVVGVTTNVAFLQRLIAHPAFAEARLDTGLIARHHAD
                     LFPPATAPSATTLAVAALGEISRLRETAATAAALSPDPYAPWHAIEGWWLNSDDHPIR
                     LTYAFGDDEYGVDVRVAHGAGGWVVATLGKSMSASVSGAGESMEVVIDGVHMAVQVVP
                     LGDERHVFCGGEEHRLRVVDPLAHAGEEPRQGGHLTAPMSGAIVAVLVKAGDTVARGA
                     PLLILEAMKMEHTIVAPTAGTVSAIHYRQGDQVPEGADLIDVEAEAEKT"
     gene            complement(2046..>2254)
                     /locus_tag="VHT22_12145"
     CDS             complement(2046..>2254)
                     /locus_tag="VHT22_12145"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX3138357.1"
                     /translation="NWAASFGEDMLVGVLIYLALAHPIGFFIVLALLIALTIWMLPKL
                     VRSLRAMIERIQRWFGTAAGRPDA"
BASE COUNT          374 a          788 c          728 g          364 t
ORIGIN      
        1 tcaagtcttt tccgcctcgg cttcgacgtc gatcaggtcc gccccctcgg gcacctggtc
       61 tccctggcga taatgaatcg cactgacggt gcctgcggtc ggcgcgacga tcgtatgctc
      121 catcttcatc gcttcgagaa tcagcagcgg cgcgccgcgg gccacggtgt cgcccgcctt
      181 gaccaggacc gcgacaatcg cacccgacat cggcgcagtc aggtgacctc cctgccgggg
      241 ctcctcgccg gcgtgcgcca gtggatcgac cacgcgcagc cgatgctctt cgccgccgca
      301 gaacacatgg cgctcgtcgc caagcggcac tacctgaaca gccatgtgca cgccgtcgat
      361 gacaacttcc atcgactcgc cggcgcctga gaccgacgcg gacatcgact tgccgagcgt
      421 cgcgacgacc cagccgccag caccatgcgc gacgcgcacg tcgactccat attcgtcatc
      481 gccaaaggcg taggtcagcc tgatcgggtg gtcgtcgctg ttgagccacc agccttcgat
      541 cgcgtgccat ggcgcatagg gatcgggcga caacgccgcg gcggtggcgg ccgtctcgcg
      601 cagccgcgaa atctcgccca gcgctgcgac ggccaatgtc gtcgccgacg gcgcggtcgc
      661 gggcgggaac agatcggcgt ggtgccgcgc gatcaatccg gtatcgagtc gtgcctcagc
      721 aaacgccgga tgcgcgatca ggcgctgcaa aaaagcgaca ttcgtcgtga cgccgacgac
      781 gcggtactgc gcaagcgcgt cgctgagacg gcgcagcgcc tcgggtcgat cggcgccgga
      841 agcgacgagc ttggcaagca tcggatcgta gaagcggctg atctcgtcgc cgttgcgcac
      901 gccgctgtcg atgcgcacgt gttccgagcg cggtggcgag ctcaggtggg cgattcgacc
      961 agtcgacggc aggaaaccgc gatccggatc ctcggcgtag atccgggcct cgatcgcgtg
     1021 gccggtgatc gcgacctctt cctgccggcg cggcaggcgc tcgcccgccg cgacccgcaa
     1081 ttgccactcg accaggtcga ggccggtgat catctcggtg accggatgct cgacctgcag
     1141 gcgggtgttc atttccatga aatagaatgg gccggcgccc gccgtctcga cgatgaactc
     1201 gacggtgccg gcgccgacgt aaccgatcgc ctttgccgcc gcaaccgccg cggcgcccat
     1261 tttggcgcgc cgcgcagcgc tcatccccgg cgccggtgcc tcctcgacga ctttctgatg
     1321 gcgccgctgc accgagcaat cgcgctcgaa caagtggatg cattcgccgt gcgtgtcggc
     1381 gaatacctgc atttcgatat gccgcggttc gagcaggtat ttttcgagga gcaccgtttg
     1441 gtcaccgaag ctcgctttgg cctcgcgccg cgccgagccg agcgccgcga tgaaatcctc
     1501 cgatcgctcg acgacgcgca tgcctttgcc gccgccgccg gccgacgcct tgatcagcac
     1561 cgggtagccg atcgcatcgg cctctcgccg cagcagcgca tcgtcctggt cggccccgtg
     1621 atagccggga accagcgcaa ccccggcgcg tcccatgatc gtcttcgact ccgccttcga
     1681 gcccatcgcc cggatcgccg ccggcggcgg accgatgaaa acgagccccg ccgcggcgac
     1741 ggcggtggcg aactccgcgt tctcggagag aaatccgtag cccggatgaa ttgcctgcgc
     1801 gccggcccgt cgggcaatcg cgatgatctt gtcgccggcg aggtaacttt cccgcggcgg
     1861 cggcggtccg agacgatacg cctcgtcgca ggtctcgacg tgcagcgcgc cggcatcggc
     1921 gtcggagtag acggcaacgg tgagcacagc catgcgccgc gcggtgcgcg ccacccggca
     1981 ggcgatctcg ccgcggttgg cgatgagtat cttgctgaac atcgggtgag cggccatcgc
     2041 gctgttcagg cgtcgggccg gcccgcggcc gtgccgaacc aacgctgaat cctttcgatc
     2101 atcgcccgga gcgaacgcac cagtttcggc agcatccaga tcgtcaaggc gatcagcagt
     2161 gccagcacga tgaaaaatcc gatcggatgc gcgagagcca ggtagatcaa tacgccgacc
     2221 agcatgtctt cgccaaacga ggcggcccag ttcg
//
LOCUS       DATANS010000818         3287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_713784, whole genome shotgun sequence.
ACCESSION   DATANS010000818 DATANS010000000
VERSION     DATANS010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713784"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /gene="pepN"
                     /locus_tag="VHT22_12150"
     CDS             complement(<1..779)
                     /gene="pepN"
                     /locus_tag="VHT22_12150"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEX3138358.1"
                     /translation="MLPRDAAPPVVQRLAYRPPSHLVDDIELRFELDPAATRVAARFA
                     YRRNPAADPGAALVLGGEQLKLIEVSLDGRPLSQGEYALSSAGITFRTLPDTGVLHIV
                     SECDPAANRALEGLYLSSGVFCTQCEPEGFRRLAFFQDRPDVLARYTVTIVADSQRYP
                     VLLSNGNLIERGAVPGDAGRHYAIWHDPYPKPSYLFALVAGDLASLDDEFITASGRKV
                     ALRIFSTPVNLAKCHHAMAALQRAMRWDEERYGREYDLDTF"
     gene            complement(779..1237)
                     /locus_tag="VHT22_12155"
     CDS             complement(779..1237)
                     /locus_tag="VHT22_12155"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX3138359.1"
                     /translation="MRLLLLAAALLLTTCLALAQTGTTDSSTTSSKMDAKLNELQKID
                     NKVGTGAEAIKGASVVVHYTGWLYAPVAPDHKGTKFDSSRDRNEPFVFPLGGGRVIRG
                     WDEGVAGMKVGGQRTLIIPPDMGYGSRGAGGVIPPGATLVFDVELLDVKR"
     gene            1517..3226
                     /locus_tag="VHT22_12160"
     CDS             1517..3226
                     /locus_tag="VHT22_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138360.1"
                     /translation="MKAMFGFLTAQKDSGDPLQSAKAAAAWLRQLPALDVIGRQQHVI
                     QALDAVRGTQRPIDLNRITAIEFVDAALGADRRQLIKQYIENIETSPKLAERIWQALW
                     EMSQAFTLAYQQALESALPQAGNARWRAALPLLFVRFVHFHGTDAKLRVFKYERWIPA
                     KWIELHQAYLRACEMQCDRQAVVLPAAGQGAQQWTVEQEYLYVLLVHQLNTGNLNPTE
                     IDWASSQLRAWTRRLELEQIPRSLEGFFVDLAGREGLIRRTGNDRGSVLRYLDTTPLA
                     DGLERAITALRDAELTDQGPVAAINQQRLSVLRKIQPAVSPTSETELRRDPRVAVAVS
                     ARVRIGLSRICQDLGAQAAGENGGEVGTGTEQIEVFAVAGAPRIRRKASEDDSLAVSL
                     SSWTDPTWEIKDRSVAGLRIAATGGIGQSLTLGTLVAVRQSDAGGWLLGVVRRLNKVS
                     NEEVEAGVNIIAERVVAVTLAAKRRAHEDMGYVINGLDMSTMGERFEGLYLPPPSRPD
                     KPLAMKTIIVPTSEYAEGRNVLLTTARSVYTVSMRHLVEQRPDWSWATIQLVDKKVRE
                     EAA"
BASE COUNT          632 a         1019 c         1081 g          555 t
ORIGIN      
        1 atgaaggtgt cgaggtcgta ttcccgtccg tagcgttcct cgtcccagcg catcgcacgc
       61 tgcagcgccg ccatcgcgtg atggcatttg gcgagattga ccggcgtcga gaaaatacgc
      121 agtgcgactt tgcgtcccga cgcggtgatg aactcgtcgt cgagggacgc caggtcgccg
      181 gcgaccagtg cgaaaaggta cgagggcttc ggatacgggt cgtgccagat cgcgtagtgc
      241 ctgcccgcgt cacccgggac agcgcctcgc tcgatcagat tgccgttcga aagcagcacc
      301 gggtagcgct ggctgtcggc gacgatcgtc accgtatacc gcgccagcac gtccggccgg
      361 tcttggaaaa aagcaaggcg gcgaaaccct tccggttcgc actgtgtgca aaacacgccc
      421 gatgacaggt ataagccctc gagcgcgcga ttggcggcag ggtcgcattc ggagacgatg
      481 tgcagtacac cggtgtcggg gagtgtccga aaagtgattc cggccgacga aagtgcgtat
      541 tcgccctggg acagcggccg cccgtcgagg ctgacctcga tcagcttcag ctgctcgccg
      601 ccgagcacca gcgcggcgcc agggtcggcc gccggatttc tgcggtaggc gaatcgcgcc
      661 gctacccgcg tggccgcagg atcgagctcg aagcggagct cgatgtcgtc gacgaggtgc
      721 gaaggcgggc gataggcgag ccgttgtacg accggcggcg ccgcgtcgcg aggcagcatc
      781 agcgcttgac gtccagcaac tcgacgtcga agaccagcgt cgcacccggt gggatgacgc
      841 cgcctgcgcc gcggctgccg tatcccatgt ccggagggat gatcagcgtg cgctgcccgc
      901 cgactttcat gccggccacg ccttcatccc agccgcgaat cactcgccct ccgccgagcg
      961 ggaacacgaa gggctcattg cgatcgcgcg acgagtcgaa cttggtcccc ttgtggtcgg
     1021 gtgcaacggg cgcatacagc cacccagtgt agtgaacaac taccgacgca cctttgattg
     1081 cttctgcgcc ggtgccaacc ttgttgtcga tcttttgcaa ttcattcaat ttggcatcca
     1141 tcttgctaga tgtggttgag gagtctgtcg tgcccgtctg cgcgagcgcg aggcaggttg
     1201 tgagcagcag cgcggctgcc agaagcaaaa gccgcatctg cttaatggaa aacgcggaaa
     1261 acgcggcgcg aaatgggaca ccgggcacgt agacctccag gatgagcgcg atggataaaa
     1321 tgcgcgctac gatttcgacg aggcgtaaat tttactccaa ttcgatgctg ttttttgcgc
     1381 gaccgaccga tttcggctat aaatcagggc tactccgcaa ctcggcaatc atgtcgacga
     1441 ggccgcaatg agcgcgatgt ccggcacggg aagaggacag aactcagagc agagccatcg
     1501 tcgccaaacc ggctcaatga aagcgatgtt tggatttttg accgcacaaa aggattccgg
     1561 cgatccgctg caaagcgcga aagccgcggc cgcatggctc cggcagctgc cggcgctcga
     1621 cgtcatcggg cggcagcagc acgtgattca ggctctcgac gcggtgcgcg gaacccagcg
     1681 gccgatcgac ctgaaccgca ttaccgctat cgaattcgtc gacgctgcgc tcggcgccga
     1741 tcgtcgccag ctgatcaagc agtacatcga gaacatcgag acttcgccga agctggccga
     1801 gcgcatctgg caggcgctgt gggagatgag tcaggcgttc accctggcct atcagcaggc
     1861 gctcgaaagc gcgctgccgc aggcgggaaa cgcgcgctgg cgcgcggctc tgccgctatt
     1921 gttcgtacgc ttcgttcact ttcacggcac cgacgccaag cttcgcgtat tcaagtacga
     1981 gcgctggatt ccggccaaat ggattgagct gcatcaggcc tatctccgcg cgtgcgagat
     2041 gcaatgcgat cggcaggcgg tggtgctgcc ggccgcaggc cagggcgcgc agcagtggac
     2101 ggttgagcag gaatacctgt acgtgcttct cgtgcatcag ctcaacaccg gcaacctcaa
     2161 cccgaccgaa atcgattggg caagctcgca gctgcgagca tggacccggc gcctcgaact
     2221 cgagcaaatt ccccgatcgc tggaaggatt tttcgtcgat ctggcaggac gcgaaggcct
     2281 gatccggcga accggcaacg atcgcggctc ggtgcttcgc tacctcgaca cgacgccgct
     2341 ggccgacggt ctcgagcgcg cgatcaccgc gctgcgcgac gccgagctca ccgatcaggg
     2401 tccggtcgcc gcgatcaatc agcaacgcct gtcggtgctg cgcaagatcc agcccgccgt
     2461 ctcgccgacg tcggagaccg aattgcggcg cgacccgcgt gtcgcagtcg cagtgtcggc
     2521 gcgcgtgcgc atcggtctgt cgcggatctg ccaggacctg ggtgcgcagg cggcgggcga
     2581 gaacggcggt gaagtcggca cgggaaccga gcagatcgaa gtgtttgccg tcgcgggtgc
     2641 gccgcgcatc aggcgcaagg cctccgaaga cgattcgctg gcagtgagtc tttcatcgtg
     2701 gaccgacccg acgtgggaaa tcaaggaccg cagcgttgcc ggactgcgca tcgccgcgac
     2761 cggcggcatc ggccagagcc tgacactggg gaccttggtc gcggtgcgcc aatccgacgc
     2821 cggcggatgg ttgctcggcg tcgtccggcg tctgaacaag gtttcgaacg aggaagtcga
     2881 agccggcgtc aatatcatcg cggagcgcgt cgtcgccgtg accctggcag cgaaacggcg
     2941 cgcgcacgag gacatgggct acgtcatcaa cggactcgac atgtcgacga tgggcgagcg
     3001 cttcgagggc ctgtatctgc cgccgccttc gcgtcccgac aagccgctgg cgatgaagac
     3061 gatcatcgtg ccgacctccg aatatgccga aggacgcaac gtgctgctga ccacggcgcg
     3121 gtccgtgtac acggtgtcga tgcgccacct ggtcgagcag cgtccggact ggagctgggc
     3181 gacgatacag ctcgtcgaca agaaggtgcg cgaggaagcc gcataagccc gcacggcgca
     3241 gcgggatagc gacctaatcc agcggccgga acttgagggt caggttg
//
LOCUS       DATANS010000819         2387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_248839, whole genome shotgun sequence.
ACCESSION   DATANS010000819 DATANS010000000
VERSION     DATANS010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248839"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..949
                     /locus_tag="VHT22_12165"
     CDS             71..949
                     /locus_tag="VHT22_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX3138361.1"
                     /translation="MVERILGIILPVFSVILLGYFYARRANPDMTWVNRINMHVLAPA
                     LIFSALASKDFNLVVNRVLIVGAIGVVLGSGLLAWPFARLLHEDPRTFVPPMMFNNCG
                     NMGLPLAVLAFGQPGLSAMVAIFTISNLLHFTLGVHIVNRHAAFWPIFRNPMVIATFV
                     GFAFALVHPPAPDWLMLAIRMTGDAMIPLMLISLDVRMATVSWGGWNLGVIGALVCPL
                     TGIAMSALLAPVLGLDATQAGLLILFGCLPPAVLNFMVAEQYNQEPGKVASIVLIGNG
                     ASIVFVPIALSLALRQ"
     gene            complement(943..1710)
                     /locus_tag="VHT22_12170"
     CDS             complement(943..1710)
                     /locus_tag="VHT22_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3138362.1"
                     /translation="MVTYDFTGRTAIITGGAGGFGRAIAARLHSSGAAIALWDRDAAG
                     VAAAAQLLGERALGFAVDTTDEAAVTAAAKAVVKRFDRIDFLINNAGILGPVSNTWEH
                     STDDFRRVLDINLTGTFICCRVIVPLLLANTPDPRTGQRGHIVNVSSIQAKEGMPRAA
                     AYAASKSGIVSLTKTLGKELAESGILVNTVTPAIVATPMAEGISKEREKEILARIPMR
                     RFGTVEEIAAQIAWLCSEDCSFATGAVFDLSGGRATY"
     gene            1854..>2387
                     /locus_tag="VHT22_12175"
     CDS             1854..>2387
                     /locus_tag="VHT22_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HEX3138363.1"
                     /translation="MDARTIDVAARQLVAARTARRPGARLPESCRPTDADAGLAIQRR
                     VGEILDLAIGGWKCSVPTPERKIAAAPIFAPTIISVAPCPIVPIAGKARVEPEIAFVL
                     ARDLPARATPYDEADVRAAIGEARLVLELMGPRYVDPAAVTFPELLADSIANQGLFAG
                     PVLSNPHGLSLDSFAIVI"
BASE COUNT          389 a          785 c          775 g          438 t
ORIGIN      
        1 ggcgccgatt ttctttcgat cttcgccgcc attccgattc gcgtgacgag aaatcgagcc
       61 tgacgccgtc atggtcgagc gcatcctcgg catcatcctg ccggtattca gcgtcatcct
      121 gctgggctac ttctacgccc gccgcgccaa tccggacatg acctgggtca atcgaatcaa
      181 catgcacgtg ctggcgccgg cgctgatttt cagcgcgctg gcgagcaagg acttcaatct
      241 tgtcgtcaat cgcgtgctga tcgtcggcgc catcggcgtg gtgctcggct cgggcctgct
      301 cgcgtggcct ttcgcgcggc tgctgcacga ggatccgcgc acgttcgtgc cgccgatgat
      361 gttcaacaac tgcggcaaca tgggcctgcc attggcggtg ctggcattcg gccagccggg
      421 gttgtcggcg atggtcgcga tattcacgat ctccaacctg ctgcacttca cgcttggagt
      481 acacatcgta aaccgccacg cggcattctg gccgatcttc aggaacccga tggtgatcgc
      541 caccttcgtc ggcttcgcct tcgcgctcgt ccacccgccg gcgcccgact ggctgatgct
      601 cgccatccga atgaccggcg acgcgatgat cccgctgatg ctgatctcgc tggacgtgcg
      661 catggcgacc gtcagctggg gcggctggaa cctcggtgtc atcggcgcgc tggtctgccc
      721 gttgaccggg atcgcgatgt cggccctgct cgcacctgta ctgggcctgg acgcgacgca
      781 agcggggctg ttgatcctgt tcggctgcct gccgccggcg gtgctcaact tcatggtcgc
      841 cgagcagtac aaccaggagc cgggcaaggt ggcttcgatc gtcctgatcg ggaatggcgc
      901 gtcgatcgtt ttcgtgccga tcgcgctgtc gctggcgttg cgtcagtagg tcgctcgtcc
      961 gccggaaaga tcgaacactg caccggtggc gaacgagcag tcctccgagc aaagccacgc
     1021 gatctgcgcg gcgatctctt cgacggtgcc gaagcgccgc atcgggatcc gcgccaggat
     1081 ctccttctca cgctccttcg agatgccttc ggccatcggc gtcgccacga tcgcgggcgt
     1141 caccgtgttc accaggatgc ccgattcggc gagctccttg cctagcgtct tggtcagcga
     1201 gacgataccc gacttcgacg ccgcgtaggc ggcggcgcgc ggcatgccct ccttggcctg
     1261 gatcgacgag acgttgacga tgtgcccgcg ctggccggtc ctcgggtccg gcgtattggc
     1321 gagcagcagc ggcacaatga cccggcagca gatgaaagtt ccggtcaggt tgatgtcgag
     1381 cacgcggcga aaatcgtcag tgctgtgttc ccaggtgttg gacaccgggc cgagaattcc
     1441 ggcgttgttg atcaggaaat cgatgcggtc gaaccgcttc acaaccgcct tggcggcagc
     1501 agtgacggcg gcttcgtcgg tcgtgtcgac agcaaacccg agcgcgcgct cgccaaggag
     1561 ctgcgcggcc gccgcgacgc cggcagcatc gcgatcccac agcgcgatcg ctgcccccga
     1621 cgaatgcagg cgcgccgcga tcgcccgtcc gaagccgccg gcgcctcccg tgatgatggc
     1681 agtgcgtccg gtaaaatcgt atgtgaccat cgaggcaccg cgagcaaggg atacgcgtga
     1741 tcggccaagc gacgggacgc cgggttgaca ggcgctgtga gcgcaattat acgatggtag
     1801 gcgccaagcc ctcgttggcg cgagaaacgc ggacccaagc gaaagatccc ccgatggacg
     1861 caagaacgat cgacgtcgcg gcgcgacaac tggtggctgc ccgcaccgcg cggcggcccg
     1921 gggccagatt gcccgaatca tgccgtccca cggacgccga cgcaggcctc gcgatacagc
     1981 gccgcgtcgg agaaatcctc gatctggcga tcggcggctg gaaatgctcg gtgcccacac
     2041 cggagcgaaa gatcgccgca gcgcctattt ttgcgccgac gatcatcagc gtggcgccct
     2101 gcccgatcgt gccgatcgcg ggcaaggcgc gcgtcgaacc cgagatagcg ttcgtgctag
     2161 cacgcgatct gccggcgcgc gcgacgccgt acgacgaggc cgacgtgcgt gcggcgatcg
     2221 gtgaagcgag gctggttctc gagctgatgg gaccgcgcta tgtcgatcct gcggcggtca
     2281 cgtttcccga gctgctggcc gacagcatcg ccaaccaggg actgtttgcc ggaccggtgt
     2341 tatccaatcc gcacgggctt tcgctcgact ctttcgccat cgtgatc
//
LOCUS       DATANS010000820         2503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_797304, whole genome shotgun sequence.
ACCESSION   DATANS010000820 DATANS010000000
VERSION     DATANS010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797304"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VHT22_12180"
     CDS             <1..320
                     /locus_tag="VHT22_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX3138364.1"
                     /translation="AALDSEVVDKLKNVSKLELVLVTGHTDRIGSQAYNQRLSERRAD
                     TVRDYLVSRGVARDKIETLGMGKTQPIPGVVCDQKNMKELIACLAPNRRVEVEVKGES
                     VKR"
     gene            402..1733
                     /locus_tag="VHT22_12185"
     CDS             402..1733
                     /locus_tag="VHT22_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HEX3138365.1"
                     /translation="MQTVDLRLDPRWVVPVVPRGALAAHSIIIDAGRIVAVVPAAEAD
                     RDYEAAEHLRLATHALLPGLINAHTHAAMSLLRGSADDVALAPWLQDHIWPLEKAFLS
                     PEFVHDGTLLAAAEMLAGGVTCCNDQYFFPDAAARAYQASGMRAMLGLAVLDFPSAYA
                     VNADGYLQSGLAVRDKWKHEPLLSFSLAPHAPYTVGDASWKKIVVYARQLDLPIQTHL
                     LETGDERSRSESEFGIDPLRRLHGLGVTGPGFIAVHGTHLKAADIELLVAHACHIVHC
                     PTSNLKLASGVAPVAELVRRGVNVALGTDGAASNNRLDVFGEARLAALLAKGDSGDAG
                     AIPAADALQMATLNGARALGLEREVGSLEPDKQADVIAVDLGAVAHAPCYDPVSHLIH
                     VTGRDQVTDVWVAGRRVVAGRRLTTVDTDELAARVRFWQDRVQSQQTSPAK"
     gene            1790..2503
                     /gene="ubiG"
                     /locus_tag="VHT22_12190"
     CDS             1790..2503
                     /gene="ubiG"
                     /locus_tag="VHT22_12190"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEX3138366.1"
                     /translation="MAEIIDNADPAELAKFSALAHRWWDPTSEFKPLHDINPLRLGHI
                     AAQCGGFAGKSVLDVGCGGGILAEAMAEAGARVVGIDLSEAALAVARLHQLESKSSVS
                     YRMIAAEALADERPSDFDLVTCMELLEHVPDPASMVAACAKLVRPGGTVICSTINRNP
                     KSYLFAIVGAEYVLKLLPRGTHDYARLLKPAELVGFARRARLELVDLTGMTYNPLART
                     YRLEPDTSVNYIATFRRDA"
BASE COUNT          431 a          856 c          789 g          427 t
ORIGIN      
        1 aggcagcgct ggacagcgag gtcgtcgaca agctcaagaa tgtgagcaag ctcgaactcg
       61 tcctcgtcac cggccatacc gaccgcatcg ggtcgcaagc gtacaaccag cgcctgtcag
      121 agcgccgcgc cgataccgtg cgcgactatc tggtcagccg tggcgtcgca cgcgacaaga
      181 tcgagacgct cggcatgggc aagacgcaac cgatcccggg tgtggtttgc gatcagaaga
      241 acatgaagga actgattgcc tgcctcgccc cgaaccgccg cgtcgaagtc gaagtcaagg
      301 gcgaatccgt caagcgctaa agcagcgctg cacgcggaaa aagccccgcc caagtgcggg
      361 gctttttctt tcccgctttt cgatccattc gattacacgc gatgcaaacc gtcgatctgc
      421 gactcgatcc gcgctgggtg gtgcctgtcg ttccgcgcgg cgcgctggcc gcgcattcga
      481 tcatcatcga cgccggacga atcgtcgccg tcgtcccggc cgcggaggcc gaccgcgact
      541 acgaggcggc cgagcacttg aggctggcca cgcacgcgct gctgccgggc ctgatcaacg
      601 cgcacacgca tgcggcgatg agcctgctgc gcggcagcgc cgacgacgtg gcactggcac
      661 cgtggctgca ggatcacatc tggccgctcg aaaaagcctt tctctcgccg gaattcgtgc
      721 acgatggaac gctgcttgcc gcggcggaaa tgctcgccgg tggcgtcacc tgctgcaacg
      781 atcagtattt ctttcccgac gcggctgcgc gcgcctacca ggccagcggc atgcgcgcga
      841 tgctcggcct ggcggtgctc gattttcctt cggcgtacgc cgtgaatgcc gatggctacc
      901 tgcaatcagg cctcgcagtg cgcgacaagt ggaagcacga gccgctgttg agcttttcgc
      961 ttgcgcccca tgcgccgtac acggtcggcg atgccagctg gaagaagatc gtcgtctatg
     1021 cacggcaact cgatctgcca atccagacgc acctgctgga gacaggcgac gagcgctcgc
     1081 gcagcgaatc cgaattcggc atcgatccgt tgcggcgctt gcatggtctg ggcgtgaccg
     1141 gcccgggctt tatcgcggtg cacggcactc atctcaaggc cgccgacatc gaattgctcg
     1201 tagcgcacgc ttgtcacatc gtgcattgcc cgacgtccaa cctcaaactc gccagcggcg
     1261 tcgcgccggt ggcggaactg gtgcgccgcg gcgtgaacgt cgcgctcggc actgatggcg
     1321 cagccagcaa caatcgactc gacgtgttcg gcgaggcgcg gctcgcggca ctgctcgcca
     1381 agggcgacag cggcgacgcc ggcgcgatcc ccgcagccga tgcactgcag atggccaccc
     1441 tgaacggcgc acgcgcgctc ggtctcgagc gcgaagtcgg ctcgctcgag ccggacaagc
     1501 aggccgatgt gatcgccgtc gatctgggcg cggtagcgca cgccccctgc tacgatccgg
     1561 tttcgcacct gatccacgtc accggtcgcg accaggtcac cgacgtctgg gttgccggcc
     1621 gtcgcgtcgt ggccggacgc cggctgacga ccgtggatac cgacgagctc gccgcgcgcg
     1681 tccgcttctg gcaggaccgg gttcagtcgc agcaaacgtc gccggcgaag tagaatccac
     1741 attctgccgg ctggatcggc accctcctcc ggcgttccgc atcgttcgca tggctgaaat
     1801 catcgacaac gccgacccgg ccgaactcgc caaattcagc gcgctcgcgc atcgctggtg
     1861 ggacccaacc agcgagttca agcccttgca cgacatcaac cccttgcgcc tcggccatat
     1921 cgcagcgcaa tgcggcggct tcgccggcaa gtcggtgctc gatgtcggct gcggcggcgg
     1981 catccttgcc gaagcgatgg ccgaggcagg cgcgcgcgtg gtcgggatcg atctgtcgga
     2041 agctgcactg gctgtcgccc ggctgcatca gctcgaatca aagtcgtcgg tgtcgtatcg
     2101 catgatcgcc gccgaagcgc tggccgatga gcgcccttcg gatttcgatc tggtgacctg
     2161 catggagctc ctcgaacatg tgccggaccc ggcgtcgatg gtcgcagcct gcgcaaagct
     2221 cgtgcggccc ggcggcaccg tcatctgctc gacgatcaac cgcaatccca agtcgtacct
     2281 gttcgccatc gtcggcgcgg aatacgtgct caagttgctg ccgcgcggca cgcacgacta
     2341 cgcgcgcttg ctgaagcctg ccgagctggt cggtttcgcc cgccgcgcca ggctcgagct
     2401 cgtggacctg accggcatga cttacaaccc gctcgcgcgg acctaccgac tcgagcccga
     2461 cacgtcggtc aattacatcg cgacgtttcg ccgcgatgcc tga
//
LOCUS       DATANS010000821         2651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_125402, whole genome shotgun sequence.
ACCESSION   DATANS010000821 DATANS010000000
VERSION     DATANS010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125402"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VHT22_12195"
     CDS             <1..219
                     /locus_tag="VHT22_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX3138367.1"
                     /translation="LLLHVYRQPDRFHDKVSTAMTQRLETLKPDASFAELLAVLNHDY
                     VAIIVHEGRFFGLITRYDLLTHLRRSLP"
     gene            216..1412
                     /locus_tag="VHT22_12200"
     CDS             216..1412
                     /locus_tag="VHT22_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEX3138368.1"
                     /translation="MNDSTVKSAATATQGFSTRAIHAGQEPDPSTGAIMTPIYATSTY
                     VQTSPGVHKGYDYARTGNPTRTALEACIASLENGARGFAFASGLAGMATLIDALDAGA
                     HIVASDDLYGGTYRLFENVRKRSANLRVSFVDMADPQRLRAAIGADTRMIWIETPSNP
                     LLKLIDLAAIARIARERDLIAVADNTFASPWIQRPLELGFDVVVHSATKYLNGHSDMI
                     GGAAVIGGEARHAALAERLAYLQNAVGGIIGPFDSFLALRGLKTLSLRMERHCANALE
                     LAQWLVEQPEVSRVHYPGLPSHPQHALARRQMRAFGGMVTIVLKTDLAGTKRFLENCR
                     LFALAESLGGVESLIEHPALMTHGSIPEPMRATLGIGDSLVRLSVGIEDVDDLRRDLR
                     DALDAI"
     gene            complement(1486..2277)
                     /locus_tag="VHT22_12205"
     CDS             complement(1486..2277)
                     /locus_tag="VHT22_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX3138369.1"
                     /translation="MYAIEYQRAKSVADAAAALARTGGKALAGGQSLVGAMKLRLANP
                     GTVVDLSGIAELKGIKKEGDAIAIGALTTHAEVASSAVVKQAIPALAALAEGIGDRQV
                     RNRGTIGGSIANNDPAADYPAAVLALNATVVTNKRKIAADDFFKGMYETALGAEEIIT
                     AVSFPVPKKAAYVKFPNPASRFALVGVFVAQTATAVRVAVTGASSHVHRSSALEAALA
                     KTFTADTAKAVKIPADSLNTDLHGSAEYRAHLVSVLTARAVIAAG"
     gene            complement(2290..>2651)
                     /locus_tag="VHT22_12210"
     CDS             complement(2290..>2651)
                     /locus_tag="VHT22_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020831969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX3138370.1"
                     /translation="NPMIVEGQVHGGVAQGIGQALLENCVYDKQSGQLLTGSYMDYAM
                     PRADDLPSFKVATKVTPCTHNPLGAKGCGEAGAIGAPAAMMNAVHDALSAAGVKYIDM
                     PATPHRVWSALQSARRS"
BASE COUNT          440 a          885 c          848 g          478 t
ORIGIN      
        1 ctgctgctgc acgtctacag gcagcccgat cgattccacg acaaggtttc gacggcgatg
       61 acgcagcgac tcgagacttt gaagcccgac gcgagcttcg ccgaattatt ggccgtgctg
      121 aatcacgatt acgtcgcgat catcgtgcac gagggccgtt tcttcggtct cattacccgc
      181 tatgatctgc tgacgcacct gaggagaagc ctgccgtgaa cgattcgacc gtcaagtccg
      241 ccgccacggc gacacagggg ttctccacgc gcgcgattca cgcgggccag gagcccgatc
      301 catcgaccgg cgcgatcatg acgccgattt acgcgacctc gacctatgtg cagaccagcc
      361 ccggcgtgca caagggctac gattacgcgc gcaccggcaa cccgacgcgg accgcgctgg
      421 aagcatgcat cgccagcctc gaaaacggcg cgcgcggatt cgccttcgcc tcggggctgg
      481 caggcatggc gacgctgatc gacgccctgg acgcgggtgc gcatatcgtc gccagcgacg
      541 atctgtacgg tgggacttat cggctgttcg agaacgtgcg caagcgctcg gccaacctgc
      601 gcgtcagctt tgtcgacatg gccgacccgc agaggctgcg cgcggcgatc ggcgccgata
      661 ccaggatgat ctggatcgag acgccgagca atcctttgct caagctgatc gatctggctg
      721 cgatcgccag gatcgcgcgc gagcgcgatc tgatcgcggt cgccgacaat acgtttgcca
      781 gcccctggat tcagcgcccg ctcgagctcg gcttcgatgt cgtcgtgcat tcggcgacca
      841 aatatctgaa cgggcattcg gacatgatcg gcggggccgc ggtgatcggc ggagaagccc
      901 gtcatgcggc gctcgccgag cgcctcgcct atctgcagaa cgcagtcggc ggcatcatcg
      961 gcccgttcga cagctttctt gcgttgcggg gcctgaagac gctgtcgctg cgcatggagc
     1021 ggcattgtgc caatgcgctc gagctcgcgc agtggctggt cgagcagccc gaggtctcgc
     1081 gcgtccatta tccggggctg ccttcgcatc cgcagcacgc gctggcgcgg cgccagatgc
     1141 gcgccttcgg cggcatggtc acgatcgtgc tgaagaccga tctcgccggg accaagcgct
     1201 tcttggaaaa ctgccggctg ttcgcgctgg ccgagagtct gggcggcgtc gagagcctga
     1261 tcgagcaccc agcgctgatg acgcacggct cgatccccga gccgatgcgc gcgacgctgg
     1321 gcattggcga ttcgctggtg cggctatcgg tcggcatcga ggacgtcgat gacctgcgcc
     1381 gcgacctgcg cgacgcgctc gacgcgatct gaccaccggc gacgggcaat cgacgtccgg
     1441 gttttcgcta gcgcgaagcc catcccctgc ccggcccaac gtccgctaac ccgcggcgat
     1501 caccgccctc gccgtaagca ccgacaccag gtgggcgcga tattcggccg agccgtgcaa
     1561 gtcggtgttg agcgaatcgg ccggaatttt caccgccttg gccgtgtcgg cggtgaaagt
     1621 ctttgccaat gcagcctcga gcgcactcga gcgatggacg tgtgaggacg caccggtcac
     1681 cgcgacgcgc acagcggtcg cggtctgcgc gacgaagacg ccgaccaggg cgaagcgcga
     1741 cgccgggttg ggaaatttga cgtaggcggc ctttttcggc accggaaaac tgaccgcggt
     1801 aatgatctct tcggcgccca gcgcggtttc gtacatgccc ttgaaaaaat cgtcggcggc
     1861 gatcttgcgc ttgttggtca ccacagtcgc attcagcgcc agcaccgcgg ccggatagtc
     1921 ggccgcagga tcgttgttgg cgatcgagcc gccgatcgtt ccgcggtttc tcacctgcct
     1981 gtcgccgatg ccttcagcga gcgcggccag tgccggaatc gcctgcttga cgaccgcgga
     2041 cgatgcgacc tccgcatgcg tcgtcaacgc cccgatggcg atcgcgtcgc cttctttctt
     2101 gatgcctttc aattccgcaa tgccggacag atcgactacg gttcccggat tggcgaggcg
     2161 cagcttcatc gcgccgacca ggctctgacc accggccaga gccttgccac ccgtccgggc
     2221 aagtgccgcg gcggcgtcgg cgaccgattt cgcgcgttgg tattcgatgg cgtacatgcg
     2281 gatctctcct taagaacgtc gcgccgactg cagcgcgctc cagacgcggt gcggcgtcgc
     2341 aggcatgtcg atgtatttga ccccggccgc ggacaaggca tcatgcacgg cgttcatcat
     2401 cgccgccggc gcgccgatcg cacccgcctc gccgcacccc ttggcgccga gcggattgtg
     2461 cgtgcacggg gtgaccttgg tcgccacctt gaacgacggc agatcatcgg cgcgcggcat
     2521 cgcgtagtcc atgtagctgc cggtcagcag ctggccgctt tgcttgtcgt agacgcaatt
     2581 ctcgagcagc gcctggccga ttccctgcgc gacaccgccg tgcacctgcc cttcgacgat
     2641 catcggattg a
//
LOCUS       DATANS010000822         1711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_837662, whole genome shotgun sequence.
ACCESSION   DATANS010000822 DATANS010000000
VERSION     DATANS010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837662"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VHT22_12215"
     CDS             <1..895
                     /locus_tag="VHT22_12215"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX3138371.1"
                     /translation="ARGTTAAALRARGWRMQTQQGLISALANVAETPSTLGPQDLVIV
                     AVKGPSLASVATSIAPLLGPDTAVLTAMNGVPWWFFHGFGTAYRDLQLRSVDPGGRID
                     AALPARRIIGCVVHASCSTPEPGLVRHHGGQRLIVGEPAGEPAARTHAIAAQLTAAGF
                     DVEVSRCIQRDIWYKLWGNMTMNPISALTGATMDRILDDELVKRFCLAAMSEAAAIGA
                     RIGCPIEQSGEDRIAVTRRLGAVKTSMLQDVEAHRQVEIDVLLSAVREIGERVEVATP
                     ALDALLGMARLQARVQGLYPS"
     gene            983..>1711
                     /locus_tag="VHT22_12220"
     CDS             983..>1711
                     /locus_tag="VHT22_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3138372.1"
                     /translation="MNDKKFASQADLEAKKVSFDKLSDHAYAYTAEGDPNTGVVVGDD
                     AIMVIDTQATPVMAQDVIRHIRTVSDKPIRYVVLSHYHAVRVLGASGYGAQHVIASRD
                     TYDLIVERGEADMKSEIERFPRLFQAVESVPGLTWPTLVFEKRMTLWVGKLQVEIMQI
                     GRGHTKGDTVVWLPQERILFSGDLVEYQTTPYTGDAYLSDWPATLDAIAALKPAKLVP
                     GRGAALQNEAQVAAGLDGTRAFITE"
BASE COUNT          305 a          540 c          577 g          289 t
ORIGIN      
        1 cgcccgtggc acgacggccg cagcgctgcg cgcgcgcggc tggcggatgc aaacgcagca
       61 aggattgatc agcgcactgg cgaacgtcgc cgagactccg tcgacgctcg gtccgcagga
      121 tctggtcatc gtggcggtca agggtccatc gctggccagc gttgcgacga gcatcgctcc
      181 gctgctcggc cccgatacgg cggtgctcac ggcgatgaac ggcgtgccct ggtggttctt
      241 tcacggtttt ggcaccgcgt atcgcgactt gcagctacgc tcggtcgatc ccggcggccg
      301 catcgacgcc gcgctgccgg cgcgacggat catcggttgc gtcgtccatg cgtcgtgctc
      361 gacccccgag cccggcctgg tacggcatca cggcggccag cgcctaatcg tcggtgagcc
      421 ggctggcgag ccggcggcgc gcacccacgc gattgccgcg caactgactg ctgccggatt
      481 cgacgtcgaa gtctcgaggt gcatccagcg cgacatctgg tacaagctgt ggggcaacat
      541 gacgatgaat ccgatttccg cattgaccgg cgcgacaatg gaccgcatcc tcgacgacga
      601 gctcgtgaag aggttctgtc ttgctgcgat gagtgaggcc gcggcgatcg gcgcgaggat
      661 cggctgtccg atcgaacaaa gcggcgagga ccggatcgcc gtgacgcgcc ggttgggcgc
      721 ggtcaaaacg tcgatgctgc aggacgtcga ggcgcacagg caggtcgaga tcgacgtgtt
      781 attgagcgcc gtgcgtgaaa tcggcgagcg ggtagaggtc gccacgccgg cgctcgatgc
      841 gctgctggga atggcgcggc tgcaggcgag agtgcagggc ctctacccgt cgtagcaccg
      901 cgttgtgcgg cccgcggcaa tccctcctac aatgggccat cgccgccgcc gcggcgcccg
      961 tttgatgcac aggacatttg cgatgaacga caagaaattc gcttcccagg ctgacctgga
     1021 agccaagaag gtgagcttcg acaagctgtc cgaccacgcc tatgcgtaca ccgccgaggg
     1081 tgatccgaac accggcgtcg tcgtcggtga tgatgcgatc atggtcatcg atacgcaggc
     1141 cacgccggtg atggctcagg acgtcatccg ccacatccgt acggtcagcg ataagccgat
     1201 ccgctatgtc gtgctttcgc actatcacgc ggtgcgcgtg ctcggcgcgt cgggctacgg
     1261 ggcgcagcac gtcatcgcca gccgcgacac ctacgatctg atcgtcgagc gcggcgaagc
     1321 cgacatgaaa agcgagatcg agcgttttcc gcgactgttc caggcggtcg aatcggtgcc
     1381 cgggctgact tggccgacct tggtattcga aaagcggatg acgctctggg tcgggaagct
     1441 gcaggtcgag atcatgcaga tcggacgcgg ccatacgaag ggcgacactg tcgtctggct
     1501 gccgcaggag cgcatcctct tttccggcga cctggtcgaa tatcagacga caccgtacac
     1561 aggagatgct tatttgagcg actggccggc gacgctcgac gccatagcag cgctgaagcc
     1621 ggcgaagctc gtgcccggcc gcggtgccgc attgcaaaac gaagcccagg tcgctgcggg
     1681 cctcgacggc acacgcgcgt tcatcaccga g
//
LOCUS       DATANS010000823         4112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_125719, whole genome shotgun sequence.
ACCESSION   DATANS010000823 DATANS010000000
VERSION     DATANS010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125719"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..495
                     /locus_tag="VHT22_12225"
     CDS             64..495
                     /locus_tag="VHT22_12225"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138373.1"
                     /translation="MLEPVSLPQTWFVVLSPPIEVSTRAMFAAPELTRQSHSAKMAVF
                     SEGYGRNDLQAVASARFPEIAGCIAALSRAAPASAARMTGSGASVFAAFTSERAAQQA
                     LSRMPRATEGTGETAGGNAGETAGFVARALNRHPLWSFASR"
     gene            522..596
                     /locus_tag="VHT22_12230"
     tRNA            522..596
                     /locus_tag="VHT22_12230"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:554..556,aa:Gln,seq:ttg)
     gene            659..1609
                     /locus_tag="VHT22_12235"
     CDS             659..1609
                     /locus_tag="VHT22_12235"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX3138374.1"
                     /translation="MAFERMRIFTGSANPKLAEAVVRHMNISLGRCIVGRFSDGEVMV
                     ELLENVRGRDVFILQSTSQPTNDNLMEILVMVDALKRASAGRITAAIPYFGYARQDRR
                     PRSARVPITAKVVADMLTTVGVNRVMTMDLHADQIQGFFNIPVDNIYATPVLLGDLWK
                     QNYDNLLVVSPDVGGVVRARAIAKRLDSDLAIIDKRRPKANVAEVMNIIGDVAGRACV
                     IMDDIVDTANTLCKAASALKANGAQKVLAYCTHPVLSGGAVARIAESELDEIVVTDTI
                     ALSDEARACKRIRQLSCAQLLAETMTRISNEESVSSLFTE"
     gene            1891..2631
                     /locus_tag="VHT22_12240"
     CDS             1891..2631
                     /locus_tag="VHT22_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEX3138375.1"
                     /translation="MQFEFTAFPRGTEGRGASRRLRRTGRAPGIVYGGAEKPEPIELD
                     HNALIHALNKEAFHSSILTMKLGEAAQRVLLRDVQMHPFRHEVLHIDFQRVDENRKIH
                     MKVPLHFINAELAPAVKTTDAIISHVLTELDVSCLPKDLPAFVEVDLSPLAVGQSIHV
                     SDLKLPAGVTAVTRPGPSPVVATAVVPRIVVEVEEVAAPTAEAVAGEAAAAPAEGAKP
                     GDKSAEKGGEKPAEKGADKAPAKKDDKK"
     gene            2724..3353
                     /gene="pth"
                     /locus_tag="VHT22_12245"
     CDS             2724..3353
                     /gene="pth"
                     /locus_tag="VHT22_12245"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX3138376.1"
                     /translation="MAHSFRLIVGLGNPGREYQETRHNAGFWWIEHLAGTLQASFRPE
                     SKFQAQVAAARGGDGDLRLAMPQTFMNRSGLAVASLARFYRIAPAEILVAHDELDLKP
                     GVVKLKQGGGNAGHNGLKDIAAALGSGDFWRLRLGIGHPRDGALSEQEVADYVLHPPP
                     GEERLLIADAIARALAIWPWLARGETERAMHELHTAPRPPPAAEGPARS"
     gene            3350..>4112
                     /gene="ychF"
                     /locus_tag="VHT22_12250"
     CDS             3350..>4112
                     /gene="ychF"
                     /locus_tag="VHT22_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015420320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX3138377.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTRAGIAAENYPFCTIEPNVGIV
                     EVPDPRLAALAAIARPERVLPAVVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIAH
                     VVRCFEDPNVVHVAGKIDPVSDIETINTELALADLAAVDKQMNRYAKAARAGGDKEAK
                     RLMAALEKIVPALNDARPARSVDLYQEELAVLKPLFLLTMKPTMYVANVADGGFEQNP
                     LLDAVRRHAATEGAPVVPVCASLEAEIADLDGDDKL"
BASE COUNT          742 a         1348 c         1353 g          669 t
ORIGIN      
        1 cttggcgccg acgttccgtt ttttgtattc ggcgaaaccg cgcgcgcaca gggcatcggt
       61 gaaatgctcg agccggtcag cctgccgcaa acctggttcg tcgtgttgtc gccgccgatc
      121 gaggtctcga ccagggccat gttcgctgct cctgaattga cacgacagag ccactccgcg
      181 aaaatggccg tcttttccga gggatatggc cgtaacgacc tgcaagcggt tgcgtctgcg
      241 cggttcccgg agatcgccgg ctgtattgcc gcgctgtcgc gcgcggcgcc agcgtcggca
      301 gcgcggatga ccggatcggg agcgagcgtg ttcgccgctt tcacttccga acgcgccgcg
      361 cagcaagcgc tctcgcggat gccgcgcgcg acagaaggca ccggtgaaac cgccggtgga
      421 aacgccggag aaacggccgg atttgttgct cgcgcgctga accgccaccc gctgtggtcg
      481 tttgcctcac gttgatgttc ccgcgcgcga acggtttccc ttggggagtc gccaagtggt
      541 aaggcaccgg attttgattc cggcattcgt aggttcgatc cctacctccc cagccaccaa
      601 cacgtcgggc aggccctccg ttcgggcggc ctgcccacgt cttccatagg cggcgacgat
      661 ggcgttcgag cggatgcgaa tcttcaccgg gagcgccaac ccgaagctcg ccgaggccgt
      721 cgtccggcac atgaacatca gcctaggccg atgcatcgtc ggtcgattct ccgacggcga
      781 ggtgatggtc gagctgctgg aaaacgtgcg cggccgggac gtgttcatac tccagtcgac
      841 ctcgcagccg accaacgaca acctgatgga aatcctggtg atggtcgacg cgctgaagcg
      901 ggcctccgcc gggcgcatta cggccgcgat accttatttc ggctatgcgc ggcaggatcg
      961 ccgcccccgg tcggcgcgcg tgccgatcac ggccaaagtc gtcgccgata tgctgacgac
     1021 ggtcggcgtc aatcgtgtga tgacgatgga ccttcacgcc gaccagatcc agggattttt
     1081 caatatcccg gtcgacaaca tctacgccac gccggtgctg ctcggcgatc tgtggaaaca
     1141 gaactatgac aatctgctgg tcgtctcgcc cgacgtaggc ggcgtcgtgc gcgcgcgggc
     1201 gatcgcgaag cggctcgact ccgatctggc gatcatcgac aagcgcaggc cgaaggccaa
     1261 cgtcgccgag gtgatgaaca tcatcggcga tgtcgcgggt cgcgcctgcg tgatcatgga
     1321 cgacatcgtc gacaccgcca acaccctgtg caaggcggca tcggcactca aggccaacgg
     1381 cgcacagaag gttctggcct actgcacgca cccggtcctg tcgggcggcg ccgtcgcgcg
     1441 catcgccgaa tcggagctgg acgagatcgt ggtgaccgac accatcgcgc tgtccgatga
     1501 agcgcgcgcc tgcaagcgca tccggcagct ttcctgcgcg caactccttg ccgagacgat
     1561 gacgcgcatc tccaacgagg aatcggtcag ctcgctgttt accgagtaag tctgtctgag
     1621 cgcggagcag gggccccgcg caagcgggga tgcgaccgcg aaggcaggct gcggccgaca
     1681 ctcggtgtgc gagttgcgca acgcctccga ggaggccgcg gcgcgcgctt gcgcctagag
     1741 atctgcgcga atgcgggctt tgcgtccacg gctcaattca ggttacaatt caaggctttc
     1801 cccgtcgccg cctgccggcg gcattgtcgc catcgcggac gaccgcagcg cataagcgca
     1861 cggaaggccg cagacaacag gagcaacatc atgcagttcg aatttaccgc ctttccccgc
     1921 ggcaccgaag gacgaggcgc gagccgccgg ctgcgccgca ccggtcgcgc gccgggaatc
     1981 gtctatggcg gcgccgagaa gcccgaaccg atcgagctcg atcacaatgc cctgatccac
     2041 gcgctcaaca aggaagcgtt tcattcgtcg atcctgacga tgaaactggg cgaggccgcg
     2101 cagcgggtgc tgctgcgcga cgtgcagatg catccgttcc ggcacgaagt gctgcacatc
     2161 gatttccagc gggtcgacga gaaccgcaag atccacatga aagtgccgct gcactttatc
     2221 aacgccgagc tcgcgccggc agtcaagacg accgacgcca tcatcagcca cgttctgacc
     2281 gagctcgacg tctcctgcct gcccaaggac ctgcccgcat tcgtcgaagt cgacctgtcg
     2341 ccgcttgcgg tcgggcaatc gatacacgta tcggatctga agctgcctgc aggcgtgacc
     2401 gcggtgaccc ggccgggtcc aagccctgtc gtggcgacgg cggtcgtgcc gcgcatcgtc
     2461 gtcgaggtcg aggaagtcgc ggcgccgacg gccgaagcag tggcgggcga agctgcggct
     2521 gcgccggcgg aaggggccaa gcccggcgac aagtccgcgg aaaagggcgg cgagaagccc
     2581 gcggaaaaag gtgccgacaa ggcgccggcc aagaaggacg acaagaaata agcgcccggg
     2641 gtacgcgaag ggcccgccgg tgcttgtctc ggcgggcctt tttgctgttt cgctccggcc
     2701 cttggccaat ctgagctgat gccatggcgc attcgttccg ccttatcgtc ggcctcggca
     2761 atccgggacg cgaataccaa gagacgcggc acaacgccgg tttctggtgg atcgaacacc
     2821 tcgccggcac actgcaggcg agcttccggc ccgaatccaa atttcaggcg caggtagccg
     2881 cggcgcgcgg cggcgacggc gatctgcgcc ttgcgatgcc gcagacgttc atgaatcgca
     2941 gcggacttgc ggtcgcctcg cttgcgcgct tctatcggat cgcgcccgcc gagatcctgg
     3001 tcgcacacga cgagctcgat ctgaaaccgg gggtggtcaa gctgaagcag ggcggcggca
     3061 atgccggaca caacggcctg aaggacatcg ccgccgcgct gggcagcggc gacttctggc
     3121 gactgcgact cggcatcggc catccgcgcg acggtgcgct gtccgaacag gaagttgccg
     3181 attacgtgct gcatccgccg cccggcgagg agcggctgct gatagcagac gcgatcgcgc
     3241 gggcgctggc gatatggccg tggctcgcgc gcggcgagac cgagcgcgcg atgcacgagc
     3301 tgcacaccgc gccgcggccg ccacccgctg ccgaaggacc ggcgagatca tgagcctcaa
     3361 atgcggaatc gtcggtctgc cgaacgtcgg caagtcgacg ctgttcaacg cgctgacccg
     3421 cgccggcatc gcggcggaaa attacccgtt ctgcacgatc gagcccaacg tcggcatcgt
     3481 cgaggtgccc gacccacggc tcgcggcgct cgccgcgatc gcgcgccccg agcgcgtgct
     3541 tccggccgtg gtggaattcg tcgacattgc gggactcgtc gccggcgcct cgaaagggga
     3601 aggactcggc aaccagtttc tggccaacat acgcgagaca gatgccatcg cccatgtcgt
     3661 gcgctgtttc gaggatccga acgtcgtgca cgtcgccggc aaaatcgatc cggtctcgga
     3721 tatcgagacg atcaacaccg agcttgcgct cgccgacctc gccgccgtcg acaagcagat
     3781 gaaccggtac gccaaagcgg cccgggccgg tggcgacaag gaggcgaagc ggctgatggc
     3841 agcgctggaa aagatcgtcc ctgcgttgaa cgatgctcgg cccgcccgct cggtcgacct
     3901 ctatcaggaa gagctggcgg tgttgaagcc gctgttcctg ctgaccatga agccgacgat
     3961 gtacgtggcc aacgtcgccg atggcggctt cgagcagaat ccgctgctgg acgcggtgcg
     4021 gcgccatgct gccaccgaag gcgcgcccgt ggtgccggtg tgtgcgtccc tcgaggccga
     4081 gatcgccgat ctcgacggcg acgacaagct cg
//
LOCUS       DATANS010000824         3277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_627551, whole genome shotgun sequence.
ACCESSION   DATANS010000824 DATANS010000000
VERSION     DATANS010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627551"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="VHT22_12255"
     CDS             <1..1184
                     /locus_tag="VHT22_12255"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HEX3138378.1"
                     /translation="DHAAALFNMERAGYVYSRIANPTCAVLEERIAALEGGVGAIAVA
                     SGQAALHLAIVTLLGAGSHIVASRALYGGSHNLLDYTLPRFGIATTFVDPRDADAWRA
                     AIRPETRLLFGETLGNPGLDVLDVPRISALAHDHGLPLLVDSTFTTPYLMRPFEHGAD
                     LVYHSATKFLSGHGVVIGGLLVDSGRFDWDRPGASAGKFPTLTEPYAGFHDMVFTEES
                     TTAAFLLRARREGIRDFGACMAPMTAFQILQGIETLPLRMQRHVDNTRRIVAFLAAHP
                     LVESVAYPELPAHPDHALAQRLLPLGCGAVFSFAIRGSREQGRKFIESLKLFSHLANV
                     GDAKSLVIHPASTTHFRMAAAELRASGITEGTIRLSVGLEHPDDLIEDLSRALHAAGK
                     A"
     gene            1221..2021
                     /locus_tag="VHT22_12260"
     CDS             1221..2021
                     /locus_tag="VHT22_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3138379.1"
                     /translation="MYVTVEGDPVYVYTGARAFDRARSTIVFVHGAANDHSVWGLQAR
                     YFAHHGNNALAIDLPAHGKSGGSARTSIGSIADWLTALLDALGVDRAVLVGHSMGALA
                     ALDMTARHPERVRALALLGPAAPMVVSDVLLDAAEAHDHLAYELMTGWTFSATSHLGG
                     NRQPGIWMTNVALRLFERSRPGVLHADLEACHRYADGLAAAKTIHCPVLLVFGQRDLM
                     VPPKNARPLQDALTGKRIVTIPECGHSMMAEAPDVVLDTLREFIASLS"
     gene            complement(2024..2575)
                     /locus_tag="VHT22_12265"
     CDS             complement(2024..2575)
                     /locus_tag="VHT22_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX3138380.1"
                     /translation="MGAAPPVCNFGWAAPDFELVGTDGKRYRRDSARGDNGLLVMFIC
                     NHCPYVKAVLDKMVRDAGELRDHGIGSVAICSNDAADYPEDSFDNMKALATRMRFPFP
                     YLWDETQSVAKAYGAICTPDFFGFNVALELQYRGRLDDAGRAPKPDAPRELYEAMLQV
                     ARSGRGPAQQTPSVGCSIKWRTA"
     gene            2636..>3277
                     /locus_tag="VHT22_12270"
     CDS             2636..>3277
                     /locus_tag="VHT22_12270"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX3138381.1"
                     /translation="MTTVTPRFHCPLPLAEGAEIALPDAAAHHAARVLRLAAGDAVTL
                     FSGDDGEFAARILRIEPRGVHVQIGAAHAADRESPLAVTLVQGLAAADRMDYAIQKAV
                     ELGVQSIQPVTMARSVARLDEARAAKRALHWRQITIAACEQCGRNQLPRLHPLVGFDA
                     WLGASSEAPLRLLLAPDGDVTLGSLGAVPDGPVEILVGPEGGLTPEETSAALRR"
BASE COUNT          495 a         1124 c         1083 g          575 t
ORIGIN      
        1 ccgaccatgc ggcggcactg ttcaacatgg agcgcgcagg ctacgtctat tcgcgcatcg
       61 ccaatccgac ctgcgcggta ctcgaggagc gcatcgccgc gctcgaaggc ggcgtcggcg
      121 ccatcgcggt ggccagcggc caggcggcgc tgcatcttgc catcgtgacc ctgctgggcg
      181 cgggctcgca catcgtcgcc tcgcgggcct tgtacggcgg ctcgcacaat ctgctcgact
      241 acacgctgcc gcgcttcggc atcgcgacga cgttcgtcga tccacgcgac gcagatgcct
      301 ggcgcgcggc gatccgtccg gaaacgcggc tgctgttcgg cgagacgctc ggcaaccccg
      361 gcctcgacgt gctcgacgtg ccgcgcatct ccgcgctggc gcacgaccac gggctgccgc
      421 tgctggtcga ttcgacgttc acgacgccgt atctgatgcg cccgtttgag cacggcgcgg
      481 acctggtcta ccattcggcg accaagtttc tgtcgggtca tggtgtcgtg atcggcggcc
      541 tgctggtcga cagcggccgg ttcgactggg acaggccggg cgccagcgcc ggcaagttcc
      601 cgacgttgac cgagccttac gccggctttc acgacatggt gttcaccgag gaatcgacga
      661 ccgccgcgtt cctgctgcgc gcgcggcggg aaggtattcg cgatttcggt gcttgcatgg
      721 cgccgatgac cgctttccag atcctgcagg ggatcgagac cttgccgttg cgcatgcagc
      781 gccatgtcga caacacgcgg cggatcgtcg ccttcctggc ggcgcatccg ctggtcgaat
      841 cggtcgccta ccccgagctc ccagcccacc ccgatcacgc gctcgcgcag cggctgctgc
      901 cgctcggctg cggcgcggta ttcagctttg caattcgcgg cagccgcgag cagggacgca
      961 aattcatcga gtcgctgaag cttttctcgc atcttgccaa cgtcggcgac gcgaagtcgc
     1021 tggtcatcca tccggcgagc acgacgcatt ttcggatggc ggccgcggag cttcgagcgt
     1081 ccggcatcac cgaaggcacg atccggttgt cggtcggcct cgagcatccg gacgacctga
     1141 tcgaggatct gtcgcgcgcg ctccacgccg ccggcaaggc ctgactcgcg cctgattcga
     1201 tcaagtcgcg ccgcaaagtc gtgtacgtca ccgtcgaagg cgatcccgtc tatgtctata
     1261 caggcgcgcg cgcgttcgat cgcgcgcggt cgacgatcgt gttcgtgcac ggcgcggcca
     1321 acgaccacag cgtctgggga ttgcaggcgc ggtatttcgc ccaccatggc aataatgcgc
     1381 tggccatcga tttgcccgcg catggcaaaa gcggcggcag cgccaggacg tcgatcgggt
     1441 cgatcgccga ctggctgacc gcgttgctag atgccttggg cgtcgatcgc gcggtgctgg
     1501 tcgggcacag catgggcgcc ttggcggcgc tcgacatgac ggcgcgccat ccggaacgcg
     1561 tgcgcgcgct cgcgctgctg ggcccggccg cgccgatggt ggtcagcgac gttctgctcg
     1621 acgctgcgga agcccacgac catctggcgt acgagctgat gaccggatgg accttttccg
     1681 cgaccagcca cctcggcggc aaccggcagc ccggcatctg gatgaccaac gttgcgctgc
     1741 gcttgttcga gcgctcgcga cccggtgtgc tccatgccga cctcgaggcc tgccatcgtt
     1801 acgccgacgg tctcgccgcc gcgaagacga tccattgtcc ggtgctgctg gtgttcggcc
     1861 agcgcgacct gatggtcccg ccgaagaacg cccgaccgct gcaggatgcg ctgacgggca
     1921 aacggatcgt gacgattccg gaatgcggcc acagcatgat ggccgaagcg cccgacgttg
     1981 tgctcgatac gctgcgtgaa tttatcgcaa gtctctcgtg acgttacgcg gtgcgccact
     2041 tgatcgagca gccgacgctc ggcgtctgct gtgccggtcc ccgcccgctg cgcgcgacct
     2101 gcagcatcgc ctcatagagc tcgcgcggcg catcgggctt cggtgcgcgg cccgcgtcgt
     2161 cgagccggcc gcgatactgc aattcgagcg caacgttgaa cccgaagaaa tcgggcgtgc
     2221 agatggcgcc gtacgccttc gccaccgact gcgtctcgtc ccacagatag ggaaacggga
     2281 agcgcatccg tgtggccagc gccttcatgt tgtcgaacga gtcctccgga taatcggccg
     2341 cgtcgttgct gcagatcgcc acgctgccga tgccatggtc gcgaagctcg ccggcgtcgc
     2401 gcaccatctt gtccagcacc gccttgacat acgggcagtg gttgcagatg aacatgacca
     2461 gcagtccgtt gtcgccgcgt gccgagtcgc ggcgatatcg tttgccgtcg gtgccgacga
     2521 gctcgaagtc gggcgcagcc caaccgaagt tgcacaccgg tggcgcggcg cccatgtcgg
     2581 ttctcctgtc caatgctgtg gcaaaatgct agcacatgcg aggcaacttc cagtcgtgac
     2641 cactgtgacg ccgcgcttcc attgcccgct gccgttggcc gagggcgccg agatcgcgct
     2701 ccccgatgcc gccgcgcatc atgcggcgcg cgtactgcgc cttgccgcgg gtgacgccgt
     2761 cacgctgttc agcggcgatg acggcgaatt cgccgcgcgc attctgcgca tcgagccgcg
     2821 cggcgtgcac gtccagatcg gagcggcgca tgcggccgac cgcgaatcgc cgctggcggt
     2881 gaccctggta cagggactcg ccgccgccga tcggatggat tatgcgatcc agaaggccgt
     2941 cgagctcggc gtccaatcga ttcagccggt cactatggcg cgcagcgtcg cccggctgga
     3001 cgaagcgcgc gcggccaagc gggctttgca ttggcgacag atcaccatcg ccgcctgcga
     3061 gcaatgcggc cgcaaccagc tgccccgctt gcacccgctg gtcggcttcg acgcatggct
     3121 cggtgcatcg tcggaggcgc ctttgcggct gctgctcgca ccggatggcg acgttacgct
     3181 cggcagtctg ggcgcggtgc ccgacggccc ggtcgaaatc ctggtcggtc ctgagggtgg
     3241 cctgacgccg gaagaaacct ctgccgcact gcgccgc
//
LOCUS       DATANS010000825         2201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_838310, whole genome shotgun sequence.
ACCESSION   DATANS010000825 DATANS010000000
VERSION     DATANS010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838310"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VHT22_12275"
     CDS             complement(<1..616)
                     /locus_tag="VHT22_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3138382.1"
                     /translation="MNSRKLIVGLALAGVAAAGGYGLYHVGLQEGMKASATLTPNAAV
                     TAETNSVPLKAGDVDPTTGKKVLYWQDPMAPGQRFEKPGKSPLMDMQLVPVYADEAGT
                     DPAAVAISPRIQQNLGVRTAEVQQGAMSVTMEAVGSAAYNERDVAVVQARSNGFLERL
                     YVRAPLDRVRRGQPLAELYVPDWVAAQEEYLSARRIRANADGKTF"
     gene            complement(613..1929)
                     /locus_tag="VHT22_12280"
     CDS             complement(613..1929)
                     /locus_tag="VHT22_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX3138383.1"
                     /translation="MDFSRTRNAAAQRIATLVAMALLIASSHAFAAEAPLTLADAQRR
                     AVERSRQLAAQDFTVAASREMAVAAAQLPDPVATLGIYNLPIDGPDAFSLTRDFMTMR
                     SIGVMQEWTREEKRRAKADRFEREADRSLVERAARAAAIRRETALAWLDRYYAEAQSS
                     LIAEQTRQSRLEVEAAETAYRTGRGNLGDVLAARSSLSALDDRASELGRRVGAAKIAL
                     ARWVGQAAMGPLADKPPSDSVGLDPGKLDSELEHHPQIAALASQEEVAAAEVRLAQAN
                     KKADWSVQFMYSQRGPAYSNMVSLAVSVPLQWDHAHRQDRELAAKLALLDQARAERED
                     MLVSHVAEVRAMVAEWENDRERSARYERELVPLAAERTQAMLASYRGAKASIVDVLLA
                     RRGEIDIRLQALQLEAETARLWAQLNFLGSVDDAEMSTPNRYRKLP"
     gene            complement(2003..>2201)
                     /locus_tag="VHT22_12285"
     CDS             complement(2003..>2201)
                     /locus_tag="VHT22_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138384.1"
                     /translation="TCWAHCYADPQVDAHDYAPAPLVAPQTALLVRLADPGLPNLVPA
                     QWLLPVMAAPPPQLRFSRFLI"
BASE COUNT          349 a          725 c          721 g          406 t
ORIGIN      
        1 caaaggtctt gccatccgca ttcgcgcgaa tgcgtcttgc tgacagatac tcctcctgcg
       61 ccgcgaccca atcgggaacg tagagctccg ccagcggctg accgcgacgc acccggtcga
      121 gcggcgcgcg cacatacagg cgctcgagga aaccgttgct tcgtgcctgg acgaccgcga
      181 catcgcgctc gttatacgcg gcgctgccga cggcttccat ggtcacgctc atcgcgcctt
      241 gctgtacctc cgccgttcgc acgccgaggt tctgttgaat gcgcgggctg atagcgaccg
      301 ctgccgggtc cgtgccggcc tcatcggcgt agacgggaac gagctgcatg tccatcaaag
      361 gcgacttgcc cggtttttcg aatcgttgtc ctggcgccat cgggtcctgc caatacagaa
      421 ccttcttgcc cgtcgtcgga tcgacgtcgc cggcttttag tggaacggag tttgtctcgg
      481 ccgtcacggc agcgttgggc gttagggtgg ccgacgcttt catgccttcc tgcaggccaa
      541 catggtaaag gccgtagccc ccggcggcgg cgacgccggc gagcgccagc ccgacgatga
      601 gttttctgct gttcatggca atttcctgta tcggtttggg gtcgacatct ccgcgtcatc
      661 gacggacccc agaaagttga gctgcgccca tagccgggcg gtctccgcct cgagttgcag
      721 tgcctgcagg cggatatcga tttcgccacg gcgagcgagc aggacatcga cgatgctggc
      781 tttggcgccc cggtacgagg cgagcattgc ctgcgttcgc tcggcggcca gcgggaccag
      841 ttcgcgctcg tagcgggcgc tgcgttcgcg atcgttttcc cattcggcaa ccatcgcgcg
      901 tacctcggcg acatgggaga ccagcatgtc ctcgcgctct gctcgcgcct gatccagcag
      961 cgcgagcttg gcggccaact cgcggtcctg acggtgggcg tggtcccatt gcaaggggac
     1021 cgacactgca agcgaaacca tgttcgaata cgccggcccg cgctggctgt acatgaattg
     1081 cacactccag tcggccttct tgttcgcctg cgccaatctg acttccgcgg cggcgacttc
     1141 ctcctggctc gccagcgccg cgatttgcgg atgatgctcg agctccgaat cgagctttcc
     1201 gggatcgagc ccgacggagt cgctcggcgg cttgtccgcc agcggcccca tggcggcttg
     1261 gcccacccag cgtgccagtg caatcttggc cgcgcctacg cggcggccga gctcacttgc
     1321 ccggtcatcg agtgcgctca gcgaactccg cgcggcgaga acgtcaccga gattgccgcg
     1381 ccctgtgcga taagccgttt cggcggcttc gacctcgagc cgggactgcc gggtttgctc
     1441 ggcgatgagc gacgactgcg cttcggcgta atagcgatcc agccatgcca gcgctgtctc
     1501 gcgccggatg gcggcagcgc gggcggccct ctctaccaga gatctgtcgg cttcgcgctc
     1561 gaaacggtcg gccttcgccc ggcgtttttc ctcgcgggtc cattcctgca tcacgccaat
     1621 gctccgcatg gtcatgaaat cgcgggtcag gctgaacgca tcgggcccgt cgatcgggag
     1681 gttgtatatg cctagcgtcg cgacgggatc gggcagctgc gcggcggcta ccgccatctc
     1741 gcgcgatgcg gcgactgtga agtcctgcgc cgccagttgt cgcgatcgct cgacggcgcg
     1801 acgctgcgcg tcggcgagcg tcagcggtgc ttccgccgcg aatgcatgcg acgacgcaat
     1861 cagcagtgcc atcgcgacca gcgtcgcgat gcgttgagcg gccgcgttac gggtacgaga
     1921 aaagtccatt cgatctccac gtccacacga aaaggcgccg gcgcacggac ggccgtgcgc
     1981 cacattcagc gtgagcggaa gatcagatca ggaaccgact gaagcgaagc tggggaggtg
     2041 gcgctgccat gaccggcagc agccattgcg cgggaacaag gttcggaagg cctgggtccg
     2101 caagccggac gagcagcgcc gtctgcggcg cgaccaatgg ggccggcgcg tagtcgtgcg
     2161 cgtcgacctg tggatcagcg tagcagtgcg cccagcaggt a
//
LOCUS       DATANS010000826         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_628672, whole genome shotgun sequence.
ACCESSION   DATANS010000826 DATANS010000000
VERSION     DATANS010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628672"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VHT22_12290"
     CDS             <1..228
                     /locus_tag="VHT22_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3138385.1"
                     /translation="AGRGEGADGSPLLPMVLLPDSGQDESPPMALVPAGVFQLEQAIS
                     LHRDKSVGTAVLTKLVDQGPGFELFEYVAVA"
     gene            212..583
                     /locus_tag="VHT22_12295"
     CDS             212..583
                     /locus_tag="VHT22_12295"
                     /inference="COORDINATES: protein motif:HMM:NF021369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEX3138386.1"
                     /translation="MSPSRDGSGRWRRATAASVLALALLELLWELWLAPLKPGGSWLA
                     LKAVPLLGLTPGVAQGRVRARQWTLLVLPWYVAEGVVRAFSESGRQALCAGAAAALAV
                     VALAAGLGWFRAQSRAPRPPV"
     gene            complement(588..>1521)
                     /gene="ispH"
                     /locus_tag="VHT22_12300"
                     /gene_synonym="lytB"
     CDS             complement(588..>1521)
                     /gene="ispH"
                     /locus_tag="VHT22_12300"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX3138387.1"
                     /translation="LANPRGFCAGVDRAIAIVEQALTRFGAPIYVRHEVVHNKFVVDD
                     LKAKGAIFVDELADVPKGSTVVFSAHGVSRAVRDEAAARGLTVFDATCPLVTKVHVEV
                     AKMHANGHEIVMIGHAGHPEVEGTMGQADGGIHLVDSVGDVDRLVVRDPSQLAYVTQT
                     TLSVDDAATIVAALKRRFPTIIGPRKDDICYATQNRQDAVKFLAPQCDVVIVVGSPNS
                     SNSNRLREVAAHRGIPAYMIDRAEELQPEWIEGKRRVGVTAGASAPEILVQQVIERLR
                     ELGAAKVRELDGAVESVMFPLPRGLAAHGERGTH"
BASE COUNT          216 a          508 c          537 g          260 t
ORIGIN      
        1 gcgggccgcg gcgaaggcgc ggacggctcg ccgctgctgc cgatggtgct gctgccggac
       61 agtggtcagg acgagtcgcc gccgatggcg ctggtcccgg ccggagtgtt ccaactcgag
      121 caggcgatat cactgcaccg cgacaagtcg gtcggcaccg cagtgctgac caagctggtc
      181 gatcaaggtc cggggttcga gctattcgag tatgtcgccg tcgcgtgacg gcagcggccg
      241 ctggcggcgc gcgaccgctg cgtccgttct cgcactggca ctgctggaat tgctctggga
      301 gctgtggctc gccccgctga agccgggcgg ctcgtggctc gcgctcaagg ctgtgccgct
      361 gctcggcttg acgcccggcg tggcgcaggg gcgcgtgcgc gcgcggcaat ggacgctgct
      421 ggtgctgccc tggtacgtcg ccgagggcgt cgtgcgcgcg ttcagcgaaa gcggccggca
      481 ggccttgtgc gccggtgcgg cggcggcgct ggcggtcgtt gcgctcgcag ccgggctcgg
      541 ctggtttcgt gcccagagcc gcgcgccgcg tcctccggtc tgaggagtca gtgcgtgccg
      601 cgctcgccgt gcgcggcgag cccccgcggc agtggaaaca tcacggattc gacggcaccg
      661 tccagctccc gaaccttggc ggcgccgagc tcgcgcagcc gttcgatcac ctgctggacc
      721 aggatttccg gcgccgaggc tcccgcggtg acaccgacgc gccgcttgcc ctcgatccac
      781 tcgggctgaa gctcttcggc gcgatcgatc atgtaggcgg gaataccgcg atgcgcggcg
      841 acctcgcgca accggttgga attcgagctg ttcgggctgc cgaccacgat gaccacatcg
      901 cattgcggag caagaaactt gaccgcgtcc tggcggtttt gcgtcgcata gcagatgtcg
      961 tccttcctcg ggccgatgat cgtgggaaaa cggcgcttga gcgccgcgac gatcgtggcc
     1021 gcgtcgtcga ccgacagcgt cgtctgcgtc acgtaggcga gctgcgaggg gtcgcggaca
     1081 acgaggcgat cgacgtcgcc gaccgagtcg accagatgga tgccgccgtc ggcctggccc
     1141 atcgtgccct cgacttcagg gtggcccgcg tggccgatca tcacgatttc gtgcccattg
     1201 gcgtgcatct tggcgacttc gacatgcacc ttcgtcacca gcggacaggt cgcgtcgaag
     1261 acggtcaagc cgcgcgccgc ggcttcgtcg cgcaccgctc gcgagacgcc gtgcgcgctg
     1321 aacacgacgg tgcttccctt cggaacatcc gcaagctcgt cgacaaatat cgcgcccttc
     1381 gccttgagat cgtcgacgac gaacttgttg tgcacgactt cgtgtcgcac gtagataggc
     1441 gcgccgaacc gggtgagcgc ctgctcgacg atcgcgatgg cccgatcgac gccggcgcaa
     1501 aatccgcgcg ggttggcgag t
//
LOCUS       DATANS010000827         1746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_628834, whole genome shotgun sequence.
ACCESSION   DATANS010000827 DATANS010000000
VERSION     DATANS010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628834"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VHT22_12305"
     CDS             complement(<1..892)
                     /locus_tag="VHT22_12305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX3138388.1"
                     /translation="MSTAPHGDDTAGLPPPSLEARVHGEQVLAHITAAIDEAGGWISF
                     ADYMGVVLYAPGLGYYSAGARKFGAAGDFVTAPEMTPLFGAALATPAADLIDRLPDGD
                     LIELGPGTGRLAADLLNALVAKERLPRRYRLLEVSADLAARQRECLRERAPALLPRVD
                     WIEILPPRWRGLMIANEVLDAVPPHLIARQRGAWFERGVSLIGGRLALADQPLKQGAL
                     RDAASAAFPPEGDYVSEINPAAQALIRSLAERCDAGMLLIIDYGFPATEYYHPQRASG
                     TLVAHYRHRAVHDPLFMPGLC"
     gene            940..1677
                     /locus_tag="VHT22_12310"
     CDS             940..1677
                     /locus_tag="VHT22_12310"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HEX3138389.1"
                     /translation="MQGKVVLITGGAKRVGAATCRRLHAAGAQLMLHYRESAGEARLL
                     QAELNHQRKDSVALIQADLLDLGKLQSLIDQTVQTFGKLDALINNASSFFPTPVGEIT
                     GENWDDLIGTNLRAPLFIAQAAAPALKKTQGAIVNITDIHAERPLKNYVVYSVAKAGL
                     VGLTRSLARELAPEVRVNAVAPGPILWPDDESFDELSRQRIISNTPLKREGTPDDIAK
                     AVHFLLFEATYVTGETINVDGGRHVAL"
BASE COUNT          315 a          577 c          582 g          272 t
ORIGIN      
        1 cgcacaatcc gggcatgaac agcgggtcgt gcacggcgcg atgacgatag tgcgcgacca
       61 gcgtgccgct cgcgcgttgc ggatgataat actcggtggc gggaaacccg tagtcgatga
      121 tcagcagcat gccggcgtcg cagcgctctg cgagactgcg gatcaacgct tgcgccgctg
      181 gattgatctc gctgacatag tcgccttcgg gcggaaacgc tgcgctggcg gcatcgcgca
      241 acgcaccctg cttcagcggc tggtcagcga gagcaagtcg accgccgata agactgacgc
      301 cgcgctcgaa ccatgcaccg cgctgccgcg cgatcaggtg cggcggaacc gcatcgagca
      361 cctcgttggc gatcatcagt ccgcgccagc gcggcggaag aatctcgatc cagtcgacgc
      421 gcggcaacag ggccggcgcc cgctcgcgca agcactcgcg ctgccgcgcc gcgagatcgg
      481 cgctcacttc gagcaggcga taacgccgcg gcagacgctc cttcgccacc agcgcattca
      541 gcagatcggc ggcgaggcga ccggtgccag ggcccagctc gatcagatcg ccgtccggaa
      601 gtcggtcgat gaggtcggcg gccggcgtgg ccagcgcggc gccaaacagc ggtgtcattt
      661 ctggtgcggt gacgaaatcg ccggcggcgc cgaacttgcg cgcgcccgcc gagtaataac
      721 cgagtcccgg cgcatacagc accacgccca tgtagtcggc gaatgagatc cagccgccgg
      781 cctcgtcgat cgccgccgta atgtgcgcca agacctgctc gccgtgcacg cgcgcttcga
      841 gcgaaggcgg cggcaatccg gcagtgtcat cgccgtgtgg agcggtggac atcgatgtgt
      901 taaattgggc gattcgagtc agtgtagccg gagcgccgcg tgcagggcaa agtggtcttg
      961 atcacggggg gtgccaaacg cgtcggcgcg gcgacttgcc ggcgcctgca tgccgcgggg
     1021 gcccagctga tgctgcatta tcgcgaatcg gccggagaag cgcggctgct gcaggccgag
     1081 ctcaatcacc agcgcaaaga ctcggtcgcg ctcatccagg ccgacctgct cgatctcggc
     1141 aagctgcagt cgctgatcga ccagacggtg cagaccttcg gcaagctcga cgcgctgatc
     1201 aacaatgcat cgtcgttctt cccgactccc gtgggcgaga tcaccggtga gaattgggac
     1261 gatctgatcg ggaccaatct gcgcgccccg cttttcatcg cacaggccgc ggcgcccgcg
     1321 ctgaagaaga cgcaaggcgc gatcgtcaac atcaccgaca tccacgccga gcgcccgctg
     1381 aagaactacg tcgtctacag cgtcgccaag gccggactgg tcggattgac gcgctcgctg
     1441 gcgcgcgagc tggcgcccga agtgcgcgtc aatgccgtcg cgcccggtcc gatcctgtgg
     1501 cccgacgacg aatcgttcga cgagctgtcg cggcaacgga tcatttcgaa cacgccgctc
     1561 aagcgcgagg gcacgcccga cgacatcgcc aaggccgtgc acttcctgtt gttcgaggcg
     1621 acctacgtga ccggggagac gatcaacgtc gacggcggga ggcacgtggc tttataggcg
     1681 ccgtagtcgg cgcgcccgaa agtgccgttt gttttctctt cgcggcgacc cgcagcgcga
     1741 gcgctt
//
LOCUS       DATANS010000828         2307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_250999, whole genome shotgun sequence.
ACCESSION   DATANS010000828 DATANS010000000
VERSION     DATANS010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250999"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VHT22_12315"
     CDS             <1..248
                     /locus_tag="VHT22_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX3138390.1"
                     /translation="VVGAEHVETDPTPEMGSEDFAFMLQAKPGCYVWLGAGSGPDTHN
                     IHSPHYDFNDRALAIGASYWVTLVEQQLAAAAAQKAA"
     gene            complement(261..1445)
                     /locus_tag="VHT22_12320"
     CDS             complement(261..1445)
                     /locus_tag="VHT22_12320"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEX3138391.1"
                     /translation="MTGAILVLNAGSSSLKFTLYASSAAGPRPLVRGQVEGLTTTPAF
                     VARDAQDRVVQQRDWPDRDALGQAGAVTYVLDFVRQQQSQQTLAAVGHRVVHGGEEFD
                     APVRIDAEVLARLERLVPLAPLHQPHNLAPMRVIAERAPTVPQVACFDTSFHRTQEPV
                     ARAFGLPSEITERGVRRYGFHGLSYEYIADVFPRFDPAAARGRVVAAHLGNGASMCAM
                     KAGRSIATTMGFTALDGLMMGTRAGALDPGVVLYLLDELEMDARAIERLLYHQSGLLG
                     VSGVSSDMRTLLSSDDPRAAFAIALFCYRVRRELGSLAAALGGIDAIVFTGGIGEHAA
                     SIRERICRDAQWLGVTLDEQSNAAGGPRISGELSRVSAWVIPTDEEAMIARHTFAALE
                     AG"
     gene            complement(1445..>2307)
                     /locus_tag="VHT22_12325"
     CDS             complement(1445..>2307)
                     /locus_tag="VHT22_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HEX3138392.1"
                     /translation="DRDSLLGAIEAARIGLIVPVLVGPSARIRATAKSEGVDLSAYRV
                     VSTEHSHAAAAAAVAMARTGEVEALMKGSLHTDELMSAVVPTEAGLRTARHISHVYIL
                     DVPTYPRLLMITDAAINITPDLADKADICRNAIDLAHVLGLAEPRVAILSAVETVNPR
                     IASTVDAAALCKMAERGQIVSGIIDGPLAFDNAISADAARIKHIESPVAGRADILLVP
                     DLESGNMLAKQLQYLAGAGSAGIVLGARVPIVLTSRADPVRDRLASTAVLKLLAHDRR
                     NKSAGSVAQV"
BASE COUNT          390 a          777 c          767 g          373 t
ORIGIN      
        1 cggtcgtcgg cgccgagcat gtcgaaaccg atccgacgcc cgagatgggc agcgaggatt
       61 tcgcgttcat gctgcaggcg aagcccggct gctatgtctg gctgggcgcc ggcagcggtc
      121 ccgacacgca caatattcac agcccgcatt acgatttcaa cgatcgggcg ctggcgatcg
      181 gcgccagcta ctgggtgaca ctggtcgaac agcaattggc cgctgccgcg gcgcagaagg
      241 cggcttagac gagcggctga tcagccggct tcgagcgcgg cgaatgtgtg ccgcgcgatc
      301 atcgcctcct cgtcggtcgg aatcacccac gccgaaacgc ggctgagttc gccgctgatc
      361 ctcggtccgc cggcggcatt cgactgctcg tcgagcgtga cgccgagcca ttgcgcgtcc
      421 cgacagatcc gctcccggat cgacgccgcg tgctcgccga tcccgccggt gaacacgatc
      481 gcgtcgatgc cacccagcgc ggcggccagc gaacccagct cgcgcctgac gcgatagcag
      541 aacagcgcga tcgcgaacgc cgccctgggg tcgtcgctgg agagcagcgt gcgcatgtcg
      601 ctcgaaaccc cggacacgcc gagcaggcct gactggtggt aaaggagccg ttcgatcgcg
      661 cgcgcgtcca tctcgagctc gtccagaagg tagagcacca cgccgggatc gagcgcgccg
      721 gcgcgggtgc ccatcatcag cccgtcgagc gcggtgaatc ccatcgtcgt ggcgatgctc
      781 ctgcccgcct tcatcgcgca catgctggca ccgttgccga ggtgcgccgc gacgacccgt
      841 ccgcgcgccg ccgcaggatc gaagcgcggg aatacatcgg cgatgtattc gtacgacagt
      901 ccgtgaaaac cgtagcggcg aacgccgcgc tcggtgattt ctgacggcaa tccgaatgcg
      961 cgtgcgaccg gctcctgcgt gcggtgaaac gaggtgtcga agcaggcgac ttgcggcacc
     1021 gtcggtgcgc gctcggcaat gactcgcatc ggcgccagat tgtgcggctg gtgcagaggc
     1081 gccagaggca caagccgctc gagccgcgcc aggacttcgg catcgatccg caccggcgcg
     1141 tcgaactcct cgccgccgtg cacgacgcgg tggccgacgg cggcaagcgt ctgctgactt
     1201 tgctgttgcc gcacgaagtc gagtacgtag gtgactgcgc cggcctgtcc cagcgcgtcg
     1261 cgatcgggcc aatcgcgctg ctgcacgacc cggtcctgcg catcgcgcgc gacgaacgcc
     1321 ggcgtcgtag tcagaccctc gacttggccg cgcacgagcg gccgcggacc ggcggcgctg
     1381 ctggcataca aggtgaattt caggctcgac gacccggcgt tcagcaccag gatcgcgccg
     1441 gtcatcaaac ttgtgccaca ctgcccgcgc tcttgttgcg ccggtcgtgc gcgagaagct
     1501 tgagcaccgc ggtcgaggcg agcctgtcgc gcaccgggtc cgcgcgcgag gtcagcacaa
     1561 tcggaacgcg ggcgccgagc acaattcctg cgctgcccgc gccggccaga tattgaagct
     1621 gcttggccag catattgccg gattcgaggt cggggacgag aaggatgtcc gcacggcctg
     1681 cgaccggtga ttcgatgtgc ttgatcctgg cggcatcggc gctgatcgcg ttgtcgaacg
     1741 ccagcggtcc gtcgatgatg ccactgacga tctgcccgcg ctcagccatc ttgcacagcg
     1801 ccgccgcatc gacggtcgag gcgattcgag ggttcaccgt ctcgaccgcc gagaggatgg
     1861 cgaccctcgg ctctgccaag ccgagcacat gcgccagatc gattgcgttg cgacagatat
     1921 cggccttgtc ggccaggtcc ggggtgatgt tgatcgcggc gtcagtgatc atcagcagcc
     1981 gcggataggt ggggacatcg agaatgtaca cgtggctgat gtgacgcgcg gtgcgcagtc
     2041 ccgcttccgt cggcacgacc gccgacatca actcgtcggt gtgcagactg cccttcatca
     2101 aggcttcgac ttcgccagtg cgtgccatcg ctaccgctgc ggcagccgca gcgtgactat
     2161 gttcggtcga cacaacccgg taggccgaca ggtcgacccc ttccgacttc gcagtcgcgc
     2221 gaatccgagc cgatgggccg accagcactg gcacgatcag cccgatcctg gcggcttcga
     2281 tggcgccaag cagagaatcg cgatcgc
//
LOCUS       DATANS010000829         1950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_251056, whole genome shotgun sequence.
ACCESSION   DATANS010000829 DATANS010000000
VERSION     DATANS010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251056"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VHT22_12330"
     CDS             complement(<1..459)
                     /locus_tag="VHT22_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HEX3138393.1"
                     /translation="MNKVYPDAKSALDGVLKDGMMIMSGGFGLCGIAETLSDAIRDSG
                     VKGLTVISNNAGVDGIGLSRLLETRQIKKMISSYVGENKLFAQQYLAGELELEFNPQG
                     TLAERIRAGGAGIPAFYTKTGVGTLIAEGKEVKEFDGEKYIMERGLFADLA"
     gene            741..>1950
                     /locus_tag="VHT22_12335"
     CDS             741..>1950
                     /locus_tag="VHT22_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX3138394.1"
                     /translation="MGQGMKRLGMPIAALFCVAVLALIGTSWFLNRDALRQAVEAQIR
                     DVTGLDLVVSGAIDVSVFPGSYVSFHNVGLKGGGTMDPALQVDVLTANLRLLPLLLRR
                     FEIADVMMLRPQIRVVRDGSGESNWTPFVERIARTMKPGAENQVSFSEIRIQDGVLKY
                     EDAANHVTEQLGDIDLSLAWPSISRSFAATGQFDWRGERVDGSISAGDFVAMLSGERS
                     GLKARIVSAPLKLAFDGSVANRTSLMMEGTVTVDSLSLRNALRWMGQPVPGSGGFGRF
                     ALKARANVVGASVALTNVNVELDGNVAEGVMTVANNGRQTLQATLAAGNLDFTPYIST
                     FRLLASGARDWNRQLFDLNALSATDLDMRLSAAKVTIGPTKLGRTAFGANLRNGALAL
                     SIGEAQMYGGI"
BASE COUNT          336 a          618 c          627 g          369 t
ORIGIN      
        1 ggccagatca gcgaacaggc cgcgctccat gatgtatttc tcgccgtcga attccttcac
       61 ttccttgcct tcggcgatca gcgtgccgac gccggtcttg gtgtagaagg ccgggatacc
      121 ggcgccgccg gcgcggatgc gttcggccag cgtgccctgc ggattgaatt cgagttccag
      181 ctcgccggcg aggtattgct gcgcgaacag cttgttctcg ccgacatagg acgagatcat
      241 cttcttgatc tgccgtgtct ccaaaagccg ggagaggccg atgccgtcga cgcccgcatt
      301 gttggagata accgtcaaac ccttgacgcc ggaatcgcgg atcgcgtccg acagggtttc
      361 ggcgataccg cacagaccga agccgccgga catgatcatc atgccgtcct tgagaacgcc
      421 atcgagtgcc gatttggcgt cggggtaaac cttgttcatg gagtctgacc tgatggaggg
      481 aacggccgaa tgccggcatt atctccgaat tattaggcga attcttcgcg gtgcgtcaat
      541 gacgggtgga ttatgccgcc gaaccgccta ccgggcgcca cctcggcctt gaaagcgtca
      601 agaaaaccct tcaagttgtg tgggttggcc gggcttgacc gttgcaccgc ctgccctgat
      661 caacatagac ccttaatcaa caaagtcccc gtagcatcaa cccgagttcg gtttagccag
      721 atccggatat gtcattgacg atgggccaag gaatgaagcg cctcgggatg ccgattgcgg
      781 cgctgttttg cgtggccgtg ctcgccttga tcggaacctc ctggttcctc aaccgcgacg
      841 cgctgcgcca ggcggtcgag gcgcagatcc gcgacgtcac cgggctcgat ctggtggtgt
      901 cgggcgcgat cgacgtttcg gtgtttccgg gcagctacgt ctcctttcac aatgtcgggc
      961 tgaagggcgg cggcaccatg gaccccgcgc tgcaggtcga tgtgctgacc gcgaacctgc
     1021 gcctgctgcc gctgctgctg cgccgcttcg agatcgccga cgtcatgatg ctgcggccgc
     1081 agatccgcgt cgtcagggac ggtagcggcg agagcaactg gacgccgttc gtcgaacgga
     1141 tcgcgcgcac gatgaagccc ggcgccgaaa accaggtgtc gttttccgaa atcaggattc
     1201 aggacggcgt gctcaaatac gaggacgccg ccaaccacgt caccgagcag ctcggcgaca
     1261 tcgatctgtc gctggcctgg ccgtcgatct cgcgctcgtt cgcggcgacc gggcaattcg
     1321 actggcgcgg cgaacgcgtc gacggctcga tctcggcggg cgacttcgtc gcgatgctgt
     1381 cgggcgagcg ctcgggcctg aaggcgcgca tcgtcagcgc gccgctgaaa ctggccttcg
     1441 acggctcggt cgccaaccgc accagcctga tgatggaagg cacggtgacc gtcgacagcc
     1501 tgtcgctgcg caacgcgctg cgctggatgg gacagccggt gcccggcagc ggcggcttcg
     1561 gccgtttcgc gctgaaggcg cgcgccaatg tcgtcggcgc ctcggttgcg ctgaccaacg
     1621 tgaatgtcga actcgacggc aatgtcgccg agggcgtgat gacggttgcc aataacggcc
     1681 gccagacgct gcaggcgacg ctcgccgcgg gcaacctcga tttcacgccc tacatttcca
     1741 ccttccgcct gctcgccagc ggcgcgcgcg actggaaccg gcagctgttc gacctcaacg
     1801 cgctgtcggc gaccgatctc gacatgcgcc tgtctgcggc caaggtgacg atcggcccga
     1861 ccaagctcgg ccgtaccgcg ttcggcgcca atttgcgtaa tggcgcgctg gcgctcagca
     1921 ttggcgaggc gcagatgtat ggcggtatcg
//
LOCUS       DATANS010000830         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_629094, whole genome shotgun sequence.
ACCESSION   DATANS010000830 DATANS010000000
VERSION     DATANS010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629094"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VHT22_12340"
     CDS             <1..666
                     /locus_tag="VHT22_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX3138395.1"
                     /translation="TDLAMVAVQGPNARAKAAKVIGKSADAVLGMKPFFSRQLERHFV
                     ATTGYTGEDGWEVILPAKEIAAFWAALRDAGVQQCGLGARDTLRLEAGMNLYGNDMDE
                     NLTPLESGLTWTVAFDPADRDFIGRAALEAQRRGGVPRKLVGLVLEDRGVLRSHQKVV
                     VPGAGEGEMTSGTFSPTLERSIGFARVPAATGEQVQVDIRGKLLAARVVKYPFVRNGK
                     ALI"
     gene            695..1093
                     /gene="gcvH"
                     /locus_tag="VHT22_12345"
     CDS             695..1093
                     /gene="gcvH"
                     /locus_tag="VHT22_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX3138396.1"
                     /translation="MSSIPSNLKYLDSHEWARVESDGTVTIGISDHAQGALGDLVFVE
                     VPEVGRALTKGGAAAVVESVKAASDVYSPVSGQVIANNSEALSATPELVNSDPYGQGW
                     LFKVKPSNSGELSSLLDAKAYEKVVEEAGH"
     gene            1095..>1577
                     /locus_tag="VHT22_12350"
     CDS             1095..>1577
                     /locus_tag="VHT22_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019569024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HEX3138397.1"
                     /translation="MPFIPHTETDVREMLAAIGAPSIDALFEEIPAALRIKSLDGIPE
                     ALTEMETGRLMQQRASRDGRPLNFLGAGAYEHHIPAAVWAIVTRGEFYSAYTPYQAEA
                     SQGTLQLLYEYQTMMASLTGMQVSNASLYDGASALAEACLMAVRAHRRSKSARILVPA
                     S"
BASE COUNT          297 a          508 c          524 g          248 t
ORIGIN      
        1 accgatctcg cgatggtcgc cgtgcaaggc ccgaacgcac gcgccaaggc agcgaaggtc
       61 atcggcaaga gcgccgacgc agtgctcggc atgaaaccgt tcttctcccg ccagctcgag
      121 cggcacttcg tcgcgacgac cggttacacc ggcgaagacg gctgggaagt catcctcccg
      181 gccaaggaaa tcgctgcgtt ctgggcagcg ctgcgcgacg cgggcgtaca gcaatgcgga
      241 ctcggcgcgc gcgacacgct gcgcctcgaa gcagggatga acttgtacgg caacgacatg
      301 gacgaaaacc tcacgccgct cgaatccggt ctcacgtgga ccgtcgcatt cgacccggca
      361 gatcgcgatt tcatcggccg cgcagcgctg gaagcgcaac gccgcggtgg tgtgccgcgc
      421 aagctggtcg ggctggtgct cgaagatcgc ggcgtgctgc gcagtcatca gaaagtcgtc
      481 gtgcccggcg ccggcgaggg cgaaatgacc agcggcacct tctcacccac gctggagcgc
      541 tcgatcggtt tcgcgcgcgt gccggcggcg accggcgagc aggttcaggt cgatatccgc
      601 ggcaagctcc tcgcggcgcg cgtcgtgaag tatccgttcg tgcgcaatgg caaagcattg
      661 atctaagccc tatcgaatca agacaggtac agccatgtcc tccattccca gcaatctcaa
      721 gtatctcgac tctcatgaat gggcccgcgt cgaaagcgat ggcaccgtga ccatcggcat
      781 ctccgatcac gcgcagggcg cgctcggcga cctggtgttc gtcgaagtgc cggaagtcgg
      841 ccgtgcgctg accaagggtg gcgctgccgc ggtagtcgag tccgtgaaag cggcgtccga
      901 cgtgtacagc ccggtgtcgg gtcaggtcat cgccaacaac agcgaggcgc tgagcgccac
      961 gcccgagctg gtcaacagcg atccgtacgg gcagggctgg ttgttcaagg tgaagccgag
     1021 caacagcggc gagctgtcga gcctgctcga cgcgaaagcc tacgagaagg tggtcgagga
     1081 ggctgggcac tgacatgccg ttcatcccgc acaccgaaac cgacgttcgc gagatgctgg
     1141 ccgccatcgg cgcgccgagc atcgatgcgt tgttcgaaga aattccggcc gcgctgcgca
     1201 tcaagtcgct cgacggcatt cccgaagcgc tgactgagat ggagacaggc cggctgatgc
     1261 agcaacgtgc atcgcgcgac ggccgtccgc tcaacttcct cggcgcgggt gcctacgagc
     1321 atcacatccc ggccgcagtc tgggcgatcg tgacgcgtgg cgagttctac agcgcgtaca
     1381 cgccgtatca ggcggaggcg agccagggca cgctgcagct cctgtacgaa taccagacga
     1441 tgatggcgag cctgacggga atgcaggtct ccaacgcatc gctgtacgac ggcgcctcgg
     1501 cgctggccga agcctgcctg atggcggtcc gcgcgcatcg caggtcgaag tcggcgcgca
     1561 tcctcgtgcc cgcgtcg
//
LOCUS       DATANS010000831         1827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Casimicrobiaceae bacterium isolate SMAG_U7025
            k141_629343, whole genome shotgun sequence.
ACCESSION   DATANS010000831 DATANS010000000
VERSION     DATANS010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955638
            Sequence Read Archive: SRR12917497
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Casimicrobiaceae bacterium (soil metagenome)
  ORGANISM  Casimicrobiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Casimicrobiaceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 05:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,472
            CDSs (total)                      :: 2,460
            Genes (coding)                    :: 2,444
            CDSs (with protein)               :: 2,444
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Casimicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629343"
                     /isolate="SMAG_U7025"
                     /isolation_source="soil"
                     /db_xref="taxon:2906524"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.8725 N 89.3578 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /gene="guaA"
                     /locus_tag="VHT22_12355"
     CDS             <1..1280
                     /gene="guaA"
                     /locus_tag="VHT22_12355"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018294408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX3138398.1"
                     /translation="TREFGYAAVRARGHGRLLEGIQDRSGADGQGLLDVWMSHGDKVI
                     SMPPGFALMASNDATPIAAMMDESRRFYGVQFHPEVTHTRQGKTILQRFVRDICGCLG
                     DWTMPAYVDEAIAKIRAQVGDDEVILGLSGGVDSSVAAALIHRAIGPRLTCIFVDTGL
                     LREGEAAQVMATFAEHLGVRVDMVDASEAFLRELAEVVDPEQKRKIIGREFVHVFQAE
                     AAKLRTGGIAAKWLAQGTIYPDVIESAGAKTGKAHTIKSHHNVGGLPETLHLKLLEPL
                     RELFQDEVRELGLALGLPRAMVFRHPFPGPGLGVRILGEVKREYADLLRRADAIFIDE
                     LREAGWYDRVAQAFAVFLPVRSVGVMGDGRTYEHVVALRAVETTDFMTAHWAPLPHEL
                     LARVSNRITNEVRGINRVVYDISGKPPATIEWE"
     gene            1292..1699
                     /locus_tag="VHT22_12360"
     CDS             1292..1699
                     /locus_tag="VHT22_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX3138399.1"
                     /translation="MKRENFSTGTRWEPLVGYSRAVRVGNRVFVSGTTATGPDGAIVG
                     KGDMYAQARQAIANIERALGTAGACLGDVVRTRMYVTDIAQWEAVGRAHGEAFRDIRP
                     ATAMLEIRRLIDADMLVEIEADAVIADAAGPAG"
BASE COUNT          301 a          590 c          658 g          278 t
ORIGIN      
        1 ggacgcggga attcggctac gccgcggtgc gggcgcgcgg acatggccgg ctgctcgagg
       61 gcatccagga ccgcagcgga gccgacgggc agggcctcct cgacgtatgg atgagtcacg
      121 gcgacaaggt gatctccatg ccgccgggct tcgcgttgat ggcgagcaac gatgcgacgc
      181 cgatcgccgc gatgatggac gaatcgcgtc gcttctacgg cgtgcagttc cacccggaag
      241 tcacgcacac ccggcaaggc aagacgatcc tgcagcggtt cgtgcgcgac atttgcggct
      301 gcctcggcga ctggacgatg ccggcctacg tcgacgaagc gatcgcgaag atccgtgcgc
      361 aggtcggtga cgacgaggtc atcctgggcc tttccggtgg agtcgattcg tcagtcgcag
      421 ccgcgctgat ccatcgcgcg atcggcccgc ggctgacctg catattcgtc gacaccgggc
      481 tgctgcgcga aggcgaggca gcgcaggtga tggcgacttt cgccgagcat ctgggcgtgc
      541 gcgtcgacat ggtcgatgcc agcgaagcct tcttgcgcga gctcgccgag gttgttgacc
      601 ccgagcagaa acgcaagatc atcggccgcg agttcgtgca cgttttccag gcggaagccg
      661 ccaagctacg gaccggagga attgcggcca agtggctggc gcaggggacc atctatccgg
      721 atgtgatcga atccgccggt gcgaagaccg gcaaggcgca tacgatcaaa tcgcatcaca
      781 acgtcggcgg cctccccgaa acgctgcatc tgaagctgct cgagccgctg cgcgagctct
      841 tccaggacga agtgcgcgag cttggcctgg ccctcggcct gccgcgcgcg atggtgttcc
      901 gccacccgtt tcctggcccc ggcctcggtg tgcgcatcct gggcgaggtc aagcgcgaat
      961 acgccgatct gctgcgccgc gccgatgcga ttttcatcga cgagctgcgc gaagcggggt
     1021 ggtacgatcg tgtcgcgcag gcgttcgcgg tgtttctgcc ggtccgttcg gtcggggtga
     1081 tgggcgacgg acgcacctac gagcacgtcg tggcgctgcg cgcagtcgag acgaccgact
     1141 tcatgaccgc ccactgggcg ccgctgccgc acgagctgct ggcgcgggtg tcgaaccgga
     1201 tcaccaacga agttcgcggc atcaaccgcg tcgtctacga catctccggc aaaccgccgg
     1261 caacgatcga atgggagtaa aggccgaccc gatgaaacgc gagaatttct ccaccggcac
     1321 gcgctgggaa ccgctggtcg gctattcgcg cgcggtgcgc gtagggaacc gcgtgttcgt
     1381 gtccggaacg acggcgacgg gcccggacgg cgcgatcgtc ggcaagggag acatgtacgc
     1441 gcaggcgcgg caggcgatcg ccaacatcga acgcgcgctc ggcacggccg gggcttgcct
     1501 cggcgacgtc gtgcgcacgc ggatgtacgt caccgacatc gcgcagtggg aggcggtcgg
     1561 ccgtgcgcac ggcgaagcct tccgcgacat ccgtccggcg acggcgatgc tcgagatccg
     1621 gcggctgatc gacgccgaca tgctggtcga gatcgaggcc gacgccgtca tcgccgacgc
     1681 tgccggcccg gcggggtgag cacgatggcc ggcccgcggc gcgatgccgc gccgtcgtcc
     1741 acggtgcgcg cggccgatcc ggcagcaagg tggatggcgg aggcgcttgc gctcgcgcga
     1801 gcggcgcagg cgcggggcga agtgccg
//
